TY  - CONF
AU  - Roljić, Rajko
AU  - Atanacković, Ana
AU  - Nikolić, Vera
AU  - Marković, Vanja
AU  - Zorić, Katarina
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7029
AB  - Branchiobdellidans are clitellate annelids that occur mainly on astacoidean crayfishes as obligate epibionts. In Europe, all native species belong to the genus Branchiobdella Odier, 1823, which is represented by nine species. Of these, five species have been recorded in the Western Balkans Branchiobdella astaci Odier, 1823, B. parasita (Braun, 1805), B. pentadonta Whitman, 1882, B. hexadonta Grüber, 1883, B. italica Canegallo, 1928, and B. balcanica Moszynsky, 1938. The aim of the work is to improve our knowledge of the occurrence of these species in the Balkan region. Samples of Austropotamobius torrentium were collected from three streams: Miljevka, Govza and Bistrica, belonging to the River Drina basin in the municipality of Foča in south-eastern Bosnia and Herzegovina. Based on the morphological characteristics of the jaw - shape and dentation, the following species were identified: Branchiobdella parasita and species from the pentadonta complex. Since the two species of the pentadonta complex, B. italica and B. pentadonta, are morphologically very similar and both were previously found on A. torrentium, an exact identification of the species is possible using molecular-phylogenetic analyses that are planned in upcoming period. The obtained results are consistent with the literature data. However, if confirmed, the finding of B. italica on stone crayfish from the Black Sea basin would be of particular importance. Until now, the distribution of B. italica was restricted only to the watercourses of the Adriatic Sea basin.
PB  - Zagreb: Croatian Ecological Society
C3  - Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia
T1  - A contribution to the knowledge of the Branchiobdella species of the Balkan region
SP  - 125
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_7029
ER  - 

@conference{
author = "Roljić, Rajko and Atanacković, Ana and Nikolić, Vera and Marković, Vanja and Zorić, Katarina",
year = "2024",
abstract = "Branchiobdellidans are clitellate annelids that occur mainly on astacoidean crayfishes as obligate epibionts. In Europe, all native species belong to the genus Branchiobdella Odier, 1823, which is represented by nine species. Of these, five species have been recorded in the Western Balkans Branchiobdella astaci Odier, 1823, B. parasita (Braun, 1805), B. pentadonta Whitman, 1882, B. hexadonta Grüber, 1883, B. italica Canegallo, 1928, and B. balcanica Moszynsky, 1938. The aim of the work is to improve our knowledge of the occurrence of these species in the Balkan region. Samples of Austropotamobius torrentium were collected from three streams: Miljevka, Govza and Bistrica, belonging to the River Drina basin in the municipality of Foča in south-eastern Bosnia and Herzegovina. Based on the morphological characteristics of the jaw - shape and dentation, the following species were identified: Branchiobdella parasita and species from the pentadonta complex. Since the two species of the pentadonta complex, B. italica and B. pentadonta, are morphologically very similar and both were previously found on A. torrentium, an exact identification of the species is possible using molecular-phylogenetic analyses that are planned in upcoming period. The obtained results are consistent with the literature data. However, if confirmed, the finding of B. italica on stone crayfish from the Black Sea basin would be of particular importance. Until now, the distribution of B. italica was restricted only to the watercourses of the Adriatic Sea basin.",
publisher = "Zagreb: Croatian Ecological Society",
journal = "Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia",
title = "A contribution to the knowledge of the Branchiobdella species of the Balkan region",
pages = "125",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7029"
}

Roljić, R., Atanacković, A., Nikolić, V., Marković, V.,& Zorić, K.. (2024). A contribution to the knowledge of the Branchiobdella species of the Balkan region. in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia
Zagreb: Croatian Ecological Society., 125.
https://hdl.handle.net/21.15107/rcub_ibiss_7029

Roljić R, Atanacković A, Nikolić V, Marković V, Zorić K. A contribution to the knowledge of the Branchiobdella species of the Balkan region. in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia. 2024;:125.
https://hdl.handle.net/21.15107/rcub_ibiss_7029 .

Roljić, Rajko, Atanacković, Ana, Nikolić, Vera, Marković, Vanja, Zorić, Katarina, "A contribution to the knowledge of the Branchiobdella species of the Balkan region" in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia (2024):125,
https://hdl.handle.net/21.15107/rcub_ibiss_7029 .

TY  - CONF
AU  - Roljić, Rajko
AU  - Nikolić, Vera
AU  - Zorić, Katarina
AU  - Marković, Vanja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7031
AB  - The non-native species Faxonius limosus belongs to the family Cambaridae and is the first invasive crayfish species to be introduced into Europe. In recent decades, it has been observed in the entire part of the Danube River in Serbia, as well as in the Sava, Tisa, Velika Morava rivers and in the Ćelije Reservoir. The study included 111 specimens (48 from the Danube River and 63 from the Ćelije Reservoir). The analysis includes measurement of 21 morphometric parameters per crayfish, and fitness indices were also calculated. The results partially fit within the known range of variation and represent preliminary data for both habitats. Results showed significant differences between males inhabiting different habitats, while females differed mainly in measurements of cephalothorax. Males were in better body condition compared to females, while both sexes in the Danube River had higher fitness indices compared to the Ćelija Reservoir population. We can assume that the observed morphometric differences are the result of crayfish adaptation to the specific ecological conditions in the different habitats.
PB  - Zagreb: Croatian Ecological Society
C3  - Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia
T1  - Morphological variability and condition of introduced spiny-cheek crayfish populations occupying different habitats in Serbia
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_7031
ER  - 

@conference{
author = "Roljić, Rajko and Nikolić, Vera and Zorić, Katarina and Marković, Vanja",
year = "2024",
abstract = "The non-native species Faxonius limosus belongs to the family Cambaridae and is the first invasive crayfish species to be introduced into Europe. In recent decades, it has been observed in the entire part of the Danube River in Serbia, as well as in the Sava, Tisa, Velika Morava rivers and in the Ćelije Reservoir. The study included 111 specimens (48 from the Danube River and 63 from the Ćelije Reservoir). The analysis includes measurement of 21 morphometric parameters per crayfish, and fitness indices were also calculated. The results partially fit within the known range of variation and represent preliminary data for both habitats. Results showed significant differences between males inhabiting different habitats, while females differed mainly in measurements of cephalothorax. Males were in better body condition compared to females, while both sexes in the Danube River had higher fitness indices compared to the Ćelija Reservoir population. We can assume that the observed morphometric differences are the result of crayfish adaptation to the specific ecological conditions in the different habitats.",
publisher = "Zagreb: Croatian Ecological Society",
journal = "Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia",
title = "Morphological variability and condition of introduced spiny-cheek crayfish populations occupying different habitats in Serbia",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7031"
}

Roljić, R., Nikolić, V., Zorić, K.,& Marković, V.. (2024). Morphological variability and condition of introduced spiny-cheek crayfish populations occupying different habitats in Serbia. in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia
Zagreb: Croatian Ecological Society., 124.
https://hdl.handle.net/21.15107/rcub_ibiss_7031

Roljić R, Nikolić V, Zorić K, Marković V. Morphological variability and condition of introduced spiny-cheek crayfish populations occupying different habitats in Serbia. in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia. 2024;:124.
https://hdl.handle.net/21.15107/rcub_ibiss_7031 .

Roljić, Rajko, Nikolić, Vera, Zorić, Katarina, Marković, Vanja, "Morphological variability and condition of introduced spiny-cheek crayfish populations occupying different habitats in Serbia" in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia (2024):124,
https://hdl.handle.net/21.15107/rcub_ibiss_7031 .

TY  - CONF
AU  - Lupšić, Ema
AU  - Dinić, Jelena
AU  - Dragoj, Miodrag
AU  - Jovanović Stojanov, Sofija
AU  - Stepanović, Ana
AU  - Pajović, Milica
AU  - Mohr, Thomas
AU  - Glumac, Sofija
AU  - Marić, Dragana
AU  - Ercegovac, Maja
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7032
AB  - Lung cancer remains the leading cause of cancer death globally, with non-small cell lung cancer (NSCLC) accounting for the majority of cases. Despite advances in diagnosis and treatment modalities, NSCLC remains a major challenge in clinical oncology. Recently, a functional diagnostics approach has been proposed, integrating the treatment response of patient-derived cancer cells with the patient’s genetic profile to recommend the optimal therapy for NSCLC. The aim of our study was to evaluate the response of 17 patient-derived NSCLC cultures to 10 commonly prescribed tyrosine kinase inhibitors (TKIs) and correlate these responses with patient mutational profiles. 
Primary patient-derived cultures, established from the NSCLC resections, with a mixed population of cancer and non-cancer cells were treated with 10 TKIs (afatinib, alectinib, ceritinib, crizotinib, dabrafenib, erlotinib, gefitinib, nintedanib, osimertinib and trametinib). Immunofluorescence assay enabling discrimination of epithelial cancer cells positive to a cocktail of antibodies against cytokeratin 8/18 vs. negative mesenchymal non-cancer cells was conducted using high-content imager ImageXpress Pico (Molecular Devices) with CellReporterXpress 2.9 software. As a part of a functional diagnostic approach whole-exome sequencing (WES) was performed to provide a comprehensive molecular profile of the patient's tumor.
NSCLC cultures responded differently to TKIs, with erlotinib showing good efficacy regardless of mutation burden or EGFR status. In addition, erlotinib exclusively inhibited the growth of cancer cells in NSCLC cultures while sparing stromal cells (CK8/18- cells). Other TKIs showed limited efficacy, highlighting the variability of response in NSCLC. Genetic alterations in signaling pathways associated with drug resistance and sensitivity, likely contributed to the variable responses to TKIs. Notably, TP53 was the most frequently mutated gene, with alterations detected in 65% of patients. In addition, the results from the KEGG pathways analysis showed that the variants were highly clustered in several signaling pathways such as PI3K-Akt pathway, EGFR tyrosine kinase inhibitor resistance and ErbB pathway.
Our results suggest that in addition to mutational status, performing functional sensitivity screening is critical for identifying appropriate treatment strategies with TKIs. These results underscore the importance of considering drug sensitivity, off-target effects, and patient-specific genetic profiles to optimize treatment strategies for NSCLC.
PB  - EMBO press
C3  - Abstract book: Molecular mechanisms in signal transduction and cancer: EMBO-FEBS Lecture course; 2024 Aug 18-26; Spetses, Greece
T1  - Functional diagnostics in non-small cell lung carcinoma - the potential for personalized approach
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_7032
ER  - 

@conference{
author = "Lupšić, Ema and Dinić, Jelena and Dragoj, Miodrag and Jovanović Stojanov, Sofija and Stepanović, Ana and Pajović, Milica and Mohr, Thomas and Glumac, Sofija and Marić, Dragana and Ercegovac, Maja and Podolski-Renić, Ana and Pešić, Milica",
year = "2024",
abstract = "Lung cancer remains the leading cause of cancer death globally, with non-small cell lung cancer (NSCLC) accounting for the majority of cases. Despite advances in diagnosis and treatment modalities, NSCLC remains a major challenge in clinical oncology. Recently, a functional diagnostics approach has been proposed, integrating the treatment response of patient-derived cancer cells with the patient’s genetic profile to recommend the optimal therapy for NSCLC. The aim of our study was to evaluate the response of 17 patient-derived NSCLC cultures to 10 commonly prescribed tyrosine kinase inhibitors (TKIs) and correlate these responses with patient mutational profiles. 
Primary patient-derived cultures, established from the NSCLC resections, with a mixed population of cancer and non-cancer cells were treated with 10 TKIs (afatinib, alectinib, ceritinib, crizotinib, dabrafenib, erlotinib, gefitinib, nintedanib, osimertinib and trametinib). Immunofluorescence assay enabling discrimination of epithelial cancer cells positive to a cocktail of antibodies against cytokeratin 8/18 vs. negative mesenchymal non-cancer cells was conducted using high-content imager ImageXpress Pico (Molecular Devices) with CellReporterXpress 2.9 software. As a part of a functional diagnostic approach whole-exome sequencing (WES) was performed to provide a comprehensive molecular profile of the patient's tumor.
NSCLC cultures responded differently to TKIs, with erlotinib showing good efficacy regardless of mutation burden or EGFR status. In addition, erlotinib exclusively inhibited the growth of cancer cells in NSCLC cultures while sparing stromal cells (CK8/18- cells). Other TKIs showed limited efficacy, highlighting the variability of response in NSCLC. Genetic alterations in signaling pathways associated with drug resistance and sensitivity, likely contributed to the variable responses to TKIs. Notably, TP53 was the most frequently mutated gene, with alterations detected in 65% of patients. In addition, the results from the KEGG pathways analysis showed that the variants were highly clustered in several signaling pathways such as PI3K-Akt pathway, EGFR tyrosine kinase inhibitor resistance and ErbB pathway.
Our results suggest that in addition to mutational status, performing functional sensitivity screening is critical for identifying appropriate treatment strategies with TKIs. These results underscore the importance of considering drug sensitivity, off-target effects, and patient-specific genetic profiles to optimize treatment strategies for NSCLC.",
publisher = "EMBO press",
journal = "Abstract book: Molecular mechanisms in signal transduction and cancer: EMBO-FEBS Lecture course; 2024 Aug 18-26; Spetses, Greece",
title = "Functional diagnostics in non-small cell lung carcinoma - the potential for personalized approach",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7032"
}

Lupšić, E., Dinić, J., Dragoj, M., Jovanović Stojanov, S., Stepanović, A., Pajović, M., Mohr, T., Glumac, S., Marić, D., Ercegovac, M., Podolski-Renić, A.,& Pešić, M.. (2024). Functional diagnostics in non-small cell lung carcinoma - the potential for personalized approach. in Abstract book: Molecular mechanisms in signal transduction and cancer: EMBO-FEBS Lecture course; 2024 Aug 18-26; Spetses, Greece
EMBO press..
https://hdl.handle.net/21.15107/rcub_ibiss_7032

Lupšić E, Dinić J, Dragoj M, Jovanović Stojanov S, Stepanović A, Pajović M, Mohr T, Glumac S, Marić D, Ercegovac M, Podolski-Renić A, Pešić M. Functional diagnostics in non-small cell lung carcinoma - the potential for personalized approach. in Abstract book: Molecular mechanisms in signal transduction and cancer: EMBO-FEBS Lecture course; 2024 Aug 18-26; Spetses, Greece. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_7032 .

Lupšić, Ema, Dinić, Jelena, Dragoj, Miodrag, Jovanović Stojanov, Sofija, Stepanović, Ana, Pajović, Milica, Mohr, Thomas, Glumac, Sofija, Marić, Dragana, Ercegovac, Maja, Podolski-Renić, Ana, Pešić, Milica, "Functional diagnostics in non-small cell lung carcinoma - the potential for personalized approach" in Abstract book: Molecular mechanisms in signal transduction and cancer: EMBO-FEBS Lecture course; 2024 Aug 18-26; Spetses, Greece (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_7032 .

TY  - CONF
AU  - Marković, Vanja
AU  - Đuretanović, Simona
AU  - Roljić, Rajko
AU  - Nikolić, Vera
AU  - Zorić, Katarina
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7028
AB  - The Balkans is Austropotamobius torrentium (Schrank, 1803) hotspot. In its central parts (Serbia) three main stone crayfish phylogroups are present: CSE (Central and South-East European), SB (Southern Balkans) and LD (Lika and Dalmatia). In order to assess the morphological variability of the stone crayfish we analyzed ten populations from the central Balkans. A total of 145 adult crayfish (total length over 60 mm) were measured during our 2017 field study and 21 linear parameters and individual weight were taken. All measurements were standardized by postorbital length. Of analyzed crayfish 80 were males and 65 females. Performed non-parametric tests (Kruskal-Wallis) have shown that males and females differ in majority of parameters, including those related to weight. Discriminant analysis showed that abdominal and claw widths were the most important for sex separation, with the first one having larger values in females, while the second one being larger in males. Regarding populations, non-parametric tests showed that only stable parameters among male populations were a few characteristics of abdomen, claws and weight, while in female populations those were a bit more numerous. Canonical discriminate analysis was used to assess spatial morphological variability among studied populations (10 populations for males, and 8 for females). The results have shown that separation among male samples is more pronounced than in females. Alongside the first root a clear distinction of Uvac (CSE phylogroup) and Rasina (SB phylogroup) populations from the rest can be observed, while along the second root these two populations differ. Considering that analyzed populations belong to all three main phylogroups our results suggest that morphological variability of the stone crayfish is related more to specific environmental conditions (adaptations) than to separate phylogenetic lineages.
PB  - Zagreb: Croatian Ecological Society
C3  - Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia
T1  - Morphological variability among populations of Austropotamobius torrentium (Schrank, 1803) from central Balkan
SP  - 114
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_7028
ER  - 

@conference{
author = "Marković, Vanja and Đuretanović, Simona and Roljić, Rajko and Nikolić, Vera and Zorić, Katarina",
year = "2024",
abstract = "The Balkans is Austropotamobius torrentium (Schrank, 1803) hotspot. In its central parts (Serbia) three main stone crayfish phylogroups are present: CSE (Central and South-East European), SB (Southern Balkans) and LD (Lika and Dalmatia). In order to assess the morphological variability of the stone crayfish we analyzed ten populations from the central Balkans. A total of 145 adult crayfish (total length over 60 mm) were measured during our 2017 field study and 21 linear parameters and individual weight were taken. All measurements were standardized by postorbital length. Of analyzed crayfish 80 were males and 65 females. Performed non-parametric tests (Kruskal-Wallis) have shown that males and females differ in majority of parameters, including those related to weight. Discriminant analysis showed that abdominal and claw widths were the most important for sex separation, with the first one having larger values in females, while the second one being larger in males. Regarding populations, non-parametric tests showed that only stable parameters among male populations were a few characteristics of abdomen, claws and weight, while in female populations those were a bit more numerous. Canonical discriminate analysis was used to assess spatial morphological variability among studied populations (10 populations for males, and 8 for females). The results have shown that separation among male samples is more pronounced than in females. Alongside the first root a clear distinction of Uvac (CSE phylogroup) and Rasina (SB phylogroup) populations from the rest can be observed, while along the second root these two populations differ. Considering that analyzed populations belong to all three main phylogroups our results suggest that morphological variability of the stone crayfish is related more to specific environmental conditions (adaptations) than to separate phylogenetic lineages.",
publisher = "Zagreb: Croatian Ecological Society",
journal = "Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia",
title = "Morphological variability among populations of Austropotamobius torrentium (Schrank, 1803) from central Balkan",
pages = "114-115",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7028"
}

Marković, V., Đuretanović, S., Roljić, R., Nikolić, V.,& Zorić, K.. (2024). Morphological variability among populations of Austropotamobius torrentium (Schrank, 1803) from central Balkan. in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia
Zagreb: Croatian Ecological Society., 114-115.
https://hdl.handle.net/21.15107/rcub_ibiss_7028

Marković V, Đuretanović S, Roljić R, Nikolić V, Zorić K. Morphological variability among populations of Austropotamobius torrentium (Schrank, 1803) from central Balkan. in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia. 2024;:114-115.
https://hdl.handle.net/21.15107/rcub_ibiss_7028 .

