TY  - JOUR
AU  - Devrnja, Nina
AU  - Anđelković, Boban
AU  - Ljujić, Jovana
AU  - Ćosić, Tatjana
AU  - Stupar, Sofija
AU  - Milutinović, Milica
AU  - Savić, Jelena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6632
AB  - β-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils
(FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation
method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and
monoterpenoids were the dominant chemical groups in total FEO, while in BEO, these two groups
occurred along with sesquiterpenes and sesquiterpenoids. The presence of dominant compounds
from both EOs was validated using the FT-IR spectra of ICs, which indicated successful complexation.
Analyses conducted using SPME/GC-MS showed the continuous emission of volatiles over 24 h from
both ICs. Under SEM, particles of both ICs appeared to have a rectangular or rhomboid morphology
and few aggregates. The insecticidal properties of EOs and ICs with β-CD were tested on the
Colorado potato beetle (CPB) as a model pest. The inclusion complex of β-CD with FEO altered the
developmental dynamic and body mass of the CPB. The initial increase in the proteolytic activity of
CPB larvae fed with potato plants sprayed with ICs was not maintained for long, and the proteolytic
efficacy of treated larvae remained in line with that of the control larvae. Future investigations will
focus on manipulating the volume of EOs used and the treatment duration for optimal efficacy and
potential application.
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation
IS  - 3
VL  - 14
DO  - 10.3390/biom14030353
SP  - 353
ER  - 

@article{
author = "Devrnja, Nina and Anđelković, Boban and Ljujić, Jovana and Ćosić, Tatjana and Stupar, Sofija and Milutinović, Milica and Savić, Jelena",
year = "2024",
abstract = "β-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils
(FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation
method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and
monoterpenoids were the dominant chemical groups in total FEO, while in BEO, these two groups
occurred along with sesquiterpenes and sesquiterpenoids. The presence of dominant compounds
from both EOs was validated using the FT-IR spectra of ICs, which indicated successful complexation.
Analyses conducted using SPME/GC-MS showed the continuous emission of volatiles over 24 h from
both ICs. Under SEM, particles of both ICs appeared to have a rectangular or rhomboid morphology
and few aggregates. The insecticidal properties of EOs and ICs with β-CD were tested on the
Colorado potato beetle (CPB) as a model pest. The inclusion complex of β-CD with FEO altered the
developmental dynamic and body mass of the CPB. The initial increase in the proteolytic activity of
CPB larvae fed with potato plants sprayed with ICs was not maintained for long, and the proteolytic
efficacy of treated larvae remained in line with that of the control larvae. Future investigations will
focus on manipulating the volume of EOs used and the treatment duration for optimal efficacy and
potential application.",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation",
number = "3",
volume = "14",
doi = "10.3390/biom14030353",
pages = "353"
}

Devrnja, N., Anđelković, B., Ljujić, J., Ćosić, T., Stupar, S., Milutinović, M.,& Savić, J.. (2024). Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation. in Biomolecules
Basel: MDPI., 14(3), 353.
https://doi.org/10.3390/biom14030353

Devrnja N, Anđelković B, Ljujić J, Ćosić T, Stupar S, Milutinović M, Savić J. Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation. in Biomolecules. 2024;14(3):353.
doi:10.3390/biom14030353 .

Devrnja, Nina, Anđelković, Boban, Ljujić, Jovana, Ćosić, Tatjana, Stupar, Sofija, Milutinović, Milica, Savić, Jelena, "Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation" in Biomolecules, 14, no. 3 (2024):353,
https://doi.org/10.3390/biom14030353 . .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6372
AB  - French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.
PB  - Springer Nature
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil
DO  - 10.1007/s00344-023-11131-8
ER  - 

@article{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2023",
abstract = "French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.",
publisher = "Springer Nature",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil",
doi = "10.1007/s00344-023-11131-8"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2023). Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation
Springer Nature..
https://doi.org/10.1007/s00344-023-11131-8

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation. 2023;.
doi:10.1007/s00344-023-11131-8 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil" in Journal of Plant Growth Regulation (2023),
https://doi.org/10.1007/s00344-023-11131-8 . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Todorović, Miloš
AU  - Gašić, Uroš
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ivković, Đurđa
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Aničić, Neda
AU  - Banjanac, Tijana
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5505
AB  - The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening
IS  - 2
VL  - 12
DO  - 10.3390/antiox12020346
SP  - 346
ER  - 

@article{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Todorović, Miloš and Gašić, Uroš and Živković, Suzana and Skorić, Marijana and Ivković, Đurđa and Savić, Jelena and Devrnja, Nina and Aničić, Neda and Banjanac, Tijana and Mojović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening",
number = "2",
volume = "12",
doi = "10.3390/antiox12020346",
pages = "346"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Todorović, M., Gašić, U., Živković, S., Skorić, M., Ivković, Đ., Savić, J., Devrnja, N., Aničić, N., Banjanac, T., Mojović, M.,& Mišić, D.. (2023). Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants
Basel: MDPI., 12(2), 346.
https://doi.org/10.3390/antiox12020346

Milutinović M, Nakarada Đ, Božunović J, Todorović M, Gašić U, Živković S, Skorić M, Ivković Đ, Savić J, Devrnja N, Aničić N, Banjanac T, Mojović M, Mišić D. Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants. 2023;12(2):346.
doi:10.3390/antiox12020346 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Todorović, Miloš, Gašić, Uroš, Živković, Suzana, Skorić, Marijana, Ivković, Đurđa, Savić, Jelena, Devrnja, Nina, Aničić, Neda, Banjanac, Tijana, Mojović, Miloš, Mišić, Danijela, "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening" in Antioxidants, 12, no. 2 (2023):346,
https://doi.org/10.3390/antiox12020346 . .

