TY  - JOUR
AU  - Albuquerque, Bianca R.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Oliveira, M Beatriz P P
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0963996923001199
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5481
AB  - Chemical constituents and bioactive properties of rambutan (Nephelium lappaceum L.) peel were characterized and heat-/ultrasound-assisted extractions (HAE/UAE) of anthocyanins were optimized by response surface methodology. Five organic acids, the α-, γ-, and δ-tocopherol isoforms, and twenty-five fatty acids (36.8 % oleic acid) were identified, as well as a phenolic profile composed of ellagitannin derivatives, geraniin isomers, ellagic acid, and delphinidin-O derivatives. The extract showed antioxidant activity via lipid peroxidation (IC50 = 2.79 ± 0.03 µg/mL) and oxidative hemolysis (IC50 = 72 ± 2 µg/mL) inhibition, and displayed antibacterial and antifungal properties (MIC ≤ 1 mg/mL). On the other hand, no cytotoxicity was observed in tumor and non-tumor cell lines up to 400 µg/mL. The recovery of anthocyanins was more effective using HAE than UAE, allowing greater yields (16.2 mg/g extract) in just 3 min and using lower ethanol proportions. Overall, rambutan peel could be upcycled into bioactive ingredients and natural colorants for industrial applications.
T2  - Food Research International
T1  - Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins
VL  - 165
DO  - 10.1016/j.foodres.2023.112574
SP  - 112574
ER  - 

@article{
author = "Albuquerque, Bianca R. and Pinela, José and Dias, Maria Inês and Pereira, Carla and Petrović, Jovana and Soković, Marina and Calhelha, Ricardo C. and Oliveira, M Beatriz P P and Ferreira, Isabel C F R and Barros, Lillian",
year = "2023",
abstract = "Chemical constituents and bioactive properties of rambutan (Nephelium lappaceum L.) peel were characterized and heat-/ultrasound-assisted extractions (HAE/UAE) of anthocyanins were optimized by response surface methodology. Five organic acids, the α-, γ-, and δ-tocopherol isoforms, and twenty-five fatty acids (36.8 % oleic acid) were identified, as well as a phenolic profile composed of ellagitannin derivatives, geraniin isomers, ellagic acid, and delphinidin-O derivatives. The extract showed antioxidant activity via lipid peroxidation (IC50 = 2.79 ± 0.03 µg/mL) and oxidative hemolysis (IC50 = 72 ± 2 µg/mL) inhibition, and displayed antibacterial and antifungal properties (MIC ≤ 1 mg/mL). On the other hand, no cytotoxicity was observed in tumor and non-tumor cell lines up to 400 µg/mL. The recovery of anthocyanins was more effective using HAE than UAE, allowing greater yields (16.2 mg/g extract) in just 3 min and using lower ethanol proportions. Overall, rambutan peel could be upcycled into bioactive ingredients and natural colorants for industrial applications.",
journal = "Food Research International",
title = "Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins",
volume = "165",
doi = "10.1016/j.foodres.2023.112574",
pages = "112574"
}

Albuquerque, B. R., Pinela, J., Dias, M. I., Pereira, C., Petrović, J., Soković, M., Calhelha, R. C., Oliveira, M. B. P. P., Ferreira, I. C. F. R.,& Barros, L.. (2023). Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins. in Food Research International, 165, 112574.
https://doi.org/10.1016/j.foodres.2023.112574

Albuquerque BR, Pinela J, Dias MI, Pereira C, Petrović J, Soković M, Calhelha RC, Oliveira MBPP, Ferreira ICFR, Barros L. Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins. in Food Research International. 2023;165:112574.
doi:10.1016/j.foodres.2023.112574 .

Albuquerque, Bianca R., Pinela, José, Dias, Maria Inês, Pereira, Carla, Petrović, Jovana, Soković, Marina, Calhelha, Ricardo C., Oliveira, M Beatriz P P, Ferreira, Isabel C F R, Barros, Lillian, "Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins" in Food Research International, 165 (2023):112574,
https://doi.org/10.1016/j.foodres.2023.112574 . .

TY  - JOUR
AU  - Moreira, Bruna
AU  - Pereira, Eliana
AU  - Finimundy, Tiane C.
AU  - Pinela, José
AU  - Calhelha, Ricardo C.
AU  - Carocho, Márcio
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2022
UR  - http://xlink.rsc.org/?DOI=D2FO00657J
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5120
AB  - Pineapple is a tropical fruit consumed fresh or processed into various food products. However, the peel and crown of this fruit are not industrially exploited, thus generating tons of by-products that represent an economic and environmental concern. In order to promote the upcycling of these by-products, this work aimed to characterize the phenolic profile of its hydroethanolic extracts obtained from pineapple peel and crown leaves and to evaluate their in vitro bioactivity. The HPLC-DAD-ESI/MS analysis allowed the identification of 25 phenolic compounds, including phenolic acids and flavonoids. The antioxidant, cytotoxic, and antimicrobial activity assays highlighted the peel extract as the most promising and, therefore, it was incorporated into a traditional Portuguese pastry cake as a functional ingredient. The nutritional parameters of the developed food were not affected by the incorporation of the extract, but it promoted the antioxidant activity during its shelf-life. Overall, pineapple peel and crown appeared as promising by-products to be exploited by the food industry, which can be achieved through a circular economy approach.
PB  - Cambridge: Royal Society of Chemistry
T2  - Food & Function
T1  - Pineapple by-products as a source of bioactive compounds with potential for industrial food application.
IS  - 19
VL  - 13
DO  - 10.1039/d2fo00657j
SP  - 9959
EP  - 9972
ER  - 

@article{
author = "Moreira, Bruna and Pereira, Eliana and Finimundy, Tiane C. and Pinela, José and Calhelha, Ricardo C. and Carocho, Márcio and Stojković, Dejan and Soković, Marina and Ferreira, Isabel C. F. R. and Caleja, Cristina and Barros, Lillian",
year = "2022",
abstract = "Pineapple is a tropical fruit consumed fresh or processed into various food products. However, the peel and crown of this fruit are not industrially exploited, thus generating tons of by-products that represent an economic and environmental concern. In order to promote the upcycling of these by-products, this work aimed to characterize the phenolic profile of its hydroethanolic extracts obtained from pineapple peel and crown leaves and to evaluate their in vitro bioactivity. The HPLC-DAD-ESI/MS analysis allowed the identification of 25 phenolic compounds, including phenolic acids and flavonoids. The antioxidant, cytotoxic, and antimicrobial activity assays highlighted the peel extract as the most promising and, therefore, it was incorporated into a traditional Portuguese pastry cake as a functional ingredient. The nutritional parameters of the developed food were not affected by the incorporation of the extract, but it promoted the antioxidant activity during its shelf-life. Overall, pineapple peel and crown appeared as promising by-products to be exploited by the food industry, which can be achieved through a circular economy approach.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "Food & Function",
title = "Pineapple by-products as a source of bioactive compounds with potential for industrial food application.",
number = "19",
volume = "13",
doi = "10.1039/d2fo00657j",
pages = "9959-9972"
}

Moreira, B., Pereira, E., Finimundy, T. C., Pinela, J., Calhelha, R. C., Carocho, M., Stojković, D., Soković, M., Ferreira, I. C. F. R., Caleja, C.,& Barros, L.. (2022). Pineapple by-products as a source of bioactive compounds with potential for industrial food application.. in Food & Function
Cambridge: Royal Society of Chemistry., 13(19), 9959-9972.
https://doi.org/10.1039/d2fo00657j

Moreira B, Pereira E, Finimundy TC, Pinela J, Calhelha RC, Carocho M, Stojković D, Soković M, Ferreira ICFR, Caleja C, Barros L. Pineapple by-products as a source of bioactive compounds with potential for industrial food application.. in Food & Function. 2022;13(19):9959-9972.
doi:10.1039/d2fo00657j .

Moreira, Bruna, Pereira, Eliana, Finimundy, Tiane C., Pinela, José, Calhelha, Ricardo C., Carocho, Márcio, Stojković, Dejan, Soković, Marina, Ferreira, Isabel C. F. R., Caleja, Cristina, Barros, Lillian, "Pineapple by-products as a source of bioactive compounds with potential for industrial food application." in Food & Function, 13, no. 19 (2022):9959-9972,
https://doi.org/10.1039/d2fo00657j . .

TY  - JOUR
AU  - Vieira, Dalila M.
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Barros, Lillian
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Barreiro, Maria Filomena
AU  - Ferreira, Isabel C.F.R.
AU  - Castro, Maria Cidalia R.
AU  - Rodrigues, Pedro V.
AU  - Machado, Ana Vera
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/fft2.141
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5435
AB  - Natural extracts have been used in several traditional medicine applications and culinary purposes. Their biological properties (antioxidant and antimicrobial) are due to the presence of several active aromatic compounds. Herein, different natural extracts were evaluated, namely structural and thermal characterization and biological activity, in its natural form and incorporated into a polymeric matrix, to assess their effective potential as additives for active food packaging. While rosemary presented the highest thermal stability with a degradation starting at 327°C, lemon balm extract was the less stable (180°C). Regarding the thiobarbituric acid assay, all extracts presented antioxidant activity, in oxidative hemolysis inhibition; anise, cinnamon, and clove extract did not present any action. Overall, the results demonstrated that leaves (rosemary and green tea) and the rhizome (curcumin) are the plant parts with the best performance. Therefore, extracts from aromatic plants are promising natural additives that can be incorporated into polymeric matrices to produce active food packaging film, increasing products shelf-life.
PB  - Hoboken: Wiley
T2  - Food Frontiers
T1  - Evaluation of plant extracts as an efficient source of additives for active food packaging
IS  - 3
VL  - 3
DO  - 10.1002/fft2.141
SP  - 480
EP  - 488
ER  - 

@article{
author = "Vieira, Dalila M. and Pereira, Carla and Calhelha, Ricardo C. and Barros, Lillian and Petrović, Jovana and Soković, Marina and Barreiro, Maria Filomena and Ferreira, Isabel C.F.R. and Castro, Maria Cidalia R. and Rodrigues, Pedro V. and Machado, Ana Vera",
year = "2022",
abstract = "Natural extracts have been used in several traditional medicine applications and culinary purposes. Their biological properties (antioxidant and antimicrobial) are due to the presence of several active aromatic compounds. Herein, different natural extracts were evaluated, namely structural and thermal characterization and biological activity, in its natural form and incorporated into a polymeric matrix, to assess their effective potential as additives for active food packaging. While rosemary presented the highest thermal stability with a degradation starting at 327°C, lemon balm extract was the less stable (180°C). Regarding the thiobarbituric acid assay, all extracts presented antioxidant activity, in oxidative hemolysis inhibition; anise, cinnamon, and clove extract did not present any action. Overall, the results demonstrated that leaves (rosemary and green tea) and the rhizome (curcumin) are the plant parts with the best performance. Therefore, extracts from aromatic plants are promising natural additives that can be incorporated into polymeric matrices to produce active food packaging film, increasing products shelf-life.",
publisher = "Hoboken: Wiley",
journal = "Food Frontiers",
title = "Evaluation of plant extracts as an efficient source of additives for active food packaging",
number = "3",
volume = "3",
doi = "10.1002/fft2.141",
pages = "480-488"
}

Vieira, D. M., Pereira, C., Calhelha, R. C., Barros, L., Petrović, J., Soković, M., Barreiro, M. F., Ferreira, I. C.F.R., Castro, M. C. R., Rodrigues, P. V.,& Machado, A. V.. (2022). Evaluation of plant extracts as an efficient source of additives for active food packaging. in Food Frontiers
Hoboken: Wiley., 3(3), 480-488.
https://doi.org/10.1002/fft2.141

Vieira DM, Pereira C, Calhelha RC, Barros L, Petrović J, Soković M, Barreiro MF, Ferreira IC, Castro MCR, Rodrigues PV, Machado AV. Evaluation of plant extracts as an efficient source of additives for active food packaging. in Food Frontiers. 2022;3(3):480-488.
doi:10.1002/fft2.141 .

Vieira, Dalila M., Pereira, Carla, Calhelha, Ricardo C., Barros, Lillian, Petrović, Jovana, Soković, Marina, Barreiro, Maria Filomena, Ferreira, Isabel C.F.R., Castro, Maria Cidalia R., Rodrigues, Pedro V., Machado, Ana Vera, "Evaluation of plant extracts as an efficient source of additives for active food packaging" in Food Frontiers, 3, no. 3 (2022):480-488,
https://doi.org/10.1002/fft2.141 . .

