TY  - JOUR
AU  - Pokimica, Nina
AU  - Ćosić, Tatjana
AU  - Uzelac, Branka
AU  - Ninković, Slavica
AU  - Raspor, Martin
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6631
AB  - Numerous biotechnological applications require a fast and efficient clonal propagation of whole plants under controlled laboratory conditions. For most plant species, the de novo regeneration of shoots from the cuttings of various plant organs can be obtained on nutrient media supplemented with plant hormones, auxin and cytokinin. While auxin is needed during the early stages of the process that include the establishment of pluripotent primordia and the subsequent acquisition of organogenic competence, cytokinin-supplemented media are required to induce these primordia to differentiate into developing shoots. The perception of cytokinin through the receptor ARABIDOPSIS HISTIDINE KINASE4 (AHK4) is crucial for the activation of the two main regulators of the establishment and maintenance of shoot apical meristems (SAMs): SHOOTMERISTEMLESS (STM) and the WUSCHEL-CLAVATA3 (WUS-CLV3) regulatory circuit. In this review, we summarize the current knowledge of the roles of the cytokinin signaling cascade in the perception and transduction of signals that are crucial for the de novo establishment of SAMs and lead to the desired biotechnological output—adventitious shoot multiplication. We highlight the functional differences between individual members of the multigene families involved in cytokinin signal transduction, and demonstrate how complex genetic regulation can be achieved through functional specialization of individual gene family members.
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation
IS  - 3
VL  - 14
DO  - 10.3390/biom14030381
SP  - 381
ER  - 

@article{
author = "Pokimica, Nina and Ćosić, Tatjana and Uzelac, Branka and Ninković, Slavica and Raspor, Martin",
year = "2024",
abstract = "Numerous biotechnological applications require a fast and efficient clonal propagation of whole plants under controlled laboratory conditions. For most plant species, the de novo regeneration of shoots from the cuttings of various plant organs can be obtained on nutrient media supplemented with plant hormones, auxin and cytokinin. While auxin is needed during the early stages of the process that include the establishment of pluripotent primordia and the subsequent acquisition of organogenic competence, cytokinin-supplemented media are required to induce these primordia to differentiate into developing shoots. The perception of cytokinin through the receptor ARABIDOPSIS HISTIDINE KINASE4 (AHK4) is crucial for the activation of the two main regulators of the establishment and maintenance of shoot apical meristems (SAMs): SHOOTMERISTEMLESS (STM) and the WUSCHEL-CLAVATA3 (WUS-CLV3) regulatory circuit. In this review, we summarize the current knowledge of the roles of the cytokinin signaling cascade in the perception and transduction of signals that are crucial for the de novo establishment of SAMs and lead to the desired biotechnological output—adventitious shoot multiplication. We highlight the functional differences between individual members of the multigene families involved in cytokinin signal transduction, and demonstrate how complex genetic regulation can be achieved through functional specialization of individual gene family members.",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation",
number = "3",
volume = "14",
doi = "10.3390/biom14030381",
pages = "381"
}

Pokimica, N., Ćosić, T., Uzelac, B., Ninković, S.,& Raspor, M.. (2024). Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation. in Biomolecules
Basel: MDPI., 14(3), 381.
https://doi.org/10.3390/biom14030381

Pokimica N, Ćosić T, Uzelac B, Ninković S, Raspor M. Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation. in Biomolecules. 2024;14(3):381.
doi:10.3390/biom14030381 .

Pokimica, Nina, Ćosić, Tatjana, Uzelac, Branka, Ninković, Slavica, Raspor, Martin, "Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation" in Biomolecules, 14, no. 3 (2024):381,
https://doi.org/10.3390/biom14030381 . .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
AU  - Čokeša, Vlado
AU  - Uzelac, Branka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6521
AB  - Pinus peuce (Macedonian pine) is considered a valuable ornamental tree that is frequently planted in parks and gardens, especially in Western Europe. This endemic pine is one of the most valuable conifer species in its native range, which currently consists of only two disjunct populations restricted to small mountainous areas of the Balkans and is listed as a near-threatened species. The reproduction of Macedonian pine by seed is limited, so in vitro propagation methods have emerged as a promising tool for large-scale propagation. The objective of this study was to develop an improved system for the micropropagation of P. peuce from juvenile plant material using a short-term liquid cytokinin pulse. For that, explants derived from 4-week-old seedlings were pulse-treated with different concentrations of N6-benzyladenine (BA) for 1 or 2 h to stimulate the induction of axillary buds. The highest axillary shoot formation was achieved with 222 µM BA pulse treatment, with an average number of ~six shoots per explant. Elongated shoots (≥10 mm) were detached from the explants and pulse-treated with 0.27 or 1.08 mM α-naphthaleneacetic acid (NAA) or 0.25 or 0.98 mM indole-3-butyric acid (IBA) for 1 or 2 h. IBA was more effective than NAA and led to a maximum rooting percentage (up to 40%) and the highest number of acclimatized plants (15–20%). Rooted plants were successfully transferred to ex vitro conditions.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Optimization of In Vitro Regeneration of Pinus peuce (Gris.)
IS  - 1
VL  - 10
DO  - 10.3390/horticulturae10010097
SP  - 97
ER  - 

@article{
author = "Stojičić, Dragana and Budimir, Snežana and Čokeša, Vlado and Uzelac, Branka",
year = "2024",
abstract = "Pinus peuce (Macedonian pine) is considered a valuable ornamental tree that is frequently planted in parks and gardens, especially in Western Europe. This endemic pine is one of the most valuable conifer species in its native range, which currently consists of only two disjunct populations restricted to small mountainous areas of the Balkans and is listed as a near-threatened species. The reproduction of Macedonian pine by seed is limited, so in vitro propagation methods have emerged as a promising tool for large-scale propagation. The objective of this study was to develop an improved system for the micropropagation of P. peuce from juvenile plant material using a short-term liquid cytokinin pulse. For that, explants derived from 4-week-old seedlings were pulse-treated with different concentrations of N6-benzyladenine (BA) for 1 or 2 h to stimulate the induction of axillary buds. The highest axillary shoot formation was achieved with 222 µM BA pulse treatment, with an average number of ~six shoots per explant. Elongated shoots (≥10 mm) were detached from the explants and pulse-treated with 0.27 or 1.08 mM α-naphthaleneacetic acid (NAA) or 0.25 or 0.98 mM indole-3-butyric acid (IBA) for 1 or 2 h. IBA was more effective than NAA and led to a maximum rooting percentage (up to 40%) and the highest number of acclimatized plants (15–20%). Rooted plants were successfully transferred to ex vitro conditions.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Optimization of In Vitro Regeneration of Pinus peuce (Gris.)",
number = "1",
volume = "10",
doi = "10.3390/horticulturae10010097",
pages = "97"
}

Stojičić, D., Budimir, S., Čokeša, V.,& Uzelac, B.. (2024). Optimization of In Vitro Regeneration of Pinus peuce (Gris.). in Horticulturae
Basel : MDPI., 10(1), 97.
https://doi.org/10.3390/horticulturae10010097

Stojičić D, Budimir S, Čokeša V, Uzelac B. Optimization of In Vitro Regeneration of Pinus peuce (Gris.). in Horticulturae. 2024;10(1):97.
doi:10.3390/horticulturae10010097 .

