TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6693
AB  - Pollution of surface waters still represents one of the major environmental challenges in the Republic of Serbia, where wastewaters are discharged directly into the recipients without proper treatment. Within the last decade, we have assessed the microbiological water quality of more than 100 sites situated at different water bodies in Serbia. Quality was determined using faecal indicator bacteria, while microbial source tracking was employed to assess the source of pollution. Almost 50 % of the investigated sites were characterized by critical or even higher level of faecal contamination indicating that untreated wastewaters indeed represent significant pollution pressure on surface waters. Human-associated markers were prevalent in samples from the majority of contaminated sites but the source of pollution was not exclusively human-associated. 
The impact on the water quality of the Danube River was demonstrated in our previous research conducted at the whole river level within the Joint Danube Surveys. In the river stretch from Novi Sad to its confluence with the Velika Morava River, all the midstream samples were critically polluted. In this section, the highest level of pollution was recorded downstream of Belgrade. As ultimate recipients of wastewaters, Danube and its largest tributary Sava currently represent the only solution for disposing of wastewaters originating from the Serbian capital‘s 1,700,000 inhabitants. Such kind of disposal rises additional issues such as antimicrobial resistance and presence of infectious agents in water. For instance during COVID-19 pandemic, we have demonstrated that SARS-CoV-2 RNA can be detected even in surface waters of the Danube River at the sites receiving high wastewater loads from Belgrade which was the unique case for the Basin. 
Despites its extreme importance in this case, wastewater-based epidemiology is neglected in our country and hereby we would like to emphasize the need for implementation of program of such kind in Serbia.
PB  - Belgrade: Serbian Society for Microbiology
C3  - Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia
T1  - Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6693
ER  - 

@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szanto-Egesz, Reka and Lukacs, Alena and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Ana and Vojnović-Milutinović, Danijela and Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2024",
abstract = "Pollution of surface waters still represents one of the major environmental challenges in the Republic of Serbia, where wastewaters are discharged directly into the recipients without proper treatment. Within the last decade, we have assessed the microbiological water quality of more than 100 sites situated at different water bodies in Serbia. Quality was determined using faecal indicator bacteria, while microbial source tracking was employed to assess the source of pollution. Almost 50 % of the investigated sites were characterized by critical or even higher level of faecal contamination indicating that untreated wastewaters indeed represent significant pollution pressure on surface waters. Human-associated markers were prevalent in samples from the majority of contaminated sites but the source of pollution was not exclusively human-associated. 
The impact on the water quality of the Danube River was demonstrated in our previous research conducted at the whole river level within the Joint Danube Surveys. In the river stretch from Novi Sad to its confluence with the Velika Morava River, all the midstream samples were critically polluted. In this section, the highest level of pollution was recorded downstream of Belgrade. As ultimate recipients of wastewaters, Danube and its largest tributary Sava currently represent the only solution for disposing of wastewaters originating from the Serbian capital‘s 1,700,000 inhabitants. Such kind of disposal rises additional issues such as antimicrobial resistance and presence of infectious agents in water. For instance during COVID-19 pandemic, we have demonstrated that SARS-CoV-2 RNA can be detected even in surface waters of the Danube River at the sites receiving high wastewater loads from Belgrade which was the unique case for the Basin. 
Despites its extreme importance in this case, wastewater-based epidemiology is neglected in our country and hereby we would like to emphasize the need for implementation of program of such kind in Serbia.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia",
title = "Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6693"
}

Kolarević, S., Micsinai, A., Szanto-Egesz, R., Lukacs, A., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, A., Vojnović-Milutinović, D., Kirschner, A., Farnleitner, A., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2024). Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia. in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia
Belgrade: Serbian Society for Microbiology., 65.
https://hdl.handle.net/21.15107/rcub_ibiss_6693

Kolarević S, Micsinai A, Szanto-Egesz R, Lukacs A, Kračun-Kolarević M, Jovanović Marić J, Đorđević A, Vojnović-Milutinović D, Kirschner A, Farnleitner A, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia. in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia. 2024;:65.
https://hdl.handle.net/21.15107/rcub_ibiss_6693 .

Kolarević, Stoimir, Micsinai, Adrienn, Szanto-Egesz, Reka, Lukacs, Alena, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Ana, Vojnović-Milutinović, Danijela, Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia" in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia (2024):65,
https://hdl.handle.net/21.15107/rcub_ibiss_6693 .

TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Ilić, Marija
AU  - Anđus, Stefan
AU  - Paunović, Momir
AU  - Kolarević, Stoimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6595
AB  - Analiza mikrobiološkog kvaliteta vode na više lokaliteta (reke Tip 4 i 6, veštačka vodna tela
– akumulacije) na teritoriji Specijalnog rezervata prirode „Uvac“ obuhvatila je određivanje
koncentracije ukupnih koliforma i Escherichia coli pomoću Colilert-18 sistema. U slučaju
reka uzimani su površinski uzorci vode (dubina 0,2 m), dok su u slučaju akumulacija uzeti
uzorci sa više dubina. Najveći pritisci fekalnog zagađenja zabeleženi su na lokalitetima
Uvac-Čedovo i Vapa-Čedovo (umeren ekološki status – III klasa). Na rekama Tip 6
zabeležena je I ili II klasa zavisno od datuma uzorkovanja.
AB  - Analysis of the microbiological water quality at sampling sites within the Special nature
reserve „Uvac“ included determination of total coliforms and Escherichia coli
concentration using the Colilert-18 system. Surface water samples (depth 0.2 m) were taken
for rivers, while for reservoirs samples were taken from several depths. The highest
pressures of faecal pollution were recorded at sites Uvac-Čedovo and Vapa-Čedovo
(moderate ecological status, III class). In the case of the remaining analysed sites, I or II
class was recorded depending on the sampling date.
PB  - Belgarde: Serbian Association for Water Protection
C3  - Conference proceedings: 52nd Annual Conference of the Serbian Water Pollution Control Society: Water 2023; 2023 May 31 - Jun 2; Palić, Serbia
T1  - Analiza mikrobioloških parametara kvaliteta vode na području specijalnog rezervata prirode UVAC
T1  - Analysis of microbiological parameters of water quality in the area of the special nature reserve "UVAC"
SP  - 57
EP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6595
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Ilić, Marija and Anđus, Stefan and Paunović, Momir and Kolarević, Stoimir",
year = "2023",
abstract = "Analiza mikrobiološkog kvaliteta vode na više lokaliteta (reke Tip 4 i 6, veštačka vodna tela
– akumulacije) na teritoriji Specijalnog rezervata prirode „Uvac“ obuhvatila je određivanje
koncentracije ukupnih koliforma i Escherichia coli pomoću Colilert-18 sistema. U slučaju
reka uzimani su površinski uzorci vode (dubina 0,2 m), dok su u slučaju akumulacija uzeti
uzorci sa više dubina. Najveći pritisci fekalnog zagađenja zabeleženi su na lokalitetima
Uvac-Čedovo i Vapa-Čedovo (umeren ekološki status – III klasa). Na rekama Tip 6
zabeležena je I ili II klasa zavisno od datuma uzorkovanja., Analysis of the microbiological water quality at sampling sites within the Special nature
reserve „Uvac“ included determination of total coliforms and Escherichia coli
concentration using the Colilert-18 system. Surface water samples (depth 0.2 m) were taken
for rivers, while for reservoirs samples were taken from several depths. The highest
pressures of faecal pollution were recorded at sites Uvac-Čedovo and Vapa-Čedovo
(moderate ecological status, III class). In the case of the remaining analysed sites, I or II
class was recorded depending on the sampling date.",
publisher = "Belgarde: Serbian Association for Water Protection",
journal = "Conference proceedings: 52nd Annual Conference of the Serbian Water Pollution Control Society: Water 2023; 2023 May 31 - Jun 2; Palić, Serbia",
title = "Analiza mikrobioloških parametara kvaliteta vode na području specijalnog rezervata prirode UVAC, Analysis of microbiological parameters of water quality in the area of the special nature reserve "UVAC"",
pages = "57-60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6595"
}

Kračun-Kolarević, M., Jovanović Marić, J., Ilić, M., Anđus, S., Paunović, M.,& Kolarević, S.. (2023). Analiza mikrobioloških parametara kvaliteta vode na području specijalnog rezervata prirode UVAC. in Conference proceedings: 52nd Annual Conference of the Serbian Water Pollution Control Society: Water 2023; 2023 May 31 - Jun 2; Palić, Serbia
Belgarde: Serbian Association for Water Protection., 57-60.
https://hdl.handle.net/21.15107/rcub_ibiss_6595

Kračun-Kolarević M, Jovanović Marić J, Ilić M, Anđus S, Paunović M, Kolarević S. Analiza mikrobioloških parametara kvaliteta vode na području specijalnog rezervata prirode UVAC. in Conference proceedings: 52nd Annual Conference of the Serbian Water Pollution Control Society: Water 2023; 2023 May 31 - Jun 2; Palić, Serbia. 2023;:57-60.
https://hdl.handle.net/21.15107/rcub_ibiss_6595 .

Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Ilić, Marija, Anđus, Stefan, Paunović, Momir, Kolarević, Stoimir, "Analiza mikrobioloških parametara kvaliteta vode na području specijalnog rezervata prirode UVAC" in Conference proceedings: 52nd Annual Conference of the Serbian Water Pollution Control Society: Water 2023; 2023 May 31 - Jun 2; Palić, Serbia (2023):57-60,
https://hdl.handle.net/21.15107/rcub_ibiss_6595 .

TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kolarević, Stoimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6592
AB  - Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6592
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kolarević, Stoimir",
year = "2023",
abstract = "Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6592"
}

Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kolarević, S.. (2023). In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592

Kračun-Kolarević M, Jovanović Marić J, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kolarević S. In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kolarević, Stoimir, "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):18,
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Adrienn, Micsinai
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6591
AB  - Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia
SP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6591
ER  - 

@conference{
author = "Kolarević, Stoimir and Adrienn, Micsinai and Szanto-Egesz, Reka and Lukacs, Alena and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Ana and Vojnović-Milutinović, Danijela and Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2023",
abstract = "Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia",
pages = "11",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6591"
}

Kolarević, S., Adrienn, M., Szanto-Egesz, R., Lukacs, A., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, A., Vojnović-Milutinović, D., Kirschner, A., Farnleitner, A., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2023). Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 11.
https://hdl.handle.net/21.15107/rcub_ibiss_6591

Kolarević S, Adrienn M, Szanto-Egesz R, Lukacs A, Kračun-Kolarević M, Jovanović Marić J, Đorđević A, Vojnović-Milutinović D, Kirschner A, Farnleitner A, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:11.
https://hdl.handle.net/21.15107/rcub_ibiss_6591 .

Kolarević, Stoimir, Adrienn, Micsinai, Szanto-Egesz, Reka, Lukacs, Alena, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Ana, Vojnović-Milutinović, Danijela, Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):11,
https://hdl.handle.net/21.15107/rcub_ibiss_6591 .

