TY  - JOUR
AU  - Filipović, Biljana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Dragićević, Milan
AU  - Božunović, Jelena
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Bohanec, Borut
AU  - Milutinović, Milica
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5254
AB  - Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.
PB  - Amsterdam : Elsevier
T2  - Industrial Crops and Products
T1  - Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level
VL  - 186
DO  - 10.1016/j.indcrop.2022.115146
SP  - 115146
ER  - 

@article{
author = "Filipović, Biljana and Skorić, Marijana and Gašić, Uroš and Dragićević, Milan and Božunović, Jelena and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Šiler, Branislav and Bohanec, Borut and Milutinović, Milica and Mišić, Danijela",
year = "2022",
abstract = "Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.",
publisher = "Amsterdam : Elsevier",
journal = "Industrial Crops and Products",
title = "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level",
volume = "186",
doi = "10.1016/j.indcrop.2022.115146",
pages = "115146"
}

Filipović, B., Skorić, M., Gašić, U., Dragićević, M., Božunović, J., Matekalo, D., Nestorović Živković, J., Banjanac, T., Šiler, B., Bohanec, B., Milutinović, M.,& Mišić, D.. (2022). Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products
Amsterdam : Elsevier., 186, 115146.
https://doi.org/10.1016/j.indcrop.2022.115146

Filipović B, Skorić M, Gašić U, Dragićević M, Božunović J, Matekalo D, Nestorović Živković J, Banjanac T, Šiler B, Bohanec B, Milutinović M, Mišić D. Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products. 2022;186:115146.
doi:10.1016/j.indcrop.2022.115146 .

Filipović, Biljana, Skorić, Marijana, Gašić, Uroš, Dragićević, Milan, Božunović, Jelena, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Šiler, Branislav, Bohanec, Borut, Milutinović, Milica, Mišić, Danijela, "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level" in Industrial Crops and Products, 186 (2022):115146,
https://doi.org/10.1016/j.indcrop.2022.115146 . .

TY  - JOUR
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Živković, Suzana
AU  - Dragićević, Milan
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Petrović, Luka
AU  - Mišić, Danijela
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2022.914138/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9260424
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5073
AB  - Secoiridoid glucosides (SGs) are monoterpenoids derived from the iridoid cyclopentane-C-pyran skeleton with β-D glucose linked at C1 position. Coordinated metabolic processes, such as biosynthesis and catabolism of SGs, ensure constitutive presence of these bitter tasting compounds in plant tissues, which plays a decisive role in the defense against pathogens and herbivores. These compounds are susceptible to hydrolysis mediated by enzymes β-glucosidases, and the resulting aglycones are subsequently directed toward different metabolic pathways in plants. Function of two β-D-glucosidases (named CeBGlu1 and CeBGlu2) from centaury (Centaurium erythraea Rafn; fam. Gentianaceae), belonging to the glycoside hydrolase 1 (GH1) family, was confirmed using in vitro assays with recombinant proteins, following their heterologous expression in E. coli and His-tag affinity purification. Although they show slightly differential substrate preference, both isoforms display high specificity toward SGs and the organ-specific distribution of transcripts was positively correlated with the content of SGs in diploid and tetraploid C. erythraea plants. Transient overexpression of CeBGlu1 and CeBGlu2 in C. erythraea leaves induced changes in metabolite profiles. The effectiveness of transgene overexpression has been altered by plant ploidy. UHPLC/DAD/(±)HESI - MS2 profiling of leaves of diploid and tetraploid C. erythraea genotypes revealed that the amounts of major SGs; sweroside, swertiamarin, and gentiopicrin was decreased in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. The work demonstrates that in planta metabolic engineering adopting transient overexpression of CeBGlu1 and CeBGlu2 is a suitable tool for the modulation of SGs content and glucosides/aglycones ratio, which might have substantial effects on overall phytochemistry of C. erythraea.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Plant Science
T1  - Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.
VL  - 13
DO  - 10.3389/fpls.2022.914138
SP  - 914138
ER  - 

@article{
author = "Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Skorić, Marijana and Matekalo, Dragana and Živković, Suzana and Dragićević, Milan and Filipović, Biljana and Banjanac, Tijana and Petrović, Luka and Mišić, Danijela",
year = "2022",
abstract = "Secoiridoid glucosides (SGs) are monoterpenoids derived from the iridoid cyclopentane-C-pyran skeleton with β-D glucose linked at C1 position. Coordinated metabolic processes, such as biosynthesis and catabolism of SGs, ensure constitutive presence of these bitter tasting compounds in plant tissues, which plays a decisive role in the defense against pathogens and herbivores. These compounds are susceptible to hydrolysis mediated by enzymes β-glucosidases, and the resulting aglycones are subsequently directed toward different metabolic pathways in plants. Function of two β-D-glucosidases (named CeBGlu1 and CeBGlu2) from centaury (Centaurium erythraea Rafn; fam. Gentianaceae), belonging to the glycoside hydrolase 1 (GH1) family, was confirmed using in vitro assays with recombinant proteins, following their heterologous expression in E. coli and His-tag affinity purification. Although they show slightly differential substrate preference, both isoforms display high specificity toward SGs and the organ-specific distribution of transcripts was positively correlated with the content of SGs in diploid and tetraploid C. erythraea plants. Transient overexpression of CeBGlu1 and CeBGlu2 in C. erythraea leaves induced changes in metabolite profiles. The effectiveness of transgene overexpression has been altered by plant ploidy. UHPLC/DAD/(±)HESI - MS2 profiling of leaves of diploid and tetraploid C. erythraea genotypes revealed that the amounts of major SGs; sweroside, swertiamarin, and gentiopicrin was decreased in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. The work demonstrates that in planta metabolic engineering adopting transient overexpression of CeBGlu1 and CeBGlu2 is a suitable tool for the modulation of SGs content and glucosides/aglycones ratio, which might have substantial effects on overall phytochemistry of C. erythraea.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Plant Science",
title = "Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.",
volume = "13",
doi = "10.3389/fpls.2022.914138",
pages = "914138"
}

Božunović, J., Milutinović, M., Aničić, N., Skorić, M., Matekalo, D., Živković, S., Dragićević, M., Filipović, B., Banjanac, T., Petrović, L.,& Mišić, D.. (2022). Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.. in Frontiers in Plant Science
Lausanne: Frontiers Media S.A.., 13, 914138.
https://doi.org/10.3389/fpls.2022.914138

Božunović J, Milutinović M, Aničić N, Skorić M, Matekalo D, Živković S, Dragićević M, Filipović B, Banjanac T, Petrović L, Mišić D. Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.. in Frontiers in Plant Science. 2022;13:914138.
doi:10.3389/fpls.2022.914138 .

Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Skorić, Marijana, Matekalo, Dragana, Živković, Suzana, Dragićević, Milan, Filipović, Biljana, Banjanac, Tijana, Petrović, Luka, Mišić, Danijela, "Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn." in Frontiers in Plant Science, 13 (2022):914138,
https://doi.org/10.3389/fpls.2022.914138 . .

TY  - JOUR
AU  - Filipović, Biljana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Dragićević, Milan
AU  - Božunović, Jelena
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Bohanec, Borut
AU  - Milutinović, Milica
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5000
AB  - Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.
PB  - Amsterdam : Elsevier
T2  - Industrial Crops and Products
T1  - Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level
VL  - 186
DO  - 10.1016/j.indcrop.2022.115146
SP  - 115146
ER  - 

@article{
author = "Filipović, Biljana and Skorić, Marijana and Gašić, Uroš and Dragićević, Milan and Božunović, Jelena and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Šiler, Branislav and Bohanec, Borut and Milutinović, Milica and Mišić, Danijela",
year = "2022",
abstract = "Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.",
publisher = "Amsterdam : Elsevier",
journal = "Industrial Crops and Products",
title = "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level",
volume = "186",
doi = "10.1016/j.indcrop.2022.115146",
pages = "115146"
}

Filipović, B., Skorić, M., Gašić, U., Dragićević, M., Božunović, J., Matekalo, D., Nestorović Živković, J., Banjanac, T., Šiler, B., Bohanec, B., Milutinović, M.,& Mišić, D.. (2022). Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products
Amsterdam : Elsevier., 186, 115146.
https://doi.org/10.1016/j.indcrop.2022.115146

Filipović B, Skorić M, Gašić U, Dragićević M, Božunović J, Matekalo D, Nestorović Živković J, Banjanac T, Šiler B, Bohanec B, Milutinović M, Mišić D. Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products. 2022;186:115146.
doi:10.1016/j.indcrop.2022.115146 .

Filipović, Biljana, Skorić, Marijana, Gašić, Uroš, Dragićević, Milan, Božunović, Jelena, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Šiler, Branislav, Bohanec, Borut, Milutinović, Milica, Mišić, Danijela, "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level" in Industrial Crops and Products, 186 (2022):115146,
https://doi.org/10.1016/j.indcrop.2022.115146 . .

TY  - CONF
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Živković, Suzana
AU  - Dragićević, Milan
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Filipović, Biljana
AU  - Petrović, Luka
AU  - Banjanac, Tijana
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5189
AB  - Centaurium erythraea Rafn, also known as common centaury, is a rich source of pharmacologically active specialized metabolites among which secoiridoid glucosides (SG) predominate. These compounds belong to the group of monoterpenoids, which are derived from the iridoid cyclopentan-C-pyran skeleton with β-D glucose linked at C1 position. Major SGs of C. erythraea (sweroside, swertiamarin, and gentiopicrin) are biosynthesized through general iridoid pathway via secologanin, and it has been recently proved that their catabolism starts with the deglycosylation step catalyzed by beta-glucosidases (BGlu). Two BGlu genes from C. erythraea (CeBGlu1 and CeBGlu2), with previously confirmed function, were transiently overexpressed in leaves of five month-old diploid and tetraploid centaury plants. Agroinfiltration of Agrobacterium tumefaciens GV3101 strain harboring genes of interest in TMV-based overexpression pJL-TRBO vector (pJL-TR-BO:CeBGlu1; pJL-TRBO:CeBGlu2) resulted in transient in planta overexpression of CeBGlu1 and Ce-BGlu2. Metabolic profiling of secoiridoids in leaves of diploid and tetraploid C. erythraea genotypes revealed that amounts of sweroside, swertiamarin, and gentiopicrin, were significantly reduced in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. Diploid plants displayed more pronounced decrease in SGs content than tetraploids. The effectiveness of gene overexpression was obviously altered by plant ploidy, and was under the control of post-transcriptional gene silencing mechanisms in the infiltrated tissues. Transgenes are more prone to transcriptional inactivation in C. erythraea tetraploids than in diploids. In conclusion, SGs-specific β-glucosidases could serve as a molecular target of biotechnological interest, in order to shape SG profiles of centaury and related economically important
species of the Gentianaceae family.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5189
ER  - 

@conference{
author = "Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Živković, Suzana and Dragićević, Milan and Skorić, Marijana and Matekalo, Dragana and Filipović, Biljana and Petrović, Luka and Banjanac, Tijana and Mišić, Danijela",
year = "2022",
abstract = "Centaurium erythraea Rafn, also known as common centaury, is a rich source of pharmacologically active specialized metabolites among which secoiridoid glucosides (SG) predominate. These compounds belong to the group of monoterpenoids, which are derived from the iridoid cyclopentan-C-pyran skeleton with β-D glucose linked at C1 position. Major SGs of C. erythraea (sweroside, swertiamarin, and gentiopicrin) are biosynthesized through general iridoid pathway via secologanin, and it has been recently proved that their catabolism starts with the deglycosylation step catalyzed by beta-glucosidases (BGlu). Two BGlu genes from C. erythraea (CeBGlu1 and CeBGlu2), with previously confirmed function, were transiently overexpressed in leaves of five month-old diploid and tetraploid centaury plants. Agroinfiltration of Agrobacterium tumefaciens GV3101 strain harboring genes of interest in TMV-based overexpression pJL-TRBO vector (pJL-TR-BO:CeBGlu1; pJL-TRBO:CeBGlu2) resulted in transient in planta overexpression of CeBGlu1 and Ce-BGlu2. Metabolic profiling of secoiridoids in leaves of diploid and tetraploid C. erythraea genotypes revealed that amounts of sweroside, swertiamarin, and gentiopicrin, were significantly reduced in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. Diploid plants displayed more pronounced decrease in SGs content than tetraploids. The effectiveness of gene overexpression was obviously altered by plant ploidy, and was under the control of post-transcriptional gene silencing mechanisms in the infiltrated tissues. Transgenes are more prone to transcriptional inactivation in C. erythraea tetraploids than in diploids. In conclusion, SGs-specific β-glucosidases could serve as a molecular target of biotechnological interest, in order to shape SG profiles of centaury and related economically important
species of the Gentianaceae family.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5189"
}

Božunović, J., Milutinović, M., Aničić, N., Živković, S., Dragićević, M., Skorić, M., Matekalo, D., Filipović, B., Petrović, L., Banjanac, T.,& Mišić, D.. (2022). Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 109.
https://hdl.handle.net/21.15107/rcub_ibiss_5189

Božunović J, Milutinović M, Aničić N, Živković S, Dragićević M, Skorić M, Matekalo D, Filipović B, Petrović L, Banjanac T, Mišić D. Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:109.
https://hdl.handle.net/21.15107/rcub_ibiss_5189 .

Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Živković, Suzana, Dragićević, Milan, Skorić, Marijana, Matekalo, Dragana, Filipović, Biljana, Petrović, Luka, Banjanac, Tijana, Mišić, Danijela, "Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):109,
https://hdl.handle.net/21.15107/rcub_ibiss_5189 .

TY  - CHAP
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Skorić, Marijana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5761
AB  - Asplenium ceterach L. (family Aspleniaceae) is a perennial herbaceous fern. The lower surface of its fronds is covered with scales, which over time mature to a rusty color, giving the plant a characteristic appearance, hence the common name “rustyback” fern. It inhabits almost all of Europe, including the Mediterranean region, and Central Asia to the Himalayas, usually occurring in cracks in rocks. Rustyback fern is belonging to resurrection plants due to its ability to withstand desiccation and consequently completely recover after rehydration. It is thought that high concentrations of phenolic acids (mainly chlorogenic acid and caffeic acid) enable A. ceterach to reduce the damage of reactive oxygen species formed during dehydration. In addition to phenolic acids, the presence of other phenolic compounds is detected in A. ceterach including flavonoids, tannins, coumarins, and quinones. Phytochemical studies have shown that these phenolic compounds are mainly found in sporophytes of rustyback fern, while A. ceterach gametophyte is characterized predominantly by xanthones, primarily mangiferin and its glycoside. Additionally, rustyback fern represents a rich source of different volatile organic compounds, mainly lipid derivatives. Owing to the presence of various bioactive metabolites, it is not surprising that A. ceterach has long been known for its healing properties, and numerous studies have proven its various biological activities, including antimicrobial, antioxidant, cytotoxic, anticancer, diuretic, as well as anti-inflammatory properties.
PB  - Cham: Springer
T2  - Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry
T1  - Phytochemicals and Biological Activities of Asplenium ceterach
DO  - 10.1007/978-3-030-97415-2_19-1
ER  - 

@inbook{
author = "Živković, Suzana and Milutinović, Milica and Skorić, Marijana",
year = "2022",
abstract = "Asplenium ceterach L. (family Aspleniaceae) is a perennial herbaceous fern. The lower surface of its fronds is covered with scales, which over time mature to a rusty color, giving the plant a characteristic appearance, hence the common name “rustyback” fern. It inhabits almost all of Europe, including the Mediterranean region, and Central Asia to the Himalayas, usually occurring in cracks in rocks. Rustyback fern is belonging to resurrection plants due to its ability to withstand desiccation and consequently completely recover after rehydration. It is thought that high concentrations of phenolic acids (mainly chlorogenic acid and caffeic acid) enable A. ceterach to reduce the damage of reactive oxygen species formed during dehydration. In addition to phenolic acids, the presence of other phenolic compounds is detected in A. ceterach including flavonoids, tannins, coumarins, and quinones. Phytochemical studies have shown that these phenolic compounds are mainly found in sporophytes of rustyback fern, while A. ceterach gametophyte is characterized predominantly by xanthones, primarily mangiferin and its glycoside. Additionally, rustyback fern represents a rich source of different volatile organic compounds, mainly lipid derivatives. Owing to the presence of various bioactive metabolites, it is not surprising that A. ceterach has long been known for its healing properties, and numerous studies have proven its various biological activities, including antimicrobial, antioxidant, cytotoxic, anticancer, diuretic, as well as anti-inflammatory properties.",
publisher = "Cham: Springer",
journal = "Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry",
booktitle = "Phytochemicals and Biological Activities of Asplenium ceterach",
doi = "10.1007/978-3-030-97415-2_19-1"
}

Živković, S., Milutinović, M.,& Skorić, M.. (2022). Phytochemicals and Biological Activities of Asplenium ceterach. in Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry
Cham: Springer..
https://doi.org/10.1007/978-3-030-97415-2_19-1

Živković S, Milutinović M, Skorić M. Phytochemicals and Biological Activities of Asplenium ceterach. in Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry. 2022;.
doi:10.1007/978-3-030-97415-2_19-1 .

Živković, Suzana, Milutinović, Milica, Skorić, Marijana, "Phytochemicals and Biological Activities of Asplenium ceterach" in Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry (2022),
https://doi.org/10.1007/978-3-030-97415-2_19-1 . .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragićević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Perišić, Mirjana
AU  - Mišić, Danijela
PY  - 2021
UR  - https://doi.org/10.3390/plants10010142
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4232
AB  - Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.
PB  - MDPI AG
T2  - Plants
T1  - Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro
IS  - 1
VL  - 10
DO  - 10.3390/plants10010142
SP  - 1
EP  - 20
ER  - 

@article{
author = "Dmitrović, Slavica and Dragićević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Perišić, Mirjana and Mišić, Danijela",
year = "2021",
abstract = "Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.",
publisher = "MDPI AG",
journal = "Plants",
title = "Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro",
number = "1",
volume = "10",
doi = "10.3390/plants10010142",
pages = "1-20"
}

Dmitrović, S., Dragićević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D., Perišić, M.,& Mišić, D.. (2021). Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro. in Plants
MDPI AG., 10(1), 1-20.
https://doi.org/10.3390/plants10010142

Dmitrović S, Dragićević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Perišić M, Mišić D. Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro. in Plants. 2021;10(1):1-20.
doi:10.3390/plants10010142 .

Dmitrović, Slavica, Dragićević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Perišić, Mirjana, Mišić, Danijela, "Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro" in Plants, 10, no. 1 (2021):1-20,
https://doi.org/10.3390/plants10010142 . .

TY  - JOUR
AU  - Aničić, Neda
AU  - Patelou, Efstathia
AU  - Papanikolaou, Antigoni
AU  - Kanioura, Anthi
AU  - Valdesturli, Camilla
AU  - Arapitsas, Panagiotis
AU  - Skorić, Marijana
AU  - Dragićević, Milan
AU  - Gašić, Uroš
AU  - Koukounaras, Athanasios
AU  - Kostas, Stefanos
AU  - Sarrou, Eirini
AU  - Martens, Stefan
AU  - Mišić, Danijela
AU  - Kanellis, Angelos
PY  - 2021
UR  - www.frontiersin.org
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4219
AB  - Cistus creticus L. subsp. creticus (rockrose) is a shrub widespread in Greece and the Mediterranean basin and has been used in traditional medicine as herb tea for colds, for healing and digestive hitches, for the treatment of maladies, as perfumes, and for other purposes. Compounds from its flavonoid fraction have recently drawn attention due to antiviral action against influenza virus and HIV. Although several bioactive metabolites belonging to this group have been chemically characterized in the leaves, the genes involved in their biosynthesis in Cistus remain largely unknown. Flavonoid metabolism during C. creticus fruit development was studied by adopting comparative metabolomic and transcriptomic approaches. The present study highlights the fruit of C. creticus subsp. creticus as a rich source of flavonols, flavan-3-ols, and proanthocyanidins, all of which displayed a decreasing trend during fruit development. The majority of proanthocyanidins recorded in Cistus fruit are B-type procyanidins and prodelphinidins, while gallocatechin and catechin are the dominant flavan-3-ols. The expression patterns of biosynthetic genes and transcription factors were analyzed in flowers and throughout three fruit development stages. Flavonoid biosynthetic genes were developmentally regulated, showing a decrease in transcript levels during fruit maturation. A high degree of positive correlations between the content of targeted metabolites and the expression of biosynthetic genes indicated the transcriptional regulation of flavonoid biosynthesis during C. creticus fruit development. This is further supported by the high degree of significant positive correlations between the expression of biosynthetic genes and transcription factors. The results suggest that leucoanthocyanidin reductase predominates the biosynthetic pathway in the control of flavan-3-ol formation, which results in catechin and gallocatechin as two of the major building blocks for Cistus proanthocyanidins. Additionally, there is a decline in ethylene production rates during non-climacteric Cistus fruit maturation, which coincides with the downregulation of the majority of flavonoid- and ethylene-related biosynthetic genes and corresponding transcription factors as well as with the decline in flavonoid content. Finally, functional characterization of a Cistus flavonoid hydroxylase (F3′5′H) was performed for the first time.
PB  - Frontiers Media S.A.
T2  - Frontiers in Plant Science
T1  - Comparative Metabolite and Gene Expression Analyses in Combination With Gene Characterization Revealed the Patterns of Flavonoid Accumulation During Cistus creticus subsp. creticus Fruit Development
VL  - 12
DO  - 10.3389/fpls.2021.619634
SP  - 385
ER  - 

@article{
author = "Aničić, Neda and Patelou, Efstathia and Papanikolaou, Antigoni and Kanioura, Anthi and Valdesturli, Camilla and Arapitsas, Panagiotis and Skorić, Marijana and Dragićević, Milan and Gašić, Uroš and Koukounaras, Athanasios and Kostas, Stefanos and Sarrou, Eirini and Martens, Stefan and Mišić, Danijela and Kanellis, Angelos",
year = "2021",
abstract = "Cistus creticus L. subsp. creticus (rockrose) is a shrub widespread in Greece and the Mediterranean basin and has been used in traditional medicine as herb tea for colds, for healing and digestive hitches, for the treatment of maladies, as perfumes, and for other purposes. Compounds from its flavonoid fraction have recently drawn attention due to antiviral action against influenza virus and HIV. Although several bioactive metabolites belonging to this group have been chemically characterized in the leaves, the genes involved in their biosynthesis in Cistus remain largely unknown. Flavonoid metabolism during C. creticus fruit development was studied by adopting comparative metabolomic and transcriptomic approaches. The present study highlights the fruit of C. creticus subsp. creticus as a rich source of flavonols, flavan-3-ols, and proanthocyanidins, all of which displayed a decreasing trend during fruit development. The majority of proanthocyanidins recorded in Cistus fruit are B-type procyanidins and prodelphinidins, while gallocatechin and catechin are the dominant flavan-3-ols. The expression patterns of biosynthetic genes and transcription factors were analyzed in flowers and throughout three fruit development stages. Flavonoid biosynthetic genes were developmentally regulated, showing a decrease in transcript levels during fruit maturation. A high degree of positive correlations between the content of targeted metabolites and the expression of biosynthetic genes indicated the transcriptional regulation of flavonoid biosynthesis during C. creticus fruit development. This is further supported by the high degree of significant positive correlations between the expression of biosynthetic genes and transcription factors. The results suggest that leucoanthocyanidin reductase predominates the biosynthetic pathway in the control of flavan-3-ol formation, which results in catechin and gallocatechin as two of the major building blocks for Cistus proanthocyanidins. Additionally, there is a decline in ethylene production rates during non-climacteric Cistus fruit maturation, which coincides with the downregulation of the majority of flavonoid- and ethylene-related biosynthetic genes and corresponding transcription factors as well as with the decline in flavonoid content. Finally, functional characterization of a Cistus flavonoid hydroxylase (F3′5′H) was performed for the first time.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Plant Science",
title = "Comparative Metabolite and Gene Expression Analyses in Combination With Gene Characterization Revealed the Patterns of Flavonoid Accumulation During Cistus creticus subsp. creticus Fruit Development",
volume = "12",
doi = "10.3389/fpls.2021.619634",
pages = "385"
}

Aničić, N., Patelou, E., Papanikolaou, A., Kanioura, A., Valdesturli, C., Arapitsas, P., Skorić, M., Dragićević, M., Gašić, U., Koukounaras, A., Kostas, S., Sarrou, E., Martens, S., Mišić, D.,& Kanellis, A.. (2021). Comparative Metabolite and Gene Expression Analyses in Combination With Gene Characterization Revealed the Patterns of Flavonoid Accumulation During Cistus creticus subsp. creticus Fruit Development. in Frontiers in Plant Science
Frontiers Media S.A.., 12, 385.
https://doi.org/10.3389/fpls.2021.619634

Aničić N, Patelou E, Papanikolaou A, Kanioura A, Valdesturli C, Arapitsas P, Skorić M, Dragićević M, Gašić U, Koukounaras A, Kostas S, Sarrou E, Martens S, Mišić D, Kanellis A. Comparative Metabolite and Gene Expression Analyses in Combination With Gene Characterization Revealed the Patterns of Flavonoid Accumulation During Cistus creticus subsp. creticus Fruit Development. in Frontiers in Plant Science. 2021;12:385.
doi:10.3389/fpls.2021.619634 .

