TY  - JOUR
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Slavkovska, Violeta
AU  - Mihailov-Krstev, Tatjana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - http://link.springer.com/10.1007/s00425-018-03071-5
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3256
AB  - MAIN CONCLUSION In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.
PB  - Springer Berlin Heidelberg
T2  - Planta
T1  - Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).
DO  - 10.1007/s00425-018-03071-5
ER  - 

@article{
author = "Tošić, Svetlana and Stojičić, Dragana and Slavkovska, Violeta and Mihailov-Krstev, Tatjana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
abstract = "MAIN CONCLUSION In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.",
publisher = "Springer Berlin Heidelberg",
journal = "Planta",
title = "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).",
doi = "10.1007/s00425-018-03071-5"
}

Tošić, S., Stojičić, D., Slavkovska, V., Mihailov-Krstev, T., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).. in Planta
Springer Berlin Heidelberg..
https://doi.org/10.1007/s00425-018-03071-5

Tošić S, Stojičić D, Slavkovska V, Mihailov-Krstev T, Zlatković B, Budimir S, Uzelac B. Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).. in Planta. 2019;.
doi:10.1007/s00425-018-03071-5 .

Tošić, Svetlana, Stojičić, Dragana, Slavkovska, Violeta, Mihailov-Krstev, Tatjana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)." in Planta (2019),
https://doi.org/10.1007/s00425-018-03071-5 . .

TY  - CONF
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6735
AB  - Morphology and structure of Micromeria croatica glandular trichomes in relation to the secretory process were characterized using scanning electron microscopy (SEM) and light microscopy, and their secretion was histochemically analyzed. SEM and histochemical analysis revealed that leaf indumentum of in vitro grown M. croatica consisted of non-glandular and glandular trichomes (Figure 1a). The main types of glandular trichomes observed in M. croatica leaves were peltate and capitate (Figure 1b-d). Peltate trichomes consisted of a broad basal cell embedded in the epidermis, a single-celled stalk, and a large round multicellular head (Figure 1e, h). Two types of capitate trichomes, differing in size and structure, could be distinguished. Type I capitate trichomes were positioned at an angle to the leaf surface and composed of one basal epidermal cell, short cutinized stalk and unicellular ellipsoidal head (Figure 1f). Type II capitate trichomes comprised of one basal cell, unicellular stalk and one apical secretory cell (Figure 1g). Apical cell of mature type II capitate trichomes had well developed round subcuticular space (Figure 1d). Plant extracts secreted from glandular trichomes are a valuable source of biologically active compounds. Phytochemical analysis identified up to 37 compounds in the essential oils (EOs) of wild-growing and micropropagated plants, and revealed differences in both content and the composition of the EOs obtained from different samples. EOs from both native plant material and in vitro cultivated plants showed very high percentages of total monoterpenoids, dominated by oxygenated monoterpenes. 
Borneol was the main essential oil component detected in wild-growing plants (25.28%) and micropropagated plants grown on plant growth regulator-free medium (20.30%). Micropropagated plants cultivated in the presence of 0.3 µM kinetin had the highest yield of the EO, with geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%) being the main EO components. The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. Sesquiterpene hydrocarbons accounted for the majority of detected sesquiterpenoids and were dominated by alpha-cadinene and beta-vetivenene. Some of the volatiles were found only in plant material obtained by micropropagation but not in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties.
PB  - Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society
C3  - Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)
SP  - 302
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6735
ER  - 

@conference{
author = "Tošić, Svetlana and Stojičić, Dragana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2017",
abstract = "Morphology and structure of Micromeria croatica glandular trichomes in relation to the secretory process were characterized using scanning electron microscopy (SEM) and light microscopy, and their secretion was histochemically analyzed. SEM and histochemical analysis revealed that leaf indumentum of in vitro grown M. croatica consisted of non-glandular and glandular trichomes (Figure 1a). The main types of glandular trichomes observed in M. croatica leaves were peltate and capitate (Figure 1b-d). Peltate trichomes consisted of a broad basal cell embedded in the epidermis, a single-celled stalk, and a large round multicellular head (Figure 1e, h). Two types of capitate trichomes, differing in size and structure, could be distinguished. Type I capitate trichomes were positioned at an angle to the leaf surface and composed of one basal epidermal cell, short cutinized stalk and unicellular ellipsoidal head (Figure 1f). Type II capitate trichomes comprised of one basal cell, unicellular stalk and one apical secretory cell (Figure 1g). Apical cell of mature type II capitate trichomes had well developed round subcuticular space (Figure 1d). Plant extracts secreted from glandular trichomes are a valuable source of biologically active compounds. Phytochemical analysis identified up to 37 compounds in the essential oils (EOs) of wild-growing and micropropagated plants, and revealed differences in both content and the composition of the EOs obtained from different samples. EOs from both native plant material and in vitro cultivated plants showed very high percentages of total monoterpenoids, dominated by oxygenated monoterpenes. 
Borneol was the main essential oil component detected in wild-growing plants (25.28%) and micropropagated plants grown on plant growth regulator-free medium (20.30%). Micropropagated plants cultivated in the presence of 0.3 µM kinetin had the highest yield of the EO, with geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%) being the main EO components. The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. Sesquiterpene hydrocarbons accounted for the majority of detected sesquiterpenoids and were dominated by alpha-cadinene and beta-vetivenene. Some of the volatiles were found only in plant material obtained by micropropagation but not in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties.",
publisher = "Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society",
journal = "Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)",
pages = "302-303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6735"
}

Tošić, S., Stojičić, D., Slavkovska, V., Zlatković, B., Budimir, S.,& Uzelac, B.. (2017). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia
Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society., 302-303.
https://hdl.handle.net/21.15107/rcub_ibiss_6735

Tošić S, Stojičić D, Slavkovska V, Zlatković B, Budimir S, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia. 2017;:302-303.
https://hdl.handle.net/21.15107/rcub_ibiss_6735 .

Tošić, Svetlana, Stojičić, Dragana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)" in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia (2017):302-303,
https://hdl.handle.net/21.15107/rcub_ibiss_6735 .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana
AU  - Niketić, Marjan
PY  - 2017
UR  - http://link.springer.com/10.1007/s13197-017-2610-z
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2784
AB  - Many Heracleum L. taxa (Apiaceae) are used as food and spices, and in traditional medicine. In this work, the chemical composition of Heracleum pyrenaicum subsp. orsinii (Guss.) F. Pedrotti and Pignatti root, leaf and fruit essential oils, their antimicrobial activity and cytotoxic effect on malignant and normal cells were investigated for the first time. The composition of the oils was analyzed by GC and GC–MS. Monoterpenes prevailed in the root oil, with β-pinene (38.6%) being dominant, while in the leaf oil, sesquiterpenes, mostly (E)-nerolidol (20.5%) and (E)-caryophyllene (17.0%), were the most abundant constituents. The fruit oil contained the majority of aliphatic esters, mainly octyl acetate (36.8%) and octyl hexanoate (22.1%). The antimicrobial activity was determined by microdilution method against eight bacteria and eight fungi (standard strains, clinical or food isolates). The best antibacterial activity, better than the activity of ampicillin, was shown by the root oil against Salmonella typhimurium, Escherichia coli and Pseudomonas aeruginosa. The strongest antifungal activity, stronger than the activity of ketoconazole, was exhibited by the leaf and root oils against Trichoderma viride, and by the root oil against Aspergillus ochraceus. The cytotoxic effect of the oils, determined by MTT test, was prominent against malignant HeLa, LS174 and A549 cells (IC50 = 6.49–14.56 μg/mL). On the other hand, the oils did not show toxicity against normal MRC-5 cells at tested concentrations (IC50 > 200.00 μg/mL). It can be concluded that investigated H. pyrenaicum subsp. orsinii oils represent potential new raw materials for food and pharmaceutical industry.
T2  - Journal of Food Science and Technology
T1  - Edible wild plant Heracleum pyrenaicum subsp. orsinii as a potential new source of bioactive essential oils
IS  - 8
VL  - 54
DO  - 10.1007/s13197-017-2610-z
SP  - 2193
EP  - 2202
ER  - 

