TY  - JOUR
AU  - Alimpić-Aradski, Ana
AU  - Olađe Pavlović, Mariana
AU  - Janošević, Dušica
AU  - Todorović, Slađana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Pljevljakušić, Dejan
AU  - Šavikin, Katarina
AU  - Marin, Petar D.
AU  - Giweli, Abdulhmid
AU  - Duletić-Laušević, Sonja
PY  - 2023
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/5152
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6445
AB  - Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.
PB  - Hoboken: John Wiley and Sons
T2  - Phytochemical Analysis
T1  - Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)
IS  - 6
VL  - 34
DO  - 10.1002/pca.3257
SP  - 661
EP  - 679
ER  - 

@article{
author = "Alimpić-Aradski, Ana and Olađe Pavlović, Mariana and Janošević, Dušica and Todorović, Slađana and Gašić, Uroš and Mišić, Danijela and Pljevljakušić, Dejan and Šavikin, Katarina and Marin, Petar D. and Giweli, Abdulhmid and Duletić-Laušević, Sonja",
year = "2023",
abstract = "Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Phytochemical Analysis",
title = "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)",
number = "6",
volume = "34",
doi = "10.1002/pca.3257",
pages = "661-679"
}

Alimpić-Aradski, A., Olađe Pavlović, M., Janošević, D., Todorović, S., Gašić, U., Mišić, D., Pljevljakušić, D., Šavikin, K., Marin, P. D., Giweli, A.,& Duletić-Laušević, S.. (2023). Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis
Hoboken: John Wiley and Sons., 34(6), 661-679.
https://doi.org/10.1002/pca.3257

Alimpić-Aradski A, Olađe Pavlović M, Janošević D, Todorović S, Gašić U, Mišić D, Pljevljakušić D, Šavikin K, Marin PD, Giweli A, Duletić-Laušević S. Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis. 2023;34(6):661-679.
doi:10.1002/pca.3257 .

Alimpić-Aradski, Ana, Olađe Pavlović, Mariana, Janošević, Dušica, Todorović, Slađana, Gašić, Uroš, Mišić, Danijela, Pljevljakušić, Dejan, Šavikin, Katarina, Marin, Petar D., Giweli, Abdulhmid, Duletić-Laušević, Sonja, "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)" in Phytochemical Analysis, 34, no. 6 (2023):661-679,
https://doi.org/10.1002/pca.3257 . .

TY  - CONF
AU  - Glamočlija, Jasmina
AU  - Stanojković, Jelena
AU  - Janošević, Dušica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6370
AB  - The oldest data on hypogeous fungi from the territory of Serbia date
back to the year of 1777, but first study on truffles and truffle-like fungi in Serbia
started in 1992. Collected material, fruit bodies of Tuber species had been
founded and identified and extensively studied during the last decade of the
twentieth century.
Despite the current interest in truffles in Serbia, there is not much
information about artificially established truffle plantations. The register
orchards of truffles in Serbia have not been created yet.
Thus, the objective of this study was the morphological and anatomical
characterization of an unknown ectomycorrhizas of host species Populus alba
from cultivated seedlings from part of a ten-year-old truffles plantation.
In autumn 2016, we were invited to visit and determine what kind of
mycorrhizae is situated in the plantation near the town of Požarevac (Eastern
Serbia). The truffle-ground (about 3,000 m2) is placed in Kličevac where a
plantation was established during the autumn 2007. According to the owner, he
bought commercially produced seedlings of Populus alba inoculated with the
black truffle.
PB  - Belgrade: Institute for Multidisciplinary Research, University of Belgrade
C3  - Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia
T1  - Characterization of Tuber macrosporum / Populus alba mycorrhizas from cultivated seedlings: case report
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6370
ER  - 

@conference{
author = "Glamočlija, Jasmina and Stanojković, Jelena and Janošević, Dušica",
year = "2023",
abstract = "The oldest data on hypogeous fungi from the territory of Serbia date
back to the year of 1777, but first study on truffles and truffle-like fungi in Serbia
started in 1992. Collected material, fruit bodies of Tuber species had been
founded and identified and extensively studied during the last decade of the
twentieth century.
Despite the current interest in truffles in Serbia, there is not much
information about artificially established truffle plantations. The register
orchards of truffles in Serbia have not been created yet.
Thus, the objective of this study was the morphological and anatomical
characterization of an unknown ectomycorrhizas of host species Populus alba
from cultivated seedlings from part of a ten-year-old truffles plantation.
In autumn 2016, we were invited to visit and determine what kind of
mycorrhizae is situated in the plantation near the town of Požarevac (Eastern
Serbia). The truffle-ground (about 3,000 m2) is placed in Kličevac where a
plantation was established during the autumn 2007. According to the owner, he
bought commercially produced seedlings of Populus alba inoculated with the
black truffle.",
publisher = "Belgrade: Institute for Multidisciplinary Research, University of Belgrade",
journal = "Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia",
title = "Characterization of Tuber macrosporum / Populus alba mycorrhizas from cultivated seedlings: case report",
pages = "37",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6370"
}

Glamočlija, J., Stanojković, J.,& Janošević, D.. (2023). Characterization of Tuber macrosporum / Populus alba mycorrhizas from cultivated seedlings: case report. in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia
Belgrade: Institute for Multidisciplinary Research, University of Belgrade., 37.
https://hdl.handle.net/21.15107/rcub_ibiss_6370

Glamočlija J, Stanojković J, Janošević D. Characterization of Tuber macrosporum / Populus alba mycorrhizas from cultivated seedlings: case report. in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia. 2023;:37.
https://hdl.handle.net/21.15107/rcub_ibiss_6370 .

Glamočlija, Jasmina, Stanojković, Jelena, Janošević, Dušica, "Characterization of Tuber macrosporum / Populus alba mycorrhizas from cultivated seedlings: case report" in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia (2023):37,
https://hdl.handle.net/21.15107/rcub_ibiss_6370 .

TY  - GEN
AU  - Marjanović, Žaklina
AU  - Glišić, Aleksa
AU  - Stefanović, Slobodan
AU  - Glamočlija, Jasmina
AU  - Janošević, Dušica
AU  - Saljnikov, Elmira
AU  - Leonardi, Marco
AU  - Paccioni, Giovanni
AU  - Iotti, Mirco
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6203
AB  - Truffle diversity of Balkan Peninsula does not stop to surprise the
scientific community with three new species from the melanosporum clade
described in last year's (Mereny et al 2017, Milenković at al 2015, Slavova et al
2023). Out of large set of ITS sequences, Mereny at al. (2017) described T.
cryptobrumale as connected to Pannonian region, but two other genetically quite
distinctive populations were not distinguished as species. In this contribution, we
have analyzed larger set of sequences and their habitats and detected strong
connection between specimens with specific ITS/LSU region variants to
ecologically specific habitats of Balkan peninsula. Soils and vegetation where
these specimens have been detected rather resemble descriptions of T.
melanosporum sites in Italy, while habitats of T. cryptobrumale resemble those of
T. magnatum. Additionally, we have detected numerous populations of T.
melanosporum in the coastal regions of Croatia, while this species has never been
officially detected eastern from Dinaric alps in Balkan peninsula. We hypothesize
that T. melanosporum could never overcome low temperatures of high and long
Dinaric massive and is therefore localized in the western Mediterranean regions.
This could be the reason why the melanosporum clade has been highly
diversifying in Balkan regions. Moreover, the genetic variant widely distributed
all over Europe and described as “T. brumale” has never been detected in the
Balkans. Therefore, we propose that ecologically and genetically specific taxon
that is widespread only in Balkan peninsula (and to some extent northwards)
should be recognized as a different species - Tuber balkanicum.
PB  - Belgrade: Institute for Multidisciplinary Research, University of Belgrade
T2  - Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia
T1  - Tuber balcanicum – new taxon defined by molecular, ecological and biogeographical methods
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6203
ER  - 

@misc{
author = "Marjanović, Žaklina and Glišić, Aleksa and Stefanović, Slobodan and Glamočlija, Jasmina and Janošević, Dušica and Saljnikov, Elmira and Leonardi, Marco and Paccioni, Giovanni and Iotti, Mirco",
year = "2023",
abstract = "Truffle diversity of Balkan Peninsula does not stop to surprise the
scientific community with three new species from the melanosporum clade
described in last year's (Mereny et al 2017, Milenković at al 2015, Slavova et al
2023). Out of large set of ITS sequences, Mereny at al. (2017) described T.
cryptobrumale as connected to Pannonian region, but two other genetically quite
distinctive populations were not distinguished as species. In this contribution, we
have analyzed larger set of sequences and their habitats and detected strong
connection between specimens with specific ITS/LSU region variants to
ecologically specific habitats of Balkan peninsula. Soils and vegetation where
these specimens have been detected rather resemble descriptions of T.
melanosporum sites in Italy, while habitats of T. cryptobrumale resemble those of
T. magnatum. Additionally, we have detected numerous populations of T.
melanosporum in the coastal regions of Croatia, while this species has never been
officially detected eastern from Dinaric alps in Balkan peninsula. We hypothesize
that T. melanosporum could never overcome low temperatures of high and long
Dinaric massive and is therefore localized in the western Mediterranean regions.
This could be the reason why the melanosporum clade has been highly
diversifying in Balkan regions. Moreover, the genetic variant widely distributed
all over Europe and described as “T. brumale” has never been detected in the
Balkans. Therefore, we propose that ecologically and genetically specific taxon
that is widespread only in Balkan peninsula (and to some extent northwards)
should be recognized as a different species - Tuber balkanicum.",
publisher = "Belgrade: Institute for Multidisciplinary Research, University of Belgrade",
journal = "Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia",
title = "Tuber balcanicum – new taxon defined by molecular, ecological and biogeographical methods",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6203"
}

Marjanović, Ž., Glišić, A., Stefanović, S., Glamočlija, J., Janošević, D., Saljnikov, E., Leonardi, M., Paccioni, G.,& Iotti, M.. (2023). Tuber balcanicum – new taxon defined by molecular, ecological and biogeographical methods. in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia
Belgrade: Institute for Multidisciplinary Research, University of Belgrade., 19.
https://hdl.handle.net/21.15107/rcub_ibiss_6203

Marjanović Ž, Glišić A, Stefanović S, Glamočlija J, Janošević D, Saljnikov E, Leonardi M, Paccioni G, Iotti M. Tuber balcanicum – new taxon defined by molecular, ecological and biogeographical methods. in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia. 2023;:19.
https://hdl.handle.net/21.15107/rcub_ibiss_6203 .

