TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Motyka, Vaclav
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5856
AB  - Kohlrabi (Brassica oleracea var. gongylodes) is a vegetable cultivated for its edible stem tuber. Although valued for its nutritional properties and tolerance to abiotic stress, kohlrabi is one of the least studied brassicas. In this review, we summarize the results of our decade-long research on in vitro morphogenesis of kohlrabi, starting from 2013. Protocols for efficient in vitro regeneration with minimal requirements for external application of plant growth regulators (PGRs) have been developed, both by somatic embryogenesis (SE) and by de novo shoot organogenesis (DNSO). Efficient regeneration by direct SE was achieved using immature zygotic embryos as explants incubated on PGR-free media, and the production process was maintained in culture thanks to highly efficient secondary SE. Conversely, efficient regeneration by indirect DNSO was achieved using entire seedlings as explants and adding only cytokinin (CK) without the need for exogenous auxin. Comprehensive phytohormone analyses revealed that different exogenously applied CKs differentially affected the composition of endogenous phytohormones and induced changes in the expression of cell cycle-related genes and other genes involved in the organogenic response. The addition of high sucrose concentrations to the nutrient media failed to induce the formation of stem tubers in in vitro culture, but revealed a complex interaction with exogenously applied CKs, interfering with both the endogenous phytohormonome and the expression of organogenesis-related genes. Our work has provided substantial biotechnological advances in the field of in vitro regeneration of kohlrabi, as well as in understanding the underlying phytohormonal regulation. The review aims to introduce kohlrabi to the scientific community as a model system for both basic and applied research, while we continue seeking answers to the outstanding questions and trying to pave the way for the development of more resistant varieties.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research
IS  - 6
VL  - 9
DO  - 10.3390/horticulturae9060674
SP  - 674
ER  - 

@article{
author = "Ćosić, Tatjana and Raspor, Martin and Motyka, Vaclav and Cingel, Aleksandar and Ninković, Slavica",
year = "2023",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) is a vegetable cultivated for its edible stem tuber. Although valued for its nutritional properties and tolerance to abiotic stress, kohlrabi is one of the least studied brassicas. In this review, we summarize the results of our decade-long research on in vitro morphogenesis of kohlrabi, starting from 2013. Protocols for efficient in vitro regeneration with minimal requirements for external application of plant growth regulators (PGRs) have been developed, both by somatic embryogenesis (SE) and by de novo shoot organogenesis (DNSO). Efficient regeneration by direct SE was achieved using immature zygotic embryos as explants incubated on PGR-free media, and the production process was maintained in culture thanks to highly efficient secondary SE. Conversely, efficient regeneration by indirect DNSO was achieved using entire seedlings as explants and adding only cytokinin (CK) without the need for exogenous auxin. Comprehensive phytohormone analyses revealed that different exogenously applied CKs differentially affected the composition of endogenous phytohormones and induced changes in the expression of cell cycle-related genes and other genes involved in the organogenic response. The addition of high sucrose concentrations to the nutrient media failed to induce the formation of stem tubers in in vitro culture, but revealed a complex interaction with exogenously applied CKs, interfering with both the endogenous phytohormonome and the expression of organogenesis-related genes. Our work has provided substantial biotechnological advances in the field of in vitro regeneration of kohlrabi, as well as in understanding the underlying phytohormonal regulation. The review aims to introduce kohlrabi to the scientific community as a model system for both basic and applied research, while we continue seeking answers to the outstanding questions and trying to pave the way for the development of more resistant varieties.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research",
number = "6",
volume = "9",
doi = "10.3390/horticulturae9060674",
pages = "674"
}

Ćosić, T., Raspor, M., Motyka, V., Cingel, A.,& Ninković, S.. (2023). In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research. in Horticulturae
Basel: MDPI., 9(6), 674.
https://doi.org/10.3390/horticulturae9060674

Ćosić T, Raspor M, Motyka V, Cingel A, Ninković S. In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research. in Horticulturae. 2023;9(6):674.
doi:10.3390/horticulturae9060674 .

Ćosić, Tatjana, Raspor, Martin, Motyka, Vaclav, Cingel, Aleksandar, Ninković, Slavica, "In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research" in Horticulturae, 9, no. 6 (2023):674,
https://doi.org/10.3390/horticulturae9060674 . .

TY  - CONF
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Jevremović, Slađana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6689
AB  - Boja cveta je jedna od najznačajnijih osobina biljaka čija je osnovna uloga u privlačenju oprašivača. Takođe, boja cvetova određuje i komercijalnu vrednost ukrasnih biljaka. Ova osobina biljaka predstavlja jedan od prvih predmeta istraživanja u oblasti primene genetičkog inženjerstva u hortikulturi. Boja cvetova je određena sadržim tri grupe biljnih pigmenata: anticijanina, karotenoida i betalaina. Veliki napredak u istraživanjima je napravljen na modifikaciji boje cvetova genetičkom modifikacijom biosinteze antocijanina. Sadržaj karotenoida u cvetovima (likopen, lutein, β-karoten, anteraksantin, violaksantin) određuje boju cvetova biljaka od žute do crvene. U radu će biti prikazani rezultati na promeni boje cvetova sitnocvetne ljubičice (Viola cornuta L.) kultivara sa žutim cvetovima genetičkom modifikacijom karotenoida. Genetičkom transformacijom, posredstvom Agrobacterium tumefaciens, genom za kapsantin-kapsorubin sintazu poreklom iz cvetova ljiljana (Llccs) pomoću dva vektora (35S-Llccs i PchsA-Llccs) dobijene su biljke sa promenjenom bojom cvetova. Krunični listići cvetova biljaka su promenjeni iz žute u nijanse narandžaste usled akumulacije novosintetisanog pigmenta kapsantina. Pigment se akumulira u hromoplastima ćelija kruničnih listova usled čega je došlo do promene boje cvetova. Na osnovu ovih rezultata zaključeno je da ektopijska ekspresija gena Llccs pod uticajem oba promotora se može koristi za promenu boje cvetova biljaka.
AB  - Боја цветова је једна од најзначајнијих особина биљака чија је основна улога у привлачењу опрашивача. Такође, боја цветова одређује и комерцијалну вредност украсних биљака. Ова особина биљака представља један од првих предмета истраживања у области примене генетичког инжењерства у хортикултури. Боја цветова је одређена садржајем три групе биљних пигмената: антицијанина, каротеноида и беталаина. Велики напредак у истраживањима је направљен на модификацији боје цветова генетичком модификацијом биосинтезе антоцијанина. Садржај каротеноида у цветовима (ликопен, лутеин,  β-каротен, антераксантин, виолаксантин) одређује боју цветова биљака од жуте до црвене. У раду ће бити приказани резултати на промени боје цветова ситноцветне љубичице (Viola cornuta L.) култивара са жутим цветовима генетичком модификацијом каротеноида. Генетичком трансформацијом, посредством Agrobacterium tumefaciens, геном за капсантин-капсорубин синтазу пореклом из цветова љиљана (Llccs) помоћу два вектора (35S-Llccs и PchsA-Llccs) добијене су биљке са промењеном бојом цветова. Крунични листићи цветова биљака су промењени из жуте у нијансе наранџасте услед акумулације новосинтетисаног пигмента капсантина. Пигмент се акумулира у хромопластима ћелија круничних листића услед чега је дошло до промене боје цветова. На основу ових резултата закључено је да ектопијска експресија гена Llccs под утицајем оба промотора се може користити за промену боје цветова биљака.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.)
T1  - Najnovija postignuća na promeni boje cvetova sitnocvetne ljubičice (Viola cornutaL.)
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6689
ER  - 

