TY  - JOUR
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6553
AB  - Introduction: In the present study, we assessed the effects of the hyper- harmonized- 
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
 Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects 
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis 
through machine learning (ML) using artificial neural networks (ANNs) to classify the 
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment 
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in 
cortical and hippocampal tissue were assessed.
 Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in 
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not 
induce the activation of glia (astrocytes and microglia) nor did it negatively affect 
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
 Conclusion: The obtained results point to the potential of 3HFWC, when applied in 
the presymptomatic phase of AD, to interfere with amyloid plaque formation without 
inducing AD- related pathological processes such as neuroinflammation, gliosis, and 
synaptic vulnerability.
PB  - Wiley
T2  - CNS Neuroscience and Therapeutics
T1  - The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease
IS  - 3
VL  - 30
DO  - 10.1111/cns.14188
SP  - e14188
ER  - 

@article{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2024",
abstract = "Introduction: In the present study, we assessed the effects of the hyper- harmonized- 
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
 Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects 
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis 
through machine learning (ML) using artificial neural networks (ANNs) to classify the 
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment 
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in 
cortical and hippocampal tissue were assessed.
 Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in 
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not 
induce the activation of glia (astrocytes and microglia) nor did it negatively affect 
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
 Conclusion: The obtained results point to the potential of 3HFWC, when applied in 
the presymptomatic phase of AD, to interfere with amyloid plaque formation without 
inducing AD- related pathological processes such as neuroinflammation, gliosis, and 
synaptic vulnerability.",
publisher = "Wiley",
journal = "CNS Neuroscience and Therapeutics",
title = "The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease",
number = "3",
volume = "30",
doi = "10.1111/cns.14188",
pages = "e14188"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2024). The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease. in CNS Neuroscience and Therapeutics
Wiley., 30(3), e14188.
https://doi.org/10.1111/cns.14188

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease. in CNS Neuroscience and Therapeutics. 2024;30(3):e14188.
doi:10.1111/cns.14188 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease" in CNS Neuroscience and Therapeutics, 30, no. 3 (2024):e14188,
https://doi.org/10.1111/cns.14188 . .

TY  - JOUR
AU  - Jovičić, Katarina
AU  - Đikanović, Vesna
AU  - Santrač, Isidora
AU  - Živković, Sanja
AU  - Dimitrijević, Milena
AU  - Vranković, Jelena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6636
AB  - In this study, the concentrations of metals and trace elements (As, Cd, Co, Cr, Cu, Hg, Ni,
Pb and Zn) were determined in the muscle tissue of adult roach and white bream at two different
sites in the Belgrade section of the Danube. Twenty-six fatty acids, consisting of nine saturated FA
(SFAs), seven monosaturated FA (MUFAs) and ten polysaturated FA (PUFAs), were identified. The
analysis of the concentration of metals and trace elements of the roach and white bream showed
species-specific differences in their bioaccumulation. Four of all elements analyzed (As, Hg, Ni and
Pb) correlated significantly with the changes in FA profiles in fish from both sampling sites, with the
exception of Cu, which correlated with the FA profile at the site before, and Zn, whose concentration
influenced the FA profile at the site after wastewater discharges. The lower PUFA content in the fish
from a site under higher environment pressure could indicate that the fish are stressed. The results
suggest that changes in lipid composition may be one of the protective mechanisms of cells to cope
with anthropogenic stressors.
PB  - Basel: MDPI
T2  - Animals
T1  - Effects of Trace Elements on the Fatty Acid Composition in Danubian Fish Species
IS  - 954
VL  - 14
DO  - 10.3390/ani14060954
SP  - 954
ER  - 

@article{
author = "Jovičić, Katarina and Đikanović, Vesna and Santrač, Isidora and Živković, Sanja and Dimitrijević, Milena and Vranković, Jelena",
year = "2024",
abstract = "In this study, the concentrations of metals and trace elements (As, Cd, Co, Cr, Cu, Hg, Ni,
Pb and Zn) were determined in the muscle tissue of adult roach and white bream at two different
sites in the Belgrade section of the Danube. Twenty-six fatty acids, consisting of nine saturated FA
(SFAs), seven monosaturated FA (MUFAs) and ten polysaturated FA (PUFAs), were identified. The
analysis of the concentration of metals and trace elements of the roach and white bream showed
species-specific differences in their bioaccumulation. Four of all elements analyzed (As, Hg, Ni and
Pb) correlated significantly with the changes in FA profiles in fish from both sampling sites, with the
exception of Cu, which correlated with the FA profile at the site before, and Zn, whose concentration
influenced the FA profile at the site after wastewater discharges. The lower PUFA content in the fish
from a site under higher environment pressure could indicate that the fish are stressed. The results
suggest that changes in lipid composition may be one of the protective mechanisms of cells to cope
with anthropogenic stressors.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "Effects of Trace Elements on the Fatty Acid Composition in Danubian Fish Species",
number = "954",
volume = "14",
doi = "10.3390/ani14060954",
pages = "954"
}

Jovičić, K., Đikanović, V., Santrač, I., Živković, S., Dimitrijević, M.,& Vranković, J.. (2024). Effects of Trace Elements on the Fatty Acid Composition in Danubian Fish Species. in Animals
Basel: MDPI., 14(954), 954.
https://doi.org/10.3390/ani14060954

Jovičić K, Đikanović V, Santrač I, Živković S, Dimitrijević M, Vranković J. Effects of Trace Elements on the Fatty Acid Composition in Danubian Fish Species. in Animals. 2024;14(954):954.
doi:10.3390/ani14060954 .

Jovičić, Katarina, Đikanović, Vesna, Santrač, Isidora, Živković, Sanja, Dimitrijević, Milena, Vranković, Jelena, "Effects of Trace Elements on the Fatty Acid Composition in Danubian Fish Species" in Animals, 14, no. 954 (2024):954,
https://doi.org/10.3390/ani14060954 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Pavlović, Slađan
AU  - Dinčić, Evica
AU  - Kuveljić, Jovana
AU  - Manojlović, Dragan
AU  - Živković, Maja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6584
AB  - Background: Multiple sclerosis (MS) is a chronic demyelinating disorder intricately linked to perturbations in
trace element levels. While previous studies have explored circulating trace elements in a limited sample, understanding the impact of demographic and clinical variables on the elemental profile within a larger cohort
remains elusive.
Methods: This study aimed to evaluate essential trace elements (Cr, Mn, Co, Cu, Zn, and Se) in the sera of 215 MS
patients compared to a meticulously matched control group of 100 individuals with similar gender and age. Our
main objective was to identify potential variations in elemental profiles based on demographic and clinical
parameters among MS patients, elucidating the prospective relevance of supplementing specific essential trace
elements.
Results: Data indicated a significant decrease in serum levels of Mn, Co, Zn, and Se, and an increase in Cr in MS
patients compared to controls. These trace elements not only discriminated between MS patients and controls but
also exhibited distinctive capabilities among demographic subgroups. Gender, smoking habits, and age strata
(20–40 years and 41–60 years) revealed discernible variations in elemental profiles between MS patients and
their control counterparts. Se demonstrated the singular ability to stratify cases of extreme MS severity, mild
relapsing-remitting MS (RRMS) and highly severe secondary progressive MS (SPMS). In contrast, Co significantly
differentiated RRMS from primary progressive MS (PPMS), while Cu significantly differentiated SPMS from
PPMS. Additionally, Cu showed a negative correlation with MSSS, while Mn and Zn showed a positive correlation
with EDSS.
Conclusion: These findings underscore a substantive deficiency in Mn, Co, Zn, and Se in the MS cohort, supporting
targeted supplementation with these trace elements. This study provides a comprehensive understanding of the
intricate relationship between essential trace elements and MS, paving the way for further research into
personalized nutritional interventions for this complex neurological disorder.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation
VL  - 83
DO  - 10.1016/j.jtemb.2024.127421
SP  - 127421
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Jagodić, Jovana and Pavlović, Slađan and Dinčić, Evica and Kuveljić, Jovana and Manojlović, Dragan and Živković, Maja",
year = "2024",
abstract = "Background: Multiple sclerosis (MS) is a chronic demyelinating disorder intricately linked to perturbations in
trace element levels. While previous studies have explored circulating trace elements in a limited sample, understanding the impact of demographic and clinical variables on the elemental profile within a larger cohort
remains elusive.
Methods: This study aimed to evaluate essential trace elements (Cr, Mn, Co, Cu, Zn, and Se) in the sera of 215 MS
patients compared to a meticulously matched control group of 100 individuals with similar gender and age. Our
main objective was to identify potential variations in elemental profiles based on demographic and clinical
parameters among MS patients, elucidating the prospective relevance of supplementing specific essential trace
elements.
Results: Data indicated a significant decrease in serum levels of Mn, Co, Zn, and Se, and an increase in Cr in MS
patients compared to controls. These trace elements not only discriminated between MS patients and controls but
also exhibited distinctive capabilities among demographic subgroups. Gender, smoking habits, and age strata
(20–40 years and 41–60 years) revealed discernible variations in elemental profiles between MS patients and
their control counterparts. Se demonstrated the singular ability to stratify cases of extreme MS severity, mild
relapsing-remitting MS (RRMS) and highly severe secondary progressive MS (SPMS). In contrast, Co significantly
differentiated RRMS from primary progressive MS (PPMS), while Cu significantly differentiated SPMS from
PPMS. Additionally, Cu showed a negative correlation with MSSS, while Mn and Zn showed a positive correlation
with EDSS.
Conclusion: These findings underscore a substantive deficiency in Mn, Co, Zn, and Se in the MS cohort, supporting
targeted supplementation with these trace elements. This study provides a comprehensive understanding of the
intricate relationship between essential trace elements and MS, paving the way for further research into
personalized nutritional interventions for this complex neurological disorder.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation",
volume = "83",
doi = "10.1016/j.jtemb.2024.127421",
pages = "127421"
}

Stojsavljević, A., Jagodić, J., Pavlović, S., Dinčić, E., Kuveljić, J., Manojlović, D.,& Živković, M.. (2024). Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation. in Journal of Trace Elements in Medicine and Biology
Elsevier., 83, 127421.
https://doi.org/10.1016/j.jtemb.2024.127421

Stojsavljević A, Jagodić J, Pavlović S, Dinčić E, Kuveljić J, Manojlović D, Živković M. Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation. in Journal of Trace Elements in Medicine and Biology. 2024;83:127421.
doi:10.1016/j.jtemb.2024.127421 .

Stojsavljević, Aleksandar, Jagodić, Jovana, Pavlović, Slađan, Dinčić, Evica, Kuveljić, Jovana, Manojlović, Dragan, Živković, Maja, "Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation" in Journal of Trace Elements in Medicine and Biology, 83 (2024):127421,
https://doi.org/10.1016/j.jtemb.2024.127421 . .

TY  - CONF
AU  - Simeunović, Valentina
AU  - Todorović, Smilja
AU  - Prvulović, Milica
AU  - Sokanović, Srđan
AU  - Jović, Milena
AU  - Vukojević, Anđela
AU  - Milanović, Desanka
AU  - Mladenović, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5807
AB  - Dietary restriction (DR) is one of the most studied environmental factors, which has 
been shown to prolong life and enhance health. However, the outcome of DR depends 
on both the duration of DR as well as the age of the individual at the onset of food 
restriction. A few studies have been conducted on the impacts of DR during puberty, 
but mainly casting doubt on beneficial effects it has on the individual. 
In this experiment we studied the effect of 30% DR (70% of ad libitum daily intake) 
during puberty on locomotor and vertical activity of 6- and 18-month-old female 
Wistar rats. We examined the effects of DR of different onsets and durations: first one 
that lasted from 28th to 35th day of life (Early Diet, ED), second lasted from 35th to 
42nd day of life (Late Diet, LD), and the one that lasted from 28th to 42nd day of life 
(Combined Diet, CD). The Open Field Test was used for tracking locomotor and 
vertical activity.
Our findings demonstrated that in 6-month-old females all three types of DR 
increased locomotor and vertical movement. Although the impact of DR during 
puberty is evident in adults, it is lost during aging, as older animals showed no 
significant change in locomotor or vertical activity under any type of DR.
PB  - Belgrade : Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Dietary restriction during puberty changes locomotor and vertical  activity of adult female Wistar rats in an onset- and duration-dependent manner
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5807
ER  - 

@conference{
author = "Simeunović, Valentina and Todorović, Smilja and Prvulović, Milica and Sokanović, Srđan and Jović, Milena and Vukojević, Anđela and Milanović, Desanka and Mladenović, Aleksandra",
year = "2023",
abstract = "Dietary restriction (DR) is one of the most studied environmental factors, which has 
been shown to prolong life and enhance health. However, the outcome of DR depends 
on both the duration of DR as well as the age of the individual at the onset of food 
restriction. A few studies have been conducted on the impacts of DR during puberty, 
but mainly casting doubt on beneficial effects it has on the individual. 
In this experiment we studied the effect of 30% DR (70% of ad libitum daily intake) 
during puberty on locomotor and vertical activity of 6- and 18-month-old female 
Wistar rats. We examined the effects of DR of different onsets and durations: first one 
that lasted from 28th to 35th day of life (Early Diet, ED), second lasted from 35th to 
42nd day of life (Late Diet, LD), and the one that lasted from 28th to 42nd day of life 
(Combined Diet, CD). The Open Field Test was used for tracking locomotor and 
vertical activity.
Our findings demonstrated that in 6-month-old females all three types of DR 
increased locomotor and vertical movement. Although the impact of DR during 
puberty is evident in adults, it is lost during aging, as older animals showed no 
significant change in locomotor or vertical activity under any type of DR.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Dietary restriction during puberty changes locomotor and vertical  activity of adult female Wistar rats in an onset- and duration-dependent manner",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5807"
}

Simeunović, V., Todorović, S., Prvulović, M., Sokanović, S., Jović, M., Vukojević, A., Milanović, D.,& Mladenović, A.. (2023). Dietary restriction during puberty changes locomotor and vertical  activity of adult female Wistar rats in an onset- and duration-dependent manner. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade : Serbian Neuroscience Society., 69.
https://hdl.handle.net/21.15107/rcub_ibiss_5807

Simeunović V, Todorović S, Prvulović M, Sokanović S, Jović M, Vukojević A, Milanović D, Mladenović A. Dietary restriction during puberty changes locomotor and vertical  activity of adult female Wistar rats in an onset- and duration-dependent manner. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:69.
https://hdl.handle.net/21.15107/rcub_ibiss_5807 .