@article{
author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana and Niketić, Marjan",
year = "2017",
abstract = "Many Heracleum L. taxa (Apiaceae) are used as food and spices, and in traditional medicine. In this work, the chemical composition of Heracleum pyrenaicum subsp. orsinii (Guss.) F. Pedrotti and Pignatti root, leaf and fruit essential oils, their antimicrobial activity and cytotoxic effect on malignant and normal cells were investigated for the first time. The composition of the oils was analyzed by GC and GC–MS. Monoterpenes prevailed in the root oil, with β-pinene (38.6%) being dominant, while in the leaf oil, sesquiterpenes, mostly (E)-nerolidol (20.5%) and (E)-caryophyllene (17.0%), were the most abundant constituents. The fruit oil contained the majority of aliphatic esters, mainly octyl acetate (36.8%) and octyl hexanoate (22.1%). The antimicrobial activity was determined by microdilution method against eight bacteria and eight fungi (standard strains, clinical or food isolates). The best antibacterial activity, better than the activity of ampicillin, was shown by the root oil against Salmonella typhimurium, Escherichia coli and Pseudomonas aeruginosa. The strongest antifungal activity, stronger than the activity of ketoconazole, was exhibited by the leaf and root oils against Trichoderma viride, and by the root oil against Aspergillus ochraceus. The cytotoxic effect of the oils, determined by MTT test, was prominent against malignant HeLa, LS174 and A549 cells (IC50 = 6.49–14.56 μg/mL). On the other hand, the oils did not show toxicity against normal MRC-5 cells at tested concentrations (IC50 > 200.00 μg/mL). It can be concluded that investigated H. pyrenaicum subsp. orsinii oils represent potential new raw materials for food and pharmaceutical industry.",
journal = "Journal of Food Science and Technology",
title = "Edible wild plant Heracleum pyrenaicum subsp. orsinii as a potential new source of bioactive essential oils",
number = "8",
volume = "54",
doi = "10.1007/s13197-017-2610-z",
pages = "2193-2202"
}

Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A.,& Niketić, M.. (2017). Edible wild plant Heracleum pyrenaicum subsp. orsinii as a potential new source of bioactive essential oils. in Journal of Food Science and Technology, 54(8), 2193-2202.
https://doi.org/10.1007/s13197-017-2610-z

Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić A, Niketić M. Edible wild plant Heracleum pyrenaicum subsp. orsinii as a potential new source of bioactive essential oils. in Journal of Food Science and Technology. 2017;54(8):2193-2202.
doi:10.1007/s13197-017-2610-z .

Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana, Niketić, Marjan, "Edible wild plant Heracleum pyrenaicum subsp. orsinii as a potential new source of bioactive essential oils" in Journal of Food Science and Technology, 54, no. 8 (2017):2193-2202,
https://doi.org/10.1007/s13197-017-2610-z . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana
AU  - Niketić, Marjan
PY  - 2017
UR  - http://xlink.rsc.org/?DOI=C6FO01698G
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2557
AB  - Although some widespread, native cow parsnips (Heracleum L. spp., Apiaceae) had broad medicinal and culinary applications throughout history, the knowledge about their volatile constituents is insufficient. This work investigates the composition and bioactivities of H. sphondylium L. (HSPH), H. sibiricum L. (HSIB) and H. montanum Schleich. ex Gaudin (HMON) essential oils. The composition was tested by GC and GC-MS. (Z)-β-Ocimene was the most abundant in HSPH (28.9%) and HMON (20.4%) root oils, while in HSIB root oil, β-pinene (26.2%), methyl eugenol (22.3%) and elemicin (25.6%) prevailed. Leaf and flower oils were dominated by various sesquiterpenes (germacrene D, β-sesquiphellandrene, (E)-β-farnesene and/or (E)-caryophyllene) and/or phenylpropanoids (apiole, methyl eugenol, elemicin and/or (Z)-isoelemicin). Octyl acetate (57.5-67.1%) was the main constituent of all fruit oils. The antimicrobial activity was screened by a microdilution method against eight bacteria and eight fungi. The strongest antimicrobial effect, in several cases better than the activity of antibiotics, was shown by HSPH (MICs = 0.12-3.30 mg mL-1) and HMON (MICs = 0.10-1.30 mg mL-1) flower oils against bacteria, and HSIB fruit oil against fungi (MICs = 0.15-0.40 mg mL-1). The MTT test revealed that the oils were not or weakly cytotoxic against human malignant HeLa, LS174 and/or A549 cells (except HSPH root oil; IC50 = 5.72-24.31 μg mL-1) and that tested oils were not toxic against human normal MRC-5 cells (at 200.00 μg mL-1). Significant activity observed against microorganisms that are the common cause of foodborne diseases, food contamination and/or hospital-acquired infections justifies certain traditional uses of the investigated plants and represents a good basis for further research of these Heracleum oils.
T2  - Food and Function
T1  - Essential oils of three cow parsnips – composition and activity against nosocomial and foodborne pathogens and food contaminants
IS  - 1
VL  - 8
DO  - 10.1039/C6FO01698G
SP  - 278
EP  - 290
ER  - 

@article{
author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana and Niketić, Marjan",
year = "2017",
abstract = "Although some widespread, native cow parsnips (Heracleum L. spp., Apiaceae) had broad medicinal and culinary applications throughout history, the knowledge about their volatile constituents is insufficient. This work investigates the composition and bioactivities of H. sphondylium L. (HSPH), H. sibiricum L. (HSIB) and H. montanum Schleich. ex Gaudin (HMON) essential oils. The composition was tested by GC and GC-MS. (Z)-β-Ocimene was the most abundant in HSPH (28.9%) and HMON (20.4%) root oils, while in HSIB root oil, β-pinene (26.2%), methyl eugenol (22.3%) and elemicin (25.6%) prevailed. Leaf and flower oils were dominated by various sesquiterpenes (germacrene D, β-sesquiphellandrene, (E)-β-farnesene and/or (E)-caryophyllene) and/or phenylpropanoids (apiole, methyl eugenol, elemicin and/or (Z)-isoelemicin). Octyl acetate (57.5-67.1%) was the main constituent of all fruit oils. The antimicrobial activity was screened by a microdilution method against eight bacteria and eight fungi. The strongest antimicrobial effect, in several cases better than the activity of antibiotics, was shown by HSPH (MICs = 0.12-3.30 mg mL-1) and HMON (MICs = 0.10-1.30 mg mL-1) flower oils against bacteria, and HSIB fruit oil against fungi (MICs = 0.15-0.40 mg mL-1). The MTT test revealed that the oils were not or weakly cytotoxic against human malignant HeLa, LS174 and/or A549 cells (except HSPH root oil; IC50 = 5.72-24.31 μg mL-1) and that tested oils were not toxic against human normal MRC-5 cells (at 200.00 μg mL-1). Significant activity observed against microorganisms that are the common cause of foodborne diseases, food contamination and/or hospital-acquired infections justifies certain traditional uses of the investigated plants and represents a good basis for further research of these Heracleum oils.",
journal = "Food and Function",
title = "Essential oils of three cow parsnips – composition and activity against nosocomial and foodborne pathogens and food contaminants",
number = "1",
volume = "8",
doi = "10.1039/C6FO01698G",
pages = "278-290"
}

Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A.,& Niketić, M.. (2017). Essential oils of three cow parsnips – composition and activity against nosocomial and foodborne pathogens and food contaminants. in Food and Function, 8(1), 278-290.
https://doi.org/10.1039/C6FO01698G

Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić A, Niketić M. Essential oils of three cow parsnips – composition and activity against nosocomial and foodborne pathogens and food contaminants. in Food and Function. 2017;8(1):278-290.
doi:10.1039/C6FO01698G .

Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana, Niketić, Marjan, "Essential oils of three cow parsnips – composition and activity against nosocomial and foodborne pathogens and food contaminants" in Food and Function, 8, no. 1 (2017):278-290,
https://doi.org/10.1039/C6FO01698G . .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Gligorijević, Nevenka
AU  - Čavić, Milena
AU  - Todorović, Slađana
AU  - Janković, Radmila
AU  - Ristić, Mihailo
AU  - Mišić, Danijela
AU  - Radulović, Siniša
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0926669017301309
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2609
AB  - Nepeta rtanjensis Diklić & Milojević (fam. Lamiaceae) is an endemic, critically endangered plant, protected by the law in Serbia. Various biological activities have been ascribed to its major constituents, nepetalactones. In this study we describe for the first time cytotoxic activity of N. rtanjensis essential oil (EO), obtained from field cultivated plants, which was found to be especially rich in trans,cis-nepetalactone. MTT assays indicated that after 72 h of treatment the EO exhibited cytotoxic activity on investigated cancer cell lines: HeLa, K562, A549, LS-174 and MDA-MB-231. Normal cell line (MRC-5) was the least sensitive to the treatment and IC50 value for this cell line was not reached within the tested range of EO concentrations (up to 0.1 μL/mL). Analysis of morphological changes of treated cells confirmed the higher sensitivity of tumor cells than normal cells to the tested EO. Application of N. rtanjensis EO resulted in the appearance of morphological changes in tested cancer cell lines characteristic for apoptotic cell death, and induced perturbations of the cell cycle of HeLa cells. In addition, upregulation of Bax and p53, and downregulation of Bcl-2, and Skp2 genes, involved in apoptotic signalling cascades, confirmed an apoptosis-inducing effect of N. rtanjensis EO on HeLa cells. Presented results highlighted the potential of N. rtanjensis EO in anticancer therapy.
T2  - Industrial Crops and Products
T1  - Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action
VL  - 100
DO  - 10.1016/j.indcrop.2017.02.027
SP  - 163
EP  - 170
ER  - 

