TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stupar, Sofija
AU  - Stanišić, Mariana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6710
AB  - Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.
PB  - Turkey. Gebze,Turkey: Technical University
C3  - Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
T1  - How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?
SP  - ID-739
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6710
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stupar, Sofija and Stanišić, Mariana",
year = "2024",
abstract = "Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.",
publisher = "Turkey. Gebze,Turkey: Technical University",
journal = "Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey",
title = "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?",
pages = "ID-739",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6710"
}

Smailagić, D., Dragišić Maksimović, J., Marin, M., Banjac, N., Ninković, S., Stupar, S.,& Stanišić, M.. (2024). How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
Turkey. Gebze,Turkey: Technical University., ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710

Smailagić D, Dragišić Maksimović J, Marin M, Banjac N, Ninković S, Stupar S, Stanišić M. How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey. 2024;:ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stupar, Sofija, Stanišić, Mariana, "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?" in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey (2024):ID-739,
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

TY  - CONF
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Ćosić, Tatjana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Motyka, Vaclav
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6112
AB  - Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling
SP  - 484
EP  - 484
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6112
ER  - 

@conference{
author = "Ninković, Slavica and Banjac, Nevena and Stanišić, Mariana and Smailagić, Dijana and Ćosić, Tatjana and Živanović, Branka and Dobrev, Petre I. and Motyka, Vaclav",
year = "2023",
abstract = "Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling",
pages = "484-484",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6112"
}

Ninković, S., Banjac, N., Stanišić, M., Smailagić, D., Ćosić, T., Živanović, B., Dobrev, P. I.,& Motyka, V.. (2023). Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112

Ninković S, Banjac N, Stanišić M, Smailagić D, Ćosić T, Živanović B, Dobrev PI, Motyka V. Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

Ninković, Slavica, Banjac, Nevena, Stanišić, Mariana, Smailagić, Dijana, Ćosić, Tatjana, Živanović, Branka, Dobrev, Petre I., Motyka, Vaclav, "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):484-484,
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Krstić-Milošević, Dijana
AU  - Mijalković, Tatjana
AU  - Petrović, Mirjana
AU  - Ćosić, Tatjana
AU  - Stanišić, Mariana
AU  - Vinterhalter, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5698
AB  - Eruca sativa is known in traditional medicine for its therapeutic effects, while young plants
are used as a salad or green food. Recently, the consumption of rocket has increased considerably,
so it has become very important for breeders. Plant tissue culture provides a platform to overcome
the problems in improving this species. In the present study, an efficient protocol for in vitro shoot
regeneration and propagation of recalcitrant rocket variety Domaća rukola was studied. Murashige
and Skoog (MS, 1962) medium containing 0.1 mg/L BA and frequent subculture over a period
of three weeks proved to be optimal for shoot multiplication with a multiplication index of over 3
and only 8.72% of hyperhydrated shoots without necrosis. Different concentrations of 2,4-D, BA, or
TDZ in combination with NAA, with or without the presence of AgNO3, were tested for de novo
shoot organogenesis (DNSO) from seedling explants. The hypocotyl explants cultured on MS with a
combination of TDZ1.0+NAA0.1+AgNO3 5.0 mg/L regenerated viable shoots with the highest rate
(25.38%) and an average number of 2.18 shoots per regenerating explant. Somatic embryogenesis
from immature zygotic embryos proved to be the best way to regenerate a recalcitrant rocket cultivar.
The highest embryogenic efficiency was achieved in explants cultured on MS medium containing
1.0 mg/L 2,4-D with a frequency of 76.64% and 5.13 mean number of regenerated somatic embryos
per explant, which were further converted into normal plants. Additionally, in vitro-produced rocket
shoots could serve as a possible promising source for the production of flavonoid kaempferol with
proven antioxidant properties.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola
IS  - 5
VL  - 9
DO  - 10.3390/horticulturae9050533
SP  - 533
ER  - 

@article{
author = "Banjac, Nevena and Krstić-Milošević, Dijana and Mijalković, Tatjana and Petrović, Mirjana and Ćosić, Tatjana and Stanišić, Mariana and Vinterhalter, Branka",
year = "2023",
abstract = "Eruca sativa is known in traditional medicine for its therapeutic effects, while young plants
are used as a salad or green food. Recently, the consumption of rocket has increased considerably,
so it has become very important for breeders. Plant tissue culture provides a platform to overcome
the problems in improving this species. In the present study, an efficient protocol for in vitro shoot
regeneration and propagation of recalcitrant rocket variety Domaća rukola was studied. Murashige
and Skoog (MS, 1962) medium containing 0.1 mg/L BA and frequent subculture over a period
of three weeks proved to be optimal for shoot multiplication with a multiplication index of over 3
and only 8.72% of hyperhydrated shoots without necrosis. Different concentrations of 2,4-D, BA, or
TDZ in combination with NAA, with or without the presence of AgNO3, were tested for de novo
shoot organogenesis (DNSO) from seedling explants. The hypocotyl explants cultured on MS with a
combination of TDZ1.0+NAA0.1+AgNO3 5.0 mg/L regenerated viable shoots with the highest rate
(25.38%) and an average number of 2.18 shoots per regenerating explant. Somatic embryogenesis
from immature zygotic embryos proved to be the best way to regenerate a recalcitrant rocket cultivar.
The highest embryogenic efficiency was achieved in explants cultured on MS medium containing
1.0 mg/L 2,4-D with a frequency of 76.64% and 5.13 mean number of regenerated somatic embryos
per explant, which were further converted into normal plants. Additionally, in vitro-produced rocket
shoots could serve as a possible promising source for the production of flavonoid kaempferol with
proven antioxidant properties.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola",
number = "5",
volume = "9",
doi = "10.3390/horticulturae9050533",
pages = "533"
}

Banjac, N., Krstić-Milošević, D., Mijalković, T., Petrović, M., Ćosić, T., Stanišić, M.,& Vinterhalter, B.. (2023). In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola. in Horticulturae
Basel: MDPI., 9(5), 533.
https://doi.org/10.3390/horticulturae9050533

Banjac N, Krstić-Milošević D, Mijalković T, Petrović M, Ćosić T, Stanišić M, Vinterhalter B. In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola. in Horticulturae. 2023;9(5):533.
doi:10.3390/horticulturae9050533 .

Banjac, Nevena, Krstić-Milošević, Dijana, Mijalković, Tatjana, Petrović, Mirjana, Ćosić, Tatjana, Stanišić, Mariana, Vinterhalter, Branka, "In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola" in Horticulturae, 9, no. 5 (2023):533,
https://doi.org/10.3390/horticulturae9050533 . .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Stupar, Sofija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5067
AB  - Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to 
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards 
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and 
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect 
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings 
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well 
as antioxidant enzymes activities were determined spectrophotometrically in treated and control 
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to 
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The 
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93% 
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced 
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin 
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities 
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of 
further investigation.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5067
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Stupar, Sofija and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to 
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards 
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and 
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect 
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings 
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well 
as antioxidant enzymes activities were determined spectrophotometrically in treated and control 
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to 
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The 
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93% 
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced 
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin 
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities 
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of 
further investigation.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5067"
}

Smailagić, D., Dragišić Maksimović, J., Stupar, S., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_5067

Smailagić D, Dragišić Maksimović J, Stupar S, Banjac N, Ninković S, Stanišić M. Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_5067 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Stupar, Sofija, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):73,
https://hdl.handle.net/21.15107/rcub_ibiss_5067 .

TY  - CONF
AU  - Bondokić, Jasna
AU  - Trajković, Milena
AU  - Dragišić Maksimović, Jelena
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5064
AB  - Phloretin is phenolic compound specifically produced in apple tree species and secreted into the soil as a response to biotic and abiotic factors. Since our previous results indicated roots as the primary site of phloretin phytotoxic action, this study focused on assessment of phloretin effects on the root growth of weed (Chenopodium murale and Lotus corniculatus),  crop (Solanum tuberosum) and woody species (Castanea sativa and Malus × domestica Borkh.). Significant decrease in root biomass production in liquid growth medium enriched with 500 µM phloretin was observed both after 2 and 4 weeks of treatment in all tested species. The percentages of roots growth inhibition were as follows: Chenopodium murale (95% and 89,1%), Lotus corniculatus (69,4% and 81,8%), Solanum tuberosum (88,4% and 84,7%), Castanea sativa (85,6% and 100%) and Malus × domestica Borkh. (65,3% and 87,4%, respectively). Total antioxidant capacity (TAC), total phenolic content (TPC), as well as antioxidant enzymes activities were determined spectrophotometrically in treated and control Chenopodium murale roots after 4 weeks of phloretin treatment. TAC increased 44,1% presumably due to elevated TPC (25,8%) in treated roots. However, there were no significant differences in catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) activities in control and treated roots after 4 weeks of treatment. Increased antioxidant activity in treated roots is presumably the consequence of known phloretin antioxidant potential.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5064
ER  - 

@conference{
author = "Bondokić, Jasna and Trajković, Milena and Dragišić Maksimović, Jelena and Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Phloretin is phenolic compound specifically produced in apple tree species and secreted into the soil as a response to biotic and abiotic factors. Since our previous results indicated roots as the primary site of phloretin phytotoxic action, this study focused on assessment of phloretin effects on the root growth of weed (Chenopodium murale and Lotus corniculatus),  crop (Solanum tuberosum) and woody species (Castanea sativa and Malus × domestica Borkh.). Significant decrease in root biomass production in liquid growth medium enriched with 500 µM phloretin was observed both after 2 and 4 weeks of treatment in all tested species. The percentages of roots growth inhibition were as follows: Chenopodium murale (95% and 89,1%), Lotus corniculatus (69,4% and 81,8%), Solanum tuberosum (88,4% and 84,7%), Castanea sativa (85,6% and 100%) and Malus × domestica Borkh. (65,3% and 87,4%, respectively). Total antioxidant capacity (TAC), total phenolic content (TPC), as well as antioxidant enzymes activities were determined spectrophotometrically in treated and control Chenopodium murale roots after 4 weeks of phloretin treatment. TAC increased 44,1% presumably due to elevated TPC (25,8%) in treated roots. However, there were no significant differences in catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) activities in control and treated roots after 4 weeks of treatment. Increased antioxidant activity in treated roots is presumably the consequence of known phloretin antioxidant potential.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5064"
}

Bondokić, J., Trajković, M., Dragišić Maksimović, J., Smailagić, D., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_5064

Bondokić J, Trajković M, Dragišić Maksimović J, Smailagić D, Banjac N, Ninković S, Stanišić M. The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_5064 .

