TY  - JOUR
AU  - Bogojević, Desanka
AU  - Poznanović, Goran
AU  - Grdović, Nevena
AU  - Grigorov, Ilijana
AU  - Vidaković, Melita
AU  - Dinić, Svetlana
AU  - Mihailović, Mirjana
PY  - 2011
UR  - https://link.springer.com/article/10.1007%2Fs00411-010-0331-z
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3202
AB  - Previously, we showed that administration of the acute-phase protein α(2)-macroglobulin (α(2)M) to rats before total-body irradiation with 6.7 Gy (LD(50/30)) of X-rays provides the same level of radioprotection as amifostine. Here, we compare the cytoprotective effects of α(2)M and amifostine on rat liver. The potential of the liver to replenish cells destroyed by ionizing radiation was assessed by immunoblot analysis with antibody to proliferating cell nuclear antigen (PCNA). After irradiation, in unprotected rats PCNA decreased 6-fold from the basal level. In rats pretreated with either α(2)M or amifostine, PCNA was increased throughout a 4 week follow-up period, indicating that hepatocyte proliferation was unaffected. Since PCNA is an important component of the repair machinery, its increased expression was accompanied by significantly lower DNA damage in α(2)M- and amifostine-treated rats. At 2 weeks after irradiation, the Comet assay revealed a 15-fold increase in DNA damage in unprotected rats, while in α(2)M- and amifostine-treated rats we observed 3- and 4-fold rise in damage, respectively. The improved protection to DNA damage was supported by elevated activity of the antioxidant systems. Compared to untreated rats, pretreatments with α(2)M and amifostine led to similar increases in levels of the inflammatory cytokine IL-6 and the redox-sensitive transcription factor NFκB, promoting upregulation of MnSOD, the major component of the cell's antioxidant axis, and subsequent increases in Mn/CuZnSOD and catalase enzymatic activities. The results show that α(2)M induces protein factors whose interplay underlies radioprotection and support the idea that α(2)M is the central effector of natural radioprotection in the rat.
PB  - Springer Nature
T2  - Radiation and Environmental Biophysics
T1  - Administration of rat acute-phase protein α(2)-macroglobulin before total-body irradiation initiates cytoprotective mechanisms in the liver.
IS  - 1
VL  - 50
DO  - 10.1007/s00411-010-0331-z
SP  - 167
EP  - 179
ER  - 

@article{
author = "Bogojević, Desanka and Poznanović, Goran and Grdović, Nevena and Grigorov, Ilijana and Vidaković, Melita and Dinić, Svetlana and Mihailović, Mirjana",
year = "2011",
abstract = "Previously, we showed that administration of the acute-phase protein α(2)-macroglobulin (α(2)M) to rats before total-body irradiation with 6.7 Gy (LD(50/30)) of X-rays provides the same level of radioprotection as amifostine. Here, we compare the cytoprotective effects of α(2)M and amifostine on rat liver. The potential of the liver to replenish cells destroyed by ionizing radiation was assessed by immunoblot analysis with antibody to proliferating cell nuclear antigen (PCNA). After irradiation, in unprotected rats PCNA decreased 6-fold from the basal level. In rats pretreated with either α(2)M or amifostine, PCNA was increased throughout a 4 week follow-up period, indicating that hepatocyte proliferation was unaffected. Since PCNA is an important component of the repair machinery, its increased expression was accompanied by significantly lower DNA damage in α(2)M- and amifostine-treated rats. At 2 weeks after irradiation, the Comet assay revealed a 15-fold increase in DNA damage in unprotected rats, while in α(2)M- and amifostine-treated rats we observed 3- and 4-fold rise in damage, respectively. The improved protection to DNA damage was supported by elevated activity of the antioxidant systems. Compared to untreated rats, pretreatments with α(2)M and amifostine led to similar increases in levels of the inflammatory cytokine IL-6 and the redox-sensitive transcription factor NFκB, promoting upregulation of MnSOD, the major component of the cell's antioxidant axis, and subsequent increases in Mn/CuZnSOD and catalase enzymatic activities. The results show that α(2)M induces protein factors whose interplay underlies radioprotection and support the idea that α(2)M is the central effector of natural radioprotection in the rat.",
publisher = "Springer Nature",
journal = "Radiation and Environmental Biophysics",
title = "Administration of rat acute-phase protein α(2)-macroglobulin before total-body irradiation initiates cytoprotective mechanisms in the liver.",
number = "1",
volume = "50",
doi = "10.1007/s00411-010-0331-z",
pages = "167-179"
}

Bogojević, D., Poznanović, G., Grdović, N., Grigorov, I., Vidaković, M., Dinić, S.,& Mihailović, M.. (2011). Administration of rat acute-phase protein α(2)-macroglobulin before total-body irradiation initiates cytoprotective mechanisms in the liver.. in Radiation and Environmental Biophysics
Springer Nature., 50(1), 167-179.
https://doi.org/10.1007/s00411-010-0331-z

Bogojević D, Poznanović G, Grdović N, Grigorov I, Vidaković M, Dinić S, Mihailović M. Administration of rat acute-phase protein α(2)-macroglobulin before total-body irradiation initiates cytoprotective mechanisms in the liver.. in Radiation and Environmental Biophysics. 2011;50(1):167-179.
doi:10.1007/s00411-010-0331-z .

Bogojević, Desanka, Poznanović, Goran, Grdović, Nevena, Grigorov, Ilijana, Vidaković, Melita, Dinić, Svetlana, Mihailović, Mirjana, "Administration of rat acute-phase protein α(2)-macroglobulin before total-body irradiation initiates cytoprotective mechanisms in the liver." in Radiation and Environmental Biophysics, 50, no. 1 (2011):167-179,
https://doi.org/10.1007/s00411-010-0331-z . .

TY  - JOUR
AU  - Mujić, Aida
AU  - Grdović, Nevena
AU  - Mujić, Ibrahim
AU  - Mihailović, Mirjana
AU  - Živković, Jelena
AU  - Poznanović, Goran
AU  - Vidaković, Melita
PY  - 2011
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-78149407400&partnerID=tZOtx3y1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3203
AB  - Oxidative stress plays a central role in the onset of diabetes which is characterised by progressive pancreatic β-cell failure. We studied the ability of phenolic extracts prepared from chestnut leaf, catkin and spiny burs to prevent oxidative stress-induced β-cell (Rin-5F) death. The Rin-5F cell death was induced by treatment with the diabetogenic agent streptozotocin (STZ) and the protective effects of the chestnut extracts were assessed after cells treatment with a STZ/extracts mixture. The chestnut extracts increased cell viability by protecting DNA from oxidative damage and by enhancing the natural antioxidant system. Moreover, the chestnut extracts had a significant impact on the cell's redox-status which was observed as an increase in the levels of reduced glutathione. Chestnut extracts were capable of inhibiting lipid peroxidation, which was manifested as a lowering of malondialdehyde levels. We concluded that chestnut extracts increased cell viability after STZ treatment as a result of their antioxidative properties. © 2010 Elsevier Ltd.
PB  - Elsevier
T2  - Food Chemistry
T1  - Antioxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic β-cells
IS  - 3
VL  - 125
DO  - 10.1016/j.foodchem.2010.08.068
SP  - 841
EP  - 849
ER  - 

@article{
author = "Mujić, Aida and Grdović, Nevena and Mujić, Ibrahim and Mihailović, Mirjana and Živković, Jelena and Poznanović, Goran and Vidaković, Melita",
year = "2011",
abstract = "Oxidative stress plays a central role in the onset of diabetes which is characterised by progressive pancreatic β-cell failure. We studied the ability of phenolic extracts prepared from chestnut leaf, catkin and spiny burs to prevent oxidative stress-induced β-cell (Rin-5F) death. The Rin-5F cell death was induced by treatment with the diabetogenic agent streptozotocin (STZ) and the protective effects of the chestnut extracts were assessed after cells treatment with a STZ/extracts mixture. The chestnut extracts increased cell viability by protecting DNA from oxidative damage and by enhancing the natural antioxidant system. Moreover, the chestnut extracts had a significant impact on the cell's redox-status which was observed as an increase in the levels of reduced glutathione. Chestnut extracts were capable of inhibiting lipid peroxidation, which was manifested as a lowering of malondialdehyde levels. We concluded that chestnut extracts increased cell viability after STZ treatment as a result of their antioxidative properties. © 2010 Elsevier Ltd.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Antioxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic β-cells",
number = "3",
volume = "125",
doi = "10.1016/j.foodchem.2010.08.068",
pages = "841-849"
}

Mujić, A., Grdović, N., Mujić, I., Mihailović, M., Živković, J., Poznanović, G.,& Vidaković, M.. (2011). Antioxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic β-cells. in Food Chemistry
Elsevier., 125(3), 841-849.
https://doi.org/10.1016/j.foodchem.2010.08.068

Mujić A, Grdović N, Mujić I, Mihailović M, Živković J, Poznanović G, Vidaković M. Antioxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic β-cells. in Food Chemistry. 2011;125(3):841-849.
doi:10.1016/j.foodchem.2010.08.068 .

Mujić, Aida, Grdović, Nevena, Mujić, Ibrahim, Mihailović, Mirjana, Živković, Jelena, Poznanović, Goran, Vidaković, Melita, "Antioxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic β-cells" in Food Chemistry, 125, no. 3 (2011):841-849,
https://doi.org/10.1016/j.foodchem.2010.08.068 . .

TY  - CONF
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
AU  - Trifunović, Svetlana
AU  - Bogojević, Desanka
AU  - Ivanović-Matić, Svetlana
AU  - Martinović, Vesna
AU  - Grigorov, Ilijana
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6100
AB  - Dietary restriction (DR) in female rats pre­ sumably activates the stress (hypothalamo-pituitary­ adrenal) system. It's well known that various pituitary cell types are involved in the reaction to stress, i.e. adrenocorticotropic hormone (ACTH), prolactin and growth hormone cells. The aim of this study was to examine the effects of DR on the immunohistomor­ phometric features of pituitary ACTH cells and blood levels of ACTH. Peripubertal (38 days old) female Wistar rats were housed one per cage, under standard environmental conditions (12 h light/dark cycle, 22  ±
2  °C)  with  food  and  water  ad libitum. ·Experimental
females were fed with 50% of the total amount of food consumed by the controls, during 6 weeks. The ACTH cells were stained using the peroxidase-antiperoxidase immunohistochemical procedure and stereological analyses were conducted. The blood ACTH was measured by radioimmunoassay (ACTH-IMMULITE kit).   In   the   experimental   group   body   weight was (p<0.05) decreased by 48.8%, while the relative pituitary weight was (p<0.05) increased by 76 .9% in comparison   with   corresponding   parameters   ir1 controls . The volume of ACTH cells, as well as their volume density was significantly increased (p< 0.05 ) in the experimental group, by 17 .6% and 1 2.5% respectively. The blood ACTH level was increased (p<0.05) in experimental group by  13.4% .  Our findings show that the DR in peripubertal female rats stimulates the stress system, which was confirmed by increased morphofunctional parameters of ACTH ce lls .
.
PB  - Belgrade: Society of Medical Biochemists of Serbia
C3  - 17th Congress of Medical Biochemistry and Laboratory Medicine, with international participations, 6th EFCC Symposium for Balkan Region; 2010 Oct 5-9; Belgrade, Serbia.
T1  - Dietary restriction affectsS ACTH Cells of peripubertal female rats
SP  - 454
EP  - 455
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6100
ER  - 

@conference{
author = "Milošević, Verica and Ajdžanović, Vladimir and Trifunović, Svetlana and Bogojević, Desanka and Ivanović-Matić, Svetlana and Martinović, Vesna and Grigorov, Ilijana",
year = "2010",
abstract = "Dietary restriction (DR) in female rats pre­ sumably activates the stress (hypothalamo-pituitary­ adrenal) system. It's well known that various pituitary cell types are involved in the reaction to stress, i.e. adrenocorticotropic hormone (ACTH), prolactin and growth hormone cells. The aim of this study was to examine the effects of DR on the immunohistomor­ phometric features of pituitary ACTH cells and blood levels of ACTH. Peripubertal (38 days old) female Wistar rats were housed one per cage, under standard environmental conditions (12 h light/dark cycle, 22  ±
2  °C)  with  food  and  water  ad libitum. ·Experimental
females were fed with 50% of the total amount of food consumed by the controls, during 6 weeks. The ACTH cells were stained using the peroxidase-antiperoxidase immunohistochemical procedure and stereological analyses were conducted. The blood ACTH was measured by radioimmunoassay (ACTH-IMMULITE kit).   In   the   experimental   group   body   weight was (p<0.05) decreased by 48.8%, while the relative pituitary weight was (p<0.05) increased by 76 .9% in comparison   with   corresponding   parameters   ir1 controls . The volume of ACTH cells, as well as their volume density was significantly increased (p< 0.05 ) in the experimental group, by 17 .6% and 1 2.5% respectively. The blood ACTH level was increased (p<0.05) in experimental group by  13.4% .  Our findings show that the DR in peripubertal female rats stimulates the stress system, which was confirmed by increased morphofunctional parameters of ACTH ce lls .
.",
publisher = "Belgrade: Society of Medical Biochemists of Serbia",
journal = "17th Congress of Medical Biochemistry and Laboratory Medicine, with international participations, 6th EFCC Symposium for Balkan Region; 2010 Oct 5-9; Belgrade, Serbia.",
title = "Dietary restriction affectsS ACTH Cells of peripubertal female rats",
pages = "454-455",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6100"
}

Milošević, V., Ajdžanović, V., Trifunović, S., Bogojević, D., Ivanović-Matić, S., Martinović, V.,& Grigorov, I.. (2010). Dietary restriction affectsS ACTH Cells of peripubertal female rats. in 17th Congress of Medical Biochemistry and Laboratory Medicine, with international participations, 6th EFCC Symposium for Balkan Region; 2010 Oct 5-9; Belgrade, Serbia.
Belgrade: Society of Medical Biochemists of Serbia., 454-455.
https://hdl.handle.net/21.15107/rcub_ibiss_6100

Milošević V, Ajdžanović V, Trifunović S, Bogojević D, Ivanović-Matić S, Martinović V, Grigorov I. Dietary restriction affectsS ACTH Cells of peripubertal female rats. in 17th Congress of Medical Biochemistry and Laboratory Medicine, with international participations, 6th EFCC Symposium for Balkan Region; 2010 Oct 5-9; Belgrade, Serbia.. 2010;:454-455.
https://hdl.handle.net/21.15107/rcub_ibiss_6100 .

