TY  - CONF
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Lukić, Tamara
AU  - Gašić, Uroš
AU  - Petrović, Luka
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Božunović, Jelena
AU  - Filipović, Biljana
AU  - Todorović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - https://europlantbiology2023.org/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6481
AB  - Nepeta L. is the largest genus of the Lamiaceae family. The species of this genus are famous
for their biopesticidal potential and medicinal properties. Within the genus, we found
considerable variability in the qualitative and quantitative content of iridoid and phenolic
compounds. Using state-of-the-art metabolomics techniques (UHPLC/LTQ-Orbitrap MS,
GC/MS, UHPLC/DAD/(+/-)HESI-MS2), we comprehensively assessed the overall diversity of iridoids and phenolics in the selected chemodiverse Nepeta taxa at the intra-species level.
To reconstruct phylogenetic relations among the selected chemodiverse taxa from across the
Nepeta genus, genomic ITS molecular marker and two maternally inherited plastid DNA
regions (trnL-trnF and rbcL) were analyzed. A consensus phylogenetic tree was constructed
to interpret the distribution of the most abundant iridoid and phenolic compounds. It was
not possible to uncover clear regularities between phylogenetic relationship and chemotype
affiliation.
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta
SP  - 78
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6481
ER  - 

@conference{
author = "Banjanac, Tijana and Šiler, Branislav and Lukić, Tamara and Gašić, Uroš and Petrović, Luka and Matekalo, Dragana and Skorić, Marijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Milutinović, Milica and Aničić, Neda and Božunović, Jelena and Filipović, Biljana and Todorović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "Nepeta L. is the largest genus of the Lamiaceae family. The species of this genus are famous
for their biopesticidal potential and medicinal properties. Within the genus, we found
considerable variability in the qualitative and quantitative content of iridoid and phenolic
compounds. Using state-of-the-art metabolomics techniques (UHPLC/LTQ-Orbitrap MS,
GC/MS, UHPLC/DAD/(+/-)HESI-MS2), we comprehensively assessed the overall diversity of iridoids and phenolics in the selected chemodiverse Nepeta taxa at the intra-species level.
To reconstruct phylogenetic relations among the selected chemodiverse taxa from across the
Nepeta genus, genomic ITS molecular marker and two maternally inherited plastid DNA
regions (trnL-trnF and rbcL) were analyzed. A consensus phylogenetic tree was constructed
to interpret the distribution of the most abundant iridoid and phenolic compounds. It was
not possible to uncover clear regularities between phylogenetic relationship and chemotype
affiliation.",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta",
pages = "78-79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6481"
}

Banjanac, T., Šiler, B., Lukić, T., Gašić, U., Petrović, L., Matekalo, D., Skorić, M., Nestorović Živković, J., Dmitrović, S., Milutinović, M., Aničić, N., Božunović, J., Filipović, B., Todorović, M.,& Mišić, D.. (2023). Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_6481

Banjanac T, Šiler B, Lukić T, Gašić U, Petrović L, Matekalo D, Skorić M, Nestorović Živković J, Dmitrović S, Milutinović M, Aničić N, Božunović J, Filipović B, Todorović M, Mišić D. Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_6481 .

Banjanac, Tijana, Šiler, Branislav, Lukić, Tamara, Gašić, Uroš, Petrović, Luka, Matekalo, Dragana, Skorić, Marijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Milutinović, Milica, Aničić, Neda, Božunović, Jelena, Filipović, Biljana, Todorović, Miloš, Mišić, Danijela, "Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):78-79,
https://hdl.handle.net/21.15107/rcub_ibiss_6481 .

