TY  - JOUR
AU  - Grbović, Filip
AU  - Gajić, Gordana
AU  - Branković, Snezana
AU  - Simić, Zoran
AU  - Vuković, Nenad
AU  - Pavlović, Pavle
AU  - Topuzović, Marina
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0352-51391900062G
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3721
AB  - Asbestos is widely mined and used around the globe posing a great risk to environment and human health. The main objective of this study was to determine allelopathic potential of Robinia pseudoacacia L. and Ailanthus altissima (Mill.) Swingle growing on the asbestos deposits at abandoned mine “Stragari” in central Serbia. The pH, content of carbon, nitrogen, calcium car­bonate, available phosphorous and potassium, content of Fe, Ni, Cu, Zn, Pb, Mn, and phenolics were analyzed in the control asbestos (zones without vege­tation cover) and plant rhizospheric asbestos. Allelopathic activity of plant species was assessed by “rhizosphere soil method”, and Trifolium pratense L. and Medicago sativa L. were used as the indicator species. A. altissima showed higher allelopathic potential compared to R. pseudoacacia for T. pratense and M. sativa due to greater content of phenolics. Alleopathic activity of phenolics in rhizospheric asbestos was highly correlated with pH, content of carbon and nitrogen, available phosphate and potassium, and content of Ni, Cu, Zn, Pb and Mn. A. altissima increased phenolics content in rhizospheric asbestos inhi­biting the plant growth. This woody plant in spite of high allelopathic potential is suitable for revegetation of distrurbed ecosystems because it initiates pedo­gen­esis and affects the asbestos chemistry.
T2  - Journal of the Serbian Chemical Society
T1  - Complex effect of Robinia pseudoacacia L. and Ailanthus altissima (Mill.) Swingle growing on asbestos deposits: Allelopathy and biogeochemistry
IS  - 1
VL  - 85
DO  - 10.2298/JSC190416062G
SP  - 141
EP  - 153
ER  - 

@article{
author = "Grbović, Filip and Gajić, Gordana and Branković, Snezana and Simić, Zoran and Vuković, Nenad and Pavlović, Pavle and Topuzović, Marina",
year = "2020",
abstract = "Asbestos is widely mined and used around the globe posing a great risk to environment and human health. The main objective of this study was to determine allelopathic potential of Robinia pseudoacacia L. and Ailanthus altissima (Mill.) Swingle growing on the asbestos deposits at abandoned mine “Stragari” in central Serbia. The pH, content of carbon, nitrogen, calcium car­bonate, available phosphorous and potassium, content of Fe, Ni, Cu, Zn, Pb, Mn, and phenolics were analyzed in the control asbestos (zones without vege­tation cover) and plant rhizospheric asbestos. Allelopathic activity of plant species was assessed by “rhizosphere soil method”, and Trifolium pratense L. and Medicago sativa L. were used as the indicator species. A. altissima showed higher allelopathic potential compared to R. pseudoacacia for T. pratense and M. sativa due to greater content of phenolics. Alleopathic activity of phenolics in rhizospheric asbestos was highly correlated with pH, content of carbon and nitrogen, available phosphate and potassium, and content of Ni, Cu, Zn, Pb and Mn. A. altissima increased phenolics content in rhizospheric asbestos inhi­biting the plant growth. This woody plant in spite of high allelopathic potential is suitable for revegetation of distrurbed ecosystems because it initiates pedo­gen­esis and affects the asbestos chemistry.",
journal = "Journal of the Serbian Chemical Society",
title = "Complex effect of Robinia pseudoacacia L. and Ailanthus altissima (Mill.) Swingle growing on asbestos deposits: Allelopathy and biogeochemistry",
number = "1",
volume = "85",
doi = "10.2298/JSC190416062G",
pages = "141-153"
}

Grbović, F., Gajić, G., Branković, S., Simić, Z., Vuković, N., Pavlović, P.,& Topuzović, M.. (2020). Complex effect of Robinia pseudoacacia L. and Ailanthus altissima (Mill.) Swingle growing on asbestos deposits: Allelopathy and biogeochemistry. in Journal of the Serbian Chemical Society, 85(1), 141-153.
https://doi.org/10.2298/JSC190416062G

Grbović F, Gajić G, Branković S, Simić Z, Vuković N, Pavlović P, Topuzović M. Complex effect of Robinia pseudoacacia L. and Ailanthus altissima (Mill.) Swingle growing on asbestos deposits: Allelopathy and biogeochemistry. in Journal of the Serbian Chemical Society. 2020;85(1):141-153.
doi:10.2298/JSC190416062G .

Grbović, Filip, Gajić, Gordana, Branković, Snezana, Simić, Zoran, Vuković, Nenad, Pavlović, Pavle, Topuzović, Marina, "Complex effect of Robinia pseudoacacia L. and Ailanthus altissima (Mill.) Swingle growing on asbestos deposits: Allelopathy and biogeochemistry" in Journal of the Serbian Chemical Society, 85, no. 1 (2020):141-153,
https://doi.org/10.2298/JSC190416062G . .

TY  - JOUR
AU  - Grbović, Filip
AU  - Gajić, Gordana
AU  - Branković, Snežana
AU  - Simić, Zoran
AU  - Ćirić, Andrija
AU  - Rakonjac, Ljubinko
AU  - Pavlović, Pavle
AU  - Topuzović, Marina
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800050G
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3353
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3354
AB  - The objective of this study was to determine the allelopathic potential of Robinia pseudoacacia L., Ailanthus altissima (Mill.) Swingle and Amorpha fruticosa L. that grow on the fly-ash deposits at the “Nikola Tesla – A” thermoelectric power plant in Obrenovac. The chemical characteristics of fly ash, such as pH, electrical conductivity (EC), content of carbon (C) and nitrogen (N), contents of available phosphorus (P2O5) and potassium (K2O), the contents of total and available Fe, Cu, Mn, Ni and Zn as well as of phenolic acids (3,5 dihydroxybenzoic acid (3,5-DHBA) and ferulic acid) and flavonoids (rutin and quercetin) were analyzed in control fly ash (bare zones without vegetation cover) and plant rhizospheric fly ash. In order to determine the allelopathic activity of phenolic compounds in fly ash, modified soil sandwich allelopathic biotests were performed, and Trifolium pratense L. (red clover) was used as the indicator species. A. fruticosa showed the highest allelopathic activity, followed by A. altissima whereas R. pseudoacacia showed the lowest allelopathic potential. Negative correlation was noted between radicle and hypocotyl growth inhibition of red clover and the pH of fly ash. Positive correlations were found between radicle growth inhibition and the content of C, P2O5, total concentrations of Cu, available concentrations of Mn and Ni, the contents of ferulic acid, 3,5-DHBA, and rutin. Our results indicate that A. fruticosa and A. altissima increased the content of phenolics in fly ash, which can act as allelochemicals leading to radicle growth inhibition of red clover in the pioneer plant community on fly-ash deposits. These woody species that colonized fly-ash deposits can initiate the beginning of pedogenetic processes altering the ecosystem processes at degraded sites
T2  - Archives of Biological Sciences
T1  - Allelopathic potential of selected woody species growing on fly-ash deposits
IS  - 1
VL  - 71
DO  - 10.2298/ABS180823050G
SP  - 83
EP  - 94
ER  - 