TY  - DATA
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5388
AB  - Flower strips of French marigold are commonly used pest repellents in potato fields. However, the effect of French marigold volatiles on potato metabolism is unknown. Since it is shown that different volatiles affect phytohormone signaling pathways, it is assumed that phytohormones have an important role in response to essential oils. Thus, a microarray transcriptome analysis and annotation of differentialy expresed transcripts into different phytohormone metabolic pathways was performed to study the effects of French marigold essential oil on laboratory-grown potato phytohormone homeostasis. Obtained research data are presented in 4 csv files. Names of the files are abbreviated names of corresponding phytohormone groups. Supporting information about the data are presented in the Read Me file.
T1  - Microarray data of phytohormone related genes in potato exposed to French marigold essential oil
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5388
ER  - 

@misc{
author = "Stupar, Sofija and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2023",
abstract = "Flower strips of French marigold are commonly used pest repellents in potato fields. However, the effect of French marigold volatiles on potato metabolism is unknown. Since it is shown that different volatiles affect phytohormone signaling pathways, it is assumed that phytohormones have an important role in response to essential oils. Thus, a microarray transcriptome analysis and annotation of differentialy expresed transcripts into different phytohormone metabolic pathways was performed to study the effects of French marigold essential oil on laboratory-grown potato phytohormone homeostasis. Obtained research data are presented in 4 csv files. Names of the files are abbreviated names of corresponding phytohormone groups. Supporting information about the data are presented in the Read Me file.",
title = "Microarray data of phytohormone related genes in potato exposed to French marigold essential oil",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5388"
}

Stupar, S., Devrnja, N., Tubić, L.,& Savić, J.. (2023). Microarray data of phytohormone related genes in potato exposed to French marigold essential oil. .
https://hdl.handle.net/21.15107/rcub_ibiss_5388

Stupar S, Devrnja N, Tubić L, Savić J. Microarray data of phytohormone related genes in potato exposed to French marigold essential oil. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5388 .

Stupar, Sofija, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Microarray data of phytohormone related genes in potato exposed to French marigold essential oil" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5388 .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Raspor, Martin
AU  - Sajid, Sumbal
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5364
AB  - This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5364
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Václav and Raspor, Martin and Sajid, Sumbal and Savić, Jelena and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2022",
abstract = "This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5364"
}

Ćosić, T., Motyka, V., Raspor, M., Sajid, S., Savić, J., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2022). Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364

Ćosić T, Motyka V, Raspor M, Sajid S, Savić J, Devrnja N, Dobrev PI, Ninković S. Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

Ćosić, Tatjana, Motyka, Václav, Raspor, Martin, Sajid, Sumbal, Savić, Jelena, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):24,
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

TY  - CONF
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5049
AB  - French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
T1  - Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5049
ER  - 

@conference{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022",
title = "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5049"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2022). Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
Belgrade: Serbian Plant Physiology Society., 76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022. 2022;:76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil" in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022 (2022):76,
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

TY  - JOUR
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Pěnčík, Aleš
AU  - Ćalić, Dušica
AU  - Bogdanović, Milica
AU  - Trajković, Milena
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5065
AB  - Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action
VL  - 13
DO  - 0.3389/fpls.2022.875528
SP  - 875528
ER  - 

@article{
author = "Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Pěnčík, Aleš and Ćalić, Dušica and Bogdanović, Milica and Trajković, Milena and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action",
volume = "13",
doi = "0.3389/fpls.2022.875528",
pages = "875528"
}

Smailagić, D., Banjac, N., Ninković, S., Savić, J., Ćosić, T., Pěnčík, A., Ćalić, D., Bogdanović, M., Trajković, M.,& Stanišić, M.. (2022). New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 13, 875528.
https://doi.org/0.3389/fpls.2022.875528

Smailagić D, Banjac N, Ninković S, Savić J, Ćosić T, Pěnčík A, Ćalić D, Bogdanović M, Trajković M, Stanišić M. New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science. 2022;13:875528.
doi:0.3389/fpls.2022.875528 .

Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Pěnčík, Aleš, Ćalić, Dušica, Bogdanović, Milica, Trajković, Milena, Stanišić, Mariana, "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action" in Frontiers in Plant Science, 13 (2022):875528,
https://doi.org/0.3389/fpls.2022.875528 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Milutinović, Milica
AU  - Savić, Jelena
PY  - 2022
UR  - https://www.mdpi.com/2071-1050/14/11/6847
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5008
AB  - Crop protection still mostly relies on synthetic pesticides for crop pest control. However, the rationale for their continued use is shaded by the revealed adverse effects, such as relatively long environmental persistence that leads to water and soil contamination and retention of residues in food that brings high risks to human and animal health. As part of integrated pest management, biopesticides may provide crop protection, being eco-friendly and safe for humans and non-target organisms. Essential oils, complex mixtures of low-molecular-weight, highly volatile compounds, have been highlighted as major candidates for plant-derived bioinsecticides that are up to the sustainable biological standard. In this review, we screened the insecticidal activity of essential oils or their purified compounds, with focus given to their modes of action, along with the analyzed advantages and problems associated with their wider usage as plant-derived insecticides in agriculture.
PB  - Basel: MDPI
T2  - Sustainability
T1  - When Scent Becomes a Weapon—Plant Essential Oils as Potent Bioinsecticides
IS  - 11
VL  - 14
DO  - 10.3390/su14116847
SP  - 6847
ER  - 

@article{
author = "Devrnja, Nina and Milutinović, Milica and Savić, Jelena",
year = "2022",
abstract = "Crop protection still mostly relies on synthetic pesticides for crop pest control. However, the rationale for their continued use is shaded by the revealed adverse effects, such as relatively long environmental persistence that leads to water and soil contamination and retention of residues in food that brings high risks to human and animal health. As part of integrated pest management, biopesticides may provide crop protection, being eco-friendly and safe for humans and non-target organisms. Essential oils, complex mixtures of low-molecular-weight, highly volatile compounds, have been highlighted as major candidates for plant-derived bioinsecticides that are up to the sustainable biological standard. In this review, we screened the insecticidal activity of essential oils or their purified compounds, with focus given to their modes of action, along with the analyzed advantages and problems associated with their wider usage as plant-derived insecticides in agriculture.",
publisher = "Basel: MDPI",
journal = "Sustainability",
title = "When Scent Becomes a Weapon—Plant Essential Oils as Potent Bioinsecticides",
number = "11",
volume = "14",
doi = "10.3390/su14116847",
pages = "6847"
}

Devrnja, N., Milutinović, M.,& Savić, J.. (2022). When Scent Becomes a Weapon—Plant Essential Oils as Potent Bioinsecticides. in Sustainability
Basel: MDPI., 14(11), 6847.
https://doi.org/10.3390/su14116847

Devrnja N, Milutinović M, Savić J. When Scent Becomes a Weapon—Plant Essential Oils as Potent Bioinsecticides. in Sustainability. 2022;14(11):6847.
doi:10.3390/su14116847 .