TY  - JOUR
AU  - Garcia-Manieri, Jéssica A.A
AU  - Correa, Vanesa Gesser
AU  - Corrêa, Rúbia Carvalho Gomes
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
AU  - Bracht, Adelar
AU  - Peralta, Rosane M.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1878818122002572
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5341
AB  - Leaves and barks of Luehea divaricata (Malvaceae) are used in folk medicine for the treatment of infectious diseases, tumors, rheumatism and diabetes. This work presents in vivo experiments (rats and mice) supported by in vitro and chemical analyses in order to address the above mentioned folk medicinal uses. The phenolic constituents of both aqueous and hydroalcoholic extracts are mainly catechin derivatives (135.4 and 215.9 mg/g, respectively). Both extracts displayed strong antioxidant activity. They also showed antiproliferative activities against tumor cells, nitric oxide synthesis inhibition (indicative of anti-inflammatory activity) and antimicrobial activity against a variety of infectious agents. Paracetamol-induced liver injury in rats, which has a high inflammatory component, was substantially attenuated in animals treated with the hydro-alcoholic extract (200 mg kg−1 day−1). This was deduced from aspartate aminotransferase and alanine aminotransferase measurements in plasma as well as from the hepatic activities of catalase and superoxide dismutase. Both extracts inhibited the pancreatic α-amylase with IC50 values of 11.50 ± 0.05 and 18.70 ± 3.87 μg/mL for the hydro-alcoholic and aqueous extract, respectively. Both extracts inhibited starch digestion in mice at the doses of 200 and 400 mg/kg. In general terms the results confirm several of the vox populi notions about the therapeutic potential of L. divaricata, the antidiabetic action, combined with the hepatoprotective effect, being the most relevant observations. Not less important is the economical perspective for the rural producers which may eventually increase their revenue by selling increasingly valued raw materials derived from L. divaricata based on its therapeutic properties.
PB  - Amsterdam: Elsevier
T2  - Biocatalysis and Agricultural Biotechnology
T1  - Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems
VL  - 45
DO  - 10.1016/j.bcab.2022.102530
SP  - 102530
ER  - 

@article{
author = "Garcia-Manieri, Jéssica A.A and Correa, Vanesa Gesser and Corrêa, Rúbia Carvalho Gomes and Dias, Maria Inês and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R. and Bracht, Adelar and Peralta, Rosane M.",
year = "2022",
abstract = "Leaves and barks of Luehea divaricata (Malvaceae) are used in folk medicine for the treatment of infectious diseases, tumors, rheumatism and diabetes. This work presents in vivo experiments (rats and mice) supported by in vitro and chemical analyses in order to address the above mentioned folk medicinal uses. The phenolic constituents of both aqueous and hydroalcoholic extracts are mainly catechin derivatives (135.4 and 215.9 mg/g, respectively). Both extracts displayed strong antioxidant activity. They also showed antiproliferative activities against tumor cells, nitric oxide synthesis inhibition (indicative of anti-inflammatory activity) and antimicrobial activity against a variety of infectious agents. Paracetamol-induced liver injury in rats, which has a high inflammatory component, was substantially attenuated in animals treated with the hydro-alcoholic extract (200 mg kg−1 day−1). This was deduced from aspartate aminotransferase and alanine aminotransferase measurements in plasma as well as from the hepatic activities of catalase and superoxide dismutase. Both extracts inhibited the pancreatic α-amylase with IC50 values of 11.50 ± 0.05 and 18.70 ± 3.87 μg/mL for the hydro-alcoholic and aqueous extract, respectively. Both extracts inhibited starch digestion in mice at the doses of 200 and 400 mg/kg. In general terms the results confirm several of the vox populi notions about the therapeutic potential of L. divaricata, the antidiabetic action, combined with the hepatoprotective effect, being the most relevant observations. Not less important is the economical perspective for the rural producers which may eventually increase their revenue by selling increasingly valued raw materials derived from L. divaricata based on its therapeutic properties.",
publisher = "Amsterdam: Elsevier",
journal = "Biocatalysis and Agricultural Biotechnology",
title = "Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems",
volume = "45",
doi = "10.1016/j.bcab.2022.102530",
pages = "102530"
}

Garcia-Manieri, J. A.A, Correa, V. G., Corrêa, R. C. G., Dias, M. I., Calhelha, R. C., Ivanov, M., Soković, M., Barros, L., Ferreira, I. C.F.R., Bracht, A.,& Peralta, R. M.. (2022). Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems. in Biocatalysis and Agricultural Biotechnology
Amsterdam: Elsevier., 45, 102530.
https://doi.org/10.1016/j.bcab.2022.102530

Garcia-Manieri JA, Correa VG, Corrêa RCG, Dias MI, Calhelha RC, Ivanov M, Soković M, Barros L, Ferreira IC, Bracht A, Peralta RM. Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems. in Biocatalysis and Agricultural Biotechnology. 2022;45:102530.
doi:10.1016/j.bcab.2022.102530 .

Garcia-Manieri, Jéssica A.A, Correa, Vanesa Gesser, Corrêa, Rúbia Carvalho Gomes, Dias, Maria Inês, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., Bracht, Adelar, Peralta, Rosane M., "Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems" in Biocatalysis and Agricultural Biotechnology, 45 (2022):102530,
https://doi.org/10.1016/j.bcab.2022.102530 . .

TY  - JOUR
AU  - Mascoloti Spréa, Rafael
AU  - Caleja, Cristina
AU  - Pinela, José
AU  - Tiane C., Finimundya
AU  - Calhelha, Ricardo C.
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Prieto, Miguel A.
AU  - Pereira, Eliana
AU  - Amarala, Joana S.
AU  - Barros, Lillian
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5147
AB  - Medicinal and aromatic plants (MAP) have been described as a source of phenolic compounds with potential as antioxidant, antiproliferative and antimicrobial agents. MAP from the Lamiaceae family (Origanum vulgare L., Thymus vulgaris L., Ocimum basilicum L., Salvia officinalis L., Melissa officinalis L., and Matricaria chamomilla L.) were selected to perform a phytochemical and biological screening for their further exploitation as natural bioactive ingredients. The total content of phenolic compounds varied from 184.02 mg/g extract in M. officinalis to 17.97 mg/g extract in M. chamomilla. Caffeic and rosmarinic acids were the main phenolic acids found in the respective hydroalcoholic extracts. The extracts showed a promising antioxidant activity in vitro, being related the phenolic compositions of the extracts, furthermore, all extracts being able to combat lipid peroxidation in TBARS assays with an IC50 under 26 μg/mL, moreover all the plant extract has prevented the oxidative haemolysis in OxHLIA assays at concentrations below 67 μg/mL in a Δt 60 min and under 118 μg/mL for a Δt 120 min. Regarding to the bactericidal and fungicidal action the plant extracts were able to inhibit growth against bacteria associated with food hazards, such as Salmonella typhimurium (MIC < 1) and Listeria monocytogenes (MIC < 1), regarding to fungicidal activity it can be highlighted the MIC values under to 0.25 for Aspergillus versicolor and Trichoderma viride. Overall, the selected Lamiaceae plants stood out as a source of active phytochemicals that can be used by different industries, such as food and cosmetics.
PB  - Amsterdam : Elsevier
T2  - Food Research International
T1  - Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family
VL  - 161
DO  - 10.1016/j.foodres.2022.111875
SP  - 111875
ER  - 

@article{
author = "Mascoloti Spréa, Rafael and Caleja, Cristina and Pinela, José and Tiane C., Finimundya and Calhelha, Ricardo C. and Kostić, Marina and Soković, Marina and Prieto, Miguel A. and Pereira, Eliana and Amarala, Joana S. and Barros, Lillian",
year = "2022",
abstract = "Medicinal and aromatic plants (MAP) have been described as a source of phenolic compounds with potential as antioxidant, antiproliferative and antimicrobial agents. MAP from the Lamiaceae family (Origanum vulgare L., Thymus vulgaris L., Ocimum basilicum L., Salvia officinalis L., Melissa officinalis L., and Matricaria chamomilla L.) were selected to perform a phytochemical and biological screening for their further exploitation as natural bioactive ingredients. The total content of phenolic compounds varied from 184.02 mg/g extract in M. officinalis to 17.97 mg/g extract in M. chamomilla. Caffeic and rosmarinic acids were the main phenolic acids found in the respective hydroalcoholic extracts. The extracts showed a promising antioxidant activity in vitro, being related the phenolic compositions of the extracts, furthermore, all extracts being able to combat lipid peroxidation in TBARS assays with an IC50 under 26 μg/mL, moreover all the plant extract has prevented the oxidative haemolysis in OxHLIA assays at concentrations below 67 μg/mL in a Δt 60 min and under 118 μg/mL for a Δt 120 min. Regarding to the bactericidal and fungicidal action the plant extracts were able to inhibit growth against bacteria associated with food hazards, such as Salmonella typhimurium (MIC < 1) and Listeria monocytogenes (MIC < 1), regarding to fungicidal activity it can be highlighted the MIC values under to 0.25 for Aspergillus versicolor and Trichoderma viride. Overall, the selected Lamiaceae plants stood out as a source of active phytochemicals that can be used by different industries, such as food and cosmetics.",
publisher = "Amsterdam : Elsevier",
journal = "Food Research International",
title = "Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family",
volume = "161",
doi = "10.1016/j.foodres.2022.111875",
pages = "111875"
}

Mascoloti Spréa, R., Caleja, C., Pinela, J., Tiane C., F., Calhelha, R. C., Kostić, M., Soković, M., Prieto, M. A., Pereira, E., Amarala, J. S.,& Barros, L.. (2022). Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family. in Food Research International
Amsterdam : Elsevier., 161, 111875.
https://doi.org/10.1016/j.foodres.2022.111875

Mascoloti Spréa R, Caleja C, Pinela J, Tiane C. F, Calhelha RC, Kostić M, Soković M, Prieto MA, Pereira E, Amarala JS, Barros L. Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family. in Food Research International. 2022;161:111875.
doi:10.1016/j.foodres.2022.111875 .

Mascoloti Spréa, Rafael, Caleja, Cristina, Pinela, José, Tiane C., Finimundya, Calhelha, Ricardo C., Kostić, Marina, Soković, Marina, Prieto, Miguel A., Pereira, Eliana, Amarala, Joana S., Barros, Lillian, "Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family" in Food Research International, 161 (2022):111875,
https://doi.org/10.1016/j.foodres.2022.111875 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Kannan, Abhilash
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C.F.R.
AU  - Sanglard, Dominique
AU  - Soković, Marina
PY  - 2021
UR  - https://doi.org/10.3390/ijms22020483
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4230
AB  - Candida albicans represents one of the most common fungal pathogens. Due to its increasing incidence and the poor efficacy of available antifungals, finding novel antifungal molecules is of great importance. Camphor and eucalyptol are bioactive terpenoid plant constituents and their antifungal properties have been explored previously. In this study, we examined their ability to inhibit the growth of different Candida species in suspension and biofilm, to block hyphal transition along with their impact on genes encoding for efflux pumps (CDR1 and CDR2), ergosterol biosynthesis (ERG11), and cytotoxicity to primary liver cells. Camphor showed excellent antifungal activity with a minimal inhibitory concentration of 0.125–0.35 mg/mL while eucalyptol was active in the range of 2–23 mg/mL. The results showed camphor’s potential to reduce fungal virulence traits, that is, biofilm establishment and hyphae formation. On the other hand, camphor and eucalyptol treatments upregulated CDR1; CDR2 was positively regulated after eucalyptol application while camphor downregulated it. Neither had an impact on ERG11 expression. The beneficial antifungal activities of camphor were achieved with an amount that was non-toxic to porcine liver cells, making it a promising antifungal compound for future development. The antifungal concentration of eucalyptol caused cytotoxic effects and increased expression of efflux pump genes, which suggests that it is an unsuitable antifungal candidate.
PB  - MDPI AG
T2  - International Journal of Molecular Sciences
T1  - Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity
IS  - 2
VL  - 22
DO  - 10.3390/ijms22020483
SP  - 1
EP  - 14
ER  - 

@article{
author = "Ivanov, Marija and Kannan, Abhilash and Stojković, Dejan and Glamočlija, Jasmina and Calhelha, Ricardo C. and Ferreira, Isabel C.F.R. and Sanglard, Dominique and Soković, Marina",
year = "2021",
abstract = "Candida albicans represents one of the most common fungal pathogens. Due to its increasing incidence and the poor efficacy of available antifungals, finding novel antifungal molecules is of great importance. Camphor and eucalyptol are bioactive terpenoid plant constituents and their antifungal properties have been explored previously. In this study, we examined their ability to inhibit the growth of different Candida species in suspension and biofilm, to block hyphal transition along with their impact on genes encoding for efflux pumps (CDR1 and CDR2), ergosterol biosynthesis (ERG11), and cytotoxicity to primary liver cells. Camphor showed excellent antifungal activity with a minimal inhibitory concentration of 0.125–0.35 mg/mL while eucalyptol was active in the range of 2–23 mg/mL. The results showed camphor’s potential to reduce fungal virulence traits, that is, biofilm establishment and hyphae formation. On the other hand, camphor and eucalyptol treatments upregulated CDR1; CDR2 was positively regulated after eucalyptol application while camphor downregulated it. Neither had an impact on ERG11 expression. The beneficial antifungal activities of camphor were achieved with an amount that was non-toxic to porcine liver cells, making it a promising antifungal compound for future development. The antifungal concentration of eucalyptol caused cytotoxic effects and increased expression of efflux pump genes, which suggests that it is an unsuitable antifungal candidate.",
publisher = "MDPI AG",
journal = "International Journal of Molecular Sciences",
title = "Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity",
number = "2",
volume = "22",
doi = "10.3390/ijms22020483",
pages = "1-14"
}

Ivanov, M., Kannan, A., Stojković, D., Glamočlija, J., Calhelha, R. C., Ferreira, I. C.F.R., Sanglard, D.,& Soković, M.. (2021). Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity. in International Journal of Molecular Sciences
MDPI AG., 22(2), 1-14.
https://doi.org/10.3390/ijms22020483

Ivanov M, Kannan A, Stojković D, Glamočlija J, Calhelha RC, Ferreira IC, Sanglard D, Soković M. Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity. in International Journal of Molecular Sciences. 2021;22(2):1-14.
doi:10.3390/ijms22020483 .