@article{
author = "Skorić, Marijana and Gligorijević, Nevenka and Čavić, Milena and Todorović, Slađana and Janković, Radmila and Ristić, Mihailo and Mišić, Danijela and Radulović, Siniša",
year = "2017",
abstract = "Nepeta rtanjensis Diklić & Milojević (fam. Lamiaceae) is an endemic, critically endangered plant, protected by the law in Serbia. Various biological activities have been ascribed to its major constituents, nepetalactones. In this study we describe for the first time cytotoxic activity of N. rtanjensis essential oil (EO), obtained from field cultivated plants, which was found to be especially rich in trans,cis-nepetalactone. MTT assays indicated that after 72 h of treatment the EO exhibited cytotoxic activity on investigated cancer cell lines: HeLa, K562, A549, LS-174 and MDA-MB-231. Normal cell line (MRC-5) was the least sensitive to the treatment and IC50 value for this cell line was not reached within the tested range of EO concentrations (up to 0.1 μL/mL). Analysis of morphological changes of treated cells confirmed the higher sensitivity of tumor cells than normal cells to the tested EO. Application of N. rtanjensis EO resulted in the appearance of morphological changes in tested cancer cell lines characteristic for apoptotic cell death, and induced perturbations of the cell cycle of HeLa cells. In addition, upregulation of Bax and p53, and downregulation of Bcl-2, and Skp2 genes, involved in apoptotic signalling cascades, confirmed an apoptosis-inducing effect of N. rtanjensis EO on HeLa cells. Presented results highlighted the potential of N. rtanjensis EO in anticancer therapy.",
journal = "Industrial Crops and Products",
title = "Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action",
volume = "100",
doi = "10.1016/j.indcrop.2017.02.027",
pages = "163-170"
}

Skorić, M., Gligorijević, N., Čavić, M., Todorović, S., Janković, R., Ristić, M., Mišić, D.,& Radulović, S.. (2017). Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action. in Industrial Crops and Products, 100, 163-170.
https://doi.org/10.1016/j.indcrop.2017.02.027

Skorić M, Gligorijević N, Čavić M, Todorović S, Janković R, Ristić M, Mišić D, Radulović S. Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action. in Industrial Crops and Products. 2017;100:163-170.
doi:10.1016/j.indcrop.2017.02.027 .

Skorić, Marijana, Gligorijević, Nevenka, Čavić, Milena, Todorović, Slađana, Janković, Radmila, Ristić, Mihailo, Mišić, Danijela, Radulović, Siniša, "Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action" in Industrial Crops and Products, 100 (2017):163-170,
https://doi.org/10.1016/j.indcrop.2017.02.027 . .

TY  - JOUR
AU  - Fotopoulou, Theano
AU  - Ćirić, Ana
AU  - Kritsi, Eftichia
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C.F.R.
AU  - Soković, Marina
AU  - Zoumpoulakis, Panagiotis
AU  - Koufaki, Maria
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6747
AB  - Natural b-thujaplicin displays a remarkable array of biological activities for the prevention or
treatment of various disorders while its tropolone scaffold inspired the synthesis of new analogs. The
main goal of the current study was to evaluate the influence of 4-substituted piperazine moieties at
position 7 of the b-thujaplicin scaffold, on the antimicrobial activity. In order to determine the
biological activity of the b-thujaplicin derivatives, a microdilution method was used against a wide
variety of bacteria and fungi. Pseudomonas aeruginosa PAO 1 was used for testing antiquorum and
antibiofilm effects. Four human tumor cell lines (MCF-7, NCI-H460, HeLa, and HepG2) and a porcine
liver derived cell line (PLP2) were used for testing antitumor and cytotoxic activity. The compounds
present better antibacterial and antifungal activity in comparison with approved antimicrobials used
as control agents. b-Thujaplicin showed strong antibacterial and antifungal activities against all
tested species. Further studies of their antibacterial activity revealed that all compounds presented
good antibiofilm and antiquorum effects. Fungi were more susceptible than bacteria to the tested
compounds, with the exception of MK150, which possessed the best antibacterial effect. None of the
tested compounds, at the GI50 values obtained for the tumor cell lines, have shown toxicity for non tumor liver cells (PLP2). The prediction of physicochemical properties of the compounds was
performed to further explain the structure–activity relationship. Finally, in order to explore a possible
mechanism of action of the synthesized compounds, molecular docking studies were performed on
CYP51 (14-a lanosterol demethylase), an important component of the fungal cell membrane.
PB  - Weinheim, Germany: WILEY-VCH Verlag GmbH & Co. KGaA,
T2  - Archiv der Pharmazie
T1  - Antimicrobial/Antibiofilm Activity and Cytotoxic Studies of b-Thujaplicin Derivatives
IS  - 9
VL  - 349
DO  - 10.1002/ardp.201600095
SP  - 698
EP  - 709
ER  - 

@article{
author = "Fotopoulou, Theano and Ćirić, Ana and Kritsi, Eftichia and Calhelha, Ricardo C. and Ferreira, Isabel C.F.R. and Soković, Marina and Zoumpoulakis, Panagiotis and Koufaki, Maria",
year = "2016",
abstract = "Natural b-thujaplicin displays a remarkable array of biological activities for the prevention or
treatment of various disorders while its tropolone scaffold inspired the synthesis of new analogs. The
main goal of the current study was to evaluate the influence of 4-substituted piperazine moieties at
position 7 of the b-thujaplicin scaffold, on the antimicrobial activity. In order to determine the
biological activity of the b-thujaplicin derivatives, a microdilution method was used against a wide
variety of bacteria and fungi. Pseudomonas aeruginosa PAO 1 was used for testing antiquorum and
antibiofilm effects. Four human tumor cell lines (MCF-7, NCI-H460, HeLa, and HepG2) and a porcine
liver derived cell line (PLP2) were used for testing antitumor and cytotoxic activity. The compounds
present better antibacterial and antifungal activity in comparison with approved antimicrobials used
as control agents. b-Thujaplicin showed strong antibacterial and antifungal activities against all
tested species. Further studies of their antibacterial activity revealed that all compounds presented
good antibiofilm and antiquorum effects. Fungi were more susceptible than bacteria to the tested
compounds, with the exception of MK150, which possessed the best antibacterial effect. None of the
tested compounds, at the GI50 values obtained for the tumor cell lines, have shown toxicity for non tumor liver cells (PLP2). The prediction of physicochemical properties of the compounds was
performed to further explain the structure–activity relationship. Finally, in order to explore a possible
mechanism of action of the synthesized compounds, molecular docking studies were performed on
CYP51 (14-a lanosterol demethylase), an important component of the fungal cell membrane.",
publisher = "Weinheim, Germany: WILEY-VCH Verlag GmbH & Co. KGaA,",
journal = "Archiv der Pharmazie",
title = "Antimicrobial/Antibiofilm Activity and Cytotoxic Studies of b-Thujaplicin Derivatives",
number = "9",
volume = "349",
doi = "10.1002/ardp.201600095",
pages = "698-709"
}

Fotopoulou, T., Ćirić, A., Kritsi, E., Calhelha, R. C., Ferreira, I. C.F.R., Soković, M., Zoumpoulakis, P.,& Koufaki, M.. (2016). Antimicrobial/Antibiofilm Activity and Cytotoxic Studies of b-Thujaplicin Derivatives. in Archiv der Pharmazie
Weinheim, Germany: WILEY-VCH Verlag GmbH & Co. KGaA,., 349(9), 698-709.
https://doi.org/10.1002/ardp.201600095

Fotopoulou T, Ćirić A, Kritsi E, Calhelha RC, Ferreira IC, Soković M, Zoumpoulakis P, Koufaki M. Antimicrobial/Antibiofilm Activity and Cytotoxic Studies of b-Thujaplicin Derivatives. in Archiv der Pharmazie. 2016;349(9):698-709.
doi:10.1002/ardp.201600095 .

Fotopoulou, Theano, Ćirić, Ana, Kritsi, Eftichia, Calhelha, Ricardo C., Ferreira, Isabel C.F.R., Soković, Marina, Zoumpoulakis, Panagiotis, Koufaki, Maria, "Antimicrobial/Antibiofilm Activity and Cytotoxic Studies of b-Thujaplicin Derivatives" in Archiv der Pharmazie, 349, no. 9 (2016):698-709,
https://doi.org/10.1002/ardp.201600095 . .