Devrnja, Nina, Milutinović, Milica, Savić, Jelena, "When Scent Becomes a Weapon—Plant Essential Oils as Potent Bioinsecticides" in Sustainability, 14, no. 11 (2022):6847,
https://doi.org/10.3390/su14116847 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Gašić, Uroš
AU  - Šajkunić, Sanja
AU  - Cingel, Aleksandar
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/3/407
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4789
AB  - French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antiox-idant activity statistically comparable with positive control Trolox, was chosen for testing of anti-feedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.
PB  - Basel: MDPI
T2  - Plants
T1  - UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle
IS  - 3
VL  - 11
DO  - 10.3390/plants11030407
SP  - 407
ER  - 

@article{
author = "Devrnja, Nina and Gašić, Uroš and Šajkunić, Sanja and Cingel, Aleksandar and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antiox-idant activity statistically comparable with positive control Trolox, was chosen for testing of anti-feedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle",
number = "3",
volume = "11",
doi = "10.3390/plants11030407",
pages = "407"
}

Devrnja, N., Gašić, U., Šajkunić, S., Cingel, A., Stupar, S., Tubić, L.,& Savić, J.. (2022). UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle. in Plants
Basel: MDPI., 11(3), 407.
https://doi.org/10.3390/plants11030407

Devrnja N, Gašić U, Šajkunić S, Cingel A, Stupar S, Tubić L, Savić J. UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle. in Plants. 2022;11(3):407.
doi:10.3390/plants11030407 .

Devrnja, Nina, Gašić, Uroš, Šajkunić, Sanja, Cingel, Aleksandar, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, "UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle" in Plants, 11, no. 3 (2022):407,
https://doi.org/10.3390/plants11030407 . .

TY  - CONF
AU  - Milutinović, Milica
AU  - Jovanović, Olivera
AU  - Devrnja, Nina
AU  - Todorović, Miloš
AU  - Živković, Suzana
AU  - Savić, Jelena
AU  - Skorić, Marijana
AU  - Puač, Nevena
AU  - Škoro, Nikola
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5269
AB  - Non-thermal temperature plasmas (NTPs) have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas phase and, in case of water treatment, in gas/liquid interface in liquid [1]. NTPs can be applied in direct treatments of plant samples or indirectly when treated water, called Plasma Activated Water (PAW), is used in treatments. In both cases RONS (short or long-living) are responsible for triggering various mechanisms and effects in plant cells. RONS have a dual role and a dose-dependent effect, they can regulate the normal physiological activities of plants as signaling molecules at the range of physiological concentration, and can trigger damage to lipids, proteins and DNA at too high or too low concentration. Plants integrate RONS with genetic, epigenetic and external signals to regulate developmental processes. RONS signaling is highly integrated with hormonal signaling networks, thereby allowing plants to adjust to environmental cues.
All organisms have adaptive responses to oxidative stress, with antioxidant enzymes (i.e. catalase, peroxidase, superoxide dismutase) being induced by changes in the levels of H2O2 or O2 •-, leading to the activation or silencing of genes encoding defensive enzymes and transcription factors [2]. Although various observations have led to the suggestion that cells have the means to sense RONS and to induce specific responses, the underlying mechanisms are still not fully understood. In addition, plants also have non-enzymatic systems known to remove RONS, which are important players in plant processes that use RONS-dependent signaling mechanisms [3]. In this work, we have shown, by using molecular approaches, how PAW treatment affect expression of genes coding for specialized metabolites and hormones, thus regulating development and stress responses in the model plant system Arabidopsis thaliana.
PB  - Bratislava: FMFI UK
C3  - Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
T1  - Molecular response to PAW in model plant species
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5269
ER  - 

@conference{
author = "Milutinović, Milica and Jovanović, Olivera and Devrnja, Nina and Todorović, Miloš and Živković, Suzana and Savić, Jelena and Skorić, Marijana and Puač, Nevena and Škoro, Nikola",
year = "2022",
abstract = "Non-thermal temperature plasmas (NTPs) have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas phase and, in case of water treatment, in gas/liquid interface in liquid [1]. NTPs can be applied in direct treatments of plant samples or indirectly when treated water, called Plasma Activated Water (PAW), is used in treatments. In both cases RONS (short or long-living) are responsible for triggering various mechanisms and effects in plant cells. RONS have a dual role and a dose-dependent effect, they can regulate the normal physiological activities of plants as signaling molecules at the range of physiological concentration, and can trigger damage to lipids, proteins and DNA at too high or too low concentration. Plants integrate RONS with genetic, epigenetic and external signals to regulate developmental processes. RONS signaling is highly integrated with hormonal signaling networks, thereby allowing plants to adjust to environmental cues.
All organisms have adaptive responses to oxidative stress, with antioxidant enzymes (i.e. catalase, peroxidase, superoxide dismutase) being induced by changes in the levels of H2O2 or O2 •-, leading to the activation or silencing of genes encoding defensive enzymes and transcription factors [2]. Although various observations have led to the suggestion that cells have the means to sense RONS and to induce specific responses, the underlying mechanisms are still not fully understood. In addition, plants also have non-enzymatic systems known to remove RONS, which are important players in plant processes that use RONS-dependent signaling mechanisms [3]. In this work, we have shown, by using molecular approaches, how PAW treatment affect expression of genes coding for specialized metabolites and hormones, thus regulating development and stress responses in the model plant system Arabidopsis thaliana.",
publisher = "Bratislava: FMFI UK",
journal = "Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia",
title = "Molecular response to PAW in model plant species",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5269"
}

Milutinović, M., Jovanović, O., Devrnja, N., Todorović, M., Živković, S., Savić, J., Skorić, M., Puač, N.,& Škoro, N.. (2022). Molecular response to PAW in model plant species. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
Bratislava: FMFI UK., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5269

Milutinović M, Jovanović O, Devrnja N, Todorović M, Živković S, Savić J, Skorić M, Puač N, Škoro N. Molecular response to PAW in model plant species. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. 2022;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5269 .

