TY  - JOUR
AU  - Uzelac, Iva
AU  - Avramov, Miloš
AU  - Čelić, Tatjana
AU  - Vukašinović, Elvira
AU  - Gošić-Dondo, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Blagojević, Duško
AU  - Popović, Željko D.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3706
AB  - The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes – citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.
PB  - Nature Research
T2  - Scientific Reports
T1  - Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-65926-w
SP  - 1
EP  - 11
ER  - 

@article{
author = "Uzelac, Iva and Avramov, Miloš and Čelić, Tatjana and Vukašinović, Elvira and Gošić-Dondo, Snežana and Purać, Jelena and Kojić, Danijela and Blagojević, Duško and Popović, Željko D.",
year = "2020",
abstract = "The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes – citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-65926-w",
pages = "1-11"
}

Uzelac, I., Avramov, M., Čelić, T., Vukašinović, E., Gošić-Dondo, S., Purać, J., Kojić, D., Blagojević, D.,& Popović, Ž. D.. (2020). Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.). in Scientific Reports
Nature Research., 10(1), 1-11.
https://doi.org/10.1038/s41598-020-65926-w

Uzelac I, Avramov M, Čelić T, Vukašinović E, Gošić-Dondo S, Purać J, Kojić D, Blagojević D, Popović ŽD. Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.). in Scientific Reports. 2020;10(1):1-11.
doi:10.1038/s41598-020-65926-w .

Uzelac, Iva, Avramov, Miloš, Čelić, Tatjana, Vukašinović, Elvira, Gošić-Dondo, Snežana, Purać, Jelena, Kojić, Danijela, Blagojević, Duško, Popović, Željko D., "Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)" in Scientific Reports, 10, no. 1 (2020):1-11,
https://doi.org/10.1038/s41598-020-65926-w . .

TY  - JOUR
AU  - Vukašinović, Elvira L.
AU  - Čelić, Tatjana V.
AU  - Kojić, Danijela
AU  - Franeta, Filip
AU  - Milić, Stanko
AU  - Ninkov, Jordana
AU  - Blagojević, Duško
AU  - Purać, Jelena
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0045653519326153?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3536
AB  - In this study the effect of long term exposure to cadmium (Cd) on Ostrinia nubilalis larval growth, development, survival rate and oxidative status was analyzed. Newly hatched first instar - L1 larvae were reared on a Cd contaminated diet until the larvae reached the final, fifth instar - L5 or developed into pupae. In total, six experimental groups, five treatments (concentrations of Cd in fresh diet: Cd I: 0.73, Cd II: 3.70, Cd III: 6.85, Cd IV: 41.71 and Cd V: 77.53 mg kg-1) and a control group (C) were set up. The results of the experiment showed that exposure to higher concentrations of Cd (41.71 and 77.53 mg kg-1) had a significant influence on development and redox status of O. nubilalis larvae: (1) the development rate was strongly reduced resulting in a prolonged pupation time; (2) the survival rate of larvae was prominently lower; (3) bioaccumulation factor (measured in pupae) was reduced which indicated that larvae could accumulate Cd to a certain level; (4) the level of the lipid peroxidation was significantly higher, which points to oxidative damage; (5) the expression of Mtn was significantly up-regulated while Cat and GPx genes down-regulated. In conclusion, long term exposure to dietary Cd in a concentration of 41.7 mg kg-1 and higher, induced oxidative stress and slowed down growth and development of O. nubilalis larvae.
T2  - Chemosphere
T1  - The effect of long term exposure to cadmium on Ostrinia nubilalis growth, development, survival rate and oxidative status.
VL  - 243
DO  - 10.1016/j.chemosphere.2019.125375
SP  - 125375
ER  - 

@article{
author = "Vukašinović, Elvira L. and Čelić, Tatjana V. and Kojić, Danijela and Franeta, Filip and Milić, Stanko and Ninkov, Jordana and Blagojević, Duško and Purać, Jelena",
year = "2020",
abstract = "In this study the effect of long term exposure to cadmium (Cd) on Ostrinia nubilalis larval growth, development, survival rate and oxidative status was analyzed. Newly hatched first instar - L1 larvae were reared on a Cd contaminated diet until the larvae reached the final, fifth instar - L5 or developed into pupae. In total, six experimental groups, five treatments (concentrations of Cd in fresh diet: Cd I: 0.73, Cd II: 3.70, Cd III: 6.85, Cd IV: 41.71 and Cd V: 77.53 mg kg-1) and a control group (C) were set up. The results of the experiment showed that exposure to higher concentrations of Cd (41.71 and 77.53 mg kg-1) had a significant influence on development and redox status of O. nubilalis larvae: (1) the development rate was strongly reduced resulting in a prolonged pupation time; (2) the survival rate of larvae was prominently lower; (3) bioaccumulation factor (measured in pupae) was reduced which indicated that larvae could accumulate Cd to a certain level; (4) the level of the lipid peroxidation was significantly higher, which points to oxidative damage; (5) the expression of Mtn was significantly up-regulated while Cat and GPx genes down-regulated. In conclusion, long term exposure to dietary Cd in a concentration of 41.7 mg kg-1 and higher, induced oxidative stress and slowed down growth and development of O. nubilalis larvae.",
journal = "Chemosphere",
title = "The effect of long term exposure to cadmium on Ostrinia nubilalis growth, development, survival rate and oxidative status.",
volume = "243",
doi = "10.1016/j.chemosphere.2019.125375",
pages = "125375"
}

Vukašinović, E. L., Čelić, T. V., Kojić, D., Franeta, F., Milić, S., Ninkov, J., Blagojević, D.,& Purać, J.. (2020). The effect of long term exposure to cadmium on Ostrinia nubilalis growth, development, survival rate and oxidative status.. in Chemosphere, 243, 125375.
https://doi.org/10.1016/j.chemosphere.2019.125375

Vukašinović EL, Čelić TV, Kojić D, Franeta F, Milić S, Ninkov J, Blagojević D, Purać J. The effect of long term exposure to cadmium on Ostrinia nubilalis growth, development, survival rate and oxidative status.. in Chemosphere. 2020;243:125375.
doi:10.1016/j.chemosphere.2019.125375 .

Vukašinović, Elvira L., Čelić, Tatjana V., Kojić, Danijela, Franeta, Filip, Milić, Stanko, Ninkov, Jordana, Blagojević, Duško, Purać, Jelena, "The effect of long term exposure to cadmium on Ostrinia nubilalis growth, development, survival rate and oxidative status." in Chemosphere, 243 (2020):125375,
https://doi.org/10.1016/j.chemosphere.2019.125375 . .

TY  - CONF
AU  - Savić, Danijela
AU  - Opačić, Miloš
AU  - Brkljačić, Jelena
AU  - Ristić, Aleksandar
AU  - Sokić, Dragoslav
AU  - Baščarević, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Zorović, Maja
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasić, Snežana
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5882
AB  - More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s
well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the
amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal
biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe
epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques,
optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals,
we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of
neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper
metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into
the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper
is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further
investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal
to pathology of mTLE or they represent epiphenomenon.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - The importance of copper in pathology of mesial temporal lobe epilepsy
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5882
ER  - 

@conference{
author = "Savić, Danijela and Opačić, Miloš and Brkljačić, Jelena and Ristić, Aleksandar and Sokić, Dragoslav and Baščarević, Vladimir and Raičević, Savo and Savić, Slobodan and Zorović, Maja and Živin, Marko and Šelih, Vid Simon and Spasić, Snežana and Spasojević, Ivan",
year = "2019",
abstract = "More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s
well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the
amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal
biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe
epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques,
optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals,
we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of
neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper
metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into
the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper
is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further
investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal
to pathology of mTLE or they represent epiphenomenon.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "The importance of copper in pathology of mesial temporal lobe epilepsy",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5882"
}

Savić, D., Opačić, M., Brkljačić, J., Ristić, A., Sokić, D., Baščarević, V., Raičević, S., Savić, S., Zorović, M., Živin, M., Šelih, V. S., Spasić, S.,& Spasojević, I.. (2019). The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 46.
https://hdl.handle.net/21.15107/rcub_ibiss_5882

Savić D, Opačić M, Brkljačić J, Ristić A, Sokić D, Baščarević V, Raičević S, Savić S, Zorović M, Živin M, Šelih VS, Spasić S, Spasojević I. The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:46.
https://hdl.handle.net/21.15107/rcub_ibiss_5882 .

Savić, Danijela, Opačić, Miloš, Brkljačić, Jelena, Ristić, Aleksandar, Sokić, Dragoslav, Baščarević, Vladimir, Raičević, Savo, Savić, Slobodan, Zorović, Maja, Živin, Marko, Šelih, Vid Simon, Spasić, Snežana, Spasojević, Ivan, "The importance of copper in pathology of mesial temporal lobe epilepsy" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):46,
https://hdl.handle.net/21.15107/rcub_ibiss_5882 .

TY  - CONF
AU  - Opačić, Miloš
AU  - Zorović, Maja
AU  - Savić, Danijela
AU  - Živin, Marko
AU  - Raičević, Savo
AU  - Baščarević, Vladimir
AU  - Ristić, Aleksandar
AU  - Sokić, Dragoslav
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5986
AB  - Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients
SP  - 296
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5986
ER  - 

@conference{
author = "Opačić, Miloš and Zorović, Maja and Savić, Danijela and Živin, Marko and Raičević, Savo and Baščarević, Vladimir and Ristić, Aleksandar and Sokić, Dragoslav and Spasojević, Ivan",
year = "2019",
abstract = "Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients",
pages = "296",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5986"
}

Opačić, M., Zorović, M., Savić, D., Živin, M., Raičević, S., Baščarević, V., Ristić, A., Sokić, D.,& Spasojević, I.. (2019). Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 296.
https://hdl.handle.net/21.15107/rcub_ibiss_5986

Opačić M, Zorović M, Savić D, Živin M, Raičević S, Baščarević V, Ristić A, Sokić D, Spasojević I. Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:296.
https://hdl.handle.net/21.15107/rcub_ibiss_5986 .

Opačić, Miloš, Zorović, Maja, Savić, Danijela, Živin, Marko, Raičević, Savo, Baščarević, Vladimir, Ristić, Aleksandar, Sokić, Dragoslav, Spasojević, Ivan, "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):296,
https://hdl.handle.net/21.15107/rcub_ibiss_5986 .

TY  - CONF
AU  - Ristić, Aleksandar
AU  - Savić, Danijela
AU  - Bogdanović Pristov, Jelena
AU  - Brkljačić, Jelena
AU  - Baščarević, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5991
AB  - Mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS) is probably the single most frequent human focal epilepsy. The involvement of redox processes in the pathological mechanisms of mTLE-HS has been implicated  by mitochondrial dysfunction and oxidative damage, and by different metabolic abnormalities that have been observed in sclerotic hippocampi, such as altered metabolism of redox-active metals. The strongest proof came with the analysis of enzymatic antioxidative system. Sclerotic hippocampi show drastically increased activity and levels of hydrogen peroxide–removing enzymes – catalase and glutathione peroxidase/reductase. Catalase is located mainly in neurons in both, controls and HS. Sclerotic hippocampi are depleted of glutathione peroxidase-positive blood vessels that are present in control hippocampi. Pertinent to this, it has been documented that hippocampi of mTLE-HS patients show increased blood vessel density, but most of the vessels represent atrophic vascular structures. On the other hand, HS showes specific glutathione peroxidase-rich loci that are present in gyrus dentatus, CA regions, and alveus, and appear to represent bundles of astrocytes. These loci are probably sites of excessive (neuronal) production of hydrogen peroxide that is counteracted by astrocytes. Finally, protein levels of mitochondrial enzyme manganese superoxide dismutase are higher in HS than controls. Neurons with abnormal morphology and strong superoxide dismutase immunofluorescence are present in all neuronal layers in HS. In close, antioxidative system is upregulated in HS implying that epileptogenic hippocampi are exposed to oxidative stress. The involvement of redox alterations in the pathology of epilepsy may open new pharmacologic perspectives for mTLE-HS treatment.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Hippocampal Antioxidative System in Epilepsy
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5991
ER  - 

@conference{
author = "Ristić, Aleksandar and Savić, Danijela and Bogdanović Pristov, Jelena and Brkljačić, Jelena and Baščarević, Vladimir and Raičević, Savo and Savić, Slobodan",
year = "2019",
abstract = "Mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS) is probably the single most frequent human focal epilepsy. The involvement of redox processes in the pathological mechanisms of mTLE-HS has been implicated  by mitochondrial dysfunction and oxidative damage, and by different metabolic abnormalities that have been observed in sclerotic hippocampi, such as altered metabolism of redox-active metals. The strongest proof came with the analysis of enzymatic antioxidative system. Sclerotic hippocampi show drastically increased activity and levels of hydrogen peroxide–removing enzymes – catalase and glutathione peroxidase/reductase. Catalase is located mainly in neurons in both, controls and HS. Sclerotic hippocampi are depleted of glutathione peroxidase-positive blood vessels that are present in control hippocampi. Pertinent to this, it has been documented that hippocampi of mTLE-HS patients show increased blood vessel density, but most of the vessels represent atrophic vascular structures. On the other hand, HS showes specific glutathione peroxidase-rich loci that are present in gyrus dentatus, CA regions, and alveus, and appear to represent bundles of astrocytes. These loci are probably sites of excessive (neuronal) production of hydrogen peroxide that is counteracted by astrocytes. Finally, protein levels of mitochondrial enzyme manganese superoxide dismutase are higher in HS than controls. Neurons with abnormal morphology and strong superoxide dismutase immunofluorescence are present in all neuronal layers in HS. In close, antioxidative system is upregulated in HS implying that epileptogenic hippocampi are exposed to oxidative stress. The involvement of redox alterations in the pathology of epilepsy may open new pharmacologic perspectives for mTLE-HS treatment.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Hippocampal Antioxidative System in Epilepsy",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5991"
}

Ristić, A., Savić, D., Bogdanović Pristov, J., Brkljačić, J., Baščarević, V., Raičević, S.,& Savić, S.. (2019). Hippocampal Antioxidative System in Epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 42.
https://hdl.handle.net/21.15107/rcub_ibiss_5991

Ristić A, Savić D, Bogdanović Pristov J, Brkljačić J, Baščarević V, Raičević S, Savić S. Hippocampal Antioxidative System in Epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:42.
https://hdl.handle.net/21.15107/rcub_ibiss_5991 .

