TY  - CONF
AU  - Ćirić, Jelena
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Perović, Milka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5847
AB  - Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s disease (AD) is crucial due to the lack of effective management and treatment modalities. Higher prevalence, progression rate and severity of AD in women than in men also establish sex as key variable in AD therapy development.Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to nine-fold higher prevalence in women compared to men. The molecular mechanisms by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain however elusive. We therefore examined sex-related alterations in gene expression of iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA level was evident in female WT mice, while a trend toward a decrease was detected in their APPNL-G-F KI littermates. Expression in the opposite direction was observed for TTR, with a robust genotype-dependent decrease in male mice.Results are in line with well-established role of THs in the regulation of neuronal plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Thyroid hormone metabolism in the cortex of male and female APP knock-in mice
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5847
ER  - 

@conference{
author = "Ćirić, Jelena and Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Filipović, Branko and Šošić-Jurjević, Branka  and Perović, Milka",
year = "2023",
abstract = "Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s disease (AD) is crucial due to the lack of effective management and treatment modalities. Higher prevalence, progression rate and severity of AD in women than in men also establish sex as key variable in AD therapy development.Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to nine-fold higher prevalence in women compared to men. The molecular mechanisms by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain however elusive. We therefore examined sex-related alterations in gene expression of iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA level was evident in female WT mice, while a trend toward a decrease was detected in their APPNL-G-F KI littermates. Expression in the opposite direction was observed for TTR, with a robust genotype-dependent decrease in male mice.Results are in line with well-established role of THs in the regulation of neuronal plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5847"
}

Ćirić, J., Milovanović, N., Jovanović Macura, I., Tešić, V., Filipović, B., Šošić-Jurjević, B.,& Perović, M.. (2023). Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5847

Ćirić J, Milovanović N, Jovanović Macura I, Tešić V, Filipović B, Šošić-Jurjević B, Perović M. Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5847 .

Ćirić, Jelena, Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Filipović, Branko, Šošić-Jurjević, Branka , Perović, Milka, "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice" in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5847 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Perović, Milka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5846
AB  - Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s 
disease (AD) is crucial due to the lack of effective management and treatment 
modalities. Higher prevalence, progression rate and severity of AD in women than in 
men also establish sex as key variable in AD therapy development.
Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to 
nine-fold higher prevalence in women compared to men. The molecular mechanisms 
by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain 
however elusive. We therefore examined sex-related alterations in gene expression of 
iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue 
metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, 
state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.
Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and 
TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA 
level was evident in female WT mice, while a trend toward a decrease was detected in 
their APPNL-G-F KI littermates. Expression in the opposite direction was observed for 
TTR, with a robust genotype-dependent decrease in male mice.
Results are in line with well-established role of THs in the regulation of neuronal 
plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Thyroid hormone metabolism in the cortex of male and female APP knock-in mice
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5846
ER  - 

@conference{
author = "Ćirić, Jelena and Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Filipović, Branko and Šošić-Jurjević, Branka  and Perović, Milka",
year = "2023",
abstract = "Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s 
disease (AD) is crucial due to the lack of effective management and treatment 
modalities. Higher prevalence, progression rate and severity of AD in women than in 
men also establish sex as key variable in AD therapy development.
Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to 
nine-fold higher prevalence in women compared to men. The molecular mechanisms 
by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain 
however elusive. We therefore examined sex-related alterations in gene expression of 
iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue 
metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, 
state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.
Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and 
TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA 
level was evident in female WT mice, while a trend toward a decrease was detected in 
their APPNL-G-F KI littermates. Expression in the opposite direction was observed for 
TTR, with a robust genotype-dependent decrease in male mice.
Results are in line with well-established role of THs in the regulation of neuronal 
plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5846"
}

Ćirić, J., Milovanović, N., Jovanović Macura, I., Tešić, V., Filipović, B., Šošić-Jurjević, B.,& Perović, M.. (2023). Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5846

Ćirić J, Milovanović N, Jovanović Macura I, Tešić V, Filipović B, Šošić-Jurjević B, Perović M. Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5846 .

Ćirić, Jelena, Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Filipović, Branko, Šošić-Jurjević, Branka , Perović, Milka, "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5846 .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pavković, Željko
AU  - Perović, Milka
AU  - Pešić, Vesna
AU  - Ćirić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5842
AB  - Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main 
cause of dementia in the elderly worldwide. It is also well-established that the 
prevalence and severity of AD is greater in women than in men, suggesting that sex is 
a crucial variable in disease heterogeneity.
Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like 
amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were 
assessed using novel object recognition (NOR) and relocation test (NOL), 
respectively, while for depressive-like behavior tail-suspension test (TST) was used. 
Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were 
tested at the age of 9 months.
Memory impairments were evident in both WT and APPNL-G-F females in comparison 
to their male littermates. In NOL as a spatial variation of NOR, the discrimination 
index was decreased, but not in NOR as such. Furthermore, the decrease in total active 
immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. 
male mice suggesting more prominent depressive-like behavior as well. Examined 
parameters had similar pattern in WT and APPNL-G-F mice of both sexes. 
The results suggest prominent sex-biased differences in behavior of males and females 
in this particular model and support its validity for further studies revealing the impact 
of sex to behavioral deficits.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5842
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pavković, Željko and Perović, Milka and Pešić, Vesna and Ćirić, Jelena",
year = "2023",
abstract = "Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main 
cause of dementia in the elderly worldwide. It is also well-established that the 
prevalence and severity of AD is greater in women than in men, suggesting that sex is 
a crucial variable in disease heterogeneity.
Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like 
amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were 
assessed using novel object recognition (NOR) and relocation test (NOL), 
respectively, while for depressive-like behavior tail-suspension test (TST) was used. 
Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were 
tested at the age of 9 months.
Memory impairments were evident in both WT and APPNL-G-F females in comparison 
to their male littermates. In NOL as a spatial variation of NOR, the discrimination 
index was decreased, but not in NOR as such. Furthermore, the decrease in total active 
immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. 
male mice suggesting more prominent depressive-like behavior as well. Examined 
parameters had similar pattern in WT and APPNL-G-F mice of both sexes. 
The results suggest prominent sex-biased differences in behavior of males and females 
in this particular model and support its validity for further studies revealing the impact 
of sex to behavioral deficits.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5842"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pavković, Ž., Perović, M., Pešić, V.,& Ćirić, J.. (2023). The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 110.
https://hdl.handle.net/21.15107/rcub_ibiss_5842

Milovanović N, Jovanović Macura I, Tešić V, Pavković Ž, Perović M, Pešić V, Ćirić J. The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:110.
https://hdl.handle.net/21.15107/rcub_ibiss_5842 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pavković, Željko, Perović, Milka, Pešić, Vesna, Ćirić, Jelena, "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):110,
https://hdl.handle.net/21.15107/rcub_ibiss_5842 .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pavković, Željko
AU  - Perović, Milka
AU  - Pešić, Vesna
AU  - Ćirić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5843
AB  - Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main cause of dementia in the elderly worldwide. It is also well-established that the prevalence and severity of AD is greater in women than in men, suggesting that sex is a crucial variable in disease heterogeneity.Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were assessed using novel object recognition (NOR) and relocation test (NOL), respectively, while for depressive-like behavior tail-suspension test (TST) was used. Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were tested at the age of 9 months.Memory impairments were evident in both WT and APPNL-G-F females in comparison to their male littermates. In NOL as a spatial variation of NOR, the discrimination index was decreased, but not in NOR as such. Furthermore, the decrease in total active immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. male mice suggesting more prominent depressive-like behavior as well. Examined parameters had similar pattern in WT and APPNL-G-F mice of both sexes. The results suggest prominent sex-biased differences in behavior of males and females in this particular model and support its validity for further studies revealing the impact of sex to behavioral deficits.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5843
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pavković, Željko and Perović, Milka and Pešić, Vesna and Ćirić, Jelena",
year = "2023",
abstract = "Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main cause of dementia in the elderly worldwide. It is also well-established that the prevalence and severity of AD is greater in women than in men, suggesting that sex is a crucial variable in disease heterogeneity.Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were assessed using novel object recognition (NOR) and relocation test (NOL), respectively, while for depressive-like behavior tail-suspension test (TST) was used. Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were tested at the age of 9 months.Memory impairments were evident in both WT and APPNL-G-F females in comparison to their male littermates. In NOL as a spatial variation of NOR, the discrimination index was decreased, but not in NOR as such. Furthermore, the decrease in total active immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. male mice suggesting more prominent depressive-like behavior as well. Examined parameters had similar pattern in WT and APPNL-G-F mice of both sexes. The results suggest prominent sex-biased differences in behavior of males and females in this particular model and support its validity for further studies revealing the impact of sex to behavioral deficits.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5843"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pavković, Ž., Perović, M., Pešić, V.,& Ćirić, J.. (2023). The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5843

Milovanović N, Jovanović Macura I, Tešić V, Pavković Ž, Perović M, Pešić V, Ćirić J. The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5843 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pavković, Željko, Perović, Milka, Pešić, Vesna, Ćirić, Jelena, "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease" in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5843 .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Ivković, Sanja
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9599456
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5172
AB  - Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.
PB  - Basel: MDPI
T2  - Brain Sciences
T1  - Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging
IS  - 10
VL  - 12
DO  - 10.3390/brainsci12101297
SP  - 1297
ER  - 

