TY  - JOUR
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Mirkov, Ivana
AU  - Popović, Dušanka
AU  - Zolotarevski, Lidija
AU  - Despotović, Marta
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5301
AB  - Objective The effect of oral cadmium (Cd) intake to influence contact skin allergies was examined,
since it is known that Cd is a heavy metal that affects many tissues, including the skin, in which it
disturbs homeostasis, thus resulting in inflammation and injury.
Methods Male rats were evoked with experimental contact hypersensitivity reaction (CHS) to hapten
dinitrochlorobenzene (DNCB), after prolonged (30 day) oral exposure to an environmentally relevant Cd
dose (5 ppm). The ear cell population was analyzed with flow cytometry. Cytokine production by ear
skin cells and the activity of skin-draining lymph node (DLN) cells were measured using enzyme-linked
immunosorbent assay (ELISA).
Results Orally acquired Cd (5 ppm) increased CHS intensity only in Dark Agouti (DA) rats by affecting
inflammatory responses in both the sensitization (an increase of IFN-γ and IL-17 cytokine production)
and challenge (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine production)
phases. An increased CHS reaction was seen in Albino Oxford (AO) rats only at a high Cd dose (50 ppm),
during the challenge phase (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine
production).
Conclusion These novel data indicate that oral Cd intensifies the skin response to sensitizing chemicals
such as DNCB.
PB  - Beijing: Chinese Center for Disease Control and Prevention
T2  - Biomedical and Environmental Sciences
T1  - Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats
IS  - 11
VL  - 35
DO  - 10.3967/bes2022.132
SP  - 1038
EP  - 1050
ER  - 

@article{
author = "Tucović, Dina and Kulaš, Jelena and Mirkov, Ivana and Popović, Dušanka and Zolotarevski, Lidija and Despotović, Marta and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2022",
abstract = "Objective The effect of oral cadmium (Cd) intake to influence contact skin allergies was examined,
since it is known that Cd is a heavy metal that affects many tissues, including the skin, in which it
disturbs homeostasis, thus resulting in inflammation and injury.
Methods Male rats were evoked with experimental contact hypersensitivity reaction (CHS) to hapten
dinitrochlorobenzene (DNCB), after prolonged (30 day) oral exposure to an environmentally relevant Cd
dose (5 ppm). The ear cell population was analyzed with flow cytometry. Cytokine production by ear
skin cells and the activity of skin-draining lymph node (DLN) cells were measured using enzyme-linked
immunosorbent assay (ELISA).
Results Orally acquired Cd (5 ppm) increased CHS intensity only in Dark Agouti (DA) rats by affecting
inflammatory responses in both the sensitization (an increase of IFN-γ and IL-17 cytokine production)
and challenge (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine production)
phases. An increased CHS reaction was seen in Albino Oxford (AO) rats only at a high Cd dose (50 ppm),
during the challenge phase (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine
production).
Conclusion These novel data indicate that oral Cd intensifies the skin response to sensitizing chemicals
such as DNCB.",
publisher = "Beijing: Chinese Center for Disease Control and Prevention",
journal = "Biomedical and Environmental Sciences",
title = "Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats",
number = "11",
volume = "35",
doi = "10.3967/bes2022.132",
pages = "1038-1050"
}

Tucović, D., Kulaš, J., Mirkov, I., Popović, D., Zolotarevski, L., Despotović, M., Kataranovski, M.,& Popov Aleksandrov, A.. (2022). Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats. in Biomedical and Environmental Sciences
Beijing: Chinese Center for Disease Control and Prevention., 35(11), 1038-1050.
https://doi.org/10.3967/bes2022.132

Tucović D, Kulaš J, Mirkov I, Popović D, Zolotarevski L, Despotović M, Kataranovski M, Popov Aleksandrov A. Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats. in Biomedical and Environmental Sciences. 2022;35(11):1038-1050.
doi:10.3967/bes2022.132 .

Tucović, Dina, Kulaš, Jelena, Mirkov, Ivana, Popović, Dušanka, Zolotarevski, Lidija, Despotović, Marta, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats" in Biomedical and Environmental Sciences, 35, no. 11 (2022):1038-1050,
https://doi.org/10.3967/bes2022.132 . .

TY  - CONF
AU  - Tucović, Dina
AU  - Mirkov, Ivana
AU  - Kulaš, Jelena
AU  - Popović, Dušanka
AU  - Zolotarevski, Lidija
AU  - Despotović, Marta
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2021
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4882
AB  - Cadmium (Cd) in food and drinking water presents a health risk to the general population. We have shown previously that orally‐acquired Cd affects basal immune homeostasis in the skin. In this study, we examined the effect of 30‐days oral exposure of inflammatory disease‐prone Dark Agouti (DA) rats to two environmentally relevant Cd doses (5 and 50 ppm) on contact hypersensitivity reaction (CHS) induced by topical 0,4% dinitrochlorobenzene (DNCB). Both Cd doses increased proinflammatory epidermal cell response (IL‐1, TNF and IL‐6 production) to DNCB sensitization, as well as epidermal cells’ potential to stimulate naïve lymphocytes ex vivo (increased IFN‐γ and IL‐17 production in co‐cultures). The proinflammatory milieu of epidermal cells induced by sensitization was accompanied by increased hapten‐specific production of IFN‐γ (at a lower Cd dose) and IL‐17 (at both Cd doses) by draining lymph node (DLN) cells, compared to Cd non‐treated animals. During the challenge phase of CHS, oral Cd increased ear swelling response and skin inflammation (edema, mononuclear and neutrophil cell infiltration) at both Cd doses, what correlated with increased innate (TNF) and hapten‐specific effector (IFN‐γ, IL‐17) cytokine response by ear cells. Even in Albino Oxford (AO) rats generally less prone to inflammation, oral Cd increased the proinflammatory response of epidermal cells following sensitization, however, DLN cell responses were absent. Ear swelling response to hapten challenge was observed in AO individuals which acquired a higher Cd dose. Presented data imply the potential of food‐ and water‐borne Cd to be risk factors for skin disease development and/or its exacerbation.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Oral cadmium increases contact hypersensitivity reaction in rats
DO  - 10.1002/eji.202170200
SP  - 348
ER  - 

@conference{
author = "Tucović, Dina and Mirkov, Ivana and Kulaš, Jelena and Popović, Dušanka and Zolotarevski, Lidija and Despotović, Marta and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2021",
abstract = "Cadmium (Cd) in food and drinking water presents a health risk to the general population. We have shown previously that orally‐acquired Cd affects basal immune homeostasis in the skin. In this study, we examined the effect of 30‐days oral exposure of inflammatory disease‐prone Dark Agouti (DA) rats to two environmentally relevant Cd doses (5 and 50 ppm) on contact hypersensitivity reaction (CHS) induced by topical 0,4% dinitrochlorobenzene (DNCB). Both Cd doses increased proinflammatory epidermal cell response (IL‐1, TNF and IL‐6 production) to DNCB sensitization, as well as epidermal cells’ potential to stimulate naïve lymphocytes ex vivo (increased IFN‐γ and IL‐17 production in co‐cultures). The proinflammatory milieu of epidermal cells induced by sensitization was accompanied by increased hapten‐specific production of IFN‐γ (at a lower Cd dose) and IL‐17 (at both Cd doses) by draining lymph node (DLN) cells, compared to Cd non‐treated animals. During the challenge phase of CHS, oral Cd increased ear swelling response and skin inflammation (edema, mononuclear and neutrophil cell infiltration) at both Cd doses, what correlated with increased innate (TNF) and hapten‐specific effector (IFN‐γ, IL‐17) cytokine response by ear cells. Even in Albino Oxford (AO) rats generally less prone to inflammation, oral Cd increased the proinflammatory response of epidermal cells following sensitization, however, DLN cell responses were absent. Ear swelling response to hapten challenge was observed in AO individuals which acquired a higher Cd dose. Presented data imply the potential of food‐ and water‐borne Cd to be risk factors for skin disease development and/or its exacerbation.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Oral cadmium increases contact hypersensitivity reaction in rats",
doi = "10.1002/eji.202170200",
pages = "348"
}

Tucović, D., Mirkov, I., Kulaš, J., Popović, D., Zolotarevski, L., Despotović, M., Kataranovski, M.,& Popov Aleksandrov, A.. (2021). Oral cadmium increases contact hypersensitivity reaction in rats. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 348.
https://doi.org/10.1002/eji.202170200

Tucović D, Mirkov I, Kulaš J, Popović D, Zolotarevski L, Despotović M, Kataranovski M, Popov Aleksandrov A. Oral cadmium increases contact hypersensitivity reaction in rats. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;:348.
doi:10.1002/eji.202170200 .

Tucović, Dina, Mirkov, Ivana, Kulaš, Jelena, Popović, Dušanka, Zolotarevski, Lidija, Despotović, Marta, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Oral cadmium increases contact hypersensitivity reaction in rats" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting (2021):348,
https://doi.org/10.1002/eji.202170200 . .

TY  - JOUR
AU  - Tucović, Dina
AU  - Mirkov, Ivana
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Zolotarevski, Lidija
AU  - Đuđjić, Slađana
AU  - Mutić, Jelena
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2020
UR  - internal-pdf://Tucovic et al. - 2020 - Dermatotoxicity of oral cadmium is strain-dependent and related to differences in skin stress response and infla.pdf
UR  - http://www.ncbi.nlm.nih.gov/pubmed/31924569
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3594
AB  - Adverse effects of non-occupational exposure to cadmium (Cd) are increasingly acknowledged. Since our previous study has showed that orally acquired Cd affects skin, the contribution of genetic background to dermatotoxicity of oral cadmium was examined in two rat strains, Albino Oxford (AO) and Dark Agouti (DA), which differed in response to chemicals. While similar accumulation of Cd in the skin of both strains was noted, the skin response to the metal differed. DA rat individuals mounted antioxidant enzyme defense in the skin already at lower Cd dose, in contrast to AO rats which reacted to higher metal dose solely (and less pronounced), implying higher susceptibility of DA strain to Cd dermatotoxicity. Epidermal cells from both strains developed stress response, but higher intensity of antioxidant response in AO rats implied this strain`s better ability to defend against Cd insult. Cd induced epidermal cells' proinflammatory cytokine response only in DA rats. Increased IL-10 seems responsible for the lack of response in AO rats. Differences in the pattern of skin/epidermal cell responsiveness to cadmium give a new insight into repercussion of genetic variability to dermatotoxicity of orally acquired cadmium, bearing relevance for variations in the link between dietary cadmium and inflammation-based skin pathologies.
T2  - Environmental Toxicology and Pharmacology
T1  - Dermatotoxicity of oral cadmium is strain-dependent and related to differences in skin stress response and inflammatory/immune activity.
VL  - 75
DO  - 10.1016/j.etap.2020.103326
SP  - 103326
ER  - 

@article{
author = "Tucović, Dina and Mirkov, Ivana and Kulaš, Jelena and Zeljković, Milica and Popović, Dušanka and Zolotarevski, Lidija and Đuđjić, Slađana and Mutić, Jelena and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2020",
abstract = "Adverse effects of non-occupational exposure to cadmium (Cd) are increasingly acknowledged. Since our previous study has showed that orally acquired Cd affects skin, the contribution of genetic background to dermatotoxicity of oral cadmium was examined in two rat strains, Albino Oxford (AO) and Dark Agouti (DA), which differed in response to chemicals. While similar accumulation of Cd in the skin of both strains was noted, the skin response to the metal differed. DA rat individuals mounted antioxidant enzyme defense in the skin already at lower Cd dose, in contrast to AO rats which reacted to higher metal dose solely (and less pronounced), implying higher susceptibility of DA strain to Cd dermatotoxicity. Epidermal cells from both strains developed stress response, but higher intensity of antioxidant response in AO rats implied this strain`s better ability to defend against Cd insult. Cd induced epidermal cells' proinflammatory cytokine response only in DA rats. Increased IL-10 seems responsible for the lack of response in AO rats. Differences in the pattern of skin/epidermal cell responsiveness to cadmium give a new insight into repercussion of genetic variability to dermatotoxicity of orally acquired cadmium, bearing relevance for variations in the link between dietary cadmium and inflammation-based skin pathologies.",
journal = "Environmental Toxicology and Pharmacology",
title = "Dermatotoxicity of oral cadmium is strain-dependent and related to differences in skin stress response and inflammatory/immune activity.",
volume = "75",
doi = "10.1016/j.etap.2020.103326",
pages = "103326"
}

Tucović, D., Mirkov, I., Kulaš, J., Zeljković, M., Popović, D., Zolotarevski, L., Đuđjić, S., Mutić, J., Kataranovski, M.,& Popov Aleksandrov, A.. (2020). Dermatotoxicity of oral cadmium is strain-dependent and related to differences in skin stress response and inflammatory/immune activity.. in Environmental Toxicology and Pharmacology, 75, 103326.
https://doi.org/10.1016/j.etap.2020.103326

Tucović D, Mirkov I, Kulaš J, Zeljković M, Popović D, Zolotarevski L, Đuđjić S, Mutić J, Kataranovski M, Popov Aleksandrov A. Dermatotoxicity of oral cadmium is strain-dependent and related to differences in skin stress response and inflammatory/immune activity.. in Environmental Toxicology and Pharmacology. 2020;75:103326.
doi:10.1016/j.etap.2020.103326 .

Tucović, Dina, Mirkov, Ivana, Kulaš, Jelena, Zeljković, Milica, Popović, Dušanka, Zolotarevski, Lidija, Đuđjić, Slađana, Mutić, Jelena, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Dermatotoxicity of oral cadmium is strain-dependent and related to differences in skin stress response and inflammatory/immune activity." in Environmental Toxicology and Pharmacology, 75 (2020):103326,
https://doi.org/10.1016/j.etap.2020.103326 . .