Ivanov, Marija, Kannan, Abhilash, Stojković, Dejan, Glamočlija, Jasmina, Calhelha, Ricardo C., Ferreira, Isabel C.F.R., Sanglard, Dominique, Soković, Marina, "Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity" in International Journal of Molecular Sciences, 22, no. 2 (2021):1-14,
https://doi.org/10.3390/ijms22020483 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Bancessi, Aducabe
AU  - Pinela, José
AU  - Inês Dias, Maria
AU  - Liberal, Ângela
AU  - Calhelha, Ricardo C.
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - CATARINO, Luís
AU  - C.F.R. Ferreira, Isabel
AU  - Barros, Lillian
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33038772
UR  - https://radar.ibiss.bg.ac.rs/123456789/3913
AB  - Moringa oleifera is an edible medicinal plant used to fight malnutrition in Africa. In this study, M. oleifera flowers, fruits and seeds from Guinea-Bissau were characterized for their nutritional composition and hydroethanolic and aqueous extracts were prepared to investigate the phenolic profiles and bioactivities. Seeds presented higher levels of proteins (~31 g/100 g dw), fat (~26 g/100 g dw) and flavan-3-ol derivatives, while carbohydrates, proteins, citric acid, and glycosylated flavonoids were abundant in fruits and flowers, these last samples also being rich in α-tocopherol (~18 mg/100 g dw). Some of the identified polyphenols had never been described in M. oleifera. In general, hydroethanolic extracts contained more polyphenols and were more active against lipid peroxidation, NO production, and tumour cells growth. Significant antimicrobial effects against the tested bacteria and fungi strains were displayed by both hydroethanolic and aqueous extracts. The M. oleifera potential to fight malnutrition and health issues was highlighted.
PB  - Elsevier BV
T2  - Food Chemistry
T1  - Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa)
IS  - Pt 1
VL  - 341
DO  - 10.1016/j.foodchem.2020.128229
SP  - 128229
ER  - 

@article{
author = "Fernandes, Ângela and Bancessi, Aducabe and Pinela, José and Inês Dias, Maria and Liberal, Ângela and Calhelha, Ricardo C. and Ćirić, Ana and Soković, Marina and CATARINO, Luís and C.F.R. Ferreira, Isabel and Barros, Lillian",
year = "2021",
abstract = "Moringa oleifera is an edible medicinal plant used to fight malnutrition in Africa. In this study, M. oleifera flowers, fruits and seeds from Guinea-Bissau were characterized for their nutritional composition and hydroethanolic and aqueous extracts were prepared to investigate the phenolic profiles and bioactivities. Seeds presented higher levels of proteins (~31 g/100 g dw), fat (~26 g/100 g dw) and flavan-3-ol derivatives, while carbohydrates, proteins, citric acid, and glycosylated flavonoids were abundant in fruits and flowers, these last samples also being rich in α-tocopherol (~18 mg/100 g dw). Some of the identified polyphenols had never been described in M. oleifera. In general, hydroethanolic extracts contained more polyphenols and were more active against lipid peroxidation, NO production, and tumour cells growth. Significant antimicrobial effects against the tested bacteria and fungi strains were displayed by both hydroethanolic and aqueous extracts. The M. oleifera potential to fight malnutrition and health issues was highlighted.",
publisher = "Elsevier BV",
journal = "Food Chemistry",
title = "Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa)",
number = "Pt 1",
volume = "341",
doi = "10.1016/j.foodchem.2020.128229",
pages = "128229"
}

Fernandes, Â., Bancessi, A., Pinela, J., Inês Dias, M., Liberal, Â., Calhelha, R. C., Ćirić, A., Soković, M., CATARINO, L., C.F.R. Ferreira, I.,& Barros, L.. (2021). Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa). in Food Chemistry
Elsevier BV., 341(Pt 1), 128229.
https://doi.org/10.1016/j.foodchem.2020.128229

Fernandes Â, Bancessi A, Pinela J, Inês Dias M, Liberal Â, Calhelha RC, Ćirić A, Soković M, CATARINO L, C.F.R. Ferreira I, Barros L. Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa). in Food Chemistry. 2021;341(Pt 1):128229.
doi:10.1016/j.foodchem.2020.128229 .

Fernandes, Ângela, Bancessi, Aducabe, Pinela, José, Inês Dias, Maria, Liberal, Ângela, Calhelha, Ricardo C., Ćirić, Ana, Soković, Marina, CATARINO, Luís, C.F.R. Ferreira, Isabel, Barros, Lillian, "Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa)" in Food Chemistry, 341, no. Pt 1 (2021):128229,
https://doi.org/10.1016/j.foodchem.2020.128229 . .

TY  - JOUR
AU  - Silva, Gabriel F. Pantuzza
AU  - Pereira, Eliana
AU  - Melgar, Bruno
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Pereira, Carla
AU  - Abreu, Rui M. V.
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2076-3417/11/1/151
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4094
AB  - Consumers are very concerned with following a healthy diet, along with some precautions that may influence environmental impact. Solanum melongena L. is one of the most consumed vegetables due to its excellent nutritional value and antioxidant action. Associated with its high consumption, considerable amounts of agro-food wastes are produced. This work targets the valorization of this matrix, through the use of its bio-residues to study the obtention of coloring pigments, applying innovative technologies. Its nutritional value, chemical composition, and bioactive potential were evaluated, and the ultrasound-assisted extraction to obtain coloring pigments of high industrial interest was optimized. Considering the results, low contents of fat and carbohydrates and energy value were evident, as well as the presence of compounds of interest (free sugars, organic acids, unsaturated fatty acids, and phenolic acids). In addition, the antioxidant and antimicrobial potential was detected. Response surface methodology was performed to optimize the extraction of natural pigments, showing a concentration of 11.9 mg/g of anthocyanins/g of extract, applying optimal conditions of time, solvent, and solid/liquid ratio of 0.5 min, 68.2% (v/v) and 5 g/L, respectively. S. melongena proved to be a good source of bioactive compounds and natural pigments, which can generate great interest in the food industry.
PB  - MDPI AG
T2  - Applied Sciences
T1  - Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies
IS  - 1
VL  - 11
DO  - 10.3390/app11010151
SP  - 151
ER  - 

@article{
author = "Silva, Gabriel F. Pantuzza and Pereira, Eliana and Melgar, Bruno and Stojković, Dejan and Soković, Marina and Calhelha, Ricardo C. and Pereira, Carla and Abreu, Rui M. V. and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Consumers are very concerned with following a healthy diet, along with some precautions that may influence environmental impact. Solanum melongena L. is one of the most consumed vegetables due to its excellent nutritional value and antioxidant action. Associated with its high consumption, considerable amounts of agro-food wastes are produced. This work targets the valorization of this matrix, through the use of its bio-residues to study the obtention of coloring pigments, applying innovative technologies. Its nutritional value, chemical composition, and bioactive potential were evaluated, and the ultrasound-assisted extraction to obtain coloring pigments of high industrial interest was optimized. Considering the results, low contents of fat and carbohydrates and energy value were evident, as well as the presence of compounds of interest (free sugars, organic acids, unsaturated fatty acids, and phenolic acids). In addition, the antioxidant and antimicrobial potential was detected. Response surface methodology was performed to optimize the extraction of natural pigments, showing a concentration of 11.9 mg/g of anthocyanins/g of extract, applying optimal conditions of time, solvent, and solid/liquid ratio of 0.5 min, 68.2% (v/v) and 5 g/L, respectively. S. melongena proved to be a good source of bioactive compounds and natural pigments, which can generate great interest in the food industry.",
publisher = "MDPI AG",
journal = "Applied Sciences",
title = "Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies",
number = "1",
volume = "11",
doi = "10.3390/app11010151",
pages = "151"
}

Silva, G. F. P., Pereira, E., Melgar, B., Stojković, D., Soković, M., Calhelha, R. C., Pereira, C., Abreu, R. M. V., Ferreira, I. C. F. R.,& Barros, L.. (2021). Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies. in Applied Sciences
MDPI AG., 11(1), 151.
https://doi.org/10.3390/app11010151

Silva GFP, Pereira E, Melgar B, Stojković D, Soković M, Calhelha RC, Pereira C, Abreu RMV, Ferreira ICFR, Barros L. Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies. in Applied Sciences. 2021;11(1):151.
doi:10.3390/app11010151 .

Silva, Gabriel F. Pantuzza, Pereira, Eliana, Melgar, Bruno, Stojković, Dejan, Soković, Marina, Calhelha, Ricardo C., Pereira, Carla, Abreu, Rui M. V., Ferreira, Isabel C. F. R., Barros, Lillian, "Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies" in Applied Sciences, 11, no. 1 (2021):151,
https://doi.org/10.3390/app11010151 . .

TY  - JOUR
AU  - Ribeiro, Ana S.
AU  - Costa, Sofia M.
AU  - Ferreira, Diana P.
AU  - Calhelha, Ricardo C.
AU  - Barros, Lillian
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ferreira, Isabel C.F.R.
AU  - Fangueiro, Raul
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4095
AB  - The combination of biodegradable fibers at nanoscale with plant-based extracts is attracting increasing attention to produce wound dressing systems. In this work, nanofibers based on chitosan (CS), poly(ethylene oxide) (PEO), cellulose nanocrystals (CNC) and acacia plant-based extract were developed by electrospinning. Firstly, the polymeric formulations and electrospinning parameters were optimized, resulting in nanofibers with average diameters of 80 nm. CNC were successfully introduced into the optimized CS/PEO blend and the membranes were characterized by FESEM, ATR-FTIR, TGA, XRD, WVTR and WCA. The CNC incorporation improved the nanofibers' physical integrity, morphology, diameters, water vapor transmission rate and thermal properties. After acacia introduction into the best CS/PEO/CNC system, the antibacterial effect was relatively maintained while the antifungal activity was enhanced for some fungi, demonstrating its great effect against a wide range of microorganisms, which is crucial to prevent or treat infections. All the developed systems exhibited absence of cytotoxicity in non-tumor cells, suggesting their biocompatibility. Finally, a continuous release of the acacia extract was observed for 24 h, showing its prolonged action, which contributes to the healing process while reduces the frequency of dressing's replacement. Overall, the developed nanofibers are very promising to act as localized drug delivery systems for wound care applications.
PB  - Elsevier B.V.
T2  - Reactive and Functional Polymers
T1  - Chitosan/nanocellulose electrospun fibers with enhanced antibacterial and antifungal activity for wound dressing applications
VL  - 159
DO  - 10.1016/j.reactfunctpolym.2020.104808
SP  - 104808
ER  - 

@article{
author = "Ribeiro, Ana S. and Costa, Sofia M. and Ferreira, Diana P. and Calhelha, Ricardo C. and Barros, Lillian and Stojković, Dejan and Soković, Marina and Ferreira, Isabel C.F.R. and Fangueiro, Raul",
year = "2021",
abstract = "The combination of biodegradable fibers at nanoscale with plant-based extracts is attracting increasing attention to produce wound dressing systems. In this work, nanofibers based on chitosan (CS), poly(ethylene oxide) (PEO), cellulose nanocrystals (CNC) and acacia plant-based extract were developed by electrospinning. Firstly, the polymeric formulations and electrospinning parameters were optimized, resulting in nanofibers with average diameters of 80 nm. CNC were successfully introduced into the optimized CS/PEO blend and the membranes were characterized by FESEM, ATR-FTIR, TGA, XRD, WVTR and WCA. The CNC incorporation improved the nanofibers' physical integrity, morphology, diameters, water vapor transmission rate and thermal properties. After acacia introduction into the best CS/PEO/CNC system, the antibacterial effect was relatively maintained while the antifungal activity was enhanced for some fungi, demonstrating its great effect against a wide range of microorganisms, which is crucial to prevent or treat infections. All the developed systems exhibited absence of cytotoxicity in non-tumor cells, suggesting their biocompatibility. Finally, a continuous release of the acacia extract was observed for 24 h, showing its prolonged action, which contributes to the healing process while reduces the frequency of dressing's replacement. Overall, the developed nanofibers are very promising to act as localized drug delivery systems for wound care applications.",
publisher = "Elsevier B.V.",
journal = "Reactive and Functional Polymers",
title = "Chitosan/nanocellulose electrospun fibers with enhanced antibacterial and antifungal activity for wound dressing applications",
volume = "159",
doi = "10.1016/j.reactfunctpolym.2020.104808",
pages = "104808"
}

Ribeiro, A. S., Costa, S. M., Ferreira, D. P., Calhelha, R. C., Barros, L., Stojković, D., Soković, M., Ferreira, I. C.F.R.,& Fangueiro, R.. (2021). Chitosan/nanocellulose electrospun fibers with enhanced antibacterial and antifungal activity for wound dressing applications. in Reactive and Functional Polymers
Elsevier B.V.., 159, 104808.
https://doi.org/10.1016/j.reactfunctpolym.2020.104808

Ribeiro AS, Costa SM, Ferreira DP, Calhelha RC, Barros L, Stojković D, Soković M, Ferreira IC, Fangueiro R. Chitosan/nanocellulose electrospun fibers with enhanced antibacterial and antifungal activity for wound dressing applications. in Reactive and Functional Polymers. 2021;159:104808.
doi:10.1016/j.reactfunctpolym.2020.104808 .