Bondokić, Jasna, Trajković, Milena, Dragišić Maksimović, Jelena, Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):81,
https://hdl.handle.net/21.15107/rcub_ibiss_5064 .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5066
AB  - Phloretin is a phenolic compound unique for apple (Malus × domestica Borkh.) species. Our 
research group recently reported phytotoxic potential of phloretin towards other plant species 
such as model plant Arabidopsis. Since sugars in in vitro growth media could modulate plant 
metabolism, we analyzed phloretin effects on Arabidopsis seedlings grown on the medium with out sucrose and myoinositol (WSM) but enriched with different concentrations of phloretin (0 
to 1500 µM). Phloretin treatment on WSM induced seedlings growth retardation at much lower 
concentrations compared to sucrose and myoinositol enriched medium. Inhibition of primary 
root and leaf growth, and lateral root emergence increased with both phloretin concentration 
and treatment duration (maximal 85.2%, 30.4% and 46.5% at 1500 µM phloretin at 15 days after 
germination - DAG, respectively). Prolonged cultivation (4 weeks) at phloretin concentrations over 
500 µM induced complete growth stagnation and necrosis of the leaves. Transmission electron 
microscopy reviled relocation of chloroplasts within mesophyll cells, shape change into spherical 
form, as well as ultrastructural changes in thylakoid organization in leaves of 10 DAG-old seedlings 
treated with 250 or 500 µM phloretin. Significantly decreased number of starch grains in chloro plasts and chlorophyll a and b content in leaves reflected impaired function of chloroplasts due 
to ultrastructural changes. Phloretin also induced cell wall undulation and folds that resulted in 
irregularly shaped mesophyll cells. The presented study revealed that beside roots that had been 
previously emphasized as primary targets of phloretin action, leaves were also significantly affect ed by phloretin treatment, especially in the aspect of photosynthetic apparatus
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5066
ER  - 

@conference{
author = "Smailagić, Dijana and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Phloretin is a phenolic compound unique for apple (Malus × domestica Borkh.) species. Our 
research group recently reported phytotoxic potential of phloretin towards other plant species 
such as model plant Arabidopsis. Since sugars in in vitro growth media could modulate plant 
metabolism, we analyzed phloretin effects on Arabidopsis seedlings grown on the medium with out sucrose and myoinositol (WSM) but enriched with different concentrations of phloretin (0 
to 1500 µM). Phloretin treatment on WSM induced seedlings growth retardation at much lower 
concentrations compared to sucrose and myoinositol enriched medium. Inhibition of primary 
root and leaf growth, and lateral root emergence increased with both phloretin concentration 
and treatment duration (maximal 85.2%, 30.4% and 46.5% at 1500 µM phloretin at 15 days after 
germination - DAG, respectively). Prolonged cultivation (4 weeks) at phloretin concentrations over 
500 µM induced complete growth stagnation and necrosis of the leaves. Transmission electron 
microscopy reviled relocation of chloroplasts within mesophyll cells, shape change into spherical 
form, as well as ultrastructural changes in thylakoid organization in leaves of 10 DAG-old seedlings 
treated with 250 or 500 µM phloretin. Significantly decreased number of starch grains in chloro plasts and chlorophyll a and b content in leaves reflected impaired function of chloroplasts due 
to ultrastructural changes. Phloretin also induced cell wall undulation and folds that resulted in 
irregularly shaped mesophyll cells. The presented study revealed that beside roots that had been 
previously emphasized as primary targets of phloretin action, leaves were also significantly affect ed by phloretin treatment, especially in the aspect of photosynthetic apparatus",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5066"
}

Smailagić, D., Marin, M., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 72.
https://hdl.handle.net/21.15107/rcub_ibiss_5066

Smailagić D, Marin M, Banjac N, Ninković S, Stanišić M. Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:72.
https://hdl.handle.net/21.15107/rcub_ibiss_5066 .

Smailagić, Dijana, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):72,
https://hdl.handle.net/21.15107/rcub_ibiss_5066 .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Živanović, Branka
AU  - Maksimović, Vuk
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5063
AB  - Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5063
ER  - 

@conference{
author = "Stanišić, Mariana and Smailagić, Dijana and Živanović, Branka and Maksimović, Vuk and Ninković, Slavica and Banjac, Nevena",
year = "2022",
abstract = "Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings",
pages = "44",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5063"
}

Stanišić, M., Smailagić, D., Živanović, B., Maksimović, V., Ninković, S.,& Banjac, N.. (2022). What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063

Stanišić M, Smailagić D, Živanović B, Maksimović V, Ninković S, Banjac N. What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

Stanišić, Mariana, Smailagić, Dijana, Živanović, Branka, Maksimović, Vuk, Ninković, Slavica, Banjac, Nevena, "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):44,
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

TY  - CHAP
AU  - Stanišić, Mariana
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6567
AB  - Phloridzin and phloretin are dihydrochalcones highly specific to domestic apple (Malus × domestica
Borkh.) and other species from genus Malus. Auto-allelopathic potential of these phenolics was recognized in‘Apple Replant Disease’(ARD) characterized by stunted growth, reduced root system and low yields
in apple trees replanted on lands that previously supported apple orchards. Physiological basis of phloridzin and phloretin autotoxicity are ultrastructural and molecular alterations that include increased catalase, peroxidases and superoxide dismutase activities, raised malondialdehyde and proline contents, and
high expression of genes involved in cell defence system. The latest research on model plant Arabidopsis
thaliana indicates phytotoxicity of these dihydrochalcones towards other plant species. Phloretin inhibits
A. thaliana seedlings growth and development inducing agravitropic phenotype and morphological and
ultrastructural malformations in treated seedlings. Altered expression of CDKA1;1, CDKB2;1, CYCA3;1 and
CYCB2;4 cell cycle genes and genes involved in auxin homeostasis (PINs, AUX1, LAX3, ABCBs, TAA1 and YUCs)
and gibberellin biosynthesis (GA20ox2 and GA3ox1) are in the physiological bases of phloretin phytotoxicity. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves
the way for further research of phloretin role in ARD
AB  - Sekundarni metaboliti jabuke (Malus × domestica Borkh.) florizin i floretin su dihidrohalkoni visoko
specifični za vrste roda Malus. Autoalelopatski potencijal ovih fenolnih jedinjenja se ogleda u suzbijanju
rasta klijanca i sadnica zasađenih na mestu prvobitnih voćnjaka jabuke. U osnovi njihove fitotoksičnosti su
ultrastrukturne i molekularne promene koje se ogledaju u povećanju sadržaja malondialdehida, prolina i
aktivnosti antioksidativnih enzima: superoksid-dismutaze, peroksidaza i katalaza, kao i nivoa ekspresije
proteina uključenih u odbrambene mehanizme ćelije. Najnovija istraživanja ukazuju na fitotoksičnost ovih
dihidrohalkona i prema drugim biljnim vrstama, kao npr. korovskoj model biljci Arabidopsis thaliana. Tretman floretinom izaziva značajnu inhibiciju rastenja i razvića klijanaca A. thaliana, poremećaj gravitropskog
odgovora i pojavu morfoloških i ultrastrukturnih malformacija u čijoj osnovi leži promena ekspresije
CDKA1;1, CDKB2;1, CYCA3;1 i CYCB2;4 gena ćelijskog ciklusa, kao i gena uključenih u održanje homeostaze
auksina (PINs, AUX1, LAX3, ABCBs, TAA1 i YUCs) i biosinteze giberelina (GA20ox2 i GA3ox1). Ovo otvara
mogućnost za dalja istraživanja njihovog alelopatskog delovanja ali i potencijalnu primenu kao ekološki
bezbednih bioherbicida
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Trends in Molecular Biology
T1  - Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance
T1  - Apple dihydrochalcones phloridzin and phloretin as novel allelochemicals
IS  - 2
SP  - 223
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6567
ER  - 

@inbook{
author = "Stanišić, Mariana and Ninković, Slavica and Banjac, Nevena",
year = "2022",
abstract = "Phloridzin and phloretin are dihydrochalcones highly specific to domestic apple (Malus × domestica
Borkh.) and other species from genus Malus. Auto-allelopathic potential of these phenolics was recognized in‘Apple Replant Disease’(ARD) characterized by stunted growth, reduced root system and low yields
in apple trees replanted on lands that previously supported apple orchards. Physiological basis of phloridzin and phloretin autotoxicity are ultrastructural and molecular alterations that include increased catalase, peroxidases and superoxide dismutase activities, raised malondialdehyde and proline contents, and
high expression of genes involved in cell defence system. The latest research on model plant Arabidopsis
thaliana indicates phytotoxicity of these dihydrochalcones towards other plant species. Phloretin inhibits
A. thaliana seedlings growth and development inducing agravitropic phenotype and morphological and
ultrastructural malformations in treated seedlings. Altered expression of CDKA1;1, CDKB2;1, CYCA3;1 and
CYCB2;4 cell cycle genes and genes involved in auxin homeostasis (PINs, AUX1, LAX3, ABCBs, TAA1 and YUCs)
and gibberellin biosynthesis (GA20ox2 and GA3ox1) are in the physiological bases of phloretin phytotoxicity. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves
the way for further research of phloretin role in ARD, Sekundarni metaboliti jabuke (Malus × domestica Borkh.) florizin i floretin su dihidrohalkoni visoko
specifični za vrste roda Malus. Autoalelopatski potencijal ovih fenolnih jedinjenja se ogleda u suzbijanju
rasta klijanca i sadnica zasađenih na mestu prvobitnih voćnjaka jabuke. U osnovi njihove fitotoksičnosti su
ultrastrukturne i molekularne promene koje se ogledaju u povećanju sadržaja malondialdehida, prolina i
aktivnosti antioksidativnih enzima: superoksid-dismutaze, peroksidaza i katalaza, kao i nivoa ekspresije
proteina uključenih u odbrambene mehanizme ćelije. Najnovija istraživanja ukazuju na fitotoksičnost ovih
dihidrohalkona i prema drugim biljnim vrstama, kao npr. korovskoj model biljci Arabidopsis thaliana. Tretman floretinom izaziva značajnu inhibiciju rastenja i razvića klijanaca A. thaliana, poremećaj gravitropskog
odgovora i pojavu morfoloških i ultrastrukturnih malformacija u čijoj osnovi leži promena ekspresije
CDKA1;1, CDKB2;1, CYCA3;1 i CYCB2;4 gena ćelijskog ciklusa, kao i gena uključenih u održanje homeostaze
auksina (PINs, AUX1, LAX3, ABCBs, TAA1 i YUCs) i biosinteze giberelina (GA20ox2 i GA3ox1). Ovo otvara
mogućnost za dalja istraživanja njihovog alelopatskog delovanja ali i potencijalnu primenu kao ekološki
bezbednih bioherbicida",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Trends in Molecular Biology",
booktitle = "Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance, Apple dihydrochalcones phloridzin and phloretin as novel allelochemicals",
number = "2",
pages = "223-232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6567"
}

Stanišić, M., Ninković, S.,& Banjac, N.. (2022). Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance. in Trends in Molecular Biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(2), 223-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6567

Stanišić M, Ninković S, Banjac N. Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance. in Trends in Molecular Biology. 2022;(2):223-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6567 .