Marjanović, Žaklina, Glišić, Aleksa, Stefanović, Slobodan, Glamočlija, Jasmina, Janošević, Dušica, Saljnikov, Elmira, Leonardi, Marco, Paccioni, Giovanni, Iotti, Mirco, "Tuber balcanicum – new taxon defined by molecular, ecological and biogeographical methods" in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia (2023):19,
https://hdl.handle.net/21.15107/rcub_ibiss_6203 .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Ćirić, Ana
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Anđelković, Boban
AU  - Todosijević, Marina
AU  - Todorović, Slađana
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Tešević, Vele
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Kanellis, Angelos K.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669022004757
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4983
AB  - Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites , such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus . Histochemical staining of leaves' trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus , grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) and two Gram-positive bacteria ( Bacillus cereus and Micrococcus flavus ). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent -3 β -acetoxy-13- epi -manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent -3 β -acetoxy-13- epi -manoyl oxide and its precursor ent -3 β -hydroxy-13- epi -manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”
VL  - 184
DO  - 10.1016/j.indcrop.2022.114992
SP  - 114992
ER  - 

@article{
author = "Skorić, Marijana and Ćirić, Ana and Budimir, Snežana and Janošević, Dušica and Anđelković, Boban and Todosijević, Marina and Todorović, Slađana and Soković, Marina and Glamočlija, Jasmina and Tešević, Vele and Gašić, Uroš and Mišić, Danijela and Kanellis, Angelos K.",
year = "2022",
abstract = "Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites , such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus . Histochemical staining of leaves' trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus , grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) and two Gram-positive bacteria ( Bacillus cereus and Micrococcus flavus ). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent -3 β -acetoxy-13- epi -manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent -3 β -acetoxy-13- epi -manoyl oxide and its precursor ent -3 β -hydroxy-13- epi -manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”",
volume = "184",
doi = "10.1016/j.indcrop.2022.114992",
pages = "114992"
}

Skorić, M., Ćirić, A., Budimir, S., Janošević, D., Anđelković, B., Todosijević, M., Todorović, S., Soković, M., Glamočlija, J., Tešević, V., Gašić, U., Mišić, D.,& Kanellis, A. K.. (2022). Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products
Elsevier B.V.., 184, 114992.
https://doi.org/10.1016/j.indcrop.2022.114992

Skorić M, Ćirić A, Budimir S, Janošević D, Anđelković B, Todosijević M, Todorović S, Soković M, Glamočlija J, Tešević V, Gašić U, Mišić D, Kanellis AK. Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products. 2022;184:114992.
doi:10.1016/j.indcrop.2022.114992 .

Skorić, Marijana, Ćirić, Ana, Budimir, Snežana, Janošević, Dušica, Anđelković, Boban, Todosijević, Marina, Todorović, Slađana, Soković, Marina, Glamočlija, Jasmina, Tešević, Vele, Gašić, Uroš, Mišić, Danijela, Kanellis, Angelos K., "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”" in Industrial Crops and Products, 184 (2022):114992,
https://doi.org/10.1016/j.indcrop.2022.114992 . .

TY  - CONF
AU  - Cvetić Antić, Tijana
AU  - Janošević, Dušica
AU  - Glamočlija, Jasmina
AU  - Mitrović, Aleksandra
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6503
AB  - Fungi and plants interact in different ways, creating a scale of associations. Hemibiotrophic
fungi represent the most interesting group, as they use sequential biotrophic and necrotrophic infection strategies. The co-evolution of plant and fungal life-styles has not been well characterized.
We present changes of enzymatic component of antioxidative system in succulent plant Tacitus
bellus that specifically correspond to subsequent phases (spore germination, biotrophic phase,
and necrotrophic phase) of hemibiotroph Fusarium verticillioides infection. T. bellus response to F.
verticillioides spore germination was characterized by transient increase in catalase (CAT), but decrease in superoxide dismutase (SOD) and peroxidase (POD) activity. During biotrophic phase of F.
verticillioides infection, when hyphae spread intercellularly in epidermal and mesophyll tissue, host
antioxidative system was suppressed. The transition from biotrophic to necrotrophic phase (inter
and intracellular colonization and sporulation) triggered the host plant cells to create a highly defensive environment: CAT, SOD and POD activities were significantly stimulated, slowing, or even
currently arresting, colonization of T. bellus mesophyll cells. CAT, showing the most pronounced
activity increase, could be suggested as the main enzyme responsible for slowing the progression
of necrotrophic phase of F. verticillioides growth. However, contrary to host CAT and SOD which
isoenzyme profile didn’t change, new highly acidic POD isoforms replaced the two mildly acidic
isoforms, suggesting their specific role in slowing the progression of infection. Presented results
add to knowledge of events and mechanisms related to hemibiotrophic fungi pathogenicity in
succulent plants grown under high relative humidity, similar to conditions in greenhouse.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6503
ER  - 

@conference{
author = "Cvetić Antić, Tijana and Janošević, Dušica and Glamočlija, Jasmina and Mitrović, Aleksandra",
year = "2022",
abstract = "Fungi and plants interact in different ways, creating a scale of associations. Hemibiotrophic
fungi represent the most interesting group, as they use sequential biotrophic and necrotrophic infection strategies. The co-evolution of plant and fungal life-styles has not been well characterized.
We present changes of enzymatic component of antioxidative system in succulent plant Tacitus
bellus that specifically correspond to subsequent phases (spore germination, biotrophic phase,
and necrotrophic phase) of hemibiotroph Fusarium verticillioides infection. T. bellus response to F.
verticillioides spore germination was characterized by transient increase in catalase (CAT), but decrease in superoxide dismutase (SOD) and peroxidase (POD) activity. During biotrophic phase of F.
verticillioides infection, when hyphae spread intercellularly in epidermal and mesophyll tissue, host
antioxidative system was suppressed. The transition from biotrophic to necrotrophic phase (inter
and intracellular colonization and sporulation) triggered the host plant cells to create a highly defensive environment: CAT, SOD and POD activities were significantly stimulated, slowing, or even
currently arresting, colonization of T. bellus mesophyll cells. CAT, showing the most pronounced
activity increase, could be suggested as the main enzyme responsible for slowing the progression
of necrotrophic phase of F. verticillioides growth. However, contrary to host CAT and SOD which
isoenzyme profile didn’t change, new highly acidic POD isoforms replaced the two mildly acidic
isoforms, suggesting their specific role in slowing the progression of infection. Presented results
add to knowledge of events and mechanisms related to hemibiotrophic fungi pathogenicity in
succulent plants grown under high relative humidity, similar to conditions in greenhouse.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6503"
}

Cvetić Antić, T., Janošević, D., Glamočlija, J.,& Mitrović, A.. (2022). Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 50.
https://hdl.handle.net/21.15107/rcub_ibiss_6503

Cvetić Antić T, Janošević D, Glamočlija J, Mitrović A. Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:50.
https://hdl.handle.net/21.15107/rcub_ibiss_6503 .

Cvetić Antić, Tijana, Janošević, Dušica, Glamočlija, Jasmina, Mitrović, Aleksandra, "Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):50,
https://hdl.handle.net/21.15107/rcub_ibiss_6503 .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Krstić Milošević, Dijana
AU  - Janošević, Dušica
AU  - Tešević, Vele
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
AU  - Ćalić, Dušica
PY  - 2021
UR  - http://link.springer.com/10.1007/s00709-020-01588-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4038
AB  - In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.
PB  - Springer
T2  - Protoplasma
T1  - In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents
IS  - 3
VL  - 258
DO  - 10.1007/s00709-020-01588-9
SP  - 587
EP  - 599
ER  - 

@article{
author = "Devrnja, Nina and Krstić Milošević, Dijana and Janošević, Dušica and Tešević, Vele and Vinterhalter, Branka and Savić, Jelena and Ćalić, Dušica",
year = "2021",
abstract = "In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.",
publisher = "Springer",
journal = "Protoplasma",
title = "In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents",
number = "3",
volume = "258",
doi = "10.1007/s00709-020-01588-9",
pages = "587-599"
}

Devrnja, N., Krstić Milošević, D., Janošević, D., Tešević, V., Vinterhalter, B., Savić, J.,& Ćalić, D.. (2021). In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents. in Protoplasma
Springer., 258(3), 587-599.
https://doi.org/10.1007/s00709-020-01588-9

Devrnja N, Krstić Milošević D, Janošević D, Tešević V, Vinterhalter B, Savić J, Ćalić D. In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents. in Protoplasma. 2021;258(3):587-599.
doi:10.1007/s00709-020-01588-9 .

Devrnja, Nina, Krstić Milošević, Dijana, Janošević, Dušica, Tešević, Vele, Vinterhalter, Branka, Savić, Jelena, Ćalić, Dušica, "In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents" in Protoplasma, 258, no. 3 (2021):587-599,
https://doi.org/10.1007/s00709-020-01588-9 . .

TY  - JOUR
AU  - Alimpić Aradski, Ana
AU  - Janošević, Dušica
AU  - Pećinar, Ilinka
AU  - Budimir, Snežana
AU  - Dajić Stevanović, Zora
AU  - Matevski, Vlado
AU  - Marin, Petar
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3631
AB  - In this study, we examined Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. collected in the Republic of North Macedonia, which are for the first time subjected to detailed micromorphological and structural analysis using light and scanning electron microscopy. The nutlets and mucilage were additionally subjected to spectroscopic analysis using Raman and Attenuated Total Reflectance Fourier Transform Infrared spectroscopy. The anatomical structure of stems and leaves is described and compared. The stems, leaves and calyces bear numerous one- and multi-cellular nonglandular trichomes, and various peltate, capitate and digitiform glandular trichomes. The nutlets differ in size and shape, as well as in myxocarpy. The nutlets predominantly contained α-linolenic and linoleic acid, whereas the mucilages are primarily formed of polysaccharides. The results obtained in this study confirmed the importance of micromorphological and anatomical analysis of Salvia L. spp. plant parts, particularly trichomes and nutlets, and hence contributing to the knowledge about the variety of micromorphological characteristics within the genus Salvia L.
PB  - Taylor and Francis Ltd.
T2  - Plant Biosystems
T1  - Micromorphological and anatomical characteristics of Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. (Lamiaceae)
IS  - 1
VL  - 155
DO  - 10.1080/11263504.2020.1727976
SP  - 92
EP  - 108
ER  - 

@article{
author = "Alimpić Aradski, Ana and Janošević, Dušica and Pećinar, Ilinka and Budimir, Snežana and Dajić Stevanović, Zora and Matevski, Vlado and Marin, Petar and Duletić-Laušević, Sonja",
year = "2021",
abstract = "In this study, we examined Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. collected in the Republic of North Macedonia, which are for the first time subjected to detailed micromorphological and structural analysis using light and scanning electron microscopy. The nutlets and mucilage were additionally subjected to spectroscopic analysis using Raman and Attenuated Total Reflectance Fourier Transform Infrared spectroscopy. The anatomical structure of stems and leaves is described and compared. The stems, leaves and calyces bear numerous one- and multi-cellular nonglandular trichomes, and various peltate, capitate and digitiform glandular trichomes. The nutlets differ in size and shape, as well as in myxocarpy. The nutlets predominantly contained α-linolenic and linoleic acid, whereas the mucilages are primarily formed of polysaccharides. The results obtained in this study confirmed the importance of micromorphological and anatomical analysis of Salvia L. spp. plant parts, particularly trichomes and nutlets, and hence contributing to the knowledge about the variety of micromorphological characteristics within the genus Salvia L.",
publisher = "Taylor and Francis Ltd.",
journal = "Plant Biosystems",
title = "Micromorphological and anatomical characteristics of Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. (Lamiaceae)",
number = "1",
volume = "155",
doi = "10.1080/11263504.2020.1727976",
pages = "92-108"
}

Alimpić Aradski, A., Janošević, D., Pećinar, I., Budimir, S., Dajić Stevanović, Z., Matevski, V., Marin, P.,& Duletić-Laušević, S.. (2021). Micromorphological and anatomical characteristics of Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. (Lamiaceae). in Plant Biosystems
Taylor and Francis Ltd.., 155(1), 92-108.
https://doi.org/10.1080/11263504.2020.1727976

Alimpić Aradski A, Janošević D, Pećinar I, Budimir S, Dajić Stevanović Z, Matevski V, Marin P, Duletić-Laušević S. Micromorphological and anatomical characteristics of Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. (Lamiaceae). in Plant Biosystems. 2021;155(1):92-108.
doi:10.1080/11263504.2020.1727976 .

