TY  - DATA
AU  - Milanović, Slobodan
AU  - Milenković, Ivan
AU  - Lazarević, Jelica
AU  - Todosijević, Marina
AU  - Ljujić, Jovana
AU  - Mitić, Zorica
AU  - Nikolić, Biljana
AU  - Marin, Petar
AU  - Tešević, Vele
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6490
AB  - We provide one excel (with five sheets) and 5 csv files. The data in the excel file is the same as the one provided in the 5 csv files. Data file named 'raw data.xlsx' is consisted of 5 sheets containing raw data on all analyzed traits in the manuscript for the spongy moth and Phytophthora sp. The first sheet named 'RDC' contains data on relative deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The second sheet named 'ADC' contains data on absolute deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The third sheet named 'RCR' contains data on the relative consumption rate of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The forth sheet named ‘RGR’ contains data on relative growth rate of gypsy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The fifth sheet named ‘Ir’ contains data on the antimicrobial activity of three concentrations of Calocedrus decurrens and Cupressus arizonica essential oils against Phytophthora × cambivora, P. plurivora and P. quercina expressed as inhibition rate of mycelial growth.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6490
ER  - 

@misc{
author = "Milanović, Slobodan and Milenković, Ivan and Lazarević, Jelica and Todosijević, Marina and Ljujić, Jovana and Mitić, Zorica and Nikolić, Biljana and Marin, Petar and Tešević, Vele",
year = "2024",
abstract = "We provide one excel (with five sheets) and 5 csv files. The data in the excel file is the same as the one provided in the 5 csv files. Data file named 'raw data.xlsx' is consisted of 5 sheets containing raw data on all analyzed traits in the manuscript for the spongy moth and Phytophthora sp. The first sheet named 'RDC' contains data on relative deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The second sheet named 'ADC' contains data on absolute deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The third sheet named 'RCR' contains data on the relative consumption rate of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The forth sheet named ‘RGR’ contains data on relative growth rate of gypsy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The fifth sheet named ‘Ir’ contains data on the antimicrobial activity of three concentrations of Calocedrus decurrens and Cupressus arizonica essential oils against Phytophthora × cambivora, P. plurivora and P. quercina expressed as inhibition rate of mycelial growth.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6490"
}

Milanović, S., Milenković, I., Lazarević, J., Todosijević, M., Ljujić, J., Mitić, Z., Nikolić, B., Marin, P.,& Tešević, V.. (2024). Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6490

Milanović S, Milenković I, Lazarević J, Todosijević M, Ljujić J, Mitić Z, Nikolić B, Marin P, Tešević V. Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_6490 .

Milanović, Slobodan, Milenković, Ivan, Lazarević, Jelica, Todosijević, Marina, Ljujić, Jovana, Mitić, Zorica, Nikolić, Biljana, Marin, Petar, Tešević, Vele, "Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens" (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_6490 .

TY  - JOUR
AU  - Scanavacca, Juliana
AU  - Bortolucci, Wanessa de Campos
AU  - Jacomassi, Ezilda
AU  - Baretta, Irineia Paulina
AU  - Faria, Maria Graciela Iecher
AU  - Fernandez, Carla Maria Mariano
AU  - Tešević, Vele
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Colauto, Nelson Barros
AU  - Linde, Giani Andrea
AU  - Gazim, Zilda Cristiani
PY  - 2023
UR  - https://blacpma.ms-editions.cl/index.php/blacpma/article/view/337/345
UR  - https://blacpma.ms-editions.cl/index.php/blacpma/article/view/337
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5233
AB  - Food spoilage is a widely neglected problem and the constant use of synthetic fungicides could develop resistant fungi. The objective of this study was to evaluate the chemical composition and antimicrobial activity of Tetradenia riparia leaf essential oil against foodborne disease microorganisms. Leaf essential oil was obtained by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antimicrobial activity was studied by broth microdilution. The major compounds identified were oxygenated sesquiterpenes (43.6%): 14-hydroxy-9-epi-(E)-cariophylene (20.8%) and τ-cadinol (18.4%); followed by oxygenated diterpenes (24.6%): 6,7-dehydroroyleanone (12.6%) and 9β, 13β-epoxy-7-abiethene (10.6%); sesquiterpenic hydrocarbons (17.1%) and oxygenated monoterpenes (7.4%): fenchone (5.6%). The essential oil had broad antibacterial and antifungal activity, mainly against A. versicolor and P. ochrochloron with fungistatic and fungicidal activities and B. cereus, L. monocytogenes, and S. aureus with bacteriostatic and bactericidal activities. T. riparia leaf essential oil is a potential alternative to control microorganisms.
PB  - MS-Editions
T2  - Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
T1  - Antimicrobial activity of Tetradenia riparia leaf essential oil
IS  - 2
VL  - 22
DO  - 10.37360/blacpma.23.22.2.19
SP  - 255
EP  - 267
ER  - 

@article{
author = "Scanavacca, Juliana and Bortolucci, Wanessa de Campos and Jacomassi, Ezilda and Baretta, Irineia Paulina and Faria, Maria Graciela Iecher and Fernandez, Carla Maria Mariano and Tešević, Vele and Glamočlija, Jasmina and Soković, Marina and Colauto, Nelson Barros and Linde, Giani Andrea and Gazim, Zilda Cristiani",
year = "2023",
abstract = "Food spoilage is a widely neglected problem and the constant use of synthetic fungicides could develop resistant fungi. The objective of this study was to evaluate the chemical composition and antimicrobial activity of Tetradenia riparia leaf essential oil against foodborne disease microorganisms. Leaf essential oil was obtained by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antimicrobial activity was studied by broth microdilution. The major compounds identified were oxygenated sesquiterpenes (43.6%): 14-hydroxy-9-epi-(E)-cariophylene (20.8%) and τ-cadinol (18.4%); followed by oxygenated diterpenes (24.6%): 6,7-dehydroroyleanone (12.6%) and 9β, 13β-epoxy-7-abiethene (10.6%); sesquiterpenic hydrocarbons (17.1%) and oxygenated monoterpenes (7.4%): fenchone (5.6%). The essential oil had broad antibacterial and antifungal activity, mainly against A. versicolor and P. ochrochloron with fungistatic and fungicidal activities and B. cereus, L. monocytogenes, and S. aureus with bacteriostatic and bactericidal activities. T. riparia leaf essential oil is a potential alternative to control microorganisms.",
publisher = "MS-Editions",
journal = "Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas",
title = "Antimicrobial activity of Tetradenia riparia leaf essential oil",
number = "2",
volume = "22",
doi = "10.37360/blacpma.23.22.2.19",
pages = "255-267"
}

Scanavacca, J., Bortolucci, W. d. C., Jacomassi, E., Baretta, I. P., Faria, M. G. I., Fernandez, C. M. M., Tešević, V., Glamočlija, J., Soković, M., Colauto, N. B., Linde, G. A.,& Gazim, Z. C.. (2023). Antimicrobial activity of Tetradenia riparia leaf essential oil. in Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
MS-Editions., 22(2), 255-267.
https://doi.org/10.37360/blacpma.23.22.2.19

Scanavacca J, Bortolucci WDC, Jacomassi E, Baretta IP, Faria MGI, Fernandez CMM, Tešević V, Glamočlija J, Soković M, Colauto NB, Linde GA, Gazim ZC. Antimicrobial activity of Tetradenia riparia leaf essential oil. in Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas. 2023;22(2):255-267.
doi:10.37360/blacpma.23.22.2.19 .

Scanavacca, Juliana, Bortolucci, Wanessa de Campos, Jacomassi, Ezilda, Baretta, Irineia Paulina, Faria, Maria Graciela Iecher, Fernandez, Carla Maria Mariano, Tešević, Vele, Glamočlija, Jasmina, Soković, Marina, Colauto, Nelson Barros, Linde, Giani Andrea, Gazim, Zilda Cristiani, "Antimicrobial activity of Tetradenia riparia leaf essential oil" in Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, 22, no. 2 (2023):255-267,
https://doi.org/10.37360/blacpma.23.22.2.19 . .

TY  - JOUR
AU  - Jadranin, Milka
AU  - Savić, Danica
AU  - Lupšić, Ema
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
AU  - Krstić, Gordana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6557
AB  - Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes' chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.
PB  - Basel: MDPI
T2  - Plants
T1  - LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity
IS  - 24
VL  - 12
DO  - 10.3390/plants12244181
SP  - 4181
ER  - 

@article{
author = "Jadranin, Milka and Savić, Danica and Lupšić, Ema and Podolski-Renić, Ana and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan and Krstić, Gordana",
year = "2023",
abstract = "Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes' chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity",
number = "24",
volume = "12",
doi = "10.3390/plants12244181",
pages = "4181"
}

Jadranin, M., Savić, D., Lupšić, E., Podolski-Renić, A., Pešić, M., Tešević, V., Milosavljević, S.,& Krstić, G.. (2023). LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants
Basel: MDPI., 12(24), 4181.
https://doi.org/10.3390/plants12244181

Jadranin M, Savić D, Lupšić E, Podolski-Renić A, Pešić M, Tešević V, Milosavljević S, Krstić G. LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants. 2023;12(24):4181.
doi:10.3390/plants12244181 .

Jadranin, Milka, Savić, Danica, Lupšić, Ema, Podolski-Renić, Ana, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan, Krstić, Gordana, "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity" in Plants, 12, no. 24 (2023):4181,
https://doi.org/10.3390/plants12244181 . .

TY  - CONF
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Jovanović Stojanov, Sofija
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6594
AB  - In this research, from two species, E. palustris and E. lucida, four ingenane derivatives were isolated. Their anticancer effects were evaluated in the human melanoma – 518A2 cell line and compared with the effects of ingenolmebutate. Selectivity towards human melanoma cells was determined using normal human keratinocytes – HaCaT.
PB  - Skopje: Macedonian Pharmaceutical Association
C3  - 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia
T1  - Anti-melanoma effects of ingenanes isolated from Euphorbia species
DO  - 10.33320/maced.pharm.bull.2022.68.04.006
SP  - 23
EP  - 24
ER  - 

@conference{
author = "Krstić, Gordana and Jadranin, Milka and Jovanović Stojanov, Sofija and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan",
year = "2022",
abstract = "In this research, from two species, E. palustris and E. lucida, four ingenane derivatives were isolated. Their anticancer effects were evaluated in the human melanoma – 518A2 cell line and compared with the effects of ingenolmebutate. Selectivity towards human melanoma cells was determined using normal human keratinocytes – HaCaT.",
publisher = "Skopje: Macedonian Pharmaceutical Association",
journal = "11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia",
title = "Anti-melanoma effects of ingenanes isolated from Euphorbia species",
doi = "10.33320/maced.pharm.bull.2022.68.04.006",
pages = "23-24"
}

Krstić, G., Jadranin, M., Jovanović Stojanov, S., Pešić, M., Tešević, V.,& Milosavljević, S.. (2022). Anti-melanoma effects of ingenanes isolated from Euphorbia species. in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia
Skopje: Macedonian Pharmaceutical Association., 23-24.
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.006

Krstić G, Jadranin M, Jovanović Stojanov S, Pešić M, Tešević V, Milosavljević S. Anti-melanoma effects of ingenanes isolated from Euphorbia species. in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia. 2022;:23-24.
doi:10.33320/maced.pharm.bull.2022.68.04.006 .