Stanišić, Mariana, Ninković, Slavica, Banjac, Nevena, "Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance" in Trends in Molecular Biology, no. 2 (2022):223-232,
https://hdl.handle.net/21.15107/rcub_ibiss_6567 .

TY  - JOUR
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Pěnčík, Aleš
AU  - Ćalić, Dušica
AU  - Bogdanović, Milica
AU  - Trajković, Milena
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5065
AB  - Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action
VL  - 13
DO  - 0.3389/fpls.2022.875528
SP  - 875528
ER  - 

@article{
author = "Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Pěnčík, Aleš and Ćalić, Dušica and Bogdanović, Milica and Trajković, Milena and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action",
volume = "13",
doi = "0.3389/fpls.2022.875528",
pages = "875528"
}

Smailagić, D., Banjac, N., Ninković, S., Savić, J., Ćosić, T., Pěnčík, A., Ćalić, D., Bogdanović, M., Trajković, M.,& Stanišić, M.. (2022). New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 13, 875528.
https://doi.org/0.3389/fpls.2022.875528

Smailagić D, Banjac N, Ninković S, Savić J, Ćosić T, Pěnčík A, Ćalić D, Bogdanović M, Trajković M, Stanišić M. New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science. 2022;13:875528.
doi:0.3389/fpls.2022.875528 .

Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Pěnčík, Aleš, Ćalić, Dušica, Bogdanović, Milica, Trajković, Milena, Stanišić, Mariana, "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action" in Frontiers in Plant Science, 13 (2022):875528,
https://doi.org/0.3389/fpls.2022.875528 . .

TY  - CONF
AU  - Vinterhalter, Branka
AU  - Krstić-Milošević, Dijana
AU  - Petrović, Mirjana
AU  - Devrnja, Nina
AU  - Banjac, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5754
AB  - Eruca sativa (rocket, Brassicaceae), is known in traditional medicine for its therapeutic, while young plants are used as salad, vegetable, or green foods. Genetic improvement of the rocket has been limited due to the appearance of biennial plants and the requirement for isolation barriers. Plant tissue culture offers a platform to overcome existing barriers to improving this species. The present study aimed to achieve a feasible protocol for shoot regeneration and propagation of rocket cv. Domaća rukola and to evaluate the use of AgNO3 to improve regeneration efficiency. Proliferation of shoots from seedling epicotyl, shoot organogenesis from root, hypocotyl and cotyledon seedling explants, and somatic embryogenesis from immature zygotic embryos were studied. Murashige and Skoog (MS) basal medium in combination with a low KIN concentration of 0.1 mg l-1 with frequent subculture over a period of 3 weeks was found to be optimal for shoot multiplication with a multiplication index of almost 3 and only 9.3% of vitrified shoots without necrosis. Different concentrations of 2,4-D, BA or TDZ in combination with NAA and with or without AgNO3 were tested for shoot regeneration from seedling explants. As a result, the hypocotyl explants cultured on MS with a combination of TDZ 1.0+NAA 0.1+AgNO3 5.0 mg l-1 provided healthy shoots with a satisfactory regeneration rate of 25.4% and 2.2 mean number of shoots per regenerating explant. Immature zygotic embryos cultured on MS medium containing 1.0 mg l-1 2.4-D showed the highest regeneration frequency (78.76%) as well as the number of regenerated somatic embryos per explant (5.13) with low incidence of callusing and necrosis. KIN at 0.5 mg l-1 enabled the best conversion of somatic embryos into healthy plants (22.5%). The results provide an opportunity to use established regeneration protocols in rocket breeding improvement programs.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
T1  - In vitro shoot propagation and regeneration of rocket (Eruca sativa Mill.) using organogenesis and somatic embryogenesis
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5754
ER  - 

@conference{
author = "Vinterhalter, Branka and Krstić-Milošević, Dijana and Petrović, Mirjana and Devrnja, Nina and Banjac, Nevena",
year = "2022",
abstract = "Eruca sativa (rocket, Brassicaceae), is known in traditional medicine for its therapeutic, while young plants are used as salad, vegetable, or green foods. Genetic improvement of the rocket has been limited due to the appearance of biennial plants and the requirement for isolation barriers. Plant tissue culture offers a platform to overcome existing barriers to improving this species. The present study aimed to achieve a feasible protocol for shoot regeneration and propagation of rocket cv. Domaća rukola and to evaluate the use of AgNO3 to improve regeneration efficiency. Proliferation of shoots from seedling epicotyl, shoot organogenesis from root, hypocotyl and cotyledon seedling explants, and somatic embryogenesis from immature zygotic embryos were studied. Murashige and Skoog (MS) basal medium in combination with a low KIN concentration of 0.1 mg l-1 with frequent subculture over a period of 3 weeks was found to be optimal for shoot multiplication with a multiplication index of almost 3 and only 9.3% of vitrified shoots without necrosis. Different concentrations of 2,4-D, BA or TDZ in combination with NAA and with or without AgNO3 were tested for shoot regeneration from seedling explants. As a result, the hypocotyl explants cultured on MS with a combination of TDZ 1.0+NAA 0.1+AgNO3 5.0 mg l-1 provided healthy shoots with a satisfactory regeneration rate of 25.4% and 2.2 mean number of shoots per regenerating explant. Immature zygotic embryos cultured on MS medium containing 1.0 mg l-1 2.4-D showed the highest regeneration frequency (78.76%) as well as the number of regenerated somatic embryos per explant (5.13) with low incidence of callusing and necrosis. KIN at 0.5 mg l-1 enabled the best conversion of somatic embryos into healthy plants (22.5%). The results provide an opportunity to use established regeneration protocols in rocket breeding improvement programs.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia",
title = "In vitro shoot propagation and regeneration of rocket (Eruca sativa Mill.) using organogenesis and somatic embryogenesis",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5754"
}

Vinterhalter, B., Krstić-Milošević, D., Petrović, M., Devrnja, N.,& Banjac, N.. (2022). In vitro shoot propagation and regeneration of rocket (Eruca sativa Mill.) using organogenesis and somatic embryogenesis. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
Ljubljana: Slovenian Society of Plant Biology., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_5754

Vinterhalter B, Krstić-Milošević D, Petrović M, Devrnja N, Banjac N. In vitro shoot propagation and regeneration of rocket (Eruca sativa Mill.) using organogenesis and somatic embryogenesis. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. 2022;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_5754 .

Vinterhalter, Branka, Krstić-Milošević, Dijana, Petrović, Mirjana, Devrnja, Nina, Banjac, Nevena, "In vitro shoot propagation and regeneration of rocket (Eruca sativa Mill.) using organogenesis and somatic embryogenesis" in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia (2022):18,
https://hdl.handle.net/21.15107/rcub_ibiss_5754 .

TY  - CONF
AU  - Krstić-Milošević, Dijana
AU  - Banjac, Nevena
AU  - Janković, Teodora
AU  - Devrnja, Nina
AU  - Eler, Klemen
AU  - Vinterhalter, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5560
AB  - Gentiana clusii Perr. & Song. (Gentianaceae), is a rare plant species found in the Alps, the Pyrenees, the Black Forest Mountains and the Carpathians. G. clusii is endemic for Europe which implies the need for its conservation. G. clusii plants were collected at fruitful stage in June 2015 at Velika Planina Mountain, Republic of Slovenia. Seeds of G. clusii were germinated on MS basal medium. Shoots cultured on WPM + 0.5 mg/L KIN were used as starting material in propagation and rooting experiments. Medium with IBA at 0.5 mg/L was chosen as optimal for rooting. HPLC screening of secondary metabolites of wild plants collected in nature and in vitro plants revealed the presence of two main classes of compounds typical for gentians – secoiridoids and xanthones. Secoiridoids gentiopicrin and sweroside were identified in both aerial parts of wild plants and shoots of in vitro plants, while root extracts contained amarogentin in addition. Aerial parts of wild plants contained gentiacaulein-1-O-primeveroside as dominant xanthone glycoside. HPLC also revealed the presence of gentiakochianin-1-O-primeveroside, norswertianin-1-O-primeveroside, 1,8- dihydroxy-3-methoxy-7-O-primeverosyl xanthone, and xanthone aglycons- norswertianin, gentiacaulein, and gentiakochianin. HPLC analysis shown that in vitro culture considerably promoted the production of secondary metabolites in G. clusii. The selected protocol for shoot propagation (WPM + 0.5 mg/L KIN) increased the content of sweroside, gentiopicrin and norswertianin-1-O-primeveroside for more than 2-fold compared with the wild plants. IBA promoted norswertianin-1-O-primeveroside and norswertianin production in root cultures; their contents were enhanced 6.4- and 18.6-fold, respectively, compared with the wild plants. The extract of these roots displayed high antioxidant capacity. The established shoot and root propagation protocols facilitate in vitro conservation of G. clusii, and provides a promising tool for the large scale production of valuable secoiridoids and xanthones.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
T1  - Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5560
ER  - 