Stojičić, Dragana, Budimir, Snežana, Čokeša, Vlado, Uzelac, Branka, "Optimization of In Vitro Regeneration of Pinus peuce (Gris.)" in Horticulturae, 10, no. 1 (2024):97,
https://doi.org/10.3390/horticulturae10010097 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Jevremović, Slađana
AU  - Uzelac, Branka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6522
AB  - The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime
IS  - 1
VL  - 10
DO  - 10.3390/horticulturae10010017
SP  - 17
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Jevremović, Slađana and Uzelac, Branka",
year = "2024",
abstract = "The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime",
number = "1",
volume = "10",
doi = "10.3390/horticulturae10010017",
pages = "17"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Jevremović, S.,& Uzelac, B.. (2024). Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae
Basel : MDPI., 10(1), 17.
https://doi.org/10.3390/horticulturae10010017

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Jevremović S, Uzelac B. Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae. 2024;10(1):17.
doi:10.3390/horticulturae10010017 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Jevremović, Slađana, Uzelac, Branka, "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime" in Horticulturae, 10, no. 1 (2024):17,
https://doi.org/10.3390/horticulturae10010017 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Anđela, Tomić
AU  - Uzelac, Branka
AU  - Raspor, Martin
AU  - Pokimica, Nina
AU  - Devrnja, Nina
AU  - Ninković, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6111
AB  - Deposition of carbohydrates in the lower part of the stem represents a unique feature of kohlrabi (Brassica
oleracea var. gongylodes), a member of Brassicaceae family grown for its high nutritional value. Over the
past decade our team intensely investigated in vitro development and regeneration of this significant crop,
determining, among other specific characteristics, an intricate relation between sucrose, serving as a carbon
source for in vitro growth, and cytokinins (CKs), utilized for the induction of indirect de novo shoot
organogenesis (DNSO). Even though the formation of kohlrabi stem tuber in vitro has not yet been
successfully attained, development of an efficient in vitro system for kohlrabi growth and DNSO opened the
possibility to study sugar metabolism in young kohlrabi plants, including the effects of elevated sucrose
application (6% and 9%) and CKs (trans-zeatin, transZ; thidiazuron, TDZ). Expression analysis of ten
selected genes involved in sugar partitioning in plants, indicated that both sucrose and CKs had similar
influence in the sense of downregulating these genes compared to control grown on plant growth
regulator-free medium with 3% sucrose. TDZ application had the highest effect on relative expression of all
three sucrose synthase genes (SUS1/3/6), particularly in three week-old plantlets (S1), the developmental
stage characterized by callus formation in the CK-treated kohlrabi. This trend was also observed for the
CWINV1, coding for β-fructofuranosidase and UGP1, coding for UTP--glucose-1-phosphate uridylyltransferase
1. On the other hand, high concentration of sucrose induced stronger downregulation of CINV1 (alkaline/
neutral invertase1), HXK1 (hexokinase1), GPI (glucose-6-phosphate isomerase) and SPS1 (sucrose-phosphate
synthase 1). TransZ led to small changes in gene expression, and even led to upregulation of SUS1 and
CINV1. Older plantlets (S2, four week-old, forming de novo shoots on CK treatment) were generally less
affected by applied treatments. In addition, histological sections of treated kohlrabi hypocotyls revealed
distinct changes at morpho-anatomical level. Our results, for the first time, depict effects of sucrose and CK
on activity of soluble sugars-related gene in young kohlrabi, suggesting an altered metabolism and transport
of soluble sugars, providing a starting point to the elucidation of stem tuber development and sugar
partitioning in kohlrabi.
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi
SP  - 345
EP  - 345
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6111
ER  - 

@conference{
author = "Ćosić, Tatjana and Anđela, Tomić and Uzelac, Branka and Raspor, Martin and Pokimica, Nina and Devrnja, Nina and Ninković, Slavica",
year = "2023",
abstract = "Deposition of carbohydrates in the lower part of the stem represents a unique feature of kohlrabi (Brassica
oleracea var. gongylodes), a member of Brassicaceae family grown for its high nutritional value. Over the
past decade our team intensely investigated in vitro development and regeneration of this significant crop,
determining, among other specific characteristics, an intricate relation between sucrose, serving as a carbon
source for in vitro growth, and cytokinins (CKs), utilized for the induction of indirect de novo shoot
organogenesis (DNSO). Even though the formation of kohlrabi stem tuber in vitro has not yet been
successfully attained, development of an efficient in vitro system for kohlrabi growth and DNSO opened the
possibility to study sugar metabolism in young kohlrabi plants, including the effects of elevated sucrose
application (6% and 9%) and CKs (trans-zeatin, transZ; thidiazuron, TDZ). Expression analysis of ten
selected genes involved in sugar partitioning in plants, indicated that both sucrose and CKs had similar
influence in the sense of downregulating these genes compared to control grown on plant growth
regulator-free medium with 3% sucrose. TDZ application had the highest effect on relative expression of all
three sucrose synthase genes (SUS1/3/6), particularly in three week-old plantlets (S1), the developmental
stage characterized by callus formation in the CK-treated kohlrabi. This trend was also observed for the
CWINV1, coding for β-fructofuranosidase and UGP1, coding for UTP--glucose-1-phosphate uridylyltransferase
1. On the other hand, high concentration of sucrose induced stronger downregulation of CINV1 (alkaline/
neutral invertase1), HXK1 (hexokinase1), GPI (glucose-6-phosphate isomerase) and SPS1 (sucrose-phosphate
synthase 1). TransZ led to small changes in gene expression, and even led to upregulation of SUS1 and
CINV1. Older plantlets (S2, four week-old, forming de novo shoots on CK treatment) were generally less
affected by applied treatments. In addition, histological sections of treated kohlrabi hypocotyls revealed
distinct changes at morpho-anatomical level. Our results, for the first time, depict effects of sucrose and CK
on activity of soluble sugars-related gene in young kohlrabi, suggesting an altered metabolism and transport
of soluble sugars, providing a starting point to the elucidation of stem tuber development and sugar
partitioning in kohlrabi.",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi",
pages = "345-345",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6111"
}

Ćosić, T., Anđela, T., Uzelac, B., Raspor, M., Pokimica, N., Devrnja, N.,& Ninković, S.. (2023). Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 345-345.
https://hdl.handle.net/21.15107/rcub_ibiss_6111

Ćosić T, Anđela T, Uzelac B, Raspor M, Pokimica N, Devrnja N, Ninković S. Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:345-345.
https://hdl.handle.net/21.15107/rcub_ibiss_6111 .

Ćosić, Tatjana, Anđela, Tomić, Uzelac, Branka, Raspor, Martin, Pokimica, Nina, Devrnja, Nina, Ninković, Slavica, "Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):345-345,
https://hdl.handle.net/21.15107/rcub_ibiss_6111 .

