TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Jevremović, Slađana
AU  - Uzelac, Branka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6522
AB  - The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime
IS  - 1
VL  - 10
DO  - 10.3390/horticulturae10010017
SP  - 17
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Jevremović, Slađana and Uzelac, Branka",
year = "2024",
abstract = "The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime",
number = "1",
volume = "10",
doi = "10.3390/horticulturae10010017",
pages = "17"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Jevremović, S.,& Uzelac, B.. (2024). Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae
Basel : MDPI., 10(1), 17.
https://doi.org/10.3390/horticulturae10010017

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Jevremović S, Uzelac B. Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae. 2024;10(1):17.
doi:10.3390/horticulturae10010017 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Jevremović, Slađana, Uzelac, Branka, "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime" in Horticulturae, 10, no. 1 (2024):17,
https://doi.org/10.3390/horticulturae10010017 . .

TY  - JOUR
AU  - Popržen, Tatjana
AU  - Nikolić, Ivan
AU  - Krstić-Milošević, Dijana
AU  - Uzelac, Branka
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
AU  - Radulović, Olga
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6442
AB  - The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).
PB  - Basel : MDPI
T2  - International Journal of Molecular Sciences
T1  - Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)
IS  - 24
VL  - 24
DO  - 10.3390/ijms242417207
SP  - 17207
ER  - 

@article{
author = "Popržen, Tatjana and Nikolić, Ivan and Krstić-Milošević, Dijana and Uzelac, Branka and Trifunović-Momčilov, Milana and Marković, Marija and Radulović, Olga",
year = "2023",
abstract = "The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).",
publisher = "Basel : MDPI",
journal = "International Journal of Molecular Sciences",
title = "Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)",
number = "24",
volume = "24",
doi = "10.3390/ijms242417207",
pages = "17207"
}

Popržen, T., Nikolić, I., Krstić-Milošević, D., Uzelac, B., Trifunović-Momčilov, M., Marković, M.,& Radulović, O.. (2023). Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.). in International Journal of Molecular Sciences
Basel : MDPI., 24(24), 17207.
https://doi.org/10.3390/ijms242417207

Popržen T, Nikolić I, Krstić-Milošević D, Uzelac B, Trifunović-Momčilov M, Marković M, Radulović O. Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.). in International Journal of Molecular Sciences. 2023;24(24):17207.
doi:10.3390/ijms242417207 .

Popržen, Tatjana, Nikolić, Ivan, Krstić-Milošević, Dijana, Uzelac, Branka, Trifunović-Momčilov, Milana, Marković, Marija, Radulović, Olga, "Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)" in International Journal of Molecular Sciences, 24, no. 24 (2023):17207,
https://doi.org/10.3390/ijms242417207 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Radulović, Olga
AU  - Subotić, Angelina
AU  - Uzelac, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6135
AB  - Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H2O2, total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H2O2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana. The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.
PB  - Basel: MDPI
T2  - Agriculture
T1  - Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?
IS  - 10
VL  - 13
DO  - 10.3390/agriculture13101903
SP  - 1903
ER  - 

@article{
author = "Đurić, Marija and Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Radulović, Olga and Subotić, Angelina and Uzelac, Branka",
year = "2023",
abstract = "Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H2O2, total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H2O2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana. The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.",
publisher = "Basel: MDPI",
journal = "Agriculture",
title = "Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?",
number = "10",
volume = "13",
doi = "10.3390/agriculture13101903",
pages = "1903"
}

Đurić, M., Trifunović-Momčilov, M., Milošević, S., Marković, M., Radulović, O., Subotić, A.,& Uzelac, B.. (2023). Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?. in Agriculture
Basel: MDPI., 13(10), 1903.
https://doi.org/10.3390/agriculture13101903

Đurić M, Trifunović-Momčilov M, Milošević S, Marković M, Radulović O, Subotić A, Uzelac B. Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?. in Agriculture. 2023;13(10):1903.
doi:10.3390/agriculture13101903 .

Đurić, Marija, Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Radulović, Olga, Subotić, Angelina, Uzelac, Branka, "Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?" in Agriculture, 13, no. 10 (2023):1903,
https://doi.org/10.3390/agriculture13101903 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Uzelac, Branka
AU  - Subotić, Angelina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6089
AB  - Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro
IS  - 8
VL  - 9
DO  - 10.3390/horticulturae9080910
SP  - 910
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Uzelac, Branka and Subotić, Angelina",
year = "2023",
abstract = "Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro",
number = "8",
volume = "9",
doi = "10.3390/horticulturae9080910",
pages = "910"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Uzelac, B.,& Subotić, A.. (2023). The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro. in Horticulturae
Basel : MDPI., 9(8), 910.
https://doi.org/10.3390/horticulturae9080910

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Uzelac B, Subotić A. The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro. in Horticulturae. 2023;9(8):910.
doi:10.3390/horticulturae9080910 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Uzelac, Branka, Subotić, Angelina, "The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro" in Horticulturae, 9, no. 8 (2023):910,
https://doi.org/10.3390/horticulturae9080910 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Đurić, Marija
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5335
AB  - Soil salinity is one of the most common abiotic stressors that affects plant growth and
development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP),
a donor of nitric oxide (NO), on the physiological response of common centaury (Centaurium erythraea)
shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury
shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and
250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient
media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results
showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-
2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide
dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt
stress. However, MDA concentration was decreased, while H2O2 and proline content did not drastically
change under the stress conditions caused by NaCl. Exogenous application of SNP altered
the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic
pigment content, total phenolics and proline content were noted, with reduced MDA,
but not H2O2, concentration was observed. In addition, the exogenous application of SNP increased
the degree of DPPH reduction as well as SOD, CAT and POX activities.
PB  - Basel: MDPI
T2  - Life
T1  - Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro
IS  - 1
VL  - 13
DO  - 10.3390/life13010154
SP  - 154
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Stamenković, Nikola and Đurić, Marija and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2023",
abstract = "Soil salinity is one of the most common abiotic stressors that affects plant growth and
development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP),
a donor of nitric oxide (NO), on the physiological response of common centaury (Centaurium erythraea)
shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury
shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and
250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient
media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results
showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-
2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide
dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt
stress. However, MDA concentration was decreased, while H2O2 and proline content did not drastically
change under the stress conditions caused by NaCl. Exogenous application of SNP altered
the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic
pigment content, total phenolics and proline content were noted, with reduced MDA,
but not H2O2, concentration was observed. In addition, the exogenous application of SNP increased
the degree of DPPH reduction as well as SOD, CAT and POX activities.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro",
number = "1",
volume = "13",
doi = "10.3390/life13010154",
pages = "154"
}

Trifunović-Momčilov, M., Stamenković, N., Đurić, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2023). Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro. in Life
Basel: MDPI., 13(1), 154.
https://doi.org/10.3390/life13010154

Trifunović-Momčilov M, Stamenković N, Đurić M, Milošević S, Marković M, Giba Z, Subotić A. Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro. in Life. 2023;13(1):154.
doi:10.3390/life13010154 .

Trifunović-Momčilov, Milana, Stamenković, Nikola, Đurić, Marija, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro" in Life, 13, no. 1 (2023):154,
https://doi.org/10.3390/life13010154 . .

TY  - CONF
AU  - Radulović, Olga
AU  - Popržen, Tatjana
AU  - Marković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6193
AB  - Cilj ovog rada je bila analiza rizosfere sočivice kao mesta aktivne
produkcije auksina bakterijskog porekla, primarno indol-3-acetatne kiseline (IAA). Produkcija IAA je testirana na 21 različitom bakterijskom soju. Četiri bakterijska soja: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 i Pseudomonas yamanorum 47 odabrana su na osnovu povećane produkcije IAA u odnosu na ostale testirane sojeve. Ovi sojevi su pokazali neprekinutu produkciju IAA i pod nepovoljnim uslovima: u kiseloj i baznoj sredini, kao i u hipersalinoj sredini. Naši rezultati sugerišu da ovi sojevi mogu imati biotehnološku primenu u zaštiti biljaka i povećanju prinosa putem produkcije auksina kao centralnih regulatora rasta i razvića biljaka.
AB  - The aim of this study was to analyze the rhizosphere of duckweed as a site of
active production of auxins of bacterial origin, primarily indole-3-acetic acid (IAA). IAA production was tested on 21 different bacterial strains. Four bacterial strains: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 and Pseudomonas yamanorum 47 were selected based on increased IAA production compared to other strains tested. These strains showed continuous production of IAA even under unfavorable conditions: in acidic and basic environments, as well as in hypersaline environments. Our results suggest that these strains may have biotechnological applications in plant protection and increase yields through auxin production as central regulators of plant growth and development.
PB  - Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu
C3  - Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
T1  - Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla
T1  - Potential of the rhizosphere of Duckweed (Lemna minor L.) for the production of auxins of bacterial origin
DO  - 10.46793/SBT27.401R
SP  - 401
EP  - 406
ER  - 