Simeunović, Valentina, Todorović, Smilja, Prvulović, Milica, Sokanović, Srđan, Jović, Milena, Vukojević, Anđela, Milanović, Desanka, Mladenović, Aleksandra, "Dietary restriction during puberty changes locomotor and vertical  activity of adult female Wistar rats in an onset- and duration-dependent manner" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):69,
https://hdl.handle.net/21.15107/rcub_ibiss_5807 .

TY  - JOUR
AU  - Božinović, Nevena
AU  - Savva, Kyriaki
AU  - Rajić, Vladimir
AU  - Popović, Maja
AU  - Tošić, Dragana
AU  - Janjetović, Kristina
AU  - Despotović, Ana
AU  - Zogović, Nevena
AU  - Stratakis, Emmanuel
AU  - Petrović, Suzana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6271
AB  - The creation of novel biocompatible Ti-based thin films with a Zr or Cu sub-layer modified by ultrafast laser processing is studied. To prepare bioactive surfaces, ultrafast laser processing is focused on the formation of laser-induced periodic surface structures (LIPSS) with the production of oxide phases at the surfaces. Two differently designed multilayer thin films Ti/Cu/Ti and Ti/Zr/Ti were deposited on the silicon using the ion sputtering method. The Ti thin film contains Cu or Zr sub-layer (thickness of 10 nm) at the 10 nm below the surface. The composition and surface morphology variations for these systems, deposited and laser-processed under the same experimental conditions, were caused only by different thermo-physical properties of the sub-layer (Cu or Zr). The surface morphology in the form of LIPSS, led to improved cell adhesion and stable cells/thin films interface compared to as-deposited samples. Field-emission scanning electron microscopy and MTT analysis revealed that laser processing of both systems increased cell adhesion, proliferation, and metabolical activity of L929 mouse fibroblast cells compared to non-modified flat surfaces. Overall, the biocompatibility of Zr-containing thin films is better than Ti/Cu/Ti system. Further, laser processing and formation of LIPSS makes Ti/Zr/Ti thin films excellent candidate for biomedical.
PB  - Amsterdam: Elsevier
T2  - Materials Chemistry and Physics
T1  - Influence of zirconium and copper sub-layer in cell integrations on femtosecond laser-processed Ti thin films
VL  - 308
DO  - 10.1016/j.matchemphys.2023.128286
SP  - 128286
ER  - 

@article{
author = "Božinović, Nevena and Savva, Kyriaki and Rajić, Vladimir and Popović, Maja and Tošić, Dragana and Janjetović, Kristina and Despotović, Ana and Zogović, Nevena and Stratakis, Emmanuel and Petrović, Suzana",
year = "2023",
abstract = "The creation of novel biocompatible Ti-based thin films with a Zr or Cu sub-layer modified by ultrafast laser processing is studied. To prepare bioactive surfaces, ultrafast laser processing is focused on the formation of laser-induced periodic surface structures (LIPSS) with the production of oxide phases at the surfaces. Two differently designed multilayer thin films Ti/Cu/Ti and Ti/Zr/Ti were deposited on the silicon using the ion sputtering method. The Ti thin film contains Cu or Zr sub-layer (thickness of 10 nm) at the 10 nm below the surface. The composition and surface morphology variations for these systems, deposited and laser-processed under the same experimental conditions, were caused only by different thermo-physical properties of the sub-layer (Cu or Zr). The surface morphology in the form of LIPSS, led to improved cell adhesion and stable cells/thin films interface compared to as-deposited samples. Field-emission scanning electron microscopy and MTT analysis revealed that laser processing of both systems increased cell adhesion, proliferation, and metabolical activity of L929 mouse fibroblast cells compared to non-modified flat surfaces. Overall, the biocompatibility of Zr-containing thin films is better than Ti/Cu/Ti system. Further, laser processing and formation of LIPSS makes Ti/Zr/Ti thin films excellent candidate for biomedical.",
publisher = "Amsterdam: Elsevier",
journal = "Materials Chemistry and Physics",
title = "Influence of zirconium and copper sub-layer in cell integrations on femtosecond laser-processed Ti thin films",
volume = "308",
doi = "10.1016/j.matchemphys.2023.128286",
pages = "128286"
}

Božinović, N., Savva, K., Rajić, V., Popović, M., Tošić, D., Janjetović, K., Despotović, A., Zogović, N., Stratakis, E.,& Petrović, S.. (2023). Influence of zirconium and copper sub-layer in cell integrations on femtosecond laser-processed Ti thin films. in Materials Chemistry and Physics
Amsterdam: Elsevier., 308, 128286.
https://doi.org/10.1016/j.matchemphys.2023.128286

Božinović N, Savva K, Rajić V, Popović M, Tošić D, Janjetović K, Despotović A, Zogović N, Stratakis E, Petrović S. Influence of zirconium and copper sub-layer in cell integrations on femtosecond laser-processed Ti thin films. in Materials Chemistry and Physics. 2023;308:128286.
doi:10.1016/j.matchemphys.2023.128286 .

Božinović, Nevena, Savva, Kyriaki, Rajić, Vladimir, Popović, Maja, Tošić, Dragana, Janjetović, Kristina, Despotović, Ana, Zogović, Nevena, Stratakis, Emmanuel, Petrović, Suzana, "Influence of zirconium and copper sub-layer in cell integrations on femtosecond laser-processed Ti thin films" in Materials Chemistry and Physics, 308 (2023):128286,
https://doi.org/10.1016/j.matchemphys.2023.128286 . .

TY  - JOUR
AU  - Grigorov, Ilijana
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Veljković, Filip
AU  - Miletić-Vukajlović, Jadranka
AU  - Bobić, Katarina
AU  - Soldatović, Ivan
AU  - Đurašević, Siniša
AU  - Jasnić, Nebojša
AU  - Stanković, Sanja
AU  - Glumac, Sofija
AU  - Mihailović-Vučinić, Violeta
AU  - Milenković, Branislava
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6126
AB  - The careful monitoring of patients with mild/moderate COVID-19 is of particular importance because of the rapid progression of complications associated with COVID-19. For prognostic reasons and for the economic management of health care resources, additional biomarkers need to be identified, and their monitoring can conceivably be performed in the early stages of the disease. In this retrospective cross-sectional study, we found that serum concentrations of high-mobility group box 1 (HMGB1) and heme oxygenase-1 (HO-1), at the time of hospital admission, could be useful biomarkers for COVID-19 management. The study included 160 randomly selected recovered patients with mild to moderate COVID-19 on admission. Compared with healthy controls, serum HMGB1 and HO-1 levels increased by 487.6 pg/mL versus 43.1 pg/mL and 1497.7 pg/mL versus 756.1 pg/mL, respectively. Serum HO-1 correlated significantly with serum HMGB1, oxidative stress parameters (malondialdehyde (MDA), the phosphatidylcholine/lysophosphatidylcholine ratio (PC/LPC), the ratio of reduced and oxidative glutathione (GSH/GSSG)), and anti-inflammatory acute phase proteins (ferritin, haptoglobin). Increased heme catabolism/hemolysis were not detected. We hypothesize that the increase in HO-1 in the early phase of COVID-19 disease is likely to have a survival benefit by providing protection against oxidative stress and inflammation, whereas the level of HMGB1 increase reflects the activity of the innate immune system and represents levels within which the disease can be kept under control.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Serum High-Mobility Group Box 1 and Heme Oxygenase 1 as Biomarkers in COVID-19 Patients at Hospital Admission
IS  - 17
VL  - 24
DO  - 10.3390/ijms241713164
SP  - 13164
ER  - 

@article{
author = "Grigorov, Ilijana and Pejić, Snežana and Todorović, Ana and Drakulić, Dunja and Veljković, Filip and Miletić-Vukajlović, Jadranka and Bobić, Katarina and Soldatović, Ivan and Đurašević, Siniša and Jasnić, Nebojša and Stanković, Sanja and Glumac, Sofija and Mihailović-Vučinić, Violeta and Milenković, Branislava",
year = "2023",
abstract = "The careful monitoring of patients with mild/moderate COVID-19 is of particular importance because of the rapid progression of complications associated with COVID-19. For prognostic reasons and for the economic management of health care resources, additional biomarkers need to be identified, and their monitoring can conceivably be performed in the early stages of the disease. In this retrospective cross-sectional study, we found that serum concentrations of high-mobility group box 1 (HMGB1) and heme oxygenase-1 (HO-1), at the time of hospital admission, could be useful biomarkers for COVID-19 management. The study included 160 randomly selected recovered patients with mild to moderate COVID-19 on admission. Compared with healthy controls, serum HMGB1 and HO-1 levels increased by 487.6 pg/mL versus 43.1 pg/mL and 1497.7 pg/mL versus 756.1 pg/mL, respectively. Serum HO-1 correlated significantly with serum HMGB1, oxidative stress parameters (malondialdehyde (MDA), the phosphatidylcholine/lysophosphatidylcholine ratio (PC/LPC), the ratio of reduced and oxidative glutathione (GSH/GSSG)), and anti-inflammatory acute phase proteins (ferritin, haptoglobin). Increased heme catabolism/hemolysis were not detected. We hypothesize that the increase in HO-1 in the early phase of COVID-19 disease is likely to have a survival benefit by providing protection against oxidative stress and inflammation, whereas the level of HMGB1 increase reflects the activity of the innate immune system and represents levels within which the disease can be kept under control.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Serum High-Mobility Group Box 1 and Heme Oxygenase 1 as Biomarkers in COVID-19 Patients at Hospital Admission",
number = "17",
volume = "24",
doi = "10.3390/ijms241713164",
pages = "13164"
}

Grigorov, I., Pejić, S., Todorović, A., Drakulić, D., Veljković, F., Miletić-Vukajlović, J., Bobić, K., Soldatović, I., Đurašević, S., Jasnić, N., Stanković, S., Glumac, S., Mihailović-Vučinić, V.,& Milenković, B.. (2023). Serum High-Mobility Group Box 1 and Heme Oxygenase 1 as Biomarkers in COVID-19 Patients at Hospital Admission. in International Journal of Molecular Sciences
Basel: MDPI., 24(17), 13164.
https://doi.org/10.3390/ijms241713164

Grigorov I, Pejić S, Todorović A, Drakulić D, Veljković F, Miletić-Vukajlović J, Bobić K, Soldatović I, Đurašević S, Jasnić N, Stanković S, Glumac S, Mihailović-Vučinić V, Milenković B. Serum High-Mobility Group Box 1 and Heme Oxygenase 1 as Biomarkers in COVID-19 Patients at Hospital Admission. in International Journal of Molecular Sciences. 2023;24(17):13164.
doi:10.3390/ijms241713164 .

Grigorov, Ilijana, Pejić, Snežana, Todorović, Ana, Drakulić, Dunja, Veljković, Filip, Miletić-Vukajlović, Jadranka, Bobić, Katarina, Soldatović, Ivan, Đurašević, Siniša, Jasnić, Nebojša, Stanković, Sanja, Glumac, Sofija, Mihailović-Vučinić, Violeta, Milenković, Branislava, "Serum High-Mobility Group Box 1 and Heme Oxygenase 1 as Biomarkers in COVID-19 Patients at Hospital Admission" in International Journal of Molecular Sciences, 24, no. 17 (2023):13164,
https://doi.org/10.3390/ijms241713164 . .