TY  - CONF
AU  - Tucović, Dina
AU  - Mirkov, Ivana
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Zolotarevski, Lidija
AU  - Đurđić, Slađana
AU  - Mutić, Jelena
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4795
AB  - Adverse effects of non-occupational exposure to cadmium (Cd) are increasingly
acknowledged. Using a rat model of oral Cd exposure in drinking water we have shown
that skin is a target for this metal. Due to contribution of individual variability to the
intensity of cadmium toxicity, dermatotoxicity of two environmentally relevant Cd
doses (5 and 50 ppm) was examined in individuals of two rat strains, Albino Oxford
(AO) and Dark Agouti (DA), which differ in response to chemicals. A dose-dependent
accumulation of Cd in the skin/epidermal cells was noted in both strains, and although
there were no strain differences in the Cd accumulation, the degree of skin response to
the metal differed. Signs of skin damage were evident in both strains, but response to
injury was more pronounced in DA. Individuals of DA rats responded by an increase
in the levels of antioxidant defense enzymes in the skin already at lower dose, in
contrast to AO (which reacted to higher dose solely), implying higher sensitivity of DA
strain to Cd-induced toxicity. Epidermal cells from both strains developed stress
response, however increased GSH, and higher metallothionein/MT-1 and MT-2
mRNA, Nrf2 protein, apoptosis, Ahr and Cyp genes in AO, depicting this strain`s
ability to better defend against Cd insult. Epidermal cells` IL-1β, TNF and IL-6
response was induced by Cd in DA, while pro-inflammatory cytokine production was
unchanged in AO (though increased following stimulation with S. epidermidis), with
increased IL-10 as a possible underlying mechanism. T cells from non-exposed rats
produce more IFN-γ and IL-17 in co-culture with epidermal cell from Cd–exposed DA
rats what strengthens the view that this strain is more prone to metal’s dermatotoxicity.
These data give a new insight into repercussion of genetic variability to toxicity of
cadmium acquired by the skin via gut, bearing relevance for variations in the link
between dietary cadmium and inflammation-based skin pathologies.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book
T1  - Environmentally relevant exposure to cadmium and health risks: skin as target organ
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4795
ER  - 

@conference{
author = "Tucović, Dina and Mirkov, Ivana and Kulaš, Jelena and Zeljković, Milica and Popović, Dušanka and Zolotarevski, Lidija and Đurđić, Slađana and Mutić, Jelena and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2019",
abstract = "Adverse effects of non-occupational exposure to cadmium (Cd) are increasingly
acknowledged. Using a rat model of oral Cd exposure in drinking water we have shown
that skin is a target for this metal. Due to contribution of individual variability to the
intensity of cadmium toxicity, dermatotoxicity of two environmentally relevant Cd
doses (5 and 50 ppm) was examined in individuals of two rat strains, Albino Oxford
(AO) and Dark Agouti (DA), which differ in response to chemicals. A dose-dependent
accumulation of Cd in the skin/epidermal cells was noted in both strains, and although
there were no strain differences in the Cd accumulation, the degree of skin response to
the metal differed. Signs of skin damage were evident in both strains, but response to
injury was more pronounced in DA. Individuals of DA rats responded by an increase
in the levels of antioxidant defense enzymes in the skin already at lower dose, in
contrast to AO (which reacted to higher dose solely), implying higher sensitivity of DA
strain to Cd-induced toxicity. Epidermal cells from both strains developed stress
response, however increased GSH, and higher metallothionein/MT-1 and MT-2
mRNA, Nrf2 protein, apoptosis, Ahr and Cyp genes in AO, depicting this strain`s
ability to better defend against Cd insult. Epidermal cells` IL-1β, TNF and IL-6
response was induced by Cd in DA, while pro-inflammatory cytokine production was
unchanged in AO (though increased following stimulation with S. epidermidis), with
increased IL-10 as a possible underlying mechanism. T cells from non-exposed rats
produce more IFN-γ and IL-17 in co-culture with epidermal cell from Cd–exposed DA
rats what strengthens the view that this strain is more prone to metal’s dermatotoxicity.
These data give a new insight into repercussion of genetic variability to toxicity of
cadmium acquired by the skin via gut, bearing relevance for variations in the link
between dietary cadmium and inflammation-based skin pathologies.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book",
title = "Environmentally relevant exposure to cadmium and health risks: skin as target organ",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4795"
}

Tucović, D., Mirkov, I., Kulaš, J., Zeljković, M., Popović, D., Zolotarevski, L., Đurđić, S., Mutić, J., Kataranovski, M.,& Popov Aleksandrov, A.. (2019). Environmentally relevant exposure to cadmium and health risks: skin as target organ. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 79.
https://hdl.handle.net/21.15107/rcub_ibiss_4795

Tucović D, Mirkov I, Kulaš J, Zeljković M, Popović D, Zolotarevski L, Đurđić S, Mutić J, Kataranovski M, Popov Aleksandrov A. Environmentally relevant exposure to cadmium and health risks: skin as target organ. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book. 2019;:79.
https://hdl.handle.net/21.15107/rcub_ibiss_4795 .

Tucović, Dina, Mirkov, Ivana, Kulaš, Jelena, Zeljković, Milica, Popović, Dušanka, Zolotarevski, Lidija, Đurđić, Slađana, Mutić, Jelena, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Environmentally relevant exposure to cadmium and health risks: skin as target organ" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book (2019):79,
https://hdl.handle.net/21.15107/rcub_ibiss_4795 .

TY  - JOUR
AU  - Kulaš, Jelena
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Zolotarevski, Lidija
AU  - Glamočlija, Jasmina
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Golić, Nataša
AU  - Kataranovski, Milena
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0171298518301001?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3165
AB  - Microbiota inhabiting mucosal tissues is involved in maintenance of their immune homeostasis. Growing body of evidence indicate that dysbiosis in gut influence immune responses at distal sites including lungs. There are also reports concerning gut involvement with pulmonary injury/inflammation in settings of respiratory viral and bacterial infections. The impact of infections with other microorganisms on gut homeostasis is not explored. In this study, the rat model of sublethal pulmonary infection with Aspergillus fumigatus was used to investigate the effect of fungal respiratory infection on gut immune-mediated homeostasis. Signs of intestinal damage, intestinal and gut-draining lymphoid tissue cytokine responses and gut bacterial microbiota diversity were examined. Intestinal injury, inflammatory cell infiltration, as well as increased levels of intestinal interferon-γ (IFN-γ) and interleukin-17 (IL-17) (as opposed to unchanged levels of anti-inflammatory cytokine IL-10) during the two-week period depict intestinal inflammation in rats with pulmonary A. fumigatus infection. It could not be ascribed to the fungus as it was not detected in the intestine of infected rats. Increased production of pro-inflammatory cytokines by major gut-draining mesenteric lymph nodes point to these lymphoid organs as places of generation of cytokine-producing cells. No changes in spleen or systemic cytokine responses was observed, showing lack of the effects of pulmonary A. fumigatus infection outside mucosal immune system. Drop of intestinal bacterial microbiota diversity (disappearance of several bacterial bands) was noted early in infection with normalization starting from day seven. From day three, appearance of new bacterial bands (unique to infected individuals, not present in controls) was seen, and some of them are pathogens. Alterations in intestinal bacterial community might have affected intestinal immune tolerance contributing to inflammation. Disruption of gut homeostasis during pulmonary infection might render gastrointestinal tract more susceptible to variety of physiological and pathological stimuli. Data which showed for the first time gut involvement with pulmonary infection with A. fumigatus provide the baseline for future studies of the impact of fungal lung infections to gut homeostasis, particularly in individuals susceptible to these infections.
T2  - Immunobiology
T2  - Immunobiology
T1  - Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis.
IS  - 1
VL  - 224
DO  - 10.1016/j.imbio.2018.10.001
SP  - 116
EP  - 123
ER  - 

@article{
author = "Kulaš, Jelena and Mirkov, Ivana and Tucović, Dina and Zolotarevski, Lidija and Glamočlija, Jasmina and Veljović, Katarina and Tolinački, Maja and Golić, Nataša and Kataranovski, Milena",
year = "2019",
abstract = "Microbiota inhabiting mucosal tissues is involved in maintenance of their immune homeostasis. Growing body of evidence indicate that dysbiosis in gut influence immune responses at distal sites including lungs. There are also reports concerning gut involvement with pulmonary injury/inflammation in settings of respiratory viral and bacterial infections. The impact of infections with other microorganisms on gut homeostasis is not explored. In this study, the rat model of sublethal pulmonary infection with Aspergillus fumigatus was used to investigate the effect of fungal respiratory infection on gut immune-mediated homeostasis. Signs of intestinal damage, intestinal and gut-draining lymphoid tissue cytokine responses and gut bacterial microbiota diversity were examined. Intestinal injury, inflammatory cell infiltration, as well as increased levels of intestinal interferon-γ (IFN-γ) and interleukin-17 (IL-17) (as opposed to unchanged levels of anti-inflammatory cytokine IL-10) during the two-week period depict intestinal inflammation in rats with pulmonary A. fumigatus infection. It could not be ascribed to the fungus as it was not detected in the intestine of infected rats. Increased production of pro-inflammatory cytokines by major gut-draining mesenteric lymph nodes point to these lymphoid organs as places of generation of cytokine-producing cells. No changes in spleen or systemic cytokine responses was observed, showing lack of the effects of pulmonary A. fumigatus infection outside mucosal immune system. Drop of intestinal bacterial microbiota diversity (disappearance of several bacterial bands) was noted early in infection with normalization starting from day seven. From day three, appearance of new bacterial bands (unique to infected individuals, not present in controls) was seen, and some of them are pathogens. Alterations in intestinal bacterial community might have affected intestinal immune tolerance contributing to inflammation. Disruption of gut homeostasis during pulmonary infection might render gastrointestinal tract more susceptible to variety of physiological and pathological stimuli. Data which showed for the first time gut involvement with pulmonary infection with A. fumigatus provide the baseline for future studies of the impact of fungal lung infections to gut homeostasis, particularly in individuals susceptible to these infections.",
journal = "Immunobiology, Immunobiology",
title = "Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis.",
number = "1",
volume = "224",
doi = "10.1016/j.imbio.2018.10.001",
pages = "116-123"
}

Kulaš, J., Mirkov, I., Tucović, D., Zolotarevski, L., Glamočlija, J., Veljović, K., Tolinački, M., Golić, N.,& Kataranovski, M.. (2019). Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis.. in Immunobiology, 224(1), 116-123.
https://doi.org/10.1016/j.imbio.2018.10.001

Kulaš J, Mirkov I, Tucović D, Zolotarevski L, Glamočlija J, Veljović K, Tolinački M, Golić N, Kataranovski M. Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis.. in Immunobiology. 2019;224(1):116-123.
doi:10.1016/j.imbio.2018.10.001 .

Kulaš, Jelena, Mirkov, Ivana, Tucović, Dina, Zolotarevski, Lidija, Glamočlija, Jasmina, Veljović, Katarina, Tolinački, Maja, Golić, Nataša, Kataranovski, Milena, "Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis." in Immunobiology, 224, no. 1 (2019):116-123,
https://doi.org/10.1016/j.imbio.2018.10.001 . .

TY  - JOUR
AU  - Tucović, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Kulaš, Jelena
AU  - Zolotarevski, Lidija
AU  - Vukojević, Vesna
AU  - Mutić, Jelena
AU  - Tatalović, Nikola
AU  - Kataranovski, Milena
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0147651318307231?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3126
AB  - Skin can acquire cadmium (Cd) by oral route, but there is paucity of data concerning cutaneous effects of this metal. Cd acquired by oral route can affect skin wound healing, but the effect of Cd on other activities involved in skin homeostasis, including skin immunity, are not explored. Using the rat model of 30-day oral administration of Cd (5 ppm and 50 ppm) in drinking water, basic aspects of immune-relevant activity of epidermal cells were examined. Dose-dependent Cd deposition in the the skin was observed (0.035 ± 0.02 µg/g and 0.127 ± 0.04 µg/g at 5 ppm and 50 ppm, respectively, compared to 0.012 ± 0.009 µg/g at 0 ppm of Cd). This resulted in skin inflammation (oxidative stress at both Cd doses and dose-dependent structural changes in the skin and the presence/activation of innate immunity cells). At low Cd dose inflammatory response (nitric oxide and IL-1β) was observed. Other inflammatory cytokines (IL-6 and TNF) response occurred at 50 ppm, which was increased further following skin sensitization with contact allergen dinitro-chlorobenzene (DNCB). Epidermal cells exposed to both Cd doses enhanced concanavalin A (ConA)-stimulated lymphocyte production of IL-17. This study showed for the first time the effect of the metal which gained access to the skin via gut on immune reactivity of epidermal cells. Presented data might be relevant for the link between dietary Cd and the risk of skin pathologies.
T2  - Ecotoxicology and Environmental Safety
T1  - Oral cadmium exposure affects skin immune reactivity in rats.
VL  - 164
DO  - 10.1016/j.ecoenv.2018.07.117
SP  - 12
EP  - 20
ER  - 

@article{
author = "Tucović, Dina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Ninkov, Marina and Kulaš, Jelena and Zolotarevski, Lidija and Vukojević, Vesna and Mutić, Jelena and Tatalović, Nikola and Kataranovski, Milena",
year = "2018",
abstract = "Skin can acquire cadmium (Cd) by oral route, but there is paucity of data concerning cutaneous effects of this metal. Cd acquired by oral route can affect skin wound healing, but the effect of Cd on other activities involved in skin homeostasis, including skin immunity, are not explored. Using the rat model of 30-day oral administration of Cd (5 ppm and 50 ppm) in drinking water, basic aspects of immune-relevant activity of epidermal cells were examined. Dose-dependent Cd deposition in the the skin was observed (0.035 ± 0.02 µg/g and 0.127 ± 0.04 µg/g at 5 ppm and 50 ppm, respectively, compared to 0.012 ± 0.009 µg/g at 0 ppm of Cd). This resulted in skin inflammation (oxidative stress at both Cd doses and dose-dependent structural changes in the skin and the presence/activation of innate immunity cells). At low Cd dose inflammatory response (nitric oxide and IL-1β) was observed. Other inflammatory cytokines (IL-6 and TNF) response occurred at 50 ppm, which was increased further following skin sensitization with contact allergen dinitro-chlorobenzene (DNCB). Epidermal cells exposed to both Cd doses enhanced concanavalin A (ConA)-stimulated lymphocyte production of IL-17. This study showed for the first time the effect of the metal which gained access to the skin via gut on immune reactivity of epidermal cells. Presented data might be relevant for the link between dietary Cd and the risk of skin pathologies.",
journal = "Ecotoxicology and Environmental Safety",
title = "Oral cadmium exposure affects skin immune reactivity in rats.",
volume = "164",
doi = "10.1016/j.ecoenv.2018.07.117",
pages = "12-20"
}

Tucović, D., Popov Aleksandrov, A., Mirkov, I., Ninkov, M., Kulaš, J., Zolotarevski, L., Vukojević, V., Mutić, J., Tatalović, N.,& Kataranovski, M.. (2018). Oral cadmium exposure affects skin immune reactivity in rats.. in Ecotoxicology and Environmental Safety, 164, 12-20.
https://doi.org/10.1016/j.ecoenv.2018.07.117

Tucović D, Popov Aleksandrov A, Mirkov I, Ninkov M, Kulaš J, Zolotarevski L, Vukojević V, Mutić J, Tatalović N, Kataranovski M. Oral cadmium exposure affects skin immune reactivity in rats.. in Ecotoxicology and Environmental Safety. 2018;164:12-20.
doi:10.1016/j.ecoenv.2018.07.117 .

