TY  - JOUR
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6553
AB  - Introduction: In the present study, we assessed the effects of the hyper- harmonized- 
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
 Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects 
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis 
through machine learning (ML) using artificial neural networks (ANNs) to classify the 
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment 
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in 
cortical and hippocampal tissue were assessed.
 Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in 
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not 
induce the activation of glia (astrocytes and microglia) nor did it negatively affect 
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
 Conclusion: The obtained results point to the potential of 3HFWC, when applied in 
the presymptomatic phase of AD, to interfere with amyloid plaque formation without 
inducing AD- related pathological processes such as neuroinflammation, gliosis, and 
synaptic vulnerability.
PB  - Wiley
T2  - CNS Neuroscience and Therapeutics
T1  - The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease
IS  - 3
VL  - 30
DO  - 10.1111/cns.14188
SP  - e14188
ER  - 

@article{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2024",
abstract = "Introduction: In the present study, we assessed the effects of the hyper- harmonized- 
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
 Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects 
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis 
through machine learning (ML) using artificial neural networks (ANNs) to classify the 
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment 
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in 
cortical and hippocampal tissue were assessed.
 Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in 
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not 
induce the activation of glia (astrocytes and microglia) nor did it negatively affect 
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
 Conclusion: The obtained results point to the potential of 3HFWC, when applied in 
the presymptomatic phase of AD, to interfere with amyloid plaque formation without 
inducing AD- related pathological processes such as neuroinflammation, gliosis, and 
synaptic vulnerability.",
publisher = "Wiley",
journal = "CNS Neuroscience and Therapeutics",
title = "The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease",
number = "3",
volume = "30",
doi = "10.1111/cns.14188",
pages = "e14188"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2024). The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease. in CNS Neuroscience and Therapeutics
Wiley., 30(3), e14188.
https://doi.org/10.1111/cns.14188

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease. in CNS Neuroscience and Therapeutics. 2024;30(3):e14188.
doi:10.1111/cns.14188 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease" in CNS Neuroscience and Therapeutics, 30, no. 3 (2024):e14188,
https://doi.org/10.1111/cns.14188 . .

TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Živanović, Ana
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Major, Tamara
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6548
AB  - Cerebral amyloid angiopathy (CAA) is characterized by amyloid (A ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of A is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The A accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 Mand12M5xFADand12MWTretinas. Theincrease in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 MWTretinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiolog ical (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease
IS  - 18
VL  - 24
DO  - 10.3390/ijms241814092
SP  - 14092
ER  - 

@article{
author = "Jovanović Macura, Irena and Živanović, Ana and Perović, Milka and Ćirić, Jelena and Major, Tamara and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Cerebral amyloid angiopathy (CAA) is characterized by amyloid (A ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of A is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The A accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 Mand12M5xFADand12MWTretinas. Theincrease in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 MWTretinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiolog ical (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease",
number = "18",
volume = "24",
doi = "10.3390/ijms241814092",
pages = "14092"
}

Jovanović Macura, I., Živanović, A., Perović, M., Ćirić, J., Major, T., Kanazir, S.,& Ivković, S.. (2023). The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease. in International Journal of Molecular Sciences
Basel: MDPI., 24(18), 14092.
https://doi.org/10.3390/ijms241814092

Jovanović Macura I, Živanović A, Perović M, Ćirić J, Major T, Kanazir S, Ivković S. The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease. in International Journal of Molecular Sciences. 2023;24(18):14092.
doi:10.3390/ijms241814092 .

Jovanović Macura, Irena, Živanović, Ana, Perović, Milka, Ćirić, Jelena, Major, Tamara, Kanazir, Selma, Ivković, Sanja, "The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease" in International Journal of Molecular Sciences, 24, no. 18 (2023):14092,
https://doi.org/10.3390/ijms241814092 . .

TY  - JOUR
AU  - Todorović, Smilja
AU  - Simeunović, Valentina
AU  - Prvulović, Milica
AU  - Dakić, Tamara
AU  - Jevđović, Tanja
AU  - Sokanović, Srđan
AU  - Kanazir, Selma
AU  - Mladenović, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6373
AB  - Insulin is known to be a key hormone in the regulation of peripheral glucose homeostasis, but beyond that, its effects on the brain are now undisputed. Impairments in insulin signaling in the brain, including changes in insulin levels, are thought to contribute significantly to declines in cognitive performance, especially during aging. As one of the most widely studied experimental interventions, dietary restriction (DR) is considered to delay the neurodegenerative processes associated with aging. Recently, however, data began to suggest that the onset and duration of a restrictive diet play a critical role in the putative beneficial outcome. Because the effects of DR on insulin signaling in the brain have been poorly studied, we decided to examine the effects of DR that differed in onset and duration: long-term DR (LTDR), medium-term DR (MTDR), and short-term DR (STDR) on the expression of proteins involved in insulin signaling in the hippocampus of 18- and 24-month-old male Wistar rats. We found that DR-induced changes in insulin levels in the brain may be independent of what happens in the periphery after restricted feeding. Significantly changed insulin content in the hippocampus, together with altered insulin signaling were found under the influence of DR, but the outcome was highly dependent on the onset and duration of DR.
PB  - Hoboken: John Wiley and Sons
T2  - Biofactors
T1  - Dietary restriction alters insulin signaling pathway in the brain
DO  - 10.1002/biof.2018
ER  - 

@article{
author = "Todorović, Smilja and Simeunović, Valentina and Prvulović, Milica and Dakić, Tamara and Jevđović, Tanja and Sokanović, Srđan and Kanazir, Selma and Mladenović, Aleksandra",
year = "2023",
abstract = "Insulin is known to be a key hormone in the regulation of peripheral glucose homeostasis, but beyond that, its effects on the brain are now undisputed. Impairments in insulin signaling in the brain, including changes in insulin levels, are thought to contribute significantly to declines in cognitive performance, especially during aging. As one of the most widely studied experimental interventions, dietary restriction (DR) is considered to delay the neurodegenerative processes associated with aging. Recently, however, data began to suggest that the onset and duration of a restrictive diet play a critical role in the putative beneficial outcome. Because the effects of DR on insulin signaling in the brain have been poorly studied, we decided to examine the effects of DR that differed in onset and duration: long-term DR (LTDR), medium-term DR (MTDR), and short-term DR (STDR) on the expression of proteins involved in insulin signaling in the hippocampus of 18- and 24-month-old male Wistar rats. We found that DR-induced changes in insulin levels in the brain may be independent of what happens in the periphery after restricted feeding. Significantly changed insulin content in the hippocampus, together with altered insulin signaling were found under the influence of DR, but the outcome was highly dependent on the onset and duration of DR.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Biofactors",
title = "Dietary restriction alters insulin signaling pathway in the brain",
doi = "10.1002/biof.2018"
}

Todorović, S., Simeunović, V., Prvulović, M., Dakić, T., Jevđović, T., Sokanović, S., Kanazir, S.,& Mladenović, A.. (2023). Dietary restriction alters insulin signaling pathway in the brain. in Biofactors
Hoboken: John Wiley and Sons..
https://doi.org/10.1002/biof.2018

Todorović S, Simeunović V, Prvulović M, Dakić T, Jevđović T, Sokanović S, Kanazir S, Mladenović A. Dietary restriction alters insulin signaling pathway in the brain. in Biofactors. 2023;.
doi:10.1002/biof.2018 .

Todorović, Smilja, Simeunović, Valentina, Prvulović, Milica, Dakić, Tamara, Jevđović, Tanja, Sokanović, Srđan, Kanazir, Selma, Mladenović, Aleksandra, "Dietary restriction alters insulin signaling pathway in the brain" in Biofactors (2023),
https://doi.org/10.1002/biof.2018 . .

TY  - CONF
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Sobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5858
AB  - Mfsd2a is expressed mainly in the endothelial cells and is an essential regulator of 
blood vessel transcytosis. Therefore, decrease in Mfsd2a expression can be a risk 
factor for developing leaky blood vessels. Mfsd2a is also the main docosahexaenoic 
acid (DHA, C22:6n3) transporter. DHA, an omega-3 fatty acid, is one of the main 
structural lipids of the neuronal and vascular retina, crucial for the normal functioning 
of photoreceptors (PRs). However, the capacity of the retina to synthesize DHA is 
limited, and the maintenance of retinal DHA content relies on the uptake from blood borne lipids. The currently recommended FO doses yielded low PUFAs tissue 
bioavailability, and supplementation with higher doses has been increasingly 
recommended. Nevertheless, the effects of higher FO doses on retinal Mfsd2a 
expression and blood vessels coverage are unknown.
Western blot and qPCR analyses showed that high dose FO supplementation increased 
Mfsd2a expression in the retina. Immunohistochemical analyses of Mfsd2a expression 
on retinal blood vessels (labeled with 488-conjugated Lycopersicon esculentum, 
lectin) and subsequent ImageJ analyses revealed 1.32-fold increase in the Mfsd2a 
retinal blood vessel coverage. In the same time the pericyte blood vessel coverage 
(CD13+ cells) was not affected with FO supplementation, and the increase in Mfsd2a 
blood vessel expression is not the result of the increased pericyte coverage. 
Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of 
the retinal blood vessels that can serve either as prophylaxis in the healthy eye or as an 
adjuvant in developing targeted manipulations of the barrier during diseases.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5858
ER  - 

@conference{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Sobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Mfsd2a is expressed mainly in the endothelial cells and is an essential regulator of 
blood vessel transcytosis. Therefore, decrease in Mfsd2a expression can be a risk 
factor for developing leaky blood vessels. Mfsd2a is also the main docosahexaenoic 
acid (DHA, C22:6n3) transporter. DHA, an omega-3 fatty acid, is one of the main 
structural lipids of the neuronal and vascular retina, crucial for the normal functioning 
of photoreceptors (PRs). However, the capacity of the retina to synthesize DHA is 
limited, and the maintenance of retinal DHA content relies on the uptake from blood borne lipids. The currently recommended FO doses yielded low PUFAs tissue 
bioavailability, and supplementation with higher doses has been increasingly 
recommended. Nevertheless, the effects of higher FO doses on retinal Mfsd2a 
expression and blood vessels coverage are unknown.
Western blot and qPCR analyses showed that high dose FO supplementation increased 
Mfsd2a expression in the retina. Immunohistochemical analyses of Mfsd2a expression 
on retinal blood vessels (labeled with 488-conjugated Lycopersicon esculentum, 
lectin) and subsequent ImageJ analyses revealed 1.32-fold increase in the Mfsd2a 
retinal blood vessel coverage. In the same time the pericyte blood vessel coverage 
(CD13+ cells) was not affected with FO supplementation, and the increase in Mfsd2a 
blood vessel expression is not the result of the increased pericyte coverage. 
Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of 
the retinal blood vessels that can serve either as prophylaxis in the healthy eye or as an 
adjuvant in developing targeted manipulations of the barrier during diseases.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice",
pages = "66",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5858"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Sobajić, S., Kanazir, S.,& Ivković, S.. (2023). The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 66.
https://hdl.handle.net/21.15107/rcub_ibiss_5858

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Sobajić S, Kanazir S, Ivković S. The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:66.
https://hdl.handle.net/21.15107/rcub_ibiss_5858 .

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Sobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):66,
https://hdl.handle.net/21.15107/rcub_ibiss_5858 .