@article{
author = "Grbović, Filip and Gajić, Gordana and Branković, Snežana and Simić, Zoran and Ćirić, Andrija and Rakonjac, Ljubinko and Pavlović, Pavle and Topuzović, Marina",
year = "2019",
abstract = "The objective of this study was to determine the allelopathic potential of Robinia pseudoacacia L., Ailanthus altissima (Mill.) Swingle and Amorpha fruticosa L. that grow on the fly-ash deposits at the “Nikola Tesla – A” thermoelectric power plant in Obrenovac. The chemical characteristics of fly ash, such as pH, electrical conductivity (EC), content of carbon (C) and nitrogen (N), contents of available phosphorus (P2O5) and potassium (K2O), the contents of total and available Fe, Cu, Mn, Ni and Zn as well as of phenolic acids (3,5 dihydroxybenzoic acid (3,5-DHBA) and ferulic acid) and flavonoids (rutin and quercetin) were analyzed in control fly ash (bare zones without vegetation cover) and plant rhizospheric fly ash. In order to determine the allelopathic activity of phenolic compounds in fly ash, modified soil sandwich allelopathic biotests were performed, and Trifolium pratense L. (red clover) was used as the indicator species. A. fruticosa showed the highest allelopathic activity, followed by A. altissima whereas R. pseudoacacia showed the lowest allelopathic potential. Negative correlation was noted between radicle and hypocotyl growth inhibition of red clover and the pH of fly ash. Positive correlations were found between radicle growth inhibition and the content of C, P2O5, total concentrations of Cu, available concentrations of Mn and Ni, the contents of ferulic acid, 3,5-DHBA, and rutin. Our results indicate that A. fruticosa and A. altissima increased the content of phenolics in fly ash, which can act as allelochemicals leading to radicle growth inhibition of red clover in the pioneer plant community on fly-ash deposits. These woody species that colonized fly-ash deposits can initiate the beginning of pedogenetic processes altering the ecosystem processes at degraded sites",
journal = "Archives of Biological Sciences",
title = "Allelopathic potential of selected woody species growing on fly-ash deposits",
number = "1",
volume = "71",
doi = "10.2298/ABS180823050G",
pages = "83-94"
}

Grbović, F., Gajić, G., Branković, S., Simić, Z., Ćirić, A., Rakonjac, L., Pavlović, P.,& Topuzović, M.. (2019). Allelopathic potential of selected woody species growing on fly-ash deposits. in Archives of Biological Sciences, 71(1), 83-94.
https://doi.org/10.2298/ABS180823050G

Grbović F, Gajić G, Branković S, Simić Z, Ćirić A, Rakonjac L, Pavlović P, Topuzović M. Allelopathic potential of selected woody species growing on fly-ash deposits. in Archives of Biological Sciences. 2019;71(1):83-94.
doi:10.2298/ABS180823050G .

Grbović, Filip, Gajić, Gordana, Branković, Snežana, Simić, Zoran, Ćirić, Andrija, Rakonjac, Ljubinko, Pavlović, Pavle, Topuzović, Marina, "Allelopathic potential of selected woody species growing on fly-ash deposits" in Archives of Biological Sciences, 71, no. 1 (2019):83-94,
https://doi.org/10.2298/ABS180823050G . .

TY  - JOUR
AU  - Milutinović, Milena G.
AU  - Maksimović, Vuk M.
AU  - Cvetković, Danijela M.
AU  - Nikodijević, Danijela D.
AU  - Stanković, Milan S.
AU  - Pešić, Milica
AU  - Marković, Snežana D.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0378874119303010?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3406
AB  - ETHNOPHARMACOLOGICAL RELEVANCE Teucrum chamaedrys L. is one of the known medicinal plants, useful for treatment of various health problems, especially digestive. In this study, we investigated methanol, ethyl-acetate and acetone extracts of T. chamaedrys in respect to their anticancer properties in SW480 colorectal cancer cells. MATERIALS AND METHODS Cytotoxicity and proapoptotic potential were assessed by MTT cell viability assay and AO/EB double staining. Molecular mechanisms of induced apoptosis were determined by monitoring Fas receptor protein expression through immunofluorescence, Caspase 8 and 9 activity, as well as concentrations of O2.- spectrophotometrically. Additionally, mRNA expression of biotransformation enzymes (CYP1A1, CYP1B1, GSTP1) and membrane transporters (MRP1 and MRP2) involved in drug resistance were investigated by qPCR method. Qualitative analysis of individual phenolic compounds was performed by reversed phase HPLC-MS analysis. RESULTS Methanol extract shows the best cytotoxicity and selectivity compared to ethyl-acetate and acetone extracts, mainly causing apoptosis of SW480 cells, without affecting normal HaCaT keratinocytes. The increased expression of Fas receptor protein and caspase 8 activity indicate that the death receptor-mediated pathway plays a crucial role in the observed apoptosis. The increased caspase 9 activity and O2.- concentration suggest that mitochondria are also involved in the apoptosis. T. chamaedrys methanol extract inhibits mRNA expression of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 in SW480 cells. CONCLUSIONS Induction of apoptosis and inhibition of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 mRNA expression implies that T. chamaedrys can serve as a valuable source of bioactive compounds as dietary supplements or selective anticancer agents, with the ability to induce apoptosis and modulate drug resistance in colorectal cancer cells.
T2  - Journal of Ethnopharmacology
T1  - Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.
VL  - 240
DO  - 10.1016/j.jep.2019.111951
SP  - 111951
ER  - 

@article{
author = "Milutinović, Milena G. and Maksimović, Vuk M. and Cvetković, Danijela M. and Nikodijević, Danijela D. and Stanković, Milan S. and Pešić, Milica and Marković, Snežana D.",
year = "2019",
abstract = "ETHNOPHARMACOLOGICAL RELEVANCE Teucrum chamaedrys L. is one of the known medicinal plants, useful for treatment of various health problems, especially digestive. In this study, we investigated methanol, ethyl-acetate and acetone extracts of T. chamaedrys in respect to their anticancer properties in SW480 colorectal cancer cells. MATERIALS AND METHODS Cytotoxicity and proapoptotic potential were assessed by MTT cell viability assay and AO/EB double staining. Molecular mechanisms of induced apoptosis were determined by monitoring Fas receptor protein expression through immunofluorescence, Caspase 8 and 9 activity, as well as concentrations of O2.- spectrophotometrically. Additionally, mRNA expression of biotransformation enzymes (CYP1A1, CYP1B1, GSTP1) and membrane transporters (MRP1 and MRP2) involved in drug resistance were investigated by qPCR method. Qualitative analysis of individual phenolic compounds was performed by reversed phase HPLC-MS analysis. RESULTS Methanol extract shows the best cytotoxicity and selectivity compared to ethyl-acetate and acetone extracts, mainly causing apoptosis of SW480 cells, without affecting normal HaCaT keratinocytes. The increased expression of Fas receptor protein and caspase 8 activity indicate that the death receptor-mediated pathway plays a crucial role in the observed apoptosis. The increased caspase 9 activity and O2.- concentration suggest that mitochondria are also involved in the apoptosis. T. chamaedrys methanol extract inhibits mRNA expression of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 in SW480 cells. CONCLUSIONS Induction of apoptosis and inhibition of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 mRNA expression implies that T. chamaedrys can serve as a valuable source of bioactive compounds as dietary supplements or selective anticancer agents, with the ability to induce apoptosis and modulate drug resistance in colorectal cancer cells.",
journal = "Journal of Ethnopharmacology",
title = "Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.",
volume = "240",
doi = "10.1016/j.jep.2019.111951",
pages = "111951"
}

