TY  - JOUR
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
AU  - Čokeša, Vlado
AU  - Uzelac, Branka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6521
AB  - Pinus peuce (Macedonian pine) is considered a valuable ornamental tree that is frequently planted in parks and gardens, especially in Western Europe. This endemic pine is one of the most valuable conifer species in its native range, which currently consists of only two disjunct populations restricted to small mountainous areas of the Balkans and is listed as a near-threatened species. The reproduction of Macedonian pine by seed is limited, so in vitro propagation methods have emerged as a promising tool for large-scale propagation. The objective of this study was to develop an improved system for the micropropagation of P. peuce from juvenile plant material using a short-term liquid cytokinin pulse. For that, explants derived from 4-week-old seedlings were pulse-treated with different concentrations of N6-benzyladenine (BA) for 1 or 2 h to stimulate the induction of axillary buds. The highest axillary shoot formation was achieved with 222 µM BA pulse treatment, with an average number of ~six shoots per explant. Elongated shoots (≥10 mm) were detached from the explants and pulse-treated with 0.27 or 1.08 mM α-naphthaleneacetic acid (NAA) or 0.25 or 0.98 mM indole-3-butyric acid (IBA) for 1 or 2 h. IBA was more effective than NAA and led to a maximum rooting percentage (up to 40%) and the highest number of acclimatized plants (15–20%). Rooted plants were successfully transferred to ex vitro conditions.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Optimization of In Vitro Regeneration of Pinus peuce (Gris.)
IS  - 1
VL  - 10
DO  - 10.3390/horticulturae10010097
SP  - 97
ER  - 

@article{
author = "Stojičić, Dragana and Budimir, Snežana and Čokeša, Vlado and Uzelac, Branka",
year = "2024",
abstract = "Pinus peuce (Macedonian pine) is considered a valuable ornamental tree that is frequently planted in parks and gardens, especially in Western Europe. This endemic pine is one of the most valuable conifer species in its native range, which currently consists of only two disjunct populations restricted to small mountainous areas of the Balkans and is listed as a near-threatened species. The reproduction of Macedonian pine by seed is limited, so in vitro propagation methods have emerged as a promising tool for large-scale propagation. The objective of this study was to develop an improved system for the micropropagation of P. peuce from juvenile plant material using a short-term liquid cytokinin pulse. For that, explants derived from 4-week-old seedlings were pulse-treated with different concentrations of N6-benzyladenine (BA) for 1 or 2 h to stimulate the induction of axillary buds. The highest axillary shoot formation was achieved with 222 µM BA pulse treatment, with an average number of ~six shoots per explant. Elongated shoots (≥10 mm) were detached from the explants and pulse-treated with 0.27 or 1.08 mM α-naphthaleneacetic acid (NAA) or 0.25 or 0.98 mM indole-3-butyric acid (IBA) for 1 or 2 h. IBA was more effective than NAA and led to a maximum rooting percentage (up to 40%) and the highest number of acclimatized plants (15–20%). Rooted plants were successfully transferred to ex vitro conditions.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Optimization of In Vitro Regeneration of Pinus peuce (Gris.)",
number = "1",
volume = "10",
doi = "10.3390/horticulturae10010097",
pages = "97"
}

Stojičić, D., Budimir, S., Čokeša, V.,& Uzelac, B.. (2024). Optimization of In Vitro Regeneration of Pinus peuce (Gris.). in Horticulturae
Basel : MDPI., 10(1), 97.
https://doi.org/10.3390/horticulturae10010097

Stojičić D, Budimir S, Čokeša V, Uzelac B. Optimization of In Vitro Regeneration of Pinus peuce (Gris.). in Horticulturae. 2024;10(1):97.
doi:10.3390/horticulturae10010097 .

Stojičić, Dragana, Budimir, Snežana, Čokeša, Vlado, Uzelac, Branka, "Optimization of In Vitro Regeneration of Pinus peuce (Gris.)" in Horticulturae, 10, no. 1 (2024):97,
https://doi.org/10.3390/horticulturae10010097 . .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Ćirić, Ana
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Anđelković, Boban
AU  - Todosijević, Marina
AU  - Todorović, Slađana
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Tešević, Vele
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Kanellis, Angelos K.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669022004757
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4983
AB  - Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites , such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus . Histochemical staining of leaves' trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus , grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) and two Gram-positive bacteria ( Bacillus cereus and Micrococcus flavus ). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent -3 β -acetoxy-13- epi -manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent -3 β -acetoxy-13- epi -manoyl oxide and its precursor ent -3 β -hydroxy-13- epi -manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”
VL  - 184
DO  - 10.1016/j.indcrop.2022.114992
SP  - 114992
ER  - 

@article{
author = "Skorić, Marijana and Ćirić, Ana and Budimir, Snežana and Janošević, Dušica and Anđelković, Boban and Todosijević, Marina and Todorović, Slađana and Soković, Marina and Glamočlija, Jasmina and Tešević, Vele and Gašić, Uroš and Mišić, Danijela and Kanellis, Angelos K.",
year = "2022",
abstract = "Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites , such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus . Histochemical staining of leaves' trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus , grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) and two Gram-positive bacteria ( Bacillus cereus and Micrococcus flavus ). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent -3 β -acetoxy-13- epi -manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent -3 β -acetoxy-13- epi -manoyl oxide and its precursor ent -3 β -hydroxy-13- epi -manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”",
volume = "184",
doi = "10.1016/j.indcrop.2022.114992",
pages = "114992"
}

Skorić, M., Ćirić, A., Budimir, S., Janošević, D., Anđelković, B., Todosijević, M., Todorović, S., Soković, M., Glamočlija, J., Tešević, V., Gašić, U., Mišić, D.,& Kanellis, A. K.. (2022). Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products
Elsevier B.V.., 184, 114992.
https://doi.org/10.1016/j.indcrop.2022.114992

Skorić M, Ćirić A, Budimir S, Janošević D, Anđelković B, Todosijević M, Todorović S, Soković M, Glamočlija J, Tešević V, Gašić U, Mišić D, Kanellis AK. Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products. 2022;184:114992.
doi:10.1016/j.indcrop.2022.114992 .

Skorić, Marijana, Ćirić, Ana, Budimir, Snežana, Janošević, Dušica, Anđelković, Boban, Todosijević, Marina, Todorović, Slađana, Soković, Marina, Glamočlija, Jasmina, Tešević, Vele, Gašić, Uroš, Mišić, Danijela, Kanellis, Angelos K., "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”" in Industrial Crops and Products, 184 (2022):114992,
https://doi.org/10.1016/j.indcrop.2022.114992 . .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Stojanović, Gordana
AU  - Zlatković, Bojan
AU  - Jovanović, Snežana
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/2/198
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8778064
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4768
AB  - Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography-mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.
IS  - 2
VL  - 11
DO  - 10.3390/plants11020198
SP  - 198
ER  - 

@article{
author = "Stojičić, Dragana and Tošić, Svetlana and Stojanović, Gordana and Zlatković, Bojan and Jovanović, Snežana and Budimir, Snežana and Uzelac, Branka",
year = "2022",
abstract = "Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography-mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.",
number = "2",
volume = "11",
doi = "10.3390/plants11020198",
pages = "198"
}

Stojičić, D., Tošić, S., Stojanović, G., Zlatković, B., Jovanović, S., Budimir, S.,& Uzelac, B.. (2022). Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.. in Plants (Basel, Switzerland)
Basel: MDPI., 11(2), 198.
https://doi.org/10.3390/plants11020198

Stojičić D, Tošić S, Stojanović G, Zlatković B, Jovanović S, Budimir S, Uzelac B. Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.. in Plants (Basel, Switzerland). 2022;11(2):198.
doi:10.3390/plants11020198 .