@article{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Ivković, Sanja and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.",
publisher = "Basel: MDPI",
journal = "Brain Sciences",
title = "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging",
number = "10",
volume = "12",
doi = "10.3390/brainsci12101297",
pages = "1297"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Ivković, S., Kanazir, S.,& Perović, M.. (2022). Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences
Basel: MDPI., 12(10), 1297.
https://doi.org/10.3390/brainsci12101297

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Ivković S, Kanazir S, Perović M. Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences. 2022;12(10):1297.
doi:10.3390/brainsci12101297 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Ivković, Sanja, Kanazir, Selma, Perović, Milka, "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging" in Brain Sciences, 12, no. 10 (2022):1297,
https://doi.org/10.3390/brainsci12101297 . .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pešić, Vesna
AU  - Hofman, Katarina
AU  - Perović, Milka
AU  - Ćirić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5616
AB  - Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење које 
карактеришу бројни когнитивни поремећаји и промене у  понашању. Главни је 
узрок деменције код старих особа, а услед пораста просечне старости популације, 
очекује се и да ће број оболелих бити дуплиран у следеће две деценије. У циљу 
разумевања патолошких механизама у основи овог обољења, као и испитивања 
потенцијалних терапијских приступа, развијен је велики број трансгених мишјих 
модела АБ. APPNL-G-F мишеви су „knock-in“ модел АБ новије генерације које 
карактерише присуство хуманог амилоидног прекурсорског протеина (АРР) са три 
мутације које се јављају у  фамилијарном облику болести. Модел карактерише 
убрзан ток патолошких промена, односно депоновање амилоида и појава глиозе 
већ код животиња старих 2 месеца. Да би се окарактерисале ране промене у 
понашању, код мужјака APPNL-G-F мишева  старих 7 месеци и њихових контрола 
дивљег типа из истих окота, испитиване су локомоторна активност и краткотрајна 
меморија. Локомоторна активност је испитивана Тестом отвореног поља, док је 
краткотрајна меморија испитивана Тестом препознавања новог објекта. Додатно, 
један од објеката у тесту отвореног поља је накнадно померан, чинећи тест 
погодним за испитивање краткотрајне визуелне и просторне меморије. Резултати 
су указали на значајне промене у понашању које могу послужити као рани маркери 
когнитивних дефицита у овом моделу.
AB  - Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje koje karakterišu brojni kognitivni poremećaji i promene u ponašanju. Glavni je uzrok demencije kod starih osoba, a usled porasta prosečne starosti populacije, očekuje se i da će broj obolelih biti dupliran u sledeće dve decenije. U cilju razumevanja patoloških mehanizama u osnovi ovog oboljenja, kao i ispitivanja potencijalnih terapijskih pristupa, razvijen je veliki broj transgenih mišjih modela AB. APPNL-G-F miševi su „knock-in“ model AB novije generacije koje karakteriše prisustvo humanog amiloidnog prekursorskog proteina (ARR) sa tri mutacije koje se javljaju u familijarnom obliku bolesti. Model karakteriše ubrzan tok patoloških promena, odnosno deponovanje amiloida i pojava glioze već kod životinja starih 2 meseca. Da bi se okarakterisale rane promene u ponašanju, kod mužjaka APPNL-G-F miševa starih 7 meseci i njihovih kontrola divljeg tipa iz istih okota, ispitivane su lokomotorna aktivnost i kratkotrajna memorija. Lokomotorna aktivnost je ispitivana Testom otvorenog polja, dok je kratkotrajna memorija ispitivana Testom prepoznavanja novog objekta. Dodatno, jedan od objekata u testu otvorenog polja je naknadno pomeran, čineći test pogodnim za ispitivanje kratkotrajne vizuelne i prostorne memorije. Rezultati su ukazali na značajne promene u ponašanju koje mogu poslužiti kao rani markeri kognitivnih deficita u ovom modelu.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti
T1  - Карактеризација локомоторне активности и краткотрајне меморије APPNL-G-F мишева као анималног модела Алцхајмерове болести
SP  - 382
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5616
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pešić, Vesna and Hofman, Katarina and Perović, Milka and Ćirić, Jelena",
year = "2022",
abstract = "Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење које 
карактеришу бројни когнитивни поремећаји и промене у  понашању. Главни је 
узрок деменције код старих особа, а услед пораста просечне старости популације, 
очекује се и да ће број оболелих бити дуплиран у следеће две деценије. У циљу 
разумевања патолошких механизама у основи овог обољења, као и испитивања 
потенцијалних терапијских приступа, развијен је велики број трансгених мишјих 
модела АБ. APPNL-G-F мишеви су „knock-in“ модел АБ новије генерације које 
карактерише присуство хуманог амилоидног прекурсорског протеина (АРР) са три 
мутације које се јављају у  фамилијарном облику болести. Модел карактерише 
убрзан ток патолошких промена, односно депоновање амилоида и појава глиозе 
већ код животиња старих 2 месеца. Да би се окарактерисале ране промене у 
понашању, код мужјака APPNL-G-F мишева  старих 7 месеци и њихових контрола 
дивљег типа из истих окота, испитиване су локомоторна активност и краткотрајна 
меморија. Локомоторна активност је испитивана Тестом отвореног поља, док је 
краткотрајна меморија испитивана Тестом препознавања новог објекта. Додатно, 
један од објеката у тесту отвореног поља је накнадно померан, чинећи тест 
погодним за испитивање краткотрајне визуелне и просторне меморије. Резултати 
су указали на значајне промене у понашању које могу послужити као рани маркери 
когнитивних дефицита у овом моделу., Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje koje karakterišu brojni kognitivni poremećaji i promene u ponašanju. Glavni je uzrok demencije kod starih osoba, a usled porasta prosečne starosti populacije, očekuje se i da će broj obolelih biti dupliran u sledeće dve decenije. U cilju razumevanja patoloških mehanizama u osnovi ovog oboljenja, kao i ispitivanja potencijalnih terapijskih pristupa, razvijen je veliki broj transgenih mišjih modela AB. APPNL-G-F miševi su „knock-in“ model AB novije generacije koje karakteriše prisustvo humanog amiloidnog prekursorskog proteina (ARR) sa tri mutacije koje se javljaju u familijarnom obliku bolesti. Model karakteriše ubrzan tok patoloških promena, odnosno deponovanje amiloida i pojava glioze već kod životinja starih 2 meseca. Da bi se okarakterisale rane promene u ponašanju, kod mužjaka APPNL-G-F miševa starih 7 meseci i njihovih kontrola divljeg tipa iz istih okota, ispitivane su lokomotorna aktivnost i kratkotrajna memorija. Lokomotorna aktivnost je ispitivana Testom otvorenog polja, dok je kratkotrajna memorija ispitivana Testom prepoznavanja novog objekta. Dodatno, jedan od objekata u testu otvorenog polja je naknadno pomeran, čineći test pogodnim za ispitivanje kratkotrajne vizuelne i prostorne memorije. Rezultati su ukazali na značajne promene u ponašanju koje mogu poslužiti kao rani markeri kognitivnih deficita u ovom modelu.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti, Карактеризација локомоторне активности и краткотрајне меморије APPNL-G-F мишева као анималног модела Алцхајмерове болести",
pages = "382",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5616"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pešić, V., Hofman, K., Perović, M.,& Ćirić, J.. (2022). Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 382.
https://hdl.handle.net/21.15107/rcub_ibiss_5616

Milovanović N, Jovanović Macura I, Tešić V, Pešić V, Hofman K, Perović M, Ćirić J. Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:382.
https://hdl.handle.net/21.15107/rcub_ibiss_5616 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pešić, Vesna, Hofman, Katarina, Perović, Milka, Ćirić, Jelena, "Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):382,
https://hdl.handle.net/21.15107/rcub_ibiss_5616 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Hofman, Katarina
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5613
AB  - Дуготрајна рестрикција уноса хране повољно делује на организам у целини, а 
показанa су и бројна неуропротективна дејства овог режима исхране на обољења 
повезана са старењем, попут Алцхајмерове болести. Циљ ове студије је био да се 
испита превентивно дејство рестрикције уноса хране и утицај на парвалбуминске 
неуроне (PV) коре великог мозга и BDNF/ТrkB сигнални пут у трансгеном моделу 
Алцхајмерове болести. Женке 5XFAD мишева и њихове не-трансгене контроле су 
биле излагане ad libidum (AL) или EOD (од енгл. Every-Other-Day feeding) режиму 
исхране почевши од другог месеца старости. Број PV неурона је одређиван 
имунохистохемијском методом у  retrosplenial dysgranular cortex  (RSD), 
retrosplenial granular cortex  (RSG),  parietal cortex  (PtA) и  somatosensory  cortex  (S) 
код животиња старих шест месеци. Код TgAL мишева је утврђено значајно 
смањење броја PV неурона у RSGc, PtA и S, док промене у броју у RSD нису 
уочене. Четири месеца ЕОD режима исхране је смањило пад у броју PV неурона у 
сва три испитивана региона. Анализа BDNF/ТrkB сигналног пута имуноблот 
поступком је такође указала на смањење BDNF-а код TgAL мишева, али и на 
додатно смањење овог протеина код TgЕОD мишева. Значајне разлике у pro-
BDNF-у (прекурсор  BDNF-а)  нису уочене. Резултати ове студије указују да 
рестрикција хране може спречити губитак PV неурона у трансгеном моделу 
Алцхајмерове болести, што доприноси и бољем разумевању неуронске  основе 
когнитивних поремећаја у овом обољењу и од значаја је за даљи развој потребних 
додатних терапијских приступа.
AB  - Dugotrajna restrikcija unosa hrane povoljno deluje na organizam u celini, a pokazana su i brojna neuroprotektivna dejstva ovog režima ishrane na oboljenja povezana sa starenjem, poput Alchajmerove bolesti. Cilj ove studije je bio da se ispita preventivno dejstvo restrikcije unosa hrane i uticaj na parvalbuminske neurone (PV) kore velikog mozga i BDNF/TrkB signalni put u transgenom modelu Alchajmerove bolesti. Ženke 5XFAD miševa i njihove ne-transgene kontrole su bile izlagane ad libidum (AL) ili EOD (od engl. Every-Other-Day feeding) režimu ishrane počevši od drugog meseca starosti. Broj PV neurona je određivan imunohistohemijskom metodom u retrosplenial dysgranular cortex (RSD), retrosplenial granular cortex (RSG), parietal cortex (PtA) i somatosensory cortex (S) kod životinja starih šest meseci. Kod TgAL miševa je utvrđeno značajno smanjenje broja PV neurona u RSGc, PtA i S, dok promene u broju u RSD nisu uočene. Četiri meseca EOD režima ishrane je smanjilo pad u broju PV neurona u sva tri ispitivana regiona. Analiza BDNF/TrkB signalnog puta imunoblot postupkom je takođe ukazala na smanjenje BDNF-a kod TgAL miševa, ali i na dodatno smanjenje ovog proteina kod TgEOD miševa. Značajne razlike u pro- BDNF-u (prekursor BDNF-a) nisu uočene. Rezultati ove studije ukazuju da restrikcija hrane može sprečiti gubitak PV neurona u transgenom modelu Alchajmerove bolesti, što doprinosi i boljem razumevanju neuronske osnove kognitivnih poremećaja u ovom oboljenju i od značaja je za dalji razvoj potrebnih dodatnih terapijskih pristupa.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti
T1  - Дејство рестрикције хране на парвалбуминске неуроне коре великог мозга у трансгеном моделу Алцхајмерове болести
SP  - 343
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5613
ER  - 