Marković, Vanja, Đuretanović, Simona, Roljić, Rajko, Nikolić, Vera, Zorić, Katarina, "Morphological variability among populations of Austropotamobius torrentium (Schrank, 1803) from central Balkan" in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia (2024):114-115,
https://hdl.handle.net/21.15107/rcub_ibiss_7028 .

TY  - JOUR
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Lazarević, Jelica
AU  - Perić Mataruga, Vesna
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5361
AB  - Cadmium pollution is becoming an increasing problem, especially in parts of the world that have developed industries. To consider the potentially harmful effects of cadmium, we need to examine changes at all different levels of biological organization. The main goal of this study was to detect a possible change in the percentage of midgut mass relative to larval mass (PMM) and determine the plasticity of this trait and the correlations between midgut enzymes and PMM under stress conditions. Fourth-instar larvae were exposed to acute and chronic effects of two cadmium concentrations, 10 and 30 μg Cd/g dry food, as well as a three-day recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute effects and chronic treatment at 30 μg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium action, while in chronic treatment, the genetic factor (egg mass) plays a crucial role in the change of PMM. According to the index of plasticity, distinct phenotypes were not produced. Significant correlations were shown between PMM and trypsin (Tryp) and leucine aminopeptidases (LAP) at acute and chronic treatment with higher cadmium concentrations, while significant correlations between proteases and PMM were detected at lower metal concentrations (Acute10 and Chronic10 and 30 μg Cd/g dry food). In contrast to chronic treatment, egg masses respond more uniformly by reducing PMM during the short-term effect of cadmium. Finally, we can conclude that, as an addition to biochemical and molecular research, PMM can be used for studying the cadmium effects to gain a better insight into the state of the organism under stress conditions.
PB  - Lahore: Zoological Society of Pakistan
T2  - Pakistan Journal of Zoology
T1  - The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae
IS  - 1
VL  - 56
DO  - 10.17582/journal.pjz/20211019131007
SP  - 339
EP  - 346
ER  - 

@article{
author = "Vlahović, Milena and Matić, Dragana and Mrdaković, Marija and Ilijin, Larisa and Grčić, Anja and Filipović, Aleksandra and Lazarević, Jelica and Perić Mataruga, Vesna",
year = "2024",
abstract = "Cadmium pollution is becoming an increasing problem, especially in parts of the world that have developed industries. To consider the potentially harmful effects of cadmium, we need to examine changes at all different levels of biological organization. The main goal of this study was to detect a possible change in the percentage of midgut mass relative to larval mass (PMM) and determine the plasticity of this trait and the correlations between midgut enzymes and PMM under stress conditions. Fourth-instar larvae were exposed to acute and chronic effects of two cadmium concentrations, 10 and 30 μg Cd/g dry food, as well as a three-day recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute effects and chronic treatment at 30 μg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium action, while in chronic treatment, the genetic factor (egg mass) plays a crucial role in the change of PMM. According to the index of plasticity, distinct phenotypes were not produced. Significant correlations were shown between PMM and trypsin (Tryp) and leucine aminopeptidases (LAP) at acute and chronic treatment with higher cadmium concentrations, while significant correlations between proteases and PMM were detected at lower metal concentrations (Acute10 and Chronic10 and 30 μg Cd/g dry food). In contrast to chronic treatment, egg masses respond more uniformly by reducing PMM during the short-term effect of cadmium. Finally, we can conclude that, as an addition to biochemical and molecular research, PMM can be used for studying the cadmium effects to gain a better insight into the state of the organism under stress conditions.",
publisher = "Lahore: Zoological Society of Pakistan",
journal = "Pakistan Journal of Zoology",
title = "The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae",
number = "1",
volume = "56",
doi = "10.17582/journal.pjz/20211019131007",
pages = "339-346"
}

Vlahović, M., Matić, D., Mrdaković, M., Ilijin, L., Grčić, A., Filipović, A., Lazarević, J.,& Perić Mataruga, V.. (2024). The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae. in Pakistan Journal of Zoology
Lahore: Zoological Society of Pakistan., 56(1), 339-346.
https://doi.org/10.17582/journal.pjz/20211019131007

Vlahović M, Matić D, Mrdaković M, Ilijin L, Grčić A, Filipović A, Lazarević J, Perić Mataruga V. The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae. in Pakistan Journal of Zoology. 2024;56(1):339-346.
doi:10.17582/journal.pjz/20211019131007 .

Vlahović, Milena, Matić, Dragana, Mrdaković, Marija, Ilijin, Larisa, Grčić, Anja, Filipović, Aleksandra, Lazarević, Jelica, Perić Mataruga, Vesna, "The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae" in Pakistan Journal of Zoology, 56, no. 1 (2024):339-346,
https://doi.org/10.17582/journal.pjz/20211019131007 . .

TY  - CONF
AU  - Đuretanović, Simona
AU  - Stojanović, Katarina
AU  - Marković, Vanja
AU  - Zorić, Katarina
AU  - Simović, Predrag
AU  - Živić, Ivana
AU  - Simić, Vladica
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7030
AB  - Our study aims to build upon a prior studies of crayfish distribution by consolidating findings from our fifteen years field research and summarizing existing published data. In Serbian freshwater ecosystems, we have identified the stone crayfish Austropotamobius torrentium, the noble crayfish Astacus astacus, and the narrow-clawed crayfish Pontastacus leptodactylus as native species. Unfortunately, our ecosystems have been penetrated by two invasive species: the spiny-cheek crayfish Faxonius limosus, first discovered in 2004, and the signal crayfish Pacifastacus leniusculus, more recently found in 2020. Fifteen years since the last research, we have observed that the most commonly found native crayfish is the stone crayfish, documented at 206 sites in 131 freshwater ecosystems, followed by the noble crayfish (46 sites in 31 freshwater ecosystems) and the narrow-clawed crayfish (22 sites in 11 freshwater ecosystems). Field observations have shown that changes in habitat and the increasing impact of climate change (significant droughts and floods over the past decade), primarily affect native crayfish populations. Understanding species distribution is fundamental to a wide range of biological research. Gathering additional data on species distribution is essential for enhancing our understanding of biodiversity, the functioning of aquatic ecosystems, conservation planning, climate change adaptation, and the management of invasive species. Moreover, distribution data is crucial for monitoring endangered species, such as the stone and noble crayfish, which are strictly protected under the Rulebook on the Proclamation and Protection of Strictly Protected and Protected Wild Species of Plants, Animals, and Mushrooms ("Official Gazette of RS" no. 5/2010, 47/2011, 32/2016 and 98/2016).
PB  - Zagreb: Croatian Ecological Society
C3  - Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia
T1  - Freshwater crayfish in Serbia: Update on the distribution
SP  - 99
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_7030
ER  - 

@conference{
author = "Đuretanović, Simona and Stojanović, Katarina and Marković, Vanja and Zorić, Katarina and Simović, Predrag and Živić, Ivana and Simić, Vladica",
year = "2024",
abstract = "Our study aims to build upon a prior studies of crayfish distribution by consolidating findings from our fifteen years field research and summarizing existing published data. In Serbian freshwater ecosystems, we have identified the stone crayfish Austropotamobius torrentium, the noble crayfish Astacus astacus, and the narrow-clawed crayfish Pontastacus leptodactylus as native species. Unfortunately, our ecosystems have been penetrated by two invasive species: the spiny-cheek crayfish Faxonius limosus, first discovered in 2004, and the signal crayfish Pacifastacus leniusculus, more recently found in 2020. Fifteen years since the last research, we have observed that the most commonly found native crayfish is the stone crayfish, documented at 206 sites in 131 freshwater ecosystems, followed by the noble crayfish (46 sites in 31 freshwater ecosystems) and the narrow-clawed crayfish (22 sites in 11 freshwater ecosystems). Field observations have shown that changes in habitat and the increasing impact of climate change (significant droughts and floods over the past decade), primarily affect native crayfish populations. Understanding species distribution is fundamental to a wide range of biological research. Gathering additional data on species distribution is essential for enhancing our understanding of biodiversity, the functioning of aquatic ecosystems, conservation planning, climate change adaptation, and the management of invasive species. Moreover, distribution data is crucial for monitoring endangered species, such as the stone and noble crayfish, which are strictly protected under the Rulebook on the Proclamation and Protection of Strictly Protected and Protected Wild Species of Plants, Animals, and Mushrooms ("Official Gazette of RS" no. 5/2010, 47/2011, 32/2016 and 98/2016).",
publisher = "Zagreb: Croatian Ecological Society",
journal = "Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia",
title = "Freshwater crayfish in Serbia: Update on the distribution",
pages = "99",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7030"
}

Đuretanović, S., Stojanović, K., Marković, V., Zorić, K., Simović, P., Živić, I.,& Simić, V.. (2024). Freshwater crayfish in Serbia: Update on the distribution. in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia
Zagreb: Croatian Ecological Society., 99.
https://hdl.handle.net/21.15107/rcub_ibiss_7030

Đuretanović S, Stojanović K, Marković V, Zorić K, Simović P, Živić I, Simić V. Freshwater crayfish in Serbia: Update on the distribution. in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia. 2024;:99.
https://hdl.handle.net/21.15107/rcub_ibiss_7030 .

Đuretanović, Simona, Stojanović, Katarina, Marković, Vanja, Zorić, Katarina, Simović, Predrag, Živić, Ivana, Simić, Vladica, "Freshwater crayfish in Serbia: Update on the distribution" in Book of abstracts: International Association of Astacology Symposium 24: IAA24; 2024 Sep 16-20; Zagreb, Croatia (2024):99,
https://hdl.handle.net/21.15107/rcub_ibiss_7030 .

TY  - CONF
AU  - Koračak, Ljiljana
AU  - Selaković, Milica
AU  - Lupšić, Ema
AU  - Podolski-Renić, Ana
AU  - Selaković, Života
AU  - Novaković, Miroslav
AU  - Pešić, Milica
AU  - Opsenica, Igor M.
PY  - 2024
UR  - https://www.efmc-ismc.org/v2/data/1725266112EFMC-ISMC-2024-BoA-web-FINAL.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7024
AB  - Artesunate, a derivative of the natural product artemisinin, displays anticancer activity against a wide variety of cancer cell lines. While natural products serve as excellent lead compounds, additional synthetic transformations are often necessary to achieve ‘‘tailor-made’’ final structure.1 The concept of hybrid molecules with fusion of two different pharmacophores where one or both may be inspired by naturally occurring molecules allowing significant enhancements in physicochemical and pharmacokinetic roperties.2 
This study focused on synthesizing novel hybrid molecules combining artesunate and a pyrimidine scaffold to assess their efficacy against both sensitive and multidrug-resistant (MDR) human non-small cell lung carcinoma (NSCLC) cells. Several synthesized hybrids exhibited higher activity against both examined cell lines than the parent compounds. In addition, increased sensitivity of MDR NSCLC cells to doxorubicin was observed when it was applied in combination with novel hybrid molecules as well as the inhibition of P-glycoprotein activity.

References:
1) Z. Guo, Acta Pharm. Sin. B, 2017, 7, 119–136
2) C. Borsari, D. J. Trader, A. Tait and M. P. Costi, J. Med. Chem., 2020, 63, 1908–1928
PB  - Belgium: Scientific Conference Producers - LD Organisation srl
C3  - Book of Abstracts: EFSM-ISMC: International Symposium on Medicinal Chemistry; 2024 Sep 1-5; Rome, Italy
T1  - Synthesis of Artesunate-Pyrimidine Hybrids and Evaluation of Their Activity Against Multidrug-Resistant Cancer Cells
SP  - 345
ER  - 

@conference{
author = "Koračak, Ljiljana and Selaković, Milica and Lupšić, Ema and Podolski-Renić, Ana and Selaković, Života and Novaković, Miroslav and Pešić, Milica and Opsenica, Igor M.",
year = "2024",
abstract = "Artesunate, a derivative of the natural product artemisinin, displays anticancer activity against a wide variety of cancer cell lines. While natural products serve as excellent lead compounds, additional synthetic transformations are often necessary to achieve ‘‘tailor-made’’ final structure.1 The concept of hybrid molecules with fusion of two different pharmacophores where one or both may be inspired by naturally occurring molecules allowing significant enhancements in physicochemical and pharmacokinetic roperties.2 
This study focused on synthesizing novel hybrid molecules combining artesunate and a pyrimidine scaffold to assess their efficacy against both sensitive and multidrug-resistant (MDR) human non-small cell lung carcinoma (NSCLC) cells. Several synthesized hybrids exhibited higher activity against both examined cell lines than the parent compounds. In addition, increased sensitivity of MDR NSCLC cells to doxorubicin was observed when it was applied in combination with novel hybrid molecules as well as the inhibition of P-glycoprotein activity.

References:
1) Z. Guo, Acta Pharm. Sin. B, 2017, 7, 119–136
2) C. Borsari, D. J. Trader, A. Tait and M. P. Costi, J. Med. Chem., 2020, 63, 1908–1928",
publisher = "Belgium: Scientific Conference Producers - LD Organisation srl",
journal = "Book of Abstracts: EFSM-ISMC: International Symposium on Medicinal Chemistry; 2024 Sep 1-5; Rome, Italy",
title = "Synthesis of Artesunate-Pyrimidine Hybrids and Evaluation of Their Activity Against Multidrug-Resistant Cancer Cells",
pages = "345"
}

Koračak, L., Selaković, M., Lupšić, E., Podolski-Renić, A., Selaković, Ž., Novaković, M., Pešić, M.,& Opsenica, I. M.. (2024). Synthesis of Artesunate-Pyrimidine Hybrids and Evaluation of Their Activity Against Multidrug-Resistant Cancer Cells. in Book of Abstracts: EFSM-ISMC: International Symposium on Medicinal Chemistry; 2024 Sep 1-5; Rome, Italy
Belgium: Scientific Conference Producers - LD Organisation srl., 345.

Koračak L, Selaković M, Lupšić E, Podolski-Renić A, Selaković Ž, Novaković M, Pešić M, Opsenica IM. Synthesis of Artesunate-Pyrimidine Hybrids and Evaluation of Their Activity Against Multidrug-Resistant Cancer Cells. in Book of Abstracts: EFSM-ISMC: International Symposium on Medicinal Chemistry; 2024 Sep 1-5; Rome, Italy. 2024;:345..

Koračak, Ljiljana, Selaković, Milica, Lupšić, Ema, Podolski-Renić, Ana, Selaković, Života, Novaković, Miroslav, Pešić, Milica, Opsenica, Igor M., "Synthesis of Artesunate-Pyrimidine Hybrids and Evaluation of Their Activity Against Multidrug-Resistant Cancer Cells" in Book of Abstracts: EFSM-ISMC: International Symposium on Medicinal Chemistry; 2024 Sep 1-5; Rome, Italy (2024):345.