Ristić, Aleksandar, Savić, Danijela, Bogdanović Pristov, Jelena, Brkljačić, Jelena, Baščarević, Vladimir, Raičević, Savo, Savić, Slobodan, "Hippocampal Antioxidative System in Epilepsy" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):42,
https://hdl.handle.net/21.15107/rcub_ibiss_5991 .

TY  - CONF
AU  - Blagojević, Duško
AU  - Nikolić-Kokić, Aleksandra
AU  - Tatalović, Nikola
AU  - Oreščanin Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Spasić, Mihajlo
AU  - Miljević, Čedo
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4278
AB  - Different studies reported that patients with schizophrenia had lower cholesterol levels in blood compared to healthy controls. However, it is unclear whether changed cholesterol concentration and lipid status are a consequence of changed neurotransmitter metabolism intrinsic to origin of the disease or affect central nervous system neurotransmission and influence the development of psychiatric disorders. Anyway, schizophrenia treatment with atypical antipsychotic drugs (APD) additionally influences lipid status in blood and all families of APD agents can cause severe side effects including dyslipidemia. Therefore, the aim of the present study was to evaluate effects of 28-day treatment with recommended human daily dose of APD: ziprasidone, clozapine, sertindole on 3 months old healthy male rats’ levels of cholesterol, HDL, LDL, and triglyceride in the blood serum. Our results showed a decrease of both triglycerides and cholesterol in clozapine treated rats. In ziprasidone treated rats triglycerides and HDL were lower comparing to untreated controls. Treatment with sertindole had no effects on lipid blood serum levels. However, there were no changes of index of atherosclerosis in APD treated rats. Our results showed that treatment with clozapine and ziprasidone influence blood serum levels of lipids indicating altered lipid metabolism.
PB  - Bologna: Federation of European Physiological Societies
C3  - Joint Meeting of the Federation of European Physiological Societies (FEPS) and the Italian Physiological Society (SIF); 2019 Sep 10-13; Bologna, Italy
T1  - The effects of clozapine, ziprasidone and sertindole treatment on lipid profile in rats
IS  - Supplement 718
VL  - 227
DO  - 10.1111/apha.13366
SP  - 85
EP  - 85
ER  - 

@conference{
author = "Blagojević, Duško and Nikolić-Kokić, Aleksandra and Tatalović, Nikola and Oreščanin Dušić, Zorana and Vidonja Uzelac, Teodora and Spasić, Mihajlo and Miljević, Čedo",
year = "2019",
abstract = "Different studies reported that patients with schizophrenia had lower cholesterol levels in blood compared to healthy controls. However, it is unclear whether changed cholesterol concentration and lipid status are a consequence of changed neurotransmitter metabolism intrinsic to origin of the disease or affect central nervous system neurotransmission and influence the development of psychiatric disorders. Anyway, schizophrenia treatment with atypical antipsychotic drugs (APD) additionally influences lipid status in blood and all families of APD agents can cause severe side effects including dyslipidemia. Therefore, the aim of the present study was to evaluate effects of 28-day treatment with recommended human daily dose of APD: ziprasidone, clozapine, sertindole on 3 months old healthy male rats’ levels of cholesterol, HDL, LDL, and triglyceride in the blood serum. Our results showed a decrease of both triglycerides and cholesterol in clozapine treated rats. In ziprasidone treated rats triglycerides and HDL were lower comparing to untreated controls. Treatment with sertindole had no effects on lipid blood serum levels. However, there were no changes of index of atherosclerosis in APD treated rats. Our results showed that treatment with clozapine and ziprasidone influence blood serum levels of lipids indicating altered lipid metabolism.",
publisher = "Bologna: Federation of European Physiological Societies",
journal = "Joint Meeting of the Federation of European Physiological Societies (FEPS) and the Italian Physiological Society (SIF); 2019 Sep 10-13; Bologna, Italy",
title = "The effects of clozapine, ziprasidone and sertindole treatment on lipid profile in rats",
number = "Supplement 718",
volume = "227",
doi = "10.1111/apha.13366",
pages = "85-85"
}

Blagojević, D., Nikolić-Kokić, A., Tatalović, N., Oreščanin Dušić, Z., Vidonja Uzelac, T., Spasić, M.,& Miljević, Č.. (2019). The effects of clozapine, ziprasidone and sertindole treatment on lipid profile in rats. in Joint Meeting of the Federation of European Physiological Societies (FEPS) and the Italian Physiological Society (SIF); 2019 Sep 10-13; Bologna, Italy
Bologna: Federation of European Physiological Societies., 227(Supplement 718), 85-85.
https://doi.org/10.1111/apha.13366

Blagojević D, Nikolić-Kokić A, Tatalović N, Oreščanin Dušić Z, Vidonja Uzelac T, Spasić M, Miljević Č. The effects of clozapine, ziprasidone and sertindole treatment on lipid profile in rats. in Joint Meeting of the Federation of European Physiological Societies (FEPS) and the Italian Physiological Society (SIF); 2019 Sep 10-13; Bologna, Italy. 2019;227(Supplement 718):85-85.
doi:10.1111/apha.13366 .

Blagojević, Duško, Nikolić-Kokić, Aleksandra, Tatalović, Nikola, Oreščanin Dušić, Zorana, Vidonja Uzelac, Teodora, Spasić, Mihajlo, Miljević, Čedo, "The effects of clozapine, ziprasidone and sertindole treatment on lipid profile in rats" in Joint Meeting of the Federation of European Physiological Societies (FEPS) and the Italian Physiological Society (SIF); 2019 Sep 10-13; Bologna, Italy, 227, no. Supplement 718 (2019):85-85,
https://doi.org/10.1111/apha.13366 . .

TY  - JOUR
AU  - Vidonja Uzelac, Teodora
AU  - Tatalović, Nikola
AU  - Mijović, Milica
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Bresjanac, Mara
AU  - Blagojević, Duško
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900006V
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3984
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3395
AB  - Our previous results showed that a single oral dose (1 or 20 mg/kg body weight) of the anti-addiction agent ibogaine induced in rats 6 and 24 h after administration glycogenolytic activity in hepatocytes, followed by a mild oxidative stress. In this work, we examined the in vivo effect of the same doses of ibogaine on rat kidney morphology, antioxidant enzyme (superoxide dismutases (SOD1 and 2), catalase, glutathione peroxidase, glutathione reductase (GR) and glutathione- S-transferase) activities, and oxidative stress (TBARS) and redox (-SH groups) parameters. The dose of 1 mg/kg ibogaine induced an elevation in SOD1 activity and decreased GR activity after 6 and 24 h. GR activity was decreased at 6 and 24 h after 20 mg/kg ibogaine administration, suggesting changed redox homeostasis. After 24 h, we observed an increase in moderate morphological changes, without changes in urinalyses, indicating that kidney function was not measurably affected. Nevertheless, kidney-function monitoring during and following ibogaine use in human subjects is advisable.
T2  - Archives of Biological Sciences
T1  - Effects of ibogaine per os treatment on redox homeostasis in rat kidney
IS  - 2
VL  - 71
DO  - 10.2298/ABS190208006V
SP  - 245
EP  - 252
ER  - 

@article{
author = "Vidonja Uzelac, Teodora and Tatalović, Nikola and Mijović, Milica and Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Bresjanac, Mara and Blagojević, Duško",
year = "2019",
abstract = "Our previous results showed that a single oral dose (1 or 20 mg/kg body weight) of the anti-addiction agent ibogaine induced in rats 6 and 24 h after administration glycogenolytic activity in hepatocytes, followed by a mild oxidative stress. In this work, we examined the in vivo effect of the same doses of ibogaine on rat kidney morphology, antioxidant enzyme (superoxide dismutases (SOD1 and 2), catalase, glutathione peroxidase, glutathione reductase (GR) and glutathione- S-transferase) activities, and oxidative stress (TBARS) and redox (-SH groups) parameters. The dose of 1 mg/kg ibogaine induced an elevation in SOD1 activity and decreased GR activity after 6 and 24 h. GR activity was decreased at 6 and 24 h after 20 mg/kg ibogaine administration, suggesting changed redox homeostasis. After 24 h, we observed an increase in moderate morphological changes, without changes in urinalyses, indicating that kidney function was not measurably affected. Nevertheless, kidney-function monitoring during and following ibogaine use in human subjects is advisable.",
journal = "Archives of Biological Sciences",
title = "Effects of ibogaine per os treatment on redox homeostasis in rat kidney",
number = "2",
volume = "71",
doi = "10.2298/ABS190208006V",
pages = "245-252"
}

Vidonja Uzelac, T., Tatalović, N., Mijović, M., Nikolić-Kokić, A., Oreščanin Dušić, Z., Bresjanac, M.,& Blagojević, D.. (2019). Effects of ibogaine per os treatment on redox homeostasis in rat kidney. in Archives of Biological Sciences, 71(2), 245-252.
https://doi.org/10.2298/ABS190208006V

Vidonja Uzelac T, Tatalović N, Mijović M, Nikolić-Kokić A, Oreščanin Dušić Z, Bresjanac M, Blagojević D. Effects of ibogaine per os treatment on redox homeostasis in rat kidney. in Archives of Biological Sciences. 2019;71(2):245-252.
doi:10.2298/ABS190208006V .

Vidonja Uzelac, Teodora, Tatalović, Nikola, Mijović, Milica, Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Bresjanac, Mara, Blagojević, Duško, "Effects of ibogaine per os treatment on redox homeostasis in rat kidney" in Archives of Biological Sciences, 71, no. 2 (2019):245-252,
https://doi.org/10.2298/ABS190208006V . .

TY  - JOUR
AU  - Radić, I
AU  - Mijović, M
AU  - Tatalović, Nikola
AU  - Mitić, M
AU  - Lukić, V
AU  - Joksimović, B
AU  - Petrović, Z
AU  - Ristić, S
AU  - Veličković, S
AU  - Nestorovic, V
AU  - Corac, A
AU  - Mirić, M
AU  - Adžić, M
AU  - Blagojević, Duško
AU  - Popović, L
AU  - Hudomal, S J
PY  - 2019
UR  - http://journals.sagepub.com/doi/10.1177/0960327119829518
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3281
AB  - In 2012, alcohol liver disease resulted in 3.3 million-5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey ( per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper-zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol ingestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling.
T2  - Human & Experimental Toxicology
T1  - Protective effects of whey on rat liver damage induced by chronic alcohol intake.
DO  - 10.1177/0960327119829518
ER  - 

@article{
author = "Radić, I and Mijović, M and Tatalović, Nikola and Mitić, M and Lukić, V and Joksimović, B and Petrović, Z and Ristić, S and Veličković, S and Nestorovic, V and Corac, A and Mirić, M and Adžić, M and Blagojević, Duško and Popović, L and Hudomal, S J",
year = "2019",
abstract = "In 2012, alcohol liver disease resulted in 3.3 million-5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey ( per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper-zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol ingestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling.",
journal = "Human & Experimental Toxicology",
title = "Protective effects of whey on rat liver damage induced by chronic alcohol intake.",
doi = "10.1177/0960327119829518"
}

Radić, I., Mijović, M., Tatalović, N., Mitić, M., Lukić, V., Joksimović, B., Petrović, Z., Ristić, S., Veličković, S., Nestorovic, V., Corac, A., Mirić, M., Adžić, M., Blagojević, D., Popović, L.,& Hudomal, S. J.. (2019). Protective effects of whey on rat liver damage induced by chronic alcohol intake.. in Human & Experimental Toxicology.
https://doi.org/10.1177/0960327119829518

Radić I, Mijović M, Tatalović N, Mitić M, Lukić V, Joksimović B, Petrović Z, Ristić S, Veličković S, Nestorovic V, Corac A, Mirić M, Adžić M, Blagojević D, Popović L, Hudomal SJ. Protective effects of whey on rat liver damage induced by chronic alcohol intake.. in Human & Experimental Toxicology. 2019;.
doi:10.1177/0960327119829518 .

Radić, I, Mijović, M, Tatalović, Nikola, Mitić, M, Lukić, V, Joksimović, B, Petrović, Z, Ristić, S, Veličković, S, Nestorovic, V, Corac, A, Mirić, M, Adžić, M, Blagojević, Duško, Popović, L, Hudomal, S J, "Protective effects of whey on rat liver damage induced by chronic alcohol intake." in Human & Experimental Toxicology (2019),
https://doi.org/10.1177/0960327119829518 . .

TY  - JOUR
AU  - Uzelac, Tamara N.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana D.
AU  - Mačvanin, Mirjana T.
AU  - Nikolić, Milan R.
AU  - Mandić, Ljuba M.
AU  - Jovanović, Vesna B.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0009279719310257?via%3Dihub
UR  - http://www.ncbi.nlm.nih.gov/pubmed/31400341
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3454
AB  - Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.
T2  - Chemico-Biological Interactions
T1  - Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content.
VL  - 311
DO  - 10.1016/j.cbi.2019.108787
SP  - 108787
ER  - 

@article{
author = "Uzelac, Tamara N. and Nikolić-Kokić, Aleksandra and Spasić, Snežana D. and Mačvanin, Mirjana T. and Nikolić, Milan R. and Mandić, Ljuba M. and Jovanović, Vesna B.",
year = "2019",
abstract = "Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.",
journal = "Chemico-Biological Interactions",
title = "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content.",
volume = "311",
doi = "10.1016/j.cbi.2019.108787",
pages = "108787"
}

Uzelac, T. N., Nikolić-Kokić, A., Spasić, S. D., Mačvanin, M. T., Nikolić, M. R., Mandić, L. M.,& Jovanović, V. B.. (2019). Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content.. in Chemico-Biological Interactions, 311, 108787.
https://doi.org/10.1016/j.cbi.2019.108787

Uzelac TN, Nikolić-Kokić A, Spasić SD, Mačvanin MT, Nikolić MR, Mandić LM, Jovanović VB. Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content.. in Chemico-Biological Interactions. 2019;311:108787.
doi:10.1016/j.cbi.2019.108787 .