Aničić, Neda, Patelou, Efstathia, Papanikolaou, Antigoni, Kanioura, Anthi, Valdesturli, Camilla, Arapitsas, Panagiotis, Skorić, Marijana, Dragićević, Milan, Gašić, Uroš, Koukounaras, Athanasios, Kostas, Stefanos, Sarrou, Eirini, Martens, Stefan, Mišić, Danijela, Kanellis, Angelos, "Comparative Metabolite and Gene Expression Analyses in Combination With Gene Characterization Revealed the Patterns of Flavonoid Accumulation During Cistus creticus subsp. creticus Fruit Development" in Frontiers in Plant Science, 12 (2021):385,
https://doi.org/10.3389/fpls.2021.619634 . .

TY  - JOUR
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ristić, Mihailo
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Perišić, Mirjana
AU  - Puač, Nevena
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4394
AB  - When exposed to stressful conditions, plants produce numerous volatile organic compounds (VOCs) that have different biological and environmental functions. VOCs emitted during the rehydration process by the fronds of desiccation tolerant fern Asplenium ceterach L. were investigated. Headspace GC–MS analysis revealed that the volatiles profile of rustyback fern is mainly composed of fatty acid derivatives: isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols, and related compounds. Aerial parts of the rustyback fern do not contain monoterpene-type, sesquiterpene-type, and diterpene-type hydrocarbons or corresponding terpenoids. Online detection of VOCs using proton-transfer reaction mass spectrometry (PTR–MS) showed a significant increase in emission intensity of dominant volatiles during the first hours of the rehydration process. Twelve hours after re-watering, emission of detected volatiles had returned to the basal levels that corresponded to hydrated plants. During the early phase of rehydration malondialdehyde (MDA) content in fronds, as an indicator of membrane damage, decreased rapidly which implies that lipoxygenase activity is not stimulated during the recovery process of rustyback fern.
PB  - Basel: MDPI
T2  - Biology
T1  - Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds
IS  - 7
VL  - 10
DO  - 10.3390/biology10070574
SP  - 574
ER  - 

@article{
author = "Živković, Suzana and Skorić, Marijana and Ristić, Mihailo and Filipović, Biljana and Milutinović, Milica and Perišić, Mirjana and Puač, Nevena",
year = "2021",
abstract = "When exposed to stressful conditions, plants produce numerous volatile organic compounds (VOCs) that have different biological and environmental functions. VOCs emitted during the rehydration process by the fronds of desiccation tolerant fern Asplenium ceterach L. were investigated. Headspace GC–MS analysis revealed that the volatiles profile of rustyback fern is mainly composed of fatty acid derivatives: isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols, and related compounds. Aerial parts of the rustyback fern do not contain monoterpene-type, sesquiterpene-type, and diterpene-type hydrocarbons or corresponding terpenoids. Online detection of VOCs using proton-transfer reaction mass spectrometry (PTR–MS) showed a significant increase in emission intensity of dominant volatiles during the first hours of the rehydration process. Twelve hours after re-watering, emission of detected volatiles had returned to the basal levels that corresponded to hydrated plants. During the early phase of rehydration malondialdehyde (MDA) content in fronds, as an indicator of membrane damage, decreased rapidly which implies that lipoxygenase activity is not stimulated during the recovery process of rustyback fern.",
publisher = "Basel: MDPI",
journal = "Biology",
title = "Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds",
number = "7",
volume = "10",
doi = "10.3390/biology10070574",
pages = "574"
}

Živković, S., Skorić, M., Ristić, M., Filipović, B., Milutinović, M., Perišić, M.,& Puač, N.. (2021). Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds. in Biology
Basel: MDPI., 10(7), 574.
https://doi.org/10.3390/biology10070574

Živković S, Skorić M, Ristić M, Filipović B, Milutinović M, Perišić M, Puač N. Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds. in Biology. 2021;10(7):574.
doi:10.3390/biology10070574 .

Živković, Suzana, Skorić, Marijana, Ristić, Mihailo, Filipović, Biljana, Milutinović, Milica, Perišić, Mirjana, Puač, Nevena, "Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds" in Biology, 10, no. 7 (2021):574,
https://doi.org/10.3390/biology10070574 . .

TY  - THES
AU  - Aničić, Neda
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/123456789/3895
AB  - Specijalizovani metabolizam roda Nepeta (Lamiaceae) karakteriše monoterpenoidni iridoid nepetalakton (NL), koji se javlja u vidu 4 diastereoizomera sa 7S konfiguracijom. Iako se dosta zna o biološkim aktivnostima NL, nedovoljno je istražen njegov metabolizam, kao i uloga u biljkama. Ova doktorska disertacija po prvi put opisuje mehanizme regulacije biosinteze NL tokom dehidratacije u listovima vrsta Nepeta rtanjensis Diklić & Milojević i N. argolica Bory & Chaub subsp. argolica, koje su adaptirane na kserotermna staništa. Uprkos koordinisanom sniženju ekspresije kandidata 10 gena biosintetskog puta iridoida i redukovanoj metaboličkoj aktivnosti, ove vrste su razvile mehanizme za očuvanje nivoa NL u uslovima vodnog deficita. U ovim mehanizmima značajnu ulogu imaju transkripcioni faktori MYC2 i YABBY5 kao pozitivni regulatori biosinteze NL, snižen intenzitet katabolizma NL, kao i očuvanje morfologije glandularnih trihoma, koji predstavljaju glavno mesto biosinteze i akumulacije NL. Iridoid sintaze, čija je funkcija potvrđena enzimatskim in vitro testovima sa rekombinantim proteinima eksprimiranim u heterologom domaćinu Escherichia coli, eksprimiraju se kako kod N. rtanjensis (NrIS2) i N. sibirica L. (NsIS), koje produkuju NL, tako i kod N. nervosa L. (NnIS) koja se odlikuje odsustvom merljivih količina NL u tkivima. N. nervosa očigledno poseduje biosintetsku platformu za produkciju iridoida, koja je neaktivna, najverovatnije usled „gašenja” ili „stišavanja” funkcije nekog gena uzvodno u biosintetskom putu. Rasvetljavanjem mehanizama regulacije metabolizma NL kod vrsta roda Nepeta date su smernice za buduću primenu metaboličkog inženjeringa u cilju optimizacije proizvodnje NL, dok je N. nervosa izdvojena kao interesantna model vrsta za ova istraživanja.
AB  - The specialized metabolism of the genus Nepeta (Lamiaceae) is distinguished by the monoterpenoid iridoid nepetalactone (NL), which exists in the form of 4 diastereoisomers with a 7S configuration. Although its biological activities are well-studied, the NL metabolism, as well as its role in plants, is not well understood. Results of this doctoral thesis describe the regulatory mechanisms determining NL biosynthesis during dehydration, in leaves of Nepeta rtanjensis Diklić & Milojević and N. argolica Bory & Chaub subsp. argolica, which are adapted to xerothermic habitats. Despite a coordinated decrease in the expression of 10 candidate iridoid-related biosynthetic genes and reduced metabolic activity, these species have developed mechanisms to preserve NL levels under water deficit conditions. These mechanisms include the significant role of MYC2 and YABBY5 transcription factors as positive regulators of NL biosynthesis, reduced intensity of NL catabolism, and preservation of the morphology of glandular trichomes – the major site of NL biosynthesis and accumulation. Iridoid synthases, functionaly characterized by in vitro enzymatic assays using recombinant proteins heterologously expressed in Escherichia coli, are expressed in NL-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in N. nervosa L. (NnIS), which is characterized by the absence of NL in tissues. N. nervosa probably possesses inactive iridoid biosynthetic platform, most likely due to the "switching off" or "silencing" of some genes upstream in the pathway. Elucidation of the regulatory mechanisms behind NL metabolism in Nepeta, provides the background for future metabolic engineering of NL production, and highlightes N. nervosa as an interesting model species for future studies.
PB  - Belgrade: Faculty of Biology, University of Belgrade
T2  - Faculty of Biology, University of Belgrade
T1  - Metabolizam nepetalaktona u listovima odabranih vrsta roda Nepeta (Lamiaceae) i njegova regulacija tokom dehidratacije
T1  - Metabolism of nepetalactone in leaves of selected species of the genus Nepeta (Lamiaceae) and its regulation during dehydration
SP  - 1
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3895
ER  - 

@phdthesis{
author = "Aničić, Neda",
year = "2020",
abstract = "Specijalizovani metabolizam roda Nepeta (Lamiaceae) karakteriše monoterpenoidni iridoid nepetalakton (NL), koji se javlja u vidu 4 diastereoizomera sa 7S konfiguracijom. Iako se dosta zna o biološkim aktivnostima NL, nedovoljno je istražen njegov metabolizam, kao i uloga u biljkama. Ova doktorska disertacija po prvi put opisuje mehanizme regulacije biosinteze NL tokom dehidratacije u listovima vrsta Nepeta rtanjensis Diklić & Milojević i N. argolica Bory & Chaub subsp. argolica, koje su adaptirane na kserotermna staništa. Uprkos koordinisanom sniženju ekspresije kandidata 10 gena biosintetskog puta iridoida i redukovanoj metaboličkoj aktivnosti, ove vrste su razvile mehanizme za očuvanje nivoa NL u uslovima vodnog deficita. U ovim mehanizmima značajnu ulogu imaju transkripcioni faktori MYC2 i YABBY5 kao pozitivni regulatori biosinteze NL, snižen intenzitet katabolizma NL, kao i očuvanje morfologije glandularnih trihoma, koji predstavljaju glavno mesto biosinteze i akumulacije NL. Iridoid sintaze, čija je funkcija potvrđena enzimatskim in vitro testovima sa rekombinantim proteinima eksprimiranim u heterologom domaćinu Escherichia coli, eksprimiraju se kako kod N. rtanjensis (NrIS2) i N. sibirica L. (NsIS), koje produkuju NL, tako i kod N. nervosa L. (NnIS) koja se odlikuje odsustvom merljivih količina NL u tkivima. N. nervosa očigledno poseduje biosintetsku platformu za produkciju iridoida, koja je neaktivna, najverovatnije usled „gašenja” ili „stišavanja” funkcije nekog gena uzvodno u biosintetskom putu. Rasvetljavanjem mehanizama regulacije metabolizma NL kod vrsta roda Nepeta date su smernice za buduću primenu metaboličkog inženjeringa u cilju optimizacije proizvodnje NL, dok je N. nervosa izdvojena kao interesantna model vrsta za ova istraživanja., The specialized metabolism of the genus Nepeta (Lamiaceae) is distinguished by the monoterpenoid iridoid nepetalactone (NL), which exists in the form of 4 diastereoisomers with a 7S configuration. Although its biological activities are well-studied, the NL metabolism, as well as its role in plants, is not well understood. Results of this doctoral thesis describe the regulatory mechanisms determining NL biosynthesis during dehydration, in leaves of Nepeta rtanjensis Diklić & Milojević and N. argolica Bory & Chaub subsp. argolica, which are adapted to xerothermic habitats. Despite a coordinated decrease in the expression of 10 candidate iridoid-related biosynthetic genes and reduced metabolic activity, these species have developed mechanisms to preserve NL levels under water deficit conditions. These mechanisms include the significant role of MYC2 and YABBY5 transcription factors as positive regulators of NL biosynthesis, reduced intensity of NL catabolism, and preservation of the morphology of glandular trichomes – the major site of NL biosynthesis and accumulation. Iridoid synthases, functionaly characterized by in vitro enzymatic assays using recombinant proteins heterologously expressed in Escherichia coli, are expressed in NL-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in N. nervosa L. (NnIS), which is characterized by the absence of NL in tissues. N. nervosa probably possesses inactive iridoid biosynthetic platform, most likely due to the "switching off" or "silencing" of some genes upstream in the pathway. Elucidation of the regulatory mechanisms behind NL metabolism in Nepeta, provides the background for future metabolic engineering of NL production, and highlightes N. nervosa as an interesting model species for future studies.",
publisher = "Belgrade: Faculty of Biology, University of Belgrade",
journal = "Faculty of Biology, University of Belgrade",
title = "Metabolizam nepetalaktona u listovima odabranih vrsta roda Nepeta (Lamiaceae) i njegova regulacija tokom dehidratacije, Metabolism of nepetalactone in leaves of selected species of the genus Nepeta (Lamiaceae) and its regulation during dehydration",
pages = "1-147",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3895"
}