Milošević, Verica, Ajdžanović, Vladimir, Trifunović, Svetlana, Bogojević, Desanka, Ivanović-Matić, Svetlana, Martinović, Vesna, Grigorov, Ilijana, "Dietary restriction affectsS ACTH Cells of peripubertal female rats" in 17th Congress of Medical Biochemistry and Laboratory Medicine, with international participations, 6th EFCC Symposium for Balkan Region; 2010 Oct 5-9; Belgrade, Serbia. (2010):454-455,
https://hdl.handle.net/21.15107/rcub_ibiss_6100 .

TY  - JOUR
AU  - Mihailović, Mirjana
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Vidaković, Melita
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Ivanović Matić, Svetlana
AU  - Petrović, Miodrag
AU  - Labus-Blagojević, Svetlana
AU  - Martinović, Vesna
AU  - Arambašić Jovanović, Jelena
AU  - Poznanović, Goran
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1342
AB  - Background and Purpose CYP1A is one of the most sensitive biomarkers in fish of exposure to polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) These compounds induce a dose-dependent transcriptional induction of the CYP1A gene that results in increased protein concentrations The aim of this study was the detection of changes in CYP1A protein levels as a result of the exposure of fish to PAHs and PCBs at the mouth of the river Bojana in the spring Materials and Methods The PAH and PCB contents in seawater and sediment were determined by gas chromatography CYP1A induction was examined in the hepatopancreas of Red mullet (Mullus barbatus), European hake (Merluccius merluccius) and Tub gurnard (Trigla lucerna) by immunoblot analysis Results and Conclusions Chemical analyses of seawater and sediment revealed the presence of several PAHs and PCBs CYP1A was detected in the hepatic microsomal fraction in all examined fish species as an adaptive response to the presence of pollutants The reported changes at the molecular level represent an early-warning signal that reflected the biological response of fish to elevated toxin concentrations in the environment
PB  - Zagreb: Hrvatsko prirodoslovno društvo
T2  - Periodicum Biologorum
T1  - CYP1A expression in the hepatopancreas of Mullus barbatus, Merluccius merluccius and Trigla lucerna at the mouth of the river Bojana
IS  - 2
VL  - 112
SP  - 167
EP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1342
ER  - 

@article{
author = "Mihailović, Mirjana and Grdović, Nevena and Dinić, Svetlana and Uskoković, Aleksandra and Vidaković, Melita and Grigorov, Ilijana and Bogojević, Desanka and Ivanović Matić, Svetlana and Petrović, Miodrag and Labus-Blagojević, Svetlana and Martinović, Vesna and Arambašić Jovanović, Jelena and Poznanović, Goran",
year = "2010",
abstract = "Background and Purpose CYP1A is one of the most sensitive biomarkers in fish of exposure to polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) These compounds induce a dose-dependent transcriptional induction of the CYP1A gene that results in increased protein concentrations The aim of this study was the detection of changes in CYP1A protein levels as a result of the exposure of fish to PAHs and PCBs at the mouth of the river Bojana in the spring Materials and Methods The PAH and PCB contents in seawater and sediment were determined by gas chromatography CYP1A induction was examined in the hepatopancreas of Red mullet (Mullus barbatus), European hake (Merluccius merluccius) and Tub gurnard (Trigla lucerna) by immunoblot analysis Results and Conclusions Chemical analyses of seawater and sediment revealed the presence of several PAHs and PCBs CYP1A was detected in the hepatic microsomal fraction in all examined fish species as an adaptive response to the presence of pollutants The reported changes at the molecular level represent an early-warning signal that reflected the biological response of fish to elevated toxin concentrations in the environment",
publisher = "Zagreb: Hrvatsko prirodoslovno društvo",
journal = "Periodicum Biologorum",
title = "CYP1A expression in the hepatopancreas of Mullus barbatus, Merluccius merluccius and Trigla lucerna at the mouth of the river Bojana",
number = "2",
volume = "112",
pages = "167-171",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1342"
}

Mihailović, M., Grdović, N., Dinić, S., Uskoković, A., Vidaković, M., Grigorov, I., Bogojević, D., Ivanović Matić, S., Petrović, M., Labus-Blagojević, S., Martinović, V., Arambašić Jovanović, J.,& Poznanović, G.. (2010). CYP1A expression in the hepatopancreas of Mullus barbatus, Merluccius merluccius and Trigla lucerna at the mouth of the river Bojana. in Periodicum Biologorum
Zagreb: Hrvatsko prirodoslovno društvo., 112(2), 167-171.
https://hdl.handle.net/21.15107/rcub_ibiss_1342

Mihailović M, Grdović N, Dinić S, Uskoković A, Vidaković M, Grigorov I, Bogojević D, Ivanović Matić S, Petrović M, Labus-Blagojević S, Martinović V, Arambašić Jovanović J, Poznanović G. CYP1A expression in the hepatopancreas of Mullus barbatus, Merluccius merluccius and Trigla lucerna at the mouth of the river Bojana. in Periodicum Biologorum. 2010;112(2):167-171.
https://hdl.handle.net/21.15107/rcub_ibiss_1342 .

Mihailović, Mirjana, Grdović, Nevena, Dinić, Svetlana, Uskoković, Aleksandra, Vidaković, Melita, Grigorov, Ilijana, Bogojević, Desanka, Ivanović Matić, Svetlana, Petrović, Miodrag, Labus-Blagojević, Svetlana, Martinović, Vesna, Arambašić Jovanović, Jelena, Poznanović, Goran, "CYP1A expression in the hepatopancreas of Mullus barbatus, Merluccius merluccius and Trigla lucerna at the mouth of the river Bojana" in Periodicum Biologorum, 112, no. 2 (2010):167-171,
https://hdl.handle.net/21.15107/rcub_ibiss_1342 .

TY  - JOUR
AU  - Mihailović, Mirjana
AU  - Poznanović, Goran
AU  - Grdović, Nevena
AU  - Vidaković, Melita
AU  - Dinić, Svetlana
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1349
AB  - Previously we reported that elevated circulating concentrations of the acute-phase (AP) protein alpha(2)-macroglobulin (alpha M-2), either as typically occurring in pregnant female rats or after administration to male rats, provides radioprotection, displayed as 100% survival of experimental animals exposed to total-body irradiation with 6.7 Gy (LD50/30) x-rays, that is as effective as that afforded by the synthetic radioprotector amifostine. The finding that amifostine administration induces a 45-fold increase in alpha M-2 in the circulation led us to hypothesise that alpha M-2 assumes an essential role in both natural and amifostine-mediated radioprotection in the rat. In the present work we examined the activation of cytoprotective mechanisms in rat hepatocytes after the exogenous administration of alpha M-2 and amifostine. Our results showed that the IL6/JAK/STAT3 hepatoprotective signal pathway, described in a variety of liver-injury models, upregulated the alpha M-2 gene in amifostine-pretreated animals. In both alpha M-2- and amifostine-pretreated rats we observed the activation of the Akt signalling pathways that mediate cellular survival. At the cellular level this was reflected as a significant reduction of irradiation-induced DNA damage that allowed for the rapid and complete restoration of liver mass and ultimately at the level of the whole organism the complete restoration of body weight. We conclude that the selective upregulation of alpha M-2 plays a central role in amifostine-provided radioprotection.
PB  - IOP Publishing Ltd
T2  - Journal of Radiological Protection
T1  - The rat acute-phase protein alpha(2)-macroglobulin plays a central role in amifostine-mediated radioprotection
IS  - 3
VL  - 30
DO  - 10.1088/0952-4746/30/3/011
SP  - 567
EP  - 583
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1349
ER  - 

@article{
author = "Mihailović, Mirjana and Poznanović, Goran and Grdović, Nevena and Vidaković, Melita and Dinić, Svetlana and Grigorov, Ilijana and Bogojević, Desanka",
year = "2010",
abstract = "Previously we reported that elevated circulating concentrations of the acute-phase (AP) protein alpha(2)-macroglobulin (alpha M-2), either as typically occurring in pregnant female rats or after administration to male rats, provides radioprotection, displayed as 100% survival of experimental animals exposed to total-body irradiation with 6.7 Gy (LD50/30) x-rays, that is as effective as that afforded by the synthetic radioprotector amifostine. The finding that amifostine administration induces a 45-fold increase in alpha M-2 in the circulation led us to hypothesise that alpha M-2 assumes an essential role in both natural and amifostine-mediated radioprotection in the rat. In the present work we examined the activation of cytoprotective mechanisms in rat hepatocytes after the exogenous administration of alpha M-2 and amifostine. Our results showed that the IL6/JAK/STAT3 hepatoprotective signal pathway, described in a variety of liver-injury models, upregulated the alpha M-2 gene in amifostine-pretreated animals. In both alpha M-2- and amifostine-pretreated rats we observed the activation of the Akt signalling pathways that mediate cellular survival. At the cellular level this was reflected as a significant reduction of irradiation-induced DNA damage that allowed for the rapid and complete restoration of liver mass and ultimately at the level of the whole organism the complete restoration of body weight. We conclude that the selective upregulation of alpha M-2 plays a central role in amifostine-provided radioprotection.",
publisher = "IOP Publishing Ltd",
journal = "Journal of Radiological Protection",
title = "The rat acute-phase protein alpha(2)-macroglobulin plays a central role in amifostine-mediated radioprotection",
number = "3",
volume = "30",
doi = "10.1088/0952-4746/30/3/011",
pages = "567-583",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1349"
}

Mihailović, M., Poznanović, G., Grdović, N., Vidaković, M., Dinić, S., Grigorov, I.,& Bogojević, D.. (2010). The rat acute-phase protein alpha(2)-macroglobulin plays a central role in amifostine-mediated radioprotection. in Journal of Radiological Protection
IOP Publishing Ltd., 30(3), 567-583.
https://doi.org/10.1088/0952-4746/30/3/011
https://hdl.handle.net/21.15107/rcub_ibiss_1349

Mihailović M, Poznanović G, Grdović N, Vidaković M, Dinić S, Grigorov I, Bogojević D. The rat acute-phase protein alpha(2)-macroglobulin plays a central role in amifostine-mediated radioprotection. in Journal of Radiological Protection. 2010;30(3):567-583.
doi:10.1088/0952-4746/30/3/011
https://hdl.handle.net/21.15107/rcub_ibiss_1349 .

Mihailović, Mirjana, Poznanović, Goran, Grdović, Nevena, Vidaković, Melita, Dinić, Svetlana, Grigorov, Ilijana, Bogojević, Desanka, "The rat acute-phase protein alpha(2)-macroglobulin plays a central role in amifostine-mediated radioprotection" in Journal of Radiological Protection, 30, no. 3 (2010):567-583,
https://doi.org/10.1088/0952-4746/30/3/011 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1349 .