@conference{
author = "Radulović, Olga and Popržen, Tatjana and Marković, Marija",
year = "2022",
abstract = "Cilj ovog rada je bila analiza rizosfere sočivice kao mesta aktivne
produkcije auksina bakterijskog porekla, primarno indol-3-acetatne kiseline (IAA). Produkcija IAA je testirana na 21 različitom bakterijskom soju. Četiri bakterijska soja: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 i Pseudomonas yamanorum 47 odabrana su na osnovu povećane produkcije IAA u odnosu na ostale testirane sojeve. Ovi sojevi su pokazali neprekinutu produkciju IAA i pod nepovoljnim uslovima: u kiseloj i baznoj sredini, kao i u hipersalinoj sredini. Naši rezultati sugerišu da ovi sojevi mogu imati biotehnološku primenu u zaštiti biljaka i povećanju prinosa putem produkcije auksina kao centralnih regulatora rasta i razvića biljaka., The aim of this study was to analyze the rhizosphere of duckweed as a site of
active production of auxins of bacterial origin, primarily indole-3-acetic acid (IAA). IAA production was tested on 21 different bacterial strains. Four bacterial strains: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 and Pseudomonas yamanorum 47 were selected based on increased IAA production compared to other strains tested. These strains showed continuous production of IAA even under unfavorable conditions: in acidic and basic environments, as well as in hypersaline environments. Our results suggest that these strains may have biotechnological applications in plant protection and increase yields through auxin production as central regulators of plant growth and development.",
publisher = "Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu",
journal = "Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia",
title = "Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla, Potential of the rhizosphere of Duckweed (Lemna minor L.) for the production of auxins of bacterial origin",
doi = "10.46793/SBT27.401R",
pages = "401-406"
}

Radulović, O., Popržen, T.,& Marković, M.. (2022). Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu., 401-406.
https://doi.org/10.46793/SBT27.401R

Radulović O, Popržen T, Marković M. Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia. 2022;:401-406.
doi:10.46793/SBT27.401R .

Radulović, Olga, Popržen, Tatjana, Marković, Marija, "Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla" in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia (2022):401-406,
https://doi.org/10.46793/SBT27.401R . .

TY  - CONF
AU  - Radulović, Olga
AU  - Popržen, Tatjana
AU  - Marković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6195
AB  - Modern agriculture relies heavily on the use of synthetic herbicides: however, although efficient, these chemicals are also harmful to the environment. Therefore, there is an ever-increasing need for alternative herbicides: ethanol and indole-3-acetic acid (IAA) are among the candidates. Ethanol can be cheaply produced from a vast variety of substrates in large-scale fermentation processes. Moreover, in the last 2 decades, its applicability as an alternative, biosustainable herbicide is also being recognized. Ethanol has a well-known phytotoxic effect, although its mechanisms of actions are still poorly understood. IAA, a plant phytohormone, exerts its negative effect when applied in higher doses. Each plant has different sensitivity to ethanol and IAA. This property can be used to suppress the growth of harmful species (e.g. weeds) while stimulating the growth of desired cultures. In this study, we tested the effects of IAA and ethanol on duckweeds (Lemna minor L.). We measured non-enzymatic parameters of oxidative stress and multiplication rates of duckweeds exposed to IAA and to ethanol, respectively. Our results showed that higher concen-trations of IAA (>1 mg l-1) suppress the growth of duckweeds while even small amounts of ethanol (0.5% and 1% v/v) were sufficient to reduce the growth and biomass production of duckweeds. In conclusion, ethanol and IAA can exert significant negative effects on plant growth, at least in part by increasing oxidative stress in plants, and can therefore be potentially used as low-cost, eco-friendly herbicides.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth
SP  - 100
EP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6195
ER  - 

@conference{
author = "Radulović, Olga and Popržen, Tatjana and Marković, Marija",
year = "2022",
abstract = "Modern agriculture relies heavily on the use of synthetic herbicides: however, although efficient, these chemicals are also harmful to the environment. Therefore, there is an ever-increasing need for alternative herbicides: ethanol and indole-3-acetic acid (IAA) are among the candidates. Ethanol can be cheaply produced from a vast variety of substrates in large-scale fermentation processes. Moreover, in the last 2 decades, its applicability as an alternative, biosustainable herbicide is also being recognized. Ethanol has a well-known phytotoxic effect, although its mechanisms of actions are still poorly understood. IAA, a plant phytohormone, exerts its negative effect when applied in higher doses. Each plant has different sensitivity to ethanol and IAA. This property can be used to suppress the growth of harmful species (e.g. weeds) while stimulating the growth of desired cultures. In this study, we tested the effects of IAA and ethanol on duckweeds (Lemna minor L.). We measured non-enzymatic parameters of oxidative stress and multiplication rates of duckweeds exposed to IAA and to ethanol, respectively. Our results showed that higher concen-trations of IAA (>1 mg l-1) suppress the growth of duckweeds while even small amounts of ethanol (0.5% and 1% v/v) were sufficient to reduce the growth and biomass production of duckweeds. In conclusion, ethanol and IAA can exert significant negative effects on plant growth, at least in part by increasing oxidative stress in plants, and can therefore be potentially used as low-cost, eco-friendly herbicides.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth",
pages = "100-100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6195"
}

Radulović, O., Popržen, T.,& Marković, M.. (2022). Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 100-100.
https://hdl.handle.net/21.15107/rcub_ibiss_6195

Radulović O, Popržen T, Marković M. Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:100-100.
https://hdl.handle.net/21.15107/rcub_ibiss_6195 .

Radulović, Olga, Popržen, Tatjana, Marković, Marija, "Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):100-100,
https://hdl.handle.net/21.15107/rcub_ibiss_6195 .

TY  - CONF
AU  - Đurić, Marija
AU  - Stamenković, Nikola
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5327
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant used in traditional medicine
since ancient times. Centaury inhabits dry pastures, roadsides and fields from lowlands to
mountain vegetation. Considering that centaury can also be found on moderately saline habitats
in nature, it was interesting to investigate the effect of exogenously applied sodium nitroprusside
(SNP), as a donor of nitrogen monoxide (NO), on antioxidative enzymes activity of centaury shoots
grown under stress caused by sodium chloride (NaCl) in vitro. Centaury shoots were grown on
½MS nutrient media containing graded concentrations of SNP (0, 50, 100 and 250 μM). After three
weeks of pretreatment, shoots were further transferred to ½MS nutrient media supplemented
with NaCl (0 or 150 mM) and/or SNP (0, 50, 100 or 250 μM) during one week treatment. After four
weeks, the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT)
and peroxidase (POX) was determined spectrophotometrically. Thus, through 14 different treatments,
the effect of NaCl and SNP on biochemical response of centaury shoots was investigated.
The results obtained in this work showed that NaCl decreased the activity of SOD, CAT and POX.
On the other hand, exogenous application of SNP increased SOD, CAT and POX activity in centaury
shoots after four weeks. It can be concluded that the effect of SNP on increased tolerance of
centaury shoots to salinity is reflected through the changed activity of enzymatic components of
antioxidant defence.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5327
ER  - 

@conference{
author = "Đurić, Marija and Stamenković, Nikola and Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2022",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant used in traditional medicine
since ancient times. Centaury inhabits dry pastures, roadsides and fields from lowlands to
mountain vegetation. Considering that centaury can also be found on moderately saline habitats
in nature, it was interesting to investigate the effect of exogenously applied sodium nitroprusside
(SNP), as a donor of nitrogen monoxide (NO), on antioxidative enzymes activity of centaury shoots
grown under stress caused by sodium chloride (NaCl) in vitro. Centaury shoots were grown on
½MS nutrient media containing graded concentrations of SNP (0, 50, 100 and 250 μM). After three
weeks of pretreatment, shoots were further transferred to ½MS nutrient media supplemented
with NaCl (0 or 150 mM) and/or SNP (0, 50, 100 or 250 μM) during one week treatment. After four
weeks, the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT)
and peroxidase (POX) was determined spectrophotometrically. Thus, through 14 different treatments,
the effect of NaCl and SNP on biochemical response of centaury shoots was investigated.
The results obtained in this work showed that NaCl decreased the activity of SOD, CAT and POX.
On the other hand, exogenous application of SNP increased SOD, CAT and POX activity in centaury
shoots after four weeks. It can be concluded that the effect of SNP on increased tolerance of
centaury shoots to salinity is reflected through the changed activity of enzymatic components of
antioxidant defence.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5327"
}

Đurić, M., Stamenković, N., Trifunović-Momčilov, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2022). The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 60.
https://hdl.handle.net/21.15107/rcub_ibiss_5327

Đurić M, Stamenković N, Trifunović-Momčilov M, Milošević S, Marković M, Giba Z, Subotić A. The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:60.
https://hdl.handle.net/21.15107/rcub_ibiss_5327 .

Đurić, Marija, Stamenković, Nikola, Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):60,
https://hdl.handle.net/21.15107/rcub_ibiss_5327 .