TY  - GEN
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Šiler, Branislav
AU  - Dmitrović, Slavica
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Petrović, Luka
AU  - Lukić, Tamara
AU  - Todorović, Miloš
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6560
AB  - Introduction: Recent intensive research on the genus Nepeta has resulted in increasingly accumulated
information regarding the biosynthetic pathways and chemical evolution of iridoids; however, several in-
triguing aspects remain yet to be resolved. Our objective was to deeply investigate the molecular back-
ground of the diversity of iridoid compounds within the genus Nepeta, as well as regulative mechanisms
of their biosynthesis.
Methods: Leaves of greenhouse-grown plants were analysed for the composition of iridoid aglycones
(IAs) and iridoid glucosides (IGs) adopting non-targeted (UHPLC/LTQ Orbitrap MS n
, GC/MS) and targeted
(UHPLC/DAD/(±)HESI-MS 2 ) metabolomics approaches. Following RNA-Seq, transcriptomes of phylodi-
verse Nepeta taxa were searched for the presence/absence of iridoid-related biosynthetic genes. Co-ex-
pression patterns (qPCR) of biosynthetic genes and transcription factors were determined following
plants’ exposure to various environmental stresses.
Results: The comparison of metabolite composition among phylodiverse Nepeta taxa provided a sys-
tematic understanding of qualitative and quantitative composition of iridoids in leaves of the selected
Nepeta taxa. Mining of RNA-Seq data in search for iridoid biosynthesis-related genes pointed to signifi-
cant differences between taxa producing both IAs and IGs and those producing only IGs. Comparative
metabolomics and gene co-expression analysis provided new information about mechanisms of regu-
lation of iridoid biosynthesis.
Conclusion: Integration of data from several techniques analysed by different methods resulted in iden-
tifying key genes involved in the regulation of metabolic flux through the iridoid biosynthetic pathway
and offered an explanation why some Nepeta taxa produce only IGs, while others produce both IAs and
IGs. The obtained results pointed to new gene targets for improving iridoid production through biotech-
nology-based approaches.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae)
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6560
ER  - 

@misc{
author = "Mišić, Danijela and Gašić, Uroš and Šiler, Branislav and Dmitrović, Slavica and Skorić, Marijana and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Aničić, Neda and Petrović, Luka and Lukić, Tamara and Todorović, Miloš",
year = "2023",
abstract = "Introduction: Recent intensive research on the genus Nepeta has resulted in increasingly accumulated
information regarding the biosynthetic pathways and chemical evolution of iridoids; however, several in-
triguing aspects remain yet to be resolved. Our objective was to deeply investigate the molecular back-
ground of the diversity of iridoid compounds within the genus Nepeta, as well as regulative mechanisms
of their biosynthesis.
Methods: Leaves of greenhouse-grown plants were analysed for the composition of iridoid aglycones
(IAs) and iridoid glucosides (IGs) adopting non-targeted (UHPLC/LTQ Orbitrap MS n
, GC/MS) and targeted
(UHPLC/DAD/(±)HESI-MS 2 ) metabolomics approaches. Following RNA-Seq, transcriptomes of phylodi-
verse Nepeta taxa were searched for the presence/absence of iridoid-related biosynthetic genes. Co-ex-
pression patterns (qPCR) of biosynthetic genes and transcription factors were determined following
plants’ exposure to various environmental stresses.
Results: The comparison of metabolite composition among phylodiverse Nepeta taxa provided a sys-
tematic understanding of qualitative and quantitative composition of iridoids in leaves of the selected
Nepeta taxa. Mining of RNA-Seq data in search for iridoid biosynthesis-related genes pointed to signifi-
cant differences between taxa producing both IAs and IGs and those producing only IGs. Comparative
metabolomics and gene co-expression analysis provided new information about mechanisms of regu-
lation of iridoid biosynthesis.
Conclusion: Integration of data from several techniques analysed by different methods resulted in iden-
tifying key genes involved in the regulation of metabolic flux through the iridoid biosynthetic pathway
and offered an explanation why some Nepeta taxa produce only IGs, while others produce both IAs and
IGs. The obtained results pointed to new gene targets for improving iridoid production through biotech-
nology-based approaches.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae)",
pages = "133",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6560"
}

Mišić, D., Gašić, U., Šiler, B., Dmitrović, S., Skorić, M., Matekalo, D., Nestorović Živković, J., Banjanac, T., Filipović, B., Milutinović, M., Božunović, J., Aničić, N., Petrović, L., Lukić, T.,& Todorović, M.. (2023). Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae). in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade., 133.
https://hdl.handle.net/21.15107/rcub_ibiss_6560

Mišić D, Gašić U, Šiler B, Dmitrović S, Skorić M, Matekalo D, Nestorović Živković J, Banjanac T, Filipović B, Milutinović M, Božunović J, Aničić N, Petrović L, Lukić T, Todorović M. Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae). in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;:133.
https://hdl.handle.net/21.15107/rcub_ibiss_6560 .

Mišić, Danijela, Gašić, Uroš, Šiler, Branislav, Dmitrović, Slavica, Skorić, Marijana, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Aničić, Neda, Petrović, Luka, Lukić, Tamara, Todorović, Miloš, "Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae)" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia (2023):133,
https://hdl.handle.net/21.15107/rcub_ibiss_6560 .