TY  - JOUR
AU  - Popržen, Tatjana
AU  - Nikolić, Ivan
AU  - Krstić-Milošević, Dijana
AU  - Uzelac, Branka
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
AU  - Radulović, Olga
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6442
AB  - The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).
PB  - Basel : MDPI
T2  - International Journal of Molecular Sciences
T1  - Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)
IS  - 24
VL  - 24
DO  - 10.3390/ijms242417207
SP  - 17207
ER  - 

@article{
author = "Popržen, Tatjana and Nikolić, Ivan and Krstić-Milošević, Dijana and Uzelac, Branka and Trifunović-Momčilov, Milana and Marković, Marija and Radulović, Olga",
year = "2023",
abstract = "The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).",
publisher = "Basel : MDPI",
journal = "International Journal of Molecular Sciences",
title = "Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)",
number = "24",
volume = "24",
doi = "10.3390/ijms242417207",
pages = "17207"
}

Popržen, T., Nikolić, I., Krstić-Milošević, D., Uzelac, B., Trifunović-Momčilov, M., Marković, M.,& Radulović, O.. (2023). Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.). in International Journal of Molecular Sciences
Basel : MDPI., 24(24), 17207.
https://doi.org/10.3390/ijms242417207

Popržen T, Nikolić I, Krstić-Milošević D, Uzelac B, Trifunović-Momčilov M, Marković M, Radulović O. Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.). in International Journal of Molecular Sciences. 2023;24(24):17207.
doi:10.3390/ijms242417207 .

Popržen, Tatjana, Nikolić, Ivan, Krstić-Milošević, Dijana, Uzelac, Branka, Trifunović-Momčilov, Milana, Marković, Marija, Radulović, Olga, "Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)" in International Journal of Molecular Sciences, 24, no. 24 (2023):17207,
https://doi.org/10.3390/ijms242417207 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Radulović, Olga
AU  - Subotić, Angelina
AU  - Uzelac, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6135
AB  - Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H2O2, total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H2O2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana. The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.
PB  - Basel: MDPI
T2  - Agriculture
T1  - Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?
IS  - 10
VL  - 13
DO  - 10.3390/agriculture13101903
SP  - 1903
ER  - 

@article{
author = "Đurić, Marija and Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Radulović, Olga and Subotić, Angelina and Uzelac, Branka",
year = "2023",
abstract = "Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H2O2, total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H2O2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana. The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.",
publisher = "Basel: MDPI",
journal = "Agriculture",
title = "Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?",
number = "10",
volume = "13",
doi = "10.3390/agriculture13101903",
pages = "1903"
}

Đurić, M., Trifunović-Momčilov, M., Milošević, S., Marković, M., Radulović, O., Subotić, A.,& Uzelac, B.. (2023). Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?. in Agriculture
Basel: MDPI., 13(10), 1903.
https://doi.org/10.3390/agriculture13101903

Đurić M, Trifunović-Momčilov M, Milošević S, Marković M, Radulović O, Subotić A, Uzelac B. Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?. in Agriculture. 2023;13(10):1903.
doi:10.3390/agriculture13101903 .

Đurić, Marija, Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Radulović, Olga, Subotić, Angelina, Uzelac, Branka, "Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?" in Agriculture, 13, no. 10 (2023):1903,
https://doi.org/10.3390/agriculture13101903 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Uzelac, Branka
AU  - Subotić, Angelina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6089
AB  - Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro
IS  - 8
VL  - 9
DO  - 10.3390/horticulturae9080910
SP  - 910
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Uzelac, Branka and Subotić, Angelina",
year = "2023",
abstract = "Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro",
number = "8",
volume = "9",
doi = "10.3390/horticulturae9080910",
pages = "910"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Uzelac, B.,& Subotić, A.. (2023). The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro. in Horticulturae
Basel : MDPI., 9(8), 910.
https://doi.org/10.3390/horticulturae9080910

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Uzelac B, Subotić A. The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro. in Horticulturae. 2023;9(8):910.
doi:10.3390/horticulturae9080910 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Uzelac, Branka, Subotić, Angelina, "The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro" in Horticulturae, 9, no. 8 (2023):910,
https://doi.org/10.3390/horticulturae9080910 . .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Stojanović, Gordana
AU  - Zlatković, Bojan
AU  - Jovanović, Snežana
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/2/198
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8778064
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4768
AB  - Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography-mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.
IS  - 2
VL  - 11
DO  - 10.3390/plants11020198
SP  - 198
ER  - 

@article{
author = "Stojičić, Dragana and Tošić, Svetlana and Stojanović, Gordana and Zlatković, Bojan and Jovanović, Snežana and Budimir, Snežana and Uzelac, Branka",
year = "2022",
abstract = "Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography-mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.",
number = "2",
volume = "11",
doi = "10.3390/plants11020198",
pages = "198"
}

Stojičić, D., Tošić, S., Stojanović, G., Zlatković, B., Jovanović, S., Budimir, S.,& Uzelac, B.. (2022). Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.. in Plants (Basel, Switzerland)
Basel: MDPI., 11(2), 198.
https://doi.org/10.3390/plants11020198

Stojičić D, Tošić S, Stojanović G, Zlatković B, Jovanović S, Budimir S, Uzelac B. Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.. in Plants (Basel, Switzerland). 2022;11(2):198.
doi:10.3390/plants11020198 .

Stojičić, Dragana, Tošić, Svetlana, Stojanović, Gordana, Zlatković, Bojan, Jovanović, Snežana, Budimir, Snežana, Uzelac, Branka, "Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source." in Plants (Basel, Switzerland), 11, no. 2 (2022):198,
https://doi.org/10.3390/plants11020198 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Budimir, Snežana
AU  - Stojičić, Dragana
PY  - 2022
UR  - https://journal.pmf.ni.ac.rs/bionys/index.php/bionys/article/view/477
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5661
AB  - Many of the species belonging to the Lamiaceae family are considered aromatic
plants due to the presence of glandular trichomes, which have a distinct ability
to synthesize, secrete or store large amounts of specialized metabolites that
play a crucial role in mediating the plant–environment interactions. These
compounds often have marked bioactive properties, rendering a commercial
value to the plants that produce them. A number of biological effects have
been associated with the main monoterpenoids detected in investigated
Micromeria spp. and Clinopodium spp. essential oils. One alternative for the
production of these bioactive metabolites is in vitro plant tissue culture. The
present study was initiated to investigate the effects of in vitro culture on
the secretion of leaf glandular trichomes, the main structures involved in the
essential oil production. The glandular indumentum was studied by means of
light microscopy and scanning electron microscopy in an attempt to correlate
the phytochemical traits with the glandular trichome morphotypes of selected
Lamiaceae species.
PB  - Niš: University of Niš
T2  - Biologica Nyssana
T1  - Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach
IS  - 2
VL  - 13
DO  - 10.5281/zenodo.7476250
SP  - 191
EP  - 203
ER  - 

@article{
author = "Uzelac, Branka and Budimir, Snežana and Stojičić, Dragana",
year = "2022",
abstract = "Many of the species belonging to the Lamiaceae family are considered aromatic
plants due to the presence of glandular trichomes, which have a distinct ability
to synthesize, secrete or store large amounts of specialized metabolites that
play a crucial role in mediating the plant–environment interactions. These
compounds often have marked bioactive properties, rendering a commercial
value to the plants that produce them. A number of biological effects have
been associated with the main monoterpenoids detected in investigated
Micromeria spp. and Clinopodium spp. essential oils. One alternative for the
production of these bioactive metabolites is in vitro plant tissue culture. The
present study was initiated to investigate the effects of in vitro culture on
the secretion of leaf glandular trichomes, the main structures involved in the
essential oil production. The glandular indumentum was studied by means of
light microscopy and scanning electron microscopy in an attempt to correlate
the phytochemical traits with the glandular trichome morphotypes of selected
Lamiaceae species.",
publisher = "Niš: University of Niš",
journal = "Biologica Nyssana",
title = "Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach",
number = "2",
volume = "13",
doi = "10.5281/zenodo.7476250",
pages = "191-203"
}

Uzelac, B., Budimir, S.,& Stojičić, D.. (2022). Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach. in Biologica Nyssana
Niš: University of Niš., 13(2), 191-203.
https://doi.org/10.5281/zenodo.7476250

Uzelac B, Budimir S, Stojičić D. Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach. in Biologica Nyssana. 2022;13(2):191-203.
doi:10.5281/zenodo.7476250 .