TY  - CONF
AU  - Mladenović, Aleksandra
AU  - Prvulović, Milica
AU  - Milanović, Desanka
AU  - Sokanović, Srđan
AU  - Vukojević, Anđela
AU  - Simeunović, Valentina
AU  - Kanazir, Selma
AU  - Todorović, Smilja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5852
AB  - Dietary restriction (DR) is considered the gold standard in anti-aging treatment. Since 
the first study on the beneficial effects of DR in 1935, countless research articles have 
demonstrated numerous positive effects of DR. DR is often referred to as the elixir of 
youth due to its proven ability to extend lifespan in various species. The beneficial 
effects of DR are observed in many organ systems, including the prevention of age related diabetes and the reduction of the occurrence and progression of various 
cancers, the improvement of various cardiovascular risk factors, and the delay of 
osteoporosis and sarcopenia. As for the effects of DR on the nervous system, 
numerous neuroprotective effects have been noted, such as the ability to preserve
cognition, delay brain atrophy, and protect against various neurodegenerative diseases. 
By applying long-term dietary restriction during adulthood in male and female Wistar 
rats, we demonstrated several beneficial results. DR increased the levels of synaptic 
plasticity markers and neurotrophic factors in rat cortex and hippocampus and 
preserved brain cholesterol homeostasis during aging. In addition, it suppressed 
apoptotic cell death after cortical injury and restored age-related impaired 
glucocorticoid receptor signaling (GR) in the brain. DR also demonstrated the ability 
to alter the expression of genes involved in the pathology of AD and to suppress 
microglial activation after cortical injury. Equally important, however, we found that 
DR can also have negative effects depending on the age at which it was introduced 
and the duration of treatment. Namely, we demonstrated that short-term 
administration of DR at older ages had unfavorable effects on cognitive performance, 
anxiety levels, and frailty in Wistar rats. In addition, DR showed some adverse effects 
in transgenic AD animals. The results of our studies urge great caution in the 
introduction of DR in humans, as the feasibility, safety, and adverse effects of DR as 
an anti-aging measure in human populations have not been fully elucidated. To 
achieve a positive effect, it is important to ensure that DR is used in a manner that 
meets the nutritional needs of people of all ages.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Dietary restriction as an anti-aging intervention
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5852
ER  - 

@conference{
author = "Mladenović, Aleksandra and Prvulović, Milica and Milanović, Desanka and Sokanović, Srđan and Vukojević, Anđela and Simeunović, Valentina and Kanazir, Selma and Todorović, Smilja",
year = "2023",
abstract = "Dietary restriction (DR) is considered the gold standard in anti-aging treatment. Since 
the first study on the beneficial effects of DR in 1935, countless research articles have 
demonstrated numerous positive effects of DR. DR is often referred to as the elixir of 
youth due to its proven ability to extend lifespan in various species. The beneficial 
effects of DR are observed in many organ systems, including the prevention of age related diabetes and the reduction of the occurrence and progression of various 
cancers, the improvement of various cardiovascular risk factors, and the delay of 
osteoporosis and sarcopenia. As for the effects of DR on the nervous system, 
numerous neuroprotective effects have been noted, such as the ability to preserve
cognition, delay brain atrophy, and protect against various neurodegenerative diseases. 
By applying long-term dietary restriction during adulthood in male and female Wistar 
rats, we demonstrated several beneficial results. DR increased the levels of synaptic 
plasticity markers and neurotrophic factors in rat cortex and hippocampus and 
preserved brain cholesterol homeostasis during aging. In addition, it suppressed 
apoptotic cell death after cortical injury and restored age-related impaired 
glucocorticoid receptor signaling (GR) in the brain. DR also demonstrated the ability 
to alter the expression of genes involved in the pathology of AD and to suppress 
microglial activation after cortical injury. Equally important, however, we found that 
DR can also have negative effects depending on the age at which it was introduced 
and the duration of treatment. Namely, we demonstrated that short-term 
administration of DR at older ages had unfavorable effects on cognitive performance, 
anxiety levels, and frailty in Wistar rats. In addition, DR showed some adverse effects 
in transgenic AD animals. The results of our studies urge great caution in the 
introduction of DR in humans, as the feasibility, safety, and adverse effects of DR as 
an anti-aging measure in human populations have not been fully elucidated. To 
achieve a positive effect, it is important to ensure that DR is used in a manner that 
meets the nutritional needs of people of all ages.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Dietary restriction as an anti-aging intervention",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5852"
}

Mladenović, A., Prvulović, M., Milanović, D., Sokanović, S., Vukojević, A., Simeunović, V., Kanazir, S.,& Todorović, S.. (2023). Dietary restriction as an anti-aging intervention. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 34.
https://hdl.handle.net/21.15107/rcub_ibiss_5852

Mladenović A, Prvulović M, Milanović D, Sokanović S, Vukojević A, Simeunović V, Kanazir S, Todorović S. Dietary restriction as an anti-aging intervention. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:34.
https://hdl.handle.net/21.15107/rcub_ibiss_5852 .

Mladenović, Aleksandra, Prvulović, Milica, Milanović, Desanka, Sokanović, Srđan, Vukojević, Anđela, Simeunović, Valentina, Kanazir, Selma, Todorović, Smilja, "Dietary restriction as an anti-aging intervention" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):34,
https://hdl.handle.net/21.15107/rcub_ibiss_5852 .

TY  - JOUR
AU  - Prvulović, Milica
AU  - Todorović, Smilja
AU  - Milanović, Desanka
AU  - Jović, Milena
AU  - Vujović, Predrag
AU  - Kanazir, Selma
AU  - Mladenović, Aleksandra
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5303
AB  - Although initially recognized as a universally beneficial approach for the prevention of age-related impairments, the outcome of calorie restriction (CR) is now known to depend on several factors, most notably the age of the subject at the CR commencement, and CR duration. We aimed to examine if and how CR affects anxiety-like behaviour when it is introduced at middle age and late middle age. In addition, as the dopaminergic system is one of the main neurotransmitter systems involved in controlling anxiety, we examined the expression of dopamine receptors (D1R, D2R) in the cortex, striatum, and mesencephalon of male Wistar rats of varying ages. The study was performed on rats fed ad libitum (AL) or exposed to calorie restriction (60% of AL intake). Open field and light-dark tests were used to study anxiety-like behaviour, while PCR and Western blot were used to examine the expression of dopamine receptors. Calorie restriction implemented at middle-age led to variable outcomes on anxiety-like behaviour, while CR implemented at late middle age increased anxiety and decreased the availability of D2R levels in the cortex and mesencephalon. Taken together, these results advise caution when implementing calorie restriction late in life. © 2022
PB  - Elsevier Ireland Ltd
T2  - Mechanisms of Ageing and Development
T1  - Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner
VL  - 204
DO  - 10.1016/j.mad.2022.111666
SP  - 111666
ER  - 

@article{
author = "Prvulović, Milica and Todorović, Smilja and Milanović, Desanka and Jović, Milena and Vujović, Predrag and Kanazir, Selma and Mladenović, Aleksandra",
year = "2022",
abstract = "Although initially recognized as a universally beneficial approach for the prevention of age-related impairments, the outcome of calorie restriction (CR) is now known to depend on several factors, most notably the age of the subject at the CR commencement, and CR duration. We aimed to examine if and how CR affects anxiety-like behaviour when it is introduced at middle age and late middle age. In addition, as the dopaminergic system is one of the main neurotransmitter systems involved in controlling anxiety, we examined the expression of dopamine receptors (D1R, D2R) in the cortex, striatum, and mesencephalon of male Wistar rats of varying ages. The study was performed on rats fed ad libitum (AL) or exposed to calorie restriction (60% of AL intake). Open field and light-dark tests were used to study anxiety-like behaviour, while PCR and Western blot were used to examine the expression of dopamine receptors. Calorie restriction implemented at middle-age led to variable outcomes on anxiety-like behaviour, while CR implemented at late middle age increased anxiety and decreased the availability of D2R levels in the cortex and mesencephalon. Taken together, these results advise caution when implementing calorie restriction late in life. © 2022",
publisher = "Elsevier Ireland Ltd",
journal = "Mechanisms of Ageing and Development",
title = "Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner",
volume = "204",
doi = "10.1016/j.mad.2022.111666",
pages = "111666"
}

Prvulović, M., Todorović, S., Milanović, D., Jović, M., Vujović, P., Kanazir, S.,& Mladenović, A.. (2022). Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner. in Mechanisms of Ageing and Development
Elsevier Ireland Ltd., 204, 111666.
https://doi.org/10.1016/j.mad.2022.111666

Prvulović M, Todorović S, Milanović D, Jović M, Vujović P, Kanazir S, Mladenović A. Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner. in Mechanisms of Ageing and Development. 2022;204:111666.
doi:10.1016/j.mad.2022.111666 .

Prvulović, Milica, Todorović, Smilja, Milanović, Desanka, Jović, Milena, Vujović, Predrag, Kanazir, Selma, Mladenović, Aleksandra, "Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner" in Mechanisms of Ageing and Development, 204 (2022):111666,
https://doi.org/10.1016/j.mad.2022.111666 . .

TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Šobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1756464622003723
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5174
AB  - The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.
T2  - Journal of Functional Foods
T1  - The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice
VL  - 99
DO  - 10.1016/j.jff.2022.105302
SP  - 105302
ER  - 

@article{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Šobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.",
journal = "Journal of Functional Foods",
title = "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice",
volume = "99",
doi = "10.1016/j.jff.2022.105302",
pages = "105302"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Šobajić, S., Kanazir, S.,& Ivković, S.. (2022). The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods, 99, 105302.
https://doi.org/10.1016/j.jff.2022.105302

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Šobajić S, Kanazir S, Ivković S. The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods. 2022;99:105302.
doi:10.1016/j.jff.2022.105302 .

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Šobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice" in Journal of Functional Foods, 99 (2022):105302,
https://doi.org/10.1016/j.jff.2022.105302 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Ivković, Sanja
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9599456
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5172
AB  - Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.
PB  - Basel: MDPI
T2  - Brain Sciences
T1  - Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging
IS  - 10
VL  - 12
DO  - 10.3390/brainsci12101297
SP  - 1297
ER  - 

@article{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Ivković, Sanja and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.",
publisher = "Basel: MDPI",
journal = "Brain Sciences",
title = "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging",
number = "10",
volume = "12",
doi = "10.3390/brainsci12101297",
pages = "1297"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Ivković, S., Kanazir, S.,& Perović, M.. (2022). Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences
Basel: MDPI., 12(10), 1297.
https://doi.org/10.3390/brainsci12101297

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Ivković S, Kanazir S, Perović M. Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences. 2022;12(10):1297.
doi:10.3390/brainsci12101297 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Ivković, Sanja, Kanazir, Selma, Perović, Milka, "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging" in Brain Sciences, 12, no. 10 (2022):1297,
https://doi.org/10.3390/brainsci12101297 . .