Milutinović, Milica, Jovanović, Olivera, Devrnja, Nina, Todorović, Miloš, Živković, Suzana, Savić, Jelena, Skorić, Marijana, Puač, Nevena, Škoro, Nikola, "Molecular response to PAW in model plant species" in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia (2022):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5269 .

TY  - CONF
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Živković, Suzana
AU  - Savić, Jelena
AU  - Skorić, Marijana
AU  - Devrnja, Nina
AU  - Banjanac, Tijana
AU  - Todorović, Miloš
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5270
AB  - Identifying plant species tolerant to hypoxia and determining mechanisms this tolerance is standing upon may aid in improving nutritional quality of fruits of waterlogging-intolerant crops. Berries of Solanum dulcamara L. (bittersweet), a Eurasian species belonging to the Solanaceae family that grows in both dry habitats and wetlands, represent a suitable hypoxia-tolerant model species to study redox processes in relation to fruit development. Using biochemical and physicochemical analyses, we examined differences in enzymatic and non-enzymatic components of the antioxidant system of S. dulcamara during the transition from mature green (MG) to ripe red (RR) developmental stage. By combining untargeted (UHPLC/Orbitrap MSn) and targeted (UHPLC-DAD MS2) metabolomics approaches, we revealed a significant change in polyphenolic profile of S. dulcamara fruits during fruit ripening. In order to improve knowledge on the link between metabolism and cell redox status, Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) of reactive oxygen species (ROS) in intact fruits, fruit sections and extracts, were performed in parallel with the analysis of tissue-specific distribution of antioxidants and activity of antioxidant enzymes, which demonstrated alterations in the redox state and the increase of total antioxidant capacity of bittersweet berries during ripening, especially of outer layers (pericarp) of the fruits. This knowledge could provide important information to direct research on fruit quality improvement of flood-intolerant crops that are a close relatives of bittersweet, such as tomato and eggplant.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations
SP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5270
ER  - 

@conference{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Gašić, Uroš and Živković, Suzana and Savić, Jelena and Skorić, Marijana and Devrnja, Nina and Banjanac, Tijana and Todorović, Miloš and Mojović, Miloš and Mišić, Danijela",
year = "2022",
abstract = "Identifying plant species tolerant to hypoxia and determining mechanisms this tolerance is standing upon may aid in improving nutritional quality of fruits of waterlogging-intolerant crops. Berries of Solanum dulcamara L. (bittersweet), a Eurasian species belonging to the Solanaceae family that grows in both dry habitats and wetlands, represent a suitable hypoxia-tolerant model species to study redox processes in relation to fruit development. Using biochemical and physicochemical analyses, we examined differences in enzymatic and non-enzymatic components of the antioxidant system of S. dulcamara during the transition from mature green (MG) to ripe red (RR) developmental stage. By combining untargeted (UHPLC/Orbitrap MSn) and targeted (UHPLC-DAD MS2) metabolomics approaches, we revealed a significant change in polyphenolic profile of S. dulcamara fruits during fruit ripening. In order to improve knowledge on the link between metabolism and cell redox status, Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) of reactive oxygen species (ROS) in intact fruits, fruit sections and extracts, were performed in parallel with the analysis of tissue-specific distribution of antioxidants and activity of antioxidant enzymes, which demonstrated alterations in the redox state and the increase of total antioxidant capacity of bittersweet berries during ripening, especially of outer layers (pericarp) of the fruits. This knowledge could provide important information to direct research on fruit quality improvement of flood-intolerant crops that are a close relatives of bittersweet, such as tomato and eggplant.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations",
pages = "126",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5270"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Gašić, U., Živković, S., Savić, J., Skorić, M., Devrnja, N., Banjanac, T., Todorović, M., Mojović, M.,& Mišić, D.. (2022). Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 126.
https://hdl.handle.net/21.15107/rcub_ibiss_5270

Milutinović M, Nakarada Đ, Božunović J, Gašić U, Živković S, Savić J, Skorić M, Devrnja N, Banjanac T, Todorović M, Mojović M, Mišić D. Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:126.
https://hdl.handle.net/21.15107/rcub_ibiss_5270 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Gašić, Uroš, Živković, Suzana, Savić, Jelena, Skorić, Marijana, Devrnja, Nina, Banjanac, Tijana, Todorović, Miloš, Mojović, Miloš, Mišić, Danijela, "Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):126,
https://hdl.handle.net/21.15107/rcub_ibiss_5270 .

TY  - CONF
AU  - Šajkunić, Sanja
AU  - Dragićević, Milan
AU  - Milutinović, Milica
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Devrnja, Nina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5050
AB  - Different stressors from the environment can prime defense and enable enhanced responses
to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the
level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown
promising prospects as novel priming inducers. Still, little is known about their potential to induce
epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula
L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to
EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal.
Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM)
DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR,
and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and
treated samples were analyzed. The average methylation level of the whole genome was 72.29%
and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly
occurred in the CG context compared to CHH and CHG. In general, hypomethylated
sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially
methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein
processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes
associated with these pathways, as well as different defense responses in potato (GO:0006952),
will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress
conditions.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - French marigold essential oil affected DNA methylation in potato
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5050
ER  - 

@conference{
author = "Šajkunić, Sanja and Dragićević, Milan and Milutinović, Milica and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena and Devrnja, Nina",
year = "2022",
abstract = "Different stressors from the environment can prime defense and enable enhanced responses
to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the
level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown
promising prospects as novel priming inducers. Still, little is known about their potential to induce
epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula
L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to
EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal.
Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM)
DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR,
and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and
treated samples were analyzed. The average methylation level of the whole genome was 72.29%
and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly
occurred in the CG context compared to CHH and CHG. In general, hypomethylated
sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially
methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein
processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes
associated with these pathways, as well as different defense responses in potato (GO:0006952),
will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress
conditions.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "French marigold essential oil affected DNA methylation in potato",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5050"
}

Šajkunić, S., Dragićević, M., Milutinović, M., Stupar, S., Tubić, L., Savić, J.,& Devrnja, N.. (2022). French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 71.
https://hdl.handle.net/21.15107/rcub_ibiss_5050

Šajkunić S, Dragićević M, Milutinović M, Stupar S, Tubić L, Savić J, Devrnja N. French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:71.
https://hdl.handle.net/21.15107/rcub_ibiss_5050 .