TY  - JOUR
AU  - Takodi, Nada
AU  - Drobac Backović, Damjana
AU  - Marinović, Zoran
AU  - Sunjog, Karolina
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Kitanović, Nevena
AU  - Vuković-Gačić, Branka
AU  - Lujić, Jelena
AU  - Svirčev, Zorica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6464
AB  - Lake Ludaš is a protected nature reserve in Serbia that has been the focus of scientific interest, due to
perpetual water quality deterioration that has led to consistent cyanobacterial blooms for almost five decades. To describe the possible effects of cyanotoxins on fish (Carrasius gibelio) inhabiting Lake Ludaš, several examinations were performed in November of 2018, including: assessment of water quality (physical and chemical parameters, as well as water microbiology), and assessment of several tissues of C. gibelio (micronucleus test, comet assay, and histopathological analyses). The water quality of Lake Ludaš has been categorized as class III, indicating moderate pollution. Physical and chemical parameters from November of 2018 seemed to be within acceptable levels. Examined fish tissues showed signs of DNA damage (highest in the blood, followed by the liver and gills), and histological alterations (in the liver
and gills). Although other xenobiotics can induce similar changes, cyanotoxins could be the most likely cause of the observed alterations in C. gibelio tissues. The present study demonstrates the need to monitor this important wetland of international significance, since the living organisms in Lake Ludaš are showing signs of environmental stress suggesting that the preservation and natural balance of this ecosystem could be in danger.
PB  - Novi Sad : Faculty of Sciences, Department of Biology and Ecology
T2  - Biologia Serbica
T1  - Harmful effects of cyanobacterial blooms in Lake Ludaš (Serbia) on Carassius gibelio tissues
IS  - 1
VL  - 45
DO  - 10.5281/zenodo.8304662
SP  - 3
EP  - 9
ER  - 

@article{
author = "Takodi, Nada and Drobac Backović, Damjana and Marinović, Zoran and Sunjog, Karolina and Jovanović Marić, Jovana and Kolarević, Stoimir and Kitanović, Nevena and Vuković-Gačić, Branka and Lujić, Jelena and Svirčev, Zorica",
year = "2023",
abstract = "Lake Ludaš is a protected nature reserve in Serbia that has been the focus of scientific interest, due to
perpetual water quality deterioration that has led to consistent cyanobacterial blooms for almost five decades. To describe the possible effects of cyanotoxins on fish (Carrasius gibelio) inhabiting Lake Ludaš, several examinations were performed in November of 2018, including: assessment of water quality (physical and chemical parameters, as well as water microbiology), and assessment of several tissues of C. gibelio (micronucleus test, comet assay, and histopathological analyses). The water quality of Lake Ludaš has been categorized as class III, indicating moderate pollution. Physical and chemical parameters from November of 2018 seemed to be within acceptable levels. Examined fish tissues showed signs of DNA damage (highest in the blood, followed by the liver and gills), and histological alterations (in the liver
and gills). Although other xenobiotics can induce similar changes, cyanotoxins could be the most likely cause of the observed alterations in C. gibelio tissues. The present study demonstrates the need to monitor this important wetland of international significance, since the living organisms in Lake Ludaš are showing signs of environmental stress suggesting that the preservation and natural balance of this ecosystem could be in danger.",
publisher = "Novi Sad : Faculty of Sciences, Department of Biology and Ecology",
journal = "Biologia Serbica",
title = "Harmful effects of cyanobacterial blooms in Lake Ludaš (Serbia) on Carassius gibelio tissues",
number = "1",
volume = "45",
doi = "10.5281/zenodo.8304662",
pages = "3-9"
}

Takodi, N., Drobac Backović, D., Marinović, Z., Sunjog, K., Jovanović Marić, J., Kolarević, S., Kitanović, N., Vuković-Gačić, B., Lujić, J.,& Svirčev, Z.. (2023). Harmful effects of cyanobacterial blooms in Lake Ludaš (Serbia) on Carassius gibelio tissues. in Biologia Serbica
Novi Sad : Faculty of Sciences, Department of Biology and Ecology., 45(1), 3-9.
https://doi.org/10.5281/zenodo.8304662

Takodi N, Drobac Backović D, Marinović Z, Sunjog K, Jovanović Marić J, Kolarević S, Kitanović N, Vuković-Gačić B, Lujić J, Svirčev Z. Harmful effects of cyanobacterial blooms in Lake Ludaš (Serbia) on Carassius gibelio tissues. in Biologia Serbica. 2023;45(1):3-9.
doi:10.5281/zenodo.8304662 .

Takodi, Nada, Drobac Backović, Damjana, Marinović, Zoran, Sunjog, Karolina, Jovanović Marić, Jovana, Kolarević, Stoimir, Kitanović, Nevena, Vuković-Gačić, Branka, Lujić, Jelena, Svirčev, Zorica, "Harmful effects of cyanobacterial blooms in Lake Ludaš (Serbia) on Carassius gibelio tissues" in Biologia Serbica, 45, no. 1 (2023):3-9,
https://doi.org/10.5281/zenodo.8304662 . .

TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - https://academic.oup.com/mutage/advance-article/doi/10.1093/mutage/geac024/6823671
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5243
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford: Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
IS  - 1
VL  - 38
DO  - 10.1093/mutage/geac024
SP  - 21
EP  - 32
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford: Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024",
pages = "21-32"
}

Jovanović Marić, J., Kolarević, S., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford: Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Jelena
AU  - Vuković-Gačić, Branka
AU  - Joksimović, Danijela
AU  - Martinović, Rajko
AU  - Bajt, Oliver
AU  - Ramšak, Andreja
PY  - 2023
UR  - https://academic.oup.com/mutage/advance-article/doi/10.1093/mutage/geac017/6694788
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5121
AB  - In this study, the possible 'vector effect' within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assessment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene.
PB  - Oxford: Oxford University Press
T2  - Mutagenesis
T1  - Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis).
IS  - 1
VL  - 38
DO  - 10.1093/mutage/geac017
SP  - 3
EP  - 12
ER  - 

@article{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Jelena and Vuković-Gačić, Branka and Joksimović, Danijela and Martinović, Rajko and Bajt, Oliver and Ramšak, Andreja",
year = "2023",
abstract = "In this study, the possible 'vector effect' within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assessment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene.",
publisher = "Oxford: Oxford University Press",
journal = "Mutagenesis",
title = "Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis).",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac017",
pages = "3-12"
}

Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, J., Vuković-Gačić, B., Joksimović, D., Martinović, R., Bajt, O.,& Ramšak, A.. (2023). Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis).. in Mutagenesis
Oxford: Oxford University Press., 38(1), 3-12.
https://doi.org/10.1093/mutage/geac017

Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Đorđević J, Vuković-Gačić B, Joksimović D, Martinović R, Bajt O, Ramšak A. Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis).. in Mutagenesis. 2023;38(1):3-12.
doi:10.1093/mutage/geac017 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Jelena, Vuković-Gačić, Branka, Joksimović, Danijela, Martinović, Rajko, Bajt, Oliver, Ramšak, Andreja, "Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)." in Mutagenesis, 38, no. 1 (2023):3-12,
https://doi.org/10.1093/mutage/geac017 . .

TY  - CONF
AU  - Đorđević, Jelena
AU  - Oalđe Pavlović, Mariana
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Duletić-Laušević, Sonja
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5729
AB  - BACKGROUND
According to World Health Organization, 70-95% of the population chooses folk medicine as their primary
approach for health maintenance. In addition to their healing properties and application in folk medicine,
Lamiaceae plants are often valued in cookery as spices and food preservatives.
OBJECTIVES
Hence, the aim of this study was to examine the antibiofilm activity of 12 ethanolic extracts of Lamiaceae species
on biofilm formation and the degradation of existing biofilm of Pseudomonas aeruginosa PAO1 (ATCC 15692).
METHODS
The crystal violet staining method was used to evaluate the newly formed bacterial biofilms. Four concentrations
(double dilutions) of plant extracts (starting conc. 2500 μg/mL), solvent control (ethanol, starting conc.15%),
and positive control (streptomycin, starting conc. 12.5 μg/mL) were tested.082
RESULTS
All ethanol extracts showed antibiofilm activity. However, the strongest activity was observed for Hyssopus
officinalis, Melissa officinalis, Mentha piperita, and Ocimum basilicum, where the viability of bacteria in the
biofilm after treatment was about 60% mainly at each tested concentration. Teucrium chamaedrys extract
exhibited the strongest activity by degrading about 60% of biofilm (about 40% viability) at all concentrations
except at the lowest tested one. Promising results were also observed for M. piperita (at each of the tested
concentrations) and O. basilicum (at the highest tested concentration), where the viability of bacteria in the
biofilm after treatment was reduced by about 40%. The remaining extracts showed a slightly lower effect on
the degradation of the previously formed biofilm of P. aeruginosa PAO1.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?
SP  - 801
EP  - 802
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5729
ER  - 

@conference{
author = "Đorđević, Jelena and Oalđe Pavlović, Mariana and Jovanović Marić, Jovana and Kolarević, Stoimir and Duletić-Laušević, Sonja and Vuković-Gačić, Branka",
year = "2022",
abstract = "BACKGROUND
According to World Health Organization, 70-95% of the population chooses folk medicine as their primary
approach for health maintenance. In addition to their healing properties and application in folk medicine,
Lamiaceae plants are often valued in cookery as spices and food preservatives.
OBJECTIVES
Hence, the aim of this study was to examine the antibiofilm activity of 12 ethanolic extracts of Lamiaceae species
on biofilm formation and the degradation of existing biofilm of Pseudomonas aeruginosa PAO1 (ATCC 15692).
METHODS
The crystal violet staining method was used to evaluate the newly formed bacterial biofilms. Four concentrations
(double dilutions) of plant extracts (starting conc. 2500 μg/mL), solvent control (ethanol, starting conc.15%),
and positive control (streptomycin, starting conc. 12.5 μg/mL) were tested.082
RESULTS
All ethanol extracts showed antibiofilm activity. However, the strongest activity was observed for Hyssopus
officinalis, Melissa officinalis, Mentha piperita, and Ocimum basilicum, where the viability of bacteria in the
biofilm after treatment was about 60% mainly at each tested concentration. Teucrium chamaedrys extract
exhibited the strongest activity by degrading about 60% of biofilm (about 40% viability) at all concentrations
except at the lowest tested one. Promising results were also observed for M. piperita (at each of the tested
concentrations) and O. basilicum (at the highest tested concentration), where the viability of bacteria in the
biofilm after treatment was reduced by about 40%. The remaining extracts showed a slightly lower effect on
the degradation of the previously formed biofilm of P. aeruginosa PAO1.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?",
pages = "801-802",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5729"
}

Đorđević, J., Oalđe Pavlović, M., Jovanović Marić, J., Kolarević, S., Duletić-Laušević, S.,& Vuković-Gačić, B.. (2022). Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 801-802.
https://hdl.handle.net/21.15107/rcub_ibiss_5729

Đorđević J, Oalđe Pavlović M, Jovanović Marić J, Kolarević S, Duletić-Laušević S, Vuković-Gačić B. Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:801-802.
https://hdl.handle.net/21.15107/rcub_ibiss_5729 .

Đorđević, Jelena, Oalđe Pavlović, Mariana, Jovanović Marić, Jovana, Kolarević, Stoimir, Duletić-Laušević, Sonja, Vuković-Gačić, Branka, "Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):801-802,
https://hdl.handle.net/21.15107/rcub_ibiss_5729 .

TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Oswald, Petar
AU  - Oswaldova, Martina
AU  - Slobodnik, Jaroslav
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kološa, Katja
AU  - Žegura, Bojana
AU  - Kolarević, Stoimir
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5725
AB  - Within the Joint Danube Survey 4 (JDS4) (2019) surface water samples
were collected at 24 localities along the Danube River and were extracted by
horizon large volume solid-phase extraction (LVSPE) technique. LVSPE
technique provided 25,000x relative enrichment factor (REF) of the river
samples. The bioactivity of the LVSPE samples was investigated employing
prokaryotic SOS/umuC assay (S9+ and S9-) and battery of bioassays
analyzing different endpoints – cytotoxicity (MTS assay), genotoxicity
(comet assay), cell cycle analyses of ZFL cells and embryo toxicity (zFET) in
eukaryotic models (zebrafish liver cell line – ZFL and Danio rerio embryos –
zebrafish). In the case of the SOS/umuC assay none of the tested samples
(REF100) has induced genotoxic effect. The cytotoxic effect was detected for
11 samples at REF100 (MTS assay). The comet assay results indicated
genotoxic potential for nine samples – one in the Upper Danube, six in the
Middle Danube and two in the Lower Danube. The most potent were
JDS4-37 and JDS4-41 samples collected at two sites in the Serbian part of
the Danube River. These samples were analyzed at REF13. The analysis of
the cell cycle was performed in the case of eight samples for which were
previously recorded genotoxic effect. For one sample (JDS4-41) was found
the G1 phase cell cycle arrest. The zFET assay was performed for all the
samples at REF100 and there was no embryo toxicity or teratogenicity
recorded. The results obtained within this study indicate a higher sensitivity
of eukaryotic models compared to prokaryotic SOS/umuC assay. Likewise, it
was found that most of the genotoxic samples originate from the middle
part of the Danube.
PB  - European Environmental Mutagenesis & Genomics Society
C3  - Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
T1  - Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5725
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Oswald, Petar and Oswaldova, Martina and Slobodnik, Jaroslav and Vuković-Gačić, Branka and Paunović, Momir and Kološa, Katja and Žegura, Bojana and Kolarević, Stoimir",
year = "2022",
abstract = "Within the Joint Danube Survey 4 (JDS4) (2019) surface water samples
were collected at 24 localities along the Danube River and were extracted by
horizon large volume solid-phase extraction (LVSPE) technique. LVSPE
technique provided 25,000x relative enrichment factor (REF) of the river
samples. The bioactivity of the LVSPE samples was investigated employing
prokaryotic SOS/umuC assay (S9+ and S9-) and battery of bioassays
analyzing different endpoints – cytotoxicity (MTS assay), genotoxicity
(comet assay), cell cycle analyses of ZFL cells and embryo toxicity (zFET) in
eukaryotic models (zebrafish liver cell line – ZFL and Danio rerio embryos –
zebrafish). In the case of the SOS/umuC assay none of the tested samples
(REF100) has induced genotoxic effect. The cytotoxic effect was detected for
11 samples at REF100 (MTS assay). The comet assay results indicated
genotoxic potential for nine samples – one in the Upper Danube, six in the
Middle Danube and two in the Lower Danube. The most potent were
JDS4-37 and JDS4-41 samples collected at two sites in the Serbian part of
the Danube River. These samples were analyzed at REF13. The analysis of
the cell cycle was performed in the case of eight samples for which were
previously recorded genotoxic effect. For one sample (JDS4-41) was found
the G1 phase cell cycle arrest. The zFET assay was performed for all the
samples at REF100 and there was no embryo toxicity or teratogenicity
recorded. The results obtained within this study indicate a higher sensitivity
of eukaryotic models compared to prokaryotic SOS/umuC assay. Likewise, it
was found that most of the genotoxic samples originate from the middle
part of the Danube.",
publisher = "European Environmental Mutagenesis & Genomics Society",
journal = "Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands",
title = "Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5725"
}

Kračun-Kolarević, M., Jovanović Marić, J., Oswald, P., Oswaldova, M., Slobodnik, J., Vuković-Gačić, B., Paunović, M., Kološa, K., Žegura, B.,& Kolarević, S.. (2022). Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
European Environmental Mutagenesis & Genomics Society., 75.
https://hdl.handle.net/21.15107/rcub_ibiss_5725

Kračun-Kolarević M, Jovanović Marić J, Oswald P, Oswaldova M, Slobodnik J, Vuković-Gačić B, Paunović M, Kološa K, Žegura B, Kolarević S. Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands. 2022;:75.
https://hdl.handle.net/21.15107/rcub_ibiss_5725 .

Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Oswald, Petar, Oswaldova, Martina, Slobodnik, Jaroslav, Vuković-Gačić, Branka, Paunović, Momir, Kološa, Katja, Žegura, Bojana, Kolarević, Stoimir, "Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique." in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands (2022):75,
https://hdl.handle.net/21.15107/rcub_ibiss_5725 .

TY  - CONF
AU  - Oalđe Pavlović, Mariana
AU  - Đorđević, Jelena
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Duletić-Laušević, Sonja
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5728
AB  - BACKGROUND
The increasing bacterial resistance to antibiotics poses a serious global threat. For that reason, the research into plant-derived antibacterial agents has become progressively more interesting, particularly in regard to their capacity to prevent the delay or inability of wound closure and healing.
OBJECTIVES
The aim of this study was to evaluate i) the antibacterial potential of ethanolic extracts (70% ethanol) of 18
Lamiaceae species against four Gram-negative and three Gram-positive bacterial strains; ii) the effect of these extracts on Pseudomonas aeruginosa PAO1 infection of human lung fibroblasts (MRC-5), and iii) the effect on MRC-5 cell migration.
METHODS
The antibacterial activity was tested by minimal inhibitory concentration (MIC) assay. The effect on bacterial infection of MRC-5 cells was determined using the invasion assay, while the cell migration was assessed with Scratch assay.
RESULTS
The tested Gram-positive bacteria, especially Bacillus subtilis, are more sensitive to the effects of ethanolic extracts than the Gram-negative bacteria. Moreover, the tested extracts significantly inhibited the invasion of Pseudomonas aeruginosa PAO1 during MRC-5 infection, and most of them also displayed promising stimulating potential on MRC-5 migration in a disrupted cell monolayer. Finally, the results of IBR (integrated biomarker response) analysis highlighted Salvia officinalis ethanolic extract as the most active one, which is in accordance with the existing data suggesting that this plant represents an effective antibacterial and wound-healing agent commonly used in traditional medicine. Altogether, these results are expected to foster further studies that will focus on combating bacterial resistance to antibiotics using natural products.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Ethanolic extracts of Lamiaceae species inhibit the bacterial infection of human lung fibroblasts and stimulate cell migration
SP  - 799
EP  - 800
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5728
ER  - 

@conference{
author = "Oalđe Pavlović, Mariana and Đorđević, Jelena and Jovanović Marić, Jovana and Kolarević, Stoimir and Duletić-Laušević, Sonja and Vuković-Gačić, Branka",
year = "2022",
abstract = "BACKGROUND
The increasing bacterial resistance to antibiotics poses a serious global threat. For that reason, the research into plant-derived antibacterial agents has become progressively more interesting, particularly in regard to their capacity to prevent the delay or inability of wound closure and healing.
OBJECTIVES
The aim of this study was to evaluate i) the antibacterial potential of ethanolic extracts (70% ethanol) of 18
Lamiaceae species against four Gram-negative and three Gram-positive bacterial strains; ii) the effect of these extracts on Pseudomonas aeruginosa PAO1 infection of human lung fibroblasts (MRC-5), and iii) the effect on MRC-5 cell migration.
METHODS
The antibacterial activity was tested by minimal inhibitory concentration (MIC) assay. The effect on bacterial infection of MRC-5 cells was determined using the invasion assay, while the cell migration was assessed with Scratch assay.
RESULTS
The tested Gram-positive bacteria, especially Bacillus subtilis, are more sensitive to the effects of ethanolic extracts than the Gram-negative bacteria. Moreover, the tested extracts significantly inhibited the invasion of Pseudomonas aeruginosa PAO1 during MRC-5 infection, and most of them also displayed promising stimulating potential on MRC-5 migration in a disrupted cell monolayer. Finally, the results of IBR (integrated biomarker response) analysis highlighted Salvia officinalis ethanolic extract as the most active one, which is in accordance with the existing data suggesting that this plant represents an effective antibacterial and wound-healing agent commonly used in traditional medicine. Altogether, these results are expected to foster further studies that will focus on combating bacterial resistance to antibiotics using natural products.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Ethanolic extracts of Lamiaceae species inhibit the bacterial infection of human lung fibroblasts and stimulate cell migration",
pages = "799-800",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5728"
}

Oalđe Pavlović, M., Đorđević, J., Jovanović Marić, J., Kolarević, S., Duletić-Laušević, S.,& Vuković-Gačić, B.. (2022). Ethanolic extracts of Lamiaceae species inhibit the bacterial infection of human lung fibroblasts and stimulate cell migration. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 799-800.
https://hdl.handle.net/21.15107/rcub_ibiss_5728

Oalđe Pavlović M, Đorđević J, Jovanović Marić J, Kolarević S, Duletić-Laušević S, Vuković-Gačić B. Ethanolic extracts of Lamiaceae species inhibit the bacterial infection of human lung fibroblasts and stimulate cell migration. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:799-800.
https://hdl.handle.net/21.15107/rcub_ibiss_5728 .

Oalđe Pavlović, Mariana, Đorđević, Jelena, Jovanović Marić, Jovana, Kolarević, Stoimir, Duletić-Laušević, Sonja, Vuković-Gačić, Branka, "Ethanolic extracts of Lamiaceae species inhibit the bacterial infection of human lung fibroblasts and stimulate cell migration" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):799-800,
https://hdl.handle.net/21.15107/rcub_ibiss_5728 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5724
AB  - The middle section of the Danube River basin in the previous three Joint
Danube Surveys (JDS) was found to be under intensive pollution, mainly
affected by wastewaters. As a consequence of the lack of wastewater
treatment plants, complex xenobiotics mixtures may get into surface water
and decrease water quality and organisms’ health. Some xenobiotics may
induce different types of DNA damage. Hence, DNA damage could be a
useful biomarker in the detection of “early warning signals” of organisms’
exposure to genotoxic compounds, while the usage of bioassays batteries
can provide better insight into a genotoxic potential. This study was
conducted in 2019, during the JDS4 expedition, to assess the genotoxic
potential of nine sites on the section of the Danube River which goes
through Serbia and its major tributaries: the Sava, Tisza and Velika Morava.
Blood and muscle of Alburnus alburnus (bleak) were used for genotoxicity
assessment by comet, micronucleus and RAPD assays. In comet assay, the
highest level of DNA damage was recorded at the Velika Morava mouth (JDS
39) and on the Danube River, downstream Radujevac (JDS 41). The highest
frequency of micronucleus was recorded at the Sava mouth (JDS 36) and on
the Danube River, downstream Pančevo (JDS 37). The lowest level of DNA
damage in both tests was recorded on the Sava, site Jamena (JDS 35). The
RAPD analysis distinct three major groups: Tisza mouth (JDS 33) and sites
on the Sava (JDS 35, JDS 36), then two sites on the Danube River (JDS 37,
JDS 41) and one site on the Danube River, Ram (JDS 40) with the Velika
Morava mouth (JDS 39). Analyzed bioassays showed different sensitivity
where the comet assay had the highest potential in discrimination of sites
based on genotoxicity. Bleak was proved to be a reliable bioindicator in eco/
genotoxicological studies.
PB  - European Environmental Mutagenesis & Genomics Society
C3  - Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
T1  - Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries.
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5724
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "The middle section of the Danube River basin in the previous three Joint
Danube Surveys (JDS) was found to be under intensive pollution, mainly
affected by wastewaters. As a consequence of the lack of wastewater
treatment plants, complex xenobiotics mixtures may get into surface water
and decrease water quality and organisms’ health. Some xenobiotics may
induce different types of DNA damage. Hence, DNA damage could be a
useful biomarker in the detection of “early warning signals” of organisms’
exposure to genotoxic compounds, while the usage of bioassays batteries
can provide better insight into a genotoxic potential. This study was
conducted in 2019, during the JDS4 expedition, to assess the genotoxic
potential of nine sites on the section of the Danube River which goes
through Serbia and its major tributaries: the Sava, Tisza and Velika Morava.
Blood and muscle of Alburnus alburnus (bleak) were used for genotoxicity
assessment by comet, micronucleus and RAPD assays. In comet assay, the
highest level of DNA damage was recorded at the Velika Morava mouth (JDS
39) and on the Danube River, downstream Radujevac (JDS 41). The highest
frequency of micronucleus was recorded at the Sava mouth (JDS 36) and on
the Danube River, downstream Pančevo (JDS 37). The lowest level of DNA
damage in both tests was recorded on the Sava, site Jamena (JDS 35). The
RAPD analysis distinct three major groups: Tisza mouth (JDS 33) and sites
on the Sava (JDS 35, JDS 36), then two sites on the Danube River (JDS 37,
JDS 41) and one site on the Danube River, Ram (JDS 40) with the Velika
Morava mouth (JDS 39). Analyzed bioassays showed different sensitivity
where the comet assay had the highest potential in discrimination of sites
based on genotoxicity. Bleak was proved to be a reliable bioindicator in eco/
genotoxicological studies.",
publisher = "European Environmental Mutagenesis & Genomics Society",
journal = "Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands",
title = "Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries.",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5724"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
European Environmental Mutagenesis & Genomics Society., 69.
https://hdl.handle.net/21.15107/rcub_ibiss_5724

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands. 2022;:69.
https://hdl.handle.net/21.15107/rcub_ibiss_5724 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries." in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands (2022):69,
https://hdl.handle.net/21.15107/rcub_ibiss_5724 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5722
AB  - Within the past decade, our research group placed great effort in
exploration of the impact of treated/untreated wastewaters on aquatic
ecosystems as less than 13 % of collected municipal wastewaters are
processed before their release to receiving waters in Serbia. Having in mind
that more than 90% of the rivers within the territory of Serbia belong to the
Danube River Basin, our major focus is naturally on the large transnational
waters such as the Danube and Sava rivers. Depended on the concept of the
study and hydro-morphological characteristics of the sites, various
approaches have been employed for in situ assessment of eco/geno-toxicity
which use different organisms in passive and active biomonitoring. Aquatic
organisms of different trophic levels have diverse life strategies, metabolism
pathways, and consequently, they have a different response to pollutant
pressure. In eco/geno-toxicological studies we have successfully employed
several species of freshwater mussels (Unio tumidus, Unio pictorum and
Sinanodonta woodiana) as well as few economically important fish species
(common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak
(Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary
choice for the assessment of DNA damage considering its sensitivity and
cost effectiveness. Selection of certain types of tissues for the assessment
(such as blood or haemolymph), which do not require sacrificing of the
animals or additional manipulation in preparation of cell suspension coupled
with mini-gel format of the slides enables high-throughput screening of the
genotoxic potential in situ. Additionally, cryopreservation of the blood
samples directly onsite expands the research area as it overcomes issues
related to safe transportation of the samples to the laboratory. Data
obtained so far indicated that the genotoxic response in the studied
indicator organisms significantly differ which is understandable considering
the difference in uptake, accumulation and physiological responses.
PB  - European Environmental Mutagenesis & Genomics Society
C3  - Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
T1  - Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research.
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5722
ER  - 