TY  - JOUR
AU  - Grdović, Nevena
AU  - Vidaković, Melita
AU  - Mihailović, Mirjana
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Poznanović, Goran
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1369
AB  - Although cryosurgery is attaining increasing clinical acceptance, our understanding of the mechanisms of cryogenic cell destruction remains incomplete. While it is generally accepted that cryoinjured cells die by necrosis, the involvement of apoptosis was recently shown. Our studies of liver cell death by cryogenic temperature revealed the activation of endonuclease p23 and its de novo association with the nuclear matrix. This finding is strongly suggestive of a programmed-type of cell death process. The presumed order underlying cryonecrotic cell death is addressed here by examining the mechanism of p23 activation. To that end, nuclear proteins that were prepared from fresh liver, which is devoid of p23 activity, were incubated with protein fractions isolated from liver exposed to freezing/thawing that possessed a presumed p23 activation factor. We observed that the activation of p23 was the result of a proteolytic event in which cathepsin D played a major role. Different patterns of proteolytic cleavage of nuclear proteins after in vitro incubation of nuclei and in samples isolated from frozen/thawed liver were observed. Although both processes induced p23 activation, the incubation experiments generated proteolytic hallmarks of apoptosis, while freezing/thawing of whole liver resulted in typical necrotic PARP-1 cleavage products and intact lamin B. As an explanation we offer a hypothesis that after freezing, cells possess the potential to die through necrotic as well as apoptotic mechanisms, based on our finding that the cytosol of cells exposed to cryogenic temperatures contains both necrotic and apoptotic executors of cell death. (C) 2010 Elsevier Inc. All rights reserved.
PB  - Elsevier
T2  - Cryobiology
T1  - Proteolytic events in cryonecrotic cell death: Proteolytic activation of endonuclease P23
IS  - 3
VL  - 60
DO  - 10.1016/j.cryobiol.2010.01.005
SP  - 271
EP  - 280
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1369
ER  - 

@article{
author = "Grdović, Nevena and Vidaković, Melita and Mihailović, Mirjana and Dinić, Svetlana and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Poznanović, Goran",
year = "2010",
abstract = "Although cryosurgery is attaining increasing clinical acceptance, our understanding of the mechanisms of cryogenic cell destruction remains incomplete. While it is generally accepted that cryoinjured cells die by necrosis, the involvement of apoptosis was recently shown. Our studies of liver cell death by cryogenic temperature revealed the activation of endonuclease p23 and its de novo association with the nuclear matrix. This finding is strongly suggestive of a programmed-type of cell death process. The presumed order underlying cryonecrotic cell death is addressed here by examining the mechanism of p23 activation. To that end, nuclear proteins that were prepared from fresh liver, which is devoid of p23 activity, were incubated with protein fractions isolated from liver exposed to freezing/thawing that possessed a presumed p23 activation factor. We observed that the activation of p23 was the result of a proteolytic event in which cathepsin D played a major role. Different patterns of proteolytic cleavage of nuclear proteins after in vitro incubation of nuclei and in samples isolated from frozen/thawed liver were observed. Although both processes induced p23 activation, the incubation experiments generated proteolytic hallmarks of apoptosis, while freezing/thawing of whole liver resulted in typical necrotic PARP-1 cleavage products and intact lamin B. As an explanation we offer a hypothesis that after freezing, cells possess the potential to die through necrotic as well as apoptotic mechanisms, based on our finding that the cytosol of cells exposed to cryogenic temperatures contains both necrotic and apoptotic executors of cell death. (C) 2010 Elsevier Inc. All rights reserved.",
publisher = "Elsevier",
journal = "Cryobiology",
title = "Proteolytic events in cryonecrotic cell death: Proteolytic activation of endonuclease P23",
number = "3",
volume = "60",
doi = "10.1016/j.cryobiol.2010.01.005",
pages = "271-280",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1369"
}

Grdović, N., Vidaković, M., Mihailović, M., Dinić, S., Uskoković, A., Arambašić Jovanović, J.,& Poznanović, G.. (2010). Proteolytic events in cryonecrotic cell death: Proteolytic activation of endonuclease P23. in Cryobiology
Elsevier., 60(3), 271-280.
https://doi.org/10.1016/j.cryobiol.2010.01.005
https://hdl.handle.net/21.15107/rcub_ibiss_1369

Grdović N, Vidaković M, Mihailović M, Dinić S, Uskoković A, Arambašić Jovanović J, Poznanović G. Proteolytic events in cryonecrotic cell death: Proteolytic activation of endonuclease P23. in Cryobiology. 2010;60(3):271-280.
doi:10.1016/j.cryobiol.2010.01.005
https://hdl.handle.net/21.15107/rcub_ibiss_1369 .

Grdović, Nevena, Vidaković, Melita, Mihailović, Mirjana, Dinić, Svetlana, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Poznanović, Goran, "Proteolytic events in cryonecrotic cell death: Proteolytic activation of endonuclease P23" in Cryobiology, 60, no. 3 (2010):271-280,
https://doi.org/10.1016/j.cryobiol.2010.01.005 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1369 .

TY  - JOUR
AU  - Mihailović, Mirjana
AU  - Petrović, Miodrag
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Vidaković, Melita
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Ivanović Matić, Svetlana
AU  - Martinović, Vesna
AU  - Arambašić Jovanović, Jelena
AU  - Joksimović, Danijela
AU  - Labus-Blagojević, Svetlana
AU  - Poznanović, Goran
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/561
AB  - The enzyme CYP1A is an established biomarker of fish exposure to polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs). The metallothioneins (MT), a family of Cys-rich proteins, bind a wide range of metals and participate in their metabolism. The aim of the study was to examine the correlation between CYP1A and MT expression in commercially important fish species Mullus barbatus and Merluccius merluccius and contaminants (PAHs, PCBs, toxic metals) in seawater and sediment from three localities with different level of contamination in the Adriatic Sea in winter, i.e., Platamuni, Valdanos and the port of Bar. The relative concentration of CYP1A was the highest in both fish species from Bar. Increased concentrations of PCBs in the seawater were observed only in Bar. A species-specific higher increase in the protein concentration of CYP1A was observed in Mullus barbatus compared to Merluccius merluccius. The levels of MT were the highest in Merluccius merluccius from Bar and in Mullus barbatus from Valdanos. The induction of MT correlated with the elevated concentrations of Cu and Pb determined by chemical analysis of the seawater from Bar and Valdanos, respectively. According to the chemical analysis of the seawater and the biological response of the fish, the Platamuni locality exhibited the lowest level of contamination.
AB  - CYP1A predstavlja dobro okarakterisan biomarker kod riba pri izlaganju policikličnim aromatičnim ugljovodonicima (PAH) i policikličnim bifeholima (PCB). Metalotioneini (MT) pretstavljaju familiju proteina koji vezuju metale i učestvuju u njihovoj metabolizmu, transportu i regulaciji. Cilj ovog rada je bio da se ispitaju korelacije između promena u nivou CYP1A i MT u hepatopankreasu dve komercijalno važne ribe: Mullus barbatus (trlja) i Merluccius merluccius (oslić) i kontaminanatata: PAH, PCB i toksičnih metala) na tri lokaliteta u Jadrnskom moru (Platamuni, Valdanos i luka Bar) u zimu. CYP1A je u najvećem stepenu indukovan na lokalitetu Bar u obe ispitivane vrste, što je u korelaciji sa prisustvom povećane količine PCB u morskoj vodi u Baru. Nivo MT je najveći kod oslića izlovljenog u Baru, a kod trlje u Valdanosu. To je u korelaciji sa izmerenim povećanim koncentracijama bakra u Baru, a olova u Valdanosu. Na osnovu izučavanih parametara, Platamuni su lokalitet sa najmanjim stepenom kontaminacije.
PB  - Belgrade: Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Ekspresija CYPLA i metalotioneina u hepatopankreasu oslića i trlje iz Jadranskog mora
T1  - CYP1A and metallothionein expression in the hepatopancreas of merluccius merluccius and mullus barbatus from the Adriatic sea
IS  - 8
VL  - 75
DO  - 10.2298/JSC091029083M
SP  - 1149
EP  - 1159
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_561
ER  - 

@article{
author = "Mihailović, Mirjana and Petrović, Miodrag and Grdović, Nevena and Dinić, Svetlana and Uskoković, Aleksandra and Vidaković, Melita and Grigorov, Ilijana and Bogojević, Desanka and Ivanović Matić, Svetlana and Martinović, Vesna and Arambašić Jovanović, Jelena and Joksimović, Danijela and Labus-Blagojević, Svetlana and Poznanović, Goran",
year = "2010, 2010",
abstract = "The enzyme CYP1A is an established biomarker of fish exposure to polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs). The metallothioneins (MT), a family of Cys-rich proteins, bind a wide range of metals and participate in their metabolism. The aim of the study was to examine the correlation between CYP1A and MT expression in commercially important fish species Mullus barbatus and Merluccius merluccius and contaminants (PAHs, PCBs, toxic metals) in seawater and sediment from three localities with different level of contamination in the Adriatic Sea in winter, i.e., Platamuni, Valdanos and the port of Bar. The relative concentration of CYP1A was the highest in both fish species from Bar. Increased concentrations of PCBs in the seawater were observed only in Bar. A species-specific higher increase in the protein concentration of CYP1A was observed in Mullus barbatus compared to Merluccius merluccius. The levels of MT were the highest in Merluccius merluccius from Bar and in Mullus barbatus from Valdanos. The induction of MT correlated with the elevated concentrations of Cu and Pb determined by chemical analysis of the seawater from Bar and Valdanos, respectively. According to the chemical analysis of the seawater and the biological response of the fish, the Platamuni locality exhibited the lowest level of contamination., CYP1A predstavlja dobro okarakterisan biomarker kod riba pri izlaganju policikličnim aromatičnim ugljovodonicima (PAH) i policikličnim bifeholima (PCB). Metalotioneini (MT) pretstavljaju familiju proteina koji vezuju metale i učestvuju u njihovoj metabolizmu, transportu i regulaciji. Cilj ovog rada je bio da se ispitaju korelacije između promena u nivou CYP1A i MT u hepatopankreasu dve komercijalno važne ribe: Mullus barbatus (trlja) i Merluccius merluccius (oslić) i kontaminanatata: PAH, PCB i toksičnih metala) na tri lokaliteta u Jadrnskom moru (Platamuni, Valdanos i luka Bar) u zimu. CYP1A je u najvećem stepenu indukovan na lokalitetu Bar u obe ispitivane vrste, što je u korelaciji sa prisustvom povećane količine PCB u morskoj vodi u Baru. Nivo MT je najveći kod oslića izlovljenog u Baru, a kod trlje u Valdanosu. To je u korelaciji sa izmerenim povećanim koncentracijama bakra u Baru, a olova u Valdanosu. Na osnovu izučavanih parametara, Platamuni su lokalitet sa najmanjim stepenom kontaminacije.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Ekspresija CYPLA i metalotioneina u hepatopankreasu oslića i trlje iz Jadranskog mora, CYP1A and metallothionein expression in the hepatopancreas of merluccius merluccius and mullus barbatus from the Adriatic sea",
number = "8",
volume = "75",
doi = "10.2298/JSC091029083M",
pages = "1149-1159",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_561"
}

Mihailović, M., Petrović, M., Grdović, N., Dinić, S., Uskoković, A., Vidaković, M., Grigorov, I., Bogojević, D., Ivanović Matić, S., Martinović, V., Arambašić Jovanović, J., Joksimović, D., Labus-Blagojević, S.,& Poznanović, G.. (2010). Ekspresija CYPLA i metalotioneina u hepatopankreasu oslića i trlje iz Jadranskog mora. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Society., 75(8), 1149-1159.
https://doi.org/10.2298/JSC091029083M
https://hdl.handle.net/21.15107/rcub_ibiss_561

Mihailović M, Petrović M, Grdović N, Dinić S, Uskoković A, Vidaković M, Grigorov I, Bogojević D, Ivanović Matić S, Martinović V, Arambašić Jovanović J, Joksimović D, Labus-Blagojević S, Poznanović G. Ekspresija CYPLA i metalotioneina u hepatopankreasu oslića i trlje iz Jadranskog mora. in Journal of the Serbian Chemical Society. 2010;75(8):1149-1159.
doi:10.2298/JSC091029083M
https://hdl.handle.net/21.15107/rcub_ibiss_561 .

Mihailović, Mirjana, Petrović, Miodrag, Grdović, Nevena, Dinić, Svetlana, Uskoković, Aleksandra, Vidaković, Melita, Grigorov, Ilijana, Bogojević, Desanka, Ivanović Matić, Svetlana, Martinović, Vesna, Arambašić Jovanović, Jelena, Joksimović, Danijela, Labus-Blagojević, Svetlana, Poznanović, Goran, "Ekspresija CYPLA i metalotioneina u hepatopankreasu oslića i trlje iz Jadranskog mora" in Journal of the Serbian Chemical Society, 75, no. 8 (2010):1149-1159,
https://doi.org/10.2298/JSC091029083M .,
https://hdl.handle.net/21.15107/rcub_ibiss_561 .