TY  - CONF
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Sobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5858
AB  - Mfsd2a is expressed mainly in the endothelial cells and is an essential regulator of 
blood vessel transcytosis. Therefore, decrease in Mfsd2a expression can be a risk 
factor for developing leaky blood vessels. Mfsd2a is also the main docosahexaenoic 
acid (DHA, C22:6n3) transporter. DHA, an omega-3 fatty acid, is one of the main 
structural lipids of the neuronal and vascular retina, crucial for the normal functioning 
of photoreceptors (PRs). However, the capacity of the retina to synthesize DHA is 
limited, and the maintenance of retinal DHA content relies on the uptake from blood borne lipids. The currently recommended FO doses yielded low PUFAs tissue 
bioavailability, and supplementation with higher doses has been increasingly 
recommended. Nevertheless, the effects of higher FO doses on retinal Mfsd2a 
expression and blood vessels coverage are unknown.
Western blot and qPCR analyses showed that high dose FO supplementation increased 
Mfsd2a expression in the retina. Immunohistochemical analyses of Mfsd2a expression 
on retinal blood vessels (labeled with 488-conjugated Lycopersicon esculentum, 
lectin) and subsequent ImageJ analyses revealed 1.32-fold increase in the Mfsd2a 
retinal blood vessel coverage. In the same time the pericyte blood vessel coverage 
(CD13+ cells) was not affected with FO supplementation, and the increase in Mfsd2a 
blood vessel expression is not the result of the increased pericyte coverage. 
Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of 
the retinal blood vessels that can serve either as prophylaxis in the healthy eye or as an 
adjuvant in developing targeted manipulations of the barrier during diseases.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5858
ER  - 

@conference{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Sobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Mfsd2a is expressed mainly in the endothelial cells and is an essential regulator of 
blood vessel transcytosis. Therefore, decrease in Mfsd2a expression can be a risk 
factor for developing leaky blood vessels. Mfsd2a is also the main docosahexaenoic 
acid (DHA, C22:6n3) transporter. DHA, an omega-3 fatty acid, is one of the main 
structural lipids of the neuronal and vascular retina, crucial for the normal functioning 
of photoreceptors (PRs). However, the capacity of the retina to synthesize DHA is 
limited, and the maintenance of retinal DHA content relies on the uptake from blood borne lipids. The currently recommended FO doses yielded low PUFAs tissue 
bioavailability, and supplementation with higher doses has been increasingly 
recommended. Nevertheless, the effects of higher FO doses on retinal Mfsd2a 
expression and blood vessels coverage are unknown.
Western blot and qPCR analyses showed that high dose FO supplementation increased 
Mfsd2a expression in the retina. Immunohistochemical analyses of Mfsd2a expression 
on retinal blood vessels (labeled with 488-conjugated Lycopersicon esculentum, 
lectin) and subsequent ImageJ analyses revealed 1.32-fold increase in the Mfsd2a 
retinal blood vessel coverage. In the same time the pericyte blood vessel coverage 
(CD13+ cells) was not affected with FO supplementation, and the increase in Mfsd2a 
blood vessel expression is not the result of the increased pericyte coverage. 
Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of 
the retinal blood vessels that can serve either as prophylaxis in the healthy eye or as an 
adjuvant in developing targeted manipulations of the barrier during diseases.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice",
pages = "66",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5858"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Sobajić, S., Kanazir, S.,& Ivković, S.. (2023). The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 66.
https://hdl.handle.net/21.15107/rcub_ibiss_5858

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Sobajić S, Kanazir S, Ivković S. The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:66.
https://hdl.handle.net/21.15107/rcub_ibiss_5858 .

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Sobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):66,
https://hdl.handle.net/21.15107/rcub_ibiss_5858 .

TY  - CONF
AU  - Jović, Milena
AU  - Simeunović, Valentina
AU  - Vukojević, Anđela
AU  - Prvulović, Milica
AU  - Sokanović, Srđan
AU  - Mladenović, Aleksandra
AU  - Milanović, Desanka
AU  - Todorović, Smilja
AU  - Lončarević-Vasiljković, Nataša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5851
AB  - According to the amyloid hypothesis of Alcheimer’s disease (AD), disruption of 
balance between production and clearance of Aβ from the cells leads to the 
progressive accumulation and aggregation of amyloid beta (Aβ) peptides in the brain. 
The glial system (microglial cells and astrocytes) is responsible for maintaining 
homeostasis in the brain which implies its important role in the development and 
progression of AD. Our previous work revealed that the short-term fish oil (FO) 
treatment in 5xFAD mice, (AD animal model), reduces toxic Aβ load and increases 
number of microglial/macrophage cells in parietal cortex. In the present study we 
aimed to further decipher the roles of microglial and macrophage cells and to 
elucidate possible mechanisms responsible for observed reduced level of toxic Aβ42 
peptide. For this purpose, western blot and immunohistochemical analysis were used 
to detect changes in parietal cortex of three-month-old 5xFAD mice after three weeks 
FO treatment (100μl/animal/day). Distinction between microglial cells and 
macrophages was assessed using double immunostaining with anti-TMEM119 and 
anti-Iba1 antibodies respectively. Immunostaining was observed by confocal 
microscopy. For western blot analysis anti-TREM-2 and anti-IDE were used to 
observe potential mechanism responsible for extracellular clearance of toxic Aβ 
forms. Quantification was done by Image Quant software. Our results showed that 
short-term FO supplementation affects the localization and number of microglial cells 
and macrophages. Macrophages were located around the plaque and were responsible 
for the formation of a mechanical barrier, while microglial cells showed an increased 
number under the treatment and were located far from the plaques. Furthermore, the 
treatment did not seem to affect the level of IDE, while on the other hand it 
significantly increased the level of TREM-2 (ultimately sustaining the microglial 
response to Aβ-plaque-induced pathology).
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Short-term fish oil treatment increases number of microglial cells  and expression level of TREM-2 in parietal cortex of 5XFAD mice
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5851
ER  - 

@conference{
author = "Jović, Milena and Simeunović, Valentina and Vukojević, Anđela and Prvulović, Milica and Sokanović, Srđan and Mladenović, Aleksandra and Milanović, Desanka and Todorović, Smilja and Lončarević-Vasiljković, Nataša",
year = "2023",
abstract = "According to the amyloid hypothesis of Alcheimer’s disease (AD), disruption of 
balance between production and clearance of Aβ from the cells leads to the 
progressive accumulation and aggregation of amyloid beta (Aβ) peptides in the brain. 
The glial system (microglial cells and astrocytes) is responsible for maintaining 
homeostasis in the brain which implies its important role in the development and 
progression of AD. Our previous work revealed that the short-term fish oil (FO) 
treatment in 5xFAD mice, (AD animal model), reduces toxic Aβ load and increases 
number of microglial/macrophage cells in parietal cortex. In the present study we 
aimed to further decipher the roles of microglial and macrophage cells and to 
elucidate possible mechanisms responsible for observed reduced level of toxic Aβ42 
peptide. For this purpose, western blot and immunohistochemical analysis were used 
to detect changes in parietal cortex of three-month-old 5xFAD mice after three weeks 
FO treatment (100μl/animal/day). Distinction between microglial cells and 
macrophages was assessed using double immunostaining with anti-TMEM119 and 
anti-Iba1 antibodies respectively. Immunostaining was observed by confocal 
microscopy. For western blot analysis anti-TREM-2 and anti-IDE were used to 
observe potential mechanism responsible for extracellular clearance of toxic Aβ 
forms. Quantification was done by Image Quant software. Our results showed that 
short-term FO supplementation affects the localization and number of microglial cells 
and macrophages. Macrophages were located around the plaque and were responsible 
for the formation of a mechanical barrier, while microglial cells showed an increased 
number under the treatment and were located far from the plaques. Furthermore, the 
treatment did not seem to affect the level of IDE, while on the other hand it 
significantly increased the level of TREM-2 (ultimately sustaining the microglial 
response to Aβ-plaque-induced pathology).",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Short-term fish oil treatment increases number of microglial cells  and expression level of TREM-2 in parietal cortex of 5XFAD mice",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5851"
}

Jović, M., Simeunović, V., Vukojević, A., Prvulović, M., Sokanović, S., Mladenović, A., Milanović, D., Todorović, S.,& Lončarević-Vasiljković, N.. (2023). Short-term fish oil treatment increases number of microglial cells  and expression level of TREM-2 in parietal cortex of 5XFAD mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 65.
https://hdl.handle.net/21.15107/rcub_ibiss_5851

Jović M, Simeunović V, Vukojević A, Prvulović M, Sokanović S, Mladenović A, Milanović D, Todorović S, Lončarević-Vasiljković N. Short-term fish oil treatment increases number of microglial cells  and expression level of TREM-2 in parietal cortex of 5XFAD mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:65.
https://hdl.handle.net/21.15107/rcub_ibiss_5851 .

Jović, Milena, Simeunović, Valentina, Vukojević, Anđela, Prvulović, Milica, Sokanović, Srđan, Mladenović, Aleksandra, Milanović, Desanka, Todorović, Smilja, Lončarević-Vasiljković, Nataša, "Short-term fish oil treatment increases number of microglial cells  and expression level of TREM-2 in parietal cortex of 5XFAD mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):65,
https://hdl.handle.net/21.15107/rcub_ibiss_5851 .

TY  - CONF
AU  - Vukojević, Anđela
AU  - Prvulović, Milica
AU  - Todorović, Smilja
AU  - Mladenović, Aleksandra
AU  - Sokanović, Srđan
AU  - Simeunović, Valentina
AU  - Jović, Milena
AU  - Milanović, Desanka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5850
AB  - Systemic inflammation can be triggered by structural components of gut bacteria such 
as LPS, leading to a cascade of inflammatory pathways involving cytokines and other 
pro-inflammatory molecules. Dietary interventions have been shown to influence 
these pathways. Fermented food as a rich source of nutrients, phytochemicals and 
bioactive compounds, have the significant biological activities, such as anti inflammatory and immunomodulatory functions. In addition, some of these functions 
are due to the high content of lactic acid bacteria (LAB) and their products.
The aim of this study was to investigate the effects of sauerkraut brine (SB) on 
lipopolysaccharides (LPS)-induced central and peripheral inflammation in C57BL/6 
mice. Ninety postnatal day-old mice were divided into 3 groups: naive, treated with 
either 150 µl sauerkraut brine and pasteurized sauerkraut brine (PSB) by oral 
administration for 5 weeks. Control animals (CON) received an equivalent amount of 
saline. Both groups received LPS (0.5 mg/kg. i.p.) 3 hours before sacrifice. Brain and 
liver were isolated for PCR and Western blot analyzes.
The SB and PSB treatments did not affect body weight and behavior of mice 
compared with CON mice. At the molecular level, the sauerkraut brine affected the 
TLR4-MyD88 signaling pathway, resulting in a reduction of cytokines and other 
inflammatory molecules in mice cortex, which was not the case in the liver. Since 
analysis of sauerkraut revealed a high abundance of lactic acid bacteria (LAB) 
(1.8x106
/gr), future studies should clarify whether the anti-inflammatory effect of 
sauerkraut brine is mediated by the mouse microbiota.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Effect of sauerkraut brine in central and peripheral LPS-induced  inflammation in C57BL/6 mice
SP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5850
ER  - 

@conference{
author = "Vukojević, Anđela and Prvulović, Milica and Todorović, Smilja and Mladenović, Aleksandra and Sokanović, Srđan and Simeunović, Valentina and Jović, Milena and Milanović, Desanka",
year = "2023",
abstract = "Systemic inflammation can be triggered by structural components of gut bacteria such 
as LPS, leading to a cascade of inflammatory pathways involving cytokines and other 
pro-inflammatory molecules. Dietary interventions have been shown to influence 
these pathways. Fermented food as a rich source of nutrients, phytochemicals and 
bioactive compounds, have the significant biological activities, such as anti inflammatory and immunomodulatory functions. In addition, some of these functions 
are due to the high content of lactic acid bacteria (LAB) and their products.
The aim of this study was to investigate the effects of sauerkraut brine (SB) on 
lipopolysaccharides (LPS)-induced central and peripheral inflammation in C57BL/6 
mice. Ninety postnatal day-old mice were divided into 3 groups: naive, treated with 
either 150 µl sauerkraut brine and pasteurized sauerkraut brine (PSB) by oral 
administration for 5 weeks. Control animals (CON) received an equivalent amount of 
saline. Both groups received LPS (0.5 mg/kg. i.p.) 3 hours before sacrifice. Brain and 
liver were isolated for PCR and Western blot analyzes.
The SB and PSB treatments did not affect body weight and behavior of mice 
compared with CON mice. At the molecular level, the sauerkraut brine affected the 
TLR4-MyD88 signaling pathway, resulting in a reduction of cytokines and other 
inflammatory molecules in mice cortex, which was not the case in the liver. Since 
analysis of sauerkraut revealed a high abundance of lactic acid bacteria (LAB) 
(1.8x106
/gr), future studies should clarify whether the anti-inflammatory effect of 
sauerkraut brine is mediated by the mouse microbiota.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Effect of sauerkraut brine in central and peripheral LPS-induced  inflammation in C57BL/6 mice",
pages = "68",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5850"
}

Vukojević, A., Prvulović, M., Todorović, S., Mladenović, A., Sokanović, S., Simeunović, V., Jović, M.,& Milanović, D.. (2023). Effect of sauerkraut brine in central and peripheral LPS-induced  inflammation in C57BL/6 mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 68.
https://hdl.handle.net/21.15107/rcub_ibiss_5850

Vukojević A, Prvulović M, Todorović S, Mladenović A, Sokanović S, Simeunović V, Jović M, Milanović D. Effect of sauerkraut brine in central and peripheral LPS-induced  inflammation in C57BL/6 mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:68.
https://hdl.handle.net/21.15107/rcub_ibiss_5850 .

Vukojević, Anđela, Prvulović, Milica, Todorović, Smilja, Mladenović, Aleksandra, Sokanović, Srđan, Simeunović, Valentina, Jović, Milena, Milanović, Desanka, "Effect of sauerkraut brine in central and peripheral LPS-induced  inflammation in C57BL/6 mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):68,
https://hdl.handle.net/21.15107/rcub_ibiss_5850 .