Šajkunić, Sanja, Dragićević, Milan, Milutinović, Milica, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, Devrnja, Nina, "French marigold essential oil affected DNA methylation in potato" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):71,
https://hdl.handle.net/21.15107/rcub_ibiss_5050 .

TY  - CONF
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Anđelković, Boban
AU  - Tešević, Vele
AU  - Milutinović, Milica
AU  - Skorić, Marijana
AU  - Savić, Jelena
PY  - 2022
UR  - https://www.plantslo.org/wp/wp-content/uploads/2022/09/PlantsInChangingEnvironment22AbstractBook.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5054
AB  - Essential oils (EOs) obtained from plants can frequently exhibit toxicity and repellency/deterrence against insect pests. Fennel (Foeniculum vulgare Mill.) and basil (Ocimum basilicum L.) EOs are used in pest management applications, but the most-discussed constraint regards restraining the usage of these natural insecticides in common agricultural practices is related to the volatility and limited persistence of EOs under field conditions. Due to high volatility, the efficacy of these substances falls short after EO application and requires frequent reapplication. There is a great interest in developing formulations that improve the handling of EOs, prevent the photodegradation of their active ingredients, and control the release of volatiles through micro/nanospheres or capsules in solid or liquid form. The polymer-based EO nanoformulations provide the stability and optimal effectiveness of EOs with an anti-insecticidal effect. One of the main advantages of solid formulations is that they can have a longer shelf life and be applied in solid form or even mixed with water and sprayed. We investigated the formation and characterization of inclusion complexes (ICs) of beta-cyclodextrin (β-CD) with two selected EOs, using the co-precipitation method. The main constituents of EOs determined by GC-MS analyses were trans-anethole (37.77%) and α-pinene (23.51%) in fennel EO, and linalool (32.6%) and methyl chavicol (23.2%) in basil EO. The interaction between β-CD and EO molecules was assessed through Fourier-transform infrared spectroscopy (FT-IR) and it was used to characterize the ICs by evaluating the changes recorded in the ICs compared to the free β-CD and individual EO. FT-IR spectra of inclusion complexes displayed peak shifts and variations of intensity when it compared to FT-IR spectra of EOs and free β-CD. β-cyclodextrin resulted as an effective host in encapsulating selected EOs. The potato plants sprayed with ICs were examined by scanning electron microscopy (SEM) and showed ICs with lamellate or rhomboid morphology. Bioassay with Colorado potato beetle fed with potato plants covered with ICs showed that complexation with β-CD reduced insects’ growth (body mass of IV instar larvae where 119.84±1.48 mg and 120.79±7.42 mg for IC with fennel and basil EO respectively in comparison to body mass of control larvae 138.97±2.01mg) and affected digestive proteolytic activity.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth
SP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5054
ER  - 

@conference{
author = "Devrnja, Nina and Šajkunić, Sanja and Anđelković, Boban and Tešević, Vele and Milutinović, Milica and Skorić, Marijana and Savić, Jelena",
year = "2022",
abstract = "Essential oils (EOs) obtained from plants can frequently exhibit toxicity and repellency/deterrence against insect pests. Fennel (Foeniculum vulgare Mill.) and basil (Ocimum basilicum L.) EOs are used in pest management applications, but the most-discussed constraint regards restraining the usage of these natural insecticides in common agricultural practices is related to the volatility and limited persistence of EOs under field conditions. Due to high volatility, the efficacy of these substances falls short after EO application and requires frequent reapplication. There is a great interest in developing formulations that improve the handling of EOs, prevent the photodegradation of their active ingredients, and control the release of volatiles through micro/nanospheres or capsules in solid or liquid form. The polymer-based EO nanoformulations provide the stability and optimal effectiveness of EOs with an anti-insecticidal effect. One of the main advantages of solid formulations is that they can have a longer shelf life and be applied in solid form or even mixed with water and sprayed. We investigated the formation and characterization of inclusion complexes (ICs) of beta-cyclodextrin (β-CD) with two selected EOs, using the co-precipitation method. The main constituents of EOs determined by GC-MS analyses were trans-anethole (37.77%) and α-pinene (23.51%) in fennel EO, and linalool (32.6%) and methyl chavicol (23.2%) in basil EO. The interaction between β-CD and EO molecules was assessed through Fourier-transform infrared spectroscopy (FT-IR) and it was used to characterize the ICs by evaluating the changes recorded in the ICs compared to the free β-CD and individual EO. FT-IR spectra of inclusion complexes displayed peak shifts and variations of intensity when it compared to FT-IR spectra of EOs and free β-CD. β-cyclodextrin resulted as an effective host in encapsulating selected EOs. The potato plants sprayed with ICs were examined by scanning electron microscopy (SEM) and showed ICs with lamellate or rhomboid morphology. Bioassay with Colorado potato beetle fed with potato plants covered with ICs showed that complexation with β-CD reduced insects’ growth (body mass of IV instar larvae where 119.84±1.48 mg and 120.79±7.42 mg for IC with fennel and basil EO respectively in comparison to body mass of control larvae 138.97±2.01mg) and affected digestive proteolytic activity.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth",
pages = "7",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5054"
}

Devrnja, N., Šajkunić, S., Anđelković, B., Tešević, V., Milutinović, M., Skorić, M.,& Savić, J.. (2022). Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Ljubljana: Slovenian Society of Plant Biology., 7.
https://hdl.handle.net/21.15107/rcub_ibiss_5054

Devrnja N, Šajkunić S, Anđelković B, Tešević V, Milutinović M, Skorić M, Savić J. Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:7.
https://hdl.handle.net/21.15107/rcub_ibiss_5054 .