Aničić, N.. (2020). Metabolizam nepetalaktona u listovima odabranih vrsta roda Nepeta (Lamiaceae) i njegova regulacija tokom dehidratacije. in Faculty of Biology, University of Belgrade
Belgrade: Faculty of Biology, University of Belgrade., 1-147.
https://hdl.handle.net/21.15107/rcub_ibiss_3895

Aničić N. Metabolizam nepetalaktona u listovima odabranih vrsta roda Nepeta (Lamiaceae) i njegova regulacija tokom dehidratacije. in Faculty of Biology, University of Belgrade. 2020;:1-147.
https://hdl.handle.net/21.15107/rcub_ibiss_3895 .

Aničić, Neda, "Metabolizam nepetalaktona u listovima odabranih vrsta roda Nepeta (Lamiaceae) i njegova regulacija tokom dehidratacije" in Faculty of Biology, University of Belgrade (2020):1-147,
https://hdl.handle.net/21.15107/rcub_ibiss_3895 .

TY  - JOUR
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Petrović, Luka
AU  - Dragićević, Milan
AU  - Dmitrović, Slavica
AU  - Mišić, Danijela
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32172017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3626
AB  - A number of Nepeta species (fam. Lamiaceae) are interesting medicinal crops for arid and semi-arid areas, due to their ability to maintain essential developmental and physiological processes and to rationalize their specialized metabolism under water deficit growth conditions. The present research is, to our knowledge, the first attempt to investigate the molecular background of the dehydration-induced changes in specialized metabolism of Nepeta species, which will help to understand relations between dehydration stress on one hand and biomass production and yield of nepetalactone (NL) on the other. During the 6 days exposure of Nepeta rtanjensis Diklić & Milojević and Nepeta argolica Bory & Chaub. ssp. argolica plants to PEG-induced dehydration stress under experimental in vitro conditions, decrease in transcript levels of the majority of 10 NL biosynthetic genes, and some of the 5 transcription factors (TFs) were recorded, simultaneously with the initial reduction in NL content. The two model species evidently employ similar strategies in response to severe dehydration stress; however N. rtanjensis is highlighted as the species more efficient in maintaining NL amounts in tissues. The results suggest trichome-specific and co-ordinately regulated NL biosynthesis at the level of gene expression, with trichome enriched MYC2 and YABBY5 TFs being the potential positive regulators. Manipulation of such TFs can be effective for engineering the NL biosynthetic pathway, and for the increased production of cis,trans-NL in N. argolica ssp. argolica and trans,cis-NL in N. rtanjensis.
T2  - Phytochemistry
T1  - Alterations in nepetalactone metabolism during polyethylene glycol (PEG)-induced dehydration stress in two Nepeta species.
VL  - 174
DO  - 10.1016/j.phytochem.2020.112340
SP  - 112340
ER  - 

@article{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Nestorović Živković, Jasmina and Petrović, Luka and Dragićević, Milan and Dmitrović, Slavica and Mišić, Danijela",
year = "2020",
abstract = "A number of Nepeta species (fam. Lamiaceae) are interesting medicinal crops for arid and semi-arid areas, due to their ability to maintain essential developmental and physiological processes and to rationalize their specialized metabolism under water deficit growth conditions. The present research is, to our knowledge, the first attempt to investigate the molecular background of the dehydration-induced changes in specialized metabolism of Nepeta species, which will help to understand relations between dehydration stress on one hand and biomass production and yield of nepetalactone (NL) on the other. During the 6 days exposure of Nepeta rtanjensis Diklić & Milojević and Nepeta argolica Bory & Chaub. ssp. argolica plants to PEG-induced dehydration stress under experimental in vitro conditions, decrease in transcript levels of the majority of 10 NL biosynthetic genes, and some of the 5 transcription factors (TFs) were recorded, simultaneously with the initial reduction in NL content. The two model species evidently employ similar strategies in response to severe dehydration stress; however N. rtanjensis is highlighted as the species more efficient in maintaining NL amounts in tissues. The results suggest trichome-specific and co-ordinately regulated NL biosynthesis at the level of gene expression, with trichome enriched MYC2 and YABBY5 TFs being the potential positive regulators. Manipulation of such TFs can be effective for engineering the NL biosynthetic pathway, and for the increased production of cis,trans-NL in N. argolica ssp. argolica and trans,cis-NL in N. rtanjensis.",
journal = "Phytochemistry",
title = "Alterations in nepetalactone metabolism during polyethylene glycol (PEG)-induced dehydration stress in two Nepeta species.",
volume = "174",
doi = "10.1016/j.phytochem.2020.112340",
pages = "112340"
}

Aničić, N., Matekalo, D., Skorić, M., Nestorović Živković, J., Petrović, L., Dragićević, M., Dmitrović, S.,& Mišić, D.. (2020). Alterations in nepetalactone metabolism during polyethylene glycol (PEG)-induced dehydration stress in two Nepeta species.. in Phytochemistry, 174, 112340.
https://doi.org/10.1016/j.phytochem.2020.112340

Aničić N, Matekalo D, Skorić M, Nestorović Živković J, Petrović L, Dragićević M, Dmitrović S, Mišić D. Alterations in nepetalactone metabolism during polyethylene glycol (PEG)-induced dehydration stress in two Nepeta species.. in Phytochemistry. 2020;174:112340.
doi:10.1016/j.phytochem.2020.112340 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Nestorović Živković, Jasmina, Petrović, Luka, Dragićević, Milan, Dmitrović, Slavica, Mišić, Danijela, "Alterations in nepetalactone metabolism during polyethylene glycol (PEG)-induced dehydration stress in two Nepeta species." in Phytochemistry, 174 (2020):112340,
https://doi.org/10.1016/j.phytochem.2020.112340 . .

TY  - JOUR
AU  - Hadi, N
AU  - Shojaeiyan, A
AU  - Sefidkon, F
AU  - Jafari, A A
AU  - Mišić, Danijela
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
PY  - 2020
UR  - http://jast.modares.ac.ir/article-23-33509-en.html
UR  - https://jast.modares.ac.ir/article-23-33509-en.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3968
AB  - The genus Nepeta is one of the largest genera in the Lamiaceae family, and Iran is one of the main centers of origin of this genus. The genetic diversity estimation of the genus Nepeta germplasm provides a basis for future biodiversity conservation efforts and also for the selection of high-productive genotype(s) for the field production improvement. In this work, eleven RAPD primers with suitable banding pattern and prominent polymorphism were used for the estimation of infraspecific genetic diversity of N. kotschyi based on 21 populations. The study included 19 and 2 populations belonging to var. persica and var. kotschyi, respectively. Eleven primers amplified totally 225 scorable RAPD loci, 204 of which were polymorphic. The average number of bands per primer was 20.5, 18.5 of which were polymorphic. Primers OPF 05, OPB 15, OPT 14, OPO 07, and OPF 14 were the most powerful for the detection of the genetic diversity across the samples. Cluster analysis showed six genotypic groups. N. kotschyi var. kotschyi populations were placed in a group separated from the samples belonging to N. kotschyi var. persica. The same analysis showed that the genetic diversity pattern corresponds to the geographical distribution of the populations. Of the total variation in the studied germplasm, 32% was related to inter-populations and 68% to intra-populations variation. The results indicated the high potential of RAPD markers to resolve the status of the studied genotypes in regard to inter-and intra-populations diversity and to diversify N. kotschyi varieties previously resolved by morphometric methods.
PB  - Journal of Agricultural Science and Technology
T2  - Journal of Agricultural Science and Technology
T1  - Assessment of Infraspecific Genetic Diversity in Nepeta kotschyi Boiss., a Native Iranian Medicinal Plant
IS  - 5
VL  - 22
SP  - 1327
EP  - 1342
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3968
ER  - 

@article{
author = "Hadi, N and Shojaeiyan, A and Sefidkon, F and Jafari, A A and Mišić, Danijela and Banjanac, Tijana and Šiler, Branislav",
year = "2020",
abstract = "The genus Nepeta is one of the largest genera in the Lamiaceae family, and Iran is one of the main centers of origin of this genus. The genetic diversity estimation of the genus Nepeta germplasm provides a basis for future biodiversity conservation efforts and also for the selection of high-productive genotype(s) for the field production improvement. In this work, eleven RAPD primers with suitable banding pattern and prominent polymorphism were used for the estimation of infraspecific genetic diversity of N. kotschyi based on 21 populations. The study included 19 and 2 populations belonging to var. persica and var. kotschyi, respectively. Eleven primers amplified totally 225 scorable RAPD loci, 204 of which were polymorphic. The average number of bands per primer was 20.5, 18.5 of which were polymorphic. Primers OPF 05, OPB 15, OPT 14, OPO 07, and OPF 14 were the most powerful for the detection of the genetic diversity across the samples. Cluster analysis showed six genotypic groups. N. kotschyi var. kotschyi populations were placed in a group separated from the samples belonging to N. kotschyi var. persica. The same analysis showed that the genetic diversity pattern corresponds to the geographical distribution of the populations. Of the total variation in the studied germplasm, 32% was related to inter-populations and 68% to intra-populations variation. The results indicated the high potential of RAPD markers to resolve the status of the studied genotypes in regard to inter-and intra-populations diversity and to diversify N. kotschyi varieties previously resolved by morphometric methods.",
publisher = "Journal of Agricultural Science and Technology",
journal = "Journal of Agricultural Science and Technology",
title = "Assessment of Infraspecific Genetic Diversity in Nepeta kotschyi Boiss., a Native Iranian Medicinal Plant",
number = "5",
volume = "22",
pages = "1327-1342",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3968"
}

Hadi, N., Shojaeiyan, A., Sefidkon, F., Jafari, A. A., Mišić, D., Banjanac, T.,& Šiler, B.. (2020). Assessment of Infraspecific Genetic Diversity in Nepeta kotschyi Boiss., a Native Iranian Medicinal Plant. in Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology., 22(5), 1327-1342.
https://hdl.handle.net/21.15107/rcub_ibiss_3968

Hadi N, Shojaeiyan A, Sefidkon F, Jafari AA, Mišić D, Banjanac T, Šiler B. Assessment of Infraspecific Genetic Diversity in Nepeta kotschyi Boiss., a Native Iranian Medicinal Plant. in Journal of Agricultural Science and Technology. 2020;22(5):1327-1342.
https://hdl.handle.net/21.15107/rcub_ibiss_3968 .

Hadi, N, Shojaeiyan, A, Sefidkon, F, Jafari, A A, Mišić, Danijela, Banjanac, Tijana, Šiler, Branislav, "Assessment of Infraspecific Genetic Diversity in Nepeta kotschyi Boiss., a Native Iranian Medicinal Plant" in Journal of Agricultural Science and Technology, 22, no. 5 (2020):1327-1342,
https://hdl.handle.net/21.15107/rcub_ibiss_3968 .

TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Katanić Stanković, Jelena S.
AU  - Jurić, Tatjana
AU  - Srećković, Nikola
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Monti, Daria Maria
AU  - Imbimbo, Paola
AU  - Nikles, Stefanie
AU  - Pan, San-Po
AU  - Bauer, Rudolf
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0926669019309847?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3562
AB  - Blackstonia perfoliata (L.) Huds. is known as a highly bitter secoiridoid glycosides-containing plant and as a possible substitute for some Gentianaceae plants in herbal preparations. Nevertheless, its bioactive properties are still unknown. The present study aimed to characterize both, secoiridoid glycosides and phenolic constituents, and to investigate antioxidant, antimicrobial, anti-inflammatory, and cytotoxic activities of B. perfoliata methanolic extract. The secoiridoid glycosides swertiamarin, gentiopicrin, and sweroside were found to be the dominant compounds of the extract, while 23 phenolic compounds were identified in much lower concentrations. Among phenolics, flavanols were the most abundant, which represents a unique feature among Gentianaceae species. The extract showed moderate to weak antioxidant activity with better performance in inhibition of lipid peroxidation than in free radical scavenging activities. The extract showed generally better antifungal properties compared with its antibacterial potential. Also, B. perfoliata demonstrated in vitro anti-inflammatory activity, the extract (50 μg/mL) showed inhibition of cyclooxigenases, COX-1 and COX-2, activities (19.65 and 48.02%, respectively). It also displayed biocompatibility on the immortalized and cancer cells, as no cytotoxic effect was observed. For the first time, the bioactive potential of this species was demonstrated, justifying its usage in pharmaceutical and food products as an alternative for some overexploited and endangered species from the Gentianaceae family.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Blackstonia perfoliata (L.) Huds. (Gentianaceae): A promising source of useful bioactive compounds
VL  - 145
DO  - 10.1016/J.INDCROP.2019.111974
SP  - 111974
ER  - 

@article{
author = "Mihailović, Vladimir and Katanić Stanković, Jelena S. and Jurić, Tatjana and Srećković, Nikola and Mišić, Danijela and Šiler, Branislav and Monti, Daria Maria and Imbimbo, Paola and Nikles, Stefanie and Pan, San-Po and Bauer, Rudolf",
year = "2020",
abstract = "Blackstonia perfoliata (L.) Huds. is known as a highly bitter secoiridoid glycosides-containing plant and as a possible substitute for some Gentianaceae plants in herbal preparations. Nevertheless, its bioactive properties are still unknown. The present study aimed to characterize both, secoiridoid glycosides and phenolic constituents, and to investigate antioxidant, antimicrobial, anti-inflammatory, and cytotoxic activities of B. perfoliata methanolic extract. The secoiridoid glycosides swertiamarin, gentiopicrin, and sweroside were found to be the dominant compounds of the extract, while 23 phenolic compounds were identified in much lower concentrations. Among phenolics, flavanols were the most abundant, which represents a unique feature among Gentianaceae species. The extract showed moderate to weak antioxidant activity with better performance in inhibition of lipid peroxidation than in free radical scavenging activities. The extract showed generally better antifungal properties compared with its antibacterial potential. Also, B. perfoliata demonstrated in vitro anti-inflammatory activity, the extract (50 μg/mL) showed inhibition of cyclooxigenases, COX-1 and COX-2, activities (19.65 and 48.02%, respectively). It also displayed biocompatibility on the immortalized and cancer cells, as no cytotoxic effect was observed. For the first time, the bioactive potential of this species was demonstrated, justifying its usage in pharmaceutical and food products as an alternative for some overexploited and endangered species from the Gentianaceae family.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Blackstonia perfoliata (L.) Huds. (Gentianaceae): A promising source of useful bioactive compounds",
volume = "145",
doi = "10.1016/J.INDCROP.2019.111974",
pages = "111974"
}

Mihailović, V., Katanić Stanković, J. S., Jurić, T., Srećković, N., Mišić, D., Šiler, B., Monti, D. M., Imbimbo, P., Nikles, S., Pan, S.,& Bauer, R.. (2020). Blackstonia perfoliata (L.) Huds. (Gentianaceae): A promising source of useful bioactive compounds. in Industrial Crops and Products
Elsevier., 145, 111974.
https://doi.org/10.1016/J.INDCROP.2019.111974

Mihailović V, Katanić Stanković JS, Jurić T, Srećković N, Mišić D, Šiler B, Monti DM, Imbimbo P, Nikles S, Pan S, Bauer R. Blackstonia perfoliata (L.) Huds. (Gentianaceae): A promising source of useful bioactive compounds. in Industrial Crops and Products. 2020;145:111974.
doi:10.1016/J.INDCROP.2019.111974 .

Mihailović, Vladimir, Katanić Stanković, Jelena S., Jurić, Tatjana, Srećković, Nikola, Mišić, Danijela, Šiler, Branislav, Monti, Daria Maria, Imbimbo, Paola, Nikles, Stefanie, Pan, San-Po, Bauer, Rudolf, "Blackstonia perfoliata (L.) Huds. (Gentianaceae): A promising source of useful bioactive compounds" in Industrial Crops and Products, 145 (2020):111974,
https://doi.org/10.1016/J.INDCROP.2019.111974 . .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Paunović, Danijela
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3756
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4966
AB  - Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
PB  - Oxford University Press
T2  - Glycobiology
T1  - ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R
IS  - 1
IS  - 1
VL  - 30
VL  - 30
DO  - 10.1093/glycob/cwz072
SP  - 19
SP  - 19
EP  - 35
EP  - 35
ER  - 

@article{
author = "Dragićević, Milan and Paunović, Danijela and Bogdanović, Milica and Todorović, Slađana and Simonović, Ana",
year = "2020",
abstract = "Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.",
publisher = "Oxford University Press",
journal = "Glycobiology",
title = "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R",
number = "1, 1",
volume = "30, 30",
doi = "10.1093/glycob/cwz072",
pages = "19-19-35-35"
}

Dragićević, M., Paunović, D., Bogdanović, M., Todorović, S.,& Simonović, A.. (2020). ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology
Oxford University Press., 30(1), 19-35.
https://doi.org/10.1093/glycob/cwz072

Dragićević M, Paunović D, Bogdanović M, Todorović S, Simonović A. ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology. 2020;30(1):19-35.
doi:10.1093/glycob/cwz072 .

Dragićević, Milan, Paunović, Danijela, Bogdanović, Milica, Todorović, Slađana, Simonović, Ana, "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R" in Glycobiology, 30, no. 1 (2020):19-35,
https://doi.org/10.1093/glycob/cwz072 . .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Paunović, Danijela
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3756
AB  - Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
PB  - Oxford University Press
T2  - Glycobiology
T1  - ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R
IS  - 1
IS  - 1
VL  - 30
VL  - 30
DO  - 10.1093/glycob/cwz072
SP  - 19
SP  - 19
EP  - 35
EP  - 35
ER  - 

@article{
author = "Dragićević, Milan and Paunović, Danijela and Bogdanović, Milica and Todorović, Slađana and Simonović, Ana",
year = "2020",
abstract = "Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.",
publisher = "Oxford University Press",
journal = "Glycobiology",
title = "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R",
number = "1, 1",
volume = "30, 30",
doi = "10.1093/glycob/cwz072",
pages = "19-19-35-35"
}

Dragićević, M., Paunović, D., Bogdanović, M., Todorović, S.,& Simonović, A.. (2020). ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology
Oxford University Press., 30(1), 19-35.
https://doi.org/10.1093/glycob/cwz072

Dragićević M, Paunović D, Bogdanović M, Todorović S, Simonović A. ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology. 2020;30(1):19-35.
doi:10.1093/glycob/cwz072 .

Dragićević, Milan, Paunović, Danijela, Bogdanović, Milica, Todorović, Slađana, Simonović, Ana, "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R" in Glycobiology, 30, no. 1 (2020):19-35,
https://doi.org/10.1093/glycob/cwz072 . .

TY  - JOUR
AU  - Đurović, Saša
AU  - Vujanović, Milena
AU  - Radojković, Marija
AU  - Filipović, Jelena
AU  - Filipović, Vladimir
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Mašković, Pavle
AU  - Zeković, Zoran
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4067
AB  - Stinging nettle is an annual plant. This plant is known for applications in folk medicine, and as the human diet. The stinging nettle leaves has been used to obtain extracts, which are expected to be with a high content of biologically active compounds. Prepared microwave extracts were applied in the formulation of the functional products. The article completes preparation of the bread with addition of the stinging nettle leaves and its extract, and bread’s composition in phenolic acids, flavonoids, micro elements, and macro elements. The biological activity of the prepared bread samples showed significant antioxidant activity. This was especially true against DPPH radicals. It has been shown that it holds a high cytotoxic activity. Leaves themselves decreased the quality of the bread, while the extract improved the quality. It was sensorially confirmed. The article concluded that the extract substitutes leaves in bread as a product of a high benefit.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - The functional food production: Application of stinging nettle leaves and its extracts in the baking of a bread
VL  - 312
DO  - 10.1016/j.foodchem.2019.126091
SP  - 126091
ER  - 

@article{
author = "Đurović, Saša and Vujanović, Milena and Radojković, Marija and Filipović, Jelena and Filipović, Vladimir and Gašić, Uroš and Tešić, Živoslav and Mašković, Pavle and Zeković, Zoran",
year = "2020",
abstract = "Stinging nettle is an annual plant. This plant is known for applications in folk medicine, and as the human diet. The stinging nettle leaves has been used to obtain extracts, which are expected to be with a high content of biologically active compounds. Prepared microwave extracts were applied in the formulation of the functional products. The article completes preparation of the bread with addition of the stinging nettle leaves and its extract, and bread’s composition in phenolic acids, flavonoids, micro elements, and macro elements. The biological activity of the prepared bread samples showed significant antioxidant activity. This was especially true against DPPH radicals. It has been shown that it holds a high cytotoxic activity. Leaves themselves decreased the quality of the bread, while the extract improved the quality. It was sensorially confirmed. The article concluded that the extract substitutes leaves in bread as a product of a high benefit.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "The functional food production: Application of stinging nettle leaves and its extracts in the baking of a bread",
volume = "312",
doi = "10.1016/j.foodchem.2019.126091",
pages = "126091"
}

Đurović, S., Vujanović, M., Radojković, M., Filipović, J., Filipović, V., Gašić, U., Tešić, Ž., Mašković, P.,& Zeković, Z.. (2020). The functional food production: Application of stinging nettle leaves and its extracts in the baking of a bread. in Food Chemistry
Elsevier Ltd., 312, 126091.
https://doi.org/10.1016/j.foodchem.2019.126091

Đurović S, Vujanović M, Radojković M, Filipović J, Filipović V, Gašić U, Tešić Ž, Mašković P, Zeković Z. The functional food production: Application of stinging nettle leaves and its extracts in the baking of a bread. in Food Chemistry. 2020;312:126091.
doi:10.1016/j.foodchem.2019.126091 .

Đurović, Saša, Vujanović, Milena, Radojković, Marija, Filipović, Jelena, Filipović, Vladimir, Gašić, Uroš, Tešić, Živoslav, Mašković, Pavle, Zeković, Zoran, "The functional food production: Application of stinging nettle leaves and its extracts in the baking of a bread" in Food Chemistry, 312 (2020):126091,
https://doi.org/10.1016/j.foodchem.2019.126091 . .