TY  - CONF
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Dubljević, Olga
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5831
AB  - Aim: Microglia are macrophages of the brain with a fundamental role in neuronal
homeostasis. Stress-induced activation of microglia leads to rapid morphological
changes and it is implicated that these alternations could mediate different aspects of
early-life stress psychopathologies. We aimed to examine morphological response of
hippocampal microglia to social isolation using peripubertal rats as a rodent model of
adolescence.
Methods: Male peripubertal Wistar rats were randomly selected for group-housing
(GH; n=3 per cage) or single-housing (SH; n=1 per cage) at postnatal day 29. After
1st and 2nd week of defined housing animals῾ brains were sampled, and used for
microglia iba-1 fluorescent labelling and morphology analysis: one filed per section
per animal (n=4 animals per group) in hippocampal dentate gyrus (HDG). Data for
microglia cell shape (FracLac plugin, ImageJ) and cell ramification (Analyze Skeleton
plugin, ImageJ) were collected from single cells in photomicrograph visualized with a
confocal microscope (Leica Microsystems).
Results: Compared to GH peers, SH rats had lower microglial cell density in the HDG
after the 1st week of defined housing, which was not evident after the 2nd week. Other
parameters of microglia shape and ramification showed no difference between the
groups in neither of the two time points examined.
Conclusions: Obtained results showed that change in the cell density in the HDG was
the only and transient change in microglia morphology due to the social isolation
during adolescence. Further examination of other stress-related brain regions is
needed to define in depth the relationship between the microglia phenotype and earlylife
stress conditions.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Microglial morphological response to the lack of direct social contact in periadolescent rats
SP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5831
ER  - 

@conference{
author = "Potrebić, Milica and Pavković, Željko and Srbovan, Maja and Dubljević, Olga and Pešić, Vesna",
year = "2023",
abstract = "Aim: Microglia are macrophages of the brain with a fundamental role in neuronal
homeostasis. Stress-induced activation of microglia leads to rapid morphological
changes and it is implicated that these alternations could mediate different aspects of
early-life stress psychopathologies. We aimed to examine morphological response of
hippocampal microglia to social isolation using peripubertal rats as a rodent model of
adolescence.
Methods: Male peripubertal Wistar rats were randomly selected for group-housing
(GH; n=3 per cage) or single-housing (SH; n=1 per cage) at postnatal day 29. After
1st and 2nd week of defined housing animals῾ brains were sampled, and used for
microglia iba-1 fluorescent labelling and morphology analysis: one filed per section
per animal (n=4 animals per group) in hippocampal dentate gyrus (HDG). Data for
microglia cell shape (FracLac plugin, ImageJ) and cell ramification (Analyze Skeleton
plugin, ImageJ) were collected from single cells in photomicrograph visualized with a
confocal microscope (Leica Microsystems).
Results: Compared to GH peers, SH rats had lower microglial cell density in the HDG
after the 1st week of defined housing, which was not evident after the 2nd week. Other
parameters of microglia shape and ramification showed no difference between the
groups in neither of the two time points examined.
Conclusions: Obtained results showed that change in the cell density in the HDG was
the only and transient change in microglia morphology due to the social isolation
during adolescence. Further examination of other stress-related brain regions is
needed to define in depth the relationship between the microglia phenotype and earlylife
stress conditions.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Microglial morphological response to the lack of direct social contact in periadolescent rats",
pages = "108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5831"
}

Potrebić, M., Pavković, Ž., Srbovan, M., Dubljević, O.,& Pešić, V.. (2023). Microglial morphological response to the lack of direct social contact in periadolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 108.
https://hdl.handle.net/21.15107/rcub_ibiss_5831

Potrebić M, Pavković Ž, Srbovan M, Dubljević O, Pešić V. Microglial morphological response to the lack of direct social contact in periadolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:108.
https://hdl.handle.net/21.15107/rcub_ibiss_5831 .

Potrebić, Milica, Pavković, Željko, Srbovan, Maja, Dubljević, Olga, Pešić, Vesna, "Microglial morphological response to the lack of direct social contact in periadolescent rats" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):108,
https://hdl.handle.net/21.15107/rcub_ibiss_5831 .

TY  - CONF
AU  - Prvulović, Milica
AU  - Todorović, Smilja
AU  - Simeunović, Valentina
AU  - Vukojević, Anđela
AU  - Sokanović, Srđan
AU  - Jović, Milena
AU  - Milanović, Desanka
AU  - Mladenović, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5840
AB  - Caloric restriction (CR) is widely known for delaying age-related changes, but its 
duration and onset can significantly alter its protective potential. One of the 
underlying mechanisms of the effect of CR on aging is considered to be mTOR 
pathway, an important player in nutrient sensing and regulation of cellular 
metabolism.
Adult, middle-aged, and old male Wistar rats were exposed to CR (60% of AL) to 
study the effect of CR of early (EOCR) and late onset (LOCR). Ad libitum (AL) fed 
animals were used to test the effects of aging and as age-matched controls for the 
effect of CR. Western blot was used to analyze the expression of proteins involved in 
the signaling cascade of mTOR complex 1 (mTORC1) in rat hippocampus. 
At 18 months of age, EOCR led to a decrease in phosphorylation of mTOR S2448, 
whereas LOCR led to its increase at 24 months of age. pAkt T308 was decreased with 
LOCR at 18 months, while at 24 months EOCR and LOCR increased its 
phosphorylation. LOCR also led to an increased phosphorylation of p70S6K1 at 24 
months of age. 
Although EOCR led to a decrease in phosphorylation of mTOR at 18 months of age, 
this effect was lost at 24 months, and was also not affecting the activation of the two 
kinases targeting mTOR S2448. Negative effect of short-term LOCR at 24 months 
was consistent in all examined proteins suggesting a caution when implementing CR 
later in life.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - MTORC1 signaling pathway changes under the effect of caloric  restriction in the hippocampus of male Wistar rats
SP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5840
ER  - 

@conference{
author = "Prvulović, Milica and Todorović, Smilja and Simeunović, Valentina and Vukojević, Anđela and Sokanović, Srđan and Jović, Milena and Milanović, Desanka and Mladenović, Aleksandra",
year = "2023",
abstract = "Caloric restriction (CR) is widely known for delaying age-related changes, but its 
duration and onset can significantly alter its protective potential. One of the 
underlying mechanisms of the effect of CR on aging is considered to be mTOR 
pathway, an important player in nutrient sensing and regulation of cellular 
metabolism.
Adult, middle-aged, and old male Wistar rats were exposed to CR (60% of AL) to 
study the effect of CR of early (EOCR) and late onset (LOCR). Ad libitum (AL) fed 
animals were used to test the effects of aging and as age-matched controls for the 
effect of CR. Western blot was used to analyze the expression of proteins involved in 
the signaling cascade of mTOR complex 1 (mTORC1) in rat hippocampus. 
At 18 months of age, EOCR led to a decrease in phosphorylation of mTOR S2448, 
whereas LOCR led to its increase at 24 months of age. pAkt T308 was decreased with 
LOCR at 18 months, while at 24 months EOCR and LOCR increased its 
phosphorylation. LOCR also led to an increased phosphorylation of p70S6K1 at 24 
months of age. 
Although EOCR led to a decrease in phosphorylation of mTOR at 18 months of age, 
this effect was lost at 24 months, and was also not affecting the activation of the two 
kinases targeting mTOR S2448. Negative effect of short-term LOCR at 24 months 
was consistent in all examined proteins suggesting a caution when implementing CR 
later in life.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "MTORC1 signaling pathway changes under the effect of caloric  restriction in the hippocampus of male Wistar rats",
pages = "74",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5840"
}

Prvulović, M., Todorović, S., Simeunović, V., Vukojević, A., Sokanović, S., Jović, M., Milanović, D.,& Mladenović, A.. (2023). MTORC1 signaling pathway changes under the effect of caloric  restriction in the hippocampus of male Wistar rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 74.
https://hdl.handle.net/21.15107/rcub_ibiss_5840

Prvulović M, Todorović S, Simeunović V, Vukojević A, Sokanović S, Jović M, Milanović D, Mladenović A. MTORC1 signaling pathway changes under the effect of caloric  restriction in the hippocampus of male Wistar rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:74.
https://hdl.handle.net/21.15107/rcub_ibiss_5840 .

Prvulović, Milica, Todorović, Smilja, Simeunović, Valentina, Vukojević, Anđela, Sokanović, Srđan, Jović, Milena, Milanović, Desanka, Mladenović, Aleksandra, "MTORC1 signaling pathway changes under the effect of caloric  restriction in the hippocampus of male Wistar rats" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):74,
https://hdl.handle.net/21.15107/rcub_ibiss_5840 .

TY  - JOUR
AU  - Dubljević, Olga
AU  - Ković, Vanja
AU  - Pavković, Željko
AU  - Mitić, Miloš
AU  - Pešić, Vesna
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9599889
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5178
AB  - A potential relationship between unrestricted sucrose intake (USI), overweight, and emotional/behavioral control has not been well documented. We examined the influence of USI and having less sweetness than expected on body weight (BW), motor/exploratory, anxiety-like, and social dominant behavior in adult C57BL/6J male mice. Animals had free access to water (group 1) or 32% sucrose and water (sucrose groups 2-5) for 10 days. Then, group 2 remained with 32% sucrose while groups 3-5 were subjected to the downshift (24 h access to 4%, 8%, or 16% sucrose). All experimental groups were weighed and tested in the novel-open arena (NA), elevated plus maze (EPM), and tube tests to assess BW, motor/exploratory, anxiety-like, and social dominance behavior, respectively. USI did not influence animals' BW but produced hyperactivity and anxiolytic-like behavior, which was evident in EPM but not in NA; the outcomes of the downshift were comparable. USI did not influence successes/wins in the tube test but altered emotions that drive the winning, favoring a less anxious behavioral phenotype; this was not evident in the downshifted groups. Observed findings suggest that USI promotes sensation-seeking and motivates dominance, without changing BW, while blunted emotional base of social dominance might be an early mark of the downshift.
PB  - Basel: MDPI
T2  - Brain Sciences
T1  - The Influence of Unlimited Sucrose Intake on Body Weight and Behavior-Findings from a Mouse Model
IS  - 10
VL  - 12
DO  - 10.3390/brainsci12101332
SP  - 1332
ER  - 

@article{
author = "Dubljević, Olga and Ković, Vanja and Pavković, Željko and Mitić, Miloš and Pešić, Vesna",
year = "2022",
abstract = "A potential relationship between unrestricted sucrose intake (USI), overweight, and emotional/behavioral control has not been well documented. We examined the influence of USI and having less sweetness than expected on body weight (BW), motor/exploratory, anxiety-like, and social dominant behavior in adult C57BL/6J male mice. Animals had free access to water (group 1) or 32% sucrose and water (sucrose groups 2-5) for 10 days. Then, group 2 remained with 32% sucrose while groups 3-5 were subjected to the downshift (24 h access to 4%, 8%, or 16% sucrose). All experimental groups were weighed and tested in the novel-open arena (NA), elevated plus maze (EPM), and tube tests to assess BW, motor/exploratory, anxiety-like, and social dominance behavior, respectively. USI did not influence animals' BW but produced hyperactivity and anxiolytic-like behavior, which was evident in EPM but not in NA; the outcomes of the downshift were comparable. USI did not influence successes/wins in the tube test but altered emotions that drive the winning, favoring a less anxious behavioral phenotype; this was not evident in the downshifted groups. Observed findings suggest that USI promotes sensation-seeking and motivates dominance, without changing BW, while blunted emotional base of social dominance might be an early mark of the downshift.",
publisher = "Basel: MDPI",
journal = "Brain Sciences",
title = "The Influence of Unlimited Sucrose Intake on Body Weight and Behavior-Findings from a Mouse Model",
number = "10",
volume = "12",
doi = "10.3390/brainsci12101332",
pages = "1332"
}

Dubljević, O., Ković, V., Pavković, Ž., Mitić, M.,& Pešić, V.. (2022). The Influence of Unlimited Sucrose Intake on Body Weight and Behavior-Findings from a Mouse Model. in Brain Sciences
Basel: MDPI., 12(10), 1332.
https://doi.org/10.3390/brainsci12101332

Dubljević O, Ković V, Pavković Ž, Mitić M, Pešić V. The Influence of Unlimited Sucrose Intake on Body Weight and Behavior-Findings from a Mouse Model. in Brain Sciences. 2022;12(10):1332.
doi:10.3390/brainsci12101332 .

Dubljević, Olga, Ković, Vanja, Pavković, Željko, Mitić, Miloš, Pešić, Vesna, "The Influence of Unlimited Sucrose Intake on Body Weight and Behavior-Findings from a Mouse Model" in Brain Sciences, 12, no. 10 (2022):1332,
https://doi.org/10.3390/brainsci12101332 . .

TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Šobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1756464622003723
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5174
AB  - The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.
T2  - Journal of Functional Foods
T1  - The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice
VL  - 99
DO  - 10.1016/j.jff.2022.105302
SP  - 105302
ER  - 

@article{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Šobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.",
journal = "Journal of Functional Foods",
title = "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice",
volume = "99",
doi = "10.1016/j.jff.2022.105302",
pages = "105302"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Šobajić, S., Kanazir, S.,& Ivković, S.. (2022). The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods, 99, 105302.
https://doi.org/10.1016/j.jff.2022.105302

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Šobajić S, Kanazir S, Ivković S. The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods. 2022;99:105302.
doi:10.1016/j.jff.2022.105302 .

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Šobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice" in Journal of Functional Foods, 99 (2022):105302,
https://doi.org/10.1016/j.jff.2022.105302 . .