TY  - CONF
AU  - Lazarević, Milica
AU  - Stegnjaić, Goran
AU  - Stanisavljević, Suzana
AU  - Nikolovski, Neda
AU  - Momčilović, Miljana
AU  - Dimitrijević, Mirjana
AU  - Fraser, Graeme L
AU  - Miljković, Đorđe
AU  - Jevtić, Bojan
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7021
AB  - Innate lymphoid cells type 3 (ILC3) play a central role in maintaining intestinal homeostasis and regulating the balance between effector T cell populations and regulatory T cells (Treg) in the gut milieu. Dysregulation of this balance, which is strongly influenced by the gut microbiota and dietary components, is associated with autoimmune diseases such as multiple sclerosis (MS). Intestinal ILC3 are activated by dietary compounds like short-chain fatty acids (SCFA), which are produced by gut bacteria. SCFA, acting via the free fatty acid receptor 2 (FFAR2), stimulate ILC3 proliferation, IL-22 and IL-2 production in the small intestine. The secretion of IL-22 by ILC3 supports the integrity of the intestinal barrier and the balance of the immune system, while IL-2 enhances the activity of Treg and thus improves immune regulation in the intestine.
In this study, the efficacy of a FFAR2 agonist - Cpd1 as evaluated in a mouse model of chronic experimental autoimmune encephalomyelitis (EAE). Treatment with Cpd1 efficiently ameliorated EAE and showed significant attenuation of CNS inflammation and lessened infiltration of immune cells into the spinal cord. Flow cytometric analysis of immune cells in the spinal cord showed that the number of CD4+ T cells, Th1, and Th17 cells decreased with Cpd1 treatment. In addition, Cpd1 treatment in EAE mice led to changes in the composition of immune cells in the lamina propria of the small intestine, which was characterized by an increased proportion of Treg and IL-22-producing ILC3 and a decrease in IL-17-
expressing ILC3, while there was no difference in the proportion of IL-2-producing ILC3 cells between Cpd1-treated and control mice. Consistent with this, Cpd1 treatment also altered the composition of the microbiota in the EAE model. In summary, these data indicate that activation of immune-regulatory, gut-resident ILC3 cells by FFAR2 agonists modulates the autoimmune response local to the small intestine as well as in distal tissues such as the spinal cord. These findings highlight the potential therapeutic use of FFAR2 agonists in the treatment of autoimmune diseases.
PB  - John Wiley and Sons
C3  - 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland
T1  - Pharmacological activation of free fatty acid receptor 2 in intestinal type 3 innate lymphoid cells ameliorates experimental autoimmune encephalomyelitis
DO  - 10.1002/eji.202470200
SP  - 23
ER  - 

@conference{
author = "Lazarević, Milica and Stegnjaić, Goran and Stanisavljević, Suzana and Nikolovski, Neda and Momčilović, Miljana and Dimitrijević, Mirjana and Fraser, Graeme L and Miljković, Đorđe and Jevtić, Bojan",
year = "2024",
abstract = "Innate lymphoid cells type 3 (ILC3) play a central role in maintaining intestinal homeostasis and regulating the balance between effector T cell populations and regulatory T cells (Treg) in the gut milieu. Dysregulation of this balance, which is strongly influenced by the gut microbiota and dietary components, is associated with autoimmune diseases such as multiple sclerosis (MS). Intestinal ILC3 are activated by dietary compounds like short-chain fatty acids (SCFA), which are produced by gut bacteria. SCFA, acting via the free fatty acid receptor 2 (FFAR2), stimulate ILC3 proliferation, IL-22 and IL-2 production in the small intestine. The secretion of IL-22 by ILC3 supports the integrity of the intestinal barrier and the balance of the immune system, while IL-2 enhances the activity of Treg and thus improves immune regulation in the intestine.
In this study, the efficacy of a FFAR2 agonist - Cpd1 as evaluated in a mouse model of chronic experimental autoimmune encephalomyelitis (EAE). Treatment with Cpd1 efficiently ameliorated EAE and showed significant attenuation of CNS inflammation and lessened infiltration of immune cells into the spinal cord. Flow cytometric analysis of immune cells in the spinal cord showed that the number of CD4+ T cells, Th1, and Th17 cells decreased with Cpd1 treatment. In addition, Cpd1 treatment in EAE mice led to changes in the composition of immune cells in the lamina propria of the small intestine, which was characterized by an increased proportion of Treg and IL-22-producing ILC3 and a decrease in IL-17-
expressing ILC3, while there was no difference in the proportion of IL-2-producing ILC3 cells between Cpd1-treated and control mice. Consistent with this, Cpd1 treatment also altered the composition of the microbiota in the EAE model. In summary, these data indicate that activation of immune-regulatory, gut-resident ILC3 cells by FFAR2 agonists modulates the autoimmune response local to the small intestine as well as in distal tissues such as the spinal cord. These findings highlight the potential therapeutic use of FFAR2 agonists in the treatment of autoimmune diseases.",
publisher = "John Wiley and Sons",
journal = "7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland",
title = "Pharmacological activation of free fatty acid receptor 2 in intestinal type 3 innate lymphoid cells ameliorates experimental autoimmune encephalomyelitis",
doi = "10.1002/eji.202470200",
pages = "23"
}

Lazarević, M., Stegnjaić, G., Stanisavljević, S., Nikolovski, N., Momčilović, M., Dimitrijević, M., Fraser, G. L., Miljković, Đ.,& Jevtić, B.. (2024). Pharmacological activation of free fatty acid receptor 2 in intestinal type 3 innate lymphoid cells ameliorates experimental autoimmune encephalomyelitis. in 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland
John Wiley and Sons., 23.
https://doi.org/10.1002/eji.202470200

Lazarević M, Stegnjaić G, Stanisavljević S, Nikolovski N, Momčilović M, Dimitrijević M, Fraser GL, Miljković Đ, Jevtić B. Pharmacological activation of free fatty acid receptor 2 in intestinal type 3 innate lymphoid cells ameliorates experimental autoimmune encephalomyelitis. in 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland. 2024;:23.
doi:10.1002/eji.202470200 .

Lazarević, Milica, Stegnjaić, Goran, Stanisavljević, Suzana, Nikolovski, Neda, Momčilović, Miljana, Dimitrijević, Mirjana, Fraser, Graeme L, Miljković, Đorđe, Jevtić, Bojan, "Pharmacological activation of free fatty acid receptor 2 in intestinal type 3 innate lymphoid cells ameliorates experimental autoimmune encephalomyelitis" in 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland (2024):23,
https://doi.org/10.1002/eji.202470200 . .

TY  - CONF
AU  - Potrebić, Milica
AU  - Vidonja Uzelac, Teodora
AU  - Pavković, Željko
AU  - Nikolić Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Dubljević, Olga
AU  - Srbovan, Maja
AU  - Blagojević, Duško
AU  - Pešić, Vesna
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7019
AB  - The influence of psychosocial stress on antioxidant enzymes (AOE) in the central nervous system is associated with mood disorders. To
unravel systemic effects of psychosocial stress we aimed to investigate AOE activity in peripheral organs and erythrocytes of peripubertal rats due to social
isolation during peripuberty. Social play with peers during this period is highly rewarding activity abundant in the young of most mammalian species.
Absence of peer social support is associated with depression. Male peripubertal Wistar Han rats were either group-housed (3/cage) or single-housed (1/cage)
during early postnatal (P) days (P29-P36) and early-to-late (P29-P43) adolescence. To assess whether social environment influences AOE activity to additional
stress, half of the animals from both age groups underwent overnight fasting. Tissue samples (liver, kidney, heart) and erythrocytes were collected at P36
and P43. AOE activity (glutathione peroxidase, superoxide dismutases, catalase, and glutathione reductase) was measured spectrophotometrically.
Glutathione reductase and catalase were unaffected by social isolation in both age groups in all examined organs. The influence of social isolation on the
AOE activity after the overnight fasting was tissue- and enzyme-specific and was dependent on the maturational phase. Erythrocyte superoxide dismutase
was the only enzyme that showed increased activity due to the social isolation, regardless of the presence of an additional stress. Social isolation in
adolescence modifies AOE activity in peripheral organs and erythrocytes, shaping the response of the enzymes to additional stress. Erythrocyte superoxide
dismutase could be a biomarker of social stress-related mood disorders in adolescence.
C3  - FENS Forum 2024; 2024 Jun 25-29; Vienna, Austria
T1  - Altered activities of antioxidant enzymes in peripheral organs and erythrocytes due to social isolation in peripuberty: findings from a rodent study
ER  - 

@conference{
author = "Potrebić, Milica and Vidonja Uzelac, Teodora and Pavković, Željko and Nikolić Kokić, Aleksandra and Oreščanin Dušić, Zorana and Dubljević, Olga and Srbovan, Maja and Blagojević, Duško and Pešić, Vesna",
year = "2024",
abstract = "The influence of psychosocial stress on antioxidant enzymes (AOE) in the central nervous system is associated with mood disorders. To
unravel systemic effects of psychosocial stress we aimed to investigate AOE activity in peripheral organs and erythrocytes of peripubertal rats due to social
isolation during peripuberty. Social play with peers during this period is highly rewarding activity abundant in the young of most mammalian species.
Absence of peer social support is associated with depression. Male peripubertal Wistar Han rats were either group-housed (3/cage) or single-housed (1/cage)
during early postnatal (P) days (P29-P36) and early-to-late (P29-P43) adolescence. To assess whether social environment influences AOE activity to additional
stress, half of the animals from both age groups underwent overnight fasting. Tissue samples (liver, kidney, heart) and erythrocytes were collected at P36
and P43. AOE activity (glutathione peroxidase, superoxide dismutases, catalase, and glutathione reductase) was measured spectrophotometrically.
Glutathione reductase and catalase were unaffected by social isolation in both age groups in all examined organs. The influence of social isolation on the
AOE activity after the overnight fasting was tissue- and enzyme-specific and was dependent on the maturational phase. Erythrocyte superoxide dismutase
was the only enzyme that showed increased activity due to the social isolation, regardless of the presence of an additional stress. Social isolation in
adolescence modifies AOE activity in peripheral organs and erythrocytes, shaping the response of the enzymes to additional stress. Erythrocyte superoxide
dismutase could be a biomarker of social stress-related mood disorders in adolescence.",
journal = "FENS Forum 2024; 2024 Jun 25-29; Vienna, Austria",
title = "Altered activities of antioxidant enzymes in peripheral organs and erythrocytes due to social isolation in peripuberty: findings from a rodent study"
}

Potrebić, M., Vidonja Uzelac, T., Pavković, Ž., Nikolić Kokić, A., Oreščanin Dušić, Z., Dubljević, O., Srbovan, M., Blagojević, D.,& Pešić, V.. (2024). Altered activities of antioxidant enzymes in peripheral organs and erythrocytes due to social isolation in peripuberty: findings from a rodent study. in FENS Forum 2024; 2024 Jun 25-29; Vienna, Austria.

Potrebić M, Vidonja Uzelac T, Pavković Ž, Nikolić Kokić A, Oreščanin Dušić Z, Dubljević O, Srbovan M, Blagojević D, Pešić V. Altered activities of antioxidant enzymes in peripheral organs and erythrocytes due to social isolation in peripuberty: findings from a rodent study. in FENS Forum 2024; 2024 Jun 25-29; Vienna, Austria. 2024;..

Potrebić, Milica, Vidonja Uzelac, Teodora, Pavković, Željko, Nikolić Kokić, Aleksandra, Oreščanin Dušić, Zorana, Dubljević, Olga, Srbovan, Maja, Blagojević, Duško, Pešić, Vesna, "Altered activities of antioxidant enzymes in peripheral organs and erythrocytes due to social isolation in peripuberty: findings from a rodent study" in FENS Forum 2024; 2024 Jun 25-29; Vienna, Austria (2024).

TY  - JOUR
AU  - Nikolić, Andrea M.
AU  - Koračak, Ljiljana
AU  - Lupšić, Ema
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Opsenica, Igor M.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7022
AB  - From nucleobases to FDA approved drugs, pyrimidine is present in numerous molecules important for our lives. In terms of medicinal chemistry, pyrimidine moiety was shown to contribute to different bioactivity modes (Curr. Med. Chem. 26 (2019) 7166). Derivatization of natural products with structural scaffolds that also exhibit bioactivity is a prominent way to improve the pharmacological properties of compounds. With this in mind, we designed hybrid structures of artesunic acid and pyrimidine in order to obtain potent molecules that have desirable properties of both pharmacophores. A library of compounds was obtained in a facile way which was then screened for anticancer activity. Based on the biological data, it was shown that utilizing pyrimidine for derivatization of artesunic acid contributed to the improved activity and selectivity against human non-small cell lung carcinoma cells.
PB  - Prague: Venice Praha, s.r.o.
T2  - Programme and Book of abstracts: 20th Blue Danube Symposium on Heterocycles in Chemistry; 2024 Aug 25-28; Prague, Czech Republic
T1  - The Use of Pyrimidines in Medicinal Chemistry: Derivatives with Artesunic Acid
SP  - 101
ER  - 

@article{
author = "Nikolić, Andrea M. and Koračak, Ljiljana and Lupšić, Ema and Podolski-Renić, Ana and Pešić, Milica and Opsenica, Igor M.",
year = "2024",
abstract = "From nucleobases to FDA approved drugs, pyrimidine is present in numerous molecules important for our lives. In terms of medicinal chemistry, pyrimidine moiety was shown to contribute to different bioactivity modes (Curr. Med. Chem. 26 (2019) 7166). Derivatization of natural products with structural scaffolds that also exhibit bioactivity is a prominent way to improve the pharmacological properties of compounds. With this in mind, we designed hybrid structures of artesunic acid and pyrimidine in order to obtain potent molecules that have desirable properties of both pharmacophores. A library of compounds was obtained in a facile way which was then screened for anticancer activity. Based on the biological data, it was shown that utilizing pyrimidine for derivatization of artesunic acid contributed to the improved activity and selectivity against human non-small cell lung carcinoma cells.",
publisher = "Prague: Venice Praha, s.r.o.",
journal = "Programme and Book of abstracts: 20th Blue Danube Symposium on Heterocycles in Chemistry; 2024 Aug 25-28; Prague, Czech Republic",
title = "The Use of Pyrimidines in Medicinal Chemistry: Derivatives with Artesunic Acid",
pages = "101"
}

Nikolić, A. M., Koračak, L., Lupšić, E., Podolski-Renić, A., Pešić, M.,& Opsenica, I. M.. (2024). The Use of Pyrimidines in Medicinal Chemistry: Derivatives with Artesunic Acid. in Programme and Book of abstracts: 20th Blue Danube Symposium on Heterocycles in Chemistry; 2024 Aug 25-28; Prague, Czech Republic
Prague: Venice Praha, s.r.o.., 101.

Nikolić AM, Koračak L, Lupšić E, Podolski-Renić A, Pešić M, Opsenica IM. The Use of Pyrimidines in Medicinal Chemistry: Derivatives with Artesunic Acid. in Programme and Book of abstracts: 20th Blue Danube Symposium on Heterocycles in Chemistry; 2024 Aug 25-28; Prague, Czech Republic. 2024;:101..

Nikolić, Andrea M., Koračak, Ljiljana, Lupšić, Ema, Podolski-Renić, Ana, Pešić, Milica, Opsenica, Igor M., "The Use of Pyrimidines in Medicinal Chemistry: Derivatives with Artesunic Acid" in Programme and Book of abstracts: 20th Blue Danube Symposium on Heterocycles in Chemistry; 2024 Aug 25-28; Prague, Czech Republic (2024):101.

TY  - CONF
AU  - Jonić, Natalija
AU  - Koprivica, Ivan
AU  - Mićanović, Dragica
AU  - Saksida, Tamara
AU  - Jevtić, Bojan
AU  - Fraser, Graeme L
AU  - Miljković, Đorđe
AU  - Stojanović, Ivana D.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7023
AB  - Type 1 diabetes (T1D) is a chronic autoimmune disease that usually develops in early childhood. The recent market approval of the anti-CD3 monoclonal antibody (teplizumab) is a major advance in protecting at-risk patients from the onset  of T1D . In addition to the presence of autoantibodies, T1D-prone individuals exhibit alteration in the gut microbiota and the intestinal immune response. As intestinal epithelial and immune cells express receptors for free fatty acids (FFAR), and it was shown that stimulation of FFAR2 can down-regulate inflammation during Crohn’s disease, we have tested the efficacy of the novel agonist for FFAR2 (Cpd1) in the prevention of T1D induced by five injections of low-dose of streptozotocin in C57BL/6 mice. Oral application of Cpd1 (applied from the 1st day of T1D induction for 20 days in total) resulted in a significant reduction of T1D incidence and lowered mean glycemia levels in the treated mice. Accordingly, these mice had lower insulitis in the pancreas and higher insulin production. Ex vivo analysis on the 8th day post T1D induction showed higher proportions of innate lymphoid cell type 3 (ILC3) that produced IL-2 within the small intestine (SI) lamina propria of Cpd1-treated mice. The increase in IL-2 correlated with  higher Treg proportions detected on day 12 in the SI lamina propria. Similar findings were observed in NOD mice, a spontaneous T1D model, treated with Cpd1 on a daily basis (from 8 weeks until 12 weeks of age). Cpd1 increased the presence of ILC3 in the SI lamina propria of NOD mice, and more specifically, the proportion of Nkp46− ILC3. In total, these results demonstrate that Cpd1 increased the population of ILC3 and Treg by activating FFAR2, thereby modulating the anti-inflammatory immune response and protecting the pancreas from the imposed damage.
PB  - John Wiley and Sons
C3  - 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland
T1  - Novel FFAR2 agonist prevents development of type 1 diabetes in C57BL/6 mice
DO  - 10.1002/eji.202470200
SP  - 1210
ER  - 

@conference{
author = "Jonić, Natalija and Koprivica, Ivan and Mićanović, Dragica and Saksida, Tamara and Jevtić, Bojan and Fraser, Graeme L and Miljković, Đorđe and Stojanović, Ivana D.",
year = "2024",
abstract = "Type 1 diabetes (T1D) is a chronic autoimmune disease that usually develops in early childhood. The recent market approval of the anti-CD3 monoclonal antibody (teplizumab) is a major advance in protecting at-risk patients from the onset  of T1D . In addition to the presence of autoantibodies, T1D-prone individuals exhibit alteration in the gut microbiota and the intestinal immune response. As intestinal epithelial and immune cells express receptors for free fatty acids (FFAR), and it was shown that stimulation of FFAR2 can down-regulate inflammation during Crohn’s disease, we have tested the efficacy of the novel agonist for FFAR2 (Cpd1) in the prevention of T1D induced by five injections of low-dose of streptozotocin in C57BL/6 mice. Oral application of Cpd1 (applied from the 1st day of T1D induction for 20 days in total) resulted in a significant reduction of T1D incidence and lowered mean glycemia levels in the treated mice. Accordingly, these mice had lower insulitis in the pancreas and higher insulin production. Ex vivo analysis on the 8th day post T1D induction showed higher proportions of innate lymphoid cell type 3 (ILC3) that produced IL-2 within the small intestine (SI) lamina propria of Cpd1-treated mice. The increase in IL-2 correlated with  higher Treg proportions detected on day 12 in the SI lamina propria. Similar findings were observed in NOD mice, a spontaneous T1D model, treated with Cpd1 on a daily basis (from 8 weeks until 12 weeks of age). Cpd1 increased the presence of ILC3 in the SI lamina propria of NOD mice, and more specifically, the proportion of Nkp46− ILC3. In total, these results demonstrate that Cpd1 increased the population of ILC3 and Treg by activating FFAR2, thereby modulating the anti-inflammatory immune response and protecting the pancreas from the imposed damage.",
publisher = "John Wiley and Sons",
journal = "7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland",
title = "Novel FFAR2 agonist prevents development of type 1 diabetes in C57BL/6 mice",
doi = "10.1002/eji.202470200",
pages = "1210"
}

Jonić, N., Koprivica, I., Mićanović, D., Saksida, T., Jevtić, B., Fraser, G. L., Miljković, Đ.,& Stojanović, I. D.. (2024). Novel FFAR2 agonist prevents development of type 1 diabetes in C57BL/6 mice. in 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland
John Wiley and Sons., 1210.
https://doi.org/10.1002/eji.202470200

Jonić N, Koprivica I, Mićanović D, Saksida T, Jevtić B, Fraser GL, Miljković Đ, Stojanović ID. Novel FFAR2 agonist prevents development of type 1 diabetes in C57BL/6 mice. in 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland. 2024;:1210.
doi:10.1002/eji.202470200 .