TY  - JOUR
AU  - Petrović, Nada V.
AU  - Petrović, Slobodan S.
AU  - Ristić, Mihailo S.
AU  - Džamić, Ana M.
AU  - Ćirić, Ana
PY  - 2016
UR  - http://www.acgpubs.org/article/records-of-natural-products/2016/5-september-october/chemical-composition-and-antimicrobial-activity-of-thymus-praecox-opiz-ssp-polytrichus-essential-oil-from-serbia
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6750
AB  - Chemical composition and antimicrobial activity of the essential oil of wild growing Thymus praecox Opiz ssp. polytrichus were studied. trans-Nerolidol (19.79%), germacrene D (18.48%) and thymol (9.62%) were the main components in essential oil. This study is the first report of the antimicrobial activity of essential oil obtained from the T. praecox Opiz ssp. polytrichus. Antimicrobial activity of essential oil was investigated on Bacillus cereus, Micrococcus flavus, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Salmonella typhimurium, Aspergillus fumigatus, A. versicolor, A. ochraceus, A. niger, Trichoderma viride, Penicillium funiculosum, P. ochrochloron, and P. verrucosum var. cyclopium strains. In the antimicrobial assays, essential oil showed high antimicrobial potential (MIC 19–150 m g/mL, MBC 39–300 m g/mL for bacteria; and MIC 19.5–39 m g/mL, MFC 39–78 m g/mL for fungi).
PB  - ACG Publications
T2  - Records of Natural Products
T1  - Chemical Composition and Antimicrobial Activity of Thymus  praecox Opiz ssp. polytrichus Essential Oil from Serbia
IS  - 5
VL  - 10
SP  - 633
EP  - 638
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6750
ER  - 

@article{
author = "Petrović, Nada V. and Petrović, Slobodan S. and Ristić, Mihailo S. and Džamić, Ana M. and Ćirić, Ana",
year = "2016",
abstract = "Chemical composition and antimicrobial activity of the essential oil of wild growing Thymus praecox Opiz ssp. polytrichus were studied. trans-Nerolidol (19.79%), germacrene D (18.48%) and thymol (9.62%) were the main components in essential oil. This study is the first report of the antimicrobial activity of essential oil obtained from the T. praecox Opiz ssp. polytrichus. Antimicrobial activity of essential oil was investigated on Bacillus cereus, Micrococcus flavus, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Salmonella typhimurium, Aspergillus fumigatus, A. versicolor, A. ochraceus, A. niger, Trichoderma viride, Penicillium funiculosum, P. ochrochloron, and P. verrucosum var. cyclopium strains. In the antimicrobial assays, essential oil showed high antimicrobial potential (MIC 19–150 m g/mL, MBC 39–300 m g/mL for bacteria; and MIC 19.5–39 m g/mL, MFC 39–78 m g/mL for fungi).",
publisher = "ACG Publications",
journal = "Records of Natural Products",
title = "Chemical Composition and Antimicrobial Activity of Thymus  praecox Opiz ssp. polytrichus Essential Oil from Serbia",
number = "5",
volume = "10",
pages = "633-638",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6750"
}

Petrović, N. V., Petrović, S. S., Ristić, M. S., Džamić, A. M.,& Ćirić, A.. (2016). Chemical Composition and Antimicrobial Activity of Thymus  praecox Opiz ssp. polytrichus Essential Oil from Serbia. in Records of Natural Products
ACG Publications., 10(5), 633-638.
https://hdl.handle.net/21.15107/rcub_ibiss_6750

Petrović NV, Petrović SS, Ristić MS, Džamić AM, Ćirić A. Chemical Composition and Antimicrobial Activity of Thymus  praecox Opiz ssp. polytrichus Essential Oil from Serbia. in Records of Natural Products. 2016;10(5):633-638.
https://hdl.handle.net/21.15107/rcub_ibiss_6750 .

Petrović, Nada V., Petrović, Slobodan S., Ristić, Mihailo S., Džamić, Ana M., Ćirić, Ana, "Chemical Composition and Antimicrobial Activity of Thymus  praecox Opiz ssp. polytrichus Essential Oil from Serbia" in Records of Natural Products, 10, no. 5 (2016):633-638,
https://hdl.handle.net/21.15107/rcub_ibiss_6750 .

TY  - JOUR
AU  - Savran, Ahmet
AU  - Zengin, Gokhan
AU  - Aktumsek, Abdurrahman
AU  - Mocan, Andrei
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Soković, Marina
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6746
AB  - The present study outlines a chemical characterization and further effects beneficial to health of edible
Rumex scutatus and Pseudosempervivum sempervivum, in addition to presenting the antioxidant,
enzyme inhibitory effects and antimicrobial properties of different extracts. The phenolic compounds
composition of the extracts was assessed by RP-HPLC-DAD, outlining benzoic acid and rutin as major
constituents in P. sempervivum and rutin and hesperidin in R. scutatus. Moreover, further biological
effects were tested on key enzymes involved in diabetes mellitus, Alzheimer’s disease and skin melano genesis revealing an important tyrosinase inhibitory effect of Pseudosempervivum water extract. More over, both species possessed antimicrobial properties towards bacteria and fungi relevant to public
health. Accordingly, we find that R. scutatus and P. sempervivum can be considered as novel functional
foods because they are rich sources of biologically active compounds that provide health benefits.
PB  - Royal Society of Chemistry
T2  - Food and Function
T1  - Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits
IS  - 7
VL  - 7
DO  - 10.1039/c6fo00695g
SP  - 3252
EP  - 3262
ER  - 

@article{
author = "Savran, Ahmet and Zengin, Gokhan and Aktumsek, Abdurrahman and Mocan, Andrei and Glamočlija, Jasmina and Ćirić, Ana and Soković, Marina",
year = "2016",
abstract = "The present study outlines a chemical characterization and further effects beneficial to health of edible
Rumex scutatus and Pseudosempervivum sempervivum, in addition to presenting the antioxidant,
enzyme inhibitory effects and antimicrobial properties of different extracts. The phenolic compounds
composition of the extracts was assessed by RP-HPLC-DAD, outlining benzoic acid and rutin as major
constituents in P. sempervivum and rutin and hesperidin in R. scutatus. Moreover, further biological
effects were tested on key enzymes involved in diabetes mellitus, Alzheimer’s disease and skin melano genesis revealing an important tyrosinase inhibitory effect of Pseudosempervivum water extract. More over, both species possessed antimicrobial properties towards bacteria and fungi relevant to public
health. Accordingly, we find that R. scutatus and P. sempervivum can be considered as novel functional
foods because they are rich sources of biologically active compounds that provide health benefits.",
publisher = "Royal Society of Chemistry",
journal = "Food and Function",
title = "Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits",
number = "7",
volume = "7",
doi = "10.1039/c6fo00695g",
pages = "3252-3262"
}

Savran, A., Zengin, G., Aktumsek, A., Mocan, A., Glamočlija, J., Ćirić, A.,& Soković, M.. (2016). Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits. in Food and Function
Royal Society of Chemistry., 7(7), 3252-3262.
https://doi.org/10.1039/c6fo00695g

Savran A, Zengin G, Aktumsek A, Mocan A, Glamočlija J, Ćirić A, Soković M. Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits. in Food and Function. 2016;7(7):3252-3262.
doi:10.1039/c6fo00695g .

Savran, Ahmet, Zengin, Gokhan, Aktumsek, Abdurrahman, Mocan, Andrei, Glamočlija, Jasmina, Ćirić, Ana, Soković, Marina, "Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits" in Food and Function, 7, no. 7 (2016):3252-3262,
https://doi.org/10.1039/c6fo00695g . .

TY  - JOUR
AU  - Petrović, Nada V.
AU  - Petrović, Slobodan S.
AU  - Džamić, Ana M.
AU  - Ćirić, Ana
AU  - Ristić, Mihailo S.
AU  - Milovanović, Stoja L.
AU  - Petrović, Slobodan D.
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6743
AB  - The chemical composition, antimicrobial and antioxidant activity of Thymus praecox extracts obtained by
fractional supercritical fluid extraction (SFE) with carbon dioxide were investigated. Properties of super critical extracts and extracts obtained by Soxhlet extraction were compared. The chemical composition
of extracts was determined using GC–FID and GC–MS analytical methods. The antimicrobial activity of
extracts was evaluated against 16 pathogenic microorganisms using microdilution method. Antioxidant
potential of extracts to neutralize DPPH radicals was also investigated. Investigated supercritical extracts
showed better antimicrobial activity than commercial antibiotics (streptomycin and ampicillin) and com mercial fungicides (bifonazol and ketoconazole) against all tested strains. For bacteria MIC and MBC were
38–200  g/ml and 75–300  g/ml, respectively, while for fungi MIC and MFC were 17–150  g/ml and
35–300  g/ml, respectively.
PB  - Elsevier B.V
T2  - The Journal of Supercritical Fluids
T1  - Chemical composition, antioxidant and antimicrobial activity of Thymus praecox supercritical extracts
VL  - 110
DO  - 10.1016/j.supflu.2016.01.001
SP  - 117
EP  - 125
ER  - 