TY  - CONF
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Đurić, Marija
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5377
AB  - Кичица (Centaurium erythraea Rafn) је лековита биљна која се у народној медицини 
од давнина употребљава као лек за снижавање температуре, регулацију нивоа 
шећера у крви, а такође и за лечење анемије, жутице и гихта. У природи кичица је 
врло честа на сувим пашњацима, ободу путева и њива. Познато је да заслањене 
подлоге могу проузроковати прогресиван губитак хлорофила у листовима биљака. 
Такође је познато да су биљке које живе на заслањеним стаништима развиле 
механизме који им омогућавају да одрже фотосинтезу у присуству повишене 
концентрације соли. Имајући у виду да кичица у природи насељава и благо 
заслањена земљишта, испитиван је утицај егзогено примењеног натријум 
нитропрусида (SNP), као донора азот моноксида, на садржај фотосинтетичких 
пигмената изданака кичице гајених in vitro у условима стреса изазваног натријум 
хлоридом (NaCl). У овом раду приказан је позитиван утицај SNP на концентрацију 
укупних хлорофила у условима стреса изазваног NaCl. Најбоље резултате, у 
смислу повишеног нивоа укупног хлорофила у односу на контролне изданке гајене 
у присуству NaCl, показала је најнижа примењена концентрације SNP. Са друге 
стране резултати у овом раду показују да је SNP утицао на повећање биосинтезе 
каротеноида у условима стреса изазваног NaCl. Уочено је да је повећање 
концентрације укупних каротеноида у корелацији са примењеним концентрацијама 
SNP. На основу добијених резултата може се закључити да примена SNP има 
смисла када је кичица изложена стресу изазваном NaCl.
AB  - Kičica (Centaurium erythraea Rafn) je lekovita biljna koja se u narodnoj medicini od davnina upotrebljava kao lek za snižavanje temperature, regulaciju nivoa šećera u krvi, a takođe i za lečenje anemije, žutice i gihta. U prirodi kičica je vrlo česta na suvim pašnjacima, obodu puteva i njiva. Poznato je da zaslanjene podloge mogu prouzrokovati progresivan gubitak hlorofila u listovima biljaka. Takođe je poznato da su biljke koje žive na zaslanjenim staništima razvile mehanizme koji im omogućavaju da održe fotosintezu u prisustvu povišene koncentracije soli. Imajući u vidu da kičica u prirodi naseljava i blago zaslanjena zemljišta, ispitivan je uticaj egzogeno primenjenog natrijum nitroprusida (SNP), kao donora azot monoksida, na sadržaj fotosintetičkih pigmenata izdanaka kičice gajenih in vitro u uslovima stresa izazvanog natrijum hloridom (NaCl). U ovom radu prikazan je pozitivan uticaj SNP na koncentraciju ukupnih hlorofila u uslovima stresa izazvanog NaCl. Najbolje rezultate, u smislu povišenog nivoa ukupnog hlorofila u odnosu na kontrolne izdanke gajene u prisustvu NaCl, pokazala je najniža primenjena koncentracije SNP. Sa druge strane rezultati u ovom radu pokazuju da je SNP uticao na povećanje biosinteze karotenoida u uslovima stresa izazvanog NaCl. Uočeno je da je povećanje koncentracije ukupnih karotenoida u korelaciji sa primenjenim koncentracijama SNP. Na osnovu dobijenih rezultata može se zaključiti da primena SNP ima smisla kada je kičica izložena stresu izazvanom NaCl.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом
T1  - Uticaj natrijum nitroprusida na sadržaj fotosintetičkih pigmenata kičice (Centaurium erythraea Rafn) gajene in vitro u uslovima stresa izazvanog natrijum hloridom
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5377
ER  - 

@conference{
author = "Trifunović-Momčilov, Milana and Stamenković, Nikola and Đurić, Marija and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2022",
abstract = "Кичица (Centaurium erythraea Rafn) је лековита биљна која се у народној медицини 
од давнина употребљава као лек за снижавање температуре, регулацију нивоа 
шећера у крви, а такође и за лечење анемије, жутице и гихта. У природи кичица је 
врло честа на сувим пашњацима, ободу путева и њива. Познато је да заслањене 
подлоге могу проузроковати прогресиван губитак хлорофила у листовима биљака. 
Такође је познато да су биљке које живе на заслањеним стаништима развиле 
механизме који им омогућавају да одрже фотосинтезу у присуству повишене 
концентрације соли. Имајући у виду да кичица у природи насељава и благо 
заслањена земљишта, испитиван је утицај егзогено примењеног натријум 
нитропрусида (SNP), као донора азот моноксида, на садржај фотосинтетичких 
пигмената изданака кичице гајених in vitro у условима стреса изазваног натријум 
хлоридом (NaCl). У овом раду приказан је позитиван утицај SNP на концентрацију 
укупних хлорофила у условима стреса изазваног NaCl. Најбоље резултате, у 
смислу повишеног нивоа укупног хлорофила у односу на контролне изданке гајене 
у присуству NaCl, показала је најнижа примењена концентрације SNP. Са друге 
стране резултати у овом раду показују да је SNP утицао на повећање биосинтезе 
каротеноида у условима стреса изазваног NaCl. Уочено је да је повећање 
концентрације укупних каротеноида у корелацији са примењеним концентрацијама 
SNP. На основу добијених резултата може се закључити да примена SNP има 
смисла када је кичица изложена стресу изазваном NaCl., Kičica (Centaurium erythraea Rafn) je lekovita biljna koja se u narodnoj medicini od davnina upotrebljava kao lek za snižavanje temperature, regulaciju nivoa šećera u krvi, a takođe i za lečenje anemije, žutice i gihta. U prirodi kičica je vrlo česta na suvim pašnjacima, obodu puteva i njiva. Poznato je da zaslanjene podloge mogu prouzrokovati progresivan gubitak hlorofila u listovima biljaka. Takođe je poznato da su biljke koje žive na zaslanjenim staništima razvile mehanizme koji im omogućavaju da održe fotosintezu u prisustvu povišene koncentracije soli. Imajući u vidu da kičica u prirodi naseljava i blago zaslanjena zemljišta, ispitivan je uticaj egzogeno primenjenog natrijum nitroprusida (SNP), kao donora azot monoksida, na sadržaj fotosintetičkih pigmenata izdanaka kičice gajenih in vitro u uslovima stresa izazvanog natrijum hloridom (NaCl). U ovom radu prikazan je pozitivan uticaj SNP na koncentraciju ukupnih hlorofila u uslovima stresa izazvanog NaCl. Najbolje rezultate, u smislu povišenog nivoa ukupnog hlorofila u odnosu na kontrolne izdanke gajene u prisustvu NaCl, pokazala je najniža primenjena koncentracije SNP. Sa druge strane rezultati u ovom radu pokazuju da je SNP uticao na povećanje biosinteze karotenoida u uslovima stresa izazvanog NaCl. Uočeno je da je povećanje koncentracije ukupnih karotenoida u korelaciji sa primenjenim koncentracijama SNP. Na osnovu dobijenih rezultata može se zaključiti da primena SNP ima smisla kada je kičica izložena stresu izazvanom NaCl.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом, Uticaj natrijum nitroprusida na sadržaj fotosintetičkih pigmenata kičice (Centaurium erythraea Rafn) gajene in vitro u uslovima stresa izazvanog natrijum hloridom",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5377"
}

Trifunović-Momčilov, M., Stamenković, N., Đurić, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2022). Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 69.
https://hdl.handle.net/21.15107/rcub_ibiss_5377

Trifunović-Momčilov M, Stamenković N, Đurić M, Milošević S, Marković M, Giba Z, Subotić A. Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:69.
https://hdl.handle.net/21.15107/rcub_ibiss_5377 .

Trifunović-Momčilov, Milana, Stamenković, Nikola, Đurić, Marija, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):69,
https://hdl.handle.net/21.15107/rcub_ibiss_5377 .