@conference{
editor = "Baebler, Špela, Dermastia, Marina, Grebenc, Tine, Praprotnik, Eva, Razinger, Jaka, Urbanek Krajnc, Andreja",
author = "Krstić-Milošević, Dijana and Banjac, Nevena and Janković, Teodora and Devrnja, Nina and Eler, Klemen and Vinterhalter, Branka",
year = "2022",
abstract = "Gentiana clusii Perr. & Song. (Gentianaceae), is a rare plant species found in the Alps, the Pyrenees, the Black Forest Mountains and the Carpathians. G. clusii is endemic for Europe which implies the need for its conservation. G. clusii plants were collected at fruitful stage in June 2015 at Velika Planina Mountain, Republic of Slovenia. Seeds of G. clusii were germinated on MS basal medium. Shoots cultured on WPM + 0.5 mg/L KIN were used as starting material in propagation and rooting experiments. Medium with IBA at 0.5 mg/L was chosen as optimal for rooting. HPLC screening of secondary metabolites of wild plants collected in nature and in vitro plants revealed the presence of two main classes of compounds typical for gentians – secoiridoids and xanthones. Secoiridoids gentiopicrin and sweroside were identified in both aerial parts of wild plants and shoots of in vitro plants, while root extracts contained amarogentin in addition. Aerial parts of wild plants contained gentiacaulein-1-O-primeveroside as dominant xanthone glycoside. HPLC also revealed the presence of gentiakochianin-1-O-primeveroside, norswertianin-1-O-primeveroside, 1,8- dihydroxy-3-methoxy-7-O-primeverosyl xanthone, and xanthone aglycons- norswertianin, gentiacaulein, and gentiakochianin. HPLC analysis shown that in vitro culture considerably promoted the production of secondary metabolites in G. clusii. The selected protocol for shoot propagation (WPM + 0.5 mg/L KIN) increased the content of sweroside, gentiopicrin and norswertianin-1-O-primeveroside for more than 2-fold compared with the wild plants. IBA promoted norswertianin-1-O-primeveroside and norswertianin production in root cultures; their contents were enhanced 6.4- and 18.6-fold, respectively, compared with the wild plants. The extract of these roots displayed high antioxidant capacity. The established shoot and root propagation protocols facilitate in vitro conservation of G. clusii, and provides a promising tool for the large scale production of valuable secoiridoids and xanthones.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia",
title = "Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity",
pages = "31",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5560"
}

Baebler, Š., Dermastia, M., Grebenc, T., Praprotnik, E., Razinger, J., Urbanek Krajnc, A., Krstić-Milošević, D., Banjac, N., Janković, T., Devrnja, N., Eler, K.,& Vinterhalter, B.. (2022). Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
Ljubljana: Slovenian Society of Plant Biology., 31.
https://hdl.handle.net/21.15107/rcub_ibiss_5560

Baebler Š, Dermastia M, Grebenc T, Praprotnik E, Razinger J, Urbanek Krajnc A, Krstić-Milošević D, Banjac N, Janković T, Devrnja N, Eler K, Vinterhalter B. Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. 2022;:31.
https://hdl.handle.net/21.15107/rcub_ibiss_5560 .

Baebler, Špela, Dermastia, Marina, Grebenc, Tine, Praprotnik, Eva, Razinger, Jaka, Urbanek Krajnc, Andreja, Krstić-Milošević, Dijana, Banjac, Nevena, Janković, Teodora, Devrnja, Nina, Eler, Klemen, Vinterhalter, Branka, "Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity" in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia (2022):31,
https://hdl.handle.net/21.15107/rcub_ibiss_5560 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Stanisavljević, Rade
AU  - Dimkić, Ivica
AU  - Velijević, Nataša
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4173
AB  - Potential of Trichoderma harzianum IS005-12 (TH-IS005-12) to promote seed germination and seedling growth of Italian ryegrass (IRG) forage was evaluated in vitro. Non-desiccated seeds and those pre-harvestly desiccated with total herbicide were treated 25 days (freshly harvested) and 178 days after harvest (mature) with TH-IS005-12 spore suspensions at 0 (T0), 1.8 × 107 (T1) and 1.2 × 109 (T2) spore/mL. TH-IS005-12 promoted the early and final germination and seedling growth in all non-desiccated and desiccated, freshly harvested as well as mature IRG seeds. It was more effective in pre-harvestly desiccated freshly harvested seeds where T2 treatment increased final germination rate for 24%, root number per seedling 1.6-fold and seedling vigour 1.9-fold compared to the untreated control. Moreover, TH-IS005-12 showed an inhibitory activity against seedborne fungi Alternaria alternata and A. ventricosa suppressing their growth in vitro by 82% and 77%, respectively.
PB  - Czech Academy of Agricultural Sciences
T2  - Plant, Soil and Environment
T2  - Plant, Soil and Environment
T1  - Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage
IS  - 3
VL  - 67
DO  - 10.17221/581/2020-pse
SP  - 130
EP  - 136
ER  - 

@article{
author = "Banjac, Nevena and Stanisavljević, Rade and Dimkić, Ivica and Velijević, Nataša and Soković, Marina and Ćirić, Ana",
year = "2021",
abstract = "Potential of Trichoderma harzianum IS005-12 (TH-IS005-12) to promote seed germination and seedling growth of Italian ryegrass (IRG) forage was evaluated in vitro. Non-desiccated seeds and those pre-harvestly desiccated with total herbicide were treated 25 days (freshly harvested) and 178 days after harvest (mature) with TH-IS005-12 spore suspensions at 0 (T0), 1.8 × 107 (T1) and 1.2 × 109 (T2) spore/mL. TH-IS005-12 promoted the early and final germination and seedling growth in all non-desiccated and desiccated, freshly harvested as well as mature IRG seeds. It was more effective in pre-harvestly desiccated freshly harvested seeds where T2 treatment increased final germination rate for 24%, root number per seedling 1.6-fold and seedling vigour 1.9-fold compared to the untreated control. Moreover, TH-IS005-12 showed an inhibitory activity against seedborne fungi Alternaria alternata and A. ventricosa suppressing their growth in vitro by 82% and 77%, respectively.",
publisher = "Czech Academy of Agricultural Sciences",
journal = "Plant, Soil and Environment, Plant, Soil and Environment",
title = "Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage",
number = "3",
volume = "67",
doi = "10.17221/581/2020-pse",
pages = "130-136"
}

Banjac, N., Stanisavljević, R., Dimkić, I., Velijević, N., Soković, M.,& Ćirić, A.. (2021). Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage. in Plant, Soil and Environment
Czech Academy of Agricultural Sciences., 67(3), 130-136.
https://doi.org/10.17221/581/2020-pse

Banjac N, Stanisavljević R, Dimkić I, Velijević N, Soković M, Ćirić A. Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage. in Plant, Soil and Environment. 2021;67(3):130-136.
doi:10.17221/581/2020-pse .

Banjac, Nevena, Stanisavljević, Rade, Dimkić, Ivica, Velijević, Nataša, Soković, Marina, Ćirić, Ana, "Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage" in Plant, Soil and Environment, 67, no. 3 (2021):130-136,
https://doi.org/10.17221/581/2020-pse . .

TY  - JOUR
AU  - Krstić Milošević, Dijana
AU  - Banjac, Nevena
AU  - Janković, Teodora
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4310
AB  - In vitro shoot culture of the endangered medicinal plant Gentianella lutescens was established from epicotyl explants cultured on MS basal medium with 0.2 mg L−1 6-benzylaminopurine (BA) and evaluated for xanthones content for the first time. Five shoot lines were obtained and no significant variations in multiplication rate, shoot elongation, and xanthones profile were found among them. The highest rooting rate (33.3%) was achieved by shoots treated for 2 days with 5 mg L−1 indole-3-butyric acid (IBA) followed by cultivation in liquid PGR-free ½ MS medium for 60 days. HPLC analysis revealed the lower content of xanthones—mangiferin, bellidifolin, demethylbellidifolin, demethylbellidifolin-8-O-glucoside and bellidifolin-8-O-glucoside—in in vitro cultured shoots compared to wild growing plants. The increasing concentration of sucrose, sorbitol and abiotic elicitors salicylic acid (SA), jasmonic acid (JA) and methyl jasmonate (MeJA) altered shoot growth and xanthone production. Sucrose and sorbitol applied at the highest concentration of 233.6 mM increased dry matter percentage, while SA at 100 μM promoted shoot growth 2-fold. The increased sucrose concentration enhanced accumulation of xanthones in shoot cultures 2–3-fold compared to the control shoots. Elicitors at 100–300 μM increased the accumulation of mangiferin, demethylbellidifolin-8-O-glucoside, and bellidifolin-8-O-glucoside almost equally, while MeJA at the highest concentration of 500 μM enhanced amount of aglycones demethylbellidifolin and bellidifolin 7-fold compared to the control. The obtained results facilitate conservation of G. lutescens and pave the way for further research on large-scale shoot propagation and production of pharmacologically active xanthones.
PB  - Basel: MDPI
T2  - Plants
T1  - Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production
IS  - 8
VL  - 10
DO  - 10.3390/plants10081651
SP  - 1651
ER  - 

@article{
author = "Krstić Milošević, Dijana and Banjac, Nevena and Janković, Teodora and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2021",
abstract = "In vitro shoot culture of the endangered medicinal plant Gentianella lutescens was established from epicotyl explants cultured on MS basal medium with 0.2 mg L−1 6-benzylaminopurine (BA) and evaluated for xanthones content for the first time. Five shoot lines were obtained and no significant variations in multiplication rate, shoot elongation, and xanthones profile were found among them. The highest rooting rate (33.3%) was achieved by shoots treated for 2 days with 5 mg L−1 indole-3-butyric acid (IBA) followed by cultivation in liquid PGR-free ½ MS medium for 60 days. HPLC analysis revealed the lower content of xanthones—mangiferin, bellidifolin, demethylbellidifolin, demethylbellidifolin-8-O-glucoside and bellidifolin-8-O-glucoside—in in vitro cultured shoots compared to wild growing plants. The increasing concentration of sucrose, sorbitol and abiotic elicitors salicylic acid (SA), jasmonic acid (JA) and methyl jasmonate (MeJA) altered shoot growth and xanthone production. Sucrose and sorbitol applied at the highest concentration of 233.6 mM increased dry matter percentage, while SA at 100 μM promoted shoot growth 2-fold. The increased sucrose concentration enhanced accumulation of xanthones in shoot cultures 2–3-fold compared to the control shoots. Elicitors at 100–300 μM increased the accumulation of mangiferin, demethylbellidifolin-8-O-glucoside, and bellidifolin-8-O-glucoside almost equally, while MeJA at the highest concentration of 500 μM enhanced amount of aglycones demethylbellidifolin and bellidifolin 7-fold compared to the control. The obtained results facilitate conservation of G. lutescens and pave the way for further research on large-scale shoot propagation and production of pharmacologically active xanthones.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production",
number = "8",
volume = "10",
doi = "10.3390/plants10081651",
pages = "1651"
}

Krstić Milošević, D., Banjac, N., Janković, T., Vinterhalter, D.,& Vinterhalter, B.. (2021). Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production. in Plants
Basel: MDPI., 10(8), 1651.
https://doi.org/10.3390/plants10081651

Krstić Milošević D, Banjac N, Janković T, Vinterhalter D, Vinterhalter B. Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production. in Plants. 2021;10(8):1651.
doi:10.3390/plants10081651 .