TY  - JOUR
AU  - Ivanović-Matić, Svetlana
AU  - Mihailović, Mirjana
AU  - Dinić, Svetlana
AU  - Martinović, Vesna
AU  - Bogojević, Desanka
AU  - Grigorov, Ilijana
AU  - Poznanović, Goran
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5542
AB  - The activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST), the incidence of DNA damage, the activation of poly (ADP-ribose) polymerase-1 (PARP-1), a marker of DNA repair, and connective tissue growth factor (CTGF), a marker of tissue fibrosis, were examined in the hearts of rats for 16 weeks after diabetes induction by streptozotocin (STZ) administration. A 150% increase in CAT activity was detected at the end of the 2nd week post-STZ administration, and CAT activity remained 80% above the control level throughout 16 weeks. While total SOD and CuZn-SOD exhibited progressively decreasing activities, those of Mn-SOD and GST were elevated. Neither DNA strand breaks (apoptosis or necrosis) nor changes in PARP-1 activity and in CTGF levels (fibrosis) were observed in the diabetic heart. The absence of cardiomyopathy is accompanied with increased activities of CAT, MnSOD and GST.
PB  - London: BMC
T2  - The Journal of Physiological Sciences
T1  - The absence of cardiomyopathy is accompanied by increased activities of CAT, MnSOD and GST in long-term diabetes n rats.
IS  - 4
VL  - 60
DO  - 10.1007/s12576-010-0093-z
SP  - 259
EP  - 266
ER  - 

@article{
author = "Ivanović-Matić, Svetlana and Mihailović, Mirjana and Dinić, Svetlana and Martinović, Vesna and Bogojević, Desanka and Grigorov, Ilijana and Poznanović, Goran",
year = "2010",
abstract = "The activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST), the incidence of DNA damage, the activation of poly (ADP-ribose) polymerase-1 (PARP-1), a marker of DNA repair, and connective tissue growth factor (CTGF), a marker of tissue fibrosis, were examined in the hearts of rats for 16 weeks after diabetes induction by streptozotocin (STZ) administration. A 150% increase in CAT activity was detected at the end of the 2nd week post-STZ administration, and CAT activity remained 80% above the control level throughout 16 weeks. While total SOD and CuZn-SOD exhibited progressively decreasing activities, those of Mn-SOD and GST were elevated. Neither DNA strand breaks (apoptosis or necrosis) nor changes in PARP-1 activity and in CTGF levels (fibrosis) were observed in the diabetic heart. The absence of cardiomyopathy is accompanied with increased activities of CAT, MnSOD and GST.",
publisher = "London: BMC",
journal = "The Journal of Physiological Sciences",
title = "The absence of cardiomyopathy is accompanied by increased activities of CAT, MnSOD and GST in long-term diabetes n rats.",
number = "4",
volume = "60",
doi = "10.1007/s12576-010-0093-z",
pages = "259-266"
}

Ivanović-Matić, S., Mihailović, M., Dinić, S., Martinović, V., Bogojević, D., Grigorov, I.,& Poznanović, G.. (2010). The absence of cardiomyopathy is accompanied by increased activities of CAT, MnSOD and GST in long-term diabetes n rats.. in The Journal of Physiological Sciences
London: BMC., 60(4), 259-266.
https://doi.org/10.1007/s12576-010-0093-z

Ivanović-Matić S, Mihailović M, Dinić S, Martinović V, Bogojević D, Grigorov I, Poznanović G. The absence of cardiomyopathy is accompanied by increased activities of CAT, MnSOD and GST in long-term diabetes n rats.. in The Journal of Physiological Sciences. 2010;60(4):259-266.
doi:10.1007/s12576-010-0093-z .

Ivanović-Matić, Svetlana, Mihailović, Mirjana, Dinić, Svetlana, Martinović, Vesna, Bogojević, Desanka, Grigorov, Ilijana, Poznanović, Goran, "The absence of cardiomyopathy is accompanied by increased activities of CAT, MnSOD and GST in long-term diabetes n rats." in The Journal of Physiological Sciences, 60, no. 4 (2010):259-266,
https://doi.org/10.1007/s12576-010-0093-z . .

TY  - JOUR
AU  - Mihailović, Mirjana
AU  - Dobrić, Silva Lj
AU  - Poznanović, Goran
AU  - Petrović, Miodrag V
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Bogojević, Desanka
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1449
AB  - The importance of alpha(2)-macroglobulin (alpha(2)M) in natural radioprotection was studied by examining its radioprotective effectiveness in rat models of exogenously and endogenously, preexposure-increased alpha(2)M. Radioprotective efficacy was ascertained by the postirradiation survival rate, the restoration of body weight, and the leukocyte count, which were monitored during a 4-week follow-up period. The results were compared with the effects of a pretreatment with the synthetic radioprotective agent amifostine (Ami), which provides 100% protection in rats whole-body-irradiated by x-rays given in a dose of 6.7 Gy (LD(50/30)). Raising the plasma concentration of alpha(2)M 15-fold in male rats by a single intraperitoneal injection of purified protein provided 100% survival of irradiated animals. Female rats on the 19th day of pregnancy with endogenously elevated levels of alpha(2)M displayed improved survival (80%) compared with untreated rats (50% survival). After alpha(2)M administration, the pregnant, irradiated rats exhibited 100% survival. In both males and pregnant females, alpha(2)M administration promoted body weight and leukocyte postirradiation recovery as in Ami-pretreated rats. These findings, together with our observation that Ami administration induced a 45-fold increase in alpha(2)M in the circulation, led us to conclude that alpha(2)M has an essential role in both natural and amifostine-mediated radioprotection in the rat.
PB  - Wolters Kluwer Health, Inc.
T2  - Shock
T1  - The Acute-Phase Protein Alpha(2)-Macroglobulin Plays An Important Role in Radioprotection in the Rat
IS  - 6
VL  - 31
DO  - 10.1097/SHK.0b013e31818bb625
SP  - 607
EP  - 614
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1449
ER  - 

@article{
author = "Mihailović, Mirjana and Dobrić, Silva Lj and Poznanović, Goran and Petrović, Miodrag V and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Bogojević, Desanka",
year = "2009",
abstract = "The importance of alpha(2)-macroglobulin (alpha(2)M) in natural radioprotection was studied by examining its radioprotective effectiveness in rat models of exogenously and endogenously, preexposure-increased alpha(2)M. Radioprotective efficacy was ascertained by the postirradiation survival rate, the restoration of body weight, and the leukocyte count, which were monitored during a 4-week follow-up period. The results were compared with the effects of a pretreatment with the synthetic radioprotective agent amifostine (Ami), which provides 100% protection in rats whole-body-irradiated by x-rays given in a dose of 6.7 Gy (LD(50/30)). Raising the plasma concentration of alpha(2)M 15-fold in male rats by a single intraperitoneal injection of purified protein provided 100% survival of irradiated animals. Female rats on the 19th day of pregnancy with endogenously elevated levels of alpha(2)M displayed improved survival (80%) compared with untreated rats (50% survival). After alpha(2)M administration, the pregnant, irradiated rats exhibited 100% survival. In both males and pregnant females, alpha(2)M administration promoted body weight and leukocyte postirradiation recovery as in Ami-pretreated rats. These findings, together with our observation that Ami administration induced a 45-fold increase in alpha(2)M in the circulation, led us to conclude that alpha(2)M has an essential role in both natural and amifostine-mediated radioprotection in the rat.",
publisher = "Wolters Kluwer Health, Inc.",
journal = "Shock",
title = "The Acute-Phase Protein Alpha(2)-Macroglobulin Plays An Important Role in Radioprotection in the Rat",
number = "6",
volume = "31",
doi = "10.1097/SHK.0b013e31818bb625",
pages = "607-614",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1449"
}

Mihailović, M., Dobrić, S. L., Poznanović, G., Petrović, M. V., Uskoković, A., Arambašić Jovanović, J.,& Bogojević, D.. (2009). The Acute-Phase Protein Alpha(2)-Macroglobulin Plays An Important Role in Radioprotection in the Rat. in Shock
Wolters Kluwer Health, Inc.., 31(6), 607-614.
https://doi.org/10.1097/SHK.0b013e31818bb625
https://hdl.handle.net/21.15107/rcub_ibiss_1449

Mihailović M, Dobrić SL, Poznanović G, Petrović MV, Uskoković A, Arambašić Jovanović J, Bogojević D. The Acute-Phase Protein Alpha(2)-Macroglobulin Plays An Important Role in Radioprotection in the Rat. in Shock. 2009;31(6):607-614.
doi:10.1097/SHK.0b013e31818bb625
https://hdl.handle.net/21.15107/rcub_ibiss_1449 .

Mihailović, Mirjana, Dobrić, Silva Lj, Poznanović, Goran, Petrović, Miodrag V, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Bogojević, Desanka, "The Acute-Phase Protein Alpha(2)-Macroglobulin Plays An Important Role in Radioprotection in the Rat" in Shock, 31, no. 6 (2009):607-614,
https://doi.org/10.1097/SHK.0b013e31818bb625 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1449 .

TY  - JOUR
AU  - Dinić, Svetlana
AU  - Mihailović, Mirjana
AU  - Ivanović Matić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Grdović, Nevena
AU  - Vidaković, Melita
AU  - Poznanović, Goran
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/197
AB  - Participation of the nuclear matrix in regulation of alpha-2-macroglobulin (α2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with the α2M gene promoter (-852/+12) and immunoblot analysis revealed diverse patterns of association of transcription factors with the nuclear matrix. HNF-6, C/EBPα, and STAT5b were involved in basal and C/EBPβ, STAT1, and STAT3 in AP-stimulated α2M expression. These findings support the assumption that transcription factor-nuclear matrix interactions serve to channel gene regulatory proteins to DNA sequences.
AB  - Cilj rada je ispitivanje učešća jedarnog matriksa u regulaciji transkripcije gena za alfa-2-makroglobulin tokom razvića jetre pacova i akutno faznog odgovora (AFO). Nakon DNK afinitetne hromatografije proteina unutrašnje mreže jedarnog matriksa fetalne i adultne jetre, u bazalnim i AFO uslovima, sa promotorskim elementom gena za α2M (-852/+12) i imunoblot analize, identifikovane su dinamičke asocijacije transkripcionih faktora uključenih u regulaciju ekspresije gena za α2M sa jedarnim matriksom. HNF-6, C/EBPα, STAT5b su uključeni u regulaciju bazalne ekspresije gena za α2M, dok C/EBPβ, STAT1, STAT3 posreduju u regulaciji ekspresije ovog gena tokom AFO. Opisane interakcije doprinose razumevanju predloženih mehanizama kojima se transkripcioni faktori usmeravaju ka ciljnim regulatornim elementima DNK.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Dinamičke asocijacije transkripcionih faktora sa nuklearnim matriksom jetre pacova su funkcionalno povezane sa diferencijalnom genskom ekspresijom alfa-2-makroglobulina
T1  - Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression
IS  - 3
VL  - 60
DO  - 10.2298/ABS0803355D
SP  - 355
EP  - 366
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_197
ER  - 

@article{
author = "Dinić, Svetlana and Mihailović, Mirjana and Ivanović Matić, Svetlana and Uskoković, Aleksandra and Grdović, Nevena and Vidaković, Melita and Poznanović, Goran",
year = "2008, 2008",
abstract = "Participation of the nuclear matrix in regulation of alpha-2-macroglobulin (α2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with the α2M gene promoter (-852/+12) and immunoblot analysis revealed diverse patterns of association of transcription factors with the nuclear matrix. HNF-6, C/EBPα, and STAT5b were involved in basal and C/EBPβ, STAT1, and STAT3 in AP-stimulated α2M expression. These findings support the assumption that transcription factor-nuclear matrix interactions serve to channel gene regulatory proteins to DNA sequences., Cilj rada je ispitivanje učešća jedarnog matriksa u regulaciji transkripcije gena za alfa-2-makroglobulin tokom razvića jetre pacova i akutno faznog odgovora (AFO). Nakon DNK afinitetne hromatografije proteina unutrašnje mreže jedarnog matriksa fetalne i adultne jetre, u bazalnim i AFO uslovima, sa promotorskim elementom gena za α2M (-852/+12) i imunoblot analize, identifikovane su dinamičke asocijacije transkripcionih faktora uključenih u regulaciju ekspresije gena za α2M sa jedarnim matriksom. HNF-6, C/EBPα, STAT5b su uključeni u regulaciju bazalne ekspresije gena za α2M, dok C/EBPβ, STAT1, STAT3 posreduju u regulaciji ekspresije ovog gena tokom AFO. Opisane interakcije doprinose razumevanju predloženih mehanizama kojima se transkripcioni faktori usmeravaju ka ciljnim regulatornim elementima DNK.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Dinamičke asocijacije transkripcionih faktora sa nuklearnim matriksom jetre pacova su funkcionalno povezane sa diferencijalnom genskom ekspresijom alfa-2-makroglobulina, Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression",
number = "3",
volume = "60",
doi = "10.2298/ABS0803355D",
pages = "355-366",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_197"
}

Dinić, S., Mihailović, M., Ivanović Matić, S., Uskoković, A., Grdović, N., Vidaković, M.,& Poznanović, G.. (2008). Dinamičke asocijacije transkripcionih faktora sa nuklearnim matriksom jetre pacova su funkcionalno povezane sa diferencijalnom genskom ekspresijom alfa-2-makroglobulina. in Archives of Biological Sciences
Serbian Biological Society., 60(3), 355-366.
https://doi.org/10.2298/ABS0803355D
https://hdl.handle.net/21.15107/rcub_ibiss_197

Dinić S, Mihailović M, Ivanović Matić S, Uskoković A, Grdović N, Vidaković M, Poznanović G. Dinamičke asocijacije transkripcionih faktora sa nuklearnim matriksom jetre pacova su funkcionalno povezane sa diferencijalnom genskom ekspresijom alfa-2-makroglobulina. in Archives of Biological Sciences. 2008;60(3):355-366.
doi:10.2298/ABS0803355D
https://hdl.handle.net/21.15107/rcub_ibiss_197 .

Dinić, Svetlana, Mihailović, Mirjana, Ivanović Matić, Svetlana, Uskoković, Aleksandra, Grdović, Nevena, Vidaković, Melita, Poznanović, Goran, "Dinamičke asocijacije transkripcionih faktora sa nuklearnim matriksom jetre pacova su funkcionalno povezane sa diferencijalnom genskom ekspresijom alfa-2-makroglobulina" in Archives of Biological Sciences, 60, no. 3 (2008):355-366,
https://doi.org/10.2298/ABS0803355D .,
https://hdl.handle.net/21.15107/rcub_ibiss_197 .