Stojičić, Dragana, Tošić, Svetlana, Stojanović, Gordana, Zlatković, Bojan, Jovanović, Snežana, Budimir, Snežana, Uzelac, Branka, "Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source." in Plants (Basel, Switzerland), 11, no. 2 (2022):198,
https://doi.org/10.3390/plants11020198 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Budimir, Snežana
AU  - Stojičić, Dragana
PY  - 2022
UR  - https://journal.pmf.ni.ac.rs/bionys/index.php/bionys/article/view/477
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5661
AB  - Many of the species belonging to the Lamiaceae family are considered aromatic
plants due to the presence of glandular trichomes, which have a distinct ability
to synthesize, secrete or store large amounts of specialized metabolites that
play a crucial role in mediating the plant–environment interactions. These
compounds often have marked bioactive properties, rendering a commercial
value to the plants that produce them. A number of biological effects have
been associated with the main monoterpenoids detected in investigated
Micromeria spp. and Clinopodium spp. essential oils. One alternative for the
production of these bioactive metabolites is in vitro plant tissue culture. The
present study was initiated to investigate the effects of in vitro culture on
the secretion of leaf glandular trichomes, the main structures involved in the
essential oil production. The glandular indumentum was studied by means of
light microscopy and scanning electron microscopy in an attempt to correlate
the phytochemical traits with the glandular trichome morphotypes of selected
Lamiaceae species.
PB  - Niš: University of Niš
T2  - Biologica Nyssana
T1  - Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach
IS  - 2
VL  - 13
DO  - 10.5281/zenodo.7476250
SP  - 191
EP  - 203
ER  - 

@article{
author = "Uzelac, Branka and Budimir, Snežana and Stojičić, Dragana",
year = "2022",
abstract = "Many of the species belonging to the Lamiaceae family are considered aromatic
plants due to the presence of glandular trichomes, which have a distinct ability
to synthesize, secrete or store large amounts of specialized metabolites that
play a crucial role in mediating the plant–environment interactions. These
compounds often have marked bioactive properties, rendering a commercial
value to the plants that produce them. A number of biological effects have
been associated with the main monoterpenoids detected in investigated
Micromeria spp. and Clinopodium spp. essential oils. One alternative for the
production of these bioactive metabolites is in vitro plant tissue culture. The
present study was initiated to investigate the effects of in vitro culture on
the secretion of leaf glandular trichomes, the main structures involved in the
essential oil production. The glandular indumentum was studied by means of
light microscopy and scanning electron microscopy in an attempt to correlate
the phytochemical traits with the glandular trichome morphotypes of selected
Lamiaceae species.",
publisher = "Niš: University of Niš",
journal = "Biologica Nyssana",
title = "Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach",
number = "2",
volume = "13",
doi = "10.5281/zenodo.7476250",
pages = "191-203"
}

Uzelac, B., Budimir, S.,& Stojičić, D.. (2022). Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach. in Biologica Nyssana
Niš: University of Niš., 13(2), 191-203.
https://doi.org/10.5281/zenodo.7476250

Uzelac B, Budimir S, Stojičić D. Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach. in Biologica Nyssana. 2022;13(2):191-203.
doi:10.5281/zenodo.7476250 .

Uzelac, Branka, Budimir, Snežana, Stojičić, Dragana, "Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach" in Biologica Nyssana, 13, no. 2 (2022):191-203,
https://doi.org/10.5281/zenodo.7476250 . .

TY  - CONF
AU  - Uzelac, Branka
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
AU  - Tošić, Svetlana
AU  - Zlatković, Bojan
AU  - Blagojević, Saša
AU  - Manić, Branislav
AU  - Janjanin, Mirjana
AU  - Slavkovska, Violeta
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5664
AB  - Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.
PB  - Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu
C3  - Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
T1  - Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach
DO  - 10.46793/SBT27.345U
SP  - 345
EP  - 350
ER  - 

@conference{
author = "Uzelac, Branka and Stojičić, Dragana and Budimir, Snežana and Tošić, Svetlana and Zlatković, Bojan and Blagojević, Saša and Manić, Branislav and Janjanin, Mirjana and Slavkovska, Violeta",
year = "2022",
abstract = "Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.",
publisher = "Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu",
journal = "Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia",
title = "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach",
doi = "10.46793/SBT27.345U",
pages = "345-350"
}

Uzelac, B., Stojičić, D., Budimir, S., Tošić, S., Zlatković, B., Blagojević, S., Manić, B., Janjanin, M.,& Slavkovska, V.. (2022). Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu., 345-350.
https://doi.org/10.46793/SBT27.345U

Uzelac B, Stojičić D, Budimir S, Tošić S, Zlatković B, Blagojević S, Manić B, Janjanin M, Slavkovska V. Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia. 2022;:345-350.
doi:10.46793/SBT27.345U .

Uzelac, Branka, Stojičić, Dragana, Budimir, Snežana, Tošić, Svetlana, Zlatković, Bojan, Blagojević, Saša, Manić, Branislav, Janjanin, Mirjana, Slavkovska, Violeta, "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach" in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia (2022):345-350,
https://doi.org/10.46793/SBT27.345U . .

TY  - CONF
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Zlatković, Bojan
AU  - Blagojević, Saša
AU  - Manić, Branislav
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5662
AB  - Clinopodium thymifolium (Scop.) Kuntze (syn. Micromeria thymifolia (Scop.) Fritsch) is a Balkan endemic species, traditionally used as a condiment and medicinal plant in the Mediterranean area. Clinopodium species were shown to produce considerable quantities (> 0.5%) of essential oils. The essential oil of wild-growing C. thymifolium possesses high antimicrobial activity. One alternative for the production of commercially important plant-derived metabolites, especially of rare plant genotypes, is in vitro plant tissue culture, given that the metabolic potential of in vitro shoot cultures can be manipulated by varying in vitro culture conditions. In view of the potential pharmacological and commercial value of C. thymifolium, the present study was initiated to propagate this aromatic plant species with the aim to increase the accumulation of biomass and the production of secondary metabolites. Shoots of wild-growing plants, dissected into one-node stem segments bearing two axillary buds, were used to establish in vitro cultures. Shoot multiplication was carried out on basal nutritional medium and on medium supplemented with various plant growth regulators at different concentrations. Nodal segments developed axillary shoots, and were successfully propagated on basal medium, whereas tested plant growth regulators differently affected their multiplication and biomass production.
PB  - Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
C3  - Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
T1  - Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5662
ER  - 

@conference{
editor = "Ranđelović, Vladimir, Stojanović-Radić, Zorica, Nikolić, Danijela, Jenačković Gocić, Dragana",
author = "Stojičić, Dragana and Tošić, Svetlana and Zlatković, Bojan and Blagojević, Saša and Manić, Branislav and Budimir, Snežana and Uzelac, Branka",
year = "2022",
abstract = "Clinopodium thymifolium (Scop.) Kuntze (syn. Micromeria thymifolia (Scop.) Fritsch) is a Balkan endemic species, traditionally used as a condiment and medicinal plant in the Mediterranean area. Clinopodium species were shown to produce considerable quantities (> 0.5%) of essential oils. The essential oil of wild-growing C. thymifolium possesses high antimicrobial activity. One alternative for the production of commercially important plant-derived metabolites, especially of rare plant genotypes, is in vitro plant tissue culture, given that the metabolic potential of in vitro shoot cultures can be manipulated by varying in vitro culture conditions. In view of the potential pharmacological and commercial value of C. thymifolium, the present study was initiated to propagate this aromatic plant species with the aim to increase the accumulation of biomass and the production of secondary metabolites. Shoots of wild-growing plants, dissected into one-node stem segments bearing two axillary buds, were used to establish in vitro cultures. Shoot multiplication was carried out on basal nutritional medium and on medium supplemented with various plant growth regulators at different concentrations. Nodal segments developed axillary shoots, and were successfully propagated on basal medium, whereas tested plant growth regulators differently affected their multiplication and biomass production.",
publisher = "Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš",
journal = "Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia",
title = "Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)",
pages = "113",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5662"
}

Ranđelović, V., Stojanović-Radić, Z., Nikolić, D., Jenačković Gocić, D., Stojičić, D., Tošić, S., Zlatković, B., Blagojević, S., Manić, B., Budimir, S.,& Uzelac, B.. (2022). Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae). in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 113.
https://hdl.handle.net/21.15107/rcub_ibiss_5662

Ranđelović V, Stojanović-Radić Z, Nikolić D, Jenačković Gocić D, Stojičić D, Tošić S, Zlatković B, Blagojević S, Manić B, Budimir S, Uzelac B. Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae). in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia. 2022;:113.
https://hdl.handle.net/21.15107/rcub_ibiss_5662 .

Ranđelović, Vladimir, Stojanović-Radić, Zorica, Nikolić, Danijela, Jenačković Gocić, Dragana, Stojičić, Dragana, Tošić, Svetlana, Zlatković, Bojan, Blagojević, Saša, Manić, Branislav, Budimir, Snežana, Uzelac, Branka, "Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)" in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia (2022):113,
https://hdl.handle.net/21.15107/rcub_ibiss_5662 .