Krstić Milošević, Dijana, Banjac, Nevena, Janković, Teodora, Vinterhalter, Dragan, Vinterhalter, Branka, "Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production" in Plants, 10, no. 8 (2021):1651,
https://doi.org/10.3390/plants10081651 . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Banjac, Nevena
AU  - Vinterhalter, Dragan
AU  - Krstić Milošević, Dijana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4309
AB  - The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.
PB  - Basel: MDPI
T2  - Plants
T1  - Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors
IS  - 8
VL  - 10
DO  - 10.3390/plants10081610
SP  - 1610
ER  - 

@article{
author = "Vinterhalter, Branka and Banjac, Nevena and Vinterhalter, Dragan and Krstić Milošević, Dijana",
year = "2021",
abstract = "The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors",
number = "8",
volume = "10",
doi = "10.3390/plants10081610",
pages = "1610"
}

Vinterhalter, B., Banjac, N., Vinterhalter, D.,& Krstić Milošević, D.. (2021). Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors. in Plants
Basel: MDPI., 10(8), 1610.
https://doi.org/10.3390/plants10081610

Vinterhalter B, Banjac N, Vinterhalter D, Krstić Milošević D. Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors. in Plants. 2021;10(8):1610.
doi:10.3390/plants10081610 .

Vinterhalter, Branka, Banjac, Nevena, Vinterhalter, Dragan, Krstić Milošević, Dijana, "Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors" in Plants, 10, no. 8 (2021):1610,
https://doi.org/10.3390/plants10081610 . .

TY  - JOUR
AU  - Poštić, Dobrivoje
AU  - Štrbanović, Ratibor
AU  - Tabaković, Marijenka
AU  - Popović, Tatjana
AU  - Ćirić, Ana
AU  - Banjac, Nevena
AU  - Trkulja, Nenad
AU  - Stanisavljević, Rade
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4401
AB  - Seed treatments with zinc, boron, biostimulant Coveron and MIX (zinc + boron + Coveron)
were applied to three lettuce and three celeriac cultivars. Seeds of three wheat cultivars were treated
under laboratory conditions with Trichoderma harzianum and eight Bacillus spp. Seed germination,
seedling growth, and the presence of the following pathogens were determined: Fusarium sp., Al‐
ternaria sp., Penicillium sp., and Mucor sp. The Coveron treatment was the most effective on lettuce
seeds tested in the germination cabinet. Seed germination was higher by 4% than in the control.
Alternatively, germination of seeds treated with boron in the greenhouse was higher by 12% than
in the control. The Coveron treatment had the highest effect on the shoot length, which was greater
by 0.7 and 2.1 cm in the germination cabinet and the greenhouse, respectively. This treatment was
also the most effective on the root length. Zn, B, and MIX treatments increased celeriac seed germi‐
nation by 14% in the germination cabinet. The Zn treatment was the most efficient on seeds tested
in the greenhouse. The germination was higher by 15%. A significant cultivar × treatment interac‐
tion was determined in both observed species under both conditions. The maximum effect on wheat
seed germination (8%) was achieved with the T. harzianum treatment in the Salazar cultivar. A sig‐
nificant interdependence (p ≤ 0.01 to p ≤ 0.001) was established between seed germination and the
seedling growth. The interrelationship between seed germination and pathogens of all cultivars was
negative.
PB  - Basel: MDPI
T2  - Plants
T1  - Germination and the Initial Seedling Growth of Lettuce, Celeriac and Wheat Cultivars after Micronutrient and a Biological Application Pre-Sowing Seed Treatment
IS  - 9
VL  - 10
DO  - 10.3390/plants10091913
SP  - 1913
ER  - 

@article{
author = "Poštić, Dobrivoje and Štrbanović, Ratibor and Tabaković, Marijenka and Popović, Tatjana and Ćirić, Ana and Banjac, Nevena and Trkulja, Nenad and Stanisavljević, Rade",
year = "2021",
abstract = "Seed treatments with zinc, boron, biostimulant Coveron and MIX (zinc + boron + Coveron)
were applied to three lettuce and three celeriac cultivars. Seeds of three wheat cultivars were treated
under laboratory conditions with Trichoderma harzianum and eight Bacillus spp. Seed germination,
seedling growth, and the presence of the following pathogens were determined: Fusarium sp., Al‐
ternaria sp., Penicillium sp., and Mucor sp. The Coveron treatment was the most effective on lettuce
seeds tested in the germination cabinet. Seed germination was higher by 4% than in the control.
Alternatively, germination of seeds treated with boron in the greenhouse was higher by 12% than
in the control. The Coveron treatment had the highest effect on the shoot length, which was greater
by 0.7 and 2.1 cm in the germination cabinet and the greenhouse, respectively. This treatment was
also the most effective on the root length. Zn, B, and MIX treatments increased celeriac seed germi‐
nation by 14% in the germination cabinet. The Zn treatment was the most efficient on seeds tested
in the greenhouse. The germination was higher by 15%. A significant cultivar × treatment interac‐
tion was determined in both observed species under both conditions. The maximum effect on wheat
seed germination (8%) was achieved with the T. harzianum treatment in the Salazar cultivar. A sig‐
nificant interdependence (p ≤ 0.01 to p ≤ 0.001) was established between seed germination and the
seedling growth. The interrelationship between seed germination and pathogens of all cultivars was
negative.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Germination and the Initial Seedling Growth of Lettuce, Celeriac and Wheat Cultivars after Micronutrient and a Biological Application Pre-Sowing Seed Treatment",
number = "9",
volume = "10",
doi = "10.3390/plants10091913",
pages = "1913"
}

Poštić, D., Štrbanović, R., Tabaković, M., Popović, T., Ćirić, A., Banjac, N., Trkulja, N.,& Stanisavljević, R.. (2021). Germination and the Initial Seedling Growth of Lettuce, Celeriac and Wheat Cultivars after Micronutrient and a Biological Application Pre-Sowing Seed Treatment. in Plants
Basel: MDPI., 10(9), 1913.
https://doi.org/10.3390/plants10091913

Poštić D, Štrbanović R, Tabaković M, Popović T, Ćirić A, Banjac N, Trkulja N, Stanisavljević R. Germination and the Initial Seedling Growth of Lettuce, Celeriac and Wheat Cultivars after Micronutrient and a Biological Application Pre-Sowing Seed Treatment. in Plants. 2021;10(9):1913.
doi:10.3390/plants10091913 .

Poštić, Dobrivoje, Štrbanović, Ratibor, Tabaković, Marijenka, Popović, Tatjana, Ćirić, Ana, Banjac, Nevena, Trkulja, Nenad, Stanisavljević, Rade, "Germination and the Initial Seedling Growth of Lettuce, Celeriac and Wheat Cultivars after Micronutrient and a Biological Application Pre-Sowing Seed Treatment" in Plants, 10, no. 9 (2021):1913,
https://doi.org/10.3390/plants10091913 . .

TY  - JOUR
AU  - Krstić Milošević, Dijana
AU  - Banjac, Nevena
AU  - Janković, Teodora
AU  - Eler, Klemen
AU  - Vinterhalter, Branka
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32763798
UR  - https://radar.ibiss.bg.ac.rs/123456789/3847
AB  - Shoot and root in vitro culture of endemic European species Gentiana clusii was established for the first time. The effects of different concentrations of benzyl adenine (BA), 6-phurphurylaminopurine (KIN), indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) on shoot propagation and rooting of G. clusii were investigated. The optimal in vitro conditions for shoot propagation and long-term maintenance were achieved using woody plant medium (WPM) supplemented with 0.5 mg l-1 KIN, and subsequent application of IBA at 0.5 mg l-1 significantly improved rooting of these shoots. Root culture was established from excised root tips cultured in ½ MS liquid media with increasing concentrations of IBA (0.1-1.0 mg l-1). A high root growth rate and considerable biomass yield were obtained by addition of 1.0 mg l-1 IBA. HPLC analysis revealed that in vitro culture considerably promoted the production of secondary metabolites in G. clusii. The selected protocol for shoot propagation (WPM + 0.5 mg l-1 KIN) increased the content of sweroside, gentiopicrin and norswertianin-1-O-primeveroside (N-1-P) for more than 2-fold compared with the wild plants. IBA promoted N-1-P and norswertianin production in root cultures; their contents were enhanced 6.4- and 18.6-fold, respectively, compared with the wild plants. The extract of these roots displayed the highest antioxidant capacity (IC50 = 66.57 μg ml-1). The established shoot and root propagation protocols facilitate in vitro conservation of G. clusii, and provides a promising tool for the large scale production of valuable secoiridoids and xanthones.
PB  - Elsevier Masson SAS
T2  - Plant Physiology and Biochemistry
T1  - Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation.
VL  - 154
DO  - 10.1016/j.plaphy.2020.07.007
SP  - 735
EP  - 744
ER  - 

@article{
author = "Krstić Milošević, Dijana and Banjac, Nevena and Janković, Teodora and Eler, Klemen and Vinterhalter, Branka",
year = "2020",
abstract = "Shoot and root in vitro culture of endemic European species Gentiana clusii was established for the first time. The effects of different concentrations of benzyl adenine (BA), 6-phurphurylaminopurine (KIN), indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) on shoot propagation and rooting of G. clusii were investigated. The optimal in vitro conditions for shoot propagation and long-term maintenance were achieved using woody plant medium (WPM) supplemented with 0.5 mg l-1 KIN, and subsequent application of IBA at 0.5 mg l-1 significantly improved rooting of these shoots. Root culture was established from excised root tips cultured in ½ MS liquid media with increasing concentrations of IBA (0.1-1.0 mg l-1). A high root growth rate and considerable biomass yield were obtained by addition of 1.0 mg l-1 IBA. HPLC analysis revealed that in vitro culture considerably promoted the production of secondary metabolites in G. clusii. The selected protocol for shoot propagation (WPM + 0.5 mg l-1 KIN) increased the content of sweroside, gentiopicrin and norswertianin-1-O-primeveroside (N-1-P) for more than 2-fold compared with the wild plants. IBA promoted N-1-P and norswertianin production in root cultures; their contents were enhanced 6.4- and 18.6-fold, respectively, compared with the wild plants. The extract of these roots displayed the highest antioxidant capacity (IC50 = 66.57 μg ml-1). The established shoot and root propagation protocols facilitate in vitro conservation of G. clusii, and provides a promising tool for the large scale production of valuable secoiridoids and xanthones.",
publisher = "Elsevier Masson SAS",
journal = "Plant Physiology and Biochemistry",
title = "Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation.",
volume = "154",
doi = "10.1016/j.plaphy.2020.07.007",
pages = "735-744"
}

Krstić Milošević, D., Banjac, N., Janković, T., Eler, K.,& Vinterhalter, B.. (2020). Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation.. in Plant Physiology and Biochemistry
Elsevier Masson SAS., 154, 735-744.
https://doi.org/10.1016/j.plaphy.2020.07.007

Krstić Milošević D, Banjac N, Janković T, Eler K, Vinterhalter B. Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation.. in Plant Physiology and Biochemistry. 2020;154:735-744.
doi:10.1016/j.plaphy.2020.07.007 .