Alimpić Aradski, Ana, Janošević, Dušica, Pećinar, Ilinka, Budimir, Snežana, Dajić Stevanović, Zora, Matevski, Vlado, Marin, Petar, Duletić-Laušević, Sonja, "Micromorphological and anatomical characteristics of Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. (Lamiaceae)" in Plant Biosystems, 155, no. 1 (2021):92-108,
https://doi.org/10.1080/11263504.2020.1727976 . .

TY  - JOUR
AU  - Stanojković, Jelena
AU  - Todorović, Slađana
AU  - Pećinar, Ilinka
AU  - Lević, Steva
AU  - Ćalić, Snežana
AU  - Janošević, Dušica
PY  - 2020
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669020310189
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4071
AB  - Glandular trichomes are secretory organs that vary greatly in size, shape, location, type, and composition of secondary metabolites that are synthesized in them. They represent a protective chemical barrier and metabolic factories for the synthesis of bioactive compounds. We evaluated the effect of sucrose on the growth and biomass production, as well as on the distribution, density, and chemical composition of leaf glandular trichomes developed in vitro cultured Inula britannica, an important medical and aromatic species. Nodal segments (5 mm) were cultivated on the basal Murashige and Skoog medium with different concentrations (0 M, 0.01 M, 0.06 M, 0.1 M and 0.3 M) of sucrose. The lack of sugar does not retard growth, while a high sugar concentration inhibits biomass accumulation in vitro. The density and number of leaf glandular trichomes are changed with the medium composition variation. Morphoanatomical and histochemical analyses of the trichomes of the cultured I. britannica applying SEM, conventional light, fluorescent and Raman microscopy revealed the presence of non- glandular and biseriate glandular trichomes on the leaf surface. The histochemical analysis proved that glandular trichomes synthesized a complex mixture of biomolecules. The Raman microscopy analysis of glandular trichomes confirmed the presence of terpenes, most probably the bicyclic monoterpenes and sesquiterpenes. The multivariate analysis, based on PCA, was applied to distinguish among the glandular trichomes of three sugar concentrations: sugar-free medium (0 M), optimal (0.1 M) and the highest sucrose concentration (0.3 M). The main differences between the chemical profile of the leaf glandular trichomes of the plants grown on the 0 M and the chemical profile of the trichomes developed on the 0.1 and 0.3 M medium come mainly from phenolic compounds and to a smaller degree from cyclic terpenes, while the chemical profile of the glandular trichomes grown on the 0.1 M and 0.3 M differs considerably from the trichomes grown on the sugar-free medium in the polysaccharide cell wall ingredients. Most differences between the chemical profile of the glandular trichomes developed on 0 M and 0.3 M and the chemical profile of the trichomes grown on the 0.1 M of sucrose, come from phenolic compounds.
PB  - Elsevier BV
T2  - Industrial Crops and Products
T1  - Leaf glandular trichomes of micropropagated Inula britannica – Effect of sucrose on trichome density, distribution and chemical profile
DO  - 10.1016/j.indcrop.2020.113101
SP  - 113101
ER  - 

@article{
author = "Stanojković, Jelena and Todorović, Slađana and Pećinar, Ilinka and Lević, Steva and Ćalić, Snežana and Janošević, Dušica",
year = "2020",
abstract = "Glandular trichomes are secretory organs that vary greatly in size, shape, location, type, and composition of secondary metabolites that are synthesized in them. They represent a protective chemical barrier and metabolic factories for the synthesis of bioactive compounds. We evaluated the effect of sucrose on the growth and biomass production, as well as on the distribution, density, and chemical composition of leaf glandular trichomes developed in vitro cultured Inula britannica, an important medical and aromatic species. Nodal segments (5 mm) were cultivated on the basal Murashige and Skoog medium with different concentrations (0 M, 0.01 M, 0.06 M, 0.1 M and 0.3 M) of sucrose. The lack of sugar does not retard growth, while a high sugar concentration inhibits biomass accumulation in vitro. The density and number of leaf glandular trichomes are changed with the medium composition variation. Morphoanatomical and histochemical analyses of the trichomes of the cultured I. britannica applying SEM, conventional light, fluorescent and Raman microscopy revealed the presence of non- glandular and biseriate glandular trichomes on the leaf surface. The histochemical analysis proved that glandular trichomes synthesized a complex mixture of biomolecules. The Raman microscopy analysis of glandular trichomes confirmed the presence of terpenes, most probably the bicyclic monoterpenes and sesquiterpenes. The multivariate analysis, based on PCA, was applied to distinguish among the glandular trichomes of three sugar concentrations: sugar-free medium (0 M), optimal (0.1 M) and the highest sucrose concentration (0.3 M). The main differences between the chemical profile of the leaf glandular trichomes of the plants grown on the 0 M and the chemical profile of the trichomes developed on the 0.1 and 0.3 M medium come mainly from phenolic compounds and to a smaller degree from cyclic terpenes, while the chemical profile of the glandular trichomes grown on the 0.1 M and 0.3 M differs considerably from the trichomes grown on the sugar-free medium in the polysaccharide cell wall ingredients. Most differences between the chemical profile of the glandular trichomes developed on 0 M and 0.3 M and the chemical profile of the trichomes grown on the 0.1 M of sucrose, come from phenolic compounds.",
publisher = "Elsevier BV",
journal = "Industrial Crops and Products",
title = "Leaf glandular trichomes of micropropagated Inula britannica – Effect of sucrose on trichome density, distribution and chemical profile",
doi = "10.1016/j.indcrop.2020.113101",
pages = "113101"
}

Stanojković, J., Todorović, S., Pećinar, I., Lević, S., Ćalić, S.,& Janošević, D.. (2020). Leaf glandular trichomes of micropropagated Inula britannica – Effect of sucrose on trichome density, distribution and chemical profile. in Industrial Crops and Products
Elsevier BV., 113101.
https://doi.org/10.1016/j.indcrop.2020.113101

Stanojković J, Todorović S, Pećinar I, Lević S, Ćalić S, Janošević D. Leaf glandular trichomes of micropropagated Inula britannica – Effect of sucrose on trichome density, distribution and chemical profile. in Industrial Crops and Products. 2020;:113101.
doi:10.1016/j.indcrop.2020.113101 .

Stanojković, Jelena, Todorović, Slađana, Pećinar, Ilinka, Lević, Steva, Ćalić, Snežana, Janošević, Dušica, "Leaf glandular trichomes of micropropagated Inula britannica – Effect of sucrose on trichome density, distribution and chemical profile" in Industrial Crops and Products (2020):113101,
https://doi.org/10.1016/j.indcrop.2020.113101 . .

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Janošević, Dušica
AU  - Savić, Jelena
AU  - Todorović, Slađana
AU  - Banjac, Nevena
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3774
AB  - Gibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 µM α-naphthaleneacetic acid (NAA) and 0–10 µM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1 and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1 and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expression levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.
PB  - Springer
T2  - Plant Cell, Tissue and Organ Culture
T1  - Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach
VL  - 142
DO  - 10.1007/s11240-020-01878-3
SP  - 537
EP  - 548
ER  - 

@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Janošević, Dušica and Savić, Jelena and Todorović, Slađana and Banjac, Nevena and Milojević, Jelena",
year = "2020",
abstract = "Gibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 µM α-naphthaleneacetic acid (NAA) and 0–10 µM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1 and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1 and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expression levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.",
publisher = "Springer",
journal = "Plant Cell, Tissue and Organ Culture",
title = "Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach",
volume = "142",
doi = "10.1007/s11240-020-01878-3",
pages = "537-548"
}

Belić, M., Zdravković-Korać, S., Janošević, D., Savić, J., Todorović, S., Banjac, N.,& Milojević, J.. (2020). Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach. in Plant Cell, Tissue and Organ Culture
Springer., 142, 537-548.
https://doi.org/10.1007/s11240-020-01878-3

Belić M, Zdravković-Korać S, Janošević D, Savić J, Todorović S, Banjac N, Milojević J. Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach. in Plant Cell, Tissue and Organ Culture. 2020;142:537-548.
doi:10.1007/s11240-020-01878-3 .

Belić, Maja, Zdravković-Korać, Snežana, Janošević, Dušica, Savić, Jelena, Todorović, Slađana, Banjac, Nevena, Milojević, Jelena, "Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach" in Plant Cell, Tissue and Organ Culture, 142 (2020):537-548,
https://doi.org/10.1007/s11240-020-01878-3 . .

TY  - JOUR
AU  - Cvetić Antić, Tijana
AU  - Janošević, Dušica
AU  - Maksimović, Vuk M.
AU  - Živić, Miroslav
AU  - Budimir, Snežana
AU  - Glamočlija, Jasmina
AU  - Mitrović, Aleksandra Lj
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3730
AB  - We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
PB  - NLM (Medline)
T2  - Journal of Plant Physiology
T1  - Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
VL  - 244
DO  - 10.1016/j.jplph.2019.153086
SP  - 153086
ER  - 

@article{
author = "Cvetić Antić, Tijana and Janošević, Dušica and Maksimović, Vuk M. and Živić, Miroslav and Budimir, Snežana and Glamočlija, Jasmina and Mitrović, Aleksandra Lj",
year = "2020",
abstract = "We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.",
publisher = "NLM (Medline)",
journal = "Journal of Plant Physiology",
title = "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro",
volume = "244",
doi = "10.1016/j.jplph.2019.153086",
pages = "153086"
}

Cvetić Antić, T., Janošević, D., Maksimović, V. M., Živić, M., Budimir, S., Glamočlija, J.,& Mitrović, A. L.. (2020). Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology
NLM (Medline)., 244, 153086.
https://doi.org/10.1016/j.jplph.2019.153086

Cvetić Antić T, Janošević D, Maksimović VM, Živić M, Budimir S, Glamočlija J, Mitrović AL. Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology. 2020;244:153086.
doi:10.1016/j.jplph.2019.153086 .