Krstić, Gordana, Jadranin, Milka, Jovanović Stojanov, Sofija, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan, "Anti-melanoma effects of ingenanes isolated from Euphorbia species" in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia (2022):23-24,
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.006 . .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Ćirić, Ana
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Anđelković, Boban
AU  - Todosijević, Marina
AU  - Todorović, Slađana
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Tešević, Vele
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Kanellis, Angelos K.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669022004757
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4983
AB  - Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites , such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus . Histochemical staining of leaves' trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus , grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) and two Gram-positive bacteria ( Bacillus cereus and Micrococcus flavus ). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent -3 β -acetoxy-13- epi -manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent -3 β -acetoxy-13- epi -manoyl oxide and its precursor ent -3 β -hydroxy-13- epi -manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”
VL  - 184
DO  - 10.1016/j.indcrop.2022.114992
SP  - 114992
ER  - 

@article{
author = "Skorić, Marijana and Ćirić, Ana and Budimir, Snežana and Janošević, Dušica and Anđelković, Boban and Todosijević, Marina and Todorović, Slađana and Soković, Marina and Glamočlija, Jasmina and Tešević, Vele and Gašić, Uroš and Mišić, Danijela and Kanellis, Angelos K.",
year = "2022",
abstract = "Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites , such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus . Histochemical staining of leaves' trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus , grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) and two Gram-positive bacteria ( Bacillus cereus and Micrococcus flavus ). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent -3 β -acetoxy-13- epi -manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent -3 β -acetoxy-13- epi -manoyl oxide and its precursor ent -3 β -hydroxy-13- epi -manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”",
volume = "184",
doi = "10.1016/j.indcrop.2022.114992",
pages = "114992"
}

Skorić, M., Ćirić, A., Budimir, S., Janošević, D., Anđelković, B., Todosijević, M., Todorović, S., Soković, M., Glamočlija, J., Tešević, V., Gašić, U., Mišić, D.,& Kanellis, A. K.. (2022). Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products
Elsevier B.V.., 184, 114992.
https://doi.org/10.1016/j.indcrop.2022.114992

Skorić M, Ćirić A, Budimir S, Janošević D, Anđelković B, Todosijević M, Todorović S, Soković M, Glamočlija J, Tešević V, Gašić U, Mišić D, Kanellis AK. Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products. 2022;184:114992.
doi:10.1016/j.indcrop.2022.114992 .

Skorić, Marijana, Ćirić, Ana, Budimir, Snežana, Janošević, Dušica, Anđelković, Boban, Todosijević, Marina, Todorović, Slađana, Soković, Marina, Glamočlija, Jasmina, Tešević, Vele, Gašić, Uroš, Mišić, Danijela, Kanellis, Angelos K., "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”" in Industrial Crops and Products, 184 (2022):114992,
https://doi.org/10.1016/j.indcrop.2022.114992 . .

TY  - JOUR
AU  - Llupa, Jonilda
AU  - Gašić, Uroš
AU  - Brčeski, Ilija
AU  - Demertzis, Panagiotis
AU  - Tešević, Vele
AU  - Topi, Dritan
PY  - 2022
UR  - http://www.agricultforest.ac.me/paper.php?id=3127
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5086
AB  - Quince and sweet cherry are two common edible fruit-producing trees of temperate regions of Euroasia. The phenolic profiles of juices from quince and sweet cherry fruit extracted by the cold-pressing were analyzed by Ultra-High-Performance Liquid Chromatography (UHPLC). Chromatographical results identified 31 phenolic compounds while quantified thirteen for fruit juices. The quantitative data indicate that the predominant phenolic was 5-O-caffeoylquinic acid in quince (8.343 mg L-1) and cherry juice (6.407 mg L-1). Total Flavonoids (TF), Total Phenolic Content (TPC), and Total Antioxidant Capacity (TAC) were also investigated. In both fruit juices, the TF values varied between 0.30-0.97 g L-1 (Catechin Equivalent) and TPC between 0.36 to 0.94 g L-1 (Gallic Acid Equivalent). It is worth noting that the quince fruit juices possessed a higher TPC and TFC compared to sweet cherry fruit juices. © 2022, University of Montenegro. All rights reserved.
PB  - Biotechnical Faculty of the University of Montenegro
T2  - Agriculture and Forestry
T1  - LC-MS/MS characterization of phenolic compounds in the quince (Cydonia oblonga Mill.) and sweet cherry (Prunus avium L.) fruit juices
IS  - 2
VL  - 68
DO  - 10.17707/AgricultForest.68.2.14
SP  - 193
EP  - 205
ER  - 

@article{
author = "Llupa, Jonilda and Gašić, Uroš and Brčeski, Ilija and Demertzis, Panagiotis and Tešević, Vele and Topi, Dritan",
year = "2022",
abstract = "Quince and sweet cherry are two common edible fruit-producing trees of temperate regions of Euroasia. The phenolic profiles of juices from quince and sweet cherry fruit extracted by the cold-pressing were analyzed by Ultra-High-Performance Liquid Chromatography (UHPLC). Chromatographical results identified 31 phenolic compounds while quantified thirteen for fruit juices. The quantitative data indicate that the predominant phenolic was 5-O-caffeoylquinic acid in quince (8.343 mg L-1) and cherry juice (6.407 mg L-1). Total Flavonoids (TF), Total Phenolic Content (TPC), and Total Antioxidant Capacity (TAC) were also investigated. In both fruit juices, the TF values varied between 0.30-0.97 g L-1 (Catechin Equivalent) and TPC between 0.36 to 0.94 g L-1 (Gallic Acid Equivalent). It is worth noting that the quince fruit juices possessed a higher TPC and TFC compared to sweet cherry fruit juices. © 2022, University of Montenegro. All rights reserved.",
publisher = "Biotechnical Faculty of the University of Montenegro",
journal = "Agriculture and Forestry",
title = "LC-MS/MS characterization of phenolic compounds in the quince (Cydonia oblonga Mill.) and sweet cherry (Prunus avium L.) fruit juices",
number = "2",
volume = "68",
doi = "10.17707/AgricultForest.68.2.14",
pages = "193-205"
}

Llupa, J., Gašić, U., Brčeski, I., Demertzis, P., Tešević, V.,& Topi, D.. (2022). LC-MS/MS characterization of phenolic compounds in the quince (Cydonia oblonga Mill.) and sweet cherry (Prunus avium L.) fruit juices. in Agriculture and Forestry
Biotechnical Faculty of the University of Montenegro., 68(2), 193-205.
https://doi.org/10.17707/AgricultForest.68.2.14

Llupa J, Gašić U, Brčeski I, Demertzis P, Tešević V, Topi D. LC-MS/MS characterization of phenolic compounds in the quince (Cydonia oblonga Mill.) and sweet cherry (Prunus avium L.) fruit juices. in Agriculture and Forestry. 2022;68(2):193-205.
doi:10.17707/AgricultForest.68.2.14 .

Llupa, Jonilda, Gašić, Uroš, Brčeski, Ilija, Demertzis, Panagiotis, Tešević, Vele, Topi, Dritan, "LC-MS/MS characterization of phenolic compounds in the quince (Cydonia oblonga Mill.) and sweet cherry (Prunus avium L.) fruit juices" in Agriculture and Forestry, 68, no. 2 (2022):193-205,
https://doi.org/10.17707/AgricultForest.68.2.14 . .

TY  - CONF
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Anđelković, Boban
AU  - Tešević, Vele
AU  - Milutinović, Milica
AU  - Skorić, Marijana
AU  - Savić, Jelena
PY  - 2022
UR  - https://www.plantslo.org/wp/wp-content/uploads/2022/09/PlantsInChangingEnvironment22AbstractBook.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5054
AB  - Essential oils (EOs) obtained from plants can frequently exhibit toxicity and repellency/deterrence against insect pests. Fennel (Foeniculum vulgare Mill.) and basil (Ocimum basilicum L.) EOs are used in pest management applications, but the most-discussed constraint regards restraining the usage of these natural insecticides in common agricultural practices is related to the volatility and limited persistence of EOs under field conditions. Due to high volatility, the efficacy of these substances falls short after EO application and requires frequent reapplication. There is a great interest in developing formulations that improve the handling of EOs, prevent the photodegradation of their active ingredients, and control the release of volatiles through micro/nanospheres or capsules in solid or liquid form. The polymer-based EO nanoformulations provide the stability and optimal effectiveness of EOs with an anti-insecticidal effect. One of the main advantages of solid formulations is that they can have a longer shelf life and be applied in solid form or even mixed with water and sprayed. We investigated the formation and characterization of inclusion complexes (ICs) of beta-cyclodextrin (β-CD) with two selected EOs, using the co-precipitation method. The main constituents of EOs determined by GC-MS analyses were trans-anethole (37.77%) and α-pinene (23.51%) in fennel EO, and linalool (32.6%) and methyl chavicol (23.2%) in basil EO. The interaction between β-CD and EO molecules was assessed through Fourier-transform infrared spectroscopy (FT-IR) and it was used to characterize the ICs by evaluating the changes recorded in the ICs compared to the free β-CD and individual EO. FT-IR spectra of inclusion complexes displayed peak shifts and variations of intensity when it compared to FT-IR spectra of EOs and free β-CD. β-cyclodextrin resulted as an effective host in encapsulating selected EOs. The potato plants sprayed with ICs were examined by scanning electron microscopy (SEM) and showed ICs with lamellate or rhomboid morphology. Bioassay with Colorado potato beetle fed with potato plants covered with ICs showed that complexation with β-CD reduced insects’ growth (body mass of IV instar larvae where 119.84±1.48 mg and 120.79±7.42 mg for IC with fennel and basil EO respectively in comparison to body mass of control larvae 138.97±2.01mg) and affected digestive proteolytic activity.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth
SP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5054
ER  - 

@conference{
author = "Devrnja, Nina and Šajkunić, Sanja and Anđelković, Boban and Tešević, Vele and Milutinović, Milica and Skorić, Marijana and Savić, Jelena",
year = "2022",
abstract = "Essential oils (EOs) obtained from plants can frequently exhibit toxicity and repellency/deterrence against insect pests. Fennel (Foeniculum vulgare Mill.) and basil (Ocimum basilicum L.) EOs are used in pest management applications, but the most-discussed constraint regards restraining the usage of these natural insecticides in common agricultural practices is related to the volatility and limited persistence of EOs under field conditions. Due to high volatility, the efficacy of these substances falls short after EO application and requires frequent reapplication. There is a great interest in developing formulations that improve the handling of EOs, prevent the photodegradation of their active ingredients, and control the release of volatiles through micro/nanospheres or capsules in solid or liquid form. The polymer-based EO nanoformulations provide the stability and optimal effectiveness of EOs with an anti-insecticidal effect. One of the main advantages of solid formulations is that they can have a longer shelf life and be applied in solid form or even mixed with water and sprayed. We investigated the formation and characterization of inclusion complexes (ICs) of beta-cyclodextrin (β-CD) with two selected EOs, using the co-precipitation method. The main constituents of EOs determined by GC-MS analyses were trans-anethole (37.77%) and α-pinene (23.51%) in fennel EO, and linalool (32.6%) and methyl chavicol (23.2%) in basil EO. The interaction between β-CD and EO molecules was assessed through Fourier-transform infrared spectroscopy (FT-IR) and it was used to characterize the ICs by evaluating the changes recorded in the ICs compared to the free β-CD and individual EO. FT-IR spectra of inclusion complexes displayed peak shifts and variations of intensity when it compared to FT-IR spectra of EOs and free β-CD. β-cyclodextrin resulted as an effective host in encapsulating selected EOs. The potato plants sprayed with ICs were examined by scanning electron microscopy (SEM) and showed ICs with lamellate or rhomboid morphology. Bioassay with Colorado potato beetle fed with potato plants covered with ICs showed that complexation with β-CD reduced insects’ growth (body mass of IV instar larvae where 119.84±1.48 mg and 120.79±7.42 mg for IC with fennel and basil EO respectively in comparison to body mass of control larvae 138.97±2.01mg) and affected digestive proteolytic activity.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth",
pages = "7",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5054"
}

Devrnja, N., Šajkunić, S., Anđelković, B., Tešević, V., Milutinović, M., Skorić, M.,& Savić, J.. (2022). Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Ljubljana: Slovenian Society of Plant Biology., 7.
https://hdl.handle.net/21.15107/rcub_ibiss_5054

Devrnja N, Šajkunić S, Anđelković B, Tešević V, Milutinović M, Skorić M, Savić J. Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:7.
https://hdl.handle.net/21.15107/rcub_ibiss_5054 .