Milutinović, M. G., Maksimović, V. M., Cvetković, D. M., Nikodijević, D. D., Stanković, M. S., Pešić, M.,& Marković, S. D.. (2019). Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.. in Journal of Ethnopharmacology, 240, 111951.
https://doi.org/10.1016/j.jep.2019.111951

Milutinović MG, Maksimović VM, Cvetković DM, Nikodijević DD, Stanković MS, Pešić M, Marković SD. Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.. in Journal of Ethnopharmacology. 2019;240:111951.
doi:10.1016/j.jep.2019.111951 .

Milutinović, Milena G., Maksimović, Vuk M., Cvetković, Danijela M., Nikodijević, Danijela D., Stanković, Milan S., Pešić, Milica, Marković, Snežana D., "Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells." in Journal of Ethnopharmacology, 240 (2019):111951,
https://doi.org/10.1016/j.jep.2019.111951 . .

TY  - JOUR
AU  - Avdović, Edina H.
AU  - Stojković, Danijela L.J.
AU  - Jevtić, Verica V.
AU  - Kosić, Milica
AU  - Ristić, Biljana
AU  - Harhaji-Trajković, Ljubica
AU  - Vukić, Milena
AU  - Vuković, Nenad
AU  - Marković, Zoran S.
AU  - Potočňák, Ivan
AU  - Trifunović, Srećko R.
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0020169317301597
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2792
AB  - The new coumarine derivative, 3-(1-(3-hydroxypropylamino)ethylidene)chroman-2,4-dione, and corresponding palladium(II) complex have been synthesized and characterized by microanalysis, infrared, 1H and 13C NMR spectroscopy. The structure of the ligand, solved using a monocrystal X-ray structural analysis, consists of two crystallographic different pseudocentrosymmetrically related molecules of 3-(1-(3-hydroxypropylamino)ethylidene)chroman-2,4-dione, while the structure of the square-planar palladium(II) complex was proposed on the basis of DFT calculations. The palladium(II) complex decreased viability of U251 human glioma and B16 mouse melanoma cells in a dose and time dependent manner, while its ligand exhibited only moderate cytotoxicity.
T2  - Inorganica Chimica Acta
T1  - Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarin-derived ligand 3-(1-(3-hydroxypropylamino)ethylidene)chroman-2,4-dione. Crystal structure of the 3-(1-(3-hydroxypropylamino)ethylidene)-chroman-2,4-dione
VL  - 466
DO  - 10.1016/j.ica.2017.06.015
SP  - 188
EP  - 196
ER  - 

@article{
author = "Avdović, Edina H. and Stojković, Danijela L.J. and Jevtić, Verica V. and Kosić, Milica and Ristić, Biljana and Harhaji-Trajković, Ljubica and Vukić, Milena and Vuković, Nenad and Marković, Zoran S. and Potočňák, Ivan and Trifunović, Srećko R.",
year = "2017",
abstract = "The new coumarine derivative, 3-(1-(3-hydroxypropylamino)ethylidene)chroman-2,4-dione, and corresponding palladium(II) complex have been synthesized and characterized by microanalysis, infrared, 1H and 13C NMR spectroscopy. The structure of the ligand, solved using a monocrystal X-ray structural analysis, consists of two crystallographic different pseudocentrosymmetrically related molecules of 3-(1-(3-hydroxypropylamino)ethylidene)chroman-2,4-dione, while the structure of the square-planar palladium(II) complex was proposed on the basis of DFT calculations. The palladium(II) complex decreased viability of U251 human glioma and B16 mouse melanoma cells in a dose and time dependent manner, while its ligand exhibited only moderate cytotoxicity.",
journal = "Inorganica Chimica Acta",
title = "Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarin-derived ligand 3-(1-(3-hydroxypropylamino)ethylidene)chroman-2,4-dione. Crystal structure of the 3-(1-(3-hydroxypropylamino)ethylidene)-chroman-2,4-dione",
volume = "466",
doi = "10.1016/j.ica.2017.06.015",
pages = "188-196"
}

Avdović, E. H., Stojković, D. L.J., Jevtić, V. V., Kosić, M., Ristić, B., Harhaji-Trajković, L., Vukić, M., Vuković, N., Marković, Z. S., Potočňák, I.,& Trifunović, S. R.. (2017). Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarin-derived ligand 3-(1-(3-hydroxypropylamino)ethylidene)chroman-2,4-dione. Crystal structure of the 3-(1-(3-hydroxypropylamino)ethylidene)-chroman-2,4-dione. in Inorganica Chimica Acta, 466, 188-196.
https://doi.org/10.1016/j.ica.2017.06.015

Avdović EH, Stojković DL, Jevtić VV, Kosić M, Ristić B, Harhaji-Trajković L, Vukić M, Vuković N, Marković ZS, Potočňák I, Trifunović SR. Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarin-derived ligand 3-(1-(3-hydroxypropylamino)ethylidene)chroman-2,4-dione. Crystal structure of the 3-(1-(3-hydroxypropylamino)ethylidene)-chroman-2,4-dione. in Inorganica Chimica Acta. 2017;466:188-196.
doi:10.1016/j.ica.2017.06.015 .

Avdović, Edina H., Stojković, Danijela L.J., Jevtić, Verica V., Kosić, Milica, Ristić, Biljana, Harhaji-Trajković, Ljubica, Vukić, Milena, Vuković, Nenad, Marković, Zoran S., Potočňák, Ivan, Trifunović, Srećko R., "Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarin-derived ligand 3-(1-(3-hydroxypropylamino)ethylidene)chroman-2,4-dione. Crystal structure of the 3-(1-(3-hydroxypropylamino)ethylidene)-chroman-2,4-dione" in Inorganica Chimica Acta, 466 (2017):188-196,
https://doi.org/10.1016/j.ica.2017.06.015 . .