Uzelac, Branka, Budimir, Snežana, Stojičić, Dragana, "Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach" in Biologica Nyssana, 13, no. 2 (2022):191-203,
https://doi.org/10.5281/zenodo.7476250 . .

TY  - CONF
AU  - Uzelac, Branka
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
AU  - Tošić, Svetlana
AU  - Zlatković, Bojan
AU  - Blagojević, Saša
AU  - Manić, Branislav
AU  - Janjanin, Mirjana
AU  - Slavkovska, Violeta
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5664
AB  - Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.
PB  - Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu
C3  - Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
T1  - Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach
DO  - 10.46793/SBT27.345U
SP  - 345
EP  - 350
ER  - 

@conference{
author = "Uzelac, Branka and Stojičić, Dragana and Budimir, Snežana and Tošić, Svetlana and Zlatković, Bojan and Blagojević, Saša and Manić, Branislav and Janjanin, Mirjana and Slavkovska, Violeta",
year = "2022",
abstract = "Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.",
publisher = "Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu",
journal = "Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia",
title = "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach",
doi = "10.46793/SBT27.345U",
pages = "345-350"
}

Uzelac, B., Stojičić, D., Budimir, S., Tošić, S., Zlatković, B., Blagojević, S., Manić, B., Janjanin, M.,& Slavkovska, V.. (2022). Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu., 345-350.
https://doi.org/10.46793/SBT27.345U

Uzelac B, Stojičić D, Budimir S, Tošić S, Zlatković B, Blagojević S, Manić B, Janjanin M, Slavkovska V. Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia. 2022;:345-350.
doi:10.46793/SBT27.345U .

Uzelac, Branka, Stojičić, Dragana, Budimir, Snežana, Tošić, Svetlana, Zlatković, Bojan, Blagojević, Saša, Manić, Branislav, Janjanin, Mirjana, Slavkovska, Violeta, "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach" in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia (2022):345-350,
https://doi.org/10.46793/SBT27.345U . .

TY  - CONF
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Zlatković, Bojan
AU  - Blagojević, Saša
AU  - Manić, Branislav
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5662
AB  - Clinopodium thymifolium (Scop.) Kuntze (syn. Micromeria thymifolia (Scop.) Fritsch) is a Balkan endemic species, traditionally used as a condiment and medicinal plant in the Mediterranean area. Clinopodium species were shown to produce considerable quantities (> 0.5%) of essential oils. The essential oil of wild-growing C. thymifolium possesses high antimicrobial activity. One alternative for the production of commercially important plant-derived metabolites, especially of rare plant genotypes, is in vitro plant tissue culture, given that the metabolic potential of in vitro shoot cultures can be manipulated by varying in vitro culture conditions. In view of the potential pharmacological and commercial value of C. thymifolium, the present study was initiated to propagate this aromatic plant species with the aim to increase the accumulation of biomass and the production of secondary metabolites. Shoots of wild-growing plants, dissected into one-node stem segments bearing two axillary buds, were used to establish in vitro cultures. Shoot multiplication was carried out on basal nutritional medium and on medium supplemented with various plant growth regulators at different concentrations. Nodal segments developed axillary shoots, and were successfully propagated on basal medium, whereas tested plant growth regulators differently affected their multiplication and biomass production.
PB  - Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
C3  - Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
T1  - Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5662
ER  - 

@conference{
editor = "Ranđelović, Vladimir, Stojanović-Radić, Zorica, Nikolić, Danijela, Jenačković Gocić, Dragana",
author = "Stojičić, Dragana and Tošić, Svetlana and Zlatković, Bojan and Blagojević, Saša and Manić, Branislav and Budimir, Snežana and Uzelac, Branka",
year = "2022",
abstract = "Clinopodium thymifolium (Scop.) Kuntze (syn. Micromeria thymifolia (Scop.) Fritsch) is a Balkan endemic species, traditionally used as a condiment and medicinal plant in the Mediterranean area. Clinopodium species were shown to produce considerable quantities (> 0.5%) of essential oils. The essential oil of wild-growing C. thymifolium possesses high antimicrobial activity. One alternative for the production of commercially important plant-derived metabolites, especially of rare plant genotypes, is in vitro plant tissue culture, given that the metabolic potential of in vitro shoot cultures can be manipulated by varying in vitro culture conditions. In view of the potential pharmacological and commercial value of C. thymifolium, the present study was initiated to propagate this aromatic plant species with the aim to increase the accumulation of biomass and the production of secondary metabolites. Shoots of wild-growing plants, dissected into one-node stem segments bearing two axillary buds, were used to establish in vitro cultures. Shoot multiplication was carried out on basal nutritional medium and on medium supplemented with various plant growth regulators at different concentrations. Nodal segments developed axillary shoots, and were successfully propagated on basal medium, whereas tested plant growth regulators differently affected their multiplication and biomass production.",
publisher = "Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš",
journal = "Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia",
title = "Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)",
pages = "113",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5662"
}

Ranđelović, V., Stojanović-Radić, Z., Nikolić, D., Jenačković Gocić, D., Stojičić, D., Tošić, S., Zlatković, B., Blagojević, S., Manić, B., Budimir, S.,& Uzelac, B.. (2022). Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae). in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 113.
https://hdl.handle.net/21.15107/rcub_ibiss_5662

Ranđelović V, Stojanović-Radić Z, Nikolić D, Jenačković Gocić D, Stojičić D, Tošić S, Zlatković B, Blagojević S, Manić B, Budimir S, Uzelac B. Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae). in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia. 2022;:113.
https://hdl.handle.net/21.15107/rcub_ibiss_5662 .

Ranđelović, Vladimir, Stojanović-Radić, Zorica, Nikolić, Danijela, Jenačković Gocić, Dragana, Stojičić, Dragana, Tošić, Svetlana, Zlatković, Bojan, Blagojević, Saša, Manić, Branislav, Budimir, Snežana, Uzelac, Branka, "Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)" in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia (2022):113,
https://hdl.handle.net/21.15107/rcub_ibiss_5662 .

