TY  - JOUR
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Cingel, Aleksandar
AU  - Ghalawenji, Nabil
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2019
UR  - http://link.springer.com/10.1007/s13205-018-1540-4
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6314937
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3232
AB  - The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.
T2  - 3 Biotech
T1  - Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.
IS  - 1
VL  - 9
DO  - 10.1007/s13205-018-1540-4
SP  - 17
ER  - 

@article{
author = "Trajković, Milena and Antonić Reljin, Dragana and Cingel, Aleksandar and Ghalawenji, Nabil and Subotić, Angelina and Jevremović, Slađana",
year = "2019",
abstract = "The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.",
journal = "3 Biotech",
title = "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.",
number = "1",
volume = "9",
doi = "10.1007/s13205-018-1540-4",
pages = "17"
}

Trajković, M., Antonić Reljin, D., Cingel, A., Ghalawenji, N., Subotić, A.,& Jevremović, S.. (2019). Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech, 9(1), 17.
https://doi.org/10.1007/s13205-018-1540-4

Trajković M, Antonić Reljin D, Cingel A, Ghalawenji N, Subotić A, Jevremović S. Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech. 2019;9(1):17.
doi:10.1007/s13205-018-1540-4 .

Trajković, Milena, Antonić Reljin, Dragana, Cingel, Aleksandar, Ghalawenji, Nabil, Subotić, Angelina, Jevremović, Slađana, "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta." in 3 Biotech, 9, no. 1 (2019):17,
https://doi.org/10.1007/s13205-018-1540-4 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Ghalawenji, Nabil
AU  - Zdravković-Korać, Snežana
PY  - 2019
UR  - http://link.springer.com/10.1007/s11240-019-01595-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3310
AB  - Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)
DO  - 10.1007/s11240-019-01595-6
ER  - 

@article{
author = "Banjac, Nevena and Vinterhalter, Branka and Krstić Milošević, Dijana and Milojević, Jelena and Tubić, Ljiljana and Ghalawenji, Nabil and Zdravković-Korać, Snežana",
year = "2019",
abstract = "Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)",
doi = "10.1007/s11240-019-01595-6"
}

Banjac, N., Vinterhalter, B., Krstić Milošević, D., Milojević, J., Tubić, L., Ghalawenji, N.,& Zdravković-Korać, S.. (2019). Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). in Plant Cell, Tissue and Organ Culture (PCTOC).
https://doi.org/10.1007/s11240-019-01595-6

Banjac N, Vinterhalter B, Krstić Milošević D, Milojević J, Tubić L, Ghalawenji N, Zdravković-Korać S. Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). in Plant Cell, Tissue and Organ Culture (PCTOC). 2019;.
doi:10.1007/s11240-019-01595-6 .

Banjac, Nevena, Vinterhalter, Branka, Krstić Milošević, Dijana, Milojević, Jelena, Tubić, Ljiljana, Ghalawenji, Nabil, Zdravković-Korać, Snežana, "Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)" in Plant Cell, Tissue and Organ Culture (PCTOC) (2019),
https://doi.org/10.1007/s11240-019-01595-6 . .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Bogdanović, Milica
AU  - Subotić, Angelina
AU  - Ghalawenji, Nabil
AU  - Dragićević, Ivana
AU  - Todorović, Slađana
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2539
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/782
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641500087D
UR  - 000377883900017
AB  - Primary and secondary ammonium assimilation is catalyzed by the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway in plants. The Arabidopsis genome contains five cytosolic GS1 genes (GLN1;1 - GLN1;5), one nuclear gene for chloroplastic GS2 isoform (GLN2), two Fd-GOGAT genes (GLU1 and GLU2) and a GLT1 gene coding for NADH-GOGAT. Even though the regulation of GS and GOGAT isoforms has been extensively studied in response to various environmental and metabolic cues in many plant species, little is known about the effects of phytohormones on their regulation. The objective of this study was to investigate the impact of representative plant growth regulators, kinetin (KIN), abscisic acid (ABA), gibberellic acid (GA3) and 2,4-dichlorophenoxyacetic acid (2,4-D), on the expression of A. thaliana GS and GOGAT genes. The obtained results indicate that GS and GOGAT genes are differentially regulated by growth regulators in shoots and roots. KIN and 2,4-D repressed GS and GOGAT expression in roots, with little effect on transcript levels in shoots. KIN affected all tested genes; 2,4-D was apparently more selective and less potent. ABA induced the expression of GLN1;1 and GLU2 in whole seedlings, while GA3 enhanced the expression of all tested genes in shoots, except GLU2. The observed expression patterns are discussed in relation to physiological roles of investigated plant growth regulators and N-assimilating enzymes.
T2  - Archives of Biological Sciences
T1  - Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings
IS  - 2
VL  - 68
DO  - 10.2298/ABS150608087D
SP  - 399
EP  - 404
ER  - 