Uzelac, Tamara N., Nikolić-Kokić, Aleksandra, Spasić, Snežana D., Mačvanin, Mirjana T., Nikolić, Milan R., Mandić, Ljuba M., Jovanović, Vesna B., "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content." in Chemico-Biological Interactions, 311 (2019):108787,
https://doi.org/10.1016/j.cbi.2019.108787 . .

TY  - CONF
AU  - Tatalović, Nikola
AU  - Vidonja Uzelac, Teodora
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S2451830119319119?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/123456789/3898
AB  - Ibogaine is the main alkaloid in the root bark of Tabernanthe iboga plant which groves in Western-Central Africa. It has been used by natives to overcome fatigue, hunger and thirst, and in higher doses to provoke hallucinations. In the “ibogaine medical subculture” in Europe and America it is used to facilitate abstinence from variety of addictive substances (cocaine, heroin, methadone, alcohol…). It was hypothesized that adaptive changes in ATP-related cell energy homeostasis are important contributing factor, to ibogaine’s anti-addictive activity. Examinations on different experimental models showed that ibogaine caused rapid depletion of ATP accompanied by increased production of reactive oxygen species and the activation of antioxidative enzymes, as well as upregulation of energy related enzymes. Our goal was to investigate the effects of ibogaine oral application on redox balance in rat brain and energy metabolism in liver. The later was estimated by accessing amount of glycogen reserves. The null hypothesis was that ibogaine had no effect on the activity of antioxidative enzymes, concentration of lipid peroxides and free sulfhydryl groups in rat brain, or on the amount of glycogen in liver. In this study 3-month-old female Wistar rats were treated with a single dose 20 mg/kg body weight of ibogaine via gavage. Rats were sacrificed 6 or 24 h after treatment; brain and perfunded liver samples were homogenised and sonicated. Liver sample was also prepared for histological analysis. We measured the activities of antioxidative enzymes, namely total superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the activity of glutathione S-transferases (GSTs) a xenobiotic detoxification enzyme family member. Concentrations of thiobarbituric acid reactive substances (TBARS) and protein and nonprotein free sulfhydryl groups (–SH) were measured in brain sonicates. Amount of glycogen in liver was determined based on PAS staining. Results were analysed by One-way ANOVA with Tukey's HSD post hoc test, p<0.05. There were no significant changes of measured antioxidative enzymes activity in brain neither 6 nor 24 h after treatment with ibogaine. Also, total activity of GSTs remained unaltered. On the other hand, concentration of TBARS was significantly increased 6 h after treatment while after 24 h TBARS concentration was the same as in controls. Treatment with ibogaine caused an increase of protein free –SH groups concentration which was more pronounced after 24 h. However, the concentration of nonprotein free –SH groups was decreased in brain of treated rats but also more prominently after 24 h. Histological analysis of liver showed that amount of glycogen was decreased in treated rats. Glycogen depletion in the liver of treated rats was greater in 6 h group compared to 24 h group. Increased concentrations of TBARS suggest increased lipid in brain of treated rats. Decreased concentration of nonprotein free –SH groups suggests decreased concentration of reduced glutathione, the main source of nonprotein –SH groups in cells. These findings coincide with ibogaine-induced transient burst in cellular respiration followed by intensive production of reactive oxygen species, described in literature. Despite these indicators of oxidative stress there is no change in the activity of any antioxidative enzyme, not even after 6 h. Unaltered activity of GSTs suggest that applied dose of ibogaine doesn’t have an acute toxic effect on brain. Finally, alterations in glycogen amount in hepatocytes suggest a transient depletion of energy reserves, which are on their way to recovery already 24 h after treatment. All of the aforementioned suggest that after oral administration of ibogaine there is rapid transient changes in redox and energetic homeostasis in brain similar to those described on other experimental models. It seems that antioxidative defense system is capable to preserve redox homeostasis within first 6 h, but some consequences in the form of oxidative damage are still present. Since these are all energy-intensive processes, required energy may have been obtained at the expense of liver glycogen. Having in mind a proposed role of energetic and redox related changes in anti-addictive effects of ibogaine, it is essential to investigate redox balance and energy metabolism in brain within the first hours after ibogaine application.
PB  - Elsevier Ltd.
C3  - IBRO Reports
T1  - Effects of ibogaine treatment on redox homeostasis and energy metabolism in rat
IS  - Supplement
VL  - 7
DO  - 10.1016/j.ibror.2019.09.087
SP  - S43
ER  - 

@conference{
author = "Tatalović, Nikola and Vidonja Uzelac, Teodora and Oreščanin Dušić, Zorana and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo and Blagojević, Duško",
year = "2019",
abstract = "Ibogaine is the main alkaloid in the root bark of Tabernanthe iboga plant which groves in Western-Central Africa. It has been used by natives to overcome fatigue, hunger and thirst, and in higher doses to provoke hallucinations. In the “ibogaine medical subculture” in Europe and America it is used to facilitate abstinence from variety of addictive substances (cocaine, heroin, methadone, alcohol…). It was hypothesized that adaptive changes in ATP-related cell energy homeostasis are important contributing factor, to ibogaine’s anti-addictive activity. Examinations on different experimental models showed that ibogaine caused rapid depletion of ATP accompanied by increased production of reactive oxygen species and the activation of antioxidative enzymes, as well as upregulation of energy related enzymes. Our goal was to investigate the effects of ibogaine oral application on redox balance in rat brain and energy metabolism in liver. The later was estimated by accessing amount of glycogen reserves. The null hypothesis was that ibogaine had no effect on the activity of antioxidative enzymes, concentration of lipid peroxides and free sulfhydryl groups in rat brain, or on the amount of glycogen in liver. In this study 3-month-old female Wistar rats were treated with a single dose 20 mg/kg body weight of ibogaine via gavage. Rats were sacrificed 6 or 24 h after treatment; brain and perfunded liver samples were homogenised and sonicated. Liver sample was also prepared for histological analysis. We measured the activities of antioxidative enzymes, namely total superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the activity of glutathione S-transferases (GSTs) a xenobiotic detoxification enzyme family member. Concentrations of thiobarbituric acid reactive substances (TBARS) and protein and nonprotein free sulfhydryl groups (–SH) were measured in brain sonicates. Amount of glycogen in liver was determined based on PAS staining. Results were analysed by One-way ANOVA with Tukey's HSD post hoc test, p<0.05. There were no significant changes of measured antioxidative enzymes activity in brain neither 6 nor 24 h after treatment with ibogaine. Also, total activity of GSTs remained unaltered. On the other hand, concentration of TBARS was significantly increased 6 h after treatment while after 24 h TBARS concentration was the same as in controls. Treatment with ibogaine caused an increase of protein free –SH groups concentration which was more pronounced after 24 h. However, the concentration of nonprotein free –SH groups was decreased in brain of treated rats but also more prominently after 24 h. Histological analysis of liver showed that amount of glycogen was decreased in treated rats. Glycogen depletion in the liver of treated rats was greater in 6 h group compared to 24 h group. Increased concentrations of TBARS suggest increased lipid in brain of treated rats. Decreased concentration of nonprotein free –SH groups suggests decreased concentration of reduced glutathione, the main source of nonprotein –SH groups in cells. These findings coincide with ibogaine-induced transient burst in cellular respiration followed by intensive production of reactive oxygen species, described in literature. Despite these indicators of oxidative stress there is no change in the activity of any antioxidative enzyme, not even after 6 h. Unaltered activity of GSTs suggest that applied dose of ibogaine doesn’t have an acute toxic effect on brain. Finally, alterations in glycogen amount in hepatocytes suggest a transient depletion of energy reserves, which are on their way to recovery already 24 h after treatment. All of the aforementioned suggest that after oral administration of ibogaine there is rapid transient changes in redox and energetic homeostasis in brain similar to those described on other experimental models. It seems that antioxidative defense system is capable to preserve redox homeostasis within first 6 h, but some consequences in the form of oxidative damage are still present. Since these are all energy-intensive processes, required energy may have been obtained at the expense of liver glycogen. Having in mind a proposed role of energetic and redox related changes in anti-addictive effects of ibogaine, it is essential to investigate redox balance and energy metabolism in brain within the first hours after ibogaine application.",
publisher = "Elsevier Ltd.",
journal = "IBRO Reports",
title = "Effects of ibogaine treatment on redox homeostasis and energy metabolism in rat",
number = "Supplement",
volume = "7",
doi = "10.1016/j.ibror.2019.09.087",
pages = "S43"
}

Tatalović, N., Vidonja Uzelac, T., Oreščanin Dušić, Z., Nikolić-Kokić, A., Spasić, M.,& Blagojević, D.. (2019). Effects of ibogaine treatment on redox homeostasis and energy metabolism in rat. in IBRO Reports
Elsevier Ltd.., 7(Supplement), S43.
https://doi.org/10.1016/j.ibror.2019.09.087

Tatalović N, Vidonja Uzelac T, Oreščanin Dušić Z, Nikolić-Kokić A, Spasić M, Blagojević D. Effects of ibogaine treatment on redox homeostasis and energy metabolism in rat. in IBRO Reports. 2019;7(Supplement):S43.
doi:10.1016/j.ibror.2019.09.087 .

Tatalović, Nikola, Vidonja Uzelac, Teodora, Oreščanin Dušić, Zorana, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, Blagojević, Duško, "Effects of ibogaine treatment on redox homeostasis and energy metabolism in rat" in IBRO Reports, 7, no. Supplement (2019):S43,
https://doi.org/10.1016/j.ibror.2019.09.087 . .

TY  - THES
AU  - Vidonja Uzelac, Teodora
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3473
AB  - Ibogain, primenjen u jediničnoj oralnoj dozi (1-25 mg/kg telesne mase (t.m)) se koristi kao agens u sprečavanju različitih oblika zavisnosti kod ljudi. Njegovi efekti traju i do 72 h. Ex vivo rezultati pokazuju da ibogain izaziva potrošnju energije u ćelijama i njenu restituciju, što je praćeno povećanjem stvaranja reaktivnih vrsta kiseonika i aktivnosti antioksidativne odbrane. Stoga je cilj ovog rada bio da istraži efekte jedinične oralne doze ibogaina (1 ili 20 mg/kg t.m.) na antioksidativnu odbranu u različitim tkivima pacova. Posle 6 i 24 h nakon ingestije ibogaina, histološka ispitivanja su pokazala pojačanu glikogenolitičku aktivnost hepatocita, koja je bila najviša posle 24 h kod životinja koje su primile 20 mg/kg ibogaina. Nije bilo promena u aktivnosti antioksidativnih enzimani u jetri ni u eritrocitima u tim periodima što ukazuje na izostanak sistemskog efekta na nivou oksidativnog pritiska. U jetri se povećala aktivnost ksantin oksidaze kod pacova koji su primili ibogain u dozi od 20 mg/kg u poređenju sa kontrolama što ukazuje na brži obrt adenozina. Nivo TBARS u jetri je povišen kod grupe tretirane sa 1 mg/kg posle 24 h u poređenju sa kontrolama što sugeriše postojanje umerenog oksidativnog stresa. U srcu je utvrđeno da ibogain nakon 6 h smanjuje količinu proteinskih -SH grupa što ukazuje na unutar-proteinsku oksidaciju, koja zajedno sa narušenom energetskom homeostazom može dovesti do promena u srčanom radu i nastanka diskretnih oštećenja. Ibogain inhibira ex vivo kontraktilnost ileuma u nivou doza ispod mM, pri čemu je efekat kod električne stimulacije, u odnosu na toničnu, značajno veći. Rezultati pretretmana na ileumu ex vivo ukazuju da ibogain svoje dejstvo ostvaruje delimično preko KATP kanala. Ibogain izaziva u ileumu ex vivo povećanje aktivnosti superoksid dismutaze (SOD) i katalaze (KAT), ali se ti efekti gube dodavanjem glibenklamida. Ibogain menja i redoks 7 ravnotežu u testisima, a nađeno povećanje aktivnosti SOD ukazuje na delikatnost očuvanja balansa koncentracija superoksida i vodonik peroksida koja se smatra krucijalnom u procesu kapacitacije spermatozoida. Rezultati pokazuju da ibogain u primenjenim dozama utiče na redoks status posmatranih tkiva i menja aktivnost antioksidativne odbrane, ali je odgovor visoko tkivno, dozno i vremenski specifičan, bez jasne zajedničke karakteristike.
AB  - Ibogaine, administered as a single oral dose (1-25 mg/kg body weight, (b.w.)), has been used as an addiction-interrupting agent. Its effects persist for up to 72 h. Ex vivo results showed that ibogaine induced cellular energy consumption and restitution, followed by increased reactive oxygen species production and antioxidant activity. Therefore, the aim of this work was to explore the effect of a single oral dose of ibogaine (1 or 20 mg/kg b.w.) on antioxidative defenses in different rat tissues. Six and 24 h after ibogaine administration, histological examination showed glycogenolytic activity in hepatocytes, which was highest after 24 h in animals that received 20 mg/kg ibogaine. There were no changes in the activities of antioxidant enzymes in the liver and erythrocytes after ibogaine treatment, regardless of the dose. These results suggest there were no systemic ibogaine effects regarding antioxidant defense. Hepatic xanthine oxidase activity was elevated in rats that received 20 mg/kg compared to the controls, suggesting faster adenosine turnover. TBARS concentration was elevated in the group treated with 1 mg/kg after 24 h compared to the controls, suggesting mild oxidative stress. It was shown that ibogaine in heart after 6 h diminished the amount of protein -SH groups suggesting intra-protein oxidation, which along disturbed energetic homeostasis could lead to discrete cellular oxidative damages and possible heart failure. Ibogaine inhibited ex vivo ileum contractile activity with doses below mM. The effect was stronger in electrically stimulated contractions than tonic. Pretreatment with different potassium channel related agents showed that ibogaine obtained its inhibitory activity partially through KATP channels. Ibogaine induced the elevation of superoxide dismutase (SOD), catalase activity 9 ex vivo, but this effect was diminished by addition of glibenclamide. Ibogaine changed redox homeostasis in testicles as well, and elevated SOD activity suggested the importance of the preservation of delicate balance of superoxide and hydrogen peroxide concentrations that is crucial in sperm capacitation. Our results showed that ibogaine in doses applied in vivo per os influenced redox state and changed antioxidant activity, but response is highly tissue-, dose- and time- specific, without clear common characteristic.
PB  - Belgrade: Faculty of Medicine, University of Belgrade
T2  - Faculty of Medicine, University of Belgrade
T1  - Efekat ibogaina na sistem antioksidativne zaštite u perifernim tkivima i kontraktilnost terminalnog ileuma kod mužjaka pacova
T1  - Effect of ibogaine on antioxidative defense system in peripheral tissues and terminal ileum contractility in male rats
SP  - 1
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3473
ER  - 