TY  - JOUR
AU  - Dominko, Kristina
AU  - Rastija, Ana
AU  - Smiljanić, Kosara
AU  - Mladenović, Aleksandra
AU  - Lešnjaković, Lucija
AU  - Kanazir, Selma
AU  - Milanović, Desanka
AU  - Hećimović, Silva
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0047637422001087
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5109
AB  - The formation of amyloid-ß peptides (Aß), that accumulate in Alzheimer's disease (AD) brains, involves proteolytic processing of the amyloid precursor protein (APP) firstly by ß-secretase (BACE1). Since BACE1 cleaves a plethora of other substrates, in this work we investigated whether the proteolysis and/or distribution of other BACE1 substrates, such as seizure protein 6 (Sez6) and seizure 6-like protein (Sez6L), is altered in AD. To test this we used 5xFAD mouse model brains that show an early accumulation of Aß plaques already at 2-months of age. Here we show for the first time that accumulation of BACE1 in peri-plaque regions and its enhanced levels in AD brains does not affect proteolysis of BACE1 substrates other than APP, such as Sez6 and Sez6L. We observed altered distribution of Sez6 and Sez6L in the area of Aß plaques in 5xFAD brains which is distinct to that of APP, BACE1 and/or LAMP1, suggesting different localization and/or function of these BACE1 substrates. While it is necessary to further elucidate the potential role that this may play in the course of AD, it is likely that Aß-targeted therapies may have beneficial effects against accumulation and/or altered distribution of BACE1 and its substrates, in addition to APP.
PB  - Clare: Elsevier Ireland Ltd.
T2  - Mechanisms of Ageing and Development
T1  - Amyloid-ß plaque formation and BACE1 accumulation in the brains of a 5xFAD Alzheimer's disease mouse model is associated with altered distribution and not proteolysis of BACE1 substrates Sez6 and Sez6L.
VL  - 207
DO  - 10.1016/j.mad.2022.111726
SP  - 111726
ER  - 

@article{
author = "Dominko, Kristina and Rastija, Ana and Smiljanić, Kosara and Mladenović, Aleksandra and Lešnjaković, Lucija and Kanazir, Selma and Milanović, Desanka and Hećimović, Silva",
year = "2022",
abstract = "The formation of amyloid-ß peptides (Aß), that accumulate in Alzheimer's disease (AD) brains, involves proteolytic processing of the amyloid precursor protein (APP) firstly by ß-secretase (BACE1). Since BACE1 cleaves a plethora of other substrates, in this work we investigated whether the proteolysis and/or distribution of other BACE1 substrates, such as seizure protein 6 (Sez6) and seizure 6-like protein (Sez6L), is altered in AD. To test this we used 5xFAD mouse model brains that show an early accumulation of Aß plaques already at 2-months of age. Here we show for the first time that accumulation of BACE1 in peri-plaque regions and its enhanced levels in AD brains does not affect proteolysis of BACE1 substrates other than APP, such as Sez6 and Sez6L. We observed altered distribution of Sez6 and Sez6L in the area of Aß plaques in 5xFAD brains which is distinct to that of APP, BACE1 and/or LAMP1, suggesting different localization and/or function of these BACE1 substrates. While it is necessary to further elucidate the potential role that this may play in the course of AD, it is likely that Aß-targeted therapies may have beneficial effects against accumulation and/or altered distribution of BACE1 and its substrates, in addition to APP.",
publisher = "Clare: Elsevier Ireland Ltd.",
journal = "Mechanisms of Ageing and Development",
title = "Amyloid-ß plaque formation and BACE1 accumulation in the brains of a 5xFAD Alzheimer's disease mouse model is associated with altered distribution and not proteolysis of BACE1 substrates Sez6 and Sez6L.",
volume = "207",
doi = "10.1016/j.mad.2022.111726",
pages = "111726"
}

Dominko, K., Rastija, A., Smiljanić, K., Mladenović, A., Lešnjaković, L., Kanazir, S., Milanović, D.,& Hećimović, S.. (2022). Amyloid-ß plaque formation and BACE1 accumulation in the brains of a 5xFAD Alzheimer's disease mouse model is associated with altered distribution and not proteolysis of BACE1 substrates Sez6 and Sez6L.. in Mechanisms of Ageing and Development
Clare: Elsevier Ireland Ltd.., 207, 111726.
https://doi.org/10.1016/j.mad.2022.111726

Dominko K, Rastija A, Smiljanić K, Mladenović A, Lešnjaković L, Kanazir S, Milanović D, Hećimović S. Amyloid-ß plaque formation and BACE1 accumulation in the brains of a 5xFAD Alzheimer's disease mouse model is associated with altered distribution and not proteolysis of BACE1 substrates Sez6 and Sez6L.. in Mechanisms of Ageing and Development. 2022;207:111726.
doi:10.1016/j.mad.2022.111726 .

Dominko, Kristina, Rastija, Ana, Smiljanić, Kosara, Mladenović, Aleksandra, Lešnjaković, Lucija, Kanazir, Selma, Milanović, Desanka, Hećimović, Silva, "Amyloid-ß plaque formation and BACE1 accumulation in the brains of a 5xFAD Alzheimer's disease mouse model is associated with altered distribution and not proteolysis of BACE1 substrates Sez6 and Sez6L." in Mechanisms of Ageing and Development, 207 (2022):111726,
https://doi.org/10.1016/j.mad.2022.111726 . .

TY  - JOUR
AU  - Prvulović, Milica
AU  - Todorović, Smilja
AU  - Milanović, Desanka
AU  - Jović, Milena
AU  - Vujović, Predrag
AU  - Kanazir, Selma
AU  - Mladenović, Aleksandra
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0047637422000483
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4946
AB  - Although initially recognized as a universally beneficial approach for the prevention of age-related impairments, the outcome of calorie restriction (CR) is now known to depend on several factors, most notably the age of the subject at the CR commencement, and CR duration. We aimed to examine if and how CR affects anxiety-like behaviour when it is introduced at middle age and late middle age. In addition, as the dopaminergic system is one of the main neurotransmitter systems involved in controlling anxiety, we examined the expression of dopamine receptors (D1R, D2R) in the cortex, striatum, and mesencephalon of male Wistar rats of varying ages. The study was performed on rats fed ad libitum (AL) or exposed to calorie restriction (60% of AL intake). Open field and light-dark tests were used to study anxiety-like behaviour, while PCR and Western blot were used to examine the expression of dopamine receptors. Calorie restriction implemented at middle-age led to variable outcomes on anxiety-like behaviour, while CR implemented at late middle age increased anxiety and decreased the availability of D2R levels in the cortex and mesencephalon. Taken together, these results advise caution when implementing calorie restriction late in life.
PB  - Elsevier Ireland Ltd
T2  - Mechanisms of Ageing and Development
T1  - Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner.
VL  - 204
DO  - 10.1016/j.mad.2022.111666
SP  - 111666
ER  - 

@article{
author = "Prvulović, Milica and Todorović, Smilja and Milanović, Desanka and Jović, Milena and Vujović, Predrag and Kanazir, Selma and Mladenović, Aleksandra",
year = "2022",
abstract = "Although initially recognized as a universally beneficial approach for the prevention of age-related impairments, the outcome of calorie restriction (CR) is now known to depend on several factors, most notably the age of the subject at the CR commencement, and CR duration. We aimed to examine if and how CR affects anxiety-like behaviour when it is introduced at middle age and late middle age. In addition, as the dopaminergic system is one of the main neurotransmitter systems involved in controlling anxiety, we examined the expression of dopamine receptors (D1R, D2R) in the cortex, striatum, and mesencephalon of male Wistar rats of varying ages. The study was performed on rats fed ad libitum (AL) or exposed to calorie restriction (60% of AL intake). Open field and light-dark tests were used to study anxiety-like behaviour, while PCR and Western blot were used to examine the expression of dopamine receptors. Calorie restriction implemented at middle-age led to variable outcomes on anxiety-like behaviour, while CR implemented at late middle age increased anxiety and decreased the availability of D2R levels in the cortex and mesencephalon. Taken together, these results advise caution when implementing calorie restriction late in life.",
publisher = "Elsevier Ireland Ltd",
journal = "Mechanisms of Ageing and Development",
title = "Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner.",
volume = "204",
doi = "10.1016/j.mad.2022.111666",
pages = "111666"
}

Prvulović, M., Todorović, S., Milanović, D., Jović, M., Vujović, P., Kanazir, S.,& Mladenović, A.. (2022). Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner.. in Mechanisms of Ageing and Development
Elsevier Ireland Ltd., 204, 111666.
https://doi.org/10.1016/j.mad.2022.111666

Prvulović M, Todorović S, Milanović D, Jović M, Vujović P, Kanazir S, Mladenović A. Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner.. in Mechanisms of Ageing and Development. 2022;204:111666.
doi:10.1016/j.mad.2022.111666 .

Prvulović, Milica, Todorović, Smilja, Milanović, Desanka, Jović, Milena, Vujović, Predrag, Kanazir, Selma, Mladenović, Aleksandra, "Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner." in Mechanisms of Ageing and Development, 204 (2022):111666,
https://doi.org/10.1016/j.mad.2022.111666 . .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Hofman, Katarina
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5613
AB  - Дуготрајна рестрикција уноса хране повољно делује на организам у целини, а 
показанa су и бројна неуропротективна дејства овог режима исхране на обољења 
повезана са старењем, попут Алцхајмерове болести. Циљ ове студије је био да се 
испита превентивно дејство рестрикције уноса хране и утицај на парвалбуминске 
неуроне (PV) коре великог мозга и BDNF/ТrkB сигнални пут у трансгеном моделу 
Алцхајмерове болести. Женке 5XFAD мишева и њихове не-трансгене контроле су 
биле излагане ad libidum (AL) или EOD (од енгл. Every-Other-Day feeding) режиму 
исхране почевши од другог месеца старости. Број PV неурона је одређиван 
имунохистохемијском методом у  retrosplenial dysgranular cortex  (RSD), 
retrosplenial granular cortex  (RSG),  parietal cortex  (PtA) и  somatosensory  cortex  (S) 
код животиња старих шест месеци. Код TgAL мишева је утврђено значајно 
смањење броја PV неурона у RSGc, PtA и S, док промене у броју у RSD нису 
уочене. Четири месеца ЕОD режима исхране је смањило пад у броју PV неурона у 
сва три испитивана региона. Анализа BDNF/ТrkB сигналног пута имуноблот 
поступком је такође указала на смањење BDNF-а код TgAL мишева, али и на 
додатно смањење овог протеина код TgЕОD мишева. Значајне разлике у pro-
BDNF-у (прекурсор  BDNF-а)  нису уочене. Резултати ове студије указују да 
рестрикција хране може спречити губитак PV неурона у трансгеном моделу 
Алцхајмерове болести, што доприноси и бољем разумевању неуронске  основе 
когнитивних поремећаја у овом обољењу и од значаја је за даљи развој потребних 
додатних терапијских приступа.
AB  - Dugotrajna restrikcija unosa hrane povoljno deluje na organizam u celini, a pokazana su i brojna neuroprotektivna dejstva ovog režima ishrane na oboljenja povezana sa starenjem, poput Alchajmerove bolesti. Cilj ove studije je bio da se ispita preventivno dejstvo restrikcije unosa hrane i uticaj na parvalbuminske neurone (PV) kore velikog mozga i BDNF/TrkB signalni put u transgenom modelu Alchajmerove bolesti. Ženke 5XFAD miševa i njihove ne-transgene kontrole su bile izlagane ad libidum (AL) ili EOD (od engl. Every-Other-Day feeding) režimu ishrane počevši od drugog meseca starosti. Broj PV neurona je određivan imunohistohemijskom metodom u retrosplenial dysgranular cortex (RSD), retrosplenial granular cortex (RSG), parietal cortex (PtA) i somatosensory cortex (S) kod životinja starih šest meseci. Kod TgAL miševa je utvrđeno značajno smanjenje broja PV neurona u RSGc, PtA i S, dok promene u broju u RSD nisu uočene. Četiri meseca EOD režima ishrane je smanjilo pad u broju PV neurona u sva tri ispitivana regiona. Analiza BDNF/TrkB signalnog puta imunoblot postupkom je takođe ukazala na smanjenje BDNF-a kod TgAL miševa, ali i na dodatno smanjenje ovog proteina kod TgEOD miševa. Značajne razlike u pro- BDNF-u (prekursor BDNF-a) nisu uočene. Rezultati ove studije ukazuju da restrikcija hrane može sprečiti gubitak PV neurona u transgenom modelu Alchajmerove bolesti, što doprinosi i boljem razumevanju neuronske osnove kognitivnih poremećaja u ovom oboljenju i od značaja je za dalji razvoj potrebnih dodatnih terapijskih pristupa.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti
T1  - Дејство рестрикције хране на парвалбуминске неуроне коре великог мозга у трансгеном моделу Алцхајмерове болести
SP  - 343
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5613
ER  - 