@article{
author = "Dragićević, Milan and Simonović, Ana and Bogdanović, Milica and Subotić, Angelina and Ghalawenji, Nabil and Dragićević, Ivana and Todorović, Slađana",
year = "2016",
abstract = "Primary and secondary ammonium assimilation is catalyzed by the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway in plants. The Arabidopsis genome contains five cytosolic GS1 genes (GLN1;1 - GLN1;5), one nuclear gene for chloroplastic GS2 isoform (GLN2), two Fd-GOGAT genes (GLU1 and GLU2) and a GLT1 gene coding for NADH-GOGAT. Even though the regulation of GS and GOGAT isoforms has been extensively studied in response to various environmental and metabolic cues in many plant species, little is known about the effects of phytohormones on their regulation. The objective of this study was to investigate the impact of representative plant growth regulators, kinetin (KIN), abscisic acid (ABA), gibberellic acid (GA3) and 2,4-dichlorophenoxyacetic acid (2,4-D), on the expression of A. thaliana GS and GOGAT genes. The obtained results indicate that GS and GOGAT genes are differentially regulated by growth regulators in shoots and roots. KIN and 2,4-D repressed GS and GOGAT expression in roots, with little effect on transcript levels in shoots. KIN affected all tested genes; 2,4-D was apparently more selective and less potent. ABA induced the expression of GLN1;1 and GLU2 in whole seedlings, while GA3 enhanced the expression of all tested genes in shoots, except GLU2. The observed expression patterns are discussed in relation to physiological roles of investigated plant growth regulators and N-assimilating enzymes.",
journal = "Archives of Biological Sciences",
title = "Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings",
number = "2",
volume = "68",
doi = "10.2298/ABS150608087D",
pages = "399-404"
}

Dragićević, M., Simonović, A., Bogdanović, M., Subotić, A., Ghalawenji, N., Dragićević, I.,& Todorović, S.. (2016). Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings. in Archives of Biological Sciences, 68(2), 399-404.
https://doi.org/10.2298/ABS150608087D

Dragićević M, Simonović A, Bogdanović M, Subotić A, Ghalawenji N, Dragićević I, Todorović S. Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings. in Archives of Biological Sciences. 2016;68(2):399-404.
doi:10.2298/ABS150608087D .

Dragićević, Milan, Simonović, Ana, Bogdanović, Milica, Subotić, Angelina, Ghalawenji, Nabil, Dragićević, Ivana, Todorović, Slađana, "Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings" in Archives of Biological Sciences, 68, no. 2 (2016):399-404,
https://doi.org/10.2298/ABS150608087D . .

