TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana
AU  - Milinčić, Danijel
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6687
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
VL  - 17
DO  - 10.3390/ph17040518
SP  - 518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana and Milinčić, Danijel and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
volume = "17",
doi = "10.3390/ph17040518",
pages = "518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M., Milinčić, D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
Basel: MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić M, Milinčić D, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana, Milinčić, Danijel, Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Kasalović, Marijana P.
AU  - Dimić, Dušan
AU  - Jelača, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Zmejkovski, Bojana B.
AU  - Schreiner, Simon H. F.
AU  - Rüffer, Tobias
AU  - Pantelić, Nebojša Đ.
AU  - Kaluđerović, Goran N.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6626
AB  - A novel trimethyltin(IV) complex (Me3SnL), derived from 3-(4-methyl-2-oxoquinolin-1(2H)-yl)propanoate ligand, has been synthesized and characterized by elemental microanalysis, UV/Vis spectrophotometry, FT-IR and multinuclear (1H, 13C and 119Sn) NMR spectroscopies. Furthermore, the structure of the ligand precursor HL was solved using SC-XRD (single-crystal X-ray diffraction). The prediction of UV/Vis and NMR spectra by quantum-chemical methods was performed and compared to experimental findings. The protein binding affinity of Me3SnL towards BSA was determined by spectrofluorometric titration and subsequent molecular docking simulations. Me3SnL has been evaluated for its in vitro anticancer activity against three human cell lines, MCF-7 (breast adenocarcinoma), A375 (melanoma) and HCT116 (colorectal carcinoma), and three mouse tumor cell lines, 4T1 (breast carcinoma), B16 (melanoma) and CT26 (colon carcinoma), using MTT and CV assays. The strong inhibition of A375 cell proliferation, ROS/RNS upregulation and robust lipid peroxidation lead to autophagic cell death upon treatment with Me3SnL.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Trimethyltin(IV) Bearing 3-(4-Methyl-2-oxoquinolin-1(2H)-yl)propanoate Causes Lipid Peroxidation-Mediated Autophagic Cell Death in Human Melanoma A375 Cells
IS  - 3
VL  - 17
DO  - 10.3390/ph17030372
SP  - 372
ER  - 

@article{
author = "Kasalović, Marijana P. and Dimić, Dušan and Jelača, Sanja and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Zmejkovski, Bojana B. and Schreiner, Simon H. F. and Rüffer, Tobias and Pantelić, Nebojša Đ. and Kaluđerović, Goran N.",
year = "2024",
abstract = "A novel trimethyltin(IV) complex (Me3SnL), derived from 3-(4-methyl-2-oxoquinolin-1(2H)-yl)propanoate ligand, has been synthesized and characterized by elemental microanalysis, UV/Vis spectrophotometry, FT-IR and multinuclear (1H, 13C and 119Sn) NMR spectroscopies. Furthermore, the structure of the ligand precursor HL was solved using SC-XRD (single-crystal X-ray diffraction). The prediction of UV/Vis and NMR spectra by quantum-chemical methods was performed and compared to experimental findings. The protein binding affinity of Me3SnL towards BSA was determined by spectrofluorometric titration and subsequent molecular docking simulations. Me3SnL has been evaluated for its in vitro anticancer activity against three human cell lines, MCF-7 (breast adenocarcinoma), A375 (melanoma) and HCT116 (colorectal carcinoma), and three mouse tumor cell lines, 4T1 (breast carcinoma), B16 (melanoma) and CT26 (colon carcinoma), using MTT and CV assays. The strong inhibition of A375 cell proliferation, ROS/RNS upregulation and robust lipid peroxidation lead to autophagic cell death upon treatment with Me3SnL.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Trimethyltin(IV) Bearing 3-(4-Methyl-2-oxoquinolin-1(2H)-yl)propanoate Causes Lipid Peroxidation-Mediated Autophagic Cell Death in Human Melanoma A375 Cells",
number = "3",
volume = "17",
doi = "10.3390/ph17030372",
pages = "372"
}

Kasalović, M. P., Dimić, D., Jelača, S., Maksimović-Ivanić, D., Mijatović, S., Zmejkovski, B. B., Schreiner, S. H. F., Rüffer, T., Pantelić, N. Đ.,& Kaluđerović, G. N.. (2024). Trimethyltin(IV) Bearing 3-(4-Methyl-2-oxoquinolin-1(2H)-yl)propanoate Causes Lipid Peroxidation-Mediated Autophagic Cell Death in Human Melanoma A375 Cells. in Pharmaceuticals
Basel: MDPI., 17(3), 372.
https://doi.org/10.3390/ph17030372

Kasalović MP, Dimić D, Jelača S, Maksimović-Ivanić D, Mijatović S, Zmejkovski BB, Schreiner SHF, Rüffer T, Pantelić NĐ, Kaluđerović GN. Trimethyltin(IV) Bearing 3-(4-Methyl-2-oxoquinolin-1(2H)-yl)propanoate Causes Lipid Peroxidation-Mediated Autophagic Cell Death in Human Melanoma A375 Cells. in Pharmaceuticals. 2024;17(3):372.
doi:10.3390/ph17030372 .

Kasalović, Marijana P., Dimić, Dušan, Jelača, Sanja, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Zmejkovski, Bojana B., Schreiner, Simon H. F., Rüffer, Tobias, Pantelić, Nebojša Đ., Kaluđerović, Goran N., "Trimethyltin(IV) Bearing 3-(4-Methyl-2-oxoquinolin-1(2H)-yl)propanoate Causes Lipid Peroxidation-Mediated Autophagic Cell Death in Human Melanoma A375 Cells" in Pharmaceuticals, 17, no. 3 (2024):372,
https://doi.org/10.3390/ph17030372 . .

TY  - JOUR
AU  - Kasalović, Marijana P.
AU  - Jelača, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Lađarević, Jelena
AU  - Radovanović, Lidija
AU  - Božić, Bojan
AU  - Mijatović, Sanja
AU  - Pantelić, Nebojša Đ.
AU  - Kaluđerović, Goran N.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6268
AB  - Three new diphenyltin(IV) complexes, bis(3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoato)diphenyltin(IV) (1), bis(2-(4-methyl-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (2), and bis(2-(4-hydroxy-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (3), were synthesized and characterized by elemental microanalysis, FT-IR spectroscopy, and multinuclear (1H, 13C and 119Sn) NMR spectroscopy. Crystal structure of ligand precursor, 3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoic acid (HL1), has been determined by X-ray diffraction studies. Asymmetric bidentate coordination of the carboxylato ligands and skew trapezoidal structures are assumed for the synthesized complexes. In vitro anticancer activity of the synthesized diphenyltin(IV) complexes was evaluated against three human: MCF-7 (breast adenocarcinoma), A375 (melanoma), HCT116 (colorectal carcinoma), and three mouse tumor cell lines: 4 T1 (breast carcinoma), B16 (melanoma), CT26 (colon carcinoma) using MTT and CV assays. The IC50 values fall in the range from 0.1 to 3.7 μM. Flow cytometric analysis and fluorescent microscopy suggest that complex 1 induces caspase-dependent apoptosis followed with strong blockade of cell division in HCT116 cells. Since complex 1 showed ROS/RNS scavenging potential mentioned cytotoxicity was not connected with oxidative stress.
PB  - Elsevier
T2  - Journal of Inorganic Biochemistry
T1  - Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies
VL  - 250
DO  - 10.1016/j.jinorgbio.2023.112399
SP  - 112399
ER  - 

@article{
author = "Kasalović, Marijana P. and Jelača, Sanja and Maksimović-Ivanić, Danijela and Lađarević, Jelena and Radovanović, Lidija and Božić, Bojan and Mijatović, Sanja and Pantelić, Nebojša Đ. and Kaluđerović, Goran N.",
year = "2024",
abstract = "Three new diphenyltin(IV) complexes, bis(3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoato)diphenyltin(IV) (1), bis(2-(4-methyl-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (2), and bis(2-(4-hydroxy-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (3), were synthesized and characterized by elemental microanalysis, FT-IR spectroscopy, and multinuclear (1H, 13C and 119Sn) NMR spectroscopy. Crystal structure of ligand precursor, 3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoic acid (HL1), has been determined by X-ray diffraction studies. Asymmetric bidentate coordination of the carboxylato ligands and skew trapezoidal structures are assumed for the synthesized complexes. In vitro anticancer activity of the synthesized diphenyltin(IV) complexes was evaluated against three human: MCF-7 (breast adenocarcinoma), A375 (melanoma), HCT116 (colorectal carcinoma), and three mouse tumor cell lines: 4 T1 (breast carcinoma), B16 (melanoma), CT26 (colon carcinoma) using MTT and CV assays. The IC50 values fall in the range from 0.1 to 3.7 μM. Flow cytometric analysis and fluorescent microscopy suggest that complex 1 induces caspase-dependent apoptosis followed with strong blockade of cell division in HCT116 cells. Since complex 1 showed ROS/RNS scavenging potential mentioned cytotoxicity was not connected with oxidative stress.",
publisher = "Elsevier",
journal = "Journal of Inorganic Biochemistry",
title = "Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies",
volume = "250",
doi = "10.1016/j.jinorgbio.2023.112399",
pages = "112399"
}

Kasalović, M. P., Jelača, S., Maksimović-Ivanić, D., Lađarević, J., Radovanović, L., Božić, B., Mijatović, S., Pantelić, N. Đ.,& Kaluđerović, G. N.. (2024). Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies. in Journal of Inorganic Biochemistry
Elsevier., 250, 112399.
https://doi.org/10.1016/j.jinorgbio.2023.112399

Kasalović MP, Jelača S, Maksimović-Ivanić D, Lađarević J, Radovanović L, Božić B, Mijatović S, Pantelić NĐ, Kaluđerović GN. Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies. in Journal of Inorganic Biochemistry. 2024;250:112399.
doi:10.1016/j.jinorgbio.2023.112399 .

Kasalović, Marijana P., Jelača, Sanja, Maksimović-Ivanić, Danijela, Lađarević, Jelena, Radovanović, Lidija, Božić, Bojan, Mijatović, Sanja, Pantelić, Nebojša Đ., Kaluđerović, Goran N., "Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies" in Journal of Inorganic Biochemistry, 250 (2024):112399,
https://doi.org/10.1016/j.jinorgbio.2023.112399 . .

TY  - JOUR
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Kolašinac, Stefan
AU  - Rac, Vladislav
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Banjac, Nebojša
AU  - Lađarević, Jelena
AU  - Vidović, Bojana
AU  - Kostić, Aleksandar
AU  - Pavlović, Vladimir
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5140
AB  - This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.
PB  - Oxford: Elsevier
T2  - Food Chemistry
T1  - Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization
VL  - 402
DO  - 10.1016/j.foodchem.2022.134299
SP  - 134299
ER  - 

@article{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Kolašinac, Stefan and Rac, Vladislav and Petrović, Jovana and Soković, Marina and Banjac, Nebojša and Lađarević, Jelena and Vidović, Bojana and Kostić, Aleksandar and Pavlović, Vladimir and Pešić, Mirjana",
year = "2023",
abstract = "This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.",
publisher = "Oxford: Elsevier",
journal = "Food Chemistry",
title = "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization",
volume = "402",
doi = "10.1016/j.foodchem.2022.134299",
pages = "134299"
}

Popović Minić, D., Milinčić, D., Kolašinac, S., Rac, V., Petrović, J., Soković, M., Banjac, N., Lađarević, J., Vidović, B., Kostić, A., Pavlović, V.,& Pešić, M.. (2023). Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry
Oxford: Elsevier., 402, 134299.
https://doi.org/10.1016/j.foodchem.2022.134299

Popović Minić D, Milinčić D, Kolašinac S, Rac V, Petrović J, Soković M, Banjac N, Lađarević J, Vidović B, Kostić A, Pavlović V, Pešić M. Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry. 2023;402:134299.
doi:10.1016/j.foodchem.2022.134299 .