Ribeiro, Ana S., Costa, Sofia M., Ferreira, Diana P., Calhelha, Ricardo C., Barros, Lillian, Stojković, Dejan, Soković, Marina, Ferreira, Isabel C.F.R., Fangueiro, Raul, "Chitosan/nanocellulose electrospun fibers with enhanced antibacterial and antifungal activity for wound dressing applications" in Reactive and Functional Polymers, 159 (2021):104808,
https://doi.org/10.1016/j.reactfunctpolym.2020.104808 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Kannan, Abhilash
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C. F. R.
AU  - Sanglard, Dominique
AU  - Soković, Marina
PY  - 2021
UR  - https://www.mdpi.com/1424-8247/14/1/27
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4097
AB  - Due to the high incidence of fungal infections worldwide, there is an increasing demand for the development of novel therapeutic approaches. A wide range of natural products has been extensively studied, with considerable focus on flavonoids. The antifungal capacity of selected flavones (luteolin, apigenin), flavonols (quercetin), and their glycosylated derivatives (quercitrin, isoquercitrin, rutin, and apigetrin) along with their impact on genes encoding efflux pumps (CDR1) and ergosterol biosynthesis enzyme (ERG11) has been the subject of this study. Cytotoxicity of flavonoids towards primary liver cells has also been addressed. Luteolin, quercitrin, isoquercitrin, and rutin inhibited growth of Candida albicans with the minimal inhibitory concentration of 37.5 µg/mL. The application of isoquercitrin has reduced C. albicans biofilm establishing capacities for 76%, and hyphal formation by yeast. In vitro treatment with apigenin, apigetrin, and quercitrin has downregulated CDR1. Contrary to rutin and apigenin, isoquercitrin has upregulated ERG11. Except apigetrin and quercitrin (90 µg/mL and 73 µg/mL, respectively inhibited 50% of the net cell growth), the examined flavonoids did not exhibit cytotoxicity. The reduction of both fungal virulence and expression of antifungal resistance-linked genes was the most pronounced for apigenin and apigetrin; these results indicate flavonoids’ indispensable capacity for further development as part of an anticandidal therapy or prevention strategy.
PB  - MDPI AG
T2  - Pharmaceuticals
T1  - Flavones, Flavonols, and Glycosylated Derivatives—Impact on Candida albicans Growth and Virulence, Expression of CDR1 and ERG11, Cytotoxicity
IS  - 1
VL  - 14
DO  - 10.3390/ph14010027
SP  - 27
ER  - 

@article{
author = "Ivanov, Marija and Kannan, Abhilash and Stojković, Dejan and Glamočlija, Jasmina and Calhelha, Ricardo C. and Ferreira, Isabel C. F. R. and Sanglard, Dominique and Soković, Marina",
year = "2021",
abstract = "Due to the high incidence of fungal infections worldwide, there is an increasing demand for the development of novel therapeutic approaches. A wide range of natural products has been extensively studied, with considerable focus on flavonoids. The antifungal capacity of selected flavones (luteolin, apigenin), flavonols (quercetin), and their glycosylated derivatives (quercitrin, isoquercitrin, rutin, and apigetrin) along with their impact on genes encoding efflux pumps (CDR1) and ergosterol biosynthesis enzyme (ERG11) has been the subject of this study. Cytotoxicity of flavonoids towards primary liver cells has also been addressed. Luteolin, quercitrin, isoquercitrin, and rutin inhibited growth of Candida albicans with the minimal inhibitory concentration of 37.5 µg/mL. The application of isoquercitrin has reduced C. albicans biofilm establishing capacities for 76%, and hyphal formation by yeast. In vitro treatment with apigenin, apigetrin, and quercitrin has downregulated CDR1. Contrary to rutin and apigenin, isoquercitrin has upregulated ERG11. Except apigetrin and quercitrin (90 µg/mL and 73 µg/mL, respectively inhibited 50% of the net cell growth), the examined flavonoids did not exhibit cytotoxicity. The reduction of both fungal virulence and expression of antifungal resistance-linked genes was the most pronounced for apigenin and apigetrin; these results indicate flavonoids’ indispensable capacity for further development as part of an anticandidal therapy or prevention strategy.",
publisher = "MDPI AG",
journal = "Pharmaceuticals",
title = "Flavones, Flavonols, and Glycosylated Derivatives—Impact on Candida albicans Growth and Virulence, Expression of CDR1 and ERG11, Cytotoxicity",
number = "1",
volume = "14",
doi = "10.3390/ph14010027",
pages = "27"
}

Ivanov, M., Kannan, A., Stojković, D., Glamočlija, J., Calhelha, R. C., Ferreira, I. C. F. R., Sanglard, D.,& Soković, M.. (2021). Flavones, Flavonols, and Glycosylated Derivatives—Impact on Candida albicans Growth and Virulence, Expression of CDR1 and ERG11, Cytotoxicity. in Pharmaceuticals
MDPI AG., 14(1), 27.
https://doi.org/10.3390/ph14010027

Ivanov M, Kannan A, Stojković D, Glamočlija J, Calhelha RC, Ferreira ICFR, Sanglard D, Soković M. Flavones, Flavonols, and Glycosylated Derivatives—Impact on Candida albicans Growth and Virulence, Expression of CDR1 and ERG11, Cytotoxicity. in Pharmaceuticals. 2021;14(1):27.
doi:10.3390/ph14010027 .

Ivanov, Marija, Kannan, Abhilash, Stojković, Dejan, Glamočlija, Jasmina, Calhelha, Ricardo C., Ferreira, Isabel C. F. R., Sanglard, Dominique, Soković, Marina, "Flavones, Flavonols, and Glycosylated Derivatives—Impact on Candida albicans Growth and Virulence, Expression of CDR1 and ERG11, Cytotoxicity" in Pharmaceuticals, 14, no. 1 (2021):27,
https://doi.org/10.3390/ph14010027 . .

TY  - JOUR
AU  - García-Pérez, Pascual
AU  - Ayuso, Manuel
AU  - Lozano-Milo, Eva
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Ivanov, Marija
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
AU  - Gallego, Pedro P.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4207
AB  - Bryophyllum constitutes a subgenus within the genus Kalanchoe that contains several plant species used in traditional medicine worldwide for the treatment of several diseases. However, little is known about the phytoconstituents of Bryophyllum spp. and previous reports have pointed at their low in Planta concentrations of bioactive compounds. In this work, we take advantage of plant in vitro culture for the study of the phenolic compounds found in the aerial parts of Bryophyllum spp. and their associated bioactivities. Our results show that the induction of nutritional stress leads to an improved accumulation of phenolic compounds, mainly flavonols and anthocyanins, represented by myricetin and malvidin glycosides, respectively. This effect is mainly found for B. × houghtonii, whose hydroethanolic extracts promoted the highest antioxidant, cytotoxic and anti-inflammatory activities. In the case of cytotoxic activity, Bryophyllum extracts showed an enhanced activity against the MCF-7 human breast carcinoma cell line. Meanwhile, extracts from B. daigremontianum promoted a wide range of effectiveness against different bacterial and fungal strains. This study is committed to shed light about the phytochemical potential associated to this unexplored subgenus, with the aim of considering Bryophyllum spp. as a valuable source of bioactive compounds for their exploitation in food, cosmetic and pharmaceutical industries.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants
VL  - 162
DO  - 10.1016/j.indcrop.2021.113241
SP  - 113241
ER  - 

@article{
author = "García-Pérez, Pascual and Ayuso, Manuel and Lozano-Milo, Eva and Pereira, Carla and Dias, Maria Inês and Ivanov, Marija and Calhelha, Ricardo C. and Soković, Marina and Ferreira, Isabel C.F.R. and Barros, Lillian and Gallego, Pedro P.",
year = "2021",
abstract = "Bryophyllum constitutes a subgenus within the genus Kalanchoe that contains several plant species used in traditional medicine worldwide for the treatment of several diseases. However, little is known about the phytoconstituents of Bryophyllum spp. and previous reports have pointed at their low in Planta concentrations of bioactive compounds. In this work, we take advantage of plant in vitro culture for the study of the phenolic compounds found in the aerial parts of Bryophyllum spp. and their associated bioactivities. Our results show that the induction of nutritional stress leads to an improved accumulation of phenolic compounds, mainly flavonols and anthocyanins, represented by myricetin and malvidin glycosides, respectively. This effect is mainly found for B. × houghtonii, whose hydroethanolic extracts promoted the highest antioxidant, cytotoxic and anti-inflammatory activities. In the case of cytotoxic activity, Bryophyllum extracts showed an enhanced activity against the MCF-7 human breast carcinoma cell line. Meanwhile, extracts from B. daigremontianum promoted a wide range of effectiveness against different bacterial and fungal strains. This study is committed to shed light about the phytochemical potential associated to this unexplored subgenus, with the aim of considering Bryophyllum spp. as a valuable source of bioactive compounds for their exploitation in food, cosmetic and pharmaceutical industries.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants",
volume = "162",
doi = "10.1016/j.indcrop.2021.113241",
pages = "113241"
}

García-Pérez, P., Ayuso, M., Lozano-Milo, E., Pereira, C., Dias, M. I., Ivanov, M., Calhelha, R. C., Soković, M., Ferreira, I. C.F.R., Barros, L.,& Gallego, P. P.. (2021). Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants. in Industrial Crops and Products
Elsevier B.V.., 162, 113241.
https://doi.org/10.1016/j.indcrop.2021.113241

García-Pérez P, Ayuso M, Lozano-Milo E, Pereira C, Dias MI, Ivanov M, Calhelha RC, Soković M, Ferreira IC, Barros L, Gallego PP. Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants. in Industrial Crops and Products. 2021;162:113241.
doi:10.1016/j.indcrop.2021.113241 .

García-Pérez, Pascual, Ayuso, Manuel, Lozano-Milo, Eva, Pereira, Carla, Dias, Maria Inês, Ivanov, Marija, Calhelha, Ricardo C., Soković, Marina, Ferreira, Isabel C.F.R., Barros, Lillian, Gallego, Pedro P., "Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants" in Industrial Crops and Products, 162 (2021):113241,
https://doi.org/10.1016/j.indcrop.2021.113241 . .

TY  - JOUR
AU  - Albuquerque, Bianca R.
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Oliveira, M. Beatriz P. P.
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2304-8158/10/4/700
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4218
AB  - Fruit bio-residues can be interesting for the recovery of bioactive molecules, such as phenolic compounds, tocopherols, vitamins, among others. These compounds can be targeted at the food industry and used for the development of functional foods or as food additives. In some cases, fruit epicarps are converted into by-products with non-commercial value, and generally, these fruit parts have a higher content in bioactive compounds than the fruit pulp. From this perspective, S. odorifera, a Brazilian fruit, has an inedible epicarp that could be explored to obtain biological compounds. Therefore, the aims of this study were to evaluate the chemical composition and the antioxidant, anti-proliferative, anti-inflammatory, and antimicrobial bioactivities of this by-product. S. odorifera epicarp showed a total of four organic acids, four phenolic compounds, highlighting the high concentration of anthocyanins (24 ± 1 mg/g dry weight (dw)) and high content of tocopherols (366 ± 2 mg/100 g dw). The hydroethanolic extract showed considerable antioxidant activity (EC50 values of 48.2 ± 0.5 and 27 ± 1 µg/mL for TBARS and OxHLIA assays, respectively), as also antibacterial and antifungal activities (minimal inhibitory concentrations (MICs) ≤ 2.2 mg/mL). The results obtained in this study suggest that Sicana odorifera epicarp represents a reliable option for the development of novel natural-based colorants with functional/bioactive proprieties.
PB  - MDPI AG
T2  - Foods
T1  - Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties
IS  - 4
VL  - 10
DO  - 10.3390/foods10040700
SP  - 700
ER  - 

@article{
author = "Albuquerque, Bianca R. and Dias, Maria Inês and Pereira, Carla and Petrović, Jovana and Soković, Marina and Calhelha, Ricardo C. and Oliveira, M. Beatriz P. P. and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Fruit bio-residues can be interesting for the recovery of bioactive molecules, such as phenolic compounds, tocopherols, vitamins, among others. These compounds can be targeted at the food industry and used for the development of functional foods or as food additives. In some cases, fruit epicarps are converted into by-products with non-commercial value, and generally, these fruit parts have a higher content in bioactive compounds than the fruit pulp. From this perspective, S. odorifera, a Brazilian fruit, has an inedible epicarp that could be explored to obtain biological compounds. Therefore, the aims of this study were to evaluate the chemical composition and the antioxidant, anti-proliferative, anti-inflammatory, and antimicrobial bioactivities of this by-product. S. odorifera epicarp showed a total of four organic acids, four phenolic compounds, highlighting the high concentration of anthocyanins (24 ± 1 mg/g dry weight (dw)) and high content of tocopherols (366 ± 2 mg/100 g dw). The hydroethanolic extract showed considerable antioxidant activity (EC50 values of 48.2 ± 0.5 and 27 ± 1 µg/mL for TBARS and OxHLIA assays, respectively), as also antibacterial and antifungal activities (minimal inhibitory concentrations (MICs) ≤ 2.2 mg/mL). The results obtained in this study suggest that Sicana odorifera epicarp represents a reliable option for the development of novel natural-based colorants with functional/bioactive proprieties.",
publisher = "MDPI AG",
journal = "Foods",
title = "Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties",
number = "4",
volume = "10",
doi = "10.3390/foods10040700",
pages = "700"
}

Albuquerque, B. R., Dias, M. I., Pereira, C., Petrović, J., Soković, M., Calhelha, R. C., Oliveira, M. B. P. P., Ferreira, I. C. F. R.,& Barros, L.. (2021). Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties. in Foods
MDPI AG., 10(4), 700.
https://doi.org/10.3390/foods10040700

Albuquerque BR, Dias MI, Pereira C, Petrović J, Soković M, Calhelha RC, Oliveira MBPP, Ferreira ICFR, Barros L. Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties. in Foods. 2021;10(4):700.
doi:10.3390/foods10040700 .

Albuquerque, Bianca R., Dias, Maria Inês, Pereira, Carla, Petrović, Jovana, Soković, Marina, Calhelha, Ricardo C., Oliveira, M. Beatriz P. P., Ferreira, Isabel C. F. R., Barros, Lillian, "Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties" in Foods, 10, no. 4 (2021):700,
https://doi.org/10.3390/foods10040700 . .