TY  - JOUR
AU  - Cingel, Aleksandar
AU  - Savic, Jelena
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Ghalawenji, Nabil
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2032
AB  - The evaluation of transgenic plants which is usually carried out under
   controlled conditions in culture rooms and greenhouses can yield
   valuable information about the influence of introduced genes on a
   transgenic plant phenotype. However, an overall assessment of plant
   performance can only be made by testing transgenic plants in the field
   environment. Thus, the effects of pyramided rice cystatin genes OCI and
   OCII on morphological parameters of transgenic potato cv. Desiree,
   Dragacevka and Jelica lines were compared under in vitro, greenhouse,
   and field conditions. All analyzed OC co-expressing transgenic lines
   exhibited normal phenotype, both in vitro and in greenhouse conditions.
   In the field environment, eight of nine OCI/OCII lines were similar to
   the wild-type control plants in their general phenotypic appearance.
   Yield parameters, such as tuber number and tuber weight for these
   phenotypically normal OCI/OCII lines, were also comparable to the
   controls. Only transgenic cv. Jelica line 4 plants exhibited slightly
   reduced growth, atypical leaf morphology and, contrary to the plants of
   other transgenic lines and untransformed controls, failed to flower.
   However, despite the phenotypic and developmental changes under field
   conditions, the OCI/OCII Jelica line 4 did not exhibit a significant
   decrease in tuber yield. Stacking of OCI and OCII genes preserves
   important attributes of the parental lines, confirming that this
   approach could be suitable for improving agronomical traits in potato.
T2  - Archives of Biological Sciences
T1  - Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)
IS  - 3
VL  - 67
DO  - 10.2298/ABS141201058C
SP  - 957
EP  - 964
ER  - 

@article{
author = "Cingel, Aleksandar and Savic, Jelena and Ćosić, Tatjana and Raspor, Martin and Ghalawenji, Nabil and Smigocki, Ann and Ninković, Slavica",
year = "2015",
abstract = "The evaluation of transgenic plants which is usually carried out under
   controlled conditions in culture rooms and greenhouses can yield
   valuable information about the influence of introduced genes on a
   transgenic plant phenotype. However, an overall assessment of plant
   performance can only be made by testing transgenic plants in the field
   environment. Thus, the effects of pyramided rice cystatin genes OCI and
   OCII on morphological parameters of transgenic potato cv. Desiree,
   Dragacevka and Jelica lines were compared under in vitro, greenhouse,
   and field conditions. All analyzed OC co-expressing transgenic lines
   exhibited normal phenotype, both in vitro and in greenhouse conditions.
   In the field environment, eight of nine OCI/OCII lines were similar to
   the wild-type control plants in their general phenotypic appearance.
   Yield parameters, such as tuber number and tuber weight for these
   phenotypically normal OCI/OCII lines, were also comparable to the
   controls. Only transgenic cv. Jelica line 4 plants exhibited slightly
   reduced growth, atypical leaf morphology and, contrary to the plants of
   other transgenic lines and untransformed controls, failed to flower.
   However, despite the phenotypic and developmental changes under field
   conditions, the OCI/OCII Jelica line 4 did not exhibit a significant
   decrease in tuber yield. Stacking of OCI and OCII genes preserves
   important attributes of the parental lines, confirming that this
   approach could be suitable for improving agronomical traits in potato.",
journal = "Archives of Biological Sciences",
title = "Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)",
number = "3",
volume = "67",
doi = "10.2298/ABS141201058C",
pages = "957-964"
}

Cingel, A., Savic, J., Ćosić, T., Raspor, M., Ghalawenji, N., Smigocki, A.,& Ninković, S.. (2015). Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII). in Archives of Biological Sciences, 67(3), 957-964.
https://doi.org/10.2298/ABS141201058C

Cingel A, Savic J, Ćosić T, Raspor M, Ghalawenji N, Smigocki A, Ninković S. Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII). in Archives of Biological Sciences. 2015;67(3):957-964.
doi:10.2298/ABS141201058C .

Cingel, Aleksandar, Savic, Jelena, Ćosić, Tatjana, Raspor, Martin, Ghalawenji, Nabil, Smigocki, Ann, Ninković, Slavica, "Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)" in Archives of Biological Sciences, 67, no. 3 (2015):957-964,
https://doi.org/10.2298/ABS141201058C . .