Popović Minić, Dušanka, Milinčić, Danijel, Kolašinac, Stefan, Rac, Vladislav, Petrović, Jovana, Soković, Marina, Banjac, Nebojša, Lađarević, Jelena, Vidović, Bojana, Kostić, Aleksandar, Pavlović, Vladimir, Pešić, Mirjana, "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization" in Food Chemistry, 402 (2023):134299,
https://doi.org/10.1016/j.foodchem.2022.134299 . .

TY  - CONF
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Poštić, Dobrivoj
AU  - Momčilović, Ivana
PY  - 2023
UR  - https://agrif.bg.ac.rs/uploads/files/strane/Fakultet/Izdavacka_delatnost/Zbornici_radova/XI%20Simpozijum%20sa%20medjunarodnim%20ucescem%20-%20Inovacije%20u%20ratarskoj%20i%20povrtarskoj%20proizvodnji.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6487
AB  - Aeroponics is modern, soilless technology for potato cultivation that promotes the production
of a large number of healthy minitubers (pre-basic seed potatoes). In aeroponics, roots and underground
stems (stolons) of potato plants grow inside closed boxes (modules), suspended in the fine mist of a
nutrient solution that recirculates through the system. As a result, a significant number of tubers larger
than 1 cm can be formed on stolons during the growing period. The aim of this study was to evaluate
the effects of the genotype and origin of planting material on the production of minituber in an aeroponic
facility in Guča, Serbia. Our study encompassed three potato cultivars: Cleopatra, Kennebec and
Désirée, and two types of planting material: acclimated microplants and plants originated from the
previous season’s minitubers. Plants were aeroponically cultivated in the 2019 season (late May-
December) with a planting density of 24 plants per m2 and ~14-day harvest intervals. The largest
number of minitubers per plant (19.9) was recorded for cultivar Désirée, followed by the Kennebec
(15.7) and Cleopatra (11.1). The average mass of minitubers of all the cultivars under consideration
was significantly higher in the plants originating from minitubers than in plants of in vitro origin. The
highest yield of minitubers, 10.27 kg m-2, was recorded for Kennebec plants of minituber origin.
Besides, the plants originated from minitubers steadily tuberized during the entire cultivation period
in the aeroponic facility.
AB  - Аеропоника је савремена технологија гајења биљака без земље која омогућава ефикасну
производњу здравог предосновног семенског кромпира у форми миникртола. У аеропонском
систему, коренови и столони биљака кромпира расту унутар затворених боксова (модула) у
магли капљица хранљивог раствора који циркулише кроз систем. Као резултат оваквог гајења,
значајан број кртола већих од 1 cm може се формирати на столонима биљака током вегетационог
периода. Циљ овог истраживања био је да се процене ефекти генотипа кромпира и порекла
садног материјала на производњу миникртола у објекту са аеропоником у Гучи, Србија. Наша
студија је обухватила три сорте кромпира: Cleopatra, Kennebec и Désirée, и две врсте садног
материјала: аклиматизоване микробиљке и биљке добијене из миникртола (род из претходне
сезоне). Биљке су узгајане аеропонски у сезони 2019. (крај маја-децембар) са густином садње
од 24 биљке по m2 и интервалима жетве од ~14 дана. Највећи број миникртола по биљци (19,9)
формирала је сорта Désirée, затим Kennebec (15,7) и Cleopatra (11,1). Просечна маса миникртола
свих испитиваних сорти била је значајно већа код биљака добијених из миникртола него код
биљака in vitro порекла. Највећи принос миникртола, 10,27 kg m-2, установљен је код биљака
сорте Kennebec узгојених из миникртола. Поред тога, биљке пореклом из миникртола су постојано
формирале кртоле током читавог периода гајења у аеропонском систему.
PB  - Belgrade: University of Belgrade, Faculty of Agriculture
C3  - Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia
T1  - Potato minituber production by aeroponics: effects of plant origin and genotype
T1  - Аеропонска производња миникртола кромпира: утицај порекла садног материјала и генотипа
SP  - 31
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6487
ER  - 

@conference{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Poštić, Dobrivoj and Momčilović, Ivana",
year = "2023",
abstract = "Aeroponics is modern, soilless technology for potato cultivation that promotes the production
of a large number of healthy minitubers (pre-basic seed potatoes). In aeroponics, roots and underground
stems (stolons) of potato plants grow inside closed boxes (modules), suspended in the fine mist of a
nutrient solution that recirculates through the system. As a result, a significant number of tubers larger
than 1 cm can be formed on stolons during the growing period. The aim of this study was to evaluate
the effects of the genotype and origin of planting material on the production of minituber in an aeroponic
facility in Guča, Serbia. Our study encompassed three potato cultivars: Cleopatra, Kennebec and
Désirée, and two types of planting material: acclimated microplants and plants originated from the
previous season’s minitubers. Plants were aeroponically cultivated in the 2019 season (late May-
December) with a planting density of 24 plants per m2 and ~14-day harvest intervals. The largest
number of minitubers per plant (19.9) was recorded for cultivar Désirée, followed by the Kennebec
(15.7) and Cleopatra (11.1). The average mass of minitubers of all the cultivars under consideration
was significantly higher in the plants originating from minitubers than in plants of in vitro origin. The
highest yield of minitubers, 10.27 kg m-2, was recorded for Kennebec plants of minituber origin.
Besides, the plants originated from minitubers steadily tuberized during the entire cultivation period
in the aeroponic facility., Аеропоника је савремена технологија гајења биљака без земље која омогућава ефикасну
производњу здравог предосновног семенског кромпира у форми миникртола. У аеропонском
систему, коренови и столони биљака кромпира расту унутар затворених боксова (модула) у
магли капљица хранљивог раствора који циркулише кроз систем. Као резултат оваквог гајења,
значајан број кртола већих од 1 cm може се формирати на столонима биљака током вегетационог
периода. Циљ овог истраживања био је да се процене ефекти генотипа кромпира и порекла
садног материјала на производњу миникртола у објекту са аеропоником у Гучи, Србија. Наша
студија је обухватила три сорте кромпира: Cleopatra, Kennebec и Désirée, и две врсте садног
материјала: аклиматизоване микробиљке и биљке добијене из миникртола (род из претходне
сезоне). Биљке су узгајане аеропонски у сезони 2019. (крај маја-децембар) са густином садње
од 24 биљке по m2 и интервалима жетве од ~14 дана. Највећи број миникртола по биљци (19,9)
формирала је сорта Désirée, затим Kennebec (15,7) и Cleopatra (11,1). Просечна маса миникртола
свих испитиваних сорти била је значајно већа код биљака добијених из миникртола него код
биљака in vitro порекла. Највећи принос миникртола, 10,27 kg m-2, установљен је код биљака
сорте Kennebec узгојених из миникртола. Поред тога, биљке пореклом из миникртола су постојано
формирале кртоле током читавог периода гајења у аеропонском систему.",
publisher = "Belgrade: University of Belgrade, Faculty of Agriculture",
journal = "Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia",
title = "Potato minituber production by aeroponics: effects of plant origin and genotype, Аеропонска производња миникртола кромпира: утицај порекла садног материјала и генотипа",
pages = "31-32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6487"
}

Broćić, Z., Oljača, J., Pantelić, D., Rudić, J., Poštić, D.,& Momčilović, I.. (2023). Potato minituber production by aeroponics: effects of plant origin and genotype. in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Agriculture., 31-32.
https://hdl.handle.net/21.15107/rcub_ibiss_6487

Broćić Z, Oljača J, Pantelić D, Rudić J, Poštić D, Momčilović I. Potato minituber production by aeroponics: effects of plant origin and genotype. in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia. 2023;:31-32.
https://hdl.handle.net/21.15107/rcub_ibiss_6487 .

Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Poštić, Dobrivoj, Momčilović, Ivana, "Potato minituber production by aeroponics: effects of plant origin and genotype" in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia (2023):31-32,
https://hdl.handle.net/21.15107/rcub_ibiss_6487 .

TY  - CONF
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Poštić, Dobrivoj
AU  - Momčilović, Ivana
PY  - 2023
UR  - https://agrores.agro.unibl.org/wp-content/uploads/2023/05/Book-of-Proceedings-AgroReS-2023.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6488
AB  - Aeroponics is eco-friendly, soilless technology for the cultivation of vegetable plants that can
be used for the production of pathogen-free pre-basic seed potato, namely minitubers. In
aeroponic modules, the underground parts of potato plants grow suspended in the mist of finely
dispersed nutrient solution to produce tubers, while the shoots grow above the module under
greenhouse conditions. This study aimed to evaluate the effects of the cultivar and origin of planting material on the minituber production in an aeroponic faility in Guča, Serbia. Two potato cultivars, Sinora and Agria, and two types of planting material, acclimated microplants
and plants originating from sprouted minitubers, were used in the study. Plants were grown in
the 2019 season with a planting density of 24 plants per m2 and ~14-day harvest intervals.
Agria plants of both plant origins steadily tuberized during most of the cultivation period and
formed a significantly larger number of minitubers (13.61) compared to cultivar Sinora (3.35),
which quickly completed the growth cycle. In both investigated cultivars, the mass of formed
minitubers was significantly higher in the plants originating from minitubers (18.80 g)
compared to plants of in vitro origin (9.04 g). Agria plants of minituber origin produced the
heaviest minitubers (19.05 g), while Sinora plants of in vitro origin formed the least heavy
tubers (5.29 g). The highest yield of minitubers, 6.26 kg m-2, was recorded for Agria plants of
minituber origin. The results of our study suggest that both plant origin and potato genotype
significantly affect minituber production in aeroponics.
PB  - Banja Luka: University of Banja Luka, Faculty of Agriculture
C3  - Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina
T1  - Effects of cultivar and plant origin on the aeroponic production of potato minitubers
DO  - 10.7251/ZARS2301009B
SP  - 9
EP  - 18
ER  - 

@conference{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Poštić, Dobrivoj and Momčilović, Ivana",
year = "2023",
abstract = "Aeroponics is eco-friendly, soilless technology for the cultivation of vegetable plants that can
be used for the production of pathogen-free pre-basic seed potato, namely minitubers. In
aeroponic modules, the underground parts of potato plants grow suspended in the mist of finely
dispersed nutrient solution to produce tubers, while the shoots grow above the module under
greenhouse conditions. This study aimed to evaluate the effects of the cultivar and origin of planting material on the minituber production in an aeroponic faility in Guča, Serbia. Two potato cultivars, Sinora and Agria, and two types of planting material, acclimated microplants
and plants originating from sprouted minitubers, were used in the study. Plants were grown in
the 2019 season with a planting density of 24 plants per m2 and ~14-day harvest intervals.
Agria plants of both plant origins steadily tuberized during most of the cultivation period and
formed a significantly larger number of minitubers (13.61) compared to cultivar Sinora (3.35),
which quickly completed the growth cycle. In both investigated cultivars, the mass of formed
minitubers was significantly higher in the plants originating from minitubers (18.80 g)
compared to plants of in vitro origin (9.04 g). Agria plants of minituber origin produced the
heaviest minitubers (19.05 g), while Sinora plants of in vitro origin formed the least heavy
tubers (5.29 g). The highest yield of minitubers, 6.26 kg m-2, was recorded for Agria plants of
minituber origin. The results of our study suggest that both plant origin and potato genotype
significantly affect minituber production in aeroponics.",
publisher = "Banja Luka: University of Banja Luka, Faculty of Agriculture",
journal = "Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina",
title = "Effects of cultivar and plant origin on the aeroponic production of potato minitubers",
doi = "10.7251/ZARS2301009B",
pages = "9-18"
}

Broćić, Z., Oljača, J., Pantelić, D., Rudić, J., Poštić, D.,& Momčilović, I.. (2023). Effects of cultivar and plant origin on the aeroponic production of potato minitubers. in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina
Banja Luka: University of Banja Luka, Faculty of Agriculture., 9-18.
https://doi.org/10.7251/ZARS2301009B

Broćić Z, Oljača J, Pantelić D, Rudić J, Poštić D, Momčilović I. Effects of cultivar and plant origin on the aeroponic production of potato minitubers. in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina. 2023;:9-18.
doi:10.7251/ZARS2301009B .

Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Poštić, Dobrivoj, Momčilović, Ivana, "Effects of cultivar and plant origin on the aeroponic production of potato minitubers" in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina (2023):9-18,
https://doi.org/10.7251/ZARS2301009B . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Špirović Trifunović, Bojana
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Stanojević, Sladjana
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6466
AB  - The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties
IS  - 7
VL  - 12
DO  - 10.3390/antiox12071424
SP  - 1424
ER  - 

@article{
author = "Kostić, Aleksandar and Milinčić, Danijel and Špirović Trifunović, Bojana and Nedić, Nebojša and Gašić, Uroš and Tešić, Živoslav and Stanojević, Sladjana and Pešić, Mirjana",
year = "2023",
abstract = "The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties",
number = "7",
volume = "12",
doi = "10.3390/antiox12071424",
pages = "1424"
}

Kostić, A., Milinčić, D., Špirović Trifunović, B., Nedić, N., Gašić, U., Tešić, Ž., Stanojević, S.,& Pešić, M.. (2023). Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants
Basel: MDPI., 12(7), 1424.
https://doi.org/10.3390/antiox12071424

Kostić A, Milinčić D, Špirović Trifunović B, Nedić N, Gašić U, Tešić Ž, Stanojević S, Pešić M. Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants. 2023;12(7):1424.
doi:10.3390/antiox12071424 .

Kostić, Aleksandar, Milinčić, Danijel, Špirović Trifunović, Bojana, Nedić, Nebojša, Gašić, Uroš, Tešić, Živoslav, Stanojević, Sladjana, Pešić, Mirjana, "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties" in Antioxidants, 12, no. 7 (2023):1424,
https://doi.org/10.3390/antiox12071424 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Tešić, Živoslav
AU  - Kalaba, Milica
AU  - Ćirić, Ivanka
AU  - Pezo, Lato
AU  - Lončar, Biljana
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Tosti, Tomislav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6467
AB  - The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040477
SP  - 477
ER  - 

@article{
author = "Fotirić Akšić, Milica and Tešić, Živoslav and Kalaba, Milica and Ćirić, Ivanka and Pezo, Lato and Lončar, Biljana and Gašić, Uroš and Dojčinović, Biljana and Tosti, Tomislav and Meland, Mekjell",
year = "2023",
abstract = "The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040477",
pages = "477"
}

Fotirić Akšić, M., Tešić, Ž., Kalaba, M., Ćirić, I., Pezo, L., Lončar, B., Gašić, U., Dojčinović, B., Tosti, T.,& Meland, M.. (2023). Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae
Basel: MDPI., 9(4), 477.
https://doi.org/10.3390/horticulturae9040477

Fotirić Akšić M, Tešić Ž, Kalaba M, Ćirić I, Pezo L, Lončar B, Gašić U, Dojčinović B, Tosti T, Meland M. Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae. 2023;9(4):477.
doi:10.3390/horticulturae9040477 .

Fotirić Akšić, Milica, Tešić, Živoslav, Kalaba, Milica, Ćirić, Ivanka, Pezo, Lato, Lončar, Biljana, Gašić, Uroš, Dojčinović, Biljana, Tosti, Tomislav, Meland, Mekjell, "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway" in Horticulturae, 9, no. 4 (2023):477,
https://doi.org/10.3390/horticulturae9040477 . .

TY  - CONF
AU  - Pajić, Tanja
AU  - Stevanović, Katarina
AU  - Todorović, Nataša
AU  - Lević, Steva
AU  - Savić Šević, Svetlana
AU  - Pantelić, Dejan
AU  - Živić, Miroslav
AU  - Rabasović, Mihailo D
AU  - Krmpot, Aleksandar J
PY  - 2023
UR  - https://www.eventclass.org/contxt_emim2023/online-program/session?s=PW36#e609
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6289
AB  - Introduction
Electrophysiology studies of ion channels, in live filamentous fungi by patch clamp method are notpossible due to presence of rigid chitinous cell wall that prevents patch clamp pipette to access theplasma membrane. We present laser nano-surgery of the fungal cell wall that enables patch clampelectrophysiology studies. Similar approaches as one-time reports utilizing nanosecond laser pulseslong time ago were not pursued further [1,2]. Here, we demonstrate reproducible method usingfemtosecond lasers accompanied by two-photon excitation fluorescence (TPEF) imaging of hyphae.
Methods
A wild-type strain of filamentous fungus
Phycomyces blakesleeanus
(Burgeff ) [NRRL 1555(-)] were grownon glass coverslips with hand-etched grid, coated with a thin layer of 50% collagen type I as animmobilizer. Home built nonlinear laser scanning microscope [3,4] utilizing Ti:Sa tunable fs laser wasused for TPEF imaging of hyphae and the cell surgery. The latter is enabled with the custom made add-on in soft ware. Coverslip with hyphae is transferred to another microscope setup for patch clamp,consisting of micromanipulators and precise electronics for pA current measurements. The surgicalincisions and released protoplasts were additionally imaged by scanning electron microscopy for whichtreated hyphae had to undergo critical point drying procedure.
Results/Discussion
Hyphae were stained by Calcofluor White and treated with an exocytosis inhibitor (brefeldin A) and arespiration inhibitor (sodium azide) to prevent cell wall regeneration. Since the cell wall and the plasmamembrane are in the close contact [4] hyphae were kept in hyperosmotic solution to retract thecytoplasm from the cell wall. Surgical spot-wise pattern was precisely positioned at TPEF image ofselected hypha at the place where the plasma membrane was retracted. The dwell time (1s) and thelaser power (4-15mW) were set with fixed repetition rate (76MHz), pulse duration (160fs) and laserwavelength (730nm). Upon the surgery, hyphae were gently deplasmolysed. A protoplast with plasmamembrane accessible for the patch clamp pipette was released through the surgical incision (Fig 1). The>GΩ seal resistance was achieved. Numerous ion channels are recorded in diff erent configurations (oncell, inside-out, whole cell and out-out) (Fig 2).
Conclusions
The whole process (cell surgery + patch clamping) is rather complex and specific steps have to be strictlyfollowed for high success rate and reproducibility. Also, chemicals concentrations, solutions osmolarity,timing and cutting parameters have to be kept in the specified narrow range. Obtained currentrecordings provide valuable information on fungal cell membrane ionic channels.
PB  - European Society for Molecular Imaging
C3  - European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria
T1  - Laser nano-surgery of fungal cell wall to enable patch clamping
SP  - 1095
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6289
ER  - 

@conference{
author = "Pajić, Tanja and Stevanović, Katarina and Todorović, Nataša and Lević, Steva and Savić Šević, Svetlana and Pantelić, Dejan and Živić, Miroslav and Rabasović, Mihailo D and Krmpot, Aleksandar J",
year = "2023",
abstract = "Introduction
Electrophysiology studies of ion channels, in live filamentous fungi by patch clamp method are notpossible due to presence of rigid chitinous cell wall that prevents patch clamp pipette to access theplasma membrane. We present laser nano-surgery of the fungal cell wall that enables patch clampelectrophysiology studies. Similar approaches as one-time reports utilizing nanosecond laser pulseslong time ago were not pursued further [1,2]. Here, we demonstrate reproducible method usingfemtosecond lasers accompanied by two-photon excitation fluorescence (TPEF) imaging of hyphae.
Methods
A wild-type strain of filamentous fungus
Phycomyces blakesleeanus
(Burgeff ) [NRRL 1555(-)] were grownon glass coverslips with hand-etched grid, coated with a thin layer of 50% collagen type I as animmobilizer. Home built nonlinear laser scanning microscope [3,4] utilizing Ti:Sa tunable fs laser wasused for TPEF imaging of hyphae and the cell surgery. The latter is enabled with the custom made add-on in soft ware. Coverslip with hyphae is transferred to another microscope setup for patch clamp,consisting of micromanipulators and precise electronics for pA current measurements. The surgicalincisions and released protoplasts were additionally imaged by scanning electron microscopy for whichtreated hyphae had to undergo critical point drying procedure.
Results/Discussion
Hyphae were stained by Calcofluor White and treated with an exocytosis inhibitor (brefeldin A) and arespiration inhibitor (sodium azide) to prevent cell wall regeneration. Since the cell wall and the plasmamembrane are in the close contact [4] hyphae were kept in hyperosmotic solution to retract thecytoplasm from the cell wall. Surgical spot-wise pattern was precisely positioned at TPEF image ofselected hypha at the place where the plasma membrane was retracted. The dwell time (1s) and thelaser power (4-15mW) were set with fixed repetition rate (76MHz), pulse duration (160fs) and laserwavelength (730nm). Upon the surgery, hyphae were gently deplasmolysed. A protoplast with plasmamembrane accessible for the patch clamp pipette was released through the surgical incision (Fig 1). The>GΩ seal resistance was achieved. Numerous ion channels are recorded in diff erent configurations (oncell, inside-out, whole cell and out-out) (Fig 2).
Conclusions
The whole process (cell surgery + patch clamping) is rather complex and specific steps have to be strictlyfollowed for high success rate and reproducibility. Also, chemicals concentrations, solutions osmolarity,timing and cutting parameters have to be kept in the specified narrow range. Obtained currentrecordings provide valuable information on fungal cell membrane ionic channels.",
publisher = "European Society for Molecular Imaging",
journal = "European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria",
title = "Laser nano-surgery of fungal cell wall to enable patch clamping",
pages = "1095",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6289"
}

Pajić, T., Stevanović, K., Todorović, N., Lević, S., Savić Šević, S., Pantelić, D., Živić, M., Rabasović, M. D.,& Krmpot, A. J.. (2023). Laser nano-surgery of fungal cell wall to enable patch clamping. in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria
European Society for Molecular Imaging., 1095.
https://hdl.handle.net/21.15107/rcub_ibiss_6289

Pajić T, Stevanović K, Todorović N, Lević S, Savić Šević S, Pantelić D, Živić M, Rabasović MD, Krmpot AJ. Laser nano-surgery of fungal cell wall to enable patch clamping. in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria. 2023;:1095.
https://hdl.handle.net/21.15107/rcub_ibiss_6289 .

Pajić, Tanja, Stevanović, Katarina, Todorović, Nataša, Lević, Steva, Savić Šević, Svetlana, Pantelić, Dejan, Živić, Miroslav, Rabasović, Mihailo D, Krmpot, Aleksandar J, "Laser nano-surgery of fungal cell wall to enable patch clamping" in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria (2023):1095,
https://hdl.handle.net/21.15107/rcub_ibiss_6289 .