Jonić, Natalija, Koprivica, Ivan, Mićanović, Dragica, Saksida, Tamara, Jevtić, Bojan, Fraser, Graeme L, Miljković, Đorđe, Stojanović, Ivana D., "Novel FFAR2 agonist prevents development of type 1 diabetes in C57BL/6 mice" in 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland (2024):1210,
https://doi.org/10.1002/eji.202470200 . .

TY  - CONF
AU  - Rajaković, Mladen
AU  - Sofrenić, Ivana
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7027
AB  - Hericium erinaceus (Bull.) Persoon (fam. Hericiaceae) or Lion’s mane, widely used in traditional Chinese medicine, represents one of the most promising mushrooms with many biological activities, such as neuroprotective, neuroregenerative and antitumor. The main focus of this study was to investigate the antibacterial potential of four extracts obtained from H. erinaceus fruiting bodies using ultrasound-assisted extraction with water, ethanol, ethyl acetate and dichloromethane/methanol 2:1 (v/v) mixture. The 1H NMR profiles revealed the metabolite fingerprint of all four extracts. The highest amount of phenolic compounds, such as caffeic and ferulic acids, was detected in ethyl acetate extract, comparing the total ionic current of the detected compounds between all four extracts obtained by HPLC-Orbitrap-MS/MS. Antibacterial activity of the extracts was evaluated by microdilution method against the following pathogenic bacteria: Pseudomonas aeruginosa, resistant P. aeruginosa, Escherichia coli, Staphylococcus lugdunensis, S. aureus and methicillin-resistant S. aureus (MRSA). Compared to the other two, dichloromethane/methanol and ethyl acetate extracts possessed higher antibacterial activity against P. aeruginosa and resistant P. aeruginosa, with MIC and MBC values of 2 mg/mL and 4 mg/mL for P. aeruginosa and 3 mg/mL and 4 mg/mL for resistant P. aeruginosa. In case of E. coli, ethyl acetate extract had a MIC value of 3 mg/mL, while all extracts had a MBC value of 8 mg/mL. The obtained MIC values of 2 mg/mL for S. lugdunensis and 3 mg/mL for S. aureus and MRSA and MBC value of 4 mg/mL for S. lugdunensis, S. aureus and MRSA indicate that ethyl acetate extract was empirically validated as the most appropriate, among tested extracts, regarding the obtained antibacterial potential, and should be considered for further research. The obtained results emphasize the medicinal potential of H. erinaceus different extracts, especially considering the worldwide trend towards seeking supplements of natural origin.
PB  - Belgrade: University of Belgrade, Faculty of Agriculture
C3  - Book of abstracts: The 3rd International UNIFood Conference: UNIFood2024 Conference; 2024 Jun 28-29; Belgrade, Serbia
T1  - Chemical profiling and antibacterial activity of Hericium erinaceus extracts
SP  - 50
ER  - 

@conference{
author = "Rajaković, Mladen and Sofrenić, Ivana and Stojković, Dejan and Glamočlija, Jasmina",
year = "2024",
abstract = "Hericium erinaceus (Bull.) Persoon (fam. Hericiaceae) or Lion’s mane, widely used in traditional Chinese medicine, represents one of the most promising mushrooms with many biological activities, such as neuroprotective, neuroregenerative and antitumor. The main focus of this study was to investigate the antibacterial potential of four extracts obtained from H. erinaceus fruiting bodies using ultrasound-assisted extraction with water, ethanol, ethyl acetate and dichloromethane/methanol 2:1 (v/v) mixture. The 1H NMR profiles revealed the metabolite fingerprint of all four extracts. The highest amount of phenolic compounds, such as caffeic and ferulic acids, was detected in ethyl acetate extract, comparing the total ionic current of the detected compounds between all four extracts obtained by HPLC-Orbitrap-MS/MS. Antibacterial activity of the extracts was evaluated by microdilution method against the following pathogenic bacteria: Pseudomonas aeruginosa, resistant P. aeruginosa, Escherichia coli, Staphylococcus lugdunensis, S. aureus and methicillin-resistant S. aureus (MRSA). Compared to the other two, dichloromethane/methanol and ethyl acetate extracts possessed higher antibacterial activity against P. aeruginosa and resistant P. aeruginosa, with MIC and MBC values of 2 mg/mL and 4 mg/mL for P. aeruginosa and 3 mg/mL and 4 mg/mL for resistant P. aeruginosa. In case of E. coli, ethyl acetate extract had a MIC value of 3 mg/mL, while all extracts had a MBC value of 8 mg/mL. The obtained MIC values of 2 mg/mL for S. lugdunensis and 3 mg/mL for S. aureus and MRSA and MBC value of 4 mg/mL for S. lugdunensis, S. aureus and MRSA indicate that ethyl acetate extract was empirically validated as the most appropriate, among tested extracts, regarding the obtained antibacterial potential, and should be considered for further research. The obtained results emphasize the medicinal potential of H. erinaceus different extracts, especially considering the worldwide trend towards seeking supplements of natural origin.",
publisher = "Belgrade: University of Belgrade, Faculty of Agriculture",
journal = "Book of abstracts: The 3rd International UNIFood Conference: UNIFood2024 Conference; 2024 Jun 28-29; Belgrade, Serbia",
title = "Chemical profiling and antibacterial activity of Hericium erinaceus extracts",
pages = "50"
}

Rajaković, M., Sofrenić, I., Stojković, D.,& Glamočlija, J.. (2024). Chemical profiling and antibacterial activity of Hericium erinaceus extracts. in Book of abstracts: The 3rd International UNIFood Conference: UNIFood2024 Conference; 2024 Jun 28-29; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Agriculture., 50.

Rajaković M, Sofrenić I, Stojković D, Glamočlija J. Chemical profiling and antibacterial activity of Hericium erinaceus extracts. in Book of abstracts: The 3rd International UNIFood Conference: UNIFood2024 Conference; 2024 Jun 28-29; Belgrade, Serbia. 2024;:50..

Rajaković, Mladen, Sofrenić, Ivana, Stojković, Dejan, Glamočlija, Jasmina, "Chemical profiling and antibacterial activity of Hericium erinaceus extracts" in Book of abstracts: The 3rd International UNIFood Conference: UNIFood2024 Conference; 2024 Jun 28-29; Belgrade, Serbia (2024):50.

TY  - CONF
AU  - Jonić, Natalija
AU  - Koprivica, Ivan
AU  - Chatzigiannis, Christos
AU  - Tsiailanis, Antonis
AU  - Kyrkou, Stavroula
AU  - Tzakos, Eleftherios Paraskevas
AU  - Pavić, Aleksandar
AU  - Dimitrijević, Mirjana
AU  - Jovanović, Anđelina
AU  - Jovanović, Milan B.
AU  - Marinho, Sergio
AU  - Castro-Almeida, Ines
AU  - Otašević, Vesna
AU  - Moura-Alves, Pedro
AU  - Tzakos, Andreas
AU  - Stojanović, Ivana D.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7017
AB  - Type 1 diabetes (T1D) is a chronic autoimmune condition characterized by an imbalance between pathogenic CD4+ and
CD8+ lymphocytes on one side and regulatory T cells (Treg) on the other. Activating Treg and/or tolerogenic dendritic
cells (tolDC) through various methods has shown benefits in animal models of T1D. Both cell types express high levels
of the aryl hydrocarbon receptor (AHR), and AHR activation is typically associated with enhanced tolDC and Treg
functionality. In this study, the novel fluorescent indole-containing compound AGT-5 was investigated for its potential
to act as an AHR ligand and modulate immune cells both in vitro and in vivo. Through in silico docking analysis, AGT-
5 demonstrated the ability to bind AHR, and its agonistic effects were confirmed using the reporter Caco-2 cell line.
Additionally, due to its fluorescent properties, AGT-5 was visualized in macrophages in vitro and in the small intestine
lamina propria ex vivo after oral administration using confocal microscopy. AGT-5 exhibited no toxicity towards murine
macrophages and human tonsillar cells. Moreover, tests on zebrafish embryos revealed no nephrotoxicity, hepatotoxicity,
or cardiotoxicity. Utilizing an ADME virtual platform it was confirmed that AGT-5 possesses drug-like characteristics.
Subsequently, AGT-5 was orally administered to C57BL/6 mice that had received low doses of streptozotocin to induce
T1D. Treatment with AGT-5 commencing from the first day of T1D induction and lasting for 20 days effectively
prevented immune cell infiltration into the pancreas, preserved insulin production, and halted the progression of T1D.
AGT-5 achieved this by promoting tolDC and Treg activity along the gut-pancreatic lymph node-pancreas axis.
Mechanistically, AGT-5 upregulated indoleamine 2,3-dioxygenase 1 in tolDC and enhanced ATP-degrading enzyme
expression on Treg, thereby promoting immunosuppressive action. The positive outcomes seen in T1D animals suggest
that AGT-5 holds promise for a potential treatment for inflammatory conditions that can benefit from stimulation of the
regulatory arm of the immune response.
PB  - John Wiley and Sons
C3  - 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland
T1  - A novel aryl hydrocarbon receptor ligand inhibits the development of Type 1 diabetes by enhancing the function of tolerogenic dendritic cells and regulatory T cells
IS  - 54
DO  - 10.1002/eji.202470200
SP  - 372
EP  - 373
ER  - 

@conference{
author = "Jonić, Natalija and Koprivica, Ivan and Chatzigiannis, Christos and Tsiailanis, Antonis and Kyrkou, Stavroula and Tzakos, Eleftherios Paraskevas and Pavić, Aleksandar and Dimitrijević, Mirjana and Jovanović, Anđelina and Jovanović, Milan B. and Marinho, Sergio and Castro-Almeida, Ines and Otašević, Vesna and Moura-Alves, Pedro and Tzakos, Andreas and Stojanović, Ivana D.",
year = "2024",
abstract = "Type 1 diabetes (T1D) is a chronic autoimmune condition characterized by an imbalance between pathogenic CD4+ and
CD8+ lymphocytes on one side and regulatory T cells (Treg) on the other. Activating Treg and/or tolerogenic dendritic
cells (tolDC) through various methods has shown benefits in animal models of T1D. Both cell types express high levels
of the aryl hydrocarbon receptor (AHR), and AHR activation is typically associated with enhanced tolDC and Treg
functionality. In this study, the novel fluorescent indole-containing compound AGT-5 was investigated for its potential
to act as an AHR ligand and modulate immune cells both in vitro and in vivo. Through in silico docking analysis, AGT-
5 demonstrated the ability to bind AHR, and its agonistic effects were confirmed using the reporter Caco-2 cell line.
Additionally, due to its fluorescent properties, AGT-5 was visualized in macrophages in vitro and in the small intestine
lamina propria ex vivo after oral administration using confocal microscopy. AGT-5 exhibited no toxicity towards murine
macrophages and human tonsillar cells. Moreover, tests on zebrafish embryos revealed no nephrotoxicity, hepatotoxicity,
or cardiotoxicity. Utilizing an ADME virtual platform it was confirmed that AGT-5 possesses drug-like characteristics.
Subsequently, AGT-5 was orally administered to C57BL/6 mice that had received low doses of streptozotocin to induce
T1D. Treatment with AGT-5 commencing from the first day of T1D induction and lasting for 20 days effectively
prevented immune cell infiltration into the pancreas, preserved insulin production, and halted the progression of T1D.
AGT-5 achieved this by promoting tolDC and Treg activity along the gut-pancreatic lymph node-pancreas axis.
Mechanistically, AGT-5 upregulated indoleamine 2,3-dioxygenase 1 in tolDC and enhanced ATP-degrading enzyme
expression on Treg, thereby promoting immunosuppressive action. The positive outcomes seen in T1D animals suggest
that AGT-5 holds promise for a potential treatment for inflammatory conditions that can benefit from stimulation of the
regulatory arm of the immune response.",
publisher = "John Wiley and Sons",
journal = "7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland",
title = "A novel aryl hydrocarbon receptor ligand inhibits the development of Type 1 diabetes by enhancing the function of tolerogenic dendritic cells and regulatory T cells",
number = "54",
doi = "10.1002/eji.202470200",
pages = "372-373"
}

Jonić, N., Koprivica, I., Chatzigiannis, C., Tsiailanis, A., Kyrkou, S., Tzakos, E. P., Pavić, A., Dimitrijević, M., Jovanović, A., Jovanović, M. B., Marinho, S., Castro-Almeida, I., Otašević, V., Moura-Alves, P., Tzakos, A.,& Stojanović, I. D.. (2024). A novel aryl hydrocarbon receptor ligand inhibits the development of Type 1 diabetes by enhancing the function of tolerogenic dendritic cells and regulatory T cells. in 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland
John Wiley and Sons.(54), 372-373.
https://doi.org/10.1002/eji.202470200

Jonić N, Koprivica I, Chatzigiannis C, Tsiailanis A, Kyrkou S, Tzakos EP, Pavić A, Dimitrijević M, Jovanović A, Jovanović MB, Marinho S, Castro-Almeida I, Otašević V, Moura-Alves P, Tzakos A, Stojanović ID. A novel aryl hydrocarbon receptor ligand inhibits the development of Type 1 diabetes by enhancing the function of tolerogenic dendritic cells and regulatory T cells. in 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland. 2024;(54):372-373.
doi:10.1002/eji.202470200 .

Jonić, Natalija, Koprivica, Ivan, Chatzigiannis, Christos, Tsiailanis, Antonis, Kyrkou, Stavroula, Tzakos, Eleftherios Paraskevas, Pavić, Aleksandar, Dimitrijević, Mirjana, Jovanović, Anđelina, Jovanović, Milan B., Marinho, Sergio, Castro-Almeida, Ines, Otašević, Vesna, Moura-Alves, Pedro, Tzakos, Andreas, Stojanović, Ivana D., "A novel aryl hydrocarbon receptor ligand inhibits the development of Type 1 diabetes by enhancing the function of tolerogenic dendritic cells and regulatory T cells" in 7th European Congress of Immunology: ECI 2024; 2024 Sep 1-4; Dublin, Ireland, no. 54 (2024):372-373,
https://doi.org/10.1002/eji.202470200 . .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Marin, Marija
AU  - Maksimović, Vuk
AU  - Trajković, Milena
AU  - Stanišić, Mariana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7016
AB  - Phloretin is a phenolic compound from apple trees that is also found in traces in some other plant
species such as Pieris japonica, Hoveniae lignum and Loiseleuria procumbens. The role of
phloretin in plants is less known, although it is widely used in pharmaceuticals and cosmetics due
to its excellent antioxidant properties, anti-cancer and immunoprotective effects. The autotoxicity
of apple trees is known as Apple Replant Disease (ARD), but knowledge about phloretin effect on
other plant species is very scarce. Our research team was the first to report on the phytotoxicity of
apple hairy root exudates1. Our current research is based on the discovery of the molecular and
physiological pathways of phloretin allelopathic activity in the model plant Arabidopsis thaliana
(L.) Heynh. Our studies show that the inhibitory effect of phloretin manifests at different levels
with rapid and cumulative effects. Phloretin most likely affects the plant in several ways: on the
one hand, it influences the metabolism and distribution of auxin2 and, on the other hand, affects
photosynthesis. The intensive glycosylation of phloretin in A.thaliana tissues is one of the earliest
activity of plant defense strategies. However, it is insufficient to prevent phloretin early phytotoxic
effects that include elevated expression of genes involved in polar auxin transport and auxin
accumulation in lateral parts of the root tips, leading to the inhibition of root growth, and impaired
gravitropic response. Further exposure to phloretin results in the relocation and swelling of
chloroplasts, changes of thylakoid organization and arrest of photosynthesis, all together leading
to the depletion of starch reserves, cell starvation, and recruitment of self-preservation micro- and
macroautophagic processes resulting in programmed cell death. We propose that the interplay
between auxin signaling and cell starvation is a key to the phytotoxic action of phloretin in model
plant A.thaliana.
PB  - International Allelopathy Society
C3  - Book of abstracts: IAS 9th World Congress on Allelopathy: Allelopathy in a changing world; 2024 Jul 8-10; Caserta, Italy
T1  - Phloretin – A Lesser-Known Allelochemical from the Apple Trees and Its Proposed Mode of Action
SP  - 14
ER  - 

@conference{
author = "Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Marin, Marija and Maksimović, Vuk and Trajković, Milena and Stanišić, Mariana",
year = "2024",
abstract = "Phloretin is a phenolic compound from apple trees that is also found in traces in some other plant
species such as Pieris japonica, Hoveniae lignum and Loiseleuria procumbens. The role of
phloretin in plants is less known, although it is widely used in pharmaceuticals and cosmetics due
to its excellent antioxidant properties, anti-cancer and immunoprotective effects. The autotoxicity
of apple trees is known as Apple Replant Disease (ARD), but knowledge about phloretin effect on
other plant species is very scarce. Our research team was the first to report on the phytotoxicity of
apple hairy root exudates1. Our current research is based on the discovery of the molecular and
physiological pathways of phloretin allelopathic activity in the model plant Arabidopsis thaliana
(L.) Heynh. Our studies show that the inhibitory effect of phloretin manifests at different levels
with rapid and cumulative effects. Phloretin most likely affects the plant in several ways: on the
one hand, it influences the metabolism and distribution of auxin2 and, on the other hand, affects
photosynthesis. The intensive glycosylation of phloretin in A.thaliana tissues is one of the earliest
activity of plant defense strategies. However, it is insufficient to prevent phloretin early phytotoxic
effects that include elevated expression of genes involved in polar auxin transport and auxin
accumulation in lateral parts of the root tips, leading to the inhibition of root growth, and impaired
gravitropic response. Further exposure to phloretin results in the relocation and swelling of
chloroplasts, changes of thylakoid organization and arrest of photosynthesis, all together leading
to the depletion of starch reserves, cell starvation, and recruitment of self-preservation micro- and
macroautophagic processes resulting in programmed cell death. We propose that the interplay
between auxin signaling and cell starvation is a key to the phytotoxic action of phloretin in model
plant A.thaliana.",
publisher = "International Allelopathy Society",
journal = "Book of abstracts: IAS 9th World Congress on Allelopathy: Allelopathy in a changing world; 2024 Jul 8-10; Caserta, Italy",
title = "Phloretin – A Lesser-Known Allelochemical from the Apple Trees and Its Proposed Mode of Action",
pages = "14"
}

Smailagić, D., Banjac, N., Ninković, S., Marin, M., Maksimović, V., Trajković, M.,& Stanišić, M.. (2024). Phloretin – A Lesser-Known Allelochemical from the Apple Trees and Its Proposed Mode of Action. in Book of abstracts: IAS 9th World Congress on Allelopathy: Allelopathy in a changing world; 2024 Jul 8-10; Caserta, Italy
International Allelopathy Society., 14.