TY  - CONF
AU  - Uzelac, Branka
AU  - Budimir, Snežana
AU  - Stojičić, Dragana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5663
AB  - Many of the species belonging to the Lamiaceae family are considered aromatic plants due to the presence of glandular trichomes, which have a distinct ability to synthesize, secrete or store large amounts of specialized metabolites that play a crucial role in mediating the plant–environment interactions. Secondary metabolites are biosynthesized as a defensive strategy of plants in response to natural perturbations, thus enabling them to adapt to the environmental stresses typical of each individual ecological niche. These compounds often have marked bioactive properties, rendering a commercial value to the plants that produce them. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these bioactive metabolites is in vitro plant tissue culture. The present study was initiated to investigate the effects of in vitro culture on the secretion of leaf glandular trichomes, the main structures involved in the essential oil production. The glandular indumentum was studied by means of light microscopy and scanning electron microscopy in an attempt to correlate the phytochemical traits with the glandular trichome morphotypes of selected Lamiaceae species.
PB  - Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
C3  - Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
T1  - Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach
SP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5663
ER  - 

@conference{
author = "Uzelac, Branka and Budimir, Snežana and Stojičić, Dragana",
year = "2022",
abstract = "Many of the species belonging to the Lamiaceae family are considered aromatic plants due to the presence of glandular trichomes, which have a distinct ability to synthesize, secrete or store large amounts of specialized metabolites that play a crucial role in mediating the plant–environment interactions. Secondary metabolites are biosynthesized as a defensive strategy of plants in response to natural perturbations, thus enabling them to adapt to the environmental stresses typical of each individual ecological niche. These compounds often have marked bioactive properties, rendering a commercial value to the plants that produce them. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these bioactive metabolites is in vitro plant tissue culture. The present study was initiated to investigate the effects of in vitro culture on the secretion of leaf glandular trichomes, the main structures involved in the essential oil production. The glandular indumentum was studied by means of light microscopy and scanning electron microscopy in an attempt to correlate the phytochemical traits with the glandular trichome morphotypes of selected Lamiaceae species.",
publisher = "Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš",
journal = "Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia",
title = "Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach",
pages = "120",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5663"
}

Uzelac, B., Budimir, S.,& Stojičić, D.. (2022). Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach. in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 120.
https://hdl.handle.net/21.15107/rcub_ibiss_5663

Uzelac B, Budimir S, Stojičić D. Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach. in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia. 2022;:120.
https://hdl.handle.net/21.15107/rcub_ibiss_5663 .

Uzelac, Branka, Budimir, Snežana, Stojičić, Dragana, "Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach" in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia (2022):120,
https://hdl.handle.net/21.15107/rcub_ibiss_5663 .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2021
UR  - https://doi.org/10.3390/plants10050902
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4228
AB  - In ornamental geophytes, conventional vegetative propagation is not economically feasible due to very slow development and ineffective methods. It can take several years until a new plant is formed and commercial profitability is achieved. Therefore, micropropagation techniques have been developed to increase the multiplication rate and thus shorten the multiplication and regeneration period. The majority of these techniques rely on the formation of new bulbs and their sprouting. Dormancy is one of the main limiting factors to speed up multiplication in vitro. Bulbous species have a period of bulb dormancy which enables them to survive unfavorable natural conditions. Bulbs grown in vitro also exhibit dormancy, which has to be overcome in order to allow sprouting of bulbs in the next vegetation period. During the period of dormancy, numerous physiological processes occur, many of which have not been elucidated yet. Understanding the process of dormancy will allow us to speed up and improve breeding of geophytes and thereby achieve economic profitability, which is very important for horticulture. This review focuses on recent findings in the area of bulb dormancy initiation and release in fritillaries, with particular emphasis on the effect of plant growth regulators and low-temperature pretreatment on dormancy release in relation to induction of antioxidative enzymes’ activity in vitro.
PB  - MDPI AG
T2  - Plants
T1  - Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters
IS  - 5
VL  - 10
DO  - 10.3390/plants10050902
SP  - 902
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Jevremović, Slađana and Subotić, Angelina",
year = "2021",
abstract = "In ornamental geophytes, conventional vegetative propagation is not economically feasible due to very slow development and ineffective methods. It can take several years until a new plant is formed and commercial profitability is achieved. Therefore, micropropagation techniques have been developed to increase the multiplication rate and thus shorten the multiplication and regeneration period. The majority of these techniques rely on the formation of new bulbs and their sprouting. Dormancy is one of the main limiting factors to speed up multiplication in vitro. Bulbous species have a period of bulb dormancy which enables them to survive unfavorable natural conditions. Bulbs grown in vitro also exhibit dormancy, which has to be overcome in order to allow sprouting of bulbs in the next vegetation period. During the period of dormancy, numerous physiological processes occur, many of which have not been elucidated yet. Understanding the process of dormancy will allow us to speed up and improve breeding of geophytes and thereby achieve economic profitability, which is very important for horticulture. This review focuses on recent findings in the area of bulb dormancy initiation and release in fritillaries, with particular emphasis on the effect of plant growth regulators and low-temperature pretreatment on dormancy release in relation to induction of antioxidative enzymes’ activity in vitro.",
publisher = "MDPI AG",
journal = "Plants",
title = "Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters",
number = "5",
volume = "10",
doi = "10.3390/plants10050902",
pages = "902"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Jevremović, S.,& Subotić, A.. (2021). Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters. in Plants
MDPI AG., 10(5), 902.
https://doi.org/10.3390/plants10050902

Marković M, Trifunović-Momčilov M, Uzelac B, Jevremović S, Subotić A. Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters. in Plants. 2021;10(5):902.
doi:10.3390/plants10050902 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Jevremović, Slađana, Subotić, Angelina, "Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters" in Plants, 10, no. 5 (2021):902,
https://doi.org/10.3390/plants10050902 . .

TY  - CHAP
AU  - Uzelac, Branka
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2020
UR  - https://link.springer.com/referenceworkentry/10.1007/978-3-030-11253-0_1-1
UR  - http://link.springer.com/10.1007/978-3-030-11253-0_1-1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3729
AB  - Glandular trichomes found on the surface of many higher plants contain specialized cells that produce and secrete copious amounts of particular secretory products. Leaf glandular trichomes of the non-model plant species Nicotiana tabacum represent a biologically active and stress-responsive tissue that contributes to plant defense response against biotic and abiotic stress and also influences leaf aroma and smoke flavor. Two morphologically different types of tobacco capitate trichomes, long- and short-stalked, with distinct functions, display ultrastructural features that are common to terpene-secreting glands, but only the secretory cells of the tall glandular trichomes are considered to be the site of biosynthesis of certain exudate compounds, including diterpenes and sucrose esters. Ultrastructural and histochemical characterization of tall glandular trichomes is described in an attempt to understand the contribution of these glands to the total secretion produced. Possible roles of distinct cellular compartments involved in the secretory process and secondary metabolite secretion under in vitro conditions are discussed.
PB  - Cham: Springer
T2  - Plant Cell and Tissue Differentiation and Secondary Metabolites
T1  - Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites
DO  - 10.1007/978-3-030-11253-0_1-1
ER  - 