@conference{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Hofman, Katarina and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Дуготрајна рестрикција уноса хране повољно делује на организам у целини, а 
показанa су и бројна неуропротективна дејства овог режима исхране на обољења 
повезана са старењем, попут Алцхајмерове болести. Циљ ове студије је био да се 
испита превентивно дејство рестрикције уноса хране и утицај на парвалбуминске 
неуроне (PV) коре великог мозга и BDNF/ТrkB сигнални пут у трансгеном моделу 
Алцхајмерове болести. Женке 5XFAD мишева и њихове не-трансгене контроле су 
биле излагане ad libidum (AL) или EOD (од енгл. Every-Other-Day feeding) режиму 
исхране почевши од другог месеца старости. Број PV неурона је одређиван 
имунохистохемијском методом у  retrosplenial dysgranular cortex  (RSD), 
retrosplenial granular cortex  (RSG),  parietal cortex  (PtA) и  somatosensory  cortex  (S) 
код животиња старих шест месеци. Код TgAL мишева је утврђено значајно 
смањење броја PV неурона у RSGc, PtA и S, док промене у броју у RSD нису 
уочене. Четири месеца ЕОD режима исхране је смањило пад у броју PV неурона у 
сва три испитивана региона. Анализа BDNF/ТrkB сигналног пута имуноблот 
поступком је такође указала на смањење BDNF-а код TgAL мишева, али и на 
додатно смањење овог протеина код TgЕОD мишева. Значајне разлике у pro-
BDNF-у (прекурсор  BDNF-а)  нису уочене. Резултати ове студије указују да 
рестрикција хране може спречити губитак PV неурона у трансгеном моделу 
Алцхајмерове болести, што доприноси и бољем разумевању неуронске  основе 
когнитивних поремећаја у овом обољењу и од значаја је за даљи развој потребних 
додатних терапијских приступа., Dugotrajna restrikcija unosa hrane povoljno deluje na organizam u celini, a pokazana su i brojna neuroprotektivna dejstva ovog režima ishrane na oboljenja povezana sa starenjem, poput Alchajmerove bolesti. Cilj ove studije je bio da se ispita preventivno dejstvo restrikcije unosa hrane i uticaj na parvalbuminske neurone (PV) kore velikog mozga i BDNF/TrkB signalni put u transgenom modelu Alchajmerove bolesti. Ženke 5XFAD miševa i njihove ne-transgene kontrole su bile izlagane ad libidum (AL) ili EOD (od engl. Every-Other-Day feeding) režimu ishrane počevši od drugog meseca starosti. Broj PV neurona je određivan imunohistohemijskom metodom u retrosplenial dysgranular cortex (RSD), retrosplenial granular cortex (RSG), parietal cortex (PtA) i somatosensory cortex (S) kod životinja starih šest meseci. Kod TgAL miševa je utvrđeno značajno smanjenje broja PV neurona u RSGc, PtA i S, dok promene u broju u RSD nisu uočene. Četiri meseca EOD režima ishrane je smanjilo pad u broju PV neurona u sva tri ispitivana regiona. Analiza BDNF/TrkB signalnog puta imunoblot postupkom je takođe ukazala na smanjenje BDNF-a kod TgAL miševa, ali i na dodatno smanjenje ovog proteina kod TgEOD miševa. Značajne razlike u pro- BDNF-u (prekursor BDNF-a) nisu uočene. Rezultati ove studije ukazuju da restrikcija hrane može sprečiti gubitak PV neurona u transgenom modelu Alchajmerove bolesti, što doprinosi i boljem razumevanju neuronske osnove kognitivnih poremećaja u ovom oboljenju i od značaja je za dalji razvoj potrebnih dodatnih terapijskih pristupa.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti, Дејство рестрикције хране на парвалбуминске неуроне коре великог мозга у трансгеном моделу Алцхајмерове болести",
pages = "343",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5613"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Hofman, K., Kanazir, S.,& Perović, M.. (2022). Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 343.
https://hdl.handle.net/21.15107/rcub_ibiss_5613

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Hofman K, Kanazir S, Perović M. Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:343.
https://hdl.handle.net/21.15107/rcub_ibiss_5613 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Hofman, Katarina, Kanazir, Selma, Perović, Milka, "Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):343,
https://hdl.handle.net/21.15107/rcub_ibiss_5613 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5187
AB  - Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice
DO  - 10.1002/2211-5463.13440
SP  - 132
ER  - 

@conference{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice",
doi = "10.1002/2211-5463.13440",
pages = "132"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Kanazir, S.,& Perović, M.. (2022). Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 132.
https://doi.org/10.1002/2211-5463.13440

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Kanazir S, Perović M. Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:132.
doi:10.1002/2211-5463.13440 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Kanazir, Selma, Perović, Milka, "Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):132,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - JOUR
AU  - Tešić, Vesna
AU  - Ćirić, Jelena
AU  - Jovanović Macura, Irena
AU  - Zogović, Nevena
AU  - Milanović, Desanka
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2021
UR  - https://www.mdpi.com/2072-6643/13/12/4526
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8703853
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4760
AB  - Numerous beneficial effects of food restriction on aging and age-related pathologies are well documented. It is also well-established that both short- and long-term food restriction regimens induce elevated circulating levels of glucocorticoids, stress-induced hormones produced by adrenal glands that can also exert deleterious effects on the brain. In the present study, we examined the effect of long-term food restriction on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the cortex during aging, in 18- and 24-month-old rats. Corticosterone level was increased in the cortex of aged ad libitum-fed rats. Food restriction induced its further increase, accompanied with an increase in the level of 11β-hydroxysteroid dehydrogenase type 1. However, alterations in the level of GR phosphorylated at Ser232 were not detected in animals on food restriction, in line with unaltered CDK5 level, the decrease of Hsp90, and an increase in a negative regulator of GR function, FKBP51. Moreover, our data revealed that reduced food intake prevented age-related increase in the levels of NFκB, gfap, and bax, confirming its anti-inflammatory and anti-apoptotic effects. Along with an increase in the levels of c-fos, our study provides additional evidences that food restriction affects cortical responsiveness to glucocorticoids during aging.
PB  - Basel: MDPI
T2  - Nutrients
T1  - Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.
IS  - 12
VL  - 13
DO  - 10.3390/nu13124526
SP  - 4526
ER  - 

@article{
author = "Tešić, Vesna and Ćirić, Jelena and Jovanović Macura, Irena and Zogović, Nevena and Milanović, Desanka and Kanazir, Selma and Perović, Milka",
year = "2021",
abstract = "Numerous beneficial effects of food restriction on aging and age-related pathologies are well documented. It is also well-established that both short- and long-term food restriction regimens induce elevated circulating levels of glucocorticoids, stress-induced hormones produced by adrenal glands that can also exert deleterious effects on the brain. In the present study, we examined the effect of long-term food restriction on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the cortex during aging, in 18- and 24-month-old rats. Corticosterone level was increased in the cortex of aged ad libitum-fed rats. Food restriction induced its further increase, accompanied with an increase in the level of 11β-hydroxysteroid dehydrogenase type 1. However, alterations in the level of GR phosphorylated at Ser232 were not detected in animals on food restriction, in line with unaltered CDK5 level, the decrease of Hsp90, and an increase in a negative regulator of GR function, FKBP51. Moreover, our data revealed that reduced food intake prevented age-related increase in the levels of NFκB, gfap, and bax, confirming its anti-inflammatory and anti-apoptotic effects. Along with an increase in the levels of c-fos, our study provides additional evidences that food restriction affects cortical responsiveness to glucocorticoids during aging.",
publisher = "Basel: MDPI",
journal = "Nutrients",
title = "Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.",
number = "12",
volume = "13",
doi = "10.3390/nu13124526",
pages = "4526"
}

Tešić, V., Ćirić, J., Jovanović Macura, I., Zogović, N., Milanović, D., Kanazir, S.,& Perović, M.. (2021). Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.. in Nutrients
Basel: MDPI., 13(12), 4526.
https://doi.org/10.3390/nu13124526

Tešić V, Ćirić J, Jovanović Macura I, Zogović N, Milanović D, Kanazir S, Perović M. Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.. in Nutrients. 2021;13(12):4526.
doi:10.3390/nu13124526 .