Devrnja, Nina, Šajkunić, Sanja, Anđelković, Boban, Tešević, Vele, Milutinović, Milica, Skorić, Marijana, Savić, Jelena, "Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth" in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):7,
https://hdl.handle.net/21.15107/rcub_ibiss_5054 .

TY  - CONF
AU  - Šajkunić, Sanja
AU  - Dragićević, Milan
AU  - Milutinović, Milica
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Devrnja, Nina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5051
AB  - Different stressors from the environment can prime defense and enable enhanced responses to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown promising prospects as novel priming inducers. Still, little is known about their potential to induce epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM) DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and treated samples were analyzed. The average methylation level of the whole genome was 72.29% and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly occurred in the CG context compared to CHH and CHG. In general, hypomethylated sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes associated with these pathways, as well as different defense responses in potato (GO:0006952), will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress conditions.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.
T1  - French marigold essential oil affected DNA methylation in potato
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5051
ER  - 

@conference{
author = "Šajkunić, Sanja and Dragićević, Milan and Milutinović, Milica and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena and Devrnja, Nina",
year = "2022",
abstract = "Different stressors from the environment can prime defense and enable enhanced responses to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown promising prospects as novel priming inducers. Still, little is known about their potential to induce epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM) DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and treated samples were analyzed. The average methylation level of the whole genome was 72.29% and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly occurred in the CG context compared to CHH and CHG. In general, hypomethylated sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes associated with these pathways, as well as different defense responses in potato (GO:0006952), will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress conditions.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.",
title = "French marigold essential oil affected DNA methylation in potato",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5051"
}

Šajkunić, S., Dragićević, M., Milutinović, M., Stupar, S., Tubić, L., Savić, J.,& Devrnja, N.. (2022). French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5051

Šajkunić S, Dragićević M, Milutinović M, Stupar S, Tubić L, Savić J, Devrnja N. French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5051 .

Šajkunić, Sanja, Dragićević, Milan, Milutinović, Milica, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, Devrnja, Nina, "French marigold essential oil affected DNA methylation in potato" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5051 .

TY  - CONF
AU  - Savić, Jelena
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Tubić, Ljiljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5053
AB  - Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Role of glutathione in potato response to French marigold essential oil
SP  - P6
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5053
ER  - 

@conference{
author = "Savić, Jelena and Stupar, Sofija and Devrnja, Nina and Šajkunić, Sanja and Tubić, Ljiljana",
year = "2022",
abstract = "Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Role of glutathione in potato response to French marigold essential oil",
pages = "P6",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5053"
}

Savić, J., Stupar, S., Devrnja, N., Šajkunić, S.,& Tubić, L.. (2022). Role of glutathione in potato response to French marigold essential oil. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., P6.
https://hdl.handle.net/21.15107/rcub_ibiss_5053

Savić J, Stupar S, Devrnja N, Šajkunić S, Tubić L. Role of glutathione in potato response to French marigold essential oil. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:P6.
https://hdl.handle.net/21.15107/rcub_ibiss_5053 .

Savić, Jelena, Stupar, Sofija, Devrnja, Nina, Šajkunić, Sanja, Tubić, Ljiljana, "Role of glutathione in potato response to French marigold essential oil" in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):P6,
https://hdl.handle.net/21.15107/rcub_ibiss_5053 .

TY  - CONF
AU  - Savić, Jelena
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Tubić, Ljiljana
PY  - 2022
UR  - https://www.plantslo.org/wp/wp-content/uploads/2022/09/PlantsInChangingEnvironment22AbstractBook.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5052
AB  - Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Role of glutathione in potato response to French marigold essential oil
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5052
ER  - 

@conference{
author = "Savić, Jelena and Stupar, Sofija and Devrnja, Nina and Šajkunić, Sanja and Tubić, Ljiljana",
year = "2022",
abstract = "Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Role of glutathione in potato response to French marigold essential oil",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5052"
}

Savić, J., Stupar, S., Devrnja, N., Šajkunić, S.,& Tubić, L.. (2022). Role of glutathione in potato response to French marigold essential oil. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Ljubljana: Slovenian Society of Plant Biology., 17.
https://hdl.handle.net/21.15107/rcub_ibiss_5052

Savić J, Stupar S, Devrnja N, Šajkunić S, Tubić L. Role of glutathione in potato response to French marigold essential oil. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:17.
https://hdl.handle.net/21.15107/rcub_ibiss_5052 .

Savić, Jelena, Stupar, Sofija, Devrnja, Nina, Šajkunić, Sanja, Tubić, Ljiljana, "Role of glutathione in potato response to French marigold essential oil" in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):17,
https://hdl.handle.net/21.15107/rcub_ibiss_5052 .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragićević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Perišić, Mirjana
AU  - Mišić, Danijela
PY  - 2021
UR  - https://doi.org/10.3390/plants10010142
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4232
AB  - Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.
PB  - MDPI AG
T2  - Plants
T1  - Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro
IS  - 1
VL  - 10
DO  - 10.3390/plants10010142
SP  - 1
EP  - 20
ER  - 

@article{
author = "Dmitrović, Slavica and Dragićević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Perišić, Mirjana and Mišić, Danijela",
year = "2021",
abstract = "Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.",
publisher = "MDPI AG",
journal = "Plants",
title = "Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro",
number = "1",
volume = "10",
doi = "10.3390/plants10010142",
pages = "1-20"
}

Dmitrović, S., Dragićević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D., Perišić, M.,& Mišić, D.. (2021). Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro. in Plants
MDPI AG., 10(1), 1-20.
https://doi.org/10.3390/plants10010142

Dmitrović S, Dragićević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Perišić M, Mišić D. Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro. in Plants. 2021;10(1):1-20.
doi:10.3390/plants10010142 .