TY  - JOUR
AU  - Ivanović-Matić, Svetlana
AU  - Poznanović, Goran
AU  - Grigorov, Ilijana
AU  - Dinić, Svetlana
AU  - Mihailović, Mirjana
AU  - Grdović, Nevena
AU  - Uskoković, Aleksandra
AU  - Martinović, Vesna
AU  - Arambašić Jovanović, Jelena
AU  - Petrović, Miodrag
AU  - Bogojević, Desanka
PY  - 2008
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5543
AB  - The organophosphorus compounds soman and paraoxon induce the acute-phase (AP) response. All phases of the AP response, from macrophage activation and stimulation of glucocorticoid secretion to AP protein expression appear to be under the control of similar molecular mechanisms to those during the turpentine-induced AP response. The AP protein content in the circulation 24 h after either soman, paraoxon or turpentine administration was injury-specific. Both soman and paraoxon poisoning were characterized by significantly increased synthesis of alpha(1)-acid glycoprotein (AGP) that displayed an immunomodulatory effect in vitro. This result suggests that after organophosphate poisoning AGP participates in vivo in a negative feedback mechanism that prevents over-activity of the immune system.
PB  - Hoboken : John Wiley & Sons Ltd
T2  - Journal of Applied Toxicology
T1  - The organophosphate-induced acute-phase response is characterized by synthesis of α1-acid glycoprotein that exhibits an immunomodulatory effect
IS  - 1
VL  - 28
DO  - 10.1002/jat.1254
SP  - 63
EP  - 71
ER  - 

@article{
author = "Ivanović-Matić, Svetlana and Poznanović, Goran and Grigorov, Ilijana and Dinić, Svetlana and Mihailović, Mirjana and Grdović, Nevena and Uskoković, Aleksandra and Martinović, Vesna and Arambašić Jovanović, Jelena and Petrović, Miodrag and Bogojević, Desanka",
year = "2008",
abstract = "The organophosphorus compounds soman and paraoxon induce the acute-phase (AP) response. All phases of the AP response, from macrophage activation and stimulation of glucocorticoid secretion to AP protein expression appear to be under the control of similar molecular mechanisms to those during the turpentine-induced AP response. The AP protein content in the circulation 24 h after either soman, paraoxon or turpentine administration was injury-specific. Both soman and paraoxon poisoning were characterized by significantly increased synthesis of alpha(1)-acid glycoprotein (AGP) that displayed an immunomodulatory effect in vitro. This result suggests that after organophosphate poisoning AGP participates in vivo in a negative feedback mechanism that prevents over-activity of the immune system.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "Journal of Applied Toxicology",
title = "The organophosphate-induced acute-phase response is characterized by synthesis of α1-acid glycoprotein that exhibits an immunomodulatory effect",
number = "1",
volume = "28",
doi = "10.1002/jat.1254",
pages = "63-71"
}

Ivanović-Matić, S., Poznanović, G., Grigorov, I., Dinić, S., Mihailović, M., Grdović, N., Uskoković, A., Martinović, V., Arambašić Jovanović, J., Petrović, M.,& Bogojević, D.. (2008). The organophosphate-induced acute-phase response is characterized by synthesis of α1-acid glycoprotein that exhibits an immunomodulatory effect. in Journal of Applied Toxicology
Hoboken : John Wiley & Sons Ltd., 28(1), 63-71.
https://doi.org/10.1002/jat.1254

Ivanović-Matić S, Poznanović G, Grigorov I, Dinić S, Mihailović M, Grdović N, Uskoković A, Martinović V, Arambašić Jovanović J, Petrović M, Bogojević D. The organophosphate-induced acute-phase response is characterized by synthesis of α1-acid glycoprotein that exhibits an immunomodulatory effect. in Journal of Applied Toxicology. 2008;28(1):63-71.
doi:10.1002/jat.1254 .

Ivanović-Matić, Svetlana, Poznanović, Goran, Grigorov, Ilijana, Dinić, Svetlana, Mihailović, Mirjana, Grdović, Nevena, Uskoković, Aleksandra, Martinović, Vesna, Arambašić Jovanović, Jelena, Petrović, Miodrag, Bogojević, Desanka, "The organophosphate-induced acute-phase response is characterized by synthesis of α1-acid glycoprotein that exhibits an immunomodulatory effect" in Journal of Applied Toxicology, 28, no. 1 (2008):63-71,
https://doi.org/10.1002/jat.1254 . .

TY  - CONF
AU  - Uskoković, Aleksandra
AU  - Mihailović, Mirjana
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Vidaković, Melita
AU  - Arambašić Jovanović, Jelena
AU  - Bogojević, Desanka
AU  - Ivanović Matić, Svetlana
AU  - Martinović, Vesna
AU  - Petrović, Miodrag
AU  - Poznanović, Goran
AU  - Grigorov, Ilijana
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6075
AB  - During  the  acute-phase  (AP)  response,  the synthesis  rate  and serum  concentration of
the AP protein a 2-macroglobulin (MG) increases in the rat. MG gene transcription  is
mainly  controlled  by the inflammatory  cytokine  IL-6 and  related  trans- factors,   the
Signal  Transducers  and  Activators  of  Transcription  (STAT)  and  CCAAT/Enhancer
Binding   Protein   (C/EBP) .  Nearly   every   aspect   of   the  body's  defense  system· is changed  in  malnutrition  (MN). We examined  whether  MN  affected  rat  MG  synthesis
and  STAT3  and  C/EBP  expression.  MN  was  induced  in  male  Wistar  rats .by 50%
food  restriction  during  six  weeks. The  AP  response was induced  by  turpentine oil injection (1 µl/g body weight). Using "rocket"  immuno electrophoresis we observed that,  compared   to   the  well-nourished   (WN)   control,  MN   was  associated  wit increased serum  MG. The  AP  response  promoted  a further rise  in  se rum  MG. The STAT3  and  C/EBP	levels  in  the  nuclear  extract  were  examined  by  immunoblote	analysis.  In  rats  with   MN,  STAT3  was  elevated  and  during  the   AP  response, increased  STAT3 levels  were observed  in both  WN and MN  rats. The C/EBP	levels were similar  in  MN  and  WN  rats  however  the  AP response  induced its significants, increase in rats with MN. Obtained results suggested that malnutrition contribute  to an  increased MG expression by  influencing  IL-6  signal  transduction  pathway.
Malnutrition-related increased of MG synthesis could be connected with positive regulatory role of STAT3 , while the additional AP-related MG increased synthesis in malnourished  rats require  activit y of C/EBP .















































•
111
PB  - Belgrade: Serbian Physiological Society
C3  - Abstract book: A Scientific Conference with International Participation: Nutrition, Treatment and Cardiovascular Risk Management; 2007 May 24-27; Novi Sad, Serbia
T1  - α2-macroglobulin expression during the acute-phase response in malnourished rats
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6075
ER  - 

@conference{
author = "Uskoković, Aleksandra and Mihailović, Mirjana and Dinić, Svetlana and Grdović, Nevena and Vidaković, Melita and Arambašić Jovanović, Jelena and Bogojević, Desanka and Ivanović Matić, Svetlana and Martinović, Vesna and Petrović, Miodrag and Poznanović, Goran and Grigorov, Ilijana",
year = "2007",
abstract = "During  the  acute-phase  (AP)  response,  the synthesis  rate  and serum  concentration of
the AP protein a 2-macroglobulin (MG) increases in the rat. MG gene transcription  is
mainly  controlled  by the inflammatory  cytokine  IL-6 and  related  trans- factors,   the
Signal  Transducers  and  Activators  of  Transcription  (STAT)  and  CCAAT/Enhancer
Binding   Protein   (C/EBP) .  Nearly   every   aspect   of   the  body's  defense  system· is changed  in  malnutrition  (MN). We examined  whether  MN  affected  rat  MG  synthesis
and  STAT3  and  C/EBP  expression.  MN  was  induced  in  male  Wistar  rats .by 50%
food  restriction  during  six  weeks. The  AP  response was induced  by  turpentine oil injection (1 µl/g body weight). Using "rocket"  immuno electrophoresis we observed that,  compared   to   the  well-nourished   (WN)   control,  MN   was  associated  wit increased serum  MG. The  AP  response  promoted  a further rise  in  se rum  MG. The STAT3  and  C/EBP	levels  in  the  nuclear  extract  were  examined  by  immunoblote	analysis.  In  rats  with   MN,  STAT3  was  elevated  and  during  the   AP  response, increased  STAT3 levels  were observed  in both  WN and MN  rats. The C/EBP	levels were similar  in  MN  and  WN  rats  however  the  AP response  induced its significants, increase in rats with MN. Obtained results suggested that malnutrition contribute  to an  increased MG expression by  influencing  IL-6  signal  transduction  pathway.
Malnutrition-related increased of MG synthesis could be connected with positive regulatory role of STAT3 , while the additional AP-related MG increased synthesis in malnourished  rats require  activit y of C/EBP .















































•
111",
publisher = "Belgrade: Serbian Physiological Society",
journal = "Abstract book: A Scientific Conference with International Participation: Nutrition, Treatment and Cardiovascular Risk Management; 2007 May 24-27; Novi Sad, Serbia",
title = "α2-macroglobulin expression during the acute-phase response in malnourished rats",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6075"
}

Uskoković, A., Mihailović, M., Dinić, S., Grdović, N., Vidaković, M., Arambašić Jovanović, J., Bogojević, D., Ivanović Matić, S., Martinović, V., Petrović, M., Poznanović, G.,& Grigorov, I.. (2007). α2-macroglobulin expression during the acute-phase response in malnourished rats. in Abstract book: A Scientific Conference with International Participation: Nutrition, Treatment and Cardiovascular Risk Management; 2007 May 24-27; Novi Sad, Serbia
Belgrade: Serbian Physiological Society., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_6075

Uskoković A, Mihailović M, Dinić S, Grdović N, Vidaković M, Arambašić Jovanović J, Bogojević D, Ivanović Matić S, Martinović V, Petrović M, Poznanović G, Grigorov I. α2-macroglobulin expression during the acute-phase response in malnourished rats. in Abstract book: A Scientific Conference with International Participation: Nutrition, Treatment and Cardiovascular Risk Management; 2007 May 24-27; Novi Sad, Serbia. 2007;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_6075 .

Uskoković, Aleksandra, Mihailović, Mirjana, Dinić, Svetlana, Grdović, Nevena, Vidaković, Melita, Arambašić Jovanović, Jelena, Bogojević, Desanka, Ivanović Matić, Svetlana, Martinović, Vesna, Petrović, Miodrag, Poznanović, Goran, Grigorov, Ilijana, "α2-macroglobulin expression during the acute-phase response in malnourished rats" in Abstract book: A Scientific Conference with International Participation: Nutrition, Treatment and Cardiovascular Risk Management; 2007 May 24-27; Novi Sad, Serbia (2007):111,
https://hdl.handle.net/21.15107/rcub_ibiss_6075 .

TY  - CONF
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Bogojević, Desanka
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Grigorov, Ilijana
AU  - Ivanović-Matić, Svetlana
AU  - Martinović, Vesna
AU  - Petrović, Miodrag
AU  - Uskoković, Aleksandra
AU  - Vidaković, Melita
AU  - Poznanović, Goran
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5656
AB  - In order to ,issess effects of environme ntal pollutants on aqu at ic ecosystem s, different biomarkers may be exa mined.  The  pha se  I  biotransforma t ion e nzymes, notab ly CYP l A, definitely belong to t he most sensitive fish biomarker s known at present. CYP l A is a well-established biomarker  of  exposure  of  fish  to  xenobiotic  compounds.  The  CY P l A  protein  le vels can  be  determined  immunologicaly  ,  using   mono-   or polyclonal  antibodies  with  ELISA,  Western-blotting   or   histochemic  al technique  s.  Generally,  a  good  correlation  is  observed  between  CYP I A mRNA , protein le ve l s and CYP I A activity.  ln  this  work  we  per fo rm ed Weste r-b lot analys is and our aim was to characterize the  in du c ti on  of  CYPIA in he patopanc reas of the  European  hake-Merluccius mer /ucc ius and Red mul let-Mu l/us barbatus and in  winter  and  spring  at  the  mouth  of  th e river  Bojana.  Both  species  are  of  considerable  commercial  import ance Whereas intersp ecies differences in protein profiles were established, no qualitative  or  quantitative  intraspecies  or  seasonal  variations  in  protein  profiles were observed. Western blot analysis with a polyclonal antibody to  CYP1A reve aled the CYP I A expression in both  examined  fish  species  in winter  at  the  very  low  level.   However,   the   relative   concent!"ations   of CYP I A were increased in sprin g. The apparent initiation of  the  defens e response in fish  suggests  that  the  contamin  ants  present  in  seawater  have  an eco toxico logic al potentia l. These findings could be interpreted as an ea rly­ warning  signal for the deleter ious effects  of  the  pollutants.  The  higher  le vel of CYP l A in spr in g could  be connecte d with increased antopo genic ac ti v ity at the mouth  of  the  river Bojana  in  this  part  of the year.
PB  - Novi Sad: Faculty of Sciences, Department of Biology and Ecology
C3  - Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
T1  - Seasonal depending expression of CYP1A in the hepatopancreas of Merluccius merluccius and Mullus barbatus at the mouth of the river Bojana
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5656
ER  - 