TY  - JOUR
AU  - Katanić, Jelena
AU  - Matić, Sanja
AU  - Pferschy-Wenzig, Eva-Maria
AU  - Kretschmer, Nadine
AU  - Boroja, Tatjana
AU  - Mihailović, Vladimir
AU  - Stanković, Vesna
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Bauer, Rudolf
PY  - 2017
UR  - https://www.sciencedirect.com/science/article/pii/S0278691516304343?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3178
AB  - Filipendula ulmaria, known as meadowsweet, is a perennial herb found in wild and cultivated habitats in Europe and Asia. Usage of F. ulmaria in traditional medicine is based on diuretic, astringent, antirheumatic, and anti-inflammatory properties of this plant. Exposure to cisplatin at a dose of 7.5 mg/kg caused significant increase in serum parameters of liver and kidneys function and tissue oxidative stress markers along with some histopathological changes in liver and kidney tissues of experimental rats, as well as high level of genotoxicity. Administration of F. ulmaria extracts in three different concentrations (100, 200, and 400 mg/kg/day) for 10 days resulted in a reduction of oxidative stress in tissues and decrease of serum parameters. Moreover, tested extracts attenuated the genotoxicity of cisplatin in reverse dose-dependent manner. F. ulmaria extracts had no in vitro cytotoxic activity at all applied concentrations (IC50 > 50 μg/mL). Tested extracts, rich in polyphenolic compounds, attenuate cisplatin-induced liver and kidney oxidative stress, reduce tissue damage, and enhance the antioxidative status of experimental animals during cisplatin application. Therefore, F. ulmaria extracts may be used as supportive agent for the prevention and amelioration of cisplatin side effects.
T2  - Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association
T1  - Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.
VL  - 99
DO  - 10.1016/j.fct.2016.11.018
SP  - 86
EP  - 102
ER  - 

@article{
author = "Katanić, Jelena and Matić, Sanja and Pferschy-Wenzig, Eva-Maria and Kretschmer, Nadine and Boroja, Tatjana and Mihailović, Vladimir and Stanković, Vesna and Stanković, Nevena and Mladenović, Milan and Stanić, Snežana and Mihailović, Mirjana and Bauer, Rudolf",
year = "2017",
abstract = "Filipendula ulmaria, known as meadowsweet, is a perennial herb found in wild and cultivated habitats in Europe and Asia. Usage of F. ulmaria in traditional medicine is based on diuretic, astringent, antirheumatic, and anti-inflammatory properties of this plant. Exposure to cisplatin at a dose of 7.5 mg/kg caused significant increase in serum parameters of liver and kidneys function and tissue oxidative stress markers along with some histopathological changes in liver and kidney tissues of experimental rats, as well as high level of genotoxicity. Administration of F. ulmaria extracts in three different concentrations (100, 200, and 400 mg/kg/day) for 10 days resulted in a reduction of oxidative stress in tissues and decrease of serum parameters. Moreover, tested extracts attenuated the genotoxicity of cisplatin in reverse dose-dependent manner. F. ulmaria extracts had no in vitro cytotoxic activity at all applied concentrations (IC50 > 50 μg/mL). Tested extracts, rich in polyphenolic compounds, attenuate cisplatin-induced liver and kidney oxidative stress, reduce tissue damage, and enhance the antioxidative status of experimental animals during cisplatin application. Therefore, F. ulmaria extracts may be used as supportive agent for the prevention and amelioration of cisplatin side effects.",
journal = "Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association",
title = "Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.",
volume = "99",
doi = "10.1016/j.fct.2016.11.018",
pages = "86-102"
}

Katanić, J., Matić, S., Pferschy-Wenzig, E., Kretschmer, N., Boroja, T., Mihailović, V., Stanković, V., Stanković, N., Mladenović, M., Stanić, S., Mihailović, M.,& Bauer, R.. (2017). Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.. in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association, 99, 86-102.
https://doi.org/10.1016/j.fct.2016.11.018

Katanić J, Matić S, Pferschy-Wenzig E, Kretschmer N, Boroja T, Mihailović V, Stanković V, Stanković N, Mladenović M, Stanić S, Mihailović M, Bauer R. Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.. in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association. 2017;99:86-102.
doi:10.1016/j.fct.2016.11.018 .

Katanić, Jelena, Matić, Sanja, Pferschy-Wenzig, Eva-Maria, Kretschmer, Nadine, Boroja, Tatjana, Mihailović, Vladimir, Stanković, Vesna, Stanković, Nevena, Mladenović, Milan, Stanić, Snežana, Mihailović, Mirjana, Bauer, Rudolf, "Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis." in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association, 99 (2017):86-102,
https://doi.org/10.1016/j.fct.2016.11.018 . .

TY  - JOUR
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
PY  - 2016
UR  - http://www.sciencedirect.com/science/article/pii/S1319562X15001138
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1319562X15001138
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3177
AB  - The Anacardiaceae Lindl. family comprises of many species which are used in nutrition and in traditional folk medicine for the treatment of several human diseases. Cotinus coggygria Scop. commonly known as “smoke tree”, is an commercial ornamental plant with high medicinal usages, belongs to the family Anacardiaceae. The present review provides a comprehensive report of empirical investigations on important pharmacological activities and phytochemical screening of essential oils and extracts. Relevant information was collected from scientific journals, books, and reports via library and electronic search using Medline, Pubmed, Google Scholar, ScienceDirect, Web of Science, and Scopus. The plant has been extensively investigated in a broad range of studies to provide scientific evidence for folklore claims or to find new therapeutic uses. Numerous activities namely antioxidative, antibacterial, antifungal, antiviral, anticancer, antigenotoxic, hepatoprotective and anti-inflammatory have been demonstrated for all parts of these plants by in vivo and in vitro studies. Essential oils and extracts showed various pharmacological and biological properties which make them an effective remedy for various kinds of illnesses. Considering data from the literature, it could be demonstrated that C. coggygria possesses diverse bioactive properties and immense utilization in medicine, health care, cosmetics and as health supplements.
T2  - Saudi Journal of Biological Sciences
T1  - Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential
IS  - 4
VL  - 23
DO  - 10.1016/j.sjbs.2015.05.012
SP  - 452
EP  - 461
ER  - 

@article{
author = "Matić, Sanja and Stanić, Snežana and Mihailović, Mirjana and Bogojević, Desanka",
year = "2016",
abstract = "The Anacardiaceae Lindl. family comprises of many species which are used in nutrition and in traditional folk medicine for the treatment of several human diseases. Cotinus coggygria Scop. commonly known as “smoke tree”, is an commercial ornamental plant with high medicinal usages, belongs to the family Anacardiaceae. The present review provides a comprehensive report of empirical investigations on important pharmacological activities and phytochemical screening of essential oils and extracts. Relevant information was collected from scientific journals, books, and reports via library and electronic search using Medline, Pubmed, Google Scholar, ScienceDirect, Web of Science, and Scopus. The plant has been extensively investigated in a broad range of studies to provide scientific evidence for folklore claims or to find new therapeutic uses. Numerous activities namely antioxidative, antibacterial, antifungal, antiviral, anticancer, antigenotoxic, hepatoprotective and anti-inflammatory have been demonstrated for all parts of these plants by in vivo and in vitro studies. Essential oils and extracts showed various pharmacological and biological properties which make them an effective remedy for various kinds of illnesses. Considering data from the literature, it could be demonstrated that C. coggygria possesses diverse bioactive properties and immense utilization in medicine, health care, cosmetics and as health supplements.",
journal = "Saudi Journal of Biological Sciences",
title = "Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential",
number = "4",
volume = "23",
doi = "10.1016/j.sjbs.2015.05.012",
pages = "452-461"
}

Matić, S., Stanić, S., Mihailović, M.,& Bogojević, D.. (2016). Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential. in Saudi Journal of Biological Sciences, 23(4), 452-461.
https://doi.org/10.1016/j.sjbs.2015.05.012

Matić S, Stanić S, Mihailović M, Bogojević D. Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential. in Saudi Journal of Biological Sciences. 2016;23(4):452-461.
doi:10.1016/j.sjbs.2015.05.012 .