TY  - CONF
AU  - Jovanović, Mirna
AU  - Milosavljević, Dragica
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Milivojević, Jasminka
AU  - Đorđević, Ana
AU  - Brkljačić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6238
AB  - Insulin resistance is a state where a normal amount of insulin can’t provoke an appropriate metabolic response. Insulin promotes membrane trafficking of the glucose transporter GLUT4 from the storage vesicles to the plasma membrane in white adipose tissue. Adipocytes use glucose for lipogenesis and store the energy as lipid droplets. If adipocytes are unable to uptake glucose, a chronic state of hyperglycemia is developed, with severe health consequences. Polyphenols are natural anti-inflammatory and antioxidant agents. Food rich in polyphenols has been suggested to exert an ameliorative effect on restoring insulin sensitivity, with the main identified target being AMPK1,2, one of the key sensors of intracellular energy. Here, we tested the effect of methanol extracts from three newly introduced strawberry (Fragaria x ananassa, Duch.) cultivars – 'Aprika', 'Sandra' and 'Quicky' on glucose metabolism in 3T3-F442A adipocytes. It was determined that 'Aprika' has the highest total phenolic content, relative to the other two cultivars. After 72-h exposure, none of the strawberry cultivars affected adipocyte cell growth significantly. Protein expression analysis of the differentiated adipocytes suggested 'Aprika', but not the other two cultivars, significantly increased the AMPK expression, as well as GLUT4, thus increasing glucose uptake. Strawberry extracts did not significantly affect the differentiation of adipocytes (SIRT1 and PPARγ), nor the fatty acid synthesis (ACC). Conclusively, the 'Aprika' methanol extract with high phenolic content exerts an ameliorative effect on glucose uptake, presumably through activation of the AMPK-dependent mechanism of GLUT4 trafficking. The systemic effects of the ‘Aprika’ cultivar should be further investigated. Implications of the research are decreased hyperglycemia in obese and diabetic patients, by the introduction of the 'Aprika' strawberry cultivar into everyday diet. References 1. Zhao L, Zou T, Gomez NA et al. Raspberry alleviates obesity-induced inflammation and insulin resistance in skeletal muscle through activation of AMP-activated protein kinase (AMPK) α1. Nutr & Diabetes, 2018, 8, 39. 2. Xu W, Luo Y, Yin J, Luo F. Targeting AMPK signaling by polyphenols: A novel strategy for tackling aging. Food & Function. 2023.
PB  - Belgrade: Faculty of Chemistry
C3  - Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
T1  - Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes
SP  - 131
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6238
ER  - 

@conference{
author = "Jovanović, Mirna and Milosavljević, Dragica and Dragišić Maksimović, Jelena and Maksimović, Vuk and Milivojević, Jasminka and Đorđević, Ana and Brkljačić, Jelena",
year = "2023",
abstract = "Insulin resistance is a state where a normal amount of insulin can’t provoke an appropriate metabolic response. Insulin promotes membrane trafficking of the glucose transporter GLUT4 from the storage vesicles to the plasma membrane in white adipose tissue. Adipocytes use glucose for lipogenesis and store the energy as lipid droplets. If adipocytes are unable to uptake glucose, a chronic state of hyperglycemia is developed, with severe health consequences. Polyphenols are natural anti-inflammatory and antioxidant agents. Food rich in polyphenols has been suggested to exert an ameliorative effect on restoring insulin sensitivity, with the main identified target being AMPK1,2, one of the key sensors of intracellular energy. Here, we tested the effect of methanol extracts from three newly introduced strawberry (Fragaria x ananassa, Duch.) cultivars – 'Aprika', 'Sandra' and 'Quicky' on glucose metabolism in 3T3-F442A adipocytes. It was determined that 'Aprika' has the highest total phenolic content, relative to the other two cultivars. After 72-h exposure, none of the strawberry cultivars affected adipocyte cell growth significantly. Protein expression analysis of the differentiated adipocytes suggested 'Aprika', but not the other two cultivars, significantly increased the AMPK expression, as well as GLUT4, thus increasing glucose uptake. Strawberry extracts did not significantly affect the differentiation of adipocytes (SIRT1 and PPARγ), nor the fatty acid synthesis (ACC). Conclusively, the 'Aprika' methanol extract with high phenolic content exerts an ameliorative effect on glucose uptake, presumably through activation of the AMPK-dependent mechanism of GLUT4 trafficking. The systemic effects of the ‘Aprika’ cultivar should be further investigated. Implications of the research are decreased hyperglycemia in obese and diabetic patients, by the introduction of the 'Aprika' strawberry cultivar into everyday diet. References 1. Zhao L, Zou T, Gomez NA et al. Raspberry alleviates obesity-induced inflammation and insulin resistance in skeletal muscle through activation of AMP-activated protein kinase (AMPK) α1. Nutr & Diabetes, 2018, 8, 39. 2. Xu W, Luo Y, Yin J, Luo F. Targeting AMPK signaling by polyphenols: A novel strategy for tackling aging. Food & Function. 2023.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
title = "Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes",
pages = "131-132",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6238"
}

Jovanović, M., Milosavljević, D., Dragišić Maksimović, J., Maksimović, V., Milivojević, J., Đorđević, A.,& Brkljačić, J.. (2023). Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
Belgrade: Faculty of Chemistry., 131-132.
https://hdl.handle.net/21.15107/rcub_ibiss_6238

Jovanović M, Milosavljević D, Dragišić Maksimović J, Maksimović V, Milivojević J, Đorđević A, Brkljačić J. Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. 2023;:131-132.
https://hdl.handle.net/21.15107/rcub_ibiss_6238 .

Jovanović, Mirna, Milosavljević, Dragica, Dragišić Maksimović, Jelena, Maksimović, Vuk, Milivojević, Jasminka, Đorđević, Ana, Brkljačić, Jelena, "Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes" in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia (2023):131-132,
https://hdl.handle.net/21.15107/rcub_ibiss_6238 .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Poleksić, Vesna
AU  - Tasić, Aleksandra
AU  - Smederevac-Lalić, Marija
AU  - Đikanović, Vesna
AU  - Rašković, Božidar
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6095
AB  - Pikeperch of age classes 3+ and 4+ were collected from the Garaši reservoir (Serbia)
to analyze their bioindicator potential and compare their possible differences. Concentrations of
26 elements were determined in gills, muscles, and liver by inductively-coupled plasma optical
emission spectrometry (ICP-OES) and of 17 organochlorine pesticides (OCPs), and six polychlorinated
biphenyls (PCBs) in muscle by gas chromatography with mass spectrometric detection (GC-MS).
Histopathological changes in the liver and gills were analyzed as biomarkers of general fish health.
Only the concentrations of Cd, Na, and P in the muscles differed significantly. The OCPs and
PCBs concentrations were below the detection limits, so fish meat consumption does not pose a risk
concerning these substances. Hg and Cd exceeded the maximum allowed concentrations in some
4+ individuals, probably due to biomagnification. Gills were the most affected by metal exposure in
both age classes. Histopathological changes and indices were minor and did not differ significantly
between age classes, suggesting that pollution did not affect the morphology and structure of gills and
liver. There were no significant correlations between elemental accumulation and fish condition or
between histopathological scores. Therefore, both age classes can be used as bioindicators of pollution.
PB  - Basel: MDPI
T2  - Sustainability
T1  - Two Age Groups of Adult Pikeperch (Sander lucioperca) as Bioindicators of Aquatic Pollution
IS  - 14
VL  - 15
DO  - 10.3390/su151411321
SP  - 11321
ER  - 

@article{
author = "Nikolić, Dušan and Poleksić, Vesna and Tasić, Aleksandra and Smederevac-Lalić, Marija and Đikanović, Vesna and Rašković, Božidar",
year = "2023",
abstract = "Pikeperch of age classes 3+ and 4+ were collected from the Garaši reservoir (Serbia)
to analyze their bioindicator potential and compare their possible differences. Concentrations of
26 elements were determined in gills, muscles, and liver by inductively-coupled plasma optical
emission spectrometry (ICP-OES) and of 17 organochlorine pesticides (OCPs), and six polychlorinated
biphenyls (PCBs) in muscle by gas chromatography with mass spectrometric detection (GC-MS).
Histopathological changes in the liver and gills were analyzed as biomarkers of general fish health.
Only the concentrations of Cd, Na, and P in the muscles differed significantly. The OCPs and
PCBs concentrations were below the detection limits, so fish meat consumption does not pose a risk
concerning these substances. Hg and Cd exceeded the maximum allowed concentrations in some
4+ individuals, probably due to biomagnification. Gills were the most affected by metal exposure in
both age classes. Histopathological changes and indices were minor and did not differ significantly
between age classes, suggesting that pollution did not affect the morphology and structure of gills and
liver. There were no significant correlations between elemental accumulation and fish condition or
between histopathological scores. Therefore, both age classes can be used as bioindicators of pollution.",
publisher = "Basel: MDPI",
journal = "Sustainability",
title = "Two Age Groups of Adult Pikeperch (Sander lucioperca) as Bioindicators of Aquatic Pollution",
number = "14",
volume = "15",
doi = "10.3390/su151411321",
pages = "11321"
}

Nikolić, D., Poleksić, V., Tasić, A., Smederevac-Lalić, M., Đikanović, V.,& Rašković, B.. (2023). Two Age Groups of Adult Pikeperch (Sander lucioperca) as Bioindicators of Aquatic Pollution. in Sustainability
Basel: MDPI., 15(14), 11321.
https://doi.org/10.3390/su151411321

Nikolić D, Poleksić V, Tasić A, Smederevac-Lalić M, Đikanović V, Rašković B. Two Age Groups of Adult Pikeperch (Sander lucioperca) as Bioindicators of Aquatic Pollution. in Sustainability. 2023;15(14):11321.
doi:10.3390/su151411321 .

Nikolić, Dušan, Poleksić, Vesna, Tasić, Aleksandra, Smederevac-Lalić, Marija, Đikanović, Vesna, Rašković, Božidar, "Two Age Groups of Adult Pikeperch (Sander lucioperca) as Bioindicators of Aquatic Pollution" in Sustainability, 15, no. 14 (2023):11321,
https://doi.org/10.3390/su151411321 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5827
AB  - In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, and nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators of drought tolerance of I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, drought-stressed plants sprayed with distilled water, drought-stressed plants sprayed with 5 µM MeJA, and drought-stressed plants sprayed with 50 µM MeJA. Foliar spraying with MeJA was performed twice: seven days before and on the day of drought induction. The stressed plant groups were non-irrigated to achieve soil water contents (SWC) of 15 and 5%, while control plants were well-watered throughout the experiment (35–37% SWC). The results of this study showed that drought significantly reduced I. walleriana fresh and dry shoot weight, as well as total leaf area, but did not impact on dry matter content. The foliar application of MeJA improved growth parameters of I. walleriana, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC, and foliar applied MeJA at both concentrations. The flavonoid index was slightly reduced at 15 and 5% SWC when 50 µM MeJA was foliar applied, while there were no observed changes in the anthocyanin index in any treatments. The foliar application of 50 µM MeJA increased the chlorophyll index and NBI of I. walleriana at 5% SWC, indicating a contribution of the elicitor to plant drought tolerance at the physiological level.
PB  - Taylor & Francis Group
T2  - Plant Signaling & Behavior
T1  - Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress
IS  - 1
VL  - 18
DO  - 10.1080/15592324.2023.2219936
SP  - e2219936
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, and nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators of drought tolerance of I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, drought-stressed plants sprayed with distilled water, drought-stressed plants sprayed with 5 µM MeJA, and drought-stressed plants sprayed with 50 µM MeJA. Foliar spraying with MeJA was performed twice: seven days before and on the day of drought induction. The stressed plant groups were non-irrigated to achieve soil water contents (SWC) of 15 and 5%, while control plants were well-watered throughout the experiment (35–37% SWC). The results of this study showed that drought significantly reduced I. walleriana fresh and dry shoot weight, as well as total leaf area, but did not impact on dry matter content. The foliar application of MeJA improved growth parameters of I. walleriana, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC, and foliar applied MeJA at both concentrations. The flavonoid index was slightly reduced at 15 and 5% SWC when 50 µM MeJA was foliar applied, while there were no observed changes in the anthocyanin index in any treatments. The foliar application of 50 µM MeJA increased the chlorophyll index and NBI of I. walleriana at 5% SWC, indicating a contribution of the elicitor to plant drought tolerance at the physiological level.",
publisher = "Taylor & Francis Group",
journal = "Plant Signaling & Behavior",
title = "Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress",
number = "1",
volume = "18",
doi = "10.1080/15592324.2023.2219936",
pages = "e2219936"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress. in Plant Signaling & Behavior
Taylor & Francis Group., 18(1), e2219936.
https://doi.org/10.1080/15592324.2023.2219936

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress. in Plant Signaling & Behavior. 2023;18(1):e2219936.
doi:10.1080/15592324.2023.2219936 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress" in Plant Signaling & Behavior, 18, no. 1 (2023):e2219936,
https://doi.org/10.1080/15592324.2023.2219936 . .

