TY  - JOUR
AU  - Devrnja, Nina
AU  - Anđelković, Boban
AU  - Ljujić, Jovana
AU  - Ćosić, Tatjana
AU  - Stupar, Sofija
AU  - Milutinović, Milica
AU  - Savić, Jelena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6632
AB  - β-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils
(FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation
method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and
monoterpenoids were the dominant chemical groups in total FEO, while in BEO, these two groups
occurred along with sesquiterpenes and sesquiterpenoids. The presence of dominant compounds
from both EOs was validated using the FT-IR spectra of ICs, which indicated successful complexation.
Analyses conducted using SPME/GC-MS showed the continuous emission of volatiles over 24 h from
both ICs. Under SEM, particles of both ICs appeared to have a rectangular or rhomboid morphology
and few aggregates. The insecticidal properties of EOs and ICs with β-CD were tested on the
Colorado potato beetle (CPB) as a model pest. The inclusion complex of β-CD with FEO altered the
developmental dynamic and body mass of the CPB. The initial increase in the proteolytic activity of
CPB larvae fed with potato plants sprayed with ICs was not maintained for long, and the proteolytic
efficacy of treated larvae remained in line with that of the control larvae. Future investigations will
focus on manipulating the volume of EOs used and the treatment duration for optimal efficacy and
potential application.
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation
IS  - 3
VL  - 14
DO  - 10.3390/biom14030353
SP  - 353
ER  - 

@article{
author = "Devrnja, Nina and Anđelković, Boban and Ljujić, Jovana and Ćosić, Tatjana and Stupar, Sofija and Milutinović, Milica and Savić, Jelena",
year = "2024",
abstract = "β-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils
(FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation
method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and
monoterpenoids were the dominant chemical groups in total FEO, while in BEO, these two groups
occurred along with sesquiterpenes and sesquiterpenoids. The presence of dominant compounds
from both EOs was validated using the FT-IR spectra of ICs, which indicated successful complexation.
Analyses conducted using SPME/GC-MS showed the continuous emission of volatiles over 24 h from
both ICs. Under SEM, particles of both ICs appeared to have a rectangular or rhomboid morphology
and few aggregates. The insecticidal properties of EOs and ICs with β-CD were tested on the
Colorado potato beetle (CPB) as a model pest. The inclusion complex of β-CD with FEO altered the
developmental dynamic and body mass of the CPB. The initial increase in the proteolytic activity of
CPB larvae fed with potato plants sprayed with ICs was not maintained for long, and the proteolytic
efficacy of treated larvae remained in line with that of the control larvae. Future investigations will
focus on manipulating the volume of EOs used and the treatment duration for optimal efficacy and
potential application.",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation",
number = "3",
volume = "14",
doi = "10.3390/biom14030353",
pages = "353"
}

Devrnja, N., Anđelković, B., Ljujić, J., Ćosić, T., Stupar, S., Milutinović, M.,& Savić, J.. (2024). Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation. in Biomolecules
Basel: MDPI., 14(3), 353.
https://doi.org/10.3390/biom14030353

Devrnja N, Anđelković B, Ljujić J, Ćosić T, Stupar S, Milutinović M, Savić J. Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation. in Biomolecules. 2024;14(3):353.
doi:10.3390/biom14030353 .

Devrnja, Nina, Anđelković, Boban, Ljujić, Jovana, Ćosić, Tatjana, Stupar, Sofija, Milutinović, Milica, Savić, Jelena, "Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation" in Biomolecules, 14, no. 3 (2024):353,
https://doi.org/10.3390/biom14030353 . .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6372
AB  - French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.
PB  - Springer Nature
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil
DO  - 10.1007/s00344-023-11131-8
ER  - 

@article{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2023",
abstract = "French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.",
publisher = "Springer Nature",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil",
doi = "10.1007/s00344-023-11131-8"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2023). Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation
Springer Nature..
https://doi.org/10.1007/s00344-023-11131-8

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation. 2023;.
doi:10.1007/s00344-023-11131-8 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil" in Journal of Plant Growth Regulation (2023),
https://doi.org/10.1007/s00344-023-11131-8 . .