Tucović, Dina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Ninkov, Marina, Kulaš, Jelena, Zolotarevski, Lidija, Vukojević, Vesna, Mutić, Jelena, Tatalović, Nikola, Kataranovski, Milena, "Oral cadmium exposure affects skin immune reactivity in rats." in Ecotoxicology and Environmental Safety, 164 (2018):12-20,
https://doi.org/10.1016/j.ecoenv.2018.07.117 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Zolotarevski, Lidija
AU  - Ninkov, Marina
AU  - Tucović, Dina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2017
UR  - https://www.sciencedirect.com/science/article/pii/S1382668917301746?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3540
AB  - Warfarin is an anticoagulant used in prevention/prophylaxis of thromboembolism. Besides the effects on coagulation, non-hemorrhagic reactions have also been documented. Although cutaneous reactions were reported in some patients, the impact on skin immunity was not explored. In the present paper, the effect of 30-day oral warfarin intake on skin cytokine responses in rats was analyzed. Increased release of inflammatory cytokines (TNF, IL-1β and IL-10) was noted by skin explants from rats which received warfarin, but without effect on IL-6. No impact on epidermal cell cytokine secretion was seen, except a tendency of an increase of IL-6 response to stimulation with microbial product lipopolysaccharide (LPS). Topical application of contact allergen dinitrochlorobenzene (DNCB) resulted in slight (numerical solely) increase of TNF release by skin explants of warfarin-treated animals, while epidermal cells responded by increased secretion of all four cytokines examined. The data presented provide new information on the potential of oral warfarin to modulate skin innate immune activity.
T2  - Environmental Toxicology and Pharmacology
T1  - Oral warfarin intake affects skin inflammatory cytokine responses in rats.
VL  - 54
DO  - 10.1016/j.etap.2017.06.027
SP  - 93
EP  - 98
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Zolotarevski, Lidija and Ninkov, Marina and Tucović, Dina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2017",
abstract = "Warfarin is an anticoagulant used in prevention/prophylaxis of thromboembolism. Besides the effects on coagulation, non-hemorrhagic reactions have also been documented. Although cutaneous reactions were reported in some patients, the impact on skin immunity was not explored. In the present paper, the effect of 30-day oral warfarin intake on skin cytokine responses in rats was analyzed. Increased release of inflammatory cytokines (TNF, IL-1β and IL-10) was noted by skin explants from rats which received warfarin, but without effect on IL-6. No impact on epidermal cell cytokine secretion was seen, except a tendency of an increase of IL-6 response to stimulation with microbial product lipopolysaccharide (LPS). Topical application of contact allergen dinitrochlorobenzene (DNCB) resulted in slight (numerical solely) increase of TNF release by skin explants of warfarin-treated animals, while epidermal cells responded by increased secretion of all four cytokines examined. The data presented provide new information on the potential of oral warfarin to modulate skin innate immune activity.",
journal = "Environmental Toxicology and Pharmacology",
title = "Oral warfarin intake affects skin inflammatory cytokine responses in rats.",
volume = "54",
doi = "10.1016/j.etap.2017.06.027",
pages = "93-98"
}

Popov Aleksandrov, A., Mirkov, I., Zolotarevski, L., Ninkov, M., Tucović, D., Kataranovski, D.,& Kataranovski, M.. (2017). Oral warfarin intake affects skin inflammatory cytokine responses in rats.. in Environmental Toxicology and Pharmacology, 54, 93-98.
https://doi.org/10.1016/j.etap.2017.06.027

Popov Aleksandrov A, Mirkov I, Zolotarevski L, Ninkov M, Tucović D, Kataranovski D, Kataranovski M. Oral warfarin intake affects skin inflammatory cytokine responses in rats.. in Environmental Toxicology and Pharmacology. 2017;54:93-98.
doi:10.1016/j.etap.2017.06.027 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Zolotarevski, Lidija, Ninkov, Marina, Tucović, Dina, Kataranovski, Dragan, Kataranovski, Milena, "Oral warfarin intake affects skin inflammatory cytokine responses in rats." in Environmental Toxicology and Pharmacology, 54 (2017):93-98,
https://doi.org/10.1016/j.etap.2017.06.027 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Demenesku, Jelena
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Stefik, Debora
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4813
AB  - Intestinal hemorrhage characterizes effectiveness of warfarin (WF) as rodenticide and is amongadverse effects of therapy in humans. Having in mind genetic variations in the effectiveness ofWF in wild rats and in the doses required for therapeutic effect, strain differences in the intestinaltoxicity of oral warfarin in rats were examined in this study. High WF dose (3.5 mg/l) led tomortality in Albino Oxford (AO) rats, with no lethality in Dark Agouti (DA) rats. Higher valuesof prothrombin time were noted at low WF dose (0.35 mg/l) in the former strain. Leukocyteinfiltration in intestine noted at this dose in both strains was associated with oxidative injury andmore pronounced anti-oxidative response in AO rats. Suppression of mesenteric lymph node cellproliferation and IFN-γ and IL-10 production in AO rats and lack of these effects in DA rats,represent different strategies to protect vulnerable intestine from harmful immune responses.
PB  - Amsterdam, Holland:Elsevier B.V.
T2  - Environmental Toxicology and Pharmacology
T1  - Strain differences in intestinal toxicity of warfarin in rats
VL  - 48
DO  - 10.1016/j.etap.2016.10.019
SP  - 175
EP  - 182
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Mileusnić, Dina and Demenesku, Jelena and Zolotarevski, Lidija and Subota, Vesna and Stefik, Debora and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Intestinal hemorrhage characterizes effectiveness of warfarin (WF) as rodenticide and is amongadverse effects of therapy in humans. Having in mind genetic variations in the effectiveness ofWF in wild rats and in the doses required for therapeutic effect, strain differences in the intestinaltoxicity of oral warfarin in rats were examined in this study. High WF dose (3.5 mg/l) led tomortality in Albino Oxford (AO) rats, with no lethality in Dark Agouti (DA) rats. Higher valuesof prothrombin time were noted at low WF dose (0.35 mg/l) in the former strain. Leukocyteinfiltration in intestine noted at this dose in both strains was associated with oxidative injury andmore pronounced anti-oxidative response in AO rats. Suppression of mesenteric lymph node cellproliferation and IFN-γ and IL-10 production in AO rats and lack of these effects in DA rats,represent different strategies to protect vulnerable intestine from harmful immune responses.",
publisher = "Amsterdam, Holland:Elsevier B.V.",
journal = "Environmental Toxicology and Pharmacology",
title = "Strain differences in intestinal toxicity of warfarin in rats",
volume = "48",
doi = "10.1016/j.etap.2016.10.019",
pages = "175-182"
}

Mirkov, I., Popov Aleksandrov, A., Ninkov, M., Mileusnić, D., Demenesku, J., Zolotarevski, L., Subota, V., Stefik, D., Kataranovski, D.,& Kataranovski, M.. (2016). Strain differences in intestinal toxicity of warfarin in rats. in Environmental Toxicology and Pharmacology
Amsterdam, Holland:Elsevier B.V.., 48, 175-182.
https://doi.org/10.1016/j.etap.2016.10.019

Mirkov I, Popov Aleksandrov A, Ninkov M, Mileusnić D, Demenesku J, Zolotarevski L, Subota V, Stefik D, Kataranovski D, Kataranovski M. Strain differences in intestinal toxicity of warfarin in rats. in Environmental Toxicology and Pharmacology. 2016;48:175-182.
doi:10.1016/j.etap.2016.10.019 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Mileusnić, Dina, Demenesku, Jelena, Zolotarevski, Lidija, Subota, Vesna, Stefik, Debora, Kataranovski, Dragan, Kataranovski, Milena, "Strain differences in intestinal toxicity of warfarin in rats" in Environmental Toxicology and Pharmacology, 48 (2016):175-182,
https://doi.org/10.1016/j.etap.2016.10.019 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Demenesku, Jelena
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Stefik, Debora
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4812
AB  - Intestinal hemorrhage characterizes effectiveness of warfarin (WF) as rodenticide and is among
adverse effects of therapy in humans. Having in mind genetic variations in the effectiveness of
WF in wild rats and in the doses required for therapeutic effect, strain differences in the intestinal
toxicity of oral warfarin in rats were examined in this study. High WF dose (3.5 mg/l) led to
mortality in Albino Oxford (AO) rats, with no lethality in Dark Agouti (DA) rats. Higher values
of prothrombin time were noted at low WF dose (0.35 mg/l) in the former strain. Leukocyte
infiltration in intestine noted at this dose in both strains was associated with oxidative injury and
more pronounced anti-oxidative response in AO rats. Suppression of mesenteric lymph node cell
proliferation and IFN-γ and IL-10 production in AO rats and lack of these effects in DA rats,
represent different strategies to protect vulnerable intestine from harmful immune responses.
PB  - Amsterdam, Holland:Elsevier B.V.
T2  - Environmental Toxicology and Pharmacology
T1  - Strain differences in intestinal toxicity of warfarin in rats
VL  - 48
DO  - 10.1016/j.etap.2016.10.019
SP  - 175
EP  - 182
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Mileusnić, Dina and Demenesku, Jelena and Zolotarevski, Lidija and Subota, Vesna and Stefik, Debora and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Intestinal hemorrhage characterizes effectiveness of warfarin (WF) as rodenticide and is among
adverse effects of therapy in humans. Having in mind genetic variations in the effectiveness of
WF in wild rats and in the doses required for therapeutic effect, strain differences in the intestinal
toxicity of oral warfarin in rats were examined in this study. High WF dose (3.5 mg/l) led to
mortality in Albino Oxford (AO) rats, with no lethality in Dark Agouti (DA) rats. Higher values
of prothrombin time were noted at low WF dose (0.35 mg/l) in the former strain. Leukocyte
infiltration in intestine noted at this dose in both strains was associated with oxidative injury and
more pronounced anti-oxidative response in AO rats. Suppression of mesenteric lymph node cell
proliferation and IFN-γ and IL-10 production in AO rats and lack of these effects in DA rats,
represent different strategies to protect vulnerable intestine from harmful immune responses.",
publisher = "Amsterdam, Holland:Elsevier B.V.",
journal = "Environmental Toxicology and Pharmacology",
title = "Strain differences in intestinal toxicity of warfarin in rats",
volume = "48",
doi = "10.1016/j.etap.2016.10.019",
pages = "175-182"
}

Mirkov, I., Popov Aleksandrov, A., Ninkov, M., Mileusnić, D., Demenesku, J., Zolotarevski, L., Subota, V., Stefik, D., Kataranovski, D.,& Kataranovski, M.. (2016). Strain differences in intestinal toxicity of warfarin in rats. in Environmental Toxicology and Pharmacology
Amsterdam, Holland:Elsevier B.V.., 48, 175-182.
https://doi.org/10.1016/j.etap.2016.10.019

Mirkov I, Popov Aleksandrov A, Ninkov M, Mileusnić D, Demenesku J, Zolotarevski L, Subota V, Stefik D, Kataranovski D, Kataranovski M. Strain differences in intestinal toxicity of warfarin in rats. in Environmental Toxicology and Pharmacology. 2016;48:175-182.
doi:10.1016/j.etap.2016.10.019 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Mileusnić, Dina, Demenesku, Jelena, Zolotarevski, Lidija, Subota, Vesna, Stefik, Debora, Kataranovski, Dragan, Kataranovski, Milena, "Strain differences in intestinal toxicity of warfarin in rats" in Environmental Toxicology and Pharmacology, 48 (2016):175-182,
https://doi.org/10.1016/j.etap.2016.10.019 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4815
AB  - Though warfarin is extensively used in the prevention and treatment of thromboembolic
processes in humans, adverse effects of warfarin therapy have been recognized. Intestinal
hemorrhage is one of the hazards of anticoagulant therapy, but the mechanisms of warfarin
toxicity are virtually unknown. In this work, the effects of 30 days oral warfarin (0.35 mg/l and
3.5 mg/l) intake on rat’s gut were examined. Both doses resulted in prolongation of prothrombin
time. Systemic effects of higher warfarin dose (increases in plasma AST, proteinuria, hematuria,
changes in peripheral blood hematological parameters) were seen. Warfarin intake resulted in
histologically evident tissue damage, leukocyte infiltration and intestinal inflammation [increases
in myeloperoxidase activity, malondialdehyde content, superoxide dismutase and catalase
activity, proinflammatory cytokine (IFN-γ, IL-17) concentrations in intestinal homogenates]. In
contrast, suppression of gut-draining mesenteric lymph node (MLN) cell activity [proliferation
responsiveness, production of IFN-γ and IL-17 to T lymphocyte mitogen Concanavalin A
stimulation] was noted. Inhibition of regulatory cytokine IL-10 production by MLN cells,
suggests commitment of MLN to the suppression of all inflammatory activities and creation of
the microenvironment which is non-permissive for induction of potentially harmful immune
response. These novel findings indicate the need of staying alert for (adverse) effects of warfarin
therapy.
PB  - Amsterdam : Elsevier
T2  - Food and Chemical Toxicology
T1  - Intestinal toxicity of oral warfarin intake in rats
VL  - 94
DO  - 10.1016/j.fct.2016.05.007
SP  - 11
EP  - 18
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Ninkov, Marina and Mileusnić, Dina and Zolotarevski, Lidija and Subota, Vesna and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Though warfarin is extensively used in the prevention and treatment of thromboembolic
processes in humans, adverse effects of warfarin therapy have been recognized. Intestinal
hemorrhage is one of the hazards of anticoagulant therapy, but the mechanisms of warfarin
toxicity are virtually unknown. In this work, the effects of 30 days oral warfarin (0.35 mg/l and
3.5 mg/l) intake on rat’s gut were examined. Both doses resulted in prolongation of prothrombin
time. Systemic effects of higher warfarin dose (increases in plasma AST, proteinuria, hematuria,
changes in peripheral blood hematological parameters) were seen. Warfarin intake resulted in
histologically evident tissue damage, leukocyte infiltration and intestinal inflammation [increases
in myeloperoxidase activity, malondialdehyde content, superoxide dismutase and catalase
activity, proinflammatory cytokine (IFN-γ, IL-17) concentrations in intestinal homogenates]. In
contrast, suppression of gut-draining mesenteric lymph node (MLN) cell activity [proliferation
responsiveness, production of IFN-γ and IL-17 to T lymphocyte mitogen Concanavalin A
stimulation] was noted. Inhibition of regulatory cytokine IL-10 production by MLN cells,
suggests commitment of MLN to the suppression of all inflammatory activities and creation of
the microenvironment which is non-permissive for induction of potentially harmful immune
response. These novel findings indicate the need of staying alert for (adverse) effects of warfarin
therapy.",
publisher = "Amsterdam : Elsevier",
journal = "Food and Chemical Toxicology",
title = "Intestinal toxicity of oral warfarin intake in rats",
volume = "94",
doi = "10.1016/j.fct.2016.05.007",
pages = "11-18"
}

Mirkov, I., Popov Aleksandrov, A., Demenesku, J., Ninkov, M., Mileusnić, D., Zolotarevski, L., Subota, V., Kataranovski, D.,& Kataranovski, M.. (2016). Intestinal toxicity of oral warfarin intake in rats. in Food and Chemical Toxicology
Amsterdam : Elsevier., 94, 11-18.
https://doi.org/10.1016/j.fct.2016.05.007

Mirkov I, Popov Aleksandrov A, Demenesku J, Ninkov M, Mileusnić D, Zolotarevski L, Subota V, Kataranovski D, Kataranovski M. Intestinal toxicity of oral warfarin intake in rats. in Food and Chemical Toxicology. 2016;94:11-18.
doi:10.1016/j.fct.2016.05.007 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Ninkov, Marina, Mileusnić, Dina, Zolotarevski, Lidija, Subota, Vesna, Kataranovski, Dragan, Kataranovski, Milena, "Intestinal toxicity of oral warfarin intake in rats" in Food and Chemical Toxicology, 94 (2016):11-18,
https://doi.org/10.1016/j.fct.2016.05.007 . .