@conference{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Hofman, Katarina and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Дуготрајна рестрикција уноса хране повољно делује на организам у целини, а 
показанa су и бројна неуропротективна дејства овог режима исхране на обољења 
повезана са старењем, попут Алцхајмерове болести. Циљ ове студије је био да се 
испита превентивно дејство рестрикције уноса хране и утицај на парвалбуминске 
неуроне (PV) коре великог мозга и BDNF/ТrkB сигнални пут у трансгеном моделу 
Алцхајмерове болести. Женке 5XFAD мишева и њихове не-трансгене контроле су 
биле излагане ad libidum (AL) или EOD (од енгл. Every-Other-Day feeding) режиму 
исхране почевши од другог месеца старости. Број PV неурона је одређиван 
имунохистохемијском методом у  retrosplenial dysgranular cortex  (RSD), 
retrosplenial granular cortex  (RSG),  parietal cortex  (PtA) и  somatosensory  cortex  (S) 
код животиња старих шест месеци. Код TgAL мишева је утврђено значајно 
смањење броја PV неурона у RSGc, PtA и S, док промене у броју у RSD нису 
уочене. Четири месеца ЕОD режима исхране је смањило пад у броју PV неурона у 
сва три испитивана региона. Анализа BDNF/ТrkB сигналног пута имуноблот 
поступком је такође указала на смањење BDNF-а код TgAL мишева, али и на 
додатно смањење овог протеина код TgЕОD мишева. Значајне разлике у pro-
BDNF-у (прекурсор  BDNF-а)  нису уочене. Резултати ове студије указују да 
рестрикција хране може спречити губитак PV неурона у трансгеном моделу 
Алцхајмерове болести, што доприноси и бољем разумевању неуронске  основе 
когнитивних поремећаја у овом обољењу и од значаја је за даљи развој потребних 
додатних терапијских приступа., Dugotrajna restrikcija unosa hrane povoljno deluje na organizam u celini, a pokazana su i brojna neuroprotektivna dejstva ovog režima ishrane na oboljenja povezana sa starenjem, poput Alchajmerove bolesti. Cilj ove studije je bio da se ispita preventivno dejstvo restrikcije unosa hrane i uticaj na parvalbuminske neurone (PV) kore velikog mozga i BDNF/TrkB signalni put u transgenom modelu Alchajmerove bolesti. Ženke 5XFAD miševa i njihove ne-transgene kontrole su bile izlagane ad libidum (AL) ili EOD (od engl. Every-Other-Day feeding) režimu ishrane počevši od drugog meseca starosti. Broj PV neurona je određivan imunohistohemijskom metodom u retrosplenial dysgranular cortex (RSD), retrosplenial granular cortex (RSG), parietal cortex (PtA) i somatosensory cortex (S) kod životinja starih šest meseci. Kod TgAL miševa je utvrđeno značajno smanjenje broja PV neurona u RSGc, PtA i S, dok promene u broju u RSD nisu uočene. Četiri meseca EOD režima ishrane je smanjilo pad u broju PV neurona u sva tri ispitivana regiona. Analiza BDNF/TrkB signalnog puta imunoblot postupkom je takođe ukazala na smanjenje BDNF-a kod TgAL miševa, ali i na dodatno smanjenje ovog proteina kod TgEOD miševa. Značajne razlike u pro- BDNF-u (prekursor BDNF-a) nisu uočene. Rezultati ove studije ukazuju da restrikcija hrane može sprečiti gubitak PV neurona u transgenom modelu Alchajmerove bolesti, što doprinosi i boljem razumevanju neuronske osnove kognitivnih poremećaja u ovom oboljenju i od značaja je za dalji razvoj potrebnih dodatnih terapijskih pristupa.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti, Дејство рестрикције хране на парвалбуминске неуроне коре великог мозга у трансгеном моделу Алцхајмерове болести",
pages = "343",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5613"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Hofman, K., Kanazir, S.,& Perović, M.. (2022). Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 343.
https://hdl.handle.net/21.15107/rcub_ibiss_5613

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Hofman K, Kanazir S, Perović M. Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:343.
https://hdl.handle.net/21.15107/rcub_ibiss_5613 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Hofman, Katarina, Kanazir, Selma, Perović, Milka, "Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):343,
https://hdl.handle.net/21.15107/rcub_ibiss_5613 .

TY  - CONF
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5615
AB  - Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење и 
најчешћи узрок деменције код старих особа, са преваленцом два пута већом у 
женској популацији.  Упркос дугогодишњим истраживањима механизама 
патогенезе АБ, као и бројним спроведеним претклиничким студијама, још увек не 
постоји адекватна терапија за ово обољење. У овој иницијалној студији испитиван 
је неуропротективни потенцијал нано квантне супстанце 3HFWC  –  хипер-
хармонизованог  комплекса  хидроксилованог фулерена и воде, у  животињском 
моделу АБ  –  трансгеним  5XFAD мишевима. Женке 5XFAD мишева  су  излагане 
нано квантној супстанци 3HFWC у продромалној фази патологије. Третман је 
започет када су животиње биле старе 4 недеље и животиње су појене раствором 
нано квантне  супстанце 3HFWC  уместо воде током наредна три месеца.  Након 
третмана, анализирани су број и морфолошке карактеристике амилоидних плакова 
у структурама мозга од значаја за процесе учења и памћења – кори великог мозга и 
хипокампусу. Испитиван је и ефекат третмана на акумулацију токсичног протеина 
амилоида бета (Аβ), као и промене у маркерима синаптичке пластичности. Третман 
са 3HFWC је значајно смањио заступљеност амилоидних плакова у одређеним 
регионима коре великог мозга. Резултати стога указују  на неуропротективно 
дејство превентивне примене  нано  квантне  супстанце 3HFWC  у  мишјем моделу 
Алцхајмерове болести.
AB  - Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje i najčešći uzrok demencije kod starih osoba, sa prevalencom dva puta većom u ženskoj populaciji. Uprkos dugogodišnjim istraživanjima mehanizama patogeneze AB, kao i brojnim sprovedenim pretkliničkim studijama, još uvek ne postoji adekvatna terapija za ovo oboljenje. U ovoj inicijalnoj studiji ispitivan je neuroprotektivni potencijal nano kvantne supstance 3HFWC – hiper- harmonizovanog kompleksa hidroksilovanog fulerena i vode, u životinjskom modelu AB – transgenim 5XFAD miševima. Ženke 5XFAD miševa su izlagane nano kvantnoj supstanci 3HFWC u prodromalnoj fazi patologije. Tretman je započet kada su životinje bile stare 4 nedelje i životinje su pojene rastvorom nano kvantne supstance 3HFWC umesto vode tokom naredna tri meseca. Nakon tretmana, analizirani su broj i morfološke karakteristike amiloidnih plakova u strukturama mozga od značaja za procese učenja i pamćenja – kori velikog mozga i hipokampusu. Ispitivan je i efekat tretmana na akumulaciju toksičnog proteina amiloida beta (Aβ), kao i promene u markerima sinaptičke plastičnosti. Tretman sa 3HFWC je značajno smanjio zastupljenost amiloidnih plakova u određenim regionima kore velikog mozga. Rezultati stoga ukazuju na neuroprotektivno dejstvo preventivne primene nano kvantne supstance 3HFWC u mišjem modelu Alchajmerove bolesti.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti
T1  - Испитивање неуропротективног потенцијала наноквантне супстанце 3HFWC у мишијем моделу Алцхајмерове болести
SP  - 317
SP  - M64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5615
ER  - 

@conference{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење и 
најчешћи узрок деменције код старих особа, са преваленцом два пута већом у 
женској популацији.  Упркос дугогодишњим истраживањима механизама 
патогенезе АБ, као и бројним спроведеним претклиничким студијама, још увек не 
постоји адекватна терапија за ово обољење. У овој иницијалној студији испитиван 
је неуропротективни потенцијал нано квантне супстанце 3HFWC  –  хипер-
хармонизованог  комплекса  хидроксилованог фулерена и воде, у  животињском 
моделу АБ  –  трансгеним  5XFAD мишевима. Женке 5XFAD мишева  су  излагане 
нано квантној супстанци 3HFWC у продромалној фази патологије. Третман је 
започет када су животиње биле старе 4 недеље и животиње су појене раствором 
нано квантне  супстанце 3HFWC  уместо воде током наредна три месеца.  Након 
третмана, анализирани су број и морфолошке карактеристике амилоидних плакова 
у структурама мозга од значаја за процесе учења и памћења – кори великог мозга и 
хипокампусу. Испитиван је и ефекат третмана на акумулацију токсичног протеина 
амилоида бета (Аβ), као и промене у маркерима синаптичке пластичности. Третман 
са 3HFWC је значајно смањио заступљеност амилоидних плакова у одређеним 
регионима коре великог мозга. Резултати стога указују  на неуропротективно 
дејство превентивне примене  нано  квантне  супстанце 3HFWC  у  мишјем моделу 
Алцхајмерове болести., Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje i najčešći uzrok demencije kod starih osoba, sa prevalencom dva puta većom u ženskoj populaciji. Uprkos dugogodišnjim istraživanjima mehanizama patogeneze AB, kao i brojnim sprovedenim pretkliničkim studijama, još uvek ne postoji adekvatna terapija za ovo oboljenje. U ovoj inicijalnoj studiji ispitivan je neuroprotektivni potencijal nano kvantne supstance 3HFWC – hiper- harmonizovanog kompleksa hidroksilovanog fulerena i vode, u životinjskom modelu AB – transgenim 5XFAD miševima. Ženke 5XFAD miševa su izlagane nano kvantnoj supstanci 3HFWC u prodromalnoj fazi patologije. Tretman je započet kada su životinje bile stare 4 nedelje i životinje su pojene rastvorom nano kvantne supstance 3HFWC umesto vode tokom naredna tri meseca. Nakon tretmana, analizirani su broj i morfološke karakteristike amiloidnih plakova u strukturama mozga od značaja za procese učenja i pamćenja – kori velikog mozga i hipokampusu. Ispitivan je i efekat tretmana na akumulaciju toksičnog proteina amiloida beta (Aβ), kao i promene u markerima sinaptičke plastičnosti. Tretman sa 3HFWC je značajno smanjio zastupljenost amiloidnih plakova u određenim regionima kore velikog mozga. Rezultati stoga ukazuju na neuroprotektivno dejstvo preventivne primene nano kvantne supstance 3HFWC u mišjem modelu Alchajmerove bolesti.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti, Испитивање неуропротективног потенцијала наноквантне супстанце 3HFWC у мишијем моделу Алцхајмерове болести",
pages = "317-M64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5615"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2022). Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 317.
https://hdl.handle.net/21.15107/rcub_ibiss_5615

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:317.
https://hdl.handle.net/21.15107/rcub_ibiss_5615 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):317,
https://hdl.handle.net/21.15107/rcub_ibiss_5615 .

TY  - CONF
AU  - Todorović, Smilja
AU  - Prvulović, Milica
AU  - Milanović, Desanka
AU  - Sokanović, Srđan
AU  - Vukojević, Anđela
AU  - Simeunović, Valentina
AU  - Kanazir, Selma
AU  - Mladenović, Aleksandra
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5322
AB  - Dietary restriction (DR) is the oldest and the most investigated anti­aging intervention. Since the famous McCay's study that showed that the restriction of calories
without malnutrition prolongs mean and maximal lifespan in rats, thousands of papers demonstrated numerous beneficial effects of DR. DR is most famous for its proven
capability to prolong life span, but what we know today is that DR prevents age­related diabetes, it decreases both incidence and progression of the different forms of
cancer, protects from cardiovascular diseases, delays osteoporosis and sarcopenia. Its effects on the nervous system include preserved cognition, delayed brain atrophy,
and protection from various neurodegenerative diseases. 
By applying long­term dietary restrictions from the adulthood of male and female Wistar rats we demonstrated several beneficial outcomes. DR increased the level of
synaptic plasticity markers and neurotrophic factors in the rat cortex and hippocampus and preserved brain cholesterol homeostasis during aging. It also suppressed
apoptotic cell deaths after cortical injury and restored age­related impaired glucocorticoid receptor (GR) signalling in the brain. DR changed the expression of genes
involved in AD pathology and suppressed microglial activation following cortical injury. However, recently we have been shown that the outcome of DR is highly
dependent on the onset and duration. Namely, we demonstrated that short–term DR with a late­onset could have unfavorable effects on cognitive performances, anxiety
level, and frailty in Wistar rats. In addition, DR could have a negative impact when introduced to transgenic AD animals. The results of our studies impose great caution
when introducing CR to humans. To achieve its favorable effect DR should be introduced in humans up to the middle age.
PB  - Federation of European Biochemical Societies
C3  - Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece
T1  - Dietary restriction as an antiaging intervention
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5322
ER  - 