Matić, Sanja, Stanić, Snežana, Mihailović, Mirjana, Bogojević, Desanka, "Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential" in Saudi Journal of Biological Sciences, 23, no. 4 (2016):452-461,
https://doi.org/10.1016/j.sjbs.2015.05.012 . .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Boroja, Tatjana
AU  - Vuković, Nenad
PY  - 2015
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84943457635&partnerID=tZOtx3y1
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0006295215005511
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3175
AB  - Eight chroman-2,4-diones, namely 2a-h, previously investigated as anticoagulants, of which 2a and 2f as the most active, were evaluated as in vivo genotoxic agents in Wistar rat livers and kidneys using the comet assay. Compounds 2a, 2b, and 2f without genotoxic activity were applied prior to ethyl methanesulfonate (EMS) and diminished EMS-induced DNA damage according to the total score and percentage of reduction. EMS produce harmful O(6)-ethylguanine lesion which is incorporated in aberrant genotoxic GT and TG pairing after ATP-dependent DNA strand breaks have been catalyzed by rat Topoisomerase IIα (rTopIIα, EC 5.99.1.3). Therefore, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on the enzyme level using molecular docking and molecular dynamics simulations insamuch as it had been determined that compounds do not intercalate DNA but instead inhibit the ATPase activity. Calculations predicted that compounds inhibit ATP hydrolysis before the DNA-EMS cleavage is being catalyzed by rTopIIα, prevent EMS mutagenic and carcinogenic effects, and beside anticoagulant activity can even be applied in the cancer treatment to control the rate of anticancer alkylation drugs.
PB  - Elsevier
T2  - Biochemical Pharmacology
T1  - Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach
IS  - 1
VL  - 98
DO  - 10.1016/j.bcp.2015.08.106
SP  - 243
EP  - 266
ER  - 

@article{
author = "Mladenović, Milan and Stanković, Nevena and Matić, Sanja and Stanić, Snežana and Mihailović, Mirjana and Mihailović, Vladimir and Katanić, Jelena and Boroja, Tatjana and Vuković, Nenad",
year = "2015",
abstract = "Eight chroman-2,4-diones, namely 2a-h, previously investigated as anticoagulants, of which 2a and 2f as the most active, were evaluated as in vivo genotoxic agents in Wistar rat livers and kidneys using the comet assay. Compounds 2a, 2b, and 2f without genotoxic activity were applied prior to ethyl methanesulfonate (EMS) and diminished EMS-induced DNA damage according to the total score and percentage of reduction. EMS produce harmful O(6)-ethylguanine lesion which is incorporated in aberrant genotoxic GT and TG pairing after ATP-dependent DNA strand breaks have been catalyzed by rat Topoisomerase IIα (rTopIIα, EC 5.99.1.3). Therefore, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on the enzyme level using molecular docking and molecular dynamics simulations insamuch as it had been determined that compounds do not intercalate DNA but instead inhibit the ATPase activity. Calculations predicted that compounds inhibit ATP hydrolysis before the DNA-EMS cleavage is being catalyzed by rTopIIα, prevent EMS mutagenic and carcinogenic effects, and beside anticoagulant activity can even be applied in the cancer treatment to control the rate of anticancer alkylation drugs.",
publisher = "Elsevier",
journal = "Biochemical Pharmacology",
title = "Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach",
number = "1",
volume = "98",
doi = "10.1016/j.bcp.2015.08.106",
pages = "243-266"
}

Mladenović, M., Stanković, N., Matić, S., Stanić, S., Mihailović, M., Mihailović, V., Katanić, J., Boroja, T.,& Vuković, N.. (2015). Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach. in Biochemical Pharmacology
Elsevier., 98(1), 243-266.
https://doi.org/10.1016/j.bcp.2015.08.106

Mladenović M, Stanković N, Matić S, Stanić S, Mihailović M, Mihailović V, Katanić J, Boroja T, Vuković N. Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach. in Biochemical Pharmacology. 2015;98(1):243-266.
doi:10.1016/j.bcp.2015.08.106 .

Mladenović, Milan, Stanković, Nevena, Matić, Sanja, Stanić, Snežana, Mihailović, Mirjana, Mihailović, Vladimir, Katanić, Jelena, Boroja, Tatjana, Vuković, Nenad, "Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach" in Biochemical Pharmacology, 98, no. 1 (2015):243-266,
https://doi.org/10.1016/j.bcp.2015.08.106 . .

TY  - JOUR
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Stanković, Vesna
AU  - Mihailović, Mirjana
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Boroja, Tatjana
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2004
AB  - Two chroman-2,4-dione derivatives, namely 2a and 2f, were tested as in
   vivo anticoagulants by seven days of continuous per os application to
   adult male Wistar rats in a concentration of 20 mg/kg of body weight.
   Derivatives were selected from a group of six previously
   intraperitoneally applied compounds on the basis of presenting
   remarkable activity in a concentration of 2 mg/kg of body weight. The
   derivatives 2a and 2f are VKORC1 inhibitors, and comparison of the
   absorption spectra, association, and dissociation constants suggested
   that the compounds will be bound to serum albumin in the same manner as
   warfarin is, leading to transfer towards the molecular target VKORC1.
   After oral administration, the compounds proved to be anticoagulants
   comparable with warfarin, inasmuch as the measured prothrombin times for
   2a and 2f were 56.63 and 60.08 s, respectively. The INR values of 2a and
   2f ranged from 2.6 to 2.8, recommending them as useful therapeutics in
   the treatment of patients suffering from thromboembolic events and
   atrial fibrillation. The high percentage of binding and high binding
   affinity of 2a and 2f towards serum albumin reduced the risk of induced
   internal bleeding. Several kinds of toxicity studies were performed to
   investigate whether or not 2a and 2f can cause pathological changes in
   the liver, kidneys, and DNA. The catalytic activity of serum enzymes,
   concentration and catalytic activity of liver and kidney oxidative
   stress markers and enzymes, respectively, as well as the observed
   hepatic and renal morphological changes indicated that the compounds in
   relation to warfarin induced irrelevant hepatic toxicity, no increment
   of necrosis, and inconsiderable oxidative damage in the liver and
   kidneys. Estimation of DNA damage using the comet assay confirmed that
   2a and 2f caused no clinically significant genotoxicity. The higher
   activity and lower toxicity of 2f recommended this compound as a better
   drug candidate than 2a. (C) 2014 Elsevier Ireland Ltd. All rights
   reserved.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants
VL  - 227
DO  - 10.1016/j.cbi.2014.12.005
SP  - 18
EP  - 31
ER  - 