TY  - JOUR
AU  - Pires Jr, Eleomar de O
AU  - Pereira, Eliana
AU  - Carocho, Márcio
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Garcia, Carolina C.
AU  - Ferreira, Isabel C. F. R.
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4382
AB  - Flowers of the genus Impatiens are classified as edible; however, their inclusion in the human
diet is not yet a common practice. Its attractive colours have stirred great interest by the food industry.
In this sense, rose (BP) and orange (BO) I. balsamina flowers were nutritionally studied, followed by
an in-depth chemical study profile. The non-anthocyanin and anthocyanin profiles of extracts of
both flower varieties were also determined by high-performance liquid chromatography coupled
to a diode array and mass spectrometry detector (HPLC-DAD-ESI/MS). The results demonstrated
that both varieties presented significant amounts of phenolic compounds, having identified nine
non-anthocyanin compounds and 14 anthocyanin compounds. BP extract stood out in its bioactive
properties (antioxidant and antimicrobial potential) and was selected for incorporation in “bombocas”
filling. Its performance as a colouring ingredient was compared with the control formulations (white
filling) and with E163 (anthocyanins) colorant. The incorporation of the natural ingredient did not
cause changes in the chemical and nutritional composition of the product; and although the colour
conferred was lighter than presented by the formulation with E163 (suggesting a more natural aspect),
the higher antioxidant activity could meet the expectations of the current high-demand consumer.
PB  - Basel: MDPI
T2  - International Journal of Environmental Research and Public Health
T1  - Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product
IS  - 17
VL  - 18
DO  - 10.3390/ijerph18179062
SP  - 9062
ER  - 

@article{
author = "Pires Jr, Eleomar de O and Pereira, Eliana and Carocho, Márcio and Pereira, Carla and Dias, Maria Inês and Calhelha, Ricardo and Ćirić, Ana and Soković, Marina and Garcia, Carolina C. and Ferreira, Isabel C. F. R. and Caleja, Cristina and Barros, Lillian",
year = "2021",
abstract = "Flowers of the genus Impatiens are classified as edible; however, their inclusion in the human
diet is not yet a common practice. Its attractive colours have stirred great interest by the food industry.
In this sense, rose (BP) and orange (BO) I. balsamina flowers were nutritionally studied, followed by
an in-depth chemical study profile. The non-anthocyanin and anthocyanin profiles of extracts of
both flower varieties were also determined by high-performance liquid chromatography coupled
to a diode array and mass spectrometry detector (HPLC-DAD-ESI/MS). The results demonstrated
that both varieties presented significant amounts of phenolic compounds, having identified nine
non-anthocyanin compounds and 14 anthocyanin compounds. BP extract stood out in its bioactive
properties (antioxidant and antimicrobial potential) and was selected for incorporation in “bombocas”
filling. Its performance as a colouring ingredient was compared with the control formulations (white
filling) and with E163 (anthocyanins) colorant. The incorporation of the natural ingredient did not
cause changes in the chemical and nutritional composition of the product; and although the colour
conferred was lighter than presented by the formulation with E163 (suggesting a more natural aspect),
the higher antioxidant activity could meet the expectations of the current high-demand consumer.",
publisher = "Basel: MDPI",
journal = "International Journal of Environmental Research and Public Health",
title = "Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product",
number = "17",
volume = "18",
doi = "10.3390/ijerph18179062",
pages = "9062"
}

Pires Jr, E. d. O., Pereira, E., Carocho, M., Pereira, C., Dias, M. I., Calhelha, R., Ćirić, A., Soković, M., Garcia, C. C., Ferreira, I. C. F. R., Caleja, C.,& Barros, L.. (2021). Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product. in International Journal of Environmental Research and Public Health
Basel: MDPI., 18(17), 9062.
https://doi.org/10.3390/ijerph18179062

Pires Jr EDO, Pereira E, Carocho M, Pereira C, Dias MI, Calhelha R, Ćirić A, Soković M, Garcia CC, Ferreira ICFR, Caleja C, Barros L. Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product. in International Journal of Environmental Research and Public Health. 2021;18(17):9062.
doi:10.3390/ijerph18179062 .

Pires Jr, Eleomar de O, Pereira, Eliana, Carocho, Márcio, Pereira, Carla, Dias, Maria Inês, Calhelha, Ricardo, Ćirić, Ana, Soković, Marina, Garcia, Carolina C., Ferreira, Isabel C. F. R., Caleja, Cristina, Barros, Lillian, "Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product" in International Journal of Environmental Research and Public Health, 18, no. 17 (2021):9062,
https://doi.org/10.3390/ijerph18179062 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Calhelha, Ricardo
AU  - Fernandes, Ângela
AU  - Ferreira, Isabel
AU  - Stojković, Dejan
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2021
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0352-51392100096N
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4761
AB  - Chemical composition of the essential oils (EO) of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia, and the absolute of Rosa centifolia and their bioactivity were examined. Major compounds in P. graveolens EO were monoterpene alcohols citronellol, geraniol and linalool; in L. petersonii EO monoterpene aldehydes geranial, neral and citronellal; in C. martiniii var. motia EO monoterpene alcohol geraniol and ester geranyl acetate, while in absolute of R. centifolia aromatic alcohol 2-phenylethanol. The EO of L. petersonii showed the strongest antibacterial while the EO of C. martinii var. motia the strongest antifungal potential. The best biofilm inhibition capacity was observed with R. centifolia absolute. The results of scanning electron microscopy analysis indicated that the EOs of L. petersonii and P. graveolens changed the number and morphology of C. albicans cells. The L. petersonii EO was the most potent toward tumour cells and exhibited the best biological activity. This is first comparative report summarizing efficacy of studied aromatic samples against pathogenic microbes, providing deeper insight into the modes of antimicrobial action, and at the same time describing their cytotoxicity against cell lines.
PB  - Belgrade: Serbian Chemical Socitey
T2  - Journal of the Serbian Chemical Society
T1  - Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute
IS  - 12
VL  - 86
DO  - 10.2298/JSC210729096N
SP  - 1291
EP  - 1303
ER  - 

@article{
author = "Nikolić, Miloš and Marković, Tatjana and Marković, Dejan and Calhelha, Ricardo and Fernandes, Ângela and Ferreira, Isabel and Stojković, Dejan and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2021",
abstract = "Chemical composition of the essential oils (EO) of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia, and the absolute of Rosa centifolia and their bioactivity were examined. Major compounds in P. graveolens EO were monoterpene alcohols citronellol, geraniol and linalool; in L. petersonii EO monoterpene aldehydes geranial, neral and citronellal; in C. martiniii var. motia EO monoterpene alcohol geraniol and ester geranyl acetate, while in absolute of R. centifolia aromatic alcohol 2-phenylethanol. The EO of L. petersonii showed the strongest antibacterial while the EO of C. martinii var. motia the strongest antifungal potential. The best biofilm inhibition capacity was observed with R. centifolia absolute. The results of scanning electron microscopy analysis indicated that the EOs of L. petersonii and P. graveolens changed the number and morphology of C. albicans cells. The L. petersonii EO was the most potent toward tumour cells and exhibited the best biological activity. This is first comparative report summarizing efficacy of studied aromatic samples against pathogenic microbes, providing deeper insight into the modes of antimicrobial action, and at the same time describing their cytotoxicity against cell lines.",
publisher = "Belgrade: Serbian Chemical Socitey",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute",
number = "12",
volume = "86",
doi = "10.2298/JSC210729096N",
pages = "1291-1303"
}

Nikolić, M., Marković, T., Marković, D., Calhelha, R., Fernandes, Â., Ferreira, I., Stojković, D., Ćirić, A., Glamočlija, J.,& Soković, M.. (2021). Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Socitey., 86(12), 1291-1303.
https://doi.org/10.2298/JSC210729096N

Nikolić M, Marković T, Marković D, Calhelha R, Fernandes Â, Ferreira I, Stojković D, Ćirić A, Glamočlija J, Soković M. Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute. in Journal of the Serbian Chemical Society. 2021;86(12):1291-1303.
doi:10.2298/JSC210729096N .

Nikolić, Miloš, Marković, Tatjana, Marković, Dejan, Calhelha, Ricardo, Fernandes, Ângela, Ferreira, Isabel, Stojković, Dejan, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute" in Journal of the Serbian Chemical Society, 86, no. 12 (2021):1291-1303,
https://doi.org/10.2298/JSC210729096N . .

TY  - CONF
AU  - Pires Júnior, Eleomar O.
AU  - Caleja, Cristina
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo
AU  - Garcia, Carolina C.
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4380
AB  - Edible flowers have been described as presenting several health benefits, associated with powerful
bioactive compounds present in their composition [1]. These matrices have also been explored and applied
in several fields, such as in cosmetic, pharmaceutical, but specially in gastronomy, due to the with
increasing demand of the common consumer for safer and healthier food products [2]. The plants from the
genus Impatiens are popularly known for the attractive colours of the petals, and preliminary studies have
demonstrated a high potential bioactivity of these plants [3]. Thus, the present investigation aimed to
characterize the nutritional value (ash, protein, fat, and carbohydrate content, and energy value, by AOAC
methodology) and the phenolic compounds profile (by High-Performance Liquid Chromatography
coupled with a diode array detector and mass spectrometry by electrospray ionization -
HPLC-DAD-ESI/MS) of Impatiens balsamina L. pink petals. Moreover, the antioxidant, antimicrobial,
cytotoxic, and anti-inflammatory evaluation (by the oxidative hemolysis inhibition assay - OxHLIA,
microdilution method with ATCC strains, the sulforhodamine B method in four human tumour cell lines,
and analysis in macrophage cells of rats (RAW 264.7), to inhibit the production of NO, respectively) in
the hydroethanolic extracts was also accessed. Finally, the enriched-coloured extract was applied as a
colorant in a cake filling called "bombocas", and its colorant capacity was compared with an artificial
additive (E163). In the pink petals, proteins stood out as the main macronutrient, and only fructose and
glucose were found in sugars profile. As for the phenolic composition, eighteen compounds were
tentatively identified, five non-anthocyanin compounds (caffeic and coumaric acids, and
eryodictiol-O-hexoside) and ten anthocyanin compounds (mainly acylated O-glycosylated malvidin,
pelargonidin, and peonidin derivatives). In addition, the hydroethanolic extracts demonstrated
anti-inflammatory and cytotoxicity for all cell lines studied, presenting also a remarkable antifungal
activity. Finally, the coloured extract applied in the formulations conferred a more natural colour to the
"bombocas, as also functional properties such as antioxidant activity. Further studies will be performed in
order to optimize the extractions conditions of colorant compounds, requiring also stabilization strategies
for further evaluation of the colorant extract efficacy.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - Chemical and bioactive characterization of Impatiens balsamina L. pink flowers and their application in a portuguese pastry product
SP  - 192
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4380
ER  - 

@conference{
author = "Pires Júnior, Eleomar O. and Caleja, Cristina and Pinela, José and Dias, Maria Inês and Calhelha, Ricardo and Garcia, Carolina C. and Ćirić, Ana and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Edible flowers have been described as presenting several health benefits, associated with powerful
bioactive compounds present in their composition [1]. These matrices have also been explored and applied
in several fields, such as in cosmetic, pharmaceutical, but specially in gastronomy, due to the with
increasing demand of the common consumer for safer and healthier food products [2]. The plants from the
genus Impatiens are popularly known for the attractive colours of the petals, and preliminary studies have
demonstrated a high potential bioactivity of these plants [3]. Thus, the present investigation aimed to
characterize the nutritional value (ash, protein, fat, and carbohydrate content, and energy value, by AOAC
methodology) and the phenolic compounds profile (by High-Performance Liquid Chromatography
coupled with a diode array detector and mass spectrometry by electrospray ionization -
HPLC-DAD-ESI/MS) of Impatiens balsamina L. pink petals. Moreover, the antioxidant, antimicrobial,
cytotoxic, and anti-inflammatory evaluation (by the oxidative hemolysis inhibition assay - OxHLIA,
microdilution method with ATCC strains, the sulforhodamine B method in four human tumour cell lines,
and analysis in macrophage cells of rats (RAW 264.7), to inhibit the production of NO, respectively) in
the hydroethanolic extracts was also accessed. Finally, the enriched-coloured extract was applied as a
colorant in a cake filling called "bombocas", and its colorant capacity was compared with an artificial
additive (E163). In the pink petals, proteins stood out as the main macronutrient, and only fructose and
glucose were found in sugars profile. As for the phenolic composition, eighteen compounds were
tentatively identified, five non-anthocyanin compounds (caffeic and coumaric acids, and
eryodictiol-O-hexoside) and ten anthocyanin compounds (mainly acylated O-glycosylated malvidin,
pelargonidin, and peonidin derivatives). In addition, the hydroethanolic extracts demonstrated
anti-inflammatory and cytotoxicity for all cell lines studied, presenting also a remarkable antifungal
activity. Finally, the coloured extract applied in the formulations conferred a more natural colour to the
"bombocas, as also functional properties such as antioxidant activity. Further studies will be performed in
order to optimize the extractions conditions of colorant compounds, requiring also stabilization strategies
for further evaluation of the colorant extract efficacy.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "Chemical and bioactive characterization of Impatiens balsamina L. pink flowers and their application in a portuguese pastry product",
pages = "192",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4380"
}

Pires Júnior, E. O., Caleja, C., Pinela, J., Dias, M. I., Calhelha, R., Garcia, C. C., Ćirić, A., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Chemical and bioactive characterization of Impatiens balsamina L. pink flowers and their application in a portuguese pastry product. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 192.
https://hdl.handle.net/21.15107/rcub_ibiss_4380

Pires Júnior EO, Caleja C, Pinela J, Dias MI, Calhelha R, Garcia CC, Ćirić A, Soković M, Ferreira ICFR, Barros L. Chemical and bioactive characterization of Impatiens balsamina L. pink flowers and their application in a portuguese pastry product. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:192.
https://hdl.handle.net/21.15107/rcub_ibiss_4380 .