Smailagić D, Banjac N, Ninković S, Marin M, Maksimović V, Trajković M, Stanišić M. Phloretin – A Lesser-Known Allelochemical from the Apple Trees and Its Proposed Mode of Action. in Book of abstracts: IAS 9th World Congress on Allelopathy: Allelopathy in a changing world; 2024 Jul 8-10; Caserta, Italy. 2024;:14..

Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Marin, Marija, Maksimović, Vuk, Trajković, Milena, Stanišić, Mariana, "Phloretin – A Lesser-Known Allelochemical from the Apple Trees and Its Proposed Mode of Action" in Book of abstracts: IAS 9th World Congress on Allelopathy: Allelopathy in a changing world; 2024 Jul 8-10; Caserta, Italy (2024):14.

TY  - CONF
AU  - Janković, Zorica
AU  - Radisavljević, Ljiljana
AU  - Nježić, Irena
AU  - Vučkovac, Obrad
AU  - Ševkušić, Milica
PY  - 2024
UR  - https://pubmet2024.unizd.hr/wp-content/uploads/2024/09/BoA_PUBMET2024.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7015
AB  - Open science promotes transparency, open access, and reproducibility in research, ensuring that research outputs are openly available and reusable by others. However, traditional research assessment systems usually emphasize quantitative indicators, such as journal impact factors and citation counts, encouraging publication in ‘high-prestige’ journals and failing to fully capture the value of open science practices. Accordingly, a Current Research Information System (CRIS) tracking research outputs for assessment purposes does not necessarily encourage open science practices. This presentation explores the challenges and strategies involved in reconciling open science practices with research assessment requirements in Serbia during the process of establishing the national CRIS system eNauka. Over the past decade, the Serbian research community has made significant efforts to implement the principles of open science. This process has intensified since 2018, following the adoption of the Open Science Platform, which introduced a green open mandate, leading to the development of more than 80 institutional repositories within five years, most of which ensure compliance with the FAIR principles and are harvested by international aggregators. Research organizations have recognized the role of repositories in preserving research results and ensuring wide dissemination and improved visibility through open access. Intensive informal training provided by librarians and community development (Open Science Community, 2021) have been instrumental in raising awareness and improving the understanding of open science practices among researchers. In 2022, the ministry responsible for science started developing an information system to track research outputs in Serbia for reporting and assessment purposes, eNauka (eScience) portal. The portal provides registries of all accredited research organizations and all researchers in Serbia, as well as a database containing metadata about their research outputs, which predominantly include publications, patents and technical reports. Administrative data about organizations and researchers are curated by the organizations’ administrative staff and the ministry personnel. Metadata describing research outputs are harvested (e.g. Cobiss OPAC) or imported (Crossref, ORCID profiles) from various sources, but institutional repositories are the main data source. Metadata records in eNauka are harvested once a week and curated by officially appointed individuals from research organizations, usually librarians and repository managers. Thanks to this, the establishment of the national CRIS system additionally encouraged the development of institutional repositories. However, this process has been accompanied with some challenges and has threatened to disrupt progress in adopting open science practices. Here is a brief overview of the major challenges: One of the functions of eNauka is to facilitate research assessment. Along with publications, patents, technical reports, artworks and similar outputs that can be deposited in a repository, the national regulations recognize a number of results the evidence of which (e.g. signed certificates, invitation letters, diplomas) is typically not deposited in a repository. Due to this, repository managers have faced pressure to allow depositing materials not allowed by repository policies. A number of research organizations established repositories with the sole idea of feeding data into the national CRIS system. In such cases, repositories provided only metadata and it was practically impossible to persuade researchers to deposit full-text content, let alone to make it open access. In a number of well-functioning repositories good depositing practices were disrupted, as a number of researchers refused to provide full-text content. They either only provided metadata or deposited the front pages of their publications and insisted on depositing inappropriate materials. A major challenge was the pressure to align institutional repositories, using standard metadata schemas and vocabularies, with the non-standard classifications used by the ministry. The initiative to deal with these challenges came from the library community and the ministry accepted the request to establish a working group to develop guidelines for metadata curation in eNauka. It included librarians, i.e. repository managers, ministry analysts, the administrators of the eNauka portal, and ministry representatives. Their task was to harmonize the requirements of all stakeholders towards facilitating research assessment while maintaining the integrity of repositories and fostering open science practices. The working group produced two sets of guidelines (adopted in March 2024): guidelines for metadata curation in eNauka  explaining how to describe research outputs using standardized metadata in eNauka, and guidelines for repository managers, detailing the metadata and providing clear instructions regarding full-text deposition. These guidelines make it possible to meet the assessment requirements, while empowering the managers of institutional repositories to spread awareness at the level of their organization about the multiple benefits of open science. In this respect, the guidelines are particularly valuable for newly established repositories. The fact that the national CRIS system relies on repository data opens up many possibilities for the recognition and tracking of open research practices in research assessment. However, there are still many challenges to overcome, such as the lack of awareness and understanding of open science practices among researchers, ministry analysts and national policy makers. Many researchers are not fully aware of the benefits of open science or how to effectively implement open practices in their work. Similarly, ministry analysts lack the knowledge and tools needed to evaluate the full spectrum of research outputs that open science encompasses. Cultural resistance within the academic community also poses a barrier. Established norms and practices in research and assessment are deeply ingrained, and resistance to change is widespread in the local research community. Researchers are hesitant to embrace open science practices if they perceive them as risky, believe that these practices might negatively impact their career prospects, or simply if they get no reward for them. Policy reforms are crucial in integrating open science practices into research assessment. Funding agencies, universities, and research institutions should adopt policies that recognize and reward open science contributions.
PB  - Zadar: University of Zadar
C3  - Book of abstracts: The 11th Conference on Scholarly Communication in the context of Open Science: PUBMET2024; 2024 Sep 12-13; Zadar, Croatia
T1  - Reconciling open science practices and research assessment requirements in the process of establishing a national CRIS system in Serbia
SP  - 31
EP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_7015
ER  - 

@conference{
author = "Janković, Zorica and Radisavljević, Ljiljana and Nježić, Irena and Vučkovac, Obrad and Ševkušić, Milica",
year = "2024",
abstract = "Open science promotes transparency, open access, and reproducibility in research, ensuring that research outputs are openly available and reusable by others. However, traditional research assessment systems usually emphasize quantitative indicators, such as journal impact factors and citation counts, encouraging publication in ‘high-prestige’ journals and failing to fully capture the value of open science practices. Accordingly, a Current Research Information System (CRIS) tracking research outputs for assessment purposes does not necessarily encourage open science practices. This presentation explores the challenges and strategies involved in reconciling open science practices with research assessment requirements in Serbia during the process of establishing the national CRIS system eNauka. Over the past decade, the Serbian research community has made significant efforts to implement the principles of open science. This process has intensified since 2018, following the adoption of the Open Science Platform, which introduced a green open mandate, leading to the development of more than 80 institutional repositories within five years, most of which ensure compliance with the FAIR principles and are harvested by international aggregators. Research organizations have recognized the role of repositories in preserving research results and ensuring wide dissemination and improved visibility through open access. Intensive informal training provided by librarians and community development (Open Science Community, 2021) have been instrumental in raising awareness and improving the understanding of open science practices among researchers. In 2022, the ministry responsible for science started developing an information system to track research outputs in Serbia for reporting and assessment purposes, eNauka (eScience) portal. The portal provides registries of all accredited research organizations and all researchers in Serbia, as well as a database containing metadata about their research outputs, which predominantly include publications, patents and technical reports. Administrative data about organizations and researchers are curated by the organizations’ administrative staff and the ministry personnel. Metadata describing research outputs are harvested (e.g. Cobiss OPAC) or imported (Crossref, ORCID profiles) from various sources, but institutional repositories are the main data source. Metadata records in eNauka are harvested once a week and curated by officially appointed individuals from research organizations, usually librarians and repository managers. Thanks to this, the establishment of the national CRIS system additionally encouraged the development of institutional repositories. However, this process has been accompanied with some challenges and has threatened to disrupt progress in adopting open science practices. Here is a brief overview of the major challenges: One of the functions of eNauka is to facilitate research assessment. Along with publications, patents, technical reports, artworks and similar outputs that can be deposited in a repository, the national regulations recognize a number of results the evidence of which (e.g. signed certificates, invitation letters, diplomas) is typically not deposited in a repository. Due to this, repository managers have faced pressure to allow depositing materials not allowed by repository policies. A number of research organizations established repositories with the sole idea of feeding data into the national CRIS system. In such cases, repositories provided only metadata and it was practically impossible to persuade researchers to deposit full-text content, let alone to make it open access. In a number of well-functioning repositories good depositing practices were disrupted, as a number of researchers refused to provide full-text content. They either only provided metadata or deposited the front pages of their publications and insisted on depositing inappropriate materials. A major challenge was the pressure to align institutional repositories, using standard metadata schemas and vocabularies, with the non-standard classifications used by the ministry. The initiative to deal with these challenges came from the library community and the ministry accepted the request to establish a working group to develop guidelines for metadata curation in eNauka. It included librarians, i.e. repository managers, ministry analysts, the administrators of the eNauka portal, and ministry representatives. Their task was to harmonize the requirements of all stakeholders towards facilitating research assessment while maintaining the integrity of repositories and fostering open science practices. The working group produced two sets of guidelines (adopted in March 2024): guidelines for metadata curation in eNauka  explaining how to describe research outputs using standardized metadata in eNauka, and guidelines for repository managers, detailing the metadata and providing clear instructions regarding full-text deposition. These guidelines make it possible to meet the assessment requirements, while empowering the managers of institutional repositories to spread awareness at the level of their organization about the multiple benefits of open science. In this respect, the guidelines are particularly valuable for newly established repositories. The fact that the national CRIS system relies on repository data opens up many possibilities for the recognition and tracking of open research practices in research assessment. However, there are still many challenges to overcome, such as the lack of awareness and understanding of open science practices among researchers, ministry analysts and national policy makers. Many researchers are not fully aware of the benefits of open science or how to effectively implement open practices in their work. Similarly, ministry analysts lack the knowledge and tools needed to evaluate the full spectrum of research outputs that open science encompasses. Cultural resistance within the academic community also poses a barrier. Established norms and practices in research and assessment are deeply ingrained, and resistance to change is widespread in the local research community. Researchers are hesitant to embrace open science practices if they perceive them as risky, believe that these practices might negatively impact their career prospects, or simply if they get no reward for them. Policy reforms are crucial in integrating open science practices into research assessment. Funding agencies, universities, and research institutions should adopt policies that recognize and reward open science contributions.",
publisher = "Zadar: University of Zadar",
journal = "Book of abstracts: The 11th Conference on Scholarly Communication in the context of Open Science: PUBMET2024; 2024 Sep 12-13; Zadar, Croatia",
title = "Reconciling open science practices and research assessment requirements in the process of establishing a national CRIS system in Serbia",
pages = "31-34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7015"
}

Janković, Z., Radisavljević, L., Nježić, I., Vučkovac, O.,& Ševkušić, M.. (2024). Reconciling open science practices and research assessment requirements in the process of establishing a national CRIS system in Serbia. in Book of abstracts: The 11th Conference on Scholarly Communication in the context of Open Science: PUBMET2024; 2024 Sep 12-13; Zadar, Croatia
Zadar: University of Zadar., 31-34.
https://hdl.handle.net/21.15107/rcub_ibiss_7015

Janković Z, Radisavljević L, Nježić I, Vučkovac O, Ševkušić M. Reconciling open science practices and research assessment requirements in the process of establishing a national CRIS system in Serbia. in Book of abstracts: The 11th Conference on Scholarly Communication in the context of Open Science: PUBMET2024; 2024 Sep 12-13; Zadar, Croatia. 2024;:31-34.
https://hdl.handle.net/21.15107/rcub_ibiss_7015 .

Janković, Zorica, Radisavljević, Ljiljana, Nježić, Irena, Vučkovac, Obrad, Ševkušić, Milica, "Reconciling open science practices and research assessment requirements in the process of establishing a national CRIS system in Serbia" in Book of abstracts: The 11th Conference on Scholarly Communication in the context of Open Science: PUBMET2024; 2024 Sep 12-13; Zadar, Croatia (2024):31-34,
https://hdl.handle.net/21.15107/rcub_ibiss_7015 .

TY  - CONF
AU  - Vukajlović, Filip
AU  - Savković, Uroš
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Vlajnić, Lea
AU  - Đorđević, Mirko
PY  - 2024
UR  - https://www.royensoc.co.uk/event/ento24-in-person/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7011
AB  - The seed beetle Acanthoscelides obtectus is one of the most economically important pests of stored legume seeds worldwide and is traditionally controlled with synthetic pesticides. However, the frequent use of these toxic compounds leads to environmental and health damage and the evolution of resistant insect populations. The Trojan Female Technique (TFT) is a novel species-specific, transgenerational biocontrol method for pest management. Sustained population control is achieved by releasing of Trojan females (TF) that carry naturally occurring mitochondrial DNA mutations (TFT mutation) that impair male fertility, but have no effects on females. TF and their female offspring could continuously, over multiple generations, produce males that sire fewer offspring than their wild-type counterparts. A candidate TFT mutation that causes male-only subfertility was recently described in A. obtectus. However, the applicability of TFT depends on mitochondrial TFT mutations whose male-sterilizing effects are general across different nuclear genomic contexts. We tested this assumption by expressing the candidate TFT mitochondrial haplotype alongside a range of three nuclear backgrounds and comparing its fertility in males with that of control haplotypes. We found that the fertility of males harbouring the candidate TFT mutation is consistently lower in all three nuclear backgrounds.
PB  - Royal Entomological Society
C3  - Ento24: Annual conference; 2024 Sep 10-12; Liverpool, United Kingdom
T1  - Trojan Female Technique for the biocontrol of the seed beetle Acanthoscelides obtectus: mitochondrial haplotype has negative male-specific effect on fertility across different nuclear backgrounds
ER  - 

@conference{
author = "Vukajlović, Filip and Savković, Uroš and Budečević, Sanja and Stojković, Biljana and Vlajnić, Lea and Đorđević, Mirko",
year = "2024",
abstract = "The seed beetle Acanthoscelides obtectus is one of the most economically important pests of stored legume seeds worldwide and is traditionally controlled with synthetic pesticides. However, the frequent use of these toxic compounds leads to environmental and health damage and the evolution of resistant insect populations. The Trojan Female Technique (TFT) is a novel species-specific, transgenerational biocontrol method for pest management. Sustained population control is achieved by releasing of Trojan females (TF) that carry naturally occurring mitochondrial DNA mutations (TFT mutation) that impair male fertility, but have no effects on females. TF and their female offspring could continuously, over multiple generations, produce males that sire fewer offspring than their wild-type counterparts. A candidate TFT mutation that causes male-only subfertility was recently described in A. obtectus. However, the applicability of TFT depends on mitochondrial TFT mutations whose male-sterilizing effects are general across different nuclear genomic contexts. We tested this assumption by expressing the candidate TFT mitochondrial haplotype alongside a range of three nuclear backgrounds and comparing its fertility in males with that of control haplotypes. We found that the fertility of males harbouring the candidate TFT mutation is consistently lower in all three nuclear backgrounds.",
publisher = "Royal Entomological Society",
journal = "Ento24: Annual conference; 2024 Sep 10-12; Liverpool, United Kingdom",
title = "Trojan Female Technique for the biocontrol of the seed beetle Acanthoscelides obtectus: mitochondrial haplotype has negative male-specific effect on fertility across different nuclear backgrounds"
}

Vukajlović, F., Savković, U., Budečević, S., Stojković, B., Vlajnić, L.,& Đorđević, M.. (2024). Trojan Female Technique for the biocontrol of the seed beetle Acanthoscelides obtectus: mitochondrial haplotype has negative male-specific effect on fertility across different nuclear backgrounds. in Ento24: Annual conference; 2024 Sep 10-12; Liverpool, United Kingdom
Royal Entomological Society..

Vukajlović F, Savković U, Budečević S, Stojković B, Vlajnić L, Đorđević M. Trojan Female Technique for the biocontrol of the seed beetle Acanthoscelides obtectus: mitochondrial haplotype has negative male-specific effect on fertility across different nuclear backgrounds. in Ento24: Annual conference; 2024 Sep 10-12; Liverpool, United Kingdom. 2024;..