@conference{
author = "Todorović, Smilja and Prvulović, Milica and Milanović, Desanka and Sokanović, Srđan and Vukojević, Anđela and Simeunović, Valentina and Kanazir, Selma and Mladenović, Aleksandra",
year = "2022",
abstract = "Dietary restriction (DR) is the oldest and the most investigated anti­aging intervention. Since the famous McCay's study that showed that the restriction of calories
without malnutrition prolongs mean and maximal lifespan in rats, thousands of papers demonstrated numerous beneficial effects of DR. DR is most famous for its proven
capability to prolong life span, but what we know today is that DR prevents age­related diabetes, it decreases both incidence and progression of the different forms of
cancer, protects from cardiovascular diseases, delays osteoporosis and sarcopenia. Its effects on the nervous system include preserved cognition, delayed brain atrophy,
and protection from various neurodegenerative diseases. 
By applying long­term dietary restrictions from the adulthood of male and female Wistar rats we demonstrated several beneficial outcomes. DR increased the level of
synaptic plasticity markers and neurotrophic factors in the rat cortex and hippocampus and preserved brain cholesterol homeostasis during aging. It also suppressed
apoptotic cell deaths after cortical injury and restored age­related impaired glucocorticoid receptor (GR) signalling in the brain. DR changed the expression of genes
involved in AD pathology and suppressed microglial activation following cortical injury. However, recently we have been shown that the outcome of DR is highly
dependent on the onset and duration. Namely, we demonstrated that short–term DR with a late­onset could have unfavorable effects on cognitive performances, anxiety
level, and frailty in Wistar rats. In addition, DR could have a negative impact when introduced to transgenic AD animals. The results of our studies impose great caution
when introducing CR to humans. To achieve its favorable effect DR should be introduced in humans up to the middle age.",
publisher = "Federation of European Biochemical Societies",
journal = "Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece",
title = "Dietary restriction as an antiaging intervention",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5322"
}

Todorović, S., Prvulović, M., Milanović, D., Sokanović, S., Vukojević, A., Simeunović, V., Kanazir, S.,& Mladenović, A.. (2022). Dietary restriction as an antiaging intervention. in Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece
Federation of European Biochemical Societies..
https://hdl.handle.net/21.15107/rcub_ibiss_5322

Todorović S, Prvulović M, Milanović D, Sokanović S, Vukojević A, Simeunović V, Kanazir S, Mladenović A. Dietary restriction as an antiaging intervention. in Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5322 .

Todorović, Smilja, Prvulović, Milica, Milanović, Desanka, Sokanović, Srđan, Vukojević, Anđela, Simeunović, Valentina, Kanazir, Selma, Mladenović, Aleksandra, "Dietary restriction as an antiaging intervention" in Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5322 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5187
AB  - Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice
DO  - 10.1002/2211-5463.13440
SP  - 132
ER  - 

@conference{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice",
doi = "10.1002/2211-5463.13440",
pages = "132"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Kanazir, S.,& Perović, M.. (2022). Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 132.
https://doi.org/10.1002/2211-5463.13440

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Kanazir S, Perović M. Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:132.
doi:10.1002/2211-5463.13440 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Kanazir, Selma, Perović, Milka, "Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):132,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - CONF
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5188
AB  - The potential of fullerenes and fullerene’s water-soluble derivatives
to bind to amyloid-b has been well documented in vitro and
in silico. However, the anti-amyloid action of fullerenols in
in vivo treatments has not been fully examined. In the present study we assessed the effects of the hydroxylated fullerene-water
complex (3HFWC) on Alzheimer’s disease (AD) neuropathological
hallmarks in 5XFAD mice, a well-recognized AD animal
model. The 3-week-old 5XFAD mice were exposed to 3HFWC
water solution ad libitum for 3 months. The 3HFWC treatment
started in the presymptomatic phase of pathology and analyses
were focused on the effects on amyloid-b (Ab) accumulation, plaque
formation, gliosis, and synaptic plasticity in cortical and hippocampal
tissue. The 3HFWC treatment significantly decreased
the amyloid-b plaque load in specific parts of cerebral cortex, followed
by the unchanged levels of Ab. None of these changes
were detected in the hippocampus. At the same time, 3HFWC
treatment did not exacerbate the activation of glial cells, nor
altered the expression levels of synaptic proteins. The obtained
results point to the potential of 3HFWC, when applied in the
presymptomatic phase of AD, to interfere with Ab accumulation
and amyloid plaque formation without exacerbating the other
AD-related pathological processes.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease
DO  - 10.1002/2211-5463.13440
SP  - 130
ER  - 

@conference{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "The potential of fullerenes and fullerene’s water-soluble derivatives
to bind to amyloid-b has been well documented in vitro and
in silico. However, the anti-amyloid action of fullerenols in
in vivo treatments has not been fully examined. In the present study we assessed the effects of the hydroxylated fullerene-water
complex (3HFWC) on Alzheimer’s disease (AD) neuropathological
hallmarks in 5XFAD mice, a well-recognized AD animal
model. The 3-week-old 5XFAD mice were exposed to 3HFWC
water solution ad libitum for 3 months. The 3HFWC treatment
started in the presymptomatic phase of pathology and analyses
were focused on the effects on amyloid-b (Ab) accumulation, plaque
formation, gliosis, and synaptic plasticity in cortical and hippocampal
tissue. The 3HFWC treatment significantly decreased
the amyloid-b plaque load in specific parts of cerebral cortex, followed
by the unchanged levels of Ab. None of these changes
were detected in the hippocampus. At the same time, 3HFWC
treatment did not exacerbate the activation of glial cells, nor
altered the expression levels of synaptic proteins. The obtained
results point to the potential of 3HFWC, when applied in the
presymptomatic phase of AD, to interfere with Ab accumulation
and amyloid plaque formation without exacerbating the other
AD-related pathological processes.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease",
doi = "10.1002/2211-5463.13440",
pages = "130"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2022). Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 130.
https://doi.org/10.1002/2211-5463.13440

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:130.
doi:10.1002/2211-5463.13440 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):130,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - JOUR
AU  - Prvulović, Milica
AU  - Milanović, Desanka
AU  - Vujović, Predrag
AU  - Jović, Milena
AU  - Kanazir, Selma
AU  - Todorović, Smilja
AU  - Mladenović, Aleksandra
PY  - 2021
UR  - https://academic.oup.com/biomedgerontology/advance-article/doi/10.1093/gerona/glab353/6433769
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5436
AB  - The current study aims to determine the potential benefits of calorie restriction (CR), one of the most promising paradigms for life span and healthspan extension, on cognitive performances in female Wistar rats during aging. As a measure of a healthspan, we evaluated the effects of different onset and duration of CR on frailty level. Female Wistar rats were exposed to either ad libitum (AL) or CR (60% of AL daily intake) food intake during aging. Two different CR protocols were used, life-long CR with an early-onset that started at the adult stage (6 months) and 3-month-long CR, started at the middle (15 months) and late-middle (21 months) age, thus defined as a late-onset CR. The effects of CR were evaluated using open-field, Y-maze, and novel object recognition tests. We broadened 2 tools for frailty assessment currently in use for experimental animals, and in alignment with our previous study, we created a physical-cognitive frailty tool that combines both physical and cognitive performances. Our results clearly showed that CR effects are highly dependent on CR duration and onset. While a life-long restriction with an early-onset has been proven as protective and beneficial, short-term restriction introduced at late age significantly worsens an animal's behavior and frailty. These results complement our previous study conducted in males and contribute to the understanding of sex differences in a response to CR during aging.
T2  - The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences
T1  - Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.
IS  - 5
VL  - 77
DO  - 10.1093/gerona/glab353
SP  - glab353
SP  - 947
EP  - 955
ER  - 

@article{
author = "Prvulović, Milica and Milanović, Desanka and Vujović, Predrag and Jović, Milena and Kanazir, Selma and Todorović, Smilja and Mladenović, Aleksandra",
year = "2021",
abstract = "The current study aims to determine the potential benefits of calorie restriction (CR), one of the most promising paradigms for life span and healthspan extension, on cognitive performances in female Wistar rats during aging. As a measure of a healthspan, we evaluated the effects of different onset and duration of CR on frailty level. Female Wistar rats were exposed to either ad libitum (AL) or CR (60% of AL daily intake) food intake during aging. Two different CR protocols were used, life-long CR with an early-onset that started at the adult stage (6 months) and 3-month-long CR, started at the middle (15 months) and late-middle (21 months) age, thus defined as a late-onset CR. The effects of CR were evaluated using open-field, Y-maze, and novel object recognition tests. We broadened 2 tools for frailty assessment currently in use for experimental animals, and in alignment with our previous study, we created a physical-cognitive frailty tool that combines both physical and cognitive performances. Our results clearly showed that CR effects are highly dependent on CR duration and onset. While a life-long restriction with an early-onset has been proven as protective and beneficial, short-term restriction introduced at late age significantly worsens an animal's behavior and frailty. These results complement our previous study conducted in males and contribute to the understanding of sex differences in a response to CR during aging.",
journal = "The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences",
title = "Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.",
number = "5",
volume = "77",
doi = "10.1093/gerona/glab353",
pages = "glab353-947-955"
}

Prvulović, M., Milanović, D., Vujović, P., Jović, M., Kanazir, S., Todorović, S.,& Mladenović, A.. (2021). Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.. in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 77(5), glab353-955.
https://doi.org/10.1093/gerona/glab353

Prvulović M, Milanović D, Vujović P, Jović M, Kanazir S, Todorović S, Mladenović A. Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.. in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences. 2021;77(5):glab353-955.
doi:10.1093/gerona/glab353 .

Prvulović, Milica, Milanović, Desanka, Vujović, Predrag, Jović, Milena, Kanazir, Selma, Todorović, Smilja, Mladenović, Aleksandra, "Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats." in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 77, no. 5 (2021):glab353-955,
https://doi.org/10.1093/gerona/glab353 . .

TY  - JOUR
AU  - Prvulović, Milica
AU  - Milanović, Desanka
AU  - Vujović, Predrag
AU  - Jović, Milena
AU  - Kanazir, Selma
AU  - Todorović, Smilja
AU  - Mladenović, Aleksandra
PY  - 2021
UR  - https://academic.oup.com/biomedgerontology/advance-article/doi/10.1093/gerona/glab353/6433769
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4774
AB  - The current study aims to determine the potential benefits of calorie restriction (CR), one of the most promising paradigms for life span and healthspan extension, on cognitive performances in female Wistar rats during aging. As a measure of a healthspan, we evaluated the effects of different onset and duration of CR on frailty level. Female Wistar rats were exposed to either ad libitum (AL) or CR (60% of AL daily intake) food intake during aging. Two different CR protocols were used, life-long CR with an early-onset that started at the adult stage (6 months) and 3-month-long CR, started at the middle (15 months) and late-middle (21 months) age, thus defined as a late-onset CR. The effects of CR were evaluated using open-field, Y-maze, and novel object recognition tests. We broadened 2 tools for frailty assessment currently in use for experimental animals, and in alignment with our previous study, we created a physical-cognitive frailty tool that combines both physical and cognitive performances. Our results clearly showed that CR effects are highly dependent on CR duration and onset. While a life-long restriction with an early-onset has been proven as protective and beneficial, short-term restriction introduced at late age significantly worsens an animal's behavior and frailty. These results complement our previous study conducted in males and contribute to the understanding of sex differences in a response to CR during aging.
T2  - The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences
T1  - Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.
IS  - 5
VL  - 77
DO  - 10.1093/gerona/glab353
SP  - glab353
SP  - 947
EP  - 955
ER  - 

@article{
author = "Prvulović, Milica and Milanović, Desanka and Vujović, Predrag and Jović, Milena and Kanazir, Selma and Todorović, Smilja and Mladenović, Aleksandra",
year = "2021",
abstract = "The current study aims to determine the potential benefits of calorie restriction (CR), one of the most promising paradigms for life span and healthspan extension, on cognitive performances in female Wistar rats during aging. As a measure of a healthspan, we evaluated the effects of different onset and duration of CR on frailty level. Female Wistar rats were exposed to either ad libitum (AL) or CR (60% of AL daily intake) food intake during aging. Two different CR protocols were used, life-long CR with an early-onset that started at the adult stage (6 months) and 3-month-long CR, started at the middle (15 months) and late-middle (21 months) age, thus defined as a late-onset CR. The effects of CR were evaluated using open-field, Y-maze, and novel object recognition tests. We broadened 2 tools for frailty assessment currently in use for experimental animals, and in alignment with our previous study, we created a physical-cognitive frailty tool that combines both physical and cognitive performances. Our results clearly showed that CR effects are highly dependent on CR duration and onset. While a life-long restriction with an early-onset has been proven as protective and beneficial, short-term restriction introduced at late age significantly worsens an animal's behavior and frailty. These results complement our previous study conducted in males and contribute to the understanding of sex differences in a response to CR during aging.",
journal = "The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences",
title = "Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.",
number = "5",
volume = "77",
doi = "10.1093/gerona/glab353",
pages = "glab353-947-955"
}

Prvulović, M., Milanović, D., Vujović, P., Jović, M., Kanazir, S., Todorović, S.,& Mladenović, A.. (2021). Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.. in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 77(5), glab353-955.
https://doi.org/10.1093/gerona/glab353

Prvulović M, Milanović D, Vujović P, Jović M, Kanazir S, Todorović S, Mladenović A. Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.. in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences. 2021;77(5):glab353-955.
doi:10.1093/gerona/glab353 .