TY  - JOUR
AU  - Stikić, Radmila I.
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Jovanović, Zorica B.
AU  - Gašić, Uroš
AU  - Tešić, Živoslav Lj.
AU  - Djordjević, Nataša Z.
AU  - Savić, Sladjana K.
AU  - Czekus, Borisz G.
AU  - Pešić, Mirjana
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/cche.10278
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3639
AB  - Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health‐promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. 13 phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration, and time‐dependent effects were more expressed in Puno extracts. The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT‐116, as well as the presence of 7 new phenolic and flavonoid compounds.
T2  - Cereal Chemistry
T1  - Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
IS  - 3
VL  - 97
DO  - 10.1002/cche.10278
SP  - cche.10278
SP  - 626
EP  - 633
ER  - 

@article{
author = "Stikić, Radmila I. and Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Jovanović, Zorica B. and Gašić, Uroš and Tešić, Živoslav Lj. and Djordjević, Nataša Z. and Savić, Sladjana K. and Czekus, Borisz G. and Pešić, Mirjana",
year = "2020",
abstract = "Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health‐promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. 13 phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration, and time‐dependent effects were more expressed in Puno extracts. The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT‐116, as well as the presence of 7 new phenolic and flavonoid compounds.",
journal = "Cereal Chemistry",
title = "Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars",
number = "3",
volume = "97",
doi = "10.1002/cche.10278",
pages = "cche.10278-626-633"
}

Stikić, R. I., Milinčić, D. D., Kostić, A. Ž., Jovanović, Z. B., Gašić, U., Tešić, Ž. Lj., Djordjević, N. Z., Savić, S. K., Czekus, B. G.,& Pešić, M.. (2020). Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry, 97(3), cche.10278-633.
https://doi.org/10.1002/cche.10278

Stikić RI, Milinčić DD, Kostić AŽ, Jovanović ZB, Gašić U, Tešić ŽL, Djordjević NZ, Savić SK, Czekus BG, Pešić M. Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry. 2020;97(3):cche.10278-633.
doi:10.1002/cche.10278 .

Stikić, Radmila I., Milinčić, Danijel D., Kostić, Aleksandar Ž., Jovanović, Zorica B., Gašić, Uroš, Tešić, Živoslav Lj., Djordjević, Nataša Z., Savić, Sladjana K., Czekus, Borisz G., Pešić, Mirjana, "Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars" in Cereal Chemistry, 97, no. 3 (2020):cche.10278-633,
https://doi.org/10.1002/cche.10278 . .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Cankar, Katarina
AU  - Dragićević, Milan
AU  - Bouwmeester, Harro
AU  - Beekwilder, Jules
AU  - Simonović, Ana
AU  - Todorović, Slađana
PY  - 2020
UR  - https://www.tandfonline.com/doi/full/10.1080/21645698.2019.1681868
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3520
AB  - Chicory (Cichorium intybus L.) is a medicinal and industrial plant from the Asteraceae family that produces a variety of sesquiterpene lactones (STLs), most importantly bitter guaianolides: lactucin, lactucopicrin and 8-deoxylactucin as well as their modified forms such as oxalates. These compounds have medicinal properties; however, they also hamper the extraction of inulin - a very important food industry product from chicory roots. The first step in guaianolide biosynthesis is catalyzed by germacrene A synthase (GAS) which in chicory exists in two isoforms - GAS long (encoded by CiGASlo) and GAS short (encoded by CiGASsh). AmiRNA silencing was used to obtain plants with reduced GAS gene expression and level of downstream metabolites, guaianolide-15-oxalates, as the major STLs in chicory. This approach could be beneficial for engineering new chicory varieties with varying STL content, and especially varieties with reduced bitter compounds more suitable for inulin production.
T2  - GM Crops & Food
T1  - Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.
IS  - 1
VL  - 11
DO  - 10.1080/21645698.2019.1681868
SP  - 54
EP  - 66
ER  - 

@article{
author = "Bogdanović, Milica and Cankar, Katarina and Dragićević, Milan and Bouwmeester, Harro and Beekwilder, Jules and Simonović, Ana and Todorović, Slađana",
year = "2020",
abstract = "Chicory (Cichorium intybus L.) is a medicinal and industrial plant from the Asteraceae family that produces a variety of sesquiterpene lactones (STLs), most importantly bitter guaianolides: lactucin, lactucopicrin and 8-deoxylactucin as well as their modified forms such as oxalates. These compounds have medicinal properties; however, they also hamper the extraction of inulin - a very important food industry product from chicory roots. The first step in guaianolide biosynthesis is catalyzed by germacrene A synthase (GAS) which in chicory exists in two isoforms - GAS long (encoded by CiGASlo) and GAS short (encoded by CiGASsh). AmiRNA silencing was used to obtain plants with reduced GAS gene expression and level of downstream metabolites, guaianolide-15-oxalates, as the major STLs in chicory. This approach could be beneficial for engineering new chicory varieties with varying STL content, and especially varieties with reduced bitter compounds more suitable for inulin production.",
journal = "GM Crops & Food",
title = "Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.",
number = "1",
volume = "11",
doi = "10.1080/21645698.2019.1681868",
pages = "54-66"
}

Bogdanović, M., Cankar, K., Dragićević, M., Bouwmeester, H., Beekwilder, J., Simonović, A.,& Todorović, S.. (2020). Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.. in GM Crops & Food, 11(1), 54-66.
https://doi.org/10.1080/21645698.2019.1681868

Bogdanović M, Cankar K, Dragićević M, Bouwmeester H, Beekwilder J, Simonović A, Todorović S. Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.. in GM Crops & Food. 2020;11(1):54-66.
doi:10.1080/21645698.2019.1681868 .

Bogdanović, Milica, Cankar, Katarina, Dragićević, Milan, Bouwmeester, Harro, Beekwilder, Jules, Simonović, Ana, Todorović, Slađana, "Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L." in GM Crops & Food, 11, no. 1 (2020):54-66,
https://doi.org/10.1080/21645698.2019.1681868 . .

TY  - JOUR
AU  - Jurić, Tatjana
AU  - Katanić Stanković, Jelena S.
AU  - Rosić, Gvozden
AU  - Selaković, Dragica
AU  - Joksimović, Jovana
AU  - Mišić, Danijela
AU  - Stanković, Vesna
AU  - Mihailović, Vladimir
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0254629919309962?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3522
AB  - This study was designed to evaluate the possible effect of methanol extracts of aerial parts and roots of Alchemilla vulgaris L. (AVA and AVR, respectively) in preventing cisplatin-induced hepatorenal and testicular toxicity in rats. UHPLC/DAD/(−)HESI-MS/MS analysis was performed to determine the detailed phenolic profile of AVA and AVR. The male Wistar rats were orally treated with extracts at three different concentrations (50, 100, and 200 mg/kg b.w.) for 10 days and toxicity was induced by injection of single dose of cisplatin on the 5th day (7.5 mg/kg b.w.). Determination of serum biochemical markers of hepatorenal and testicular tissue injury, as well as oxidative-stress parameters in tissues and histopathological study, were performed. Treatments with AVA and AVR significantly attenuated the levels of serum parameters of liver, kidneys and testicles injury, tissue's morphology and parameters of oxidative stress caused by an application of the cisplatin. Chromatographic analysis showed the presence of more than 20 different phenolic compounds in extracts where ellagic acid, catechin, and catechin gallate were dominant components in both extracts. The obtained results indicate that A. vulgaris extracts may be used in preventing cisplatin-induced toxicity during chemotherapy as well as in the treatment of oxidative stress-related disorders.
T2  - South African Journal of Botany
T1  - Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats
VL  - 128
DO  - 10.1016/J.SAJB.2019.09.010
SP  - 141
EP  - 151
ER  - 

@article{
author = "Jurić, Tatjana and Katanić Stanković, Jelena S. and Rosić, Gvozden and Selaković, Dragica and Joksimović, Jovana and Mišić, Danijela and Stanković, Vesna and Mihailović, Vladimir",
year = "2020",
abstract = "This study was designed to evaluate the possible effect of methanol extracts of aerial parts and roots of Alchemilla vulgaris L. (AVA and AVR, respectively) in preventing cisplatin-induced hepatorenal and testicular toxicity in rats. UHPLC/DAD/(−)HESI-MS/MS analysis was performed to determine the detailed phenolic profile of AVA and AVR. The male Wistar rats were orally treated with extracts at three different concentrations (50, 100, and 200 mg/kg b.w.) for 10 days and toxicity was induced by injection of single dose of cisplatin on the 5th day (7.5 mg/kg b.w.). Determination of serum biochemical markers of hepatorenal and testicular tissue injury, as well as oxidative-stress parameters in tissues and histopathological study, were performed. Treatments with AVA and AVR significantly attenuated the levels of serum parameters of liver, kidneys and testicles injury, tissue's morphology and parameters of oxidative stress caused by an application of the cisplatin. Chromatographic analysis showed the presence of more than 20 different phenolic compounds in extracts where ellagic acid, catechin, and catechin gallate were dominant components in both extracts. The obtained results indicate that A. vulgaris extracts may be used in preventing cisplatin-induced toxicity during chemotherapy as well as in the treatment of oxidative stress-related disorders.",
journal = "South African Journal of Botany",
title = "Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats",
volume = "128",
doi = "10.1016/J.SAJB.2019.09.010",
pages = "141-151"
}

Jurić, T., Katanić Stanković, J. S., Rosić, G., Selaković, D., Joksimović, J., Mišić, D., Stanković, V.,& Mihailović, V.. (2020). Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats. in South African Journal of Botany, 128, 141-151.
https://doi.org/10.1016/J.SAJB.2019.09.010

Jurić T, Katanić Stanković JS, Rosić G, Selaković D, Joksimović J, Mišić D, Stanković V, Mihailović V. Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats. in South African Journal of Botany. 2020;128:141-151.
doi:10.1016/J.SAJB.2019.09.010 .

Jurić, Tatjana, Katanić Stanković, Jelena S., Rosić, Gvozden, Selaković, Dragica, Joksimović, Jovana, Mišić, Danijela, Stanković, Vesna, Mihailović, Vladimir, "Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats" in South African Journal of Botany, 128 (2020):141-151,
https://doi.org/10.1016/J.SAJB.2019.09.010 . .

TY  - JOUR
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Maksimović, Vuk
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Božunović, Jelena
AU  - Banjanac, Tijana
AU  - Mišić, Danijela
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3699
AB  - Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH• and ABTS•+), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(–)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.
PB  - Elsevier B.V.
T2  - South African Journal of Botany
T1  - Antioxidant and antimicrobial activity of two Asplenium species
VL  - 132
DO  - 10.1016/j.sajb.2020.03.034
SP  - 180
EP  - 187
ER  - 

@article{
author = "Živković, Suzana and Milutinović, Milica and Maksimović, Vuk and Ćirić, Ana and Ivanov, Marija and Božunović, Jelena and Banjanac, Tijana and Mišić, Danijela",
year = "2020",
abstract = "Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH• and ABTS•+), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(–)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.",
publisher = "Elsevier B.V.",
journal = "South African Journal of Botany",
title = "Antioxidant and antimicrobial activity of two Asplenium species",
volume = "132",
doi = "10.1016/j.sajb.2020.03.034",
pages = "180-187"
}

Živković, S., Milutinović, M., Maksimović, V., Ćirić, A., Ivanov, M., Božunović, J., Banjanac, T.,& Mišić, D.. (2020). Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany
Elsevier B.V.., 132, 180-187.
https://doi.org/10.1016/j.sajb.2020.03.034

Živković S, Milutinović M, Maksimović V, Ćirić A, Ivanov M, Božunović J, Banjanac T, Mišić D. Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany. 2020;132:180-187.
doi:10.1016/j.sajb.2020.03.034 .

Živković, Suzana, Milutinović, Milica, Maksimović, Vuk, Ćirić, Ana, Ivanov, Marija, Božunović, Jelena, Banjanac, Tijana, Mišić, Danijela, "Antioxidant and antimicrobial activity of two Asplenium species" in South African Journal of Botany, 132 (2020):180-187,
https://doi.org/10.1016/j.sajb.2020.03.034 . .