Dmitrović, Slavica, Dragićević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Perišić, Mirjana, Mišić, Danijela, "Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro" in Plants, 10, no. 1 (2021):1-20,
https://doi.org/10.3390/plants10010142 . .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Stanković-Jeremić, Jovana
AU  - Maksimović, Vuk
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - internal-pdf://Stupar et al. - 2021 - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf St.pdf
UR  - https://www.mdpi.com/2223-7747/10/1/172
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4147
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI AG
T2  - Plants
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
SP  - 172
ER  - 

@article{
author = "Stupar, Sofija and Dragićević, Milan and Tešević, Vele and Stanković-Jeremić, Jovana and Maksimović, Vuk and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI AG",
journal = "Plants",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172",
pages = "172"
}

Stupar, S., Dragićević, M., Tešević, V., Stanković-Jeremić, J., Maksimović, V., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants
MDPI AG., 10(1), 172.
https://doi.org/10.3390/plants10010172

Stupar S, Dragićević M, Tešević V, Stanković-Jeremić J, Maksimović V, Ćosić T, Devrnja N, Tubić L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants. 2021;10(1):172.
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragićević, Milan, Tešević, Vele, Stanković-Jeremić, Jovana, Maksimović, Vuk, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants, 10, no. 1 (2021):172,
https://doi.org/10.3390/plants10010172 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Marković, Marija
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2021
UR  - https://www.nature.com/articles/s41598-021-85932-w
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4194
AB  - Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.
PB  - Springer Science and Business Media LLC
T2  - Scientific Reports
T1  - Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-85932-w
SP  - 6494
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Václav and Savić, Jelena and Raspor, Martin and Marković, Marija and Dobrev, Petre I. and Ninković, Slavica",
year = "2021",
abstract = "Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.",
publisher = "Springer Science and Business Media LLC",
journal = "Scientific Reports",
title = "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-85932-w",
pages = "6494"
}

Ćosić, T., Motyka, V., Savić, J., Raspor, M., Marković, M., Dobrev, P. I.,& Ninković, S.. (2021). Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports
Springer Science and Business Media LLC., 11(1), 6494.
https://doi.org/10.1038/s41598-021-85932-w

Ćosić T, Motyka V, Savić J, Raspor M, Marković M, Dobrev PI, Ninković S. Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports. 2021;11(1):6494.
doi:10.1038/s41598-021-85932-w .

Ćosić, Tatjana, Motyka, Václav, Savić, Jelena, Raspor, Martin, Marković, Marija, Dobrev, Petre I., Ninković, Slavica, "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi" in Scientific Reports, 11, no. 1 (2021):6494,
https://doi.org/10.1038/s41598-021-85932-w . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Krstić Milošević, Dijana
AU  - Janošević, Dušica
AU  - Tešević, Vele
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
AU  - Ćalić, Dušica
PY  - 2021
UR  - http://link.springer.com/10.1007/s00709-020-01588-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4038
AB  - In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.
PB  - Springer
T2  - Protoplasma
T1  - In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents
IS  - 3
VL  - 258
DO  - 10.1007/s00709-020-01588-9
SP  - 587
EP  - 599
ER  - 

@article{
author = "Devrnja, Nina and Krstić Milošević, Dijana and Janošević, Dušica and Tešević, Vele and Vinterhalter, Branka and Savić, Jelena and Ćalić, Dušica",
year = "2021",
abstract = "In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.",
publisher = "Springer",
journal = "Protoplasma",
title = "In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents",
number = "3",
volume = "258",
doi = "10.1007/s00709-020-01588-9",
pages = "587-599"
}

Devrnja, N., Krstić Milošević, D., Janošević, D., Tešević, V., Vinterhalter, B., Savić, J.,& Ćalić, D.. (2021). In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents. in Protoplasma
Springer., 258(3), 587-599.
https://doi.org/10.1007/s00709-020-01588-9

Devrnja N, Krstić Milošević D, Janošević D, Tešević V, Vinterhalter B, Savić J, Ćalić D. In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents. in Protoplasma. 2021;258(3):587-599.
doi:10.1007/s00709-020-01588-9 .

Devrnja, Nina, Krstić Milošević, Dijana, Janošević, Dušica, Tešević, Vele, Vinterhalter, Branka, Savić, Jelena, Ćalić, Dušica, "In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents" in Protoplasma, 258, no. 3 (2021):587-599,
https://doi.org/10.1007/s00709-020-01588-9 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Savić, Jelena
AU  - Ćalić, Dušica
PY  - 2020
UR  - internal-pdf://Devrnja et al. - 2020 - Evaluation of tansy essential oil as a potential “green” alternative for gypsy moth control.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3601
AB  - The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality (< 10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet (digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.
T2  - Environmental Science and Pollution Research
T1  - Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.
VL  - 27
DO  - 10.1007/s11356-020-07825-1
SP  - 11958
EP  - 11967
ER  - 

@article{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Savić, Jelena and Ćalić, Dušica",
year = "2020",
abstract = "The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality (< 10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet (digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.",
journal = "Environmental Science and Pollution Research",
title = "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.",
volume = "27",
doi = "10.1007/s11356-020-07825-1",
pages = "11958-11967"
}

Devrnja, N., Kostić, I., Lazarević, J., Savić, J.,& Ćalić, D.. (2020). Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.. in Environmental Science and Pollution Research, 27, 11958-11967.
https://doi.org/10.1007/s11356-020-07825-1

Devrnja N, Kostić I, Lazarević J, Savić J, Ćalić D. Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.. in Environmental Science and Pollution Research. 2020;27:11958-11967.
doi:10.1007/s11356-020-07825-1 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Savić, Jelena, Ćalić, Dušica, "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control." in Environmental Science and Pollution Research, 27 (2020):11958-11967,
https://doi.org/10.1007/s11356-020-07825-1 . .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Savić, Jelena
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Girek, Zdenka
AU  - Adžić, Slađan
AU  - Zečević, Bogoljub
AU  - Banjac, Nevena
PY  - 2020
UR  - https://doi.org/10.1007/s11240-020-01948-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4011
AB  - Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression of NPK1 transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. The Agrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring the NPK1 and phosphinothricin N-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of both NPK1 and bar transgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level of NPK1 gene expression estimated by RT-qPCR, and the L2 line with the highest NPK1 expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of the bar gene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field. Key message The results underlined that constitutively expressing NPK1 can significantly contribute to enhanced salt stresstolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerancecruciferous vegetable lines.
PB  - Springer Science and Business Media LLC
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower
IS  - 3
VL  - 143
DO  - 10.1007/s11240-020-01948-6
SP  - 635
EP  - 651
ER  - 