@conference{
author = "Mihailović, Mirjana and Arambašić Jovanović, Jelena and Bogojević, Desanka and Dinić, Svetlana and Grdović, Nevena and Grigorov, Ilijana and Ivanović-Matić, Svetlana and Martinović, Vesna and Petrović, Miodrag and Uskoković, Aleksandra and Vidaković, Melita and Poznanović, Goran",
year = "2007",
abstract = "In order to ,issess effects of environme ntal pollutants on aqu at ic ecosystem s, different biomarkers may be exa mined.  The  pha se  I  biotransforma t ion e nzymes, notab ly CYP l A, definitely belong to t he most sensitive fish biomarker s known at present. CYP l A is a well-established biomarker  of  exposure  of  fish  to  xenobiotic  compounds.  The  CY P l A  protein  le vels can  be  determined  immunologicaly  ,  using   mono-   or polyclonal  antibodies  with  ELISA,  Western-blotting   or   histochemic  al technique  s.  Generally,  a  good  correlation  is  observed  between  CYP I A mRNA , protein le ve l s and CYP I A activity.  ln  this  work  we  per fo rm ed Weste r-b lot analys is and our aim was to characterize the  in du c ti on  of  CYPIA in he patopanc reas of the  European  hake-Merluccius mer /ucc ius and Red mul let-Mu l/us barbatus and in  winter  and  spring  at  the  mouth  of  th e river  Bojana.  Both  species  are  of  considerable  commercial  import ance Whereas intersp ecies differences in protein profiles were established, no qualitative  or  quantitative  intraspecies  or  seasonal  variations  in  protein  profiles were observed. Western blot analysis with a polyclonal antibody to  CYP1A reve aled the CYP I A expression in both  examined  fish  species  in winter  at  the  very  low  level.   However,   the   relative   concent!"ations   of CYP I A were increased in sprin g. The apparent initiation of  the  defens e response in fish  suggests  that  the  contamin  ants  present  in  seawater  have  an eco toxico logic al potentia l. These findings could be interpreted as an ea rly­ warning  signal for the deleter ious effects  of  the  pollutants.  The  higher  le vel of CYP l A in spr in g could  be connecte d with increased antopo genic ac ti v ity at the mouth  of  the  river Bojana  in  this  part  of the year.",
publisher = "Novi Sad: Faculty of Sciences, Department of Biology and Ecology",
journal = "Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia",
title = "Seasonal depending expression of CYP1A in the hepatopancreas of Merluccius merluccius and Mullus barbatus at the mouth of the river Bojana",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5656"
}

Mihailović, M., Arambašić Jovanović, J., Bogojević, D., Dinić, S., Grdović, N., Grigorov, I., Ivanović-Matić, S., Martinović, V., Petrović, M., Uskoković, A., Vidaković, M.,& Poznanović, G.. (2007). Seasonal depending expression of CYP1A in the hepatopancreas of Merluccius merluccius and Mullus barbatus at the mouth of the river Bojana. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
Novi Sad: Faculty of Sciences, Department of Biology and Ecology..
https://hdl.handle.net/21.15107/rcub_ibiss_5656

Mihailović M, Arambašić Jovanović J, Bogojević D, Dinić S, Grdović N, Grigorov I, Ivanović-Matić S, Martinović V, Petrović M, Uskoković A, Vidaković M, Poznanović G. Seasonal depending expression of CYP1A in the hepatopancreas of Merluccius merluccius and Mullus barbatus at the mouth of the river Bojana. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia. 2007;.
https://hdl.handle.net/21.15107/rcub_ibiss_5656 .

Mihailović, Mirjana, Arambašić Jovanović, Jelena, Bogojević, Desanka, Dinić, Svetlana, Grdović, Nevena, Grigorov, Ilijana, Ivanović-Matić, Svetlana, Martinović, Vesna, Petrović, Miodrag, Uskoković, Aleksandra, Vidaković, Melita, Poznanović, Goran, "Seasonal depending expression of CYP1A in the hepatopancreas of Merluccius merluccius and Mullus barbatus at the mouth of the river Bojana" in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_5656 .

TY  - CONF
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Bogojević, Desanka
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Grigorov, Ilijana
AU  - Ivanović-Matić, Svetlana
AU  - Labus-Blagojević, Svetlana
AU  - Martinović, Vesna
AU  - Petrović, Miodrag
AU  - Uskoković, Aleksandra
AU  - Vidaković, Melita
AU  - Poznanović, Goran
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5637
AB  - The cytochrome P450 isozymes that are responsible for the biotransformation of xenobiotic compounds such as polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), are represented by the CYP1A subfamily of proteins in fish. CYP1A is well-established biomarker for environmental contamitantion in aquatic ecosystems. The aim of this study was to investigate CYP1A expression in the hepatic microsomal pellet of the European hake - Merluccius merluccius, the Tub gurnard - Trigla lucern and the Thinlip gray mullet Liza ramada during winter and spring in the sea-port Bar (Adriatic sea, Serbia and Montenegro), a region of intensive urban and industrial activities. Samples of sea water and sediment in this area were analysed for PAHs and PCBs. The presence of representative compounds from both groups was established in both seasons. Western blot analysis revealed the induction of CYP1A in all examined fish species, with increased relative amounts  of CYP1A in spring compared to winter. A species-specific response, characterized by the highest CYP1A level in the hepatopancreas of Liza ramada and the lowest level in Trigla lucerna was observed. Minimal concentrations of PAHs and PCBs in sea water that are considered capable of exerting a negative impact on marine organisms differ between countries. Therefore, the observed inducton of CYP1A could present a biological effect that links the bioavailability of the compounds of interest and their intrinsic toxicity. Measurements of biomarker responses in fish from contaminated sites could contribute to environmental monitoring and environmental risk assessment.
PB  - Podgorica: Centre for Biodiversity of Montenegro
C3  - The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro
T1  - CYP1A induction in the hepatopancreas of Merluccius merluccius, Trigla lucerna and Liza ramada in the region of the sea-port Bar
SP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5637
ER  - 

@conference{
author = "Mihailović, Mirjana and Arambašić Jovanović, Jelena and Bogojević, Desanka and Dinić, Svetlana and Grdović, Nevena and Grigorov, Ilijana and Ivanović-Matić, Svetlana and Labus-Blagojević, Svetlana and Martinović, Vesna and Petrović, Miodrag and Uskoković, Aleksandra and Vidaković, Melita and Poznanović, Goran",
year = "2007",
abstract = "The cytochrome P450 isozymes that are responsible for the biotransformation of xenobiotic compounds such as polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), are represented by the CYP1A subfamily of proteins in fish. CYP1A is well-established biomarker for environmental contamitantion in aquatic ecosystems. The aim of this study was to investigate CYP1A expression in the hepatic microsomal pellet of the European hake - Merluccius merluccius, the Tub gurnard - Trigla lucern and the Thinlip gray mullet Liza ramada during winter and spring in the sea-port Bar (Adriatic sea, Serbia and Montenegro), a region of intensive urban and industrial activities. Samples of sea water and sediment in this area were analysed for PAHs and PCBs. The presence of representative compounds from both groups was established in both seasons. Western blot analysis revealed the induction of CYP1A in all examined fish species, with increased relative amounts  of CYP1A in spring compared to winter. A species-specific response, characterized by the highest CYP1A level in the hepatopancreas of Liza ramada and the lowest level in Trigla lucerna was observed. Minimal concentrations of PAHs and PCBs in sea water that are considered capable of exerting a negative impact on marine organisms differ between countries. Therefore, the observed inducton of CYP1A could present a biological effect that links the bioavailability of the compounds of interest and their intrinsic toxicity. Measurements of biomarker responses in fish from contaminated sites could contribute to environmental monitoring and environmental risk assessment.",
publisher = "Podgorica: Centre for Biodiversity of Montenegro",
journal = "The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro",
title = "CYP1A induction in the hepatopancreas of Merluccius merluccius, Trigla lucerna and Liza ramada in the region of the sea-port Bar",
pages = "115",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5637"
}

Mihailović, M., Arambašić Jovanović, J., Bogojević, D., Dinić, S., Grdović, N., Grigorov, I., Ivanović-Matić, S., Labus-Blagojević, S., Martinović, V., Petrović, M., Uskoković, A., Vidaković, M.,& Poznanović, G.. (2007). CYP1A induction in the hepatopancreas of Merluccius merluccius, Trigla lucerna and Liza ramada in the region of the sea-port Bar. in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro
Podgorica: Centre for Biodiversity of Montenegro., 115.
https://hdl.handle.net/21.15107/rcub_ibiss_5637

Mihailović M, Arambašić Jovanović J, Bogojević D, Dinić S, Grdović N, Grigorov I, Ivanović-Matić S, Labus-Blagojević S, Martinović V, Petrović M, Uskoković A, Vidaković M, Poznanović G. CYP1A induction in the hepatopancreas of Merluccius merluccius, Trigla lucerna and Liza ramada in the region of the sea-port Bar. in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro. 2007;:115.
https://hdl.handle.net/21.15107/rcub_ibiss_5637 .

Mihailović, Mirjana, Arambašić Jovanović, Jelena, Bogojević, Desanka, Dinić, Svetlana, Grdović, Nevena, Grigorov, Ilijana, Ivanović-Matić, Svetlana, Labus-Blagojević, Svetlana, Martinović, Vesna, Petrović, Miodrag, Uskoković, Aleksandra, Vidaković, Melita, Poznanović, Goran, "CYP1A induction in the hepatopancreas of Merluccius merluccius, Trigla lucerna and Liza ramada in the region of the sea-port Bar" in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro (2007):115,
https://hdl.handle.net/21.15107/rcub_ibiss_5637 .

TY  - CONF
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Ivanović-Matić, Svetlana
AU  - Petrović, Miodrag
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Mihailović, Mirjana
AU  - Grdović, Nevena
AU  - Martinović, Vesna
AU  - Vidaković, Melita
AU  - Poznanović, Goran
AU  - Dinić, Svetlana
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5655
AB  - Homeostasis is the state of metabolic equilibrium supporting the optimal functioning of an organism that is actively maintained by complex biological mechanisms. An organism responds to deviations from this stable situation by a coordinated sequence of systemic and metabolic changes, or by local changes such as inflammatory reactions, collectively known as the acute phase response (APR) The APR is an evolutionarily conserved mechanism aimed at reestablishing an organisms normal functioning during infection, tissue injury, chemical irritation and neoplastic disease. The initiation and progression of the APR involves a coordinated series of events, including cytokine release, endothelial-cell activation, leukocyte chemotaxis, alteration of the temperature set-point and activation of the adrenal pituitary axis which provide the necessary metabolic signals to the responding organ . The liver is a 1 major partner in APR and the blood-borne inflammatory cytokines such as Tumor Necrosis Factor, lnterleukin-1and Interleukin-6 trigger transient changes in the synthesis of numerous proteins in this organ.
PB  - Novi Sad: Faculty of Sciences, Department of Biology and Ecology
C3  - Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
T1  - Molecular mechanisms of eucaryotic cell perception of and response to environmental signals
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5655
ER  - 

@conference{
author = "Grigorov, Ilijana and Bogojević, Desanka and Ivanović-Matić, Svetlana and Petrović, Miodrag and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Mihailović, Mirjana and Grdović, Nevena and Martinović, Vesna and Vidaković, Melita and Poznanović, Goran and Dinić, Svetlana",
year = "2007",
abstract = "Homeostasis is the state of metabolic equilibrium supporting the optimal functioning of an organism that is actively maintained by complex biological mechanisms. An organism responds to deviations from this stable situation by a coordinated sequence of systemic and metabolic changes, or by local changes such as inflammatory reactions, collectively known as the acute phase response (APR) The APR is an evolutionarily conserved mechanism aimed at reestablishing an organisms normal functioning during infection, tissue injury, chemical irritation and neoplastic disease. The initiation and progression of the APR involves a coordinated series of events, including cytokine release, endothelial-cell activation, leukocyte chemotaxis, alteration of the temperature set-point and activation of the adrenal pituitary axis which provide the necessary metabolic signals to the responding organ . The liver is a 1 major partner in APR and the blood-borne inflammatory cytokines such as Tumor Necrosis Factor, lnterleukin-1and Interleukin-6 trigger transient changes in the synthesis of numerous proteins in this organ.",
publisher = "Novi Sad: Faculty of Sciences, Department of Biology and Ecology",
journal = "Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia",
title = "Molecular mechanisms of eucaryotic cell perception of and response to environmental signals",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5655"
}

Grigorov, I., Bogojević, D., Ivanović-Matić, S., Petrović, M., Uskoković, A., Arambašić Jovanović, J., Mihailović, M., Grdović, N., Martinović, V., Vidaković, M., Poznanović, G.,& Dinić, S.. (2007). Molecular mechanisms of eucaryotic cell perception of and response to environmental signals. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
Novi Sad: Faculty of Sciences, Department of Biology and Ecology..
https://hdl.handle.net/21.15107/rcub_ibiss_5655

Grigorov I, Bogojević D, Ivanović-Matić S, Petrović M, Uskoković A, Arambašić Jovanović J, Mihailović M, Grdović N, Martinović V, Vidaković M, Poznanović G, Dinić S. Molecular mechanisms of eucaryotic cell perception of and response to environmental signals. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia. 2007;.
https://hdl.handle.net/21.15107/rcub_ibiss_5655 .