Vukajlović, Filip, Savković, Uroš, Budečević, Sanja, Stojković, Biljana, Vlajnić, Lea, Đorđević, Mirko, "Trojan Female Technique for the biocontrol of the seed beetle Acanthoscelides obtectus: mitochondrial haplotype has negative male-specific effect on fertility across different nuclear backgrounds" in Ento24: Annual conference; 2024 Sep 10-12; Liverpool, United Kingdom (2024).

TY  - CONF
AU  - Savković, Uroš
AU  - Budečević, Sanja
AU  - Pešić, Snežana
AU  - Predojević, Dragana
AU  - Vukajlović, Filip
AU  - Vlajnić, Lea
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7010
UR  - https://www.royensoc.co.uk/event/ento24-in-person/
AB  - Many phytophagous insects are able to exploit a novel food source in their diets. This process, known as the host shift, includes insects’ ability to recognise, utilise and ultimately persist on a new plant host. During the host shift process, insects are faced with many challenges and new conditions can change insects’ behaviour, physiology, life-history strategies and population dynamics. Adaptive phenotypic plasticity could help populations to receive signals from the new environment and constitute a functional phenotype. Using a long-term laboratory evolution experiment in seed beetles (Acanthoscelides obtectus) we have tested how different plant hosts (common beans, chickpeas and mung beans) change life-history strategies over many generations. Furthermore, we have established iso-female lines and conducted a reciprocal transplant experiment in order to test the level of plasticity in life-history traits. We have demonstrated that populations of seed beetles adapted to different hosts not only show changes in life-history traits, but also demonstrate clear differences in the levels of phenotypic plasticity for those traits. This research is discussed in the context of how plant hosts affect populations’ ability to influence long‐term (evolutionary) modes of populations’ change and allow survival and ecological competence of organisms in new conditions.
PB  - Royal Entomological Society
C3  - Ento24: Annual conference; 2024 Sep 10-12; Liverpool, United Kingdom
T1  - Long-term laboratory evolution experiment: Host shift changes the life-history strategies and the levels of phenotypic plasticity in seed beetle (Acanthoscelides obtectus)
ER  - 

@conference{
author = "Savković, Uroš and Budečević, Sanja and Pešić, Snežana and Predojević, Dragana and Vukajlović, Filip and Vlajnić, Lea and Đorđević, Mirko and Stojković, Biljana",
year = "2024",
abstract = "Many phytophagous insects are able to exploit a novel food source in their diets. This process, known as the host shift, includes insects’ ability to recognise, utilise and ultimately persist on a new plant host. During the host shift process, insects are faced with many challenges and new conditions can change insects’ behaviour, physiology, life-history strategies and population dynamics. Adaptive phenotypic plasticity could help populations to receive signals from the new environment and constitute a functional phenotype. Using a long-term laboratory evolution experiment in seed beetles (Acanthoscelides obtectus) we have tested how different plant hosts (common beans, chickpeas and mung beans) change life-history strategies over many generations. Furthermore, we have established iso-female lines and conducted a reciprocal transplant experiment in order to test the level of plasticity in life-history traits. We have demonstrated that populations of seed beetles adapted to different hosts not only show changes in life-history traits, but also demonstrate clear differences in the levels of phenotypic plasticity for those traits. This research is discussed in the context of how plant hosts affect populations’ ability to influence long‐term (evolutionary) modes of populations’ change and allow survival and ecological competence of organisms in new conditions.",
publisher = "Royal Entomological Society",
journal = "Ento24: Annual conference; 2024 Sep 10-12; Liverpool, United Kingdom",
title = "Long-term laboratory evolution experiment: Host shift changes the life-history strategies and the levels of phenotypic plasticity in seed beetle (Acanthoscelides obtectus)"
}

Savković, U., Budečević, S., Pešić, S., Predojević, D., Vukajlović, F., Vlajnić, L., Đorđević, M.,& Stojković, B.. (2024). Long-term laboratory evolution experiment: Host shift changes the life-history strategies and the levels of phenotypic plasticity in seed beetle (Acanthoscelides obtectus). in Ento24: Annual conference; 2024 Sep 10-12; Liverpool, United Kingdom
Royal Entomological Society..

Savković U, Budečević S, Pešić S, Predojević D, Vukajlović F, Vlajnić L, Đorđević M, Stojković B. Long-term laboratory evolution experiment: Host shift changes the life-history strategies and the levels of phenotypic plasticity in seed beetle (Acanthoscelides obtectus). in Ento24: Annual conference; 2024 Sep 10-12; Liverpool, United Kingdom. 2024;..

Savković, Uroš, Budečević, Sanja, Pešić, Snežana, Predojević, Dragana, Vukajlović, Filip, Vlajnić, Lea, Đorđević, Mirko, Stojković, Biljana, "Long-term laboratory evolution experiment: Host shift changes the life-history strategies and the levels of phenotypic plasticity in seed beetle (Acanthoscelides obtectus)" in Ento24: Annual conference; 2024 Sep 10-12; Liverpool, United Kingdom (2024).

TY  - CONF
AU  - Đorđević, Ana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7006
AB  - Гојазност представља један од водећих здравствених проблема савременог доба, и често је праћена поремећајима као што су дислипидемија, инсулинска резистенција и атеросклероза. Иако се у основи гојазности налазе и генетички и средински фактори, њен најчешћи узрочник је савремени начин живота, који подразумева исхрану са пуно шећера и масти. Контролисане студије на анималним моделима гојазности изазване исхраном од великог су значаја за проучавање механизама њеног настанка, али и за унапређење постојећих и развој нових терапија.
У нашим истраживањима користили смо анималне моделе гојазности на глодарима, и то пацове храњене са 60% фруктозе у периоду од девет недеља и мишеве који су 14 недеља били на исхрани са 60% масти. У оба модела забележен је повећан енергетски унос, с тим што исхрана обогаћена фруктозом није довела до повећања телесне масе, али је утицала на повећање масе висцералног масног ткива. За разлику од фруктозе, исхрана са високим садржајем масти довела је до увећања укупне телесне масе и масе висцералног масног ткива мишева. Код ових животиња били су присутни и знаци системске инсулинске резистенције, која је изостала на моделу пацова храњених фруктозом. Са друге стране, исхрана обогаћена фруктозом довела је до хипертриглицеридемије, али без појаве карактеристика масне јетре, док су мишеви на масној исхрани имали изражену стеатозу.
Оба испитивана анимална модела могу бити корисна за ендокриноло- шка истраживања, с тим што анимални модел гојазности индуковане масном храном показује значајнију сличност са карактеристикама гојазности код људи, док је исхрана обогаћена фруктозом бољи анимални
модел за метаболичке промене као што су дислипидемија и адипогенеза.
AB  - Obesity is one of today’s major health problems and is often associated with chronic
diseases such as dyslipidemia, insulin resistance and atherosclerosis. Although both
genetic and environmental factors can be the cause of obesity, the most common cause
is the modern lifestyle, which includes a hypercaloric diet high in sugar and fat. Controlled
studies in animal models of diet-induced obesity are of great importance for the
investigation of the mechanisms of its development, but also for the improvement of
existing and the development of new therapies.
In our research, we used animal models of obesity in rodents, namely a rat fed a
60% fructose solution in water for 9 weeks and a mouse fed a 60% fat diet for 14 weeks.
Increased energy intake was observed in both animal models, although a high
fructose diet did not lead to an increase in body weight but did lead to an increase in
visceral adipose tissue (VAT) mass. In contrast to fructose, a high-fat diet resulted in
the increase of body and VAT mass. These animals also showed signs of systemic insulin
resistance, which was not the case in the fructose-fed rat model. On the other hand, a
fructose-enriched diet resulted in hypertriglyceridemia, but without the appearance of
fatty liver, while mice on a high-fat diet showed marked steatosis.
Both animal models may be useful for endocrinology research, with the high-fat
diet animal model having greater similarity to features of human obesity, while the
fructose-enriched diet is a better animal model for metabolic changes such as dyslipidemia
and adipogenesis.
AB  - Gojaznost predstavlja jedan od vodećih zdravstvenih problema savremenog doba, i često je praćena poremećajima kao što su dislipidemija, insulinska rezistencija i ateroskleroza. Iako se u osnovi gojaznosti nalaze i genetički i sredinski faktori, njen najčešći uzročnik je savremeni način života, koji podrazumeva ishranu sa puno šećera i masti. Kontrolisane studije na animalnim modelima gojaznosti izazvane ishranom od velikog su značaja za proučavanje mehanizama njenog nastanka, ali i za unapređenje postojećih i razvoj novih terapija. U našim istraživanjima koristili smo animalne modele gojaznosti na glodarima, i to pacove hranjene sa 60% fruktoze u periodu od devet nedelja i miševe koji su 14 nedelja bili na ishrani sa 60% masti. U oba modela zabeležen je povećan energetski unos, s tim što ishrana obogaćena fruktozom nije dovela do povećanja telesne mase, ali je uticala na povećanje mase visceralnog masnog tkiva. Za razliku od fruktoze, ishrana sa visokim sadržajem masti dovela je do uvećanja ukupne telesne mase i mase visceralnog masnog tkiva miševa. Kod ovih životinja bili su prisutni i znaci sistemske insulinske rezistencije, koja je izostala na modelu pacova hranjenih fruktozom. Sa druge strane, ishrana obogaćena fruktozom dovela je do hipertrigliceridemije, ali bez pojave karakteristika masne jetre, dok su miševi na masnoj ishrani imali izraženu steatozu. Oba ispitivana animalna modela mogu biti korisna za endokrinolo- ška istraživanja, s tim što animalni model gojaznosti indukovane masnom hranom pokazuje značajniju sličnost sa karakteristikama gojaznosti kod ljudi, dok je ishrana obogaćena fruktozom bolji animalni model za metaboličke promene kao što su dislipidemija i adipogeneza.
PB  - Beograd: Srpska akademija nauka i umetnosti
C3  - Novine u gojaznosti 2023: Zbornik radova sa naučnog skupa održanog 9. novembra 2023. godine; 2023 nov 9; Belgrade, Serbia
T1  - Karakteristike animalnih modela gojaznosti indukovanih različitim tipovima ishrane
T1  - Карактеристике анималних модела гојазности индукованих различитим типовима исхране
T1  - Characteristics of animal models of obesity induced by different food types
SP  - 75
EP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_7006
ER  - 

@conference{
author = "Đorđević, Ana",
year = "2024",
abstract = "Гојазност представља један од водећих здравствених проблема савременог доба, и често је праћена поремећајима као што су дислипидемија, инсулинска резистенција и атеросклероза. Иако се у основи гојазности налазе и генетички и средински фактори, њен најчешћи узрочник је савремени начин живота, који подразумева исхрану са пуно шећера и масти. Контролисане студије на анималним моделима гојазности изазване исхраном од великог су значаја за проучавање механизама њеног настанка, али и за унапређење постојећих и развој нових терапија.
У нашим истраживањима користили смо анималне моделе гојазности на глодарима, и то пацове храњене са 60% фруктозе у периоду од девет недеља и мишеве који су 14 недеља били на исхрани са 60% масти. У оба модела забележен је повећан енергетски унос, с тим што исхрана обогаћена фруктозом није довела до повећања телесне масе, али је утицала на повећање масе висцералног масног ткива. За разлику од фруктозе, исхрана са високим садржајем масти довела је до увећања укупне телесне масе и масе висцералног масног ткива мишева. Код ових животиња били су присутни и знаци системске инсулинске резистенције, која је изостала на моделу пацова храњених фруктозом. Са друге стране, исхрана обогаћена фруктозом довела је до хипертриглицеридемије, али без појаве карактеристика масне јетре, док су мишеви на масној исхрани имали изражену стеатозу.
Оба испитивана анимална модела могу бити корисна за ендокриноло- шка истраживања, с тим што анимални модел гојазности индуковане масном храном показује значајнију сличност са карактеристикама гојазности код људи, док је исхрана обогаћена фруктозом бољи анимални
модел за метаболичке промене као што су дислипидемија и адипогенеза., Obesity is one of today’s major health problems and is often associated with chronic
diseases such as dyslipidemia, insulin resistance and atherosclerosis. Although both
genetic and environmental factors can be the cause of obesity, the most common cause
is the modern lifestyle, which includes a hypercaloric diet high in sugar and fat. Controlled
studies in animal models of diet-induced obesity are of great importance for the
investigation of the mechanisms of its development, but also for the improvement of
existing and the development of new therapies.
In our research, we used animal models of obesity in rodents, namely a rat fed a
60% fructose solution in water for 9 weeks and a mouse fed a 60% fat diet for 14 weeks.
Increased energy intake was observed in both animal models, although a high
fructose diet did not lead to an increase in body weight but did lead to an increase in
visceral adipose tissue (VAT) mass. In contrast to fructose, a high-fat diet resulted in
the increase of body and VAT mass. These animals also showed signs of systemic insulin
resistance, which was not the case in the fructose-fed rat model. On the other hand, a
fructose-enriched diet resulted in hypertriglyceridemia, but without the appearance of
fatty liver, while mice on a high-fat diet showed marked steatosis.
Both animal models may be useful for endocrinology research, with the high-fat
diet animal model having greater similarity to features of human obesity, while the
fructose-enriched diet is a better animal model for metabolic changes such as dyslipidemia
and adipogenesis., Gojaznost predstavlja jedan od vodećih zdravstvenih problema savremenog doba, i često je praćena poremećajima kao što su dislipidemija, insulinska rezistencija i ateroskleroza. Iako se u osnovi gojaznosti nalaze i genetički i sredinski faktori, njen najčešći uzročnik je savremeni način života, koji podrazumeva ishranu sa puno šećera i masti. Kontrolisane studije na animalnim modelima gojaznosti izazvane ishranom od velikog su značaja za proučavanje mehanizama njenog nastanka, ali i za unapređenje postojećih i razvoj novih terapija. U našim istraživanjima koristili smo animalne modele gojaznosti na glodarima, i to pacove hranjene sa 60% fruktoze u periodu od devet nedelja i miševe koji su 14 nedelja bili na ishrani sa 60% masti. U oba modela zabeležen je povećan energetski unos, s tim što ishrana obogaćena fruktozom nije dovela do povećanja telesne mase, ali je uticala na povećanje mase visceralnog masnog tkiva. Za razliku od fruktoze, ishrana sa visokim sadržajem masti dovela je do uvećanja ukupne telesne mase i mase visceralnog masnog tkiva miševa. Kod ovih životinja bili su prisutni i znaci sistemske insulinske rezistencije, koja je izostala na modelu pacova hranjenih fruktozom. Sa druge strane, ishrana obogaćena fruktozom dovela je do hipertrigliceridemije, ali bez pojave karakteristika masne jetre, dok su miševi na masnoj ishrani imali izraženu steatozu. Oba ispitivana animalna modela mogu biti korisna za endokrinolo- ška istraživanja, s tim što animalni model gojaznosti indukovane masnom hranom pokazuje značajniju sličnost sa karakteristikama gojaznosti kod ljudi, dok je ishrana obogaćena fruktozom bolji animalni model za metaboličke promene kao što su dislipidemija i adipogeneza.",
publisher = "Beograd: Srpska akademija nauka i umetnosti",
journal = "Novine u gojaznosti 2023: Zbornik radova sa naučnog skupa održanog 9. novembra 2023. godine; 2023 nov 9; Belgrade, Serbia",
title = "Karakteristike animalnih modela gojaznosti indukovanih različitim tipovima ishrane, Карактеристике анималних модела гојазности индукованих различитим типовима исхране, Characteristics of animal models of obesity induced by different food types",
pages = "75-88",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7006"
}

Đorđević, A.. (2024). Karakteristike animalnih modela gojaznosti indukovanih različitim tipovima ishrane. in Novine u gojaznosti 2023: Zbornik radova sa naučnog skupa održanog 9. novembra 2023. godine; 2023 nov 9; Belgrade, Serbia
Beograd: Srpska akademija nauka i umetnosti., 75-88.
https://hdl.handle.net/21.15107/rcub_ibiss_7006

Đorđević A. Karakteristike animalnih modela gojaznosti indukovanih različitim tipovima ishrane. in Novine u gojaznosti 2023: Zbornik radova sa naučnog skupa održanog 9. novembra 2023. godine; 2023 nov 9; Belgrade, Serbia. 2024;:75-88.
https://hdl.handle.net/21.15107/rcub_ibiss_7006 .

Đorđević, Ana, "Karakteristike animalnih modela gojaznosti indukovanih različitim tipovima ishrane" in Novine u gojaznosti 2023: Zbornik radova sa naučnog skupa održanog 9. novembra 2023. godine; 2023 nov 9; Belgrade, Serbia (2024):75-88,
https://hdl.handle.net/21.15107/rcub_ibiss_7006 .