TY  - DATA
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5388
AB  - Flower strips of French marigold are commonly used pest repellents in potato fields. However, the effect of French marigold volatiles on potato metabolism is unknown. Since it is shown that different volatiles affect phytohormone signaling pathways, it is assumed that phytohormones have an important role in response to essential oils. Thus, a microarray transcriptome analysis and annotation of differentialy expresed transcripts into different phytohormone metabolic pathways was performed to study the effects of French marigold essential oil on laboratory-grown potato phytohormone homeostasis. Obtained research data are presented in 4 csv files. Names of the files are abbreviated names of corresponding phytohormone groups. Supporting information about the data are presented in the Read Me file.
T1  - Microarray data of phytohormone related genes in potato exposed to French marigold essential oil
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5388
ER  - 

@misc{
author = "Stupar, Sofija and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2023",
abstract = "Flower strips of French marigold are commonly used pest repellents in potato fields. However, the effect of French marigold volatiles on potato metabolism is unknown. Since it is shown that different volatiles affect phytohormone signaling pathways, it is assumed that phytohormones have an important role in response to essential oils. Thus, a microarray transcriptome analysis and annotation of differentialy expresed transcripts into different phytohormone metabolic pathways was performed to study the effects of French marigold essential oil on laboratory-grown potato phytohormone homeostasis. Obtained research data are presented in 4 csv files. Names of the files are abbreviated names of corresponding phytohormone groups. Supporting information about the data are presented in the Read Me file.",
title = "Microarray data of phytohormone related genes in potato exposed to French marigold essential oil",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5388"
}

Stupar, S., Devrnja, N., Tubić, L.,& Savić, J.. (2023). Microarray data of phytohormone related genes in potato exposed to French marigold essential oil. .
https://hdl.handle.net/21.15107/rcub_ibiss_5388

Stupar S, Devrnja N, Tubić L, Savić J. Microarray data of phytohormone related genes in potato exposed to French marigold essential oil. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5388 .

Stupar, Sofija, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Microarray data of phytohormone related genes in potato exposed to French marigold essential oil" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5388 .

TY  - CONF
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5049
AB  - French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
T1  - Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5049
ER  - 

@conference{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022",
title = "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5049"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2022). Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
Belgrade: Serbian Plant Physiology Society., 76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022. 2022;:76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil" in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022 (2022):76,
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Gašić, Uroš
AU  - Šajkunić, Sanja
AU  - Cingel, Aleksandar
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/3/407
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4789
AB  - French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antiox-idant activity statistically comparable with positive control Trolox, was chosen for testing of anti-feedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.
PB  - Basel: MDPI
T2  - Plants
T1  - UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle
IS  - 3
VL  - 11
DO  - 10.3390/plants11030407
SP  - 407
ER  - 

@article{
author = "Devrnja, Nina and Gašić, Uroš and Šajkunić, Sanja and Cingel, Aleksandar and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antiox-idant activity statistically comparable with positive control Trolox, was chosen for testing of anti-feedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle",
number = "3",
volume = "11",
doi = "10.3390/plants11030407",
pages = "407"
}

Devrnja, N., Gašić, U., Šajkunić, S., Cingel, A., Stupar, S., Tubić, L.,& Savić, J.. (2022). UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle. in Plants
Basel: MDPI., 11(3), 407.
https://doi.org/10.3390/plants11030407

Devrnja N, Gašić U, Šajkunić S, Cingel A, Stupar S, Tubić L, Savić J. UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle. in Plants. 2022;11(3):407.
doi:10.3390/plants11030407 .

Devrnja, Nina, Gašić, Uroš, Šajkunić, Sanja, Cingel, Aleksandar, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, "UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle" in Plants, 11, no. 3 (2022):407,
https://doi.org/10.3390/plants11030407 . .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Stupar, Sofija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5067
AB  - Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to 
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards 
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and 
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect 
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings 
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well 
as antioxidant enzymes activities were determined spectrophotometrically in treated and control 
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to 
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The 
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93% 
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced 
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin 
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities 
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of 
further investigation.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5067
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Stupar, Sofija and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to 
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards 
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and 
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect 
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings 
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well 
as antioxidant enzymes activities were determined spectrophotometrically in treated and control 
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to 
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The 
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93% 
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced 
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin 
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities 
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of 
further investigation.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5067"
}

Smailagić, D., Dragišić Maksimović, J., Stupar, S., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_5067

Smailagić D, Dragišić Maksimović J, Stupar S, Banjac N, Ninković S, Stanišić M. Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_5067 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Stupar, Sofija, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):73,
https://hdl.handle.net/21.15107/rcub_ibiss_5067 .