@conference{
author = "Trajković, Milena and Cingel, Aleksandar and Jevremović, Slađana",
year = "2022",
abstract = "Boja cveta je jedna od najznačajnijih osobina biljaka čija je osnovna uloga u privlačenju oprašivača. Takođe, boja cvetova određuje i komercijalnu vrednost ukrasnih biljaka. Ova osobina biljaka predstavlja jedan od prvih predmeta istraživanja u oblasti primene genetičkog inženjerstva u hortikulturi. Boja cvetova je određena sadržim tri grupe biljnih pigmenata: anticijanina, karotenoida i betalaina. Veliki napredak u istraživanjima je napravljen na modifikaciji boje cvetova genetičkom modifikacijom biosinteze antocijanina. Sadržaj karotenoida u cvetovima (likopen, lutein, β-karoten, anteraksantin, violaksantin) određuje boju cvetova biljaka od žute do crvene. U radu će biti prikazani rezultati na promeni boje cvetova sitnocvetne ljubičice (Viola cornuta L.) kultivara sa žutim cvetovima genetičkom modifikacijom karotenoida. Genetičkom transformacijom, posredstvom Agrobacterium tumefaciens, genom za kapsantin-kapsorubin sintazu poreklom iz cvetova ljiljana (Llccs) pomoću dva vektora (35S-Llccs i PchsA-Llccs) dobijene su biljke sa promenjenom bojom cvetova. Krunični listići cvetova biljaka su promenjeni iz žute u nijanse narandžaste usled akumulacije novosintetisanog pigmenta kapsantina. Pigment se akumulira u hromoplastima ćelija kruničnih listova usled čega je došlo do promene boje cvetova. Na osnovu ovih rezultata zaključeno je da ektopijska ekspresija gena Llccs pod uticajem oba promotora se može koristi za promenu boje cvetova biljaka., Боја цветова је једна од најзначајнијих особина биљака чија је основна улога у привлачењу опрашивача. Такође, боја цветова одређује и комерцијалну вредност украсних биљака. Ова особина биљака представља један од првих предмета истраживања у области примене генетичког инжењерства у хортикултури. Боја цветова је одређена садржајем три групе биљних пигмената: антицијанина, каротеноида и беталаина. Велики напредак у истраживањима је направљен на модификацији боје цветова генетичком модификацијом биосинтезе антоцијанина. Садржај каротеноида у цветовима (ликопен, лутеин,  β-каротен, антераксантин, виолаксантин) одређује боју цветова биљака од жуте до црвене. У раду ће бити приказани резултати на промени боје цветова ситноцветне љубичице (Viola cornuta L.) култивара са жутим цветовима генетичком модификацијом каротеноида. Генетичком трансформацијом, посредством Agrobacterium tumefaciens, геном за капсантин-капсорубин синтазу пореклом из цветова љиљана (Llccs) помоћу два вектора (35S-Llccs и PchsA-Llccs) добијене су биљке са промењеном бојом цветова. Крунични листићи цветова биљака су промењени из жуте у нијансе наранџасте услед акумулације новосинтетисаног пигмента капсантина. Пигмент се акумулира у хромопластима ћелија круничних листића услед чега је дошло до промене боје цветова. На основу ових резултата закључено је да ектопијска експресија гена Llccs под утицајем оба промотора се може користити за промену боје цветова биљака.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.), Najnovija postignuća na promeni boje cvetova sitnocvetne ljubičice (Viola cornutaL.)",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6689"
}

Trajković, M., Cingel, A.,& Jevremović, S.. (2022). Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 70.
https://hdl.handle.net/21.15107/rcub_ibiss_6689

Trajković M, Cingel A, Jevremović S. Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:70.
https://hdl.handle.net/21.15107/rcub_ibiss_6689 .

Trajković, Milena, Cingel, Aleksandar, Jevremović, Slađana, "Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):70,
https://hdl.handle.net/21.15107/rcub_ibiss_6689 .

TY  - CHAP
AU  - Trajković, Milena
AU  - Jevremović, Slađana
AU  - Cingel, Aleksandar
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5302
AB  - U hortikulturi je prisutna stalna potreba za ukrasnim biljkama sa novim karakteristikama, gde boja
cveta predstavlja jednu od najvažnijih osobina koja određuje njihovu komercijalnu vrednost. Sa razvojem
metoda genetičkog inženjeringa otvorena je mogućnost kreiranja biljaka sa željenom bojom cvetova koja
se ne može postići klasičnim ukrštanjem ili mutagenezom. Boja cvetova kod biljaka određena je sadržajem
tri biljna pigmenta: antocijanina, karotenoida i betalaina. Do sada, najveći napredak postignut je genetičkom
modulacijom biosinteze antocijanina. Na ovaj način postignute su nove boje cvetova kod
najmanje 50 ukrasnih vrsta, a neki od tih modifikovanih varijeteta su već dugi niz godina u slobodnoj prodaji.
Međutim, promena boje cveta manipulacijom biosintetskog puta karotenoida je dokumentovana
kod svega nekoliko ukrasnih vrsta i poslednjih godina intenzivirana su istraživanja u tom pravcu. U ovom
radu je razmatran potencijal ovog pristupa, sa posebnim osvrtom na rezultate postignute na promeni boje
cvetova kod kultivara ljubičice uvodjenjem gena za kapsantin-kapsorubin sintazu.
AB  - In horticulture, there is a constant need for ornamental plants with new characteristics, where the flower
color is one of the most important features that determines their commercial value. With the development
of genetic engineering methods, it has been possible to create plants with the desired flower
color which cannot be achieved by classical breeding or mutagenesis. The flower color in plants is determined
by the content of three plant pigments: anthocyanins, carotenoids and betalains. Up to date, the
greatest progress has been made by genetic modulation of anthocyanin biosynthesis. In this way, the new
flower colors have been achieved in at least 50 ornamental species, and some of these modified varieties
have been on market for many years. However, the alteration of flower color by manipulating the carotenoid
biosynthetic pathway has been documented in only a few ornamental species, and the research has
been significantly increased last few years. In this paper, the potential of this approach is considered, with
special reference to the results achieved on flower color alteration of pansy cultivars by introducing the
gene for capsanthin-capsorubin synthase.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering
T2  - Trends in Molecular Biology
T1  - Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida
T1  - Recent advances in flower color alteration by metabolic manipulation of carotenoid biosynthesis
VL  - 2
SP  - 238
EP  - 253
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5302
ER  - 

@inbook{
author = "Trajković, Milena and Jevremović, Slađana and Cingel, Aleksandar",
year = "2022",
abstract = "U hortikulturi je prisutna stalna potreba za ukrasnim biljkama sa novim karakteristikama, gde boja
cveta predstavlja jednu od najvažnijih osobina koja određuje njihovu komercijalnu vrednost. Sa razvojem
metoda genetičkog inženjeringa otvorena je mogućnost kreiranja biljaka sa željenom bojom cvetova koja
se ne može postići klasičnim ukrštanjem ili mutagenezom. Boja cvetova kod biljaka određena je sadržajem
tri biljna pigmenta: antocijanina, karotenoida i betalaina. Do sada, najveći napredak postignut je genetičkom
modulacijom biosinteze antocijanina. Na ovaj način postignute su nove boje cvetova kod
najmanje 50 ukrasnih vrsta, a neki od tih modifikovanih varijeteta su već dugi niz godina u slobodnoj prodaji.
Međutim, promena boje cveta manipulacijom biosintetskog puta karotenoida je dokumentovana
kod svega nekoliko ukrasnih vrsta i poslednjih godina intenzivirana su istraživanja u tom pravcu. U ovom
radu je razmatran potencijal ovog pristupa, sa posebnim osvrtom na rezultate postignute na promeni boje
cvetova kod kultivara ljubičice uvodjenjem gena za kapsantin-kapsorubin sintazu., In horticulture, there is a constant need for ornamental plants with new characteristics, where the flower
color is one of the most important features that determines their commercial value. With the development
of genetic engineering methods, it has been possible to create plants with the desired flower
color which cannot be achieved by classical breeding or mutagenesis. The flower color in plants is determined
by the content of three plant pigments: anthocyanins, carotenoids and betalains. Up to date, the
greatest progress has been made by genetic modulation of anthocyanin biosynthesis. In this way, the new
flower colors have been achieved in at least 50 ornamental species, and some of these modified varieties
have been on market for many years. However, the alteration of flower color by manipulating the carotenoid
biosynthetic pathway has been documented in only a few ornamental species, and the research has
been significantly increased last few years. In this paper, the potential of this approach is considered, with
special reference to the results achieved on flower color alteration of pansy cultivars by introducing the
gene for capsanthin-capsorubin synthase.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering",
journal = "Trends in Molecular Biology",
booktitle = "Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida, Recent advances in flower color alteration by metabolic manipulation of carotenoid biosynthesis",
volume = "2",
pages = "238-253",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5302"
}

Trajković, M., Jevremović, S.,& Cingel, A.. (2022). Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida. in Trends in Molecular Biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering., 2, 238-253.
https://hdl.handle.net/21.15107/rcub_ibiss_5302

Trajković M, Jevremović S, Cingel A. Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida. in Trends in Molecular Biology. 2022;2:238-253.
https://hdl.handle.net/21.15107/rcub_ibiss_5302 .