Pires Júnior, Eleomar O., Caleja, Cristina, Pinela, José, Dias, Maria Inês, Calhelha, Ricardo, Garcia, Carolina C., Ćirić, Ana, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "Chemical and bioactive characterization of Impatiens balsamina L. pink flowers and their application in a portuguese pastry product" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):192,
https://hdl.handle.net/21.15107/rcub_ibiss_4380 .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Plexida, Sofia
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikolaos
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/2071-1050/12/20/8511
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3943
AB  - The environmental burden from crop production byproducts is gradually increasing and necessitates the sustainable management of waste towards a circular economy approach. In the present study, three byproducts (cotton ginning waste (CGW), ground hazelnut husks (GHH) and ground peanut husks (GPH)) were evaluated in lettuce cultivation. For this purpose, the tested materials were incorporated in soil at two different rates (25% and 50% of total substrate volume) while a control treatment (no addition of byproducts) was also considered. Fresh weight per plant and total yield was the highest for the GHH50% treatment. The highest fat, protein, carbohydrates and energy content were observed for the CGW25% treatment. Chemical composition also differed among the tested byproducts where CGW25% treatment had the highest total tocopherols, sugars (sucrose, fructose, trehalose and total sugars) and organic acids content. The most abundant fatty acids were α-linolenic, linoleic and palmitic acid in all the tested treatments, while the highest antioxidant activity was observed for the GHH50% treatment. Regarding polyphenols, phenolic acids content was the highest in the GHH treatments, whereas flavonoids were the highest for the CGW25% treatment. No cytotoxicity against the PLP2 non-tumor cell line was observed, whereas only the GPH50% treatment showed moderate efficacy against HeLa, HepG2 and MCF-7 cell lines. The tested extracts also showed moderate antibacterial activities and only the extracts from the CGW50% treatment were more effective than the positive control against Trichoderma viride. In conclusion, the present results showed the great potential of using the tested byproducts as soil amendments for vegetable crops production, since they may improve the nutritional parameters, the chemical profile and the bioactivities of the final product. The suggested alternative use of the tested byproducts not only will increase the added value of crops but will also alleviate the environmental burden from bulky agroindustry byproducts.
PB  - MDPI AG
T2  - Sustainability
T1  - The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production
IS  - 20
VL  - 12
DO  - 10.3390/su12208511
SP  - 8511
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Plexida, Sofia and Pereira, Carla and Dias, Maria Inês and Calhelha, Ricardo and Chrysargyris, Antonios and Tzortzakis, Nikolaos and Petrović, Jovana and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2020",
abstract = "The environmental burden from crop production byproducts is gradually increasing and necessitates the sustainable management of waste towards a circular economy approach. In the present study, three byproducts (cotton ginning waste (CGW), ground hazelnut husks (GHH) and ground peanut husks (GPH)) were evaluated in lettuce cultivation. For this purpose, the tested materials were incorporated in soil at two different rates (25% and 50% of total substrate volume) while a control treatment (no addition of byproducts) was also considered. Fresh weight per plant and total yield was the highest for the GHH50% treatment. The highest fat, protein, carbohydrates and energy content were observed for the CGW25% treatment. Chemical composition also differed among the tested byproducts where CGW25% treatment had the highest total tocopherols, sugars (sucrose, fructose, trehalose and total sugars) and organic acids content. The most abundant fatty acids were α-linolenic, linoleic and palmitic acid in all the tested treatments, while the highest antioxidant activity was observed for the GHH50% treatment. Regarding polyphenols, phenolic acids content was the highest in the GHH treatments, whereas flavonoids were the highest for the CGW25% treatment. No cytotoxicity against the PLP2 non-tumor cell line was observed, whereas only the GPH50% treatment showed moderate efficacy against HeLa, HepG2 and MCF-7 cell lines. The tested extracts also showed moderate antibacterial activities and only the extracts from the CGW50% treatment were more effective than the positive control against Trichoderma viride. In conclusion, the present results showed the great potential of using the tested byproducts as soil amendments for vegetable crops production, since they may improve the nutritional parameters, the chemical profile and the bioactivities of the final product. The suggested alternative use of the tested byproducts not only will increase the added value of crops but will also alleviate the environmental burden from bulky agroindustry byproducts.",
publisher = "MDPI AG",
journal = "Sustainability",
title = "The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production",
number = "20",
volume = "12",
doi = "10.3390/su12208511",
pages = "8511"
}

Petropoulos, S. A., Fernandes, Â., Plexida, S., Pereira, C., Dias, M. I., Calhelha, R., Chrysargyris, A., Tzortzakis, N., Petrović, J., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production. in Sustainability
MDPI AG., 12(20), 8511.
https://doi.org/10.3390/su12208511

Petropoulos SA, Fernandes Â, Plexida S, Pereira C, Dias MI, Calhelha R, Chrysargyris A, Tzortzakis N, Petrović J, Soković M, Ferreira ICFR, Barros L. The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production. in Sustainability. 2020;12(20):8511.
doi:10.3390/su12208511 .

Petropoulos, Spyridon A., Fernandes, Ângela, Plexida, Sofia, Pereira, Carla, Dias, Maria Inês, Calhelha, Ricardo, Chrysargyris, Antonios, Tzortzakis, Nikolaos, Petrović, Jovana, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production" in Sustainability, 12, no. 20 (2020):8511,
https://doi.org/10.3390/su12208511 . .

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Fernandes, Ângela
AU  - Pinela, José
AU  - Calhelha, Ricardo C.
AU  - Glamočlija, Jasmina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/18/4336
UR  - https://radar.ibiss.bg.ac.rs/123456789/3905
AB  - This study explored the biological properties of three wild growing Russula species (R. integra, R. rosea, R. nigricans) from Serbia. Compositional features and antioxidant, antibacterial, antibiofilm, and cytotoxic activities were analyzed. The studied mushroom species were identified as being rich sources of carbohydrates and of low caloric value. Mannitol was the most abundant free sugar and quinic and malic acids the major organic acids detected. The four tocopherol isoforms were found, and polyunsaturated fatty acids were the predominant fat constituents. Regarding phenolic compounds, P-hydroxybenzoic and cinnamic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation and oxidative hemolysis; the highest activity was attributed to the R. nigricans ethanolic extract. This is the first report on the antibacterial and antibiofilm potential of the studied species, with the most promising activity observed towards Streptococcus spp. (0.20–0.78 mg/mL as the minimal inhibitory concentration, MIC). The most promising cytotoxic effect was caused by the R. integra methanolic extract on non-small cell lung cancer cells (NCI-H460). Therefore, due to the observed in vitro bioactive properties, the studied mushrooms arise as a source of functional ingredients with potential to be used in novel nutraceutical and drug formulations, which can be used in the treatment of various diseases and health conditions.
PB  - NLM (Medline)
T2  - Molecules
T1  - Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity
IS  - 18
VL  - 25
DO  - 10.3390/molecules25184336
SP  - 4336
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Fernandes, Ângela and Pinela, José and Calhelha, Ricardo C. and Glamočlija, Jasmina and Barros, Lillian and Ferreira, Isabel C. F. R. and Soković, Marina and Ćirić, Ana",
year = "2020",
abstract = "This study explored the biological properties of three wild growing Russula species (R. integra, R. rosea, R. nigricans) from Serbia. Compositional features and antioxidant, antibacterial, antibiofilm, and cytotoxic activities were analyzed. The studied mushroom species were identified as being rich sources of carbohydrates and of low caloric value. Mannitol was the most abundant free sugar and quinic and malic acids the major organic acids detected. The four tocopherol isoforms were found, and polyunsaturated fatty acids were the predominant fat constituents. Regarding phenolic compounds, P-hydroxybenzoic and cinnamic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation and oxidative hemolysis; the highest activity was attributed to the R. nigricans ethanolic extract. This is the first report on the antibacterial and antibiofilm potential of the studied species, with the most promising activity observed towards Streptococcus spp. (0.20–0.78 mg/mL as the minimal inhibitory concentration, MIC). The most promising cytotoxic effect was caused by the R. integra methanolic extract on non-small cell lung cancer cells (NCI-H460). Therefore, due to the observed in vitro bioactive properties, the studied mushrooms arise as a source of functional ingredients with potential to be used in novel nutraceutical and drug formulations, which can be used in the treatment of various diseases and health conditions.",
publisher = "NLM (Medline)",
journal = "Molecules",
title = "Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity",
number = "18",
volume = "25",
doi = "10.3390/molecules25184336",
pages = "4336"
}

Kostić, M., Ivanov, M., Fernandes, Â., Pinela, J., Calhelha, R. C., Glamočlija, J., Barros, L., Ferreira, I. C. F. R., Soković, M.,& Ćirić, A.. (2020). Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity. in Molecules
NLM (Medline)., 25(18), 4336.
https://doi.org/10.3390/molecules25184336

Kostić M, Ivanov M, Fernandes Â, Pinela J, Calhelha RC, Glamočlija J, Barros L, Ferreira ICFR, Soković M, Ćirić A. Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity. in Molecules. 2020;25(18):4336.
doi:10.3390/molecules25184336 .

Kostić, Marina, Ivanov, Marija, Fernandes, Ângela, Pinela, José, Calhelha, Ricardo C., Glamočlija, Jasmina, Barros, Lillian, Ferreira, Isabel C. F. R., Soković, Marina, Ćirić, Ana, "Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity" in Molecules, 25, no. 18 (2020):4336,
https://doi.org/10.3390/molecules25184336 . .

TY  - JOUR
AU  - Pereira, Eliana
AU  - Cadavez, Vasco
AU  - Barros, Lillian
AU  - Encina-Zelada, Christian
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Gonzales-Barron, Ursula
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3832
AB  - The ingestion of bioactive compounds has revealed health benefits, namely in the prevention and/or treatment of several diseases. This work aims to characterize the phenolic profile of three colour varieties of Chenopodium quinoa Willd. grains (black, red and white), and also evaluate their cytotoxic and antimicrobial activity. All varieties revealed the presence of phenolic compounds, namely, quercetin and kaempferol derivatives. In this study, quinoa grains did not reveal any anti-proliferative capacity in tumour cell lines, and, as expected, they were devoid of any toxicity. All of the analysed extracts possessed antibacterial and antifungal activities (inhibitory and bactericidal/fungicidal) against the microbial strains considered, exhibiting promising values of minimum bactericidal concentrations (mean MBC 0.153–0.916 mg/mL) and minimum fungicidal concentrations (mean MFC 0.211–0.884 mg/mL). Quinoa varieties represent a good source of bioactive compounds, interfering beneficially in the organism, specifically as antimicrobial agents. Thus, these extracts could be used in the development of bioactive ingredients.
PB  - Elsevier Ltd
T2  - Food Research International
T1  - Chenopodium quinoa Willd. (quinoa) grains: A good source of phenolic compounds
VL  - 137
DO  - 10.1016/j.foodres.2020.109574
SP  - 109574
ER  - 

@article{
author = "Pereira, Eliana and Cadavez, Vasco and Barros, Lillian and Encina-Zelada, Christian and Stojković, Dejan and Soković, Marina and Calhelha, Ricardo C. and Gonzales-Barron, Ursula and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "The ingestion of bioactive compounds has revealed health benefits, namely in the prevention and/or treatment of several diseases. This work aims to characterize the phenolic profile of three colour varieties of Chenopodium quinoa Willd. grains (black, red and white), and also evaluate their cytotoxic and antimicrobial activity. All varieties revealed the presence of phenolic compounds, namely, quercetin and kaempferol derivatives. In this study, quinoa grains did not reveal any anti-proliferative capacity in tumour cell lines, and, as expected, they were devoid of any toxicity. All of the analysed extracts possessed antibacterial and antifungal activities (inhibitory and bactericidal/fungicidal) against the microbial strains considered, exhibiting promising values of minimum bactericidal concentrations (mean MBC 0.153–0.916 mg/mL) and minimum fungicidal concentrations (mean MFC 0.211–0.884 mg/mL). Quinoa varieties represent a good source of bioactive compounds, interfering beneficially in the organism, specifically as antimicrobial agents. Thus, these extracts could be used in the development of bioactive ingredients.",
publisher = "Elsevier Ltd",
journal = "Food Research International",
title = "Chenopodium quinoa Willd. (quinoa) grains: A good source of phenolic compounds",
volume = "137",
doi = "10.1016/j.foodres.2020.109574",
pages = "109574"
}

Pereira, E., Cadavez, V., Barros, L., Encina-Zelada, C., Stojković, D., Soković, M., Calhelha, R. C., Gonzales-Barron, U.,& Ferreira, I. C.F.R.. (2020). Chenopodium quinoa Willd. (quinoa) grains: A good source of phenolic compounds. in Food Research International
Elsevier Ltd., 137, 109574.
https://doi.org/10.1016/j.foodres.2020.109574

Pereira E, Cadavez V, Barros L, Encina-Zelada C, Stojković D, Soković M, Calhelha RC, Gonzales-Barron U, Ferreira IC. Chenopodium quinoa Willd. (quinoa) grains: A good source of phenolic compounds. in Food Research International. 2020;137:109574.
doi:10.1016/j.foodres.2020.109574 .

Pereira, Eliana, Cadavez, Vasco, Barros, Lillian, Encina-Zelada, Christian, Stojković, Dejan, Soković, Marina, Calhelha, Ricardo C., Gonzales-Barron, Ursula, Ferreira, Isabel C.F.R., "Chenopodium quinoa Willd. (quinoa) grains: A good source of phenolic compounds" in Food Research International, 137 (2020):109574,
https://doi.org/10.1016/j.foodres.2020.109574 . .