Devrnja, Nina, Šajkunić, Sanja, Anđelković, Boban, Tešević, Vele, Milutinović, Milica, Skorić, Marijana, Savić, Jelena, "Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth" in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):7,
https://hdl.handle.net/21.15107/rcub_ibiss_5054 .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Stanković-Jeremić, Jovana
AU  - Maksimović, Vuk
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - internal-pdf://Stupar et al. - 2021 - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf St.pdf
UR  - https://www.mdpi.com/2223-7747/10/1/172
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4147
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI AG
T2  - Plants
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
SP  - 172
ER  - 

@article{
author = "Stupar, Sofija and Dragićević, Milan and Tešević, Vele and Stanković-Jeremić, Jovana and Maksimović, Vuk and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI AG",
journal = "Plants",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172",
pages = "172"
}

Stupar, S., Dragićević, M., Tešević, V., Stanković-Jeremić, J., Maksimović, V., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants
MDPI AG., 10(1), 172.
https://doi.org/10.3390/plants10010172

Stupar S, Dragićević M, Tešević V, Stanković-Jeremić J, Maksimović V, Ćosić T, Devrnja N, Tubić L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants. 2021;10(1):172.
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragićević, Milan, Tešević, Vele, Stanković-Jeremić, Jovana, Maksimović, Vuk, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants, 10, no. 1 (2021):172,
https://doi.org/10.3390/plants10010172 . .

TY  - JOUR
AU  - Cagnini, Caroline Zanella
AU  - Dias, Angélica Barbosa
AU  - Vilas Boas, Mariana Rodrigues
AU  - Batista, Fabiana Pacheco Reis
AU  - Faria, Maria Graciela Iecher
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Tešević, Vele
AU  - Ferreira, Ederlan de Souza
AU  - Colauto, Nelson Barros
AU  - Linde, Giani Andrea
AU  - Gazim, Zilda Cristiani
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4742
AB  - Bioactive compounds extracted from plants such as antimicrobials have attracted the attention of consumers and the food industry. This study aimed to determine the antimicrobial activity and chemical composition of Annona muricata leaf oleoresin obtained by supercritical CO2 extraction. The oleoresin was obtained by supercritical CO2 extraction and the chemical identification by gas chromatography coupled to mass spectrometry. Antimicrobial activity was evaluated by broth microdilution method against 14 foodborne fungi and bacteria. The oleoresin major chemical class was phytosterols (22.7%) and the major compounds were γ-sitosterol (15.7%), α-tocopherol (13.7%), phytol (13.1%), and hexadecanoic acid (11.5%). Minimum inhibitory concentration against bacteria ranged from 0.0025 to 0.010 mg mL-1. The oleoresin had high bactericidal activity against all bacteria, mainly Enterobacter cloacae and Pseudomonas aeruginosa with 0.005 mg mL-1 minimum bactericidal concentration. However, it had low fungicidal activity. The leaf oleoresin of A. muricata has promising applications in food, cosmetic, and pharmaceutical industries.
T2  - Natural product research
T1  - Antimicrobial activity of Annona muricata leaf oleoresin.
DO  - 10.1080/14786419.2021.2011270
ER  - 

@article{
author = "Cagnini, Caroline Zanella and Dias, Angélica Barbosa and Vilas Boas, Mariana Rodrigues and Batista, Fabiana Pacheco Reis and Faria, Maria Graciela Iecher and Glamočlija, Jasmina and Soković, Marina and Tešević, Vele and Ferreira, Ederlan de Souza and Colauto, Nelson Barros and Linde, Giani Andrea and Gazim, Zilda Cristiani",
year = "2021",
abstract = "Bioactive compounds extracted from plants such as antimicrobials have attracted the attention of consumers and the food industry. This study aimed to determine the antimicrobial activity and chemical composition of Annona muricata leaf oleoresin obtained by supercritical CO2 extraction. The oleoresin was obtained by supercritical CO2 extraction and the chemical identification by gas chromatography coupled to mass spectrometry. Antimicrobial activity was evaluated by broth microdilution method against 14 foodborne fungi and bacteria. The oleoresin major chemical class was phytosterols (22.7%) and the major compounds were γ-sitosterol (15.7%), α-tocopherol (13.7%), phytol (13.1%), and hexadecanoic acid (11.5%). Minimum inhibitory concentration against bacteria ranged from 0.0025 to 0.010 mg mL-1. The oleoresin had high bactericidal activity against all bacteria, mainly Enterobacter cloacae and Pseudomonas aeruginosa with 0.005 mg mL-1 minimum bactericidal concentration. However, it had low fungicidal activity. The leaf oleoresin of A. muricata has promising applications in food, cosmetic, and pharmaceutical industries.",
journal = "Natural product research",
title = "Antimicrobial activity of Annona muricata leaf oleoresin.",
doi = "10.1080/14786419.2021.2011270"
}

Cagnini, C. Z., Dias, A. B., Vilas Boas, M. R., Batista, F. P. R., Faria, M. G. I., Glamočlija, J., Soković, M., Tešević, V., Ferreira, E. d. S., Colauto, N. B., Linde, G. A.,& Gazim, Z. C.. (2021). Antimicrobial activity of Annona muricata leaf oleoresin.. in Natural product research.
https://doi.org/10.1080/14786419.2021.2011270

Cagnini CZ, Dias AB, Vilas Boas MR, Batista FPR, Faria MGI, Glamočlija J, Soković M, Tešević V, Ferreira EDS, Colauto NB, Linde GA, Gazim ZC. Antimicrobial activity of Annona muricata leaf oleoresin.. in Natural product research. 2021;.
doi:10.1080/14786419.2021.2011270 .

Cagnini, Caroline Zanella, Dias, Angélica Barbosa, Vilas Boas, Mariana Rodrigues, Batista, Fabiana Pacheco Reis, Faria, Maria Graciela Iecher, Glamočlija, Jasmina, Soković, Marina, Tešević, Vele, Ferreira, Ederlan de Souza, Colauto, Nelson Barros, Linde, Giani Andrea, Gazim, Zilda Cristiani, "Antimicrobial activity of Annona muricata leaf oleoresin." in Natural product research (2021),
https://doi.org/10.1080/14786419.2021.2011270 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Krstić Milošević, Dijana
AU  - Janošević, Dušica
AU  - Tešević, Vele
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
AU  - Ćalić, Dušica
PY  - 2021
UR  - http://link.springer.com/10.1007/s00709-020-01588-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4038
AB  - In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.
PB  - Springer
T2  - Protoplasma
T1  - In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents
IS  - 3
VL  - 258
DO  - 10.1007/s00709-020-01588-9
SP  - 587
EP  - 599
ER  - 

@article{
author = "Devrnja, Nina and Krstić Milošević, Dijana and Janošević, Dušica and Tešević, Vele and Vinterhalter, Branka and Savić, Jelena and Ćalić, Dušica",
year = "2021",
abstract = "In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.",
publisher = "Springer",
journal = "Protoplasma",
title = "In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents",
number = "3",
volume = "258",
doi = "10.1007/s00709-020-01588-9",
pages = "587-599"
}

Devrnja, N., Krstić Milošević, D., Janošević, D., Tešević, V., Vinterhalter, B., Savić, J.,& Ćalić, D.. (2021). In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents. in Protoplasma
Springer., 258(3), 587-599.
https://doi.org/10.1007/s00709-020-01588-9

Devrnja N, Krstić Milošević D, Janošević D, Tešević V, Vinterhalter B, Savić J, Ćalić D. In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents. in Protoplasma. 2021;258(3):587-599.
doi:10.1007/s00709-020-01588-9 .

Devrnja, Nina, Krstić Milošević, Dijana, Janošević, Dušica, Tešević, Vele, Vinterhalter, Branka, Savić, Jelena, Ćalić, Dušica, "In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents" in Protoplasma, 258, no. 3 (2021):587-599,
https://doi.org/10.1007/s00709-020-01588-9 . .

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Tešević, Vele
AU  - Đorđević, Iris
AU  - Todosijević, Marina
AU  - Mitić, Zorica
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3710
AB  - The variability of n-alkanes in the needle cuticular wax of Pinus heldreichii and P. peuce in two natural populations from the Scardo-Pindic mountains was investigated for the first time. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) analyses of two-year-old needles were performed us-ing an Agilent 7890A GC equipped with an inert 5975C XL EI/CI mass spectrometer detector (MSD) and a flame ionization detector (FID) connected by a capillary flow technology 2-way splitter with make-up. An HP-5MS capillary column was used. n-Alkanes ranged from C19 to C33. In P. heldreichii the most abundant were C23, C25 and C27 (16.3, 15.6 and 12.8 % on average, respectively), while in P. peuce they were C29, C25, and C27 (16.5, 15.3 and 13.5 % on average, resp.). Mid-chain and long-chain n-alkanes prevailed in both species. Principle component analysis (PCA) and Cluster analyses of five and six n-alkanes, respectively, showed divergence of the Scardo-Pindic populations from the Dinaric ones.
PB  - Macedonian Journal of Chemistry and Chemical Engineering
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains
IS  - 1
VL  - 39
DO  - 10.20450/mjcce.2020.1951
SP  - 41
EP  - 48
ER  - 

@article{
author = "Nikolić, Biljana and Tešević, Vele and Đorđević, Iris and Todosijević, Marina and Mitić, Zorica and Bojović, Srđan and Marin, Petar D.",
year = "2020",
abstract = "The variability of n-alkanes in the needle cuticular wax of Pinus heldreichii and P. peuce in two natural populations from the Scardo-Pindic mountains was investigated for the first time. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) analyses of two-year-old needles were performed us-ing an Agilent 7890A GC equipped with an inert 5975C XL EI/CI mass spectrometer detector (MSD) and a flame ionization detector (FID) connected by a capillary flow technology 2-way splitter with make-up. An HP-5MS capillary column was used. n-Alkanes ranged from C19 to C33. In P. heldreichii the most abundant were C23, C25 and C27 (16.3, 15.6 and 12.8 % on average, respectively), while in P. peuce they were C29, C25, and C27 (16.5, 15.3 and 13.5 % on average, resp.). Mid-chain and long-chain n-alkanes prevailed in both species. Principle component analysis (PCA) and Cluster analyses of five and six n-alkanes, respectively, showed divergence of the Scardo-Pindic populations from the Dinaric ones.",
publisher = "Macedonian Journal of Chemistry and Chemical Engineering",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains",
number = "1",
volume = "39",
doi = "10.20450/mjcce.2020.1951",
pages = "41-48"
}

Nikolić, B., Tešević, V., Đorđević, I., Todosijević, M., Mitić, Z., Bojović, S.,& Marin, P. D.. (2020). Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains. in Macedonian Journal of Chemistry and Chemical Engineering
Macedonian Journal of Chemistry and Chemical Engineering., 39(1), 41-48.
https://doi.org/10.20450/mjcce.2020.1951

Nikolić B, Tešević V, Đorđević I, Todosijević M, Mitić Z, Bojović S, Marin PD. Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains. in Macedonian Journal of Chemistry and Chemical Engineering. 2020;39(1):41-48.
doi:10.20450/mjcce.2020.1951 .