Trajković, Milena, Jevremović, Slađana, Cingel, Aleksandar, "Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida" in Trends in Molecular Biology, 2 (2022):238-253,
https://hdl.handle.net/21.15107/rcub_ibiss_5302 .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Gašić, Uroš
AU  - Šajkunić, Sanja
AU  - Cingel, Aleksandar
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/3/407
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4789
AB  - French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antiox-idant activity statistically comparable with positive control Trolox, was chosen for testing of anti-feedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.
PB  - Basel: MDPI
T2  - Plants
T1  - UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle
IS  - 3
VL  - 11
DO  - 10.3390/plants11030407
SP  - 407
ER  - 

@article{
author = "Devrnja, Nina and Gašić, Uroš and Šajkunić, Sanja and Cingel, Aleksandar and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antiox-idant activity statistically comparable with positive control Trolox, was chosen for testing of anti-feedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle",
number = "3",
volume = "11",
doi = "10.3390/plants11030407",
pages = "407"
}

Devrnja, N., Gašić, U., Šajkunić, S., Cingel, A., Stupar, S., Tubić, L.,& Savić, J.. (2022). UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle. in Plants
Basel: MDPI., 11(3), 407.
https://doi.org/10.3390/plants11030407

Devrnja N, Gašić U, Šajkunić S, Cingel A, Stupar S, Tubić L, Savić J. UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle. in Plants. 2022;11(3):407.
doi:10.3390/plants11030407 .

Devrnja, Nina, Gašić, Uroš, Šajkunić, Sanja, Cingel, Aleksandar, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, "UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle" in Plants, 11, no. 3 (2022):407,
https://doi.org/10.3390/plants11030407 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Milošević, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4119
AB  - Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.
PB  - Basel, Switzerland: MDPI
T2  - Plants
T1  - Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery
IS  - 1
VL  - 10
DO  - 10.3390/plants10010154
SP  - 154
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Dragićević, Milan and Simonović, Ana and Milošević, Snežana",
year = "2021",
abstract = "Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.",
publisher = "Basel, Switzerland: MDPI",
journal = "Plants",
title = "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery",
number = "1",
volume = "10",
doi = "10.3390/plants10010154",
pages = "154"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M., Cingel, A., Dragićević, M., Simonović, A.,& Milošević, S.. (2021). Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants
Basel, Switzerland: MDPI., 10(1), 154.
https://doi.org/10.3390/plants10010154

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Cingel A, Dragićević M, Simonović A, Milošević S. Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants. 2021;10(1):154.
doi:10.3390/plants10010154 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Dragićević, Milan, Simonović, Ana, Milošević, Snežana, "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery" in Plants, 10, no. 1 (2021):154,
https://doi.org/10.3390/plants10010154 . .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Stanković-Jeremić, Jovana
AU  - Maksimović, Vuk
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - internal-pdf://Stupar et al. - 2021 - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf St.pdf
UR  - https://www.mdpi.com/2223-7747/10/1/172
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4147
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI AG
T2  - Plants
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
SP  - 172
ER  - 

@article{
author = "Stupar, Sofija and Dragićević, Milan and Tešević, Vele and Stanković-Jeremić, Jovana and Maksimović, Vuk and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI AG",
journal = "Plants",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172",
pages = "172"
}

Stupar, S., Dragićević, M., Tešević, V., Stanković-Jeremić, J., Maksimović, V., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants
MDPI AG., 10(1), 172.
https://doi.org/10.3390/plants10010172

Stupar S, Dragićević M, Tešević V, Stanković-Jeremić J, Maksimović V, Ćosić T, Devrnja N, Tubić L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants. 2021;10(1):172.
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragićević, Milan, Tešević, Vele, Stanković-Jeremić, Jovana, Maksimović, Vuk, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants, 10, no. 1 (2021):172,
https://doi.org/10.3390/plants10010172 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Kaleri, Abdul Rasheed
AU  - Ninković, Slavica
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ćosić, Tatjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4303
AB  - De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs
IS  - 16
VL  - 22
DO  - 10.3390/ijms22168554
SP  - 8554
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Kaleri, Abdul Rasheed and Ninković, Slavica and Tubić, Ljiljana and Cingel, Aleksandar and Ćosić, Tatjana",
year = "2021",
abstract = "De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs",
number = "16",
volume = "22",
doi = "10.3390/ijms22168554",
pages = "8554"
}

Raspor, M., Motyka, V., Kaleri, A. R., Ninković, S., Tubić, L., Cingel, A.,& Ćosić, T.. (2021). Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences
Basel: MDPI., 22(16), 8554.
https://doi.org/10.3390/ijms22168554

Raspor M, Motyka V, Kaleri AR, Ninković S, Tubić L, Cingel A, Ćosić T. Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences. 2021;22(16):8554.
doi:10.3390/ijms22168554 .

Raspor, Martin, Motyka, Václav, Kaleri, Abdul Rasheed, Ninković, Slavica, Tubić, Ljiljana, Cingel, Aleksandar, Ćosić, Tatjana, "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs" in International Journal of Molecular Sciences, 22, no. 16 (2021):8554,
https://doi.org/10.3390/ijms22168554 . .

TY  - JOUR
AU  - Trajković, Milena
AU  - Jevremović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Subotić, Angelina
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4434
AB  - Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis
IS  - 9
VL  - 7
DO  - 10.3390/horticulturae7090324
SP  - 324
ER  - 

@article{
author = "Trajković, Milena and Jevremović, Slađana and Dragićević, Milan and Simonović, Ana and Subotić, Angelina and Milošević, Snežana and Cingel, Aleksandar",
year = "2021",
abstract = "Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis",
number = "9",
volume = "7",
doi = "10.3390/horticulturae7090324",
pages = "324"
}

Trajković, M., Jevremović, S., Dragićević, M., Simonović, A., Subotić, A., Milošević, S.,& Cingel, A.. (2021). Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis. in Horticulturae
Basel: MDPI., 7(9), 324.
https://doi.org/10.3390/horticulturae7090324

Trajković M, Jevremović S, Dragićević M, Simonović A, Subotić A, Milošević S, Cingel A. Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis. in Horticulturae. 2021;7(9):324.
doi:10.3390/horticulturae7090324 .