TY  - CONF
AU  - Šajkunić, Sanja
AU  - Dragićević, Milan
AU  - Milutinović, Milica
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Devrnja, Nina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5050
AB  - Different stressors from the environment can prime defense and enable enhanced responses
to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the
level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown
promising prospects as novel priming inducers. Still, little is known about their potential to induce
epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula
L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to
EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal.
Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM)
DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR,
and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and
treated samples were analyzed. The average methylation level of the whole genome was 72.29%
and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly
occurred in the CG context compared to CHH and CHG. In general, hypomethylated
sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially
methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein
processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes
associated with these pathways, as well as different defense responses in potato (GO:0006952),
will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress
conditions.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - French marigold essential oil affected DNA methylation in potato
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5050
ER  - 

@conference{
author = "Šajkunić, Sanja and Dragićević, Milan and Milutinović, Milica and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena and Devrnja, Nina",
year = "2022",
abstract = "Different stressors from the environment can prime defense and enable enhanced responses
to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the
level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown
promising prospects as novel priming inducers. Still, little is known about their potential to induce
epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula
L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to
EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal.
Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM)
DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR,
and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and
treated samples were analyzed. The average methylation level of the whole genome was 72.29%
and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly
occurred in the CG context compared to CHH and CHG. In general, hypomethylated
sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially
methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein
processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes
associated with these pathways, as well as different defense responses in potato (GO:0006952),
will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress
conditions.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "French marigold essential oil affected DNA methylation in potato",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5050"
}

Šajkunić, S., Dragićević, M., Milutinović, M., Stupar, S., Tubić, L., Savić, J.,& Devrnja, N.. (2022). French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 71.
https://hdl.handle.net/21.15107/rcub_ibiss_5050

Šajkunić S, Dragićević M, Milutinović M, Stupar S, Tubić L, Savić J, Devrnja N. French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:71.
https://hdl.handle.net/21.15107/rcub_ibiss_5050 .

Šajkunić, Sanja, Dragićević, Milan, Milutinović, Milica, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, Devrnja, Nina, "French marigold essential oil affected DNA methylation in potato" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):71,
https://hdl.handle.net/21.15107/rcub_ibiss_5050 .

TY  - CONF
AU  - Šajkunić, Sanja
AU  - Dragićević, Milan
AU  - Milutinović, Milica
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Devrnja, Nina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5051
AB  - Different stressors from the environment can prime defense and enable enhanced responses to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown promising prospects as novel priming inducers. Still, little is known about their potential to induce epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM) DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and treated samples were analyzed. The average methylation level of the whole genome was 72.29% and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly occurred in the CG context compared to CHH and CHG. In general, hypomethylated sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes associated with these pathways, as well as different defense responses in potato (GO:0006952), will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress conditions.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.
T1  - French marigold essential oil affected DNA methylation in potato
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5051
ER  - 

@conference{
author = "Šajkunić, Sanja and Dragićević, Milan and Milutinović, Milica and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena and Devrnja, Nina",
year = "2022",
abstract = "Different stressors from the environment can prime defense and enable enhanced responses to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown promising prospects as novel priming inducers. Still, little is known about their potential to induce epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM) DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and treated samples were analyzed. The average methylation level of the whole genome was 72.29% and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly occurred in the CG context compared to CHH and CHG. In general, hypomethylated sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes associated with these pathways, as well as different defense responses in potato (GO:0006952), will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress conditions.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.",
title = "French marigold essential oil affected DNA methylation in potato",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5051"
}

Šajkunić, S., Dragićević, M., Milutinović, M., Stupar, S., Tubić, L., Savić, J.,& Devrnja, N.. (2022). French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5051

Šajkunić S, Dragićević M, Milutinović M, Stupar S, Tubić L, Savić J, Devrnja N. French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5051 .

Šajkunić, Sanja, Dragićević, Milan, Milutinović, Milica, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, Devrnja, Nina, "French marigold essential oil affected DNA methylation in potato" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5051 .