@article{
author = "Stanković, Nevena and Mladenović, Milan and Matić, Sanja and Stanić, Snežana and Stanković, Vesna and Mihailović, Mirjana and Mihailović, Vladimir and Katanić, Jelena and Boroja, Tatjana and Vuković, Nenad and Sukdolak, Slobodan",
year = "2015",
abstract = "Two chroman-2,4-dione derivatives, namely 2a and 2f, were tested as in
   vivo anticoagulants by seven days of continuous per os application to
   adult male Wistar rats in a concentration of 20 mg/kg of body weight.
   Derivatives were selected from a group of six previously
   intraperitoneally applied compounds on the basis of presenting
   remarkable activity in a concentration of 2 mg/kg of body weight. The
   derivatives 2a and 2f are VKORC1 inhibitors, and comparison of the
   absorption spectra, association, and dissociation constants suggested
   that the compounds will be bound to serum albumin in the same manner as
   warfarin is, leading to transfer towards the molecular target VKORC1.
   After oral administration, the compounds proved to be anticoagulants
   comparable with warfarin, inasmuch as the measured prothrombin times for
   2a and 2f were 56.63 and 60.08 s, respectively. The INR values of 2a and
   2f ranged from 2.6 to 2.8, recommending them as useful therapeutics in
   the treatment of patients suffering from thromboembolic events and
   atrial fibrillation. The high percentage of binding and high binding
   affinity of 2a and 2f towards serum albumin reduced the risk of induced
   internal bleeding. Several kinds of toxicity studies were performed to
   investigate whether or not 2a and 2f can cause pathological changes in
   the liver, kidneys, and DNA. The catalytic activity of serum enzymes,
   concentration and catalytic activity of liver and kidney oxidative
   stress markers and enzymes, respectively, as well as the observed
   hepatic and renal morphological changes indicated that the compounds in
   relation to warfarin induced irrelevant hepatic toxicity, no increment
   of necrosis, and inconsiderable oxidative damage in the liver and
   kidneys. Estimation of DNA damage using the comet assay confirmed that
   2a and 2f caused no clinically significant genotoxicity. The higher
   activity and lower toxicity of 2f recommended this compound as a better
   drug candidate than 2a. (C) 2014 Elsevier Ireland Ltd. All rights
   reserved.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants",
volume = "227",
doi = "10.1016/j.cbi.2014.12.005",
pages = "18-31"
}

Stanković, N., Mladenović, M., Matić, S., Stanić, S., Stanković, V., Mihailović, M., Mihailović, V., Katanić, J., Boroja, T., Vuković, N.,& Sukdolak, S.. (2015). Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants. in Chemico-Biological Interactions
Elsevier., 227, 18-31.
https://doi.org/10.1016/j.cbi.2014.12.005

Stanković N, Mladenović M, Matić S, Stanić S, Stanković V, Mihailović M, Mihailović V, Katanić J, Boroja T, Vuković N, Sukdolak S. Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants. in Chemico-Biological Interactions. 2015;227:18-31.
doi:10.1016/j.cbi.2014.12.005 .

Stanković, Nevena, Mladenović, Milan, Matić, Sanja, Stanić, Snežana, Stanković, Vesna, Mihailović, Mirjana, Mihailović, Vladimir, Katanić, Jelena, Boroja, Tatjana, Vuković, Nenad, Sukdolak, Slobodan, "Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants" in Chemico-Biological Interactions, 227 (2015):18-31,
https://doi.org/10.1016/j.cbi.2014.12.005 . .

TY  - JOUR
AU  - Katanić, Jelena
AU  - Mihailović, Vladimir
AU  - Matić, Sanja
AU  - Stanković, Vesna
AU  - Stanković, Nevena
AU  - Boroja, Tatjana
AU  - Mladenović, Milan
AU  - Stanić, Snezana
AU  - Kreft, Samo
AU  - Mihailović, Mirjana
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2352
UR  - http://www.sciencedirect.com/science/article/pii/S175646461500362X
AB  - The effects of the methanolic extracts of Filipendula hexapetala Gilib.
   aerial parts (FHA) and roots (FHR) against cisplatin induced kidney and
   liver injuries in rats were investigated as well as determination of
   genotoxicity and antigenotoxicity of the extracts. Treatment with FHA
   and FHR significantly decreased levels of urea, uric acid, serum
   transaminases, alkaline phosphatase and gamma-glutamyl transferase, and
   increased the content of total protein. In addition, treatment with the
   extracts significantly attenuated the cisplatin-induced oxidative stress
   in kidney and liver tissues by increasing catalase and superoxide
   dismutase activities and the content of reduced glutathione and
   decreasing the content of thiobarbituric acid reactive substances
   (TSARS). The histopathological studies confirmed the protective effects
   of the extracts against cisplatin-induced kidney and liver injuries. The
   extracts ameliorated cisplatin-induced genotoxicity. These results
   suggest that F. hexapetala extracts are effective nephro- and
   hepatoprotective agents, with potential to reduce oxidative stress and
   ameliorate cisplatin-induced nephro- and hepatotoxicity.
T2  - Journal of Functional Foods
T1  - The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin
IS  - Part A
VL  - 18
DO  - 10.1016/j.jff.2015.07.004
SP  - 198
EP  - 212
ER  - 

@article{
author = "Katanić, Jelena and Mihailović, Vladimir and Matić, Sanja and Stanković, Vesna and Stanković, Nevena and Boroja, Tatjana and Mladenović, Milan and Stanić, Snezana and Kreft, Samo and Mihailović, Mirjana",
year = "2015",
abstract = "The effects of the methanolic extracts of Filipendula hexapetala Gilib.
   aerial parts (FHA) and roots (FHR) against cisplatin induced kidney and
   liver injuries in rats were investigated as well as determination of
   genotoxicity and antigenotoxicity of the extracts. Treatment with FHA
   and FHR significantly decreased levels of urea, uric acid, serum
   transaminases, alkaline phosphatase and gamma-glutamyl transferase, and
   increased the content of total protein. In addition, treatment with the
   extracts significantly attenuated the cisplatin-induced oxidative stress
   in kidney and liver tissues by increasing catalase and superoxide
   dismutase activities and the content of reduced glutathione and
   decreasing the content of thiobarbituric acid reactive substances
   (TSARS). The histopathological studies confirmed the protective effects
   of the extracts against cisplatin-induced kidney and liver injuries. The
   extracts ameliorated cisplatin-induced genotoxicity. These results
   suggest that F. hexapetala extracts are effective nephro- and
   hepatoprotective agents, with potential to reduce oxidative stress and
   ameliorate cisplatin-induced nephro- and hepatotoxicity.",
journal = "Journal of Functional Foods",
title = "The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin",
number = "Part A",
volume = "18",
doi = "10.1016/j.jff.2015.07.004",
pages = "198-212"
}

Katanić, J., Mihailović, V., Matić, S., Stanković, V., Stanković, N., Boroja, T., Mladenović, M., Stanić, S., Kreft, S.,& Mihailović, M.. (2015). The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin. in Journal of Functional Foods, 18(Part A), 198-212.
https://doi.org/10.1016/j.jff.2015.07.004

Katanić J, Mihailović V, Matić S, Stanković V, Stanković N, Boroja T, Mladenović M, Stanić S, Kreft S, Mihailović M. The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin. in Journal of Functional Foods. 2015;18(Part A):198-212.
doi:10.1016/j.jff.2015.07.004 .