Trajković, Milena, Jevremović, Slađana, Dragićević, Milan, Simonović, Ana, Subotić, Angelina, Milošević, Snežana, Cingel, Aleksandar, "Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis" in Horticulturae, 7, no. 9 (2021):324,
https://doi.org/10.3390/horticulturae7090324 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - CHAP
AU  - Raspor, Martin
AU  - Cingel, Aleksandar
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4302
AB  - Significant limitations in potato production are crop loss due to the damage
made by insect pests, and the cost of enormous amount of chemicals, harmful
to humans and environment, extensively used in their control. As an alternative,
development of genetically modified potato offered possibility for pest management
in a more sustainable, environmentally friendly way. Over the past 30 years
introduction of pest resistance traits progressed from a single gene to multiple
stacked events and from Bt-toxin expression to expression of proteins from non-Bt
sources, dsRNA and their combination, while advances in molecular biology have
brought “cleaner” gene manipulation technologies. However, together with benefits
any new technology also bears its risks, and there are still a range of unanswered
questions and concerns about long-term impact of genetically modified crops –
that with knowledge and precautionary approaches can be avoided or mitigated.
Sustainability of genetically modified crops for pest control largely depends on the
willingness to gain and implement such knowledge.
PB  - IntechOpen
T2  - Solanum tuberosum - a Promising Crop for Starvation Problem
T1  - Genetically Modified Potato for Pest Resistance: Thrift or Threat?
DO  - 10.5772/intechopen.98748
ER  - 

@inbook{
editor = "Yildiz, Mustafa, Ozgen, Yasin",
author = "Raspor, Martin and Cingel, Aleksandar",
year = "2021",
abstract = "Significant limitations in potato production are crop loss due to the damage
made by insect pests, and the cost of enormous amount of chemicals, harmful
to humans and environment, extensively used in their control. As an alternative,
development of genetically modified potato offered possibility for pest management
in a more sustainable, environmentally friendly way. Over the past 30 years
introduction of pest resistance traits progressed from a single gene to multiple
stacked events and from Bt-toxin expression to expression of proteins from non-Bt
sources, dsRNA and their combination, while advances in molecular biology have
brought “cleaner” gene manipulation technologies. However, together with benefits
any new technology also bears its risks, and there are still a range of unanswered
questions and concerns about long-term impact of genetically modified crops –
that with knowledge and precautionary approaches can be avoided or mitigated.
Sustainability of genetically modified crops for pest control largely depends on the
willingness to gain and implement such knowledge.",
publisher = "IntechOpen",
journal = "Solanum tuberosum - a Promising Crop for Starvation Problem",
booktitle = "Genetically Modified Potato for Pest Resistance: Thrift or Threat?",
doi = "10.5772/intechopen.98748"
}

Yildiz, M., Ozgen, Y., Raspor, M.,& Cingel, A.. (2021). Genetically Modified Potato for Pest Resistance: Thrift or Threat?. in Solanum tuberosum - a Promising Crop for Starvation Problem
IntechOpen..
https://doi.org/10.5772/intechopen.98748

Yildiz M, Ozgen Y, Raspor M, Cingel A. Genetically Modified Potato for Pest Resistance: Thrift or Threat?. in Solanum tuberosum - a Promising Crop for Starvation Problem. 2021;.
doi:10.5772/intechopen.98748 .

Yildiz, Mustafa, Ozgen, Yasin, Raspor, Martin, Cingel, Aleksandar, "Genetically Modified Potato for Pest Resistance: Thrift or Threat?" in Solanum tuberosum - a Promising Crop for Starvation Problem (2021),
https://doi.org/10.5772/intechopen.98748 . .

TY  - CONF
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Vujičić, Milorad
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Milošević, Snežana
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4898
AB  - Impatiens walleriana (Balsaminaceae) is a worldwide popular horticultural plant and commercially produced in Serbia for many years. Plants have high requirements for the presence of water in the substrate, which deficiency leads to a rapid drop in the cells turgor pressure and tissue dehydration. This is the main problem in commercial production of these plants, especially during transport and sale process when plants are not always sufficiently supplied with water. We assessed the drought and recovery effect on growth, physiological and biochemical parameters in I. walleriana (Xtreme Scarlet) grown in chamber under controlled physical conditions (ex vitro). Plants were 74 days old when the drought stress was imposed. We examined: control plants – grown under optimal watering (37%-40% of soil moisture content), drought stressed plants – (25%, 15%, 10% and 5% of soil moisture content), and recovery plants – rehydrated for four days to reach optimum soil moisture content (37%-40%). Drought reduces fresh (FW) and dry weight (DW) of shoots, as well as total leaf area and shoots water potential. Drought significantly increased abscisic acid (ABA) content in the leaves in order to reduce transpiration rate and preserve water in it᾽s tissue. The concentration of hydrogen peroxide (H2O2) and malondialdehyde (MDA) were increased in drought-stressed plants, as a consequence of drought induced oxidative stress in the plant cells. The activity of antioxidant enzymes (superoxide dismutase - SOD, peroxidase - POX and catalase - CAT) contributed to neutralizing negative effects of oxidative stress on plant growth, while the different SOD and POX isoforms were detected on gel. Recovery treatment neutralised negative effect of drought on growth, physiological and biochemical parameters analysed in I. walleriana to a certain extent.
PB  - Brussels: European Plant Science Organisation (EPSO)
C3  - Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy
T1  - Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro
SP  - 240
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4898
ER  - 

@conference{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Vujičić, Milorad and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Milošević, Snežana",
year = "2021",
abstract = "Impatiens walleriana (Balsaminaceae) is a worldwide popular horticultural plant and commercially produced in Serbia for many years. Plants have high requirements for the presence of water in the substrate, which deficiency leads to a rapid drop in the cells turgor pressure and tissue dehydration. This is the main problem in commercial production of these plants, especially during transport and sale process when plants are not always sufficiently supplied with water. We assessed the drought and recovery effect on growth, physiological and biochemical parameters in I. walleriana (Xtreme Scarlet) grown in chamber under controlled physical conditions (ex vitro). Plants were 74 days old when the drought stress was imposed. We examined: control plants – grown under optimal watering (37%-40% of soil moisture content), drought stressed plants – (25%, 15%, 10% and 5% of soil moisture content), and recovery plants – rehydrated for four days to reach optimum soil moisture content (37%-40%). Drought reduces fresh (FW) and dry weight (DW) of shoots, as well as total leaf area and shoots water potential. Drought significantly increased abscisic acid (ABA) content in the leaves in order to reduce transpiration rate and preserve water in it᾽s tissue. The concentration of hydrogen peroxide (H2O2) and malondialdehyde (MDA) were increased in drought-stressed plants, as a consequence of drought induced oxidative stress in the plant cells. The activity of antioxidant enzymes (superoxide dismutase - SOD, peroxidase - POX and catalase - CAT) contributed to neutralizing negative effects of oxidative stress on plant growth, while the different SOD and POX isoforms were detected on gel. Recovery treatment neutralised negative effect of drought on growth, physiological and biochemical parameters analysed in I. walleriana to a certain extent.",
publisher = "Brussels: European Plant Science Organisation (EPSO)",
journal = "Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy",
title = "Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro",
pages = "240",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4898"
}

Đurić, M., Subotić, A., Prokić, L., Vujičić, M., Trifunović-Momčilov, M., Cingel, A.,& Milošević, S.. (2021). Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro. in Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy
Brussels: European Plant Science Organisation (EPSO)., 240.
https://hdl.handle.net/21.15107/rcub_ibiss_4898

Đurić M, Subotić A, Prokić L, Vujičić M, Trifunović-Momčilov M, Cingel A, Milošević S. Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro. in Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy. 2021;:240.
https://hdl.handle.net/21.15107/rcub_ibiss_4898 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Vujičić, Milorad, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Milošević, Snežana, "Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro" in Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy (2021):240,
https://hdl.handle.net/21.15107/rcub_ibiss_4898 .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Cingel, Aleksandar
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1449
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3996
AB  - Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors-ancymidol (A) and paclobutrazol (P)-the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb's dormancy and its sprouting.
PB  - MDPI AG
T2  - Plants (Basel, Switzerland)
T1  - Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.
IS  - 11
VL  - 9
DO  - 10.3390/plants9111449
SP  - 1449
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Cingel, Aleksandar and Milošević, Snežana and Jevremović, Slađana and Subotić, Angelina",
year = "2020",
abstract = "Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors-ancymidol (A) and paclobutrazol (P)-the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb's dormancy and its sprouting.",
publisher = "MDPI AG",
journal = "Plants (Basel, Switzerland)",
title = "Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.",
number = "11",
volume = "9",
doi = "10.3390/plants9111449",
pages = "1449"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Cingel, A., Milošević, S., Jevremović, S.,& Subotić, A.. (2020). Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.. in Plants (Basel, Switzerland)
MDPI AG., 9(11), 1449.
https://doi.org/10.3390/plants9111449