TY  - CONF
AU  - Uzelac, Branka
AU  - Budimir, Snežana
AU  - Stojičić, Dragana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5663
AB  - Many of the species belonging to the Lamiaceae family are considered aromatic plants due to the presence of glandular trichomes, which have a distinct ability to synthesize, secrete or store large amounts of specialized metabolites that play a crucial role in mediating the plant–environment interactions. Secondary metabolites are biosynthesized as a defensive strategy of plants in response to natural perturbations, thus enabling them to adapt to the environmental stresses typical of each individual ecological niche. These compounds often have marked bioactive properties, rendering a commercial value to the plants that produce them. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these bioactive metabolites is in vitro plant tissue culture. The present study was initiated to investigate the effects of in vitro culture on the secretion of leaf glandular trichomes, the main structures involved in the essential oil production. The glandular indumentum was studied by means of light microscopy and scanning electron microscopy in an attempt to correlate the phytochemical traits with the glandular trichome morphotypes of selected Lamiaceae species.
PB  - Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
C3  - Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
T1  - Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach
SP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5663
ER  - 

@conference{
author = "Uzelac, Branka and Budimir, Snežana and Stojičić, Dragana",
year = "2022",
abstract = "Many of the species belonging to the Lamiaceae family are considered aromatic plants due to the presence of glandular trichomes, which have a distinct ability to synthesize, secrete or store large amounts of specialized metabolites that play a crucial role in mediating the plant–environment interactions. Secondary metabolites are biosynthesized as a defensive strategy of plants in response to natural perturbations, thus enabling them to adapt to the environmental stresses typical of each individual ecological niche. These compounds often have marked bioactive properties, rendering a commercial value to the plants that produce them. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these bioactive metabolites is in vitro plant tissue culture. The present study was initiated to investigate the effects of in vitro culture on the secretion of leaf glandular trichomes, the main structures involved in the essential oil production. The glandular indumentum was studied by means of light microscopy and scanning electron microscopy in an attempt to correlate the phytochemical traits with the glandular trichome morphotypes of selected Lamiaceae species.",
publisher = "Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš",
journal = "Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia",
title = "Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach",
pages = "120",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5663"
}

Uzelac, B., Budimir, S.,& Stojičić, D.. (2022). Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach. in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 120.
https://hdl.handle.net/21.15107/rcub_ibiss_5663

Uzelac B, Budimir S, Stojičić D. Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach. in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia. 2022;:120.
https://hdl.handle.net/21.15107/rcub_ibiss_5663 .

Uzelac, Branka, Budimir, Snežana, Stojičić, Dragana, "Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach" in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia (2022):120,
https://hdl.handle.net/21.15107/rcub_ibiss_5663 .

TY  - JOUR
AU  - Alimpić Aradski, Ana
AU  - Janošević, Dušica
AU  - Pećinar, Ilinka
AU  - Budimir, Snežana
AU  - Dajić Stevanović, Zora
AU  - Matevski, Vlado
AU  - Marin, Petar
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3631
AB  - In this study, we examined Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. collected in the Republic of North Macedonia, which are for the first time subjected to detailed micromorphological and structural analysis using light and scanning electron microscopy. The nutlets and mucilage were additionally subjected to spectroscopic analysis using Raman and Attenuated Total Reflectance Fourier Transform Infrared spectroscopy. The anatomical structure of stems and leaves is described and compared. The stems, leaves and calyces bear numerous one- and multi-cellular nonglandular trichomes, and various peltate, capitate and digitiform glandular trichomes. The nutlets differ in size and shape, as well as in myxocarpy. The nutlets predominantly contained α-linolenic and linoleic acid, whereas the mucilages are primarily formed of polysaccharides. The results obtained in this study confirmed the importance of micromorphological and anatomical analysis of Salvia L. spp. plant parts, particularly trichomes and nutlets, and hence contributing to the knowledge about the variety of micromorphological characteristics within the genus Salvia L.
PB  - Taylor and Francis Ltd.
T2  - Plant Biosystems
T1  - Micromorphological and anatomical characteristics of Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. (Lamiaceae)
IS  - 1
VL  - 155
DO  - 10.1080/11263504.2020.1727976
SP  - 92
EP  - 108
ER  - 

@article{
author = "Alimpić Aradski, Ana and Janošević, Dušica and Pećinar, Ilinka and Budimir, Snežana and Dajić Stevanović, Zora and Matevski, Vlado and Marin, Petar and Duletić-Laušević, Sonja",
year = "2021",
abstract = "In this study, we examined Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. collected in the Republic of North Macedonia, which are for the first time subjected to detailed micromorphological and structural analysis using light and scanning electron microscopy. The nutlets and mucilage were additionally subjected to spectroscopic analysis using Raman and Attenuated Total Reflectance Fourier Transform Infrared spectroscopy. The anatomical structure of stems and leaves is described and compared. The stems, leaves and calyces bear numerous one- and multi-cellular nonglandular trichomes, and various peltate, capitate and digitiform glandular trichomes. The nutlets differ in size and shape, as well as in myxocarpy. The nutlets predominantly contained α-linolenic and linoleic acid, whereas the mucilages are primarily formed of polysaccharides. The results obtained in this study confirmed the importance of micromorphological and anatomical analysis of Salvia L. spp. plant parts, particularly trichomes and nutlets, and hence contributing to the knowledge about the variety of micromorphological characteristics within the genus Salvia L.",
publisher = "Taylor and Francis Ltd.",
journal = "Plant Biosystems",
title = "Micromorphological and anatomical characteristics of Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. (Lamiaceae)",
number = "1",
volume = "155",
doi = "10.1080/11263504.2020.1727976",
pages = "92-108"
}

Alimpić Aradski, A., Janošević, D., Pećinar, I., Budimir, S., Dajić Stevanović, Z., Matevski, V., Marin, P.,& Duletić-Laušević, S.. (2021). Micromorphological and anatomical characteristics of Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. (Lamiaceae). in Plant Biosystems
Taylor and Francis Ltd.., 155(1), 92-108.
https://doi.org/10.1080/11263504.2020.1727976

Alimpić Aradski A, Janošević D, Pećinar I, Budimir S, Dajić Stevanović Z, Matevski V, Marin P, Duletić-Laušević S. Micromorphological and anatomical characteristics of Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. (Lamiaceae). in Plant Biosystems. 2021;155(1):92-108.
doi:10.1080/11263504.2020.1727976 .

Alimpić Aradski, Ana, Janošević, Dušica, Pećinar, Ilinka, Budimir, Snežana, Dajić Stevanović, Zora, Matevski, Vlado, Marin, Petar, Duletić-Laušević, Sonja, "Micromorphological and anatomical characteristics of Salvia amplexicaulis Lam., S. jurisicii Košanin and S. ringens Sibth. & Sm. (Lamiaceae)" in Plant Biosystems, 155, no. 1 (2021):92-108,
https://doi.org/10.1080/11263504.2020.1727976 . .

TY  - CHAP
AU  - Uzelac, Branka
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2020
UR  - https://link.springer.com/referenceworkentry/10.1007/978-3-030-11253-0_1-1
UR  - http://link.springer.com/10.1007/978-3-030-11253-0_1-1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3729
AB  - Glandular trichomes found on the surface of many higher plants contain specialized cells that produce and secrete copious amounts of particular secretory products. Leaf glandular trichomes of the non-model plant species Nicotiana tabacum represent a biologically active and stress-responsive tissue that contributes to plant defense response against biotic and abiotic stress and also influences leaf aroma and smoke flavor. Two morphologically different types of tobacco capitate trichomes, long- and short-stalked, with distinct functions, display ultrastructural features that are common to terpene-secreting glands, but only the secretory cells of the tall glandular trichomes are considered to be the site of biosynthesis of certain exudate compounds, including diterpenes and sucrose esters. Ultrastructural and histochemical characterization of tall glandular trichomes is described in an attempt to understand the contribution of these glands to the total secretion produced. Possible roles of distinct cellular compartments involved in the secretory process and secondary metabolite secretion under in vitro conditions are discussed.
PB  - Cham: Springer
T2  - Plant Cell and Tissue Differentiation and Secondary Metabolites
T1  - Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites
DO  - 10.1007/978-3-030-11253-0_1-1
ER  - 

@inbook{
author = "Uzelac, Branka and Stojičić, Dragana and Budimir, Snežana",
year = "2020",
abstract = "Glandular trichomes found on the surface of many higher plants contain specialized cells that produce and secrete copious amounts of particular secretory products. Leaf glandular trichomes of the non-model plant species Nicotiana tabacum represent a biologically active and stress-responsive tissue that contributes to plant defense response against biotic and abiotic stress and also influences leaf aroma and smoke flavor. Two morphologically different types of tobacco capitate trichomes, long- and short-stalked, with distinct functions, display ultrastructural features that are common to terpene-secreting glands, but only the secretory cells of the tall glandular trichomes are considered to be the site of biosynthesis of certain exudate compounds, including diterpenes and sucrose esters. Ultrastructural and histochemical characterization of tall glandular trichomes is described in an attempt to understand the contribution of these glands to the total secretion produced. Possible roles of distinct cellular compartments involved in the secretory process and secondary metabolite secretion under in vitro conditions are discussed.",
publisher = "Cham: Springer",
journal = "Plant Cell and Tissue Differentiation and Secondary Metabolites",
booktitle = "Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites",
doi = "10.1007/978-3-030-11253-0_1-1"
}

Uzelac, B., Stojičić, D.,& Budimir, S.. (2020). Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites. in Plant Cell and Tissue Differentiation and Secondary Metabolites
Cham: Springer..
https://doi.org/10.1007/978-3-030-11253-0_1-1

Uzelac B, Stojičić D, Budimir S. Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites. in Plant Cell and Tissue Differentiation and Secondary Metabolites. 2020;.
doi:10.1007/978-3-030-11253-0_1-1 .