Grigorov, Ilijana, Bogojević, Desanka, Ivanović-Matić, Svetlana, Petrović, Miodrag, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Mihailović, Mirjana, Grdović, Nevena, Martinović, Vesna, Vidaković, Melita, Poznanović, Goran, Dinić, Svetlana, "Molecular mechanisms of eucaryotic cell perception of and response to environmental signals" in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_5655 .

TY  - CONF
AU  - Grigorov, Ilijana
AU  - Arambašić Jovanović, Jelena
AU  - Bogojević, Desanka
AU  - Ivanović-Matić, Svetlana
AU  - Grdović, Nevena
AU  - Vidaković, Melita
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Martinović, Vesna
AU  - Petrović, Miodrag
AU  - Poznanović, Goran
AU  - Mihailović, Mirjana
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5603
AB  - Oxidative stress was proposed as a key factor in the development of complications in diabetes. Antioxidant defense mechanisms pri- marly act through a broad spectrum of antioxidative enzymes, how- ever, other molecules could also express antioxidative properties. One such molecule is haptoglobin (Hp), a serum protein that func- tions as an antioxidant by virtue of its ability to bind hemoglobin and thereby prevent oxidative tissue damage. Elevation of serum Hp con- centration was obtained 12 h after acute tissue injury, and its level decreased after 24h to the control level. In our studies of streptozo- tocin-induced diabetes in adult male Wistar rats (i.p., 65mg/kg body weight), we found chronic but different degrees of increased serum Hp concentrations depending on time after treatment. The highest elevation (eight-fold), was obtained two weeks after treatment by rocket immunoelectrophoresys where the maximal activation of total SOD and superoxide anion was detected (ELISA) in the blood and in the liver, the central organ of Hp synthesis. At this time, most of the diabetic rats died suggesting that the early stage of diabetes was critical for survival. Four end seven weeks after treatment, expres- sion of Hp was still elevated, but less than after two weeks, as was the concentration of the superoxide anion and SOD activity. The strong correlation between the diabetes-related oxidative status and expression of Hp suggests that its antioxidative property plays an important role in the early stage of diabetes.
PB  - SAGE Publications
C3  - Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain
T1  - Antioxidant properties of haptoglobin in experimental diabetes
SP  - S176
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5603
ER  - 

@conference{
author = "Grigorov, Ilijana and Arambašić Jovanović, Jelena and Bogojević, Desanka and Ivanović-Matić, Svetlana and Grdović, Nevena and Vidaković, Melita and Dinić, Svetlana and Uskoković, Aleksandra and Martinović, Vesna and Petrović, Miodrag and Poznanović, Goran and Mihailović, Mirjana",
year = "2007",
abstract = "Oxidative stress was proposed as a key factor in the development of complications in diabetes. Antioxidant defense mechanisms pri- marly act through a broad spectrum of antioxidative enzymes, how- ever, other molecules could also express antioxidative properties. One such molecule is haptoglobin (Hp), a serum protein that func- tions as an antioxidant by virtue of its ability to bind hemoglobin and thereby prevent oxidative tissue damage. Elevation of serum Hp con- centration was obtained 12 h after acute tissue injury, and its level decreased after 24h to the control level. In our studies of streptozo- tocin-induced diabetes in adult male Wistar rats (i.p., 65mg/kg body weight), we found chronic but different degrees of increased serum Hp concentrations depending on time after treatment. The highest elevation (eight-fold), was obtained two weeks after treatment by rocket immunoelectrophoresys where the maximal activation of total SOD and superoxide anion was detected (ELISA) in the blood and in the liver, the central organ of Hp synthesis. At this time, most of the diabetic rats died suggesting that the early stage of diabetes was critical for survival. Four end seven weeks after treatment, expres- sion of Hp was still elevated, but less than after two weeks, as was the concentration of the superoxide anion and SOD activity. The strong correlation between the diabetes-related oxidative status and expression of Hp suggests that its antioxidative property plays an important role in the early stage of diabetes.",
publisher = "SAGE Publications",
journal = "Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain",
title = "Antioxidant properties of haptoglobin in experimental diabetes",
pages = "S176",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5603"
}

Grigorov, I., Arambašić Jovanović, J., Bogojević, D., Ivanović-Matić, S., Grdović, N., Vidaković, M., Dinić, S., Uskoković, A., Martinović, V., Petrović, M., Poznanović, G.,& Mihailović, M.. (2007). Antioxidant properties of haptoglobin in experimental diabetes. in Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain
SAGE Publications., S176.
https://hdl.handle.net/21.15107/rcub_ibiss_5603

Grigorov I, Arambašić Jovanović J, Bogojević D, Ivanović-Matić S, Grdović N, Vidaković M, Dinić S, Uskoković A, Martinović V, Petrović M, Poznanović G, Mihailović M. Antioxidant properties of haptoglobin in experimental diabetes. in Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain. 2007;:S176.
https://hdl.handle.net/21.15107/rcub_ibiss_5603 .

Grigorov, Ilijana, Arambašić Jovanović, Jelena, Bogojević, Desanka, Ivanović-Matić, Svetlana, Grdović, Nevena, Vidaković, Melita, Dinić, Svetlana, Uskoković, Aleksandra, Martinović, Vesna, Petrović, Miodrag, Poznanović, Goran, Mihailović, Mirjana, "Antioxidant properties of haptoglobin in experimental diabetes" in Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain (2007):S176,
https://hdl.handle.net/21.15107/rcub_ibiss_5603 .

TY  - CONF
AU  - Bogojević, Desanka
AU  - Mihailović, Mirjana
AU  - Poznanović, Goran
AU  - Ivanović-Matić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Dinić, Svetlana
AU  - Arambašić Jovanović, Jelena
AU  - Martinović, Vesna
AU  - Vidaković, Melita
AU  - Petrović, Miodrag
AU  - Grdović, Nevena
AU  - Grigorov, Ilijana
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5602
AB  - The serum concentrations of several acute-phase proteins are increased during diabetes mellitus as a consequence of the activity of the main inflammatory mediators of the acute-phase response. The diabetic state leads to themetabolic syndrome, characterized by many negative effects. Our study was focused on ·2-macroglobulin (MG), one of the most prominent acute-phase proteins in the rat. Changes in MG concentration were examined in the laboratory model ofstrepto- zotocin-induced (65 mg/kg body weight) diabetes in the rat. Rocket immunoelectrophoresis revealed increased MG concentration by the 7th week after diabetes induction. During the period leading up to the 7th week, the observed elevated concentrations of triglycerides and cortisol were symptomatic of the progression of the metabolic syn- drome. Previously, we showed that MG exerts an immunosuppressive effect.Whereas during acute stressMGplays a role in restoring home- ostasis, its raised concentration during protracted stress exerts a harm- ful effect; moreover,MGis an established biomarker of cardiac hyper- trophy in diabetesmellitus.
PB  - SAGE Publications
C3  - Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain
T1  - Alpha2-macroglobulin in streptozotocin-treated rats
DO  - 10.3132/dvdr.2007.012
SP  - S186
ER  - 

@conference{
author = "Bogojević, Desanka and Mihailović, Mirjana and Poznanović, Goran and Ivanović-Matić, Svetlana and Uskoković, Aleksandra and Dinić, Svetlana and Arambašić Jovanović, Jelena and Martinović, Vesna and Vidaković, Melita and Petrović, Miodrag and Grdović, Nevena and Grigorov, Ilijana",
year = "2007",
abstract = "The serum concentrations of several acute-phase proteins are increased during diabetes mellitus as a consequence of the activity of the main inflammatory mediators of the acute-phase response. The diabetic state leads to themetabolic syndrome, characterized by many negative effects. Our study was focused on ·2-macroglobulin (MG), one of the most prominent acute-phase proteins in the rat. Changes in MG concentration were examined in the laboratory model ofstrepto- zotocin-induced (65 mg/kg body weight) diabetes in the rat. Rocket immunoelectrophoresis revealed increased MG concentration by the 7th week after diabetes induction. During the period leading up to the 7th week, the observed elevated concentrations of triglycerides and cortisol were symptomatic of the progression of the metabolic syn- drome. Previously, we showed that MG exerts an immunosuppressive effect.Whereas during acute stressMGplays a role in restoring home- ostasis, its raised concentration during protracted stress exerts a harm- ful effect; moreover,MGis an established biomarker of cardiac hyper- trophy in diabetesmellitus.",
publisher = "SAGE Publications",
journal = "Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain",
title = "Alpha2-macroglobulin in streptozotocin-treated rats",
doi = "10.3132/dvdr.2007.012",
pages = "S186"
}

Bogojević, D., Mihailović, M., Poznanović, G., Ivanović-Matić, S., Uskoković, A., Dinić, S., Arambašić Jovanović, J., Martinović, V., Vidaković, M., Petrović, M., Grdović, N.,& Grigorov, I.. (2007). Alpha2-macroglobulin in streptozotocin-treated rats. in Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain
SAGE Publications., S186.
https://doi.org/10.3132/dvdr.2007.012

Bogojević D, Mihailović M, Poznanović G, Ivanović-Matić S, Uskoković A, Dinić S, Arambašić Jovanović J, Martinović V, Vidaković M, Petrović M, Grdović N, Grigorov I. Alpha2-macroglobulin in streptozotocin-treated rats. in Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain. 2007;:S186.
doi:10.3132/dvdr.2007.012 .

Bogojević, Desanka, Mihailović, Mirjana, Poznanović, Goran, Ivanović-Matić, Svetlana, Uskoković, Aleksandra, Dinić, Svetlana, Arambašić Jovanović, Jelena, Martinović, Vesna, Vidaković, Melita, Petrović, Miodrag, Grdović, Nevena, Grigorov, Ilijana, "Alpha2-macroglobulin in streptozotocin-treated rats" in Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain (2007):S186,
https://doi.org/10.3132/dvdr.2007.012 . .

TY  - JOUR
AU  - Mihailović, Mirjana
AU  - Dinić, Svetlana
AU  - Bogojević, Desanka
AU  - Ivanović-Matić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Grigorov, Ilijana
AU  - Grdović, Nevena
AU  - Vidaković, Melita
AU  - Martinović, Vesna
AU  - Petrović, Miodrag
AU  - Poznanović, Goran
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5545
AB  - Expression of the rat α2-macroglobulin (MG) gene undergoes dynamic changes throughout an individual’s
life and during the acute-phase (AP) response. Details of the participation of the STAT
family of transcription factors in its control remain incompletely understood. Here we examined
the involvement of STAT5b in MG gene expression during development and the AP response.
Immuno-blot analysis revealed the highest nuclear level of STAT5b in the fetus and during postnatal
development, whereas in the adult it decreased. Stimulation of MG expression during the
AP response was accompanied by a decrease in STAT5b. Examination of STAT5b localization
revealed that the relative concentrations of STAT5b were higher in the nuclear matrix than in
the nuclear extract. Affinity chromatography with the extended promoter region of the MG gene
(–825/+12), followed by immuno-blot analysis, revealed dynamic changes in STAT5b binding.
The highest concentration of the promoter-binding form of STAT5b was observed in the fetus.
As postnatal development progressed, the level of promoter-bound STAT5b decreased and in
the adult liver it was the lowest. Stimulation of MG gene expression during the AP response
in both the fetus and adult was accompanied by significantly decreased STAT5b binding to the
MG promoter. The AP response was accompanied by lower levels of STAT5b serine and tyrosine
phosphorylation in both fetus and adult. In the nuclear matrix derived from adult tissues, tyrosine
phosphorylated species were completely absent. We conclude that developmental-stage differences
in the mechanisms that determine STAT5b nuclear localization contribute to its activity
in vivo.
PB  - Warsaw: Acta Biochimica Polonica
T2  - Acta Biochimica Polonica
T1  - Nuclear localization and binding affinity of STAT5b for the alpha2-macroglobulin gene promoter during rat liver development and the acute-phase response
IS  - 2
VL  - 54
DO  - 10.18388/abp.2007_3254
SP  - 331
EP  - 340
ER  - 

@article{
author = "Mihailović, Mirjana and Dinić, Svetlana and Bogojević, Desanka and Ivanović-Matić, Svetlana and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Grigorov, Ilijana and Grdović, Nevena and Vidaković, Melita and Martinović, Vesna and Petrović, Miodrag and Poznanović, Goran",
year = "2007",
abstract = "Expression of the rat α2-macroglobulin (MG) gene undergoes dynamic changes throughout an individual’s
life and during the acute-phase (AP) response. Details of the participation of the STAT
family of transcription factors in its control remain incompletely understood. Here we examined
the involvement of STAT5b in MG gene expression during development and the AP response.
Immuno-blot analysis revealed the highest nuclear level of STAT5b in the fetus and during postnatal
development, whereas in the adult it decreased. Stimulation of MG expression during the
AP response was accompanied by a decrease in STAT5b. Examination of STAT5b localization
revealed that the relative concentrations of STAT5b were higher in the nuclear matrix than in
the nuclear extract. Affinity chromatography with the extended promoter region of the MG gene
(–825/+12), followed by immuno-blot analysis, revealed dynamic changes in STAT5b binding.
The highest concentration of the promoter-binding form of STAT5b was observed in the fetus.
As postnatal development progressed, the level of promoter-bound STAT5b decreased and in
the adult liver it was the lowest. Stimulation of MG gene expression during the AP response
in both the fetus and adult was accompanied by significantly decreased STAT5b binding to the
MG promoter. The AP response was accompanied by lower levels of STAT5b serine and tyrosine
phosphorylation in both fetus and adult. In the nuclear matrix derived from adult tissues, tyrosine
phosphorylated species were completely absent. We conclude that developmental-stage differences
in the mechanisms that determine STAT5b nuclear localization contribute to its activity
in vivo.",
publisher = "Warsaw: Acta Biochimica Polonica",
journal = "Acta Biochimica Polonica",
title = "Nuclear localization and binding affinity of STAT5b for the alpha2-macroglobulin gene promoter during rat liver development and the acute-phase response",
number = "2",
volume = "54",
doi = "10.18388/abp.2007_3254",
pages = "331-340"
}

Mihailović, M., Dinić, S., Bogojević, D., Ivanović-Matić, S., Uskoković, A., Arambašić Jovanović, J., Grigorov, I., Grdović, N., Vidaković, M., Martinović, V., Petrović, M.,& Poznanović, G.. (2007). Nuclear localization and binding affinity of STAT5b for the alpha2-macroglobulin gene promoter during rat liver development and the acute-phase response. in Acta Biochimica Polonica
Warsaw: Acta Biochimica Polonica., 54(2), 331-340.
https://doi.org/10.18388/abp.2007_3254

Mihailović M, Dinić S, Bogojević D, Ivanović-Matić S, Uskoković A, Arambašić Jovanović J, Grigorov I, Grdović N, Vidaković M, Martinović V, Petrović M, Poznanović G. Nuclear localization and binding affinity of STAT5b for the alpha2-macroglobulin gene promoter during rat liver development and the acute-phase response. in Acta Biochimica Polonica. 2007;54(2):331-340.
doi:10.18388/abp.2007_3254 .