Marković M, Trifunović-Momčilov M, Uzelac B, Cingel A, Milošević S, Jevremović S, Subotić A. Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.. in Plants (Basel, Switzerland). 2020;9(11):1449.
doi:10.3390/plants9111449 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Cingel, Aleksandar, Milošević, Snežana, Jevremović, Slađana, Subotić, Angelina, "Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content." in Plants (Basel, Switzerland), 9, no. 11 (2020):1449,
https://doi.org/10.3390/plants9111449 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Vujičić, Milorad
AU  - Milošević, Snežana
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1559
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4008
AB  - This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.
PB  - MDPI AG
T2  - Plants
T1  - Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro
IS  - 11
VL  - 9
DO  - 10.3390/plants9111559
SP  - 1559
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Vujičić, Milorad and Milošević, Snežana",
year = "2020",
abstract = "This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.",
publisher = "MDPI AG",
journal = "Plants",
title = "Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro",
number = "11",
volume = "9",
doi = "10.3390/plants9111559",
pages = "1559"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M., Cingel, A., Vujičić, M.,& Milošević, S.. (2020). Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro. in Plants
MDPI AG., 9(11), 1559.
https://doi.org/10.3390/plants9111559

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Cingel A, Vujičić M, Milošević S. Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro. in Plants. 2020;9(11):1559.
doi:10.3390/plants9111559 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Vujičić, Milorad, Milošević, Snežana, "Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro" in Plants, 9, no. 11 (2020):1559,
https://doi.org/10.3390/plants9111559 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Cingel, Aleksandar
AU  - Ghalawnji, Nabil
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3816
AB  - Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of
the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators
(PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were
studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18±2°C on half-strength
MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose
and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA),
gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all
treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a
distinctive manner, with a variety of morphological traits being altered in comparison to matching
controls.
PB  - Serbian Biological Socitey
T2  - Archives of Biological Sciences
T1  - Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro
IS  - 3
VL  - 72
DO  - 10.2298/ABS200622029C
SP  - 349
EP  - 357
ER  - 

@article{
author = "Ćosić, Tatjana and Savić, Jelena and Raspor, Martin and Cingel, Aleksandar and Ghalawnji, Nabil and Vinterhalter, Branka and Ninković, Slavica",
year = "2020",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of
the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators
(PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were
studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18±2°C on half-strength
MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose
and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA),
gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all
treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a
distinctive manner, with a variety of morphological traits being altered in comparison to matching
controls.",
publisher = "Serbian Biological Socitey",
journal = "Archives of Biological Sciences",
title = "Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro",
number = "3",
volume = "72",
doi = "10.2298/ABS200622029C",
pages = "349-357"
}

Ćosić, T., Savić, J., Raspor, M., Cingel, A., Ghalawnji, N., Vinterhalter, B.,& Ninković, S.. (2020). Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. in Archives of Biological Sciences
Serbian Biological Socitey., 72(3), 349-357.
https://doi.org/10.2298/ABS200622029C

Ćosić T, Savić J, Raspor M, Cingel A, Ghalawnji N, Vinterhalter B, Ninković S. Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. in Archives of Biological Sciences. 2020;72(3):349-357.
doi:10.2298/ABS200622029C .

Ćosić, Tatjana, Savić, Jelena, Raspor, Martin, Cingel, Aleksandar, Ghalawnji, Nabil, Vinterhalter, Branka, Ninković, Slavica, "Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro" in Archives of Biological Sciences, 72, no. 3 (2020):349-357,
https://doi.org/10.2298/ABS200622029C . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Dobrev, Petre I.
AU  - Malbeck, Jiří
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2020
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7044434
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3623
AB  - A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.
T2  - Scientific Reports
T1  - Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-60412-9
SP  - 3437
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Dobrev, Petre I. and Malbeck, Jiří and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Tadić, Vojin and Dragićević, Ivana",
year = "2020",
abstract = "A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.",
journal = "Scientific Reports",
title = "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-60412-9",
pages = "3437"
}

Raspor, M., Motyka, V., Ninković, S., Dobrev, P. I., Malbeck, J., Ćosić, T., Cingel, A., Savić, J., Tadić, V.,& Dragićević, I.. (2020). Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports, 10(1), 3437.
https://doi.org/10.1038/s41598-020-60412-9

Raspor M, Motyka V, Ninković S, Dobrev PI, Malbeck J, Ćosić T, Cingel A, Savić J, Tadić V, Dragićević I. Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports. 2020;10(1):3437.
doi:10.1038/s41598-020-60412-9 .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Dobrev, Petre I., Malbeck, Jiří, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Tadić, Vojin, Dragićević, Ivana, "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics." in Scientific Reports, 10, no. 1 (2020):3437,
https://doi.org/10.1038/s41598-020-60412-9 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Matekalo, Dragana
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161718304140?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3213
AB  - Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species.
T2  - Journal of Plant Physiology
T1  - Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.
VL  - 232
DO  - 10.1016/j.jplph.2018.11.004
SP  - 257
EP  - 269
ER  - 

@article{
author = "Ćosić, Tatjana and Raspor, Martin and Savić, Jelena and Cingel, Aleksandar and Matekalo, Dragana and Zdravković-Korać, Snežana and Ninković, Slavica",
year = "2019",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species.",
journal = "Journal of Plant Physiology",
title = "Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.",
volume = "232",
doi = "10.1016/j.jplph.2018.11.004",
pages = "257-269"
}

Ćosić, T., Raspor, M., Savić, J., Cingel, A., Matekalo, D., Zdravković-Korać, S.,& Ninković, S.. (2019). Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.. in Journal of Plant Physiology, 232, 257-269.
https://doi.org/10.1016/j.jplph.2018.11.004

Ćosić T, Raspor M, Savić J, Cingel A, Matekalo D, Zdravković-Korać S, Ninković S. Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.. in Journal of Plant Physiology. 2019;232:257-269.
doi:10.1016/j.jplph.2018.11.004 .

Ćosić, Tatjana, Raspor, Martin, Savić, Jelena, Cingel, Aleksandar, Matekalo, Dragana, Zdravković-Korać, Snežana, Ninković, Slavica, "Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi." in Journal of Plant Physiology, 232 (2019):257-269,
https://doi.org/10.1016/j.jplph.2018.11.004 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6129
AB  - Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.
PB  - Olomouc: Europian Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration
SP  - 131
EP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6129
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Vaclav and Savić, Jelena and Cingel, Aleksandar and Raspor, Martin and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2019",
abstract = "Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.",
publisher = "Olomouc: Europian Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration",
pages = "131-131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6129"
}

Ćosić, T., Motyka, V., Savić, J., Cingel, A., Raspor, M., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2019). Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
Olomouc: Europian Federation of Biotechnology., 131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129

Ćosić T, Motyka V, Savić J, Cingel A, Raspor M, Devrnja N, Dobrev PI, Ninković S. Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

Ćosić, Tatjana, Motyka, Vaclav, Savić, Jelena, Cingel, Aleksandar, Raspor, Martin, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):131-131,
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

TY  - CONF
AU  - Stupar, Sofija
AU  - Avramović, Miloš
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5593
AB  - Potato fields pest protection is still predominantly based on synthetic pesticides, expensive in terms of monetary investment and proved to be harmful to human health and the environment. Alternatively, flower strips of French marigold (Tagetes patula L.) appearing as potent pest repellents in potato fields, offer well-adopted practice in organic farming. Up to now, the effect of French marigoldon potato metabolism, physiology, and yield has not been studied in detail. Thus, a microarray transcriptome analysis was performed to study the effect of French marigold essential oil (EO) on laboratory-grown potato, with the focus on starch metabolism pathway. After 8h of exposure to EO, 3840 potato probes were differentially expressed (fold change ≥2) compared to control plants, of which 1744 were upregulated and 2096 downregulated. We selected a set of putative genes involved in the starch metabolism pathway in plastids, and validated microarray patterns using RT-qPCR method. The expression of selected genes was analyzed in plants exposed to EO for 4, 8 and 12h . While the expression of genes involved in starch biosynthesis was at similar levels as in non-treated plants, the most of the genes responsible for gradual degradation of starch displayed significantly elevated expression with the highest levels detected after 8 and 12h of exposure. Our results provide a comprehensive sequence resource for studying the potato-EO interactions, revealing induced alternations in potato starch metabolism and suggesting modified regulatory mechanism in potato affected by EO.
PB  - European Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5593
ER  - 