Krstić Milošević, Dijana, Banjac, Nevena, Janković, Teodora, Eler, Klemen, Vinterhalter, Branka, "Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation." in Plant Physiology and Biochemistry, 154 (2020):735-744,
https://doi.org/10.1016/j.plaphy.2020.07.007 . .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Savić, Jelena
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Girek, Zdenka
AU  - Adžić, Slađan
AU  - Zečević, Bogoljub
AU  - Banjac, Nevena
PY  - 2020
UR  - https://doi.org/10.1007/s11240-020-01948-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4011
AB  - Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression of NPK1 transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. The Agrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring the NPK1 and phosphinothricin N-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of both NPK1 and bar transgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level of NPK1 gene expression estimated by RT-qPCR, and the L2 line with the highest NPK1 expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of the bar gene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field. Key message The results underlined that constitutively expressing NPK1 can significantly contribute to enhanced salt stresstolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerancecruciferous vegetable lines.
PB  - Springer Science and Business Media LLC
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower
IS  - 3
VL  - 143
DO  - 10.1007/s11240-020-01948-6
SP  - 635
EP  - 651
ER  - 

@article{
author = "Pavlović, Suzana and Savić, Jelena and Milojević, Jelena and Vinterhalter, Branka and Girek, Zdenka and Adžić, Slađan and Zečević, Bogoljub and Banjac, Nevena",
year = "2020",
abstract = "Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression of NPK1 transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. The Agrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring the NPK1 and phosphinothricin N-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of both NPK1 and bar transgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level of NPK1 gene expression estimated by RT-qPCR, and the L2 line with the highest NPK1 expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of the bar gene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field. Key message The results underlined that constitutively expressing NPK1 can significantly contribute to enhanced salt stresstolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerancecruciferous vegetable lines.",
publisher = "Springer Science and Business Media LLC",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower",
number = "3",
volume = "143",
doi = "10.1007/s11240-020-01948-6",
pages = "635-651"
}

Pavlović, S., Savić, J., Milojević, J., Vinterhalter, B., Girek, Z., Adžić, S., Zečević, B.,& Banjac, N.. (2020). Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell, Tissue and Organ Culture (PCTOC)
Springer Science and Business Media LLC., 143(3), 635-651.
https://doi.org/10.1007/s11240-020-01948-6

Pavlović S, Savić J, Milojević J, Vinterhalter B, Girek Z, Adžić S, Zečević B, Banjac N. Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell, Tissue and Organ Culture (PCTOC). 2020;143(3):635-651.
doi:10.1007/s11240-020-01948-6 .

Pavlović, Suzana, Savić, Jelena, Milojević, Jelena, Vinterhalter, Branka, Girek, Zdenka, Adžić, Slađan, Zečević, Bogoljub, Banjac, Nevena, "Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower" in Plant Cell, Tissue and Organ Culture (PCTOC), 143, no. 3 (2020):635-651,
https://doi.org/10.1007/s11240-020-01948-6 . .

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Janošević, Dušica
AU  - Savić, Jelena
AU  - Todorović, Slađana
AU  - Banjac, Nevena
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3774
AB  - Gibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 µM α-naphthaleneacetic acid (NAA) and 0–10 µM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1 and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1 and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expression levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.
PB  - Springer
T2  - Plant Cell, Tissue and Organ Culture
T1  - Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach
VL  - 142
DO  - 10.1007/s11240-020-01878-3
SP  - 537
EP  - 548
ER  - 

@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Janošević, Dušica and Savić, Jelena and Todorović, Slađana and Banjac, Nevena and Milojević, Jelena",
year = "2020",
abstract = "Gibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 µM α-naphthaleneacetic acid (NAA) and 0–10 µM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1 and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1 and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expression levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.",
publisher = "Springer",
journal = "Plant Cell, Tissue and Organ Culture",
title = "Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach",
volume = "142",
doi = "10.1007/s11240-020-01878-3",
pages = "537-548"
}

Belić, M., Zdravković-Korać, S., Janošević, D., Savić, J., Todorović, S., Banjac, N.,& Milojević, J.. (2020). Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach. in Plant Cell, Tissue and Organ Culture
Springer., 142, 537-548.
https://doi.org/10.1007/s11240-020-01878-3

Belić M, Zdravković-Korać S, Janošević D, Savić J, Todorović S, Banjac N, Milojević J. Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach. in Plant Cell, Tissue and Organ Culture. 2020;142:537-548.
doi:10.1007/s11240-020-01878-3 .

Belić, Maja, Zdravković-Korać, Snežana, Janošević, Dušica, Savić, Jelena, Todorović, Slađana, Banjac, Nevena, Milojević, Jelena, "Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach" in Plant Cell, Tissue and Organ Culture, 142 (2020):537-548,
https://doi.org/10.1007/s11240-020-01878-3 . .

TY  - JOUR
AU  - Stanišić, Mariana
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Krstić Milošević, Dijana
AU  - Mišić, Danijela
AU  - Smigocki, Ann
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3791
AB  - Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequently
use the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficult
to perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root cultures
of apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation of
apple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain
15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be used
as a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidate
the possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,
kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected in
untransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry
(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production in
apple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicals
exuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantly
affected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days of
treatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsis
shoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanent
in vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.
PB  - Oxford:Oxford University Press
T2  - Tree Physiology
T1  - Hairy root culture as a valuable tool for allelopathic studies in apple
IS  - 5
VL  - 39
DO  - doi:10.1093/treephys/tpz006
SP  - 888
EP  - 905
ER  - 

@article{
author = "Stanišić, Mariana and Ćosić, Tatjana and Savić, Jelena and Krstić Milošević, Dijana and Mišić, Danijela and Smigocki, Ann and Ninković, Slavica and Banjac, Nevena",
year = "2019",
abstract = "Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequently
use the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficult
to perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root cultures
of apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation of
apple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain
15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be used
as a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidate
the possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,
kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected in
untransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry
(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production in
apple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicals
exuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantly
affected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days of
treatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsis
shoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanent
in vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.",
publisher = "Oxford:Oxford University Press",
journal = "Tree Physiology",
title = "Hairy root culture as a valuable tool for allelopathic studies in apple",
number = "5",
volume = "39",
doi = "doi:10.1093/treephys/tpz006",
pages = "888-905"
}

Stanišić, M., Ćosić, T., Savić, J., Krstić Milošević, D., Mišić, D., Smigocki, A., Ninković, S.,& Banjac, N.. (2019). Hairy root culture as a valuable tool for allelopathic studies in apple. in Tree Physiology
Oxford:Oxford University Press., 39(5), 888-905.
https://doi.org/doi:10.1093/treephys/tpz006

Stanišić M, Ćosić T, Savić J, Krstić Milošević D, Mišić D, Smigocki A, Ninković S, Banjac N. Hairy root culture as a valuable tool for allelopathic studies in apple. in Tree Physiology. 2019;39(5):888-905.
doi:doi:10.1093/treephys/tpz006 .

Stanišić, Mariana, Ćosić, Tatjana, Savić, Jelena, Krstić Milošević, Dijana, Mišić, Danijela, Smigocki, Ann, Ninković, Slavica, Banjac, Nevena, "Hairy root culture as a valuable tool for allelopathic studies in apple" in Tree Physiology, 39, no. 5 (2019):888-905,
https://doi.org/doi:10.1093/treephys/tpz006 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Ghalawenji, Nabil
AU  - Zdravković-Korać, Snežana
PY  - 2019
UR  - http://link.springer.com/10.1007/s11240-019-01595-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3310
AB  - Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)
DO  - 10.1007/s11240-019-01595-6
ER  - 

@article{
author = "Banjac, Nevena and Vinterhalter, Branka and Krstić Milošević, Dijana and Milojević, Jelena and Tubić, Ljiljana and Ghalawenji, Nabil and Zdravković-Korać, Snežana",
year = "2019",
abstract = "Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)",
doi = "10.1007/s11240-019-01595-6"
}

Banjac, N., Vinterhalter, B., Krstić Milošević, D., Milojević, J., Tubić, L., Ghalawenji, N.,& Zdravković-Korać, S.. (2019). Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). in Plant Cell, Tissue and Organ Culture (PCTOC).
https://doi.org/10.1007/s11240-019-01595-6

Banjac N, Vinterhalter B, Krstić Milošević D, Milojević J, Tubić L, Ghalawenji N, Zdravković-Korać S. Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). in Plant Cell, Tissue and Organ Culture (PCTOC). 2019;.
doi:10.1007/s11240-019-01595-6 .

Banjac, Nevena, Vinterhalter, Branka, Krstić Milošević, Dijana, Milojević, Jelena, Tubić, Ljiljana, Ghalawenji, Nabil, Zdravković-Korać, Snežana, "Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)" in Plant Cell, Tissue and Organ Culture (PCTOC) (2019),
https://doi.org/10.1007/s11240-019-01595-6 . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
AU  - Zdravković-Korać, Snežana
AU  - Banjac, Nevena
AU  - Vinterhalter, Dragan
AU  - Krstić Milošević, Dijana
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0926669018311282?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3230
AB  - Production of innovative drugs from natural products in controlled conditions plays an important role in modern pharmacology in order to tackle global health challenges. The potential of Gentiana utriculosa hairy roots, obtained by transformation with Agrobacterium rhizogenes A4M70GUS, as well as that of the shoots regenerated from them via somatic embryogenesis, for xanthone production was investigated. Gentiana utriculosa was shown to be a new source of xanthones, medicinal raw materials for different pharmaceutical applications, among which decussatin has been recently recognized as a prospective hepatoprotective and antiulcer compound. Decussatin and decussatin-1-O-primeveroside were detected in both hairy roots and transgenic shoots, while mangiferin, present in nontransformed plants, was not detected. Quantitative HPLC analysis revealed up to 4.5-fold higher decussatin production in hairy roots line 9 compared with the plants from the nature, and this clone was selected. It showed stable growth after more than two years of continuous subcultivation. Cultivation of hairy roots on solid medium was favorable for biomass production, while liquid culture was beneficial for decussatin and decussatin-1-O-primeveroside accumulation. Cytokinins kinetin or N6-benzyladenine promoted somatic embryo maturation and germination as well as multiplication of obtained plantlets, while active charcoal reduced hyperhydricity. Plants regenerated from selected hairy root line 9 comprised two TL-DNA inserts, as confirmed by Southern blot analysis. They accumulated at least 2.5-fold more decussatin then nontransformed plants. Therefore, these plants could be valuable material to create xanthone high-yielding cultivars of G. utriculosa.
T2  - Industrial Crops and Products
T1  - Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants
VL  - 130
DO  - 10.1016/J.INDCROP.2018.12.066
SP  - 216
EP  - 229
ER  - 