TY  - JOUR
AU  - Tubić, Ljiljana
AU  - Anackov, Goran
AU  - Milojević, Jelena
AU  - Ghalawenji, Nabil
AU  - Banjac, Nevena
AU  - Igić, Ružica
AU  - Zdravković-Korać, Snežana
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2198
AB  - This study assessed the shoot regeneration capacity of root-tips
   isolated from single seed-derived individual plants, obtained from a
   wild, open-pollinated micropopulation of shallot (Allium ascalonicum).
   Considerable variation was observed in the regeneration capacities of
   individual lines, ranging from 0.93 to 100 \%, and a mean bud number per
   root explant between 0.09 and 20.67. One line was found to be superior,
   and was chosen for protocol optimization, focusing on the 2,4-D/BA
   ratio, duration of the CI phase and light conditions. The application of
   the optimized protocol to other lines, selected for their variable
   regeneration capacities, enhanced the process of regeneration and
   shortened the time required to obtain healthy plantlets, even in
   inferior lines. However, highly responsive lines remained superior,
   indicating that differences at the individual level must not be
   overlooked. The conditions employed in this study may serve as a base to
   facilitate the application of molecular breeding methods in shallot.
T2  - Plant Cell Tissue and Organ Culture
T1  - High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure
IS  - 1
VL  - 118
DO  - 10.1007/s11240-014-0465-9
SP  - 101
EP  - 110
ER  - 

@article{
author = "Tubić, Ljiljana and Anackov, Goran and Milojević, Jelena and Ghalawenji, Nabil and Banjac, Nevena and Igić, Ružica and Zdravković-Korać, Snežana",
year = "2014",
abstract = "This study assessed the shoot regeneration capacity of root-tips
   isolated from single seed-derived individual plants, obtained from a
   wild, open-pollinated micropopulation of shallot (Allium ascalonicum).
   Considerable variation was observed in the regeneration capacities of
   individual lines, ranging from 0.93 to 100 \%, and a mean bud number per
   root explant between 0.09 and 20.67. One line was found to be superior,
   and was chosen for protocol optimization, focusing on the 2,4-D/BA
   ratio, duration of the CI phase and light conditions. The application of
   the optimized protocol to other lines, selected for their variable
   regeneration capacities, enhanced the process of regeneration and
   shortened the time required to obtain healthy plantlets, even in
   inferior lines. However, highly responsive lines remained superior,
   indicating that differences at the individual level must not be
   overlooked. The conditions employed in this study may serve as a base to
   facilitate the application of molecular breeding methods in shallot.",
journal = "Plant Cell Tissue and Organ Culture",
title = "High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure",
number = "1",
volume = "118",
doi = "10.1007/s11240-014-0465-9",
pages = "101-110"
}

Tubić, L., Anackov, G., Milojević, J., Ghalawenji, N., Banjac, N., Igić, R.,& Zdravković-Korać, S.. (2014). High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure. in Plant Cell Tissue and Organ Culture, 118(1), 101-110.
https://doi.org/10.1007/s11240-014-0465-9

Tubić L, Anackov G, Milojević J, Ghalawenji N, Banjac N, Igić R, Zdravković-Korać S. High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure. in Plant Cell Tissue and Organ Culture. 2014;118(1):101-110.
doi:10.1007/s11240-014-0465-9 .

Tubić, Ljiljana, Anackov, Goran, Milojević, Jelena, Ghalawenji, Nabil, Banjac, Nevena, Igić, Ružica, Zdravković-Korać, Snežana, "High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure" in Plant Cell Tissue and Organ Culture, 118, no. 1 (2014):101-110,
https://doi.org/10.1007/s11240-014-0465-9 . .