@phdthesis{
author = "Vidonja Uzelac, Teodora",
year = "2019",
abstract = "Ibogain, primenjen u jediničnoj oralnoj dozi (1-25 mg/kg telesne mase (t.m)) se koristi kao agens u sprečavanju različitih oblika zavisnosti kod ljudi. Njegovi efekti traju i do 72 h. Ex vivo rezultati pokazuju da ibogain izaziva potrošnju energije u ćelijama i njenu restituciju, što je praćeno povećanjem stvaranja reaktivnih vrsta kiseonika i aktivnosti antioksidativne odbrane. Stoga je cilj ovog rada bio da istraži efekte jedinične oralne doze ibogaina (1 ili 20 mg/kg t.m.) na antioksidativnu odbranu u različitim tkivima pacova. Posle 6 i 24 h nakon ingestije ibogaina, histološka ispitivanja su pokazala pojačanu glikogenolitičku aktivnost hepatocita, koja je bila najviša posle 24 h kod životinja koje su primile 20 mg/kg ibogaina. Nije bilo promena u aktivnosti antioksidativnih enzimani u jetri ni u eritrocitima u tim periodima što ukazuje na izostanak sistemskog efekta na nivou oksidativnog pritiska. U jetri se povećala aktivnost ksantin oksidaze kod pacova koji su primili ibogain u dozi od 20 mg/kg u poređenju sa kontrolama što ukazuje na brži obrt adenozina. Nivo TBARS u jetri je povišen kod grupe tretirane sa 1 mg/kg posle 24 h u poređenju sa kontrolama što sugeriše postojanje umerenog oksidativnog stresa. U srcu je utvrđeno da ibogain nakon 6 h smanjuje količinu proteinskih -SH grupa što ukazuje na unutar-proteinsku oksidaciju, koja zajedno sa narušenom energetskom homeostazom može dovesti do promena u srčanom radu i nastanka diskretnih oštećenja. Ibogain inhibira ex vivo kontraktilnost ileuma u nivou doza ispod mM, pri čemu je efekat kod električne stimulacije, u odnosu na toničnu, značajno veći. Rezultati pretretmana na ileumu ex vivo ukazuju da ibogain svoje dejstvo ostvaruje delimično preko KATP kanala. Ibogain izaziva u ileumu ex vivo povećanje aktivnosti superoksid dismutaze (SOD) i katalaze (KAT), ali se ti efekti gube dodavanjem glibenklamida. Ibogain menja i redoks 7 ravnotežu u testisima, a nađeno povećanje aktivnosti SOD ukazuje na delikatnost očuvanja balansa koncentracija superoksida i vodonik peroksida koja se smatra krucijalnom u procesu kapacitacije spermatozoida. Rezultati pokazuju da ibogain u primenjenim dozama utiče na redoks status posmatranih tkiva i menja aktivnost antioksidativne odbrane, ali je odgovor visoko tkivno, dozno i vremenski specifičan, bez jasne zajedničke karakteristike., Ibogaine, administered as a single oral dose (1-25 mg/kg body weight, (b.w.)), has been used as an addiction-interrupting agent. Its effects persist for up to 72 h. Ex vivo results showed that ibogaine induced cellular energy consumption and restitution, followed by increased reactive oxygen species production and antioxidant activity. Therefore, the aim of this work was to explore the effect of a single oral dose of ibogaine (1 or 20 mg/kg b.w.) on antioxidative defenses in different rat tissues. Six and 24 h after ibogaine administration, histological examination showed glycogenolytic activity in hepatocytes, which was highest after 24 h in animals that received 20 mg/kg ibogaine. There were no changes in the activities of antioxidant enzymes in the liver and erythrocytes after ibogaine treatment, regardless of the dose. These results suggest there were no systemic ibogaine effects regarding antioxidant defense. Hepatic xanthine oxidase activity was elevated in rats that received 20 mg/kg compared to the controls, suggesting faster adenosine turnover. TBARS concentration was elevated in the group treated with 1 mg/kg after 24 h compared to the controls, suggesting mild oxidative stress. It was shown that ibogaine in heart after 6 h diminished the amount of protein -SH groups suggesting intra-protein oxidation, which along disturbed energetic homeostasis could lead to discrete cellular oxidative damages and possible heart failure. Ibogaine inhibited ex vivo ileum contractile activity with doses below mM. The effect was stronger in electrically stimulated contractions than tonic. Pretreatment with different potassium channel related agents showed that ibogaine obtained its inhibitory activity partially through KATP channels. Ibogaine induced the elevation of superoxide dismutase (SOD), catalase activity 9 ex vivo, but this effect was diminished by addition of glibenclamide. Ibogaine changed redox homeostasis in testicles as well, and elevated SOD activity suggested the importance of the preservation of delicate balance of superoxide and hydrogen peroxide concentrations that is crucial in sperm capacitation. Our results showed that ibogaine in doses applied in vivo per os influenced redox state and changed antioxidant activity, but response is highly tissue-, dose- and time- specific, without clear common characteristic.",
publisher = "Belgrade: Faculty of Medicine, University of Belgrade",
journal = "Faculty of Medicine, University of Belgrade",
title = "Efekat ibogaina na sistem antioksidativne zaštite u perifernim tkivima i kontraktilnost terminalnog ileuma kod mužjaka pacova, Effect of ibogaine on antioxidative defense system in peripheral tissues and terminal ileum contractility in male rats",
pages = "1-108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3473"
}

Vidonja Uzelac, T.. (2019). Efekat ibogaina na sistem antioksidativne zaštite u perifernim tkivima i kontraktilnost terminalnog ileuma kod mužjaka pacova. in Faculty of Medicine, University of Belgrade
Belgrade: Faculty of Medicine, University of Belgrade., 1-108.
https://hdl.handle.net/21.15107/rcub_ibiss_3473

Vidonja Uzelac T. Efekat ibogaina na sistem antioksidativne zaštite u perifernim tkivima i kontraktilnost terminalnog ileuma kod mužjaka pacova. in Faculty of Medicine, University of Belgrade. 2019;:1-108.
https://hdl.handle.net/21.15107/rcub_ibiss_3473 .

Vidonja Uzelac, Teodora, "Efekat ibogaina na sistem antioksidativne zaštite u perifernim tkivima i kontraktilnost terminalnog ileuma kod mužjaka pacova" in Faculty of Medicine, University of Belgrade (2019):1-108,
https://hdl.handle.net/21.15107/rcub_ibiss_3473 .

TY  - JOUR
AU  - Sokolović, Dragana
AU  - Drakul, Dragana
AU  - Oreščanin Dušić, Zorana
AU  - Tatalović, Nikola
AU  - Pecelj, Milica
AU  - Milovanović, Slobodan
AU  - Blagojević, Duško
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800031S
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3064
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3352
AB  - MgSO4 is used as a tocolytic agent. It is considered to be a calcium channel antagonist, but a different mechanism of its action might be involved. The aim of this study was to examine the contribution of calcium concentrations and potassium channels in the mechanism of MgSO4-mediated uterine relaxation. Isolated uteri from female Wister rats were treated with increasing MgSO4 concentrations (0.1-30 mM). MgSO4 induced dose-dependent inhibition of spontaneous activity. Addition of Ca2+ (6 mM and 12 mM) stimulated uterine contractile activity and attenuated the inhibitory activity of MgSO4. In order to analyze the role of different subtypes of potassium channels, Ca2+-stimulated uteri were pretreated with glibenclamide (Glib), a selective ATP-sensitive potassium channel inhibitor (KATP), tetraethylammonium (TEA), a non-specific inhibitor of large conductance calcium-activated potassium channels (BKCa), and 4-aminopyridine (4-AP), a voltage-sensitive potassium channel inhibitor (Kv), at concentrations that had no effect per se. Pretreatment with 4-AP had no effect on MgSO4-mediated relaxation of Ca2+-stimulated uteri. The relaxing effect of MgSO4 was potentiated by pretreatment with glibenclamide. Pretreatment with TEA attenuated the MgSO4-mediated decrease in frequency. Our results suggest that MgSO4 acts as a general calcium antagonist that influences Ca2+-mediated potassium channels. Furthermore, it seems that MgSO4 uterine relaxation activity is partially mediated by selective ATP-sensitive potassium channels, suggesting an ATP-dependent role.
T2  - Archives of Biological Sciences
T1  - The role of potassium channels and calcium in the relaxation mechanism of magnesium sulfate on the isolated rat uterus
IS  - 1
VL  - 71
DO  - 10.2298/ABS180615031S
SP  - 5
EP  - 11
ER  - 

@article{
author = "Sokolović, Dragana and Drakul, Dragana and Oreščanin Dušić, Zorana and Tatalović, Nikola and Pecelj, Milica and Milovanović, Slobodan and Blagojević, Duško",
year = "2019",
abstract = "MgSO4 is used as a tocolytic agent. It is considered to be a calcium channel antagonist, but a different mechanism of its action might be involved. The aim of this study was to examine the contribution of calcium concentrations and potassium channels in the mechanism of MgSO4-mediated uterine relaxation. Isolated uteri from female Wister rats were treated with increasing MgSO4 concentrations (0.1-30 mM). MgSO4 induced dose-dependent inhibition of spontaneous activity. Addition of Ca2+ (6 mM and 12 mM) stimulated uterine contractile activity and attenuated the inhibitory activity of MgSO4. In order to analyze the role of different subtypes of potassium channels, Ca2+-stimulated uteri were pretreated with glibenclamide (Glib), a selective ATP-sensitive potassium channel inhibitor (KATP), tetraethylammonium (TEA), a non-specific inhibitor of large conductance calcium-activated potassium channels (BKCa), and 4-aminopyridine (4-AP), a voltage-sensitive potassium channel inhibitor (Kv), at concentrations that had no effect per se. Pretreatment with 4-AP had no effect on MgSO4-mediated relaxation of Ca2+-stimulated uteri. The relaxing effect of MgSO4 was potentiated by pretreatment with glibenclamide. Pretreatment with TEA attenuated the MgSO4-mediated decrease in frequency. Our results suggest that MgSO4 acts as a general calcium antagonist that influences Ca2+-mediated potassium channels. Furthermore, it seems that MgSO4 uterine relaxation activity is partially mediated by selective ATP-sensitive potassium channels, suggesting an ATP-dependent role.",
journal = "Archives of Biological Sciences",
title = "The role of potassium channels and calcium in the relaxation mechanism of magnesium sulfate on the isolated rat uterus",
number = "1",
volume = "71",
doi = "10.2298/ABS180615031S",
pages = "5-11"
}

Sokolović, D., Drakul, D., Oreščanin Dušić, Z., Tatalović, N., Pecelj, M., Milovanović, S.,& Blagojević, D.. (2019). The role of potassium channels and calcium in the relaxation mechanism of magnesium sulfate on the isolated rat uterus. in Archives of Biological Sciences, 71(1), 5-11.
https://doi.org/10.2298/ABS180615031S

Sokolović D, Drakul D, Oreščanin Dušić Z, Tatalović N, Pecelj M, Milovanović S, Blagojević D. The role of potassium channels and calcium in the relaxation mechanism of magnesium sulfate on the isolated rat uterus. in Archives of Biological Sciences. 2019;71(1):5-11.
doi:10.2298/ABS180615031S .

Sokolović, Dragana, Drakul, Dragana, Oreščanin Dušić, Zorana, Tatalović, Nikola, Pecelj, Milica, Milovanović, Slobodan, Blagojević, Duško, "The role of potassium channels and calcium in the relaxation mechanism of magnesium sulfate on the isolated rat uterus" in Archives of Biological Sciences, 71, no. 1 (2019):5-11,
https://doi.org/10.2298/ABS180615031S . .