@conference{
author = "Stupar, Sofija and Avramović, Miloš and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2019",
abstract = "Potato fields pest protection is still predominantly based on synthetic pesticides, expensive in terms of monetary investment and proved to be harmful to human health and the environment. Alternatively, flower strips of French marigold (Tagetes patula L.) appearing as potent pest repellents in potato fields, offer well-adopted practice in organic farming. Up to now, the effect of French marigoldon potato metabolism, physiology, and yield has not been studied in detail. Thus, a microarray transcriptome analysis was performed to study the effect of French marigold essential oil (EO) on laboratory-grown potato, with the focus on starch metabolism pathway. After 8h of exposure to EO, 3840 potato probes were differentially expressed (fold change ≥2) compared to control plants, of which 1744 were upregulated and 2096 downregulated. We selected a set of putative genes involved in the starch metabolism pathway in plastids, and validated microarray patterns using RT-qPCR method. The expression of selected genes was analyzed in plants exposed to EO for 4, 8 and 12h . While the expression of genes involved in starch biosynthesis was at similar levels as in non-treated plants, the most of the genes responsible for gradual degradation of starch displayed significantly elevated expression with the highest levels detected after 8 and 12h of exposure. Our results provide a comprehensive sequence resource for studying the potato-EO interactions, revealing induced alternations in potato starch metabolism and suggesting modified regulatory mechanism in potato affected by EO.",
publisher = "European Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5593"
}

Stupar, S., Avramović, M., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2019). Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
European Federation of Biotechnology., 129.
https://hdl.handle.net/21.15107/rcub_ibiss_5593

Stupar S, Avramović M, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:129.
https://hdl.handle.net/21.15107/rcub_ibiss_5593 .

Stupar, Sofija, Avramović, Miloš, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):129,
https://hdl.handle.net/21.15107/rcub_ibiss_5593 .

TY  - JOUR
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Cingel, Aleksandar
AU  - Ghalawenji, Nabil
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2019
UR  - http://link.springer.com/10.1007/s13205-018-1540-4
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6314937
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3232
AB  - The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.
T2  - 3 Biotech
T1  - Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.
IS  - 1
VL  - 9
DO  - 10.1007/s13205-018-1540-4
SP  - 17
ER  - 

@article{
author = "Trajković, Milena and Antonić Reljin, Dragana and Cingel, Aleksandar and Ghalawenji, Nabil and Subotić, Angelina and Jevremović, Slađana",
year = "2019",
abstract = "The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.",
journal = "3 Biotech",
title = "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.",
number = "1",
volume = "9",
doi = "10.1007/s13205-018-1540-4",
pages = "17"
}

Trajković, M., Antonić Reljin, D., Cingel, A., Ghalawenji, N., Subotić, A.,& Jevremović, S.. (2019). Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech, 9(1), 17.
https://doi.org/10.1007/s13205-018-1540-4

Trajković M, Antonić Reljin D, Cingel A, Ghalawenji N, Subotić A, Jevremović S. Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech. 2019;9(1):17.
doi:10.1007/s13205-018-1540-4 .

Trajković, Milena, Antonić Reljin, Dragana, Cingel, Aleksandar, Ghalawenji, Nabil, Subotić, Angelina, Jevremović, Slađana, "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta." in 3 Biotech, 9, no. 1 (2019):17,
https://doi.org/10.1007/s13205-018-1540-4 . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Nikolić, Radomirka
AU  - Banjac, Nevena
AU  - Zdravković-Korać, Snežana
AU  - Stupar, Sofija
AU  - Cingel, Aleksandar
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161719301786?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3490
AB  - Food demands of increasing human population dictate intensification of livestock production, however, environmental stresses could jeopardize producers' efforts. Forage legumes suffer from yield losses and poor nutritional status due to salinity increase of agricultural soils. As tools aimed to reduce negative impacts of biotic or abiotic stresses, proteinase inhibitors (PIs) have been promoted for biotechnological improvements. In order to increase tolerance of Lotus corniculatus L. to salt stress, serine PI, BvSTI, was introduced into this legume using Agrobacterium rhizogenes, with final transformation efficiency of 4.57%. PCR, DNA gel-blot, RT-PCR and in-gel protein activity assays confirmed the presence and activity of BvSTI products in transformed lines. Plants from three selected transgenic lines (21, 73 and 109) showed significant alterations in overall phenotypic appearance, corresponding to differences in BvSTI accumulation. Lines 73 and 109 showed up to 7.3-fold higher number of tillers and massive, up to 5.8-fold heavier roots than in nontransformed controls (NTC). Line 21 was phenotypically similar to NTC, accumulated less BvSTI transcripts and did not exhibit an additional band of recombinant trypsin inhibitor as seen in lines 73 and 109. Exposure of the transgenic lines to NaCl revealed different levels of salt stress susceptibility. The NaCl sensitivity index, based on morphological appearance and chlorophyll concentrations showed that lines 73 and 109 were significantly less affected by salinity than NTC or line 21. High level of BvSTI altered morphology and delayed salt stress related senescence, implicating BvSTI gene as a promising tool for salinity tolerance improvement trials in L. corniculatus.
T2  - Journal of Plant Physiology
T1  - Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.
VL  - 243
DO  - 10.1016/j.jplph.2019.153055
SP  - 153055
ER  - 

@article{
author = "Savić, Jelena and Nikolić, Radomirka and Banjac, Nevena and Zdravković-Korać, Snežana and Stupar, Sofija and Cingel, Aleksandar and Ćosić, Tatjana and Raspor, Martin and Smigocki, Ann and Ninković, Slavica",
year = "2019",
abstract = "Food demands of increasing human population dictate intensification of livestock production, however, environmental stresses could jeopardize producers' efforts. Forage legumes suffer from yield losses and poor nutritional status due to salinity increase of agricultural soils. As tools aimed to reduce negative impacts of biotic or abiotic stresses, proteinase inhibitors (PIs) have been promoted for biotechnological improvements. In order to increase tolerance of Lotus corniculatus L. to salt stress, serine PI, BvSTI, was introduced into this legume using Agrobacterium rhizogenes, with final transformation efficiency of 4.57%. PCR, DNA gel-blot, RT-PCR and in-gel protein activity assays confirmed the presence and activity of BvSTI products in transformed lines. Plants from three selected transgenic lines (21, 73 and 109) showed significant alterations in overall phenotypic appearance, corresponding to differences in BvSTI accumulation. Lines 73 and 109 showed up to 7.3-fold higher number of tillers and massive, up to 5.8-fold heavier roots than in nontransformed controls (NTC). Line 21 was phenotypically similar to NTC, accumulated less BvSTI transcripts and did not exhibit an additional band of recombinant trypsin inhibitor as seen in lines 73 and 109. Exposure of the transgenic lines to NaCl revealed different levels of salt stress susceptibility. The NaCl sensitivity index, based on morphological appearance and chlorophyll concentrations showed that lines 73 and 109 were significantly less affected by salinity than NTC or line 21. High level of BvSTI altered morphology and delayed salt stress related senescence, implicating BvSTI gene as a promising tool for salinity tolerance improvement trials in L. corniculatus.",
journal = "Journal of Plant Physiology",
title = "Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.",
volume = "243",
doi = "10.1016/j.jplph.2019.153055",
pages = "153055"
}

Savić, J., Nikolić, R., Banjac, N., Zdravković-Korać, S., Stupar, S., Cingel, A., Ćosić, T., Raspor, M., Smigocki, A.,& Ninković, S.. (2019). Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.. in Journal of Plant Physiology, 243, 153055.
https://doi.org/10.1016/j.jplph.2019.153055

Savić J, Nikolić R, Banjac N, Zdravković-Korać S, Stupar S, Cingel A, Ćosić T, Raspor M, Smigocki A, Ninković S. Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.. in Journal of Plant Physiology. 2019;243:153055.
doi:10.1016/j.jplph.2019.153055 .