TY  - JOUR
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar M.
AU  - Gašić, Uroš
AU  - Lazović, Mila
AU  - Fotirić Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka M.
AU  - Trifković, Jelena Đ.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5162
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity as one of the most important quality assurances, phenolic profiling by high-performance thin layer chromatography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry) revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.
PB  - Oxford: Elsevier Sci Ltd.
T2  - Food Chemistry
T1  - Authenticity assessment of cultivated berries via phenolic profiles of seeds
VL  - 402
DO  - 10.1016/j.foodchem.2022.134184
SP  - 134184
ER  - 

@article{
author = "Krstić, Đurđa D. and Ristivojević, Petar M. and Gašić, Uroš and Lazović, Mila and Fotirić Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka M. and Trifković, Jelena Đ.",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity as one of the most important quality assurances, phenolic profiling by high-performance thin layer chromatography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry) revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.",
publisher = "Oxford: Elsevier Sci Ltd.",
journal = "Food Chemistry",
title = "Authenticity assessment of cultivated berries via phenolic profiles of seeds",
volume = "402",
doi = "10.1016/j.foodchem.2022.134184",
pages = "134184"
}

Krstić, Đ. D., Ristivojević, P. M., Gašić, U., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry
Oxford: Elsevier Sci Ltd.., 402, 134184.
https://doi.org/10.1016/j.foodchem.2022.134184

Krstić ĐD, Ristivojević PM, Gašić U, Lazović M, Fotirić Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica DM, Trifković JĐ. Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry. 2023;402:134184.
doi:10.1016/j.foodchem.2022.134184 .

Krstić, Đurđa D., Ristivojević, Petar M., Gašić, Uroš, Lazović, Mila, Fotirić Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka M., Trifković, Jelena Đ., "Authenticity assessment of cultivated berries via phenolic profiles of seeds" in Food Chemistry, 402 (2023):134184,
https://doi.org/10.1016/j.foodchem.2022.134184 . .

TY  - JOUR
AU  - Milinčić, Danijel D
AU  - Stanisavljević, Nemanja S
AU  - Kostić, Aleksandar Ž
AU  - Gašić, Uroš
AU  - Stanojević, Slađana P
AU  - Tešić, Živoslav Lj
AU  - Pešić, Mirjana B
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/11/2164
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5236
AB  - This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein–phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion
IS  - 11
VL  - 11
DO  - 10.3390/antiox11112164
SP  - 2164
ER  - 

@article{
author = "Milinčić, Danijel D and Stanisavljević, Nemanja S and Kostić, Aleksandar Ž and Gašić, Uroš and Stanojević, Slađana P and Tešić, Živoslav Lj and Pešić, Mirjana B",
year = "2022",
abstract = "This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein–phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion",
number = "11",
volume = "11",
doi = "10.3390/antiox11112164",
pages = "2164"
}

Milinčić, D. D., Stanisavljević, N. S., Kostić, A. Ž., Gašić, U., Stanojević, S. P., Tešić, Ž. L.,& Pešić, M. B.. (2022). Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants
Basel: MDPI., 11(11), 2164.
https://doi.org/10.3390/antiox11112164

Milinčić DD, Stanisavljević NS, Kostić AŽ, Gašić U, Stanojević SP, Tešić ŽL, Pešić MB. Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants. 2022;11(11):2164.
doi:10.3390/antiox11112164 .

Milinčić, Danijel D, Stanisavljević, Nemanja S, Kostić, Aleksandar Ž, Gašić, Uroš, Stanojević, Slađana P, Tešić, Živoslav Lj, Pešić, Mirjana B, "Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion" in Antioxidants, 11, no. 11 (2022):2164,
https://doi.org/10.3390/antiox11112164 . .

TY  - JOUR
AU  - Horvacki, Nikola
AU  - Andrić, Filip
AU  - Gašić, Uroš
AU  - Đurović, Dejan
AU  - Tešić, Živoslav
AU  - Fotirić Akšić, Milica
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9653822
UR  - https://www.mdpi.com/1420-3049/27/21/7651
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5239
AB  - Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.
PB  - Basel: MDPI
T2  - Molecules
T1  - Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia
IS  - 21
VL  - 27
DO  - 10.3390/molecules27217651
SP  - 7651
ER  - 

@article{
author = "Horvacki, Nikola and Andrić, Filip and Gašić, Uroš and Đurović, Dejan and Tešić, Živoslav and Fotirić Akšić, Milica and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia",
number = "21",
volume = "27",
doi = "10.3390/molecules27217651",
pages = "7651"
}

Horvacki, N., Andrić, F., Gašić, U., Đurović, D., Tešić, Ž., Fotirić Akšić, M.,& Milojković-Opsenica, D.. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules
Basel: MDPI., 27(21), 7651.
https://doi.org/10.3390/molecules27217651

Horvacki N, Andrić F, Gašić U, Đurović D, Tešić Ž, Fotirić Akšić M, Milojković-Opsenica D. Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules. 2022;27(21):7651.
doi:10.3390/molecules27217651 .

Horvacki, Nikola, Andrić, Filip, Gašić, Uroš, Đurović, Dejan, Tešić, Živoslav, Fotirić Akšić, Milica, Milojković-Opsenica, Dušanka, "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia" in Molecules, 27, no. 21 (2022):7651,
https://doi.org/10.3390/molecules27217651 . .

TY  - JOUR
AU  - Katanić Stanković, Jelena S.
AU  - Mićanović, Nenad
AU  - Grozdanić, Nađa
AU  - Kostić, Aleksandar Ž.
AU  - Gašić, Uroš
AU  - Stanojković, Tatjana
AU  - Popović-Đorđević, Jelena B.
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/11/1053
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5241
AB  - Plant-based food represents an excellent source of different nutrients and bioactive compounds, such as phenolics, carotenoids, vitamins, etc., with proven health benefits for humans. The content of selected phytochemicals, polyphenolic profile, and biological activity (antioxidant potential and α-glucosidase inhibitory activity) of fruit extracts of medlar (Mespilus germanica L.), blackthorn (Prunus spinosa L.), and common hawthorn (Crataegus monogyna Jacq.), the neglected Rosaceae species originated from Serbia were studied. Targeted UHPLC/(−)HESI–MS/MS quantitative analysis of phenolic compounds revealed pinocembrin only in medlar fruit extract, and it is the first report of this flavanone in medlar fruits. Total phenolic content did not differ between extracts, whereas significant differences were observed for the contents of total flavonoids, total phenolic acids, and total gallotannins. Monomeric anthocyanins and total anthocyanins were significantly higher in blackthorn compared to medlar and hawthorn fruit extracts (p < 0.05). DPPH· and ABTS·+ scavenging activities for examined fruits were modest compared to other natural antioxidants and BHT. The most potent inhibitory activity toward α-glucosidase expressed medlar and blackthorn extracts with IC50 values of 129.46 and 199.84 μg/mL, respectively, which was higher compared to the standard drug acarbose.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia
IS  - 11
VL  - 8
DO  - 10.3390/horticulturae8111053
SP  - 1053
ER  - 

@article{
author = "Katanić Stanković, Jelena S. and Mićanović, Nenad and Grozdanić, Nađa and Kostić, Aleksandar Ž. and Gašić, Uroš and Stanojković, Tatjana and Popović-Đorđević, Jelena B.",
year = "2022",
abstract = "Plant-based food represents an excellent source of different nutrients and bioactive compounds, such as phenolics, carotenoids, vitamins, etc., with proven health benefits for humans. The content of selected phytochemicals, polyphenolic profile, and biological activity (antioxidant potential and α-glucosidase inhibitory activity) of fruit extracts of medlar (Mespilus germanica L.), blackthorn (Prunus spinosa L.), and common hawthorn (Crataegus monogyna Jacq.), the neglected Rosaceae species originated from Serbia were studied. Targeted UHPLC/(−)HESI–MS/MS quantitative analysis of phenolic compounds revealed pinocembrin only in medlar fruit extract, and it is the first report of this flavanone in medlar fruits. Total phenolic content did not differ between extracts, whereas significant differences were observed for the contents of total flavonoids, total phenolic acids, and total gallotannins. Monomeric anthocyanins and total anthocyanins were significantly higher in blackthorn compared to medlar and hawthorn fruit extracts (p < 0.05). DPPH· and ABTS·+ scavenging activities for examined fruits were modest compared to other natural antioxidants and BHT. The most potent inhibitory activity toward α-glucosidase expressed medlar and blackthorn extracts with IC50 values of 129.46 and 199.84 μg/mL, respectively, which was higher compared to the standard drug acarbose.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia",
number = "11",
volume = "8",
doi = "10.3390/horticulturae8111053",
pages = "1053"
}

Katanić Stanković, J. S., Mićanović, N., Grozdanić, N., Kostić, A. Ž., Gašić, U., Stanojković, T.,& Popović-Đorđević, J. B.. (2022). Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia. in Horticulturae
Basel: MDPI., 8(11), 1053.
https://doi.org/10.3390/horticulturae8111053

Katanić Stanković JS, Mićanović N, Grozdanić N, Kostić AŽ, Gašić U, Stanojković T, Popović-Đorđević JB. Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia. in Horticulturae. 2022;8(11):1053.
doi:10.3390/horticulturae8111053 .

Katanić Stanković, Jelena S., Mićanović, Nenad, Grozdanić, Nađa, Kostić, Aleksandar Ž., Gašić, Uroš, Stanojković, Tatjana, Popović-Đorđević, Jelena B., "Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia" in Horticulturae, 8, no. 11 (2022):1053,
https://doi.org/10.3390/horticulturae8111053 . .