Tešić, Vesna, Ćirić, Jelena, Jovanović Macura, Irena, Zogović, Nevena, Milanović, Desanka, Kanazir, Selma, Perović, Milka, "Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction." in Nutrients, 13, no. 12 (2021):4526,
https://doi.org/10.3390/nu13124526 . .

TY  - JOUR
AU  - Lazić, Divna
AU  - Tešić, Vesna
AU  - Jovanović, Mirna
AU  - Brkić, Marjana
AU  - Milanović, Desanka
AU  - Zloković, Berislav V.
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/31931140
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3596
AB  - Food restriction has been widely associated with beneficial effects on brain aging and age-related neurodegenerative diseases such as Alzheimer's disease. However, previous studies on the effects of food restriction on aging- or pathology-related cognitive decline are controversial, emphasizing the importance of the type, onset and duration of food restriction. In the present study, we assessed the effects of preventive every-other-day (EOD) feeding regimen on neurodegenerative phenotype in 5XFAD transgenic mice, a commonly used mouse model of Alzheimer's disease. EOD feeding regimen was introduced to transgenic female mice at the age of 2 months and the effects on amyloid-β (Aβ) accumulation, gliosis, synaptic plasticity, and blood-brain barrier breakdown were analyzed in cortical tissue of 6-month-old animals. Surprisingly, significant increase of inflammation in the cortex of 5XFAD fed EOD mice was observed, reflected by the expression of microglial and astrocytic markers. This increase in reactivity and/or proliferation of glial cells was accompanied by an increase in proinflammatory cytokine TNF-α, p38 MAPK and EAAT2, and a decrease in GAD67. NMDA receptor subunit 2B, related to glutamate excitotoxicity, was increased in the cortex of 5XFAD-EOD mice indicating additional alterations in glutamatergic signaling. Furthermore, 4 months of EOD feeding regimen had led to synaptic plasticity proteins reduction and neuronal injury in 5XFAD mice. However, EOD feeding regimen did not affect Aβ load and blood-brain barrier permeability in the cortex of 5XFAD mice. Our results demonstrate that EOD feeding regimen exacerbates Alzheimer's disease-like neurodegenerative and neuroinflammatory changes irrespective of Aβ pathology in 5XFAD mice, suggesting that caution should be paid when using food restrictions in the prodromal phase of this neurodegenerative disease.
T2  - Neurobiology of Disease
T1  - Every-other-day feeding exacerbates inflammation and neuronal deficits in 5XFAD mouse model of Alzheimer's disease.
VL  - 136
DO  - 10.1016/j.nbd.2020.104745
SP  - 104745
ER  - 

@article{
author = "Lazić, Divna and Tešić, Vesna and Jovanović, Mirna and Brkić, Marjana and Milanović, Desanka and Zloković, Berislav V. and Kanazir, Selma and Perović, Milka",
year = "2020",
abstract = "Food restriction has been widely associated with beneficial effects on brain aging and age-related neurodegenerative diseases such as Alzheimer's disease. However, previous studies on the effects of food restriction on aging- or pathology-related cognitive decline are controversial, emphasizing the importance of the type, onset and duration of food restriction. In the present study, we assessed the effects of preventive every-other-day (EOD) feeding regimen on neurodegenerative phenotype in 5XFAD transgenic mice, a commonly used mouse model of Alzheimer's disease. EOD feeding regimen was introduced to transgenic female mice at the age of 2 months and the effects on amyloid-β (Aβ) accumulation, gliosis, synaptic plasticity, and blood-brain barrier breakdown were analyzed in cortical tissue of 6-month-old animals. Surprisingly, significant increase of inflammation in the cortex of 5XFAD fed EOD mice was observed, reflected by the expression of microglial and astrocytic markers. This increase in reactivity and/or proliferation of glial cells was accompanied by an increase in proinflammatory cytokine TNF-α, p38 MAPK and EAAT2, and a decrease in GAD67. NMDA receptor subunit 2B, related to glutamate excitotoxicity, was increased in the cortex of 5XFAD-EOD mice indicating additional alterations in glutamatergic signaling. Furthermore, 4 months of EOD feeding regimen had led to synaptic plasticity proteins reduction and neuronal injury in 5XFAD mice. However, EOD feeding regimen did not affect Aβ load and blood-brain barrier permeability in the cortex of 5XFAD mice. Our results demonstrate that EOD feeding regimen exacerbates Alzheimer's disease-like neurodegenerative and neuroinflammatory changes irrespective of Aβ pathology in 5XFAD mice, suggesting that caution should be paid when using food restrictions in the prodromal phase of this neurodegenerative disease.",
journal = "Neurobiology of Disease",
title = "Every-other-day feeding exacerbates inflammation and neuronal deficits in 5XFAD mouse model of Alzheimer's disease.",
volume = "136",
doi = "10.1016/j.nbd.2020.104745",
pages = "104745"
}

Lazić, D., Tešić, V., Jovanović, M., Brkić, M., Milanović, D., Zloković, B. V., Kanazir, S.,& Perović, M.. (2020). Every-other-day feeding exacerbates inflammation and neuronal deficits in 5XFAD mouse model of Alzheimer's disease.. in Neurobiology of Disease, 136, 104745.
https://doi.org/10.1016/j.nbd.2020.104745

Lazić D, Tešić V, Jovanović M, Brkić M, Milanović D, Zloković BV, Kanazir S, Perović M. Every-other-day feeding exacerbates inflammation and neuronal deficits in 5XFAD mouse model of Alzheimer's disease.. in Neurobiology of Disease. 2020;136:104745.
doi:10.1016/j.nbd.2020.104745 .

Lazić, Divna, Tešić, Vesna, Jovanović, Mirna, Brkić, Marjana, Milanović, Desanka, Zloković, Berislav V., Kanazir, Selma, Perović, Milka, "Every-other-day feeding exacerbates inflammation and neuronal deficits in 5XFAD mouse model of Alzheimer's disease." in Neurobiology of Disease, 136 (2020):104745,
https://doi.org/10.1016/j.nbd.2020.104745 . .

TY  - CONF
AU  - Srbovan, Maja
AU  - Prpa, Ksenija
AU  - Tešić, Vesna
AU  - Milanović, Desanka
AU  - Perović, Milka
AU  - Kanazir, Selma
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5908
AB  - Aim: Food restriction has been widely associated with beneficial effects on brain aging and age-related neurodegenerative diseases such as Alzheimer’s disease (AD). In the present study, the effects of every-other-day (EOD) feeding regimen were studied in the hippocampus of 5XFAD mice, a well characterized animal model of AD. Parvalbumin (PV) inhibitory interneurons that are crucial for maintaining proper excitatory/inhibitory balance were examined.
Methods: Female 5xFAD mice (Tg) and their non-transgenic littermates (non-Tg) were exposed to ad libitum (AL) or intermittent, EOD feeding regimen, beginning at 2 months of age. Neurons expressing PV were detected by immunohistochemistry, in the dorsal hippocampus of 6-month-old animals. The number of parvalbumin-expressing neurons was determined independently in CA1, CA3, and DG hippocampal subregions.
Results: Immunohistochemical analysis revealed a substantial increase in the number of parvalbumin inhibitory neurons in the dorsal hippocampus of Tg-AL mice in comparison to non-Tg animals. In Tg-EOD mice, however, alterations in the number of PV-expressing neurons were subregion-specific comparing to Tg-AL mice of the same age.
Conlusions: The results of our study clearly indicate that PV-expressing interneurons are of importance in further understanding of neural basis of AD-like-associated cognitive impairments and EOD-induced effects in 5xFAD mouse model of AD.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice
IS  - 299
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5908
ER  - 

@conference{
author = "Srbovan, Maja and Prpa, Ksenija and Tešić, Vesna and Milanović, Desanka and Perović, Milka and Kanazir, Selma",
year = "2019",
abstract = "Aim: Food restriction has been widely associated with beneficial effects on brain aging and age-related neurodegenerative diseases such as Alzheimer’s disease (AD). In the present study, the effects of every-other-day (EOD) feeding regimen were studied in the hippocampus of 5XFAD mice, a well characterized animal model of AD. Parvalbumin (PV) inhibitory interneurons that are crucial for maintaining proper excitatory/inhibitory balance were examined.
Methods: Female 5xFAD mice (Tg) and their non-transgenic littermates (non-Tg) were exposed to ad libitum (AL) or intermittent, EOD feeding regimen, beginning at 2 months of age. Neurons expressing PV were detected by immunohistochemistry, in the dorsal hippocampus of 6-month-old animals. The number of parvalbumin-expressing neurons was determined independently in CA1, CA3, and DG hippocampal subregions.
Results: Immunohistochemical analysis revealed a substantial increase in the number of parvalbumin inhibitory neurons in the dorsal hippocampus of Tg-AL mice in comparison to non-Tg animals. In Tg-EOD mice, however, alterations in the number of PV-expressing neurons were subregion-specific comparing to Tg-AL mice of the same age.
Conlusions: The results of our study clearly indicate that PV-expressing interneurons are of importance in further understanding of neural basis of AD-like-associated cognitive impairments and EOD-induced effects in 5xFAD mouse model of AD.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice",
number = "299",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5908"
}

Srbovan, M., Prpa, K., Tešić, V., Milanović, D., Perović, M.,& Kanazir, S.. (2019). The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society.(299).
https://hdl.handle.net/21.15107/rcub_ibiss_5908

Srbovan M, Prpa K, Tešić V, Milanović D, Perović M, Kanazir S. The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;(299).
https://hdl.handle.net/21.15107/rcub_ibiss_5908 .