@article{
author = "Vinterhalter, Branka and Savić, Jelena and Zdravković-Korać, Snežana and Banjac, Nevena and Vinterhalter, Dragan and Krstić Milošević, Dijana",
year = "2019",
abstract = "Production of innovative drugs from natural products in controlled conditions plays an important role in modern pharmacology in order to tackle global health challenges. The potential of Gentiana utriculosa hairy roots, obtained by transformation with Agrobacterium rhizogenes A4M70GUS, as well as that of the shoots regenerated from them via somatic embryogenesis, for xanthone production was investigated. Gentiana utriculosa was shown to be a new source of xanthones, medicinal raw materials for different pharmaceutical applications, among which decussatin has been recently recognized as a prospective hepatoprotective and antiulcer compound. Decussatin and decussatin-1-O-primeveroside were detected in both hairy roots and transgenic shoots, while mangiferin, present in nontransformed plants, was not detected. Quantitative HPLC analysis revealed up to 4.5-fold higher decussatin production in hairy roots line 9 compared with the plants from the nature, and this clone was selected. It showed stable growth after more than two years of continuous subcultivation. Cultivation of hairy roots on solid medium was favorable for biomass production, while liquid culture was beneficial for decussatin and decussatin-1-O-primeveroside accumulation. Cytokinins kinetin or N6-benzyladenine promoted somatic embryo maturation and germination as well as multiplication of obtained plantlets, while active charcoal reduced hyperhydricity. Plants regenerated from selected hairy root line 9 comprised two TL-DNA inserts, as confirmed by Southern blot analysis. They accumulated at least 2.5-fold more decussatin then nontransformed plants. Therefore, these plants could be valuable material to create xanthone high-yielding cultivars of G. utriculosa.",
journal = "Industrial Crops and Products",
title = "Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants",
volume = "130",
doi = "10.1016/J.INDCROP.2018.12.066",
pages = "216-229"
}

Vinterhalter, B., Savić, J., Zdravković-Korać, S., Banjac, N., Vinterhalter, D.,& Krstić Milošević, D.. (2019). Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants. in Industrial Crops and Products, 130, 216-229.
https://doi.org/10.1016/J.INDCROP.2018.12.066

Vinterhalter B, Savić J, Zdravković-Korać S, Banjac N, Vinterhalter D, Krstić Milošević D. Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants. in Industrial Crops and Products. 2019;130:216-229.
doi:10.1016/J.INDCROP.2018.12.066 .

Vinterhalter, Branka, Savić, Jelena, Zdravković-Korać, Snežana, Banjac, Nevena, Vinterhalter, Dragan, Krstić Milošević, Dijana, "Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants" in Industrial Crops and Products, 130 (2019):216-229,
https://doi.org/10.1016/J.INDCROP.2018.12.066 . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Nikolić, Radomirka
AU  - Banjac, Nevena
AU  - Zdravković-Korać, Snežana
AU  - Stupar, Sofija
AU  - Cingel, Aleksandar
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161719301786?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3490
AB  - Food demands of increasing human population dictate intensification of livestock production, however, environmental stresses could jeopardize producers' efforts. Forage legumes suffer from yield losses and poor nutritional status due to salinity increase of agricultural soils. As tools aimed to reduce negative impacts of biotic or abiotic stresses, proteinase inhibitors (PIs) have been promoted for biotechnological improvements. In order to increase tolerance of Lotus corniculatus L. to salt stress, serine PI, BvSTI, was introduced into this legume using Agrobacterium rhizogenes, with final transformation efficiency of 4.57%. PCR, DNA gel-blot, RT-PCR and in-gel protein activity assays confirmed the presence and activity of BvSTI products in transformed lines. Plants from three selected transgenic lines (21, 73 and 109) showed significant alterations in overall phenotypic appearance, corresponding to differences in BvSTI accumulation. Lines 73 and 109 showed up to 7.3-fold higher number of tillers and massive, up to 5.8-fold heavier roots than in nontransformed controls (NTC). Line 21 was phenotypically similar to NTC, accumulated less BvSTI transcripts and did not exhibit an additional band of recombinant trypsin inhibitor as seen in lines 73 and 109. Exposure of the transgenic lines to NaCl revealed different levels of salt stress susceptibility. The NaCl sensitivity index, based on morphological appearance and chlorophyll concentrations showed that lines 73 and 109 were significantly less affected by salinity than NTC or line 21. High level of BvSTI altered morphology and delayed salt stress related senescence, implicating BvSTI gene as a promising tool for salinity tolerance improvement trials in L. corniculatus.
T2  - Journal of Plant Physiology
T1  - Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.
VL  - 243
DO  - 10.1016/j.jplph.2019.153055
SP  - 153055
ER  - 

@article{
author = "Savić, Jelena and Nikolić, Radomirka and Banjac, Nevena and Zdravković-Korać, Snežana and Stupar, Sofija and Cingel, Aleksandar and Ćosić, Tatjana and Raspor, Martin and Smigocki, Ann and Ninković, Slavica",
year = "2019",
abstract = "Food demands of increasing human population dictate intensification of livestock production, however, environmental stresses could jeopardize producers' efforts. Forage legumes suffer from yield losses and poor nutritional status due to salinity increase of agricultural soils. As tools aimed to reduce negative impacts of biotic or abiotic stresses, proteinase inhibitors (PIs) have been promoted for biotechnological improvements. In order to increase tolerance of Lotus corniculatus L. to salt stress, serine PI, BvSTI, was introduced into this legume using Agrobacterium rhizogenes, with final transformation efficiency of 4.57%. PCR, DNA gel-blot, RT-PCR and in-gel protein activity assays confirmed the presence and activity of BvSTI products in transformed lines. Plants from three selected transgenic lines (21, 73 and 109) showed significant alterations in overall phenotypic appearance, corresponding to differences in BvSTI accumulation. Lines 73 and 109 showed up to 7.3-fold higher number of tillers and massive, up to 5.8-fold heavier roots than in nontransformed controls (NTC). Line 21 was phenotypically similar to NTC, accumulated less BvSTI transcripts and did not exhibit an additional band of recombinant trypsin inhibitor as seen in lines 73 and 109. Exposure of the transgenic lines to NaCl revealed different levels of salt stress susceptibility. The NaCl sensitivity index, based on morphological appearance and chlorophyll concentrations showed that lines 73 and 109 were significantly less affected by salinity than NTC or line 21. High level of BvSTI altered morphology and delayed salt stress related senescence, implicating BvSTI gene as a promising tool for salinity tolerance improvement trials in L. corniculatus.",
journal = "Journal of Plant Physiology",
title = "Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.",
volume = "243",
doi = "10.1016/j.jplph.2019.153055",
pages = "153055"
}

Savić, J., Nikolić, R., Banjac, N., Zdravković-Korać, S., Stupar, S., Cingel, A., Ćosić, T., Raspor, M., Smigocki, A.,& Ninković, S.. (2019). Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.. in Journal of Plant Physiology, 243, 153055.
https://doi.org/10.1016/j.jplph.2019.153055

Savić J, Nikolić R, Banjac N, Zdravković-Korać S, Stupar S, Cingel A, Ćosić T, Raspor M, Smigocki A, Ninković S. Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.. in Journal of Plant Physiology. 2019;243:153055.
doi:10.1016/j.jplph.2019.153055 .

Savić, Jelena, Nikolić, Radomirka, Banjac, Nevena, Zdravković-Korać, Snežana, Stupar, Sofija, Cingel, Aleksandar, Ćosić, Tatjana, Raspor, Martin, Smigocki, Ann, Ninković, Slavica, "Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L." in Journal of Plant Physiology, 243 (2019):153055,
https://doi.org/10.1016/j.jplph.2019.153055 . .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Krstić Milošević, Dijana
AU  - Mišić, Danijela
AU  - Smigocki, Ann
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5009
AB  - Since the investigation of plant allelopathic interactions in the rhizosphere is difficult to perform
because of its high complexity, microbial and environmental factors interactions, the generation
of autonomous root culture could be appropriate system for the studies of allelopathic potential of apple (Malus x domestica Borkh.). Thus, we have focused our efforts on the generation
of transformed hairy-roots in apple cv. Golden Delicious using the 15834 strain of Agrobacterium
rhizogenes. The agrobacteria inoculation by dipping the basal cut off end of the stem of in vitro
grown apple shoots into bacterial suspension proved to be the best option for transformation,
providing efficiency of 39.3%. Molecular characterization of hairy roots by RT-PCR analyses confirmed
their transgenic nature. In vitro conditions for apple hairy root growth and maintenance
were optimized using different mineral solutions and auxins. The liquid half-strength MS medium
with the addition of IBA at 0.025 mg L-1 was chosen for the initial stimulation of growth. For their
prolonged cultivation and maintenance, the alternation of IBA-containing and IBA-free media is
recommendable. Fast-growing lines continuously maintained in IBA-free medium were used for
further research. Potential allelochemicals including phenolic acids (chlorogenic, caffeic, syringic,
p-coumaric and ferulic), glycosilated flavonoids (phloridzin, rutin, quercitrin, isoquercitrin, kaempferol-
3-glucoside) and flavonoid aglycons (floretin, quercetin and naringenin) have been detected
by UHPLC/DAD/(+/-)HESI-MS/MS analyses in the growth medium, in which these transformed
roots were maintained for 4 weeks, as well as in the root tissue itself. The obtained hairy-root
cultures offer a complementary system for studying and validating the allelopathic potential of
apple roots.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Transgenic hairy root in vitro culture system of apple as a tool for allelopathic studies
SP  - 55
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5009
ER  - 