TY  - CONF
AU  - Mišić, Danijela
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Gašić, Uroš
AU  - Skorić, Marijana
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6083
AB  - Data on the distribution and diversity of iridoid glycosides (IGs) and iridoid aglycones (IAs) across the genus Nepeta (fam. Lamiaceae) are fragmentary; only rarely these two subgroups of iridoids are simultaneously analysed. Our ambition was to fulfil these knowledge gaps by adopting state-of-the-art metabolomics techniques (untargeted LC-HRMS and GC/MS) to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phyllo-diverse Nepeta taxa, and thus facilitate further reconstruction of the molecular background of this diversity. Analyzed taxa were robustly grouped into three major chemotypes: (A) producers of both IGs and IAs; (B) taxa exclusively producing IGs; and (C) iridoid-lacking taxa. Furthermore, Nepeta species belonging to the chemotype A displayed significant heterogeneity in their IAs composition, and were roughly classified into several sub-chemotypes based on the stereochemistry of the major nepetalactone. No clear regularities between chemotype affiliation and phylogenetic relatedness, reconstructed adopting DNA barcoding tools, was revealed. Transcriptomes of leaves of distinguished Nepeta chemotypes were mined for the transcripts of iridoid-related biosynthetic genes, to facilitate the assessment of the factors responsible for the presence/absence of iridoids, or specific subgroups of iridoids, in these plants. Integration of metabolomics and transcriptomics data pointed to several key genes in the biosynthetic pathway as significant determinants of the iridoid diversity within the genus: geraniol synthase (GES), iridoid synthase (ISY), NAD-dependent nepetalactol-related short-chain-dehydrogenases/reductases (NEPSs), and latex protein-like genes (MLPLs). This study provided a framework to revisit some of the iridoid-metabolism-related ecological and chemical evolution theories, which is the course of our further work.
PB  - Davis: University of California
C3  - Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
T1  - Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae)
SP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6083
ER  - 

@conference{
author = "Mišić, Danijela and Aničić, Neda and Matekalo, Dragana and Gašić, Uroš and Skorić, Marijana and Šiler, Branislav and Banjanac, Tijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Petrović, Luka and Todorović, Miloš and Lukić, Tamara",
year = "2023",
abstract = "Data on the distribution and diversity of iridoid glycosides (IGs) and iridoid aglycones (IAs) across the genus Nepeta (fam. Lamiaceae) are fragmentary; only rarely these two subgroups of iridoids are simultaneously analysed. Our ambition was to fulfil these knowledge gaps by adopting state-of-the-art metabolomics techniques (untargeted LC-HRMS and GC/MS) to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phyllo-diverse Nepeta taxa, and thus facilitate further reconstruction of the molecular background of this diversity. Analyzed taxa were robustly grouped into three major chemotypes: (A) producers of both IGs and IAs; (B) taxa exclusively producing IGs; and (C) iridoid-lacking taxa. Furthermore, Nepeta species belonging to the chemotype A displayed significant heterogeneity in their IAs composition, and were roughly classified into several sub-chemotypes based on the stereochemistry of the major nepetalactone. No clear regularities between chemotype affiliation and phylogenetic relatedness, reconstructed adopting DNA barcoding tools, was revealed. Transcriptomes of leaves of distinguished Nepeta chemotypes were mined for the transcripts of iridoid-related biosynthetic genes, to facilitate the assessment of the factors responsible for the presence/absence of iridoids, or specific subgroups of iridoids, in these plants. Integration of metabolomics and transcriptomics data pointed to several key genes in the biosynthetic pathway as significant determinants of the iridoid diversity within the genus: geraniol synthase (GES), iridoid synthase (ISY), NAD-dependent nepetalactol-related short-chain-dehydrogenases/reductases (NEPSs), and latex protein-like genes (MLPLs). This study provided a framework to revisit some of the iridoid-metabolism-related ecological and chemical evolution theories, which is the course of our further work.",
publisher = "Davis: University of California",
journal = "Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA",
title = "Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae)",
pages = "100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6083"
}

Mišić, D., Aničić, N., Matekalo, D., Gašić, U., Skorić, M., Šiler, B., Banjanac, T., Nestorović Živković, J., Dmitrović, S., Filipović, B., Milutinović, M., Božunović, J., Petrović, L., Todorović, M.,& Lukić, T.. (2023). Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae). in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
Davis: University of California., 100.
https://hdl.handle.net/21.15107/rcub_ibiss_6083

Mišić D, Aničić N, Matekalo D, Gašić U, Skorić M, Šiler B, Banjanac T, Nestorović Živković J, Dmitrović S, Filipović B, Milutinović M, Božunović J, Petrović L, Todorović M, Lukić T. Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae). in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. 2023;:100.
https://hdl.handle.net/21.15107/rcub_ibiss_6083 .