TY  - JOUR
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Momčilović, Ivana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5122
AB  - Aeroponics is a modern and soilless technology that is used for the efficient production of pre-basic seed potatoes, namely minitubers. This study aimed to evaluate the effects of the cultivar and type of planting material on the production of minitubers in the aeroponic facility in Guča, Serbia, at short, 7-day harvest intervals. Although aeroponic cultivation prolonged the vegetative cycle in all five investigated cultivars, the dynamics of minituber formation varied between genotypes. Two early maturing cultivars, Cleopatra and Sinora, quickly completed the vegetative cycle and formed a small number of minitubers, while the medium-late to late cultivars, Kennebec and Agria, steadily tuberized during the entire cultivation period in the aeroponic facility. The type of planting material affected the dynamics of minituber formation in three investigated cultivars. Sinora, Cleopatra, and Désirée’s plants of in vitro origin reached the final number of minitubers and the vines started senescing much earlier than plants of minituber origin. Kennebec and Agria plants of in vitro origin produced the largest number of minitubers (53.8–54.5) and showed the highest yield (9.8–10.5 kg m−2) during the cultivation period, while the heaviest minitubers were formed by Sinora plants of minituber origin (15.48 g). In addition, the temperature during pre-harvest periods significantly affected the number of tubers at harvests in Kennebec and Agria, and minituber mass in Désirée.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Potato aeroponics: effects of cultivar and plant origin on minituber production
IS  - 10
VL  - 8
DO  - 10.3390/horticulturae8100915
SP  - 915
ER  - 

@article{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Momčilović, Ivana",
year = "2022",
abstract = "Aeroponics is a modern and soilless technology that is used for the efficient production of pre-basic seed potatoes, namely minitubers. This study aimed to evaluate the effects of the cultivar and type of planting material on the production of minitubers in the aeroponic facility in Guča, Serbia, at short, 7-day harvest intervals. Although aeroponic cultivation prolonged the vegetative cycle in all five investigated cultivars, the dynamics of minituber formation varied between genotypes. Two early maturing cultivars, Cleopatra and Sinora, quickly completed the vegetative cycle and formed a small number of minitubers, while the medium-late to late cultivars, Kennebec and Agria, steadily tuberized during the entire cultivation period in the aeroponic facility. The type of planting material affected the dynamics of minituber formation in three investigated cultivars. Sinora, Cleopatra, and Désirée’s plants of in vitro origin reached the final number of minitubers and the vines started senescing much earlier than plants of minituber origin. Kennebec and Agria plants of in vitro origin produced the largest number of minitubers (53.8–54.5) and showed the highest yield (9.8–10.5 kg m−2) during the cultivation period, while the heaviest minitubers were formed by Sinora plants of minituber origin (15.48 g). In addition, the temperature during pre-harvest periods significantly affected the number of tubers at harvests in Kennebec and Agria, and minituber mass in Désirée.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Potato aeroponics: effects of cultivar and plant origin on minituber production",
number = "10",
volume = "8",
doi = "10.3390/horticulturae8100915",
pages = "915"
}

Broćić, Z., Oljača, J., Pantelić, D., Rudić, J.,& Momčilović, I.. (2022). Potato aeroponics: effects of cultivar and plant origin on minituber production. in Horticulturae
Basel: MDPI., 8(10), 915.
https://doi.org/10.3390/horticulturae8100915

Broćić Z, Oljača J, Pantelić D, Rudić J, Momčilović I. Potato aeroponics: effects of cultivar and plant origin on minituber production. in Horticulturae. 2022;8(10):915.
doi:10.3390/horticulturae8100915 .

Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Momčilović, Ivana, "Potato aeroponics: effects of cultivar and plant origin on minituber production" in Horticulturae, 8, no. 10 (2022):915,
https://doi.org/10.3390/horticulturae8100915 . .

TY  - JOUR
AU  - Krajnović, Tamara
AU  - Pantelić, Nebojša Đ.
AU  - Wolf, Katharina
AU  - Eichhorn, Thomas
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Wessjohann, Ludger A.
AU  - Kaluđerović, Goran N.
PY  - 2022
UR  - https://www.mdpi.com/1996-1944/15/14/5028
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9320346
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5089
AB  - Xanthohumol (XN) and isoxanthohumol (IXN), prenylated flavonoids from Humulus lupulus, have been shown to possess antitumor/cancerprotective, antioxidant, antiinflammatory, and antiangiogenic properties. In this study, mesoporous silica (SBA-15) was loaded with different amounts of xanthohumol and isoxanthohumol and characterized by standard analytical methods. The anticancer potential of XN and IXN loaded into SBA-15 has been evaluated against malignant mouse melanoma B16F10 cells. When these cells were treated with SBA-15 containing xanthohumol, an increase of the activity correlated with a higher immobilization rate of XN was observed. Considering the amount of XN loaded into SBA-15 (calculated from TGA), an improved antitumor potential of XN was observed (IC50 = 10.8 ± 0.4 and 11.8 ± 0.5 µM for SBA-15|XN2 and SBA-15|XN3, respectively; vs. IC50 = 18.5 ± 1.5 µM for free XN). The main mechanism against tumor cells of immobilized XN includes inhibition of proliferation and autophagic cell death. The MC50 values for SBA-15 loaded with isoxanthohumol were over 300 µg/mL in all cases investigated.
PB  - Basel: MDPI
T2  - Materials (Basel, Switzerland)
T1  - Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells.
IS  - 14
VL  - 15
DO  - 10.3390/ma15145028
SP  - 5028
ER  - 

@article{
author = "Krajnović, Tamara and Pantelić, Nebojša Đ. and Wolf, Katharina and Eichhorn, Thomas and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Wessjohann, Ludger A. and Kaluđerović, Goran N.",
year = "2022",
abstract = "Xanthohumol (XN) and isoxanthohumol (IXN), prenylated flavonoids from Humulus lupulus, have been shown to possess antitumor/cancerprotective, antioxidant, antiinflammatory, and antiangiogenic properties. In this study, mesoporous silica (SBA-15) was loaded with different amounts of xanthohumol and isoxanthohumol and characterized by standard analytical methods. The anticancer potential of XN and IXN loaded into SBA-15 has been evaluated against malignant mouse melanoma B16F10 cells. When these cells were treated with SBA-15 containing xanthohumol, an increase of the activity correlated with a higher immobilization rate of XN was observed. Considering the amount of XN loaded into SBA-15 (calculated from TGA), an improved antitumor potential of XN was observed (IC50 = 10.8 ± 0.4 and 11.8 ± 0.5 µM for SBA-15|XN2 and SBA-15|XN3, respectively; vs. IC50 = 18.5 ± 1.5 µM for free XN). The main mechanism against tumor cells of immobilized XN includes inhibition of proliferation and autophagic cell death. The MC50 values for SBA-15 loaded with isoxanthohumol were over 300 µg/mL in all cases investigated.",
publisher = "Basel: MDPI",
journal = "Materials (Basel, Switzerland)",
title = "Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells.",
number = "14",
volume = "15",
doi = "10.3390/ma15145028",
pages = "5028"
}

Krajnović, T., Pantelić, N. Đ., Wolf, K., Eichhorn, T., Maksimović-Ivanić, D., Mijatović, S., Wessjohann, L. A.,& Kaluđerović, G. N.. (2022). Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells.. in Materials (Basel, Switzerland)
Basel: MDPI., 15(14), 5028.
https://doi.org/10.3390/ma15145028

Krajnović T, Pantelić NĐ, Wolf K, Eichhorn T, Maksimović-Ivanić D, Mijatović S, Wessjohann LA, Kaluđerović GN. Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells.. in Materials (Basel, Switzerland). 2022;15(14):5028.
doi:10.3390/ma15145028 .

Krajnović, Tamara, Pantelić, Nebojša Đ., Wolf, Katharina, Eichhorn, Thomas, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Wessjohann, Ludger A., Kaluđerović, Goran N., "Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells." in Materials (Basel, Switzerland), 15, no. 14 (2022):5028,
https://doi.org/10.3390/ma15145028 . .

TY  - JOUR
AU  - Vidović, Biljana
AU  - Anđelković, Nikola
AU  - Jojić, Vida
AU  - Cvrković, Tatjana
AU  - Petanović, Radmila
AU  - Marini, Francesca
AU  - Cristofaro, Massimo
AU  - Rector, Brian G.
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5068
AB  - Simple Summary: Natural enemies of cheatgrass, an invasive plant in the western USA that is
associated with recent increases in destructive wildfires, were sought as part of a classical biological
control program targeting that plant. A population of mites was discovered infesting cheatgrass
plants in central Bulgaria and determined to be a new species using morphological, morphometric
analysis (i.e., measurement of specific body parts) and DNA barcoding (i.e., comparison of DNA
sequences in genes shared by all mite species). Without these modern analytical tools, this mite
would probably have been considered as the same species as another mite that infests other plants.
This new mite species will now be tested to determine if it is suitable for importation and release in
the USA as a biological control agent of cheatgrass.
Abstract: A new species of eriophyoid mite, Aculodes marcelli sp. nov., was discovered on cheatgrass,
Anisantha tectorum (L.) Nevski (syn. Bromus tectorum L.), an annual grass that is native to Eurasia and
Northern Africa. This grass was introduced to North America near the end of the 19th century and
now is widespread and associated with the observed increases in the size, frequency, and intensity of
wildfires in western N. America. In this paper, A. marcelli sp. nov., is morphologically described and
illustrated. Compared with other Aculodes spp., it differs based on morphology and the sequence
of the mitochondrial cytochrome oxidase gene, subunit I (MT-CO1). Results of morphometric
analysis showed clear differentiation between A. marcelli sp. nov., and the most similar congener, A.
altamurgiensis from Taeniatherum caput-medusae. Analysis of MT-CO1 sequence divergence revealed
significant levels of genetic variation (17.7%) and supported the results from the morphometric
analysis; therefore, it is determined that they are two different species. Aculodes marcelli sp. nov., is a
new candidate agent for classical biological control of A. tectorum.
PB  - Basel: MDPI
T2  - Insects
T1  - A New Aculodes Species (Prostigmata: Eriophyidae) Described from an Invasive Weed by Morphological, Morphometric and DNA Barcode Analyses
IS  - 10
VL  - 13
DO  - 10.3390/insects13100877
SP  - 877
ER  - 

@article{
author = "Vidović, Biljana and Anđelković, Nikola and Jojić, Vida and Cvrković, Tatjana and Petanović, Radmila and Marini, Francesca and Cristofaro, Massimo and Rector, Brian G.",
year = "2022",
abstract = "Simple Summary: Natural enemies of cheatgrass, an invasive plant in the western USA that is
associated with recent increases in destructive wildfires, were sought as part of a classical biological
control program targeting that plant. A population of mites was discovered infesting cheatgrass
plants in central Bulgaria and determined to be a new species using morphological, morphometric
analysis (i.e., measurement of specific body parts) and DNA barcoding (i.e., comparison of DNA
sequences in genes shared by all mite species). Without these modern analytical tools, this mite
would probably have been considered as the same species as another mite that infests other plants.
This new mite species will now be tested to determine if it is suitable for importation and release in
the USA as a biological control agent of cheatgrass.
Abstract: A new species of eriophyoid mite, Aculodes marcelli sp. nov., was discovered on cheatgrass,
Anisantha tectorum (L.) Nevski (syn. Bromus tectorum L.), an annual grass that is native to Eurasia and
Northern Africa. This grass was introduced to North America near the end of the 19th century and
now is widespread and associated with the observed increases in the size, frequency, and intensity of
wildfires in western N. America. In this paper, A. marcelli sp. nov., is morphologically described and
illustrated. Compared with other Aculodes spp., it differs based on morphology and the sequence
of the mitochondrial cytochrome oxidase gene, subunit I (MT-CO1). Results of morphometric
analysis showed clear differentiation between A. marcelli sp. nov., and the most similar congener, A.
altamurgiensis from Taeniatherum caput-medusae. Analysis of MT-CO1 sequence divergence revealed
significant levels of genetic variation (17.7%) and supported the results from the morphometric
analysis; therefore, it is determined that they are two different species. Aculodes marcelli sp. nov., is a
new candidate agent for classical biological control of A. tectorum.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "A New Aculodes Species (Prostigmata: Eriophyidae) Described from an Invasive Weed by Morphological, Morphometric and DNA Barcode Analyses",
number = "10",
volume = "13",
doi = "10.3390/insects13100877",
pages = "877"
}

Vidović, B., Anđelković, N., Jojić, V., Cvrković, T., Petanović, R., Marini, F., Cristofaro, M.,& Rector, B. G.. (2022). A New Aculodes Species (Prostigmata: Eriophyidae) Described from an Invasive Weed by Morphological, Morphometric and DNA Barcode Analyses. in Insects
Basel: MDPI., 13(10), 877.
https://doi.org/10.3390/insects13100877

Vidović B, Anđelković N, Jojić V, Cvrković T, Petanović R, Marini F, Cristofaro M, Rector BG. A New Aculodes Species (Prostigmata: Eriophyidae) Described from an Invasive Weed by Morphological, Morphometric and DNA Barcode Analyses. in Insects. 2022;13(10):877.
doi:10.3390/insects13100877 .