TY  - THES
AU  - Božunović, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/123456789/3863
AB  - Centaurium erythraea Rafn (kičica) je bogat izvor farmakološki značajnih specijalizovanih metabolita, uključujući fenolne kiseline, flavonoide i, u najvećoj meri sekoiridoide. Sekoiridoidni glukozidi su monoterpenoidni molekuli čiji se skelet zasniva na ciklopentan-C-piranu za koji je, na poziciji C1 vezan molekul β-D glukoze. Ova jedinjenja podležu enzimatskoj hidrolizi u reakciji sa enzimom β-glukozidazom, pri čemu se oslobađaju odgovarajući aglikoni koji se dalje uključuju u metaboličke procese biljke. Biosinteza i razgradnja sekoiridoida kod kičice su koordinisani procesi koji obezbeđuju konstitutivno prisustvo ovih gorkih materija u tkivima biljke, a čija je primarna uloga odbrana od patogena i herbivora. Kao odgovor na stres povređivanjem u listovima kičice dolazi do intenzivnije akumulacije sekoiridoida, što je rezultat kako pojačane ekspresije većine biosintetskih gena, tako i snižene ekspresije -glukozidaze koja katalizuje prvi korak njihove razgradnje. Koordinisana ekspresija biosintetskih gena uključenih u metabolizam sekoiridoida regulisana je na transkripcionom nivou i nalazi se pod uticajem signalnog puta jasmonske kiseline. Identifikovane su dve varijante enzima -glukozidaza (CeBGLU1 i CeBGLU2) kod kičice koje pokazuju visoku specifičnost ka sekoiridoidnim glukozidima kao supstratu. Hidrolitička funkcija enzima potvrđena je njihovom heterologom ekspresijom u organizmu domaćinu (Escherichia coli), prečišćavanjem rekombinantnih proteina i enzimatskim testovima izvedenim u uslovima in vitro. Rezultati doktorske disertacije pružaju osnovu za primenu metaboličkog inženjeringa u cilju komercijalne i održive proizvodnje sekoiridoida iz obnovljivih izvora.
AB  - Centaurium erythraea Rafn (centaury) is a rich source of pharmaceutically active specialized metabolites including phenolic acids, flavonoids and especially secoiridoids. Secoiridoid glucosides are monoterpenoids based on a cyclopentan-C-pyran skeleton with the β-D glucose linked at C1 position. These compounds are subjected to hydrolysis catalyzed by enzymes β-glucosidases, and resulting aglycones could be subsequently directed towards different metabolic pathways in plants. Coordinated metabolic processes, such as biosynthesis and catabolism of secoiridoid glucosides, ensure constitutive presence of these bitter taste compounds in plant tissue, which play a decisive role in the defense against pathogens and herbivores. Pronounced accumulation of secoiridoids in centaury leaves observed during the response of plants to mechanical wounding was preceeded by the strong induction of the majority of biosynthetic genes as well as by the down-regulation of β-glucosidase, which catalyses the first step of secoiridoid glucosides catabolism. The expression of secoiridoids biosynthetic genes during response to wounding appears to be coordinately up-regulated and controlled at the transcriptional level, through the jasmonate signalling pathway. Two centaury β-glucosidases (CeBGLU1 and CeBGLU2), showing high substrate specificity for secoiridoid glucosides, were identified. Hydrolytic function of the two enzymes was confirmed by heterologous expression of candidate genes in the host organism (Escherichia coli), purification of recombinant proteins, and in vitro enzymatic assays. The results of the doctoral dissertation provide the basis for the further application of metabolic
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Uloga sekoiridoidnih glukozida i beta-glukozidaze u odbrambenom odgovoru kičice (Centaurium erythraea Rafn) na stres povređivanjem
T1  - The role of secoiridoid glucosides and beta-glucosidase in common centaury (Centaurium erythraea Rafn) defense response to wounding
SP  - 1
EP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3863
ER  - 

@phdthesis{
author = "Božunović, Jelena",
year = "2020",
abstract = "Centaurium erythraea Rafn (kičica) je bogat izvor farmakološki značajnih specijalizovanih metabolita, uključujući fenolne kiseline, flavonoide i, u najvećoj meri sekoiridoide. Sekoiridoidni glukozidi su monoterpenoidni molekuli čiji se skelet zasniva na ciklopentan-C-piranu za koji je, na poziciji C1 vezan molekul β-D glukoze. Ova jedinjenja podležu enzimatskoj hidrolizi u reakciji sa enzimom β-glukozidazom, pri čemu se oslobađaju odgovarajući aglikoni koji se dalje uključuju u metaboličke procese biljke. Biosinteza i razgradnja sekoiridoida kod kičice su koordinisani procesi koji obezbeđuju konstitutivno prisustvo ovih gorkih materija u tkivima biljke, a čija je primarna uloga odbrana od patogena i herbivora. Kao odgovor na stres povređivanjem u listovima kičice dolazi do intenzivnije akumulacije sekoiridoida, što je rezultat kako pojačane ekspresije većine biosintetskih gena, tako i snižene ekspresije -glukozidaze koja katalizuje prvi korak njihove razgradnje. Koordinisana ekspresija biosintetskih gena uključenih u metabolizam sekoiridoida regulisana je na transkripcionom nivou i nalazi se pod uticajem signalnog puta jasmonske kiseline. Identifikovane su dve varijante enzima -glukozidaza (CeBGLU1 i CeBGLU2) kod kičice koje pokazuju visoku specifičnost ka sekoiridoidnim glukozidima kao supstratu. Hidrolitička funkcija enzima potvrđena je njihovom heterologom ekspresijom u organizmu domaćinu (Escherichia coli), prečišćavanjem rekombinantnih proteina i enzimatskim testovima izvedenim u uslovima in vitro. Rezultati doktorske disertacije pružaju osnovu za primenu metaboličkog inženjeringa u cilju komercijalne i održive proizvodnje sekoiridoida iz obnovljivih izvora., Centaurium erythraea Rafn (centaury) is a rich source of pharmaceutically active specialized metabolites including phenolic acids, flavonoids and especially secoiridoids. Secoiridoid glucosides are monoterpenoids based on a cyclopentan-C-pyran skeleton with the β-D glucose linked at C1 position. These compounds are subjected to hydrolysis catalyzed by enzymes β-glucosidases, and resulting aglycones could be subsequently directed towards different metabolic pathways in plants. Coordinated metabolic processes, such as biosynthesis and catabolism of secoiridoid glucosides, ensure constitutive presence of these bitter taste compounds in plant tissue, which play a decisive role in the defense against pathogens and herbivores. Pronounced accumulation of secoiridoids in centaury leaves observed during the response of plants to mechanical wounding was preceeded by the strong induction of the majority of biosynthetic genes as well as by the down-regulation of β-glucosidase, which catalyses the first step of secoiridoid glucosides catabolism. The expression of secoiridoids biosynthetic genes during response to wounding appears to be coordinately up-regulated and controlled at the transcriptional level, through the jasmonate signalling pathway. Two centaury β-glucosidases (CeBGLU1 and CeBGLU2), showing high substrate specificity for secoiridoid glucosides, were identified. Hydrolytic function of the two enzymes was confirmed by heterologous expression of candidate genes in the host organism (Escherichia coli), purification of recombinant proteins, and in vitro enzymatic assays. The results of the doctoral dissertation provide the basis for the further application of metabolic",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Uloga sekoiridoidnih glukozida i beta-glukozidaze u odbrambenom odgovoru kičice (Centaurium erythraea Rafn) na stres povređivanjem, The role of secoiridoid glucosides and beta-glucosidase in common centaury (Centaurium erythraea Rafn) defense response to wounding",
pages = "1-133",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3863"
}

Božunović, J.. (2020). Uloga sekoiridoidnih glukozida i beta-glukozidaze u odbrambenom odgovoru kičice (Centaurium erythraea Rafn) na stres povređivanjem. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-133.
https://hdl.handle.net/21.15107/rcub_ibiss_3863

Božunović J. Uloga sekoiridoidnih glukozida i beta-glukozidaze u odbrambenom odgovoru kičice (Centaurium erythraea Rafn) na stres povređivanjem. in University of Belgrade, Faculty of Biology. 2020;:1-133.
https://hdl.handle.net/21.15107/rcub_ibiss_3863 .

Božunović, Jelena, "Uloga sekoiridoidnih glukozida i beta-glukozidaze u odbrambenom odgovoru kičice (Centaurium erythraea Rafn) na stres povređivanjem" in University of Belgrade, Faculty of Biology (2020):1-133,
https://hdl.handle.net/21.15107/rcub_ibiss_3863 .

TY  - JOUR
AU  - Ćuković, Katarina
AU  - Dragićević, Milan
AU  - Bogdanović, Milica
AU  - Paunović, Danijela
AU  - Giurato, Giorgio
AU  - Filipović, Biljana
AU  - Subotić, Angelina
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3686
AB  - Centaurium erythraea Rafn. (common centaury, Gentianaceae) is a medicinal plant with great regeneration potential and developmental plasticity in vitro. Centaury can be regenerated from leaf explants by both somatic embryogenesis (SE) and shoot development (SD). We believe that its regeneration potential and developmental plasticity rest on high activity of certain genes, which may not be active or present in species recalcitrant to in vitro regeneration. However, there are no sequenced Gentianaceae genomes to support investigation of the molecular events during SE or SD. To this end, we have sequenced six centaury transcriptomes (embryogenic calli, globular somatic embryos, cotyledonary somatic embryos, adventitious buds, leaves and roots of in vitro grown plants) and de novo assembled centaury referent transcriptome comprising 105.726 genes. The high quality and completeness transcriptome was functionally annotated against NCBI nt, Swissprot and PFAM databases with KOG and GO enrichment. In addition, 11 housekeeping and functional genes were validated for expression stability in 27 tissue samples representing the processes of SE and SD, plants from nature and wounded tissues using GeNorm and NormFinder. The most stable genes that can be used for expression studies during SE, SD and in vitro manipulations are Ribosomal protein L2 (RPL2) and TATA binding protein 1 (TBP1) in combination with RAS (Rat Sarcoma)-related Nuclear protein (RAN) or Adenosine kinase (AK). These results comprise a complete framework for the search for genes involved in SE and SD, but may also be useful in identifying genes involved in biosynthesis of C. erythraea secondary metabolites.
PB  - Springer
T2  - Plant Cell, Tissue and Organ Culture
T1  - Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis
IS  - 2
VL  - 141
DO  - 10.1007/s11240-020-01801-w
SP  - 417
EP  - 433
ER  - 

@article{
author = "Ćuković, Katarina and Dragićević, Milan and Bogdanović, Milica and Paunović, Danijela and Giurato, Giorgio and Filipović, Biljana and Subotić, Angelina and Todorović, Slađana and Simonović, Ana",
year = "2020",
abstract = "Centaurium erythraea Rafn. (common centaury, Gentianaceae) is a medicinal plant with great regeneration potential and developmental plasticity in vitro. Centaury can be regenerated from leaf explants by both somatic embryogenesis (SE) and shoot development (SD). We believe that its regeneration potential and developmental plasticity rest on high activity of certain genes, which may not be active or present in species recalcitrant to in vitro regeneration. However, there are no sequenced Gentianaceae genomes to support investigation of the molecular events during SE or SD. To this end, we have sequenced six centaury transcriptomes (embryogenic calli, globular somatic embryos, cotyledonary somatic embryos, adventitious buds, leaves and roots of in vitro grown plants) and de novo assembled centaury referent transcriptome comprising 105.726 genes. The high quality and completeness transcriptome was functionally annotated against NCBI nt, Swissprot and PFAM databases with KOG and GO enrichment. In addition, 11 housekeeping and functional genes were validated for expression stability in 27 tissue samples representing the processes of SE and SD, plants from nature and wounded tissues using GeNorm and NormFinder. The most stable genes that can be used for expression studies during SE, SD and in vitro manipulations are Ribosomal protein L2 (RPL2) and TATA binding protein 1 (TBP1) in combination with RAS (Rat Sarcoma)-related Nuclear protein (RAN) or Adenosine kinase (AK). These results comprise a complete framework for the search for genes involved in SE and SD, but may also be useful in identifying genes involved in biosynthesis of C. erythraea secondary metabolites.",
publisher = "Springer",
journal = "Plant Cell, Tissue and Organ Culture",
title = "Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis",
number = "2",
volume = "141",
doi = "10.1007/s11240-020-01801-w",
pages = "417-433"
}

Ćuković, K., Dragićević, M., Bogdanović, M., Paunović, D., Giurato, G., Filipović, B., Subotić, A., Todorović, S.,& Simonović, A.. (2020). Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis. in Plant Cell, Tissue and Organ Culture
Springer., 141(2), 417-433.
https://doi.org/10.1007/s11240-020-01801-w

Ćuković K, Dragićević M, Bogdanović M, Paunović D, Giurato G, Filipović B, Subotić A, Todorović S, Simonović A. Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis. in Plant Cell, Tissue and Organ Culture. 2020;141(2):417-433.
doi:10.1007/s11240-020-01801-w .