@conference{
author = "Stanišić, Mariana and Ćosić, Tatjana and Savić, Jelena and Krstić Milošević, Dijana and Mišić, Danijela and Smigocki, Ann and Ninković, Slavica and Banjac, Nevena",
year = "2018",
abstract = "Since the investigation of plant allelopathic interactions in the rhizosphere is difficult to perform
because of its high complexity, microbial and environmental factors interactions, the generation
of autonomous root culture could be appropriate system for the studies of allelopathic potential of apple (Malus x domestica Borkh.). Thus, we have focused our efforts on the generation
of transformed hairy-roots in apple cv. Golden Delicious using the 15834 strain of Agrobacterium
rhizogenes. The agrobacteria inoculation by dipping the basal cut off end of the stem of in vitro
grown apple shoots into bacterial suspension proved to be the best option for transformation,
providing efficiency of 39.3%. Molecular characterization of hairy roots by RT-PCR analyses confirmed
their transgenic nature. In vitro conditions for apple hairy root growth and maintenance
were optimized using different mineral solutions and auxins. The liquid half-strength MS medium
with the addition of IBA at 0.025 mg L-1 was chosen for the initial stimulation of growth. For their
prolonged cultivation and maintenance, the alternation of IBA-containing and IBA-free media is
recommendable. Fast-growing lines continuously maintained in IBA-free medium were used for
further research. Potential allelochemicals including phenolic acids (chlorogenic, caffeic, syringic,
p-coumaric and ferulic), glycosilated flavonoids (phloridzin, rutin, quercitrin, isoquercitrin, kaempferol-
3-glucoside) and flavonoid aglycons (floretin, quercetin and naringenin) have been detected
by UHPLC/DAD/(+/-)HESI-MS/MS analyses in the growth medium, in which these transformed
roots were maintained for 4 weeks, as well as in the root tissue itself. The obtained hairy-root
cultures offer a complementary system for studying and validating the allelopathic potential of
apple roots.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Transgenic hairy root in vitro culture system of apple as a tool for allelopathic studies",
pages = "55-56",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5009"
}

Stanišić, M., Ćosić, T., Savić, J., Krstić Milošević, D., Mišić, D., Smigocki, A., Ninković, S.,& Banjac, N.. (2018). Transgenic hairy root in vitro culture system of apple as a tool for allelopathic studies. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 55-56.
https://hdl.handle.net/21.15107/rcub_ibiss_5009

Stanišić M, Ćosić T, Savić J, Krstić Milošević D, Mišić D, Smigocki A, Ninković S, Banjac N. Transgenic hairy root in vitro culture system of apple as a tool for allelopathic studies. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:55-56.
https://hdl.handle.net/21.15107/rcub_ibiss_5009 .

Stanišić, Mariana, Ćosić, Tatjana, Savić, Jelena, Krstić Milošević, Dijana, Mišić, Danijela, Smigocki, Ann, Ninković, Slavica, Banjac, Nevena, "Transgenic hairy root in vitro culture system of apple as a tool for allelopathic studies" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):55-56,
https://hdl.handle.net/21.15107/rcub_ibiss_5009 .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Smigocki, Ann
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4999
AB  - In the previous study, phenolic acids and polyphenolic flavonoids were identified as putative
allelochemicals in apple cv. Golden Delicious hairy roots growth medium. In order to examine
their allelopathic potential, the effect of hairy root exudates on the germination and seedling
growth of the test species Arabidopsis thaliana was elucidated. Arabidopsis seeds were cultivated
in Erlenmeyer flasks containing 25 mL of liquid medium in which apple hairy roots were cultivated
for 4 weeks. The effect of apple hairy root growth medium on Arabidopsis was evaluated after 5
and 10 days of seed cultivation. Apple hairy root exudates had no significant effect on Arabidopsis
germination rate, while growth characteristics of seedlings were significantly affected both after
5 and 10 days of treatment. The roots of treated seedlings were significantly shorter (35.1%), with
lower number (74% decrement) of lateral roots compared to control after10 days of treatment.
The growth and morphology of Arabidopsis shoots and leaves have been affected too. Thus, the
shoots of treated seedlings were almost twice shorter compared to control after 5 days of treatment. At the 10th day, this distinction became more pronounced, when two fully expanded leaves
were visible in treated seedlings, while control had completely developed rosettes with six leaves.
In addition, leaves of treated seedlings were smaller and pale in colour compared to control. qPCR
expression analysis of genes involved in regulation of the cell cycle showed reduction of transcript
levels in roots of treated seedlings, with the highest expression decrease detected for CDKA1 and
CYCB2;4 cyclin.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade
T1  - Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro
SP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4999
ER  - 

@conference{
author = "Stanišić, Mariana and Ćosić, Tatjana and Savić, Jelena and Smigocki, Ann and Ninković, Slavica and Banjac, Nevena",
year = "2018",
abstract = "In the previous study, phenolic acids and polyphenolic flavonoids were identified as putative
allelochemicals in apple cv. Golden Delicious hairy roots growth medium. In order to examine
their allelopathic potential, the effect of hairy root exudates on the germination and seedling
growth of the test species Arabidopsis thaliana was elucidated. Arabidopsis seeds were cultivated
in Erlenmeyer flasks containing 25 mL of liquid medium in which apple hairy roots were cultivated
for 4 weeks. The effect of apple hairy root growth medium on Arabidopsis was evaluated after 5
and 10 days of seed cultivation. Apple hairy root exudates had no significant effect on Arabidopsis
germination rate, while growth characteristics of seedlings were significantly affected both after
5 and 10 days of treatment. The roots of treated seedlings were significantly shorter (35.1%), with
lower number (74% decrement) of lateral roots compared to control after10 days of treatment.
The growth and morphology of Arabidopsis shoots and leaves have been affected too. Thus, the
shoots of treated seedlings were almost twice shorter compared to control after 5 days of treatment. At the 10th day, this distinction became more pronounced, when two fully expanded leaves
were visible in treated seedlings, while control had completely developed rosettes with six leaves.
In addition, leaves of treated seedlings were smaller and pale in colour compared to control. qPCR
expression analysis of genes involved in regulation of the cell cycle showed reduction of transcript
levels in roots of treated seedlings, with the highest expression decrease detected for CDKA1 and
CYCB2;4 cyclin.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade",
title = "Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro",
pages = "55",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4999"
}

Stanišić, M., Ćosić, T., Savić, J., Smigocki, A., Ninković, S.,& Banjac, N.. (2018). Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro. in 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade
Belgrade: Serbian Plant Physiology Society., 55.
https://hdl.handle.net/21.15107/rcub_ibiss_4999

Stanišić M, Ćosić T, Savić J, Smigocki A, Ninković S, Banjac N. Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro. in 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade. 2018;:55.
https://hdl.handle.net/21.15107/rcub_ibiss_4999 .

Stanišić, Mariana, Ćosić, Tatjana, Savić, Jelena, Smigocki, Ann, Ninković, Slavica, Banjac, Nevena, "Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro" in 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade (2018):55,
https://hdl.handle.net/21.15107/rcub_ibiss_4999 .

TY  - JOUR
AU  - Stanišić, Mariana
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Banjac, Nevena
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700037S
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3018
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2022
AB  - Since the genetic transformation of the apple is strongly genotype-dependent and generally inefficient, the evaluation of factors affecting shoot regeneration are crucial for the establishment of a successful transformation process. In this report, we evaluated the effects of the β-lactam antibiotics meropenem and timentin on in vitro regeneration via de novo shoot organogenesis from leaf explants of apple cv. Golden Delicious, as well as on the growth of the Agrobacterium tumefaciens strain EHA 105, and compared them with the commonly used β-lactam cefotaxime. Also, we report for the first time the effect of hygromycin B as a selective agent in the domesticated apple, as regards shoot regeneration and shoot multiplication efficiency. We observed that cefotaxime and timentin at concentrations higher than 100 mg L-1 were sufficient to prevent Agrobacterium growth during a two-week period, while meropenem exhibited an inhibitory effect on bacterial growth at all tested concentrations (25-150 mg L-1). Cefotaxime at a concentration of 300 mg L-1 increased the number of regenerated shoots per explant (9.39) in comparison with the control (7.67). In contrast to cefotaxime, meropenem and timentin caused a decrease in shoot regeneration efficiency, but larger and more developed shoots were obtained on meropenem (25-125 mg L-1) after the same period of cultivation. Hygromycin B at a concentration of 5 mg L-1 or higher completely inhibited shoot regeneration and induced explant tissue necrosis. Therefore, the selection procedure with a final concentration of 4 mg L-1 throughout organogenesis and 10 mg L-1 for further shoot growth and multiplication is recommended for an efficient transformation process in apple cv. Golden Delicious.
T2  - Archives of Biological Sciences
T1  - The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious
IS  - 1
VL  - 70
DO  - 10.2298/ABS170731037S
SP  - 179
EP  - 190
ER  - 

@article{
author = "Stanišić, Mariana and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Banjac, Nevena",
year = "2018",
abstract = "Since the genetic transformation of the apple is strongly genotype-dependent and generally inefficient, the evaluation of factors affecting shoot regeneration are crucial for the establishment of a successful transformation process. In this report, we evaluated the effects of the β-lactam antibiotics meropenem and timentin on in vitro regeneration via de novo shoot organogenesis from leaf explants of apple cv. Golden Delicious, as well as on the growth of the Agrobacterium tumefaciens strain EHA 105, and compared them with the commonly used β-lactam cefotaxime. Also, we report for the first time the effect of hygromycin B as a selective agent in the domesticated apple, as regards shoot regeneration and shoot multiplication efficiency. We observed that cefotaxime and timentin at concentrations higher than 100 mg L-1 were sufficient to prevent Agrobacterium growth during a two-week period, while meropenem exhibited an inhibitory effect on bacterial growth at all tested concentrations (25-150 mg L-1). Cefotaxime at a concentration of 300 mg L-1 increased the number of regenerated shoots per explant (9.39) in comparison with the control (7.67). In contrast to cefotaxime, meropenem and timentin caused a decrease in shoot regeneration efficiency, but larger and more developed shoots were obtained on meropenem (25-125 mg L-1) after the same period of cultivation. Hygromycin B at a concentration of 5 mg L-1 or higher completely inhibited shoot regeneration and induced explant tissue necrosis. Therefore, the selection procedure with a final concentration of 4 mg L-1 throughout organogenesis and 10 mg L-1 for further shoot growth and multiplication is recommended for an efficient transformation process in apple cv. Golden Delicious.",
journal = "Archives of Biological Sciences",
title = "The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious",
number = "1",
volume = "70",
doi = "10.2298/ABS170731037S",
pages = "179-190"
}

Stanišić, M., Ninković, S., Savić, J., Ćosić, T.,& Banjac, N.. (2018). The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious. in Archives of Biological Sciences, 70(1), 179-190.
https://doi.org/10.2298/ABS170731037S

Stanišić M, Ninković S, Savić J, Ćosić T, Banjac N. The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious. in Archives of Biological Sciences. 2018;70(1):179-190.
doi:10.2298/ABS170731037S .

Stanišić, Mariana, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Banjac, Nevena, "The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious" in Archives of Biological Sciences, 70, no. 1 (2018):179-190,
https://doi.org/10.2298/ABS170731037S . .