TY  - CONF
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Marković, Marija
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5398
AB  - Лепи Јова (Impatiens walleriana) је веома популарна хортикултурна врста у Србији 
и свету због својих декоративних особина, дугог периода цветања и широког 
спектра боје цветова. У његовој комерцијалној производњи наводњавање је 
смањено током транспорта и пласмана биљака на тржиште. Суша има неповољан 
утицај на растење и развиће биљака, а у ћелијама доводи до успоравања 
анаболичких и убрзавања катаболичких процеса, уз појачану производњу 
реактивних форми кисеоника (Reactive oxygen species – ROS). Дисбаланс између 
настанка и уклањања ROS има за последицу нарушавање ћелијске хомеостазе и 
оксидативни стрес. Иако ROS имају важну сигналну улогу, њихова висока 
цитотоксичност и реактивност доводе до модификација DNK, RNK, протеина и 
липида мембрана. У овом истраживању испитиван је утицај натријум 
нитропрусида (Sodium nitroprusside – SNP), као донора NO, на физиолошке и 
биохемијске промене in vitro гајеног Лепог Јове у условима суше изазване 
полиетилен гликолом (PEG8000). Изданци (величине 20-25 мм) су гајени на 
хранљивим подлогама са SNP (0, 50, 100 и 250 μM) 20 дана. Након претретмана, 
изданци су пребачени на свеже MS подлоге којима су додати PEG8000 (0 или 3%) 
и/или SNP (0, 50, 100 или 250 μM) и гајени су седам дана. Утицај егзогене примене 
SNP на повећање толеранције Лепог Јове на сушу испољава се у променама 
концентрације H2O2, малондиалдехида и укупних фенолних једињења, као и 
активности актиоксидативних ензима: каталаза, пероксидаза и супероксиддисмутаза у изданцима.
AB  - Lepi Jova (Impatiens walleriana) je veoma popularna hortikulturna vrsta u Srbiji i svetu zbog svojih dekorativnih osobina, dugog perioda cvetanja i širokog spektra boje cvetova. U njegovoj komercijalnoj proizvodnji navodnjavanje je smanjeno tokom transporta i plasmana biljaka na tržište. Suša ima nepovoljan uticaj na rastenje i razviće biljaka, a u ćelijama dovodi do usporavanja anaboličkih i ubrzavanja kataboličkih procesa, uz pojačanu proizvodnju reaktivnih formi kiseonika (Reactive oxygen species – ROS). Disbalans između nastanka i uklanjanja ROS ima za posledicu narušavanje ćelijske homeostaze i oksidativni stres. Iako ROS imaju važnu signalnu ulogu, njihova visoka citotoksičnost i reaktivnost dovode do modifikacija DNK, RNK, proteina i lipida membrana. U ovom istraživanju ispitivan je uticaj natrijum nitroprusida (Sodium nitroprusside – SNP), kao donora NO, na fiziološke i biohemijske promene in vitro gajenog Lepog Jove u uslovima suše izazvane polietilen glikolom (PEG8000). Izdanci (veličine 20-25 mm) su gajeni na hranljivim podlogama sa SNP (0, 50, 100 i 250 μM) 20 dana. Nakon pretretmana, izdanci su prebačeni na sveže MS podloge kojima su dodati PEG8000 (0 ili 3%) i/ili SNP (0, 50, 100 ili 250 μM) i gajeni su sedam dana. Uticaj egzogene primene SNP na povećanje tolerancije Lepog Jove na sušu ispoljava se u promenama koncentracije H2O2, malondialdehida i ukupnih fenolnih jedinjenja, kao i aktivnosti aktioksidativnih enzima: katalaza, peroksidaza i superoksiddismutaza u izdancima.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro
T1  - Uticaj natrijum nitroprusida na fiziološki odgovor Lepog Jove (Impatiens walleriana) u uslovima suše izazvane polietilen glikolom in vitro
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5398
ER  - 

@conference{
author = "Milošević, Snežana and Đurić, Marija and Trifunović-Momčilov, Milana and Stamenković, Nikola and Marković, Marija and Subotić, Angelina",
year = "2022",
abstract = "Лепи Јова (Impatiens walleriana) је веома популарна хортикултурна врста у Србији 
и свету због својих декоративних особина, дугог периода цветања и широког 
спектра боје цветова. У његовој комерцијалној производњи наводњавање је 
смањено током транспорта и пласмана биљака на тржиште. Суша има неповољан 
утицај на растење и развиће биљака, а у ћелијама доводи до успоравања 
анаболичких и убрзавања катаболичких процеса, уз појачану производњу 
реактивних форми кисеоника (Reactive oxygen species – ROS). Дисбаланс између 
настанка и уклањања ROS има за последицу нарушавање ћелијске хомеостазе и 
оксидативни стрес. Иако ROS имају важну сигналну улогу, њихова висока 
цитотоксичност и реактивност доводе до модификација DNK, RNK, протеина и 
липида мембрана. У овом истраживању испитиван је утицај натријум 
нитропрусида (Sodium nitroprusside – SNP), као донора NO, на физиолошке и 
биохемијске промене in vitro гајеног Лепог Јове у условима суше изазване 
полиетилен гликолом (PEG8000). Изданци (величине 20-25 мм) су гајени на 
хранљивим подлогама са SNP (0, 50, 100 и 250 μM) 20 дана. Након претретмана, 
изданци су пребачени на свеже MS подлоге којима су додати PEG8000 (0 или 3%) 
и/или SNP (0, 50, 100 или 250 μM) и гајени су седам дана. Утицај егзогене примене 
SNP на повећање толеранције Лепог Јове на сушу испољава се у променама 
концентрације H2O2, малондиалдехида и укупних фенолних једињења, као и 
активности актиоксидативних ензима: каталаза, пероксидаза и супероксиддисмутаза у изданцима., Lepi Jova (Impatiens walleriana) je veoma popularna hortikulturna vrsta u Srbiji i svetu zbog svojih dekorativnih osobina, dugog perioda cvetanja i širokog spektra boje cvetova. U njegovoj komercijalnoj proizvodnji navodnjavanje je smanjeno tokom transporta i plasmana biljaka na tržište. Suša ima nepovoljan uticaj na rastenje i razviće biljaka, a u ćelijama dovodi do usporavanja anaboličkih i ubrzavanja kataboličkih procesa, uz pojačanu proizvodnju reaktivnih formi kiseonika (Reactive oxygen species – ROS). Disbalans između nastanka i uklanjanja ROS ima za posledicu narušavanje ćelijske homeostaze i oksidativni stres. Iako ROS imaju važnu signalnu ulogu, njihova visoka citotoksičnost i reaktivnost dovode do modifikacija DNK, RNK, proteina i lipida membrana. U ovom istraživanju ispitivan je uticaj natrijum nitroprusida (Sodium nitroprusside – SNP), kao donora NO, na fiziološke i biohemijske promene in vitro gajenog Lepog Jove u uslovima suše izazvane polietilen glikolom (PEG8000). Izdanci (veličine 20-25 mm) su gajeni na hranljivim podlogama sa SNP (0, 50, 100 i 250 μM) 20 dana. Nakon pretretmana, izdanci su prebačeni na sveže MS podloge kojima su dodati PEG8000 (0 ili 3%) i/ili SNP (0, 50, 100 ili 250 μM) i gajeni su sedam dana. Uticaj egzogene primene SNP na povećanje tolerancije Lepog Jove na sušu ispoljava se u promenama koncentracije H2O2, malondialdehida i ukupnih fenolnih jedinjenja, kao i aktivnosti aktioksidativnih enzima: katalaza, peroksidaza i superoksiddismutaza u izdancima.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro, Uticaj natrijum nitroprusida na fiziološki odgovor Lepog Jove (Impatiens walleriana) u uslovima suše izazvane polietilen glikolom in vitro",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5398"
}

Milošević, S., Đurić, M., Trifunović-Momčilov, M., Stamenković, N., Marković, M.,& Subotić, A.. (2022). Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_5398

Milošević S, Đurić M, Trifunović-Momčilov M, Stamenković N, Marković M, Subotić A. Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_5398 .

Milošević, Snežana, Đurić, Marija, Trifunović-Momčilov, Milana, Stamenković, Nikola, Marković, Marija, Subotić, Angelina, "Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):81,
https://hdl.handle.net/21.15107/rcub_ibiss_5398 .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2021
UR  - https://doi.org/10.3390/plants10050902
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4228
AB  - In ornamental geophytes, conventional vegetative propagation is not economically feasible due to very slow development and ineffective methods. It can take several years until a new plant is formed and commercial profitability is achieved. Therefore, micropropagation techniques have been developed to increase the multiplication rate and thus shorten the multiplication and regeneration period. The majority of these techniques rely on the formation of new bulbs and their sprouting. Dormancy is one of the main limiting factors to speed up multiplication in vitro. Bulbous species have a period of bulb dormancy which enables them to survive unfavorable natural conditions. Bulbs grown in vitro also exhibit dormancy, which has to be overcome in order to allow sprouting of bulbs in the next vegetation period. During the period of dormancy, numerous physiological processes occur, many of which have not been elucidated yet. Understanding the process of dormancy will allow us to speed up and improve breeding of geophytes and thereby achieve economic profitability, which is very important for horticulture. This review focuses on recent findings in the area of bulb dormancy initiation and release in fritillaries, with particular emphasis on the effect of plant growth regulators and low-temperature pretreatment on dormancy release in relation to induction of antioxidative enzymes’ activity in vitro.
PB  - MDPI AG
T2  - Plants
T1  - Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters
IS  - 5
VL  - 10
DO  - 10.3390/plants10050902
SP  - 902
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Jevremović, Slađana and Subotić, Angelina",
year = "2021",
abstract = "In ornamental geophytes, conventional vegetative propagation is not economically feasible due to very slow development and ineffective methods. It can take several years until a new plant is formed and commercial profitability is achieved. Therefore, micropropagation techniques have been developed to increase the multiplication rate and thus shorten the multiplication and regeneration period. The majority of these techniques rely on the formation of new bulbs and their sprouting. Dormancy is one of the main limiting factors to speed up multiplication in vitro. Bulbous species have a period of bulb dormancy which enables them to survive unfavorable natural conditions. Bulbs grown in vitro also exhibit dormancy, which has to be overcome in order to allow sprouting of bulbs in the next vegetation period. During the period of dormancy, numerous physiological processes occur, many of which have not been elucidated yet. Understanding the process of dormancy will allow us to speed up and improve breeding of geophytes and thereby achieve economic profitability, which is very important for horticulture. This review focuses on recent findings in the area of bulb dormancy initiation and release in fritillaries, with particular emphasis on the effect of plant growth regulators and low-temperature pretreatment on dormancy release in relation to induction of antioxidative enzymes’ activity in vitro.",
publisher = "MDPI AG",
journal = "Plants",
title = "Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters",
number = "5",
volume = "10",
doi = "10.3390/plants10050902",
pages = "902"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Jevremović, S.,& Subotić, A.. (2021). Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters. in Plants
MDPI AG., 10(5), 902.
https://doi.org/10.3390/plants10050902

Marković M, Trifunović-Momčilov M, Uzelac B, Jevremović S, Subotić A. Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters. in Plants. 2021;10(5):902.
doi:10.3390/plants10050902 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Jevremović, Slađana, Subotić, Angelina, "Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters" in Plants, 10, no. 5 (2021):902,
https://doi.org/10.3390/plants10050902 . .