TY  - JOUR
AU  - Vidonja Uzelac, Teodora
AU  - Tatalović, Nikola
AU  - Mijović, Milica
AU  - Koželj, Gordana
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Bresjanac, Mara
AU  - Blagojević, Duško
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800055V
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3420
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3353
AB  - Ibogaine, administered as a single oral dose (1-25 mg/kg body weight), has been used as an addiction-interrupting agent. Its effects persist for up to 72 h. Ex vivo results showed that ibogaine induced cellular energy consumption and restitution, followed by increased reactive oxygen species production and antioxidant activity. Therefore, the aim of this work was to explore the effect of a single oral dose of ibogaine (1 or 20 mg/kg body weight) on antioxidative defenses in rat liver and erythrocytes. Six and 24 h after ibogaine administration, histological examination showed glycogenolytic activity in hepatocytes, which was highest after 24 h in animals that received 20 mg/kg ibogaine. There were no changes in the activities of superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase in the liver and erythrocytes after ibogaine treatment, regardless of the dose. Hepatic xanthine oxidase activity was elevated in rats that received 20 mg/kg compared to the controls (p<0.01), suggesting faster adenosine turnover. TBARS concentration was elevated in the group treated with 1 mg/kg after 24 h compared to the controls (p<0.01), suggesting mild oxidative stress. Our results show that ibogaine treatment influenced hepatic redox homeostasis, but not sufficiently to remodel antioxidant enzyme activities at 6 and 24 h post-ibogaine application.
T2  - Archives of Biological Sciences
T1  - Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes
IS  - 1
VL  - 71
DO  - 10.2298/ABS180918055V
SP  - 133
EP  - 144
ER  - 

@article{
author = "Vidonja Uzelac, Teodora and Tatalović, Nikola and Mijović, Milica and Koželj, Gordana and Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Bresjanac, Mara and Blagojević, Duško",
year = "2019",
abstract = "Ibogaine, administered as a single oral dose (1-25 mg/kg body weight), has been used as an addiction-interrupting agent. Its effects persist for up to 72 h. Ex vivo results showed that ibogaine induced cellular energy consumption and restitution, followed by increased reactive oxygen species production and antioxidant activity. Therefore, the aim of this work was to explore the effect of a single oral dose of ibogaine (1 or 20 mg/kg body weight) on antioxidative defenses in rat liver and erythrocytes. Six and 24 h after ibogaine administration, histological examination showed glycogenolytic activity in hepatocytes, which was highest after 24 h in animals that received 20 mg/kg ibogaine. There were no changes in the activities of superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase in the liver and erythrocytes after ibogaine treatment, regardless of the dose. Hepatic xanthine oxidase activity was elevated in rats that received 20 mg/kg compared to the controls (p<0.01), suggesting faster adenosine turnover. TBARS concentration was elevated in the group treated with 1 mg/kg after 24 h compared to the controls (p<0.01), suggesting mild oxidative stress. Our results show that ibogaine treatment influenced hepatic redox homeostasis, but not sufficiently to remodel antioxidant enzyme activities at 6 and 24 h post-ibogaine application.",
journal = "Archives of Biological Sciences",
title = "Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes",
number = "1",
volume = "71",
doi = "10.2298/ABS180918055V",
pages = "133-144"
}

Vidonja Uzelac, T., Tatalović, N., Mijović, M., Koželj, G., Nikolić-Kokić, A., Oreščanin Dušić, Z., Bresjanac, M.,& Blagojević, D.. (2019). Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes. in Archives of Biological Sciences, 71(1), 133-144.
https://doi.org/10.2298/ABS180918055V

Vidonja Uzelac T, Tatalović N, Mijović M, Koželj G, Nikolić-Kokić A, Oreščanin Dušić Z, Bresjanac M, Blagojević D. Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes. in Archives of Biological Sciences. 2019;71(1):133-144.
doi:10.2298/ABS180918055V .

Vidonja Uzelac, Teodora, Tatalović, Nikola, Mijović, Milica, Koželj, Gordana, Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Bresjanac, Mara, Blagojević, Duško, "Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes" in Archives of Biological Sciences, 71, no. 1 (2019):133-144,
https://doi.org/10.2298/ABS180918055V . .

TY  - JOUR
AU  - Nikolić, Tatjana
AU  - Kojić, Danijela
AU  - Orčić, Snežana
AU  - Vukašinović, Elvira
AU  - Blagojević, Duško
AU  - Purać, Jelena
PY  - 2019
UR  - http://link.springer.com/10.1007/s11356-018-3950-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3236
AB  - In the present study, the influence of cadmium, copper, and lead on two enzymes often used as biomarkers in toxicological analysis was investigated. Bees were fed with 1 M sucrose solution containing 10-fold serial dilutions of CuCl2 (1000 mg L-1, 100 mg L-1, and 10 mg L-1), CdCl2 (0.1 mg L-1, 0.01 mg L-1, and 0.001 mg L-1), or PbCl2 (10 mg L-1, 1 mg L-1, and 0.1 mg L-1) during 48 h. Our results showed that the total glutathione S-transferase activity was not changed under the influence of cadmium and lead, and it was decreased with the highest concentration of copper. The level of gene expression of the three analyzed classes of glutathione S-transferase was significantly increased with increasing concentrations of copper and cadmium. Lead did not cause significant changes in glutathione S-transferase activity and gene expression, while it showed biphasic effect on acetylcholinesterase activity: lower concentration of lead, 0.1 mg L-1 inhibited and higher dose, 10 mg L-1 induced acetylcholinesterase activity in honey bees. Furthermore, our results showed a significant decrease of the acetylcholinesterase activity in honey bees treated with 0.001 and 0.01 mg L-1 CdCl2. Our results indicate the influence of cadmium, copper, and lead on GST and AChE in the honey bees. These results form the basis for future research on the impact of metallic trace element pollution on honey bees.
T2  - Environmental Science and Pollution Research
T1  - Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.
DO  - 10.1007/s11356-018-3950-6
ER  - 

@article{
author = "Nikolić, Tatjana and Kojić, Danijela and Orčić, Snežana and Vukašinović, Elvira and Blagojević, Duško and Purać, Jelena",
year = "2019",
abstract = "In the present study, the influence of cadmium, copper, and lead on two enzymes often used as biomarkers in toxicological analysis was investigated. Bees were fed with 1 M sucrose solution containing 10-fold serial dilutions of CuCl2 (1000 mg L-1, 100 mg L-1, and 10 mg L-1), CdCl2 (0.1 mg L-1, 0.01 mg L-1, and 0.001 mg L-1), or PbCl2 (10 mg L-1, 1 mg L-1, and 0.1 mg L-1) during 48 h. Our results showed that the total glutathione S-transferase activity was not changed under the influence of cadmium and lead, and it was decreased with the highest concentration of copper. The level of gene expression of the three analyzed classes of glutathione S-transferase was significantly increased with increasing concentrations of copper and cadmium. Lead did not cause significant changes in glutathione S-transferase activity and gene expression, while it showed biphasic effect on acetylcholinesterase activity: lower concentration of lead, 0.1 mg L-1 inhibited and higher dose, 10 mg L-1 induced acetylcholinesterase activity in honey bees. Furthermore, our results showed a significant decrease of the acetylcholinesterase activity in honey bees treated with 0.001 and 0.01 mg L-1 CdCl2. Our results indicate the influence of cadmium, copper, and lead on GST and AChE in the honey bees. These results form the basis for future research on the impact of metallic trace element pollution on honey bees.",
journal = "Environmental Science and Pollution Research",
title = "Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.",
doi = "10.1007/s11356-018-3950-6"
}

Nikolić, T., Kojić, D., Orčić, S., Vukašinović, E., Blagojević, D.,& Purać, J.. (2019). Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.. in Environmental Science and Pollution Research.
https://doi.org/10.1007/s11356-018-3950-6

Nikolić T, Kojić D, Orčić S, Vukašinović E, Blagojević D, Purać J. Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.. in Environmental Science and Pollution Research. 2019;.
doi:10.1007/s11356-018-3950-6 .

Nikolić, Tatjana, Kojić, Danijela, Orčić, Snežana, Vukašinović, Elvira, Blagojević, Duško, Purać, Jelena, "Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead." in Environmental Science and Pollution Research (2019),
https://doi.org/10.1007/s11356-018-3950-6 . .

TY  - JOUR
AU  - Purać, Jelena
AU  - Nikolić, Tatjana V.
AU  - Kojić, Danijela
AU  - Ćelić, Anđelka S.
AU  - Plavša, Jovana J.
AU  - Blagojević, Duško
AU  - Petri, Edward T.
PY  - 2019
UR  - http://doi.wiley.com/10.1111/mec.14984
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3279
AB  - Metallothioneins are ubiquitous proteins important in metal homeostasis and detoxification. However, they have not previously been identified in honey bees or other Hymenoptera, where metallothioneins could be of ecophysiological and ecotoxicological significance. Better understanding of the molecular responses to stress induced by toxic metals could contribute to honey bee conservation. In addition, honey bee metallothionein could represent a biomarker for monitoring environmental quality. Here we identify and characterize a metallothionein gene in Apis mellifera (AmMT). AmMT is 1,680 bp long and encodes a 48 amino acids protein with 15 cysteines and no aromatic residues. A metal response element upstream of the start codon, coupled with numerous cis-regulatory elements indicate the functional context of AmMT. Molecular modelling predicts several transition metal binding sites, and comparative phylogenetic analysis revealed five putative metallothionein proteins in three other hymenoptera species. AmMT was characterized by cloning the full-length coding sequence of the putative metallothionein. Recombinant AmMT was found to increase metal tolerance upon overexpression in Escherichia coli supplemented with Cd, Cu or Pb. Finally, in laboratory tests on honey bees, gene expression profiles showed a dose-dependant relationship between Cd, Cu and Pb concentrations present in food and AmMT expression, while field experiments showed induction of AmMT in bees from an industrial site compared to those from an urban area. These studies suggest that AmMT has metal binding properties in agreement with a possible role in metal homeostasis. Further functional and structural characterization of metallothionein in honey bees and other Hymenoptera are necessary.
T2  - Molecular Ecology
T1  - Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.
IS  - 4
VL  - 28
DO  - 10.1111/mec.14984
SP  - 731
EP  - 745
ER  - 

@article{
author = "Purać, Jelena and Nikolić, Tatjana V. and Kojić, Danijela and Ćelić, Anđelka S. and Plavša, Jovana J. and Blagojević, Duško and Petri, Edward T.",
year = "2019",
abstract = "Metallothioneins are ubiquitous proteins important in metal homeostasis and detoxification. However, they have not previously been identified in honey bees or other Hymenoptera, where metallothioneins could be of ecophysiological and ecotoxicological significance. Better understanding of the molecular responses to stress induced by toxic metals could contribute to honey bee conservation. In addition, honey bee metallothionein could represent a biomarker for monitoring environmental quality. Here we identify and characterize a metallothionein gene in Apis mellifera (AmMT). AmMT is 1,680 bp long and encodes a 48 amino acids protein with 15 cysteines and no aromatic residues. A metal response element upstream of the start codon, coupled with numerous cis-regulatory elements indicate the functional context of AmMT. Molecular modelling predicts several transition metal binding sites, and comparative phylogenetic analysis revealed five putative metallothionein proteins in three other hymenoptera species. AmMT was characterized by cloning the full-length coding sequence of the putative metallothionein. Recombinant AmMT was found to increase metal tolerance upon overexpression in Escherichia coli supplemented with Cd, Cu or Pb. Finally, in laboratory tests on honey bees, gene expression profiles showed a dose-dependant relationship between Cd, Cu and Pb concentrations present in food and AmMT expression, while field experiments showed induction of AmMT in bees from an industrial site compared to those from an urban area. These studies suggest that AmMT has metal binding properties in agreement with a possible role in metal homeostasis. Further functional and structural characterization of metallothionein in honey bees and other Hymenoptera are necessary.",
journal = "Molecular Ecology",
title = "Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.",
number = "4",
volume = "28",
doi = "10.1111/mec.14984",
pages = "731-745"
}

Purać, J., Nikolić, T. V., Kojić, D., Ćelić, A. S., Plavša, J. J., Blagojević, D.,& Petri, E. T.. (2019). Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.. in Molecular Ecology, 28(4), 731-745.
https://doi.org/10.1111/mec.14984

Purać J, Nikolić TV, Kojić D, Ćelić AS, Plavša JJ, Blagojević D, Petri ET. Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.. in Molecular Ecology. 2019;28(4):731-745.
doi:10.1111/mec.14984 .

Purać, Jelena, Nikolić, Tatjana V., Kojić, Danijela, Ćelić, Anđelka S., Plavša, Jovana J., Blagojević, Duško, Petri, Edward T., "Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure." in Molecular Ecology, 28, no. 4 (2019):731-745,
https://doi.org/10.1111/mec.14984 . .

TY  - JOUR
AU  - Kojić, Danijela K.
AU  - Purać, Jelena S.
AU  - Nikolić, Tatjana V.
AU  - Orčić, Snežana M.
AU  - Vujanović, Dragana
AU  - Ilijević, Konstantin
AU  - Vukašinović, Elvira L.
AU  - Blagojević, Duško
PY  - 2019
UR  - http://www.schweizerbart.de/papers/entomologia/detail/39/91600/Oxidative_stress_and_the_activity_of_antioxidative?af=crossref
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3453
AB  - Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced expression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity.
T2  - Entomologia Generalis
T1  - Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees
IS  - 1
VL  - 39
DO  - 10.1127/entomologia/2019/0743
SP  - 33
EP  - 44
ER  - 

@article{
author = "Kojić, Danijela K. and Purać, Jelena S. and Nikolić, Tatjana V. and Orčić, Snežana M. and Vujanović, Dragana and Ilijević, Konstantin and Vukašinović, Elvira L. and Blagojević, Duško",
year = "2019",
abstract = "Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced expression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity.",
journal = "Entomologia Generalis",
title = "Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees",
number = "1",
volume = "39",
doi = "10.1127/entomologia/2019/0743",
pages = "33-44"
}

Kojić, D. K., Purać, J. S., Nikolić, T. V., Orčić, S. M., Vujanović, D., Ilijević, K., Vukašinović, E. L.,& Blagojević, D.. (2019). Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees. in Entomologia Generalis, 39(1), 33-44.
https://doi.org/10.1127/entomologia/2019/0743

Kojić DK, Purać JS, Nikolić TV, Orčić SM, Vujanović D, Ilijević K, Vukašinović EL, Blagojević D. Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees. in Entomologia Generalis. 2019;39(1):33-44.
doi:10.1127/entomologia/2019/0743 .

Kojić, Danijela K., Purać, Jelena S., Nikolić, Tatjana V., Orčić, Snežana M., Vujanović, Dragana, Ilijević, Konstantin, Vukašinović, Elvira L., Blagojević, Duško, "Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees" in Entomologia Generalis, 39, no. 1 (2019):33-44,
https://doi.org/10.1127/entomologia/2019/0743 . .