@inbook{
author = "Uzelac, Branka and Stojičić, Dragana and Budimir, Snežana",
year = "2020",
abstract = "Glandular trichomes found on the surface of many higher plants contain specialized cells that produce and secrete copious amounts of particular secretory products. Leaf glandular trichomes of the non-model plant species Nicotiana tabacum represent a biologically active and stress-responsive tissue that contributes to plant defense response against biotic and abiotic stress and also influences leaf aroma and smoke flavor. Two morphologically different types of tobacco capitate trichomes, long- and short-stalked, with distinct functions, display ultrastructural features that are common to terpene-secreting glands, but only the secretory cells of the tall glandular trichomes are considered to be the site of biosynthesis of certain exudate compounds, including diterpenes and sucrose esters. Ultrastructural and histochemical characterization of tall glandular trichomes is described in an attempt to understand the contribution of these glands to the total secretion produced. Possible roles of distinct cellular compartments involved in the secretory process and secondary metabolite secretion under in vitro conditions are discussed.",
publisher = "Cham: Springer",
journal = "Plant Cell and Tissue Differentiation and Secondary Metabolites",
booktitle = "Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites",
doi = "10.1007/978-3-030-11253-0_1-1"
}

Uzelac, B., Stojičić, D.,& Budimir, S.. (2020). Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites. in Plant Cell and Tissue Differentiation and Secondary Metabolites
Cham: Springer..
https://doi.org/10.1007/978-3-030-11253-0_1-1

Uzelac B, Stojičić D, Budimir S. Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites. in Plant Cell and Tissue Differentiation and Secondary Metabolites. 2020;.
doi:10.1007/978-3-030-11253-0_1-1 .

Uzelac, Branka, Stojičić, Dragana, Budimir, Snežana, "Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites" in Plant Cell and Tissue Differentiation and Secondary Metabolites (2020),
https://doi.org/10.1007/978-3-030-11253-0_1-1 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Radulović, Olga
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1573
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4010
AB  - The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.
PB  - MDPI AG
T2  - Plants
T1  - Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs
IS  - 11
VL  - 9
DO  - 10.3390/plants9111573
SP  - 1573
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Radulović, Olga and Milošević, Snežana and Jevremović, Slađana and Subotić, Angelina",
year = "2020",
abstract = "The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.",
publisher = "MDPI AG",
journal = "Plants",
title = "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs",
number = "11",
volume = "9",
doi = "10.3390/plants9111573",
pages = "1573"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Radulović, O., Milošević, S., Jevremović, S.,& Subotić, A.. (2020). Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. in Plants
MDPI AG., 9(11), 1573.
https://doi.org/10.3390/plants9111573

Marković M, Trifunović-Momčilov M, Uzelac B, Radulović O, Milošević S, Jevremović S, Subotić A. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. in Plants. 2020;9(11):1573.
doi:10.3390/plants9111573 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Radulović, Olga, Milošević, Snežana, Jevremović, Slađana, Subotić, Angelina, "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs" in Plants, 9, no. 11 (2020):1573,
https://doi.org/10.3390/plants9111573 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Cingel, Aleksandar
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1449
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3996
AB  - Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors-ancymidol (A) and paclobutrazol (P)-the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb's dormancy and its sprouting.
PB  - MDPI AG
T2  - Plants (Basel, Switzerland)
T1  - Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.
IS  - 11
VL  - 9
DO  - 10.3390/plants9111449
SP  - 1449
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Cingel, Aleksandar and Milošević, Snežana and Jevremović, Slađana and Subotić, Angelina",
year = "2020",
abstract = "Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors-ancymidol (A) and paclobutrazol (P)-the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb's dormancy and its sprouting.",
publisher = "MDPI AG",
journal = "Plants (Basel, Switzerland)",
title = "Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.",
number = "11",
volume = "9",
doi = "10.3390/plants9111449",
pages = "1449"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Cingel, A., Milošević, S., Jevremović, S.,& Subotić, A.. (2020). Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.. in Plants (Basel, Switzerland)
MDPI AG., 9(11), 1449.
https://doi.org/10.3390/plants9111449

Marković M, Trifunović-Momčilov M, Uzelac B, Cingel A, Milošević S, Jevremović S, Subotić A. Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.. in Plants (Basel, Switzerland). 2020;9(11):1449.
doi:10.3390/plants9111449 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Cingel, Aleksandar, Milošević, Snežana, Jevremović, Slađana, Subotić, Angelina, "Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content." in Plants (Basel, Switzerland), 9, no. 11 (2020):1449,
https://doi.org/10.3390/plants9111449 . .

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Uzelac, Branka
AU  - Ćalić, Dušica
AU  - Pavlović, Suzana
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3997
AB  - High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.
PB  - Nature Research
T2  - Scientific Reports
T1  - Variability in somatic embryo-forming capacity of spinach
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-76279-9
SP  - 19290
ER  - 

@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Uzelac, Branka and Ćalić, Dušica and Pavlović, Suzana and Milojević, Jelena",
year = "2020",
abstract = "High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Variability in somatic embryo-forming capacity of spinach",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-76279-9",
pages = "19290"
}

Belić, M., Zdravković-Korać, S., Uzelac, B., Ćalić, D., Pavlović, S.,& Milojević, J.. (2020). Variability in somatic embryo-forming capacity of spinach. in Scientific Reports
Nature Research., 10(1), 19290.
https://doi.org/10.1038/s41598-020-76279-9

Belić M, Zdravković-Korać S, Uzelac B, Ćalić D, Pavlović S, Milojević J. Variability in somatic embryo-forming capacity of spinach. in Scientific Reports. 2020;10(1):19290.
doi:10.1038/s41598-020-76279-9 .

Belić, Maja, Zdravković-Korać, Snežana, Uzelac, Branka, Ćalić, Dušica, Pavlović, Suzana, Milojević, Jelena, "Variability in somatic embryo-forming capacity of spinach" in Scientific Reports, 10, no. 1 (2020):19290,
https://doi.org/10.1038/s41598-020-76279-9 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Uzelac, Branka
AU  - Tadić, Vojin
AU  - Trifunović-Momčilov, Milana
AU  - Lozo, Jelena
AU  - Marković, Marija
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/5/599
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3676
AB  - The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.
PB  - MDPI AG
T2  - Plants
T1  - Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations
IS  - 5
VL  - 9
DO  - 10.3390/plants9050599
SP  - 599
ER  - 

@article{
author = "Radulović, Olga and Stanković, Slaviša and Uzelac, Branka and Tadić, Vojin and Trifunović-Momčilov, Milana and Lozo, Jelena and Marković, Marija",
year = "2020",
abstract = "The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.",
publisher = "MDPI AG",
journal = "Plants",
title = "Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations",
number = "5",
volume = "9",
doi = "10.3390/plants9050599",
pages = "599"
}

Radulović, O., Stanković, S., Uzelac, B., Tadić, V., Trifunović-Momčilov, M., Lozo, J.,& Marković, M.. (2020). Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. in Plants
MDPI AG., 9(5), 599.
https://doi.org/10.3390/plants9050599

Radulović O, Stanković S, Uzelac B, Tadić V, Trifunović-Momčilov M, Lozo J, Marković M. Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. in Plants. 2020;9(5):599.
doi:10.3390/plants9050599 .

Radulović, Olga, Stanković, Slaviša, Uzelac, Branka, Tadić, Vojin, Trifunović-Momčilov, Milana, Lozo, Jelena, Marković, Marija, "Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations" in Plants, 9, no. 5 (2020):599,
https://doi.org/10.3390/plants9050599 . .