TY  - CONF
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4853
AB  - Warfarin is an anticoagulant that is widely used in the prevention and treatment of thromboembolic disorders in humans. Numerous side effects of oral therapy with this agent are known, which has led to the recommendation for transdermal administration of this agent. This study examined the effect of oral warfarin consumption (0.35 mg / l and 3.5 mg / l in drinking water, 30 days) on the intestinal immune system in rats, as well as the systemic effect (on peripheral blood leukocytes) of epicutaneous administration of this agent. µg or 100 µg, once a day, for three days).The anticoagulant effect, determined on the basis of the increase in prothrombin time, was observed after oral consumption of both doses, as well as after epicutaneous application. Orally administered warfarin leads to histologically evident damage to intestinal tissue and inflammation (cellular infiltration, myeloperoxidase activity, malodialdehyde content and superoxide dismutase and catalase activities), as well as increased concentrations of proinflammatory cytokines (IFN-γ, IL-17) in intestinal homogenate.In mesenteric lymph nodes, however, suppression of the immune response has been observed (decreased ability of cells to proliferate and produce IFN-γ and IL-17 in response to cannavalin A stimulation). Decreased production of IL-10 by mesenteric lymph node cells indicates the formation of a microenvironment that does not allow the activation of a potentially harmful immune response in this tissue. Epicutaneous administration of a higher dose of warfarin leads to an increase in the number of neutrophilic leukocytes and intracellular myeloperoxidase activity, as well as an increase in granular CD11b + cells. In contrast to this increase, a decrease in TNF and IL-6 production as well as mRNA levels for these cytokines was observed.After administration of a higher dose of warfarin, there is a decrease in the number of mononuclear cells with an increase in the presence of CD11b + in this population, but without an effect on cytokine production, indicating the differential effects of transdermal administration of warfarin. Taken together, the results indicate the need to monitor the (adverse) effects of warfarin therapy.
PB  - Belgrade: Immunological Society of Serbia
C3  - VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata
T1  - Immunomodulating effect of oral and transdermal varfarine therapy
T1  - Imunomodulatorni efekti oralne i transdermalne terapije varfarinom
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4853
ER  - 

@conference{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Ninkov, Marina and Mileusnić, Dina and Zolotarevski, Lidija and Subota, Vesna and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Warfarin is an anticoagulant that is widely used in the prevention and treatment of thromboembolic disorders in humans. Numerous side effects of oral therapy with this agent are known, which has led to the recommendation for transdermal administration of this agent. This study examined the effect of oral warfarin consumption (0.35 mg / l and 3.5 mg / l in drinking water, 30 days) on the intestinal immune system in rats, as well as the systemic effect (on peripheral blood leukocytes) of epicutaneous administration of this agent. µg or 100 µg, once a day, for three days).The anticoagulant effect, determined on the basis of the increase in prothrombin time, was observed after oral consumption of both doses, as well as after epicutaneous application. Orally administered warfarin leads to histologically evident damage to intestinal tissue and inflammation (cellular infiltration, myeloperoxidase activity, malodialdehyde content and superoxide dismutase and catalase activities), as well as increased concentrations of proinflammatory cytokines (IFN-γ, IL-17) in intestinal homogenate.In mesenteric lymph nodes, however, suppression of the immune response has been observed (decreased ability of cells to proliferate and produce IFN-γ and IL-17 in response to cannavalin A stimulation). Decreased production of IL-10 by mesenteric lymph node cells indicates the formation of a microenvironment that does not allow the activation of a potentially harmful immune response in this tissue. Epicutaneous administration of a higher dose of warfarin leads to an increase in the number of neutrophilic leukocytes and intracellular myeloperoxidase activity, as well as an increase in granular CD11b + cells. In contrast to this increase, a decrease in TNF and IL-6 production as well as mRNA levels for these cytokines was observed.After administration of a higher dose of warfarin, there is a decrease in the number of mononuclear cells with an increase in the presence of CD11b + in this population, but without an effect on cytokine production, indicating the differential effects of transdermal administration of warfarin. Taken together, the results indicate the need to monitor the (adverse) effects of warfarin therapy.",
publisher = "Belgrade: Immunological Society of Serbia",
journal = "VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata",
title = "Immunomodulating effect of oral and transdermal varfarine therapy, Imunomodulatorni efekti oralne i transdermalne terapije varfarinom",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4853"
}

Mirkov, I., Popov Aleksandrov, A., Demenesku, J., Ninkov, M., Mileusnić, D., Zolotarevski, L., Subota, V., Kataranovski, D.,& Kataranovski, M.. (2016). Immunomodulating effect of oral and transdermal varfarine therapy. in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata
Belgrade: Immunological Society of Serbia., 33.
https://hdl.handle.net/21.15107/rcub_ibiss_4853

Mirkov I, Popov Aleksandrov A, Demenesku J, Ninkov M, Mileusnić D, Zolotarevski L, Subota V, Kataranovski D, Kataranovski M. Immunomodulating effect of oral and transdermal varfarine therapy. in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata. 2016;:33.
https://hdl.handle.net/21.15107/rcub_ibiss_4853 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Ninkov, Marina, Mileusnić, Dina, Zolotarevski, Lidija, Subota, Vesna, Kataranovski, Dragan, Kataranovski, Milena, "Immunomodulating effect of oral and transdermal varfarine therapy" in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata (2016):33,
https://hdl.handle.net/21.15107/rcub_ibiss_4853 .

TY  - JOUR
AU  - Zolotarevski, Lidija
AU  - Jović, Milena
AU  - Popov Aleksandrov, Aleksandra
AU  - Milosavljević, Petar
AU  - Brajušković, Goran
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://www.tandfonline.com/doi/full/10.3109/15569527.2015.1008701
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2971
AB  - CONTEXT: Skin is the target of both acute and chronic exposure to warfarin, coumarin anticoagulant. Single exposure of rat skin to this agent induces early (24 h following epicutaneous administration) local response which might be part of inflammatory/reparatory homeostatic program or introduction to pathological events in exposed skin. OBJECTIVE: To examine time-dependent changes in skin of rats exposed to epicutaneously applied warfarin. MATERIALS AND METHODS: The effect of low (10 μg) and high (100 μg) doses of warfarin on histologically evident changes of epidermis (epidermal thickness) and dermis (numbers of mesenchymal cells and dermal capillaries), skin cell proliferative activity (Ki67(+) and PCNA(+) cells) and apoptotic (TUNEL(+)) and necrotic (ultra structural appearance) cells was examined one, three and seven days after the application. RESULTS: Both warfarin doses affected the majority of skin cell activity, but with differential time-course of skin epidermal and dermal cells state/activity. The occurrence of necrotic/apoptotic epidermal and dermal cells was noted the first day after the application and the activities which point to tissue reparation/remodeling were observed seven days after skin exposure to this agent. DISCUSSION: The observed pattern of changes (early evidence of cell/tissue injury which was later followed by signs of cell activity characteristic for tissue reparation/remodeling) implied warfarin-induced toxicity in skin cells as stimulus for subsequent activities relevant for tissue homeostasis. CONCLUSION: The data presented provide new and additional information concerning skin responses to warfarin that gains access to this tissue.
T2  - Cutaneous and Ocular Toxicology
T2  - Cutaneous and Ocular Toxicology
T1  - Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.
IS  - 1
VL  - 35
DO  - 10.3109/15569527.2015.1008701
SP  - 41
EP  - 48
ER  - 

@article{
author = "Zolotarevski, Lidija and Jović, Milena and Popov Aleksandrov, Aleksandra and Milosavljević, Petar and Brajušković, Goran and Demenesku, Jelena and Mirkov, Ivana and Ninkov, Marina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "CONTEXT: Skin is the target of both acute and chronic exposure to warfarin, coumarin anticoagulant. Single exposure of rat skin to this agent induces early (24 h following epicutaneous administration) local response which might be part of inflammatory/reparatory homeostatic program or introduction to pathological events in exposed skin. OBJECTIVE: To examine time-dependent changes in skin of rats exposed to epicutaneously applied warfarin. MATERIALS AND METHODS: The effect of low (10 μg) and high (100 μg) doses of warfarin on histologically evident changes of epidermis (epidermal thickness) and dermis (numbers of mesenchymal cells and dermal capillaries), skin cell proliferative activity (Ki67(+) and PCNA(+) cells) and apoptotic (TUNEL(+)) and necrotic (ultra structural appearance) cells was examined one, three and seven days after the application. RESULTS: Both warfarin doses affected the majority of skin cell activity, but with differential time-course of skin epidermal and dermal cells state/activity. The occurrence of necrotic/apoptotic epidermal and dermal cells was noted the first day after the application and the activities which point to tissue reparation/remodeling were observed seven days after skin exposure to this agent. DISCUSSION: The observed pattern of changes (early evidence of cell/tissue injury which was later followed by signs of cell activity characteristic for tissue reparation/remodeling) implied warfarin-induced toxicity in skin cells as stimulus for subsequent activities relevant for tissue homeostasis. CONCLUSION: The data presented provide new and additional information concerning skin responses to warfarin that gains access to this tissue.",
journal = "Cutaneous and Ocular Toxicology, Cutaneous and Ocular Toxicology",
title = "Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.",
number = "1",
volume = "35",
doi = "10.3109/15569527.2015.1008701",
pages = "41-48"
}

Zolotarevski, L., Jović, M., Popov Aleksandrov, A., Milosavljević, P., Brajušković, G., Demenesku, J., Mirkov, I., Ninkov, M., Kataranovski, D.,& Kataranovski, M.. (2016). Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.. in Cutaneous and Ocular Toxicology, 35(1), 41-48.
https://doi.org/10.3109/15569527.2015.1008701

Zolotarevski L, Jović M, Popov Aleksandrov A, Milosavljević P, Brajušković G, Demenesku J, Mirkov I, Ninkov M, Kataranovski D, Kataranovski M. Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.. in Cutaneous and Ocular Toxicology. 2016;35(1):41-48.
doi:10.3109/15569527.2015.1008701 .

Zolotarevski, Lidija, Jović, Milena, Popov Aleksandrov, Aleksandra, Milosavljević, Petar, Brajušković, Goran, Demenesku, Jelena, Mirkov, Ivana, Ninkov, Marina, Kataranovski, Dragan, Kataranovski, Milena, "Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity." in Cutaneous and Ocular Toxicology, 35, no. 1 (2016):41-48,
https://doi.org/10.3109/15569527.2015.1008701 . .

TY  - JOUR
AU  - Demenesku, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S037842741630128X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2532
AB  - The impact of genetic background on effects of acute i.p. cadmium administration (0.5 mg/kg and 1 mg/kg) on basic immune activity of spleen and lungs was examined in two rat strains, Albino Oxford (AO) and Dark Agouti (DA), known to react differently to chemicals. More pronounced inhibition of Concanavalin A (ConA)-induced and Interleukin (IL)-2 stimulated spleen cell proliferation as well as higher levels of nitric oxide (known to decrease cell's proliferative ability) in DA rats at 1 mg/kg, along with greater inhibition of ConA-induced Interferon (IFN-γ)-production by total and mononuclear (MNC) spleen cells and IL-17 production by spleen MNC in DA vs. AO rats at this dose show greater susceptibility of this strain to Cd effects on spleen cells response. More pronounced infiltration of neutrophils/CD11b+ cells to lungs of DA rats treated with 1 mg/kg of Cd and decreased IL-17 lung cell responses noted solely in DA rats speaks in favor of their higher susceptibility to this metal. However, lack of strain disparity in lung cells IFN-γ responses show that there are regional differences as well. Novel data from this study depict complexity of the influence of genetic background on the effects of cadmium on host immune reactivity.
T2  - Toxicology Letters
T1  - Strain differences of cadmium-induced toxicity in rats: Insight from spleen and lung immune responses
VL  - 256
DO  - 10.1016/j.toxlet.2016.05.022
SP  - 33
EP  - 43
ER  - 

@article{
author = "Demenesku, Jelena and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Ninkov, Marina and Zolotarevski, Lidija and Kataranovski, Dragan and Brceski, Ilija and Kataranovski, Milena",
year = "2016",
abstract = "The impact of genetic background on effects of acute i.p. cadmium administration (0.5 mg/kg and 1 mg/kg) on basic immune activity of spleen and lungs was examined in two rat strains, Albino Oxford (AO) and Dark Agouti (DA), known to react differently to chemicals. More pronounced inhibition of Concanavalin A (ConA)-induced and Interleukin (IL)-2 stimulated spleen cell proliferation as well as higher levels of nitric oxide (known to decrease cell's proliferative ability) in DA rats at 1 mg/kg, along with greater inhibition of ConA-induced Interferon (IFN-γ)-production by total and mononuclear (MNC) spleen cells and IL-17 production by spleen MNC in DA vs. AO rats at this dose show greater susceptibility of this strain to Cd effects on spleen cells response. More pronounced infiltration of neutrophils/CD11b+ cells to lungs of DA rats treated with 1 mg/kg of Cd and decreased IL-17 lung cell responses noted solely in DA rats speaks in favor of their higher susceptibility to this metal. However, lack of strain disparity in lung cells IFN-γ responses show that there are regional differences as well. Novel data from this study depict complexity of the influence of genetic background on the effects of cadmium on host immune reactivity.",
journal = "Toxicology Letters",
title = "Strain differences of cadmium-induced toxicity in rats: Insight from spleen and lung immune responses",
volume = "256",
doi = "10.1016/j.toxlet.2016.05.022",
pages = "33-43"
}

Demenesku, J., Popov Aleksandrov, A., Mirkov, I., Ninkov, M., Zolotarevski, L., Kataranovski, D., Brceski, I.,& Kataranovski, M.. (2016). Strain differences of cadmium-induced toxicity in rats: Insight from spleen and lung immune responses. in Toxicology Letters, 256, 33-43.
https://doi.org/10.1016/j.toxlet.2016.05.022

Demenesku J, Popov Aleksandrov A, Mirkov I, Ninkov M, Zolotarevski L, Kataranovski D, Brceski I, Kataranovski M. Strain differences of cadmium-induced toxicity in rats: Insight from spleen and lung immune responses. in Toxicology Letters. 2016;256:33-43.
doi:10.1016/j.toxlet.2016.05.022 .

Demenesku, Jelena, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Ninkov, Marina, Zolotarevski, Lidija, Kataranovski, Dragan, Brceski, Ilija, Kataranovski, Milena, "Strain differences of cadmium-induced toxicity in rats: Insight from spleen and lung immune responses" in Toxicology Letters, 256 (2016):33-43,
https://doi.org/10.1016/j.toxlet.2016.05.022 . .