Ćuković, Katarina, Dragićević, Milan, Bogdanović, Milica, Paunović, Danijela, Giurato, Giorgio, Filipović, Biljana, Subotić, Angelina, Todorović, Slađana, Simonović, Ana, "Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis" in Plant Cell, Tissue and Organ Culture, 141, no. 2 (2020):417-433,
https://doi.org/10.1007/s11240-020-01801-w . .

TY  - JOUR
AU  - Šuković, Danijela
AU  - Knežević, Bojana
AU  - Gašić, Uroš
AU  - Sredojević, Milica
AU  - Ćirić, Ivanka
AU  - Todić, Slavica
AU  - Mutić, Jelena
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://www.mdpi.com/2304-8158/9/2/138
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3607
AB  - Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.
T2  - Foods (Basel, Switzerland)
T1  - Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.
IS  - 2
VL  - 9
DO  - 10.3390/foods9020138
SP  - 138
ER  - 

@article{
author = "Šuković, Danijela and Knežević, Bojana and Gašić, Uroš and Sredojević, Milica and Ćirić, Ivanka and Todić, Slavica and Mutić, Jelena and Tešić, Živoslav",
year = "2020",
abstract = "Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.",
journal = "Foods (Basel, Switzerland)",
title = "Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.",
number = "2",
volume = "9",
doi = "10.3390/foods9020138",
pages = "138"
}

Šuković, D., Knežević, B., Gašić, U., Sredojević, M., Ćirić, I., Todić, S., Mutić, J.,& Tešić, Ž.. (2020). Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.. in Foods (Basel, Switzerland), 9(2), 138.
https://doi.org/10.3390/foods9020138

Šuković D, Knežević B, Gašić U, Sredojević M, Ćirić I, Todić S, Mutić J, Tešić Ž. Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.. in Foods (Basel, Switzerland). 2020;9(2):138.
doi:10.3390/foods9020138 .

Šuković, Danijela, Knežević, Bojana, Gašić, Uroš, Sredojević, Milica, Ćirić, Ivanka, Todić, Slavica, Mutić, Jelena, Tešić, Živoslav, "Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro." in Foods (Basel, Switzerland), 9, no. 2 (2020):138,
https://doi.org/10.3390/foods9020138 . .

TY  - JOUR
AU  - Katanić Stanković, Jelena S.
AU  - Srećković, Nikola
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Imbimbo, Paola
AU  - Monti, Daria Maria
AU  - Mihailović, Vladimir
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0926669019309422?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3523
AB  - The plants from genus Salvia, as one of the largest genus in Lamiaceae family, are frequently in use for various purposes, as foods, in cosmetic industry, or in traditional and official medicine. Salvia verticillata L. (liliac sage) is one of sidelined sage species with potential bioactivity, reported in traditional medicine. The aim of this study was to acquire a phytochemical profile of the methanol extract obtained from S. verticillata aerial parts and to evaluate its antioxidant, antimicrobial, and biocompatibility potential. Characteristic compounds of the genus Salvia, such as rosmarinic and caffeic acids, along with their derivatives (e.g. salvianolic and yunnaneic acids isomers) and flavonoids, have been identified by ultrahigh-performance Orbitrap metabolomic fingerprinting as the main phenolic metabolites in S. verticillata. The extract displayed moderate antimicrobial properties and significant antioxidant potential, with the half maximal inhibitory concentration values (IC50) ranging from 33 to 73 μg/mL. Importantly, full biocompatibility of the extract with eukaryotic cell lines was observed up to 72 h. The obtained results revealed the presence of polyphenolic bioactive compounds in S. verticillata extract with promising antioxidant potential and significant biocompatibility. In this regard, S. verticillata can find new perspectives of application as a food ingredient, in cosmetic and pharmaceutical industries, as it represents a valuable source of compounds with prominent health properties, with a special focus on rosmarinic acid.
T2  - Industrial Crops and Products
T1  - Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid
VL  - 143
DO  - 10.1016/J.INDCROP.2019.111932
SP  - 111932
ER  - 

@article{
author = "Katanić Stanković, Jelena S. and Srećković, Nikola and Mišić, Danijela and Gašić, Uroš and Imbimbo, Paola and Monti, Daria Maria and Mihailović, Vladimir",
year = "2020",
abstract = "The plants from genus Salvia, as one of the largest genus in Lamiaceae family, are frequently in use for various purposes, as foods, in cosmetic industry, or in traditional and official medicine. Salvia verticillata L. (liliac sage) is one of sidelined sage species with potential bioactivity, reported in traditional medicine. The aim of this study was to acquire a phytochemical profile of the methanol extract obtained from S. verticillata aerial parts and to evaluate its antioxidant, antimicrobial, and biocompatibility potential. Characteristic compounds of the genus Salvia, such as rosmarinic and caffeic acids, along with their derivatives (e.g. salvianolic and yunnaneic acids isomers) and flavonoids, have been identified by ultrahigh-performance Orbitrap metabolomic fingerprinting as the main phenolic metabolites in S. verticillata. The extract displayed moderate antimicrobial properties and significant antioxidant potential, with the half maximal inhibitory concentration values (IC50) ranging from 33 to 73 μg/mL. Importantly, full biocompatibility of the extract with eukaryotic cell lines was observed up to 72 h. The obtained results revealed the presence of polyphenolic bioactive compounds in S. verticillata extract with promising antioxidant potential and significant biocompatibility. In this regard, S. verticillata can find new perspectives of application as a food ingredient, in cosmetic and pharmaceutical industries, as it represents a valuable source of compounds with prominent health properties, with a special focus on rosmarinic acid.",
journal = "Industrial Crops and Products",
title = "Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid",
volume = "143",
doi = "10.1016/J.INDCROP.2019.111932",
pages = "111932"
}

Katanić Stanković, J. S., Srećković, N., Mišić, D., Gašić, U., Imbimbo, P., Monti, D. M.,& Mihailović, V.. (2020). Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid. in Industrial Crops and Products, 143, 111932.
https://doi.org/10.1016/J.INDCROP.2019.111932

Katanić Stanković JS, Srećković N, Mišić D, Gašić U, Imbimbo P, Monti DM, Mihailović V. Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid. in Industrial Crops and Products. 2020;143:111932.
doi:10.1016/J.INDCROP.2019.111932 .

Katanić Stanković, Jelena S., Srećković, Nikola, Mišić, Danijela, Gašić, Uroš, Imbimbo, Paola, Monti, Daria Maria, Mihailović, Vladimir, "Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid" in Industrial Crops and Products, 143 (2020):111932,
https://doi.org/10.1016/J.INDCROP.2019.111932 . .

TY  - JOUR
AU  - Božunović, Jelena
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Živković, Suzana
AU  - Dragićević, Milan
AU  - Aničić, Neda
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Mišić, Danijela
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4346
AB  - Centaurium erythraea Rafn produces and accumulates various biologically active specialized metabolites, including secoiridoid glucosides (SGs), which help plants to cope with unfavorable environmental conditions. Specialized metabolism is commonly modulated in a way to increase the level of protective metabolites, such as SGs. Here, we report the molecular background of the wounding-induced changes in SGs metabolism for the first time. The mechanical wounding of leaves leads to a coordinated up-regulation of SGs biosynthetic genes and corresponding JA-related transcription factors (TFs) after 24 h, which results in the increase of metabolic flux through the biosynthetic pathway and, finally, leads to the elevated accumulation of SGs 96 h upon injury. The most pronounced increase in relative expression was detected for secologanin synthase (CeSLS), highlighting this enzyme as an important point for the regulation of biosynthetic flux through the SG pathway. A similar expression pattern was observed for CeBIS1, imposing itself as the TF that is prominently involved in wound-induced regulation of SGs biosynthesis genes. The high degree of positive correlations between and among the biosynthetic genes and targeted TFs expressions indicate the transcriptional regulation of SGs biosynthesis in response to wounding with a significant role of CeBIS1, which is a known component of the jasmonic acid (JA) signaling pathway. View Full-Text
PB  - Basel: MDPI
T2  - Plants
T1  - Secoiridoids Metabolism Response to Wounding in Common Centaury (Centaurium erythraea Rafn) Leaves
IS  - 12
VL  - 8
DO  - 10.3390/plants8120589
SP  - 589
ER  - 

@article{
author = "Božunović, Jelena and Skorić, Marijana and Matekalo, Dragana and Živković, Suzana and Dragićević, Milan and Aničić, Neda and Filipović, Biljana and Banjanac, Tijana and Šiler, Branislav and Mišić, Danijela",
year = "2019",
abstract = "Centaurium erythraea Rafn produces and accumulates various biologically active specialized metabolites, including secoiridoid glucosides (SGs), which help plants to cope with unfavorable environmental conditions. Specialized metabolism is commonly modulated in a way to increase the level of protective metabolites, such as SGs. Here, we report the molecular background of the wounding-induced changes in SGs metabolism for the first time. The mechanical wounding of leaves leads to a coordinated up-regulation of SGs biosynthetic genes and corresponding JA-related transcription factors (TFs) after 24 h, which results in the increase of metabolic flux through the biosynthetic pathway and, finally, leads to the elevated accumulation of SGs 96 h upon injury. The most pronounced increase in relative expression was detected for secologanin synthase (CeSLS), highlighting this enzyme as an important point for the regulation of biosynthetic flux through the SG pathway. A similar expression pattern was observed for CeBIS1, imposing itself as the TF that is prominently involved in wound-induced regulation of SGs biosynthesis genes. The high degree of positive correlations between and among the biosynthetic genes and targeted TFs expressions indicate the transcriptional regulation of SGs biosynthesis in response to wounding with a significant role of CeBIS1, which is a known component of the jasmonic acid (JA) signaling pathway. View Full-Text",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Secoiridoids Metabolism Response to Wounding in Common Centaury (Centaurium erythraea Rafn) Leaves",
number = "12",
volume = "8",
doi = "10.3390/plants8120589",
pages = "589"
}

Božunović, J., Skorić, M., Matekalo, D., Živković, S., Dragićević, M., Aničić, N., Filipović, B., Banjanac, T., Šiler, B.,& Mišić, D.. (2019). Secoiridoids Metabolism Response to Wounding in Common Centaury (Centaurium erythraea Rafn) Leaves. in Plants
Basel: MDPI., 8(12), 589.
https://doi.org/10.3390/plants8120589

Božunović J, Skorić M, Matekalo D, Živković S, Dragićević M, Aničić N, Filipović B, Banjanac T, Šiler B, Mišić D. Secoiridoids Metabolism Response to Wounding in Common Centaury (Centaurium erythraea Rafn) Leaves. in Plants. 2019;8(12):589.
doi:10.3390/plants8120589 .

Božunović, Jelena, Skorić, Marijana, Matekalo, Dragana, Živković, Suzana, Dragićević, Milan, Aničić, Neda, Filipović, Biljana, Banjanac, Tijana, Šiler, Branislav, Mišić, Danijela, "Secoiridoids Metabolism Response to Wounding in Common Centaury (Centaurium erythraea Rafn) Leaves" in Plants, 8, no. 12 (2019):589,
https://doi.org/10.3390/plants8120589 . .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragićević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Mišić, Danijela
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161719300525?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3342
AB  - Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.
T2  - Journal of Plant Physiology
T1  - Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.
VL  - 237
DO  - 10.1016/j.jplph.2019.04.006
SP  - 87
EP  - 94
ER  - 

@article{
author = "Dmitrović, Slavica and Dragićević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Mišić, Danijela",
year = "2019",
abstract = "Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.",
journal = "Journal of Plant Physiology",
title = "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.",
volume = "237",
doi = "10.1016/j.jplph.2019.04.006",
pages = "87-94"
}

Dmitrović, S., Dragićević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D.,& Mišić, D.. (2019). Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology, 237, 87-94.
https://doi.org/10.1016/j.jplph.2019.04.006

Dmitrović S, Dragićević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Mišić D. Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology. 2019;237:87-94.
doi:10.1016/j.jplph.2019.04.006 .

Dmitrović, Slavica, Dragićević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Mišić, Danijela, "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh." in Journal of Plant Physiology, 237 (2019):87-94,
https://doi.org/10.1016/j.jplph.2019.04.006 . .