Mišić, Danijela, Aničić, Neda, Matekalo, Dragana, Gašić, Uroš, Skorić, Marijana, Šiler, Branislav, Banjanac, Tijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, "Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae)" in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA (2023):100,
https://hdl.handle.net/21.15107/rcub_ibiss_6083 .

TY  - CONF
AU  - Mišić, Danijela
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Gašić, Uroš
AU  - Skorić, Marijana
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6480
AB  - Aim: The chemical richness of iridoids in the genus Nepeta, the exclusive representative of the subfamily Nepetoidae (Lamiaceae) that produces iridoids, has complex roles in nature, facilitating interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavour additives, fragrances, as well as toys for cats, and their bioactivities are usually assigned to nepetalactones. Data on the distribution and diversity of iridoids across the genus Nepeta are quite fragmental and only rarely, the two subgroups, iridoid glycosides (IGs) and iridoid aglycones (IAs), are simultaneously analysed. Our aim was to develop and adopt non-targeted metabolomics methods to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phylodiverse Nepeta taxa, and thus facilitate reconstruction of this diversity at the molecular level.
Material and methods: Metabolomics data were acquired adopting UHPLC/Orbitrap MS, UHPLC/QToF MS, and GC/MS. Leaves of phylodiverse/chemodiverse Nepeta taxa were subjected to RNA-Seq. DNA barcoding analysis was performed using chloroplast markers (matK, rbcL, trnL-trnF) and nuclear ribosomal ITS marker.
Main results: Nepeta taxa were robustly grouped into three major chemotypes: (1) producers of both IGs and IAs; (2) taxa exclusively producing IGs; and (3) taxa lacking iridoids. Chemotype A clade was further roughly classified into several sub-chemotypes based on the stereochemistry of the major compound  nepetalactone. Phylogenetic relations between the analysed taxa were reconstructed adopting a selected set of DNA barcoding markers. Transcriptomes of leaves of the distinguished Nepeta chemotypes were searched for transcripts of iridoid-related biosynthetic genes to facilitate the assessment of factors responsible for the presence/absence of IAs and IGs in these plants.
Conclusion: Integration of metabolomics, transcriptomics and DNA barcoding data revealed no clear regularities between chemotype affiliation and phylogenetic relatedness in analysed Nepeta taxa and pointed to several key genes in the iridoid’s biosynthetic pathway as significant determinants of the their diversity within the genus.
PB  - Farrago
C3  - Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria
T1  - Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae)
SP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6480
ER  - 

@conference{
author = "Mišić, Danijela and Aničić, Neda and Matekalo, Dragana and Gašić, Uroš and Skorić, Marijana and Šiler, Branislav and Banjanac, Tijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Petrović, Luka and Todorović, Miloš and Lukić, Tamara",
year = "2023",
abstract = "Aim: The chemical richness of iridoids in the genus Nepeta, the exclusive representative of the subfamily Nepetoidae (Lamiaceae) that produces iridoids, has complex roles in nature, facilitating interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavour additives, fragrances, as well as toys for cats, and their bioactivities are usually assigned to nepetalactones. Data on the distribution and diversity of iridoids across the genus Nepeta are quite fragmental and only rarely, the two subgroups, iridoid glycosides (IGs) and iridoid aglycones (IAs), are simultaneously analysed. Our aim was to develop and adopt non-targeted metabolomics methods to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phylodiverse Nepeta taxa, and thus facilitate reconstruction of this diversity at the molecular level.
Material and methods: Metabolomics data were acquired adopting UHPLC/Orbitrap MS, UHPLC/QToF MS, and GC/MS. Leaves of phylodiverse/chemodiverse Nepeta taxa were subjected to RNA-Seq. DNA barcoding analysis was performed using chloroplast markers (matK, rbcL, trnL-trnF) and nuclear ribosomal ITS marker.
Main results: Nepeta taxa were robustly grouped into three major chemotypes: (1) producers of both IGs and IAs; (2) taxa exclusively producing IGs; and (3) taxa lacking iridoids. Chemotype A clade was further roughly classified into several sub-chemotypes based on the stereochemistry of the major compound  nepetalactone. Phylogenetic relations between the analysed taxa were reconstructed adopting a selected set of DNA barcoding markers. Transcriptomes of leaves of the distinguished Nepeta chemotypes were searched for transcripts of iridoid-related biosynthetic genes to facilitate the assessment of factors responsible for the presence/absence of IAs and IGs in these plants.
Conclusion: Integration of metabolomics, transcriptomics and DNA barcoding data revealed no clear regularities between chemotype affiliation and phylogenetic relatedness in analysed Nepeta taxa and pointed to several key genes in the iridoid’s biosynthetic pathway as significant determinants of the their diversity within the genus.",
publisher = "Farrago",
journal = "Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria",
title = "Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae)",
pages = "77",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6480"
}