Uzelac, Branka, Stojičić, Dragana, Budimir, Snežana, "Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites" in Plant Cell and Tissue Differentiation and Secondary Metabolites (2020),
https://doi.org/10.1007/978-3-030-11253-0_1-1 . .

TY  - JOUR
AU  - Cvetić Antić, Tijana
AU  - Janošević, Dušica
AU  - Maksimović, Vuk M.
AU  - Živić, Miroslav
AU  - Budimir, Snežana
AU  - Glamočlija, Jasmina
AU  - Mitrović, Aleksandra Lj
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3730
AB  - We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
PB  - NLM (Medline)
T2  - Journal of Plant Physiology
T1  - Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
VL  - 244
DO  - 10.1016/j.jplph.2019.153086
SP  - 153086
ER  - 

@article{
author = "Cvetić Antić, Tijana and Janošević, Dušica and Maksimović, Vuk M. and Živić, Miroslav and Budimir, Snežana and Glamočlija, Jasmina and Mitrović, Aleksandra Lj",
year = "2020",
abstract = "We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.",
publisher = "NLM (Medline)",
journal = "Journal of Plant Physiology",
title = "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro",
volume = "244",
doi = "10.1016/j.jplph.2019.153086",
pages = "153086"
}

Cvetić Antić, T., Janošević, D., Maksimović, V. M., Živić, M., Budimir, S., Glamočlija, J.,& Mitrović, A. L.. (2020). Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology
NLM (Medline)., 244, 153086.
https://doi.org/10.1016/j.jplph.2019.153086

Cvetić Antić T, Janošević D, Maksimović VM, Živić M, Budimir S, Glamočlija J, Mitrović AL. Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology. 2020;244:153086.
doi:10.1016/j.jplph.2019.153086 .

Cvetić Antić, Tijana, Janošević, Dušica, Maksimović, Vuk M., Živić, Miroslav, Budimir, Snežana, Glamočlija, Jasmina, Mitrović, Aleksandra Lj, "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro" in Journal of Plant Physiology, 244 (2020):153086,
https://doi.org/10.1016/j.jplph.2019.153086 . .

TY  - JOUR
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Slavkovska, Violeta
AU  - Mihailov-Krstev, Tatjana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - http://link.springer.com/10.1007/s00425-018-03071-5
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3256
AB  - MAIN CONCLUSION In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.
PB  - Springer Berlin Heidelberg
T2  - Planta
T1  - Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).
DO  - 10.1007/s00425-018-03071-5
ER  - 

@article{
author = "Tošić, Svetlana and Stojičić, Dragana and Slavkovska, Violeta and Mihailov-Krstev, Tatjana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
abstract = "MAIN CONCLUSION In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.",
publisher = "Springer Berlin Heidelberg",
journal = "Planta",
title = "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).",
doi = "10.1007/s00425-018-03071-5"
}

Tošić, S., Stojičić, D., Slavkovska, V., Mihailov-Krstev, T., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).. in Planta
Springer Berlin Heidelberg..
https://doi.org/10.1007/s00425-018-03071-5

Tošić S, Stojičić D, Slavkovska V, Mihailov-Krstev T, Zlatković B, Budimir S, Uzelac B. Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).. in Planta. 2019;.
doi:10.1007/s00425-018-03071-5 .

Tošić, Svetlana, Stojičić, Dragana, Slavkovska, Violeta, Mihailov-Krstev, Tatjana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)." in Planta (2019),
https://doi.org/10.1007/s00425-018-03071-5 . .

TY  - CHAP
AU  - Stojičić, Dragana
AU  - Uzelac, Branka
AU  - Budimir, Snežana
PY  - 2018
UR  - http://link.springer.com/10.1007/978-3-319-89483-6_4
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3270
AB  - Bosnian pine (Pinus heldreichii Christ.) is a Tertiary relict subendemic to the mountains of Balkan Peninsula and a small area in southern Italy.
PB  - Springer
T2  - Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants
T1  - Bosnian Pine Pinus heldreichii Christ.
DO  - 10.1007/978-3-319-89483-6_4
SP  - 49
EP  - 62
ER  - 

@inbook{
author = "Stojičić, Dragana and Uzelac, Branka and Budimir, Snežana",
year = "2018",
abstract = "Bosnian pine (Pinus heldreichii Christ.) is a Tertiary relict subendemic to the mountains of Balkan Peninsula and a small area in southern Italy.",
publisher = "Springer",
journal = "Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants",
booktitle = "Bosnian Pine Pinus heldreichii Christ.",
doi = "10.1007/978-3-319-89483-6_4",
pages = "49-62"
}

Stojičić, D., Uzelac, B.,& Budimir, S.. (2018). Bosnian Pine Pinus heldreichii Christ.. in Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants
Springer., 49-62.
https://doi.org/10.1007/978-3-319-89483-6_4

Stojičić D, Uzelac B, Budimir S. Bosnian Pine Pinus heldreichii Christ.. in Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. 2018;:49-62.
doi:10.1007/978-3-319-89483-6_4 .

Stojičić, Dragana, Uzelac, Branka, Budimir, Snežana, "Bosnian Pine Pinus heldreichii Christ." in Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants (2018):49-62,
https://doi.org/10.1007/978-3-319-89483-6_4 . .

TY  - CHAP
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Budimir, Snežana
PY  - 2018
UR  - http://link.springer.com/10.1007/978-1-4939-7672-0_21
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3000
AB  - Leaf senescence constitutes a highly regulated final phase of leaf development, leading to cell death that is recognized as a type of programmed cell death (PCD). Degradation of nuclear DNA into oligonucleosomal fragments (DNA ladder) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay are methods commonly used to detect PCD-specific DNA cleavage. TUNEL reaction in situ labels free 3′-OH DNA strand breaks (nicks), thus allowing histological localization of nuclear DNA degradation during PCD. Here we describe in situ labeling of PCD-specific nuclear DNA fragmentation on conventional histological sections of senescing tobacco leaves. Incorporation of fluorescein-labeled dUTPs is detected by fluorescence microscopy, which enables in situ visualization of PCD at the single-cell level in the leaf mesophyll tissues undergoing senescence.
PB  - Humana Press, New York, NY
T2  - Plant Senescence: Methods and Protocols
T2  - Plant Senescence: Methods and Protocols
T1  - In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay
DO  - 10.1007/978-1-4939-7672-0_21
SP  - 267
EP  - 282
ER  - 

@inbook{
author = "Uzelac, Branka and Janošević, Dušica and Budimir, Snežana",
year = "2018",
abstract = "Leaf senescence constitutes a highly regulated final phase of leaf development, leading to cell death that is recognized as a type of programmed cell death (PCD). Degradation of nuclear DNA into oligonucleosomal fragments (DNA ladder) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay are methods commonly used to detect PCD-specific DNA cleavage. TUNEL reaction in situ labels free 3′-OH DNA strand breaks (nicks), thus allowing histological localization of nuclear DNA degradation during PCD. Here we describe in situ labeling of PCD-specific nuclear DNA fragmentation on conventional histological sections of senescing tobacco leaves. Incorporation of fluorescein-labeled dUTPs is detected by fluorescence microscopy, which enables in situ visualization of PCD at the single-cell level in the leaf mesophyll tissues undergoing senescence.",
publisher = "Humana Press, New York, NY",
journal = "Plant Senescence: Methods and Protocols, Plant Senescence: Methods and Protocols",
booktitle = "In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay",
doi = "10.1007/978-1-4939-7672-0_21",
pages = "267-282"
}

Uzelac, B., Janošević, D.,& Budimir, S.. (2018). In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay. in Plant Senescence: Methods and Protocols
Humana Press, New York, NY., 267-282.
https://doi.org/10.1007/978-1-4939-7672-0_21

Uzelac B, Janošević D, Budimir S. In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay. in Plant Senescence: Methods and Protocols. 2018;:267-282.
doi:10.1007/978-1-4939-7672-0_21 .