Nikolić, Biljana, Tešević, Vele, Đorđević, Iris, Todosijević, Marina, Mitić, Zorica, Bojović, Srđan, Marin, Petar D., "Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains" in Macedonian Journal of Chemistry and Chemical Engineering, 39, no. 1 (2020):41-48,
https://doi.org/10.20450/mjcce.2020.1951 . .

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Ljujić, Jovana
AU  - Bojović, Srđan
AU  - Mitić, Zorica
AU  - Rajčević, Nemanja
AU  - Tešević, Vele
AU  - Marin, Petar
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642000038N
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4040
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5348
AB  - The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were ?-pinene (29.5 %), ?-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, ?-phellandrene, ?-3-carene, p-cymene, ?-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (?-pinene, ?-pinene, myrcene, ?-3-carene, ?-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Headspace volatiles isolated from twigs of Picea omorika from Serbia
IS  - 3
VL  - 72
DO  - 10.2298/ABS200511038N
SP  - 445
EP  - 452
ER  - 

@article{
author = "Nikolić, Biljana and Ljujić, Jovana and Bojović, Srđan and Mitić, Zorica and Rajčević, Nemanja and Tešević, Vele and Marin, Petar",
year = "2020",
abstract = "The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were ?-pinene (29.5 %), ?-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, ?-phellandrene, ?-3-carene, p-cymene, ?-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (?-pinene, ?-pinene, myrcene, ?-3-carene, ?-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Headspace volatiles isolated from twigs of Picea omorika from Serbia",
number = "3",
volume = "72",
doi = "10.2298/ABS200511038N",
pages = "445-452"
}

Nikolić, B., Ljujić, J., Bojović, S., Mitić, Z., Rajčević, N., Tešević, V.,& Marin, P.. (2020). Headspace volatiles isolated from twigs of Picea omorika from Serbia. in Archives of Biological Sciences
Serbian Biological Society., 72(3), 445-452.
https://doi.org/10.2298/ABS200511038N

Nikolić B, Ljujić J, Bojović S, Mitić Z, Rajčević N, Tešević V, Marin P. Headspace volatiles isolated from twigs of Picea omorika from Serbia. in Archives of Biological Sciences. 2020;72(3):445-452.
doi:10.2298/ABS200511038N .

Nikolić, Biljana, Ljujić, Jovana, Bojović, Srđan, Mitić, Zorica, Rajčević, Nemanja, Tešević, Vele, Marin, Petar, "Headspace volatiles isolated from twigs of Picea omorika from Serbia" in Archives of Biological Sciences, 72, no. 3 (2020):445-452,
https://doi.org/10.2298/ABS200511038N . .

TY  - JOUR
AU  - Nikolić, Biljana M.
AU  - Đorđević, Iris
AU  - Todosijević, Marina
AU  - Mitić, Zorica
AU  - Marković, Milena
AU  - Stanković, Jovana M.
AU  - Bojović, Srđan
AU  - Tešević, Vele
AU  - Marin, Petar D.
PY  - 2020
UR  - https://www.tandfonline.com/doi/abs/10.1080/11263504.2020.1857867
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4080
AB  - The content of nonacosan-10-ol and n-alkanes in needle waxes of 12 Pinus taxa (P. halepensis, P. heldreichii, P. mugo, P. nigra ssp. nigra, P. nigra ssp. dalmatica, P. peuce, P. pinaster, P. pinea, P. ponderosa, P. strobus, P. sylvestris and P. wallichiana) were examined. In eight Pinus taxa (except of P. halepensis, P. pinaster, P. heldreichii and P. peuce) nonacosan-10-ol contents were examined for the first time. In all examined pines C29, C27 or C25 were the main n-alkane compounds. C range was mainly 18-–33. In six Pinus taxa (P. mugo, P. nigra ssp. dalmatica, P. sylvestris, P. pinea, P. strobus and P. wallichiana) carbon preference index and average chain length values were examined for the first time. In the plane of Axis 1 (PCA), P. strobus, P. mugo and P. wallichiana were strongly separated from P. heldreichii and P. pinea. In the plane of Axis 2, P. peuce, P. strobus and P. wallichiana as well as P. heldreichii and P. pinea diverged from other examined species. In DA, P. heldreichii, P. strobus, P. peuce and P. wallichiana diversed. CA divided sections Pinus and Pinaster from the section Strobi.
PB  - Taylor and Francis Ltd.
T2  - Plant Biosystems
T1  - Diversity of nonacosan-10-ol and n-alkanes among 12 Pinus taxa
DO  - 10.1080/11263504.2020.1857867
ER  - 

@article{
author = "Nikolić, Biljana M. and Đorđević, Iris and Todosijević, Marina and Mitić, Zorica and Marković, Milena and Stanković, Jovana M. and Bojović, Srđan and Tešević, Vele and Marin, Petar D.",
year = "2020",
abstract = "The content of nonacosan-10-ol and n-alkanes in needle waxes of 12 Pinus taxa (P. halepensis, P. heldreichii, P. mugo, P. nigra ssp. nigra, P. nigra ssp. dalmatica, P. peuce, P. pinaster, P. pinea, P. ponderosa, P. strobus, P. sylvestris and P. wallichiana) were examined. In eight Pinus taxa (except of P. halepensis, P. pinaster, P. heldreichii and P. peuce) nonacosan-10-ol contents were examined for the first time. In all examined pines C29, C27 or C25 were the main n-alkane compounds. C range was mainly 18-–33. In six Pinus taxa (P. mugo, P. nigra ssp. dalmatica, P. sylvestris, P. pinea, P. strobus and P. wallichiana) carbon preference index and average chain length values were examined for the first time. In the plane of Axis 1 (PCA), P. strobus, P. mugo and P. wallichiana were strongly separated from P. heldreichii and P. pinea. In the plane of Axis 2, P. peuce, P. strobus and P. wallichiana as well as P. heldreichii and P. pinea diverged from other examined species. In DA, P. heldreichii, P. strobus, P. peuce and P. wallichiana diversed. CA divided sections Pinus and Pinaster from the section Strobi.",
publisher = "Taylor and Francis Ltd.",
journal = "Plant Biosystems",
title = "Diversity of nonacosan-10-ol and n-alkanes among 12 Pinus taxa",
doi = "10.1080/11263504.2020.1857867"
}

Nikolić, B. M., Đorđević, I., Todosijević, M., Mitić, Z., Marković, M., Stanković, J. M., Bojović, S., Tešević, V.,& Marin, P. D.. (2020). Diversity of nonacosan-10-ol and n-alkanes among 12 Pinus taxa. in Plant Biosystems
Taylor and Francis Ltd...
https://doi.org/10.1080/11263504.2020.1857867

Nikolić BM, Đorđević I, Todosijević M, Mitić Z, Marković M, Stanković JM, Bojović S, Tešević V, Marin PD. Diversity of nonacosan-10-ol and n-alkanes among 12 Pinus taxa. in Plant Biosystems. 2020;.
doi:10.1080/11263504.2020.1857867 .

Nikolić, Biljana M., Đorđević, Iris, Todosijević, Marina, Mitić, Zorica, Marković, Milena, Stanković, Jovana M., Bojović, Srđan, Tešević, Vele, Marin, Petar D., "Diversity of nonacosan-10-ol and n-alkanes among 12 Pinus taxa" in Plant Biosystems (2020),
https://doi.org/10.1080/11263504.2020.1857867 . .

TY  - JOUR
AU  - Vujić, Bojan
AU  - Vidaković, Vera
AU  - Jadranin, Milka
AU  - Novaković, Irena
AU  - Trifunović, Snežana
AU  - Tešević, Vele
AU  - Mandić, Boris
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3620
AB  - Helichrysum plicatum DC. is widely used in folk medicine in treating a variety of health disorders. The aim of this study was to examine the influence of different extraction solvents on the chemical composition, antioxidant potential, and antimicrobial activities of H. plicatum. Aerial parts were separately extracted with ethanol, dichloromethane, and sunflower oil. The oil extract (OE) was re-extracted with acetonitrile. A total of 142 compounds were tentatively identified in ethanolic (EE), dichloromethane (DCME), and acetonitrile (ACNE) extracts using HPLC-DAD/ESI-ToF-MS. The dominant compound class in all extracts were α-pyrones, alongside flavonoids in EE, terpenoids in DCME and ACNE, and phloroglucinols in DCME. The antioxidant potential of the extracts was assessed by the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay. EE and DCME possessed the most potent radical scavenging capacity. Antimicrobial activity was investigated on eight bacterial, two yeast, and one fungal species. All extracts exhibited high antifungal and notable antibacterial activities compared to control substances, with DCME being the most potent. DCME exhibited stronger antimicrobial activity against P. aeruginosa than the standard chloramphenicol.
PB  - Basel : MDPI
T2  - Plants
T1  - Composition, Antioxidant Potential, and Antimicrobial Activity of Helichrysum plicatum DC. Various Extracts
IS  - 3
VL  - 9
DO  - 10.3390/plants9030337
SP  - 337
ER  - 

@article{
author = "Vujić, Bojan and Vidaković, Vera and Jadranin, Milka and Novaković, Irena and Trifunović, Snežana and Tešević, Vele and Mandić, Boris",
year = "2020",
abstract = "Helichrysum plicatum DC. is widely used in folk medicine in treating a variety of health disorders. The aim of this study was to examine the influence of different extraction solvents on the chemical composition, antioxidant potential, and antimicrobial activities of H. plicatum. Aerial parts were separately extracted with ethanol, dichloromethane, and sunflower oil. The oil extract (OE) was re-extracted with acetonitrile. A total of 142 compounds were tentatively identified in ethanolic (EE), dichloromethane (DCME), and acetonitrile (ACNE) extracts using HPLC-DAD/ESI-ToF-MS. The dominant compound class in all extracts were α-pyrones, alongside flavonoids in EE, terpenoids in DCME and ACNE, and phloroglucinols in DCME. The antioxidant potential of the extracts was assessed by the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay. EE and DCME possessed the most potent radical scavenging capacity. Antimicrobial activity was investigated on eight bacterial, two yeast, and one fungal species. All extracts exhibited high antifungal and notable antibacterial activities compared to control substances, with DCME being the most potent. DCME exhibited stronger antimicrobial activity against P. aeruginosa than the standard chloramphenicol.",
publisher = "Basel : MDPI",
journal = "Plants",
title = "Composition, Antioxidant Potential, and Antimicrobial Activity of Helichrysum plicatum DC. Various Extracts",
number = "3",
volume = "9",
doi = "10.3390/plants9030337",
pages = "337"
}

Vujić, B., Vidaković, V., Jadranin, M., Novaković, I., Trifunović, S., Tešević, V.,& Mandić, B.. (2020). Composition, Antioxidant Potential, and Antimicrobial Activity of Helichrysum plicatum DC. Various Extracts. in Plants
Basel : MDPI., 9(3), 337.
https://doi.org/10.3390/plants9030337

Vujić B, Vidaković V, Jadranin M, Novaković I, Trifunović S, Tešević V, Mandić B. Composition, Antioxidant Potential, and Antimicrobial Activity of Helichrysum plicatum DC. Various Extracts. in Plants. 2020;9(3):337.
doi:10.3390/plants9030337 .