TY  - JOUR
AU  - Ghada, Bejaoui
AU  - Pereira, Eliana
AU  - Pinela, José
AU  - Prieto, Miguel A.
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Zaghdoudi, Khalil
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/14/3203
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32674320
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3830
AB  - The potential of passion fruit (Passiflora edulis Sims) epicarp to produce anthocyanin-based colorants with bioactive properties was evaluated. First, a five-level three-factor factorial design coupled with response surface methodology was implemented to optimize the extraction of anthocyanins from dark purple epicarps. The extraction yield and cyanidin-3-O-glucoside content were used as response criteria. The constructed models were fitted to the experimental data and used to calculate the optimal processing conditions (t = 38 min, T = 20 °C, S = 0% ethanol/water (v/v) acidified with citric acid to pH 3, and RS/L = 50 g/L) that lead to maximum responses (3.4 mg/g dried epicarp and 9 mg/g extract). Then, the antioxidant, antimicrobial, and cytotoxic activities of anthocyanin extracts obtained using the optimized method and a conventional extraction method were evaluated in vitro. The extract obtained by the optimized method revealed a higher bioactivity, in agreement with the higher cyanidin-3-O-glucoside content. This study highlighted the coloring and bioactive potential of a bio-based ingredient recycled from a bio-waste, which promotes a sustainable bioeconomy in the agri-food sector.
PB  - NLM (Medline)
T2  - Molecules (Basel, Switzerland)
T1  - Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.
IS  - 14
VL  - 25
DO  - 10.3390/molecules25143203
SP  - 3203
ER  - 

@article{
author = "Ghada, Bejaoui and Pereira, Eliana and Pinela, José and Prieto, Miguel A. and Pereira, Carla and Calhelha, Ricardo C. and Stojković, Dejan and Soković, Marina and Zaghdoudi, Khalil and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "The potential of passion fruit (Passiflora edulis Sims) epicarp to produce anthocyanin-based colorants with bioactive properties was evaluated. First, a five-level three-factor factorial design coupled with response surface methodology was implemented to optimize the extraction of anthocyanins from dark purple epicarps. The extraction yield and cyanidin-3-O-glucoside content were used as response criteria. The constructed models were fitted to the experimental data and used to calculate the optimal processing conditions (t = 38 min, T = 20 °C, S = 0% ethanol/water (v/v) acidified with citric acid to pH 3, and RS/L = 50 g/L) that lead to maximum responses (3.4 mg/g dried epicarp and 9 mg/g extract). Then, the antioxidant, antimicrobial, and cytotoxic activities of anthocyanin extracts obtained using the optimized method and a conventional extraction method were evaluated in vitro. The extract obtained by the optimized method revealed a higher bioactivity, in agreement with the higher cyanidin-3-O-glucoside content. This study highlighted the coloring and bioactive potential of a bio-based ingredient recycled from a bio-waste, which promotes a sustainable bioeconomy in the agri-food sector.",
publisher = "NLM (Medline)",
journal = "Molecules (Basel, Switzerland)",
title = "Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.",
number = "14",
volume = "25",
doi = "10.3390/molecules25143203",
pages = "3203"
}

Ghada, B., Pereira, E., Pinela, J., Prieto, M. A., Pereira, C., Calhelha, R. C., Stojković, D., Soković, M., Zaghdoudi, K., Barros, L.,& Ferreira, I. C. F. R.. (2020). Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.. in Molecules (Basel, Switzerland)
NLM (Medline)., 25(14), 3203.
https://doi.org/10.3390/molecules25143203

Ghada B, Pereira E, Pinela J, Prieto MA, Pereira C, Calhelha RC, Stojković D, Soković M, Zaghdoudi K, Barros L, Ferreira ICFR. Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.. in Molecules (Basel, Switzerland). 2020;25(14):3203.
doi:10.3390/molecules25143203 .

Ghada, Bejaoui, Pereira, Eliana, Pinela, José, Prieto, Miguel A., Pereira, Carla, Calhelha, Ricardo C., Stojković, Dejan, Soković, Marina, Zaghdoudi, Khalil, Barros, Lillian, Ferreira, Isabel C. F. R., "Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties." in Molecules (Basel, Switzerland), 25, no. 14 (2020):3203,
https://doi.org/10.3390/molecules25143203 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/14/3175
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32664565
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3829
AB  - The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.
PB  - NLM (Medline)
T2  - Molecules (Basel, Switzerland)
T1  - The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.
IS  - 14
VL  - 25
DO  - 10.3390/molecules25143175
SP  - 3175
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C F R and Barros, Lillian",
year = "2020",
abstract = "The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.",
publisher = "NLM (Medline)",
journal = "Molecules (Basel, Switzerland)",
title = "The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.",
number = "14",
volume = "25",
doi = "10.3390/molecules25143175",
pages = "3175"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.. in Molecules (Basel, Switzerland)
NLM (Medline)., 25(14), 3175.
https://doi.org/10.3390/molecules25143175

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Ivanov M, Soković M, Ferreira ICFR, Barros L. The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.. in Molecules (Basel, Switzerland). 2020;25(14):3175.
doi:10.3390/molecules25143175 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Ferreira, Isabel C F R, Barros, Lillian, "The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark." in Molecules (Basel, Switzerland), 25, no. 14 (2020):3175,
https://doi.org/10.3390/molecules25143175 . .

TY  - JOUR
AU  - Gonzales-Barron, Ursula
AU  - Dijkshoorn, Rody
AU  - Maloncy, Maikel
AU  - Finimundy, Tiane
AU  - Calhelha, Ricardo C.
AU  - Pereira, Carla
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Cadavez, Vasco
PY  - 2020
UR  - https://www.mdpi.com/2304-8158/9/5/597
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7278699
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3692
AB  - Although the nutritional profile, bioactivities, and uses of mesquite pod flour from various Prosopis species have been studied, limited research has been conducted on Prosopis pallida (Humb, & Bonpl. Ex Willd.) Kunth mesquite flour. This study aimed to characterize the nutritional quality and bioactive properties of P. pallida pod flour and to assess its technological performance in breadmaking as a partial replacer of white wheat flour. Peruvian P. pallida mesquite flour was found to have an appealing nutritional profile, with high contents of dietary fiber (29.6% dw) and protein (9.5% dw), and low contents of fat (1.0% dw) and carbohydrates (57.6% dw). It is a source of palmitic (12.6%), oleic (35.5%), and linoleic acids (45.8%), α-, β-, and γ- tocopherols, and contains phenolic compounds such as apigenin glycoside derivatives with proven antioxidant capacities. Extracts of P. pallida flour were also found to have antimicrobial and antifungal effects and did not show hepatoxicity. When formulated as a wheat flour replacer, increasing mesquite flour levels yield composite doughs of lower stickiness and extensibility, and composite breads of lower elasticity (p < 0.01). However, up to a level of 10%, mesquite flour significantly increases loaf volume, reduces crumb hardness, and produces a more uniform crumb of small size alveoli (p < 0.01). Considering the purpose of improving the nutritional and technological quality of wheat flour bread, the addition of P. pallida pod flour can be highly recommended.
PB  - MDPI Multidisciplinary Digital Publishing Institute
T2  - Foods (Basel, Switzerland)
T1  - Nutritive and Bioactive Properties of Mesquite (Prosopis pallida) Flour and Its Technological Performance in Breadmaking.
IS  - 5
VL  - 9
DO  - 10.3390/foods9050597
SP  - 597
ER  - 

@article{
author = "Gonzales-Barron, Ursula and Dijkshoorn, Rody and Maloncy, Maikel and Finimundy, Tiane and Calhelha, Ricardo C. and Pereira, Carla and Stojković, Dejan and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian and Cadavez, Vasco",
year = "2020",
abstract = "Although the nutritional profile, bioactivities, and uses of mesquite pod flour from various Prosopis species have been studied, limited research has been conducted on Prosopis pallida (Humb, & Bonpl. Ex Willd.) Kunth mesquite flour. This study aimed to characterize the nutritional quality and bioactive properties of P. pallida pod flour and to assess its technological performance in breadmaking as a partial replacer of white wheat flour. Peruvian P. pallida mesquite flour was found to have an appealing nutritional profile, with high contents of dietary fiber (29.6% dw) and protein (9.5% dw), and low contents of fat (1.0% dw) and carbohydrates (57.6% dw). It is a source of palmitic (12.6%), oleic (35.5%), and linoleic acids (45.8%), α-, β-, and γ- tocopherols, and contains phenolic compounds such as apigenin glycoside derivatives with proven antioxidant capacities. Extracts of P. pallida flour were also found to have antimicrobial and antifungal effects and did not show hepatoxicity. When formulated as a wheat flour replacer, increasing mesquite flour levels yield composite doughs of lower stickiness and extensibility, and composite breads of lower elasticity (p < 0.01). However, up to a level of 10%, mesquite flour significantly increases loaf volume, reduces crumb hardness, and produces a more uniform crumb of small size alveoli (p < 0.01). Considering the purpose of improving the nutritional and technological quality of wheat flour bread, the addition of P. pallida pod flour can be highly recommended.",
publisher = "MDPI Multidisciplinary Digital Publishing Institute",
journal = "Foods (Basel, Switzerland)",
title = "Nutritive and Bioactive Properties of Mesquite (Prosopis pallida) Flour and Its Technological Performance in Breadmaking.",
number = "5",
volume = "9",
doi = "10.3390/foods9050597",
pages = "597"
}

Gonzales-Barron, U., Dijkshoorn, R., Maloncy, M., Finimundy, T., Calhelha, R. C., Pereira, C., Stojković, D., Soković, M., Ferreira, I. C. F. R., Barros, L.,& Cadavez, V.. (2020). Nutritive and Bioactive Properties of Mesquite (Prosopis pallida) Flour and Its Technological Performance in Breadmaking.. in Foods (Basel, Switzerland)
MDPI Multidisciplinary Digital Publishing Institute., 9(5), 597.
https://doi.org/10.3390/foods9050597

Gonzales-Barron U, Dijkshoorn R, Maloncy M, Finimundy T, Calhelha RC, Pereira C, Stojković D, Soković M, Ferreira ICFR, Barros L, Cadavez V. Nutritive and Bioactive Properties of Mesquite (Prosopis pallida) Flour and Its Technological Performance in Breadmaking.. in Foods (Basel, Switzerland). 2020;9(5):597.
doi:10.3390/foods9050597 .

Gonzales-Barron, Ursula, Dijkshoorn, Rody, Maloncy, Maikel, Finimundy, Tiane, Calhelha, Ricardo C., Pereira, Carla, Stojković, Dejan, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, Cadavez, Vasco, "Nutritive and Bioactive Properties of Mesquite (Prosopis pallida) Flour and Its Technological Performance in Breadmaking." in Foods (Basel, Switzerland), 9, no. 5 (2020):597,
https://doi.org/10.3390/foods9050597 . .

TY  - JOUR
AU  - Finimundy, Tiane C.
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Caleja, Cristina
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Stojković, Dejan
AU  - Carvalho, Ana Maria
AU  - Rosa, Eduardo
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/9/2151
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3677
AB  - Several plants have been used for medicinal applications and have been traditionally consumed as decoctions and infusions. Although some herbs are used alone as a beverage, they are often blended in mixtures to maximize their effects. Herein, the nutritional characterization of six infusions from herbal blends was evaluated using the official methods of analysis (AOAC international). A further characterization of the individual phenolic profile was also performed by HPLC-DAD/ESI-MSn, and finally bioactive potential was determined by evaluating the antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities of each blend. The wide variety of plants in each sample led to variability in the results for all analyzed parameters. However, blends containing 15% Laurus nobilis L. and 15% Juglan regia L. in their composition showed higher sugar content and energy contribution; higher concentration of phenolic compounds (phenolic acids and flavonoids); greater antioxidant, cytotoxic, and anti-inflammatory capacity; and also better antimicrobial effects against all the tested bacterial and fungal strains. Further studies will be necessary to evaluate the real synergistic effects that these two species show in the presence of other plants, and to evaluate their potential for application in various food, pharmaceutical, and nutraceutical products as infusion preparations.
PB  - NLM (Medline)
T2  - Molecules
T1  - Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties
IS  - 9
VL  - 25
DO  - 10.3390/molecules25092151
SP  - 2151
ER  - 

@article{
author = "Finimundy, Tiane C. and Pereira, Carla and Dias, Maria Inês and Caleja, Cristina and Calhelha, Ricardo C. and Soković, Marina and Stojković, Dejan and Carvalho, Ana Maria and Rosa, Eduardo and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "Several plants have been used for medicinal applications and have been traditionally consumed as decoctions and infusions. Although some herbs are used alone as a beverage, they are often blended in mixtures to maximize their effects. Herein, the nutritional characterization of six infusions from herbal blends was evaluated using the official methods of analysis (AOAC international). A further characterization of the individual phenolic profile was also performed by HPLC-DAD/ESI-MSn, and finally bioactive potential was determined by evaluating the antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities of each blend. The wide variety of plants in each sample led to variability in the results for all analyzed parameters. However, blends containing 15% Laurus nobilis L. and 15% Juglan regia L. in their composition showed higher sugar content and energy contribution; higher concentration of phenolic compounds (phenolic acids and flavonoids); greater antioxidant, cytotoxic, and anti-inflammatory capacity; and also better antimicrobial effects against all the tested bacterial and fungal strains. Further studies will be necessary to evaluate the real synergistic effects that these two species show in the presence of other plants, and to evaluate their potential for application in various food, pharmaceutical, and nutraceutical products as infusion preparations.",
publisher = "NLM (Medline)",
journal = "Molecules",
title = "Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties",
number = "9",
volume = "25",
doi = "10.3390/molecules25092151",
pages = "2151"
}

Finimundy, T. C., Pereira, C., Dias, M. I., Caleja, C., Calhelha, R. C., Soković, M., Stojković, D., Carvalho, A. M., Rosa, E., Barros, L.,& Ferreira, I. C. F. R.. (2020). Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties. in Molecules
NLM (Medline)., 25(9), 2151.
https://doi.org/10.3390/molecules25092151

Finimundy TC, Pereira C, Dias MI, Caleja C, Calhelha RC, Soković M, Stojković D, Carvalho AM, Rosa E, Barros L, Ferreira ICFR. Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties. in Molecules. 2020;25(9):2151.
doi:10.3390/molecules25092151 .