Srbovan, Maja, Prpa, Ksenija, Tešić, Vesna, Milanović, Desanka, Perović, Milka, Kanazir, Selma, "The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia, no. 299 (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_5908 .

TY  - JOUR
AU  - Milanović, Desanka
AU  - Pešić, Vesna
AU  - Lončarević-Vasiljković, Nataša
AU  - Avramović, Vladimir
AU  - Tešić, Vesna
AU  - Jevtović-Todorović, Vesna
AU  - Kanazir, Selma
AU  - Ruždijić, Sabera
PY  - 2017
UR  - http://link.springer.com/10.1007/s12640-017-9730-0
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2741
AB  - Propofol is a general anesthetic commonly used in pediatric clinical practices. Experimental findings demonstrate that anesthetics induce widespread apoptosis and cognitive decline in a developing brain. Although anesthesia-mediated neurotoxicity is the most prominent during intense period of synaptogenesis, the effects of an early anesthesia exposure on the synapses are not well understood. The aim of this study was to examine the effects of neonatal propofol anesthesia on the expression of key proteins that participate in synaptogenesis and synaptic plasticity and to evaluate long-term neurobehavioral abnormalities in the mature adult brain. Propofol-injected 7-day-old rats were maintained under 2-, 4-, and 6-h-long anesthesia and sacrificed 0, 4, 16, and 24 h after the termination of each exposure. We showed that propofol anesthesia strongly influenced spatiotemporal expression and/or proteolytic processing of crucial presynaptic (GAP-43, synaptophysin, α-synuclein), trans-synaptic (N-cadherin), and postsynaptic (drebrin, MAP-2) proteins in the cortex and thalamus. An overall decrease of synaptophysin, α-synuclein, N-cadherin, and drebrin indicated impaired function and structure of the synaptic contacts immediately after anesthesia cessation. GAP-43 and MAP-2 adult and juvenile isoforms are upregulated following anesthesia, suggesting compensatory mechanism in the maintaining of the structural integrity and stabilization of developing axons and dendritic arbors. Neonatal propofol exposure significantly altered spontaneous motor activity (increased stereotypic/repetitive movements) and changed emotional behavior (reduced anxiety-like response) in the adulthood, 6 months later. These findings suggest that propofol anesthesia is synaptotoxic in the developing brain, disturbing synaptic dynamics and producing neuroplastic changes permanently incorporated into existing networks with long-lasting functional consequences.
T2  - Neurotoxicity Research
T1  - Neonatal Propofol Anesthesia Changes Expression of Synaptic Plasticity Proteins and Increases Stereotypic and Anxyolitic Behavior in Adult Rats
VL  - 32
DO  - 10.1007/s12640-017-9730-0
SP  - 247
EP  - 263
ER  - 

@article{
author = "Milanović, Desanka and Pešić, Vesna and Lončarević-Vasiljković, Nataša and Avramović, Vladimir and Tešić, Vesna and Jevtović-Todorović, Vesna and Kanazir, Selma and Ruždijić, Sabera",
year = "2017",
abstract = "Propofol is a general anesthetic commonly used in pediatric clinical practices. Experimental findings demonstrate that anesthetics induce widespread apoptosis and cognitive decline in a developing brain. Although anesthesia-mediated neurotoxicity is the most prominent during intense period of synaptogenesis, the effects of an early anesthesia exposure on the synapses are not well understood. The aim of this study was to examine the effects of neonatal propofol anesthesia on the expression of key proteins that participate in synaptogenesis and synaptic plasticity and to evaluate long-term neurobehavioral abnormalities in the mature adult brain. Propofol-injected 7-day-old rats were maintained under 2-, 4-, and 6-h-long anesthesia and sacrificed 0, 4, 16, and 24 h after the termination of each exposure. We showed that propofol anesthesia strongly influenced spatiotemporal expression and/or proteolytic processing of crucial presynaptic (GAP-43, synaptophysin, α-synuclein), trans-synaptic (N-cadherin), and postsynaptic (drebrin, MAP-2) proteins in the cortex and thalamus. An overall decrease of synaptophysin, α-synuclein, N-cadherin, and drebrin indicated impaired function and structure of the synaptic contacts immediately after anesthesia cessation. GAP-43 and MAP-2 adult and juvenile isoforms are upregulated following anesthesia, suggesting compensatory mechanism in the maintaining of the structural integrity and stabilization of developing axons and dendritic arbors. Neonatal propofol exposure significantly altered spontaneous motor activity (increased stereotypic/repetitive movements) and changed emotional behavior (reduced anxiety-like response) in the adulthood, 6 months later. These findings suggest that propofol anesthesia is synaptotoxic in the developing brain, disturbing synaptic dynamics and producing neuroplastic changes permanently incorporated into existing networks with long-lasting functional consequences.",
journal = "Neurotoxicity Research",
title = "Neonatal Propofol Anesthesia Changes Expression of Synaptic Plasticity Proteins and Increases Stereotypic and Anxyolitic Behavior in Adult Rats",
volume = "32",
doi = "10.1007/s12640-017-9730-0",
pages = "247-263"
}

Milanović, D., Pešić, V., Lončarević-Vasiljković, N., Avramović, V., Tešić, V., Jevtović-Todorović, V., Kanazir, S.,& Ruždijić, S.. (2017). Neonatal Propofol Anesthesia Changes Expression of Synaptic Plasticity Proteins and Increases Stereotypic and Anxyolitic Behavior in Adult Rats. in Neurotoxicity Research, 32, 247-263.
https://doi.org/10.1007/s12640-017-9730-0

Milanović D, Pešić V, Lončarević-Vasiljković N, Avramović V, Tešić V, Jevtović-Todorović V, Kanazir S, Ruždijić S. Neonatal Propofol Anesthesia Changes Expression of Synaptic Plasticity Proteins and Increases Stereotypic and Anxyolitic Behavior in Adult Rats. in Neurotoxicity Research. 2017;32:247-263.
doi:10.1007/s12640-017-9730-0 .

Milanović, Desanka, Pešić, Vesna, Lončarević-Vasiljković, Nataša, Avramović, Vladimir, Tešić, Vesna, Jevtović-Todorović, Vesna, Kanazir, Selma, Ruždijić, Sabera, "Neonatal Propofol Anesthesia Changes Expression of Synaptic Plasticity Proteins and Increases Stereotypic and Anxyolitic Behavior in Adult Rats" in Neurotoxicity Research, 32 (2017):247-263,
https://doi.org/10.1007/s12640-017-9730-0 . .

TY  - JOUR
AU  - Tešić, Vesna
AU  - Perović, Milka
AU  - Zaletel, Ivan
AU  - Jovanović, Mirna
AU  - Puškaš, Nela
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0531556517302462
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2839
AB  - The administration of dexamethasone, a synthetic glucocorticoid receptor agonist, has been reported to modulate cognitive performance in both animals and humans. In the present study, we demonstrate the effects of a single high dose of dexamethasone on the expression and distribution of synaptic plasticity-related proteins, growth-associated protein-43 (GAP-43) and synaptophysin, in the hippocampus of 6-, 12-, 18- and 24-month-old rats. Acute dexamethasone treatment significantly altered the expression of GAP-43 at the posttranslational level by modulating the levels of phosphorylated GAP-43 and proteolytic GAP-43-3 fragment. The effect was the most pronounced in the hippocampi of the aged animals. The total GAP-43 protein was increased only in 24-month-old dexamethasone-treated animals, and was concomitant with a decrease in calpain-mediated proteolysis. Moreover, by introducing the gray level co-occurrence matrix method, a form of texture analysis, we were able to reveal the subtle differences in the expression pattern of both GAP-43 and synaptophysin in the hippocampal subfields that were not detected by Western blot analysis alone. Therefore, the current study demonstrates, through a novel combined approach, that dexamethasone treatment significantly affects both GAP-43 and synaptophysin protein expression in the hippocampus of aged rats.
T2  - Experimental Gerontology
T1  - A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats
VL  - 98
DO  - 10.1016/j.exger.2017.08.010
SP  - 62
EP  - 69
ER  - 