TY  - CONF
AU  - Radulović, Olga
AU  - Gašić, Uroš
AU  - Marković, Marija
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5666
AB  - The common duckweed (Lemna minor L.) as a model organism is experiencing a form of a renaissance. In this study, our focus was on the interactions between duckweeds and a rhizosphere-associated bacterial strain, Klebsiella oxytoca (Access. No. MK212915). Five distinct phenolic compounds were identified by liquid chromatography–mass spectrometry: luteolin 6,8-di-C-hexoside, p-hydroxybenzoic acid, caffeic acid, apigenin 6-C-(2′′-pentosyl)hexoside and p-coumaric acid. All of the identified compounds reflect the colonization of the plant by K. oxytoca. This paper is another call for all plant physiologists to focus their research on L. minor and to analyze different aspects of complex plant/bacterium interactions.
PB  - Basel: MDPI
C3  - The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online
T1  - Phenolic Compounds as Biomarkers of Interactions between the Endophyte Klebsiella oxytoca and the Common Duckweed, Lemna minor L.
DO  - 10.3390/IECPS2021-11924
SP  - 52
ER  - 

@conference{
author = "Radulović, Olga and Gašić, Uroš and Marković, Marija",
year = "2021",
abstract = "The common duckweed (Lemna minor L.) as a model organism is experiencing a form of a renaissance. In this study, our focus was on the interactions between duckweeds and a rhizosphere-associated bacterial strain, Klebsiella oxytoca (Access. No. MK212915). Five distinct phenolic compounds were identified by liquid chromatography–mass spectrometry: luteolin 6,8-di-C-hexoside, p-hydroxybenzoic acid, caffeic acid, apigenin 6-C-(2′′-pentosyl)hexoside and p-coumaric acid. All of the identified compounds reflect the colonization of the plant by K. oxytoca. This paper is another call for all plant physiologists to focus their research on L. minor and to analyze different aspects of complex plant/bacterium interactions.",
publisher = "Basel: MDPI",
journal = "The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online",
title = "Phenolic Compounds as Biomarkers of Interactions between the Endophyte Klebsiella oxytoca and the Common Duckweed, Lemna minor L.",
doi = "10.3390/IECPS2021-11924",
pages = "52"
}

Radulović, O., Gašić, U.,& Marković, M.. (2021). Phenolic Compounds as Biomarkers of Interactions between the Endophyte Klebsiella oxytoca and the Common Duckweed, Lemna minor L.. in The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online
Basel: MDPI., 52.
https://doi.org/10.3390/IECPS2021-11924

Radulović O, Gašić U, Marković M. Phenolic Compounds as Biomarkers of Interactions between the Endophyte Klebsiella oxytoca and the Common Duckweed, Lemna minor L.. in The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online. 2021;:52.
doi:10.3390/IECPS2021-11924 .

Radulović, Olga, Gašić, Uroš, Marković, Marija, "Phenolic Compounds as Biomarkers of Interactions between the Endophyte Klebsiella oxytoca and the Common Duckweed, Lemna minor L." in The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online (2021):52,
https://doi.org/10.3390/IECPS2021-11924 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Marković, Marija
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2021
UR  - https://www.nature.com/articles/s41598-021-85932-w
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4194
AB  - Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.
PB  - Springer Science and Business Media LLC
T2  - Scientific Reports
T1  - Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-85932-w
SP  - 6494
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Václav and Savić, Jelena and Raspor, Martin and Marković, Marija and Dobrev, Petre I. and Ninković, Slavica",
year = "2021",
abstract = "Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.",
publisher = "Springer Science and Business Media LLC",
journal = "Scientific Reports",
title = "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-85932-w",
pages = "6494"
}

Ćosić, T., Motyka, V., Savić, J., Raspor, M., Marković, M., Dobrev, P. I.,& Ninković, S.. (2021). Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports
Springer Science and Business Media LLC., 11(1), 6494.
https://doi.org/10.1038/s41598-021-85932-w

Ćosić T, Motyka V, Savić J, Raspor M, Marković M, Dobrev PI, Ninković S. Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports. 2021;11(1):6494.
doi:10.1038/s41598-021-85932-w .

Ćosić, Tatjana, Motyka, Václav, Savić, Jelena, Raspor, Martin, Marković, Marija, Dobrev, Petre I., Ninković, Slavica, "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi" in Scientific Reports, 11, no. 1 (2021):6494,
https://doi.org/10.1038/s41598-021-85932-w . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana Č.
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4501
AB  - The effects of graded sodium chloride (NaCl) concentrations (0-, 50-, 100-, 150-, and 200-
mM) on photosynthetic pigment contents in in vitro grown shoots of important medicinal plant
species (Centaurium erythraea Rafn) were investigated. Non-transformed, one AtCKX1 and two
AtCKX2 transgenic centaury lines, with altered cytokinin profiles, were used in this study. The chlorophyll
(Chl) and carotenoid contents differed in the non-transformed and transgenic lines. In general,
salinity significantly reduced the Chl a and Chl b contents in comparison to the NaCl-free medium.
The lowest Chl content was observed in AtCKX2 transgenic shoots grown on all the culture
media. The total carotenoid content was increased in shoots of non-transformed and both AtCKX2
transgenic lines grown in 50-mM NaCl. On the other hand, in concentrations ˃50-mM NaCl, the
total carotenoid content was decreased in all analysed centaury shoots. The Chl a/Chl b ratio in all
the shoots increased progressively in the graded NaCl concentrations. Contrarily, the addition of
NaCl in the culture medium reduced the Chl/carotenoid ratio in centaury shoots. Taken together,
the results of this study partly explained the mode of centaury plant adaptations to salt stress in
vitro. Thus, the results on centaury shoots confirmed that the determination of the photosynthetic
pigment contents can be a very useful non-destructive screening method in order to discriminate
susceptible and resistant plant species/lines to salt stress conditions.
PB  - Basel: MDPI
T2  - Agronomy
T1  - Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro
IS  - 10
VL  - 11
DO  - 10.3390/agronomy11102056
SP  - 2056
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Đurić, Marija and Jevremović, Slađana and Dragićević, Ivana Č. and Subotić, Angelina",
year = "2021",
abstract = "The effects of graded sodium chloride (NaCl) concentrations (0-, 50-, 100-, 150-, and 200-
mM) on photosynthetic pigment contents in in vitro grown shoots of important medicinal plant
species (Centaurium erythraea Rafn) were investigated. Non-transformed, one AtCKX1 and two
AtCKX2 transgenic centaury lines, with altered cytokinin profiles, were used in this study. The chlorophyll
(Chl) and carotenoid contents differed in the non-transformed and transgenic lines. In general,
salinity significantly reduced the Chl a and Chl b contents in comparison to the NaCl-free medium.
The lowest Chl content was observed in AtCKX2 transgenic shoots grown on all the culture
media. The total carotenoid content was increased in shoots of non-transformed and both AtCKX2
transgenic lines grown in 50-mM NaCl. On the other hand, in concentrations ˃50-mM NaCl, the
total carotenoid content was decreased in all analysed centaury shoots. The Chl a/Chl b ratio in all
the shoots increased progressively in the graded NaCl concentrations. Contrarily, the addition of
NaCl in the culture medium reduced the Chl/carotenoid ratio in centaury shoots. Taken together,
the results of this study partly explained the mode of centaury plant adaptations to salt stress in
vitro. Thus, the results on centaury shoots confirmed that the determination of the photosynthetic
pigment contents can be a very useful non-destructive screening method in order to discriminate
susceptible and resistant plant species/lines to salt stress conditions.",
publisher = "Basel: MDPI",
journal = "Agronomy",
title = "Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro",
number = "10",
volume = "11",
doi = "10.3390/agronomy11102056",
pages = "2056"
}

Trifunović-Momčilov, M., Milošević, S., Marković, M., Đurić, M., Jevremović, S., Dragićević, I. Č.,& Subotić, A.. (2021). Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro. in Agronomy
Basel: MDPI., 11(10), 2056.
https://doi.org/10.3390/agronomy11102056

Trifunović-Momčilov M, Milošević S, Marković M, Đurić M, Jevremović S, Dragićević IČ, Subotić A. Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro. in Agronomy. 2021;11(10):2056.
doi:10.3390/agronomy11102056 .

Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Đurić, Marija, Jevremović, Slađana, Dragićević, Ivana Č., Subotić, Angelina, "Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro" in Agronomy, 11, no. 10 (2021):2056,
https://doi.org/10.3390/agronomy11102056 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Václav
AU  - Dobrev, Petre I.
AU  - Marković, Marija
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana Č.
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4649
AB  - Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.
PB  - Springer Nature
T2  - Scientific Reports
T1  - Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro
VL  - 11
DO  - 10.1038/s41598-021-00866-7
SP  - 21471
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Motyka, Václav and Dobrev, Petre I. and Marković, Marija and Milošević, Snežana and Jevremović, Slađana and Dragićević, Ivana Č. and Subotić, Angelina",
year = "2021",
abstract = "Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro",
volume = "11",
doi = "10.1038/s41598-021-00866-7",
pages = "21471"
}

Trifunović-Momčilov, M., Motyka, V., Dobrev, P. I., Marković, M., Milošević, S., Jevremović, S., Dragićević, I. Č.,& Subotić, A.. (2021). Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro. in Scientific Reports
Springer Nature., 11, 21471.
https://doi.org/10.1038/s41598-021-00866-7

Trifunović-Momčilov M, Motyka V, Dobrev PI, Marković M, Milošević S, Jevremović S, Dragićević IČ, Subotić A. Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro. in Scientific Reports. 2021;11:21471.
doi:10.1038/s41598-021-00866-7 .

Trifunović-Momčilov, Milana, Motyka, Václav, Dobrev, Petre I., Marković, Marija, Milošević, Snežana, Jevremović, Slađana, Dragićević, Ivana Č., Subotić, Angelina, "Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro" in Scientific Reports, 11 (2021):21471,
https://doi.org/10.1038/s41598-021-00866-7 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Stanojević, Olja
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
PY  - 2021
UR  - https://www.mdpi.com/2076-3921/10/11/1719
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4653
AB  - Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3
IS  - 11
VL  - 10
DO  - 10.3390/antiox10111719
SP  - 1719
ER  - 

@article{
author = "Radulović, Olga and Stanković, Slaviša and Stanojević, Olja and Vujčić, Zoran and Dojnov, Biljana and Trifunović-Momčilov, Milana and Marković, Marija",
year = "2021",
abstract = "Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3",
number = "11",
volume = "10",
doi = "10.3390/antiox10111719",
pages = "1719"
}

Radulović, O., Stanković, S., Stanojević, O., Vujčić, Z., Dojnov, B., Trifunović-Momčilov, M.,& Marković, M.. (2021). Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3. in Antioxidants
Basel: MDPI., 10(11), 1719.
https://doi.org/10.3390/antiox10111719

Radulović O, Stanković S, Stanojević O, Vujčić Z, Dojnov B, Trifunović-Momčilov M, Marković M. Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3. in Antioxidants. 2021;10(11):1719.
doi:10.3390/antiox10111719 .

Radulović, Olga, Stanković, Slaviša, Stanojević, Olja, Vujčić, Zoran, Dojnov, Biljana, Trifunović-Momčilov, Milana, Marković, Marija, "Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3" in Antioxidants, 10, no. 11 (2021):1719,
https://doi.org/10.3390/antiox10111719 . .

TY  - JOUR
AU  - Simonović, Ana
AU  - Trifunović-Momčilov, Milana
AU  - Filipović, Biljana
AU  - Marković, Marija
AU  - Bogdanović, Milica
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4088
AB  - Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum
of secondary metabolites with confirmed healing properties. Centaury is an emerging model in
plant developmental biology due to its vigorous regenerative potential and great developmental
plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic
embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions
to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic
embryos. When SE is initiated from centaury root explants, the process occurs spontaneously
(on hormone-free medium), directly (without the callusing phase), and the somatic embryos are
of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is
indirect (preceded by callusing). Histological observations and culture conditions are compared
in these two systems. The changes in antioxidative enzymes were followed during SE from the
leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were
analyzed using a variety of approaches. The newest and preliminary results, including centaury
transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed
as perspectives of centaury research.
PB  - MDPI, Basel, Switzerland
T2  - Plants
T1  - Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review
VL  - 10
DO  - 10.3390/plants10010070
SP  - 70
ER  - 

@article{
author = "Simonović, Ana and Trifunović-Momčilov, Milana and Filipović, Biljana and Marković, Marija and Bogdanović, Milica and Subotić, Angelina",
year = "2021",
abstract = "Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum
of secondary metabolites with confirmed healing properties. Centaury is an emerging model in
plant developmental biology due to its vigorous regenerative potential and great developmental
plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic
embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions
to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic
embryos. When SE is initiated from centaury root explants, the process occurs spontaneously
(on hormone-free medium), directly (without the callusing phase), and the somatic embryos are
of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is
indirect (preceded by callusing). Histological observations and culture conditions are compared
in these two systems. The changes in antioxidative enzymes were followed during SE from the
leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were
analyzed using a variety of approaches. The newest and preliminary results, including centaury
transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed
as perspectives of centaury research.",
publisher = "MDPI, Basel, Switzerland",
journal = "Plants",
title = "Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review",
volume = "10",
doi = "10.3390/plants10010070",
pages = "70"
}

Simonović, A., Trifunović-Momčilov, M., Filipović, B., Marković, M., Bogdanović, M.,& Subotić, A.. (2021). Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review. in Plants
MDPI, Basel, Switzerland., 10, 70.
https://doi.org/10.3390/plants10010070

Simonović A, Trifunović-Momčilov M, Filipović B, Marković M, Bogdanović M, Subotić A. Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review. in Plants. 2021;10:70.
doi:10.3390/plants10010070 .

Simonović, Ana, Trifunović-Momčilov, Milana, Filipović, Biljana, Marković, Marija, Bogdanović, Milica, Subotić, Angelina, "Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review" in Plants, 10 (2021):70,
https://doi.org/10.3390/plants10010070 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Paunović, Danijela
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12593
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3690
AB  - Common centaury (Centaurium erythraea Rafn.) is a plant species that can inhabit saline soils. It is known as a plant with high spontaneous regeneration potential in vitro. In the present work we evaluated shoots and roots salinity tolerance of non-transformed and three AtCKX transgenic centaury lines to graded NaCl concentrations (0, 50, 100, 150, 200 mM) in vitro. Overexpression of AtCKX genes in transgenic centaury plants resulted in an altered cytokinins (CKs) profile leading to a decline of bioactive CK levels and, at the same time, increased contents of storage CK forms, inactive CK forms and/or CK nucleotides. Significant increment of fresh shoot weight was obtained in shoots of non-transformed and AtCKX1 transgenic line only on medium supplemented with 50 mM NaCl. However two analysed AtCKX2 transgenic lines reduced shoot growth at all NaCl concentrations. In general, centaury roots showed higher tolerance to salinity than shoots. Non-transformed and AtCKX1 transgenic lines tolerated up to 100 mM NaCl without change in frequency of regeneration and number of regenerated plants. Roots of two analysed AtCKX2 transgenic lines showed different regeneration potential under salt stress. Regeneration of transgenic AtCKX2-26 shoots even at 200 mM NaCl was recorded. Salinity stress response of centaury shoots and roots was also evaluated at biochemical level. Free proline, malondialdehyde and hydrogen peroxide content as well as antioxidative enzymes activities were investigated in shoots and roots after 1, 2, 4 and 8 weeks. In general, adition of NaCl in culture medium elevated all biochemical parameters in centaury shoots and in roots. Considering that all analysed AtCKX transgenic centaury lines showed altered salt tolerance to graded NaCl concentrations in vitro it can be assumed that CKs might be involved in plant defence to salt stress conditions.
PB  - Blackwell Publishing Ltd
T2  - Annals of Applied Biology
T1  - Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro
DO  - 10.1111/aab.12593
SP  - aab.12593
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Paunović, Danijela and Milošević, Snežana and Marković, Marija and Jevremović, Slađana and Dragićević, Ivana and Subotić, Angelina",
year = "2020",
abstract = "Common centaury (Centaurium erythraea Rafn.) is a plant species that can inhabit saline soils. It is known as a plant with high spontaneous regeneration potential in vitro. In the present work we evaluated shoots and roots salinity tolerance of non-transformed and three AtCKX transgenic centaury lines to graded NaCl concentrations (0, 50, 100, 150, 200 mM) in vitro. Overexpression of AtCKX genes in transgenic centaury plants resulted in an altered cytokinins (CKs) profile leading to a decline of bioactive CK levels and, at the same time, increased contents of storage CK forms, inactive CK forms and/or CK nucleotides. Significant increment of fresh shoot weight was obtained in shoots of non-transformed and AtCKX1 transgenic line only on medium supplemented with 50 mM NaCl. However two analysed AtCKX2 transgenic lines reduced shoot growth at all NaCl concentrations. In general, centaury roots showed higher tolerance to salinity than shoots. Non-transformed and AtCKX1 transgenic lines tolerated up to 100 mM NaCl without change in frequency of regeneration and number of regenerated plants. Roots of two analysed AtCKX2 transgenic lines showed different regeneration potential under salt stress. Regeneration of transgenic AtCKX2-26 shoots even at 200 mM NaCl was recorded. Salinity stress response of centaury shoots and roots was also evaluated at biochemical level. Free proline, malondialdehyde and hydrogen peroxide content as well as antioxidative enzymes activities were investigated in shoots and roots after 1, 2, 4 and 8 weeks. In general, adition of NaCl in culture medium elevated all biochemical parameters in centaury shoots and in roots. Considering that all analysed AtCKX transgenic centaury lines showed altered salt tolerance to graded NaCl concentrations in vitro it can be assumed that CKs might be involved in plant defence to salt stress conditions.",
publisher = "Blackwell Publishing Ltd",
journal = "Annals of Applied Biology",
title = "Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro",
doi = "10.1111/aab.12593",
pages = "aab.12593"
}