@conference{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2022",
abstract = "Within the past decade, our research group placed great effort in
exploration of the impact of treated/untreated wastewaters on aquatic
ecosystems as less than 13 % of collected municipal wastewaters are
processed before their release to receiving waters in Serbia. Having in mind
that more than 90% of the rivers within the territory of Serbia belong to the
Danube River Basin, our major focus is naturally on the large transnational
waters such as the Danube and Sava rivers. Depended on the concept of the
study and hydro-morphological characteristics of the sites, various
approaches have been employed for in situ assessment of eco/geno-toxicity
which use different organisms in passive and active biomonitoring. Aquatic
organisms of different trophic levels have diverse life strategies, metabolism
pathways, and consequently, they have a different response to pollutant
pressure. In eco/geno-toxicological studies we have successfully employed
several species of freshwater mussels (Unio tumidus, Unio pictorum and
Sinanodonta woodiana) as well as few economically important fish species
(common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak
(Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary
choice for the assessment of DNA damage considering its sensitivity and
cost effectiveness. Selection of certain types of tissues for the assessment
(such as blood or haemolymph), which do not require sacrificing of the
animals or additional manipulation in preparation of cell suspension coupled
with mini-gel format of the slides enables high-throughput screening of the
genotoxic potential in situ. Additionally, cryopreservation of the blood
samples directly onsite expands the research area as it overcomes issues
related to safe transportation of the samples to the laboratory. Data
obtained so far indicated that the genotoxic response in the studied
indicator organisms significantly differ which is understandable considering
the difference in uptake, accumulation and physiological responses.",
publisher = "European Environmental Mutagenesis & Genomics Society",
journal = "Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands",
title = "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research.",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5722"
}

Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Gačić, Z.,& Vuković-Gačić, B.. (2022). Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
European Environmental Mutagenesis & Genomics Society., 42.
https://hdl.handle.net/21.15107/rcub_ibiss_5722

Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Đorđević J, Paunović M, Kostić-Vuković J, Sunjog K, Gačić Z, Vuković-Gačić B. Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands. 2022;:42.
https://hdl.handle.net/21.15107/rcub_ibiss_5722 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Gačić, Zoran, Vuković-Gačić, Branka, "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research." in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands (2022):42,
https://hdl.handle.net/21.15107/rcub_ibiss_5722 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Ana
AU  - Jovanović Marić, Jovana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander K.T.
AU  - Farnleitner, Andreas H.
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6235
AB  - BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters
SP  - 614
EP  - 615
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6235
ER  - 

@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Đorđević, Ana and Jovanović Marić, Jovana and Vojnović-Milutinović, Danijela and Kirschner, Alexander K.T. and Farnleitner, Andreas H. and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2022",
abstract = "BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters",
pages = "614-615",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6235"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Đorđević, A., Jovanović Marić, J., Vojnović-Milutinović, D., Kirschner, A. K.T., Farnleitner, A. H., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2022). Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 614-615.
https://hdl.handle.net/21.15107/rcub_ibiss_6235

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Đorđević A, Jovanović Marić J, Vojnović-Milutinović D, Kirschner AK, Farnleitner AH, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:614-615.
https://hdl.handle.net/21.15107/rcub_ibiss_6235 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Đorđević, Ana, Jovanović Marić, Jovana, Vojnović-Milutinović, Danijela, Kirschner, Alexander K.T., Farnleitner, Andreas H., Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):614-615,
https://hdl.handle.net/21.15107/rcub_ibiss_6235 .

TY  - CONF
AU  - Martinović, Rajko
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Jelena
AU  - Jovanović Marić, Jovana
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
AU  - Petrošević Bajčeta, Ana
AU  - Joksimović, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5726
AB  - More frequent application of biological parameters beside the conventional chemical parameters in national
monitoring for marine ecosystem assessment is supported by UNEP/MAP. Accordingly, to evaluate the
possible effects of pollution by determination of trace element content and genotoxicity assessment in
marine organisms within the Boka Kotorska Bay, Montenegro (southern Adriatic), we carried a field study
which included transplantation of the seabream (Sparus aurata) from aquaculture farm (Orahovac) to more
impacted sites (Dobrota and port of Tivat), situated in vicinity of the main ports. On the sampling site
Orahovac, the group of seabream specimens were placed in closed fish trap on 5 m depth (O1), another fish
trap was placed in Dobrota, while the third fish trap with seabreams was placed at the site port of Tivat.
The additional group was sampled from standard aquaculture cage on sampling site (O2) to reduce possible
bias in genotoxicity data due to confined space within the fish trap. After two weeks of exposure, blood
from fish heart was taken, afterwards muscle tissues were prepared for trace element determination.
Genotoxicity was measured by DNA damage induction based on comet (single cell gel electrophoresis)
assay parameter – Tail intensity (TI%), while trace element (As, Cd, Pb, Hg, Cr, Cu, Fe, Mn, Ni, Zn and
V) content was determined by ICP. Mean values of TI% obtained from seabream specimens at the sampling
site Orahovac were: 4.26 ± 0.17 – O1 and 2.56 ± 0.11 – O2, while TI% values for transplanted specimens
on the sites Dobrota and port of Tivat were 6.38 ± 0.17 and 11.06 ± 0.23, respectively. Statistically
significant differences (p<0,05) of TI% were observed between all specimens groups. The group of
seabream from port of Tivat showed the highest TI% (higher level of DNA damage), most probably caused
by marine pollution. Obtained trace element concentrations in seabream tissues were significantly lower in
comparison to values recommended by FAO, EC and national legislatives of Croatia, Spain and Turkey.
Since transplanted fish showed a significant response in a relatively short exposure period, our results
support the introduction of seabream caging as monitoring tool to reveal marine pollution by multiple
biomarker approach. Considering the significant share of aquacultured seabream in human consumption,
higher availability for sampling and convenience in application for molecular biomarker analyses in the
same samples, such an integrated monitoring approach would contribute to more credible data.
PB  - Split: Institute of Oceanography and Fisheries
C3  - Book of Abstracts: 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia
T1  - Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5726
ER  - 

@conference{
author = "Martinović, Rajko and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević, Jelena and Jovanović Marić, Jovana and Gačić, Zoran and Vuković-Gačić, Branka and Petrošević Bajčeta, Ana and Joksimović, Danijela",
year = "2022",
abstract = "More frequent application of biological parameters beside the conventional chemical parameters in national
monitoring for marine ecosystem assessment is supported by UNEP/MAP. Accordingly, to evaluate the
possible effects of pollution by determination of trace element content and genotoxicity assessment in
marine organisms within the Boka Kotorska Bay, Montenegro (southern Adriatic), we carried a field study
which included transplantation of the seabream (Sparus aurata) from aquaculture farm (Orahovac) to more
impacted sites (Dobrota and port of Tivat), situated in vicinity of the main ports. On the sampling site
Orahovac, the group of seabream specimens were placed in closed fish trap on 5 m depth (O1), another fish
trap was placed in Dobrota, while the third fish trap with seabreams was placed at the site port of Tivat.
The additional group was sampled from standard aquaculture cage on sampling site (O2) to reduce possible
bias in genotoxicity data due to confined space within the fish trap. After two weeks of exposure, blood
from fish heart was taken, afterwards muscle tissues were prepared for trace element determination.
Genotoxicity was measured by DNA damage induction based on comet (single cell gel electrophoresis)
assay parameter – Tail intensity (TI%), while trace element (As, Cd, Pb, Hg, Cr, Cu, Fe, Mn, Ni, Zn and
V) content was determined by ICP. Mean values of TI% obtained from seabream specimens at the sampling
site Orahovac were: 4.26 ± 0.17 – O1 and 2.56 ± 0.11 – O2, while TI% values for transplanted specimens
on the sites Dobrota and port of Tivat were 6.38 ± 0.17 and 11.06 ± 0.23, respectively. Statistically
significant differences (p<0,05) of TI% were observed between all specimens groups. The group of
seabream from port of Tivat showed the highest TI% (higher level of DNA damage), most probably caused
by marine pollution. Obtained trace element concentrations in seabream tissues were significantly lower in
comparison to values recommended by FAO, EC and national legislatives of Croatia, Spain and Turkey.
Since transplanted fish showed a significant response in a relatively short exposure period, our results
support the introduction of seabream caging as monitoring tool to reveal marine pollution by multiple
biomarker approach. Considering the significant share of aquacultured seabream in human consumption,
higher availability for sampling and convenience in application for molecular biomarker analyses in the
same samples, such an integrated monitoring approach would contribute to more credible data.",
publisher = "Split: Institute of Oceanography and Fisheries",
journal = "Book of Abstracts: 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia",
title = "Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5726"
}

Martinović, R., Kolarević, S., Kračun-Kolarević, M., Đorđević, J., Jovanović Marić, J., Gačić, Z., Vuković-Gačić, B., Petrošević Bajčeta, A.,& Joksimović, D.. (2022). Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast. in Book of Abstracts: 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia
Split: Institute of Oceanography and Fisheries., 33.
https://hdl.handle.net/21.15107/rcub_ibiss_5726

Martinović R, Kolarević S, Kračun-Kolarević M, Đorđević J, Jovanović Marić J, Gačić Z, Vuković-Gačić B, Petrošević Bajčeta A, Joksimović D. Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast. in Book of Abstracts: 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. 2022;:33.
https://hdl.handle.net/21.15107/rcub_ibiss_5726 .

Martinović, Rajko, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević, Jelena, Jovanović Marić, Jovana, Gačić, Zoran, Vuković-Gačić, Branka, Petrošević Bajčeta, Ana, Joksimović, Danijela, "Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast" in Book of Abstracts: 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia (2022):33,
https://hdl.handle.net/21.15107/rcub_ibiss_5726 .

TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Nikolić, Stefan
AU  - Jovanović Marić, Jovana
AU  - Ilić, Marija
AU  - Đukić, Aleksandar
AU  - Paunović, Momir
AU  - Kolarević, Stoimir
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6589
AB  - U ovom radu ispitivan je stepen kontaminacije vode i plavne zone reke Save na primeru ispusta otpadnih voda kod Beogradskog sajma korišćenjem mikrobioloških indikatora fekalnog zagađenja. Kvantifikacija ukupnih koliforma, fekalnih koliforma i fekalnih enterokoka rađena je pomoću Colilert-18 i Enterolert-E sistema. Analiza broja spora klostridija rađena je membranskom filtracijom u skladu sa standardom ISO 14189:2013. Rezultati mikrobioloških analiza ukazuju na visok stepen kontaminacije vode i plavnog područja na ispitivanom lokalitetu, kao i na potencijalni rizik koje zabeleženo fekalno zagađenje može predstavljati po zdravlje osoba koje ispitivano područje koriste za rekreaciju.
AB  - In this paper, the level of contamination of the Sava River water and floodplain was asssessed using microbiological indicators of fecal pollution. Wastewater outlet near the Belgrade Fair was chosen as a case study. Quantification of total coliforms, faecal coliforms and fecal enterococci was performed using the Colilert-18 and Enterolert-E systems. The analysis of the number of clostridian spores was carried out by membrane filtration in accordance with the ISO 14189 standard. The results of microbiological analyzes indicate a high degree of contamination of the water and floodplain area at the investigated location, as well as the potential risk that detected fecal pollution may represent for the health of persons who use the investigated area for recreation.
PB  - Belgarde: Serbian Association for Water Protection
C3  - Conferenece proceedings: 51st Annual Conference of the Serbian Water Pollution Control Society: Water 2022; 2022 Oct 26-28; Vrnjačka Banja, Serbia
T1  - Procena kontaminacije vode i plavne zone reke Save na mestu ispusta otpadnih voda korišćenjem mikrobioloških indikatora fekalnog zagađenja
T1  - Contamination of the Sava River water and floodplain at the wastewater outlet site assessed by microbiological indicators of faecal pollution
DO  - 77743881
SP  - 191
EP  - 196
ER  - 