@article{
author = "Tešić, Vesna and Perović, Milka and Zaletel, Ivan and Jovanović, Mirna and Puškaš, Nela and Ruždijić, Sabera and Kanazir, Selma",
year = "2017",
abstract = "The administration of dexamethasone, a synthetic glucocorticoid receptor agonist, has been reported to modulate cognitive performance in both animals and humans. In the present study, we demonstrate the effects of a single high dose of dexamethasone on the expression and distribution of synaptic plasticity-related proteins, growth-associated protein-43 (GAP-43) and synaptophysin, in the hippocampus of 6-, 12-, 18- and 24-month-old rats. Acute dexamethasone treatment significantly altered the expression of GAP-43 at the posttranslational level by modulating the levels of phosphorylated GAP-43 and proteolytic GAP-43-3 fragment. The effect was the most pronounced in the hippocampi of the aged animals. The total GAP-43 protein was increased only in 24-month-old dexamethasone-treated animals, and was concomitant with a decrease in calpain-mediated proteolysis. Moreover, by introducing the gray level co-occurrence matrix method, a form of texture analysis, we were able to reveal the subtle differences in the expression pattern of both GAP-43 and synaptophysin in the hippocampal subfields that were not detected by Western blot analysis alone. Therefore, the current study demonstrates, through a novel combined approach, that dexamethasone treatment significantly affects both GAP-43 and synaptophysin protein expression in the hippocampus of aged rats.",
journal = "Experimental Gerontology",
title = "A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats",
volume = "98",
doi = "10.1016/j.exger.2017.08.010",
pages = "62-69"
}

Tešić, V., Perović, M., Zaletel, I., Jovanović, M., Puškaš, N., Ruždijić, S.,& Kanazir, S.. (2017). A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats. in Experimental Gerontology, 98, 62-69.
https://doi.org/10.1016/j.exger.2017.08.010

Tešić V, Perović M, Zaletel I, Jovanović M, Puškaš N, Ruždijić S, Kanazir S. A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats. in Experimental Gerontology. 2017;98:62-69.
doi:10.1016/j.exger.2017.08.010 .

Tešić, Vesna, Perović, Milka, Zaletel, Ivan, Jovanović, Mirna, Puškaš, Nela, Ruždijić, Sabera, Kanazir, Selma, "A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats" in Experimental Gerontology, 98 (2017):62-69,
https://doi.org/10.1016/j.exger.2017.08.010 . .

TY  - JOUR
AU  - Milošev, Milorad Z
AU  - Jakovljević, Katarina
AU  - Joksović, Milan D
AU  - Stanojković, Tatjana
AU  - Matić, Ivana Z
AU  - Perović, Milka
AU  - Tešić, Vesna
AU  - Kanazir, Selma
AU  - Mladenović, Milan
AU  - Rodić, Marko V
AU  - Leovac, Vukadin M
AU  - Trifunović, Snežana
AU  - Marković, Violeta
PY  - 2017
UR  - http://doi.wiley.com/10.1111/cbdd.12920
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27933733
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2753
AB  - A series of 18 novel N-Mannich bases derived from 5-adamantyl-1,2,4-triazole-3-thione was synthesized and characterized using NMR spectroscopy and X-ray diffraction technique. All derivatives were evaluated for their anticancer potential against four human cancer cell lines. Several tested compounds exerted good cytotoxic activities on K562 and HL-60 cell lines, along with pronounced selectivity, showing lower cytotoxicity against normal fibroblasts MRC-5 compared to cancer cells. The effects of compounds 5b, 5e, and 5j on the cell cycle were investigated by flow cytometric analysis. It was found that these compounds cause the accumulation of cells in the subG1 and G1 phases of the cell cycle and induce caspase-dependent apoptosis, while the anti-angiogenic effects of 5b, 5e, and 5j have been confirmed in EA.hy926 cells using a tube formation assay. Further, the interaction of Bax protein with compound 5b was investigated by means of molecular modeling, applying the combined molecular docking/molecular dynamics approach.
T2  - Chemical Biology & Drug Design
T1  - Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies
IS  - 6
VL  - 89
DO  - 10.1111/cbdd.12920
SP  - 943
EP  - 952
ER  - 

@article{
author = "Milošev, Milorad Z and Jakovljević, Katarina and Joksović, Milan D and Stanojković, Tatjana and Matić, Ivana Z and Perović, Milka and Tešić, Vesna and Kanazir, Selma and Mladenović, Milan and Rodić, Marko V and Leovac, Vukadin M and Trifunović, Snežana and Marković, Violeta",
year = "2017",
abstract = "A series of 18 novel N-Mannich bases derived from 5-adamantyl-1,2,4-triazole-3-thione was synthesized and characterized using NMR spectroscopy and X-ray diffraction technique. All derivatives were evaluated for their anticancer potential against four human cancer cell lines. Several tested compounds exerted good cytotoxic activities on K562 and HL-60 cell lines, along with pronounced selectivity, showing lower cytotoxicity against normal fibroblasts MRC-5 compared to cancer cells. The effects of compounds 5b, 5e, and 5j on the cell cycle were investigated by flow cytometric analysis. It was found that these compounds cause the accumulation of cells in the subG1 and G1 phases of the cell cycle and induce caspase-dependent apoptosis, while the anti-angiogenic effects of 5b, 5e, and 5j have been confirmed in EA.hy926 cells using a tube formation assay. Further, the interaction of Bax protein with compound 5b was investigated by means of molecular modeling, applying the combined molecular docking/molecular dynamics approach.",
journal = "Chemical Biology & Drug Design",
title = "Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies",
number = "6",
volume = "89",
doi = "10.1111/cbdd.12920",
pages = "943-952"
}

Milošev, M. Z., Jakovljević, K., Joksović, M. D., Stanojković, T., Matić, I. Z., Perović, M., Tešić, V., Kanazir, S., Mladenović, M., Rodić, M. V., Leovac, V. M., Trifunović, S.,& Marković, V.. (2017). Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies. in Chemical Biology & Drug Design, 89(6), 943-952.
https://doi.org/10.1111/cbdd.12920

Milošev MZ, Jakovljević K, Joksović MD, Stanojković T, Matić IZ, Perović M, Tešić V, Kanazir S, Mladenović M, Rodić MV, Leovac VM, Trifunović S, Marković V. Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies. in Chemical Biology & Drug Design. 2017;89(6):943-952.
doi:10.1111/cbdd.12920 .

Milošev, Milorad Z, Jakovljević, Katarina, Joksović, Milan D, Stanojković, Tatjana, Matić, Ivana Z, Perović, Milka, Tešić, Vesna, Kanazir, Selma, Mladenović, Milan, Rodić, Marko V, Leovac, Vukadin M, Trifunović, Snežana, Marković, Violeta, "Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies" in Chemical Biology & Drug Design, 89, no. 6 (2017):943-952,
https://doi.org/10.1111/cbdd.12920 . .

TY  - CONF
AU  - Avramović, Vladimir
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Babić, Nikolina
AU  - Jović, Milena
AU  - Perović, Milka
AU  - Tešić, Vesna
AU  - Ivković, Sanja
AU  - Kanazir, Selma
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5829
AB  - Clinico-pathological features of Alzheimer's disease (AD) include memory loss, cognitive impairment, depression and social isolation. Epidemiological studies show a positive role of omega-3 poly-unsaturated fatty acids (n-3 PU FA) on prevention and mitigation of mild AD pathology. The 5xFAD mouse model used in this study bears five mutations linked to familiar forms of AD and recapitulates in a few months the main features of AD. This study was aimed to evaluate the effect of pre- and peri-natal omega-3 fatty acids supplementation on cognitive and non-cognitive behavior of 5xFAD mice . The fish oil, an abundant source of n-3 PUFA, was supplemented via oral gavages five days per week to dames throughout whole pregnancy and lactation. Six-month old offsprings were subjected to a battery of behavioral tests in order to assess typical rodent behavior, general locomotor activity, memory, depressive-like and social behavior. We have observed that the fish oil-supplemented 5xFAD offspring showed no changes in tests assessing typical rodent behavior, such as marble and nesting test, in comparison with 5XFAD control mice. When assessing depression and anxiety like behavior in a light-dark box, forced swim and open field behavioral tests significant changes were observed only between non-transgenic and transgenic mice. However, in three chambers test, used for assessing social behavior, fish oil- treated 5xFAD mice showed a significant increase in sociability, evaluated by increased time spent in compartment with, a mouse vs. empty compartment. Moreover, when the social novelty was tested through the introduction of a new mouse, the same trend was observed. This study shows that the pre- and peri-natal fish oil supplementation in 5xFAD mice can exert long-lasting effects inducing the significant improvement in some of the behavioral aspects of AD pathology in the adult offspring.
PB  - Belgrade: Serbian Nutrition Society
C3  - Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia
T1  - Fish oil supplementation during pre- and peri-natal period: Behavioral phenotyping of transgenic mouse model of Alzheimer’s disease
SP  - 257
EP  - 258
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5829
ER  - 