TY  - JOUR
AU  - Radovanović, Jelena
AU  - Antonijević, Biljana
AU  - Ćurčić, Marijana
AU  - Baralić, Katarina
AU  - Kolarević, Stoimir
AU  - Bulat, Zorica
AU  - Đukić-Ćosić, Danijela
AU  - Buha Đorđević, Aleksandra
AU  - Vuković-Gačić, Branka
AU  - Javorac, Dragana
AU  - Antonijević Miljaković, Evica
AU  - Carević, Momir
AU  - Mandinić, Zoran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5166
AB  - Excessive fluoride (F-) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F- subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F-; 3) 25 mg/L F-; 4) 50 mg/L F-; 5) 100 mg/L F-; 6) 150 mg/L F-. Testicles were dissected out and processed for the determination of F- tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups. The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F-/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F-, this dose-response study is valuable for further research regarding the F- health risk assessment.
PB  - Elsevier Ltd.
T2  - Environmental Pollution
T1  - Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage.
VL  - 314
DO  - 10.1016/j.envpol.2022.120321
SP  - 120321
ER  - 

@article{
author = "Radovanović, Jelena and Antonijević, Biljana and Ćurčić, Marijana and Baralić, Katarina and Kolarević, Stoimir and Bulat, Zorica and Đukić-Ćosić, Danijela and Buha Đorđević, Aleksandra and Vuković-Gačić, Branka and Javorac, Dragana and Antonijević Miljaković, Evica and Carević, Momir and Mandinić, Zoran",
year = "2022",
abstract = "Excessive fluoride (F-) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F- subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F-; 3) 25 mg/L F-; 4) 50 mg/L F-; 5) 100 mg/L F-; 6) 150 mg/L F-. Testicles were dissected out and processed for the determination of F- tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups. The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F-/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F-, this dose-response study is valuable for further research regarding the F- health risk assessment.",
publisher = "Elsevier Ltd.",
journal = "Environmental Pollution",
title = "Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage.",
volume = "314",
doi = "10.1016/j.envpol.2022.120321",
pages = "120321"
}

Radovanović, J., Antonijević, B., Ćurčić, M., Baralić, K., Kolarević, S., Bulat, Z., Đukić-Ćosić, D., Buha Đorđević, A., Vuković-Gačić, B., Javorac, D., Antonijević Miljaković, E., Carević, M.,& Mandinić, Z.. (2022). Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage.. in Environmental Pollution
Elsevier Ltd.., 314, 120321.
https://doi.org/10.1016/j.envpol.2022.120321

Radovanović J, Antonijević B, Ćurčić M, Baralić K, Kolarević S, Bulat Z, Đukić-Ćosić D, Buha Đorđević A, Vuković-Gačić B, Javorac D, Antonijević Miljaković E, Carević M, Mandinić Z. Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage.. in Environmental Pollution. 2022;314:120321.
doi:10.1016/j.envpol.2022.120321 .

Radovanović, Jelena, Antonijević, Biljana, Ćurčić, Marijana, Baralić, Katarina, Kolarević, Stoimir, Bulat, Zorica, Đukić-Ćosić, Danijela, Buha Đorđević, Aleksandra, Vuković-Gačić, Branka, Javorac, Dragana, Antonijević Miljaković, Evica, Carević, Momir, Mandinić, Zoran, "Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage." in Environmental Pollution, 314 (2022):120321,
https://doi.org/10.1016/j.envpol.2022.120321 . .

TY  - JOUR
AU  - Zavišić, Gordana
AU  - Ristić, Slavica
AU  - Rikalović, Milena
AU  - Petković, Branka
AU  - Janković, Drina
AU  - Vukadinović, Aleksandar
AU  - Petričević, Saša
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1756464622002377
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5091
AB  - The present study aimed to examine the effect of Lactobacillus rhamnosus Rosell 11 and Lactobacillus helveticus Rosell 52 on glucose (blood level and tolerance), lipids (cholesterol and triglycerides), transaminases (AST and ALT), ALP, urea, and creatinine, along with body weight, food intake, liquid consumption, and gross pathology in a mouse model of metabolic syndrome. Male C57BL/6J mice were fed a high-fat high-sucrose diet and treated by oral gavage with a probiotic mixture in three different concentrations (107, 108, and 109 CFU/mL) once daily for 2 months. Probiotic supplementation, particularly at a concentration of 109 CFU, significantly decreased blood glucose and serum triglyceride levels, improved glucose tolerance, and promoted body weight loss in mice fed a high-fat high-sucrose diet. According to the obtained results, probiotic supplementation is useful for controlling glucose and triglyceride levels and could be used as an adjunctive therapeutic approach in patients with metabolic syndrome. © 2022
PB  - Amsterdam: Elsevier
T2  - Journal of Functional Foods
T1  - Beneficial effects of probiotic supplementation on glucose and triglycerides in a mouse model of metabolic syndrome
VL  - 95
DO  - 10.1016/j.jff.2022.105167
SP  - 105167
ER  - 

@article{
author = "Zavišić, Gordana and Ristić, Slavica and Rikalović, Milena and Petković, Branka and Janković, Drina and Vukadinović, Aleksandar and Petričević, Saša",
year = "2022",
abstract = "The present study aimed to examine the effect of Lactobacillus rhamnosus Rosell 11 and Lactobacillus helveticus Rosell 52 on glucose (blood level and tolerance), lipids (cholesterol and triglycerides), transaminases (AST and ALT), ALP, urea, and creatinine, along with body weight, food intake, liquid consumption, and gross pathology in a mouse model of metabolic syndrome. Male C57BL/6J mice were fed a high-fat high-sucrose diet and treated by oral gavage with a probiotic mixture in three different concentrations (107, 108, and 109 CFU/mL) once daily for 2 months. Probiotic supplementation, particularly at a concentration of 109 CFU, significantly decreased blood glucose and serum triglyceride levels, improved glucose tolerance, and promoted body weight loss in mice fed a high-fat high-sucrose diet. According to the obtained results, probiotic supplementation is useful for controlling glucose and triglyceride levels and could be used as an adjunctive therapeutic approach in patients with metabolic syndrome. © 2022",
publisher = "Amsterdam: Elsevier",
journal = "Journal of Functional Foods",
title = "Beneficial effects of probiotic supplementation on glucose and triglycerides in a mouse model of metabolic syndrome",
volume = "95",
doi = "10.1016/j.jff.2022.105167",
pages = "105167"
}

Zavišić, G., Ristić, S., Rikalović, M., Petković, B., Janković, D., Vukadinović, A.,& Petričević, S.. (2022). Beneficial effects of probiotic supplementation on glucose and triglycerides in a mouse model of metabolic syndrome. in Journal of Functional Foods
Amsterdam: Elsevier., 95, 105167.
https://doi.org/10.1016/j.jff.2022.105167

Zavišić G, Ristić S, Rikalović M, Petković B, Janković D, Vukadinović A, Petričević S. Beneficial effects of probiotic supplementation on glucose and triglycerides in a mouse model of metabolic syndrome. in Journal of Functional Foods. 2022;95:105167.
doi:10.1016/j.jff.2022.105167 .

Zavišić, Gordana, Ristić, Slavica, Rikalović, Milena, Petković, Branka, Janković, Drina, Vukadinović, Aleksandar, Petričević, Saša, "Beneficial effects of probiotic supplementation on glucose and triglycerides in a mouse model of metabolic syndrome" in Journal of Functional Foods, 95 (2022):105167,
https://doi.org/10.1016/j.jff.2022.105167 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Đorđević, Ana
AU  - Romić, Snježana
AU  - Pantelić, Marija
AU  - Korićanac, Goran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5023
AB  - A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is
known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance,
though it is evident that dry cow muscles may contain increased triglyceride content. The current study
was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in
dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2
equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples
collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for
concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ),
glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine
palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus
muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows.
Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin
1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin
resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of
signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to
this disposition.
PB  - Amsterdam: Elsevier Ltd.
T2  - Research in Veterinary Science
T1  - Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle
VL  - 150
DO  - 10.1016/j.rvsc.2022.04.012
SP  - 189
EP  - 194
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Vujanac, Ivan and Đorđević, Ana and Romić, Snježana and Pantelić, Marija and Korićanac, Goran",
year = "2022",
abstract = "A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is
known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance,
though it is evident that dry cow muscles may contain increased triglyceride content. The current study
was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in
dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2
equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples
collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for
concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ),
glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine
palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus
muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows.
Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin
1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin
resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of
signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to
this disposition.",
publisher = "Amsterdam: Elsevier Ltd.",
journal = "Research in Veterinary Science",
title = "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle",
volume = "150",
doi = "10.1016/j.rvsc.2022.04.012",
pages = "189-194"
}

Prodanović, R., Kirovski, D., Vujanac, I., Đorđević, A., Romić, S., Pantelić, M.,& Korićanac, G.. (2022). Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science
Amsterdam: Elsevier Ltd.., 150, 189-194.
https://doi.org/10.1016/j.rvsc.2022.04.012

Prodanović R, Kirovski D, Vujanac I, Đorđević A, Romić S, Pantelić M, Korićanac G. Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science. 2022;150:189-194.
doi:10.1016/j.rvsc.2022.04.012 .

Prodanović, Radiša, Kirovski, Danijela, Vujanac, Ivan, Đorđević, Ana, Romić, Snježana, Pantelić, Marija, Korićanac, Goran, "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle" in Research in Veterinary Science, 150 (2022):189-194,
https://doi.org/10.1016/j.rvsc.2022.04.012 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
AU  - Mitić-Ćulafić, Dragana
AU  - Dukanović, Stefana
AU  - Krstić, Aleksandar
AU  - Pavlica, Dušan
AU  - Vlajić, Tamara
AU  - Marković, Dejan
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/0972060X.2022.2040386
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4944
AB  - The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.
PB  - Abingdon: Taylor & Francis Ltd.
T2  - Journal of Essential Oil-Bearing Plants
T1  - Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes
IS  - 1
VL  - 25
DO  - 10.1080/0972060X.2022.2040386
SP  - 111
EP  - 125
ER  - 

@article{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Ćirić, Ana and Mitić-Ćulafić, Dragana and Dukanović, Stefana and Krstić, Aleksandar and Pavlica, Dušan and Vlajić, Tamara and Marković, Dejan",
year = "2022",
abstract = "The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.",
publisher = "Abingdon: Taylor & Francis Ltd.",
journal = "Journal of Essential Oil-Bearing Plants",
title = "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes",
number = "1",
volume = "25",
doi = "10.1080/0972060X.2022.2040386",
pages = "111-125"
}

Marinković, J., Marković, T., Nikolić, B., Ćirić, A., Mitić-Ćulafić, D., Dukanović, S., Krstić, A., Pavlica, D., Vlajić, T.,& Marković, D.. (2022). Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants
Abingdon: Taylor & Francis Ltd.., 25(1), 111-125.
https://doi.org/10.1080/0972060X.2022.2040386

Marinković J, Marković T, Nikolić B, Ćirić A, Mitić-Ćulafić D, Dukanović S, Krstić A, Pavlica D, Vlajić T, Marković D. Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants. 2022;25(1):111-125.
doi:10.1080/0972060X.2022.2040386 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Ćirić, Ana, Mitić-Ćulafić, Dragana, Dukanović, Stefana, Krstić, Aleksandar, Pavlica, Dušan, Vlajić, Tamara, Marković, Dejan, "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes" in Journal of Essential Oil-Bearing Plants, 25, no. 1 (2022):111-125,
https://doi.org/10.1080/0972060X.2022.2040386 . .

TY  - JOUR
AU  - Ivković, Sanja
AU  - Major, Tamara
AU  - Mitić, Miloš
AU  - Lončarević-Vasiljković, Nataša
AU  - Jović, Milena
AU  - Adžić, Miroslav
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0024320522001709
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35283177
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4942
AB  - The brain is the softest organ in the body, and any change in the mechanical properties of the tissue induces the activation of glial cells, astrocytes and microglia. Amyloid plaques, one of the main pathological features of Alzheimer's disease (AD), are substantially harder than the surrounding brain tissue and can activate astrocytes and microglia resulting in the glial engulfment of plaques. Durotaxis, a migratory preference towards stiffer tissue, is prompting microglia to form a mechanical barrier around plaques reducing amyloid β (Aβ) induced neurotoxicity. Mechanoreceptors are highly expressed in the brain, particularly in microglia. The large increase in the expression of the mechanoreceptor Piezo1 was observed in the brains from AD animal models and AD patients in plaque encompassing glia. Importantly, Piezo1 function is regulated via force-from-lipids through the lipid composition of the membrane and membranous incorporation of polyunsaturated fatty acids (PUFAs) can affect the function of Piezo1 altering mechanosensitive properties of the cell. On the other hand, PUFAs dietary supplementation can alter microglial polarization, the envelopment of amyloid plaques, and immune response and Piezo1 activity was implicated in the similar modulations of microglia behavior. Finally, PUFAs treatment is currently in use in medical trials as the therapy for sickle cell anemia, a disease linked with the mutations in Piezo1. Further studies are needed to elucidate the connection between PUFAs, Piezo1 expression, and microglia behavior in the AD brain. These findings could open new possibilities in harnessing microglia in AD and in developing novel therapeutic strategies.
T2  - Life sciences
T1  - Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease.
VL  - 297
DO  - 10.1016/j.lfs.2022.120470
SP  - 120470
ER  - 

@article{
author = "Ivković, Sanja and Major, Tamara and Mitić, Miloš and Lončarević-Vasiljković, Nataša and Jović, Milena and Adžić, Miroslav",
year = "2022",
abstract = "The brain is the softest organ in the body, and any change in the mechanical properties of the tissue induces the activation of glial cells, astrocytes and microglia. Amyloid plaques, one of the main pathological features of Alzheimer's disease (AD), are substantially harder than the surrounding brain tissue and can activate astrocytes and microglia resulting in the glial engulfment of plaques. Durotaxis, a migratory preference towards stiffer tissue, is prompting microglia to form a mechanical barrier around plaques reducing amyloid β (Aβ) induced neurotoxicity. Mechanoreceptors are highly expressed in the brain, particularly in microglia. The large increase in the expression of the mechanoreceptor Piezo1 was observed in the brains from AD animal models and AD patients in plaque encompassing glia. Importantly, Piezo1 function is regulated via force-from-lipids through the lipid composition of the membrane and membranous incorporation of polyunsaturated fatty acids (PUFAs) can affect the function of Piezo1 altering mechanosensitive properties of the cell. On the other hand, PUFAs dietary supplementation can alter microglial polarization, the envelopment of amyloid plaques, and immune response and Piezo1 activity was implicated in the similar modulations of microglia behavior. Finally, PUFAs treatment is currently in use in medical trials as the therapy for sickle cell anemia, a disease linked with the mutations in Piezo1. Further studies are needed to elucidate the connection between PUFAs, Piezo1 expression, and microglia behavior in the AD brain. These findings could open new possibilities in harnessing microglia in AD and in developing novel therapeutic strategies.",
journal = "Life sciences",
title = "Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease.",
volume = "297",
doi = "10.1016/j.lfs.2022.120470",
pages = "120470"
}

Ivković, S., Major, T., Mitić, M., Lončarević-Vasiljković, N., Jović, M.,& Adžić, M.. (2022). Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease.. in Life sciences, 297, 120470.
https://doi.org/10.1016/j.lfs.2022.120470

Ivković S, Major T, Mitić M, Lončarević-Vasiljković N, Jović M, Adžić M. Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease.. in Life sciences. 2022;297:120470.
doi:10.1016/j.lfs.2022.120470 .