TY  - CONF
AU  - Savić, Jelena
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Tubić, Ljiljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5053
AB  - Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Role of glutathione in potato response to French marigold essential oil
SP  - P6
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5053
ER  - 

@conference{
author = "Savić, Jelena and Stupar, Sofija and Devrnja, Nina and Šajkunić, Sanja and Tubić, Ljiljana",
year = "2022",
abstract = "Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Role of glutathione in potato response to French marigold essential oil",
pages = "P6",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5053"
}

Savić, J., Stupar, S., Devrnja, N., Šajkunić, S.,& Tubić, L.. (2022). Role of glutathione in potato response to French marigold essential oil. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., P6.
https://hdl.handle.net/21.15107/rcub_ibiss_5053

Savić J, Stupar S, Devrnja N, Šajkunić S, Tubić L. Role of glutathione in potato response to French marigold essential oil. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:P6.
https://hdl.handle.net/21.15107/rcub_ibiss_5053 .

Savić, Jelena, Stupar, Sofija, Devrnja, Nina, Šajkunić, Sanja, Tubić, Ljiljana, "Role of glutathione in potato response to French marigold essential oil" in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):P6,
https://hdl.handle.net/21.15107/rcub_ibiss_5053 .

TY  - CONF
AU  - Savić, Jelena
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Tubić, Ljiljana
PY  - 2022
UR  - https://www.plantslo.org/wp/wp-content/uploads/2022/09/PlantsInChangingEnvironment22AbstractBook.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5052
AB  - Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Role of glutathione in potato response to French marigold essential oil
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5052
ER  - 

@conference{
author = "Savić, Jelena and Stupar, Sofija and Devrnja, Nina and Šajkunić, Sanja and Tubić, Ljiljana",
year = "2022",
abstract = "Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Role of glutathione in potato response to French marigold essential oil",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5052"
}

Savić, J., Stupar, S., Devrnja, N., Šajkunić, S.,& Tubić, L.. (2022). Role of glutathione in potato response to French marigold essential oil. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Ljubljana: Slovenian Society of Plant Biology., 17.
https://hdl.handle.net/21.15107/rcub_ibiss_5052

Savić J, Stupar S, Devrnja N, Šajkunić S, Tubić L. Role of glutathione in potato response to French marigold essential oil. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:17.
https://hdl.handle.net/21.15107/rcub_ibiss_5052 .

Savić, Jelena, Stupar, Sofija, Devrnja, Nina, Šajkunić, Sanja, Tubić, Ljiljana, "Role of glutathione in potato response to French marigold essential oil" in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):17,
https://hdl.handle.net/21.15107/rcub_ibiss_5052 .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Stanković-Jeremić, Jovana
AU  - Maksimović, Vuk
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - internal-pdf://Stupar et al. - 2021 - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf St.pdf
UR  - https://www.mdpi.com/2223-7747/10/1/172
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4147
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI AG
T2  - Plants
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
SP  - 172
ER  - 

@article{
author = "Stupar, Sofija and Dragićević, Milan and Tešević, Vele and Stanković-Jeremić, Jovana and Maksimović, Vuk and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI AG",
journal = "Plants",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172",
pages = "172"
}

Stupar, S., Dragićević, M., Tešević, V., Stanković-Jeremić, J., Maksimović, V., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants
MDPI AG., 10(1), 172.
https://doi.org/10.3390/plants10010172

Stupar S, Dragićević M, Tešević V, Stanković-Jeremić J, Maksimović V, Ćosić T, Devrnja N, Tubić L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants. 2021;10(1):172.
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragićević, Milan, Tešević, Vele, Stanković-Jeremić, Jovana, Maksimović, Vuk, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants, 10, no. 1 (2021):172,
https://doi.org/10.3390/plants10010172 . .

TY  - CONF
AU  - Stupar, Sofija
AU  - Avramović, Miloš
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5593
AB  - Potato fields pest protection is still predominantly based on synthetic pesticides, expensive in terms of monetary investment and proved to be harmful to human health and the environment. Alternatively, flower strips of French marigold (Tagetes patula L.) appearing as potent pest repellents in potato fields, offer well-adopted practice in organic farming. Up to now, the effect of French marigoldon potato metabolism, physiology, and yield has not been studied in detail. Thus, a microarray transcriptome analysis was performed to study the effect of French marigold essential oil (EO) on laboratory-grown potato, with the focus on starch metabolism pathway. After 8h of exposure to EO, 3840 potato probes were differentially expressed (fold change ≥2) compared to control plants, of which 1744 were upregulated and 2096 downregulated. We selected a set of putative genes involved in the starch metabolism pathway in plastids, and validated microarray patterns using RT-qPCR method. The expression of selected genes was analyzed in plants exposed to EO for 4, 8 and 12h . While the expression of genes involved in starch biosynthesis was at similar levels as in non-treated plants, the most of the genes responsible for gradual degradation of starch displayed significantly elevated expression with the highest levels detected after 8 and 12h of exposure. Our results provide a comprehensive sequence resource for studying the potato-EO interactions, revealing induced alternations in potato starch metabolism and suggesting modified regulatory mechanism in potato affected by EO.
PB  - European Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5593
ER  - 