Uzelac, Branka, Janošević, Dušica, Budimir, Snežana, "In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay" in Plant Senescence: Methods and Protocols (2018):267-282,
https://doi.org/10.1007/978-1-4939-7672-0_21 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2017
UR  - http://doi.wiley.com/10.1002/jemt.22864
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2681
AB  - The anatomy and ultrastructure of the short glandular trichomes occurring on young expanding leaves of Nicotiana tabacum were investigated using light and transmission electron microscopy. The objective of the present research was to characterize the cellular changes that occur during morphogenesis of short glandular trichomes, from initiation to senescence. Ultrastructural analysis of their secretory cells revealed characteristics common to gland cells: numerous mitochondria in highly organized cytoplasm, large nuclei, and an elaborate network of endoplasmic reticulum. Initial changes in nuclear and plastidial organization were observed at a more advanced secretory stage, marking the onset of senescence. During trichome senescence, gradual reduction of the cytoplasm density occurred along with structural changes of the plastids and the tonoplast. As a result of inward blebbing of the cytoplasm into the vacuole, membrane bound vesicular structures appeared in the vacuolar space. At the late secretory stage, marked by an increase in vacuolation and extraplasmic space, degenerative changes included further fragmentation of the cytoplasm and deterioration of the tonoplast. Multimembrane myelin bodies observed in the vacuolar space were indicative of membrane digestion although plasma membrane did not appear massively degraded.
T2  - Microscopy Research and Technique
T1  - Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes
DO  - 10.1002/jemt.22864
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2017",
abstract = "The anatomy and ultrastructure of the short glandular trichomes occurring on young expanding leaves of Nicotiana tabacum were investigated using light and transmission electron microscopy. The objective of the present research was to characterize the cellular changes that occur during morphogenesis of short glandular trichomes, from initiation to senescence. Ultrastructural analysis of their secretory cells revealed characteristics common to gland cells: numerous mitochondria in highly organized cytoplasm, large nuclei, and an elaborate network of endoplasmic reticulum. Initial changes in nuclear and plastidial organization were observed at a more advanced secretory stage, marking the onset of senescence. During trichome senescence, gradual reduction of the cytoplasm density occurred along with structural changes of the plastids and the tonoplast. As a result of inward blebbing of the cytoplasm into the vacuole, membrane bound vesicular structures appeared in the vacuolar space. At the late secretory stage, marked by an increase in vacuolation and extraplasmic space, degenerative changes included further fragmentation of the cytoplasm and deterioration of the tonoplast. Multimembrane myelin bodies observed in the vacuolar space were indicative of membrane digestion although plasma membrane did not appear massively degraded.",
journal = "Microscopy Research and Technique",
title = "Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes",
doi = "10.1002/jemt.22864"
}

Uzelac, B., Janošević, D., Stojičić, D.,& Budimir, S.. (2017). Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes. in Microscopy Research and Technique.
https://doi.org/10.1002/jemt.22864

Uzelac B, Janošević D, Stojičić D, Budimir S. Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes. in Microscopy Research and Technique. 2017;.
doi:10.1002/jemt.22864 .

Uzelac, Branka, Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes" in Microscopy Research and Technique (2017),
https://doi.org/10.1002/jemt.22864 . .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6200
AB  - The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.
PB  - Berlin Heidelberg : Springer-Verlag
T2  - Planta
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)
IS  - 2
VL  - 244
DO  - 10.1007/s00425-016-2513-7
SP  - 393
EP  - 404
ER  - 

@article{
author = "Stojičić, Dragana and Tošić, Svetlana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Janošević, Dušica and Uzelac, Branka",
year = "2016",
abstract = "The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.",
publisher = "Berlin Heidelberg : Springer-Verlag",
journal = "Planta",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)",
number = "2",
volume = "244",
doi = "10.1007/s00425-016-2513-7",
pages = "393-404"
}

Stojičić, D., Tošić, S., Slavkovska, V., Zlatković, B., Budimir, S., Janošević, D.,& Uzelac, B.. (2016). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta
Berlin Heidelberg : Springer-Verlag., 244(2), 393-404.
https://doi.org/10.1007/s00425-016-2513-7

Stojičić D, Tošić S, Slavkovska V, Zlatković B, Budimir S, Janošević D, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta. 2016;244(2):393-404.
doi:10.1007/s00425-016-2513-7 .

Stojičić, Dragana, Tošić, Svetlana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Janošević, Dušica, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)" in Planta, 244, no. 2 (2016):393-404,
https://doi.org/10.1007/s00425-016-2513-7 . .

TY  - JOUR
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Banjac, Nevena
AU  - Milojević, Jelena
AU  - Janošević, Dušica
AU  - Budimir, Snežana
AU  - Zdravković-Korać, Snežana
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4358
AB  - Unlike garlic and onion, the regeneration of chive (Allium schoenoprasum L.), cultivated both for culinary and ornamental purposes, has not been intensively studied. The effects of the eight cytokinins and the plant basal section thickness on regeneration efficiency and subsequent plant growth were studied. Representatives of all cytokinin structural groups: isoprenoide side chain (trans-zeatin) and aromatic side chain (benziladenine, kinetin, meta-topolin) adenine derivatives, and phenylurea derivatives (thidiazuron and N-(2-chloro-4-pyridyl)-N′-phenylurea) at 0, 1, 5 or 10 μM were used. Histological analysis revealed adventitious buds formation from the leaf sheaths’ bases and the basal plate. The highest regeneration frequency (100 %) and the mean bud number per explant (20.0) were achieved with 10 μM thidiazuron (TDZ), and 5 mm-thick basal sections were the most responsive explants. Inferior shoot and root growth characteristics of plants regenerated by this treatment was avoided by exclusion or replacement of 10 μM TDZ with 5 μM kinetin (Kin) after a 4-week bud induction period, without consequences on the regeneration efficiency. In addition, a positive correlation between peroxidase, catalase and superoxide dismutase activity and the regeneration capacity was observed. All antioxidative enzymes activity changed much faster with 10 μM TDZ than with 1 μM Kin, which provoked the weakest regeneration response. Moreover, a unique peroxidase isoform was observed only in TDZ-treated explants after 3rd day of treatment. This work is useful for genetic engineering and virus-free plant production advancement, and for the knowledge expansion regarding the role of antioxidative enzymes in plant organogenesis.
PB  - Dordrecht: Springer
T2  - Plant Cell Tissue and Organ Culture
T1  - Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)
IS  - 1
VL  - 124
DO  - 10.1007/s11240-015-0869-1
SP  - 1
EP  - 14
ER  - 

@article{
author = "Tubić, Ljiljana and Savić, Jelena and Banjac, Nevena and Milojević, Jelena and Janošević, Dušica and Budimir, Snežana and Zdravković-Korać, Snežana",
year = "2016",
abstract = "Unlike garlic and onion, the regeneration of chive (Allium schoenoprasum L.), cultivated both for culinary and ornamental purposes, has not been intensively studied. The effects of the eight cytokinins and the plant basal section thickness on regeneration efficiency and subsequent plant growth were studied. Representatives of all cytokinin structural groups: isoprenoide side chain (trans-zeatin) and aromatic side chain (benziladenine, kinetin, meta-topolin) adenine derivatives, and phenylurea derivatives (thidiazuron and N-(2-chloro-4-pyridyl)-N′-phenylurea) at 0, 1, 5 or 10 μM were used. Histological analysis revealed adventitious buds formation from the leaf sheaths’ bases and the basal plate. The highest regeneration frequency (100 %) and the mean bud number per explant (20.0) were achieved with 10 μM thidiazuron (TDZ), and 5 mm-thick basal sections were the most responsive explants. Inferior shoot and root growth characteristics of plants regenerated by this treatment was avoided by exclusion or replacement of 10 μM TDZ with 5 μM kinetin (Kin) after a 4-week bud induction period, without consequences on the regeneration efficiency. In addition, a positive correlation between peroxidase, catalase and superoxide dismutase activity and the regeneration capacity was observed. All antioxidative enzymes activity changed much faster with 10 μM TDZ than with 1 μM Kin, which provoked the weakest regeneration response. Moreover, a unique peroxidase isoform was observed only in TDZ-treated explants after 3rd day of treatment. This work is useful for genetic engineering and virus-free plant production advancement, and for the knowledge expansion regarding the role of antioxidative enzymes in plant organogenesis.",
publisher = "Dordrecht: Springer",
journal = "Plant Cell Tissue and Organ Culture",
title = "Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)",
number = "1",
volume = "124",
doi = "10.1007/s11240-015-0869-1",
pages = "1-14"
}

Tubić, L., Savić, J., Banjac, N., Milojević, J., Janošević, D., Budimir, S.,& Zdravković-Korać, S.. (2016). Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.). in Plant Cell Tissue and Organ Culture
Dordrecht: Springer., 124(1), 1-14.
https://doi.org/10.1007/s11240-015-0869-1

Tubić L, Savić J, Banjac N, Milojević J, Janošević D, Budimir S, Zdravković-Korać S. Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.). in Plant Cell Tissue and Organ Culture. 2016;124(1):1-14.
doi:10.1007/s11240-015-0869-1 .