@conference{
author = "Avramović, Vladimir and Milanović, Desanka and Brkić, Marjana and Babić, Nikolina and Jović, Milena and Perović, Milka and Tešić, Vesna and Ivković, Sanja and Kanazir, Selma",
year = "2016",
abstract = "Clinico-pathological features of Alzheimer's disease (AD) include memory loss, cognitive impairment, depression and social isolation. Epidemiological studies show a positive role of omega-3 poly-unsaturated fatty acids (n-3 PU FA) on prevention and mitigation of mild AD pathology. The 5xFAD mouse model used in this study bears five mutations linked to familiar forms of AD and recapitulates in a few months the main features of AD. This study was aimed to evaluate the effect of pre- and peri-natal omega-3 fatty acids supplementation on cognitive and non-cognitive behavior of 5xFAD mice . The fish oil, an abundant source of n-3 PUFA, was supplemented via oral gavages five days per week to dames throughout whole pregnancy and lactation. Six-month old offsprings were subjected to a battery of behavioral tests in order to assess typical rodent behavior, general locomotor activity, memory, depressive-like and social behavior. We have observed that the fish oil-supplemented 5xFAD offspring showed no changes in tests assessing typical rodent behavior, such as marble and nesting test, in comparison with 5XFAD control mice. When assessing depression and anxiety like behavior in a light-dark box, forced swim and open field behavioral tests significant changes were observed only between non-transgenic and transgenic mice. However, in three chambers test, used for assessing social behavior, fish oil- treated 5xFAD mice showed a significant increase in sociability, evaluated by increased time spent in compartment with, a mouse vs. empty compartment. Moreover, when the social novelty was tested through the introduction of a new mouse, the same trend was observed. This study shows that the pre- and peri-natal fish oil supplementation in 5xFAD mice can exert long-lasting effects inducing the significant improvement in some of the behavioral aspects of AD pathology in the adult offspring.",
publisher = "Belgrade: Serbian Nutrition Society",
journal = "Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia",
title = "Fish oil supplementation during pre- and peri-natal period: Behavioral phenotyping of transgenic mouse model of Alzheimer’s disease",
pages = "257-258",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5829"
}

Avramović, V., Milanović, D., Brkić, M., Babić, N., Jović, M., Perović, M., Tešić, V., Ivković, S.,& Kanazir, S.. (2016). Fish oil supplementation during pre- and peri-natal period: Behavioral phenotyping of transgenic mouse model of Alzheimer’s disease. in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia
Belgrade: Serbian Nutrition Society., 257-258.
https://hdl.handle.net/21.15107/rcub_ibiss_5829

Avramović V, Milanović D, Brkić M, Babić N, Jović M, Perović M, Tešić V, Ivković S, Kanazir S. Fish oil supplementation during pre- and peri-natal period: Behavioral phenotyping of transgenic mouse model of Alzheimer’s disease. in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia. 2016;:257-258.
https://hdl.handle.net/21.15107/rcub_ibiss_5829 .

Avramović, Vladimir, Milanović, Desanka, Brkić, Marjana, Babić, Nikolina, Jović, Milena, Perović, Milka, Tešić, Vesna, Ivković, Sanja, Kanazir, Selma, "Fish oil supplementation during pre- and peri-natal period: Behavioral phenotyping of transgenic mouse model of Alzheimer’s disease" in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia (2016):257-258,
https://hdl.handle.net/21.15107/rcub_ibiss_5829 .

TY  - JOUR
AU  - Lončarević-Vasiljković, Nataša
AU  - Milanović, Desanka
AU  - Pešić, Vesna
AU  - Tešić, Vesna
AU  - Brkić, Marjana
AU  - Lazić, Divna
AU  - Avramović, Vladimir
AU  - Kanazir, Selma
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5437
AB  - Subsequent pathological events occurring in the brain after TBI, referred to as secondary injury, continue to damage surrounding tissue resulting in substantial neuronal loss. Using an animal model of TBI we examined the effect of dietary restriction (DR) on the neuroapoptosis and Bcl-2 family genes as the main regulators of the intrinsic apoptotic pathway. Bcl-2, Bcl-xl and Bax mRNA and protein expression in the ipsilateral cortex of adult Wistar rats exposed to DR before TBI were studied from 2 to 28 days post injury. Our results showed that DR suppressed neuroapoptosis and promoted significant upregulation of antiapoptotic Bcl-2 and Bcl-xl mRNAs in the ipsilateral cortex following injury. Expression of the proapoptotic Bax gene increased in ad libitum (AL) fed rats but remained unchanged in rats exposed to DR. Although the expression of Bcl-2, Bcl-xl and Bax proteins was changed in a similar manner in both experimental groups, DR promoted a continuous increase in the Bcl-2:Bax protein ratio throughout the recovery period. Together with our previous finding that DR mediates inhibition of the extrinsic apoptotic pathway the present work reveals that modulation of the intrinsic pathway contributes to the beneficial effect of DR in brain injury. These findings provide new insight into the effects of DR on pro-survival signaling after injury, lending further support to its neuroprotective effect.
PB  - England : Elsevier
T2  - Neurochemistry International
T1  - Dietary restriction suppresses apoptotic cell death, promotes Bcl-2 and Bcl-xl mRNA expression and increases the Bcl-2/Bax protein ratio in the rat cortex after cortical injury.
VL  - 96
DO  - 10.1016/j.neuint.2016.02.017
SP  - 69
EP  - 76
ER  - 

@article{
author = "Lončarević-Vasiljković, Nataša and Milanović, Desanka and Pešić, Vesna and Tešić, Vesna and Brkić, Marjana and Lazić, Divna and Avramović, Vladimir and Kanazir, Selma",
year = "2016",
abstract = "Subsequent pathological events occurring in the brain after TBI, referred to as secondary injury, continue to damage surrounding tissue resulting in substantial neuronal loss. Using an animal model of TBI we examined the effect of dietary restriction (DR) on the neuroapoptosis and Bcl-2 family genes as the main regulators of the intrinsic apoptotic pathway. Bcl-2, Bcl-xl and Bax mRNA and protein expression in the ipsilateral cortex of adult Wistar rats exposed to DR before TBI were studied from 2 to 28 days post injury. Our results showed that DR suppressed neuroapoptosis and promoted significant upregulation of antiapoptotic Bcl-2 and Bcl-xl mRNAs in the ipsilateral cortex following injury. Expression of the proapoptotic Bax gene increased in ad libitum (AL) fed rats but remained unchanged in rats exposed to DR. Although the expression of Bcl-2, Bcl-xl and Bax proteins was changed in a similar manner in both experimental groups, DR promoted a continuous increase in the Bcl-2:Bax protein ratio throughout the recovery period. Together with our previous finding that DR mediates inhibition of the extrinsic apoptotic pathway the present work reveals that modulation of the intrinsic pathway contributes to the beneficial effect of DR in brain injury. These findings provide new insight into the effects of DR on pro-survival signaling after injury, lending further support to its neuroprotective effect.",
publisher = "England : Elsevier",
journal = "Neurochemistry International",
title = "Dietary restriction suppresses apoptotic cell death, promotes Bcl-2 and Bcl-xl mRNA expression and increases the Bcl-2/Bax protein ratio in the rat cortex after cortical injury.",
volume = "96",
doi = "10.1016/j.neuint.2016.02.017",
pages = "69-76"
}

Lončarević-Vasiljković, N., Milanović, D., Pešić, V., Tešić, V., Brkić, M., Lazić, D., Avramović, V.,& Kanazir, S.. (2016). Dietary restriction suppresses apoptotic cell death, promotes Bcl-2 and Bcl-xl mRNA expression and increases the Bcl-2/Bax protein ratio in the rat cortex after cortical injury.. in Neurochemistry International
England : Elsevier., 96, 69-76.
https://doi.org/10.1016/j.neuint.2016.02.017

Lončarević-Vasiljković N, Milanović D, Pešić V, Tešić V, Brkić M, Lazić D, Avramović V, Kanazir S. Dietary restriction suppresses apoptotic cell death, promotes Bcl-2 and Bcl-xl mRNA expression and increases the Bcl-2/Bax protein ratio in the rat cortex after cortical injury.. in Neurochemistry International. 2016;96:69-76.
doi:10.1016/j.neuint.2016.02.017 .

Lončarević-Vasiljković, Nataša, Milanović, Desanka, Pešić, Vesna, Tešić, Vesna, Brkić, Marjana, Lazić, Divna, Avramović, Vladimir, Kanazir, Selma, "Dietary restriction suppresses apoptotic cell death, promotes Bcl-2 and Bcl-xl mRNA expression and increases the Bcl-2/Bax protein ratio in the rat cortex after cortical injury." in Neurochemistry International, 96 (2016):69-76,
https://doi.org/10.1016/j.neuint.2016.02.017 . .