Ivković, Sanja, Major, Tamara, Mitić, Miloš, Lončarević-Vasiljković, Nataša, Jović, Milena, Adžić, Miroslav, "Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease." in Life sciences, 297 (2022):120470,
https://doi.org/10.1016/j.lfs.2022.120470 . .

TY  - CONF
AU  - Dubljević, Olga
AU  - Ković, Vanja
AU  - Pavković, Željko
AU  - Mitić, Miloš
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5617
AB  - Већина доступних животињских модела зависности од шећера не укључује компоненту само-администрирања супстанце, а познато је да се код људи конзумирање шећера одвија у ad libitum условима. Како би превазишли овај проблем, у овој студији испитани су конзуматорни и бихевиорални исходи неограниченог уноса шећера, као и накнадног смањења доступности шећера код адултних мужјака C57BL/6J мишева применом модификоване парадигме „Конзуматорни сукцесивни негативни контраст“ (cSNC).1,2 Две групе животиња имале су неограничен приступ 32% раствору сахарозе 10 дана (у виду тзв. Two- Bottle-Choice протокола); 11. дана једна група добила је раствор са мањом концентрацијом сахарозе (32%→4%), док је друга група добила уобичајени 32% раствор. Контролна група је имала ad libitum приступ води. Дванаестог дана стандардизованим тестовима понашања (Тест отвореног поља, Крстасти лавиринт и Туба тест социјалне конфронтације) испитани су социјална доминација, као и новином-изазвано експлораторно и понашање слично анксиозности. Добијени резултати указују на централни утицај неограниченог уноса шећера с обзиром да је код C57BL/6J мужјака уочен анксиолитички ефекат на понашање који вероватно одражава физиолошку дисрегулацију и склоност тражењу сензација. Потенцијални простор за примену нове модификоване парадигме у испитивању патолошких последица неограниченог уноса шећера даље је размотрен.
AB  - Većina dostupnih životinjskih modela zavisnosti od šećera ne uključuje komponentu samo-administriranja supstance, a poznato je da se kod ljudi konzumiranje šećera odvija u ad libitum uslovima. Kako bi prevazišli ovaj problem, u ovoj studiji ispitani su konzumatorni i bihevioralni ishodi neograničenog unosa šećera, kao i naknadnog smanjenja dostupnosti šećera kod adultnih mužjaka C57BL/6J miševa primenom modifikovane paradigme „Konzumatorni sukcesivni negativni kontrast“ (cSNC).1,2 Dve grupe životinja imale su neograničen pristup 32% rastvoru saharoze 10 dana (u vidu tzv. Two- Bottle-Choice protokola); 11. dana jedna grupa dobila je rastvor sa manjom koncentracijom saharoze (32%→4%), dok je druga grupa dobila uobičajeni 32% rastvor. Kontrolna grupa je imala ad libitum pristup vodi. Dvanaestog dana standardizovanim testovima ponašanja (Test otvorenog polja, Krstasti lavirint i Tuba test socijalne konfrontacije) ispitani su socijalna dominacija, kao i novinom-izazvano eksploratorno i ponašanje slično anksioznosti. Dobijeni rezultati ukazuju na centralni uticaj neograničenog unosa šećera s obzirom da je kod C57BL/6J mužjaka uočen anksiolitički efekat na ponašanje koji verovatno odražava fiziološku disregulaciju i sklonost traženju senzacija. Potencijalni prostor za primenu nove modifikovane paradigme u ispitivanju patoloških posledica neograničenog unosa šećera dalje je razmotren.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Конзуматорне и бихевиоралне последице неограниченог уноса шећера и  накнадног смањења доступности шећера
T1  - Konzumatorne i bihevioralne posledice neograničenog unosa šećera i naknadnog smanjenja dostupnosti šećera
SP  - 384
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5617
ER  - 

@conference{
author = "Dubljević, Olga and Ković, Vanja and Pavković, Željko and Mitić, Miloš and Pešić, Vesna",
year = "2022",
abstract = "Већина доступних животињских модела зависности од шећера не укључује компоненту само-администрирања супстанце, а познато је да се код људи конзумирање шећера одвија у ad libitum условима. Како би превазишли овај проблем, у овој студији испитани су конзуматорни и бихевиорални исходи неограниченог уноса шећера, као и накнадног смањења доступности шећера код адултних мужјака C57BL/6J мишева применом модификоване парадигме „Конзуматорни сукцесивни негативни контраст“ (cSNC).1,2 Две групе животиња имале су неограничен приступ 32% раствору сахарозе 10 дана (у виду тзв. Two- Bottle-Choice протокола); 11. дана једна група добила је раствор са мањом концентрацијом сахарозе (32%→4%), док је друга група добила уобичајени 32% раствор. Контролна група је имала ad libitum приступ води. Дванаестог дана стандардизованим тестовима понашања (Тест отвореног поља, Крстасти лавиринт и Туба тест социјалне конфронтације) испитани су социјална доминација, као и новином-изазвано експлораторно и понашање слично анксиозности. Добијени резултати указују на централни утицај неограниченог уноса шећера с обзиром да је код C57BL/6J мужјака уочен анксиолитички ефекат на понашање који вероватно одражава физиолошку дисрегулацију и склоност тражењу сензација. Потенцијални простор за примену нове модификоване парадигме у испитивању патолошких последица неограниченог уноса шећера даље је размотрен., Većina dostupnih životinjskih modela zavisnosti od šećera ne uključuje komponentu samo-administriranja supstance, a poznato je da se kod ljudi konzumiranje šećera odvija u ad libitum uslovima. Kako bi prevazišli ovaj problem, u ovoj studiji ispitani su konzumatorni i bihevioralni ishodi neograničenog unosa šećera, kao i naknadnog smanjenja dostupnosti šećera kod adultnih mužjaka C57BL/6J miševa primenom modifikovane paradigme „Konzumatorni sukcesivni negativni kontrast“ (cSNC).1,2 Dve grupe životinja imale su neograničen pristup 32% rastvoru saharoze 10 dana (u vidu tzv. Two- Bottle-Choice protokola); 11. dana jedna grupa dobila je rastvor sa manjom koncentracijom saharoze (32%→4%), dok je druga grupa dobila uobičajeni 32% rastvor. Kontrolna grupa je imala ad libitum pristup vodi. Dvanaestog dana standardizovanim testovima ponašanja (Test otvorenog polja, Krstasti lavirint i Tuba test socijalne konfrontacije) ispitani su socijalna dominacija, kao i novinom-izazvano eksploratorno i ponašanje slično anksioznosti. Dobijeni rezultati ukazuju na centralni uticaj neograničenog unosa šećera s obzirom da je kod C57BL/6J mužjaka uočen anksiolitički efekat na ponašanje koji verovatno odražava fiziološku disregulaciju i sklonost traženju senzacija. Potencijalni prostor za primenu nove modifikovane paradigme u ispitivanju patoloških posledica neograničenog unosa šećera dalje je razmotren.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Конзуматорне и бихевиоралне последице неограниченог уноса шећера и  накнадног смањења доступности шећера, Konzumatorne i bihevioralne posledice neograničenog unosa šećera i naknadnog smanjenja dostupnosti šećera",
pages = "384",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5617"
}

Dubljević, O., Ković, V., Pavković, Ž., Mitić, M.,& Pešić, V.. (2022). Конзуматорне и бихевиоралне последице неограниченог уноса шећера и  накнадног смањења доступности шећера. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 384.
https://hdl.handle.net/21.15107/rcub_ibiss_5617

Dubljević O, Ković V, Pavković Ž, Mitić M, Pešić V. Конзуматорне и бихевиоралне последице неограниченог уноса шећера и  накнадног смањења доступности шећера. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:384.
https://hdl.handle.net/21.15107/rcub_ibiss_5617 .

Dubljević, Olga, Ković, Vanja, Pavković, Željko, Mitić, Miloš, Pešić, Vesna, "Конзуматорне и бихевиоралне последице неограниченог уноса шећера и  накнадног смањења доступности шећера" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):384,
https://hdl.handle.net/21.15107/rcub_ibiss_5617 .

TY  - CONF
AU  - Nikolić, Biljana
AU  - Marković, Dejan
AU  - Marković, Tatjana
AU  - Mitić-Ćulafić, Dragana
AU  - Vuletić, Stefana
AU  - Cvetković, Stefana
AU  - Ćirić, Ana
AU  - Marinković, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5584
AB  - Mикробиота усне дупље обухвата више стотина/хиљада врста са примарном
функцијом заштите организма од колонизације патогенима. Међутим, ако
осетљиви екосистем усне дупље изађе из равнотеже, услед пада имунитета и/или
пренамножености микроорганизама у биофилму (денталном плаку), развијају се
болести зуба (каријес, инфекције зубне пулпе) или периодонтална обољења, што
чак може угрозити системско здравље. Имајући то у виду, лечење зубних
инфекција је од изузетног значаја, а потреба за развојем нових антимикробних
агенаса/процедура евидентна. Пошто су етарска уља (ЕУ) позната по снажном
антибактеријском/антибиофилм ефекту, фокусирали смо се на истраживање
њихове потенцијалне примене у лечењу инфекција коренских канала зуба. Из
инфицираних канала изоловани су бројни факултативни и стриктни анаероби,1 али
је истраживање усмерено на Enterococcus faecalis, као најчешћег узрочника
перзистентних/рекурентних инфекција. Иако смо антибактеријски ефекат
детектовали за бројна ЕУ, детаљна истраживања су спроведена са уљима Thymus
zygis, Cymbopogon martinii2 и C. citratus3. Адекватна испорука ЕУ у коренски канал
подразумевала је формулацију микро- и наноемулзија. Истраживање је спроведено
in vitro и на моно- (E. faecalis) и мултиспецијском биофилму у каналима
екстрахованих зуба. У случају C. martinii оптимизована је и процедура припреме
ефикасних наноемулзија. Добијени резултати су обећавајући: емулзије ЕУ се
успешно могу користити као допунска терапија инфицираних коренских канала
зуба.
AB  - Mikrobiota usne duplje obuhvata više stotina/hiljada vrsta sa primarnom funkcijom zaštite organizma od kolonizacije patogenima. Međutim, ako osetljivi ekosistem usne duplje izađe iz ravnoteže, usled pada imuniteta i/ili prenamnoženosti mikroorganizama u biofilmu (dentalnom plaku), razvijaju se bolesti zuba (karijes, infekcije zubne pulpe) ili periodontalna oboljenja, što čak može ugroziti sistemsko zdravlje. Imajući to u vidu, lečenje zubnih infekcija je od izuzetnog značaja, a potreba za razvojem novih antimikrobnih agenasa/procedura evidentna. Pošto su etarska ulja (EU) poznata po snažnom antibakterijskom/antibiofilm efektu, fokusirali smo se na istraživanje njihove potencijalne primene u lečenju infekcija korenskih kanala zuba. Iz inficiranih kanala izolovani su brojni fakultativni i striktni anaerobi,1 ali je istraživanje usmereno na Enterococcus faecalis, kao najčešćeg uzročnika perzistentnih/rekurentnih infekcija. Iako smo antibakterijski efekat detektovali za brojna EU, detaljna istraživanja su sprovedena sa uljima Thymus zygis, Cymbopogon martinii2 i C. citratus3. Adekvatna isporuka EU u korenski kanal podrazumevala je formulaciju mikro- i nanoemulzija. Istraživanje je sprovedeno in vitro i na mono- (E. faecalis) i multispecijskom biofilmu u kanalima ekstrahovanih zuba. U slučaju C. martinii optimizovana je i procedura pripreme efikasnih nanoemulzija. Dobijeni rezultati su obećavajući: emulzije EU se uspešno mogu koristiti kao dopunska terapija inficiranih korenskih kanala zuba.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Mogućnosti primene antibakterijskog i antibiofilmnog potencijala etarskih ulja u lečenju infekcija zuba
T1  - Могућности примене антибактеријског и антибиофилмног потенцијала етарских уља у лечењу инфекција зуба
SP  - 4
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5584
ER  - 