@conference{
author = "Stupar, Sofija and Avramović, Miloš and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2019",
abstract = "Potato fields pest protection is still predominantly based on synthetic pesticides, expensive in terms of monetary investment and proved to be harmful to human health and the environment. Alternatively, flower strips of French marigold (Tagetes patula L.) appearing as potent pest repellents in potato fields, offer well-adopted practice in organic farming. Up to now, the effect of French marigoldon potato metabolism, physiology, and yield has not been studied in detail. Thus, a microarray transcriptome analysis was performed to study the effect of French marigold essential oil (EO) on laboratory-grown potato, with the focus on starch metabolism pathway. After 8h of exposure to EO, 3840 potato probes were differentially expressed (fold change ≥2) compared to control plants, of which 1744 were upregulated and 2096 downregulated. We selected a set of putative genes involved in the starch metabolism pathway in plastids, and validated microarray patterns using RT-qPCR method. The expression of selected genes was analyzed in plants exposed to EO for 4, 8 and 12h . While the expression of genes involved in starch biosynthesis was at similar levels as in non-treated plants, the most of the genes responsible for gradual degradation of starch displayed significantly elevated expression with the highest levels detected after 8 and 12h of exposure. Our results provide a comprehensive sequence resource for studying the potato-EO interactions, revealing induced alternations in potato starch metabolism and suggesting modified regulatory mechanism in potato affected by EO.",
publisher = "European Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5593"
}

Stupar, S., Avramović, M., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2019). Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
European Federation of Biotechnology., 129.
https://hdl.handle.net/21.15107/rcub_ibiss_5593

Stupar S, Avramović M, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:129.
https://hdl.handle.net/21.15107/rcub_ibiss_5593 .

Stupar, Sofija, Avramović, Miloš, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):129,
https://hdl.handle.net/21.15107/rcub_ibiss_5593 .

TY  - CONF
AU  - Devrnja, Nina
AU  - Ćosić, Tatjana
AU  - Stupar, Sofija
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Šajkunić, Sanja
AU  - Ćirić, Ana
AU  - Savić, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5594
AB  - Methanol and aqueous extracts of French marigold flowers and leaves, with previously determined chemical profiles, were subjected to in vitro antioxidant and antimicrobial assays. The methanol extract of flowers had the highest total phenolic content (TPC) according to the Folin–Ciocalteu method, and UHPLC–LTQ/orbitrap/MS analysis identified the flavonoid aglycones as the dominant class of phenolics in the extract. Likewise, the methanol extract of flowers showed the highest antioxidant activity measured by DPPH and ABTS assays. Generally, the higher TPC correlated with lower IC50 value and stronger antioxidant potential in all extracts. Microdilution test was used for determination of the minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations of French marigold extracts on following microfungi - Aspergillus versicolor, A. niger, Penicillium ochrochloron, P. verrucosum var. cyclopium and Alternaria alternata. The antimicrobial test revealed that P. ochrochloron was the most sensitive while A. niger was the most resistant microfungus to all analyzed extracts. Correspondingly, the methanol extract of flowers was shown to be the most potent one, regarding the antimicrobial activity. Additionally, antimicrobial potential of methanol extract of flowers was tested for in vivo potato tuber protection against A. alternata causing the early blight disease. Based on obtained antioxidant and antimicrobial potential, French marigold methanol extracts could provide an alternative strategy for the controlling expansion of potato pathogens.
PB  - European Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts
SP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5594
ER  - 