TY  - CONF
AU  - Janjanin, Mirjana
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6719
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia
T1  - Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants
DO  - 978-86-80335-11-7
SP  - 154
EP  - 156
ER  - 

@conference{
author = "Janjanin, Mirjana and Tošić, Svetlana and Stojičić, Dragana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia",
title = "Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants",
doi = "978-86-80335-11-7",
pages = "154-156"
}

Janjanin, M., Tošić, S., Stojičić, D., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants. in Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 154-156.
https://doi.org/978-86-80335-11-7

Janjanin M, Tošić S, Stojičić D, Zlatković B, Budimir S, Uzelac B. Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants. in Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia. 2019;:154-156.
doi:978-86-80335-11-7 .

Janjanin, Mirjana, Tošić, Svetlana, Stojičić, Dragana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants" in Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia (2019):154-156,
https://doi.org/978-86-80335-11-7 . .

TY  - JOUR
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Slavkovska, Violeta
AU  - Mihailov-Krstev, Tatjana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - http://link.springer.com/10.1007/s00425-018-03071-5
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3256
AB  - MAIN CONCLUSION In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.
PB  - Springer Berlin Heidelberg
T2  - Planta
T1  - Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).
DO  - 10.1007/s00425-018-03071-5
ER  - 

@article{
author = "Tošić, Svetlana and Stojičić, Dragana and Slavkovska, Violeta and Mihailov-Krstev, Tatjana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
abstract = "MAIN CONCLUSION In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.",
publisher = "Springer Berlin Heidelberg",
journal = "Planta",
title = "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).",
doi = "10.1007/s00425-018-03071-5"
}

Tošić, S., Stojičić, D., Slavkovska, V., Mihailov-Krstev, T., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).. in Planta
Springer Berlin Heidelberg..
https://doi.org/10.1007/s00425-018-03071-5

Tošić S, Stojičić D, Slavkovska V, Mihailov-Krstev T, Zlatković B, Budimir S, Uzelac B. Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).. in Planta. 2019;.
doi:10.1007/s00425-018-03071-5 .

Tošić, Svetlana, Stojičić, Dragana, Slavkovska, Violeta, Mihailov-Krstev, Tatjana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)." in Planta (2019),
https://doi.org/10.1007/s00425-018-03071-5 . .

Uzelac, B.. (2018). Book of Abstracts. in Belgrade : Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology
Belgrade : Serbian Plant Physiology Society; University, Institute for Biological Research "Siniša Stanković"; University,Faculty of Biology., 1-188.
https://hdl.handle.net/21.15107/rcub_ibiss_3162

Uzelac B. Book of Abstracts. in Belgrade : Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology. 2018;:1-188.
https://hdl.handle.net/21.15107/rcub_ibiss_3162 .

Uzelac, Branka, "Book of Abstracts" in Belgrade : Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology (2018):1-188,
https://hdl.handle.net/21.15107/rcub_ibiss_3162 .

TY  - CHAP
AU  - Stojičić, Dragana
AU  - Uzelac, Branka
AU  - Budimir, Snežana
PY  - 2018
UR  - http://link.springer.com/10.1007/978-3-319-89483-6_4
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3270
AB  - Bosnian pine (Pinus heldreichii Christ.) is a Tertiary relict subendemic to the mountains of Balkan Peninsula and a small area in southern Italy.
PB  - Springer
T2  - Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants
T1  - Bosnian Pine Pinus heldreichii Christ.
DO  - 10.1007/978-3-319-89483-6_4
SP  - 49
EP  - 62
ER  - 

@inbook{
author = "Stojičić, Dragana and Uzelac, Branka and Budimir, Snežana",
year = "2018",
abstract = "Bosnian pine (Pinus heldreichii Christ.) is a Tertiary relict subendemic to the mountains of Balkan Peninsula and a small area in southern Italy.",
publisher = "Springer",
journal = "Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants",
booktitle = "Bosnian Pine Pinus heldreichii Christ.",
doi = "10.1007/978-3-319-89483-6_4",
pages = "49-62"
}

Stojičić, D., Uzelac, B.,& Budimir, S.. (2018). Bosnian Pine Pinus heldreichii Christ.. in Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants
Springer., 49-62.
https://doi.org/10.1007/978-3-319-89483-6_4

Stojičić D, Uzelac B, Budimir S. Bosnian Pine Pinus heldreichii Christ.. in Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. 2018;:49-62.
doi:10.1007/978-3-319-89483-6_4 .

Stojičić, Dragana, Uzelac, Branka, Budimir, Snežana, "Bosnian Pine Pinus heldreichii Christ." in Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants (2018):49-62,
https://doi.org/10.1007/978-3-319-89483-6_4 . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Petrić, Marija
AU  - Uzelac, Branka
AU  - Milošević, Snežana
AU  - Vujčić, Zoran
AU  - Stevanović, Jasmina
AU  - Tadić, Jovan
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0304423817307483
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2956
AB  - We investigated the removal of phenol from water solutions (200 mg L−1) using two varieties of lettuce (Lactuca sativa L.) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L−1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L−1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L−1, but were not able to remove phenol at constant concentration above 25 mg L−1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.
T2  - Scientia Horticulturae
T1  - Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1
VL  - 231
DO  - 10.1016/j.scienta.2017.12.025
SP  - 210
EP  - 218
ER  - 

@article{
author = "Tadić, Vojin and Petrić, Marija and Uzelac, Branka and Milošević, Snežana and Vujčić, Zoran and Stevanović, Jasmina and Tadić, Jovan",
year = "2018",
abstract = "We investigated the removal of phenol from water solutions (200 mg L−1) using two varieties of lettuce (Lactuca sativa L.) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L−1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L−1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L−1, but were not able to remove phenol at constant concentration above 25 mg L−1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.",
journal = "Scientia Horticulturae",
title = "Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1",
volume = "231",
doi = "10.1016/j.scienta.2017.12.025",
pages = "210-218"
}

Tadić, V., Petrić, M., Uzelac, B., Milošević, S., Vujčić, Z., Stevanović, J.,& Tadić, J.. (2018). Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1. in Scientia Horticulturae, 231, 210-218.
https://doi.org/10.1016/j.scienta.2017.12.025

Tadić V, Petrić M, Uzelac B, Milošević S, Vujčić Z, Stevanović J, Tadić J. Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1. in Scientia Horticulturae. 2018;231:210-218.
doi:10.1016/j.scienta.2017.12.025 .

Tadić, Vojin, Petrić, Marija, Uzelac, Branka, Milošević, Snežana, Vujčić, Zoran, Stevanović, Jasmina, Tadić, Jovan, "Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1" in Scientia Horticulturae, 231 (2018):210-218,
https://doi.org/10.1016/j.scienta.2017.12.025 . .