Prvulović, Milica, Milanović, Desanka, Vujović, Predrag, Jović, Milena, Kanazir, Selma, Todorović, Smilja, Mladenović, Aleksandra, "Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats." in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 77, no. 5 (2021):glab353-955,
https://doi.org/10.1093/gerona/glab353 . .

TY  - JOUR
AU  - Mladenović, Aleksandra
AU  - Kapetanou, Marianna
AU  - Lončarević Vasiljković, Nataša
AU  - Todorović, Smilja
AU  - Athanasopoulou, Sofia
AU  - Jović, Milena
AU  - Prvulović, Milica
AU  - Taoufik, Era
AU  - Matsas, Rebecca
AU  - Kanazir, Selma
AU  - Gonos, Efstathios S.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4074
AB  - Alzheimer's disease (AD) is the most common form of dementia worldwide, characterized by a progressive decline in a variety of cognitive and non-cognitive functions. The amyloid beta protein cascade hypothesis places the formation of amyloid beta protein aggregates on the first position in the complex pathological cascade leading to neurodegeneration, and therefore AD might be considered to be a protein-misfolding disease. The Ubiquitin Proteasome System (UPS), being the primary protein degradation mechanism with a fundamental role in the maintenance of proteostasis, has been identified as a putative therapeutic target to delay and/or to decelerate the progression of neurodegenerative disorders that are characterized by accumulated/aggregated proteins. The purpose of this study was to test if the activation of proteasome in vivo can alleviate AD pathology. Specifically by using two compounds with complementary modes of proteasome activation and documented antioxidant and redox regulating properties in the 5xFAD transgenic mice model of AD, we ameliorated a number of AD related deficits. Shortly after proteasome activation we detected significantly reduced amyloid-beta load correlated with improved motor functions, reduced anxiety and frailty level. Essentially, to our knowledge this is the first report to demonstrate a dual activation of the proteasome and its downstream effects. In conclusion, these findings open up new directions for future therapeutic potential of proteasome-mediated proteolysis enhancement.
PB  - Elsevier Inc.
T2  - Free Radical Biology and Medicine
T1  - Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype: Involvement of proteasome activation
VL  - 162
DO  - 10.1016/j.freeradbiomed.2020.11.038
SP  - 88
EP  - 103
ER  - 

@article{
author = "Mladenović, Aleksandra and Kapetanou, Marianna and Lončarević Vasiljković, Nataša and Todorović, Smilja and Athanasopoulou, Sofia and Jović, Milena and Prvulović, Milica and Taoufik, Era and Matsas, Rebecca and Kanazir, Selma and Gonos, Efstathios S.",
year = "2021",
abstract = "Alzheimer's disease (AD) is the most common form of dementia worldwide, characterized by a progressive decline in a variety of cognitive and non-cognitive functions. The amyloid beta protein cascade hypothesis places the formation of amyloid beta protein aggregates on the first position in the complex pathological cascade leading to neurodegeneration, and therefore AD might be considered to be a protein-misfolding disease. The Ubiquitin Proteasome System (UPS), being the primary protein degradation mechanism with a fundamental role in the maintenance of proteostasis, has been identified as a putative therapeutic target to delay and/or to decelerate the progression of neurodegenerative disorders that are characterized by accumulated/aggregated proteins. The purpose of this study was to test if the activation of proteasome in vivo can alleviate AD pathology. Specifically by using two compounds with complementary modes of proteasome activation and documented antioxidant and redox regulating properties in the 5xFAD transgenic mice model of AD, we ameliorated a number of AD related deficits. Shortly after proteasome activation we detected significantly reduced amyloid-beta load correlated with improved motor functions, reduced anxiety and frailty level. Essentially, to our knowledge this is the first report to demonstrate a dual activation of the proteasome and its downstream effects. In conclusion, these findings open up new directions for future therapeutic potential of proteasome-mediated proteolysis enhancement.",
publisher = "Elsevier Inc.",
journal = "Free Radical Biology and Medicine",
title = "Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype: Involvement of proteasome activation",
volume = "162",
doi = "10.1016/j.freeradbiomed.2020.11.038",
pages = "88-103"
}

Mladenović, A., Kapetanou, M., Lončarević Vasiljković, N., Todorović, S., Athanasopoulou, S., Jović, M., Prvulović, M., Taoufik, E., Matsas, R., Kanazir, S.,& Gonos, E. S.. (2021). Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype: Involvement of proteasome activation. in Free Radical Biology and Medicine
Elsevier Inc.., 162, 88-103.
https://doi.org/10.1016/j.freeradbiomed.2020.11.038

Mladenović A, Kapetanou M, Lončarević Vasiljković N, Todorović S, Athanasopoulou S, Jović M, Prvulović M, Taoufik E, Matsas R, Kanazir S, Gonos ES. Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype: Involvement of proteasome activation. in Free Radical Biology and Medicine. 2021;162:88-103.
doi:10.1016/j.freeradbiomed.2020.11.038 .

Mladenović, Aleksandra, Kapetanou, Marianna, Lončarević Vasiljković, Nataša, Todorović, Smilja, Athanasopoulou, Sofia, Jović, Milena, Prvulović, Milica, Taoufik, Era, Matsas, Rebecca, Kanazir, Selma, Gonos, Efstathios S., "Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype: Involvement of proteasome activation" in Free Radical Biology and Medicine, 162 (2021):88-103,
https://doi.org/10.1016/j.freeradbiomed.2020.11.038 . .

TY  - JOUR
AU  - Vesković, Ana
AU  - Nakarada, Đura
AU  - Pavićević, Aleksandra
AU  - Prokić, Bogomir
AU  - Perović, Milka
AU  - Kanazir, Selma
AU  - Popović-Bijelić, Ana
AU  - Mojović, Miloš
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4221
AB  - BACKGROUND Alzheimer's disease (AD) is the most common neurodegenerative disorder characterized by cognitive decline and total brain atrophy. Despite the substantial scientific effort, the pathological mechanisms underlying neurodegeneration in AD are currently unknown. In most studies, amyloid β peptide has been considered the key pathological change in AD. However, numerous Aβ-targeting treatments have failed in clinical trials. This implies the need to shift the re- search focus from Aβ to other pathological features of the disease. OBJECTIVE The aim of this study was to examine the interplay between mitochondrial dysfunction, oxidative stress and blood-brain barrier (BBB) disruption in AD pathology, using a novel approach that involves the application of electron paramagnetic resonance (EPR) spectroscopy. METHOD In vivo and ex vivo EPR spectroscopy using two spin probes (aminoxyl radicals) exhibit- ing different cell-membrane and BBB permeability were employed to assess BBB integrity and brain tissue redox status in the 5xFAD mouse model of AD. In vivo spin probe reduction decay was analyzed using a two-compartment pharmacokinetic model. Furthermore, 15 K EPR spectros- copy was employed to investigate the brain metal content. RESULTS This study has revealed an altered brain redox state, BBB breakdown, as well as ROS-me- diated damage to mitochondrial iron-sulfur clusters, and up-regulation of MnSOD in the 5xFAD model. CONCLUSION The EPR spin probes were shown to be excellent in vivo reporters of the 5xFAD neu- ronal tissue redox state, as well as the BBB integrity, indicating the importance of in vivo EPR spec- troscopy application in preclinical studies of neurodegenerative diseases.
PB  - Bentham Science Publishers Ltd.
T2  - Current Alzheimer Research
T1  - In vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood--Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease.
IS  - 1
VL  - 18
DO  - 10.2174/1567205018666210324121156
SP  - 25
EP  - 34
ER  - 

@article{
author = "Vesković, Ana and Nakarada, Đura and Pavićević, Aleksandra and Prokić, Bogomir and Perović, Milka and Kanazir, Selma and Popović-Bijelić, Ana and Mojović, Miloš",
year = "2021",
abstract = "BACKGROUND Alzheimer's disease (AD) is the most common neurodegenerative disorder characterized by cognitive decline and total brain atrophy. Despite the substantial scientific effort, the pathological mechanisms underlying neurodegeneration in AD are currently unknown. In most studies, amyloid β peptide has been considered the key pathological change in AD. However, numerous Aβ-targeting treatments have failed in clinical trials. This implies the need to shift the re- search focus from Aβ to other pathological features of the disease. OBJECTIVE The aim of this study was to examine the interplay between mitochondrial dysfunction, oxidative stress and blood-brain barrier (BBB) disruption in AD pathology, using a novel approach that involves the application of electron paramagnetic resonance (EPR) spectroscopy. METHOD In vivo and ex vivo EPR spectroscopy using two spin probes (aminoxyl radicals) exhibit- ing different cell-membrane and BBB permeability were employed to assess BBB integrity and brain tissue redox status in the 5xFAD mouse model of AD. In vivo spin probe reduction decay was analyzed using a two-compartment pharmacokinetic model. Furthermore, 15 K EPR spectros- copy was employed to investigate the brain metal content. RESULTS This study has revealed an altered brain redox state, BBB breakdown, as well as ROS-me- diated damage to mitochondrial iron-sulfur clusters, and up-regulation of MnSOD in the 5xFAD model. CONCLUSION The EPR spin probes were shown to be excellent in vivo reporters of the 5xFAD neu- ronal tissue redox state, as well as the BBB integrity, indicating the importance of in vivo EPR spec- troscopy application in preclinical studies of neurodegenerative diseases.",
publisher = "Bentham Science Publishers Ltd.",
journal = "Current Alzheimer Research",
title = "In vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood--Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease.",
number = "1",
volume = "18",
doi = "10.2174/1567205018666210324121156",
pages = "25-34"
}

Vesković, A., Nakarada, Đ., Pavićević, A., Prokić, B., Perović, M., Kanazir, S., Popović-Bijelić, A.,& Mojović, M.. (2021). In vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood--Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease.. in Current Alzheimer Research
Bentham Science Publishers Ltd.., 18(1), 25-34.
https://doi.org/10.2174/1567205018666210324121156

Vesković A, Nakarada Đ, Pavićević A, Prokić B, Perović M, Kanazir S, Popović-Bijelić A, Mojović M. In vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood--Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease.. in Current Alzheimer Research. 2021;18(1):25-34.
doi:10.2174/1567205018666210324121156 .