Finimundy, Tiane C., Pereira, Carla, Dias, Maria Inês, Caleja, Cristina, Calhelha, Ricardo C., Soković, Marina, Stojković, Dejan, Carvalho, Ana Maria, Rosa, Eduardo, Barros, Lillian, Ferreira, Isabel C. F. R., "Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties" in Molecules, 25, no. 9 (2020):2151,
https://doi.org/10.3390/molecules25092151 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Ines
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikolaos
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/9/2204
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3675
AB  - The aim of this report was to study the effect of salinity (control: 2dS/m, S1: 4 dS/m and S2: 6 dS/m) and harvest time (first harvest on 9 May 2018 and second harvest on 19 April 2018) on the growth and the chemical composition of Centaurea raphanina subsp. mixta plants. The plants of the first harvest were used for the plant growth measurements (fresh weight and moisture content of leaves, rosette diameter, number and thickness of leaves), whereas those of the second harvest were not used for these measurements due to the flowering initiation, which made the leaves unmarketable due to their hard texture. The results of our study showed that C. raphanina subsp. mixta plants can be cultivated under mild salinity (S1 treatment) conditions without severe effects on plant growth and yield, since a more severe loss (27.5%) was observed for the S2 treatment. In addition, harvest time proved to be a cost-effective cultivation practice that allows to regulate the quality of the final product, either in edible form (first harvest) or for nutraceutical and pharmaceutical purposes as well as antimicrobial agents in food products. Therefore, the combination of these two agronomic factors showed interesting results in terms of the quality of the final product. In particular, high salinity (S2 treatment) improved the nutritional value by increasing the fat, proteins and carbohydrates contents in the first harvest, as well as the tocopherols and sugars contents (S1 and S2 treatments, respectively) in the second harvest. In addition, salinity and harvest time affected the oxalic acid content which was the lowest for the S2 treatment at the second harvest. Similarly, the richest fatty acid (α-linolenic acid) increased with increasing salinity at the first harvest. Salinity and harvest time also affected the antimicrobial properties, especially against Staphylococcus aureus, Bacillus cereus and Trichoderma viride, where the extracts from the S1 and S2 treatments showed high effectiveness. In contrast, the highest amounts of flavanones (pinocembrin derivatives) were detected in the control treatment (second harvest), which was also reflected to the highest antioxidant activity (TBARS) for the same treatment. In conclusion, C. raphanina subsp. mixta plants seem to be tolerant to medium salinity stress (S1 treatment) since plant growth was not severely impaired, while salinity and harvesting time affected the nutritional value (fat, proteins, and carbohydrates) and the chemical composition (tocopherols, sugars, oxalic acid, fatty acids), as well as the bioactive properties (cytotoxicity and antimicrobial properties) of the final product.
PB  - MDPI AG
T2  - Molecules
T1  - Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions
IS  - 9
VL  - 25
DO  - 10.3390/molecules25092204
SP  - 2204
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Ines and Pereira, Carla and Calhelha, Ricardo C. and Chrysargyris, Antonios and Tzortzakis, Nikolaos and Ivanov, Marija and Soković, Marina and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "The aim of this report was to study the effect of salinity (control: 2dS/m, S1: 4 dS/m and S2: 6 dS/m) and harvest time (first harvest on 9 May 2018 and second harvest on 19 April 2018) on the growth and the chemical composition of Centaurea raphanina subsp. mixta plants. The plants of the first harvest were used for the plant growth measurements (fresh weight and moisture content of leaves, rosette diameter, number and thickness of leaves), whereas those of the second harvest were not used for these measurements due to the flowering initiation, which made the leaves unmarketable due to their hard texture. The results of our study showed that C. raphanina subsp. mixta plants can be cultivated under mild salinity (S1 treatment) conditions without severe effects on plant growth and yield, since a more severe loss (27.5%) was observed for the S2 treatment. In addition, harvest time proved to be a cost-effective cultivation practice that allows to regulate the quality of the final product, either in edible form (first harvest) or for nutraceutical and pharmaceutical purposes as well as antimicrobial agents in food products. Therefore, the combination of these two agronomic factors showed interesting results in terms of the quality of the final product. In particular, high salinity (S2 treatment) improved the nutritional value by increasing the fat, proteins and carbohydrates contents in the first harvest, as well as the tocopherols and sugars contents (S1 and S2 treatments, respectively) in the second harvest. In addition, salinity and harvest time affected the oxalic acid content which was the lowest for the S2 treatment at the second harvest. Similarly, the richest fatty acid (α-linolenic acid) increased with increasing salinity at the first harvest. Salinity and harvest time also affected the antimicrobial properties, especially against Staphylococcus aureus, Bacillus cereus and Trichoderma viride, where the extracts from the S1 and S2 treatments showed high effectiveness. In contrast, the highest amounts of flavanones (pinocembrin derivatives) were detected in the control treatment (second harvest), which was also reflected to the highest antioxidant activity (TBARS) for the same treatment. In conclusion, C. raphanina subsp. mixta plants seem to be tolerant to medium salinity stress (S1 treatment) since plant growth was not severely impaired, while salinity and harvesting time affected the nutritional value (fat, proteins, and carbohydrates) and the chemical composition (tocopherols, sugars, oxalic acid, fatty acids), as well as the bioactive properties (cytotoxicity and antimicrobial properties) of the final product.",
publisher = "MDPI AG",
journal = "Molecules",
title = "Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions",
number = "9",
volume = "25",
doi = "10.3390/molecules25092204",
pages = "2204"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Chrysargyris, A., Tzortzakis, N., Ivanov, M., Soković, M., Barros, L.,& Ferreira, I. C. F. R.. (2020). Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions. in Molecules
MDPI AG., 25(9), 2204.
https://doi.org/10.3390/molecules25092204

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Chrysargyris A, Tzortzakis N, Ivanov M, Soković M, Barros L, Ferreira ICFR. Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions. in Molecules. 2020;25(9):2204.
doi:10.3390/molecules25092204 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Ines, Pereira, Carla, Calhelha, Ricardo C., Chrysargyris, Antonios, Tzortzakis, Nikolaos, Ivanov, Marija, Soković, Marina, Barros, Lillian, Ferreira, Isabel C. F. R., "Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions" in Molecules, 25, no. 9 (2020):2204,
https://doi.org/10.3390/molecules25092204 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Ines
AU  - Pereira, Carla
AU  - Calhelha, Ricardo
AU  - Di Gioia, Francesco
AU  - Tzortzakis, Nikolaos
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/4/314
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3664
AB  - Centaurea raphanina subsp. mixta (DC.) Runemark is a wild edible species endemic to Greece. This study evaluated the chemical composition and bioactive properties of wild and cultivated C. raphanina subsp. mixta plants. Wild plants had higher nutritional value than cultivated ones, whereas cultivated plants contained more tocopherols. Glucose and sucrose were higher in cultivated plants and trehalose in wild ones. Oxalic and total organic acids were detected in higher amounts in cultivated samples. The main fatty acids were α-linolenic, linoleic and palmitic acid, while wild plants were richer in polyunsaturated fatty acids. Two pinocembrin derivatives were the main phenolic compounds being detected in higher amounts in wild plants. Regarding the antioxidant activity, wild and cultivated plants were more effective in the oxidative haemolysis (OxHLIA) and thiobarbituric acid reactive substances (TBARS) assays, respectively. Moreover, both extracts showed moderate cytotoxicity in non-tumor cell lines (PLP2), while cultivated plants were more effective against cervical carcinoma (HeLa), breast carcinoma (MCF-7) and non-small lung cancer (NCI-H460) cell lines. Finally, wild plants showed higher antimicrobial activity than cultivated plants against specific pathogens. In conclusion, the cultivation of C. raphanina subsp. mixta showed promising results in terms of tocopherols content and antiproliferative effects, however further research is needed to decrease oxalic acid content.
PB  - MDPI AG
T2  - Antioxidants
T1  - Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds
IS  - 4
VL  - 9
DO  - 10.3390/antiox9040314
SP  - 314
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Ines and Pereira, Carla and Calhelha, Ricardo and Di Gioia, Francesco and Tzortzakis, Nikolaos and Ivanov, Marija and Soković, Marina and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "Centaurea raphanina subsp. mixta (DC.) Runemark is a wild edible species endemic to Greece. This study evaluated the chemical composition and bioactive properties of wild and cultivated C. raphanina subsp. mixta plants. Wild plants had higher nutritional value than cultivated ones, whereas cultivated plants contained more tocopherols. Glucose and sucrose were higher in cultivated plants and trehalose in wild ones. Oxalic and total organic acids were detected in higher amounts in cultivated samples. The main fatty acids were α-linolenic, linoleic and palmitic acid, while wild plants were richer in polyunsaturated fatty acids. Two pinocembrin derivatives were the main phenolic compounds being detected in higher amounts in wild plants. Regarding the antioxidant activity, wild and cultivated plants were more effective in the oxidative haemolysis (OxHLIA) and thiobarbituric acid reactive substances (TBARS) assays, respectively. Moreover, both extracts showed moderate cytotoxicity in non-tumor cell lines (PLP2), while cultivated plants were more effective against cervical carcinoma (HeLa), breast carcinoma (MCF-7) and non-small lung cancer (NCI-H460) cell lines. Finally, wild plants showed higher antimicrobial activity than cultivated plants against specific pathogens. In conclusion, the cultivation of C. raphanina subsp. mixta showed promising results in terms of tocopherols content and antiproliferative effects, however further research is needed to decrease oxalic acid content.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds",
number = "4",
volume = "9",
doi = "10.3390/antiox9040314",
pages = "314"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R., Di Gioia, F., Tzortzakis, N., Ivanov, M., Soković, M., Barros, L.,& Ferreira, I. C. F. R.. (2020). Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds. in Antioxidants
MDPI AG., 9(4), 314.
https://doi.org/10.3390/antiox9040314

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha R, Di Gioia F, Tzortzakis N, Ivanov M, Soković M, Barros L, Ferreira ICFR. Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds. in Antioxidants. 2020;9(4):314.
doi:10.3390/antiox9040314 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Ines, Pereira, Carla, Calhelha, Ricardo, Di Gioia, Francesco, Tzortzakis, Nikolaos, Ivanov, Marija, Soković, Marina, Barros, Lillian, Ferreira, Isabel C. F. R., "Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds" in Antioxidants, 9, no. 4 (2020):314,
https://doi.org/10.3390/antiox9040314 . .

TY  - JOUR
AU  - Caleja, Cristina
AU  - Barros, Lillian
AU  - Barreira, João C. M.
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Bento, Albino
AU  - Oliveira, M. Beatriz P. P.
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32096517
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3643
AB  - Replacing artificial additives by natural compounds is a current trend in the food industry. In addition to their preserving effect, naturally obtained ingredients often exhibit important levels of bioactivities. Generally, plant species represent better sources of natural ingredients, since their compounds are less prone to causing unpleasant taste or odour. Chestnut male flower (CMF) was reported to have high antioxidant and antimicrobial activities. Hence, it was tested as an alternative to potassium sorbate in the most treasured Portuguese delicacy: "pastel de nata". Different nutritional, chemical, physical and bioactive parameters were compared in two different periods: baking day and two days after baking. All samples presented similar nutritional and chemical profiles, but those added with CMF revealed higher contents of reducing agents and radical scavengers. Accordingly, the newly obtained formulation is expected to have better effects on consumers' health, maintaining the chemical characteristics, besides rendering a novel, economically profitable, application to CMF.
T2  - Food & Function
T1  - Castanea sativa male flower extracts as an alternative additive in the Portuguese pastry delicacy "pastel de nata".
IS  - 3
VL  - 11
DO  - 10.1039/c9fo03050f
SP  - 2208
EP  - 2217
ER  - 

@article{
author = "Caleja, Cristina and Barros, Lillian and Barreira, João C. M. and Soković, Marina and Calhelha, Ricardo C. and Bento, Albino and Oliveira, M. Beatriz P. P. and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "Replacing artificial additives by natural compounds is a current trend in the food industry. In addition to their preserving effect, naturally obtained ingredients often exhibit important levels of bioactivities. Generally, plant species represent better sources of natural ingredients, since their compounds are less prone to causing unpleasant taste or odour. Chestnut male flower (CMF) was reported to have high antioxidant and antimicrobial activities. Hence, it was tested as an alternative to potassium sorbate in the most treasured Portuguese delicacy: "pastel de nata". Different nutritional, chemical, physical and bioactive parameters were compared in two different periods: baking day and two days after baking. All samples presented similar nutritional and chemical profiles, but those added with CMF revealed higher contents of reducing agents and radical scavengers. Accordingly, the newly obtained formulation is expected to have better effects on consumers' health, maintaining the chemical characteristics, besides rendering a novel, economically profitable, application to CMF.",
journal = "Food & Function",
title = "Castanea sativa male flower extracts as an alternative additive in the Portuguese pastry delicacy "pastel de nata".",
number = "3",
volume = "11",
doi = "10.1039/c9fo03050f",
pages = "2208-2217"
}

Caleja, C., Barros, L., Barreira, J. C. M., Soković, M., Calhelha, R. C., Bento, A., Oliveira, M. B. P. P.,& Ferreira, I. C. F. R.. (2020). Castanea sativa male flower extracts as an alternative additive in the Portuguese pastry delicacy "pastel de nata".. in Food & Function, 11(3), 2208-2217.
https://doi.org/10.1039/c9fo03050f

Caleja C, Barros L, Barreira JCM, Soković M, Calhelha RC, Bento A, Oliveira MBPP, Ferreira ICFR. Castanea sativa male flower extracts as an alternative additive in the Portuguese pastry delicacy "pastel de nata".. in Food & Function. 2020;11(3):2208-2217.
doi:10.1039/c9fo03050f .

Caleja, Cristina, Barros, Lillian, Barreira, João C. M., Soković, Marina, Calhelha, Ricardo C., Bento, Albino, Oliveira, M. Beatriz P. P., Ferreira, Isabel C. F. R., "Castanea sativa male flower extracts as an alternative additive in the Portuguese pastry delicacy "pastel de nata"." in Food & Function, 11, no. 3 (2020):2208-2217,
https://doi.org/10.1039/c9fo03050f . .