Savić, Jelena, Nikolić, Radomirka, Banjac, Nevena, Zdravković-Korać, Snežana, Stupar, Sofija, Cingel, Aleksandar, Ćosić, Tatjana, Raspor, Martin, Smigocki, Ann, Ninković, Slavica, "Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L." in Journal of Plant Physiology, 243 (2019):153055,
https://doi.org/10.1016/j.jplph.2019.153055 . .

TY  - CONF
AU  - Trajković, Milena
AU  - Jeknić, Zoran
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Cingel, Aleksandar
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6540
AB  - Viola cornuta L. ‘Lutea Splendens’ is a perennial ornamental plant with small yellow flowers that
naturally grows in the Pyrenees in Spain and France. To develop novel cultivars with orange and
red flower colors, we performed Agrobacterium tumefaciens (LBA4404)-mediated transformation
with the binary vector pWBVec10a/P35S::Llccs::TNos that harbored capsanthin-capsorubin synthase
(Llccs) gene from Lilium lancifolium under the control of CaMV35S constitutive promoter and
the nopaline synthase (Nos) terminator. Capsanthin-capsorubin synthase catalyzes the conversion
of anteraxanthin and violaxanthin, two yellow ubiquitous 5-6-epoxy-xanthophylls, into capsanthin
and capsorubin, two red xanthophylls, respectively. Starting with hypocotyl explants, we developed
a transformation protocol with 0.3% shoot regeneration efficiency. Histochemical assay for
β-glucuronidase (GUS) activity showed uidA reporter gene expression in all putative Llccs-transgenic
shoots. The presence of Llccs transgene, hygromycin phosphotransferase (hpt) selectable
marker gene and uidA (GUS) reporter gene in all putative Llccs-transgenic lines were confirmed
by PCR analysis. This is the first report on Agrobacterium-mediated genetic transformation of V.
cornuta L. with the aim to introduce desirable traits into this species.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6540
ER  - 

@conference{
author = "Trajković, Milena and Jeknić, Zoran and Antonić Reljin, Dragana and Subotić, Angelina and Jevremović, Slađana and Cingel, Aleksandar",
year = "2018",
abstract = "Viola cornuta L. ‘Lutea Splendens’ is a perennial ornamental plant with small yellow flowers that
naturally grows in the Pyrenees in Spain and France. To develop novel cultivars with orange and
red flower colors, we performed Agrobacterium tumefaciens (LBA4404)-mediated transformation
with the binary vector pWBVec10a/P35S::Llccs::TNos that harbored capsanthin-capsorubin synthase
(Llccs) gene from Lilium lancifolium under the control of CaMV35S constitutive promoter and
the nopaline synthase (Nos) terminator. Capsanthin-capsorubin synthase catalyzes the conversion
of anteraxanthin and violaxanthin, two yellow ubiquitous 5-6-epoxy-xanthophylls, into capsanthin
and capsorubin, two red xanthophylls, respectively. Starting with hypocotyl explants, we developed
a transformation protocol with 0.3% shoot regeneration efficiency. Histochemical assay for
β-glucuronidase (GUS) activity showed uidA reporter gene expression in all putative Llccs-transgenic
shoots. The presence of Llccs transgene, hygromycin phosphotransferase (hpt) selectable
marker gene and uidA (GUS) reporter gene in all putative Llccs-transgenic lines were confirmed
by PCR analysis. This is the first report on Agrobacterium-mediated genetic transformation of V.
cornuta L. with the aim to introduce desirable traits into this species.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene",
pages = "152",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6540"
}

Trajković, M., Jeknić, Z., Antonić Reljin, D., Subotić, A., Jevremović, S.,& Cingel, A.. (2018). Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade., 152.
https://hdl.handle.net/21.15107/rcub_ibiss_6540

Trajković M, Jeknić Z, Antonić Reljin D, Subotić A, Jevremović S, Cingel A. Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:152.
https://hdl.handle.net/21.15107/rcub_ibiss_6540 .

Trajković, Milena, Jeknić, Zoran, Antonić Reljin, Dragana, Subotić, Angelina, Jevremović, Slađana, Cingel, Aleksandar, "Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):152,
https://hdl.handle.net/21.15107/rcub_ibiss_6540 .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4318
AB  - Fluctuations in local phytohormone levels are considered to be important for initiation of developmental events in the process of plant organogenesis. Simultaneously, sugars as signaling molecules affect plant metabolism and growth, potentially interacting with hormonal regulation. Thus, the aim of presented study was to see whether there were any changes in cytokinin (CK) homeostasis in 4 different stages (T1-T4) during de novo shoot organogenesis of kohlrabi (Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings under the influence of exogenous CKs, trans-zeatin (transZ) and thidiazuron (TDZ), combined with high sucrose concentration. Explants grown on CK-free medium with 3% sucrose were used as control. Generally, analysis of variance showed statistically significant impact of CK treatment and sucrose concentration, as well as their interaction in all investigated stages, including seedling development before calli formation (T1 and T2), calli formation (T3) and when de novo shoots occurred (T4). The obtained CK profiles exposed remarkable increase in total CK levels in samples treated with transZ, particularly when 9% sucrose was used in nutrition medium, reaching up to 50 000 pmol/g FW. This trend was observed for all CK physiological and structural groups. On the other hand, application of a synthetic urea-type CK, TDZ, contributed to little or no increase in the endogenous CK levels regardless the sucrose concentration. Our results demonstrated that phytohormones metabolism may be triggered by sucrose signaling in kohlrabi shoot organogenesis.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts
T1  - Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4318
ER  - 

@conference{
editor = "Uzelac, Branka",
author = "Ćosić, Tatjana and Motyka, Vaclav and Savić, Jelena and Cingel, Aleksandar and Raspor, Martin and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2018",
abstract = "Fluctuations in local phytohormone levels are considered to be important for initiation of developmental events in the process of plant organogenesis. Simultaneously, sugars as signaling molecules affect plant metabolism and growth, potentially interacting with hormonal regulation. Thus, the aim of presented study was to see whether there were any changes in cytokinin (CK) homeostasis in 4 different stages (T1-T4) during de novo shoot organogenesis of kohlrabi (Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings under the influence of exogenous CKs, trans-zeatin (transZ) and thidiazuron (TDZ), combined with high sucrose concentration. Explants grown on CK-free medium with 3% sucrose were used as control. Generally, analysis of variance showed statistically significant impact of CK treatment and sucrose concentration, as well as their interaction in all investigated stages, including seedling development before calli formation (T1 and T2), calli formation (T3) and when de novo shoots occurred (T4). The obtained CK profiles exposed remarkable increase in total CK levels in samples treated with transZ, particularly when 9% sucrose was used in nutrition medium, reaching up to 50 000 pmol/g FW. This trend was observed for all CK physiological and structural groups. On the other hand, application of a synthetic urea-type CK, TDZ, contributed to little or no increase in the endogenous CK levels regardless the sucrose concentration. Our results demonstrated that phytohormones metabolism may be triggered by sucrose signaling in kohlrabi shoot organogenesis.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts",
title = "Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4318"
}

Uzelac, B., Ćosić, T., Motyka, V., Savić, J., Cingel, A., Raspor, M., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2018). Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_4318

Uzelac B, Ćosić T, Motyka V, Savić J, Cingel A, Raspor M, Devrnja N, Dobrev PI, Ninković S. Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts. 2018;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_4318 .

Uzelac, Branka, Ćosić, Tatjana, Motyka, Vaclav, Savić, Jelena, Cingel, Aleksandar, Raspor, Martin, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts (2018):39,
https://hdl.handle.net/21.15107/rcub_ibiss_4318 .

TY  - CONF
AU  - Antonić Reljin, Dragana
AU  - Trailović, Maja
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6394
AB  - Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %).
PB  - Belgrade: Serbian Biological Socitey
C3  - Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
T1  - Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6394
ER  - 

@conference{
author = "Antonić Reljin, Dragana and Trailović, Maja and Trajković, Milena and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana",
year = "2018",
abstract = "Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %).",
publisher = "Belgrade: Serbian Biological Socitey",
journal = "Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija",
title = "Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6394"
}

Antonić Reljin, D., Trailović, M., Trajković, M., Cingel, A., Subotić, A.,& Jevremović, S.. (2018). Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
Belgrade: Serbian Biological Socitey., 60.
https://hdl.handle.net/21.15107/rcub_ibiss_6394

Antonić Reljin D, Trailović M, Trajković M, Cingel A, Subotić A, Jevremović S. Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija. 2018;:60.
https://hdl.handle.net/21.15107/rcub_ibiss_6394 .