Katanić, Jelena, Mihailović, Vladimir, Matić, Sanja, Stanković, Vesna, Stanković, Nevena, Boroja, Tatjana, Mladenović, Milan, Stanić, Snezana, Kreft, Samo, Mihailović, Mirjana, "The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin" in Journal of Functional Foods, 18, no. Part A (2015):198-212,
https://doi.org/10.1016/j.jff.2015.07.004 . .

TY  - JOUR
AU  - Marković, Snežana D.
AU  - Đorđević, Nataša Z.
AU  - Ćurčić, Milena G.
AU  - Štajn, Andraš Š.
AU  - Spasić, Mihajlo
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2151
AB  - The effects of nitroglycerin (glyceryl trinitrate - GTN) are mediated by
   liberated nitric oxide (NO) and formed reactive nitrogen species, which
   induces oxidative stress during biotransformation in red blood cells
   (RBCs). The aim of this study was to evaluate effects of GTN on
   antioxidative defense system (AOS) in rat erythrocytes (without) and
   reticulocytes (with functional mitochondria). Rat erythrocyte and
   reticulocyte-rich RBC suspensions were aerobically incubated (2 h, 37
   degrees C) without (control) or in the presence of different
   concentrations of GTN (0.1-1.5 mM). After incubation, concentrations of
   non-enzymatic components of AOS, activities of antioxidative enzymes and
   oxidative pentose phosphate (OPP) pathway activity were followed in RBC
   suspensions. In rat reticulocytes, GTN decreased the activity of
   mitochondrial MnSOD and increased the activity of CuZnSOD. In rat RBCs,
   GTN induced increase of Vit E concentration (at high doses), but
   decreased glutathione content and activities of all
   glutathione-dependent antioxidative enzymes; the OPP pathway activity
   significantly increased. GTN biotransformation and induction of
   oxidative stress were followed by general disbalance of antioxidative
   capacities in both kinds of RBCs. We suggest that oxidative stress,
   MnSOD inhibition and depletion of glutathione pool in response to GTN
   treatment lead to decreased bioavailability of NO after GTN
   biotransformation in rat reticulocytes.
T2  - General Physiology and Biophysics
T1  - Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes
IS  - 4
VL  - 33
DO  - 10.4149/gpb_2014018
SP  - 393
EP  - 401
ER  - 

@article{
author = "Marković, Snežana D. and Đorđević, Nataša Z. and Ćurčić, Milena G. and Štajn, Andraš Š. and Spasić, Mihajlo",
year = "2014",
abstract = "The effects of nitroglycerin (glyceryl trinitrate - GTN) are mediated by
   liberated nitric oxide (NO) and formed reactive nitrogen species, which
   induces oxidative stress during biotransformation in red blood cells
   (RBCs). The aim of this study was to evaluate effects of GTN on
   antioxidative defense system (AOS) in rat erythrocytes (without) and
   reticulocytes (with functional mitochondria). Rat erythrocyte and
   reticulocyte-rich RBC suspensions were aerobically incubated (2 h, 37
   degrees C) without (control) or in the presence of different
   concentrations of GTN (0.1-1.5 mM). After incubation, concentrations of
   non-enzymatic components of AOS, activities of antioxidative enzymes and
   oxidative pentose phosphate (OPP) pathway activity were followed in RBC
   suspensions. In rat reticulocytes, GTN decreased the activity of
   mitochondrial MnSOD and increased the activity of CuZnSOD. In rat RBCs,
   GTN induced increase of Vit E concentration (at high doses), but
   decreased glutathione content and activities of all
   glutathione-dependent antioxidative enzymes; the OPP pathway activity
   significantly increased. GTN biotransformation and induction of
   oxidative stress were followed by general disbalance of antioxidative
   capacities in both kinds of RBCs. We suggest that oxidative stress,
   MnSOD inhibition and depletion of glutathione pool in response to GTN
   treatment lead to decreased bioavailability of NO after GTN
   biotransformation in rat reticulocytes.",
journal = "General Physiology and Biophysics",
title = "Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes",
number = "4",
volume = "33",
doi = "10.4149/gpb_2014018",
pages = "393-401"
}

Marković, S. D., Đorđević, N. Z., Ćurčić, M. G., Štajn, A. Š.,& Spasić, M.. (2014). Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes. in General Physiology and Biophysics, 33(4), 393-401.
https://doi.org/10.4149/gpb_2014018

Marković SD, Đorđević NZ, Ćurčić MG, Štajn AŠ, Spasić M. Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes. in General Physiology and Biophysics. 2014;33(4):393-401.
doi:10.4149/gpb_2014018 .

Marković, Snežana D., Đorđević, Nataša Z., Ćurčić, Milena G., Štajn, Andraš Š., Spasić, Mihajlo, "Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes" in General Physiology and Biophysics, 33, no. 4 (2014):393-401,
https://doi.org/10.4149/gpb_2014018 . .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
AU  - Matić, Sanja
AU  - Nićiforović, Neda
AU  - Mihailović, Vladimir
AU  - Mašković, Pavle
AU  - Vrvić, Miroslav M.
AU  - Solujić, Slavica
PY  - 2012
UR  - https://www.sciencedirect.com/science/article/pii/S0223523412002887?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3201
AB  - The objective of this study was to investigate in vitro and in vivo anticoagulant activity of sixteen 4-hydroxycoumarin derivatives bearing polar C-3 scaffolds. The activity was evaluated by measuring prothrombin time. Enhanced anticoagulant activity in vitro was observed for all tested compounds. Upon successive administration of 0.5 mg/kg of body weight to adult Wistar rats, over a period of five days, four derivatives (2b, 4c, 5c and 9c) presented anticoagulant activity in vivo. The most active compound was 2b, with PT = 30.0 s. Low or non-toxic effects in vivo were determined based on the catalytic activity of liver enzymes and the concentration of bilirubin, iron and proteins. Metabolic pathways of the most active compounds in vivo were determined after GC/MS analysis of collected rat urine samples. The excretion occurs by glucuronidation of 7-hydroxy forms of tested derivatives. In vivo results were described using PLS-based CoMFA and CoMSIA 3D-QSAR studies, which showed CoMFA-SE (q2 = 0.738) and CoMSIA-SEA (q2 = 0.763) to be the statistically most relevant models. Furthermore, molecular docking and DFT mechanistic studies performed on the rat VKORC1 homology model revealed interactions between the 4-OH coumarin group in the form of phenolic anion and the Cys135 catalytic site in the transition state.
T2  - European Journal of Medicinal Chemistry
T1  - Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants
VL  - 54
DO  - 10.1016/J.EJMECH.2012.04.036
SP  - 144
EP  - 158
ER  - 