Mihailović, Mirjana, Dinić, Svetlana, Bogojević, Desanka, Ivanović-Matić, Svetlana, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Grigorov, Ilijana, Grdović, Nevena, Vidaković, Melita, Martinović, Vesna, Petrović, Miodrag, Poznanović, Goran, "Nuclear localization and binding affinity of STAT5b for the alpha2-macroglobulin gene promoter during rat liver development and the acute-phase response" in Acta Biochimica Polonica, 54, no. 2 (2007):331-340,
https://doi.org/10.18388/abp.2007_3254 . .

TY  - JOUR
AU  - Grdović, Nevena
AU  - Mihailović, Mirjana
AU  - Vidaković, Melita
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Martinović, Vesna
AU  - Arambašić Jovanović, Jelena
AU  - Grigorov, Ilijana
AU  - Ivanović-Matić, Svetlana
AU  - Bogojević, Desanka
AU  - Petrović, Miodrag
AU  - Poznanović, Goran
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5544
AB  - Previously, we characterized the endonucleolytic activity of the nuclear matrix prepared from rat liver cryopreserved in liquid
nitrogen. The enzymic activity was attributed to a 23 kDa, Mg2þ
-dependent and sequence non-specific endonuclease (p23)
stably associated with the nuclear matrix. Here we show that p23 was absent from the nuclear matrix prepared from fresh
liver. Instead, both ex vivo (cryopreservation), as well as in vivo-induced necrosis by repeated freezing/thawing of liver tissue
in an anaesthetized rat, promoted the activation and translocation of p23 to the nuclear matrix. Considering that ex vivo and
in vivo freezing/thawing of the liver were accompanied by morphological (nuclear compaction) and biochemical events
(increased LDH activity, disorderly genomic DNA degradation, absence of lamin proteolysis, appearance of 62 and 50 kDa
necrotic cleavage products of PARP-1) commonly observed during necrosis, and because the association of p23 with the
nuclear matrix was saturable, reflecting the existence of a limited number of distinct high affinity sites on the nuclear matrix
for p23, we concluded that the activation of the nuclear matrix-associated endonuclease p23 is a feature of liver cryonecrosis.
Although cryonecrosis represents a typical example of acute cell damage, our results suggest that it is realized by ordered
molecular events.
PB  - Hoboken : John Wiley & Sons Ltd
T2  - Cell Biochemistry and Function
T1  - Establishment of association of an Mg2+-dependent endonuclease with the rat liver nuclear matrix in cryonecrosis
IS  - 3
VL  - 25
DO  - 10.1002/cbf.1407
SP  - 345
EP  - 355
ER  - 

@article{
author = "Grdović, Nevena and Mihailović, Mirjana and Vidaković, Melita and Dinić, Svetlana and Uskoković, Aleksandra and Martinović, Vesna and Arambašić Jovanović, Jelena and Grigorov, Ilijana and Ivanović-Matić, Svetlana and Bogojević, Desanka and Petrović, Miodrag and Poznanović, Goran",
year = "2007",
abstract = "Previously, we characterized the endonucleolytic activity of the nuclear matrix prepared from rat liver cryopreserved in liquid
nitrogen. The enzymic activity was attributed to a 23 kDa, Mg2þ
-dependent and sequence non-specific endonuclease (p23)
stably associated with the nuclear matrix. Here we show that p23 was absent from the nuclear matrix prepared from fresh
liver. Instead, both ex vivo (cryopreservation), as well as in vivo-induced necrosis by repeated freezing/thawing of liver tissue
in an anaesthetized rat, promoted the activation and translocation of p23 to the nuclear matrix. Considering that ex vivo and
in vivo freezing/thawing of the liver were accompanied by morphological (nuclear compaction) and biochemical events
(increased LDH activity, disorderly genomic DNA degradation, absence of lamin proteolysis, appearance of 62 and 50 kDa
necrotic cleavage products of PARP-1) commonly observed during necrosis, and because the association of p23 with the
nuclear matrix was saturable, reflecting the existence of a limited number of distinct high affinity sites on the nuclear matrix
for p23, we concluded that the activation of the nuclear matrix-associated endonuclease p23 is a feature of liver cryonecrosis.
Although cryonecrosis represents a typical example of acute cell damage, our results suggest that it is realized by ordered
molecular events.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "Cell Biochemistry and Function",
title = "Establishment of association of an Mg2+-dependent endonuclease with the rat liver nuclear matrix in cryonecrosis",
number = "3",
volume = "25",
doi = "10.1002/cbf.1407",
pages = "345-355"
}

Grdović, N., Mihailović, M., Vidaković, M., Dinić, S., Uskoković, A., Martinović, V., Arambašić Jovanović, J., Grigorov, I., Ivanović-Matić, S., Bogojević, D., Petrović, M.,& Poznanović, G.. (2007). Establishment of association of an Mg2+-dependent endonuclease with the rat liver nuclear matrix in cryonecrosis. in Cell Biochemistry and Function
Hoboken : John Wiley & Sons Ltd., 25(3), 345-355.
https://doi.org/10.1002/cbf.1407

Grdović N, Mihailović M, Vidaković M, Dinić S, Uskoković A, Martinović V, Arambašić Jovanović J, Grigorov I, Ivanović-Matić S, Bogojević D, Petrović M, Poznanović G. Establishment of association of an Mg2+-dependent endonuclease with the rat liver nuclear matrix in cryonecrosis. in Cell Biochemistry and Function. 2007;25(3):345-355.
doi:10.1002/cbf.1407 .

Grdović, Nevena, Mihailović, Mirjana, Vidaković, Melita, Dinić, Svetlana, Uskoković, Aleksandra, Martinović, Vesna, Arambašić Jovanović, Jelena, Grigorov, Ilijana, Ivanović-Matić, Svetlana, Bogojević, Desanka, Petrović, Miodrag, Poznanović, Goran, "Establishment of association of an Mg2+-dependent endonuclease with the rat liver nuclear matrix in cryonecrosis" in Cell Biochemistry and Function, 25, no. 3 (2007):345-355,
https://doi.org/10.1002/cbf.1407 . .

TY  - CONF
AU  - Dinić, Svetlana
AU  - Arambašić Jovanović, Jelena
AU  - Bogojević, Desanka
AU  - Grdović, Nevena
AU  - Grigorov, Ilijana
AU  - Ivanović-Matić, Svetlana
AU  - Labus-Blagojević, Svetlana
AU  - Martinović, Vesna
AU  - Mihailović, Mirjana
AU  - Petrović, Miodrag
AU  - Uskoković, Aleksandra
AU  - Vidaković, Melita
AU  - Poznanović, Goran
PY  - 2006
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5636
AB  - CYP1A is a well-established biomarker of fish exposure to xenobiotics. Polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) are ubiquitous environmental pollutants in the aquatic environment. They induce a dose-dependent transcriptional induction of CYP1A genes and resulting increased concentrations of proteins. Our aim was to characterize the ecological impact of chemical contaminants in view of their toxicological potential. For that reasons the relative changes of CYP1A concentrations in the hepatopancreas of the Red mullet-Mullus barbatus, European hake-Merluccius merluccius and Tub gurnard-Trigla lucerna were examined in spring, at the mouth of the river Bojana. All species are of considerable commercial importance. In this work chemical analysis of seawater was performed and CYP1A induction was estimated by immunoblot analysis. Chemical analyses of seawater and sediment revealed the presence of anthracene, benzo(A)pyrene, pcb28 and pcb101. CYP1A was detected in the hepatic microsomal fraction in all examined fish species as an adaptive response to the slightly increased concentrations of PAHs and PCBs. It was shown in this study that CYP1A is a sensitive biomarker demonstrating that pollutants entered the organisms and elicited toxic effects at the critical target(s). The apparent initiation of the defense response in fish suggests that the contaminants present in seawater have an ecotoxicological potential. These findings could be interpreted as an early-warning signal for the deleterious effects of the pollutants.
PB  - Podgorica: Centre for Biodiversity of Montenegro
C3  - The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro
T1  - The expression of CYP1A in the hepatopanceas of analysed fish species at the mouth of the river Bojana
SP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5636
ER  - 

@conference{
author = "Dinić, Svetlana and Arambašić Jovanović, Jelena and Bogojević, Desanka and Grdović, Nevena and Grigorov, Ilijana and Ivanović-Matić, Svetlana and Labus-Blagojević, Svetlana and Martinović, Vesna and Mihailović, Mirjana and Petrović, Miodrag and Uskoković, Aleksandra and Vidaković, Melita and Poznanović, Goran",
year = "2006",
abstract = "CYP1A is a well-established biomarker of fish exposure to xenobiotics. Polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) are ubiquitous environmental pollutants in the aquatic environment. They induce a dose-dependent transcriptional induction of CYP1A genes and resulting increased concentrations of proteins. Our aim was to characterize the ecological impact of chemical contaminants in view of their toxicological potential. For that reasons the relative changes of CYP1A concentrations in the hepatopancreas of the Red mullet-Mullus barbatus, European hake-Merluccius merluccius and Tub gurnard-Trigla lucerna were examined in spring, at the mouth of the river Bojana. All species are of considerable commercial importance. In this work chemical analysis of seawater was performed and CYP1A induction was estimated by immunoblot analysis. Chemical analyses of seawater and sediment revealed the presence of anthracene, benzo(A)pyrene, pcb28 and pcb101. CYP1A was detected in the hepatic microsomal fraction in all examined fish species as an adaptive response to the slightly increased concentrations of PAHs and PCBs. It was shown in this study that CYP1A is a sensitive biomarker demonstrating that pollutants entered the organisms and elicited toxic effects at the critical target(s). The apparent initiation of the defense response in fish suggests that the contaminants present in seawater have an ecotoxicological potential. These findings could be interpreted as an early-warning signal for the deleterious effects of the pollutants.",
publisher = "Podgorica: Centre for Biodiversity of Montenegro",
journal = "The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro",
title = "The expression of CYP1A in the hepatopanceas of analysed fish species at the mouth of the river Bojana",
pages = "115",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5636"
}

Dinić, S., Arambašić Jovanović, J., Bogojević, D., Grdović, N., Grigorov, I., Ivanović-Matić, S., Labus-Blagojević, S., Martinović, V., Mihailović, M., Petrović, M., Uskoković, A., Vidaković, M.,& Poznanović, G.. (2006). The expression of CYP1A in the hepatopanceas of analysed fish species at the mouth of the river Bojana. in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro
Podgorica: Centre for Biodiversity of Montenegro., 115.
https://hdl.handle.net/21.15107/rcub_ibiss_5636

Dinić S, Arambašić Jovanović J, Bogojević D, Grdović N, Grigorov I, Ivanović-Matić S, Labus-Blagojević S, Martinović V, Mihailović M, Petrović M, Uskoković A, Vidaković M, Poznanović G. The expression of CYP1A in the hepatopanceas of analysed fish species at the mouth of the river Bojana. in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro. 2006;:115.
https://hdl.handle.net/21.15107/rcub_ibiss_5636 .

Dinić, Svetlana, Arambašić Jovanović, Jelena, Bogojević, Desanka, Grdović, Nevena, Grigorov, Ilijana, Ivanović-Matić, Svetlana, Labus-Blagojević, Svetlana, Martinović, Vesna, Mihailović, Mirjana, Petrović, Miodrag, Uskoković, Aleksandra, Vidaković, Melita, Poznanović, Goran, "The expression of CYP1A in the hepatopanceas of analysed fish species at the mouth of the river Bojana" in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro (2006):115,
https://hdl.handle.net/21.15107/rcub_ibiss_5636 .