@conference{
editor = "Đukić, Aleksandar",
author = "Kračun-Kolarević, Margareta and Nikolić, Stefan and Jovanović Marić, Jovana and Ilić, Marija and Đukić, Aleksandar and Paunović, Momir and Kolarević, Stoimir",
year = "2022",
abstract = "U ovom radu ispitivan je stepen kontaminacije vode i plavne zone reke Save na primeru ispusta otpadnih voda kod Beogradskog sajma korišćenjem mikrobioloških indikatora fekalnog zagađenja. Kvantifikacija ukupnih koliforma, fekalnih koliforma i fekalnih enterokoka rađena je pomoću Colilert-18 i Enterolert-E sistema. Analiza broja spora klostridija rađena je membranskom filtracijom u skladu sa standardom ISO 14189:2013. Rezultati mikrobioloških analiza ukazuju na visok stepen kontaminacije vode i plavnog područja na ispitivanom lokalitetu, kao i na potencijalni rizik koje zabeleženo fekalno zagađenje može predstavljati po zdravlje osoba koje ispitivano područje koriste za rekreaciju., In this paper, the level of contamination of the Sava River water and floodplain was asssessed using microbiological indicators of fecal pollution. Wastewater outlet near the Belgrade Fair was chosen as a case study. Quantification of total coliforms, faecal coliforms and fecal enterococci was performed using the Colilert-18 and Enterolert-E systems. The analysis of the number of clostridian spores was carried out by membrane filtration in accordance with the ISO 14189 standard. The results of microbiological analyzes indicate a high degree of contamination of the water and floodplain area at the investigated location, as well as the potential risk that detected fecal pollution may represent for the health of persons who use the investigated area for recreation.",
publisher = "Belgarde: Serbian Association for Water Protection",
journal = "Conferenece proceedings: 51st Annual Conference of the Serbian Water Pollution Control Society: Water 2022; 2022 Oct 26-28; Vrnjačka Banja, Serbia",
title = "Procena kontaminacije vode i plavne zone reke Save na mestu ispusta otpadnih voda korišćenjem mikrobioloških indikatora fekalnog zagađenja, Contamination of the Sava River water and floodplain at the wastewater outlet site assessed by microbiological indicators of faecal pollution",
doi = "77743881",
pages = "191-196"
}

Đukić, A., Kračun-Kolarević, M., Nikolić, S., Jovanović Marić, J., Ilić, M., Đukić, A., Paunović, M.,& Kolarević, S.. (2022). Procena kontaminacije vode i plavne zone reke Save na mestu ispusta otpadnih voda korišćenjem mikrobioloških indikatora fekalnog zagađenja. in Conferenece proceedings: 51st Annual Conference of the Serbian Water Pollution Control Society: Water 2022; 2022 Oct 26-28; Vrnjačka Banja, Serbia
Belgarde: Serbian Association for Water Protection., 191-196.
https://doi.org/77743881

Đukić A, Kračun-Kolarević M, Nikolić S, Jovanović Marić J, Ilić M, Đukić A, Paunović M, Kolarević S. Procena kontaminacije vode i plavne zone reke Save na mestu ispusta otpadnih voda korišćenjem mikrobioloških indikatora fekalnog zagađenja. in Conferenece proceedings: 51st Annual Conference of the Serbian Water Pollution Control Society: Water 2022; 2022 Oct 26-28; Vrnjačka Banja, Serbia. 2022;:191-196.
doi:77743881 .

Đukić, Aleksandar, Kračun-Kolarević, Margareta, Nikolić, Stefan, Jovanović Marić, Jovana, Ilić, Marija, Đukić, Aleksandar, Paunović, Momir, Kolarević, Stoimir, "Procena kontaminacije vode i plavne zone reke Save na mestu ispusta otpadnih voda korišćenjem mikrobioloških indikatora fekalnog zagađenja" in Conferenece proceedings: 51st Annual Conference of the Serbian Water Pollution Control Society: Water 2022; 2022 Oct 26-28; Vrnjačka Banja, Serbia (2022):191-196,
https://doi.org/77743881 . .

TY  - CONF
AU  - Đorđević, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Oalđe Pavlović, Mariana
AU  - Sladić, Dušan
AU  - Novaković, Irena
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6588
AB  - Rak je vodeći uzrok smrti ljudi širom sveta sa 10 miliona umrlih u 2020. godini
zbog čega je jedan od imperativa naučne zajednice pronalazak efikasnijih
hemioterapeutika. Više od polovine lekova prirodnog je porekla, a budući da
okeani čine oko 70% Zemljine površine, marinski ekosistem je odlična osnova za
dobijanje novih lekova. Hinoni avarol i avaron su redoks par izolovan iz
mediteranskog sunđera Disidea avara i pokazuju raznovrsnu biološku aktivnost
poput antimikrobne i antitumorske. Кao i kod većine marinskih organizama,
najveće probleme za njihovu primenu predstavljaju slab prinos i cena
eksploatacije. Rešenje problema može biti sinteza jedinjenja sličnih po
hemijskoj strukturi i delovanju avarolu/avaronu poput terc-butilhinona (TBQ),
a sintezom alkiltio i ariltio derivata dodatno povećanje njegove aktivnosti.
Uporedno je testiran antibakterijski, toksični, citotoksični, genotoksični,
antioksidativni i antineurodegenerativni potencijal TBQ i njegovih derivata.
Generalno, derivati TBQ pokazuju jaču biološku aktivnost. Najjača
antibakterijska aktivnost uočena je na S. aureus i B. subtilis. Modifikacije
povećavaju citotoksičnost i genotoksičnost na humanim ćelijskim linijama dok
derivat 2-terc-butil-5,6-(etileneditio)-1,4-benzohinon pokazuje najjaču
aktivnost i na osnovu γH2AX testa dovodi do dvolančanih prekida DNК
molekula. Sva ispitivana jedinjenja snažno zaustavljaju ćelijski ciklus u G0/G1
fazi. Dodatno, jedinjenja pokazuju dobar antioksidativni i anttineurodegenerativni potencijal.
AB  - Рак је водећи узрок смрти људи широм света са 10 милиона умрлих у 2020. години
због чега је један од императива научне заједнице проналазак ефикаснијих
хемиотерапеутика. Више од половине лекова природног је порекла, а будући да
океани чине око 70% Земљине површине, марински екосистем је одлична основа за
добијање нових лекова. Хинони аварол и аварон су редокс пар изолован из
медитеранског сунђера Disidea avara и показују разноврсну биолошку активност
попут антимикробне и антитуморске. Као и код већине маринских организама,
највећe проблемe за њихову примену представљају слаб принос и цена
експлоатације. Решење проблема може бити синтеза једињења сличних по
хемијској структури и деловању аваролу/аварону попут терц-бутилхинона (ТBQ),
а синтезом алкилтио и арилтио деривата додатно повећање његове активности.
Упоредно је тестиран антибактеријски, токсични, цитотоксични, генотоксични,
антиоксидативни и антинеуродегенеративни потенцијал ТBQ и његових деривата.
Генерално, деривати ТBQ показују јачу биолошку активност. Најјача
антибактеријска активност уочена је на S. aureus и B. subtilis. Модификације
повећавају цитотоксичност и генотоксичност на хуманим ћелијским линијама док
дериват 2-терц-бутил-5,6-(етиленедитио)-1,4-бензохинон показује најјачу
активност и на основу γH2AX теста доводи до дволанчаних прекида ДНК
молекула. Сва испитивана једињења снажно заустављају ћелијски циклус у Г0/Г1
фази. Додатно, једињења показују добар антиоксидативни и
анттинеуродегенеративни потенцијал.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata.
T1  - Упоредни приказ биолошкe активности потенцијалних антитуморских агенаса: терц-бутилхинона и његових алкилтио и арилтио деривата
SP  - 251
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6588
ER  - 

@conference{
author = "Đorđević, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Oalđe Pavlović, Mariana and Sladić, Dušan and Novaković, Irena and Vuković-Gačić, Branka",
year = "2022",
abstract = "Rak je vodeći uzrok smrti ljudi širom sveta sa 10 miliona umrlih u 2020. godini
zbog čega je jedan od imperativa naučne zajednice pronalazak efikasnijih
hemioterapeutika. Više od polovine lekova prirodnog je porekla, a budući da
okeani čine oko 70% Zemljine površine, marinski ekosistem je odlična osnova za
dobijanje novih lekova. Hinoni avarol i avaron su redoks par izolovan iz
mediteranskog sunđera Disidea avara i pokazuju raznovrsnu biološku aktivnost
poput antimikrobne i antitumorske. Кao i kod većine marinskih organizama,
najveće probleme za njihovu primenu predstavljaju slab prinos i cena
eksploatacije. Rešenje problema može biti sinteza jedinjenja sličnih po
hemijskoj strukturi i delovanju avarolu/avaronu poput terc-butilhinona (TBQ),
a sintezom alkiltio i ariltio derivata dodatno povećanje njegove aktivnosti.
Uporedno je testiran antibakterijski, toksični, citotoksični, genotoksični,
antioksidativni i antineurodegenerativni potencijal TBQ i njegovih derivata.
Generalno, derivati TBQ pokazuju jaču biološku aktivnost. Najjača
antibakterijska aktivnost uočena je na S. aureus i B. subtilis. Modifikacije
povećavaju citotoksičnost i genotoksičnost na humanim ćelijskim linijama dok
derivat 2-terc-butil-5,6-(etileneditio)-1,4-benzohinon pokazuje najjaču
aktivnost i na osnovu γH2AX testa dovodi do dvolančanih prekida DNК
molekula. Sva ispitivana jedinjenja snažno zaustavljaju ćelijski ciklus u G0/G1
fazi. Dodatno, jedinjenja pokazuju dobar antioksidativni i anttineurodegenerativni potencijal., Рак је водећи узрок смрти људи широм света са 10 милиона умрлих у 2020. години
због чега је један од императива научне заједнице проналазак ефикаснијих
хемиотерапеутика. Више од половине лекова природног је порекла, а будући да
океани чине око 70% Земљине површине, марински екосистем је одлична основа за
добијање нових лекова. Хинони аварол и аварон су редокс пар изолован из
медитеранског сунђера Disidea avara и показују разноврсну биолошку активност
попут антимикробне и антитуморске. Као и код већине маринских организама,
највећe проблемe за њихову примену представљају слаб принос и цена
експлоатације. Решење проблема може бити синтеза једињења сличних по
хемијској структури и деловању аваролу/аварону попут терц-бутилхинона (ТBQ),
а синтезом алкилтио и арилтио деривата додатно повећање његове активности.
Упоредно је тестиран антибактеријски, токсични, цитотоксични, генотоксични,
антиоксидативни и антинеуродегенеративни потенцијал ТBQ и његових деривата.
Генерално, деривати ТBQ показују јачу биолошку активност. Најјача
антибактеријска активност уочена је на S. aureus и B. subtilis. Модификације
повећавају цитотоксичност и генотоксичност на хуманим ћелијским линијама док
дериват 2-терц-бутил-5,6-(етиленедитио)-1,4-бензохинон показује најјачу
активност и на основу γH2AX теста доводи до дволанчаних прекида ДНК
молекула. Сва испитивана једињења снажно заустављају ћелијски циклус у Г0/Г1
фази. Додатно, једињења показују добар антиоксидативни и
анттинеуродегенеративни потенцијал.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata., Упоредни приказ биолошкe активности потенцијалних антитуморских агенаса: терц-бутилхинона и његових алкилтио и арилтио деривата",
pages = "251",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6588"
}

Đorđević, J., Kolarević, S., Jovanović Marić, J., Oalđe Pavlović, M., Sladić, D., Novaković, I.,& Vuković-Gačić, B.. (2022). Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata.. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 251.
https://hdl.handle.net/21.15107/rcub_ibiss_6588

Đorđević J, Kolarević S, Jovanović Marić J, Oalđe Pavlović M, Sladić D, Novaković I, Vuković-Gačić B. Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata.. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:251.
https://hdl.handle.net/21.15107/rcub_ibiss_6588 .

Đorđević, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Oalđe Pavlović, Mariana, Sladić, Dušan, Novaković, Irena, Vuković-Gačić, Branka, "Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata." in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):251,
https://hdl.handle.net/21.15107/rcub_ibiss_6588 .

TY  - JOUR
AU  - Đorđević, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović-Marić, Jovana
AU  - Oalđe-Pavlović, Mariana
AU  - Sladić, Dušan
AU  - Novaković, Irena
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0352-51392200044D
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5171
AB  - Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.
PB  - Belgrade: Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone
DO  - 10.2298/JSC220304044D
ER  - 

@article{
author = "Đorđević, Jelena and Kolarević, Stoimir and Jovanović-Marić, Jovana and Oalđe-Pavlović, Mariana and Sladić, Dušan and Novaković, Irena and Vuković-Gačić, Branka",
year = "2022",
abstract = "Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone",
doi = "10.2298/JSC220304044D"
}

Đorđević, J., Kolarević, S., Jovanović-Marić, J., Oalđe-Pavlović, M., Sladić, D., Novaković, I.,& Vuković-Gačić, B.. (2022). Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Society..
https://doi.org/10.2298/JSC220304044D

Đorđević J, Kolarević S, Jovanović-Marić J, Oalđe-Pavlović M, Sladić D, Novaković I, Vuković-Gačić B. Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society. 2022;.
doi:10.2298/JSC220304044D .