@article{
author = "Petrović, Nada V. and Petrović, Slobodan S. and Džamić, Ana M. and Ćirić, Ana and Ristić, Mihailo S. and Milovanović, Stoja L. and Petrović, Slobodan D.",
year = "2016",
abstract = "The chemical composition, antimicrobial and antioxidant activity of Thymus praecox extracts obtained by
fractional supercritical fluid extraction (SFE) with carbon dioxide were investigated. Properties of super critical extracts and extracts obtained by Soxhlet extraction were compared. The chemical composition
of extracts was determined using GC–FID and GC–MS analytical methods. The antimicrobial activity of
extracts was evaluated against 16 pathogenic microorganisms using microdilution method. Antioxidant
potential of extracts to neutralize DPPH radicals was also investigated. Investigated supercritical extracts
showed better antimicrobial activity than commercial antibiotics (streptomycin and ampicillin) and com mercial fungicides (bifonazol and ketoconazole) against all tested strains. For bacteria MIC and MBC were
38–200  g/ml and 75–300  g/ml, respectively, while for fungi MIC and MFC were 17–150  g/ml and
35–300  g/ml, respectively.",
publisher = "Elsevier B.V",
journal = "The Journal of Supercritical Fluids",
title = "Chemical composition, antioxidant and antimicrobial activity of Thymus praecox supercritical extracts",
volume = "110",
doi = "10.1016/j.supflu.2016.01.001",
pages = "117-125"
}

Petrović, N. V., Petrović, S. S., Džamić, A. M., Ćirić, A., Ristić, M. S., Milovanović, S. L.,& Petrović, S. D.. (2016). Chemical composition, antioxidant and antimicrobial activity of Thymus praecox supercritical extracts. in The Journal of Supercritical Fluids
Elsevier B.V., 110, 117-125.
https://doi.org/10.1016/j.supflu.2016.01.001

Petrović NV, Petrović SS, Džamić AM, Ćirić A, Ristić MS, Milovanović SL, Petrović SD. Chemical composition, antioxidant and antimicrobial activity of Thymus praecox supercritical extracts. in The Journal of Supercritical Fluids. 2016;110:117-125.
doi:10.1016/j.supflu.2016.01.001 .

Petrović, Nada V., Petrović, Slobodan S., Džamić, Ana M., Ćirić, Ana, Ristić, Mihailo S., Milovanović, Stoja L., Petrović, Slobodan D., "Chemical composition, antioxidant and antimicrobial activity of Thymus praecox supercritical extracts" in The Journal of Supercritical Fluids, 110 (2016):117-125,
https://doi.org/10.1016/j.supflu.2016.01.001 . .

TY  - JOUR
AU  - Ušjak, Ljuboš J
AU  - Petrović, Silvana D
AU  - Drobac, Milica M
AU  - Soković, Marina
AU  - Stanojković, Tatjana P
AU  - Ćirić, Ana
AU  - Grozdanić, Nađa Ð
AU  - Niketić, Marjan S
PY  - 2016
UR  - http://onlinelibrary.wiley.com/doi/10.1002/cbdv.201500151/abstract
AB  - In this work, the chemical composition, antimicrobial and cytotoxic activity of Heracleum verticillatum Pančić and H. ternatum Velen. root, leaf, and fruit essential oils were investigated. The composition was analyzed by GC and GC/MS. Heracleum verticillatum and H. ternatum root oils were dominated by monoterpenes, mostly β-pinene (23.5% and 47.3%, respectively). Heracleum verticillatum leaf oil was characterized by monoterpenes, mainly limonene (20.3%), and sesquiterpenes, mostly (E)-caryophyllene (19.1%), while H. ternatum leaf oil by the high percentage of phenylpropanoids, with (Z)-isoelemicin (35.1%) being dominant constituent. Both fruit oils contained the majority of aliphatic esters, mostly octyl acetate (42.3% in H. verticillatum oil and 49.0% in H. ternatum oil). The antimicrobial activity of the oils was determined by microdilution method against eight bacterial and eight fungal strains. The strongest effect was exhibited by H. verticillatum root oil, particularly against Staphylococcus aureus, Salmonella typhimurium (MICs = 0.14 mg/ml, MBCs = 0.28 mg/ml), and Trichoderma viride (MIC = 0.05 mg/ml, MFC = 0.11 mg/ml). Cytotoxic effect was determined by MTT test against malignant HeLa, LS174, and A549 cells (IC50 = 5.9 - 146.0 μg/ml), and against normal MRC-5 cells (IC50 > 120.1 μg/ml). The best effect was exhibited by H. verticillatum root oil on A549 cells (IC50 = 5.9 μg/ml), and H. ternatum root oil against LS174 cells (IC50 = 6.7 μg/ml).
PB  - Wiley-VCH Verlag
T2  - Chemistry & Biodiversity
T1  - Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils.
IS  - 4
VL  - 13
DO  - 10.1002/cbdv.201500151
SP  - 466
EP  - 76
ER  - 

@article{
author = "Ušjak, Ljuboš J and Petrović, Silvana D and Drobac, Milica M and Soković, Marina and Stanojković, Tatjana P and Ćirić, Ana and Grozdanić, Nađa Ð and Niketić, Marjan S",
year = "2016",
abstract = "In this work, the chemical composition, antimicrobial and cytotoxic activity of Heracleum verticillatum Pančić and H. ternatum Velen. root, leaf, and fruit essential oils were investigated. The composition was analyzed by GC and GC/MS. Heracleum verticillatum and H. ternatum root oils were dominated by monoterpenes, mostly β-pinene (23.5% and 47.3%, respectively). Heracleum verticillatum leaf oil was characterized by monoterpenes, mainly limonene (20.3%), and sesquiterpenes, mostly (E)-caryophyllene (19.1%), while H. ternatum leaf oil by the high percentage of phenylpropanoids, with (Z)-isoelemicin (35.1%) being dominant constituent. Both fruit oils contained the majority of aliphatic esters, mostly octyl acetate (42.3% in H. verticillatum oil and 49.0% in H. ternatum oil). The antimicrobial activity of the oils was determined by microdilution method against eight bacterial and eight fungal strains. The strongest effect was exhibited by H. verticillatum root oil, particularly against Staphylococcus aureus, Salmonella typhimurium (MICs = 0.14 mg/ml, MBCs = 0.28 mg/ml), and Trichoderma viride (MIC = 0.05 mg/ml, MFC = 0.11 mg/ml). Cytotoxic effect was determined by MTT test against malignant HeLa, LS174, and A549 cells (IC50 = 5.9 - 146.0 μg/ml), and against normal MRC-5 cells (IC50 > 120.1 μg/ml). The best effect was exhibited by H. verticillatum root oil on A549 cells (IC50 = 5.9 μg/ml), and H. ternatum root oil against LS174 cells (IC50 = 6.7 μg/ml).",
publisher = "Wiley-VCH Verlag",
journal = "Chemistry & Biodiversity",
title = "Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils.",
number = "4",
volume = "13",
doi = "10.1002/cbdv.201500151",
pages = "466-76"
}

Ušjak, L. J., Petrović, S. D., Drobac, M. M., Soković, M., Stanojković, T. P., Ćirić, A., Grozdanić, N. Ð.,& Niketić, M. S.. (2016). Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils.. in Chemistry & Biodiversity
Wiley-VCH Verlag., 13(4), 466-76.
https://doi.org/10.1002/cbdv.201500151

Ušjak LJ, Petrović SD, Drobac MM, Soković M, Stanojković TP, Ćirić A, Grozdanić NÐ, Niketić MS. Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils.. in Chemistry & Biodiversity. 2016;13(4):466-76.
doi:10.1002/cbdv.201500151 .

Ušjak, Ljuboš J, Petrović, Silvana D, Drobac, Milica M, Soković, Marina, Stanojković, Tatjana P, Ćirić, Ana, Grozdanić, Nađa Ð, Niketić, Marjan S, "Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils." in Chemistry & Biodiversity, 13, no. 4 (2016):466-76,
https://doi.org/10.1002/cbdv.201500151 . .