Vidović, Biljana, Anđelković, Nikola, Jojić, Vida, Cvrković, Tatjana, Petanović, Radmila, Marini, Francesca, Cristofaro, Massimo, Rector, Brian G., "A New Aculodes Species (Prostigmata: Eriophyidae) Described from an Invasive Weed by Morphological, Morphometric and DNA Barcode Analyses" in Insects, 13, no. 10 (2022):877,
https://doi.org/10.3390/insects13100877 . .

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2210803322000495
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5012
AB  - Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
T2  - Journal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
VL  - 34
DO  - 10.1016/j.hermed.2022.100580
SP  - 100580
ER  - 

@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
journal = "Journal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
volume = "34",
doi = "10.1016/j.hermed.2022.100580",
pages = "100580"
}

Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž.,& Gašić, U.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine, 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580

Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić Ž, Gašić U. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .

Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav, Gašić, Uroš, "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Mačukanović-Jocić, Marina P
AU  - Milinčić, Danijel D.
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Gligorijević, Nevenka N.
AU  - Jarić, Snežana
AU  - Soković, Marina
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202200069
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35213785
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4941
AB  - The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin-3-O-rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.
T2  - Chemistry & Biodiversity
T1  - Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.
IS  - 4
VL  - 19
DO  - 10.1002/cbdv.202200069
SP  - e202200069
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Mačukanović-Jocić, Marina P and Milinčić, Danijel D. and Petrović, Jovana and Gašić, Uroš and Gligorijević, Nevenka N. and Jarić, Snežana and Soković, Marina and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2022",
abstract = "The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin-3-O-rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.",
journal = "Chemistry & Biodiversity",
title = "Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.",
number = "4",
volume = "19",
doi = "10.1002/cbdv.202200069",
pages = "e202200069"
}

Kostić, A. Ž., Mačukanović-Jocić, M. P., Milinčić, D. D., Petrović, J., Gašić, U., Gligorijević, N. N., Jarić, S., Soković, M., Tešić, Ž. Lj.,& Pešić, M. B.. (2022). Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.. in Chemistry & Biodiversity, 19(4), e202200069.
https://doi.org/10.1002/cbdv.202200069

Kostić AŽ, Mačukanović-Jocić MP, Milinčić DD, Petrović J, Gašić U, Gligorijević NN, Jarić S, Soković M, Tešić ŽL, Pešić MB. Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.. in Chemistry & Biodiversity. 2022;19(4):e202200069.
doi:10.1002/cbdv.202200069 .

Kostić, Aleksandar Ž., Mačukanović-Jocić, Marina P, Milinčić, Danijel D., Petrović, Jovana, Gašić, Uroš, Gligorijević, Nevenka N., Jarić, Snežana, Soković, Marina, Tešić, Živoslav Lj., Pešić, Mirjana B., "Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents." in Chemistry & Biodiversity, 19, no. 4 (2022):e202200069,
https://doi.org/10.1002/cbdv.202200069 . .

TY  - CONF
AU  - Subotić, Angelina
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Jerinić-Prodanović, Dušanka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5396
AB  - Биотички стрес, резултат интеракције између биљке и другог живог организма, 
укључујући и инсекте, има различите негативне ефекте на растење и развиће 
биљака. Циљ овог истраживања је био да се испитају физиолошки и биохемијски аспекти одговора биљака шаргарепе (Daucus carota) на стрес изаван исхраном 
мрквине буве (Bactericera trigonica). Поред директне исхране на биљкама, B.
trigonica је и вектор фастидиозне бактерије Candidatus Liberibacter solanacearum
која проузрокује различите болести код биљака из фамилије Apiaceae. Од 
одгајених имага B. trigonica изоловани су парови од по пет мужјака и женки, а 
потом су наношени на биљке шаргарепе које су претходно гајене у контролисаним условима. Делови листова биљака у експерименту узорковани су након једне и четири недеље, од инокулације инсеката, у циљу одређивања физиолошких и биохемијских одговора на биотички стрес. Код биљака шаргарепе, услед стреса насталог исхраном лисне буве, уочен је измењен садржај фотосинтетичких пигмената. Хистохемијском локализацијом праћена је продукција реактивних форми кисеоника у листовима инокулисаних биљака шаргарепе. Разлике у одговору појединих компоненти антиоксидативног метаболизма изаваних исхраном лисне буве могу бити адаптивна стратегија шаргарепе у одговору на примењени биотички стрес.
AB  - Biotički stres, rezultat interakcije između biljke i drugog živog organizma, uključujući i insekte, ima različite negativne efekte na rastenje i razviće biljaka. Cilj ovog istraživanja je bio da se ispitaju fiziološki i biohemijski aspekti odgovora biljaka šargarepe (Daucus carota) na stres izavan ishranom mrkvine buve (Bactericera trigonica). Pored direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Candidatus Liberibacter solanacearum koja prouzrokuje različite bolesti kod biljaka iz familije Apiaceae. Od odgajenih imaga B. trigonica izolovani su parovi od po pet mužjaka i ženki, a potom su nanošeni na biljke šargarepe koje su prethodno gajene u kontrolisanim uslovima. Delovi listova biljaka u eksperimentu uzorkovani su nakon jedne i četiri nedelje, od inokulacije insekata, u cilju određivanja fizioloških i biohemijskih odgovora na biotički stres. Kod biljaka šargarepe, usled stresa nastalog ishranom lisne buve, uočen je izmenjen sadržaj fotosintetičkih pigmenata. Histohemijskom lokalizacijom praćena je produkcija reaktivnih formi kiseonika u listovima inokulisanih biljaka šargarepe. Razlike u odgovoru pojedinih komponenti antioksidativnog metabolizma izavanih ishranom lisne buve mogu biti adaptivna strategija šargarepe u odgovoru na primenjeni biotički stres.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica
T1  - Fiziološki i biohemijski aspekti odgovora biljaka šargarepe na biotički stres izazvan ishranom mrkvine lisne buve Bactericera trigonica
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5396
ER  - 

@conference{
author = "Subotić, Angelina and Trifunović-Momčilov, Milana and Milošević, Snežana and Đurić, Marija and Jevremović, Slađana and Jerinić-Prodanović, Dušanka",
year = "2022",
abstract = "Биотички стрес, резултат интеракције између биљке и другог живог организма, 
укључујући и инсекте, има различите негативне ефекте на растење и развиће 
биљака. Циљ овог истраживања је био да се испитају физиолошки и биохемијски аспекти одговора биљака шаргарепе (Daucus carota) на стрес изаван исхраном 
мрквине буве (Bactericera trigonica). Поред директне исхране на биљкама, B.
trigonica је и вектор фастидиозне бактерије Candidatus Liberibacter solanacearum
која проузрокује различите болести код биљака из фамилије Apiaceae. Од 
одгајених имага B. trigonica изоловани су парови од по пет мужјака и женки, а 
потом су наношени на биљке шаргарепе које су претходно гајене у контролисаним условима. Делови листова биљака у експерименту узорковани су након једне и четири недеље, од инокулације инсеката, у циљу одређивања физиолошких и биохемијских одговора на биотички стрес. Код биљака шаргарепе, услед стреса насталог исхраном лисне буве, уочен је измењен садржај фотосинтетичких пигмената. Хистохемијском локализацијом праћена је продукција реактивних форми кисеоника у листовима инокулисаних биљака шаргарепе. Разлике у одговору појединих компоненти антиоксидативног метаболизма изаваних исхраном лисне буве могу бити адаптивна стратегија шаргарепе у одговору на примењени биотички стрес., Biotički stres, rezultat interakcije između biljke i drugog živog organizma, uključujući i insekte, ima različite negativne efekte na rastenje i razviće biljaka. Cilj ovog istraživanja je bio da se ispitaju fiziološki i biohemijski aspekti odgovora biljaka šargarepe (Daucus carota) na stres izavan ishranom mrkvine buve (Bactericera trigonica). Pored direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Candidatus Liberibacter solanacearum koja prouzrokuje različite bolesti kod biljaka iz familije Apiaceae. Od odgajenih imaga B. trigonica izolovani su parovi od po pet mužjaka i ženki, a potom su nanošeni na biljke šargarepe koje su prethodno gajene u kontrolisanim uslovima. Delovi listova biljaka u eksperimentu uzorkovani su nakon jedne i četiri nedelje, od inokulacije insekata, u cilju određivanja fizioloških i biohemijskih odgovora na biotički stres. Kod biljaka šargarepe, usled stresa nastalog ishranom lisne buve, uočen je izmenjen sadržaj fotosintetičkih pigmenata. Histohemijskom lokalizacijom praćena je produkcija reaktivnih formi kiseonika u listovima inokulisanih biljaka šargarepe. Razlike u odgovoru pojedinih komponenti antioksidativnog metabolizma izavanih ishranom lisne buve mogu biti adaptivna strategija šargarepe u odgovoru na primenjeni biotički stres.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica, Fiziološki i biohemijski aspekti odgovora biljaka šargarepe na biotički stres izazvan ishranom mrkvine lisne buve Bactericera trigonica",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5396"
}

Subotić, A., Trifunović-Momčilov, M., Milošević, S., Đurić, M., Jevremović, S.,& Jerinić-Prodanović, D.. (2022). Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 48.
https://hdl.handle.net/21.15107/rcub_ibiss_5396

Subotić A, Trifunović-Momčilov M, Milošević S, Đurić M, Jevremović S, Jerinić-Prodanović D. Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:48.
https://hdl.handle.net/21.15107/rcub_ibiss_5396 .

Subotić, Angelina, Trifunović-Momčilov, Milana, Milošević, Snežana, Đurić, Marija, Jevremović, Slađana, Jerinić-Prodanović, Dušanka, "Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):48,
https://hdl.handle.net/21.15107/rcub_ibiss_5396 .