Antonić Reljin, Dragana, Trailović, Maja, Trajković, Milena, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, "Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull" in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija (2018):60,
https://hdl.handle.net/21.15107/rcub_ibiss_6394 .

TY  - CONF
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Cingel, Aleksandar
AU  - Zdravković-Korać, Snežana
AU  - Matekalo, Dragana
AU  - Ćosić, Tatjana
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6128
AB  - In most plant species, typical protocols for de novo shoot organogenesis (DNSO) under controlled in vitro conditions include a series of two growth media; the first containing auxin for callus induction, and the second containing cytokinin (CK) which induces shoot regeneration.
However, the research conducted on kohlrabi (Brassica oleracea var. gongylodes) has shown that intact seedlings are capable of forming calli and regenerating shoots on media containing only CK, with no requirement for exogenously applied auxins. Analyses of endogenous phytohormonal status during kohlrabi DNSO showed that the presence of CKs in growth media contributed to elevated endogenous auxin (i.e. IAA) levels and IAA/bioactive CKs ratio in kohlrabi shoots. Crosstalk between cytokinins and auxins is an important factor in de novo shoot organogenesis. Thus, the present study was designed to examine whether successful DNSO, occurring in intact kohlrabi seedlings through the influence of cytokinin containing media, was accompanied by distinct alterations in the expression levels of genes implicated in auxin transport, cytokinin homeostasis regulation and de novo shoot apical meristem formation. The activity of PIN3, PIN4, ARR5, RGD3 and CUC1 genes was determined by quantitative PCR analysis. In the most cases, the expression of analysed genes had a characteristic two phase response with two declines in expression. These decreases in respect to expression in non-treated controls corresponded to time points in which formation of calli (for the first decline) and de novo shoots (for the second decline) were observed. The expression of genes demonstrated patterns that clearly correlated with CK-induced morphological manifestations of DNSO. Additional study is needed to further clarify molecular mechanisms underlying kohlrabi in vitro regeneration.
PB  - London: Springer Nature
C3  - 14th Quadrennial Congress of the IAP; 2018 Aug 19-24; Dublin, Ireland
T1  - Exogenous cytokinins affect genes involved in kohlrabi auxin transport and shoot apical meristem development
DO  - 10.1007/s11627-018-9921-2
SP  - S46
EP  - S47
ER  - 

@conference{
author = "Ninković, Slavica and Savić, Jelena and Raspor, Martin and Cingel, Aleksandar and Zdravković-Korać, Snežana and Matekalo, Dragana and Ćosić, Tatjana",
year = "2018",
abstract = "In most plant species, typical protocols for de novo shoot organogenesis (DNSO) under controlled in vitro conditions include a series of two growth media; the first containing auxin for callus induction, and the second containing cytokinin (CK) which induces shoot regeneration.
However, the research conducted on kohlrabi (Brassica oleracea var. gongylodes) has shown that intact seedlings are capable of forming calli and regenerating shoots on media containing only CK, with no requirement for exogenously applied auxins. Analyses of endogenous phytohormonal status during kohlrabi DNSO showed that the presence of CKs in growth media contributed to elevated endogenous auxin (i.e. IAA) levels and IAA/bioactive CKs ratio in kohlrabi shoots. Crosstalk between cytokinins and auxins is an important factor in de novo shoot organogenesis. Thus, the present study was designed to examine whether successful DNSO, occurring in intact kohlrabi seedlings through the influence of cytokinin containing media, was accompanied by distinct alterations in the expression levels of genes implicated in auxin transport, cytokinin homeostasis regulation and de novo shoot apical meristem formation. The activity of PIN3, PIN4, ARR5, RGD3 and CUC1 genes was determined by quantitative PCR analysis. In the most cases, the expression of analysed genes had a characteristic two phase response with two declines in expression. These decreases in respect to expression in non-treated controls corresponded to time points in which formation of calli (for the first decline) and de novo shoots (for the second decline) were observed. The expression of genes demonstrated patterns that clearly correlated with CK-induced morphological manifestations of DNSO. Additional study is needed to further clarify molecular mechanisms underlying kohlrabi in vitro regeneration.",
publisher = "London: Springer Nature",
journal = "14th Quadrennial Congress of the IAP; 2018 Aug 19-24; Dublin, Ireland",
title = "Exogenous cytokinins affect genes involved in kohlrabi auxin transport and shoot apical meristem development",
doi = "10.1007/s11627-018-9921-2",
pages = "S46-S47"
}

Ninković, S., Savić, J., Raspor, M., Cingel, A., Zdravković-Korać, S., Matekalo, D.,& Ćosić, T.. (2018). Exogenous cytokinins affect genes involved in kohlrabi auxin transport and shoot apical meristem development. in 14th Quadrennial Congress of the IAP; 2018 Aug 19-24; Dublin, Ireland
London: Springer Nature., S46-S47.
https://doi.org/10.1007/s11627-018-9921-2

Ninković S, Savić J, Raspor M, Cingel A, Zdravković-Korać S, Matekalo D, Ćosić T. Exogenous cytokinins affect genes involved in kohlrabi auxin transport and shoot apical meristem development. in 14th Quadrennial Congress of the IAP; 2018 Aug 19-24; Dublin, Ireland. 2018;:S46-S47.
doi:10.1007/s11627-018-9921-2 .

Ninković, Slavica, Savić, Jelena, Raspor, Martin, Cingel, Aleksandar, Zdravković-Korać, Snežana, Matekalo, Dragana, Ćosić, Tatjana, "Exogenous cytokinins affect genes involved in kohlrabi auxin transport and shoot apical meristem development" in 14th Quadrennial Congress of the IAP; 2018 Aug 19-24; Dublin, Ireland (2018):S46-S47,
https://doi.org/10.1007/s11627-018-9921-2 . .

TY  - CONF
AU  - Stupar, Sofija
AU  - Tešević, Vele
AU  - Stanković, Jovana
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4329
AB  - When attacked, plants activate defense mechanisms including volatile emission. Besides having
a pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.
French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground parts
of plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysis
revealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.
The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)
as the most abundant compounds. In order to investigate the potential of marigold EO to induce
the defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesis
related PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EO
volatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,
and plants were then additionally injured by mechanically wounding leaves across the leaf surface.
After 24 h of wounding, another two leaves from each plant were collected and expression
of the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,
while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression of
all genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plants
exposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar to
that detected in untreated controls. These findings support the efforts for environmentally-safe
pest control in crops by using natural compounds.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil
SP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4329
ER  - 

@conference{
author = "Stupar, Sofija and Tešević, Vele and Stanković, Jovana and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2018",
abstract = "When attacked, plants activate defense mechanisms including volatile emission. Besides having
a pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.
French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground parts
of plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysis
revealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.
The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)
as the most abundant compounds. In order to investigate the potential of marigold EO to induce
the defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesis
related PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EO
volatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,
and plants were then additionally injured by mechanically wounding leaves across the leaf surface.
After 24 h of wounding, another two leaves from each plant were collected and expression
of the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,
while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression of
all genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plants
exposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar to
that detected in untreated controls. These findings support the efforts for environmentally-safe
pest control in crops by using natural compounds.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil",
pages = "58",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4329"
}

Stupar, S., Tešević, V., Stanković, J., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2018). Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 58.
https://hdl.handle.net/21.15107/rcub_ibiss_4329

Stupar S, Tešević V, Stanković J, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;:58.
https://hdl.handle.net/21.15107/rcub_ibiss_4329 .

Stupar, Sofija, Tešević, Vele, Stanković, Jovana, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018):58,
https://hdl.handle.net/21.15107/rcub_ibiss_4329 .