Đorđević, Jelena, Kolarević, Stoimir, Jovanović-Marić, Jovana, Oalđe-Pavlović, Mariana, Sladić, Dušan, Novaković, Irena, Vuković-Gačić, Branka, "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone" in Journal of the Serbian Chemical Society (2022),
https://doi.org/10.2298/JSC220304044D . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Jovanović Marić, Jovana
AU  - Kirschner, Alexander K T
AU  - Farnleitner, Andreas A H
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S004896972204061X
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9232394
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5072
AB  - Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.
PB  - Amsterdam: Elsevier
T2  - Science of The Total Environment
T1  - Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.
VL  - 843
DO  - 10.1016/j.scitotenv.2022.156964
SP  - 156964
ER  - 

@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Đorđević, Ana and Vojnović-Milutinović, Danijela and Jovanović Marić, Jovana and Kirschner, Alexander K T and Farnleitner, Andreas A H and Linke, Rita and Đukić, Aleksandar and Kostić-Vuković, Jovana and Paunović, Momir",
year = "2022",
abstract = "Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.",
publisher = "Amsterdam: Elsevier",
journal = "Science of The Total Environment",
title = "Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.",
volume = "843",
doi = "10.1016/j.scitotenv.2022.156964",
pages = "156964"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Đorđević, A., Vojnović-Milutinović, D., Jovanović Marić, J., Kirschner, A. K. T., Farnleitner, A. A. H., Linke, R., Đukić, A., Kostić-Vuković, J.,& Paunović, M.. (2022). Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.. in Science of The Total Environment
Amsterdam: Elsevier., 843, 156964.
https://doi.org/10.1016/j.scitotenv.2022.156964

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Đorđević A, Vojnović-Milutinović D, Jovanović Marić J, Kirschner AKT, Farnleitner AAH, Linke R, Đukić A, Kostić-Vuković J, Paunović M. Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.. in Science of The Total Environment. 2022;843:156964.
doi:10.1016/j.scitotenv.2022.156964 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Đorđević, Ana, Vojnović-Milutinović, Danijela, Jovanović Marić, Jovana, Kirschner, Alexander K T, Farnleitner, Andreas A H, Linke, Rita, Đukić, Aleksandar, Kostić-Vuković, Jovana, Paunović, Momir, "Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters." in Science of The Total Environment, 843 (2022):156964,
https://doi.org/10.1016/j.scitotenv.2022.156964 . .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5727
AB  - U Srbiji se prerađuje manje od 13% otpadnih voda,1 što za posledicu ima
ispuštanje velikih količina neprečišćenih voda u vodotokove. Ravničarske reke
su pod značajnim pritiscima ovih izvora zagađenja, s obzirom da delom ili
čitavim tokom protiču kroz naseljene oblasti, kao i oblasti u kojima se nalaze
industrijska postrojenja. U analizi uticaja zagađenja na akvatične organizme,
oštećenje DNК molekula predstavlja značajan biomarker ranog upozorenja jer
može ukazati na uticaj ksenobiotika na biološke sisteme pre nego što se odrazi
na čitav organizam i/ili populaciju. U skladu sa tim, cilj ovog istraživanja je
procena osetljivosti široko rasprostranjene vrste Alburnus alburnus (uklija)
prilikom detekcije genotoksičnog efekta, kako na prostorno bliskim
lokalitetima na Savi, tako na i prostorno udaljenim na Dunavu, Tisi, Savi i
Velikoj Moravi. Za poređenje lokaliteta korišćen je integrisani odgovor
biomarkera (IBR) koji je objedinio pojedinačne biomarkere: vijabilnost ćelija
krvi, nivo DNК oštećenja ćelija krvi detektovanog u alkalnom komet i
mikronukleus testu i indeks zagađenja metalima/prioritetnim supstancama.
Integrisanjem pojedinačnih odgovora biomarkera u jedinstvenu IBR vrednost
omogućeno je jednostavnije poređenje lokaliteta. Dobijeni rezultati su ukazali da
su odgovori biomarkera uklija specifični za lokalitet. U skladu sa rezultatima,
utvrđeno je da uklija može biti pouzdan bioindikator u diskriminaciji i
udaljenih i prostorno bliskih lokaliteta koji su pod različitim uticajem
zagađenja.
AB  - У Србији се прерађује мање од 13% отпадних вода,1 што за последицу има
испуштање великих количина непречишћених вода у водотокове. Равничарске реке
су под значајним притисцима ових извора загађења, с обзиром да делом или
читавим током протичу кроз насељене области, као и области у којима се налазе
индустријска постројења. У анализи утицаја загађења на акватичне организме,
оштећење ДНК молекула представља значајан биомаркер раног упозорења јер
може указати на утицај ксенобиотика на биолошке системе пре него што се одрази
на читав организам и/или популацију. У складу са тим, циљ овог истраживања је
процена осетљивости широко распрострањене врсте Alburnus alburnus (уклија)
приликом детекције генотоксичног ефекта, како на просторно блиским
локалитетима на Сави, тако на и просторно удаљеним на Дунаву, Тиси, Сави и
Великој Морави. За поређење локалитета коришћен је интегрисани одговор
биомаркера (ИБР) који је објединио појединачне биомаркере: вијабилност ћелија
крви, ниво ДНК оштећења ћелија крви детектованог у алкалном комет и
микронуклеус тесту и индекс загађења металима/приоритетним супстанцама.
Интегрисањем појединачних одговора биомаркера у јединствену ИБР вредност
омогућено је једноставније поређење локалитета. Добијени резултати су указали да
су одговори биомаркера уклија специфични за локалитет. У складу са резултатима,
утврђено је да уклија може бити поуздан биоиндикатор у дискриминацији и
удаљених и просторно блиских локалитета који су под различитим утицајем
загађења.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Ispitivanje osetlјivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.
T1  - Испитивање осетљивости врсте Alburnus alburnus (уклија) у екогенотоксиколошким истраживањима великих равничарских река
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5727
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "U Srbiji se prerađuje manje od 13% otpadnih voda,1 što za posledicu ima
ispuštanje velikih količina neprečišćenih voda u vodotokove. Ravničarske reke
su pod značajnim pritiscima ovih izvora zagađenja, s obzirom da delom ili
čitavim tokom protiču kroz naseljene oblasti, kao i oblasti u kojima se nalaze
industrijska postrojenja. U analizi uticaja zagađenja na akvatične organizme,
oštećenje DNК molekula predstavlja značajan biomarker ranog upozorenja jer
može ukazati na uticaj ksenobiotika na biološke sisteme pre nego što se odrazi
na čitav organizam i/ili populaciju. U skladu sa tim, cilj ovog istraživanja je
procena osetljivosti široko rasprostranjene vrste Alburnus alburnus (uklija)
prilikom detekcije genotoksičnog efekta, kako na prostorno bliskim
lokalitetima na Savi, tako na i prostorno udaljenim na Dunavu, Tisi, Savi i
Velikoj Moravi. Za poređenje lokaliteta korišćen je integrisani odgovor
biomarkera (IBR) koji je objedinio pojedinačne biomarkere: vijabilnost ćelija
krvi, nivo DNК oštećenja ćelija krvi detektovanog u alkalnom komet i
mikronukleus testu i indeks zagađenja metalima/prioritetnim supstancama.
Integrisanjem pojedinačnih odgovora biomarkera u jedinstvenu IBR vrednost
omogućeno je jednostavnije poređenje lokaliteta. Dobijeni rezultati su ukazali da
su odgovori biomarkera uklija specifični za lokalitet. U skladu sa rezultatima,
utvrđeno je da uklija može biti pouzdan bioindikator u diskriminaciji i
udaljenih i prostorno bliskih lokaliteta koji su pod različitim uticajem
zagađenja., У Србији се прерађује мање од 13% отпадних вода,1 што за последицу има
испуштање великих количина непречишћених вода у водотокове. Равничарске реке
су под значајним притисцима ових извора загађења, с обзиром да делом или
читавим током протичу кроз насељене области, као и области у којима се налазе
индустријска постројења. У анализи утицаја загађења на акватичне организме,
оштећење ДНК молекула представља значајан биомаркер раног упозорења јер
може указати на утицај ксенобиотика на биолошке системе пре него што се одрази
на читав организам и/или популацију. У складу са тим, циљ овог истраживања је
процена осетљивости широко распрострањене врсте Alburnus alburnus (уклија)
приликом детекције генотоксичног ефекта, како на просторно блиским
локалитетима на Сави, тако на и просторно удаљеним на Дунаву, Тиси, Сави и
Великој Морави. За поређење локалитета коришћен је интегрисани одговор
биомаркера (ИБР) који је објединио појединачне биомаркере: вијабилност ћелија
крви, ниво ДНК оштећења ћелија крви детектованог у алкалном комет и
микронуклеус тесту и индекс загађења металима/приоритетним супстанцама.
Интегрисањем појединачних одговора биомаркера у јединствену ИБР вредност
омогућено је једноставније поређење локалитета. Добијени резултати су указали да
су одговори биомаркера уклија специфични за локалитет. У складу са резултатима,
утврђено је да уклија може бити поуздан биоиндикатор у дискриминацији и
удаљених и просторно блиских локалитета који су под различитим утицајем
загађења.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Ispitivanje osetlјivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka., Испитивање осетљивости врсте Alburnus alburnus (уклија) у екогенотоксиколошким истраживањима великих равничарских река",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5727"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Ispitivanje osetlјivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 155.
https://hdl.handle.net/21.15107/rcub_ibiss_5727

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Ispitivanje osetlјivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:155.
https://hdl.handle.net/21.15107/rcub_ibiss_5727 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Ispitivanje osetlјivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka." in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):155,
https://hdl.handle.net/21.15107/rcub_ibiss_5727 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4937
AB  - Velika, Zapadna, Južna Morava, kao i Sava, Kolubara i Barička reka su pod značajnim uticajem komunalnih i industrijskih otpadnih voda. Ciljevi studije su procena genotoksičnog potencijala analiziranih lokaliteta i ispitivanje osetljivosti primenjenih biotestova i pristupa istraživanja. Studija slučaja Velika Morava obuhvatila je veliko ispitivano područje na osam lokaliteta na Velikoj, Zapadnoj i Južnoj Moravi, a u studiji Sava ispitivano je tri prostorno bliska lokaliteta koji su pod različitim pritiscima zagađenja. U in silico pristupu na osnovu literaturnih podataka analiziran je toksični/genotoksični potencijal jedinjenja detektovanih u vodi. Ex situ/in vitro pristup obuhvatio je ispitivanje genotoksičnog potencijala pomoću SOS/umuC testa, kao i primenom alkalnog komet testa na HepG2 ćelijskoj liniji. U in situ/in vivo istraživanju analizirano je DNK oštećenje ćelija krvi vrste Alburnus alburnus (uklija) pomoću alkalnog, Fpg-modifikovanog komet testa i mikronukleus testa. Rezultati su pokazali različitu osetljivost zavisno od upotrebljenih testova i pristupa, ali i različit odgovor kod uklija u zavisnosti od tipa zagađenja na lokalitetima. In silico i ex situ/in vitro pristupi su pokazali niži stepen osetljivosti u odnosu na in situ/in vivo testove, a alkalni komet test je pokazao najveći potencijal u diskriminaciji lokaliteta. Utvrđeno je da uklija može biti pouzdan bioindikator u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.
PB  - Novi Sad : Prirodno-matematički fakultet
C3  - 9. memorijalni naučni skup iz zaštite životne sredine "Docent dr Milena Dalmacija": 2022 Mar 31 - Apr 1; Novi Sad, Serbia
T1  - Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama – studije slučaja Velika Morava i Sava
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4937
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "Velika, Zapadna, Južna Morava, kao i Sava, Kolubara i Barička reka su pod značajnim uticajem komunalnih i industrijskih otpadnih voda. Ciljevi studije su procena genotoksičnog potencijala analiziranih lokaliteta i ispitivanje osetljivosti primenjenih biotestova i pristupa istraživanja. Studija slučaja Velika Morava obuhvatila je veliko ispitivano područje na osam lokaliteta na Velikoj, Zapadnoj i Južnoj Moravi, a u studiji Sava ispitivano je tri prostorno bliska lokaliteta koji su pod različitim pritiscima zagađenja. U in silico pristupu na osnovu literaturnih podataka analiziran je toksični/genotoksični potencijal jedinjenja detektovanih u vodi. Ex situ/in vitro pristup obuhvatio je ispitivanje genotoksičnog potencijala pomoću SOS/umuC testa, kao i primenom alkalnog komet testa na HepG2 ćelijskoj liniji. U in situ/in vivo istraživanju analizirano je DNK oštećenje ćelija krvi vrste Alburnus alburnus (uklija) pomoću alkalnog, Fpg-modifikovanog komet testa i mikronukleus testa. Rezultati su pokazali različitu osetljivost zavisno od upotrebljenih testova i pristupa, ali i različit odgovor kod uklija u zavisnosti od tipa zagađenja na lokalitetima. In silico i ex situ/in vitro pristupi su pokazali niži stepen osetljivosti u odnosu na in situ/in vivo testove, a alkalni komet test je pokazao najveći potencijal u diskriminaciji lokaliteta. Utvrđeno je da uklija može biti pouzdan bioindikator u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.",
publisher = "Novi Sad : Prirodno-matematički fakultet",
journal = "9. memorijalni naučni skup iz zaštite životne sredine "Docent dr Milena Dalmacija": 2022 Mar 31 - Apr 1; Novi Sad, Serbia",
title = "Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama – studije slučaja Velika Morava i Sava",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4937"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama – studije slučaja Velika Morava i Sava. in 9. memorijalni naučni skup iz zaštite životne sredine "Docent dr Milena Dalmacija": 2022 Mar 31 - Apr 1; Novi Sad, Serbia
Novi Sad : Prirodno-matematički fakultet..
https://hdl.handle.net/21.15107/rcub_ibiss_4937