Vujić, Bojan, Vidaković, Vera, Jadranin, Milka, Novaković, Irena, Trifunović, Snežana, Tešević, Vele, Mandić, Boris, "Composition, Antioxidant Potential, and Antimicrobial Activity of Helichrysum plicatum DC. Various Extracts" in Plants, 9, no. 3 (2020):337,
https://doi.org/10.3390/plants9030337 . .

TY  - CONF
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4368
AB  - In beetles, secretions are products of different exocrine glands, of which particular 
emphasis is on pygidial glands, which are common for the suborder Adephaga. 
Antimicrobial activity of the pygidial gland secretions of six selected ground beetle 
species was observed against human pathogens. A microdilution method was applied 
in order to determine minimum inhibitory concentrations (MICs), minimum bactericidal 
concentrations (MBCs) and minimum fungicidal concentrations (MFCs). We tested 
16 laboratory and clinical strains of human pathogens (eight bacterial gram-positive 
and gram-negative species, and eight fungal species). The secretions of tested ground 
beetles have a certain level of antimicrobial activity, which differed between species, 
which is caused by the difference in chemical composition of the mixtures. The highest 
antibacterial effect was observed after treatment of most pathogens with secretion of 
Laemostenus punctatus and Carabus ullrichii. The highest resistance of tested pathogenic 
strains was noticed after treatment of the secretion of Calosoma sycophanta, which 
achieved antibacterial effect against E. coli only. The secretions of L. punctatus and C. 
sycophanta each showed a strong antifungal effect compared to positive controls. 
Inhibition of the proliferation of human tumor cell lines and porcine non-tumor 
cells by the secretion of adults of four ground beetle species was observed as well. 
The sulphorhodamine B (SRB) assay was applied to establish GI50 values of the tested 
secretions. All secretions have shown certain antiproliferative effect on the tested 
cell lines, but statistically insignificant in most cases, which confirms the absence of 
cytotoxicity on the non-tumor cell line. Special emphasis is put on the secretion of L. 
punctatus, which reached approximately 50% inhibition of cell proliferation (GI50 value) 
of MCF7 (breast adenocarcinoma) cell line, and showed a potential antitumour activity. 
The antimicrobial, antitumour and antiproliferative potential of the secretions of ground 
beetles was demonstrated for the first time in this study.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade
C3  - The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
T1  - The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4368
ER  - 

@conference{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Tešević, Vele and Vujisić, Ljubodrag and Vesović, Nikola and Ćurčić, Srećko",
year = "2019",
abstract = "In beetles, secretions are products of different exocrine glands, of which particular 
emphasis is on pygidial glands, which are common for the suborder Adephaga. 
Antimicrobial activity of the pygidial gland secretions of six selected ground beetle 
species was observed against human pathogens. A microdilution method was applied 
in order to determine minimum inhibitory concentrations (MICs), minimum bactericidal 
concentrations (MBCs) and minimum fungicidal concentrations (MFCs). We tested 
16 laboratory and clinical strains of human pathogens (eight bacterial gram-positive 
and gram-negative species, and eight fungal species). The secretions of tested ground 
beetles have a certain level of antimicrobial activity, which differed between species, 
which is caused by the difference in chemical composition of the mixtures. The highest 
antibacterial effect was observed after treatment of most pathogens with secretion of 
Laemostenus punctatus and Carabus ullrichii. The highest resistance of tested pathogenic 
strains was noticed after treatment of the secretion of Calosoma sycophanta, which 
achieved antibacterial effect against E. coli only. The secretions of L. punctatus and C. 
sycophanta each showed a strong antifungal effect compared to positive controls. 
Inhibition of the proliferation of human tumor cell lines and porcine non-tumor 
cells by the secretion of adults of four ground beetle species was observed as well. 
The sulphorhodamine B (SRB) assay was applied to establish GI50 values of the tested 
secretions. All secretions have shown certain antiproliferative effect on the tested 
cell lines, but statistically insignificant in most cases, which confirms the absence of 
cytotoxicity on the non-tumor cell line. Special emphasis is put on the secretion of L. 
punctatus, which reached approximately 50% inhibition of cell proliferation (GI50 value) 
of MCF7 (breast adenocarcinoma) cell line, and showed a potential antitumour activity. 
The antimicrobial, antitumour and antiproliferative potential of the secretions of ground 
beetles was demonstrated for the first time in this study.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia",
title = "The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4368"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Tešević, V., Vujisić, L., Vesović, N.,& Ćurčić, S.. (2019). The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade., 43.
https://hdl.handle.net/21.15107/rcub_ibiss_4368

Nenadić M, Soković M, Glamočlija J, Ćirić A, Tešević V, Vujisić L, Vesović N, Ćurčić S. The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia. 2019;:43.
https://hdl.handle.net/21.15107/rcub_ibiss_4368 .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Tešević, Vele, Vujisić, Ljubodrag, Vesović, Nikola, Ćurčić, Srećko, "The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products" in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia (2019):43,
https://hdl.handle.net/21.15107/rcub_ibiss_4368 .

TY  - JOUR
AU  - Stanković, Jovana
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Zdunić, Gordana
AU  - Dajić-Stevanović, Zora
AU  - Gođevac, Dejan
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3613
AB  - This study was performed to determine the main antibacterial com­po­unds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Aster­aceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was deter­mined by 1D- and 2D-NMR and GC–MS techniques. Antibacterial acti­vity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further inves­tigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and iso­geranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, res­pect­ively, at 1/2 MIC concentrations.
T2  - Journal of the Serbian Chemical Society
T1  - HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil
IS  - 12
VL  - 84
DO  - 10.2298/jsc190513106s
SP  - 1355
EP  - 1365
ER  - 

@article{
author = "Stanković, Jovana and Novaković, Miroslav and Tešević, Vele and Ćirić, Ana and Soković, Marina and Zdunić, Gordana and Dajić-Stevanović, Zora and Gođevac, Dejan",
year = "2019",
abstract = "This study was performed to determine the main antibacterial com­po­unds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Aster­aceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was deter­mined by 1D- and 2D-NMR and GC–MS techniques. Antibacterial acti­vity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further inves­tigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and iso­geranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, res­pect­ively, at 1/2 MIC concentrations.",
journal = "Journal of the Serbian Chemical Society",
title = "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil",
number = "12",
volume = "84",
doi = "10.2298/jsc190513106s",
pages = "1355-1365"
}

Stanković, J., Novaković, M., Tešević, V., Ćirić, A., Soković, M., Zdunić, G., Dajić-Stevanović, Z.,& Gođevac, D.. (2019). HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in Journal of the Serbian Chemical Society, 84(12), 1355-1365.
https://doi.org/10.2298/jsc190513106s

Stanković J, Novaković M, Tešević V, Ćirić A, Soković M, Zdunić G, Dajić-Stevanović Z, Gođevac D. HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in Journal of the Serbian Chemical Society. 2019;84(12):1355-1365.
doi:10.2298/jsc190513106s .

Stanković, Jovana, Novaković, Miroslav, Tešević, Vele, Ćirić, Ana, Soković, Marina, Zdunić, Gordana, Dajić-Stevanović, Zora, Gođevac, Dejan, "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil" in Journal of the Serbian Chemical Society, 84, no. 12 (2019):1355-1365,
https://doi.org/10.2298/jsc190513106s . .

TY  - JOUR
AU  - Stanković, Jovana
AU  - Gođevac, Dejan
AU  - Tešević, Vele
AU  - Dajić-Stevanović, Zora
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Novaković, Miroslav
PY  - 2019
UR  - http://pubs.acs.org/doi/10.1021/acs.jnatprod.8b00970
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3397
AB  - A new flavonoid glucoside derivative, patuletin 3 -O-(2- O-feruloyl)-β-d-glucuronopyranosyl-(1→2)-β-d-glucopyranoside, named atriplexin IV (1), and three new triterpenoid saponin derivatives, two sulfonylated, β-d-glucopyranosyl-3 -O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside-30-alolean-12-en-28-oate (2), named atriplexogenin I, β-d-glucopyranosyl-3- O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside)-30-hydroxyolean-12-en-28-oate (3), named atriplexogenin II, and β-d-glucopyranosyl-3 -O-(β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-(1→2)-α-l-arabinopyranoside)-30-alolean-12-en-28-oate (4), named atriplexogenin III, were isolated by silica gel column and semipreparative HPLC chromatography from the n-butanol extract of the salt marsh plant Atriplex tatarica. In addition, two known secondary metabolites, patuletin3 -O-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (5) and patuletin 3 -O-5‴- O-feruloyl-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (6), were isolated for the first time from A. tatarica. The structures of the isolated compounds were elucidated by 1D and 2D NMR, HRESIMS, IR, and UV data. Antibacterial activity by the microdilution method and antibiofilm activity against P. aeruginosa were assessed. Compound 5 possesses significant antibacterial activity, while the most potent antibiofilm agent is compound 2.
PB  - American Chemical Society and American Society of Pharmacognosy
T2  - Journal of Natural Products
T1  - Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.
IS  - 6
VL  - 82
DO  - 10.1021/acs.jnatprod.8b00970
SP  - 1487
EP  - 1495
ER  - 

@article{
author = "Stanković, Jovana and Gođevac, Dejan and Tešević, Vele and Dajić-Stevanović, Zora and Ćirić, Ana and Soković, Marina and Novaković, Miroslav",
year = "2019",
abstract = "A new flavonoid glucoside derivative, patuletin 3 -O-(2- O-feruloyl)-β-d-glucuronopyranosyl-(1→2)-β-d-glucopyranoside, named atriplexin IV (1), and three new triterpenoid saponin derivatives, two sulfonylated, β-d-glucopyranosyl-3 -O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside-30-alolean-12-en-28-oate (2), named atriplexogenin I, β-d-glucopyranosyl-3- O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside)-30-hydroxyolean-12-en-28-oate (3), named atriplexogenin II, and β-d-glucopyranosyl-3 -O-(β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-(1→2)-α-l-arabinopyranoside)-30-alolean-12-en-28-oate (4), named atriplexogenin III, were isolated by silica gel column and semipreparative HPLC chromatography from the n-butanol extract of the salt marsh plant Atriplex tatarica. In addition, two known secondary metabolites, patuletin3 -O-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (5) and patuletin 3 -O-5‴- O-feruloyl-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (6), were isolated for the first time from A. tatarica. The structures of the isolated compounds were elucidated by 1D and 2D NMR, HRESIMS, IR, and UV data. Antibacterial activity by the microdilution method and antibiofilm activity against P. aeruginosa were assessed. Compound 5 possesses significant antibacterial activity, while the most potent antibiofilm agent is compound 2.",
publisher = "American Chemical Society and American Society of Pharmacognosy",
journal = "Journal of Natural Products",
title = "Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.",
number = "6",
volume = "82",
doi = "10.1021/acs.jnatprod.8b00970",
pages = "1487-1495"
}

Stanković, J., Gođevac, D., Tešević, V., Dajić-Stevanović, Z., Ćirić, A., Soković, M.,& Novaković, M.. (2019). Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.. in Journal of Natural Products
American Chemical Society and American Society of Pharmacognosy., 82(6), 1487-1495.
https://doi.org/10.1021/acs.jnatprod.8b00970

Stanković J, Gođevac D, Tešević V, Dajić-Stevanović Z, Ćirić A, Soković M, Novaković M. Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.. in Journal of Natural Products. 2019;82(6):1487-1495.
doi:10.1021/acs.jnatprod.8b00970 .