@conference{
author = "Nikolić, Biljana and Marković, Dejan and Marković, Tatjana and Mitić-Ćulafić, Dragana and Vuletić, Stefana and Cvetković, Stefana and Ćirić, Ana and Marinković, Jelena",
year = "2022",
abstract = "Mикробиота усне дупље обухвата више стотина/хиљада врста са примарном
функцијом заштите организма од колонизације патогенима. Међутим, ако
осетљиви екосистем усне дупље изађе из равнотеже, услед пада имунитета и/или
пренамножености микроорганизама у биофилму (денталном плаку), развијају се
болести зуба (каријес, инфекције зубне пулпе) или периодонтална обољења, што
чак може угрозити системско здравље. Имајући то у виду, лечење зубних
инфекција је од изузетног значаја, а потреба за развојем нових антимикробних
агенаса/процедура евидентна. Пошто су етарска уља (ЕУ) позната по снажном
антибактеријском/антибиофилм ефекту, фокусирали смо се на истраживање
њихове потенцијалне примене у лечењу инфекција коренских канала зуба. Из
инфицираних канала изоловани су бројни факултативни и стриктни анаероби,1 али
је истраживање усмерено на Enterococcus faecalis, као најчешћег узрочника
перзистентних/рекурентних инфекција. Иако смо антибактеријски ефекат
детектовали за бројна ЕУ, детаљна истраживања су спроведена са уљима Thymus
zygis, Cymbopogon martinii2 и C. citratus3. Адекватна испорука ЕУ у коренски канал
подразумевала је формулацију микро- и наноемулзија. Истраживање је спроведено
in vitro и на моно- (E. faecalis) и мултиспецијском биофилму у каналима
екстрахованих зуба. У случају C. martinii оптимизована је и процедура припреме
ефикасних наноемулзија. Добијени резултати су обећавајући: емулзије ЕУ се
успешно могу користити као допунска терапија инфицираних коренских канала
зуба., Mikrobiota usne duplje obuhvata više stotina/hiljada vrsta sa primarnom funkcijom zaštite organizma od kolonizacije patogenima. Međutim, ako osetljivi ekosistem usne duplje izađe iz ravnoteže, usled pada imuniteta i/ili prenamnoženosti mikroorganizama u biofilmu (dentalnom plaku), razvijaju se bolesti zuba (karijes, infekcije zubne pulpe) ili periodontalna oboljenja, što čak može ugroziti sistemsko zdravlje. Imajući to u vidu, lečenje zubnih infekcija je od izuzetnog značaja, a potreba za razvojem novih antimikrobnih agenasa/procedura evidentna. Pošto su etarska ulja (EU) poznata po snažnom antibakterijskom/antibiofilm efektu, fokusirali smo se na istraživanje njihove potencijalne primene u lečenju infekcija korenskih kanala zuba. Iz inficiranih kanala izolovani su brojni fakultativni i striktni anaerobi,1 ali je istraživanje usmereno na Enterococcus faecalis, kao najčešćeg uzročnika perzistentnih/rekurentnih infekcija. Iako smo antibakterijski efekat detektovali za brojna EU, detaljna istraživanja su sprovedena sa uljima Thymus zygis, Cymbopogon martinii2 i C. citratus3. Adekvatna isporuka EU u korenski kanal podrazumevala je formulaciju mikro- i nanoemulzija. Istraživanje je sprovedeno in vitro i na mono- (E. faecalis) i multispecijskom biofilmu u kanalima ekstrahovanih zuba. U slučaju C. martinii optimizovana je i procedura pripreme efikasnih nanoemulzija. Dobijeni rezultati su obećavajući: emulzije EU se uspešno mogu koristiti kao dopunska terapija inficiranih korenskih kanala zuba.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Mogućnosti primene antibakterijskog i antibiofilmnog potencijala etarskih ulja u lečenju infekcija zuba, Могућности примене антибактеријског и антибиофилмног потенцијала етарских уља у лечењу инфекција зуба",
pages = "4",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5584"
}

Nikolić, B., Marković, D., Marković, T., Mitić-Ćulafić, D., Vuletić, S., Cvetković, S., Ćirić, A.,& Marinković, J.. (2022). Mogućnosti primene antibakterijskog i antibiofilmnog potencijala etarskih ulja u lečenju infekcija zuba. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 4.
https://hdl.handle.net/21.15107/rcub_ibiss_5584

Nikolić B, Marković D, Marković T, Mitić-Ćulafić D, Vuletić S, Cvetković S, Ćirić A, Marinković J. Mogućnosti primene antibakterijskog i antibiofilmnog potencijala etarskih ulja u lečenju infekcija zuba. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:4.
https://hdl.handle.net/21.15107/rcub_ibiss_5584 .

Nikolić, Biljana, Marković, Dejan, Marković, Tatjana, Mitić-Ćulafić, Dragana, Vuletić, Stefana, Cvetković, Stefana, Ćirić, Ana, Marinković, Jelena, "Mogućnosti primene antibakterijskog i antibiofilmnog potencijala etarskih ulja u lečenju infekcija zuba" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):4,
https://hdl.handle.net/21.15107/rcub_ibiss_5584 .

TY  - JOUR
AU  - Krunić, Matija
AU  - Ristić, Biljana
AU  - Bošnjak, Mihajlo
AU  - Paunović, Verica
AU  - Tovilović-Kovačević, Gordana
AU  - Zogović, Nevena
AU  - Mirčić, Aleksandar
AU  - Marković, Zoran
AU  - Todorović-Marković, Biljana
AU  - Jovanović, Svetlana
AU  - Kleut, Duška
AU  - Mojović, Miloš
AU  - Nakarada, Đura
AU  - Marković, Olivera
AU  - Vuković, Irena
AU  - Harhaji-Trajković, Ljubica
AU  - Trajković, Vladimir
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0891584921007760
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4655
AB  - We investigated the ability of graphene quantum dot (GQD) nanoparticles to protect SH-SY5Y human neuroblastoma cells from oxidative/nitrosative stress induced by iron-nitrosyl complex sodium nitroprusside (SNP). GQD reduced SNP cytotoxicity by preventing mitochondrial depolarization, caspase-2 activation, and subsequent apoptotic death. Although GQD diminished the levels of nitric oxide (NO) in SNP-exposed cells, NO scavengers displayed only a slight protective effect, suggesting that NO quenching was not the main protective mechanism of GQD. GQD also reduced SNP-triggered increase in the intracellular levels of hydroxyl radical (•OH), superoxide anion (O2•-), and lipid peroxidation. Nonselective antioxidants, •OH scavenging, and iron chelators, but not superoxide dismutase, mimicked GQD cytoprotective activity, indicating that GQD protect cells by neutralizing •OH generated in the presence of SNP-released iron. Cellular internalization of GQD was required for optimal protection, since a removal of extracellular GQD by extensive washing only partly diminished their protective effect. Moreover, GQD cooperated with SNP to induce autophagy, as confirmed by the inhibition of autophagy-limiting Akt/PRAS40/mTOR signaling and increase in autophagy gene transcription, protein levels of proautophagic beclin-1 and LC3-II, formation of autophagic vesicles, and degradation of autophagic target p62. The antioxidant activity of GQD was not involved in autophagy induction, as antioxidants N-acetylcysteine and dimethyl sulfoxide failed to stimulate autophagy in SNP-exposed cells. Pharmacological inhibitors of early (wortmannin, 3-methyladenine) or late stages of autophagy (NH4Cl) efficiently reduced the protective effect of GQD. Therefore, the ability of GQD to prevent the in vitro neurotoxicity of SNP depends on both •OH/NO scavenging and induction of cytoprotective autophagy.
PB  - Elsevier Inc.
T2  - Free Radical Biology and Medicine
T1  - Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.10.025
SP  - 167
EP  - 180
ER  - 

@article{
author = "Krunić, Matija and Ristić, Biljana and Bošnjak, Mihajlo and Paunović, Verica and Tovilović-Kovačević, Gordana and Zogović, Nevena and Mirčić, Aleksandar and Marković, Zoran and Todorović-Marković, Biljana and Jovanović, Svetlana and Kleut, Duška and Mojović, Miloš and Nakarada, Đura and Marković, Olivera and Vuković, Irena and Harhaji-Trajković, Ljubica and Trajković, Vladimir",
year = "2021",
abstract = "We investigated the ability of graphene quantum dot (GQD) nanoparticles to protect SH-SY5Y human neuroblastoma cells from oxidative/nitrosative stress induced by iron-nitrosyl complex sodium nitroprusside (SNP). GQD reduced SNP cytotoxicity by preventing mitochondrial depolarization, caspase-2 activation, and subsequent apoptotic death. Although GQD diminished the levels of nitric oxide (NO) in SNP-exposed cells, NO scavengers displayed only a slight protective effect, suggesting that NO quenching was not the main protective mechanism of GQD. GQD also reduced SNP-triggered increase in the intracellular levels of hydroxyl radical (•OH), superoxide anion (O2•-), and lipid peroxidation. Nonselective antioxidants, •OH scavenging, and iron chelators, but not superoxide dismutase, mimicked GQD cytoprotective activity, indicating that GQD protect cells by neutralizing •OH generated in the presence of SNP-released iron. Cellular internalization of GQD was required for optimal protection, since a removal of extracellular GQD by extensive washing only partly diminished their protective effect. Moreover, GQD cooperated with SNP to induce autophagy, as confirmed by the inhibition of autophagy-limiting Akt/PRAS40/mTOR signaling and increase in autophagy gene transcription, protein levels of proautophagic beclin-1 and LC3-II, formation of autophagic vesicles, and degradation of autophagic target p62. The antioxidant activity of GQD was not involved in autophagy induction, as antioxidants N-acetylcysteine and dimethyl sulfoxide failed to stimulate autophagy in SNP-exposed cells. Pharmacological inhibitors of early (wortmannin, 3-methyladenine) or late stages of autophagy (NH4Cl) efficiently reduced the protective effect of GQD. Therefore, the ability of GQD to prevent the in vitro neurotoxicity of SNP depends on both •OH/NO scavenging and induction of cytoprotective autophagy.",
publisher = "Elsevier Inc.",
journal = "Free Radical Biology and Medicine",
title = "Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.",
volume = "177",
doi = "10.1016/j.freeradbiomed.2021.10.025",
pages = "167-180"
}

Krunić, M., Ristić, B., Bošnjak, M., Paunović, V., Tovilović-Kovačević, G., Zogović, N., Mirčić, A., Marković, Z., Todorović-Marković, B., Jovanović, S., Kleut, D., Mojović, M., Nakarada, Đ., Marković, O., Vuković, I., Harhaji-Trajković, L.,& Trajković, V.. (2021). Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.. in Free Radical Biology and Medicine
Elsevier Inc.., 177, 167-180.
https://doi.org/10.1016/j.freeradbiomed.2021.10.025

Krunić M, Ristić B, Bošnjak M, Paunović V, Tovilović-Kovačević G, Zogović N, Mirčić A, Marković Z, Todorović-Marković B, Jovanović S, Kleut D, Mojović M, Nakarada Đ, Marković O, Vuković I, Harhaji-Trajković L, Trajković V. Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.. in Free Radical Biology and Medicine. 2021;177:167-180.
doi:10.1016/j.freeradbiomed.2021.10.025 .

Krunić, Matija, Ristić, Biljana, Bošnjak, Mihajlo, Paunović, Verica, Tovilović-Kovačević, Gordana, Zogović, Nevena, Mirčić, Aleksandar, Marković, Zoran, Todorović-Marković, Biljana, Jovanović, Svetlana, Kleut, Duška, Mojović, Miloš, Nakarada, Đura, Marković, Olivera, Vuković, Irena, Harhaji-Trajković, Ljubica, Trajković, Vladimir, "Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death." in Free Radical Biology and Medicine, 177 (2021):167-180,
https://doi.org/10.1016/j.freeradbiomed.2021.10.025 . .