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama – studije slučaja Velika Morava i Sava. in 9. memorijalni naučni skup iz zaštite životne sredine "Docent dr Milena Dalmacija": 2022 Mar 31 - Apr 1; Novi Sad, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_4937 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama – studije slučaja Velika Morava i Sava" in 9. memorijalni naučni skup iz zaštite životne sredine "Docent dr Milena Dalmacija": 2022 Mar 31 - Apr 1; Novi Sad, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_4937 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Nikolić, Ivan
AU  - Sunjog, Karolina
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4735
AB  - Metoda nasumično umnožene polimorfne DNK (RAPD) je poslednjih godina našla primenu u ekogenotoksikologiji. U okviru dve studije slučaja na Savi i Dunavu, za RAPD analizu korišćeno je mišićno tkivo jedinki Alburnus alburnus (uklija) uzorkovanih sa ukupno devet lokaliteta. RAPD rezultati istraživanja na Savi ukazuju na grupisanje jedinki sa zagađenih lokaliteta (UB i UK), u odnosu na jedinke sa manje zagađenog lokaliteta, SZ. RAPD rezultati studije slučaja na Dunavu ne pokazuju jasno grupisanje uklija u odnosu na jačinu pritisaka što ukazuje na neophodnost dodatnih analiza.
AB  - In recent years the randomly amplified polymorphic DNA method (RAPD) is frequently used in ecogenotoxicology. Within two case studies on the Sava River and the Danube River, muscle tissue of Alburnus alburnus (bleak) specimens sampled from nine sites was used for RAPD analysis. The RAPD results on the Sava River study indicate the grouping of specimens from polluted sites (UB and UK) as opposed to specimens from the less polluted site, SZ. The RAPD results of the Danube River case study do not show a clear bleak grouping in relation to pollution pressures, indicating that additional analyses are needed.
PB  - Belgarde: Serbian Association for Water Protection
C3  - VODA 2021: Zbornik radova 50. godišnje konferencije o aktuelnim  problemima korišćenja i zaštite voda
T1  - Primena RAPD metode u ekogenotoksikološkim istraživanjima – studija slučaja Sava i Dunav
T1  - Application of RAPD method in ecogenotoxicological research research – the Sava River and the Danube River case studies
SP  - 99
EP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4735
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Nikolić, Ivan and Sunjog, Karolina and Paunović, Momir and Vuković-Gačić, Branka",
year = "2021",
abstract = "Metoda nasumično umnožene polimorfne DNK (RAPD) je poslednjih godina našla primenu u ekogenotoksikologiji. U okviru dve studije slučaja na Savi i Dunavu, za RAPD analizu korišćeno je mišićno tkivo jedinki Alburnus alburnus (uklija) uzorkovanih sa ukupno devet lokaliteta. RAPD rezultati istraživanja na Savi ukazuju na grupisanje jedinki sa zagađenih lokaliteta (UB i UK), u odnosu na jedinke sa manje zagađenog lokaliteta, SZ. RAPD rezultati studije slučaja na Dunavu ne pokazuju jasno grupisanje uklija u odnosu na jačinu pritisaka što ukazuje na neophodnost dodatnih analiza., In recent years the randomly amplified polymorphic DNA method (RAPD) is frequently used in ecogenotoxicology. Within two case studies on the Sava River and the Danube River, muscle tissue of Alburnus alburnus (bleak) specimens sampled from nine sites was used for RAPD analysis. The RAPD results on the Sava River study indicate the grouping of specimens from polluted sites (UB and UK) as opposed to specimens from the less polluted site, SZ. The RAPD results of the Danube River case study do not show a clear bleak grouping in relation to pollution pressures, indicating that additional analyses are needed.",
publisher = "Belgarde: Serbian Association for Water Protection",
journal = "VODA 2021: Zbornik radova 50. godišnje konferencije o aktuelnim  problemima korišćenja i zaštite voda",
title = "Primena RAPD metode u ekogenotoksikološkim istraživanjima – studija slučaja Sava i Dunav, Application of RAPD method in ecogenotoxicological research research – the Sava River and the Danube River case studies",
pages = "99-104",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4735"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Nikolić, I., Sunjog, K., Paunović, M.,& Vuković-Gačić, B.. (2021). Primena RAPD metode u ekogenotoksikološkim istraživanjima – studija slučaja Sava i Dunav. in VODA 2021: Zbornik radova 50. godišnje konferencije o aktuelnim  problemima korišćenja i zaštite voda
Belgarde: Serbian Association for Water Protection., 99-104.
https://hdl.handle.net/21.15107/rcub_ibiss_4735

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Nikolić I, Sunjog K, Paunović M, Vuković-Gačić B. Primena RAPD metode u ekogenotoksikološkim istraživanjima – studija slučaja Sava i Dunav. in VODA 2021: Zbornik radova 50. godišnje konferencije o aktuelnim  problemima korišćenja i zaštite voda. 2021;:99-104.
https://hdl.handle.net/21.15107/rcub_ibiss_4735 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Nikolić, Ivan, Sunjog, Karolina, Paunović, Momir, Vuković-Gačić, Branka, "Primena RAPD metode u ekogenotoksikološkim istraživanjima – studija slučaja Sava i Dunav" in VODA 2021: Zbornik radova 50. godišnje konferencije o aktuelnim  problemima korišćenja i zaštite voda (2021):99-104,
https://hdl.handle.net/21.15107/rcub_ibiss_4735 .

TY  - JOUR
AU  - Oalđe, Mariana
AU  - Kolarević, Stoimir
AU  - Živković, Jelena
AU  - Alimpić Aradski, Ana
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Jelena
AU  - Marin, Petar D.
AU  - Šavikin, Katarina
AU  - Vuković-Gačić, Branka
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - https://pubs.rsc.org/en/content/articlehtml/2021/fo/d1fo00447f
UR  - http://xlink.rsc.org/?DOI=D1FO00447F
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4217
AB  - This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g-1), naringin (up to 5.07 mg g-1) and luteolin-7-O-glucoside (up to 3.54 mg g-1). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.
PB  - Royal Society of Chemistry (RSC)
T2  - Food & Function
T1  - A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro
IS  - 7
VL  - 12
DO  - 10.1039/d1fo00447f
SP  - 3233
EP  - 3245
ER  - 

@article{
author = "Oalđe, Mariana and Kolarević, Stoimir and Živković, Jelena and Alimpić Aradski, Ana and Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Đorđević, Jelena and Marin, Petar D. and Šavikin, Katarina and Vuković-Gačić, Branka and Duletić-Laušević, Sonja",
year = "2021",
abstract = "This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g-1), naringin (up to 5.07 mg g-1) and luteolin-7-O-glucoside (up to 3.54 mg g-1). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Food & Function",
title = "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro",
number = "7",
volume = "12",
doi = "10.1039/d1fo00447f",
pages = "3233-3245"
}

Oalđe, M., Kolarević, S., Živković, J., Alimpić Aradski, A., Jovanović Marić, J., Kračun-Kolarević, M., Đorđević, J., Marin, P. D., Šavikin, K., Vuković-Gačić, B.,& Duletić-Laušević, S.. (2021). A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function
Royal Society of Chemistry (RSC)., 12(7), 3233-3245.
https://doi.org/10.1039/d1fo00447f

Oalđe M, Kolarević S, Živković J, Alimpić Aradski A, Jovanović Marić J, Kračun-Kolarević M, Đorđević J, Marin PD, Šavikin K, Vuković-Gačić B, Duletić-Laušević S. A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function. 2021;12(7):3233-3245.
doi:10.1039/d1fo00447f .

Oalđe, Mariana, Kolarević, Stoimir, Živković, Jelena, Alimpić Aradski, Ana, Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Đorđević, Jelena, Marin, Petar D., Šavikin, Katarina, Vuković-Gačić, Branka, Duletić-Laušević, Sonja, "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro" in Food & Function, 12, no. 7 (2021):3233-3245,
https://doi.org/10.1039/d1fo00447f . .

TY  - JOUR
AU  - Oalđe Pavlović, Mariana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Jovanović Marić, Jovana
AU  - Lunić, Tanja
AU  - Mandić, Marija
AU  - Kračun-Kolarević, Margareta
AU  - Živković, Jelena
AU  - Alimpić Aradski, Ana
AU  - Marin, Petar D.
AU  - Šavikin, Katarina
AU  - Vuković-Gačić, Branka
AU  - Božić Nedeljković, Biljana
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - https://www.mdpi.com/2223-7747/10/11/2306
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4654
AB  - This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
PB  - Basel: MDPI
T2  - Plants
T1  - A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
IS  - 11
VL  - 10
DO  - 10.3390/plants10112306
SP  - 2306
ER  - 

@article{
author = "Oalđe Pavlović, Mariana and Kolarević, Stoimir and Đorđević, Jelena and Jovanović Marić, Jovana and Lunić, Tanja and Mandić, Marija and Kračun-Kolarević, Margareta and Živković, Jelena and Alimpić Aradski, Ana and Marin, Petar D. and Šavikin, Katarina and Vuković-Gačić, Branka and Božić Nedeljković, Biljana and Duletić-Laušević, Sonja",
year = "2021",
abstract = "This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species",
number = "11",
volume = "10",
doi = "10.3390/plants10112306",
pages = "2306"
}

Oalđe Pavlović, M., Kolarević, S., Đorđević, J., Jovanović Marić, J., Lunić, T., Mandić, M., Kračun-Kolarević, M., Živković, J., Alimpić Aradski, A., Marin, P. D., Šavikin, K., Vuković-Gačić, B., Božić Nedeljković, B.,& Duletić-Laušević, S.. (2021). A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants
Basel: MDPI., 10(11), 2306.
https://doi.org/10.3390/plants10112306

Oalđe Pavlović M, Kolarević S, Đorđević J, Jovanović Marić J, Lunić T, Mandić M, Kračun-Kolarević M, Živković J, Alimpić Aradski A, Marin PD, Šavikin K, Vuković-Gačić B, Božić Nedeljković B, Duletić-Laušević S. A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants. 2021;10(11):2306.
doi:10.3390/plants10112306 .