TY  - JOUR
AU  - Challéat, Samuel
AU  - Farrugia, Nicolas
AU  - Froidevaux, Jérémy S P
AU  - Gasc, Amandine
AU  - Pajusco, Nicolas
AU  - Silent Cities project consortium
AU  - Budinski, Ivana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7013
AB  - Political responses to the COVID-19 pandemic led to changes in city soundscapes around the globe. From March to October 2020, a consortium of 261 contributors from 35 countries brought together by the Silent Cities project built a unique soundscape recordings collection to report on local acoustic changes in urban areas. We present this collection here, along with metadata including observational descriptions of the local areas from the contributors, open-source environmental data, open-source confinement levels and calculation of acoustic descriptors. We performed a technical validation of the dataset using statistical models run on a subset of manually annotated soundscapes. Results confirmed the large-scale usability of ecoacoustic indices and automatic sound event recognition in the Silent Cities soundscape collection. We expect this dataset to be useful for research in the multidisciplinary field of environmental sciences.
PB  - Springer Nature
T2  - Scientific Data
T1  - A dataset of acoustic measurements from soundscapes collected worldwide during the COVID-19 pandemic
IS  - 928
VL  - 11
DO  - 10.1038/s41597-024-03611-7
ER  - 

@article{
author = "Challéat, Samuel and Farrugia, Nicolas and Froidevaux, Jérémy S P and Gasc, Amandine and Pajusco, Nicolas and Silent Cities project consortium and Budinski, Ivana",
year = "2024",
abstract = "Political responses to the COVID-19 pandemic led to changes in city soundscapes around the globe. From March to October 2020, a consortium of 261 contributors from 35 countries brought together by the Silent Cities project built a unique soundscape recordings collection to report on local acoustic changes in urban areas. We present this collection here, along with metadata including observational descriptions of the local areas from the contributors, open-source environmental data, open-source confinement levels and calculation of acoustic descriptors. We performed a technical validation of the dataset using statistical models run on a subset of manually annotated soundscapes. Results confirmed the large-scale usability of ecoacoustic indices and automatic sound event recognition in the Silent Cities soundscape collection. We expect this dataset to be useful for research in the multidisciplinary field of environmental sciences.",
publisher = "Springer Nature",
journal = "Scientific Data",
title = "A dataset of acoustic measurements from soundscapes collected worldwide during the COVID-19 pandemic",
number = "928",
volume = "11",
doi = "10.1038/s41597-024-03611-7"
}

Challéat, S., Farrugia, N., Froidevaux, J. S. P., Gasc, A., Pajusco, N., Silent Cities project consortium,& Budinski, I.. (2024). A dataset of acoustic measurements from soundscapes collected worldwide during the COVID-19 pandemic. in Scientific Data
Springer Nature., 11(928).
https://doi.org/10.1038/s41597-024-03611-7

Challéat S, Farrugia N, Froidevaux JSP, Gasc A, Pajusco N, Silent Cities project consortium, Budinski I. A dataset of acoustic measurements from soundscapes collected worldwide during the COVID-19 pandemic. in Scientific Data. 2024;11(928).
doi:10.1038/s41597-024-03611-7 .

Challéat, Samuel, Farrugia, Nicolas, Froidevaux, Jérémy S P, Gasc, Amandine, Pajusco, Nicolas, Silent Cities project consortium, Budinski, Ivana, "A dataset of acoustic measurements from soundscapes collected worldwide during the COVID-19 pandemic" in Scientific Data, 11, no. 928 (2024),
https://doi.org/10.1038/s41597-024-03611-7 . .

TY  - DATA
AU  - Mihailović, Mirjana
AU  - Soković Bajić, Svetlana
AU  - Arambašić Jovanović, Jelena
AU  - Brdarić, Emilija
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Uskoković, Aleksandra
AU  - Rajić, Jovana
AU  - Đorđević, Marija
AU  - Tolinački, Maja
AU  - Golić, Nataša
AU  - Živković, Milica
AU  - Vidaković, Melita
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7005
AB  - Figure S1. Alpha diversity as a parameter of biodiversity within the group,
expressed via Shannon diversity index (A), Simpson diversity index (B),
Chao 1 index (C) and Observed species (D), *represents significance
(p<0.05), ** represents significance (p <0.01).
Figure S2. Beta diversity for comparison of different microbial communities,
PCoA was used for its visuelization
T1  - Supplementary figures for the article:Mihailović, M.; Soković Bajić, S.; Arambašić Jovanović, J.; Brdarić, E.; Dinić, S.; Grdović, N.; Uskoković, A.; Rajić, J.; Đorđević, M.; Tolinački, M.; et al. Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats. Int. J. Mol. Sci.
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_7005
ER  - 

@misc{
author = "Mihailović, Mirjana and Soković Bajić, Svetlana and Arambašić Jovanović, Jelena and Brdarić, Emilija and Dinić, Svetlana and Grdović, Nevena and Uskoković, Aleksandra and Rajić, Jovana and Đorđević, Marija and Tolinački, Maja and Golić, Nataša and Živković, Milica and Vidaković, Melita",
year = "2024",
abstract = "Figure S1. Alpha diversity as a parameter of biodiversity within the group,
expressed via Shannon diversity index (A), Simpson diversity index (B),
Chao 1 index (C) and Observed species (D), *represents significance
(p<0.05), ** represents significance (p <0.01).
Figure S2. Beta diversity for comparison of different microbial communities,
PCoA was used for its visuelization",
title = "Supplementary figures for the article:Mihailović, M.; Soković Bajić, S.; Arambašić Jovanović, J.; Brdarić, E.; Dinić, S.; Grdović, N.; Uskoković, A.; Rajić, J.; Đorđević, M.; Tolinački, M.; et al. Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats. Int. J. Mol. Sci.",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7005"
}

Mihailović, M., Soković Bajić, S., Arambašić Jovanović, J., Brdarić, E., Dinić, S., Grdović, N., Uskoković, A., Rajić, J., Đorđević, M., Tolinački, M., Golić, N., Živković, M.,& Vidaković, M.. (2024). Supplementary figures for the article:Mihailović, M.; Soković Bajić, S.; Arambašić Jovanović, J.; Brdarić, E.; Dinić, S.; Grdović, N.; Uskoković, A.; Rajić, J.; Đorđević, M.; Tolinački, M.; et al. Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats. Int. J. Mol. Sci.. .
https://hdl.handle.net/21.15107/rcub_ibiss_7005

Mihailović M, Soković Bajić S, Arambašić Jovanović J, Brdarić E, Dinić S, Grdović N, Uskoković A, Rajić J, Đorđević M, Tolinački M, Golić N, Živković M, Vidaković M. Supplementary figures for the article:Mihailović, M.; Soković Bajić, S.; Arambašić Jovanović, J.; Brdarić, E.; Dinić, S.; Grdović, N.; Uskoković, A.; Rajić, J.; Đorđević, M.; Tolinački, M.; et al. Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats. Int. J. Mol. Sci.. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_7005 .

Mihailović, Mirjana, Soković Bajić, Svetlana, Arambašić Jovanović, Jelena, Brdarić, Emilija, Dinić, Svetlana, Grdović, Nevena, Uskoković, Aleksandra, Rajić, Jovana, Đorđević, Marija, Tolinački, Maja, Golić, Nataša, Živković, Milica, Vidaković, Melita, "Supplementary figures for the article:Mihailović, M.; Soković Bajić, S.; Arambašić Jovanović, J.; Brdarić, E.; Dinić, S.; Grdović, N.; Uskoković, A.; Rajić, J.; Đorđević, M.; Tolinački, M.; et al. Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats. Int. J. Mol. Sci." (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_7005 .

TY  - JOUR
AU  - Mihailović, Mirjana
AU  - Soković Bajić, Svetlana
AU  - Arambašić Jovanović, Jelena
AU  - Brdarić, Emilija
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Uskoković, Aleksandra
AU  - Rajić, Jovana
AU  - Đorđević, Marija
AU  - Tolinački, Maja
AU  - Golić, Nataša
AU  - Živković, Milica
AU  - Vidaković, Melita
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7004
AB  - Diabetes mellitus, as a chronic metabolic disorder, significantly impacts the pancreas and among other organs, affects duodenal function. Emerging evidence suggests that probiotics can exert beneficial effects on gut health and metabolism. In our previous research, we evaluated the probiotic Lactobacillus paraplantarum BGCG11 primarily for its protective properties against diabetic rats’ damaged liver and kidneys. In this work, we further examined the effects of probiotic strain BGCG11 on the function of the duodenum and pancreas in diabetic rats. We explored the potential mechanisms underlying the probiotic’s effects, focusing on general indicators of diabetes, the architecture and morphology of pancreatic islets, duodenal integrity (measuring the transfer of fluid and serum zonulin level), and the modulation of gut microbiota composition. Our findings reveal the protective and regulatory roles of L. paraplantarum BGCG11 in mitigating diabetes-induced pancreatic and duodenal dysfunction regardless of its application time (pre- or post-treatment), highlighting its therapeutic potential in managing diabetes-related gastrointestinal complications.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats
IS  - 14
VL  - 25
DO  - 10.3390/ijms25147697
SP  - 7697
ER  - 

@article{
author = "Mihailović, Mirjana and Soković Bajić, Svetlana and Arambašić Jovanović, Jelena and Brdarić, Emilija and Dinić, Svetlana and Grdović, Nevena and Uskoković, Aleksandra and Rajić, Jovana and Đorđević, Marija and Tolinački, Maja and Golić, Nataša and Živković, Milica and Vidaković, Melita",
year = "2024",
abstract = "Diabetes mellitus, as a chronic metabolic disorder, significantly impacts the pancreas and among other organs, affects duodenal function. Emerging evidence suggests that probiotics can exert beneficial effects on gut health and metabolism. In our previous research, we evaluated the probiotic Lactobacillus paraplantarum BGCG11 primarily for its protective properties against diabetic rats’ damaged liver and kidneys. In this work, we further examined the effects of probiotic strain BGCG11 on the function of the duodenum and pancreas in diabetic rats. We explored the potential mechanisms underlying the probiotic’s effects, focusing on general indicators of diabetes, the architecture and morphology of pancreatic islets, duodenal integrity (measuring the transfer of fluid and serum zonulin level), and the modulation of gut microbiota composition. Our findings reveal the protective and regulatory roles of L. paraplantarum BGCG11 in mitigating diabetes-induced pancreatic and duodenal dysfunction regardless of its application time (pre- or post-treatment), highlighting its therapeutic potential in managing diabetes-related gastrointestinal complications.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats",
number = "14",
volume = "25",
doi = "10.3390/ijms25147697",
pages = "7697"
}

Mihailović, M., Soković Bajić, S., Arambašić Jovanović, J., Brdarić, E., Dinić, S., Grdović, N., Uskoković, A., Rajić, J., Đorđević, M., Tolinački, M., Golić, N., Živković, M.,& Vidaković, M.. (2024). Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats. in International Journal of Molecular Sciences
Basel: MDPI., 25(14), 7697.
https://doi.org/10.3390/ijms25147697

Mihailović M, Soković Bajić S, Arambašić Jovanović J, Brdarić E, Dinić S, Grdović N, Uskoković A, Rajić J, Đorđević M, Tolinački M, Golić N, Živković M, Vidaković M. Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats. in International Journal of Molecular Sciences. 2024;25(14):7697.
doi:10.3390/ijms25147697 .

Mihailović, Mirjana, Soković Bajić, Svetlana, Arambašić Jovanović, Jelena, Brdarić, Emilija, Dinić, Svetlana, Grdović, Nevena, Uskoković, Aleksandra, Rajić, Jovana, Đorđević, Marija, Tolinački, Maja, Golić, Nataša, Živković, Milica, Vidaković, Melita, "Beneficial Effects of Probiotic Lactobacillus paraplantarum BGCG11 on Pancreatic and Duodenum Function in Diabetic Rats" in International Journal of Molecular Sciences, 25, no. 14 (2024):7697,
https://doi.org/10.3390/ijms25147697 . .

TY  - JOUR
AU  - Oreščanin Dušić, Zorana
AU  - Kovačević, Sanja
AU  - Ristić, Nataša
AU  - Vojnović Milutinović, Danijela
AU  - Vidonja Uzelac, Teodora
AU  - Blagojević, Duško
AU  - Đorđević, Ana
AU  - Brkljačić, Jelena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7008
AB  - Increased fructose consumption and chronic stress, the major characteristics of modern
lifestyle, impact human health; however, the consequences of their combination on the uterus remain
understudied. In this study, we investigated contractile activity, morphology, and intracellular
activity of antioxidant enzymes in uteri from virginWistar rats subjected to liquid fructose supplementation
and/or unpredictable stress over 9 weeks. Contractile activity and uterine response to
oxytocin or adrenaline were examined ex vivo using isolated bath chambers. Fructose supplementation,
irrespective of stress, affected uterine morphology by increasing endometrium while decreasing
myometrium volume density, attenuated uterine response to increasing doses of oxytocin, and increased
glutathione peroxidase activity. Stress, irrespective of fructose, attenuated dose-dependent
adrenaline-induced uterine relaxation. Stress, when applied solely, decreased mitochondrial superoxide
dismutase activity. In the combined treatment, irregular estrous cycles and both reduced
response to oxytocin and to adrenaline (as a consequence of fructose consumption and exposure to
stress), along with fructose-related alteration of uterine morphology, were detected. In conclusion,
fructose and stress affect uterine contractile activity, irrespective of each other, by inducing completely
distinct responses in isolated uteri. In the combined treatment, the effects of both factors were
evident, suggesting that the combination exerts more detrimental effects on the uterus than each
factor individually.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Effects of Liquid Fructose Supplementation and Chronic Unpredictable Stress on Uterine Contractile Activity in Nonpregnant Rats
IS  - 12
VL  - 25
DO  - 10.3390/ijms25126770
SP  - 6770
ER  - 

@article{
author = "Oreščanin Dušić, Zorana and Kovačević, Sanja and Ristić, Nataša and Vojnović Milutinović, Danijela and Vidonja Uzelac, Teodora and Blagojević, Duško and Đorđević, Ana and Brkljačić, Jelena",
year = "2024",
abstract = "Increased fructose consumption and chronic stress, the major characteristics of modern
lifestyle, impact human health; however, the consequences of their combination on the uterus remain
understudied. In this study, we investigated contractile activity, morphology, and intracellular
activity of antioxidant enzymes in uteri from virginWistar rats subjected to liquid fructose supplementation
and/or unpredictable stress over 9 weeks. Contractile activity and uterine response to
oxytocin or adrenaline were examined ex vivo using isolated bath chambers. Fructose supplementation,
irrespective of stress, affected uterine morphology by increasing endometrium while decreasing
myometrium volume density, attenuated uterine response to increasing doses of oxytocin, and increased
glutathione peroxidase activity. Stress, irrespective of fructose, attenuated dose-dependent
adrenaline-induced uterine relaxation. Stress, when applied solely, decreased mitochondrial superoxide
dismutase activity. In the combined treatment, irregular estrous cycles and both reduced
response to oxytocin and to adrenaline (as a consequence of fructose consumption and exposure to
stress), along with fructose-related alteration of uterine morphology, were detected. In conclusion,
fructose and stress affect uterine contractile activity, irrespective of each other, by inducing completely
distinct responses in isolated uteri. In the combined treatment, the effects of both factors were
evident, suggesting that the combination exerts more detrimental effects on the uterus than each
factor individually.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effects of Liquid Fructose Supplementation and Chronic Unpredictable Stress on Uterine Contractile Activity in Nonpregnant Rats",
number = "12",
volume = "25",
doi = "10.3390/ijms25126770",
pages = "6770"
}

Oreščanin Dušić, Z., Kovačević, S., Ristić, N., Vojnović Milutinović, D., Vidonja Uzelac, T., Blagojević, D., Đorđević, A.,& Brkljačić, J.. (2024). Effects of Liquid Fructose Supplementation and Chronic Unpredictable Stress on Uterine Contractile Activity in Nonpregnant Rats. in International Journal of Molecular Sciences
Basel: MDPI., 25(12), 6770.
https://doi.org/10.3390/ijms25126770

Oreščanin Dušić Z, Kovačević S, Ristić N, Vojnović Milutinović D, Vidonja Uzelac T, Blagojević D, Đorđević A, Brkljačić J. Effects of Liquid Fructose Supplementation and Chronic Unpredictable Stress on Uterine Contractile Activity in Nonpregnant Rats. in International Journal of Molecular Sciences. 2024;25(12):6770.
doi:10.3390/ijms25126770 .

Oreščanin Dušić, Zorana, Kovačević, Sanja, Ristić, Nataša, Vojnović Milutinović, Danijela, Vidonja Uzelac, Teodora, Blagojević, Duško, Đorđević, Ana, Brkljačić, Jelena, "Effects of Liquid Fructose Supplementation and Chronic Unpredictable Stress on Uterine Contractile Activity in Nonpregnant Rats" in International Journal of Molecular Sciences, 25, no. 12 (2024):6770,
https://doi.org/10.3390/ijms25126770 . .

TY  - CONF
AU  - Jovanović Stojanov, Sofija
AU  - Podolski-Renić, Ana
AU  - Dinić, Jelena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
AU  - Pešić, Milica
AU  - Dragoj, Miodrag
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7012
AB  - In our study, we aimed to develop a long-term three-dimensional (3D) model for glioblastoma cell culture to facilitate more reliable drug response studies. We cultured human U87 glioblastoma cells in alginate microfibers for a period of 28 days. Throughout this time, we monitored cell growth, viability, morphology, and aggregation in the 3D culture using fluorescent and confocal microscopy. Calcein-AM/propidium iodide staining was performed every seven days.

We validated the glioblastoma 3D model by subjecting the cells to temozolomide (TMZ) treatments for three consecutive days, starting from the 7th day of culturing cells in alginate microfibers. After a recovery period of 18 days, we evaluated cell viability using MTT assays and assessed the expression of resistance-related genes (MGMT and ABCB1) using qPCR. We also applied the same TMZ treatment schedule to cells cultured in a two-dimensional (2D) setting for comparison purposes.

Our results showed that within the long-term 3D model system in alginate fibers, the U87 cells remained viable for the entire 28-day period. Furthermore, on day 7, we observed that the cells formed visible aggregates oriented towards the periphery of the microfibers. Upon TMZ treatment, we observed a reduction in cell growth, as well as an increase in the expression of drug resistance-related genes. This effect was more pronounced in the 3D culture compared to the 2D culture.