TY  - CONF
AU  - Savić, Danijela
AU  - Opačić, Miloš
AU  - Ristić, Aleksandar
AU  - Sokić, Dragoslav
AU  - Baščarević, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasić, Snežana
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5887
AB  - The accumulating evidence on the relation between the disturbed metal homeostasis
and epilepsy urges the need for data regarding the total metal concentrations, as well
as metal distribution in the brain itself, in order to indicate where to direct the
potential therapy, to metal supplementation or chelation. This paper summarizes our
results on the measurements of some important essential metals in hippocampi of
patients with mesial temporal lobe epilepsy (mTLE) who underwent
amigdalohippocampectomy. The key findings point out that levels of copper and
manganese are deficient in hippocampi of mTLE patients, and that their
concentrations correlated positively with neuronal loss in affected regions of sclerotic
hippocampus. In addition, the Cu concentration was decreased in the areas of total
neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor
deficient compared to control. Our results contribute to deeper insight of metals
biology in the epilepsy and may represent the initial point of new and non-invasive
therapy of drug resistant epilepsy.
PB  - Belgrade: Faculty of Chemistry: Serbian Biochemical Society
C3  - Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
T1  - Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy
SP  - 105
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5887
ER  - 

@conference{
author = "Savić, Danijela and Opačić, Miloš and Ristić, Aleksandar and Sokić, Dragoslav and Baščarević, Vladimir and Raičević, Savo and Savić, Slobodan and Živin, Marko and Šelih, Vid Simon and Spasić, Snežana and Spasojević, Ivan",
year = "2018",
abstract = "The accumulating evidence on the relation between the disturbed metal homeostasis
and epilepsy urges the need for data regarding the total metal concentrations, as well
as metal distribution in the brain itself, in order to indicate where to direct the
potential therapy, to metal supplementation or chelation. This paper summarizes our
results on the measurements of some important essential metals in hippocampi of
patients with mesial temporal lobe epilepsy (mTLE) who underwent
amigdalohippocampectomy. The key findings point out that levels of copper and
manganese are deficient in hippocampi of mTLE patients, and that their
concentrations correlated positively with neuronal loss in affected regions of sclerotic
hippocampus. In addition, the Cu concentration was decreased in the areas of total
neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor
deficient compared to control. Our results contribute to deeper insight of metals
biology in the epilepsy and may represent the initial point of new and non-invasive
therapy of drug resistant epilepsy.",
publisher = "Belgrade: Faculty of Chemistry: Serbian Biochemical Society",
journal = "Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia",
title = "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "105-112",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5887"
}

Savić, D., Opačić, M., Ristić, A., Sokić, D., Baščarević, V., Raičević, S., Savić, S., Živin, M., Šelih, V. S., Spasić, S.,& Spasojević, I.. (2018). Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
Belgrade: Faculty of Chemistry: Serbian Biochemical Society., 105-112.
https://hdl.handle.net/21.15107/rcub_ibiss_5887

Savić D, Opačić M, Ristić A, Sokić D, Baščarević V, Raičević S, Savić S, Živin M, Šelih VS, Spasić S, Spasojević I. Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia. 2018;:105-112.
https://hdl.handle.net/21.15107/rcub_ibiss_5887 .

Savić, Danijela, Opačić, Miloš, Ristić, Aleksandar, Sokić, Dragoslav, Baščarević, Vladimir, Raičević, Savo, Savić, Slobodan, Živin, Marko, Šelih, Vid Simon, Spasić, Snežana, Spasojević, Ivan, "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia (2018):105-112,
https://hdl.handle.net/21.15107/rcub_ibiss_5887 .

TY  - JOUR
AU  - Oreščanin Dušić, Zorana
AU  - Tatalović, Nikola
AU  - Vidonja Uzelac, Teodora
AU  - Brkljačić, Jelena
AU  - Nikolić-Kokić, Aleksandra
AU  - Mijušković, Ana
AU  - Spasić, Mihajlo
AU  - Paškulin, Roman
AU  - Bresjanac, Mara
AU  - Blagojević, Duško
PY  - 2018
UR  - https://www.hindawi.com/journals/omcl/2018/5969486/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2970
AB  - Ibogaine is an indole alkaloid originally extracted from the root bark of the African rainforest shrub Tabernanthe iboga. It has been explored as a treatment for substance abuse because it interrupts drug addiction and relieves withdrawal symptoms. However, it has been shown that ibogaine treatment leads to a sharp and transient fall in cellular ATP level followed by an increase of cellular respiration and ROS production. Since contractile tissues are sensitive to changes in the levels of ATP and ROS, here we investigated an ibogaine-mediated link between altered redox homeostasis and uterine contractile activity. We found that low concentrations of ibogaine stimulated contractile activity in spontaneously active uteri, but incremental increase of doses inhibited it. Inhibitory concentrations of ibogaine led to decreased SOD1 and elevated GSH-Px activity, but doses that completely inhibited contractions increased CAT activity. Western blot analyses showed that changes in enzyme activities were not due to elevated enzyme protein concentrations but posttranslational modifications. Changes in antioxidant enzyme activities point to a vast concentration-dependent increase in H2O2 level. Knowing that extracellular ATP stimulates isolated uterus contractility, while H2O2 has an inhibitory effect, this concentration-dependent stimulation/inhibition could be linked to ibogaine-related alterations in ATP level and redox homeostasis.
T2  - Oxidative Medicine and Cellular Longevity
T1  - The Effects of Ibogaine on Uterine Smooth Muscle Contractions: Relation to the Activity of Antioxidant Enzymes
VL  - 2018
DO  - 10.1155/2018/5969486
SP  - 5969486
ER  - 

@article{
author = "Oreščanin Dušić, Zorana and Tatalović, Nikola and Vidonja Uzelac, Teodora and Brkljačić, Jelena and Nikolić-Kokić, Aleksandra and Mijušković, Ana and Spasić, Mihajlo and Paškulin, Roman and Bresjanac, Mara and Blagojević, Duško",
year = "2018",
abstract = "Ibogaine is an indole alkaloid originally extracted from the root bark of the African rainforest shrub Tabernanthe iboga. It has been explored as a treatment for substance abuse because it interrupts drug addiction and relieves withdrawal symptoms. However, it has been shown that ibogaine treatment leads to a sharp and transient fall in cellular ATP level followed by an increase of cellular respiration and ROS production. Since contractile tissues are sensitive to changes in the levels of ATP and ROS, here we investigated an ibogaine-mediated link between altered redox homeostasis and uterine contractile activity. We found that low concentrations of ibogaine stimulated contractile activity in spontaneously active uteri, but incremental increase of doses inhibited it. Inhibitory concentrations of ibogaine led to decreased SOD1 and elevated GSH-Px activity, but doses that completely inhibited contractions increased CAT activity. Western blot analyses showed that changes in enzyme activities were not due to elevated enzyme protein concentrations but posttranslational modifications. Changes in antioxidant enzyme activities point to a vast concentration-dependent increase in H2O2 level. Knowing that extracellular ATP stimulates isolated uterus contractility, while H2O2 has an inhibitory effect, this concentration-dependent stimulation/inhibition could be linked to ibogaine-related alterations in ATP level and redox homeostasis.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "The Effects of Ibogaine on Uterine Smooth Muscle Contractions: Relation to the Activity of Antioxidant Enzymes",
volume = "2018",
doi = "10.1155/2018/5969486",
pages = "5969486"
}

Oreščanin Dušić, Z., Tatalović, N., Vidonja Uzelac, T., Brkljačić, J., Nikolić-Kokić, A., Mijušković, A., Spasić, M., Paškulin, R., Bresjanac, M.,& Blagojević, D.. (2018). The Effects of Ibogaine on Uterine Smooth Muscle Contractions: Relation to the Activity of Antioxidant Enzymes. in Oxidative Medicine and Cellular Longevity, 2018, 5969486.
https://doi.org/10.1155/2018/5969486

Oreščanin Dušić Z, Tatalović N, Vidonja Uzelac T, Brkljačić J, Nikolić-Kokić A, Mijušković A, Spasić M, Paškulin R, Bresjanac M, Blagojević D. The Effects of Ibogaine on Uterine Smooth Muscle Contractions: Relation to the Activity of Antioxidant Enzymes. in Oxidative Medicine and Cellular Longevity. 2018;2018:5969486.
doi:10.1155/2018/5969486 .

Oreščanin Dušić, Zorana, Tatalović, Nikola, Vidonja Uzelac, Teodora, Brkljačić, Jelena, Nikolić-Kokić, Aleksandra, Mijušković, Ana, Spasić, Mihajlo, Paškulin, Roman, Bresjanac, Mara, Blagojević, Duško, "The Effects of Ibogaine on Uterine Smooth Muscle Contractions: Relation to the Activity of Antioxidant Enzymes" in Oxidative Medicine and Cellular Longevity, 2018 (2018):5969486,
https://doi.org/10.1155/2018/5969486 . .

TY  - JOUR
AU  - Radić, Ivan
AU  - Nestorović, Vojkan
AU  - Mijović, Milica
AU  - Tatalović, Nikola
AU  - Joksimović, Bojan
AU  - Lukić, Vera
AU  - Mitić, Miloš
AU  - Adžić, Miroslav
AU  - Blagojević, Duško
AU  - Veličković, Stefan
AU  - Bulajić, Sonja
AU  - Đerković, Branislav
AU  - Mirić, Mirjana
AU  - Janićijević-Hudomal, Snežana
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800014R
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3282
AB  - We studied the effects of whey and pumpkin seed oil supplementation on the biochemical parameters in blood serum of male rats after chronic ad libitum alcohol consumption. The levels of AST, ALT, total bilirubin, ALP, LDH, triglycerides, total cholesterol, HDL, LDL, VLDL, triglyceride/HDL ratio, total cholesterol/HDL ratio (cholesterol ratio) and LDL/HDL ratio (index of atherosclerosis) were determined in rats after six weeks of treatment with: (i) ethanol (12% ethanol, ad libitum), (ii) whey (2 g/kg per day), (iii) pumpkin seed oil (2 mL/kg per day), (iv) both ethanol and whey, and (v) both ethanol and pumpkin seed oil. The results showed no changes in the levels of AST, ALT, total bilirubin, ALP, total cholesterol, HDL and VLDL in alcoholic rats when compared to the controls (fed with a standard laboratory diet ad libitum) and rats supplemented with whey and pumpkin seed oil. Our results suggest that alcohol consumption in small doses for 6 weeks changes lipid metabolism and significantly elevates the LDL/HDL ratio (index of atherosclerosis) but does not induce extensive liver damage. Ethanol consumption in our experimental conditions lowered the triglyceride level as well as the triglyceride/HDL ratio, suggesting lipid redistribution and the induction of some cardio-protective effect. However, ethanol induced a higher index of atherosclerosis. Pumpkin seed oil showed some protective potential in alcoholic rats by lowering the total cholesterol/HDL ratio, but it elevated the LDH. Whey consumption prevented elevation of the atherosclerosis index, pointing to its protective role, probably through the redistribution of lipids. However, whey in combination with ethanol elevated LDH.
T2  - Archives of Biological Sciences
T1  - The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol
IS  - 3
VL  - 70
DO  - 10.2298/ABS180320014R
SP  - 531
EP  - 541
ER  - 

@article{
author = "Radić, Ivan and Nestorović, Vojkan and Mijović, Milica and Tatalović, Nikola and Joksimović, Bojan and Lukić, Vera and Mitić, Miloš and Adžić, Miroslav and Blagojević, Duško and Veličković, Stefan and Bulajić, Sonja and Đerković, Branislav and Mirić, Mirjana and Janićijević-Hudomal, Snežana",
year = "2018",
abstract = "We studied the effects of whey and pumpkin seed oil supplementation on the biochemical parameters in blood serum of male rats after chronic ad libitum alcohol consumption. The levels of AST, ALT, total bilirubin, ALP, LDH, triglycerides, total cholesterol, HDL, LDL, VLDL, triglyceride/HDL ratio, total cholesterol/HDL ratio (cholesterol ratio) and LDL/HDL ratio (index of atherosclerosis) were determined in rats after six weeks of treatment with: (i) ethanol (12% ethanol, ad libitum), (ii) whey (2 g/kg per day), (iii) pumpkin seed oil (2 mL/kg per day), (iv) both ethanol and whey, and (v) both ethanol and pumpkin seed oil. The results showed no changes in the levels of AST, ALT, total bilirubin, ALP, total cholesterol, HDL and VLDL in alcoholic rats when compared to the controls (fed with a standard laboratory diet ad libitum) and rats supplemented with whey and pumpkin seed oil. Our results suggest that alcohol consumption in small doses for 6 weeks changes lipid metabolism and significantly elevates the LDL/HDL ratio (index of atherosclerosis) but does not induce extensive liver damage. Ethanol consumption in our experimental conditions lowered the triglyceride level as well as the triglyceride/HDL ratio, suggesting lipid redistribution and the induction of some cardio-protective effect. However, ethanol induced a higher index of atherosclerosis. Pumpkin seed oil showed some protective potential in alcoholic rats by lowering the total cholesterol/HDL ratio, but it elevated the LDH. Whey consumption prevented elevation of the atherosclerosis index, pointing to its protective role, probably through the redistribution of lipids. However, whey in combination with ethanol elevated LDH.",
journal = "Archives of Biological Sciences",
title = "The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol",
number = "3",
volume = "70",
doi = "10.2298/ABS180320014R",
pages = "531-541"
}

Radić, I., Nestorović, V., Mijović, M., Tatalović, N., Joksimović, B., Lukić, V., Mitić, M., Adžić, M., Blagojević, D., Veličković, S., Bulajić, S., Đerković, B., Mirić, M.,& Janićijević-Hudomal, S.. (2018). The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol. in Archives of Biological Sciences, 70(3), 531-541.
https://doi.org/10.2298/ABS180320014R

Radić I, Nestorović V, Mijović M, Tatalović N, Joksimović B, Lukić V, Mitić M, Adžić M, Blagojević D, Veličković S, Bulajić S, Đerković B, Mirić M, Janićijević-Hudomal S. The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol. in Archives of Biological Sciences. 2018;70(3):531-541.
doi:10.2298/ABS180320014R .

Radić, Ivan, Nestorović, Vojkan, Mijović, Milica, Tatalović, Nikola, Joksimović, Bojan, Lukić, Vera, Mitić, Miloš, Adžić, Miroslav, Blagojević, Duško, Veličković, Stefan, Bulajić, Sonja, Đerković, Branislav, Mirić, Mirjana, Janićijević-Hudomal, Snežana, "The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol" in Archives of Biological Sciences, 70, no. 3 (2018):531-541,
https://doi.org/10.2298/ABS180320014R . .