Vesković, Ana, Nakarada, Đura, Pavićević, Aleksandra, Prokić, Bogomir, Perović, Milka, Kanazir, Selma, Popović-Bijelić, Ana, Mojović, Miloš, "In vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood--Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease." in Current Alzheimer Research, 18, no. 1 (2021):25-34,
https://doi.org/10.2174/1567205018666210324121156 . .

TY  - JOUR
AU  - Tešić, Vesna
AU  - Ćirić, Jelena
AU  - Jovanović Macura, Irena
AU  - Zogović, Nevena
AU  - Milanović, Desanka
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2021
UR  - https://www.mdpi.com/2072-6643/13/12/4526
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8703853
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4760
AB  - Numerous beneficial effects of food restriction on aging and age-related pathologies are well documented. It is also well-established that both short- and long-term food restriction regimens induce elevated circulating levels of glucocorticoids, stress-induced hormones produced by adrenal glands that can also exert deleterious effects on the brain. In the present study, we examined the effect of long-term food restriction on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the cortex during aging, in 18- and 24-month-old rats. Corticosterone level was increased in the cortex of aged ad libitum-fed rats. Food restriction induced its further increase, accompanied with an increase in the level of 11β-hydroxysteroid dehydrogenase type 1. However, alterations in the level of GR phosphorylated at Ser232 were not detected in animals on food restriction, in line with unaltered CDK5 level, the decrease of Hsp90, and an increase in a negative regulator of GR function, FKBP51. Moreover, our data revealed that reduced food intake prevented age-related increase in the levels of NFκB, gfap, and bax, confirming its anti-inflammatory and anti-apoptotic effects. Along with an increase in the levels of c-fos, our study provides additional evidences that food restriction affects cortical responsiveness to glucocorticoids during aging.
PB  - Basel: MDPI
T2  - Nutrients
T1  - Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.
IS  - 12
VL  - 13
DO  - 10.3390/nu13124526
SP  - 4526
ER  - 

@article{
author = "Tešić, Vesna and Ćirić, Jelena and Jovanović Macura, Irena and Zogović, Nevena and Milanović, Desanka and Kanazir, Selma and Perović, Milka",
year = "2021",
abstract = "Numerous beneficial effects of food restriction on aging and age-related pathologies are well documented. It is also well-established that both short- and long-term food restriction regimens induce elevated circulating levels of glucocorticoids, stress-induced hormones produced by adrenal glands that can also exert deleterious effects on the brain. In the present study, we examined the effect of long-term food restriction on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the cortex during aging, in 18- and 24-month-old rats. Corticosterone level was increased in the cortex of aged ad libitum-fed rats. Food restriction induced its further increase, accompanied with an increase in the level of 11β-hydroxysteroid dehydrogenase type 1. However, alterations in the level of GR phosphorylated at Ser232 were not detected in animals on food restriction, in line with unaltered CDK5 level, the decrease of Hsp90, and an increase in a negative regulator of GR function, FKBP51. Moreover, our data revealed that reduced food intake prevented age-related increase in the levels of NFκB, gfap, and bax, confirming its anti-inflammatory and anti-apoptotic effects. Along with an increase in the levels of c-fos, our study provides additional evidences that food restriction affects cortical responsiveness to glucocorticoids during aging.",
publisher = "Basel: MDPI",
journal = "Nutrients",
title = "Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.",
number = "12",
volume = "13",
doi = "10.3390/nu13124526",
pages = "4526"
}

Tešić, V., Ćirić, J., Jovanović Macura, I., Zogović, N., Milanović, D., Kanazir, S.,& Perović, M.. (2021). Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.. in Nutrients
Basel: MDPI., 13(12), 4526.
https://doi.org/10.3390/nu13124526

Tešić V, Ćirić J, Jovanović Macura I, Zogović N, Milanović D, Kanazir S, Perović M. Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.. in Nutrients. 2021;13(12):4526.
doi:10.3390/nu13124526 .

Tešić, Vesna, Ćirić, Jelena, Jovanović Macura, Irena, Zogović, Nevena, Milanović, Desanka, Kanazir, Selma, Perović, Milka, "Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction." in Nutrients, 13, no. 12 (2021):4526,
https://doi.org/10.3390/nu13124526 . .

TY  - JOUR
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Lončarević-Vasiljković, Nataša
AU  - Kanazir, Selma
AU  - Pešić, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4073
AB  - Reduction in direct social contact with peers during early adolescence is thought to be a risk factor for an increase in depressive symptoms, but there is still no clear evidence to suggest early behavioral manifestations and their association with the later outcome of social distancing during this period. To address this question, we used social isolation paradigm in peripubertal rats as the rodent model of adolescence. The litter was an experimental unit. On postnatal day 29, each litter gave group-housed and single-housed males, which were reared and tested one week and two weeks thereafter. Psychomotor/emotional response to novelty in exploration-based tasks, behavioral and neuronal responses to the drug reward (D-amphetamine), motivation/hedonic behavior, physiological and response to physiological stress were examined. Social isolation in peripubertal rats manifested through: hyper-reactivity/agitation and the state anxiety/risk-taking at an early stage; reduced behavioral response to D-amphetamine and altered neural processing of this stimulus, at a later stage; consummatory hypohedonia that deepened over time without changing the motivation to eat; unchanged body weight gain and resting blood corticosterone, cortisol and glucose levels over time; altered blood biochemistry (silenced corticosterone and increased glucose) due to overnight fasting only at an early stage. Our results highlight that the outcome of reduced direct social contact with peers during peripuberty is dynamic, with the cluster of atypical early symptoms that evolve into the syndrome that is delicate for assessment through routinely measurable behavior and biomarkers of stress, but with progressive consummatory hypohedonia and unaffected motivation to eat as stable marks.
PB  - Elsevier Inc.
T2  - Progress in Neuro-Psychopharmacology and Biological Psychiatry
T1  - Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty
VL  - 108
DO  - 10.1016/j.pnpbp.2020.110186
SP  - 110186
ER  - 

@article{
author = "Potrebić, Milica and Pavković, Željko and Lončarević-Vasiljković, Nataša and Kanazir, Selma and Pešić, Vesna",
year = "2021",
abstract = "Reduction in direct social contact with peers during early adolescence is thought to be a risk factor for an increase in depressive symptoms, but there is still no clear evidence to suggest early behavioral manifestations and their association with the later outcome of social distancing during this period. To address this question, we used social isolation paradigm in peripubertal rats as the rodent model of adolescence. The litter was an experimental unit. On postnatal day 29, each litter gave group-housed and single-housed males, which were reared and tested one week and two weeks thereafter. Psychomotor/emotional response to novelty in exploration-based tasks, behavioral and neuronal responses to the drug reward (D-amphetamine), motivation/hedonic behavior, physiological and response to physiological stress were examined. Social isolation in peripubertal rats manifested through: hyper-reactivity/agitation and the state anxiety/risk-taking at an early stage; reduced behavioral response to D-amphetamine and altered neural processing of this stimulus, at a later stage; consummatory hypohedonia that deepened over time without changing the motivation to eat; unchanged body weight gain and resting blood corticosterone, cortisol and glucose levels over time; altered blood biochemistry (silenced corticosterone and increased glucose) due to overnight fasting only at an early stage. Our results highlight that the outcome of reduced direct social contact with peers during peripuberty is dynamic, with the cluster of atypical early symptoms that evolve into the syndrome that is delicate for assessment through routinely measurable behavior and biomarkers of stress, but with progressive consummatory hypohedonia and unaffected motivation to eat as stable marks.",
publisher = "Elsevier Inc.",
journal = "Progress in Neuro-Psychopharmacology and Biological Psychiatry",
title = "Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty",
volume = "108",
doi = "10.1016/j.pnpbp.2020.110186",
pages = "110186"
}

Potrebić, M., Pavković, Ž., Lončarević-Vasiljković, N., Kanazir, S.,& Pešić, V.. (2021). Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty. in Progress in Neuro-Psychopharmacology and Biological Psychiatry
Elsevier Inc.., 108, 110186.
https://doi.org/10.1016/j.pnpbp.2020.110186

Potrebić M, Pavković Ž, Lončarević-Vasiljković N, Kanazir S, Pešić V. Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty. in Progress in Neuro-Psychopharmacology and Biological Psychiatry. 2021;108:110186.
doi:10.1016/j.pnpbp.2020.110186 .

Potrebić, Milica, Pavković, Željko, Lončarević-Vasiljković, Nataša, Kanazir, Selma, Pešić, Vesna, "Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty" in Progress in Neuro-Psychopharmacology and Biological Psychiatry, 108 (2021):110186,
https://doi.org/10.1016/j.pnpbp.2020.110186 . .

TY  - CONF
AU  - Prvulović, Milica
AU  - Todorović, Smilja
AU  - Kanazir, Selma
AU  - Mladenović, Aleksandra
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4804
AB  - The continuous increase of a lifespan imposes need to prolong the healthspan - period of life spent free of age-related disabilities. Some interventions such as dietary restriction (DR) can retard aging process, improve quality of life and increase healthspan. However, recent studies have re-evaluated generality of DR’s beneficial effect, as different outcomes of DR have been reported in dependence of onset and duration of DR. In order to test the impact of various dietary restricted regimens in female rats during aging, we used frailty assessment as one of the best indicators of healthspan. Female Wistar rats of various age (young adult, middle-age and aged) were exposed to DR (60 % of AL daily intake) feeding regimens. We determined and compared frailty index (FI) during aging in ad libitum fed (AL) and in rats exposed to DR. Frailty index was constructed using 22 parameters. FI was increased in both 18- and 24-month-old AL animals in comparison to young control counterparts. Life-long type of restricted diet decreased FI in 18- month old group while shorter duration of DR regimens failed to lower frailty index in 18- and 24- month-old animals. Our results indicated that life-long DR can alter animal’s frailty during aging, while shorter duration and later onset DR have no effect on FI in female rats
PB  - Elsevier Inc.
C3  - Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
T1  - Frailty index of aging female rats changes under diferent dietary protocols
DO  - 10.1016/j.freeradbiomed.2021.08.134
SP  - S96
ER  - 

@conference{
author = "Prvulović, Milica and Todorović, Smilja and Kanazir, Selma and Mladenović, Aleksandra",
year = "2021",
abstract = "The continuous increase of a lifespan imposes need to prolong the healthspan - period of life spent free of age-related disabilities. Some interventions such as dietary restriction (DR) can retard aging process, improve quality of life and increase healthspan. However, recent studies have re-evaluated generality of DR’s beneficial effect, as different outcomes of DR have been reported in dependence of onset and duration of DR. In order to test the impact of various dietary restricted regimens in female rats during aging, we used frailty assessment as one of the best indicators of healthspan. Female Wistar rats of various age (young adult, middle-age and aged) were exposed to DR (60 % of AL daily intake) feeding regimens. We determined and compared frailty index (FI) during aging in ad libitum fed (AL) and in rats exposed to DR. Frailty index was constructed using 22 parameters. FI was increased in both 18- and 24-month-old AL animals in comparison to young control counterparts. Life-long type of restricted diet decreased FI in 18- month old group while shorter duration of DR regimens failed to lower frailty index in 18- and 24- month-old animals. Our results indicated that life-long DR can alter animal’s frailty during aging, while shorter duration and later onset DR have no effect on FI in female rats",
publisher = "Elsevier Inc.",
journal = "Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia",
title = "Frailty index of aging female rats changes under diferent dietary protocols",
doi = "10.1016/j.freeradbiomed.2021.08.134",
pages = "S96"
}

Prvulović, M., Todorović, S., Kanazir, S.,& Mladenović, A.. (2021). Frailty index of aging female rats changes under diferent dietary protocols. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
Elsevier Inc.., S96.
https://doi.org/10.1016/j.freeradbiomed.2021.08.134

Prvulović M, Todorović S, Kanazir S, Mladenović A. Frailty index of aging female rats changes under diferent dietary protocols. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia. 2021;:S96.
doi:10.1016/j.freeradbiomed.2021.08.134 .