Cvetić Antić, Tijana, Janošević, Dušica, Maksimović, Vuk M., Živić, Miroslav, Budimir, Snežana, Glamočlija, Jasmina, Mitrović, Aleksandra Lj, "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro" in Journal of Plant Physiology, 244 (2020):153086,
https://doi.org/10.1016/j.jplph.2019.153086 . .

TY  - CONF
AU  - Devrnja, Nina
AU  - Janošević, Dušica
AU  - Tešević, Vele
AU  - Savić, Jelena
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
AU  - Ćalić, Dušica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4323
AB  - The morphology and structure of tansy glandular trichomes were characterized for the first
time using scanning electron microscopy and light microscopy, and their secretion was analyzed
by histochemical staining. Leaf indumentum of tansy was covered with non-glandular and glandular trichomes of biseriate type, distributed on both adaxial and abaxial surface. Both types of
trichomes were also distributed along the stem, but with lower density. Light microscopy showed
that biseriate glandular trichomes consisted of two broad basal cells embedded in the epidermis,
a short single-celled stalk, and a large round multicellular head. The histochemical analysis of the
glandular secretion, performed through several staining methods, revealed the complex nature of
the secreted products, including lipids, terpenes, alkaloids and polysaccharides.
GC/MS phytochemical analysis of volatile compounds secreted by glandular trichomes, identified 42 compounds, representing >97% of the total volatiles in EO, obtained by hydrodistillation
in a Clevenger-type apparatus. The monoterpene fraction was dominant, with the prevalence of
oxygenated monoterpenes (38.5%), while the majority of sesquiterpene fraction referred to oxygenated sesquiterpenes (22.6%). Two dominant compounds, which determined the essential oil
chemotype, were trans-thujone (22.7%) and neryl-isovalerate (20.6%).
The essential oil protects the aerial parts of the plant against herbivores and pathogens and the
biological activity of the secreted products indicates their potential as pesticides or pharmaceuticals.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4323
ER  - 

@conference{
author = "Devrnja, Nina and Janošević, Dušica and Tešević, Vele and Savić, Jelena and Zdravković-Korać, Snežana and Milojević, Jelena and Ćalić, Dušica",
year = "2018",
abstract = "The morphology and structure of tansy glandular trichomes were characterized for the first
time using scanning electron microscopy and light microscopy, and their secretion was analyzed
by histochemical staining. Leaf indumentum of tansy was covered with non-glandular and glandular trichomes of biseriate type, distributed on both adaxial and abaxial surface. Both types of
trichomes were also distributed along the stem, but with lower density. Light microscopy showed
that biseriate glandular trichomes consisted of two broad basal cells embedded in the epidermis,
a short single-celled stalk, and a large round multicellular head. The histochemical analysis of the
glandular secretion, performed through several staining methods, revealed the complex nature of
the secreted products, including lipids, terpenes, alkaloids and polysaccharides.
GC/MS phytochemical analysis of volatile compounds secreted by glandular trichomes, identified 42 compounds, representing >97% of the total volatiles in EO, obtained by hydrodistillation
in a Clevenger-type apparatus. The monoterpene fraction was dominant, with the prevalence of
oxygenated monoterpenes (38.5%), while the majority of sesquiterpene fraction referred to oxygenated sesquiterpenes (22.6%). Two dominant compounds, which determined the essential oil
chemotype, were trans-thujone (22.7%) and neryl-isovalerate (20.6%).
The essential oil protects the aerial parts of the plant against herbivores and pathogens and the
biological activity of the secreted products indicates their potential as pesticides or pharmaceuticals.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4323"
}

Devrnja, N., Janošević, D., Tešević, V., Savić, J., Zdravković-Korać, S., Milojević, J.,& Ćalić, D.. (2018). Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 36.
https://hdl.handle.net/21.15107/rcub_ibiss_4323

Devrnja N, Janošević D, Tešević V, Savić J, Zdravković-Korać S, Milojević J, Ćalić D. Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:36.
https://hdl.handle.net/21.15107/rcub_ibiss_4323 .

Devrnja, Nina, Janošević, Dušica, Tešević, Vele, Savić, Jelena, Zdravković-Korać, Snežana, Milojević, Jelena, Ćalić, Dušica, "Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):36,
https://hdl.handle.net/21.15107/rcub_ibiss_4323 .

TY  - CHAP
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Budimir, Snežana
PY  - 2018
UR  - http://link.springer.com/10.1007/978-1-4939-7672-0_21
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3000
AB  - Leaf senescence constitutes a highly regulated final phase of leaf development, leading to cell death that is recognized as a type of programmed cell death (PCD). Degradation of nuclear DNA into oligonucleosomal fragments (DNA ladder) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay are methods commonly used to detect PCD-specific DNA cleavage. TUNEL reaction in situ labels free 3′-OH DNA strand breaks (nicks), thus allowing histological localization of nuclear DNA degradation during PCD. Here we describe in situ labeling of PCD-specific nuclear DNA fragmentation on conventional histological sections of senescing tobacco leaves. Incorporation of fluorescein-labeled dUTPs is detected by fluorescence microscopy, which enables in situ visualization of PCD at the single-cell level in the leaf mesophyll tissues undergoing senescence.
PB  - Humana Press, New York, NY
T2  - Plant Senescence: Methods and Protocols
T2  - Plant Senescence: Methods and Protocols
T1  - In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay
DO  - 10.1007/978-1-4939-7672-0_21
SP  - 267
EP  - 282
ER  - 

@inbook{
author = "Uzelac, Branka and Janošević, Dušica and Budimir, Snežana",
year = "2018",
abstract = "Leaf senescence constitutes a highly regulated final phase of leaf development, leading to cell death that is recognized as a type of programmed cell death (PCD). Degradation of nuclear DNA into oligonucleosomal fragments (DNA ladder) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay are methods commonly used to detect PCD-specific DNA cleavage. TUNEL reaction in situ labels free 3′-OH DNA strand breaks (nicks), thus allowing histological localization of nuclear DNA degradation during PCD. Here we describe in situ labeling of PCD-specific nuclear DNA fragmentation on conventional histological sections of senescing tobacco leaves. Incorporation of fluorescein-labeled dUTPs is detected by fluorescence microscopy, which enables in situ visualization of PCD at the single-cell level in the leaf mesophyll tissues undergoing senescence.",
publisher = "Humana Press, New York, NY",
journal = "Plant Senescence: Methods and Protocols, Plant Senescence: Methods and Protocols",
booktitle = "In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay",
doi = "10.1007/978-1-4939-7672-0_21",
pages = "267-282"
}

Uzelac, B., Janošević, D.,& Budimir, S.. (2018). In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay. in Plant Senescence: Methods and Protocols
Humana Press, New York, NY., 267-282.
https://doi.org/10.1007/978-1-4939-7672-0_21

Uzelac B, Janošević D, Budimir S. In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay. in Plant Senescence: Methods and Protocols. 2018;:267-282.
doi:10.1007/978-1-4939-7672-0_21 .

Uzelac, Branka, Janošević, Dušica, Budimir, Snežana, "In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay" in Plant Senescence: Methods and Protocols (2018):267-282,
https://doi.org/10.1007/978-1-4939-7672-0_21 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2017
UR  - http://doi.wiley.com/10.1002/jemt.22864
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2681
AB  - The anatomy and ultrastructure of the short glandular trichomes occurring on young expanding leaves of Nicotiana tabacum were investigated using light and transmission electron microscopy. The objective of the present research was to characterize the cellular changes that occur during morphogenesis of short glandular trichomes, from initiation to senescence. Ultrastructural analysis of their secretory cells revealed characteristics common to gland cells: numerous mitochondria in highly organized cytoplasm, large nuclei, and an elaborate network of endoplasmic reticulum. Initial changes in nuclear and plastidial organization were observed at a more advanced secretory stage, marking the onset of senescence. During trichome senescence, gradual reduction of the cytoplasm density occurred along with structural changes of the plastids and the tonoplast. As a result of inward blebbing of the cytoplasm into the vacuole, membrane bound vesicular structures appeared in the vacuolar space. At the late secretory stage, marked by an increase in vacuolation and extraplasmic space, degenerative changes included further fragmentation of the cytoplasm and deterioration of the tonoplast. Multimembrane myelin bodies observed in the vacuolar space were indicative of membrane digestion although plasma membrane did not appear massively degraded.
T2  - Microscopy Research and Technique
T1  - Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes
DO  - 10.1002/jemt.22864
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2017",
abstract = "The anatomy and ultrastructure of the short glandular trichomes occurring on young expanding leaves of Nicotiana tabacum were investigated using light and transmission electron microscopy. The objective of the present research was to characterize the cellular changes that occur during morphogenesis of short glandular trichomes, from initiation to senescence. Ultrastructural analysis of their secretory cells revealed characteristics common to gland cells: numerous mitochondria in highly organized cytoplasm, large nuclei, and an elaborate network of endoplasmic reticulum. Initial changes in nuclear and plastidial organization were observed at a more advanced secretory stage, marking the onset of senescence. During trichome senescence, gradual reduction of the cytoplasm density occurred along with structural changes of the plastids and the tonoplast. As a result of inward blebbing of the cytoplasm into the vacuole, membrane bound vesicular structures appeared in the vacuolar space. At the late secretory stage, marked by an increase in vacuolation and extraplasmic space, degenerative changes included further fragmentation of the cytoplasm and deterioration of the tonoplast. Multimembrane myelin bodies observed in the vacuolar space were indicative of membrane digestion although plasma membrane did not appear massively degraded.",
journal = "Microscopy Research and Technique",
title = "Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes",
doi = "10.1002/jemt.22864"
}

Uzelac, B., Janošević, D., Stojičić, D.,& Budimir, S.. (2017). Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes. in Microscopy Research and Technique.
https://doi.org/10.1002/jemt.22864

Uzelac B, Janošević D, Stojičić D, Budimir S. Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes. in Microscopy Research and Technique. 2017;.
doi:10.1002/jemt.22864 .

Uzelac, Branka, Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes" in Microscopy Research and Technique (2017),
https://doi.org/10.1002/jemt.22864 . .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6200
AB  - The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.
PB  - Berlin Heidelberg : Springer-Verlag
T2  - Planta
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)
IS  - 2
VL  - 244
DO  - 10.1007/s00425-016-2513-7
SP  - 393
EP  - 404
ER  - 

@article{
author = "Stojičić, Dragana and Tošić, Svetlana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Janošević, Dušica and Uzelac, Branka",
year = "2016",
abstract = "The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.",
publisher = "Berlin Heidelberg : Springer-Verlag",
journal = "Planta",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)",
number = "2",
volume = "244",
doi = "10.1007/s00425-016-2513-7",
pages = "393-404"
}

Stojičić, D., Tošić, S., Slavkovska, V., Zlatković, B., Budimir, S., Janošević, D.,& Uzelac, B.. (2016). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta
Berlin Heidelberg : Springer-Verlag., 244(2), 393-404.
https://doi.org/10.1007/s00425-016-2513-7

Stojičić D, Tošić S, Slavkovska V, Zlatković B, Budimir S, Janošević D, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta. 2016;244(2):393-404.
doi:10.1007/s00425-016-2513-7 .