TY  - JOUR
AU  - Miljević, Čedo D.
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Milovanović, Maja
AU  - Munjiza, Ana
AU  - Jukić, Marin M
AU  - Pešić, Vesna
AU  - Lečić-Toševski, Dušica
AU  - Spasić, Mihajlo
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0165178117323272?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3154
AB  - To determine the relationship between alterations in the activity of the enzymes participating in antioxidative defense system and neurological soft signs (NSS) in schizophrenic patients with the first episode psychosis (SFE, n = 19), patients in relapse (SR, n = 46), and healthy controls (HC, n = 20). NSS intensity and enzymatic plasma activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) were compared between SFE, SR and HC subjects and a follow-up correlation analyses between the enzyme activities and NSS intensity was performed. NSS intensity was increased four times in schizophrenic patients compared with healthy controls. Activities of SOD and CAT were 40% decreased in SFE and these reductions were ameliorated by antipsychotic treatment. GPX activity was 20% decreased in both patient groups compared with controls. A negative correlation between NSS intensity and GPX activity was specifically found in the SFE patients. The data in this report argue that a reduction of GPX activity might be one of the causes for the emergence of NSS at the onset of schizophrenia, and provide the evidence that antipsychotic therapy can attenuate activity reductions of SOD and CAT, but not the activity reduction of GPX and the intensity of NSS.
T2  - Psychiatry research
T1  - Association between neurological soft signs and antioxidant enzyme activity in schizophrenic patients.
VL  - 269
DO  - 10.1016/j.psychres.2018.09.009
SP  - 746
EP  - 752
ER  - 

@article{
author = "Miljević, Čedo D. and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Milovanović, Maja and Munjiza, Ana and Jukić, Marin M and Pešić, Vesna and Lečić-Toševski, Dušica and Spasić, Mihajlo",
year = "2018",
abstract = "To determine the relationship between alterations in the activity of the enzymes participating in antioxidative defense system and neurological soft signs (NSS) in schizophrenic patients with the first episode psychosis (SFE, n = 19), patients in relapse (SR, n = 46), and healthy controls (HC, n = 20). NSS intensity and enzymatic plasma activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) were compared between SFE, SR and HC subjects and a follow-up correlation analyses between the enzyme activities and NSS intensity was performed. NSS intensity was increased four times in schizophrenic patients compared with healthy controls. Activities of SOD and CAT were 40% decreased in SFE and these reductions were ameliorated by antipsychotic treatment. GPX activity was 20% decreased in both patient groups compared with controls. A negative correlation between NSS intensity and GPX activity was specifically found in the SFE patients. The data in this report argue that a reduction of GPX activity might be one of the causes for the emergence of NSS at the onset of schizophrenia, and provide the evidence that antipsychotic therapy can attenuate activity reductions of SOD and CAT, but not the activity reduction of GPX and the intensity of NSS.",
journal = "Psychiatry research",
title = "Association between neurological soft signs and antioxidant enzyme activity in schizophrenic patients.",
volume = "269",
doi = "10.1016/j.psychres.2018.09.009",
pages = "746-752"
}

Miljević, Č. D., Nikolić-Kokić, A., Blagojević, D., Milovanović, M., Munjiza, A., Jukić, M. M., Pešić, V., Lečić-Toševski, D.,& Spasić, M.. (2018). Association between neurological soft signs and antioxidant enzyme activity in schizophrenic patients.. in Psychiatry research, 269, 746-752.
https://doi.org/10.1016/j.psychres.2018.09.009

Miljević ČD, Nikolić-Kokić A, Blagojević D, Milovanović M, Munjiza A, Jukić MM, Pešić V, Lečić-Toševski D, Spasić M. Association between neurological soft signs and antioxidant enzyme activity in schizophrenic patients.. in Psychiatry research. 2018;269:746-752.
doi:10.1016/j.psychres.2018.09.009 .

Miljević, Čedo D., Nikolić-Kokić, Aleksandra, Blagojević, Duško, Milovanović, Maja, Munjiza, Ana, Jukić, Marin M, Pešić, Vesna, Lečić-Toševski, Dušica, Spasić, Mihajlo, "Association between neurological soft signs and antioxidant enzyme activity in schizophrenic patients." in Psychiatry research, 269 (2018):746-752,
https://doi.org/10.1016/j.psychres.2018.09.009 . .

TY  - JOUR
AU  - Vukšinović, Elvira L.
AU  - Pond, David W.
AU  - Grubor-Lajšić, Gordana
AU  - Worland, M. Roger
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Popović, Željko D.
AU  - Blagojević, Duško
PY  - 2018
UR  - http://link.springer.com/10.1007/s00360-017-1110-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2777
AB  - Larvae of the European corn borer (Ostrinia nubilalis Hubn.) were cold acclimated during different phases of diapause to determine if changes in the fatty acid composition lipids occur as part of a programmed diapause strategy, or as a response to low temperatures during winter. Cold acclimation of fifth instar larvae of O. nubilalis during diapause had modest effects further on the readjustments in fatty acid composition of triacylglycerols and phospholipids. Overall, FA unsaturation (UFAs/SFAs ratio) was stable, with the exception of the triacylglycerols fraction after exposure to −3 and −10 °C in mid-diapause (MD) when it significantly increased. Differential scanning calorimetry (DSC) was used to examine phase transitions of total body lipid of cold-acclimated larvae in diapause. Thermal analysis indicated that changes in the melt transition temperatures of whole body total lipids were subtle, but consistent with the modest changes in the level of FA unsaturation observed. We conclude that lipid rearrangements are a function of the endogenous “diapause program” rather than a direct effect of low temperatures, which proved to have limited impact on lipid changes in diapausing larvae of O. nubilalis.
T2  - Journal of Comparative Physiology B
T1  - Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls
VL  - 188
DO  - 10.1007/s00360-017-1110-9
SP  - 27
EP  - 36
ER  - 

@article{
author = "Vukšinović, Elvira L. and Pond, David W. and Grubor-Lajšić, Gordana and Worland, M. Roger and Kojić, Danijela and Purać, Jelena and Popović, Željko D. and Blagojević, Duško",
year = "2018",
abstract = "Larvae of the European corn borer (Ostrinia nubilalis Hubn.) were cold acclimated during different phases of diapause to determine if changes in the fatty acid composition lipids occur as part of a programmed diapause strategy, or as a response to low temperatures during winter. Cold acclimation of fifth instar larvae of O. nubilalis during diapause had modest effects further on the readjustments in fatty acid composition of triacylglycerols and phospholipids. Overall, FA unsaturation (UFAs/SFAs ratio) was stable, with the exception of the triacylglycerols fraction after exposure to −3 and −10 °C in mid-diapause (MD) when it significantly increased. Differential scanning calorimetry (DSC) was used to examine phase transitions of total body lipid of cold-acclimated larvae in diapause. Thermal analysis indicated that changes in the melt transition temperatures of whole body total lipids were subtle, but consistent with the modest changes in the level of FA unsaturation observed. We conclude that lipid rearrangements are a function of the endogenous “diapause program” rather than a direct effect of low temperatures, which proved to have limited impact on lipid changes in diapausing larvae of O. nubilalis.",
journal = "Journal of Comparative Physiology B",
title = "Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls",
volume = "188",
doi = "10.1007/s00360-017-1110-9",
pages = "27-36"
}

Vukšinović, E. L., Pond, D. W., Grubor-Lajšić, G., Worland, M. R., Kojić, D., Purać, J., Popović, Ž. D.,& Blagojević, D.. (2018). Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls. in Journal of Comparative Physiology B, 188, 27-36.
https://doi.org/10.1007/s00360-017-1110-9

Vukšinović EL, Pond DW, Grubor-Lajšić G, Worland MR, Kojić D, Purać J, Popović ŽD, Blagojević D. Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls. in Journal of Comparative Physiology B. 2018;188:27-36.
doi:10.1007/s00360-017-1110-9 .

Vukšinović, Elvira L., Pond, David W., Grubor-Lajšić, Gordana, Worland, M. Roger, Kojić, Danijela, Purać, Jelena, Popović, Željko D., Blagojević, Duško, "Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls" in Journal of Comparative Physiology B, 188 (2018):27-36,
https://doi.org/10.1007/s00360-017-1110-9 . .

TY  - CONF
AU  - Guševac, Ivana
AU  - Tatalović, Nikola
AU  - Zarić, Marina
AU  - Martinović, Jelena
AU  - Mitrović, Nataša
AU  - Blagojević, Duško
AU  - Grković, Ivana
AU  - Drakulić, Dunja
PY  - 2018
UR  - https://forum2018.fens.org/
UR  - https://radar.ibiss.bg.ac.rs/123456789/3897
AB  - Potent neurosteroid progesterone (P4) strongly influences functional recovery in
various models of neurological diseases. However, its protective antioxidative
potential is not well established in stance of chronic cerebral hypoperfusion (CCH)
that induces ischemic milieu in the brain. CCH results in a cascade of events
leading to uncontrolled generation of 4-hydroxynonenal (HNE), a hallmark of
oxidative stress that can be abolished by mitochondrial antioxidant enzyme
manganese superoxide dismutase (MnSOD). Post-ischemic brain regeneration
requires preservation of cerebral blood flow that could be achieved by production of
vasodilatator nitric oxide (NO), synthesized by endothelial NO synthase (eNOS)
whose actions are regulated by phosphorylation at various sites. Decreased
phosphorylation of eNOS at Thr495 during antioxidative response, promotes its
activity and NO production, contributing to neuroprotection. Given the scarcity of
data about P4 antioxidative actions in prefrontal cortex during CCH, current study
assessed the level of HNE and NO, as well as MnSOD activity and the extent of
eNOS phosphorylation at Thr495 in rats subjected to sham operation and injected
with vehicle (commercial flax oil) or animals with permanent occlusion of common
carotid arteries and treated either with vehicle or P4 (1.7 mg/kg/day) for 7 days.
Results indicate that P4 decreases CCH-induced HNE generation and MnSOD
activity, diminishes inactivation of eNOS and increases production of protective NO.
In conclusion, P4 might downscale oxidative stress and boost antioxidant defense
capacity in CCH model, pointing investigated parameters as potential targets of P4
neuroprotective actions. Financially supported by MNTPR RS, grants 173044 and
41014.
PB  - Brussels, Belgium : Federation of European Neurocience Societies
C3  - 11th FENS Forum of Neuroscience
T1  - Progesterone downscales oxidative stress and boosts antioxidant defense capacity in rat model of permanent common carotid artery occlusion
SP  - 1971
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3897
ER  - 

@conference{
author = "Guševac, Ivana and Tatalović, Nikola and Zarić, Marina and Martinović, Jelena and Mitrović, Nataša and Blagojević, Duško and Grković, Ivana and Drakulić, Dunja",
year = "2018",
abstract = "Potent neurosteroid progesterone (P4) strongly influences functional recovery in
various models of neurological diseases. However, its protective antioxidative
potential is not well established in stance of chronic cerebral hypoperfusion (CCH)
that induces ischemic milieu in the brain. CCH results in a cascade of events
leading to uncontrolled generation of 4-hydroxynonenal (HNE), a hallmark of
oxidative stress that can be abolished by mitochondrial antioxidant enzyme
manganese superoxide dismutase (MnSOD). Post-ischemic brain regeneration
requires preservation of cerebral blood flow that could be achieved by production of
vasodilatator nitric oxide (NO), synthesized by endothelial NO synthase (eNOS)
whose actions are regulated by phosphorylation at various sites. Decreased
phosphorylation of eNOS at Thr495 during antioxidative response, promotes its
activity and NO production, contributing to neuroprotection. Given the scarcity of
data about P4 antioxidative actions in prefrontal cortex during CCH, current study
assessed the level of HNE and NO, as well as MnSOD activity and the extent of
eNOS phosphorylation at Thr495 in rats subjected to sham operation and injected
with vehicle (commercial flax oil) or animals with permanent occlusion of common
carotid arteries and treated either with vehicle or P4 (1.7 mg/kg/day) for 7 days.
Results indicate that P4 decreases CCH-induced HNE generation and MnSOD
activity, diminishes inactivation of eNOS and increases production of protective NO.
In conclusion, P4 might downscale oxidative stress and boost antioxidant defense
capacity in CCH model, pointing investigated parameters as potential targets of P4
neuroprotective actions. Financially supported by MNTPR RS, grants 173044 and
41014.",
publisher = "Brussels, Belgium : Federation of European Neurocience Societies",
journal = "11th FENS Forum of Neuroscience",
title = "Progesterone downscales oxidative stress and boosts antioxidant defense capacity in rat model of permanent common carotid artery occlusion",
pages = "1971",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3897"
}

Guševac, I., Tatalović, N., Zarić, M., Martinović, J., Mitrović, N., Blagojević, D., Grković, I.,& Drakulić, D.. (2018). Progesterone downscales oxidative stress and boosts antioxidant defense capacity in rat model of permanent common carotid artery occlusion. in 11th FENS Forum of Neuroscience
Brussels, Belgium : Federation of European Neurocience Societies., 1971.
https://hdl.handle.net/21.15107/rcub_ibiss_3897

Guševac I, Tatalović N, Zarić M, Martinović J, Mitrović N, Blagojević D, Grković I, Drakulić D. Progesterone downscales oxidative stress and boosts antioxidant defense capacity in rat model of permanent common carotid artery occlusion. in 11th FENS Forum of Neuroscience. 2018;:1971.
https://hdl.handle.net/21.15107/rcub_ibiss_3897 .