In conclusion, we established a novel glioblastoma 3D model system, which could be particularly valuable for conducting long-term drug testing and optimizing treatment strategies.
PB  - Ljubljana: National Institute of Biology
C3  - Book of Abstracts: 1st Net4Brain Annual Meeting: Closing the translational gap in brain cancer treatment; 2024 Sep 4-6; Ljubljana, Slovenia
T1  - 3D Glioblastoma Cell Culture within Alginate Microfibers for Long-Term Evaluation of Drug Effects
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_7012
ER  - 

@conference{
author = "Jovanović Stojanov, Sofija and Podolski-Renić, Ana and Dinić, Jelena and Stojkovska, Jasmina and Obradović, Bojana and Pešić, Milica and Dragoj, Miodrag",
year = "2024",
abstract = "In our study, we aimed to develop a long-term three-dimensional (3D) model for glioblastoma cell culture to facilitate more reliable drug response studies. We cultured human U87 glioblastoma cells in alginate microfibers for a period of 28 days. Throughout this time, we monitored cell growth, viability, morphology, and aggregation in the 3D culture using fluorescent and confocal microscopy. Calcein-AM/propidium iodide staining was performed every seven days.

We validated the glioblastoma 3D model by subjecting the cells to temozolomide (TMZ) treatments for three consecutive days, starting from the 7th day of culturing cells in alginate microfibers. After a recovery period of 18 days, we evaluated cell viability using MTT assays and assessed the expression of resistance-related genes (MGMT and ABCB1) using qPCR. We also applied the same TMZ treatment schedule to cells cultured in a two-dimensional (2D) setting for comparison purposes.

Our results showed that within the long-term 3D model system in alginate fibers, the U87 cells remained viable for the entire 28-day period. Furthermore, on day 7, we observed that the cells formed visible aggregates oriented towards the periphery of the microfibers. Upon TMZ treatment, we observed a reduction in cell growth, as well as an increase in the expression of drug resistance-related genes. This effect was more pronounced in the 3D culture compared to the 2D culture.

In conclusion, we established a novel glioblastoma 3D model system, which could be particularly valuable for conducting long-term drug testing and optimizing treatment strategies.",
publisher = "Ljubljana: National Institute of Biology",
journal = "Book of Abstracts: 1st Net4Brain Annual Meeting: Closing the translational gap in brain cancer treatment; 2024 Sep 4-6; Ljubljana, Slovenia",
title = "3D Glioblastoma Cell Culture within Alginate Microfibers for Long-Term Evaluation of Drug Effects",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7012"
}

Jovanović Stojanov, S., Podolski-Renić, A., Dinić, J., Stojkovska, J., Obradović, B., Pešić, M.,& Dragoj, M.. (2024). 3D Glioblastoma Cell Culture within Alginate Microfibers for Long-Term Evaluation of Drug Effects. in Book of Abstracts: 1st Net4Brain Annual Meeting: Closing the translational gap in brain cancer treatment; 2024 Sep 4-6; Ljubljana, Slovenia
Ljubljana: National Institute of Biology., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_7012

Jovanović Stojanov S, Podolski-Renić A, Dinić J, Stojkovska J, Obradović B, Pešić M, Dragoj M. 3D Glioblastoma Cell Culture within Alginate Microfibers for Long-Term Evaluation of Drug Effects. in Book of Abstracts: 1st Net4Brain Annual Meeting: Closing the translational gap in brain cancer treatment; 2024 Sep 4-6; Ljubljana, Slovenia. 2024;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_7012 .

Jovanović Stojanov, Sofija, Podolski-Renić, Ana, Dinić, Jelena, Stojkovska, Jasmina, Obradović, Bojana, Pešić, Milica, Dragoj, Miodrag, "3D Glioblastoma Cell Culture within Alginate Microfibers for Long-Term Evaluation of Drug Effects" in Book of Abstracts: 1st Net4Brain Annual Meeting: Closing the translational gap in brain cancer treatment; 2024 Sep 4-6; Ljubljana, Slovenia (2024):28,
https://hdl.handle.net/21.15107/rcub_ibiss_7012 .

TY  - JOUR
AU  - Veličković, Nataša
AU  - Mićić, Bojana
AU  - Teofilović, Ana
AU  - Milovanović, Milena
AU  - Jovanović, Mirna
AU  - Đorđević, Ana
AU  - Macut, Djuro
AU  - Vojnović Milutinović, Danijela
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7009
AB  - Background: Polycystic ovary syndrome (PCOS) is the most common endocrine
disorder in women of reproductive age and is closely associated with chronic
low-grade inflammation and insulin resistance. To clarify the contribution of
prepubertal weight gain to the development of insulin resistance in PCOS, we
investigated the effects of early postnatal overfeeding on inflammatory and
energy-sensing pathways as well as on markers of insulin signaling in the liver
of the PCOS rat model.
Methods: Obesity induced by overfeeding was achieved by reducing litter size,
while the PCOS-like condition was developed by treatment with 5a-
dihydrotestosterone (DHT). Western blot and qPCR were used to analyze the
expression of pro-inflammatory transcription factors and cytokines, as well as
markers of the energy sensing and insulin signaling pathways.
Results: The results showed that hepatic insulin sensitivity was impaired only in
DHT-treated rats raised in small litters, as evidenced by increased
phosphorylation of IRS1 on Ser307 and decreased expression of total IRS1.
Postnatal overfeeding stimulated JNK1 activation independent of
hyperandrogenemia; nevertheless, the synergistic effect of both factors
triggered NLRP3 activation and increased IL1b expression in the small litter
DHT-treated group. This pro-inflammatory state was accompanied by
decreased activatory phosphorylation of AMPK and reduced levels of its
protein targets.
Conclusions: Overfeeding in the early postnatal period leads to a decrease in
hepatic insulin sensitivity in the rat model of PCOS, which is associated with
decreased activation of AMPK and stimulation of the hepatic NLRP3-IL1b
signaling pathway. Accordingly, the inhibition of NLRP3 activation could
provide a basis for the development of new therapeutic strategies for the
treatment of insulin resistance in women with PCOS.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Endocrinology
T1  - Overfeeding in the early postnatal period aggravates inflammation and hepatic insulin sensitivity in the 5a-dihydrotestosterone-induced animal model of PCOS
VL  - 15
DO  - 10.3389/fendo.2024.1402905
SP  - 1402905
ER  - 

@article{
author = "Veličković, Nataša and Mićić, Bojana and Teofilović, Ana and Milovanović, Milena and Jovanović, Mirna and Đorđević, Ana and Macut, Djuro and Vojnović Milutinović, Danijela",
year = "2024",
abstract = "Background: Polycystic ovary syndrome (PCOS) is the most common endocrine
disorder in women of reproductive age and is closely associated with chronic
low-grade inflammation and insulin resistance. To clarify the contribution of
prepubertal weight gain to the development of insulin resistance in PCOS, we
investigated the effects of early postnatal overfeeding on inflammatory and
energy-sensing pathways as well as on markers of insulin signaling in the liver
of the PCOS rat model.
Methods: Obesity induced by overfeeding was achieved by reducing litter size,
while the PCOS-like condition was developed by treatment with 5a-
dihydrotestosterone (DHT). Western blot and qPCR were used to analyze the
expression of pro-inflammatory transcription factors and cytokines, as well as
markers of the energy sensing and insulin signaling pathways.
Results: The results showed that hepatic insulin sensitivity was impaired only in
DHT-treated rats raised in small litters, as evidenced by increased
phosphorylation of IRS1 on Ser307 and decreased expression of total IRS1.
Postnatal overfeeding stimulated JNK1 activation independent of
hyperandrogenemia; nevertheless, the synergistic effect of both factors
triggered NLRP3 activation and increased IL1b expression in the small litter
DHT-treated group. This pro-inflammatory state was accompanied by
decreased activatory phosphorylation of AMPK and reduced levels of its
protein targets.
Conclusions: Overfeeding in the early postnatal period leads to a decrease in
hepatic insulin sensitivity in the rat model of PCOS, which is associated with
decreased activation of AMPK and stimulation of the hepatic NLRP3-IL1b
signaling pathway. Accordingly, the inhibition of NLRP3 activation could
provide a basis for the development of new therapeutic strategies for the
treatment of insulin resistance in women with PCOS.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Endocrinology",
title = "Overfeeding in the early postnatal period aggravates inflammation and hepatic insulin sensitivity in the 5a-dihydrotestosterone-induced animal model of PCOS",
volume = "15",
doi = "10.3389/fendo.2024.1402905",
pages = "1402905"
}

Veličković, N., Mićić, B., Teofilović, A., Milovanović, M., Jovanović, M., Đorđević, A., Macut, D.,& Vojnović Milutinović, D.. (2024). Overfeeding in the early postnatal period aggravates inflammation and hepatic insulin sensitivity in the 5a-dihydrotestosterone-induced animal model of PCOS. in Frontiers in Endocrinology
Lausanne: Frontiers Media SA., 15, 1402905.
https://doi.org/10.3389/fendo.2024.1402905

Veličković N, Mićić B, Teofilović A, Milovanović M, Jovanović M, Đorđević A, Macut D, Vojnović Milutinović D. Overfeeding in the early postnatal period aggravates inflammation and hepatic insulin sensitivity in the 5a-dihydrotestosterone-induced animal model of PCOS. in Frontiers in Endocrinology. 2024;15:1402905.
doi:10.3389/fendo.2024.1402905 .

Veličković, Nataša, Mićić, Bojana, Teofilović, Ana, Milovanović, Milena, Jovanović, Mirna, Đorđević, Ana, Macut, Djuro, Vojnović Milutinović, Danijela, "Overfeeding in the early postnatal period aggravates inflammation and hepatic insulin sensitivity in the 5a-dihydrotestosterone-induced animal model of PCOS" in Frontiers in Endocrinology, 15 (2024):1402905,
https://doi.org/10.3389/fendo.2024.1402905 . .

TY  - CONF
AU  - Vojnović Milutinović, Danijela
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7007
AB  - Синдром полицистичних јајника је један од најчешћих ендокриних и метаболичких поремећаја код жена у репродуктивном периоду живота. Први знаци синдрома се јављају код девојчица у периоду пре пубертета и удружени су са гојазношћу, која у каснијем адоле- сцентном периоду са хиперандрогенемијом и/или хиперинсулинемијом води ка адултном синдрому полицистичних јајника. У досадашњим истраживањима синдрома полицистичних јајника коришћени су различити анимални модели, од којих се највећи број заснивао на употреби андрогена у пренаталном или постнаталном периоду. Сви ови модели пружају важан приступ одговорима на питања о молекуларним основама патофизиолошких промена, пореклу синдрома, наслеђивању, као и начинима његове превенције и терапије. Овај кратак приказни чланак резимира недавна истраживања на анималном моделу која дају допринос одговору на питање у којој мери препубертална гојазност и/или хиперандрогенемија доприносе развоју метаболичких поремећаја који су карактеристични за синдром полицистичних јајника.
AB  - Polycystic ovary syndrome is one of the most common endocrine and metabolic
disorders in women in the reproductive phase of life. The first signs of the syndrome
appear in girls before puberty and are associated with obesity, which in later adolescence
together with hyperandrogenemia and/or hyperinsulinemia leads to adult polycystic
ovary syndrome. Previous studies on polycystic ovary syndrome have used various
animal models, most of which were based on the use of androgens in the prenatal
or postnatal period. All of these models provide an important approach to answering
questions about the molecular basis of the pathophysiological changes, the origin of
the syndrome, heredity, and possibilities for prevention and therapy. This short review
article summarizes recent research on animal model that contributes to answering the
question to what extent prepubertal obesity and/or hyperandrogenemia contribute to
the development of the metabolic disorders characteristic of polycystic ovary syndrome.
AB  - Sindrom policističnih jajnika je jedan od najčešćih endokrinih i metaboličkih poremećaja kod žena u reproduktivnom periodu života. Prvi znaci sindroma se javljaju kod devojčica u periodu pre puberteta i udruženi su sa gojaznošću, koja u kasnijem adole- scentnom periodu sa hiperandrogenemijom i/ili hiperinsulinemijom vodi ka adultnom sindromu policističnih jajnika. U dosadašnjim istraživanjima sindroma policističnih jajnika korišćeni su različiti animalni modeli, od kojih se najveći broj zasnivao na upotrebi androgena u prenatalnom ili postnatalnom periodu. Svi ovi modeli pružaju važan pristup odgovorima na pitanja o molekularnim osnovama patofizioloških promena, poreklu sindroma, nasleđivanju, kao i načinima njegove prevencije i terapije. Ovaj kratak prikazni članak rezimira nedavna istraživanja na animalnom modelu koja daju doprinos odgovoru na pitanje u kojoj meri prepubertalna gojaznost i/ili hiperandrogenemija doprinose razvoju metaboličkih poremećaja koji su karakteristični za sindrom policističnih jajnika.
PB  - Srpska akademija nauka i umetnosti
PB  - Beograd: Srpska akademija nauka i umetnosti
C3  - Novine u gojaznosti 2023: Zbornik radova sa naučnog skupa održanog 9. novembra 2023. godine; 2023 nov 9; Belgrade, Serbia
T1  - Odnos gojaznosti i insulinske senzitivnosti u animalnom modelu sindroma policističnih jajnika
T1  - The relationship between obesity and insulin sensitivity in an animal model of polycistic ovary syndrome
T1  - Однос гојазности и инсулинске сензитивности у анималном моделу синдрома полицистичних јајника
SP  - 89
EP  - 99
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_7007
ER  - 

@conference{
author = "Vojnović Milutinović, Danijela",
year = "2024",
abstract = "Синдром полицистичних јајника је један од најчешћих ендокриних и метаболичких поремећаја код жена у репродуктивном периоду живота. Први знаци синдрома се јављају код девојчица у периоду пре пубертета и удружени су са гојазношћу, која у каснијем адоле- сцентном периоду са хиперандрогенемијом и/или хиперинсулинемијом води ка адултном синдрому полицистичних јајника. У досадашњим истраживањима синдрома полицистичних јајника коришћени су различити анимални модели, од којих се највећи број заснивао на употреби андрогена у пренаталном или постнаталном периоду. Сви ови модели пружају важан приступ одговорима на питања о молекуларним основама патофизиолошких промена, пореклу синдрома, наслеђивању, као и начинима његове превенције и терапије. Овај кратак приказни чланак резимира недавна истраживања на анималном моделу која дају допринос одговору на питање у којој мери препубертална гојазност и/или хиперандрогенемија доприносе развоју метаболичких поремећаја који су карактеристични за синдром полицистичних јајника., Polycystic ovary syndrome is one of the most common endocrine and metabolic
disorders in women in the reproductive phase of life. The first signs of the syndrome
appear in girls before puberty and are associated with obesity, which in later adolescence
together with hyperandrogenemia and/or hyperinsulinemia leads to adult polycystic
ovary syndrome. Previous studies on polycystic ovary syndrome have used various
animal models, most of which were based on the use of androgens in the prenatal
or postnatal period. All of these models provide an important approach to answering
questions about the molecular basis of the pathophysiological changes, the origin of
the syndrome, heredity, and possibilities for prevention and therapy. This short review
article summarizes recent research on animal model that contributes to answering the
question to what extent prepubertal obesity and/or hyperandrogenemia contribute to
the development of the metabolic disorders characteristic of polycystic ovary syndrome., Sindrom policističnih jajnika je jedan od najčešćih endokrinih i metaboličkih poremećaja kod žena u reproduktivnom periodu života. Prvi znaci sindroma se javljaju kod devojčica u periodu pre puberteta i udruženi su sa gojaznošću, koja u kasnijem adole- scentnom periodu sa hiperandrogenemijom i/ili hiperinsulinemijom vodi ka adultnom sindromu policističnih jajnika. U dosadašnjim istraživanjima sindroma policističnih jajnika korišćeni su različiti animalni modeli, od kojih se najveći broj zasnivao na upotrebi androgena u prenatalnom ili postnatalnom periodu. Svi ovi modeli pružaju važan pristup odgovorima na pitanja o molekularnim osnovama patofizioloških promena, poreklu sindroma, nasleđivanju, kao i načinima njegove prevencije i terapije. Ovaj kratak prikazni članak rezimira nedavna istraživanja na animalnom modelu koja daju doprinos odgovoru na pitanje u kojoj meri prepubertalna gojaznost i/ili hiperandrogenemija doprinose razvoju metaboličkih poremećaja koji su karakteristični za sindrom policističnih jajnika.",
publisher = "Srpska akademija nauka i umetnosti, Beograd: Srpska akademija nauka i umetnosti",
journal = "Novine u gojaznosti 2023: Zbornik radova sa naučnog skupa održanog 9. novembra 2023. godine; 2023 nov 9; Belgrade, Serbia",
title = "Odnos gojaznosti i insulinske senzitivnosti u animalnom modelu sindroma policističnih jajnika, The relationship between obesity and insulin sensitivity in an animal model of polycistic ovary syndrome, Однос гојазности и инсулинске сензитивности у анималном моделу синдрома полицистичних јајника",
pages = "89-99",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7007"
}

Vojnović Milutinović, D.. (2024). Odnos gojaznosti i insulinske senzitivnosti u animalnom modelu sindroma policističnih jajnika. in Novine u gojaznosti 2023: Zbornik radova sa naučnog skupa održanog 9. novembra 2023. godine; 2023 nov 9; Belgrade, Serbia
Srpska akademija nauka i umetnosti., 89-99.
https://hdl.handle.net/21.15107/rcub_ibiss_7007

Vojnović Milutinović D. Odnos gojaznosti i insulinske senzitivnosti u animalnom modelu sindroma policističnih jajnika. in Novine u gojaznosti 2023: Zbornik radova sa naučnog skupa održanog 9. novembra 2023. godine; 2023 nov 9; Belgrade, Serbia. 2024;:89-99.
https://hdl.handle.net/21.15107/rcub_ibiss_7007 .

Vojnović Milutinović, Danijela, "Odnos gojaznosti i insulinske senzitivnosti u animalnom modelu sindroma policističnih jajnika" in Novine u gojaznosti 2023: Zbornik radova sa naučnog skupa održanog 9. novembra 2023. godine; 2023 nov 9; Belgrade, Serbia (2024):89-99,
https://hdl.handle.net/21.15107/rcub_ibiss_7007 .