Stojičić, Dragana, Tošić, Svetlana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Janošević, Dušica, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)" in Planta, 244, no. 2 (2016):393-404,
https://doi.org/10.1007/s00425-016-2513-7 . .

TY  - JOUR
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Banjac, Nevena
AU  - Milojević, Jelena
AU  - Janošević, Dušica
AU  - Budimir, Snežana
AU  - Zdravković-Korać, Snežana
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4358
AB  - Unlike garlic and onion, the regeneration of chive (Allium schoenoprasum L.), cultivated both for culinary and ornamental purposes, has not been intensively studied. The effects of the eight cytokinins and the plant basal section thickness on regeneration efficiency and subsequent plant growth were studied. Representatives of all cytokinin structural groups: isoprenoide side chain (trans-zeatin) and aromatic side chain (benziladenine, kinetin, meta-topolin) adenine derivatives, and phenylurea derivatives (thidiazuron and N-(2-chloro-4-pyridyl)-N′-phenylurea) at 0, 1, 5 or 10 μM were used. Histological analysis revealed adventitious buds formation from the leaf sheaths’ bases and the basal plate. The highest regeneration frequency (100 %) and the mean bud number per explant (20.0) were achieved with 10 μM thidiazuron (TDZ), and 5 mm-thick basal sections were the most responsive explants. Inferior shoot and root growth characteristics of plants regenerated by this treatment was avoided by exclusion or replacement of 10 μM TDZ with 5 μM kinetin (Kin) after a 4-week bud induction period, without consequences on the regeneration efficiency. In addition, a positive correlation between peroxidase, catalase and superoxide dismutase activity and the regeneration capacity was observed. All antioxidative enzymes activity changed much faster with 10 μM TDZ than with 1 μM Kin, which provoked the weakest regeneration response. Moreover, a unique peroxidase isoform was observed only in TDZ-treated explants after 3rd day of treatment. This work is useful for genetic engineering and virus-free plant production advancement, and for the knowledge expansion regarding the role of antioxidative enzymes in plant organogenesis.
PB  - Dordrecht: Springer
T2  - Plant Cell Tissue and Organ Culture
T1  - Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)
IS  - 1
VL  - 124
DO  - 10.1007/s11240-015-0869-1
SP  - 1
EP  - 14
ER  - 

@article{
author = "Tubić, Ljiljana and Savić, Jelena and Banjac, Nevena and Milojević, Jelena and Janošević, Dušica and Budimir, Snežana and Zdravković-Korać, Snežana",
year = "2016",
abstract = "Unlike garlic and onion, the regeneration of chive (Allium schoenoprasum L.), cultivated both for culinary and ornamental purposes, has not been intensively studied. The effects of the eight cytokinins and the plant basal section thickness on regeneration efficiency and subsequent plant growth were studied. Representatives of all cytokinin structural groups: isoprenoide side chain (trans-zeatin) and aromatic side chain (benziladenine, kinetin, meta-topolin) adenine derivatives, and phenylurea derivatives (thidiazuron and N-(2-chloro-4-pyridyl)-N′-phenylurea) at 0, 1, 5 or 10 μM were used. Histological analysis revealed adventitious buds formation from the leaf sheaths’ bases and the basal plate. The highest regeneration frequency (100 %) and the mean bud number per explant (20.0) were achieved with 10 μM thidiazuron (TDZ), and 5 mm-thick basal sections were the most responsive explants. Inferior shoot and root growth characteristics of plants regenerated by this treatment was avoided by exclusion or replacement of 10 μM TDZ with 5 μM kinetin (Kin) after a 4-week bud induction period, without consequences on the regeneration efficiency. In addition, a positive correlation between peroxidase, catalase and superoxide dismutase activity and the regeneration capacity was observed. All antioxidative enzymes activity changed much faster with 10 μM TDZ than with 1 μM Kin, which provoked the weakest regeneration response. Moreover, a unique peroxidase isoform was observed only in TDZ-treated explants after 3rd day of treatment. This work is useful for genetic engineering and virus-free plant production advancement, and for the knowledge expansion regarding the role of antioxidative enzymes in plant organogenesis.",
publisher = "Dordrecht: Springer",
journal = "Plant Cell Tissue and Organ Culture",
title = "Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)",
number = "1",
volume = "124",
doi = "10.1007/s11240-015-0869-1",
pages = "1-14"
}

Tubić, L., Savić, J., Banjac, N., Milojević, J., Janošević, D., Budimir, S.,& Zdravković-Korać, S.. (2016). Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.). in Plant Cell Tissue and Organ Culture
Dordrecht: Springer., 124(1), 1-14.
https://doi.org/10.1007/s11240-015-0869-1

Tubić L, Savić J, Banjac N, Milojević J, Janošević D, Budimir S, Zdravković-Korać S. Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.). in Plant Cell Tissue and Organ Culture. 2016;124(1):1-14.
doi:10.1007/s11240-015-0869-1 .

Tubić, Ljiljana, Savić, Jelena, Banjac, Nevena, Milojević, Jelena, Janošević, Dušica, Budimir, Snežana, Zdravković-Korać, Snežana, "Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)" in Plant Cell Tissue and Organ Culture, 124, no. 1 (2016):1-14,
https://doi.org/10.1007/s11240-015-0869-1 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Simonović, Ana
AU  - Motyka, Václav
AU  - Dobrev, Petre I
AU  - Budimir, Snežana
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2989
UR  - https://link.springer.com/article/10.1007%2Fs00709-015-0802-9#aboutcontent
AB  - Leaf senescence is a highly regulated final phase of leaf development preceding massive cell death. It results in the coordinated degradation of macromolecules and the subsequent nutrient relocation to other plant parts. Very little is still known about early stages of leaf senescence during normal leaf ontogeny that is not triggered by stress factors. This paper comprises an integrated study of natural leaf senescence in tobacco plants grown in vitro, using molecular, structural, and physiological information. We determined the time sequence of ultrastructural changes in mesophyll cells during leaf senescence, showing that the degradation of chloroplast ultrastructure fully correlated with changes in chlorophyll content. The earliest degenerative changes in chloroplast ultrastructure coinciding with early chromatin condensation were observed already in mature green leaves. A continuum of degradative changes in chloroplast ultrastructure, chromatin condensation and aggregation, along with progressive decrease in cytoplasm organization and electron density were observed in the course of mesophyll cells ageing. Although the total amounts of endogenous cytokinins gradually increased during leaf ontogenesis, the proportion of bioactive cytokinin forms, as well as their phosphate precursors, in total cytokinin content rapidly declined with ageing. Endogenous indole-3-acetic acid (IAA) levels were strongly reduced in senescent leaves, and a decreasing tendency was also observed for abscisic acid (ABA) levels. Senescence-associated tobacco cysteine proteases (CP, E.C. 3.4.22) CP1 and CP23 genes were induced in the initial phase of senescence. Genes encoding glutamate dehydrogenase (GDH, E.C. 1.4.1.2) and one isoform of cytosolic glutamine synthetase (GS1, E.C. 6.3.1.2) were induced in the late stage of senescence, while chloroplastic GS (GS2) gene showed a continuous decrease with leaf ageing.
PB  - Springer-Verlag Wien
T2  - Protoplasma
T1  - Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.
IS  - 2
VL  - 253
DO  - 10.1007/s00709-015-0802-9
SP  - 259
EP  - 75
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Simonović, Ana and Motyka, Václav and Dobrev, Petre I and Budimir, Snežana",
year = "2016",
abstract = "Leaf senescence is a highly regulated final phase of leaf development preceding massive cell death. It results in the coordinated degradation of macromolecules and the subsequent nutrient relocation to other plant parts. Very little is still known about early stages of leaf senescence during normal leaf ontogeny that is not triggered by stress factors. This paper comprises an integrated study of natural leaf senescence in tobacco plants grown in vitro, using molecular, structural, and physiological information. We determined the time sequence of ultrastructural changes in mesophyll cells during leaf senescence, showing that the degradation of chloroplast ultrastructure fully correlated with changes in chlorophyll content. The earliest degenerative changes in chloroplast ultrastructure coinciding with early chromatin condensation were observed already in mature green leaves. A continuum of degradative changes in chloroplast ultrastructure, chromatin condensation and aggregation, along with progressive decrease in cytoplasm organization and electron density were observed in the course of mesophyll cells ageing. Although the total amounts of endogenous cytokinins gradually increased during leaf ontogenesis, the proportion of bioactive cytokinin forms, as well as their phosphate precursors, in total cytokinin content rapidly declined with ageing. Endogenous indole-3-acetic acid (IAA) levels were strongly reduced in senescent leaves, and a decreasing tendency was also observed for abscisic acid (ABA) levels. Senescence-associated tobacco cysteine proteases (CP, E.C. 3.4.22) CP1 and CP23 genes were induced in the initial phase of senescence. Genes encoding glutamate dehydrogenase (GDH, E.C. 1.4.1.2) and one isoform of cytosolic glutamine synthetase (GS1, E.C. 6.3.1.2) were induced in the late stage of senescence, while chloroplastic GS (GS2) gene showed a continuous decrease with leaf ageing.",
publisher = "Springer-Verlag Wien",
journal = "Protoplasma",
title = "Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.",
number = "2",
volume = "253",
doi = "10.1007/s00709-015-0802-9",
pages = "259-75"
}

Uzelac, B., Janošević, D., Simonović, A., Motyka, V., Dobrev, P. I.,& Budimir, S.. (2016). Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.. in Protoplasma
Springer-Verlag Wien., 253(2), 259-75.
https://doi.org/10.1007/s00709-015-0802-9

Uzelac B, Janošević D, Simonović A, Motyka V, Dobrev PI, Budimir S. Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.. in Protoplasma. 2016;253(2):259-75.
doi:10.1007/s00709-015-0802-9 .