Guševac, Ivana, Tatalović, Nikola, Zarić, Marina, Martinović, Jelena, Mitrović, Nataša, Blagojević, Duško, Grković, Ivana, Drakulić, Dunja, "Progesterone downscales oxidative stress and boosts antioxidant defense capacity in rat model of permanent common carotid artery occlusion" in 11th FENS Forum of Neuroscience (2018):1971,
https://hdl.handle.net/21.15107/rcub_ibiss_3897 .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Tatalović, Nikola
AU  - Brkljačić, Jelena
AU  - Mijović, Milica
AU  - Mijušković, Ana
AU  - Miler, Marko
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić, Milan
AU  - Milošević, Verica
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Miljević, Čedo
PY  - 2018
UR  - https://www.tandfonline.com/doi/full/10.1080/15287394.2018.1495587
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3121
AB  - Atypical antipsychotics produce severe side effects including myocarditis that may be attributed to oxidative stress. The aim of this study was to investigate the influence of clozapine, ziprasidone, and sertindole on rat heart morphology and determine whether redox imbalane plays a role in development of histopathological changes. Adult 3-month-old male Wistar rats were treated with recommended daily dose for selected drugs. After 4 week treatment histopathological analysis of the heart was performed and expression and activity of antioxidant enzymes determined. All examined drugs induced histopathological changes that were characterized as toxic myocarditis. Degenerative changes in cardiomyocytes were accompanied by lymphocytic infiltration as well as pericardial histopathological alterations in all treated groups. The least prominent changes were observed in sertindole-treated animals, and most severe with clozapine. Clozapine increased superoxide dismutase 1 (SOD1) activity while ziprasidone reduced glutathione reductase (GR) activity. Sertindole exerted no marked effect on antioxidant enzyme function in the heart even though myocardial degeneration was noted. In conclusion, treatment with clozapine or ziprasidone induced pathophysiological alterations in rat heart, which appeared to be associated disturbances in antioxidant capacity. Abbreviation: AAP, Atypical antipsychotics; ROS, reactive oxygen species; SOD1, Copper-zinc superoxide dismutase; SOD2, Manganese superoxide dismutase; CAT, Catalase; GPx, Glutathione peroxidase; GR, Glutathione reductase; H&E, hematoxylin and eosin stain; TNF- α, tumor necrosis factor alpha.
T2  - Journal of Toxicology and Environmental Health, Part A
T1  - Clozapine, ziprasidone, and sertindole-induced morphological changes in the rat heart and their relationship to antioxidant enzymes function
IS  - 17
VL  - 81
DO  - 10.1080/15287394.2018.1495587
SP  - 844
EP  - 853
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Tatalović, Nikola and Brkljačić, Jelena and Mijović, Milica and Mijušković, Ana and Miler, Marko and Oreščanin Dušić, Zorana and Nikolić, Milan and Milošević, Verica and Blagojević, Duško and Spasić, Mihajlo and Miljević, Čedo",
year = "2018",
abstract = "Atypical antipsychotics produce severe side effects including myocarditis that may be attributed to oxidative stress. The aim of this study was to investigate the influence of clozapine, ziprasidone, and sertindole on rat heart morphology and determine whether redox imbalane plays a role in development of histopathological changes. Adult 3-month-old male Wistar rats were treated with recommended daily dose for selected drugs. After 4 week treatment histopathological analysis of the heart was performed and expression and activity of antioxidant enzymes determined. All examined drugs induced histopathological changes that were characterized as toxic myocarditis. Degenerative changes in cardiomyocytes were accompanied by lymphocytic infiltration as well as pericardial histopathological alterations in all treated groups. The least prominent changes were observed in sertindole-treated animals, and most severe with clozapine. Clozapine increased superoxide dismutase 1 (SOD1) activity while ziprasidone reduced glutathione reductase (GR) activity. Sertindole exerted no marked effect on antioxidant enzyme function in the heart even though myocardial degeneration was noted. In conclusion, treatment with clozapine or ziprasidone induced pathophysiological alterations in rat heart, which appeared to be associated disturbances in antioxidant capacity. Abbreviation: AAP, Atypical antipsychotics; ROS, reactive oxygen species; SOD1, Copper-zinc superoxide dismutase; SOD2, Manganese superoxide dismutase; CAT, Catalase; GPx, Glutathione peroxidase; GR, Glutathione reductase; H&E, hematoxylin and eosin stain; TNF- α, tumor necrosis factor alpha.",
journal = "Journal of Toxicology and Environmental Health, Part A",
title = "Clozapine, ziprasidone, and sertindole-induced morphological changes in the rat heart and their relationship to antioxidant enzymes function",
number = "17",
volume = "81",
doi = "10.1080/15287394.2018.1495587",
pages = "844-853"
}

Nikolić-Kokić, A., Tatalović, N., Brkljačić, J., Mijović, M., Mijušković, A., Miler, M., Oreščanin Dušić, Z., Nikolić, M., Milošević, V., Blagojević, D., Spasić, M.,& Miljević, Č.. (2018). Clozapine, ziprasidone, and sertindole-induced morphological changes in the rat heart and their relationship to antioxidant enzymes function. in Journal of Toxicology and Environmental Health, Part A, 81(17), 844-853.
https://doi.org/10.1080/15287394.2018.1495587

Nikolić-Kokić A, Tatalović N, Brkljačić J, Mijović M, Mijušković A, Miler M, Oreščanin Dušić Z, Nikolić M, Milošević V, Blagojević D, Spasić M, Miljević Č. Clozapine, ziprasidone, and sertindole-induced morphological changes in the rat heart and their relationship to antioxidant enzymes function. in Journal of Toxicology and Environmental Health, Part A. 2018;81(17):844-853.
doi:10.1080/15287394.2018.1495587 .

Nikolić-Kokić, Aleksandra, Tatalović, Nikola, Brkljačić, Jelena, Mijović, Milica, Mijušković, Ana, Miler, Marko, Oreščanin Dušić, Zorana, Nikolić, Milan, Milošević, Verica, Blagojević, Duško, Spasić, Mihajlo, Miljević, Čedo, "Clozapine, ziprasidone, and sertindole-induced morphological changes in the rat heart and their relationship to antioxidant enzymes function" in Journal of Toxicology and Environmental Health, Part A, 81, no. 17 (2018):844-853,
https://doi.org/10.1080/15287394.2018.1495587 . .

TY  - JOUR
AU  - Kojić, Danijela
AU  - Popović, Željko D.
AU  - Orčić, Dejan
AU  - Purać, Jelena
AU  - Orčić, Snežana
AU  - Vukašinović, Elvira L.
AU  - Nikolić, Tatjana V.
AU  - Blagojević, Duško
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0022191018300921?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3116
AB  - The European corn borer (ECB, Ostrinia nubilalis Hbn.) is a major pest in temperate regions of Europe and North America. Fifth instar ECB larvae enter diapause before winter and gradually develop cold hardiness. Here we investigated the combined influence of diapause phase and low temperature on sugar and polyol content in ECB larvae. Larvae in mid-diapause or diapause termination were acclimated at 5 °C, -3°C or -16 °C, and sugar and polyol content was measured using GC-MS. Control GC-MS measurements were conducted on untreated non-diapausing larvae. We detected differences in polyol (glycerol, sorbitol, myo-inositol) and sugar (trehalose, fructose, glucose) levels in diapausing versus non-diapausing larvae. Glycerol and trehalose were the most abundant of all analyzed cryoprotective compounds in diapausing larvae. Exposure of diapausing larvae to decreasing temperatures induced changes in polyol and sugar levels that depended on the phase of diapause. In mid-diapause larvae, decreasing temperatures induced a significant increase in glycerol and a decrease in sorbitol and myo-inositol. In larvae at diapause termination, polyol content was lower and less influenced by decreasing temperatures. In contrast, sugar levels were lower in larvae at mid-diapause versus diapause termination. Exposure of larvae to -16 °C induced a significant increase in the levels of all detected sugars. In particular, glucose levels were significantly higher in larvae at diapause termination following exposure to -16 °C. We propose that this shift toward sugar synthesis following low temperature exposure in larvae at diapause termination is a consequence of NADPH dependent polyol synthesis, and may be a mechanism for preservation of carbon reserves needed for post-diapause development.
T2  - Journal of insect physiology
T1  - The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).
VL  - 109
DO  - 10.1016/j.jinsphys.2018.07.007
SP  - 107
EP  - 113
ER  - 

@article{
author = "Kojić, Danijela and Popović, Željko D. and Orčić, Dejan and Purać, Jelena and Orčić, Snežana and Vukašinović, Elvira L. and Nikolić, Tatjana V. and Blagojević, Duško",
year = "2018",
abstract = "The European corn borer (ECB, Ostrinia nubilalis Hbn.) is a major pest in temperate regions of Europe and North America. Fifth instar ECB larvae enter diapause before winter and gradually develop cold hardiness. Here we investigated the combined influence of diapause phase and low temperature on sugar and polyol content in ECB larvae. Larvae in mid-diapause or diapause termination were acclimated at 5 °C, -3°C or -16 °C, and sugar and polyol content was measured using GC-MS. Control GC-MS measurements were conducted on untreated non-diapausing larvae. We detected differences in polyol (glycerol, sorbitol, myo-inositol) and sugar (trehalose, fructose, glucose) levels in diapausing versus non-diapausing larvae. Glycerol and trehalose were the most abundant of all analyzed cryoprotective compounds in diapausing larvae. Exposure of diapausing larvae to decreasing temperatures induced changes in polyol and sugar levels that depended on the phase of diapause. In mid-diapause larvae, decreasing temperatures induced a significant increase in glycerol and a decrease in sorbitol and myo-inositol. In larvae at diapause termination, polyol content was lower and less influenced by decreasing temperatures. In contrast, sugar levels were lower in larvae at mid-diapause versus diapause termination. Exposure of larvae to -16 °C induced a significant increase in the levels of all detected sugars. In particular, glucose levels were significantly higher in larvae at diapause termination following exposure to -16 °C. We propose that this shift toward sugar synthesis following low temperature exposure in larvae at diapause termination is a consequence of NADPH dependent polyol synthesis, and may be a mechanism for preservation of carbon reserves needed for post-diapause development.",
journal = "Journal of insect physiology",
title = "The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).",
volume = "109",
doi = "10.1016/j.jinsphys.2018.07.007",
pages = "107-113"
}

Kojić, D., Popović, Ž. D., Orčić, D., Purać, J., Orčić, S., Vukašinović, E. L., Nikolić, T. V.,& Blagojević, D.. (2018). The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).. in Journal of insect physiology, 109, 107-113.
https://doi.org/10.1016/j.jinsphys.2018.07.007

Kojić D, Popović ŽD, Orčić D, Purać J, Orčić S, Vukašinović EL, Nikolić TV, Blagojević D. The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).. in Journal of insect physiology. 2018;109:107-113.
doi:10.1016/j.jinsphys.2018.07.007 .

Kojić, Danijela, Popović, Željko D., Orčić, Dejan, Purać, Jelena, Orčić, Snežana, Vukašinović, Elvira L., Nikolić, Tatjana V., Blagojević, Duško, "The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.)." in Journal of insect physiology, 109 (2018):107-113,
https://doi.org/10.1016/j.jinsphys.2018.07.007 . .

TY  - JOUR
AU  - Mitrović, Marko
AU  - Tatalović, Nikola
AU  - Nikolić-Kokić, Aleksandra
AU  - Ciraj-Bjelac, Olivera
AU  - Krstić, Nikola
AU  - Oreščanin Dušić, Zorana
AU  - Krstić, Dragana
AU  - Jovanović, Zoran
AU  - Blagojević, Duško
AU  - Lazarević-Macanović, Mirjana
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800029M
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2841
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3226
AB  - In recent years, computed tomography (CT) has become very common in veterinary medicine. It is well known that testicles are organs with high radiosensitivity and their function can be impaired even after exposure to low radiation doses. In this work, we calculated the absorbed radiation doses after CT was performed with different voltage/current levels and correlated it with the activity of antioxidant enzymes in rabbit testicles. Two hours after CT, the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) were increased in the testicles of animals that received an absorbed dose of 29.2 mGy. The same changes, along with elevated glutathione reductase (GR) activity, were observed after 7 days in animals that received the highest absorbed dose (46.3 mGy). It would appear that absorbed doses above 27.8 mGy provoked the antioxidant reaction but the time scale of the reaction was dose-dependent. Examination of the obtained results revealed that the main denominator of CT influence was a higher current. Our results suggest that CT influences the antioxidant status in rabbit testicles. The changes in antioxidant enzyme activities were dose- and time-dependent and influenced by the applied current.
T2  - Archives of Biological Sciences
T1  - Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles
IS  - 4
VL  - 70
DO  - 10.2298/ABS180413029M
SP  - 675
EP  - 680
ER  - 

@article{
author = "Mitrović, Marko and Tatalović, Nikola and Nikolić-Kokić, Aleksandra and Ciraj-Bjelac, Olivera and Krstić, Nikola and Oreščanin Dušić, Zorana and Krstić, Dragana and Jovanović, Zoran and Blagojević, Duško and Lazarević-Macanović, Mirjana",
year = "2018",
abstract = "In recent years, computed tomography (CT) has become very common in veterinary medicine. It is well known that testicles are organs with high radiosensitivity and their function can be impaired even after exposure to low radiation doses. In this work, we calculated the absorbed radiation doses after CT was performed with different voltage/current levels and correlated it with the activity of antioxidant enzymes in rabbit testicles. Two hours after CT, the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) were increased in the testicles of animals that received an absorbed dose of 29.2 mGy. The same changes, along with elevated glutathione reductase (GR) activity, were observed after 7 days in animals that received the highest absorbed dose (46.3 mGy). It would appear that absorbed doses above 27.8 mGy provoked the antioxidant reaction but the time scale of the reaction was dose-dependent. Examination of the obtained results revealed that the main denominator of CT influence was a higher current. Our results suggest that CT influences the antioxidant status in rabbit testicles. The changes in antioxidant enzyme activities were dose- and time-dependent and influenced by the applied current.",
journal = "Archives of Biological Sciences",
title = "Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles",
number = "4",
volume = "70",
doi = "10.2298/ABS180413029M",
pages = "675-680"
}

Mitrović, M., Tatalović, N., Nikolić-Kokić, A., Ciraj-Bjelac, O., Krstić, N., Oreščanin Dušić, Z., Krstić, D., Jovanović, Z., Blagojević, D.,& Lazarević-Macanović, M.. (2018). Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles. in Archives of Biological Sciences, 70(4), 675-680.
https://doi.org/10.2298/ABS180413029M

Mitrović M, Tatalović N, Nikolić-Kokić A, Ciraj-Bjelac O, Krstić N, Oreščanin Dušić Z, Krstić D, Jovanović Z, Blagojević D, Lazarević-Macanović M. Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles. in Archives of Biological Sciences. 2018;70(4):675-680.
doi:10.2298/ABS180413029M .

Mitrović, Marko, Tatalović, Nikola, Nikolić-Kokić, Aleksandra, Ciraj-Bjelac, Olivera, Krstić, Nikola, Oreščanin Dušić, Zorana, Krstić, Dragana, Jovanović, Zoran, Blagojević, Duško, Lazarević-Macanović, Mirjana, "Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles" in Archives of Biological Sciences, 70, no. 4 (2018):675-680,
https://doi.org/10.2298/ABS180413029M . .