@conference{
author = "Devrnja, Nina and Ćosić, Tatjana and Stupar, Sofija and Gašić, Uroš and Tešić, Živoslav and Šajkunić, Sanja and Ćirić, Ana and Savić, Jelena",
year = "2019",
abstract = "Methanol and aqueous extracts of French marigold flowers and leaves, with previously determined chemical profiles, were subjected to in vitro antioxidant and antimicrobial assays. The methanol extract of flowers had the highest total phenolic content (TPC) according to the Folin–Ciocalteu method, and UHPLC–LTQ/orbitrap/MS analysis identified the flavonoid aglycones as the dominant class of phenolics in the extract. Likewise, the methanol extract of flowers showed the highest antioxidant activity measured by DPPH and ABTS assays. Generally, the higher TPC correlated with lower IC50 value and stronger antioxidant potential in all extracts. Microdilution test was used for determination of the minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations of French marigold extracts on following microfungi - Aspergillus versicolor, A. niger, Penicillium ochrochloron, P. verrucosum var. cyclopium and Alternaria alternata. The antimicrobial test revealed that P. ochrochloron was the most sensitive while A. niger was the most resistant microfungus to all analyzed extracts. Correspondingly, the methanol extract of flowers was shown to be the most potent one, regarding the antimicrobial activity. Additionally, antimicrobial potential of methanol extract of flowers was tested for in vivo potato tuber protection against A. alternata causing the early blight disease. Based on obtained antioxidant and antimicrobial potential, French marigold methanol extracts could provide an alternative strategy for the controlling expansion of potato pathogens.",
publisher = "European Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5594"
}

Devrnja, N., Ćosić, T., Stupar, S., Gašić, U., Tešić, Ž., Šajkunić, S., Ćirić, A.,& Savić, J.. (2019). Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
European Federation of Biotechnology., 130.
https://hdl.handle.net/21.15107/rcub_ibiss_5594

Devrnja N, Ćosić T, Stupar S, Gašić U, Tešić Ž, Šajkunić S, Ćirić A, Savić J. Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:130.
https://hdl.handle.net/21.15107/rcub_ibiss_5594 .

Devrnja, Nina, Ćosić, Tatjana, Stupar, Sofija, Gašić, Uroš, Tešić, Živoslav, Šajkunić, Sanja, Ćirić, Ana, Savić, Jelena, "Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):130,
https://hdl.handle.net/21.15107/rcub_ibiss_5594 .

TY  - JOUR
AU  - Savić, Jelena
AU  - Nikolić, Radomirka
AU  - Banjac, Nevena
AU  - Zdravković-Korać, Snežana
AU  - Stupar, Sofija
AU  - Cingel, Aleksandar
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161719301786?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3490
AB  - Food demands of increasing human population dictate intensification of livestock production, however, environmental stresses could jeopardize producers' efforts. Forage legumes suffer from yield losses and poor nutritional status due to salinity increase of agricultural soils. As tools aimed to reduce negative impacts of biotic or abiotic stresses, proteinase inhibitors (PIs) have been promoted for biotechnological improvements. In order to increase tolerance of Lotus corniculatus L. to salt stress, serine PI, BvSTI, was introduced into this legume using Agrobacterium rhizogenes, with final transformation efficiency of 4.57%. PCR, DNA gel-blot, RT-PCR and in-gel protein activity assays confirmed the presence and activity of BvSTI products in transformed lines. Plants from three selected transgenic lines (21, 73 and 109) showed significant alterations in overall phenotypic appearance, corresponding to differences in BvSTI accumulation. Lines 73 and 109 showed up to 7.3-fold higher number of tillers and massive, up to 5.8-fold heavier roots than in nontransformed controls (NTC). Line 21 was phenotypically similar to NTC, accumulated less BvSTI transcripts and did not exhibit an additional band of recombinant trypsin inhibitor as seen in lines 73 and 109. Exposure of the transgenic lines to NaCl revealed different levels of salt stress susceptibility. The NaCl sensitivity index, based on morphological appearance and chlorophyll concentrations showed that lines 73 and 109 were significantly less affected by salinity than NTC or line 21. High level of BvSTI altered morphology and delayed salt stress related senescence, implicating BvSTI gene as a promising tool for salinity tolerance improvement trials in L. corniculatus.
T2  - Journal of Plant Physiology
T1  - Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.
VL  - 243
DO  - 10.1016/j.jplph.2019.153055
SP  - 153055
ER  - 