Uzelac, Branka, Janošević, Dušica, Simonović, Ana, Motyka, Václav, Dobrev, Petre I, Budimir, Snežana, "Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro." in Protoplasma, 253, no. 2 (2016):259-75,
https://doi.org/10.1007/s00709-015-0802-9 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2015
UR  - https://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2015_39_2_632_full.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4063
AB  - The tall glandular trichomes occurring on young expanding leaves of Nicotiana tabacum grown in
vitro were investigated using light and electron microscopy. Previous studies reported that the tall
glandular trichomes of tobacco produce large quantities of various compounds including diterpenes.
To explore the cellular structures required and putatively involved in diterpene biosynthesis and
secretion, the present study focused on the development of the tall glandular trichomes, and on
histochemical and ultrastructural analysis of their secretory cells under in vitro conditions. Electron
microscopy confirmed earlier light microscopy observations of the glandular nature of the tall leaf
trichomes. Their head cells, which secrete resinous material, exhibited characteristics common
to gland cells: a dense cytoplasm, numerous mitochondria and little vacuolation. They contained
structurally well-developed chloroplasts and an elaborate network of endoplasmic reticulum, as
well as electron dense inclusions in chloroplasts and in small vacuoles. The dominance of smooth
endoplasmic reticulum suggests its involvement in diterpene biosynthesis. Histochemical tests were
positive for both lipophilic and hydrophilic secretions, which included terpenoids (essential oils
and resins) and acidic/neutral lipids, as well as phenolic compounds (tannins), alkaloids, mucilage/
pectin and polysaccharides.
PB  - Belgrade: Institute of Botany and Botanical Garden "Jevremovac"
T2  - Botanica Serbica
T1  - In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes
IS  - 2
VL  - 39
SP  - 103
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4063
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2015",
abstract = "The tall glandular trichomes occurring on young expanding leaves of Nicotiana tabacum grown in
vitro were investigated using light and electron microscopy. Previous studies reported that the tall
glandular trichomes of tobacco produce large quantities of various compounds including diterpenes.
To explore the cellular structures required and putatively involved in diterpene biosynthesis and
secretion, the present study focused on the development of the tall glandular trichomes, and on
histochemical and ultrastructural analysis of their secretory cells under in vitro conditions. Electron
microscopy confirmed earlier light microscopy observations of the glandular nature of the tall leaf
trichomes. Their head cells, which secrete resinous material, exhibited characteristics common
to gland cells: a dense cytoplasm, numerous mitochondria and little vacuolation. They contained
structurally well-developed chloroplasts and an elaborate network of endoplasmic reticulum, as
well as electron dense inclusions in chloroplasts and in small vacuoles. The dominance of smooth
endoplasmic reticulum suggests its involvement in diterpene biosynthesis. Histochemical tests were
positive for both lipophilic and hydrophilic secretions, which included terpenoids (essential oils
and resins) and acidic/neutral lipids, as well as phenolic compounds (tannins), alkaloids, mucilage/
pectin and polysaccharides.",
publisher = "Belgrade: Institute of Botany and Botanical Garden "Jevremovac"",
journal = "Botanica Serbica",
title = "In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes",
number = "2",
volume = "39",
pages = "103-110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4063"
}

Uzelac, B., Janošević, D., Stojičić, D.,& Budimir, S.. (2015). In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden "Jevremovac"., 39(2), 103-110.
https://hdl.handle.net/21.15107/rcub_ibiss_4063

Uzelac B, Janošević D, Stojičić D, Budimir S. In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes. in Botanica Serbica. 2015;39(2):103-110.
https://hdl.handle.net/21.15107/rcub_ibiss_4063 .

Uzelac, Branka, Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes" in Botanica Serbica, 39, no. 2 (2015):103-110,
https://hdl.handle.net/21.15107/rcub_ibiss_4063 .

TY  - JOUR
AU  - Al Sheef Najat, Beleed
AU  - Duletić-Laušević, Sonja N.
AU  - Janošević, Dušica
AU  - Budimir, Snežana
AU  - Marin, Marija
AU  - Alimpić, Ana
AU  - Giweli, A.A.M.
AU  - Marin, P.D.
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/327
AB  - Micromorphological and ultrastructural analyses of the leaf trichomes of Salvia fruticosa Mill. were performed by light and electron microscopy. The leaves bear numerous non-glandular unbranched trichomes, and peltate, capitate and digitiform glandular trichomes. Very elongated flagelliform non-glandular trichomes densely covered the leaf surfaces, with especially abundance on the leaf margins. Peltate trichomes consist of a basal epidermal cell, a very short stalk cell and a large round head of eight secretory cells arranged in a circle. Capitate trichomes can be divided into two main types, short-stalked and long-stalked, and further into five subtypes according to the number of stalk cells, morphology and number of glandular head cells. Digitiform trichomes consist of one basal cell, one or two stalk cells and one apical secretory cell, which are of similar diameter and approximately equal length.
T2  - Archives of Biological Sciences
T1  - Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill.
IS  - 1
VL  - 65
SP  - 239
EP  - 246
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_327
ER  - 

@article{
author = "Al Sheef Najat, Beleed and Duletić-Laušević, Sonja N. and Janošević, Dušica and Budimir, Snežana and Marin, Marija and Alimpić, Ana and Giweli, A.A.M. and Marin, P.D.",
year = "2013, 2013",
abstract = "Micromorphological and ultrastructural analyses of the leaf trichomes of Salvia fruticosa Mill. were performed by light and electron microscopy. The leaves bear numerous non-glandular unbranched trichomes, and peltate, capitate and digitiform glandular trichomes. Very elongated flagelliform non-glandular trichomes densely covered the leaf surfaces, with especially abundance on the leaf margins. Peltate trichomes consist of a basal epidermal cell, a very short stalk cell and a large round head of eight secretory cells arranged in a circle. Capitate trichomes can be divided into two main types, short-stalked and long-stalked, and further into five subtypes according to the number of stalk cells, morphology and number of glandular head cells. Digitiform trichomes consist of one basal cell, one or two stalk cells and one apical secretory cell, which are of similar diameter and approximately equal length.",
journal = "Archives of Biological Sciences",
title = "Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill.",
number = "1",
volume = "65",
pages = "239-246",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_327"
}

Al Sheef Najat, B., Duletić-Laušević, S. N., Janošević, D., Budimir, S., Marin, M., Alimpić, A., Giweli, A.A.M.,& Marin, P.D.. (2013). Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill.. in Archives of Biological Sciences, 65(1), 239-246.
https://hdl.handle.net/21.15107/rcub_ibiss_327

Al Sheef Najat B, Duletić-Laušević SN, Janošević D, Budimir S, Marin M, Alimpić A, Giweli A, Marin P. Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill.. in Archives of Biological Sciences. 2013;65(1):239-246.
https://hdl.handle.net/21.15107/rcub_ibiss_327 .

Al Sheef Najat, Beleed, Duletić-Laušević, Sonja N., Janošević, Dušica, Budimir, Snežana, Marin, Marija, Alimpić, Ana, Giweli, A.A.M., Marin, P.D., "Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill." in Archives of Biological Sciences, 65, no. 1 (2013):239-246,
https://hdl.handle.net/21.15107/rcub_ibiss_327 .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/308
AB  - Cytokinins are involved in plant cell proliferation leading to plant growth and morphogenesis. The size, activity and maintenance of the shoot apical meristem (SAM) are defined by a balanced rate of mitotic cell divisions and functional cell differentiation that are controlled by cytokinins. In order to investigate the effect of exogenous cytokinin on SAM, morpho-anatomical changes in the shoot apices of tobacco treated with benzyladenine (BA) were compared to those of untreated control plants.
T2  - Archives of Biological Sciences
T1  - Effect of cytokinins on shoot apical meristem in Nicotiana tabacum
IS  - 2
VL  - 64
SP  - 511
EP  - 516
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_308
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2012, 2012",
abstract = "Cytokinins are involved in plant cell proliferation leading to plant growth and morphogenesis. The size, activity and maintenance of the shoot apical meristem (SAM) are defined by a balanced rate of mitotic cell divisions and functional cell differentiation that are controlled by cytokinins. In order to investigate the effect of exogenous cytokinin on SAM, morpho-anatomical changes in the shoot apices of tobacco treated with benzyladenine (BA) were compared to those of untreated control plants.",
journal = "Archives of Biological Sciences",
title = "Effect of cytokinins on shoot apical meristem in Nicotiana tabacum",
number = "2",
volume = "64",
pages = "511-516",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_308"
}

Uzelac, B., Janošević, D., Stojičić, D.,& Budimir, S.. (2012). Effect of cytokinins on shoot apical meristem in Nicotiana tabacum. in Archives of Biological Sciences, 64(2), 511-516.
https://hdl.handle.net/21.15107/rcub_ibiss_308

Uzelac B, Janošević D, Stojičić D, Budimir S. Effect of cytokinins on shoot apical meristem in Nicotiana tabacum. in Archives of Biological Sciences. 2012;64(2):511-516.
https://hdl.handle.net/21.15107/rcub_ibiss_308 .

Uzelac, Branka, Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "Effect of cytokinins on shoot apical meristem in Nicotiana tabacum" in Archives of Biological Sciences, 64, no. 2 (2012):511-516,
https://hdl.handle.net/21.15107/rcub_ibiss_308 .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
AU  - Čokeša, V.
AU  - Budimir, Snežana
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/307
AB  - This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.
T2  - Archives of Biological Sciences
T1  - In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth
IS  - 2
VL  - 64
SP  - 503
EP  - 509
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_307
ER  - 

@article{
author = "Stojičić, Dragana and Janošević, Dušica and Uzelac, Branka and Čokeša, V. and Budimir, Snežana",
year = "2012, 2012",
abstract = "This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.",
journal = "Archives of Biological Sciences",
title = "In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth",
number = "2",
volume = "64",
pages = "503-509",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_307"
}

Stojičić, D., Janošević, D., Uzelac, B., Čokeša, V.,& Budimir, S.. (2012). In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth. in Archives of Biological Sciences, 64(2), 503-509.
https://hdl.handle.net/21.15107/rcub_ibiss_307

Stojičić D, Janošević D, Uzelac B, Čokeša V, Budimir S. In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth. in Archives of Biological Sciences. 2012;64(2):503-509.
https://hdl.handle.net/21.15107/rcub_ibiss_307 .

Stojičić, Dragana, Janošević, Dušica, Uzelac, Branka, Čokeša, V., Budimir, Snežana, "In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth" in Archives of Biological Sciences, 64, no. 2 (2012):503-509,
https://hdl.handle.net/21.15107/rcub_ibiss_307 .

TY  - JOUR
AU  - Ninković, Slavica
AU  - Uzelac, Branka
AU  - Nikolić, Marija
AU  - Todorović, Slađana
AU  - Janošević, Dušica
AU  - Cingel, Aleksandar
AU  - Ghalawengi, N.
AU  - Vinterhalter, D.
AU  - Budimir, Snežana
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/191
T2  - Archives of Biological Sciences
T1  - In vitro multiplication of oryzacystatin II transformed Alfalfa on GA3-containing medium
IS  - 1
VL  - 60
SP  - 9
EP  - 10
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_191
ER  - 

@article{
author = "Ninković, Slavica and Uzelac, Branka and Nikolić, Marija and Todorović, Slađana and Janošević, Dušica and Cingel, Aleksandar and Ghalawengi, N. and Vinterhalter, D. and Budimir, Snežana",
year = "2008, 2008",
journal = "Archives of Biological Sciences",
title = "In vitro multiplication of oryzacystatin II transformed Alfalfa on GA3-containing medium",
number = "1",
volume = "60",
pages = "9-10",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_191"
}

Ninković, S., Uzelac, B., Nikolić, M., Todorović, S., Janošević, D., Cingel, A., Ghalawengi, N., Vinterhalter, D.,& Budimir, S.. (2008). In vitro multiplication of oryzacystatin II transformed Alfalfa on GA3-containing medium. in Archives of Biological Sciences, 60(1), 9-10.
https://hdl.handle.net/21.15107/rcub_ibiss_191

Ninković S, Uzelac B, Nikolić M, Todorović S, Janošević D, Cingel A, Ghalawengi N, Vinterhalter D, Budimir S. In vitro multiplication of oryzacystatin II transformed Alfalfa on GA3-containing medium. in Archives of Biological Sciences. 2008;60(1):9-10.
https://hdl.handle.net/21.15107/rcub_ibiss_191 .