Prvulović, Milica, Todorović, Smilja, Kanazir, Selma, Mladenović, Aleksandra, "Frailty index of aging female rats changes under diferent dietary protocols" in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia (2021):S96,
https://doi.org/10.1016/j.freeradbiomed.2021.08.134 . .

TY  - GEN
AU  - Kanazir, Selma
AU  - Mladenović, Aleksandra
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4151
PB  - Elsevier Ireland Ltd.
T2  - Mechanisms of Ageing and Development
T1  - Editorial
VL  - 195
DO  - 10.1016/j.mad.2021.111440
SP  - 111440
ER  - 

@misc{
author = "Kanazir, Selma and Mladenović, Aleksandra",
year = "2021",
publisher = "Elsevier Ireland Ltd.",
journal = "Mechanisms of Ageing and Development",
title = "Editorial",
volume = "195",
doi = "10.1016/j.mad.2021.111440",
pages = "111440"
}

Kanazir, S.,& Mladenović, A.. (2021). Editorial. in Mechanisms of Ageing and Development
Elsevier Ireland Ltd.., 195, 111440.
https://doi.org/10.1016/j.mad.2021.111440

Kanazir S, Mladenović A. Editorial. in Mechanisms of Ageing and Development. 2021;195:111440.
doi:10.1016/j.mad.2021.111440 .

Kanazir, Selma, Mladenović, Aleksandra, "Editorial" in Mechanisms of Ageing and Development, 195 (2021):111440,
https://doi.org/10.1016/j.mad.2021.111440 . .

TY  - CONF
AU  - Prvulović, Milica
AU  - Todorović, Smilja
AU  - Jović, Milena
AU  - Lončarević-Vasiljković, Nataša
AU  - Kanazir, Selma
AU  - Mladenović, Aleksandra
PY  - 2020
UR  - https://forum2020.fens.org/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5824
AB  - Objectives: Increased frailty is associated with the progression of Alzheimer’s disease (AD), characterized by the accumulation of amyloid plaques composed of aggregated amyloid-beta (Abeta) peptides, mainly Abeta 42. It has been shown that substances like 18 alpha- glycyrrhetinic acid (18-alpha GA) are able to enhance activity of ubiquitin-proteasome system responsible for the removal of proteins, and to decrease Abeta deposits in model organisms. We examined the effect of 18-alpha GA supplementation on APP processing and generation of Abeta 42 in the 5xFAD transgenic AD mouse model, characterized by the deposition of plaques in the cortex and hippocampus as early as at 2 months of age.
Methods: Both female and male mice were exposed to the 18-alpha GA treatment for six months, starting from 2-months of age which is considered an early phase of AD pathology, suitable for therapeutics application. Frailty was determined in control young (2 months) and old (12-13 months of age) mice, as well in those treated with 18-alpha-GA, by using phenotype frailty score (FS) and clinical frailty index (FI).  
Results: Obtained results showed a significant increase in frailty in aged 5xFAD mice. Significant differences were observed between males and females. 18-alpha-GA treatment increased activity of the proteasome in the cortex and hippocampus of 5xFAD mice, decreased the number of Abeta plaques and decreased frailty level.
Conclusions: Results indicate that 18-alpha-GA has a therapeutic potential in treatment of AD pathology.
PB  - Federation of European Neuroscience Societies
C3  - 12 FENS Forum of Neuroscience; 2020 Jul 11-15; Virtual Forum
T1  - Frailty in 5xFAD mouse model of Alzheimer’s disease: The influence of age and proteasome activation
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5824
ER  - 

@conference{
author = "Prvulović, Milica and Todorović, Smilja and Jović, Milena and Lončarević-Vasiljković, Nataša and Kanazir, Selma and Mladenović, Aleksandra",
year = "2020",
abstract = "Objectives: Increased frailty is associated with the progression of Alzheimer’s disease (AD), characterized by the accumulation of amyloid plaques composed of aggregated amyloid-beta (Abeta) peptides, mainly Abeta 42. It has been shown that substances like 18 alpha- glycyrrhetinic acid (18-alpha GA) are able to enhance activity of ubiquitin-proteasome system responsible for the removal of proteins, and to decrease Abeta deposits in model organisms. We examined the effect of 18-alpha GA supplementation on APP processing and generation of Abeta 42 in the 5xFAD transgenic AD mouse model, characterized by the deposition of plaques in the cortex and hippocampus as early as at 2 months of age.
Methods: Both female and male mice were exposed to the 18-alpha GA treatment for six months, starting from 2-months of age which is considered an early phase of AD pathology, suitable for therapeutics application. Frailty was determined in control young (2 months) and old (12-13 months of age) mice, as well in those treated with 18-alpha-GA, by using phenotype frailty score (FS) and clinical frailty index (FI).  
Results: Obtained results showed a significant increase in frailty in aged 5xFAD mice. Significant differences were observed between males and females. 18-alpha-GA treatment increased activity of the proteasome in the cortex and hippocampus of 5xFAD mice, decreased the number of Abeta plaques and decreased frailty level.
Conclusions: Results indicate that 18-alpha-GA has a therapeutic potential in treatment of AD pathology.",
publisher = "Federation of European Neuroscience Societies",
journal = "12 FENS Forum of Neuroscience; 2020 Jul 11-15; Virtual Forum",
title = "Frailty in 5xFAD mouse model of Alzheimer’s disease: The influence of age and proteasome activation",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5824"
}

Prvulović, M., Todorović, S., Jović, M., Lončarević-Vasiljković, N., Kanazir, S.,& Mladenović, A.. (2020). Frailty in 5xFAD mouse model of Alzheimer’s disease: The influence of age and proteasome activation. in 12 FENS Forum of Neuroscience; 2020 Jul 11-15; Virtual Forum
Federation of European Neuroscience Societies..
https://hdl.handle.net/21.15107/rcub_ibiss_5824

Prvulović M, Todorović S, Jović M, Lončarević-Vasiljković N, Kanazir S, Mladenović A. Frailty in 5xFAD mouse model of Alzheimer’s disease: The influence of age and proteasome activation. in 12 FENS Forum of Neuroscience; 2020 Jul 11-15; Virtual Forum. 2020;.
https://hdl.handle.net/21.15107/rcub_ibiss_5824 .

Prvulović, Milica, Todorović, Smilja, Jović, Milena, Lončarević-Vasiljković, Nataša, Kanazir, Selma, Mladenović, Aleksandra, "Frailty in 5xFAD mouse model of Alzheimer’s disease: The influence of age and proteasome activation" in 12 FENS Forum of Neuroscience; 2020 Jul 11-15; Virtual Forum (2020),
https://hdl.handle.net/21.15107/rcub_ibiss_5824 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Kanazir, Selma
PY  - 2020
UR  - http://hrana-ishrana.org/wp-content/uploads/2021/03/Broj-2-Vol-61-2020-1.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4180
AB  - Sve veći broj podataka ukazuje na to da infekcija virusom SARS-CoV-2
može uticati na nervni sistem. Ovde predstavljamo kratak, uzdržan pregled
opisanih neuroloških oštećenja povezanih sa SARS-CoV-2 infekcijom. Iako
je očigledno da se neurološki poremećaji mogu dijagnostikovati kod dela
COVID-19 pacijenata, čvrsti dokazi o neurovirulenciji SARS-CoV-2 još uvek
nedostaju. Postojeći podaci o učestalosti neuroloških poremećaja među
COVID-19 pacijentima veoma su raznoliki, verovatno zato što (najčešće)
potiču iz malih, unicentričnih retrospektivnih studija. Ovi podaci se praktično
objavljuju u realnom vremenu i ostaje pitanje kada će biti dostupne veće
studije, s obzirom na to da se pandemija nastavlja. Ovde se ukratko bavimo
i drugim pitanjima u vezi sa neurološkim poremećajima i pandemijom COVID-
19, uključujući tu i uticaj pandemije na ljude sa postojećim hroničnim
neurološkim poremećajima i moguće dugoročne neurološke posledice infekcije
SARS-CoV-2.
AB  - The growing body of data implies that SARS-CoV-2 infection may affect
the nervous system. We here present a short, taciturn overview of
described neurological impairments related to SARS-CoV-2 infection.
While it is obvious that neurological impairments can be diagnosed in a
portion of COVID-19 patients, evidence of SARS-CoV-2 neurovirulence in
humans is still lacking. The existing data on the incidence of neurological
impairments among COVID-19 patients is highly variable, probably
because they (most often) come from small, single-center retrospective
studies. These data are practically published in real-time, and the
question remains when larger studies will be available, given that the
pandemic is continuing. We here also shortly address the other issues
related to neurological disorders and COVID-19 pandemic, including
the concern for people with existing chronic neurological disorders and
possible long-term neurological consequences of SARS-CoV-2 infection.
PB  - Belgrade: Serbian Nutrition Society
T2  - Hrana i ishrana
T1  - Neurological impairments in COVID-19 pandemic
T1  - Neurološki poremećaji i pandemija Covid -19
IS  - 2
VL  - 61
DO  - 10.5937/hraIsh2002071B
SP  - 71
EP  - 77
ER  - 

@article{
author = "Bjelobaba, Ivana and Kanazir, Selma",
year = "2020",
abstract = "Sve veći broj podataka ukazuje na to da infekcija virusom SARS-CoV-2
može uticati na nervni sistem. Ovde predstavljamo kratak, uzdržan pregled
opisanih neuroloških oštećenja povezanih sa SARS-CoV-2 infekcijom. Iako
je očigledno da se neurološki poremećaji mogu dijagnostikovati kod dela
COVID-19 pacijenata, čvrsti dokazi o neurovirulenciji SARS-CoV-2 još uvek
nedostaju. Postojeći podaci o učestalosti neuroloških poremećaja među
COVID-19 pacijentima veoma su raznoliki, verovatno zato što (najčešće)
potiču iz malih, unicentričnih retrospektivnih studija. Ovi podaci se praktično
objavljuju u realnom vremenu i ostaje pitanje kada će biti dostupne veće
studije, s obzirom na to da se pandemija nastavlja. Ovde se ukratko bavimo
i drugim pitanjima u vezi sa neurološkim poremećajima i pandemijom COVID-
19, uključujući tu i uticaj pandemije na ljude sa postojećim hroničnim
neurološkim poremećajima i moguće dugoročne neurološke posledice infekcije
SARS-CoV-2., The growing body of data implies that SARS-CoV-2 infection may affect
the nervous system. We here present a short, taciturn overview of
described neurological impairments related to SARS-CoV-2 infection.
While it is obvious that neurological impairments can be diagnosed in a
portion of COVID-19 patients, evidence of SARS-CoV-2 neurovirulence in
humans is still lacking. The existing data on the incidence of neurological
impairments among COVID-19 patients is highly variable, probably
because they (most often) come from small, single-center retrospective
studies. These data are practically published in real-time, and the
question remains when larger studies will be available, given that the
pandemic is continuing. We here also shortly address the other issues
related to neurological disorders and COVID-19 pandemic, including
the concern for people with existing chronic neurological disorders and
possible long-term neurological consequences of SARS-CoV-2 infection.",
publisher = "Belgrade: Serbian Nutrition Society",
journal = "Hrana i ishrana",
title = "Neurological impairments in COVID-19 pandemic, Neurološki poremećaji i pandemija Covid -19",
number = "2",
volume = "61",
doi = "10.5937/hraIsh2002071B",
pages = "71-77"
}

Bjelobaba, I.,& Kanazir, S.. (2020). Neurological impairments in COVID-19 pandemic. in Hrana i ishrana
Belgrade: Serbian Nutrition Society., 61(2), 71-77.
https://doi.org/10.5937/hraIsh2002071B

Bjelobaba I, Kanazir S. Neurological impairments in COVID-19 pandemic. in Hrana i ishrana. 2020;61(2):71-77.
doi:10.5937/hraIsh2002071B .

Bjelobaba, Ivana, Kanazir, Selma, "Neurological impairments in COVID-19 pandemic" in Hrana i ishrana, 61, no. 2 (2020):71-77,
https://doi.org/10.5937/hraIsh2002071B . .