TY  - THES
AU  - Tešić, Vesna
PY  - 2015
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=2621
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:10711/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024990130
UR  - http://nardus.mpn.gov.rs/123456789/4928
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2421
AB  - Dugotrajna restrikcija hrane produžava životni vek i odlaže pojavu mnogih bolesti koje sejavljaju sa starenjem. Brojni literaturni podaci ukazuju da kod životinja kojima je unos hranesmanjen izostaje karakterističan starosno-zavistan pad u kognitivnim funkcijama, međutim,mehanizam ovakvog neuroprotektivnog dejstva restrikcije hrane nije u potpunosti razjašnjen. Uisto vreme, kod životinja na dugotrajnoj restrikciji hrane se povećava nivo kortikosterona uplazmi što ukazuje da ovaj tretman deluje kao blagi stresor. Do sada nije ispitivano do kakvihpromena u signalnom putu glukokortikoida dovodi smanjeni unos hrane tokom starenja u mozgu.Centralno polje istraživanja procesa starenja neizbežno podrazumeva ispitivanje promena dokojih dolazi u korteksu i hipokampusu, strukturama mozga koje su ključne za kognitivnefunkcije. Sa druge strane, iste strukture učestvuju u regulaciji HHA ose kao važni regionidelovanja negativne povratne sprege.Cilj doktorske disertacije je da se ispita uloga signalnog puta glukokortikoida u korteksu ihipokampusu pacova tokom starenja i pod uticajem dugotrajne restrikcije hrane.Eksperimentalne životinje (mužjaci pacova soja Wistar) starosti 6 meseci su podeljene u dvegrupe. Prva grupa, označena kao ad libitum (AL), je imala neograničen pristup hrani, dok jedruga grupa (označena kao DR) podvrgnuta režimu redukovane ishrane koji je podrazumevaodobijanje 100% dnevnog unosa hrane AL životinja svakog drugog dana. Životinje su analiziranekada su dostigle starost od 18 i 24 meseca. Životinje stare 6 meseci su predstavljale kontrolnugrupu. Glukokortikoidna signalizacija u ispitivanim strukturama je praćena na prereceptorskomnivou, kao i na nivou ekspresije i aktivacije glukokortikoidnog receptora primenom imunoesej,imunoblot i PCR metoda, kao i imunohistohemijske analize.Rezultati doktorske disertacije pokazuju da tokom starenja dolazi do povećanjakoncentracije kortikosterona u korteksu pacova, dok dugotrajna restrikcija hrane povećava nivokortikosterona u obe ispitivane strukture. Utvrđeno je da nivo ključnog enzima koji u moždanomtkivu reguliše dostupnost kortikosterona za receptore, 11β-HSD1, raste u hipokampusu pacovakoji imaju neograničen pristup hrani. Tretman dugotrajnom restrikcijom hrane dovodi dopovećanja nivoa 11β-HSD1 u korteksu, dok u hipokampusu izostaje efekat na nivo ovog enzima...
AB  - Moderate restriction in food intake (dietary restriction, DR) extends the life-span anddelays the onset of many age-related diseases. In addition, food-restricted animals exhibitattenuated cognitive deficits during aging as they perform better in learning and memory tasksthan their ad libitum fed counterparts. At the same time, the animals on chronic food restrictionhave elevated levels of plasma corticosterone, which implies that this treatment could beconsidered as a mild stressor. So far, there are no literature data regarding the effects of foodrestriction on glucocorticoid signaling in the brain during aging and the mechanisms by whichDR exhibits its neuroprotective effects are poorly understood. Two brain regions of interest arecortex and hippocampus, regions particularly prone to age-related changes. The same regions arealso involved in cognition and regulation of the hypothalamic–pituitary–adrenal (HPA) axisactivity.The aim of the present study was to evaluate the effect of long-term food restriction onglucocorticoid signaling pathway in the cortex and hippocampus of rats during aging.Experimental animals (male Wistar rats, 6 months old) were divided into two groups. The ALgroup was fed ad libitum, whereas the food restricted group (DR) received 100% of the meandaily intake of the AL animals every other day. The animals were examined at the age of 18 and24 months. Six-month-old animals were used as a control. Glucocorticoid signaling in specificbrain regions was examined at the pre-receptor level, as well as regarding the level of expressionand glucocorticoid receptor activity by using immunoassays, Western blot, PCR andimmunohistochemistry.The results of this study demonstrate an age-related increase in cortical corticosteroneconcentration. The increase in the hormone level was further detected in DR animals, in bothbrain regions examined. However, 11β-HSD1, a key enzyme in brain tissue that regulates theavailability of corticosterone to its receptors, was increased only in the hippocampi of AL ratsand in the cortex of rats subjected to the long-term food restriction. The most pronouncedchanges in the level of glucocorticoid receptor during aging were observed in the hippocampi of AL animals...
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Uticaj dugotrajne restrikcije hrane na ekspresiju glukokortikoidnog receptora u prednjem mozgu pacova tokom starenja
T1  - The effect of long-term food restriction on te expression of glucocorticoid receptor in the rat forebrain during aging
SP  - 1
EP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_nardus_4928
ER  - 

@phdthesis{
author = "Tešić, Vesna",
year = "2015",
abstract = "Dugotrajna restrikcija hrane produžava životni vek i odlaže pojavu mnogih bolesti koje sejavljaju sa starenjem. Brojni literaturni podaci ukazuju da kod životinja kojima je unos hranesmanjen izostaje karakterističan starosno-zavistan pad u kognitivnim funkcijama, međutim,mehanizam ovakvog neuroprotektivnog dejstva restrikcije hrane nije u potpunosti razjašnjen. Uisto vreme, kod životinja na dugotrajnoj restrikciji hrane se povećava nivo kortikosterona uplazmi što ukazuje da ovaj tretman deluje kao blagi stresor. Do sada nije ispitivano do kakvihpromena u signalnom putu glukokortikoida dovodi smanjeni unos hrane tokom starenja u mozgu.Centralno polje istraživanja procesa starenja neizbežno podrazumeva ispitivanje promena dokojih dolazi u korteksu i hipokampusu, strukturama mozga koje su ključne za kognitivnefunkcije. Sa druge strane, iste strukture učestvuju u regulaciji HHA ose kao važni regionidelovanja negativne povratne sprege.Cilj doktorske disertacije je da se ispita uloga signalnog puta glukokortikoida u korteksu ihipokampusu pacova tokom starenja i pod uticajem dugotrajne restrikcije hrane.Eksperimentalne životinje (mužjaci pacova soja Wistar) starosti 6 meseci su podeljene u dvegrupe. Prva grupa, označena kao ad libitum (AL), je imala neograničen pristup hrani, dok jedruga grupa (označena kao DR) podvrgnuta režimu redukovane ishrane koji je podrazumevaodobijanje 100% dnevnog unosa hrane AL životinja svakog drugog dana. Životinje su analiziranekada su dostigle starost od 18 i 24 meseca. Životinje stare 6 meseci su predstavljale kontrolnugrupu. Glukokortikoidna signalizacija u ispitivanim strukturama je praćena na prereceptorskomnivou, kao i na nivou ekspresije i aktivacije glukokortikoidnog receptora primenom imunoesej,imunoblot i PCR metoda, kao i imunohistohemijske analize.Rezultati doktorske disertacije pokazuju da tokom starenja dolazi do povećanjakoncentracije kortikosterona u korteksu pacova, dok dugotrajna restrikcija hrane povećava nivokortikosterona u obe ispitivane strukture. Utvrđeno je da nivo ključnog enzima koji u moždanomtkivu reguliše dostupnost kortikosterona za receptore, 11β-HSD1, raste u hipokampusu pacovakoji imaju neograničen pristup hrani. Tretman dugotrajnom restrikcijom hrane dovodi dopovećanja nivoa 11β-HSD1 u korteksu, dok u hipokampusu izostaje efekat na nivo ovog enzima..., Moderate restriction in food intake (dietary restriction, DR) extends the life-span anddelays the onset of many age-related diseases. In addition, food-restricted animals exhibitattenuated cognitive deficits during aging as they perform better in learning and memory tasksthan their ad libitum fed counterparts. At the same time, the animals on chronic food restrictionhave elevated levels of plasma corticosterone, which implies that this treatment could beconsidered as a mild stressor. So far, there are no literature data regarding the effects of foodrestriction on glucocorticoid signaling in the brain during aging and the mechanisms by whichDR exhibits its neuroprotective effects are poorly understood. Two brain regions of interest arecortex and hippocampus, regions particularly prone to age-related changes. The same regions arealso involved in cognition and regulation of the hypothalamic–pituitary–adrenal (HPA) axisactivity.The aim of the present study was to evaluate the effect of long-term food restriction onglucocorticoid signaling pathway in the cortex and hippocampus of rats during aging.Experimental animals (male Wistar rats, 6 months old) were divided into two groups. The ALgroup was fed ad libitum, whereas the food restricted group (DR) received 100% of the meandaily intake of the AL animals every other day. The animals were examined at the age of 18 and24 months. Six-month-old animals were used as a control. Glucocorticoid signaling in specificbrain regions was examined at the pre-receptor level, as well as regarding the level of expressionand glucocorticoid receptor activity by using immunoassays, Western blot, PCR andimmunohistochemistry.The results of this study demonstrate an age-related increase in cortical corticosteroneconcentration. The increase in the hormone level was further detected in DR animals, in bothbrain regions examined. However, 11β-HSD1, a key enzyme in brain tissue that regulates theavailability of corticosterone to its receptors, was increased only in the hippocampi of AL ratsand in the cortex of rats subjected to the long-term food restriction. The most pronouncedchanges in the level of glucocorticoid receptor during aging were observed in the hippocampi of AL animals...",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Uticaj dugotrajne restrikcije hrane na ekspresiju glukokortikoidnog receptora u prednjem mozgu pacova tokom starenja, The effect of long-term food restriction on te expression of glucocorticoid receptor in the rat forebrain during aging",
pages = "1-101",
url = "https://hdl.handle.net/21.15107/rcub_nardus_4928"
}

Tešić, V.. (2015). Uticaj dugotrajne restrikcije hrane na ekspresiju glukokortikoidnog receptora u prednjem mozgu pacova tokom starenja. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-101.
https://hdl.handle.net/21.15107/rcub_nardus_4928

Tešić V. Uticaj dugotrajne restrikcije hrane na ekspresiju glukokortikoidnog receptora u prednjem mozgu pacova tokom starenja. in University of Belgrade, Faculty of Biology. 2015;:1-101.
https://hdl.handle.net/21.15107/rcub_nardus_4928 .

Tešić, Vesna, "Uticaj dugotrajne restrikcije hrane na ekspresiju glukokortikoidnog receptora u prednjem mozgu pacova tokom starenja" in University of Belgrade, Faculty of Biology (2015):1-101,
https://hdl.handle.net/21.15107/rcub_nardus_4928 .