TY  - CHAP
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Budimir, Snežana
PY  - 2018
UR  - http://link.springer.com/10.1007/978-1-4939-7672-0_21
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3000
AB  - Leaf senescence constitutes a highly regulated final phase of leaf development, leading to cell death that is recognized as a type of programmed cell death (PCD). Degradation of nuclear DNA into oligonucleosomal fragments (DNA ladder) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay are methods commonly used to detect PCD-specific DNA cleavage. TUNEL reaction in situ labels free 3′-OH DNA strand breaks (nicks), thus allowing histological localization of nuclear DNA degradation during PCD. Here we describe in situ labeling of PCD-specific nuclear DNA fragmentation on conventional histological sections of senescing tobacco leaves. Incorporation of fluorescein-labeled dUTPs is detected by fluorescence microscopy, which enables in situ visualization of PCD at the single-cell level in the leaf mesophyll tissues undergoing senescence.
PB  - Humana Press, New York, NY
T2  - Plant Senescence: Methods and Protocols
T2  - Plant Senescence: Methods and Protocols
T1  - In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay
DO  - 10.1007/978-1-4939-7672-0_21
SP  - 267
EP  - 282
ER  - 

@inbook{
author = "Uzelac, Branka and Janošević, Dušica and Budimir, Snežana",
year = "2018",
abstract = "Leaf senescence constitutes a highly regulated final phase of leaf development, leading to cell death that is recognized as a type of programmed cell death (PCD). Degradation of nuclear DNA into oligonucleosomal fragments (DNA ladder) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay are methods commonly used to detect PCD-specific DNA cleavage. TUNEL reaction in situ labels free 3′-OH DNA strand breaks (nicks), thus allowing histological localization of nuclear DNA degradation during PCD. Here we describe in situ labeling of PCD-specific nuclear DNA fragmentation on conventional histological sections of senescing tobacco leaves. Incorporation of fluorescein-labeled dUTPs is detected by fluorescence microscopy, which enables in situ visualization of PCD at the single-cell level in the leaf mesophyll tissues undergoing senescence.",
publisher = "Humana Press, New York, NY",
journal = "Plant Senescence: Methods and Protocols, Plant Senescence: Methods and Protocols",
booktitle = "In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay",
doi = "10.1007/978-1-4939-7672-0_21",
pages = "267-282"
}

Uzelac, B., Janošević, D.,& Budimir, S.. (2018). In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay. in Plant Senescence: Methods and Protocols
Humana Press, New York, NY., 267-282.
https://doi.org/10.1007/978-1-4939-7672-0_21

Uzelac B, Janošević D, Budimir S. In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay. in Plant Senescence: Methods and Protocols. 2018;:267-282.
doi:10.1007/978-1-4939-7672-0_21 .

Uzelac, Branka, Janošević, Dušica, Budimir, Snežana, "In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay" in Plant Senescence: Methods and Protocols (2018):267-282,
https://doi.org/10.1007/978-1-4939-7672-0_21 . .

TY  - CONF
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6735
AB  - Morphology and structure of Micromeria croatica glandular trichomes in relation to the secretory process were characterized using scanning electron microscopy (SEM) and light microscopy, and their secretion was histochemically analyzed. SEM and histochemical analysis revealed that leaf indumentum of in vitro grown M. croatica consisted of non-glandular and glandular trichomes (Figure 1a). The main types of glandular trichomes observed in M. croatica leaves were peltate and capitate (Figure 1b-d). Peltate trichomes consisted of a broad basal cell embedded in the epidermis, a single-celled stalk, and a large round multicellular head (Figure 1e, h). Two types of capitate trichomes, differing in size and structure, could be distinguished. Type I capitate trichomes were positioned at an angle to the leaf surface and composed of one basal epidermal cell, short cutinized stalk and unicellular ellipsoidal head (Figure 1f). Type II capitate trichomes comprised of one basal cell, unicellular stalk and one apical secretory cell (Figure 1g). Apical cell of mature type II capitate trichomes had well developed round subcuticular space (Figure 1d). Plant extracts secreted from glandular trichomes are a valuable source of biologically active compounds. Phytochemical analysis identified up to 37 compounds in the essential oils (EOs) of wild-growing and micropropagated plants, and revealed differences in both content and the composition of the EOs obtained from different samples. EOs from both native plant material and in vitro cultivated plants showed very high percentages of total monoterpenoids, dominated by oxygenated monoterpenes. 
Borneol was the main essential oil component detected in wild-growing plants (25.28%) and micropropagated plants grown on plant growth regulator-free medium (20.30%). Micropropagated plants cultivated in the presence of 0.3 µM kinetin had the highest yield of the EO, with geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%) being the main EO components. The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. Sesquiterpene hydrocarbons accounted for the majority of detected sesquiterpenoids and were dominated by alpha-cadinene and beta-vetivenene. Some of the volatiles were found only in plant material obtained by micropropagation but not in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties.
PB  - Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society
C3  - Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)
SP  - 302
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6735
ER  - 

@conference{
author = "Tošić, Svetlana and Stojičić, Dragana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2017",
abstract = "Morphology and structure of Micromeria croatica glandular trichomes in relation to the secretory process were characterized using scanning electron microscopy (SEM) and light microscopy, and their secretion was histochemically analyzed. SEM and histochemical analysis revealed that leaf indumentum of in vitro grown M. croatica consisted of non-glandular and glandular trichomes (Figure 1a). The main types of glandular trichomes observed in M. croatica leaves were peltate and capitate (Figure 1b-d). Peltate trichomes consisted of a broad basal cell embedded in the epidermis, a single-celled stalk, and a large round multicellular head (Figure 1e, h). Two types of capitate trichomes, differing in size and structure, could be distinguished. Type I capitate trichomes were positioned at an angle to the leaf surface and composed of one basal epidermal cell, short cutinized stalk and unicellular ellipsoidal head (Figure 1f). Type II capitate trichomes comprised of one basal cell, unicellular stalk and one apical secretory cell (Figure 1g). Apical cell of mature type II capitate trichomes had well developed round subcuticular space (Figure 1d). Plant extracts secreted from glandular trichomes are a valuable source of biologically active compounds. Phytochemical analysis identified up to 37 compounds in the essential oils (EOs) of wild-growing and micropropagated plants, and revealed differences in both content and the composition of the EOs obtained from different samples. EOs from both native plant material and in vitro cultivated plants showed very high percentages of total monoterpenoids, dominated by oxygenated monoterpenes. 
Borneol was the main essential oil component detected in wild-growing plants (25.28%) and micropropagated plants grown on plant growth regulator-free medium (20.30%). Micropropagated plants cultivated in the presence of 0.3 µM kinetin had the highest yield of the EO, with geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%) being the main EO components. The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. Sesquiterpene hydrocarbons accounted for the majority of detected sesquiterpenoids and were dominated by alpha-cadinene and beta-vetivenene. Some of the volatiles were found only in plant material obtained by micropropagation but not in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties.",
publisher = "Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society",
journal = "Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)",
pages = "302-303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6735"
}

Tošić, S., Stojičić, D., Slavkovska, V., Zlatković, B., Budimir, S.,& Uzelac, B.. (2017). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia
Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society., 302-303.
https://hdl.handle.net/21.15107/rcub_ibiss_6735

Tošić S, Stojičić D, Slavkovska V, Zlatković B, Budimir S, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia. 2017;:302-303.
https://hdl.handle.net/21.15107/rcub_ibiss_6735 .

Tošić, Svetlana, Stojičić, Dragana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)" in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia (2017):302-303,
https://hdl.handle.net/21.15107/rcub_ibiss_6735 .