TY  - CONF
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Mileusnić, Dina
AU  - Grigorov, Ilijana
AU  - Petrović, Anja
AU  - Zolotarevski, Lidija
AU  - Nikolic, Milica
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://www.medf.kg.ac.rs/efis/Arandjelovac%20Abstract%20book%202015.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4811
AB  - Gastrointestinal (GI) tract is one of the main targets of cadmium (Cd),
important food and drinking water contaminant. In this study, the effect of
subchronic (30 days) oral (in drinking water) intake of environmentally
relevant doses of cadmium (5µg/ml and 50µg/ml) on intestinal [(tissue of
duodenum and mesenteric lymph node (mLN) cells)] was examined in
Dark Agouti (DA) rats. Atomic absorption spectrophotometry (AAS)
analysis revealed significant cadmium load in duodenum,which was
associated with changes of both intestinal bacterial load and composition
(Denaturing Gradient Gel Electrophoresis/DGGE).Shortening of villi,
damage to epithelium, increases in goblet-like vacuoles and mononuclear
cell infiltration in lamina propria were histologically evident at both
cadmium doses, with massive necrosis at higher Cd dose (judging by High
Mobility Group Box-1/HMGB-1 Western blot analysis).Increased levels of
proinflammatory cytokines (IL-1β, IFN-γ, IL-17) were observed at both Cd
doses (TNFα at higher dose solely). Cadmium administration affected
draining lymph nodes as well, judging by signs of stress response (drop of
cellular reduced glutathione/GSH at higher dose, rise of
metallothionein/MT mRNA at both doses).Increased cellularity of mLN
was observed at higher Cd dose, but with no changes in proliferative
responses. Intake of both Cd doses resulted in higher (compared to controls)
levels of IFN-γ and IL-17 mRNA as well as increased mLN cell
responsiveness to ConA stimulation.Significant increases in numbers of
CD68+ cells and stimulation of innate immune activities (numbers of
dihydrorhodamine/DHR+ cells and intracellular myeloperoxidase/MPO+
cells, LPS-stimulated nitric oxide/NO production and ex vivo IL-1β
expression) were observed at higher dose of cadmium.Proinflammatory
effects of cadmium might have resulted from changes in gut microbiota and
tissue damage.Interactions of this metal with intestinal immune system
should be taken into account when assessing dietary cadmium as health risk
factor.
PB  - Belgrade: Immunological Society of Serbia
C3  - 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging, May 24-27, 2015, Arandjelovac, Serbia
T1  - Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4811
ER  - 

@conference{
editor = "Lukić, Miodrag, Jonjic, Stipan, Nikolich-Zugich, Janko",
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Mirkov, Ivana and Mileusnić, Dina and Grigorov, Ilijana and Petrović, Anja and Zolotarevski, Lidija and Nikolic, Milica and Kataranovski, Milena",
year = "2015",
abstract = "Gastrointestinal (GI) tract is one of the main targets of cadmium (Cd),
important food and drinking water contaminant. In this study, the effect of
subchronic (30 days) oral (in drinking water) intake of environmentally
relevant doses of cadmium (5µg/ml and 50µg/ml) on intestinal [(tissue of
duodenum and mesenteric lymph node (mLN) cells)] was examined in
Dark Agouti (DA) rats. Atomic absorption spectrophotometry (AAS)
analysis revealed significant cadmium load in duodenum,which was
associated with changes of both intestinal bacterial load and composition
(Denaturing Gradient Gel Electrophoresis/DGGE).Shortening of villi,
damage to epithelium, increases in goblet-like vacuoles and mononuclear
cell infiltration in lamina propria were histologically evident at both
cadmium doses, with massive necrosis at higher Cd dose (judging by High
Mobility Group Box-1/HMGB-1 Western blot analysis).Increased levels of
proinflammatory cytokines (IL-1β, IFN-γ, IL-17) were observed at both Cd
doses (TNFα at higher dose solely). Cadmium administration affected
draining lymph nodes as well, judging by signs of stress response (drop of
cellular reduced glutathione/GSH at higher dose, rise of
metallothionein/MT mRNA at both doses).Increased cellularity of mLN
was observed at higher Cd dose, but with no changes in proliferative
responses. Intake of both Cd doses resulted in higher (compared to controls)
levels of IFN-γ and IL-17 mRNA as well as increased mLN cell
responsiveness to ConA stimulation.Significant increases in numbers of
CD68+ cells and stimulation of innate immune activities (numbers of
dihydrorhodamine/DHR+ cells and intracellular myeloperoxidase/MPO+
cells, LPS-stimulated nitric oxide/NO production and ex vivo IL-1β
expression) were observed at higher dose of cadmium.Proinflammatory
effects of cadmium might have resulted from changes in gut microbiota and
tissue damage.Interactions of this metal with intestinal immune system
should be taken into account when assessing dietary cadmium as health risk
factor.",
publisher = "Belgrade: Immunological Society of Serbia",
journal = "3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging, May 24-27, 2015, Arandjelovac, Serbia",
title = "Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4811"
}

Lukić, M., Jonjic, S., Nikolich-Zugich, J., Ninkov, M., Popov Aleksandrov, A., Demenesku, J., Mirkov, I., Mileusnić, D., Grigorov, I., Petrović, A., Zolotarevski, L., Nikolic, M.,& Kataranovski, M.. (2015). Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats. in 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging, May 24-27, 2015, Arandjelovac, Serbia
Belgrade: Immunological Society of Serbia., 48.
https://hdl.handle.net/21.15107/rcub_ibiss_4811

Lukić M, Jonjic S, Nikolich-Zugich J, Ninkov M, Popov Aleksandrov A, Demenesku J, Mirkov I, Mileusnić D, Grigorov I, Petrović A, Zolotarevski L, Nikolic M, Kataranovski M. Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats. in 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging, May 24-27, 2015, Arandjelovac, Serbia. 2015;:48.
https://hdl.handle.net/21.15107/rcub_ibiss_4811 .

Lukić, Miodrag, Jonjic, Stipan, Nikolich-Zugich, Janko, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Mirkov, Ivana, Mileusnić, Dina, Grigorov, Ilijana, Petrović, Anja, Zolotarevski, Lidija, Nikolic, Milica, Kataranovski, Milena, "Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats" in 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging, May 24-27, 2015, Arandjelovac, Serbia (2015):48,
https://hdl.handle.net/21.15107/rcub_ibiss_4811 .

TY  - JOUR
AU  - Djokic, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Mirkov, Ivana
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1984
AB  - Although numerous investigations have demonstrated a direct effect of
   cadmium (Cd) on peripheral blood mononuclear cell (PBMC) activity in
   humans, there is virtually no data concerning the in vivo impact of this
   metal on circulatory mononuclear cells. In this study, the effects of a
   sub-lethal Cd (1 mg/kg) dose were examined in rats 48 h following a
   single intraperitoneal injection. Cd treatment resulted in increased
   total peripheral blood leukocyte levels; however, decreases in PBMC
   numbers were seen. These changes coincided with an accumulation of
   mononuclear cells in the lungs and an increase in mononuclear cells
   expressing CD11b. A lack of effect of Cd on spontaneous nitric oxide
   (NO) production and on iNOS mRNA levels in the PBMC was also noted.
   Differential effects of Cd on PBMC inflammatory cytokine (IL-1 beta, TNF
   alpha, IL-6, IFN gamma, and IL-17) gene expression and production were
   also seen. Specifically, except for IL-1 beta (levels increased), there
   were decreases (relative to controls) in mRNA levels for all the other
   cytokines examined. While there were no Cd treatment-related changes in
   spontaneous production of the cytokines assessed, there seemed to be a
   trend (p = 0.06) toward a decrease in spontaneous IL-6 release. When
   these harvested cells were stimulated ex vivo, there was no effect from
   Cd exposure on LPS-stimulated IL-1 beta and TNF alpha or on
   ConA-stimulated IFN gamma or IL-17 production, but a decrease in IL-6
   production in response to LPS was, again, noted. A preliminary study
   with a lower Cd dose (0.5 mg/kg) revealed some of the same outcomes
   noted here (mononuclear cell infiltration into lungs, increases in PBMC
   IL-1 beta mRNA levels), but differential (increased IL-17 mRNA levels)
   or newly detected outcomes (increased levels of IL-1 alpha mRNA) as
   well. The described effects of the single in vivo exposure to Cd on PBMC
   might contribute to a better overall understanding of the
   immunomodulatory potential of this environmental contaminant.
T2  - Journal Of Immunotoxicology
T1  - Cadmium administration affects circulatory mononuclear cells in rats
IS  - 2
VL  - 12
DO  - 10.3109/1547691X.2014.904955
SP  - 115
EP  - 123
ER  - 

@article{
author = "Djokic, Jelena and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Mirkov, Ivana and Zolotarevski, Lidija and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2015",
abstract = "Although numerous investigations have demonstrated a direct effect of
   cadmium (Cd) on peripheral blood mononuclear cell (PBMC) activity in
   humans, there is virtually no data concerning the in vivo impact of this
   metal on circulatory mononuclear cells. In this study, the effects of a
   sub-lethal Cd (1 mg/kg) dose were examined in rats 48 h following a
   single intraperitoneal injection. Cd treatment resulted in increased
   total peripheral blood leukocyte levels; however, decreases in PBMC
   numbers were seen. These changes coincided with an accumulation of
   mononuclear cells in the lungs and an increase in mononuclear cells
   expressing CD11b. A lack of effect of Cd on spontaneous nitric oxide
   (NO) production and on iNOS mRNA levels in the PBMC was also noted.
   Differential effects of Cd on PBMC inflammatory cytokine (IL-1 beta, TNF
   alpha, IL-6, IFN gamma, and IL-17) gene expression and production were
   also seen. Specifically, except for IL-1 beta (levels increased), there
   were decreases (relative to controls) in mRNA levels for all the other
   cytokines examined. While there were no Cd treatment-related changes in
   spontaneous production of the cytokines assessed, there seemed to be a
   trend (p = 0.06) toward a decrease in spontaneous IL-6 release. When
   these harvested cells were stimulated ex vivo, there was no effect from
   Cd exposure on LPS-stimulated IL-1 beta and TNF alpha or on
   ConA-stimulated IFN gamma or IL-17 production, but a decrease in IL-6
   production in response to LPS was, again, noted. A preliminary study
   with a lower Cd dose (0.5 mg/kg) revealed some of the same outcomes
   noted here (mononuclear cell infiltration into lungs, increases in PBMC
   IL-1 beta mRNA levels), but differential (increased IL-17 mRNA levels)
   or newly detected outcomes (increased levels of IL-1 alpha mRNA) as
   well. The described effects of the single in vivo exposure to Cd on PBMC
   might contribute to a better overall understanding of the
   immunomodulatory potential of this environmental contaminant.",
journal = "Journal Of Immunotoxicology",
title = "Cadmium administration affects circulatory mononuclear cells in rats",
number = "2",
volume = "12",
doi = "10.3109/1547691X.2014.904955",
pages = "115-123"
}

Djokic, J., Popov Aleksandrov, A., Ninkov, M., Mirkov, I., Zolotarevski, L., Kataranovski, D. S.,& Kataranovski, M.. (2015). Cadmium administration affects circulatory mononuclear cells in rats. in Journal Of Immunotoxicology, 12(2), 115-123.
https://doi.org/10.3109/1547691X.2014.904955

Djokic J, Popov Aleksandrov A, Ninkov M, Mirkov I, Zolotarevski L, Kataranovski DS, Kataranovski M. Cadmium administration affects circulatory mononuclear cells in rats. in Journal Of Immunotoxicology. 2015;12(2):115-123.
doi:10.3109/1547691X.2014.904955 .

Djokic, Jelena, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Mirkov, Ivana, Zolotarevski, Lidija, Kataranovski, Dragan S., Kataranovski, Milena, "Cadmium administration affects circulatory mononuclear cells in rats" in Journal Of Immunotoxicology, 12, no. 2 (2015):115-123,
https://doi.org/10.3109/1547691X.2014.904955 . .

TY  - JOUR
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Petrovic, Anja
AU  - Grigorov, Ilijana
AU  - Zolotarevski, Lidija
AU  - Tolinacki, Maja
AU  - Kataranovski, Dragan S.
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2364
AB  - Gastrointestinal tract is one of the main targets of cadmium (Cd), an
   important food and drinking water contaminant. In the present study, the
   effect of subchronic (30 days) oral (in water) intake of 5ppm and 50ppm
   of cadmium on immune responses in the gut was examined in rats. Cadmium
   consumption resulted in reduction of bacteria corresponding to
   Lactobacillus strain, tissue damage and intestinal inflammation
   {[}increases in high mobility group box 1 (HMGB1 molecules), superoxide
   dismutase (SOD) and catalase (CAT) activity and proinflammatory cytokine
   (TNF, IL-1 beta, IFN-gamma, IL-17) content]. Draining (mesenteric) lymph
   node (MLN) stress response was observed {[}elevation of MLN glutathione
   (GSH) and metallothionein (MT) mRNA levels] and stimulation of both
   adaptive {[}cellularity, proliferation, proinflammatory (IFN-gamma and
   IL-17) MLN cell cytokine responses] as well as innate immune activity
   (increases in numbers of NK and CD68(+) cells, oxidative activities,
   IL-1 beta). In contrast to proinflammatory milieu in MLN, decreased or
   unchanged antiinflammatory IL-10 response was observed. Stimulation of
   immune activities of MLN cells have, most probably, resulted from
   sensing of cadmium-induced tissue injury, but also from bacterial
   antigens that breached compromised intestinal barrier. These effects of
   cadmium should be taken into account when assessing dietary cadmium as
   health risk factor. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Toxicology Letters
T1  - Toxicity of oral cadmium intake: Impact on gut immunity
IS  - 2
VL  - 237
DO  - 10.1016/j.toxlet.2015.06.002
SP  - 89
EP  - 99
ER  - 

@article{
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Mirkov, Ivana and Tucović, Dina and Petrovic, Anja and Grigorov, Ilijana and Zolotarevski, Lidija and Tolinacki, Maja and Kataranovski, Dragan S. and Brceski, Ilija and Kataranovski, Milena",
year = "2015",
abstract = "Gastrointestinal tract is one of the main targets of cadmium (Cd), an
   important food and drinking water contaminant. In the present study, the
   effect of subchronic (30 days) oral (in water) intake of 5ppm and 50ppm
   of cadmium on immune responses in the gut was examined in rats. Cadmium
   consumption resulted in reduction of bacteria corresponding to
   Lactobacillus strain, tissue damage and intestinal inflammation
   {[}increases in high mobility group box 1 (HMGB1 molecules), superoxide
   dismutase (SOD) and catalase (CAT) activity and proinflammatory cytokine
   (TNF, IL-1 beta, IFN-gamma, IL-17) content]. Draining (mesenteric) lymph
   node (MLN) stress response was observed {[}elevation of MLN glutathione
   (GSH) and metallothionein (MT) mRNA levels] and stimulation of both
   adaptive {[}cellularity, proliferation, proinflammatory (IFN-gamma and
   IL-17) MLN cell cytokine responses] as well as innate immune activity
   (increases in numbers of NK and CD68(+) cells, oxidative activities,
   IL-1 beta). In contrast to proinflammatory milieu in MLN, decreased or
   unchanged antiinflammatory IL-10 response was observed. Stimulation of
   immune activities of MLN cells have, most probably, resulted from
   sensing of cadmium-induced tissue injury, but also from bacterial
   antigens that breached compromised intestinal barrier. These effects of
   cadmium should be taken into account when assessing dietary cadmium as
   health risk factor. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Toxicology Letters",
title = "Toxicity of oral cadmium intake: Impact on gut immunity",
number = "2",
volume = "237",
doi = "10.1016/j.toxlet.2015.06.002",
pages = "89-99"
}

Ninkov, M., Popov Aleksandrov, A., Demenesku, J., Mirkov, I., Tucović, D., Petrovic, A., Grigorov, I., Zolotarevski, L., Tolinacki, M., Kataranovski, D. S., Brceski, I.,& Kataranovski, M.. (2015). Toxicity of oral cadmium intake: Impact on gut immunity. in Toxicology Letters, 237(2), 89-99.
https://doi.org/10.1016/j.toxlet.2015.06.002

Ninkov M, Popov Aleksandrov A, Demenesku J, Mirkov I, Tucović D, Petrovic A, Grigorov I, Zolotarevski L, Tolinacki M, Kataranovski DS, Brceski I, Kataranovski M. Toxicity of oral cadmium intake: Impact on gut immunity. in Toxicology Letters. 2015;237(2):89-99.
doi:10.1016/j.toxlet.2015.06.002 .