TY  - JOUR
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Skorić, Marijana
AU  - Ghalawenji, Nabil
AU  - Milutinović, Milica
AU  - Božić, Dragana
AU  - Misic, Danijela
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2307
AB  - Most of the species from the genus Centaurium Hill readily hybridize
   between themselves in natural habitats. Artificial interspecific hybrid
   plants were obtained under in vitro conditions using reciprocal crosses
   between 4 species: Centaurium erythraea, C. littorale, C. maritimum, and
   C. pulchellum. Among 76 crossing experiments performed, 3 types of
   crosses produced seeds and gave 20 interspecific hybrid plants. Nuclear
   DNA quantification, fingerprinting of molecular markers, and
   determination of variation in main secondary metabolite content were
   employed to confirm their hybrid nature. All 3 approaches were capable
   of detecting hybrid plants. RAPD molecular markers proved to be the most
   reliable, always placing the hybrids between their parents in
   dendrograms, while secoiridoid glycoside content also showed
   intermediate profiles in the hybrid plants in relation to the parents.
   Hence, each of the techniques employed can be recommended for
   elucidating the mechanisms of interspecific hybridization in natural
   populations of Centaurium species in further studies. This could help to
   comprehend their breeding system and recognize interspecific
   hybridization as one of the crucial steps in polyploid formation and
   speciation in this genus.
T2  - Turkish Journal of Botany
T1  - Interspecific in vitro hybridization in genus Centaurium and
 identification of hybrids via flow cytometry, RAPD, and secondary
 metabolite profiles
IS  - 1
VL  - 38
DO  - 10.3906/bot-1211-58
SP  - 68
EP  - 79
ER  - 

@article{
author = "Banjanac, Tijana and Šiler, Branislav and Skorić, Marijana and Ghalawenji, Nabil and Milutinović, Milica and Božić, Dragana and Misic, Danijela",
year = "2014",
abstract = "Most of the species from the genus Centaurium Hill readily hybridize
   between themselves in natural habitats. Artificial interspecific hybrid
   plants were obtained under in vitro conditions using reciprocal crosses
   between 4 species: Centaurium erythraea, C. littorale, C. maritimum, and
   C. pulchellum. Among 76 crossing experiments performed, 3 types of
   crosses produced seeds and gave 20 interspecific hybrid plants. Nuclear
   DNA quantification, fingerprinting of molecular markers, and
   determination of variation in main secondary metabolite content were
   employed to confirm their hybrid nature. All 3 approaches were capable
   of detecting hybrid plants. RAPD molecular markers proved to be the most
   reliable, always placing the hybrids between their parents in
   dendrograms, while secoiridoid glycoside content also showed
   intermediate profiles in the hybrid plants in relation to the parents.
   Hence, each of the techniques employed can be recommended for
   elucidating the mechanisms of interspecific hybridization in natural
   populations of Centaurium species in further studies. This could help to
   comprehend their breeding system and recognize interspecific
   hybridization as one of the crucial steps in polyploid formation and
   speciation in this genus.",
journal = "Turkish Journal of Botany",
title = "Interspecific in vitro hybridization in genus Centaurium and
 identification of hybrids via flow cytometry, RAPD, and secondary
 metabolite profiles",
number = "1",
volume = "38",
doi = "10.3906/bot-1211-58",
pages = "68-79"
}

Banjanac, T., Šiler, B., Skorić, M., Ghalawenji, N., Milutinović, M., Božić, D.,& Misic, D.. (2014). Interspecific in vitro hybridization in genus Centaurium and
 identification of hybrids via flow cytometry, RAPD, and secondary
 metabolite profiles. in Turkish Journal of Botany, 38(1), 68-79.
https://doi.org/10.3906/bot-1211-58

Banjanac T, Šiler B, Skorić M, Ghalawenji N, Milutinović M, Božić D, Misic D. Interspecific in vitro hybridization in genus Centaurium and
 identification of hybrids via flow cytometry, RAPD, and secondary
 metabolite profiles. in Turkish Journal of Botany. 2014;38(1):68-79.
doi:10.3906/bot-1211-58 .