Trifunović-Momčilov, M., Paunović, D., Milošević, S., Marković, M., Jevremović, S., Dragićević, I.,& Subotić, A.. (2020). Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro. in Annals of Applied Biology
Blackwell Publishing Ltd., aab.12593.
https://doi.org/10.1111/aab.12593

Trifunović-Momčilov M, Paunović D, Milošević S, Marković M, Jevremović S, Dragićević I, Subotić A. Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro. in Annals of Applied Biology. 2020;:aab.12593.
doi:10.1111/aab.12593 .

Trifunović-Momčilov, Milana, Paunović, Danijela, Milošević, Snežana, Marković, Marija, Jevremović, Slađana, Dragićević, Ivana, Subotić, Angelina, "Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro" in Annals of Applied Biology (2020):aab.12593,
https://doi.org/10.1111/aab.12593 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Radulović, Olga
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1573
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4010
AB  - The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.
PB  - MDPI AG
T2  - Plants
T1  - Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs
IS  - 11
VL  - 9
DO  - 10.3390/plants9111573
SP  - 1573
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Radulović, Olga and Milošević, Snežana and Jevremović, Slađana and Subotić, Angelina",
year = "2020",
abstract = "The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.",
publisher = "MDPI AG",
journal = "Plants",
title = "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs",
number = "11",
volume = "9",
doi = "10.3390/plants9111573",
pages = "1573"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Radulović, O., Milošević, S., Jevremović, S.,& Subotić, A.. (2020). Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. in Plants
MDPI AG., 9(11), 1573.
https://doi.org/10.3390/plants9111573

Marković M, Trifunović-Momčilov M, Uzelac B, Radulović O, Milošević S, Jevremović S, Subotić A. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. in Plants. 2020;9(11):1573.
doi:10.3390/plants9111573 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Radulović, Olga, Milošević, Snežana, Jevremović, Slađana, Subotić, Angelina, "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs" in Plants, 9, no. 11 (2020):1573,
https://doi.org/10.3390/plants9111573 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Cingel, Aleksandar
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1449
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3996
AB  - Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors-ancymidol (A) and paclobutrazol (P)-the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb's dormancy and its sprouting.
PB  - MDPI AG
T2  - Plants (Basel, Switzerland)
T1  - Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.
IS  - 11
VL  - 9
DO  - 10.3390/plants9111449
SP  - 1449
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Cingel, Aleksandar and Milošević, Snežana and Jevremović, Slađana and Subotić, Angelina",
year = "2020",
abstract = "Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors-ancymidol (A) and paclobutrazol (P)-the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb's dormancy and its sprouting.",
publisher = "MDPI AG",
journal = "Plants (Basel, Switzerland)",
title = "Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.",
number = "11",
volume = "9",
doi = "10.3390/plants9111449",
pages = "1449"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Cingel, A., Milošević, S., Jevremović, S.,& Subotić, A.. (2020). Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.. in Plants (Basel, Switzerland)
MDPI AG., 9(11), 1449.
https://doi.org/10.3390/plants9111449

Marković M, Trifunović-Momčilov M, Uzelac B, Cingel A, Milošević S, Jevremović S, Subotić A. Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.. in Plants (Basel, Switzerland). 2020;9(11):1449.
doi:10.3390/plants9111449 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Cingel, Aleksandar, Milošević, Snežana, Jevremović, Slađana, Subotić, Angelina, "Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content." in Plants (Basel, Switzerland), 9, no. 11 (2020):1449,
https://doi.org/10.3390/plants9111449 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Uzelac, Branka
AU  - Tadić, Vojin
AU  - Trifunović-Momčilov, Milana
AU  - Lozo, Jelena
AU  - Marković, Marija
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/5/599
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3676
AB  - The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.
PB  - MDPI AG
T2  - Plants
T1  - Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations
IS  - 5
VL  - 9
DO  - 10.3390/plants9050599
SP  - 599
ER  - 

@article{
author = "Radulović, Olga and Stanković, Slaviša and Uzelac, Branka and Tadić, Vojin and Trifunović-Momčilov, Milana and Lozo, Jelena and Marković, Marija",
year = "2020",
abstract = "The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.",
publisher = "MDPI AG",
journal = "Plants",
title = "Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations",
number = "5",
volume = "9",
doi = "10.3390/plants9050599",
pages = "599"
}

Radulović, O., Stanković, S., Uzelac, B., Tadić, V., Trifunović-Momčilov, M., Lozo, J.,& Marković, M.. (2020). Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. in Plants
MDPI AG., 9(5), 599.
https://doi.org/10.3390/plants9050599

Radulović O, Stanković S, Uzelac B, Tadić V, Trifunović-Momčilov M, Lozo J, Marković M. Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. in Plants. 2020;9(5):599.
doi:10.3390/plants9050599 .

Radulović, Olga, Stanković, Slaviša, Uzelac, Branka, Tadić, Vojin, Trifunović-Momčilov, Milana, Lozo, Jelena, Marković, Marija, "Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations" in Plants, 9, no. 5 (2020):599,
https://doi.org/10.3390/plants9050599 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Petrić, Marija
AU  - Raspor, Martin
AU  - Stanojević, Olja
AU  - Janakiev, Tamara
AU  - Tadić, Vojin
AU  - Stanković, Slaviša
PY  - 2019
UR  - http://www.pjoes.com/doi/10.15244/pjoes/81687
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3255
AB  - In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.
T2  - Polish Journal of Environmental Studies
T1  - Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II
IS  - 2
VL  - 28
DO  - 10.15244/pjoes/81687
SP  - 811
EP  - 822
ER  - 

@article{
author = "Radulović, Olga and Petrić, Marija and Raspor, Martin and Stanojević, Olja and Janakiev, Tamara and Tadić, Vojin and Stanković, Slaviša",
year = "2019",
abstract = "In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.",
journal = "Polish Journal of Environmental Studies",
title = "Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II",
number = "2",
volume = "28",
doi = "10.15244/pjoes/81687",
pages = "811-822"
}

Radulović, O., Petrić, M., Raspor, M., Stanojević, O., Janakiev, T., Tadić, V.,& Stanković, S.. (2019). Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II. in Polish Journal of Environmental Studies, 28(2), 811-822.
https://doi.org/10.15244/pjoes/81687

Radulović O, Petrić M, Raspor M, Stanojević O, Janakiev T, Tadić V, Stanković S. Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II. in Polish Journal of Environmental Studies. 2019;28(2):811-822.
doi:10.15244/pjoes/81687 .