Tubić, Ljiljana, Savić, Jelena, Banjac, Nevena, Milojević, Jelena, Janošević, Dušica, Budimir, Snežana, Zdravković-Korać, Snežana, "Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)" in Plant Cell Tissue and Organ Culture, 124, no. 1 (2016):1-14,
https://doi.org/10.1007/s11240-015-0869-1 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Simonović, Ana
AU  - Motyka, Václav
AU  - Dobrev, Petre I
AU  - Budimir, Snežana
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2989
UR  - https://link.springer.com/article/10.1007%2Fs00709-015-0802-9#aboutcontent
AB  - Leaf senescence is a highly regulated final phase of leaf development preceding massive cell death. It results in the coordinated degradation of macromolecules and the subsequent nutrient relocation to other plant parts. Very little is still known about early stages of leaf senescence during normal leaf ontogeny that is not triggered by stress factors. This paper comprises an integrated study of natural leaf senescence in tobacco plants grown in vitro, using molecular, structural, and physiological information. We determined the time sequence of ultrastructural changes in mesophyll cells during leaf senescence, showing that the degradation of chloroplast ultrastructure fully correlated with changes in chlorophyll content. The earliest degenerative changes in chloroplast ultrastructure coinciding with early chromatin condensation were observed already in mature green leaves. A continuum of degradative changes in chloroplast ultrastructure, chromatin condensation and aggregation, along with progressive decrease in cytoplasm organization and electron density were observed in the course of mesophyll cells ageing. Although the total amounts of endogenous cytokinins gradually increased during leaf ontogenesis, the proportion of bioactive cytokinin forms, as well as their phosphate precursors, in total cytokinin content rapidly declined with ageing. Endogenous indole-3-acetic acid (IAA) levels were strongly reduced in senescent leaves, and a decreasing tendency was also observed for abscisic acid (ABA) levels. Senescence-associated tobacco cysteine proteases (CP, E.C. 3.4.22) CP1 and CP23 genes were induced in the initial phase of senescence. Genes encoding glutamate dehydrogenase (GDH, E.C. 1.4.1.2) and one isoform of cytosolic glutamine synthetase (GS1, E.C. 6.3.1.2) were induced in the late stage of senescence, while chloroplastic GS (GS2) gene showed a continuous decrease with leaf ageing.
PB  - Springer-Verlag Wien
T2  - Protoplasma
T1  - Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.
IS  - 2
VL  - 253
DO  - 10.1007/s00709-015-0802-9
SP  - 259
EP  - 75
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Simonović, Ana and Motyka, Václav and Dobrev, Petre I and Budimir, Snežana",
year = "2016",
abstract = "Leaf senescence is a highly regulated final phase of leaf development preceding massive cell death. It results in the coordinated degradation of macromolecules and the subsequent nutrient relocation to other plant parts. Very little is still known about early stages of leaf senescence during normal leaf ontogeny that is not triggered by stress factors. This paper comprises an integrated study of natural leaf senescence in tobacco plants grown in vitro, using molecular, structural, and physiological information. We determined the time sequence of ultrastructural changes in mesophyll cells during leaf senescence, showing that the degradation of chloroplast ultrastructure fully correlated with changes in chlorophyll content. The earliest degenerative changes in chloroplast ultrastructure coinciding with early chromatin condensation were observed already in mature green leaves. A continuum of degradative changes in chloroplast ultrastructure, chromatin condensation and aggregation, along with progressive decrease in cytoplasm organization and electron density were observed in the course of mesophyll cells ageing. Although the total amounts of endogenous cytokinins gradually increased during leaf ontogenesis, the proportion of bioactive cytokinin forms, as well as their phosphate precursors, in total cytokinin content rapidly declined with ageing. Endogenous indole-3-acetic acid (IAA) levels were strongly reduced in senescent leaves, and a decreasing tendency was also observed for abscisic acid (ABA) levels. Senescence-associated tobacco cysteine proteases (CP, E.C. 3.4.22) CP1 and CP23 genes were induced in the initial phase of senescence. Genes encoding glutamate dehydrogenase (GDH, E.C. 1.4.1.2) and one isoform of cytosolic glutamine synthetase (GS1, E.C. 6.3.1.2) were induced in the late stage of senescence, while chloroplastic GS (GS2) gene showed a continuous decrease with leaf ageing.",
publisher = "Springer-Verlag Wien",
journal = "Protoplasma",
title = "Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.",
number = "2",
volume = "253",
doi = "10.1007/s00709-015-0802-9",
pages = "259-75"
}

Uzelac, B., Janošević, D., Simonović, A., Motyka, V., Dobrev, P. I.,& Budimir, S.. (2016). Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.. in Protoplasma
Springer-Verlag Wien., 253(2), 259-75.
https://doi.org/10.1007/s00709-015-0802-9

Uzelac B, Janošević D, Simonović A, Motyka V, Dobrev PI, Budimir S. Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.. in Protoplasma. 2016;253(2):259-75.
doi:10.1007/s00709-015-0802-9 .

Uzelac, Branka, Janošević, Dušica, Simonović, Ana, Motyka, Václav, Dobrev, Petre I, Budimir, Snežana, "Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro." in Protoplasma, 253, no. 2 (2016):259-75,
https://doi.org/10.1007/s00709-015-0802-9 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2015
UR  - https://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2015_39_2_632_full.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4063
AB  - The tall glandular trichomes occurring on young expanding leaves of Nicotiana tabacum grown in
vitro were investigated using light and electron microscopy. Previous studies reported that the tall
glandular trichomes of tobacco produce large quantities of various compounds including diterpenes.
To explore the cellular structures required and putatively involved in diterpene biosynthesis and
secretion, the present study focused on the development of the tall glandular trichomes, and on
histochemical and ultrastructural analysis of their secretory cells under in vitro conditions. Electron
microscopy confirmed earlier light microscopy observations of the glandular nature of the tall leaf
trichomes. Their head cells, which secrete resinous material, exhibited characteristics common
to gland cells: a dense cytoplasm, numerous mitochondria and little vacuolation. They contained
structurally well-developed chloroplasts and an elaborate network of endoplasmic reticulum, as
well as electron dense inclusions in chloroplasts and in small vacuoles. The dominance of smooth
endoplasmic reticulum suggests its involvement in diterpene biosynthesis. Histochemical tests were
positive for both lipophilic and hydrophilic secretions, which included terpenoids (essential oils
and resins) and acidic/neutral lipids, as well as phenolic compounds (tannins), alkaloids, mucilage/
pectin and polysaccharides.
PB  - Belgrade: Institute of Botany and Botanical Garden "Jevremovac"
T2  - Botanica Serbica
T1  - In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes
IS  - 2
VL  - 39
SP  - 103
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4063
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2015",
abstract = "The tall glandular trichomes occurring on young expanding leaves of Nicotiana tabacum grown in
vitro were investigated using light and electron microscopy. Previous studies reported that the tall
glandular trichomes of tobacco produce large quantities of various compounds including diterpenes.
To explore the cellular structures required and putatively involved in diterpene biosynthesis and
secretion, the present study focused on the development of the tall glandular trichomes, and on
histochemical and ultrastructural analysis of their secretory cells under in vitro conditions. Electron
microscopy confirmed earlier light microscopy observations of the glandular nature of the tall leaf
trichomes. Their head cells, which secrete resinous material, exhibited characteristics common
to gland cells: a dense cytoplasm, numerous mitochondria and little vacuolation. They contained
structurally well-developed chloroplasts and an elaborate network of endoplasmic reticulum, as
well as electron dense inclusions in chloroplasts and in small vacuoles. The dominance of smooth
endoplasmic reticulum suggests its involvement in diterpene biosynthesis. Histochemical tests were
positive for both lipophilic and hydrophilic secretions, which included terpenoids (essential oils
and resins) and acidic/neutral lipids, as well as phenolic compounds (tannins), alkaloids, mucilage/
pectin and polysaccharides.",
publisher = "Belgrade: Institute of Botany and Botanical Garden "Jevremovac"",
journal = "Botanica Serbica",
title = "In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes",
number = "2",
volume = "39",
pages = "103-110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4063"
}

Uzelac, B., Janošević, D., Stojičić, D.,& Budimir, S.. (2015). In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden "Jevremovac"., 39(2), 103-110.
https://hdl.handle.net/21.15107/rcub_ibiss_4063

Uzelac B, Janošević D, Stojičić D, Budimir S. In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes. in Botanica Serbica. 2015;39(2):103-110.
https://hdl.handle.net/21.15107/rcub_ibiss_4063 .