Mišić, D., Aničić, N., Matekalo, D., Gašić, U., Skorić, M., Šiler, B., Banjanac, T., Nestorović Živković, J., Dmitrović, S., Filipović, B., Milutinović, M., Božunović, J., Petrović, L., Todorović, M.,& Lukić, T.. (2023). Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae). in Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria
Farrago., 77.
https://hdl.handle.net/21.15107/rcub_ibiss_6480

Mišić D, Aničić N, Matekalo D, Gašić U, Skorić M, Šiler B, Banjanac T, Nestorović Živković J, Dmitrović S, Filipović B, Milutinović M, Božunović J, Petrović L, Todorović M, Lukić T. Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae). in Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria. 2023;:77.
https://hdl.handle.net/21.15107/rcub_ibiss_6480 .

Mišić, Danijela, Aničić, Neda, Matekalo, Dragana, Gašić, Uroš, Skorić, Marijana, Šiler, Branislav, Banjanac, Tijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, "Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae)" in Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria (2023):77,
https://hdl.handle.net/21.15107/rcub_ibiss_6480 .

TY  - CONF
AU  - Šiler, Branislav
AU  - Petrović, Luka
AU  - Banjanac, Tijana
AU  - Lukić, Tamara
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Božunović, Jelena
AU  - Filipović, Biljana
AU  - Todorović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - https://europlantbiology2023.org/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6443
AB  - Numerous species belonging to the genus Nepeta are appreciated producers of various
phenolic and iridoid compounds that have tremendously vast applicative value in
pharmacology and agriculture but are also highly esteemed as chemotaxonomical markers.
Nepeta nuda is a widespread Eurasian species, found in forest edges and clearings. We have
collected leaf samples from 12 populations across the central Balkan Peninsula and
investigated their genetic and phytochemical variations. We used 3 genomic and 7 transcriptome-derived microsatellite markers and revealed relatively low variation within the
populations. Both qualitative metabolite profiling of methanol extracts (Q-TOFF LC/MS) and
essential oils (GC/MS) analysis pointed out to high inter- and intra-population variations,
especially regarding the content of iridoids. A UHPLC-QQQ-MS/MS approach targeted towards
quantification of the most abundant iridoids and phenolics was highly efficient in the
diversification of N. nuda populations.
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans
SP  - 186
EP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6443
ER  - 

@conference{
author = "Šiler, Branislav and Petrović, Luka and Banjanac, Tijana and Lukić, Tamara and Matekalo, Dragana and Skorić, Marijana and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Milutinović, Milica and Aničić, Neda and Božunović, Jelena and Filipović, Biljana and Todorović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "Numerous species belonging to the genus Nepeta are appreciated producers of various
phenolic and iridoid compounds that have tremendously vast applicative value in
pharmacology and agriculture but are also highly esteemed as chemotaxonomical markers.
Nepeta nuda is a widespread Eurasian species, found in forest edges and clearings. We have
collected leaf samples from 12 populations across the central Balkan Peninsula and
investigated their genetic and phytochemical variations. We used 3 genomic and 7 transcriptome-derived microsatellite markers and revealed relatively low variation within the
populations. Both qualitative metabolite profiling of methanol extracts (Q-TOFF LC/MS) and
essential oils (GC/MS) analysis pointed out to high inter- and intra-population variations,
especially regarding the content of iridoids. A UHPLC-QQQ-MS/MS approach targeted towards
quantification of the most abundant iridoids and phenolics was highly efficient in the
diversification of N. nuda populations.",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans",
pages = "186-187",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6443"
}

Šiler, B., Petrović, L., Banjanac, T., Lukić, T., Matekalo, D., Skorić, M., Gašić, U., Nestorović Živković, J., Dmitrović, S., Milutinović, M., Aničić, N., Božunović, J., Filipović, B., Todorović, M.,& Mišić, D.. (2023). Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 186-187.
https://hdl.handle.net/21.15107/rcub_ibiss_6443

Šiler B, Petrović L, Banjanac T, Lukić T, Matekalo D, Skorić M, Gašić U, Nestorović Živković J, Dmitrović S, Milutinović M, Aničić N, Božunović J, Filipović B, Todorović M, Mišić D. Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:186-187.
https://hdl.handle.net/21.15107/rcub_ibiss_6443 .