Stanković, Jovana, Gođevac, Dejan, Tešević, Vele, Dajić-Stevanović, Zora, Ćirić, Ana, Soković, Marina, Novaković, Miroslav, "Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa." in Journal of Natural Products, 82, no. 6 (2019):1487-1495,
https://doi.org/10.1021/acs.jnatprod.8b00970 . .

TY  - JOUR
AU  - Bukvički, Danka
AU  - Giweli, Abdulhmid
AU  - Stojković, Dejan
AU  - Vujisić, Ljubodrag
AU  - Tešević, Vele
AU  - Nikolić, Miloš
AU  - Soković, Marina
AU  - Marin, Petar D
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3032
UR  - http://www.journalofdairyscience.org/article/S0022-0302(18)30148-6/fulltext
AB  - The essential oil of Thymus algeriensis was analyzed as a potential preservative in soft cheese. We developed a novel method to test the preserving properties of essential oil in soft cheese. Contamination incidence of Penicillium aurantiogriseum was absent after 30 d of storage at 4°C with 25 µL of essential oil added. The antimicrobial activity was tested against 8 bacteria and 8 fungi. Thymus algeriensis oil showed inhibitory activity against tested bacteria at 0.03 to 0.09 mg/mL, and bactericidal activity was achieved at 0.05 to 0.15 mg/mL. For antifungal activity, minimum inhibitory concentrations ranged between 0.01 and 0.04 mg/mL and minimum fungicidal concentrations between 0.01 and 0.04 mg/mL. Furthermore, the oil was also screened for antiradical activity using the 1,1-diphenyl-2-picrylhydrazyl assay. The results showed that the oil was active and achieved half-maximal inhibitory activity at 0.132 mg/mL. We used gas chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry to investigate the volatile compounds from the oil. Carvacrol was identified as the main compound in the oil, represented by 80.9% of the total constituents, followed by p-cymene (7.7%).
T2  - Journal of Dairy Science
T1  - Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative.
IS  - 5
VL  - 101
DO  - 10.3168/jds.2017-13714
SP  - 3859
EP  - 3865
ER  - 

@article{
author = "Bukvički, Danka and Giweli, Abdulhmid and Stojković, Dejan and Vujisić, Ljubodrag and Tešević, Vele and Nikolić, Miloš and Soković, Marina and Marin, Petar D",
year = "2018",
abstract = "The essential oil of Thymus algeriensis was analyzed as a potential preservative in soft cheese. We developed a novel method to test the preserving properties of essential oil in soft cheese. Contamination incidence of Penicillium aurantiogriseum was absent after 30 d of storage at 4°C with 25 µL of essential oil added. The antimicrobial activity was tested against 8 bacteria and 8 fungi. Thymus algeriensis oil showed inhibitory activity against tested bacteria at 0.03 to 0.09 mg/mL, and bactericidal activity was achieved at 0.05 to 0.15 mg/mL. For antifungal activity, minimum inhibitory concentrations ranged between 0.01 and 0.04 mg/mL and minimum fungicidal concentrations between 0.01 and 0.04 mg/mL. Furthermore, the oil was also screened for antiradical activity using the 1,1-diphenyl-2-picrylhydrazyl assay. The results showed that the oil was active and achieved half-maximal inhibitory activity at 0.132 mg/mL. We used gas chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry to investigate the volatile compounds from the oil. Carvacrol was identified as the main compound in the oil, represented by 80.9% of the total constituents, followed by p-cymene (7.7%).",
journal = "Journal of Dairy Science",
title = "Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative.",
number = "5",
volume = "101",
doi = "10.3168/jds.2017-13714",
pages = "3859-3865"
}

Bukvički, D., Giweli, A., Stojković, D., Vujisić, L., Tešević, V., Nikolić, M., Soković, M.,& Marin, P. D.. (2018). Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative.. in Journal of Dairy Science, 101(5), 3859-3865.
https://doi.org/10.3168/jds.2017-13714

Bukvički D, Giweli A, Stojković D, Vujisić L, Tešević V, Nikolić M, Soković M, Marin PD. Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative.. in Journal of Dairy Science. 2018;101(5):3859-3865.
doi:10.3168/jds.2017-13714 .

Bukvički, Danka, Giweli, Abdulhmid, Stojković, Dejan, Vujisić, Ljubodrag, Tešević, Vele, Nikolić, Miloš, Soković, Marina, Marin, Petar D, "Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative." in Journal of Dairy Science, 101, no. 5 (2018):3859-3865,
https://doi.org/10.3168/jds.2017-13714 . .

TY  - CONF
AU  - Stupar, Sofija
AU  - Tešević, Vele
AU  - Stanković, Jovana
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4329
AB  - When attacked, plants activate defense mechanisms including volatile emission. Besides having
a pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.
French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground parts
of plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysis
revealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.
The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)
as the most abundant compounds. In order to investigate the potential of marigold EO to induce
the defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesis
related PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EO
volatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,
and plants were then additionally injured by mechanically wounding leaves across the leaf surface.
After 24 h of wounding, another two leaves from each plant were collected and expression
of the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,
while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression of
all genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plants
exposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar to
that detected in untreated controls. These findings support the efforts for environmentally-safe
pest control in crops by using natural compounds.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil
SP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4329
ER  - 

@conference{
author = "Stupar, Sofija and Tešević, Vele and Stanković, Jovana and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2018",
abstract = "When attacked, plants activate defense mechanisms including volatile emission. Besides having
a pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.
French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground parts
of plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysis
revealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.
The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)
as the most abundant compounds. In order to investigate the potential of marigold EO to induce
the defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesis
related PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EO
volatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,
and plants were then additionally injured by mechanically wounding leaves across the leaf surface.
After 24 h of wounding, another two leaves from each plant were collected and expression
of the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,
while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression of
all genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plants
exposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar to
that detected in untreated controls. These findings support the efforts for environmentally-safe
pest control in crops by using natural compounds.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil",
pages = "58",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4329"
}

Stupar, S., Tešević, V., Stanković, J., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2018). Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 58.
https://hdl.handle.net/21.15107/rcub_ibiss_4329

Stupar S, Tešević V, Stanković J, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;:58.
https://hdl.handle.net/21.15107/rcub_ibiss_4329 .

Stupar, Sofija, Tešević, Vele, Stanković, Jovana, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018):58,
https://hdl.handle.net/21.15107/rcub_ibiss_4329 .

TY  - CONF
AU  - Devrnja, Nina
AU  - Janošević, Dušica
AU  - Tešević, Vele
AU  - Savić, Jelena
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
AU  - Ćalić, Dušica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4323
AB  - The morphology and structure of tansy glandular trichomes were characterized for the first
time using scanning electron microscopy and light microscopy, and their secretion was analyzed
by histochemical staining. Leaf indumentum of tansy was covered with non-glandular and glandular trichomes of biseriate type, distributed on both adaxial and abaxial surface. Both types of
trichomes were also distributed along the stem, but with lower density. Light microscopy showed
that biseriate glandular trichomes consisted of two broad basal cells embedded in the epidermis,
a short single-celled stalk, and a large round multicellular head. The histochemical analysis of the
glandular secretion, performed through several staining methods, revealed the complex nature of
the secreted products, including lipids, terpenes, alkaloids and polysaccharides.
GC/MS phytochemical analysis of volatile compounds secreted by glandular trichomes, identified 42 compounds, representing >97% of the total volatiles in EO, obtained by hydrodistillation
in a Clevenger-type apparatus. The monoterpene fraction was dominant, with the prevalence of
oxygenated monoterpenes (38.5%), while the majority of sesquiterpene fraction referred to oxygenated sesquiterpenes (22.6%). Two dominant compounds, which determined the essential oil
chemotype, were trans-thujone (22.7%) and neryl-isovalerate (20.6%).
The essential oil protects the aerial parts of the plant against herbivores and pathogens and the
biological activity of the secreted products indicates their potential as pesticides or pharmaceuticals.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4323
ER  - 

@conference{
author = "Devrnja, Nina and Janošević, Dušica and Tešević, Vele and Savić, Jelena and Zdravković-Korać, Snežana and Milojević, Jelena and Ćalić, Dušica",
year = "2018",
abstract = "The morphology and structure of tansy glandular trichomes were characterized for the first
time using scanning electron microscopy and light microscopy, and their secretion was analyzed
by histochemical staining. Leaf indumentum of tansy was covered with non-glandular and glandular trichomes of biseriate type, distributed on both adaxial and abaxial surface. Both types of
trichomes were also distributed along the stem, but with lower density. Light microscopy showed
that biseriate glandular trichomes consisted of two broad basal cells embedded in the epidermis,
a short single-celled stalk, and a large round multicellular head. The histochemical analysis of the
glandular secretion, performed through several staining methods, revealed the complex nature of
the secreted products, including lipids, terpenes, alkaloids and polysaccharides.
GC/MS phytochemical analysis of volatile compounds secreted by glandular trichomes, identified 42 compounds, representing >97% of the total volatiles in EO, obtained by hydrodistillation
in a Clevenger-type apparatus. The monoterpene fraction was dominant, with the prevalence of
oxygenated monoterpenes (38.5%), while the majority of sesquiterpene fraction referred to oxygenated sesquiterpenes (22.6%). Two dominant compounds, which determined the essential oil
chemotype, were trans-thujone (22.7%) and neryl-isovalerate (20.6%).
The essential oil protects the aerial parts of the plant against herbivores and pathogens and the
biological activity of the secreted products indicates their potential as pesticides or pharmaceuticals.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4323"
}

Devrnja, N., Janošević, D., Tešević, V., Savić, J., Zdravković-Korać, S., Milojević, J.,& Ćalić, D.. (2018). Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 36.
https://hdl.handle.net/21.15107/rcub_ibiss_4323

Devrnja N, Janošević D, Tešević V, Savić J, Zdravković-Korać S, Milojević J, Ćalić D. Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:36.
https://hdl.handle.net/21.15107/rcub_ibiss_4323 .