TY  - CONF
AU  - Krajnović, Tamara
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Wessjohann, Ludger A.
AU  - Kaluđerović, Goran N.
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5289
AB  - Prenylflavonoid from hops - xanthohumol (XN) has been shown to possess diverse biological properties, including strong anticancer activity. One of the possibilities for improving delivery and effectiveness of drugs is the use of mesoporous silica nanoparticles such as nontoxic SBA-15. The aim of this study was the evaluation of the in vitro anticancer potential of XN loaded with different amounts into SBA-15 particles against malignant mouse melanoma B16-F10 cells. Our data indicate that SBA-15 containing XN showed a loading rate–activity dependence. Importantly, immobilization of XN into SBA-15 preserved and even potentiated its antitumor potential, in comparison to its free form. Also, by loading into SBA-15 carrier, XNs’ anticancer mode of action converted from predominantly cytotoxic to cytostatic, resulting in a reduction of dividing potential. In addition, contrasting the previously observed apoptotic-inducing property of free XN, immobilized XN induced autophagic cell death that might be important for disabling tumor repopulation in response to apoptotic-induced cell proliferation, a mechanism often asscociated with therapy failure of advanced forms of cancer1.
PB  - Belgrade: Faculty of Chemistry
C3  - Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia
T1  - Anticancer potential of xanthohumol loaded into SBA-15 mesoporous silica particles against B16-F10 cells
SP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5289
ER  - 

@conference{
author = "Krajnović, Tamara and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Wessjohann, Ludger A. and Kaluđerović, Goran N.",
year = "2022",
abstract = "Prenylflavonoid from hops - xanthohumol (XN) has been shown to possess diverse biological properties, including strong anticancer activity. One of the possibilities for improving delivery and effectiveness of drugs is the use of mesoporous silica nanoparticles such as nontoxic SBA-15. The aim of this study was the evaluation of the in vitro anticancer potential of XN loaded with different amounts into SBA-15 particles against malignant mouse melanoma B16-F10 cells. Our data indicate that SBA-15 containing XN showed a loading rate–activity dependence. Importantly, immobilization of XN into SBA-15 preserved and even potentiated its antitumor potential, in comparison to its free form. Also, by loading into SBA-15 carrier, XNs’ anticancer mode of action converted from predominantly cytotoxic to cytostatic, resulting in a reduction of dividing potential. In addition, contrasting the previously observed apoptotic-inducing property of free XN, immobilized XN induced autophagic cell death that might be important for disabling tumor repopulation in response to apoptotic-induced cell proliferation, a mechanism often asscociated with therapy failure of advanced forms of cancer1.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia",
title = "Anticancer potential of xanthohumol loaded into SBA-15 mesoporous silica particles against B16-F10 cells",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5289"
}

Krajnović, T., Maksimović-Ivanić, D., Mijatović, S., Wessjohann, L. A.,& Kaluđerović, G. N.. (2022). Anticancer potential of xanthohumol loaded into SBA-15 mesoporous silica particles against B16-F10 cells. in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia
Belgrade: Faculty of Chemistry., 87.
https://hdl.handle.net/21.15107/rcub_ibiss_5289

Krajnović T, Maksimović-Ivanić D, Mijatović S, Wessjohann LA, Kaluđerović GN. Anticancer potential of xanthohumol loaded into SBA-15 mesoporous silica particles against B16-F10 cells. in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia. 2022;:87.
https://hdl.handle.net/21.15107/rcub_ibiss_5289 .

Krajnović, Tamara, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Wessjohann, Ludger A., Kaluđerović, Goran N., "Anticancer potential of xanthohumol loaded into SBA-15 mesoporous silica particles against B16-F10 cells" in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia (2022):87,
https://hdl.handle.net/21.15107/rcub_ibiss_5289 .

TY  - CONF
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Rac, Vladislav
AU  - Vidović, Bojana
AU  - Soković, Marina
AU  - Petrović, Jovana
AU  - Pešić, Mirjana
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6573
AB  - In recent years, the manufacturing of functional food has gained a lot of attention. The most recent 
studies indicate that bovine milk proteins are suitable carriers for a whole range of bioactive 
substances, mainly because of their micellar structure.
The aim of our study was to examine the possibility of goat milk proteins (RM - raw skimmed milk 
and CN - casein fraction) to deliver A. blazei extracts (VA - hot water extract and GA - glucan 
extract) in order to use all the potential health benefits of this mushroom. Furthermore, the effect of 
thermal treatment (90⁰C, 10 min; TRM – thermally treated RM and TCN - thermally treated CN) on 
carrier properties was also evaluated. The mixture of different goat milk proteins and A. blazei
extracts were prepared and zeta-potential and particle size distribution were determined by a laser 
light-scattering particle size analyzer, using distilled water as a dispersant.
The best results were obtained with the RM and TRM mixtures. It can be concluded that changes in 
particle size and electric charge of the micellar surface occurring in goat milk after heating have a 
positive impact on carrier properties. The particle size of TRM mixtures increased by 13.3 to 15.7% 
compared to the size of TRM particles, whereas the zeta-potential of TRM/VA increased for 6.6% 
compared to that of TRM. TCNs did not follow this trend. The measurement of CNs particle size 
distribution showed the presence of significantly different sizes (coefficient of variation >30%), 
whereas the zeta-potential measurements were stable. These results imply that the measurements of 
zeta-potential cannot be interpreted without the particle size data.
PB  - Belgrade: University of Belgrad
C3  - Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia
T1  - Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6573
ER  - 

@conference{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Rac, Vladislav and Vidović, Bojana and Soković, Marina and Petrović, Jovana and Pešić, Mirjana",
year = "2021",
abstract = "In recent years, the manufacturing of functional food has gained a lot of attention. The most recent 
studies indicate that bovine milk proteins are suitable carriers for a whole range of bioactive 
substances, mainly because of their micellar structure.
The aim of our study was to examine the possibility of goat milk proteins (RM - raw skimmed milk 
and CN - casein fraction) to deliver A. blazei extracts (VA - hot water extract and GA - glucan 
extract) in order to use all the potential health benefits of this mushroom. Furthermore, the effect of 
thermal treatment (90⁰C, 10 min; TRM – thermally treated RM and TCN - thermally treated CN) on 
carrier properties was also evaluated. The mixture of different goat milk proteins and A. blazei
extracts were prepared and zeta-potential and particle size distribution were determined by a laser 
light-scattering particle size analyzer, using distilled water as a dispersant.
The best results were obtained with the RM and TRM mixtures. It can be concluded that changes in 
particle size and electric charge of the micellar surface occurring in goat milk after heating have a 
positive impact on carrier properties. The particle size of TRM mixtures increased by 13.3 to 15.7% 
compared to the size of TRM particles, whereas the zeta-potential of TRM/VA increased for 6.6% 
compared to that of TRM. TCNs did not follow this trend. The measurement of CNs particle size 
distribution showed the presence of significantly different sizes (coefficient of variation >30%), 
whereas the zeta-potential measurements were stable. These results imply that the measurements of 
zeta-potential cannot be interpreted without the particle size data.",
publisher = "Belgrade: University of Belgrad",
journal = "Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia",
title = "Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.",
pages = "145",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6573"
}

Popović Minić, D., Milinčić, D., Rac, V., Vidović, B., Soković, M., Petrović, J.,& Pešić, M.. (2021). Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.. in Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia
Belgrade: University of Belgrad., 145.
https://hdl.handle.net/21.15107/rcub_ibiss_6573

Popović Minić D, Milinčić D, Rac V, Vidović B, Soković M, Petrović J, Pešić M. Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.. in Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia. 2021;:145.
https://hdl.handle.net/21.15107/rcub_ibiss_6573 .

Popović Minić, Dušanka, Milinčić, Danijel, Rac, Vladislav, Vidović, Bojana, Soković, Marina, Petrović, Jovana, Pešić, Mirjana, "Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts." in Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia (2021):145,
https://hdl.handle.net/21.15107/rcub_ibiss_6573 .

TY  - CHAP
AU  - Broćić, Zoran
AU  - Momčilović, Ivana
AU  - Poštić, Dobrivoj
AU  - Oljača, Jasmina
AU  - Veljković, Biljana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4471
AB  - Commercial production of potato, Solanum tuberosum L., is based on vegetative propagation by seed tubers. High-quality seed material is obtained from potato plants grown from minitubers (pre-basic seed potatoes). Likewise, minitubers are produced by acclimation and growth on soil or solid substrate of previously in vitro-propagated, virus-free microplants or microtubers. Aeroponics is a modern, soilless technique for minitubers’ production. In the aeroponic cultivation system, foliage is grown under conventional conditions, while the underground stems and roots of potato plants are located in a dark chamber, module, suspended in the air, and supplied with water and nutrients through a nutrient solution dispersed in the form of fine mist particles. Minitubers (tubers of 5-25 mm in size) are produced on underground stems, namely stolons. Potato minituber propagation in aeroponics has significant advantages over other used systems or techniques. This system enables the production of a high number of minitubers per plant during the production cycle that can usually be repeated during the year. Besides, successive harvesting allows minitubers to reach the desired, uniform size. Tubers grown in an aeroponic system are well-protected from pests and soil-borne diseases. Due to the recirculation of nutrient solution, efficient usage of space, and minimal environmental pollution, aeroponics enables the production of minitubers in an environmentally friendly manner. This chapter summarizes the current knowledge of the aeroponic production of potatoes. The advantages and deficiencies of this interesting production technique are also discussed.
PB  - New York : Nova Science Publishers Inc.
T2  - The Potato Crop: Management, Production, and Food Security
T1  - Production of High-Quality Seed Potato by Aeroponics
DO  - 10.52305/RHLO1469
SP  - 25
EP  - 59
ER  - 

@inbook{
editor = "Villa, Pedro",
author = "Broćić, Zoran and Momčilović, Ivana and Poštić, Dobrivoj and Oljača, Jasmina and Veljković, Biljana",
year = "2021",
abstract = "Commercial production of potato, Solanum tuberosum L., is based on vegetative propagation by seed tubers. High-quality seed material is obtained from potato plants grown from minitubers (pre-basic seed potatoes). Likewise, minitubers are produced by acclimation and growth on soil or solid substrate of previously in vitro-propagated, virus-free microplants or microtubers. Aeroponics is a modern, soilless technique for minitubers’ production. In the aeroponic cultivation system, foliage is grown under conventional conditions, while the underground stems and roots of potato plants are located in a dark chamber, module, suspended in the air, and supplied with water and nutrients through a nutrient solution dispersed in the form of fine mist particles. Minitubers (tubers of 5-25 mm in size) are produced on underground stems, namely stolons. Potato minituber propagation in aeroponics has significant advantages over other used systems or techniques. This system enables the production of a high number of minitubers per plant during the production cycle that can usually be repeated during the year. Besides, successive harvesting allows minitubers to reach the desired, uniform size. Tubers grown in an aeroponic system are well-protected from pests and soil-borne diseases. Due to the recirculation of nutrient solution, efficient usage of space, and minimal environmental pollution, aeroponics enables the production of minitubers in an environmentally friendly manner. This chapter summarizes the current knowledge of the aeroponic production of potatoes. The advantages and deficiencies of this interesting production technique are also discussed.",
publisher = "New York : Nova Science Publishers Inc.",
journal = "The Potato Crop: Management, Production, and Food Security",
booktitle = "Production of High-Quality Seed Potato by Aeroponics",
doi = "10.52305/RHLO1469",
pages = "25-59"
}

Villa, P., Broćić, Z., Momčilović, I., Poštić, D., Oljača, J.,& Veljković, B.. (2021). Production of High-Quality Seed Potato by Aeroponics. in The Potato Crop: Management, Production, and Food Security
New York : Nova Science Publishers Inc.., 25-59.
https://doi.org/10.52305/RHLO1469

Villa P, Broćić Z, Momčilović I, Poštić D, Oljača J, Veljković B. Production of High-Quality Seed Potato by Aeroponics. in The Potato Crop: Management, Production, and Food Security. 2021;:25-59.
doi:10.52305/RHLO1469 .

Villa, Pedro, Broćić, Zoran, Momčilović, Ivana, Poštić, Dobrivoj, Oljača, Jasmina, Veljković, Biljana, "Production of High-Quality Seed Potato by Aeroponics" in The Potato Crop: Management, Production, and Food Security (2021):25-59,
https://doi.org/10.52305/RHLO1469 . .