Šiler, Branislav, Petrović, Luka, Banjanac, Tijana, Lukić, Tamara, Matekalo, Dragana, Skorić, Marijana, Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Milutinović, Milica, Aničić, Neda, Božunović, Jelena, Filipović, Biljana, Todorović, Miloš, Mišić, Danijela, "Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):186-187,
https://hdl.handle.net/21.15107/rcub_ibiss_6443 .

TY  - GEN
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Dmitrović, Slavica
AU  - Petrović, Luka
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Anđelković, Boban
AU  - Dimitrijević, Milena
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6196
AB  - Nepeta L. holds a prominent position as the largest genus in the Lamiaceae family and serves as the exclusive representative of the iridoid-lacking Nepetoidae subfamily which produces iridoids. The genus showcases a rich chemodiversity, encompassing taxa both with and without iridoids. Our study investigates the genetic basis of iridoid diversity using omics-guided and functional genomics approaches. We identified functional iridoid synthases (ISY) in iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa L. (NnIS). Remarkably, N. nervosa carries a dormant iridoid biosynthetic platform, suggesting a loss of iridoid production during its evolutionary history. Moreover, we explore regulatory mechanisms through comparative iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs) under various stress conditions (e.g., dehydration, UV − B radiation, pathogens) or elicitors (MeJA). These mechanisms influence plant productivity and the presence/absence of iridoids or their specific groups (iridoid aglycones and iridoid glycosides). Our research offers valuable insights into the molecular mechanisms driving iridoid biosynthesis and the chemical evolution of iridoids within Nepeta. This study enhances our understanding of the intricate relationship between genetics and the environment, providing comprehensive insights into iridoid metabolism, chemical evolution, and ecology.
PB  - Belgrade: Faculty of Chemistry
T2  - Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
T1  - Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6196
ER  - 

@misc{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Božunović, Jelena and Nestorović Živković, Jasmina and Gašić, Uroš and Milutinović, Milica and Dmitrović, Slavica and Petrović, Luka and Filipović, Biljana and Banjanac, Tijana and Šiler, Branislav and Anđelković, Boban and Dimitrijević, Milena and Mišić, Danijela",
year = "2023",
abstract = "Nepeta L. holds a prominent position as the largest genus in the Lamiaceae family and serves as the exclusive representative of the iridoid-lacking Nepetoidae subfamily which produces iridoids. The genus showcases a rich chemodiversity, encompassing taxa both with and without iridoids. Our study investigates the genetic basis of iridoid diversity using omics-guided and functional genomics approaches. We identified functional iridoid synthases (ISY) in iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa L. (NnIS). Remarkably, N. nervosa carries a dormant iridoid biosynthetic platform, suggesting a loss of iridoid production during its evolutionary history. Moreover, we explore regulatory mechanisms through comparative iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs) under various stress conditions (e.g., dehydration, UV − B radiation, pathogens) or elicitors (MeJA). These mechanisms influence plant productivity and the presence/absence of iridoids or their specific groups (iridoid aglycones and iridoid glycosides). Our research offers valuable insights into the molecular mechanisms driving iridoid biosynthesis and the chemical evolution of iridoids within Nepeta. This study enhances our understanding of the intricate relationship between genetics and the environment, providing comprehensive insights into iridoid metabolism, chemical evolution, and ecology.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
title = "Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme",
pages = "156",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6196"
}

Aničić, N., Matekalo, D., Skorić, M., Božunović, J., Nestorović Živković, J., Gašić, U., Milutinović, M., Dmitrović, S., Petrović, L., Filipović, B., Banjanac, T., Šiler, B., Anđelković, B., Dimitrijević, M.,& Mišić, D.. (2023). Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
Belgrade: Faculty of Chemistry..
https://hdl.handle.net/21.15107/rcub_ibiss_6196

Aničić N, Matekalo D, Skorić M, Božunović J, Nestorović Živković J, Gašić U, Milutinović M, Dmitrović S, Petrović L, Filipović B, Banjanac T, Šiler B, Anđelković B, Dimitrijević M, Mišić D. Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. 2023;:null-156.
https://hdl.handle.net/21.15107/rcub_ibiss_6196 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Božunović, Jelena, Nestorović Živković, Jasmina, Gašić, Uroš, Milutinović, Milica, Dmitrović, Slavica, Petrović, Luka, Filipović, Biljana, Banjanac, Tijana, Šiler, Branislav, Anđelković, Boban, Dimitrijević, Milena, Mišić, Danijela, "Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme" in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6196 .