Uzelac, Branka, Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes" in Botanica Serbica, 39, no. 2 (2015):103-110,
https://hdl.handle.net/21.15107/rcub_ibiss_4063 .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Banjac, Nevena
AU  - Budimir, Snežana
AU  - Janošević, D.
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2095
UR  - https://s100.copyright.com/AppDispatchServlet?publisherName=ELS&contentID=S0254629914001938&orderBeanReset=true
AB  - Chenopodium murals L. wild-type roots (WR) and hairy roots (HR) exudates
   have been previously shown to exert allelopathic activity against test
   plant species lettuce, wheat and Arabidopsis. To further investigate
   their allelopathic nature, a comparative morpho-histological
   characterization and total phenolic content (TPC) evaluation were
   performed. Furthermore, the phytotoxic activity of WR and HR clone R5
   against three weed species and their antioxidant responses were also
   assessed. Except for the higher degree of branching and root hair
   incidence in HR clones, both WR and HR showed similar anatomical
   features, typical for higher plants. No significant difference in total
   phenolic content between WR and HRs, nor their exudates was found. Root
   exudates of WR and R5, applied as growth medium (GM) wherein these C.
   murale roots were cultured, displayed selective phytotoxic activity
   depending on the target weed species, ranging from suppression of
   germination to inhibition of seedling growth, that were linked with
   alterations in antioxidant enzyme activities. R5 greatly inhibited
   germination and seedling growth of Capsella bursa-pastoris, while WR
   inhibited those of Vicia angustifolia and Amaranthus retroflexus. Such
   phytotoxic properties (of WR and R5) qualify them as a promising natural
   resource in the management of weeds. Differences in allelopathic
   activity between HR and WR should be searched for in their
   allelochemical profile and the content of each allelopathic substance.
   The lack of anatomical abnormalities in hairy roots renders them an
   efficient tool for functional-genomic studies of C. murale root genes.
   (C) 2014 SAAB. Published by Elsevier B.V. All rights reserved.
T2  - South African Journal of Botany
T1  - Morpho-histological and bioherbicidal evaluation of wild-type and
 transformed hairy roots of goosefoot
VL  - 96
DO  - 10.1016/j.sajb.2014.11.002
SP  - 53
EP  - 61
ER  - 

@article{
author = "Dmitrović, Slavica and Banjac, Nevena and Budimir, Snežana and Janošević, D. and Živković, Suzana and Skorić, Marijana and Ninković, Slavica",
year = "2015",
abstract = "Chenopodium murals L. wild-type roots (WR) and hairy roots (HR) exudates
   have been previously shown to exert allelopathic activity against test
   plant species lettuce, wheat and Arabidopsis. To further investigate
   their allelopathic nature, a comparative morpho-histological
   characterization and total phenolic content (TPC) evaluation were
   performed. Furthermore, the phytotoxic activity of WR and HR clone R5
   against three weed species and their antioxidant responses were also
   assessed. Except for the higher degree of branching and root hair
   incidence in HR clones, both WR and HR showed similar anatomical
   features, typical for higher plants. No significant difference in total
   phenolic content between WR and HRs, nor their exudates was found. Root
   exudates of WR and R5, applied as growth medium (GM) wherein these C.
   murale roots were cultured, displayed selective phytotoxic activity
   depending on the target weed species, ranging from suppression of
   germination to inhibition of seedling growth, that were linked with
   alterations in antioxidant enzyme activities. R5 greatly inhibited
   germination and seedling growth of Capsella bursa-pastoris, while WR
   inhibited those of Vicia angustifolia and Amaranthus retroflexus. Such
   phytotoxic properties (of WR and R5) qualify them as a promising natural
   resource in the management of weeds. Differences in allelopathic
   activity between HR and WR should be searched for in their
   allelochemical profile and the content of each allelopathic substance.
   The lack of anatomical abnormalities in hairy roots renders them an
   efficient tool for functional-genomic studies of C. murale root genes.
   (C) 2014 SAAB. Published by Elsevier B.V. All rights reserved.",
journal = "South African Journal of Botany",
title = "Morpho-histological and bioherbicidal evaluation of wild-type and
 transformed hairy roots of goosefoot",
volume = "96",
doi = "10.1016/j.sajb.2014.11.002",
pages = "53-61"
}

Dmitrović, S., Banjac, N., Budimir, S., Janošević, D., Živković, S., Skorić, M.,& Ninković, S.. (2015). Morpho-histological and bioherbicidal evaluation of wild-type and
 transformed hairy roots of goosefoot. in South African Journal of Botany, 96, 53-61.
https://doi.org/10.1016/j.sajb.2014.11.002

Dmitrović S, Banjac N, Budimir S, Janošević D, Živković S, Skorić M, Ninković S. Morpho-histological and bioherbicidal evaluation of wild-type and
 transformed hairy roots of goosefoot. in South African Journal of Botany. 2015;96:53-61.
doi:10.1016/j.sajb.2014.11.002 .

Dmitrović, Slavica, Banjac, Nevena, Budimir, Snežana, Janošević, D., Živković, Suzana, Skorić, Marijana, Ninković, Slavica, "Morpho-histological and bioherbicidal evaluation of wild-type and
 transformed hairy roots of goosefoot" in South African Journal of Botany, 96 (2015):53-61,
https://doi.org/10.1016/j.sajb.2014.11.002 . .

TY  - JOUR
AU  - Al Sheef Najat, Beleed
AU  - Duletić-Laušević, Sonja N.
AU  - Janošević, Dušica
AU  - Budimir, Snežana
AU  - Marin, Marija
AU  - Alimpić, Ana
AU  - Giweli, A.A.M.
AU  - Marin, P.D.
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/327
AB  - Micromorphological and ultrastructural analyses of the leaf trichomes of Salvia fruticosa Mill. were performed by light and electron microscopy. The leaves bear numerous non-glandular unbranched trichomes, and peltate, capitate and digitiform glandular trichomes. Very elongated flagelliform non-glandular trichomes densely covered the leaf surfaces, with especially abundance on the leaf margins. Peltate trichomes consist of a basal epidermal cell, a very short stalk cell and a large round head of eight secretory cells arranged in a circle. Capitate trichomes can be divided into two main types, short-stalked and long-stalked, and further into five subtypes according to the number of stalk cells, morphology and number of glandular head cells. Digitiform trichomes consist of one basal cell, one or two stalk cells and one apical secretory cell, which are of similar diameter and approximately equal length.
T2  - Archives of Biological Sciences
T1  - Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill.
IS  - 1
VL  - 65
SP  - 239
EP  - 246
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_327
ER  - 

@article{
author = "Al Sheef Najat, Beleed and Duletić-Laušević, Sonja N. and Janošević, Dušica and Budimir, Snežana and Marin, Marija and Alimpić, Ana and Giweli, A.A.M. and Marin, P.D.",
year = "2013, 2013",
abstract = "Micromorphological and ultrastructural analyses of the leaf trichomes of Salvia fruticosa Mill. were performed by light and electron microscopy. The leaves bear numerous non-glandular unbranched trichomes, and peltate, capitate and digitiform glandular trichomes. Very elongated flagelliform non-glandular trichomes densely covered the leaf surfaces, with especially abundance on the leaf margins. Peltate trichomes consist of a basal epidermal cell, a very short stalk cell and a large round head of eight secretory cells arranged in a circle. Capitate trichomes can be divided into two main types, short-stalked and long-stalked, and further into five subtypes according to the number of stalk cells, morphology and number of glandular head cells. Digitiform trichomes consist of one basal cell, one or two stalk cells and one apical secretory cell, which are of similar diameter and approximately equal length.",
journal = "Archives of Biological Sciences",
title = "Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill.",
number = "1",
volume = "65",
pages = "239-246",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_327"
}

Al Sheef Najat, B., Duletić-Laušević, S. N., Janošević, D., Budimir, S., Marin, M., Alimpić, A., Giweli, A.A.M.,& Marin, P.D.. (2013). Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill.. in Archives of Biological Sciences, 65(1), 239-246.
https://hdl.handle.net/21.15107/rcub_ibiss_327

Al Sheef Najat B, Duletić-Laušević SN, Janošević D, Budimir S, Marin M, Alimpić A, Giweli A, Marin P. Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill.. in Archives of Biological Sciences. 2013;65(1):239-246.
https://hdl.handle.net/21.15107/rcub_ibiss_327 .

Al Sheef Najat, Beleed, Duletić-Laušević, Sonja N., Janošević, Dušica, Budimir, Snežana, Marin, Marija, Alimpić, Ana, Giweli, A.A.M., Marin, P.D., "Micromorphology and ultrastructure of trichomes of Libyan Salvia fruticosa Mill." in Archives of Biological Sciences, 65, no. 1 (2013):239-246,
https://hdl.handle.net/21.15107/rcub_ibiss_327 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Nestorović Živković, Jasmina
AU  - Simonović, Ana
AU  - Maksimović, Vuk M
AU  - Budimir, Snežana
AU  - Janošević, Dušica A.
AU  - Đuricković, Milutin S
AU  - Nikolić, Miroslav
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1204
AB  - The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.
T2  - Plant Cell Tissue and Organ Culture
T1  - Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress
IS  - 3
VL  - 108
EP  - 400
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1204
ER  - 

@article{
author = "Mišić, Danijela and Šiler, Branislav and Nestorović Živković, Jasmina and Simonović, Ana and Maksimović, Vuk M and Budimir, Snežana and Janošević, Dušica A. and Đuricković, Milutin S and Nikolić, Miroslav",
year = "2012",
abstract = "The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress",
number = "3",
volume = "108",
pages = "400",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1204"
}

Mišić, D., Šiler, B., Nestorović Živković, J., Simonović, A., Maksimović, V. M., Budimir, S., Janošević, D. A., Đuricković, M. S.,& Nikolić, M.. (2012). Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture, 108(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1204

Mišić D, Šiler B, Nestorović Živković J, Simonović A, Maksimović VM, Budimir S, Janošević DA, Đuricković MS, Nikolić M. Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture. 2012;108(3):null-400.
https://hdl.handle.net/21.15107/rcub_ibiss_1204 .