@article{
author = "Savić, Jelena and Nikolić, Radomirka and Banjac, Nevena and Zdravković-Korać, Snežana and Stupar, Sofija and Cingel, Aleksandar and Ćosić, Tatjana and Raspor, Martin and Smigocki, Ann and Ninković, Slavica",
year = "2019",
abstract = "Food demands of increasing human population dictate intensification of livestock production, however, environmental stresses could jeopardize producers' efforts. Forage legumes suffer from yield losses and poor nutritional status due to salinity increase of agricultural soils. As tools aimed to reduce negative impacts of biotic or abiotic stresses, proteinase inhibitors (PIs) have been promoted for biotechnological improvements. In order to increase tolerance of Lotus corniculatus L. to salt stress, serine PI, BvSTI, was introduced into this legume using Agrobacterium rhizogenes, with final transformation efficiency of 4.57%. PCR, DNA gel-blot, RT-PCR and in-gel protein activity assays confirmed the presence and activity of BvSTI products in transformed lines. Plants from three selected transgenic lines (21, 73 and 109) showed significant alterations in overall phenotypic appearance, corresponding to differences in BvSTI accumulation. Lines 73 and 109 showed up to 7.3-fold higher number of tillers and massive, up to 5.8-fold heavier roots than in nontransformed controls (NTC). Line 21 was phenotypically similar to NTC, accumulated less BvSTI transcripts and did not exhibit an additional band of recombinant trypsin inhibitor as seen in lines 73 and 109. Exposure of the transgenic lines to NaCl revealed different levels of salt stress susceptibility. The NaCl sensitivity index, based on morphological appearance and chlorophyll concentrations showed that lines 73 and 109 were significantly less affected by salinity than NTC or line 21. High level of BvSTI altered morphology and delayed salt stress related senescence, implicating BvSTI gene as a promising tool for salinity tolerance improvement trials in L. corniculatus.",
journal = "Journal of Plant Physiology",
title = "Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.",
volume = "243",
doi = "10.1016/j.jplph.2019.153055",
pages = "153055"
}

Savić, J., Nikolić, R., Banjac, N., Zdravković-Korać, S., Stupar, S., Cingel, A., Ćosić, T., Raspor, M., Smigocki, A.,& Ninković, S.. (2019). Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.. in Journal of Plant Physiology, 243, 153055.
https://doi.org/10.1016/j.jplph.2019.153055

Savić J, Nikolić R, Banjac N, Zdravković-Korać S, Stupar S, Cingel A, Ćosić T, Raspor M, Smigocki A, Ninković S. Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.. in Journal of Plant Physiology. 2019;243:153055.
doi:10.1016/j.jplph.2019.153055 .

Savić, Jelena, Nikolić, Radomirka, Banjac, Nevena, Zdravković-Korać, Snežana, Stupar, Sofija, Cingel, Aleksandar, Ćosić, Tatjana, Raspor, Martin, Smigocki, Ann, Ninković, Slavica, "Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L." in Journal of Plant Physiology, 243 (2019):153055,
https://doi.org/10.1016/j.jplph.2019.153055 . .

TY  - CONF
AU  - Stupar, Sofija
AU  - Tešević, Vele
AU  - Stanković, Jovana
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4329
AB  - When attacked, plants activate defense mechanisms including volatile emission. Besides having
a pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.
French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground parts
of plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysis
revealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.
The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)
as the most abundant compounds. In order to investigate the potential of marigold EO to induce
the defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesis
related PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EO
volatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,
and plants were then additionally injured by mechanically wounding leaves across the leaf surface.
After 24 h of wounding, another two leaves from each plant were collected and expression
of the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,
while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression of
all genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plants
exposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar to
that detected in untreated controls. These findings support the efforts for environmentally-safe
pest control in crops by using natural compounds.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil
SP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4329
ER  - 

@conference{
author = "Stupar, Sofija and Tešević, Vele and Stanković, Jovana and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2018",
abstract = "When attacked, plants activate defense mechanisms including volatile emission. Besides having
a pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.
French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground parts
of plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysis
revealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.
The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)
as the most abundant compounds. In order to investigate the potential of marigold EO to induce
the defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesis
related PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EO
volatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,
and plants were then additionally injured by mechanically wounding leaves across the leaf surface.
After 24 h of wounding, another two leaves from each plant were collected and expression
of the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,
while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression of
all genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plants
exposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar to
that detected in untreated controls. These findings support the efforts for environmentally-safe
pest control in crops by using natural compounds.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil",
pages = "58",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4329"
}

Stupar, S., Tešević, V., Stanković, J., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2018). Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 58.
https://hdl.handle.net/21.15107/rcub_ibiss_4329

Stupar S, Tešević V, Stanković J, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;:58.
https://hdl.handle.net/21.15107/rcub_ibiss_4329 .