Oalđe Pavlović, Mariana, Kolarević, Stoimir, Đorđević, Jelena, Jovanović Marić, Jovana, Lunić, Tanja, Mandić, Marija, Kračun-Kolarević, Margareta, Živković, Jelena, Alimpić Aradski, Ana, Marin, Petar D., Šavikin, Katarina, Vuković-Gačić, Branka, Božić Nedeljković, Biljana, Duletić-Laušević, Sonja, "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species" in Plants, 10, no. 11 (2021):2306,
https://doi.org/10.3390/plants10112306 . .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Jelena
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4732
AB  - Surface waters in Serbia are under high anthropogenic pressure. One of the major problems is untreated municipal and industrial wastewaters. Unfortunately, Serbia processes only 5 % of wastewaters before discharging. As a consequence, pollutants such as metals and metalloids from industrial wastewaters, pharmaceuticals, compounds from personal care products, etc. directly endure into surface water. Microbial faecal pollution, as an indicator of presence of human or/and animal pathogens, due to health hazard limits water usage for drinking, recreation, irrigation, etc. In regulations, faecal coliforms, with Escherichia coli as dominant representative, are widely used as faecal indicator bacteria. The aim of this study was to investigate the microbiological al water quality in the Republic of Serbia at the sites which are not routinely investigated within the national monitoring program. In this purpose 78 sites situated on canals, mountain springs, as well as lowland rivers were selected. Defined Substrate Technology was used for determination Most Probable Number (MPN) of E. coli using Colilert-18 System. The water classification system, developed for the Danube River (Kavka et al., 2006) was used. 
The results indicated that more than 47 % of selected sites are under critical (21.79 %) or strong (25.64 %) faecal pollution. On the other hand, about 32 % (32.05 %) of sites are little polluted and 20.51 % of sites showed moderate pollution. The increasing levels of faecal pollution detected on sites situated downstream of settlements indicated discharge of untreated municipal wastewaters directly into surface water. In further research, the focus will be placed on the determination of the origin of pollution by the employment of microbial source tracking technique. Obtained data will be used for modelling and predicting the effect of detected contamination on the water quality of the major water bodies in the Republic of Serbia
PB  - Neuburg:  Aueninstitut Neuburg
C3  - 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
T1  - Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4732
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević, Jelena and Paunović, Momir and Vuković-Gačić, Branka",
year = "2021",
abstract = "Surface waters in Serbia are under high anthropogenic pressure. One of the major problems is untreated municipal and industrial wastewaters. Unfortunately, Serbia processes only 5 % of wastewaters before discharging. As a consequence, pollutants such as metals and metalloids from industrial wastewaters, pharmaceuticals, compounds from personal care products, etc. directly endure into surface water. Microbial faecal pollution, as an indicator of presence of human or/and animal pathogens, due to health hazard limits water usage for drinking, recreation, irrigation, etc. In regulations, faecal coliforms, with Escherichia coli as dominant representative, are widely used as faecal indicator bacteria. The aim of this study was to investigate the microbiological al water quality in the Republic of Serbia at the sites which are not routinely investigated within the national monitoring program. In this purpose 78 sites situated on canals, mountain springs, as well as lowland rivers were selected. Defined Substrate Technology was used for determination Most Probable Number (MPN) of E. coli using Colilert-18 System. The water classification system, developed for the Danube River (Kavka et al., 2006) was used. 
The results indicated that more than 47 % of selected sites are under critical (21.79 %) or strong (25.64 %) faecal pollution. On the other hand, about 32 % (32.05 %) of sites are little polluted and 20.51 % of sites showed moderate pollution. The increasing levels of faecal pollution detected on sites situated downstream of settlements indicated discharge of untreated municipal wastewaters directly into surface water. In further research, the focus will be placed on the determination of the origin of pollution by the employment of microbial source tracking technique. Obtained data will be used for modelling and predicting the effect of detected contamination on the water quality of the major water bodies in the Republic of Serbia",
publisher = "Neuburg:  Aueninstitut Neuburg",
journal = "43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin",
title = "Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4732"
}

Jovanović Marić, J., Kolarević, S., Kračun-Kolarević, M., Đorđević, J., Paunović, M.,& Vuković-Gačić, B.. (2021). Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
Neuburg:  Aueninstitut Neuburg., 19.
https://hdl.handle.net/21.15107/rcub_ibiss_4732

Jovanović Marić J, Kolarević S, Kračun-Kolarević M, Đorđević J, Paunović M, Vuković-Gačić B. Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin. 2021;:19.
https://hdl.handle.net/21.15107/rcub_ibiss_4732 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević, Jelena, Paunović, Momir, Vuković-Gačić, Branka, "Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme" in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin (2021):19,
https://hdl.handle.net/21.15107/rcub_ibiss_4732 .

TY  - THES
AU  - Jovanović Marić, Jovana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4420
AB  - Uvođenje metoda uslovljenim efektima (eng. effect based methods), uključujući i genotoksikološke metode, u programe monitoringa i naučna istraživanja na velikim ravničarskim rekama, omogućilo bi sveobuhvatniju procenu stanja ekosistema i odnosa između samog hemijskog i ekološkog statusa. Osnovni ciljevi disertacije su ispitivanje osetljivosti in vitro i in vivo testova u ekogenotoksikologiji i formulisanje smernica za njihovu primenu u istraživanjima velikih ravničarskih reka. Studija je obuhvatila ukupno 17 lokaliteta u slivovima Velike Morave, Save i Dunava. Za procenu genotoksičnog efekta nativnih i koncentrisanih uzoraka rečne vode, in vitro pristup obuhvatio je SOS/umuC test, alkalni komet test na HepG2 i ZFL ćelijama, kao i analizu ćelijskog ciklusa na ZFL ćelijama. In vivo pristup je za procenu genotoksičnosti obuhvatio alkalni, Fpg modifikovani komet test, kao i mikronukleus test na ćelijama krvi Alburnus alburnus (uklija). Dodatno, ispitivana je osetljivost RAPD analize u detekciji genotoksičnog efekta. U in silico pristupu na osnovu literature analiziran je toksični/genotoksični potencijal hemijskih parametara koji su detektovani u vodi i u uklijama, a integrativnim metodama vršena je kvantifikacija pritiska zagađenja. Biotestovi su pokazali različitu osetljivost u detekciji genotoksičnog efekta. U in vitro istraživanju koncentrisani uzorci rečne vode su se pokazali pogodnim za testiranje toksičnosti/genotoksičnosti, a ZFL ćelije kao dobar model sistem. U in vivo istraživanju A. alburnus se pokazao kao pouzdan bioindikator. Najveća osetljivost u detekciji genotoksičnog efekta u ćelijama krvi dobijena je u alkalnom komet testu. In silico analizom ne može se izvršiti potpuna predikcija genotoksičnog efekta ksenobiotika na organizme.
AB  - The implementation of effect based methods, including genotoxicological methods, in monitoring and research programs on large lowland rivers, could provide comprehensive assessment of ecosystem quality and relationship between chemical and ecological status itself. The main objectives of the dissertation are examination the sensitivity of in vitro and in vivo tests in ecogenotoxicology and guidelines formulation for their application in studies of large lowland rivers. The study included a total of 17 sites in the Velika Morava, Sava and Danube River basins. To assess the genotoxic effect of native and concentrated river water samples, the in vitro approach included the SOS/umuC test, alkaline comet test on HepG2 and ZFL cells, and cell cycle analysis on ZFL cells. The in vivo approach for genotoxicity assessment included the alkaline, Fpg modified comet and micronucleus test on Alburnus alburnus (bleak) blood cells. Also, the sensitivity of RAPD analysis in the detection of genotoxic effects was examined. Within in silico approach, based on the literature, the toxic/genotoxic potential of chemical parameters detected in water and bleaks was analyzed while the quantification of pollution pressure was performed by integrative methods. Bioassays showed different sensitivity in detecting the genotoxic effect. Within in vitro study, concentrated river water samples proved to be a suitable for toxicity/genotoxicity testing, and ZFL cells a good model system. Within in vivo research, bleak proved to be a reliable bioindicator. The highest sensitivity in the detection of genotoxic effect in blood cells was obtained in the alkaline comet test. A complete prediction of the genotoxic effect of xenobiotics on organisms cannot be performed by in silico analysis.
PB  - Belgrade: Faculty of Biology, University of Belgrade
T2  - Faculty of Biology, University of Belgrade
T1  - Procena osetljivosti in vitro i in vivo testova u ekogenotokiskologiji i formulisanje smernica za njihovu primenu u istraživanjima na velikim ravničarskim rekama
T1  - Sensitivity assessment of in vitro and in vivo tests in ecogenotoxicology and guidelines formulation for their application in studies of large lowland rivers
SP  - 1
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4420
ER  - 

@phdthesis{
author = "Jovanović Marić, Jovana",
year = "2021",
abstract = "Uvođenje metoda uslovljenim efektima (eng. effect based methods), uključujući i genotoksikološke metode, u programe monitoringa i naučna istraživanja na velikim ravničarskim rekama, omogućilo bi sveobuhvatniju procenu stanja ekosistema i odnosa između samog hemijskog i ekološkog statusa. Osnovni ciljevi disertacije su ispitivanje osetljivosti in vitro i in vivo testova u ekogenotoksikologiji i formulisanje smernica za njihovu primenu u istraživanjima velikih ravničarskih reka. Studija je obuhvatila ukupno 17 lokaliteta u slivovima Velike Morave, Save i Dunava. Za procenu genotoksičnog efekta nativnih i koncentrisanih uzoraka rečne vode, in vitro pristup obuhvatio je SOS/umuC test, alkalni komet test na HepG2 i ZFL ćelijama, kao i analizu ćelijskog ciklusa na ZFL ćelijama. In vivo pristup je za procenu genotoksičnosti obuhvatio alkalni, Fpg modifikovani komet test, kao i mikronukleus test na ćelijama krvi Alburnus alburnus (uklija). Dodatno, ispitivana je osetljivost RAPD analize u detekciji genotoksičnog efekta. U in silico pristupu na osnovu literature analiziran je toksični/genotoksični potencijal hemijskih parametara koji su detektovani u vodi i u uklijama, a integrativnim metodama vršena je kvantifikacija pritiska zagađenja. Biotestovi su pokazali različitu osetljivost u detekciji genotoksičnog efekta. U in vitro istraživanju koncentrisani uzorci rečne vode su se pokazali pogodnim za testiranje toksičnosti/genotoksičnosti, a ZFL ćelije kao dobar model sistem. U in vivo istraživanju A. alburnus se pokazao kao pouzdan bioindikator. Najveća osetljivost u detekciji genotoksičnog efekta u ćelijama krvi dobijena je u alkalnom komet testu. In silico analizom ne može se izvršiti potpuna predikcija genotoksičnog efekta ksenobiotika na organizme., The implementation of effect based methods, including genotoxicological methods, in monitoring and research programs on large lowland rivers, could provide comprehensive assessment of ecosystem quality and relationship between chemical and ecological status itself. The main objectives of the dissertation are examination the sensitivity of in vitro and in vivo tests in ecogenotoxicology and guidelines formulation for their application in studies of large lowland rivers. The study included a total of 17 sites in the Velika Morava, Sava and Danube River basins. To assess the genotoxic effect of native and concentrated river water samples, the in vitro approach included the SOS/umuC test, alkaline comet test on HepG2 and ZFL cells, and cell cycle analysis on ZFL cells. The in vivo approach for genotoxicity assessment included the alkaline, Fpg modified comet and micronucleus test on Alburnus alburnus (bleak) blood cells. Also, the sensitivity of RAPD analysis in the detection of genotoxic effects was examined. Within in silico approach, based on the literature, the toxic/genotoxic potential of chemical parameters detected in water and bleaks was analyzed while the quantification of pollution pressure was performed by integrative methods. Bioassays showed different sensitivity in detecting the genotoxic effect. Within in vitro study, concentrated river water samples proved to be a suitable for toxicity/genotoxicity testing, and ZFL cells a good model system. Within in vivo research, bleak proved to be a reliable bioindicator. The highest sensitivity in the detection of genotoxic effect in blood cells was obtained in the alkaline comet test. A complete prediction of the genotoxic effect of xenobiotics on organisms cannot be performed by in silico analysis.",
publisher = "Belgrade: Faculty of Biology, University of Belgrade",
journal = "Faculty of Biology, University of Belgrade",
title = "Procena osetljivosti in vitro i in vivo testova u ekogenotokiskologiji i formulisanje smernica za njihovu primenu u istraživanjima na velikim ravničarskim rekama, Sensitivity assessment of in vitro and in vivo tests in ecogenotoxicology and guidelines formulation for their application in studies of large lowland rivers",
pages = "1-167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4420"
}

Jovanović Marić, J.. (2021). Procena osetljivosti in vitro i in vivo testova u ekogenotokiskologiji i formulisanje smernica za njihovu primenu u istraživanjima na velikim ravničarskim rekama. in Faculty of Biology, University of Belgrade
Belgrade: Faculty of Biology, University of Belgrade., 1-167.
https://hdl.handle.net/21.15107/rcub_ibiss_4420

Jovanović Marić J. Procena osetljivosti in vitro i in vivo testova u ekogenotokiskologiji i formulisanje smernica za njihovu primenu u istraživanjima na velikim ravničarskim rekama. in Faculty of Biology, University of Belgrade. 2021;:1-167.
https://hdl.handle.net/21.15107/rcub_ibiss_4420 .

Jovanović Marić, Jovana, "Procena osetljivosti in vitro i in vivo testova u ekogenotokiskologiji i formulisanje smernica za njihovu primenu u istraživanjima na velikim ravničarskim rekama" in Faculty of Biology, University of Belgrade (2021):1-167,
https://hdl.handle.net/21.15107/rcub_ibiss_4420 .