Ninkov, Marina, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Mirkov, Ivana, Tucović, Dina, Petrovic, Anja, Grigorov, Ilijana, Zolotarevski, Lidija, Tolinacki, Maja, Kataranovski, Dragan S., Brceski, Ilija, Kataranovski, Milena, "Toxicity of oral cadmium intake: Impact on gut immunity" in Toxicology Letters, 237, no. 2 (2015):89-99,
https://doi.org/10.1016/j.toxlet.2015.06.002 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2092
AB  - Genetic factors are among the most important determinants of
   susceptibility to induction of allergic contact dermatitis. A limited
   number of studies of experimental contact hypersensitivity (CHS) in
   animals has shown differences in the severity of CHS; however, the
   underlying mechanisms are unknown. In this study comparative analysis of
   CHS to low and high dinitrochlorobenzene (DNCB) doses regimen of
   sensitization/challenge in inbred Dark Agouti (DA) and Albino Oxford
   (AO) rats was examined. Basic aspects of draining lymph node (dLN)
   activity (cellularity, proliferation), proinflammatory (IFN-gamma,
   IL-17) and anti-inflammatory (IL-10) cytokine gene expression and
   production, as well as IL-12 and IL-23 subunits mRNA expression, were
   examined in challenge and sensitization phase of CHS reaction. Lower
   (compared to DA) intensity of CHS in AO rats was associated with lack of
   (or negligible) dLN responses in challenge phase (ex vivo, hapten- or
   IL-2-stimulated cell proliferation and proinflammatory cytokine mRNA and
   production levels) but with lack of changes in IL-10 response. Less
   pronounced dLN activity of sensitized animals of this strain was
   observed as well. Higher proliferative activity and more pronounced
   proinflammatory cytokine response during challenge and sensitization
   phase suggest these activities as underlying mechanisms of higher
   susceptibility of DA rats to CHS response to DNCB. (C) 2014 Elsevier
   Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Strain differences in contact hypersensitivity reaction to
 dinitrochlorobenzene (DNCB) in rats
VL  - 75
DO  - 10.1016/j.fct.2014.11.010
SP  - 94
EP  - 103
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Demenesku, Jelena and Ninkov, Marina and Zolotarevski, Lidija and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2015",
abstract = "Genetic factors are among the most important determinants of
   susceptibility to induction of allergic contact dermatitis. A limited
   number of studies of experimental contact hypersensitivity (CHS) in
   animals has shown differences in the severity of CHS; however, the
   underlying mechanisms are unknown. In this study comparative analysis of
   CHS to low and high dinitrochlorobenzene (DNCB) doses regimen of
   sensitization/challenge in inbred Dark Agouti (DA) and Albino Oxford
   (AO) rats was examined. Basic aspects of draining lymph node (dLN)
   activity (cellularity, proliferation), proinflammatory (IFN-gamma,
   IL-17) and anti-inflammatory (IL-10) cytokine gene expression and
   production, as well as IL-12 and IL-23 subunits mRNA expression, were
   examined in challenge and sensitization phase of CHS reaction. Lower
   (compared to DA) intensity of CHS in AO rats was associated with lack of
   (or negligible) dLN responses in challenge phase (ex vivo, hapten- or
   IL-2-stimulated cell proliferation and proinflammatory cytokine mRNA and
   production levels) but with lack of changes in IL-10 response. Less
   pronounced dLN activity of sensitized animals of this strain was
   observed as well. Higher proliferative activity and more pronounced
   proinflammatory cytokine response during challenge and sensitization
   phase suggest these activities as underlying mechanisms of higher
   susceptibility of DA rats to CHS response to DNCB. (C) 2014 Elsevier
   Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Strain differences in contact hypersensitivity reaction to
 dinitrochlorobenzene (DNCB) in rats",
volume = "75",
doi = "10.1016/j.fct.2014.11.010",
pages = "94-103"
}

Popov Aleksandrov, A., Mirkov, I., Demenesku, J., Ninkov, M., Zolotarevski, L., Kataranovski, D. S.,& Kataranovski, M.. (2015). Strain differences in contact hypersensitivity reaction to
 dinitrochlorobenzene (DNCB) in rats. in Food and Chemical Toxicology, 75, 94-103.
https://doi.org/10.1016/j.fct.2014.11.010

Popov Aleksandrov A, Mirkov I, Demenesku J, Ninkov M, Zolotarevski L, Kataranovski DS, Kataranovski M. Strain differences in contact hypersensitivity reaction to
 dinitrochlorobenzene (DNCB) in rats. in Food and Chemical Toxicology. 2015;75:94-103.
doi:10.1016/j.fct.2014.11.010 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Demenesku, Jelena, Ninkov, Marina, Zolotarevski, Lidija, Kataranovski, Dragan S., Kataranovski, Milena, "Strain differences in contact hypersensitivity reaction to
 dinitrochlorobenzene (DNCB) in rats" in Food and Chemical Toxicology, 75 (2015):94-103,
https://doi.org/10.1016/j.fct.2014.11.010 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Tusup, Marina
AU  - Mirkov, Ivana
AU  - Djokic, Jelena
AU  - Ninkov, Marina
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2045
AB  - Context: Dermal toxicity of coumarin anticoagulant rodenticides, such as
   warfarin, represents potential risk for workers handling these agents
   and for individuals applying easily available rodenticides in their
   households as well.
   Objective: In this study, proinflammatory effects of repeated
   epicutaneous administration of warfarin in rats were explored by
   examining inflammatory cytokine skin responses.
   Materials and methods: Ex vivo production of IL-1 beta, IL-6, TNF-alpha
   and IL-17 by skin explants and by epidermal cells isolated by enzyme
   (dispase/trypsin) digestion from skin repeatedly (once a day, three
   consecutive days) exposed to 10 mu g of warfarin was measured 24 h and
   72 h following the last warfarin application by ELISAs for respective
   rat cytokines.
   Results: Warfarin treatment resulted in histological changes, but skin
   or epidermal cell viability were not compromised, judging by MTT
   reduction assay. Both skin and epidermal cells responded to
   administration of this agent by production of all examined inflammatory
   cytokines (skin explants by TNF-alpha and IL-17; epidermal cells by IL-1
   beta and TNF-alpha) except IL-6. Discussion: Along with
   histomorphological changes, cytokines indicate functional consequences
   in treated skin. IL-1 beta production, that precede production of
   TNF-alpha, might be responsible for production of the latter cytokine.
   Sustained production of IL-1 beta suggests persistence of epidermal cell
   stimulation or existence of some amplification mechanisms. Requirements
   for T cells seem to exist concerning epidermal cell IL-17 production.
   Conclusion: Presented data provide additional new information concerning
   proinflammatory effects of warfarin.
T2  - Cutaneous and Ocular Toxicology
T1  - Proinflammatory cytokine responses in skin and epidermal cells following
 epicutaneous administration of anticoagulant rodenticide warfarin in
 rats
IS  - 2
VL  - 34
DO  - 10.3109/15569527.2014.928307
SP  - 149
EP  - 155
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Tusup, Marina and Mirkov, Ivana and Djokic, Jelena and Ninkov, Marina and Zolotarevski, Lidija and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2015",
abstract = "Context: Dermal toxicity of coumarin anticoagulant rodenticides, such as
   warfarin, represents potential risk for workers handling these agents
   and for individuals applying easily available rodenticides in their
   households as well.
   Objective: In this study, proinflammatory effects of repeated
   epicutaneous administration of warfarin in rats were explored by
   examining inflammatory cytokine skin responses.
   Materials and methods: Ex vivo production of IL-1 beta, IL-6, TNF-alpha
   and IL-17 by skin explants and by epidermal cells isolated by enzyme
   (dispase/trypsin) digestion from skin repeatedly (once a day, three
   consecutive days) exposed to 10 mu g of warfarin was measured 24 h and
   72 h following the last warfarin application by ELISAs for respective
   rat cytokines.
   Results: Warfarin treatment resulted in histological changes, but skin
   or epidermal cell viability were not compromised, judging by MTT
   reduction assay. Both skin and epidermal cells responded to
   administration of this agent by production of all examined inflammatory
   cytokines (skin explants by TNF-alpha and IL-17; epidermal cells by IL-1
   beta and TNF-alpha) except IL-6. Discussion: Along with
   histomorphological changes, cytokines indicate functional consequences
   in treated skin. IL-1 beta production, that precede production of
   TNF-alpha, might be responsible for production of the latter cytokine.
   Sustained production of IL-1 beta suggests persistence of epidermal cell
   stimulation or existence of some amplification mechanisms. Requirements
   for T cells seem to exist concerning epidermal cell IL-17 production.
   Conclusion: Presented data provide additional new information concerning
   proinflammatory effects of warfarin.",
journal = "Cutaneous and Ocular Toxicology",
title = "Proinflammatory cytokine responses in skin and epidermal cells following
 epicutaneous administration of anticoagulant rodenticide warfarin in
 rats",
number = "2",
volume = "34",
doi = "10.3109/15569527.2014.928307",
pages = "149-155"
}

Popov Aleksandrov, A., Tusup, M., Mirkov, I., Djokic, J., Ninkov, M., Zolotarevski, L., Kataranovski, D. S.,& Kataranovski, M.. (2015). Proinflammatory cytokine responses in skin and epidermal cells following
 epicutaneous administration of anticoagulant rodenticide warfarin in
 rats. in Cutaneous and Ocular Toxicology, 34(2), 149-155.
https://doi.org/10.3109/15569527.2014.928307

Popov Aleksandrov A, Tusup M, Mirkov I, Djokic J, Ninkov M, Zolotarevski L, Kataranovski DS, Kataranovski M. Proinflammatory cytokine responses in skin and epidermal cells following
 epicutaneous administration of anticoagulant rodenticide warfarin in
 rats. in Cutaneous and Ocular Toxicology. 2015;34(2):149-155.
doi:10.3109/15569527.2014.928307 .

Popov Aleksandrov, Aleksandra, Tusup, Marina, Mirkov, Ivana, Djokic, Jelena, Ninkov, Marina, Zolotarevski, Lidija, Kataranovski, Dragan S., Kataranovski, Milena, "Proinflammatory cytokine responses in skin and epidermal cells following
 epicutaneous administration of anticoagulant rodenticide warfarin in
 rats" in Cutaneous and Ocular Toxicology, 34, no. 2 (2015):149-155,
https://doi.org/10.3109/15569527.2014.928307 . .

TY  - CONF
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Mileusnić, Dina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4808
AB  - Introduction: Conflicting data (suppression, augmentation, no effect) exist concerning cadmium (Cd) effects on immune system depending on activity and tissue examined. This study
investigates responses to acute Cd intoxication in three compartments (peripheral blood, spleen and lungs) in Dark Agouti (DA) and Albino Oxford (AO) rats, which are differently
susceptible to variety of stimuli.
Materials and Methods: Systemic (IL-6, TNF, acute phase proteins) and tissue responses [cell stress (metallothionein/MT gene expression), CD11b expression, and cytokine (IFN-γ,
IL-17, IL-10) production and mRNA expression] were measured following intraperitoneal (1 mg/kg) Cd administration.
Results: Cd induces systemic inflammatory response with similar intensity in both rat strains. Increase in Cd spleen content and MT expression evident in both strains (higher in DA
compared to AO rats) was followed by increase in neutrophil infiltration and CD11b expression (with same intensity). Although in both strains Cd caused decreased IFN-γ, unchanged
IL-17 and lower IL-10 responsiveness (compared to respective control), decrease of IFN-γ was more intense in DA compared to AO rats. In lungs of both strains increased Cd deposition
and MT expression (higher in AO) as well as neutrophil infiltration and CD11b expression (greater in DA) was observed. While decreased IFN-γ was noted in both strains, lower IL-17
and IL-10 (vs. controls) were evident in DA rats solely.
Conclusions: Acute Cd intoxication exerts strain-related effects (both inflammatory and immunosuppressive) depending on tissue and activity investigated, but the effects are more
pronounced in DA rats.
PB  - European Federation of Immunological Societies
C3  - 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 353.
T1  - Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue
SP  - 353
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4808
ER  - 

@conference{
author = "Demenesku, Jelena and Mirkov, Ivana and Ninkov, Marina and Popov Aleksandrov, Aleksandra and Zolotarevski, Lidija and Subota, Vesna and Mileusnić, Dina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2015",
abstract = "Introduction: Conflicting data (suppression, augmentation, no effect) exist concerning cadmium (Cd) effects on immune system depending on activity and tissue examined. This study
investigates responses to acute Cd intoxication in three compartments (peripheral blood, spleen and lungs) in Dark Agouti (DA) and Albino Oxford (AO) rats, which are differently
susceptible to variety of stimuli.
Materials and Methods: Systemic (IL-6, TNF, acute phase proteins) and tissue responses [cell stress (metallothionein/MT gene expression), CD11b expression, and cytokine (IFN-γ,
IL-17, IL-10) production and mRNA expression] were measured following intraperitoneal (1 mg/kg) Cd administration.
Results: Cd induces systemic inflammatory response with similar intensity in both rat strains. Increase in Cd spleen content and MT expression evident in both strains (higher in DA
compared to AO rats) was followed by increase in neutrophil infiltration and CD11b expression (with same intensity). Although in both strains Cd caused decreased IFN-γ, unchanged
IL-17 and lower IL-10 responsiveness (compared to respective control), decrease of IFN-γ was more intense in DA compared to AO rats. In lungs of both strains increased Cd deposition
and MT expression (higher in AO) as well as neutrophil infiltration and CD11b expression (greater in DA) was observed. While decreased IFN-γ was noted in both strains, lower IL-17
and IL-10 (vs. controls) were evident in DA rats solely.
Conclusions: Acute Cd intoxication exerts strain-related effects (both inflammatory and immunosuppressive) depending on tissue and activity investigated, but the effects are more
pronounced in DA rats.",
publisher = "European Federation of Immunological Societies",
journal = "4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 353.",
title = "Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue",
pages = "353",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4808"
}

Demenesku, J., Mirkov, I., Ninkov, M., Popov Aleksandrov, A., Zolotarevski, L., Subota, V., Mileusnić, D., Kataranovski, D.,& Kataranovski, M.. (2015). Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 353.
European Federation of Immunological Societies., 353.
https://hdl.handle.net/21.15107/rcub_ibiss_4808

Demenesku J, Mirkov I, Ninkov M, Popov Aleksandrov A, Zolotarevski L, Subota V, Mileusnić D, Kataranovski D, Kataranovski M. Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 353.. 2015;:353.
https://hdl.handle.net/21.15107/rcub_ibiss_4808 .

Demenesku, Jelena, Mirkov, Ivana, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Zolotarevski, Lidija, Subota, Vesna, Mileusnić, Dina, Kataranovski, Dragan, Kataranovski, Milena, "Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue" in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 353. (2015):353,
https://hdl.handle.net/21.15107/rcub_ibiss_4808 .