Stupar, Sofija, Tešević, Vele, Stanković, Jovana, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018):58,
https://hdl.handle.net/21.15107/rcub_ibiss_4329 .

TY  - CONF
AU  - Gašić, Uroš
AU  - Stupar, Sofija
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Ćosić, Tatjana
AU  - Ninković, Slavica
AU  - Tešić, Živoslav
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4328
AB  - Aqueous, ethanol and methanol extracts of aerial parts of ornamental French marigold (Tagetes
patula L.), which consist mostly of flavonoids, have been reported to exhibit different biological
activities, including cytotoxic effect on various human cancer cell lines. However, some of the
most potent flavonoids characteristic for the genus Tagetes, such as quercetagetin and patuletin,
are rare in nature. Thus, optimization of extraction process together with employment of sensitive
techniques and detection equipment should be of interest in gaining information about marigold
biologically active compounds. In the present study, separate extracts of leaves and flowers were
obtained from dry plant material by overnight incubation in methanol, dichloromethane or hot
(60 °C) water, followed by ultrasonic bath maceration. UHPLC–LTQ/orbitrap/MS, employed for separation
and identification, revealed the presence of 68 different compounds in total, belonging to
phenolic acids (20 compounds), flavonoid glycosides (37 compounds), and flavonoid aglycones
(11 compounds). The highest number of compounds was detected in methanol extracts, i.e. 55 in
flowers and 60 in leaves, while after the extraction in dichloromethane only 23 compounds were
detected in leaves and 24 in flowers. In both leaves and flowers, the most diversified were flavonoid
glycosides with quercetin and patuletin in 3, 7 or 3,7 di-O- derivativeforms. Aglycon patuletin
was detected in all analyzed extracts, while quercetagetin was detected only in methanol and
dichloromethane extracts of flowers. Obtained data confirmed T. patula extracts as a rich source of
compounds with biological and potential pharmacological values.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4328
ER  - 

@conference{
author = "Gašić, Uroš and Stupar, Sofija and Savić, Jelena and Devrnja, Nina and Ćosić, Tatjana and Ninković, Slavica and Tešić, Živoslav",
year = "2018",
abstract = "Aqueous, ethanol and methanol extracts of aerial parts of ornamental French marigold (Tagetes
patula L.), which consist mostly of flavonoids, have been reported to exhibit different biological
activities, including cytotoxic effect on various human cancer cell lines. However, some of the
most potent flavonoids characteristic for the genus Tagetes, such as quercetagetin and patuletin,
are rare in nature. Thus, optimization of extraction process together with employment of sensitive
techniques and detection equipment should be of interest in gaining information about marigold
biologically active compounds. In the present study, separate extracts of leaves and flowers were
obtained from dry plant material by overnight incubation in methanol, dichloromethane or hot
(60 °C) water, followed by ultrasonic bath maceration. UHPLC–LTQ/orbitrap/MS, employed for separation
and identification, revealed the presence of 68 different compounds in total, belonging to
phenolic acids (20 compounds), flavonoid glycosides (37 compounds), and flavonoid aglycones
(11 compounds). The highest number of compounds was detected in methanol extracts, i.e. 55 in
flowers and 60 in leaves, while after the extraction in dichloromethane only 23 compounds were
detected in leaves and 24 in flowers. In both leaves and flowers, the most diversified were flavonoid
glycosides with quercetin and patuletin in 3, 7 or 3,7 di-O- derivativeforms. Aglycon patuletin
was detected in all analyzed extracts, while quercetagetin was detected only in methanol and
dichloromethane extracts of flowers. Obtained data confirmed T. patula extracts as a rich source of
compounds with biological and potential pharmacological values.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4328"
}

Gašić, U., Stupar, S., Savić, J., Devrnja, N., Ćosić, T., Ninković, S.,& Tešić, Ž.. (2018). UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 124.
https://hdl.handle.net/21.15107/rcub_ibiss_4328

Gašić U, Stupar S, Savić J, Devrnja N, Ćosić T, Ninković S, Tešić Ž. UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;:124.
https://hdl.handle.net/21.15107/rcub_ibiss_4328 .

Gašić, Uroš, Stupar, Sofija, Savić, Jelena, Devrnja, Nina, Ćosić, Tatjana, Ninković, Slavica, Tešić, Živoslav, "UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018):124,
https://hdl.handle.net/21.15107/rcub_ibiss_4328 .