Banjanac, Tijana, Šiler, Branislav, Skorić, Marijana, Ghalawenji, Nabil, Milutinović, Milica, Božić, Dragana, Misic, Danijela, "Interspecific in vitro hybridization in genus Centaurium and
 identification of hybrids via flow cytometry, RAPD, and secondary
 metabolite profiles" in Turkish Journal of Botany, 38, no. 1 (2014):68-79,
https://doi.org/10.3906/bot-1211-58 . .

TY  - CONF
AU  - Dragićević, Milan
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Ghalawenji, Nabil
AU  - Simonović, Ana
AU  - Grubišić, Dragoljub
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6177
AB  - Glutamine synthetase (GS, EC 6.3.1.2) is a key enzyme of ammonium assimilation and recycling in plants.
In Arabidopsis, five GLN1 genes encode cytosolic (GS1) isoforms which combine to form, at least nine multimeric GS holoenzymes with overlapping expression. To investigate the role of each isoform, we used respective T-DNA insertion (SALK and SAIL) lines, obtained from The European Arabidopsis Stock Centre. The lines were picked so that the insertion is located in the exons of GLN genes, hopefully preventing the functionality of the transcripts. The plants carrying the T-DNA insertion were selected on kanamycin (for SALK lines carrying the neo gene) or phosphinotricin (for SAIL lines carrying the bar gene). The surviving plants were screened with a three-primer PCR protocol which allows for differentiation of wild type, homozygous and heterozygous plants. For monitoring the transcription of wild type and mutant alleles, the fast, simple and cost-efficient Touch and Go technique was used. So far, homozygous knockout mutants of two high expressed GS isoforms (GLN1;2 and GLN1;3) have been identified.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia
T1  - Screening for glutamine synthetase knockout mutants in Arabidopsis
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6177
ER  - 

@conference{
author = "Dragićević, Milan and Bogdanović, Milica and Todorović, Slađana and Ghalawenji, Nabil and Simonović, Ana and Grubišić, Dragoljub",
year = "2011",
abstract = "Glutamine synthetase (GS, EC 6.3.1.2) is a key enzyme of ammonium assimilation and recycling in plants.
In Arabidopsis, five GLN1 genes encode cytosolic (GS1) isoforms which combine to form, at least nine multimeric GS holoenzymes with overlapping expression. To investigate the role of each isoform, we used respective T-DNA insertion (SALK and SAIL) lines, obtained from The European Arabidopsis Stock Centre. The lines were picked so that the insertion is located in the exons of GLN genes, hopefully preventing the functionality of the transcripts. The plants carrying the T-DNA insertion were selected on kanamycin (for SALK lines carrying the neo gene) or phosphinotricin (for SAIL lines carrying the bar gene). The surviving plants were screened with a three-primer PCR protocol which allows for differentiation of wild type, homozygous and heterozygous plants. For monitoring the transcription of wild type and mutant alleles, the fast, simple and cost-efficient Touch and Go technique was used. So far, homozygous knockout mutants of two high expressed GS isoforms (GLN1;2 and GLN1;3) have been identified.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia",
title = "Screening for glutamine synthetase knockout mutants in Arabidopsis",
pages = "49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6177"
}

Dragićević, M., Bogdanović, M., Todorović, S., Ghalawenji, N., Simonović, A.,& Grubišić, D.. (2011). Screening for glutamine synthetase knockout mutants in Arabidopsis. in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia
Belgrade: Serbian Plant Physiology Society., 49.
https://hdl.handle.net/21.15107/rcub_ibiss_6177

Dragićević M, Bogdanović M, Todorović S, Ghalawenji N, Simonović A, Grubišić D. Screening for glutamine synthetase knockout mutants in Arabidopsis. in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia. 2011;:49.
https://hdl.handle.net/21.15107/rcub_ibiss_6177 .