TY  - JOUR
AU  - Usjak, Ljubos
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana
AU  - Niketić, Marjan
PY  - 2016
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27396211
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2990
AB  - The objective of this research was to analyze the chemical composition, antimicrobial and cytotoxic activity of Heracleun pyrenaicum subsp. pollinianum (Bertol.) F. Pedrotti & Pignatti (HPP) and H. orphanidis Boiss. (HO) essential oils. The composition of the oils was analyzed by GC and GC-MS. β-Pinene (35.1%) was the most abundant compound in HPP root oil, while (Z)-falcarinol (80.0%) dominated in HO root oil. (E)-Nerolidol (28.5%) was the main constituent in HPP leaf oil. HPP fruit oil, as well as HO leaf and fruit oils mainly contained aliphatic esters, mostly octyl acetate (50.5-84.5%). Antimicrobial screening was performed by microdilution method against eight bacterial and eight fungal strains. The strongest antibacterial activity was shown by both root oils (MICs 0.02-0.60 mg/mL and MBCs 0.04-2.50 mg/mL for HPP, and MICs 0.02-1.25 mg/mL and MBCs 0.04-2.50 mg/mL for HO), while the best antifungal potential was exhibited by HPP fruit oil (MICs 0.30-0.60 mg/mL and MFCs 0.60-1.25 mg/mL) and HO leaf oil (MICs 0.15-0.63 mg/mL and MFCs 0.30-1.25 mg/mL). The tested root and fruit oils exhibited strong cytotoxic effect, which was determined by MTT test against HeLa (IC50 7.53-21.07 µg/mL) and LS174 (IC50 24.16-58.86 µg/mL) cell lines.
T2  - Natural Product Communications
T1  - Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis.
IS  - 4
VL  - 11
SP  - 529
EP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2990
ER  - 

@article{
author = "Usjak, Ljubos and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana and Niketić, Marjan",
year = "2016",
abstract = "The objective of this research was to analyze the chemical composition, antimicrobial and cytotoxic activity of Heracleun pyrenaicum subsp. pollinianum (Bertol.) F. Pedrotti & Pignatti (HPP) and H. orphanidis Boiss. (HO) essential oils. The composition of the oils was analyzed by GC and GC-MS. β-Pinene (35.1%) was the most abundant compound in HPP root oil, while (Z)-falcarinol (80.0%) dominated in HO root oil. (E)-Nerolidol (28.5%) was the main constituent in HPP leaf oil. HPP fruit oil, as well as HO leaf and fruit oils mainly contained aliphatic esters, mostly octyl acetate (50.5-84.5%). Antimicrobial screening was performed by microdilution method against eight bacterial and eight fungal strains. The strongest antibacterial activity was shown by both root oils (MICs 0.02-0.60 mg/mL and MBCs 0.04-2.50 mg/mL for HPP, and MICs 0.02-1.25 mg/mL and MBCs 0.04-2.50 mg/mL for HO), while the best antifungal potential was exhibited by HPP fruit oil (MICs 0.30-0.60 mg/mL and MFCs 0.60-1.25 mg/mL) and HO leaf oil (MICs 0.15-0.63 mg/mL and MFCs 0.30-1.25 mg/mL). The tested root and fruit oils exhibited strong cytotoxic effect, which was determined by MTT test against HeLa (IC50 7.53-21.07 µg/mL) and LS174 (IC50 24.16-58.86 µg/mL) cell lines.",
journal = "Natural Product Communications",
title = "Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis.",
number = "4",
volume = "11",
pages = "529-34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2990"
}

Usjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A.,& Niketić, M.. (2016). Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis.. in Natural Product Communications, 11(4), 529-34.
https://hdl.handle.net/21.15107/rcub_ibiss_2990

Usjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić A, Niketić M. Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis.. in Natural Product Communications. 2016;11(4):529-34.
https://hdl.handle.net/21.15107/rcub_ibiss_2990 .

Usjak, Ljubos, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana, Niketić, Marjan, "Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis." in Natural Product Communications, 11, no. 4 (2016):529-34,
https://hdl.handle.net/21.15107/rcub_ibiss_2990 .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6200
AB  - The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.
PB  - Berlin Heidelberg : Springer-Verlag
T2  - Planta
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)
IS  - 2
VL  - 244
DO  - 10.1007/s00425-016-2513-7
SP  - 393
EP  - 404
ER  - 

@article{
author = "Stojičić, Dragana and Tošić, Svetlana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Janošević, Dušica and Uzelac, Branka",
year = "2016",
abstract = "The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.",
publisher = "Berlin Heidelberg : Springer-Verlag",
journal = "Planta",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)",
number = "2",
volume = "244",
doi = "10.1007/s00425-016-2513-7",
pages = "393-404"
}

Stojičić, D., Tošić, S., Slavkovska, V., Zlatković, B., Budimir, S., Janošević, D.,& Uzelac, B.. (2016). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta
Berlin Heidelberg : Springer-Verlag., 244(2), 393-404.
https://doi.org/10.1007/s00425-016-2513-7

Stojičić D, Tošić S, Slavkovska V, Zlatković B, Budimir S, Janošević D, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta. 2016;244(2):393-404.
doi:10.1007/s00425-016-2513-7 .

Stojičić, Dragana, Tošić, Svetlana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Janošević, Dušica, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)" in Planta, 244, no. 2 (2016):393-404,
https://doi.org/10.1007/s00425-016-2513-7 . .

TY  - JOUR
AU  - Marković, Milena
AU  - Ristić, Mihailo
AU  - Popović, Zorica
AU  - Matić, Rada
AU  - Nikolić, Biljana
AU  - Vidaković, Vera
AU  - Obratov-Petković, Dragica
AU  - Bojović, Srđan
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3502
AB  - The composition of the essential oil of the twigs and needles of Taxus baccata L. from three natural populations in Serbia has been determined by GC/MS analysis. Of the 91 detected compounds, 87 were identified. The most abundant compound classes were aliphatic alcohols, terpenes, aliphatic hydrocarbons, and aliphatic aldehydes, which together comprised ca. 86.92% of the total oil composition. The dominant constituents were oct‐1‐en‐3‐ol (23.48%), (3Z)‐hex‐3‐en‐1‐ol (11.46%; aliphatic alcohols), and myrtenol (11.38%; oxygenated monoterpene). The PCA of 22 selected compounds revealed differentiations of populations based on geographic distribution. The CA showed that Populations I and II from the Dinaric Alps were similar, and that Population III from the Balkan mountain system was distinct. This was the first investigation of interpopulation variability of T. baccata populations based on essential oil composition. The results of this study were compared with those of previous studies concerning volatile compounds produced by Taxus species. The results indicate that the essential oil content of T. baccata populations from this study is unique, mostly resembling the population from southeast Serbia.
PB  - Zürich: Wiley‐VHCA AG
T2  - Chemistry and Biodiversity
T1  - Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia
IS  - 7
VL  - 13
DO  - 10.1002/cbdv.201500326
SP  - 943
EP  - 953
ER  - 

@article{
author = "Marković, Milena and Ristić, Mihailo and Popović, Zorica and Matić, Rada and Nikolić, Biljana and Vidaković, Vera and Obratov-Petković, Dragica and Bojović, Srđan",
year = "2016",
abstract = "The composition of the essential oil of the twigs and needles of Taxus baccata L. from three natural populations in Serbia has been determined by GC/MS analysis. Of the 91 detected compounds, 87 were identified. The most abundant compound classes were aliphatic alcohols, terpenes, aliphatic hydrocarbons, and aliphatic aldehydes, which together comprised ca. 86.92% of the total oil composition. The dominant constituents were oct‐1‐en‐3‐ol (23.48%), (3Z)‐hex‐3‐en‐1‐ol (11.46%; aliphatic alcohols), and myrtenol (11.38%; oxygenated monoterpene). The PCA of 22 selected compounds revealed differentiations of populations based on geographic distribution. The CA showed that Populations I and II from the Dinaric Alps were similar, and that Population III from the Balkan mountain system was distinct. This was the first investigation of interpopulation variability of T. baccata populations based on essential oil composition. The results of this study were compared with those of previous studies concerning volatile compounds produced by Taxus species. The results indicate that the essential oil content of T. baccata populations from this study is unique, mostly resembling the population from southeast Serbia.",
publisher = "Zürich: Wiley‐VHCA AG",
journal = "Chemistry and Biodiversity",
title = "Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia",
number = "7",
volume = "13",
doi = "10.1002/cbdv.201500326",
pages = "943-953"
}

Marković, M., Ristić, M., Popović, Z., Matić, R., Nikolić, B., Vidaković, V., Obratov-Petković, D.,& Bojović, S.. (2016). Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia. in Chemistry and Biodiversity
Zürich: Wiley‐VHCA AG., 13(7), 943-953.
https://doi.org/10.1002/cbdv.201500326

Marković M, Ristić M, Popović Z, Matić R, Nikolić B, Vidaković V, Obratov-Petković D, Bojović S. Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia. in Chemistry and Biodiversity. 2016;13(7):943-953.
doi:10.1002/cbdv.201500326 .

Marković, Milena, Ristić, Mihailo, Popović, Zorica, Matić, Rada, Nikolić, Biljana, Vidaković, Vera, Obratov-Petković, Dragica, Bojović, Srđan, "Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia" in Chemistry and Biodiversity, 13, no. 7 (2016):943-953,
https://doi.org/10.1002/cbdv.201500326 . .