TY  - JOUR
AU  - Despotović, Ana
AU  - Antonijević, Đorđe M
AU  - Ilić, Dragan
AU  - Zogović, Nevena
AU  - Jokanović, Vukoman R
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4430
AB  - Introduction Calcium silicate (CS) dental cements have numerous clinical indications in dentistry including pulp capping, root end surgery, perforation repair and apexification/apexogenesis treatment. Materials and methods Novel CS based dental cement with incorporation of SrCO3 radiopacifier named ALBO-DENT was used as an experimental cement material while Portland cement (Aalborg, Denmark) and ProRoot MTA (Tulsa Dental, USA) were used as controls. The radiopacity evaluation was performed using digital Trophy Radiographic system with an intention to precisely determine the minimum of radiopaque agent needed to confer to ISO radiopacity requirement. Thereafter, biocompatibility of material was tested in in vitro conditions in mouse fibrosarcoma L929 cell culture treated with materials’ extracts. Cell morphology was observed using phase-contrast microscopy, while cell viability was measured using crystal violet (CV) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. Results Radiopacity evaluation revealed that 30%wt addition of SrCO3 was necessary to achieve satisfactory radiopacity (3.45 mm Al). Cytotoxicity analysis using CV and MTT assays revealed that pure extracts of ALBO-DENT presented superior biocompatibility when compared to PC and MTA controls while serial dilutions of experimental cements’ extracts as well as that of PC and MTA did not influence L929 cell viability. Conclusions Novel formulation of CS cement – ALBO-DENT presented satisfactory radiopacity and adequate biocompatibility.
AB  - Uvod Kalcijum-silikatni (KS) dentalni cementi se koriste u brojnim kliničkim indikacijama u stomatologiji koje uključuju direktno prekrivanje pulpe, retrogradnu hirurgiju korena zuba, lečenje perforacija i apeksogenezu/apeksifikaciju. Materijali i metode U istraživanju je korišćen novosintetisani cement na bazi KS sa dodatkom SrCO3 kao kontrastnog agensa ALBO-DENTA, dok su kao kontrola korišćeni cement Portland (PC, Aalborg, Denmark) i ProRoot MTA (MTA, Tulsa Dental, USA). Rendgenokontrasnost je ispitivana digitalnom radiografijom primenom aparata Trophy, sa namerom da se precizno odredi minimum
kontrastnog agensa koji zadovoljava zahteve standarda ISO za rendgenkontrastnost. Biokompatibilnost materijala je ispitana in vitro, u kulturi ćelija mišjeg fibrosarkoma L929 tretiranoj ekstraktima ispitivanih materijala. Ćelijska morfologija je praćena upotrebom fazno-kontrastne mikroskopije, dok je vijabilnost ćelija utvrđivana kristal violet (KV) i 3-(4,5-dimetiltiazol-2-yl)-2,5-difenfl-tetrazolium bromid (MTT) esejima.
Rezultati Ispitivanje rendgenkontrastnosti je pokazalo da dodatak 30% SrCO3 dovodi do zadovoljavajućeg kontrasta materijala (3,45 mm Al). Analiza citotoksičnosti KV i MTT metodom je pokazala da čisti ekstrakt ALBO-DENTA pokazuje bolju biokompatibilnost u poređenju sa PC i MTA, dok serijska razblaženja ekstrakta ispitivanog cementa, kao i PC i MTA, nisu uticala na vijabilitet ćelija L929. Zaključci Novi cement na bazi KS – ALBO-DENT pokazao je zavodovoljavajuću rendgenkontrastnost i odgovarajuću biokompatibilnost.
PB  - Belgrade: Serbian Dental Journal
T2  - Serbian Dental Journal
T1  - Investigation of the radiopacity and cytotoxicity of ALBODENT – novel strontium carbonate incorporated calcium silicate based dental cement
T1  - Ispitivanje rendgenkontrastnosti i citotoksičnosti ALBO-DENTA – novog kalcijum-silikatnog cementa sa dodatkom stroncijumkarbonata
IS  - 2
VL  - 68
DO  - 10.2298/SGS2102068D
SP  - 68
EP  - 78
ER  - 

@article{
author = "Despotović, Ana and Antonijević, Đorđe M and Ilić, Dragan and Zogović, Nevena and Jokanović, Vukoman R",
year = "2021",
abstract = "Introduction Calcium silicate (CS) dental cements have numerous clinical indications in dentistry including pulp capping, root end surgery, perforation repair and apexification/apexogenesis treatment. Materials and methods Novel CS based dental cement with incorporation of SrCO3 radiopacifier named ALBO-DENT was used as an experimental cement material while Portland cement (Aalborg, Denmark) and ProRoot MTA (Tulsa Dental, USA) were used as controls. The radiopacity evaluation was performed using digital Trophy Radiographic system with an intention to precisely determine the minimum of radiopaque agent needed to confer to ISO radiopacity requirement. Thereafter, biocompatibility of material was tested in in vitro conditions in mouse fibrosarcoma L929 cell culture treated with materials’ extracts. Cell morphology was observed using phase-contrast microscopy, while cell viability was measured using crystal violet (CV) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. Results Radiopacity evaluation revealed that 30%wt addition of SrCO3 was necessary to achieve satisfactory radiopacity (3.45 mm Al). Cytotoxicity analysis using CV and MTT assays revealed that pure extracts of ALBO-DENT presented superior biocompatibility when compared to PC and MTA controls while serial dilutions of experimental cements’ extracts as well as that of PC and MTA did not influence L929 cell viability. Conclusions Novel formulation of CS cement – ALBO-DENT presented satisfactory radiopacity and adequate biocompatibility., Uvod Kalcijum-silikatni (KS) dentalni cementi se koriste u brojnim kliničkim indikacijama u stomatologiji koje uključuju direktno prekrivanje pulpe, retrogradnu hirurgiju korena zuba, lečenje perforacija i apeksogenezu/apeksifikaciju. Materijali i metode U istraživanju je korišćen novosintetisani cement na bazi KS sa dodatkom SrCO3 kao kontrastnog agensa ALBO-DENTA, dok su kao kontrola korišćeni cement Portland (PC, Aalborg, Denmark) i ProRoot MTA (MTA, Tulsa Dental, USA). Rendgenokontrasnost je ispitivana digitalnom radiografijom primenom aparata Trophy, sa namerom da se precizno odredi minimum
kontrastnog agensa koji zadovoljava zahteve standarda ISO za rendgenkontrastnost. Biokompatibilnost materijala je ispitana in vitro, u kulturi ćelija mišjeg fibrosarkoma L929 tretiranoj ekstraktima ispitivanih materijala. Ćelijska morfologija je praćena upotrebom fazno-kontrastne mikroskopije, dok je vijabilnost ćelija utvrđivana kristal violet (KV) i 3-(4,5-dimetiltiazol-2-yl)-2,5-difenfl-tetrazolium bromid (MTT) esejima.
Rezultati Ispitivanje rendgenkontrastnosti je pokazalo da dodatak 30% SrCO3 dovodi do zadovoljavajućeg kontrasta materijala (3,45 mm Al). Analiza citotoksičnosti KV i MTT metodom je pokazala da čisti ekstrakt ALBO-DENTA pokazuje bolju biokompatibilnost u poređenju sa PC i MTA, dok serijska razblaženja ekstrakta ispitivanog cementa, kao i PC i MTA, nisu uticala na vijabilitet ćelija L929. Zaključci Novi cement na bazi KS – ALBO-DENT pokazao je zavodovoljavajuću rendgenkontrastnost i odgovarajuću biokompatibilnost.",
publisher = "Belgrade: Serbian Dental Journal",
journal = "Serbian Dental Journal",
title = "Investigation of the radiopacity and cytotoxicity of ALBODENT – novel strontium carbonate incorporated calcium silicate based dental cement, Ispitivanje rendgenkontrastnosti i citotoksičnosti ALBO-DENTA – novog kalcijum-silikatnog cementa sa dodatkom stroncijumkarbonata",
number = "2",
volume = "68",
doi = "10.2298/SGS2102068D",
pages = "68-78"
}

Despotović, A., Antonijević, Đ. M., Ilić, D., Zogović, N.,& Jokanović, V. R.. (2021). Investigation of the radiopacity and cytotoxicity of ALBODENT – novel strontium carbonate incorporated calcium silicate based dental cement. in Serbian Dental Journal
Belgrade: Serbian Dental Journal., 68(2), 68-78.
https://doi.org/10.2298/SGS2102068D

Despotović A, Antonijević ĐM, Ilić D, Zogović N, Jokanović VR. Investigation of the radiopacity and cytotoxicity of ALBODENT – novel strontium carbonate incorporated calcium silicate based dental cement. in Serbian Dental Journal. 2021;68(2):68-78.
doi:10.2298/SGS2102068D .

Despotović, Ana, Antonijević, Đorđe M, Ilić, Dragan, Zogović, Nevena, Jokanović, Vukoman R, "Investigation of the radiopacity and cytotoxicity of ALBODENT – novel strontium carbonate incorporated calcium silicate based dental cement" in Serbian Dental Journal, 68, no. 2 (2021):68-78,
https://doi.org/10.2298/SGS2102068D . .

TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Despotović, Ana
AU  - Biočanin, Vladimir
AU  - Milošević, Miloš
AU  - Trišić, Dijana
AU  - Lazović, Vladimir
AU  - Zogović, Nevena
AU  - Milašin, Jelena
AU  - Ilić, Dragan
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0272884221020794
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4464
AB  - The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p < 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p < 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p < 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p < 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p < 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements’ surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.
PB  - Oxford : Elsevier
T2  - Ceramics International
T1  - Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic
IS  - 20
VL  - 47
DO  - 10.1016/j.ceramint.2021.07.052
SP  - 28913
EP  - 28923
ER  - 

@article{
author = "Antonijević, Đorđe and Despotović, Ana and Biočanin, Vladimir and Milošević, Miloš and Trišić, Dijana and Lazović, Vladimir and Zogović, Nevena and Milašin, Jelena and Ilić, Dragan",
year = "2021",
abstract = "The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p < 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p < 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p < 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p < 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p < 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements’ surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.",
publisher = "Oxford : Elsevier",
journal = "Ceramics International",
title = "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic",
number = "20",
volume = "47",
doi = "10.1016/j.ceramint.2021.07.052",
pages = "28913-28923"
}

Antonijević, Đ., Despotović, A., Biočanin, V., Milošević, M., Trišić, D., Lazović, V., Zogović, N., Milašin, J.,& Ilić, D.. (2021). Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International
Oxford : Elsevier., 47(20), 28913-28923.
https://doi.org/10.1016/j.ceramint.2021.07.052

Antonijević Đ, Despotović A, Biočanin V, Milošević M, Trišić D, Lazović V, Zogović N, Milašin J, Ilić D. Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International. 2021;47(20):28913-28923.
doi:10.1016/j.ceramint.2021.07.052 .

Antonijević, Đorđe, Despotović, Ana, Biočanin, Vladimir, Milošević, Miloš, Trišić, Dijana, Lazović, Vladimir, Zogović, Nevena, Milašin, Jelena, Ilić, Dragan, "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic" in Ceramics International, 47, no. 20 (2021):28913-28923,
https://doi.org/10.1016/j.ceramint.2021.07.052 . .

TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Despotović, Ana
AU  - Biočanin, Vladimir
AU  - Milošević, Miloš
AU  - Trišić, Dijana
AU  - Lazović, Vladimir
AU  - Zogović, Nevena
AU  - Milašin, Jelena
AU  - Ilić, Dragan
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0272884221020794
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4445
AB  - The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p < 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p < 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p < 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p < 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p < 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements’ surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.
PB  - Oxford: Elsevier Ltd
T2  - Ceramics International
T1  - Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic
IS  - 20
VL  - 47
DO  - 10.1016/j.ceramint.2021.07.052
SP  - 28913
EP  - 28923
ER  - 

@article{
author = "Antonijević, Đorđe and Despotović, Ana and Biočanin, Vladimir and Milošević, Miloš and Trišić, Dijana and Lazović, Vladimir and Zogović, Nevena and Milašin, Jelena and Ilić, Dragan",
year = "2021",
abstract = "The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p < 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p < 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p < 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p < 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p < 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements’ surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.",
publisher = "Oxford: Elsevier Ltd",
journal = "Ceramics International",
title = "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic",
number = "20",
volume = "47",
doi = "10.1016/j.ceramint.2021.07.052",
pages = "28913-28923"
}

Antonijević, Đ., Despotović, A., Biočanin, V., Milošević, M., Trišić, D., Lazović, V., Zogović, N., Milašin, J.,& Ilić, D.. (2021). Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International
Oxford: Elsevier Ltd., 47(20), 28913-28923.
https://doi.org/10.1016/j.ceramint.2021.07.052

Antonijević Đ, Despotović A, Biočanin V, Milošević M, Trišić D, Lazović V, Zogović N, Milašin J, Ilić D. Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International. 2021;47(20):28913-28923.
doi:10.1016/j.ceramint.2021.07.052 .

Antonijević, Đorđe, Despotović, Ana, Biočanin, Vladimir, Milošević, Miloš, Trišić, Dijana, Lazović, Vladimir, Zogović, Nevena, Milašin, Jelena, Ilić, Dragan, "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic" in Ceramics International, 47, no. 20 (2021):28913-28923,
https://doi.org/10.1016/j.ceramint.2021.07.052 . .

TY  - JOUR
AU  - Vodnik, Vesna V.
AU  - Mojić, Marija
AU  - Stamenović, Una
AU  - Otoničar, Mojca
AU  - Ajdžanović, Vladimir
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Marković, Mirjana M.
AU  - Barudžija, Tanja
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4238
AB  - Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells.
PB  - Elsevier Ltd
T2  - Materials Science and Engineering C
T1  - Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines
VL  - 124
DO  - 10.1016/j.msec.2021.112078
SP  - 112078
ER  - 

@article{
author = "Vodnik, Vesna V. and Mojić, Marija and Stamenović, Una and Otoničar, Mojca and Ajdžanović, Vladimir and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Marković, Mirjana M. and Barudžija, Tanja and Filipović, Branko and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2021",
abstract = "Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells.",
publisher = "Elsevier Ltd",
journal = "Materials Science and Engineering C",
title = "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines",
volume = "124",
doi = "10.1016/j.msec.2021.112078",
pages = "112078"
}

Vodnik, V. V., Mojić, M., Stamenović, U., Otoničar, M., Ajdžanović, V., Maksimović-Ivanić, D., Mijatović, S., Marković, M. M., Barudžija, T., Filipović, B., Milošević, V.,& Šošić-Jurjević, B.. (2021). Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering C
Elsevier Ltd., 124, 112078.
https://doi.org/10.1016/j.msec.2021.112078

Vodnik VV, Mojić M, Stamenović U, Otoničar M, Ajdžanović V, Maksimović-Ivanić D, Mijatović S, Marković MM, Barudžija T, Filipović B, Milošević V, Šošić-Jurjević B. Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering C. 2021;124:112078.
doi:10.1016/j.msec.2021.112078 .

Vodnik, Vesna V., Mojić, Marija, Stamenović, Una, Otoničar, Mojca, Ajdžanović, Vladimir, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Marković, Mirjana M., Barudžija, Tanja, Filipović, Branko, Milošević, Verica, Šošić-Jurjević, Branka , "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines" in Materials Science and Engineering C, 124 (2021):112078,
https://doi.org/10.1016/j.msec.2021.112078 . .