Dragićević, Milan, Bogdanović, Milica, Todorović, Slađana, Ghalawenji, Nabil, Simonović, Ana, Grubišić, Dragoljub, "Screening for glutamine synthetase knockout mutants in Arabidopsis" in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia (2011):49,
https://hdl.handle.net/21.15107/rcub_ibiss_6177 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Ghalawenji, Nabil
AU  - Grubišić, Dragoljub V.
AU  - Konjević, Radomir M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1722
AB  - A micropropagation protocol was developed for the conservation of critically endangered Serbian perennial Nepeta rtanjensis (Lamiaceae). Rooted shoots were obtained from one-node stem segments and shoot tips on a half-strength Murashige and Skoog (MS) medium without growth regulators. The best pH of the medium for axillary buds induction and for rooting of shoots was found to be at 7 and/or 7.2 respectively. The addition of cytokinins to the culture medium did not significantly stimulated auxillary bud production as compared to the control. On the contrary, on media supplemented with high cytokinin concentrations, only dwarf shoots with rudimentary roots were obtained. All tested concentrations of 6-benzylaminopyrine (BAP) and kinetine (Kn) in combination with 0.1 mg 1(-1) indole-3-acetic acid (IAA) negatively affected the elongation and rooting of shoots. Plants micropropagated on hormone free medium and rooted in vitro were successfully acclimatized in greenhouse and in open field conditions. The result of successful acclimatization was the production of more than 7000 plantlets with normal sexually reproduction. They flowered, fruited and produced seeds which exhibited 47% germination. The survival rate of plants that were transferred to the open field for the acclimatization and exposed to the winter chill was 99%. The reintroduction of N. rtanjensis occurred in May 2004. One thousand plantlets were planted within the historic range of this plant species. The survival rate was also 99%.
T2  - Phyton-Annales Rei Botanicae
T1  - Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia
IS  - 1
VL  - 45
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1722
ER  - 

@article{
author = "Mišić, Danijela and Ghalawenji, Nabil and Grubišić, Dragoljub V. and Konjević, Radomir M",
year = "2005",
abstract = "A micropropagation protocol was developed for the conservation of critically endangered Serbian perennial Nepeta rtanjensis (Lamiaceae). Rooted shoots were obtained from one-node stem segments and shoot tips on a half-strength Murashige and Skoog (MS) medium without growth regulators. The best pH of the medium for axillary buds induction and for rooting of shoots was found to be at 7 and/or 7.2 respectively. The addition of cytokinins to the culture medium did not significantly stimulated auxillary bud production as compared to the control. On the contrary, on media supplemented with high cytokinin concentrations, only dwarf shoots with rudimentary roots were obtained. All tested concentrations of 6-benzylaminopyrine (BAP) and kinetine (Kn) in combination with 0.1 mg 1(-1) indole-3-acetic acid (IAA) negatively affected the elongation and rooting of shoots. Plants micropropagated on hormone free medium and rooted in vitro were successfully acclimatized in greenhouse and in open field conditions. The result of successful acclimatization was the production of more than 7000 plantlets with normal sexually reproduction. They flowered, fruited and produced seeds which exhibited 47% germination. The survival rate of plants that were transferred to the open field for the acclimatization and exposed to the winter chill was 99%. The reintroduction of N. rtanjensis occurred in May 2004. One thousand plantlets were planted within the historic range of this plant species. The survival rate was also 99%.",
journal = "Phyton-Annales Rei Botanicae",
title = "Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia",
number = "1",
volume = "45",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1722"
}

Mišić, D., Ghalawenji, N., Grubišić, D. V.,& Konjević, R. M.. (2005). Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia. in Phyton-Annales Rei Botanicae, 45(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1722

Mišić D, Ghalawenji N, Grubišić DV, Konjević RM. Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia. in Phyton-Annales Rei Botanicae. 2005;45(1):null-20.
https://hdl.handle.net/21.15107/rcub_ibiss_1722 .

Mišić, Danijela, Ghalawenji, Nabil, Grubišić, Dragoljub V., Konjević, Radomir M, "Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia" in Phyton-Annales Rei Botanicae, 45, no. 1 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1722 .