Radulović, Olga, Petrić, Marija, Raspor, Martin, Stanojević, Olja, Janakiev, Tamara, Tadić, Vojin, Stanković, Slaviša, "Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II" in Polish Journal of Environmental Studies, 28, no. 2 (2019):811-822,
https://doi.org/10.15244/pjoes/81687 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Petrić, Marija
AU  - Raspor, Martin
AU  - Tadić, Vojin
AU  - Jovanović, Petar
AU  - Zečević, Vladan
PY  - 2019
UR  - http://www.journalssystem.com/pjoes/Assessment-of-in-vitro-multiplication-of-Lemna-minor-in-the-presence-of-phenol-plant,84921,0,2.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3251
AB  - The aim of this work was to examine the multiplication of the common duckweed (Lemna minor), an aquatic plant species widespread in European stagnant waters, in two different media (Murashige – Skoog and Hoagland) with and without phenol supplementation. In order to quantify plant multiplication we have used relative growth rate and tolerance indices on both tested media and at five phenol concentrations (10, 15, 20, 30 and 100 mg/L). Furthermore, we examined the possibility of phenol removal from aqueous media containing different phenol concentrations, by using plant/bacteria system consisting of the duckweed and its naturally occurring microbial populations. After 7 days, number of newly formed fronds was approximately four times higher than at the beginning of the experiment on both tested media. The most important result in this study was removal of 70% of phenol from the highest initial concentration of 100 mg/L, in mixed cultures of duckweed and bacteria. By comparison, aseptic duckweed cultures removed approximately 50% of phenol at the same initial concentration. Our duckweed specimen showed a fast reproduction rate, high tolerance to phenol and a possible cooperation with rhizosphere-associated bacteria. All of these traits can be ultimately utilized for bioremediation purposes.
T2  - Polish Journal of Environmental Studies
T1  - Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I
IS  - 2
VL  - 28
DO  - 10.15244/pjoes/84921
SP  - 803
EP  - 809
ER  - 

@article{
author = "Radulović, Olga and Petrić, Marija and Raspor, Martin and Tadić, Vojin and Jovanović, Petar and Zečević, Vladan",
year = "2019",
abstract = "The aim of this work was to examine the multiplication of the common duckweed (Lemna minor), an aquatic plant species widespread in European stagnant waters, in two different media (Murashige – Skoog and Hoagland) with and without phenol supplementation. In order to quantify plant multiplication we have used relative growth rate and tolerance indices on both tested media and at five phenol concentrations (10, 15, 20, 30 and 100 mg/L). Furthermore, we examined the possibility of phenol removal from aqueous media containing different phenol concentrations, by using plant/bacteria system consisting of the duckweed and its naturally occurring microbial populations. After 7 days, number of newly formed fronds was approximately four times higher than at the beginning of the experiment on both tested media. The most important result in this study was removal of 70% of phenol from the highest initial concentration of 100 mg/L, in mixed cultures of duckweed and bacteria. By comparison, aseptic duckweed cultures removed approximately 50% of phenol at the same initial concentration. Our duckweed specimen showed a fast reproduction rate, high tolerance to phenol and a possible cooperation with rhizosphere-associated bacteria. All of these traits can be ultimately utilized for bioremediation purposes.",
journal = "Polish Journal of Environmental Studies",
title = "Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I",
number = "2",
volume = "28",
doi = "10.15244/pjoes/84921",
pages = "803-809"
}

Radulović, O., Petrić, M., Raspor, M., Tadić, V., Jovanović, P.,& Zečević, V.. (2019). Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I. in Polish Journal of Environmental Studies, 28(2), 803-809.
https://doi.org/10.15244/pjoes/84921

Radulović O, Petrić M, Raspor M, Tadić V, Jovanović P, Zečević V. Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I. in Polish Journal of Environmental Studies. 2019;28(2):803-809.
doi:10.15244/pjoes/84921 .

Radulović, Olga, Petrić, Marija, Raspor, Martin, Tadić, Vojin, Jovanović, Petar, Zečević, Vladan, "Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I" in Polish Journal of Environmental Studies, 28, no. 2 (2019):803-809,
https://doi.org/10.15244/pjoes/84921 . .

TY  - CONF
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Vaclav
AU  - Dragićević, Ivana
AU  - Paunović, Danijela
AU  - Marković, Marija
AU  - Subotić, Angelina
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6322
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant that can inhabit saline soils.
Previous in vitro studies revealed centaury’s tolerance to salinity concerning shoot regeneration
and development. Centaury is known to spontaneously regenerate shoots from root segments
in vitro on hormone-free medium, probably due to endogenous levels of cytokinins (CKs) and
indolyl-3-acetic acid and their balance in roots. In this study we have investigated in vitro shoot
regeneration from root segments, i.e. frequency of shoot regeneration, the average number of regenerated shoots per explant and shoot biomass production in three transformed centaury lines,
bearing AtCKX1 or AtCKX2 genes. Graded concentrations of NaCl (0–200 mM) were added to the
nutrition medium during 8-week cultivation period. As previously shown, selected transformed
centaury lines grown in vitro were characterized by differently reduced bioactive CK contents, as a
consequence of different expression of transgenes. The main goal was to elucidate if changed CK
homeostasis interferes with salt-tolerance and affects shoot regeneration in centaury. The selected AtCKX centaury lines significantly differed from each other in terms of capacity for spontaneous
shoot regeneration and biomass production in vitro. NaCl lowered shoot regeneration in all of
the investigated lines, although they differed in their sensitivity to salt. Both AtCKX2 lines showed
increased sensitivity to NaCl with the highest sensitivity found in one with the highest potential for
spontaneous regeneration (AtCKX2-26). Concentration of 50 mM NaCl enhanced biomass production only in control and line AtCKX1-29. Our results indicate connection between CK homeostasis,
regeneration capacity and tolerance to salinity.
PB  - elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6322
ER  - 

@conference{
author = "Trifunović-Momčilov, Milana and Motyka, Vaclav and Dragićević, Ivana and Paunović, Danijela and Marković, Marija and Subotić, Angelina",
year = "2018",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant that can inhabit saline soils.
Previous in vitro studies revealed centaury’s tolerance to salinity concerning shoot regeneration
and development. Centaury is known to spontaneously regenerate shoots from root segments
in vitro on hormone-free medium, probably due to endogenous levels of cytokinins (CKs) and
indolyl-3-acetic acid and their balance in roots. In this study we have investigated in vitro shoot
regeneration from root segments, i.e. frequency of shoot regeneration, the average number of regenerated shoots per explant and shoot biomass production in three transformed centaury lines,
bearing AtCKX1 or AtCKX2 genes. Graded concentrations of NaCl (0–200 mM) were added to the
nutrition medium during 8-week cultivation period. As previously shown, selected transformed
centaury lines grown in vitro were characterized by differently reduced bioactive CK contents, as a
consequence of different expression of transgenes. The main goal was to elucidate if changed CK
homeostasis interferes with salt-tolerance and affects shoot regeneration in centaury. The selected AtCKX centaury lines significantly differed from each other in terms of capacity for spontaneous
shoot regeneration and biomass production in vitro. NaCl lowered shoot regeneration in all of
the investigated lines, although they differed in their sensitivity to salt. Both AtCKX2 lines showed
increased sensitivity to NaCl with the highest sensitivity found in one with the highest potential for
spontaneous regeneration (AtCKX2-26). Concentration of 50 mM NaCl enhanced biomass production only in control and line AtCKX1-29. Our results indicate connection between CK homeostasis,
regeneration capacity and tolerance to salinity.",
publisher = "elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6322"
}

Trifunović-Momčilov, M., Motyka, V., Dragićević, I., Paunović, D., Marković, M.,& Subotić, A.. (2018). Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology., 33.
https://hdl.handle.net/21.15107/rcub_ibiss_6322

Trifunović-Momčilov M, Motyka V, Dragićević I, Paunović D, Marković M, Subotić A. Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:33.
https://hdl.handle.net/21.15107/rcub_ibiss_6322 .

Trifunović-Momčilov, Milana, Motyka, Vaclav, Dragićević, Ivana, Paunović, Danijela, Marković, Marija, Subotić, Angelina, "Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):33,
https://hdl.handle.net/21.15107/rcub_ibiss_6322 .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Petrić, Marija
AU  - Uzelac, Branka
AU  - Milošević, Snežana
AU  - Vujčić, Zoran
AU  - Stevanović, Jasmina
AU  - Tadić, Jovan
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0304423817307483
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2956
AB  - We investigated the removal of phenol from water solutions (200 mg L−1) using two varieties of lettuce (Lactuca sativa L.) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L−1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L−1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L−1, but were not able to remove phenol at constant concentration above 25 mg L−1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.
T2  - Scientia Horticulturae
T1  - Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1
VL  - 231
DO  - 10.1016/j.scienta.2017.12.025
SP  - 210
EP  - 218
ER  - 

@article{
author = "Tadić, Vojin and Petrić, Marija and Uzelac, Branka and Milošević, Snežana and Vujčić, Zoran and Stevanović, Jasmina and Tadić, Jovan",
year = "2018",
abstract = "We investigated the removal of phenol from water solutions (200 mg L−1) using two varieties of lettuce (Lactuca sativa L.) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L−1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L−1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L−1, but were not able to remove phenol at constant concentration above 25 mg L−1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.",
journal = "Scientia Horticulturae",
title = "Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1",
volume = "231",
doi = "10.1016/j.scienta.2017.12.025",
pages = "210-218"
}

Tadić, V., Petrić, M., Uzelac, B., Milošević, S., Vujčić, Z., Stevanović, J.,& Tadić, J.. (2018). Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1. in Scientia Horticulturae, 231, 210-218.
https://doi.org/10.1016/j.scienta.2017.12.025

Tadić V, Petrić M, Uzelac B, Milošević S, Vujčić Z, Stevanović J, Tadić J. Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1. in Scientia Horticulturae. 2018;231:210-218.
doi:10.1016/j.scienta.2017.12.025 .

Tadić, Vojin, Petrić, Marija, Uzelac, Branka, Milošević, Snežana, Vujčić, Zoran, Stevanović, Jasmina, Tadić, Jovan, "Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1" in Scientia Horticulturae, 231 (2018):210-218,
https://doi.org/10.1016/j.scienta.2017.12.025 . .