TY  - CONF
AU  - Marković, Marija
AU  - Skorić, Marijana
AU  - Živković, Suzana
AU  - Jadranin, Milka
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Gligorijević, Nevenka
AU  - Janković, Radmila
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Todorović, Slađana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6184
AB  - Inula oculus-christi L. is a perennial species distributed in Asia, Central Europe and the Balkan Peninsula. In this study a potential cytotoxic and antimicrobial activity of ethanol extracts obtained from different parts of I. oculus-christi plants was investigated. Cytotoxic activity of extracts was determined on three tumor cell lines (HeLa, FemX and LS-174) by MTT assay. The extracts of leaves and flowers showed significant cytotoxic activity against all investigated cell lines, while rhizome, root and trunk extracts had no cytotoxic activity. The IC50 values for flower extract were 68.70 ± 2.79 μg mL-1 for HeLa cells, 88.91 ± 1.66 μg mL-1 for FemX cells and 81.46 ± 8.45 μg mL-1 for LS-174 cells, whereas the IC50 values for leaf extract were 73.69 ± 5.35 μg mL-1 for He-La cells, 71.58 ± 4.25 μg mL-1 for FemX cells and 96.37 ± 4.92 μg mL-1 for LS-174 cells. The observed cytotoxic activity could be attributed to several bioactive sesquiterpenoids and flavonoids which were more abundant in leaf and flower extracts according to LC-DAD/MS non-targeted screening. In the antimicrobial bioassays against eight bacteria and microfungi, all tested extracts exhibited higher antimicrobial activity in comparison with commercial antimicrobial agents used as control. These results indicate the possibility of application of I. oculus-christi extracts as valuable natural products in medicine, agronomy and food industry.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6184
ER  - 

@conference{
author = "Marković, Marija and Skorić, Marijana and Živković, Suzana and Jadranin, Milka and Dragićević, Milan and Tešević, Vele and Gligorijević, Nevenka and Janković, Radmila and Glamočlija, Jasmina and Soković, Marina and Todorović, Slađana",
year = "2015",
abstract = "Inula oculus-christi L. is a perennial species distributed in Asia, Central Europe and the Balkan Peninsula. In this study a potential cytotoxic and antimicrobial activity of ethanol extracts obtained from different parts of I. oculus-christi plants was investigated. Cytotoxic activity of extracts was determined on three tumor cell lines (HeLa, FemX and LS-174) by MTT assay. The extracts of leaves and flowers showed significant cytotoxic activity against all investigated cell lines, while rhizome, root and trunk extracts had no cytotoxic activity. The IC50 values for flower extract were 68.70 ± 2.79 μg mL-1 for HeLa cells, 88.91 ± 1.66 μg mL-1 for FemX cells and 81.46 ± 8.45 μg mL-1 for LS-174 cells, whereas the IC50 values for leaf extract were 73.69 ± 5.35 μg mL-1 for He-La cells, 71.58 ± 4.25 μg mL-1 for FemX cells and 96.37 ± 4.92 μg mL-1 for LS-174 cells. The observed cytotoxic activity could be attributed to several bioactive sesquiterpenoids and flavonoids which were more abundant in leaf and flower extracts according to LC-DAD/MS non-targeted screening. In the antimicrobial bioassays against eight bacteria and microfungi, all tested extracts exhibited higher antimicrobial activity in comparison with commercial antimicrobial agents used as control. These results indicate the possibility of application of I. oculus-christi extracts as valuable natural products in medicine, agronomy and food industry.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6184"
}

Marković, M., Skorić, M., Živković, S., Jadranin, M., Dragićević, M., Tešević, V., Gligorijević, N., Janković, R., Glamočlija, J., Soković, M.,& Todorović, S.. (2015). Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 85.
https://hdl.handle.net/21.15107/rcub_ibiss_6184

Marković M, Skorić M, Živković S, Jadranin M, Dragićević M, Tešević V, Gligorijević N, Janković R, Glamočlija J, Soković M, Todorović S. Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:85.
https://hdl.handle.net/21.15107/rcub_ibiss_6184 .

Marković, Marija, Skorić, Marijana, Živković, Suzana, Jadranin, Milka, Dragićević, Milan, Tešević, Vele, Gligorijević, Nevenka, Janković, Radmila, Glamočlija, Jasmina, Soković, Marina, Todorović, Slađana, "Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):85,
https://hdl.handle.net/21.15107/rcub_ibiss_6184 .

TY  - JOUR
AU  - Mileski, Ksenija
AU  - Džamić, Ana M.
AU  - Ćirić, Ana
AU  - Grujić, Slavica
AU  - Ristić, M.
AU  - Matevski, V.
AU  - Marin, Petar D.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2304
AB  - This study was undertaken to determine the antioxidant and antimicrobial
   effect of essential oil and extracts of Echinophora sibthorpiana Guss.
   (fam. Apiaceae) collected in Macedonia. The chemical composition of E.
   sibthorpiana essential oil was characterized by the presence of methyl
   eugenol (60.40\%), p-cymene (11.18\%) and a-phellandrene (10.23\%). The
   free radical scavenging activity of extracts and essential oil was
   evaluated by DPPH and ABTS assays. The aqueous extract of aerial parts
   exhibited the strongest scavenging activity (IC50=1.67 mg/ml); results
   of the ABTS test showed that the most effective was the ethanol extract
   of aerial parts (1.11 mg vit. C/g). The essential oil showed stronger
   antioxidant activity compared to hydroxyanisole, ascorbic acid and
   quercetin that were used in the DPPH and ABTS tests, respectively. The
   total phenolic and flavonoid concentrations in the extracts ranged
   between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g, respectively. The
   antimicrobial properties of the extracts and essential oil were
   investigated using a micro-well dilution technique against human
   pathogenic strains. The results were comparable with the effects of the
   positive controls, streptomycin and fluconazole. These findings indicate
   that E. sibthorpiana extracts and oil can be used in preventive
   treatments and as an alternative for synthetic preservatives.
T2  - Archives of Biological Sciences
T1  - RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL AND
 EXTRACTS OF ECHINOPHORA SIBTHORPIANA GUSS. FROM MACEDONIA
IS  - 1
VL  - 66
DO  - 10.2298/ABS1401401M
SP  - 401
EP  - 413
ER  - 

@article{
author = "Mileski, Ksenija and Džamić, Ana M. and Ćirić, Ana and Grujić, Slavica and Ristić, M. and Matevski, V. and Marin, Petar D.",
year = "2014",
abstract = "This study was undertaken to determine the antioxidant and antimicrobial
   effect of essential oil and extracts of Echinophora sibthorpiana Guss.
   (fam. Apiaceae) collected in Macedonia. The chemical composition of E.
   sibthorpiana essential oil was characterized by the presence of methyl
   eugenol (60.40\%), p-cymene (11.18\%) and a-phellandrene (10.23\%). The
   free radical scavenging activity of extracts and essential oil was
   evaluated by DPPH and ABTS assays. The aqueous extract of aerial parts
   exhibited the strongest scavenging activity (IC50=1.67 mg/ml); results
   of the ABTS test showed that the most effective was the ethanol extract
   of aerial parts (1.11 mg vit. C/g). The essential oil showed stronger
   antioxidant activity compared to hydroxyanisole, ascorbic acid and
   quercetin that were used in the DPPH and ABTS tests, respectively. The
   total phenolic and flavonoid concentrations in the extracts ranged
   between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g, respectively. The
   antimicrobial properties of the extracts and essential oil were
   investigated using a micro-well dilution technique against human
   pathogenic strains. The results were comparable with the effects of the
   positive controls, streptomycin and fluconazole. These findings indicate
   that E. sibthorpiana extracts and oil can be used in preventive
   treatments and as an alternative for synthetic preservatives.",
journal = "Archives of Biological Sciences",
title = "RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL AND
 EXTRACTS OF ECHINOPHORA SIBTHORPIANA GUSS. FROM MACEDONIA",
number = "1",
volume = "66",
doi = "10.2298/ABS1401401M",
pages = "401-413"
}

Mileski, K., Džamić, A. M., Ćirić, A., Grujić, S., Ristić, M., Matevski, V.,& Marin, P. D.. (2014). RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL AND
 EXTRACTS OF ECHINOPHORA SIBTHORPIANA GUSS. FROM MACEDONIA. in Archives of Biological Sciences, 66(1), 401-413.
https://doi.org/10.2298/ABS1401401M

Mileski K, Džamić AM, Ćirić A, Grujić S, Ristić M, Matevski V, Marin PD. RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL AND
 EXTRACTS OF ECHINOPHORA SIBTHORPIANA GUSS. FROM MACEDONIA. in Archives of Biological Sciences. 2014;66(1):401-413.
doi:10.2298/ABS1401401M .

Mileski, Ksenija, Džamić, Ana M., Ćirić, Ana, Grujić, Slavica, Ristić, M., Matevski, V., Marin, Petar D., "RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL AND
 EXTRACTS OF ECHINOPHORA SIBTHORPIANA GUSS. FROM MACEDONIA" in Archives of Biological Sciences, 66, no. 1 (2014):401-413,
https://doi.org/10.2298/ABS1401401M . .