@article{
author = "Mladenović, Milan and Mihailović, Mirjana and Bogojević, Desanka and Vuković, Nenad and Sukdolak, Slobodan and Matić, Sanja and Nićiforović, Neda and Mihailović, Vladimir and Mašković, Pavle and Vrvić, Miroslav M. and Solujić, Slavica",
year = "2012",
abstract = "The objective of this study was to investigate in vitro and in vivo anticoagulant activity of sixteen 4-hydroxycoumarin derivatives bearing polar C-3 scaffolds. The activity was evaluated by measuring prothrombin time. Enhanced anticoagulant activity in vitro was observed for all tested compounds. Upon successive administration of 0.5 mg/kg of body weight to adult Wistar rats, over a period of five days, four derivatives (2b, 4c, 5c and 9c) presented anticoagulant activity in vivo. The most active compound was 2b, with PT = 30.0 s. Low or non-toxic effects in vivo were determined based on the catalytic activity of liver enzymes and the concentration of bilirubin, iron and proteins. Metabolic pathways of the most active compounds in vivo were determined after GC/MS analysis of collected rat urine samples. The excretion occurs by glucuronidation of 7-hydroxy forms of tested derivatives. In vivo results were described using PLS-based CoMFA and CoMSIA 3D-QSAR studies, which showed CoMFA-SE (q2 = 0.738) and CoMSIA-SEA (q2 = 0.763) to be the statistically most relevant models. Furthermore, molecular docking and DFT mechanistic studies performed on the rat VKORC1 homology model revealed interactions between the 4-OH coumarin group in the form of phenolic anion and the Cys135 catalytic site in the transition state.",
journal = "European Journal of Medicinal Chemistry",
title = "Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants",
volume = "54",
doi = "10.1016/J.EJMECH.2012.04.036",
pages = "144-158"
}

Mladenović, M., Mihailović, M., Bogojević, D., Vuković, N., Sukdolak, S., Matić, S., Nićiforović, N., Mihailović, V., Mašković, P., Vrvić, M. M.,& Solujić, S.. (2012). Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants. in European Journal of Medicinal Chemistry, 54, 144-158.
https://doi.org/10.1016/J.EJMECH.2012.04.036

Mladenović M, Mihailović M, Bogojević D, Vuković N, Sukdolak S, Matić S, Nićiforović N, Mihailović V, Mašković P, Vrvić MM, Solujić S. Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants. in European Journal of Medicinal Chemistry. 2012;54:144-158.
doi:10.1016/J.EJMECH.2012.04.036 .

Mladenović, Milan, Mihailović, Mirjana, Bogojević, Desanka, Vuković, Nenad, Sukdolak, Slobodan, Matić, Sanja, Nićiforović, Neda, Mihailović, Vladimir, Mašković, Pavle, Vrvić, Miroslav M., Solujić, Slavica, "Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants" in European Journal of Medicinal Chemistry, 54 (2012):144-158,
https://doi.org/10.1016/J.EJMECH.2012.04.036 . .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
AU  - Matić, Sanja
AU  - Nićiforović, Neda
AU  - Mihailović, Vladimir
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
AU  - Solujić, Slavica
PY  - 2011
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-79957795403&partnerID=tZOtx3y1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3207
AB  - The series of fifteen synthesized 4-hydroxycoumarin derivatives was subjected to antioxidant activity evaluation in vitro, through total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl radical, lipid peroxide scavenging and chelating activity. The highest activity was detected during the radicals scavenging, with 2b, 6b, 2c, and 4c noticed as the most active. The antioxidant activity was further quantified by the quantitative structure-activity relationships (QSAR) studies. For this purpose, the structures were optimized using Paramethric Method 6 (PM6) semi-empirical and Density Functional Theory (DFT) B3LYP methods. Bond dissociation enthalpies of coumarin 4-OH, Natural Bond Orbital (NBO) gained hybridization of the oxygen, acidity of the hydrogen atom and various molecular descriptors obtained, were correlated with biological activity, after which we designed 20 new antioxidant structures, using the most favorable structural motifs, with much improved predicted activity in vitro.
T2  - International Journal of Molecular Sciences
T1  - In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.
IS  - 5
VL  - 12
DO  - 10.3390/ijms12052822
SP  - 2822
EP  - 2841
ER  - 

@article{
author = "Mladenović, Milan and Mihailović, Mirjana and Bogojević, Desanka and Matić, Sanja and Nićiforović, Neda and Mihailović, Vladimir and Vuković, Nenad and Sukdolak, Slobodan and Solujić, Slavica",
year = "2011",
abstract = "The series of fifteen synthesized 4-hydroxycoumarin derivatives was subjected to antioxidant activity evaluation in vitro, through total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl radical, lipid peroxide scavenging and chelating activity. The highest activity was detected during the radicals scavenging, with 2b, 6b, 2c, and 4c noticed as the most active. The antioxidant activity was further quantified by the quantitative structure-activity relationships (QSAR) studies. For this purpose, the structures were optimized using Paramethric Method 6 (PM6) semi-empirical and Density Functional Theory (DFT) B3LYP methods. Bond dissociation enthalpies of coumarin 4-OH, Natural Bond Orbital (NBO) gained hybridization of the oxygen, acidity of the hydrogen atom and various molecular descriptors obtained, were correlated with biological activity, after which we designed 20 new antioxidant structures, using the most favorable structural motifs, with much improved predicted activity in vitro.",
journal = "International Journal of Molecular Sciences",
title = "In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.",
number = "5",
volume = "12",
doi = "10.3390/ijms12052822",
pages = "2822-2841"
}

Mladenović, M., Mihailović, M., Bogojević, D., Matić, S., Nićiforović, N., Mihailović, V., Vuković, N., Sukdolak, S.,& Solujić, S.. (2011). In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.. in International Journal of Molecular Sciences, 12(5), 2822-2841.
https://doi.org/10.3390/ijms12052822

Mladenović M, Mihailović M, Bogojević D, Matić S, Nićiforović N, Mihailović V, Vuković N, Sukdolak S, Solujić S. In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.. in International Journal of Molecular Sciences. 2011;12(5):2822-2841.
doi:10.3390/ijms12052822 .

Mladenović, Milan, Mihailović, Mirjana, Bogojević, Desanka, Matić, Sanja, Nićiforović, Neda, Mihailović, Vladimir, Vuković, Nenad, Sukdolak, Slobodan, Solujić, Slavica, "In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies." in International Journal of Molecular Sciences, 12, no. 5 (2011):2822-2841,
https://doi.org/10.3390/ijms12052822 . .