@article{
author = "Pavlović, Suzana and Savić, Jelena and Milojević, Jelena and Vinterhalter, Branka and Girek, Zdenka and Adžić, Slađan and Zečević, Bogoljub and Banjac, Nevena",
year = "2020",
abstract = "Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression of NPK1 transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. The Agrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring the NPK1 and phosphinothricin N-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of both NPK1 and bar transgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level of NPK1 gene expression estimated by RT-qPCR, and the L2 line with the highest NPK1 expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of the bar gene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field. Key message The results underlined that constitutively expressing NPK1 can significantly contribute to enhanced salt stresstolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerancecruciferous vegetable lines.",
publisher = "Springer Science and Business Media LLC",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower",
number = "3",
volume = "143",
doi = "10.1007/s11240-020-01948-6",
pages = "635-651"
}

Pavlović, S., Savić, J., Milojević, J., Vinterhalter, B., Girek, Z., Adžić, S., Zečević, B.,& Banjac, N.. (2020). Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell, Tissue and Organ Culture (PCTOC)
Springer Science and Business Media LLC., 143(3), 635-651.
https://doi.org/10.1007/s11240-020-01948-6

Pavlović S, Savić J, Milojević J, Vinterhalter B, Girek Z, Adžić S, Zečević B, Banjac N. Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell, Tissue and Organ Culture (PCTOC). 2020;143(3):635-651.
doi:10.1007/s11240-020-01948-6 .

Pavlović, Suzana, Savić, Jelena, Milojević, Jelena, Vinterhalter, Branka, Girek, Zdenka, Adžić, Slađan, Zečević, Bogoljub, Banjac, Nevena, "Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower" in Plant Cell, Tissue and Organ Culture (PCTOC), 143, no. 3 (2020):635-651,
https://doi.org/10.1007/s11240-020-01948-6 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Cingel, Aleksandar
AU  - Ghalawnji, Nabil
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3816
AB  - Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of
the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators
(PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were
studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18±2°C on half-strength
MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose
and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA),
gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all
treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a
distinctive manner, with a variety of morphological traits being altered in comparison to matching
controls.
PB  - Serbian Biological Socitey
T2  - Archives of Biological Sciences
T1  - Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro
IS  - 3
VL  - 72
DO  - 10.2298/ABS200622029C
SP  - 349
EP  - 357
ER  - 

@article{
author = "Ćosić, Tatjana and Savić, Jelena and Raspor, Martin and Cingel, Aleksandar and Ghalawnji, Nabil and Vinterhalter, Branka and Ninković, Slavica",
year = "2020",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of
the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators
(PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were
studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18±2°C on half-strength
MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose
and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA),
gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all
treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a
distinctive manner, with a variety of morphological traits being altered in comparison to matching
controls.",
publisher = "Serbian Biological Socitey",
journal = "Archives of Biological Sciences",
title = "Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro",
number = "3",
volume = "72",
doi = "10.2298/ABS200622029C",
pages = "349-357"
}

Ćosić, T., Savić, J., Raspor, M., Cingel, A., Ghalawnji, N., Vinterhalter, B.,& Ninković, S.. (2020). Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. in Archives of Biological Sciences
Serbian Biological Socitey., 72(3), 349-357.
https://doi.org/10.2298/ABS200622029C

Ćosić T, Savić J, Raspor M, Cingel A, Ghalawnji N, Vinterhalter B, Ninković S. Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. in Archives of Biological Sciences. 2020;72(3):349-357.
doi:10.2298/ABS200622029C .

Ćosić, Tatjana, Savić, Jelena, Raspor, Martin, Cingel, Aleksandar, Ghalawnji, Nabil, Vinterhalter, Branka, Ninković, Slavica, "Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro" in Archives of Biological Sciences, 72, no. 3 (2020):349-357,
https://doi.org/10.2298/ABS200622029C . .

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Janošević, Dušica
AU  - Savić, Jelena
AU  - Todorović, Slađana
AU  - Banjac, Nevena
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3774
AB  - Gibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 µM α-naphthaleneacetic acid (NAA) and 0–10 µM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1 and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1 and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expression levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.
PB  - Springer
T2  - Plant Cell, Tissue and Organ Culture
T1  - Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach
VL  - 142
DO  - 10.1007/s11240-020-01878-3
SP  - 537
EP  - 548
ER  - 

@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Janošević, Dušica and Savić, Jelena and Todorović, Slađana and Banjac, Nevena and Milojević, Jelena",
year = "2020",
abstract = "Gibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 µM α-naphthaleneacetic acid (NAA) and 0–10 µM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1 and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1 and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expression levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.",
publisher = "Springer",
journal = "Plant Cell, Tissue and Organ Culture",
title = "Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach",
volume = "142",
doi = "10.1007/s11240-020-01878-3",
pages = "537-548"
}

Belić, M., Zdravković-Korać, S., Janošević, D., Savić, J., Todorović, S., Banjac, N.,& Milojević, J.. (2020). Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach. in Plant Cell, Tissue and Organ Culture
Springer., 142, 537-548.
https://doi.org/10.1007/s11240-020-01878-3

Belić M, Zdravković-Korać S, Janošević D, Savić J, Todorović S, Banjac N, Milojević J. Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach. in Plant Cell, Tissue and Organ Culture. 2020;142:537-548.
doi:10.1007/s11240-020-01878-3 .

Belić, Maja, Zdravković-Korać, Snežana, Janošević, Dušica, Savić, Jelena, Todorović, Slađana, Banjac, Nevena, Milojević, Jelena, "Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach" in Plant Cell, Tissue and Organ Culture, 142 (2020):537-548,
https://doi.org/10.1007/s11240-020-01878-3 . .