TY  - CONF
AU  - Matekalo, Dragana
AU  - Aničić, Neda
AU  - Gašić, Uroš
AU  - Skorić, Marijana
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6082
AB  - The genus Nepeta is characterized by a fascinating diversity of iridoid compounds. Some species within the genus are characterized by the presence of both iridoid glycosides (IGs) and iridoid aglycones (IAs), while others produce exclusively IGs, or completely lack iridoids. The absence of certain iridoids does not necessarily imply the absence of gene transcripts dedicated to the particular steps of their biosynthetic pathway. Studying the biosynthetic pathways of iridoids in chemodiverse Nepeta species, while taking into account tissue-specific profiles of metabolites and relevant genes expression, may give us further insight into the mechanisms of regulation of the biosynthesis of these compounds. In this work we performed a comparative transcriptomic analysis of glandular trichomes and abraded leaves of Nepeta rtanjensis, an IAs- and IGs –rich species, and of IGs-producing N. grandiflora. The results of this analysis provided us with very informative data that can be used for parallel study of iridoid-related biosynthetic genes (BGs) and transcription factors (TFs) of the two chemodiverse taxa. On the other hand, differentially expressed genes in the investigated tissues, enabled us to generate a list of candidate genes for yet unelucidated steps of the iridoid metabolic pathway. These results combined with state-of-the-art metabolomics data bring us closer to understanding the molecular background of iridoid diversity within the genus Nepeta, and point to the factors involved in the regulation of the two distinct branches of the iridoid biosynthetic pathway, leading to either IGs or IAs.
PB  - Davis: University of California
C3  - Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
T1  - Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6082
ER  - 

@conference{
author = "Matekalo, Dragana and Aničić, Neda and Gašić, Uroš and Skorić, Marijana and Šiler, Branislav and Banjanac, Tijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Petrović, Luka and Todorović, Miloš and Lukić, Tamara and Mišić, Danijela",
year = "2023",
abstract = "The genus Nepeta is characterized by a fascinating diversity of iridoid compounds. Some species within the genus are characterized by the presence of both iridoid glycosides (IGs) and iridoid aglycones (IAs), while others produce exclusively IGs, or completely lack iridoids. The absence of certain iridoids does not necessarily imply the absence of gene transcripts dedicated to the particular steps of their biosynthetic pathway. Studying the biosynthetic pathways of iridoids in chemodiverse Nepeta species, while taking into account tissue-specific profiles of metabolites and relevant genes expression, may give us further insight into the mechanisms of regulation of the biosynthesis of these compounds. In this work we performed a comparative transcriptomic analysis of glandular trichomes and abraded leaves of Nepeta rtanjensis, an IAs- and IGs –rich species, and of IGs-producing N. grandiflora. The results of this analysis provided us with very informative data that can be used for parallel study of iridoid-related biosynthetic genes (BGs) and transcription factors (TFs) of the two chemodiverse taxa. On the other hand, differentially expressed genes in the investigated tissues, enabled us to generate a list of candidate genes for yet unelucidated steps of the iridoid metabolic pathway. These results combined with state-of-the-art metabolomics data bring us closer to understanding the molecular background of iridoid diversity within the genus Nepeta, and point to the factors involved in the regulation of the two distinct branches of the iridoid biosynthetic pathway, leading to either IGs or IAs.",
publisher = "Davis: University of California",
journal = "Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA",
title = "Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6082"
}

Matekalo, D., Aničić, N., Gašić, U., Skorić, M., Šiler, B., Banjanac, T., Nestorović Živković, J., Dmitrović, S., Filipović, B., Milutinović, M., Božunović, J., Petrović, L., Todorović, M., Lukić, T.,& Mišić, D.. (2023). Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
Davis: University of California., 82.
https://hdl.handle.net/21.15107/rcub_ibiss_6082

Matekalo D, Aničić N, Gašić U, Skorić M, Šiler B, Banjanac T, Nestorović Živković J, Dmitrović S, Filipović B, Milutinović M, Božunović J, Petrović L, Todorović M, Lukić T, Mišić D. Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. 2023;:82.
https://hdl.handle.net/21.15107/rcub_ibiss_6082 .