Ninković, Slavica, Uzelac, Branka, Nikolić, Marija, Todorović, Slađana, Janošević, Dušica, Cingel, Aleksandar, Ghalawengi, N., Vinterhalter, D., Budimir, Snežana, "In vitro multiplication of oryzacystatin II transformed Alfalfa on GA3-containing medium" in Archives of Biological Sciences, 60, no. 1 (2008):9-10,
https://hdl.handle.net/21.15107/rcub_ibiss_191 .

TY  - JOUR
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/520
AB  - The potential of somatic embryogenesis induction was examined by in vitro growing of isolated ovules of Pinus heldreichii (Christ.) on nutrient media. The induction of embryogenic tissue was stimulated by Gresshoff and Doy (GD) medium with nitrogen compounds reduced to one half and with 0.5 mg/l BA and 2.0 mg/l 2.4-D. Still, the most important factor in this process was the development stage of embryos in the ovule. An induction frequency of 10% was obtained on the ovules in which embryos were in the pre-cotyledonary stage. When the dominant embryo in the ovule was in the cotyledonary stage, the induction frequency was lower or absent. Proliferation of embryogenic tissue was achieved on the media with reduced concentration of growth regulators, and the initial stages of embryo maturation were obtained on the media with ABA and 5% sucrose.
AB  - Izolovane ovule Pinus heldreichii (Christ.) gajene su in vitro na hranljivim podlogama radi ispitivanja mogućnosti indukcije somatske embriogeneze. Hranljiva podloga Gresshoff and Doy (GD) sa azotnim jedinjenjima, smanjenim na polovinu i BA 0.5 mg/L i 2.4-D 2.0 mg/L, stimulisala je indukciju embriogenog tkiva. Ipak, najvažniji faktor u ovom procesu bio je stadijum razvića embriona u ovuli. Frekvencija indukcije od 10% dobijena je na ovulama u kojima su embrioni bili u prekotiledonarnom stadijumu razvića. Kada je dominantni embrion u ovuli bio u kotiledonarnom stadijumu razvića, frekvencija indukcije bila je manja ili je nije ni bilo. Proliferacija embriogenog tkiva dobijena je na podlogama sa smanjenom koncentracijom regulatora rastenja, a početni stadijumi sazrevanja embriona na podlogama sa ABA i 5% saharoze.
T2  - Šumarstvo
T1  - Uticaj stadijuma razvića megagametofita Pinus heldreichii na indukciju embriogenog tkiva
T1  - Effect of development stage of Pinus heldreichii megagametophytes on the induction of embryogenic tissue
IS  - 1-2
VL  - 60
SP  - 93
EP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_520
ER  - 

@article{
author = "Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2008, 2008",
abstract = "The potential of somatic embryogenesis induction was examined by in vitro growing of isolated ovules of Pinus heldreichii (Christ.) on nutrient media. The induction of embryogenic tissue was stimulated by Gresshoff and Doy (GD) medium with nitrogen compounds reduced to one half and with 0.5 mg/l BA and 2.0 mg/l 2.4-D. Still, the most important factor in this process was the development stage of embryos in the ovule. An induction frequency of 10% was obtained on the ovules in which embryos were in the pre-cotyledonary stage. When the dominant embryo in the ovule was in the cotyledonary stage, the induction frequency was lower or absent. Proliferation of embryogenic tissue was achieved on the media with reduced concentration of growth regulators, and the initial stages of embryo maturation were obtained on the media with ABA and 5% sucrose., Izolovane ovule Pinus heldreichii (Christ.) gajene su in vitro na hranljivim podlogama radi ispitivanja mogućnosti indukcije somatske embriogeneze. Hranljiva podloga Gresshoff and Doy (GD) sa azotnim jedinjenjima, smanjenim na polovinu i BA 0.5 mg/L i 2.4-D 2.0 mg/L, stimulisala je indukciju embriogenog tkiva. Ipak, najvažniji faktor u ovom procesu bio je stadijum razvića embriona u ovuli. Frekvencija indukcije od 10% dobijena je na ovulama u kojima su embrioni bili u prekotiledonarnom stadijumu razvića. Kada je dominantni embrion u ovuli bio u kotiledonarnom stadijumu razvića, frekvencija indukcije bila je manja ili je nije ni bilo. Proliferacija embriogenog tkiva dobijena je na podlogama sa smanjenom koncentracijom regulatora rastenja, a početni stadijumi sazrevanja embriona na podlogama sa ABA i 5% saharoze.",
journal = "Šumarstvo",
title = "Uticaj stadijuma razvića megagametofita Pinus heldreichii na indukciju embriogenog tkiva, Effect of development stage of Pinus heldreichii megagametophytes on the induction of embryogenic tissue",
number = "1-2",
volume = "60",
pages = "93-101",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_520"
}

Janošević, D., Stojičić, D.,& Budimir, S.. (2008). Uticaj stadijuma razvića megagametofita Pinus heldreichii na indukciju embriogenog tkiva. in Šumarstvo, 60(1-2), 93-101.
https://hdl.handle.net/21.15107/rcub_ibiss_520

Janošević D, Stojičić D, Budimir S. Uticaj stadijuma razvića megagametofita Pinus heldreichii na indukciju embriogenog tkiva. in Šumarstvo. 2008;60(1-2):93-101.
https://hdl.handle.net/21.15107/rcub_ibiss_520 .

Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "Uticaj stadijuma razvića megagametofita Pinus heldreichii na indukciju embriogenog tkiva" in Šumarstvo, 60, no. 1-2 (2008):93-101,
https://hdl.handle.net/21.15107/rcub_ibiss_520 .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
AU  - Budimir, Snežana
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/214
AB  - The effects of nutritional, hormonal, and physical factors on the germination and growth of isolated mature zygotic embryos of Pinus heldreichii were studied under in vitro conditions. The optimum medium for embryo germination and adequate conversion into seedlings was basal Gresshoff and Doy (1GD) medium. All tested carbohydrates at the applied concentrations stimulated embryo growth, but only 3% sucrose increased embryo germination compared to the control, while maltose had an inhibitory effect. Among the applied plant growth regulators, only gibberelic acid (GA3) had a few benefits on the development of isolated embryos into plantlets, while the others had a negative effect. Embryos cultured on an agar-solidified medium grew better and the germination percentage was higher than in a liquid medium. Light treatment did not affect embryo germination, but generally stimulated seedling growth.
AB  - U kulturi in vitro ispitivan je efekat nutritivnih, hormonalnih i fizičkih faktora na klijanje i rastenje izolovanih zigotskih embriona munike (Pinus heldrechii). Većina izolovanih embriona munike klijala je već tokom prve nedelje nakon prenošenja na hranljivu podlogu. Mada embrioni mogu da klijaju i na podlozi bez soli, vitamina i ugljenih hidrata, najveći procenat klijanja i najbolje morfološke karakteristike klijanaca postignute su na bazalnoj Gresshoff & Doy podlozi (1GD) sa 3% saharozom. Od ispitivanih regulatora rastenja samo je giberelna kiselina (GA3) pozitivno delovala na razviće embriona, dok su auksini i citokinini inhibirali njihovo dalje razviće. U odnosu na embrione koji su rasli u tečnoj podlozi, embrioni sa čvrste agarne podloge, klijali su u većem procentu i dalje se pravilno razvijali. Mada generalno stimulišu rastenje klijanaca, svetlosni tretmani nisu imali efekat na klijanje embriona. Dobijeni rezultati pokazuju da se in vitro sistem može koristiti za brzu i sinhronu produkciju zdravih klijanaca elitnih genotipova koji se odlikuju niskom produkcijom semena.
T2  - Archives of Biological Sciences
T1  - Klijanje i rastenje izolovanih embriona Pinus heldreichii u kulturi in vitro
T1  - Factors influencing germination and growth of isolated embryos of Pinus heldreichii
IS  - 4
VL  - 60
SP  - 673
EP  - 679
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_214
ER  - 

@article{
author = "Stojičić, Dragana and Janošević, Dušica and Uzelac, Branka and Budimir, Snežana",
year = "2008, 2008",
abstract = "The effects of nutritional, hormonal, and physical factors on the germination and growth of isolated mature zygotic embryos of Pinus heldreichii were studied under in vitro conditions. The optimum medium for embryo germination and adequate conversion into seedlings was basal Gresshoff and Doy (1GD) medium. All tested carbohydrates at the applied concentrations stimulated embryo growth, but only 3% sucrose increased embryo germination compared to the control, while maltose had an inhibitory effect. Among the applied plant growth regulators, only gibberelic acid (GA3) had a few benefits on the development of isolated embryos into plantlets, while the others had a negative effect. Embryos cultured on an agar-solidified medium grew better and the germination percentage was higher than in a liquid medium. Light treatment did not affect embryo germination, but generally stimulated seedling growth., U kulturi in vitro ispitivan je efekat nutritivnih, hormonalnih i fizičkih faktora na klijanje i rastenje izolovanih zigotskih embriona munike (Pinus heldrechii). Većina izolovanih embriona munike klijala je već tokom prve nedelje nakon prenošenja na hranljivu podlogu. Mada embrioni mogu da klijaju i na podlozi bez soli, vitamina i ugljenih hidrata, najveći procenat klijanja i najbolje morfološke karakteristike klijanaca postignute su na bazalnoj Gresshoff & Doy podlozi (1GD) sa 3% saharozom. Od ispitivanih regulatora rastenja samo je giberelna kiselina (GA3) pozitivno delovala na razviće embriona, dok su auksini i citokinini inhibirali njihovo dalje razviće. U odnosu na embrione koji su rasli u tečnoj podlozi, embrioni sa čvrste agarne podloge, klijali su u većem procentu i dalje se pravilno razvijali. Mada generalno stimulišu rastenje klijanaca, svetlosni tretmani nisu imali efekat na klijanje embriona. Dobijeni rezultati pokazuju da se in vitro sistem može koristiti za brzu i sinhronu produkciju zdravih klijanaca elitnih genotipova koji se odlikuju niskom produkcijom semena.",
journal = "Archives of Biological Sciences",
title = "Klijanje i rastenje izolovanih embriona Pinus heldreichii u kulturi in vitro, Factors influencing germination and growth of isolated embryos of Pinus heldreichii",
number = "4",
volume = "60",
pages = "673-679",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_214"
}

Stojičić, D., Janošević, D., Uzelac, B.,& Budimir, S.. (2008). Klijanje i rastenje izolovanih embriona Pinus heldreichii u kulturi in vitro. in Archives of Biological Sciences, 60(4), 673-679.
https://hdl.handle.net/21.15107/rcub_ibiss_214

Stojičić D, Janošević D, Uzelac B, Budimir S. Klijanje i rastenje izolovanih embriona Pinus heldreichii u kulturi in vitro. in Archives of Biological Sciences. 2008;60(4):673-679.
https://hdl.handle.net/21.15107/rcub_ibiss_214 .