TY  - CONF
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Mileusnić, Dina
AU  - Tolinacki, Maja
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4809
AB  - Introduction: Toxic metal cadmium (Cd) is important food and drinking water contaminant. The majority of ingested cadmium retained in the gastrointestinal tract (GIT) mucosa
which allocates GIT as its main target. Mechanisms of induction of intestinal inflammatory response are largely unknown.
Materials and Methods: Effect of subchronic (30 days) oral (in water) cadmium administration (5 ppm and 50 ppm) was examined in Dark Agouti (DA) and Albino Oxford (AO) rats.
Beside intestinal immune response, activity of draining mesenteric lymph node (MLN) cells, central place for induction of intestinal immune tolerance and local protective responses,
was evaluated.
Results: In both rat strains cadmium consumption resulted in reduction of bacteria (Lactobacillus strain), intestinal tissue damage, modulated antioxidant enzymes activity and
inflammation [increased proinflammatory cytokine (TNF, IL-1β, IFN-γ, IL-17) content in DA rats; increased TNF and IL-10 content in AO rats] in duodenal homogenates. Accumulation
of cadmium in MLN was followed by stress response [elevation of MLN glutathione and metallothionein mRNA levels] only in DA rats. Stimulation of both adaptive (proliferation, Th1
and Th17 cytokine response) and innate immune activities (NKG2D+, CD68+ cells, selected oxidative activities, IL-1β) in MLN was observed, more pronounced in DA compared to AO
rats.
Conclusions: Oral intake of cadmium resulted in intestinal damage, inflammation, and induction of proinflammatory milleu and innate effector cell activities in MLN. Cadmiuminduced proinflammatory responses in DA rats but discrete immune responses of AO rats imply strain-dependent effects of oral cadmium administration.
PB  - European Federation of Immunological Societies
C3  - 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.
T1  - Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes
SP  - 486
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4809
ER  - 

@conference{
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Mirkov, Ivana and Mileusnić, Dina and Tolinacki, Maja and Zolotarevski, Lidija and Kataranovski, Dragan and Brceski, Ilija and Kataranovski, Milena",
year = "2015",
abstract = "Introduction: Toxic metal cadmium (Cd) is important food and drinking water contaminant. The majority of ingested cadmium retained in the gastrointestinal tract (GIT) mucosa
which allocates GIT as its main target. Mechanisms of induction of intestinal inflammatory response are largely unknown.
Materials and Methods: Effect of subchronic (30 days) oral (in water) cadmium administration (5 ppm and 50 ppm) was examined in Dark Agouti (DA) and Albino Oxford (AO) rats.
Beside intestinal immune response, activity of draining mesenteric lymph node (MLN) cells, central place for induction of intestinal immune tolerance and local protective responses,
was evaluated.
Results: In both rat strains cadmium consumption resulted in reduction of bacteria (Lactobacillus strain), intestinal tissue damage, modulated antioxidant enzymes activity and
inflammation [increased proinflammatory cytokine (TNF, IL-1β, IFN-γ, IL-17) content in DA rats; increased TNF and IL-10 content in AO rats] in duodenal homogenates. Accumulation
of cadmium in MLN was followed by stress response [elevation of MLN glutathione and metallothionein mRNA levels] only in DA rats. Stimulation of both adaptive (proliferation, Th1
and Th17 cytokine response) and innate immune activities (NKG2D+, CD68+ cells, selected oxidative activities, IL-1β) in MLN was observed, more pronounced in DA compared to AO
rats.
Conclusions: Oral intake of cadmium resulted in intestinal damage, inflammation, and induction of proinflammatory milleu and innate effector cell activities in MLN. Cadmiuminduced proinflammatory responses in DA rats but discrete immune responses of AO rats imply strain-dependent effects of oral cadmium administration.",
publisher = "European Federation of Immunological Societies",
journal = "4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.",
title = "Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes",
pages = "486",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4809"
}

Ninkov, M., Popov Aleksandrov, A., Demenesku, J., Mirkov, I., Mileusnić, D., Tolinacki, M., Zolotarevski, L., Kataranovski, D., Brceski, I.,& Kataranovski, M.. (2015). Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.
European Federation of Immunological Societies., 486.
https://hdl.handle.net/21.15107/rcub_ibiss_4809

Ninkov M, Popov Aleksandrov A, Demenesku J, Mirkov I, Mileusnić D, Tolinacki M, Zolotarevski L, Kataranovski D, Brceski I, Kataranovski M. Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.. 2015;:486.
https://hdl.handle.net/21.15107/rcub_ibiss_4809 .

Ninkov, Marina, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Mirkov, Ivana, Mileusnić, Dina, Tolinacki, Maja, Zolotarevski, Lidija, Kataranovski, Dragan, Brceski, Ilija, Kataranovski, Milena, "Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes" in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486. (2015):486,
https://hdl.handle.net/21.15107/rcub_ibiss_4809 .

TY  - CONF
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Mileusnić, Dina
AU  - Zolotarevski, Lidija
AU  - Tolinački, Maja
AU  - Kataranovski, Dragan
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4810
AB  - Conflicting data exist concerning cadmium
effects on immune system depending on the
experimental model, exposure or tissue/activity
examined. In this study immunotoxicity of acute
intraperitoneal and oral cadmium
administration was investigated in rats. The
use of the inflammation-prone inbred Dark
Agouti (DA) and less reactive Albino Oxford
(AO) rats showed differential (immune activityrelated and/or strain-related) effects of
cadmium (1 mg of Cd/kg, i.p.) on spleen
immune responses. A decrease in ConAinduced proliferation (related to altered spleen
cells responsiveness to IL-2) and of IFN-γ
(independently of IL-4 and IL-10) was more
pronounced in DA rats. Increased innate
immunity splenocyte activity (granulocyte
CD11b+ cells, iNOS mRNA and NO
production, myeloperoxidase MPO activity, IL1β mRNA and IL-1β protein product levels)
were observed in both strains (some of them
more pronounced in DA rats), while a decrease
in respiratory burst (dihydrorhodamine/DHR
oxidation) was similar. 30-day oral intake of 5
ppm and 50 ppm of cadmium by DA rats
resulted in reduction of some probiotic bacteria,
villous damage and intestinal inflammation
[(increased levels of High Mobility Group
Box1/HMGB1, antioxidant enzyme (superoxide
dismutase/SOD and catalase/CAT) activity and
proinflammatory cytokine (TNF, IL-1β, IFN-γ,
IL-17) in gut homogenates]. Stimulation of both
adaptive (increased cellularity, proliferation,
IFN-γ and IL-17cytokine responses) as well as
innate immune activity (increases in numbers
of NK cells and M1-like macrophages,
oxidative cell activities, IL-1β) of gut draining
(mesenteric) lymph nodes was associated with
decreased or unchanged antiinflammatory
cytokine (IL-10) cell response. Differential
(immunosuppressive and immunostimulatory)
effects noted in the same tissue (spleen)
should be taken into account when exploring
immunotoxicity of this metal. Stimulation of gut
immune responses imply dietary cadmium as
health risk factor.
PB  - Department of Biomedical Sciences-Histology of the University of Sassari
C3  - II Cadmium Symposium, June 25-27, 2015, Sassari, Italy
T1  - Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4810
ER  - 

@conference{
author = "Demenesku, Jelena and Ninkov, Marina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Mileusnić, Dina and Zolotarevski, Lidija and Tolinački, Maja and Kataranovski, Dragan and Brceski, Ilija and Kataranovski, Milena",
year = "2015",
abstract = "Conflicting data exist concerning cadmium
effects on immune system depending on the
experimental model, exposure or tissue/activity
examined. In this study immunotoxicity of acute
intraperitoneal and oral cadmium
administration was investigated in rats. The
use of the inflammation-prone inbred Dark
Agouti (DA) and less reactive Albino Oxford
(AO) rats showed differential (immune activityrelated and/or strain-related) effects of
cadmium (1 mg of Cd/kg, i.p.) on spleen
immune responses. A decrease in ConAinduced proliferation (related to altered spleen
cells responsiveness to IL-2) and of IFN-γ
(independently of IL-4 and IL-10) was more
pronounced in DA rats. Increased innate
immunity splenocyte activity (granulocyte
CD11b+ cells, iNOS mRNA and NO
production, myeloperoxidase MPO activity, IL1β mRNA and IL-1β protein product levels)
were observed in both strains (some of them
more pronounced in DA rats), while a decrease
in respiratory burst (dihydrorhodamine/DHR
oxidation) was similar. 30-day oral intake of 5
ppm and 50 ppm of cadmium by DA rats
resulted in reduction of some probiotic bacteria,
villous damage and intestinal inflammation
[(increased levels of High Mobility Group
Box1/HMGB1, antioxidant enzyme (superoxide
dismutase/SOD and catalase/CAT) activity and
proinflammatory cytokine (TNF, IL-1β, IFN-γ,
IL-17) in gut homogenates]. Stimulation of both
adaptive (increased cellularity, proliferation,
IFN-γ and IL-17cytokine responses) as well as
innate immune activity (increases in numbers
of NK cells and M1-like macrophages,
oxidative cell activities, IL-1β) of gut draining
(mesenteric) lymph nodes was associated with
decreased or unchanged antiinflammatory
cytokine (IL-10) cell response. Differential
(immunosuppressive and immunostimulatory)
effects noted in the same tissue (spleen)
should be taken into account when exploring
immunotoxicity of this metal. Stimulation of gut
immune responses imply dietary cadmium as
health risk factor.",
publisher = "Department of Biomedical Sciences-Histology of the University of Sassari",
journal = "II Cadmium Symposium, June 25-27, 2015, Sassari, Italy",
title = "Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats",
pages = "31",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4810"
}

Demenesku, J., Ninkov, M., Popov Aleksandrov, A., Mirkov, I., Mileusnić, D., Zolotarevski, L., Tolinački, M., Kataranovski, D., Brceski, I.,& Kataranovski, M.. (2015). Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats. in II Cadmium Symposium, June 25-27, 2015, Sassari, Italy
Department of Biomedical Sciences-Histology of the University of Sassari., 31.
https://hdl.handle.net/21.15107/rcub_ibiss_4810

Demenesku J, Ninkov M, Popov Aleksandrov A, Mirkov I, Mileusnić D, Zolotarevski L, Tolinački M, Kataranovski D, Brceski I, Kataranovski M. Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats. in II Cadmium Symposium, June 25-27, 2015, Sassari, Italy. 2015;:31.
https://hdl.handle.net/21.15107/rcub_ibiss_4810 .

Demenesku, Jelena, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Mileusnić, Dina, Zolotarevski, Lidija, Tolinački, Maja, Kataranovski, Dragan, Brceski, Ilija, Kataranovski, Milena, "Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats" in II Cadmium Symposium, June 25-27, 2015, Sassari, Italy (2015):31,
https://hdl.handle.net/21.15107/rcub_ibiss_4810 .

TY  - JOUR
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2103
AB  - Conflicting data (both suppression and augmentation as well as lack of
   the effect) exist in respect to cadmium (Cd) and splenic T cell-based
   immune cell activity. Spleen is also the site of innate immune responses
   but impact of Cd on this type of immunity has been less explored. In the
   present study the effects of acute Cd administration on basic aspects of
   both T cell-based and innate immune spleen cell activity were examined
   in rats. Intraperitoneal injection of 1 mg of Cd/kg resulted in decrease
   in concanavalin A (ConA) induced proliferation which seems to be more
   related to altered spleen cells responsiveness to IL-2 than to
   apoptosis. Differential effects on proinflammatory T cell derived
   cytokines were observed (decreases of IFN-gamma gene expression and
   ConA-stimulated production, but increases in IL-17 mRNA levels with no
   effect on concentrations of protein product). Reduction of IFN-gamma
   production seemed not to rely on IL-4 and IL-10, but at least partly on
   nitric oxide (NO). Increased activity relevant for innate immunity
   (granulocyte and CD11b(+) cell accumulation in the spleen, inducible
   nitric oxide synthase/iNOS expression and NO production by spleen cells)
   was observed, but there was a decrease in respiratory burst
   (dihydrorhodamine/DHR oxidation and nitroblue tetrazolium/NBT
   reduction). Increases of TNF-alpha and IL-1 beta gene expression and
   IL-1 beta protein product were noted as well. Administration of 0.5 mg
   Cd/kg resulted in less pronounced (ConA-induced proliferation) or lack
   of the effect (IFN-gamma production) on spleen T cell activities and on
   innate activities (granulocyte accumulation, NO production) as well.
   However, increases of spleen cell respiratory burst activity and IL-1
   beta production were observed. Effects of lower cadmium doses (5 ppm and
   50 ppm) on several aspects of spleen cell immune activity were observed
   in intermediate period of exposure (30 days, oral intake) as well.
   Differential effects of Cd on immune activities of spleen cells might
   contribute to our understanding of the complexity of immunomodulatory
   effects of this metal. (C) 2014 Elsevier Ireland Ltd. All rights
   reserved.
T2  - Toxicology
T1  - Acute cadmium administration to rats exerts both immunosuppressive and
 proinflammatory effects in spleen
VL  - 326
DO  - 10.1016/j.tox.2014.10.012
SP  - 96
EP  - 108
ER  - 

@article{
author = "Demenesku, Jelena and Mirkov, Ivana and Ninkov, Marina and Popov Aleksandrov, Aleksandra and Zolotarevski, Lidija and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2014",
abstract = "Conflicting data (both suppression and augmentation as well as lack of
   the effect) exist in respect to cadmium (Cd) and splenic T cell-based
   immune cell activity. Spleen is also the site of innate immune responses
   but impact of Cd on this type of immunity has been less explored. In the
   present study the effects of acute Cd administration on basic aspects of
   both T cell-based and innate immune spleen cell activity were examined
   in rats. Intraperitoneal injection of 1 mg of Cd/kg resulted in decrease
   in concanavalin A (ConA) induced proliferation which seems to be more
   related to altered spleen cells responsiveness to IL-2 than to
   apoptosis. Differential effects on proinflammatory T cell derived
   cytokines were observed (decreases of IFN-gamma gene expression and
   ConA-stimulated production, but increases in IL-17 mRNA levels with no
   effect on concentrations of protein product). Reduction of IFN-gamma
   production seemed not to rely on IL-4 and IL-10, but at least partly on
   nitric oxide (NO). Increased activity relevant for innate immunity
   (granulocyte and CD11b(+) cell accumulation in the spleen, inducible
   nitric oxide synthase/iNOS expression and NO production by spleen cells)
   was observed, but there was a decrease in respiratory burst
   (dihydrorhodamine/DHR oxidation and nitroblue tetrazolium/NBT
   reduction). Increases of TNF-alpha and IL-1 beta gene expression and
   IL-1 beta protein product were noted as well. Administration of 0.5 mg
   Cd/kg resulted in less pronounced (ConA-induced proliferation) or lack
   of the effect (IFN-gamma production) on spleen T cell activities and on
   innate activities (granulocyte accumulation, NO production) as well.
   However, increases of spleen cell respiratory burst activity and IL-1
   beta production were observed. Effects of lower cadmium doses (5 ppm and
   50 ppm) on several aspects of spleen cell immune activity were observed
   in intermediate period of exposure (30 days, oral intake) as well.
   Differential effects of Cd on immune activities of spleen cells might
   contribute to our understanding of the complexity of immunomodulatory
   effects of this metal. (C) 2014 Elsevier Ireland Ltd. All rights
   reserved.",
journal = "Toxicology",
title = "Acute cadmium administration to rats exerts both immunosuppressive and
 proinflammatory effects in spleen",
volume = "326",
doi = "10.1016/j.tox.2014.10.012",
pages = "96-108"
}

Demenesku, J., Mirkov, I., Ninkov, M., Popov Aleksandrov, A., Zolotarevski, L., Kataranovski, D. S.,& Kataranovski, M.. (2014). Acute cadmium administration to rats exerts both immunosuppressive and
 proinflammatory effects in spleen. in Toxicology, 326, 96-108.
https://doi.org/10.1016/j.tox.2014.10.012

Demenesku J, Mirkov I, Ninkov M, Popov Aleksandrov A, Zolotarevski L, Kataranovski DS, Kataranovski M. Acute cadmium administration to rats exerts both immunosuppressive and
 proinflammatory effects in spleen. in Toxicology. 2014;326:96-108.
doi:10.1016/j.tox.2014.10.012 .

Demenesku, Jelena, Mirkov, Ivana, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Zolotarevski, Lidija, Kataranovski, Dragan S., Kataranovski, Milena, "Acute cadmium administration to rats exerts both immunosuppressive and
 proinflammatory effects in spleen" in Toxicology, 326 (2014):96-108,
https://doi.org/10.1016/j.tox.2014.10.012 . .