Devrnja, Nina, Janošević, Dušica, Tešević, Vele, Savić, Jelena, Zdravković-Korać, Snežana, Milojević, Jelena, Ćalić, Dušica, "Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):36,
https://hdl.handle.net/21.15107/rcub_ibiss_4323 .

TY  - JOUR
AU  - Vidaković, Vera
AU  - Marković, Milena
AU  - Novaković, Miroslav
AU  - Jadranin, Milka
AU  - Popović, Zorica
AU  - Matić, Rada
AU  - Tešević, Vele
AU  - Bojović, Srđan
PY  - 2018
UR  - http://www.degruyter.com/view/j/hfsg.ahead-of-print/hf-2018-0019/hf-2018-0019.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3113
AB  - The reliability of diarylheptanoids as chemotaxonomic markers at inter- and intraspecific levels has been investigated. Six diarylheptanoids were quantified in bark ethanol extracts of four Alnus spp. populations by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The populations described here as locus classicus Alnus glutinosa (I) and locus classicus Alnus incana (III) are clearly differentiated. Compared to population I, individuals in population III have higher extract yields and hirsutanonol-5- O - β -D-glucopyranoside content and lower contents of hirsutanonol, rubranoside A and oregonin. Individuals from neighboring populations of A. glutinosa (II) and A. incana (IV) share similar contents of hirsutanonol. All the studied populations exhibit a high intrapopulation variability of the selected diarylheptanoids; they have a heterogeneous chemotype and they partially overlap. The geographical proximity of populations II and IV increases their chemical similarity. Principal component analysis (PCA) clearly shows that the biggest dispersion of individuals lies within population IV. The reason for its heterogeneity might be its physical proximity to population II, i.e. the appearance of hybrids. Also, geometric morphometrics of leaves was performed as a screening criterion for spontaneous hybrids.
T2  - Holzforschung
T1  - Inter- and intraspecific variability of selected diarylheptanoid compounds and leaf morphometric traits in Alnus glutinosa and Alnus incana
IS  - 12
VL  - 72
VL  - 12
DO  - 10.1515/hf-2018-0019
SP  - 1031
EP  - 1041
ER  - 

@article{
author = "Vidaković, Vera and Marković, Milena and Novaković, Miroslav and Jadranin, Milka and Popović, Zorica and Matić, Rada and Tešević, Vele and Bojović, Srđan",
year = "2018",
abstract = "The reliability of diarylheptanoids as chemotaxonomic markers at inter- and intraspecific levels has been investigated. Six diarylheptanoids were quantified in bark ethanol extracts of four Alnus spp. populations by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The populations described here as locus classicus Alnus glutinosa (I) and locus classicus Alnus incana (III) are clearly differentiated. Compared to population I, individuals in population III have higher extract yields and hirsutanonol-5- O - β -D-glucopyranoside content and lower contents of hirsutanonol, rubranoside A and oregonin. Individuals from neighboring populations of A. glutinosa (II) and A. incana (IV) share similar contents of hirsutanonol. All the studied populations exhibit a high intrapopulation variability of the selected diarylheptanoids; they have a heterogeneous chemotype and they partially overlap. The geographical proximity of populations II and IV increases their chemical similarity. Principal component analysis (PCA) clearly shows that the biggest dispersion of individuals lies within population IV. The reason for its heterogeneity might be its physical proximity to population II, i.e. the appearance of hybrids. Also, geometric morphometrics of leaves was performed as a screening criterion for spontaneous hybrids.",
journal = "Holzforschung",
title = "Inter- and intraspecific variability of selected diarylheptanoid compounds and leaf morphometric traits in Alnus glutinosa and Alnus incana",
number = "12",
volume = "72, 12",
doi = "10.1515/hf-2018-0019",
pages = "1031-1041"
}

Vidaković, V., Marković, M., Novaković, M., Jadranin, M., Popović, Z., Matić, R., Tešević, V.,& Bojović, S.. (2018). Inter- and intraspecific variability of selected diarylheptanoid compounds and leaf morphometric traits in Alnus glutinosa and Alnus incana. in Holzforschung, 72(12), 1031-1041.
https://doi.org/10.1515/hf-2018-0019

Vidaković V, Marković M, Novaković M, Jadranin M, Popović Z, Matić R, Tešević V, Bojović S. Inter- and intraspecific variability of selected diarylheptanoid compounds and leaf morphometric traits in Alnus glutinosa and Alnus incana. in Holzforschung. 2018;72(12):1031-1041.
doi:10.1515/hf-2018-0019 .

Vidaković, Vera, Marković, Milena, Novaković, Miroslav, Jadranin, Milka, Popović, Zorica, Matić, Rada, Tešević, Vele, Bojović, Srđan, "Inter- and intraspecific variability of selected diarylheptanoid compounds and leaf morphometric traits in Alnus glutinosa and Alnus incana" in Holzforschung, 72, no. 12 (2018):1031-1041,
https://doi.org/10.1515/hf-2018-0019 . .

TY  - JOUR
AU  - Popović, Zorica
AU  - Matić, Rada
AU  - Bajić-Ljubičić, Jasna
AU  - Tešević, Vele
AU  - Bojović, Srđan
PY  - 2018
UR  - http://link.springer.com/10.1007/s00468-017-1624-5
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2935
AB  - The aim of this study was to investigate the chemical variability of Cornus mas and Cornus sanguinea on the basis of the content of six selected phenolic compounds in fruit extracts. Fruits were sampled at the time of full ripening, mid-September, from two localities that differed in terms of orographic and environmental conditions. Fresh fruit extracts were analyzed by LC–MS/MS to determine the presence and contents of neochlorogenic acid, quercitrin, isoquercetin, hyperoside, rutoside and querciturone. While all of the tested compounds were detected in both species, their content was several times higher in C. sanguinea. Locality-dependent differences were tested by appropriate statistical tests, which confirmed that the contents of all compounds significantly differed in C. mas, whereas the content of only one compound differed in C. sanguinea. Differences between populations were also revealed by PCA analyses. Both species showed clear separation of populations based on the analyzed phenolic compounds. The results showed that a specific environment can significantly affect the contents of certain secondary metabolites, as in the case of C. mas, and/or that the synthesis of these compounds can be to a lesser extent influenced by external factors, as in the case of C. sanguinea.
T2  - Trees
T2  - Trees
T1  - Geographic variability of selected phenolic compounds in fresh berries of two Cornus species
IS  - 1
VL  - 32
DO  - 10.1007/s00468-017-1624-5
SP  - 203
EP  - 214
ER  - 

@article{
author = "Popović, Zorica and Matić, Rada and Bajić-Ljubičić, Jasna and Tešević, Vele and Bojović, Srđan",
year = "2018",
abstract = "The aim of this study was to investigate the chemical variability of Cornus mas and Cornus sanguinea on the basis of the content of six selected phenolic compounds in fruit extracts. Fruits were sampled at the time of full ripening, mid-September, from two localities that differed in terms of orographic and environmental conditions. Fresh fruit extracts were analyzed by LC–MS/MS to determine the presence and contents of neochlorogenic acid, quercitrin, isoquercetin, hyperoside, rutoside and querciturone. While all of the tested compounds were detected in both species, their content was several times higher in C. sanguinea. Locality-dependent differences were tested by appropriate statistical tests, which confirmed that the contents of all compounds significantly differed in C. mas, whereas the content of only one compound differed in C. sanguinea. Differences between populations were also revealed by PCA analyses. Both species showed clear separation of populations based on the analyzed phenolic compounds. The results showed that a specific environment can significantly affect the contents of certain secondary metabolites, as in the case of C. mas, and/or that the synthesis of these compounds can be to a lesser extent influenced by external factors, as in the case of C. sanguinea.",
journal = "Trees, Trees",
title = "Geographic variability of selected phenolic compounds in fresh berries of two Cornus species",
number = "1",
volume = "32",
doi = "10.1007/s00468-017-1624-5",
pages = "203-214"
}

Popović, Z., Matić, R., Bajić-Ljubičić, J., Tešević, V.,& Bojović, S.. (2018). Geographic variability of selected phenolic compounds in fresh berries of two Cornus species. in Trees, 32(1), 203-214.
https://doi.org/10.1007/s00468-017-1624-5

Popović Z, Matić R, Bajić-Ljubičić J, Tešević V, Bojović S. Geographic variability of selected phenolic compounds in fresh berries of two Cornus species. in Trees. 2018;32(1):203-214.
doi:10.1007/s00468-017-1624-5 .

Popović, Zorica, Matić, Rada, Bajić-Ljubičić, Jasna, Tešević, Vele, Bojović, Srđan, "Geographic variability of selected phenolic compounds in fresh berries of two Cornus species" in Trees, 32, no. 1 (2018):203-214,
https://doi.org/10.1007/s00468-017-1624-5 . .

TY  - JOUR
AU  - Vidaković, Vera
AU  - Novaković, Miroslav
AU  - Popović, Zorica
AU  - Janković, Milan
AU  - Matić, Rada
AU  - Tešević, Vele
AU  - Bojović, Srđan
PY  - 2018
UR  - http://www.degruyter.com/view/j/hfsg.2018.72.issue-1/hf-2017-0074/hf-2017-0074.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3112
AB  - Diarylheptanoids are a group of secondary metabolites widely distributed in the Betulaceae family and characteristic for Alnus species. In this study, the chemotaxonomic power of diarylheptanoids, such as hirsutanonol-5-O-β-d-glucopyranoside, rubranoside A, oregonin, platyphylloside, alnuside A and hirsutanonol, has been investigated in combination with principal component analysis (PCA) for differentiation of Alnus species. Concentrations of six diarylheptanoids in the bark extracts of two natural populations of Alnus glutinosa (black alder) and Alnus incana (gray alder) were determined by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). PCA clearly shows the separation of three groups. Populations I (A. glutinosa) and II (A. incana) both consisted of individuals of the corresponding species. Four individuals from both sampled populations formed a separate group (population III), which possibly represents a hybrid group. Accordingly, diarylheptanoids may serve in combination with PCA as chemotaxonomic markers at the species level, which may also reveal hybrid species.
T2  - Holzforschung
T1  - Significance of diarylheptanoids for chemotaxonomical distinguishing between Alnus glutinosa and Alnus incana
IS  - 1
VL  - 72
DO  - 10.1515/hf-2017-0074
SP  - 9
EP  - 16
ER  - 

@article{
author = "Vidaković, Vera and Novaković, Miroslav and Popović, Zorica and Janković, Milan and Matić, Rada and Tešević, Vele and Bojović, Srđan",
year = "2018",
abstract = "Diarylheptanoids are a group of secondary metabolites widely distributed in the Betulaceae family and characteristic for Alnus species. In this study, the chemotaxonomic power of diarylheptanoids, such as hirsutanonol-5-O-β-d-glucopyranoside, rubranoside A, oregonin, platyphylloside, alnuside A and hirsutanonol, has been investigated in combination with principal component analysis (PCA) for differentiation of Alnus species. Concentrations of six diarylheptanoids in the bark extracts of two natural populations of Alnus glutinosa (black alder) and Alnus incana (gray alder) were determined by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). PCA clearly shows the separation of three groups. Populations I (A. glutinosa) and II (A. incana) both consisted of individuals of the corresponding species. Four individuals from both sampled populations formed a separate group (population III), which possibly represents a hybrid group. Accordingly, diarylheptanoids may serve in combination with PCA as chemotaxonomic markers at the species level, which may also reveal hybrid species.",
journal = "Holzforschung",
title = "Significance of diarylheptanoids for chemotaxonomical distinguishing between Alnus glutinosa and Alnus incana",
number = "1",
volume = "72",
doi = "10.1515/hf-2017-0074",
pages = "9-16"
}

Vidaković, V., Novaković, M., Popović, Z., Janković, M., Matić, R., Tešević, V.,& Bojović, S.. (2018). Significance of diarylheptanoids for chemotaxonomical distinguishing between Alnus glutinosa and Alnus incana. in Holzforschung, 72(1), 9-16.
https://doi.org/10.1515/hf-2017-0074

Vidaković V, Novaković M, Popović Z, Janković M, Matić R, Tešević V, Bojović S. Significance of diarylheptanoids for chemotaxonomical distinguishing between Alnus glutinosa and Alnus incana. in Holzforschung. 2018;72(1):9-16.
doi:10.1515/hf-2017-0074 .