Mišić, Danijela, Šiler, Branislav, Nestorović Živković, Jasmina, Simonović, Ana, Maksimović, Vuk M, Budimir, Snežana, Janošević, Dušica A., Đuricković, Milutin S, Nikolić, Miroslav, "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress" in Plant Cell Tissue and Organ Culture, 108, no. 3 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1204 .

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Milojević, Jelena
AU  - Kostić, Igor
AU  - Janošević, Dušica A.
AU  - Budimir, Snežana
AU  - Zdravković-Korać, Snežana
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1070
AB  - The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.
T2  - Hortscience
T1  - Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis
IS  - 12
VL  - 47
SP  - 79
EP  - 1744
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1070
ER  - 

@article{
author = "Ćalić, Dušica and Devrnja, Nina and Milojević, Jelena and Kostić, Igor and Janošević, Dušica A. and Budimir, Snežana and Zdravković-Korać, Snežana",
year = "2012",
abstract = "The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.",
journal = "Hortscience",
title = "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis",
number = "12",
volume = "47",
pages = "79-1744",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1070"
}

Ćalić, D., Devrnja, N., Milojević, J., Kostić, I., Janošević, D. A., Budimir, S.,& Zdravković-Korać, S.. (2012). Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience, 47(12), 79-1744.
https://hdl.handle.net/21.15107/rcub_ibiss_1070

Ćalić D, Devrnja N, Milojević J, Kostić I, Janošević DA, Budimir S, Zdravković-Korać S. Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience. 2012;47(12):79-1744.
https://hdl.handle.net/21.15107/rcub_ibiss_1070 .

Ćalić, Dušica, Devrnja, Nina, Milojević, Jelena, Kostić, Igor, Janošević, Dušica A., Budimir, Snežana, Zdravković-Korać, Snežana, "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis" in Hortscience, 47, no. 12 (2012):79-1744,
https://hdl.handle.net/21.15107/rcub_ibiss_1070 .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/308
AB  - Cytokinins are involved in plant cell proliferation leading to plant growth and morphogenesis. The size, activity and maintenance of the shoot apical meristem (SAM) are defined by a balanced rate of mitotic cell divisions and functional cell differentiation that are controlled by cytokinins. In order to investigate the effect of exogenous cytokinin on SAM, morpho-anatomical changes in the shoot apices of tobacco treated with benzyladenine (BA) were compared to those of untreated control plants.
T2  - Archives of Biological Sciences
T1  - Effect of cytokinins on shoot apical meristem in Nicotiana tabacum
IS  - 2
VL  - 64
SP  - 511
EP  - 516
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_308
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2012, 2012",
abstract = "Cytokinins are involved in plant cell proliferation leading to plant growth and morphogenesis. The size, activity and maintenance of the shoot apical meristem (SAM) are defined by a balanced rate of mitotic cell divisions and functional cell differentiation that are controlled by cytokinins. In order to investigate the effect of exogenous cytokinin on SAM, morpho-anatomical changes in the shoot apices of tobacco treated with benzyladenine (BA) were compared to those of untreated control plants.",
journal = "Archives of Biological Sciences",
title = "Effect of cytokinins on shoot apical meristem in Nicotiana tabacum",
number = "2",
volume = "64",
pages = "511-516",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_308"
}

Uzelac, B., Janošević, D., Stojičić, D.,& Budimir, S.. (2012). Effect of cytokinins on shoot apical meristem in Nicotiana tabacum. in Archives of Biological Sciences, 64(2), 511-516.
https://hdl.handle.net/21.15107/rcub_ibiss_308

Uzelac B, Janošević D, Stojičić D, Budimir S. Effect of cytokinins on shoot apical meristem in Nicotiana tabacum. in Archives of Biological Sciences. 2012;64(2):511-516.
https://hdl.handle.net/21.15107/rcub_ibiss_308 .

Uzelac, Branka, Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "Effect of cytokinins on shoot apical meristem in Nicotiana tabacum" in Archives of Biological Sciences, 64, no. 2 (2012):511-516,
https://hdl.handle.net/21.15107/rcub_ibiss_308 .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
AU  - Čokeša, V.
AU  - Budimir, Snežana
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/307
AB  - This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.
T2  - Archives of Biological Sciences
T1  - In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth
IS  - 2
VL  - 64
SP  - 503
EP  - 509
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_307
ER  - 

@article{
author = "Stojičić, Dragana and Janošević, Dušica and Uzelac, Branka and Čokeša, V. and Budimir, Snežana",
year = "2012, 2012",
abstract = "This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.",
journal = "Archives of Biological Sciences",
title = "In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth",
number = "2",
volume = "64",
pages = "503-509",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_307"
}

Stojičić, D., Janošević, D., Uzelac, B., Čokeša, V.,& Budimir, S.. (2012). In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth. in Archives of Biological Sciences, 64(2), 503-509.
https://hdl.handle.net/21.15107/rcub_ibiss_307

Stojičić D, Janošević D, Uzelac B, Čokeša V, Budimir S. In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth. in Archives of Biological Sciences. 2012;64(2):503-509.
https://hdl.handle.net/21.15107/rcub_ibiss_307 .

Stojičić, Dragana, Janošević, Dušica, Uzelac, Branka, Čokeša, V., Budimir, Snežana, "In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth" in Archives of Biological Sciences, 64, no. 2 (2012):503-509,
https://hdl.handle.net/21.15107/rcub_ibiss_307 .

TY  - JOUR
AU  - Stojicić, Dragana D
AU  - Janošević, Dušica A.
AU  - Uzelac, Branka
AU  - Cokesa, Vlado M
AU  - Budimir, Snežana
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1182
AB  - In Pinus peuce zygotic embryo culture grown on Gresshoff and Doy (1972; GD) basal medium, 2.22 mu M benzyladenine (BA) was superior in promoting adventitious bud induction during 4 weeks comparing to kinetin or BA + kinetin. Shoot elongation was achieved on half-strength GD medium devoid of plant growth regulators and containing activated charcoal. Pulse treatment with 1 mM indole-3-butyric acid (IBA) for 2 h, followed by transfer to half-strength GD medium, produced the most efficient rooting. Rooted shoots were transplanted to the greenhouse and plantlets continued to grow and developed into phenotypically normal plants. Up to 10 plants per explant can be obtained within 36 weeks from culture initiation.
T2  - Biologia Plantarum
T1  - Micropropagation of Pinus peuce
IS  - 2
VL  - 56
EP  - 364
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1182
ER  - 

@article{
author = "Stojicić, Dragana D and Janošević, Dušica A. and Uzelac, Branka and Cokesa, Vlado M and Budimir, Snežana",
year = "2012",
abstract = "In Pinus peuce zygotic embryo culture grown on Gresshoff and Doy (1972; GD) basal medium, 2.22 mu M benzyladenine (BA) was superior in promoting adventitious bud induction during 4 weeks comparing to kinetin or BA + kinetin. Shoot elongation was achieved on half-strength GD medium devoid of plant growth regulators and containing activated charcoal. Pulse treatment with 1 mM indole-3-butyric acid (IBA) for 2 h, followed by transfer to half-strength GD medium, produced the most efficient rooting. Rooted shoots were transplanted to the greenhouse and plantlets continued to grow and developed into phenotypically normal plants. Up to 10 plants per explant can be obtained within 36 weeks from culture initiation.",
journal = "Biologia Plantarum",
title = "Micropropagation of Pinus peuce",
number = "2",
volume = "56",
pages = "364",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1182"
}

Stojicić, D. D., Janošević, D. A., Uzelac, B., Cokesa, V. M.,& Budimir, S.. (2012). Micropropagation of Pinus peuce. in Biologia Plantarum, 56(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1182

Stojicić DD, Janošević DA, Uzelac B, Cokesa VM, Budimir S. Micropropagation of Pinus peuce. in Biologia Plantarum. 2012;56(2):null-364.
https://hdl.handle.net/21.15107/rcub_ibiss_1182 .

Stojicić, Dragana D, Janošević, Dušica A., Uzelac, Branka, Cokesa, Vlado M, Budimir, Snežana, "Micropropagation of Pinus peuce" in Biologia Plantarum, 56, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1182 .