TY  - JOUR
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Jovanović Stojanov, Sofija
AU  - Petrović, Anja
AU  - Ivanović Matić, Svetlana
AU  - Zolotarevski, Lidija
AU  - Poznanović, Goran
AU  - Martinović, Vesna
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2210
AB  - Oxidative stress-mediated damage to liver tissue underlies the
   pathological alterations in liver morphology and function that are
   observed in diabetes. We examined the effects of the antioxidant action
   of melatonin against necrosis-inducing DNA damage in hepatocytes of
   streptozotocin (STZ)-induced diabetic rats. Daily administration of
   melatonin (0.2 mg/kg) was initiated 3 days before diabetes induction and
   maintained for 4 weeks. Melatonin-treated diabetic rats exhibited
   improved markers of liver injury (P<0.05), alkaline phosphatase, and
   alanine and aspartate aminotransferases. Melatonin prevented the
   diabetes-related morphological deterioration of hepatocytes, DNA damage
   (P<0.05), and hepatocellular necrosis. The improvement was due to
   containment of the pronecrotic oxygen radical load, observed as
   inhibition (P<0.05) of the diabetes-induced rise in lipid peroxidation
   and hydrogen peroxide increase in the liver. This was accompanied by
   improved necrotic markers of cellular damage: a significant reduction in
   cleavage of the DNA repair enzyme poly(ADP-ribose) polymerase 1 (PARP-1)
   into necrotic 55- and 62-kDa fragments, and inhibition of
   nucleus-to-cytoplasm translocation and accumulation in the serum of the
   high-mobility group box 1 (HMGB1) protein. We conclude that melatonin is
   hepatoprotective in diabetes. It reduces extensive DNA damage and
   resulting necrotic processes. Melatonin application could thus present a
   viable therapeutic option in the management of diabetes-induced liver
   injury.
T2  - Journal of Physiology and Biochemistry
T1  - Hepatoprotective effects of melatonin against pronecrotic cellular
 events in streptozotocin-induced diabetic rats
IS  - 2
VL  - 70
DO  - 10.1007/s13105-014-0322-7
SP  - 441
EP  - 450
ER  - 

@article{
author = "Grigorov, Ilijana and Bogojević, Desanka and Jovanović Stojanov, Sofija and Petrović, Anja and Ivanović Matić, Svetlana and Zolotarevski, Lidija and Poznanović, Goran and Martinović, Vesna",
year = "2014",
abstract = "Oxidative stress-mediated damage to liver tissue underlies the
   pathological alterations in liver morphology and function that are
   observed in diabetes. We examined the effects of the antioxidant action
   of melatonin against necrosis-inducing DNA damage in hepatocytes of
   streptozotocin (STZ)-induced diabetic rats. Daily administration of
   melatonin (0.2 mg/kg) was initiated 3 days before diabetes induction and
   maintained for 4 weeks. Melatonin-treated diabetic rats exhibited
   improved markers of liver injury (P<0.05), alkaline phosphatase, and
   alanine and aspartate aminotransferases. Melatonin prevented the
   diabetes-related morphological deterioration of hepatocytes, DNA damage
   (P<0.05), and hepatocellular necrosis. The improvement was due to
   containment of the pronecrotic oxygen radical load, observed as
   inhibition (P<0.05) of the diabetes-induced rise in lipid peroxidation
   and hydrogen peroxide increase in the liver. This was accompanied by
   improved necrotic markers of cellular damage: a significant reduction in
   cleavage of the DNA repair enzyme poly(ADP-ribose) polymerase 1 (PARP-1)
   into necrotic 55- and 62-kDa fragments, and inhibition of
   nucleus-to-cytoplasm translocation and accumulation in the serum of the
   high-mobility group box 1 (HMGB1) protein. We conclude that melatonin is
   hepatoprotective in diabetes. It reduces extensive DNA damage and
   resulting necrotic processes. Melatonin application could thus present a
   viable therapeutic option in the management of diabetes-induced liver
   injury.",
journal = "Journal of Physiology and Biochemistry",
title = "Hepatoprotective effects of melatonin against pronecrotic cellular
 events in streptozotocin-induced diabetic rats",
number = "2",
volume = "70",
doi = "10.1007/s13105-014-0322-7",
pages = "441-450"
}

Grigorov, I., Bogojević, D., Jovanović Stojanov, S., Petrović, A., Ivanović Matić, S., Zolotarevski, L., Poznanović, G.,& Martinović, V.. (2014). Hepatoprotective effects of melatonin against pronecrotic cellular
 events in streptozotocin-induced diabetic rats. in Journal of Physiology and Biochemistry, 70(2), 441-450.
https://doi.org/10.1007/s13105-014-0322-7

Grigorov I, Bogojević D, Jovanović Stojanov S, Petrović A, Ivanović Matić S, Zolotarevski L, Poznanović G, Martinović V. Hepatoprotective effects of melatonin against pronecrotic cellular
 events in streptozotocin-induced diabetic rats. in Journal of Physiology and Biochemistry. 2014;70(2):441-450.
doi:10.1007/s13105-014-0322-7 .

Grigorov, Ilijana, Bogojević, Desanka, Jovanović Stojanov, Sofija, Petrović, Anja, Ivanović Matić, Svetlana, Zolotarevski, Lidija, Poznanović, Goran, Martinović, Vesna, "Hepatoprotective effects of melatonin against pronecrotic cellular
 events in streptozotocin-induced diabetic rats" in Journal of Physiology and Biochemistry, 70, no. 2 (2014):441-450,
https://doi.org/10.1007/s13105-014-0322-7 . .

TY  - JOUR
AU  - Kataranovski, Milena
AU  - Zolotarevski, Lidija
AU  - Belij, Sandra
AU  - Mirkov, Ivana
AU  - Stošić, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Kataranovski, D.
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/257
AB  - The nematode Calodium hepaticum and the cestode Taenia taeniaeformis are zoonotic helminths primarly found in the liver of common wild rats. Most reports on these helminth species with cosmopolitan distribution are from Asia, and there is paucity of data for Europe. Wild Norway rats (Rattus norvegicus) from urban and suburban habitats of the Belgrade area were examined for the presence of Calodium hepaticum and Taenia taeniaeformis larvae liver infections. The presence of visible cysts and a histomorphology of parasite-related inflammatory liver responses were sought as signs of infection. The total prevalence of infection was 10.9% (C. hepaticum) and 29.9% (T. taeniaeformis), with no differences between the sexes. No difference in the annual prevalence of both helminth species was noted. Data obtained in this study provide new information relevant to wild Norway rats as sources of C. hepaticum and T. taeniaeformis liver infection in this geographic area, and, in a wider context, in Europe. .
T2  - Archives of Biological Sciences
T1  - Prvi nalazi infekcije jetre parazitima Calodium hepaticum i Taenia taeniaeformis kod sivog pacova (Rattus norvegicus) u Srbiji
T1  - First record of Calodium hepaticum and Taenia taeniaeformis liver infection in wild Norway rats (Rattus norvegicus) in Serbia
IS  - 2
VL  - 62
SP  - 431
EP  - 440
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_257
ER  - 

@article{
author = "Kataranovski, Milena and Zolotarevski, Lidija and Belij, Sandra and Mirkov, Ivana and Stošić, Jelena and Popov Aleksandrov, Aleksandra and Kataranovski, D.",
year = "2010, 2010",
abstract = "The nematode Calodium hepaticum and the cestode Taenia taeniaeformis are zoonotic helminths primarly found in the liver of common wild rats. Most reports on these helminth species with cosmopolitan distribution are from Asia, and there is paucity of data for Europe. Wild Norway rats (Rattus norvegicus) from urban and suburban habitats of the Belgrade area were examined for the presence of Calodium hepaticum and Taenia taeniaeformis larvae liver infections. The presence of visible cysts and a histomorphology of parasite-related inflammatory liver responses were sought as signs of infection. The total prevalence of infection was 10.9% (C. hepaticum) and 29.9% (T. taeniaeformis), with no differences between the sexes. No difference in the annual prevalence of both helminth species was noted. Data obtained in this study provide new information relevant to wild Norway rats as sources of C. hepaticum and T. taeniaeformis liver infection in this geographic area, and, in a wider context, in Europe. .",
journal = "Archives of Biological Sciences",
title = "Prvi nalazi infekcije jetre parazitima Calodium hepaticum i Taenia taeniaeformis kod sivog pacova (Rattus norvegicus) u Srbiji, First record of Calodium hepaticum and Taenia taeniaeformis liver infection in wild Norway rats (Rattus norvegicus) in Serbia",
number = "2",
volume = "62",
pages = "431-440",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_257"
}

Kataranovski, M., Zolotarevski, L., Belij, S., Mirkov, I., Stošić, J., Popov Aleksandrov, A.,& Kataranovski, D.. (2010). Prvi nalazi infekcije jetre parazitima Calodium hepaticum i Taenia taeniaeformis kod sivog pacova (Rattus norvegicus) u Srbiji. in Archives of Biological Sciences, 62(2), 431-440.
https://hdl.handle.net/21.15107/rcub_ibiss_257

Kataranovski M, Zolotarevski L, Belij S, Mirkov I, Stošić J, Popov Aleksandrov A, Kataranovski D. Prvi nalazi infekcije jetre parazitima Calodium hepaticum i Taenia taeniaeformis kod sivog pacova (Rattus norvegicus) u Srbiji. in Archives of Biological Sciences. 2010;62(2):431-440.
https://hdl.handle.net/21.15107/rcub_ibiss_257 .

Kataranovski, Milena, Zolotarevski, Lidija, Belij, Sandra, Mirkov, Ivana, Stošić, Jelena, Popov Aleksandrov, Aleksandra, Kataranovski, D., "Prvi nalazi infekcije jetre parazitima Calodium hepaticum i Taenia taeniaeformis kod sivog pacova (Rattus norvegicus) u Srbiji" in Archives of Biological Sciences, 62, no. 2 (2010):431-440,
https://hdl.handle.net/21.15107/rcub_ibiss_257 .

TY  - JOUR
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
AU  - Zolotarevski, Lidija
AU  - Popov Aleksandrov, Aleksandra
AU  - Belij, Sandra
AU  - Stošić, Jelena
AU  - Kataranovski, D.
PY  - 2009
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/239
AB  - Basic parameters of spleen immune activity (spleen weight, histomorphology of splenic compartments, and mitogen-induced splenocyte proliferative capacity in vitro) were evaluated in adult individuals of wild Norway rats from urban habitats and compared to the same data obtained in laboratory rat strains. A wider range of relative spleen mass and differential histomorphological characteristics, together with differences in the level and pattern of responsiveness of splenocytes to exogenous stimulation, were noted in spleens of wild Norway rats. Evidence of both enhanced and low-level immune-relevant spleen activity in wild rats demonstrates the complexity of changes in spleen immune activity in rats from natural populations.
AB  - Ispitivani su osnovni parametri imunske aktivnosti u slezini (masa slezine, histomorfologija i sposobnost ćelija slezine da profilerišu u odgovoru na mitogen u in vitro uslovima) kod adultnih jedinki sivog pacova iz urbanih staništa i poređeni sa podacima dobijenim kod laboratorijskih sojeva pacova. Kod jedinki iz prirodnih populacija je zapažen veći opseg relativnih masa slezine, različite histomorfološke karakteristike i razlike u nivou i načinu odgovara ćelija slezine na egzogenu stimulaciju u poređenju sa jedinkama laboratorijskih sojeva. Ovi podaci ukazuju na kompleksne promene u imunskoj aktivnosti slezine kod jedinki iz prirodnih populacija.
T2  - Archives of Biological Sciences
T1  - Parametri imunske aktivnosti u slezini kod jedinki sivog pacova iz prirodnih populacija (Rattus norvegicus berkenhout, 1769) iz Srbije
T1  - Basic indices of spleen immune activity in natural populations of Norway rats (Rattus norvegicus berkenhout, 1769) in Serbia
IS  - 4
VL  - 61
SP  - 723
EP  - 732
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_239
ER  - 

@article{
author = "Kataranovski, Milena and Mirkov, Ivana and Zolotarevski, Lidija and Popov Aleksandrov, Aleksandra and Belij, Sandra and Stošić, Jelena and Kataranovski, D.",
year = "2009, 2009",
abstract = "Basic parameters of spleen immune activity (spleen weight, histomorphology of splenic compartments, and mitogen-induced splenocyte proliferative capacity in vitro) were evaluated in adult individuals of wild Norway rats from urban habitats and compared to the same data obtained in laboratory rat strains. A wider range of relative spleen mass and differential histomorphological characteristics, together with differences in the level and pattern of responsiveness of splenocytes to exogenous stimulation, were noted in spleens of wild Norway rats. Evidence of both enhanced and low-level immune-relevant spleen activity in wild rats demonstrates the complexity of changes in spleen immune activity in rats from natural populations., Ispitivani su osnovni parametri imunske aktivnosti u slezini (masa slezine, histomorfologija i sposobnost ćelija slezine da profilerišu u odgovoru na mitogen u in vitro uslovima) kod adultnih jedinki sivog pacova iz urbanih staništa i poređeni sa podacima dobijenim kod laboratorijskih sojeva pacova. Kod jedinki iz prirodnih populacija je zapažen veći opseg relativnih masa slezine, različite histomorfološke karakteristike i razlike u nivou i načinu odgovara ćelija slezine na egzogenu stimulaciju u poređenju sa jedinkama laboratorijskih sojeva. Ovi podaci ukazuju na kompleksne promene u imunskoj aktivnosti slezine kod jedinki iz prirodnih populacija.",
journal = "Archives of Biological Sciences",
title = "Parametri imunske aktivnosti u slezini kod jedinki sivog pacova iz prirodnih populacija (Rattus norvegicus berkenhout, 1769) iz Srbije, Basic indices of spleen immune activity in natural populations of Norway rats (Rattus norvegicus berkenhout, 1769) in Serbia",
number = "4",
volume = "61",
pages = "723-732",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_239"
}

Kataranovski, M., Mirkov, I., Zolotarevski, L., Popov Aleksandrov, A., Belij, S., Stošić, J.,& Kataranovski, D.. (2009). Parametri imunske aktivnosti u slezini kod jedinki sivog pacova iz prirodnih populacija (Rattus norvegicus berkenhout, 1769) iz Srbije. in Archives of Biological Sciences, 61(4), 723-732.
https://hdl.handle.net/21.15107/rcub_ibiss_239

Kataranovski M, Mirkov I, Zolotarevski L, Popov Aleksandrov A, Belij S, Stošić J, Kataranovski D. Parametri imunske aktivnosti u slezini kod jedinki sivog pacova iz prirodnih populacija (Rattus norvegicus berkenhout, 1769) iz Srbije. in Archives of Biological Sciences. 2009;61(4):723-732.
https://hdl.handle.net/21.15107/rcub_ibiss_239 .

Kataranovski, Milena, Mirkov, Ivana, Zolotarevski, Lidija, Popov Aleksandrov, Aleksandra, Belij, Sandra, Stošić, Jelena, Kataranovski, D., "Parametri imunske aktivnosti u slezini kod jedinki sivog pacova iz prirodnih populacija (Rattus norvegicus berkenhout, 1769) iz Srbije" in Archives of Biological Sciences, 61, no. 4 (2009):723-732,
https://hdl.handle.net/21.15107/rcub_ibiss_239 .