Vidaković, Vera, Novaković, Miroslav, Popović, Zorica, Janković, Milan, Matić, Rada, Tešević, Vele, Bojović, Srđan, "Significance of diarylheptanoids for chemotaxonomical distinguishing between Alnus glutinosa and Alnus incana" in Holzforschung, 72, no. 1 (2018):9-16,
https://doi.org/10.1515/hf-2017-0074 . .

TY  - JOUR
AU  - Thiesen, Letícia de Cassia Tavares
AU  - Sugauara, Elaine Yae Yamashita
AU  - Tešević, Vele
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Gonçalves, José Eduardo
AU  - Gazim, Zilda Cristiani
AU  - Linde, Giani Andrea
AU  - Colauto, Nelson Barros
PY  - 2017
UR  - http://www.funpecrp.com.br/gmr/year2017/vol16-2/pdf/gmr-16-02-gmr.16029548.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2740
AB  - Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of this oleoresin for seven bacteria and eight fungi were determined using 96-well microtiter plates. The oleoresin MBC for Bacillus cereus, Enterobacter cloacae, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus ranged from 0.01 to 0.08 mg/mL, whereas the controls streptomycin and ampicillin varied from 0.1 and 0.5 mg/ mL. The oleoresin MFC for Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and Trichoderma viride varied from 0.01 to 0.08 mg/mL, whereas the controls bifonazole and ketoconazole ranged from 0.2 to 3.5 mg/ mL. The oleoresin obtained by supercritical carbon dioxide presented bacteriostatic, bactericidal, fungistatic, and fungicidal activities that were higher than the positive controls streptomycin, ampicillin, bifonazole, and ketoconazole. The high antimicrobial activity was related to the high content of (E, E)-geranyllinalool that composes 21.0% of the oleoresin and a possible synergic action with fatty acid esters that made up 50.5% of the oleoresin. The oleoresin antimicrobial activity against common multiresistant bacteria in severe infectious processes as P. aeruginosa or against toxin-producing fungi such as P. ochrochloron or fungi that are difficult to control such as T. viride suggests the development of promising applications of this product in the food, farming, livestock, and pharmaceutical industry.
T2  - Genetics and Molecular Research
T1  - Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide
IS  - 2
VL  - 16
DO  - 10.4238/gmr16029548
SP  - gmr16029548
EP  - gmr16029548
ER  - 

@article{
author = "Thiesen, Letícia de Cassia Tavares and Sugauara, Elaine Yae Yamashita and Tešević, Vele and Glamočlija, Jasmina and Soković, Marina and Gonçalves, José Eduardo and Gazim, Zilda Cristiani and Linde, Giani Andrea and Colauto, Nelson Barros",
year = "2017",
abstract = "Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of this oleoresin for seven bacteria and eight fungi were determined using 96-well microtiter plates. The oleoresin MBC for Bacillus cereus, Enterobacter cloacae, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus ranged from 0.01 to 0.08 mg/mL, whereas the controls streptomycin and ampicillin varied from 0.1 and 0.5 mg/ mL. The oleoresin MFC for Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and Trichoderma viride varied from 0.01 to 0.08 mg/mL, whereas the controls bifonazole and ketoconazole ranged from 0.2 to 3.5 mg/ mL. The oleoresin obtained by supercritical carbon dioxide presented bacteriostatic, bactericidal, fungistatic, and fungicidal activities that were higher than the positive controls streptomycin, ampicillin, bifonazole, and ketoconazole. The high antimicrobial activity was related to the high content of (E, E)-geranyllinalool that composes 21.0% of the oleoresin and a possible synergic action with fatty acid esters that made up 50.5% of the oleoresin. The oleoresin antimicrobial activity against common multiresistant bacteria in severe infectious processes as P. aeruginosa or against toxin-producing fungi such as P. ochrochloron or fungi that are difficult to control such as T. viride suggests the development of promising applications of this product in the food, farming, livestock, and pharmaceutical industry.",
journal = "Genetics and Molecular Research",
title = "Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide",
number = "2",
volume = "16",
doi = "10.4238/gmr16029548",
pages = "gmr16029548-gmr16029548"
}

Thiesen, L. d. C. T., Sugauara, E. Y. Y., Tešević, V., Glamočlija, J., Soković, M., Gonçalves, J. E., Gazim, Z. C., Linde, G. A.,& Colauto, N. B.. (2017). Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide. in Genetics and Molecular Research, 16(2), gmr16029548-gmr16029548.
https://doi.org/10.4238/gmr16029548

Thiesen LDCT, Sugauara EYY, Tešević V, Glamočlija J, Soković M, Gonçalves JE, Gazim ZC, Linde GA, Colauto NB. Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide. in Genetics and Molecular Research. 2017;16(2):gmr16029548-gmr16029548.
doi:10.4238/gmr16029548 .

Thiesen, Letícia de Cassia Tavares, Sugauara, Elaine Yae Yamashita, Tešević, Vele, Glamočlija, Jasmina, Soković, Marina, Gonçalves, José Eduardo, Gazim, Zilda Cristiani, Linde, Giani Andrea, Colauto, Nelson Barros, "Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide" in Genetics and Molecular Research, 16, no. 2 (2017):gmr16029548-gmr16029548,
https://doi.org/10.4238/gmr16029548 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Perić Mataruga, Vesna
AU  - Ilijin, Larisa
AU  - Tešević, Vele
AU  - Todosijević, Marina
AU  - Vujisić, Ljubodrag
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2017
UR  - http://link.springer.com/10.1007/s00253-016-8082-7
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-85008708376&origin=SingleRecordEmailAlert&dgcid=scalert_sc_search_email&txGid=BCBFF82A73D51FA0ED62BC41FE5E5987.wsnAw8kcdt7IPYLO0V48gA%3A37
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2511
AB  - Recently, various insect secretions have been tested as possible antimicrobial agents. In beetles, these secretions are essentially products of various exocrine glands, of which particular emphasis is on pygidial glands that are common for the suborder Adephaga. The antimicrobial potential of the pygidial gland secretion isolated from the adults of Calosoma sycophanta against human pathogens has been tested and compared with the potential of other carabid species, particularly within the tribe Carabini. The antimicrobial assay includes a microdilution method which was applied in order to determine the minimal inhibitory, minimal fungicidal and minimal bactericidal concentrations. It has been tested the effect of the secretion against eight strains of fungal and eight strains of bacterial species. The secretion sample of the tested carabid species has shown statistically significant antifungal effect against all strains of treated micromycetes, the highest in comparison with previously tested carabids (Carabus spp., Laemostenus punctatus and Abax parallelepipedus). Aspergillus versicolor proved to be the most sensitive micromycete, while the remaining seven fungal strains have shown the same level of sensibility. In comparison with commercial mycotics ketoconazole and bifonazole, applied as positive controls, the tested secretion showed much higher antifungal activity for all fungal strains. Antibacterial effect has been manifested only against one bacterial strain (Escherichia coli), contrary to other previously studied carabid species. These observations might have a significant impact on the ecological domain and possible purpose in biomedical studies and applications in the future. Additionally, morpho-histology of the pygidial glands of C. sycophanta is investigated and discussed.
T2  - Applied Microbiology and Biotechnology
T1  - The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens
IS  - 3
VL  - 101
DO  - 10.1007/s00253-016-8082-7
SP  - 977
EP  - 985
ER  - 

@article{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Perić Mataruga, Vesna and Ilijin, Larisa and Tešević, Vele and Todosijević, Marina and Vujisić, Ljubodrag and Vesović, Nikola and Ćurčić, Srećko",
year = "2017",
abstract = "Recently, various insect secretions have been tested as possible antimicrobial agents. In beetles, these secretions are essentially products of various exocrine glands, of which particular emphasis is on pygidial glands that are common for the suborder Adephaga. The antimicrobial potential of the pygidial gland secretion isolated from the adults of Calosoma sycophanta against human pathogens has been tested and compared with the potential of other carabid species, particularly within the tribe Carabini. The antimicrobial assay includes a microdilution method which was applied in order to determine the minimal inhibitory, minimal fungicidal and minimal bactericidal concentrations. It has been tested the effect of the secretion against eight strains of fungal and eight strains of bacterial species. The secretion sample of the tested carabid species has shown statistically significant antifungal effect against all strains of treated micromycetes, the highest in comparison with previously tested carabids (Carabus spp., Laemostenus punctatus and Abax parallelepipedus). Aspergillus versicolor proved to be the most sensitive micromycete, while the remaining seven fungal strains have shown the same level of sensibility. In comparison with commercial mycotics ketoconazole and bifonazole, applied as positive controls, the tested secretion showed much higher antifungal activity for all fungal strains. Antibacterial effect has been manifested only against one bacterial strain (Escherichia coli), contrary to other previously studied carabid species. These observations might have a significant impact on the ecological domain and possible purpose in biomedical studies and applications in the future. Additionally, morpho-histology of the pygidial glands of C. sycophanta is investigated and discussed.",
journal = "Applied Microbiology and Biotechnology",
title = "The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens",
number = "3",
volume = "101",
doi = "10.1007/s00253-016-8082-7",
pages = "977-985"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Perić Mataruga, V., Ilijin, L., Tešević, V., Todosijević, M., Vujisić, L., Vesović, N.,& Ćurčić, S.. (2017). The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens. in Applied Microbiology and Biotechnology, 101(3), 977-985.
https://doi.org/10.1007/s00253-016-8082-7

Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić Mataruga V, Ilijin L, Tešević V, Todosijević M, Vujisić L, Vesović N, Ćurčić S. The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens. in Applied Microbiology and Biotechnology. 2017;101(3):977-985.
doi:10.1007/s00253-016-8082-7 .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Perić Mataruga, Vesna, Ilijin, Larisa, Tešević, Vele, Todosijević, Marina, Vujisić, Ljubodrag, Vesović, Nikola, Ćurčić, Srećko, "The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens" in Applied Microbiology and Biotechnology, 101, no. 3 (2017):977-985,
https://doi.org/10.1007/s00253-016-8082-7 . .