TY  - CONF
AU  - Skorić, Marijana
AU  - Todorović, Slađana
AU  - Ristić, Mihailo
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Živković, Suzana
AU  - Stojić, Andreja
AU  - Puač, Nevena
AU  - Kanellis, Angelos K
PY  - 2013
UR  - http://terpnet2013.pharm.auth.gr/site/TERPNET_BOOK_OF_ABSTRACTS.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6320
AB  - Cistus creticus subsp. creticus is a native plant of the Mediterranean region and it has been used since ancient times for its medicinal properties. Labdane type diterpenes are predominant compounds in leaves and stems of the C. creticus subsp. creticus, as well as in resin, while other terpenes, polyphenols and flavonoids contribute to chemical profile of this species. In vitro plant tissue culture is an attractive alternative approach to the traditional methods of plantations, as it offers a controlled supply of biochemicals independent of plant availability and more consistent product quality. In order to obtain applicable production of secondary metabolites of interest we have implemented in vitro culture of several clones of C. creticus, which were previously morphogenetically described. The ethanol extracts of obtained in vitro cultures were analyzed by GC-FID and GC-MS, and were characterized concerning the total phenolic and flavonoid contents. Labdane diterpenes were the most abundant compounds in above ground parts extracts, but absent from the root extracts. Head-space GC-MS was used to determine the profile of volatile compounds in the atmosphere of the culture vessels. α pinene, β pinene, and camphene were the most abundant ones. PTR-MS was used for further quantification of representative volatile compounds in the atmosphere of the culture vessels. Finally, the biological activity of obtained ethanol extract was studied. Microdilution method was used for investigation of antibacterial and antifungal activity. Almost all tested extract against human pathogenic bacteria showed better antibacterial activity than streptomycin, and much better antibacterial activity than ampicillin. All tested extracts also exhibited slightly higher or similar antifungal potential as bifonazole, and showed much better antifungal effect than ketoconazole. Antioxidant activity was determined by using DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, while cytotoxic activity was previously demonstrated by sulforhodamine B (SRB) assay.
PB  - Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki
C3  - TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece
T1  - In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds
SP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6320
ER  - 

@conference{
author = "Skorić, Marijana and Todorović, Slađana and Ristić, Mihailo and Soković, Marina and Glamočlija, Jasmina and Živković, Suzana and Stojić, Andreja and Puač, Nevena and Kanellis, Angelos K",
year = "2013",
abstract = "Cistus creticus subsp. creticus is a native plant of the Mediterranean region and it has been used since ancient times for its medicinal properties. Labdane type diterpenes are predominant compounds in leaves and stems of the C. creticus subsp. creticus, as well as in resin, while other terpenes, polyphenols and flavonoids contribute to chemical profile of this species. In vitro plant tissue culture is an attractive alternative approach to the traditional methods of plantations, as it offers a controlled supply of biochemicals independent of plant availability and more consistent product quality. In order to obtain applicable production of secondary metabolites of interest we have implemented in vitro culture of several clones of C. creticus, which were previously morphogenetically described. The ethanol extracts of obtained in vitro cultures were analyzed by GC-FID and GC-MS, and were characterized concerning the total phenolic and flavonoid contents. Labdane diterpenes were the most abundant compounds in above ground parts extracts, but absent from the root extracts. Head-space GC-MS was used to determine the profile of volatile compounds in the atmosphere of the culture vessels. α pinene, β pinene, and camphene were the most abundant ones. PTR-MS was used for further quantification of representative volatile compounds in the atmosphere of the culture vessels. Finally, the biological activity of obtained ethanol extract was studied. Microdilution method was used for investigation of antibacterial and antifungal activity. Almost all tested extract against human pathogenic bacteria showed better antibacterial activity than streptomycin, and much better antibacterial activity than ampicillin. All tested extracts also exhibited slightly higher or similar antifungal potential as bifonazole, and showed much better antifungal effect than ketoconazole. Antioxidant activity was determined by using DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, while cytotoxic activity was previously demonstrated by sulforhodamine B (SRB) assay.",
publisher = "Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki",
journal = "TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece",
title = "In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds",
pages = "132",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6320"
}

Skorić, M., Todorović, S., Ristić, M., Soković, M., Glamočlija, J., Živković, S., Stojić, A., Puač, N.,& Kanellis, A. K.. (2013). In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds. in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece
Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki., 132.
https://hdl.handle.net/21.15107/rcub_ibiss_6320

Skorić M, Todorović S, Ristić M, Soković M, Glamočlija J, Živković S, Stojić A, Puač N, Kanellis AK. In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds. in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece. 2013;:132.
https://hdl.handle.net/21.15107/rcub_ibiss_6320 .

Skorić, Marijana, Todorović, Slađana, Ristić, Mihailo, Soković, Marina, Glamočlija, Jasmina, Živković, Suzana, Stojić, Andreja, Puač, Nevena, Kanellis, Angelos K, "In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds" in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece (2013):132,
https://hdl.handle.net/21.15107/rcub_ibiss_6320 .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Kukić-Marković, Jelena M
AU  - Petrović, Silvana D
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/963
AB  - Background Three pure compounds that naturally occur in plants were of particular interest to our study regarding the possibility of using them as food preservatives: p-coumaric acid (found in peanuts, tomatoes, carrots, garlic, wine, vinegar, etc.), caffeic acid (found in argan oil, oats, wheat, rice and olive oil) and rutin (found in asparagus, citrus fruits, berries, apple, apricot, asparagus, beef and beer). In the following study we investigated in situ antioxidant and antimicrobial activities of three pure compounds, namely caffeic acid, p-coumaric acid and rutin, naturally occurring in plants. Results Two food systems were used in order to obtain information on how these compounds react in actual food systems rather than microbiological media. The results indicated good antioxidant activity in in situ food systems. For tested phenolic compounds it was further shown that they successively inhibited the development of the isolated food contaminant Staphylococcus aureus in chicken soup. Panelist found that organoleptic characteristics of chicken soup and pork meat improved after treatment with phenolics. Conclusion Our findings alone, along with the potential use of phenolic compounds that are widespread in nature, may imply their potential use as preservatives in the food industry. (c) 2013 Society of Chemical Industry
T2  - Journal of the Science of Food and Agriculture
T1  - In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems
IS  - 13
VL  - 93
DO  - 10.1002/jsfa.6156
SP  - 197
EP  - 3208
ER  - 

@article{
author = "Stojković, Dejan and Petrović, Jovana and Soković, Marina and Glamočlija, Jasmina and Kukić-Marković, Jelena M and Petrović, Silvana D",
year = "2013",
abstract = "Background Three pure compounds that naturally occur in plants were of particular interest to our study regarding the possibility of using them as food preservatives: p-coumaric acid (found in peanuts, tomatoes, carrots, garlic, wine, vinegar, etc.), caffeic acid (found in argan oil, oats, wheat, rice and olive oil) and rutin (found in asparagus, citrus fruits, berries, apple, apricot, asparagus, beef and beer). In the following study we investigated in situ antioxidant and antimicrobial activities of three pure compounds, namely caffeic acid, p-coumaric acid and rutin, naturally occurring in plants. Results Two food systems were used in order to obtain information on how these compounds react in actual food systems rather than microbiological media. The results indicated good antioxidant activity in in situ food systems. For tested phenolic compounds it was further shown that they successively inhibited the development of the isolated food contaminant Staphylococcus aureus in chicken soup. Panelist found that organoleptic characteristics of chicken soup and pork meat improved after treatment with phenolics. Conclusion Our findings alone, along with the potential use of phenolic compounds that are widespread in nature, may imply their potential use as preservatives in the food industry. (c) 2013 Society of Chemical Industry",
journal = "Journal of the Science of Food and Agriculture",
title = "In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems",
number = "13",
volume = "93",
doi = "10.1002/jsfa.6156",
pages = "197-3208"
}

Stojković, D., Petrović, J., Soković, M., Glamočlija, J., Kukić-Marković, J. M.,& Petrović, S. D.. (2013). In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems. in Journal of the Science of Food and Agriculture, 93(13), 197-3208.
https://doi.org/10.1002/jsfa.6156

Stojković D, Petrović J, Soković M, Glamočlija J, Kukić-Marković JM, Petrović SD. In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems. in Journal of the Science of Food and Agriculture. 2013;93(13):197-3208.
doi:10.1002/jsfa.6156 .

Stojković, Dejan, Petrović, Jovana, Soković, Marina, Glamočlija, Jasmina, Kukić-Marković, Jelena M, Petrović, Silvana D, "In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems" in Journal of the Science of Food and Agriculture, 93, no. 13 (2013):197-3208,
https://doi.org/10.1002/jsfa.6156 . .