Stojičić, Dragana, Janošević, Dušica, Uzelac, Branka, Budimir, Snežana, "Klijanje i rastenje izolovanih embriona Pinus heldreichii u kulturi in vitro" in Archives of Biological Sciences, 60, no. 4 (2008):673-679,
https://hdl.handle.net/21.15107/rcub_ibiss_214 .

TY  - JOUR
AU  - Marin, Marija
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Marin, P.D.
AU  - Duletić-Laušević, Sonja N.
AU  - Ljaljević-Grbić, Milica
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/213
AB  - Micromorphology, distribution, and histochemistry of the trichomes of Thymus lykae were studied using scanning electron microscopy (SEM)and confocal laser scanning electron microscopy (CLSM). The leaves, stem, and calyx bear numerous glandular and non-glandular trichomes. Two types of glandular trichomes are identified - peltate and capitate. Results of histochemical tests showed positive reactions to polysaccharides, proteins, and lipids. Yellow autofluorescence of secreted material was noticed in peltate and capitate trichomes. Strong reddish-yellow autofluorescence of the lipophilic and hydrophilic secreted material was observed with CLSM.
AB  - Mikromorfologija, distribucija i histohemijska analiza trihoma kod vrste Thymus lykae urađena je korišćenjem skening elektronskog mikroskopa i konfokalnog laserskog skening mikroskopa. Nežlezdane, negranate i dva tipa žlezdanih trihoma - peltatne i kapitatne opisane suuovom radu. Brojne nežlezdane i žlezdane trihome nalazile suse kako na listovima (i na licu i na naličju)tako i na stablu i na čašici. Rezultati histohemijskih istraživanja pokazala supozitivne reakcije na polisaharide, lipide i proteine. Sekretorni produkti unutar peltatnih trihoma imali sujako žutu autofluorescenciju, dok je u kapitatnim trihomama zapažena slabija autofluorescencija. Korišćenjem CLSM uočena je jarko crveno-žuta autofluorescencija lipofilnih i hidrofilnih sekretornih materija u žlezdanim trihomama.
T2  - Archives of Biological Sciences
T1  - Morfologija, distribucija i histohemija trihoma Thymus lykae Degen et Jav. (Lamiaceae)
T1  - Morphology, distribution, and histochemistry of trichomes of Thymus lykae Degen & Jav. (Lamiaceae)
IS  - 4
VL  - 60
SP  - 667
EP  - 672
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_213
ER  - 

@article{
author = "Marin, Marija and Budimir, Snežana and Janošević, Dušica and Marin, P.D. and Duletić-Laušević, Sonja N. and Ljaljević-Grbić, Milica",
year = "2008, 2008",
abstract = "Micromorphology, distribution, and histochemistry of the trichomes of Thymus lykae were studied using scanning electron microscopy (SEM)and confocal laser scanning electron microscopy (CLSM). The leaves, stem, and calyx bear numerous glandular and non-glandular trichomes. Two types of glandular trichomes are identified - peltate and capitate. Results of histochemical tests showed positive reactions to polysaccharides, proteins, and lipids. Yellow autofluorescence of secreted material was noticed in peltate and capitate trichomes. Strong reddish-yellow autofluorescence of the lipophilic and hydrophilic secreted material was observed with CLSM., Mikromorfologija, distribucija i histohemijska analiza trihoma kod vrste Thymus lykae urađena je korišćenjem skening elektronskog mikroskopa i konfokalnog laserskog skening mikroskopa. Nežlezdane, negranate i dva tipa žlezdanih trihoma - peltatne i kapitatne opisane suuovom radu. Brojne nežlezdane i žlezdane trihome nalazile suse kako na listovima (i na licu i na naličju)tako i na stablu i na čašici. Rezultati histohemijskih istraživanja pokazala supozitivne reakcije na polisaharide, lipide i proteine. Sekretorni produkti unutar peltatnih trihoma imali sujako žutu autofluorescenciju, dok je u kapitatnim trihomama zapažena slabija autofluorescencija. Korišćenjem CLSM uočena je jarko crveno-žuta autofluorescencija lipofilnih i hidrofilnih sekretornih materija u žlezdanim trihomama.",
journal = "Archives of Biological Sciences",
title = "Morfologija, distribucija i histohemija trihoma Thymus lykae Degen et Jav. (Lamiaceae), Morphology, distribution, and histochemistry of trichomes of Thymus lykae Degen & Jav. (Lamiaceae)",
number = "4",
volume = "60",
pages = "667-672",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_213"
}

Marin, M., Budimir, S., Janošević, D., Marin, P.D., Duletić-Laušević, S. N.,& Ljaljević-Grbić, M.. (2008). Morfologija, distribucija i histohemija trihoma Thymus lykae Degen et Jav. (Lamiaceae). in Archives of Biological Sciences, 60(4), 667-672.
https://hdl.handle.net/21.15107/rcub_ibiss_213

Marin M, Budimir S, Janošević D, Marin P, Duletić-Laušević SN, Ljaljević-Grbić M. Morfologija, distribucija i histohemija trihoma Thymus lykae Degen et Jav. (Lamiaceae). in Archives of Biological Sciences. 2008;60(4):667-672.
https://hdl.handle.net/21.15107/rcub_ibiss_213 .

Marin, Marija, Budimir, Snežana, Janošević, Dušica, Marin, P.D., Duletić-Laušević, Sonja N., Ljaljević-Grbić, Milica, "Morfologija, distribucija i histohemija trihoma Thymus lykae Degen et Jav. (Lamiaceae)" in Archives of Biological Sciences, 60, no. 4 (2008):667-672,
https://hdl.handle.net/21.15107/rcub_ibiss_213 .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Ćulafić, Ljubinka
AU  - Budimir, Snežana
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/159
AB  - The potential for somatic embryogenesis in zygotic embryo and megagametophyte cultures of Pinus heldreichii was examined. Somatic embryogenesis was initiated from megagametophytes containing immature zygotic embryos at early stages of development. An induction frequency of up to 6.7% was obtained on Gresshoff and Doy medium in the presence of 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l benzyladenine (BA). Formation and further proliferation of embryogenic tissue were achieved upon transfer of explants to a medium with reduced levels of growth regulators. Somatic embryos are being cultured for further development. .
AB  - U kulturi izolovanih zigotskih embriona i ovula munike (Pinus heldreichii) ispitivan je efekat hranljive podloge i regulatora rastenja na indukciju somatske embriogeneze. Somatska embriogeneza je indukovana u kulturi ovula koje su sadržavale embrione na ranom stupnju razvića. Najviša frekvencija indukcije od 6.7% postignuta je kada su ovule 5 dana gajene na Gresshoff i Doy (GD) hranljivoj podlozi u prisustvu 2,4-D 2 mg/l i BA 0.5 mg/l, a zatim prenete na GD podlogu u kojoj je koncentracija regulatora rastenja bila 5 puta niža. U cilju dugotrajnog održavanja u kulturi, embriogeno tkivo je izolovano i gajeno na GD podlozi sa 2,4-D 0.2 mg/l i BA 0.05 mg/l. Mikroskopskom analizom je utvrđeno da se tkivo sastoji od brojnih embriona na ranim stupnjevima razvića. Za klonalnu propagaciju ove značajne endemoreliktne četinarske vrste u kulturi in vitro, neophodna su dalja istraživanja sa ciljem povećanja frekvencije indukcije somatske embriogeneze, kao i frekvencije sazrevanja embriona. .
T2  - Archives of Biological Sciences
T1  - Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi
T1  - Induction of somatic embryogenesis in Pinus heldreichii culture
IS  - 3
VL  - 59
SP  - 199
EP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_159
ER  - 

@article{
author = "Stojičić, Dragana and Uzelac, Branka and Janošević, Dušica and Ćulafić, Ljubinka and Budimir, Snežana",
year = "2007, 2007",
abstract = "The potential for somatic embryogenesis in zygotic embryo and megagametophyte cultures of Pinus heldreichii was examined. Somatic embryogenesis was initiated from megagametophytes containing immature zygotic embryos at early stages of development. An induction frequency of up to 6.7% was obtained on Gresshoff and Doy medium in the presence of 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l benzyladenine (BA). Formation and further proliferation of embryogenic tissue were achieved upon transfer of explants to a medium with reduced levels of growth regulators. Somatic embryos are being cultured for further development. ., U kulturi izolovanih zigotskih embriona i ovula munike (Pinus heldreichii) ispitivan je efekat hranljive podloge i regulatora rastenja na indukciju somatske embriogeneze. Somatska embriogeneza je indukovana u kulturi ovula koje su sadržavale embrione na ranom stupnju razvića. Najviša frekvencija indukcije od 6.7% postignuta je kada su ovule 5 dana gajene na Gresshoff i Doy (GD) hranljivoj podlozi u prisustvu 2,4-D 2 mg/l i BA 0.5 mg/l, a zatim prenete na GD podlogu u kojoj je koncentracija regulatora rastenja bila 5 puta niža. U cilju dugotrajnog održavanja u kulturi, embriogeno tkivo je izolovano i gajeno na GD podlozi sa 2,4-D 0.2 mg/l i BA 0.05 mg/l. Mikroskopskom analizom je utvrđeno da se tkivo sastoji od brojnih embriona na ranim stupnjevima razvića. Za klonalnu propagaciju ove značajne endemoreliktne četinarske vrste u kulturi in vitro, neophodna su dalja istraživanja sa ciljem povećanja frekvencije indukcije somatske embriogeneze, kao i frekvencije sazrevanja embriona. .",
journal = "Archives of Biological Sciences",
title = "Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi, Induction of somatic embryogenesis in Pinus heldreichii culture",
number = "3",
volume = "59",
pages = "199-202",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_159"
}

Stojičić, D., Uzelac, B., Janošević, D., Ćulafić, L.,& Budimir, S.. (2007). Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi. in Archives of Biological Sciences, 59(3), 199-202.
https://hdl.handle.net/21.15107/rcub_ibiss_159

Stojičić D, Uzelac B, Janošević D, Ćulafić L, Budimir S. Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi. in Archives of Biological Sciences. 2007;59(3):199-202.
https://hdl.handle.net/21.15107/rcub_ibiss_159 .

Stojičić, Dragana, Uzelac, Branka, Janošević, Dušica, Ćulafić, Ljubinka, Budimir, Snežana, "Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi" in Archives of Biological Sciences, 59, no. 3 (2007):199-202,
https://hdl.handle.net/21.15107/rcub_ibiss_159 .