Matekalo, Dragana, Aničić, Neda, Gašić, Uroš, Skorić, Marijana, Šiler, Branislav, Banjanac, Tijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, Mišić, Danijela, "Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data" in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA (2023):82,
https://hdl.handle.net/21.15107/rcub_ibiss_6082 .

TY  - CONF
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Gašić, Uroš
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6081
AB  - Within a large iridoids-lacking clade of Nepetoidae (Lamiaceae), the genus Nepeta stands out as an exclusive producer of these atypical monoterpenes with a unique stereochemistry. The genus Nepeta exhibits a high diversity of iridoids, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. Comparative metabolomics, transcriptomics, gene co-expression, and phylogenetic analysis were conducted on the trans,cis-NL-rich N. rtanjensis Diklić & Milojević, the cis,trans-NL-dominant N. sibirica L., and the iridoid-lacking N. nervosa Royle & Bentham, revealing significant structural and functional differences in their iridoid synthases (ISY). N. rtanjensis was found to have two orthologues of ISY (NrISY1 and NrISY2), while N. sibirica and N. nervosa each had only one ISY (NsISY and NnISY, respectively). Although the phylogenetic analysis grouped NrISY1, NsISY, and NnISY with non-active Family 1 isoforms, organ-specific expression highlighted NrISY2, NsISY, and NnISY as promising ISY candidates. In vitro assays confirmed their function and NrISY2 was the most efficient in converting 8-oxogeranial to cis,trans-nepetalactol. However, 3D modeling and molecular docking analyses suggested that NnISY may have a unique cyclase activity. Likely, under in vivo conditions, the NnISY lacks substrates to catalyze, as a result of the loss of upstream geraniol synthase (GES) activity, which results in the loss of iridoids in N. nervosa. Presumably, some of the downstream pathway genes (NEPSs and MLPLs), essential for the NL biosynthesis, also underwent structural and activity changes in this species. Our ongoing work is conducted towards testing these robust hypotheses and re-establishing iridoid biosynthesis in N. nervosa.
PB  - Davis: University of California
C3  - Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
T1  - Functional iridoid synthases from three Nepeta species with differing iridoid  profiles
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6081
ER  - 

@conference{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Gašić, Uroš and Dimitrijević, Milena and Anđelković, Boban and Božunović, Jelena and Milutinović, Milica and Petrović, Luka and Mišić, Danijela",
year = "2023",
abstract = "Within a large iridoids-lacking clade of Nepetoidae (Lamiaceae), the genus Nepeta stands out as an exclusive producer of these atypical monoterpenes with a unique stereochemistry. The genus Nepeta exhibits a high diversity of iridoids, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. Comparative metabolomics, transcriptomics, gene co-expression, and phylogenetic analysis were conducted on the trans,cis-NL-rich N. rtanjensis Diklić & Milojević, the cis,trans-NL-dominant N. sibirica L., and the iridoid-lacking N. nervosa Royle & Bentham, revealing significant structural and functional differences in their iridoid synthases (ISY). N. rtanjensis was found to have two orthologues of ISY (NrISY1 and NrISY2), while N. sibirica and N. nervosa each had only one ISY (NsISY and NnISY, respectively). Although the phylogenetic analysis grouped NrISY1, NsISY, and NnISY with non-active Family 1 isoforms, organ-specific expression highlighted NrISY2, NsISY, and NnISY as promising ISY candidates. In vitro assays confirmed their function and NrISY2 was the most efficient in converting 8-oxogeranial to cis,trans-nepetalactol. However, 3D modeling and molecular docking analyses suggested that NnISY may have a unique cyclase activity. Likely, under in vivo conditions, the NnISY lacks substrates to catalyze, as a result of the loss of upstream geraniol synthase (GES) activity, which results in the loss of iridoids in N. nervosa. Presumably, some of the downstream pathway genes (NEPSs and MLPLs), essential for the NL biosynthesis, also underwent structural and activity changes in this species. Our ongoing work is conducted towards testing these robust hypotheses and re-establishing iridoid biosynthesis in N. nervosa.",
publisher = "Davis: University of California",
journal = "Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA",
title = "Functional iridoid synthases from three Nepeta species with differing iridoid  profiles",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6081"
}

Aničić, N., Matekalo, D., Skorić, M., Nestorović Živković, J., Dmitrović, S., Gašić, U., Dimitrijević, M., Anđelković, B., Božunović, J., Milutinović, M., Petrović, L.,& Mišić, D.. (2023). Functional iridoid synthases from three Nepeta species with differing iridoid  profiles. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
Davis: University of California., 80.
https://hdl.handle.net/21.15107/rcub_ibiss_6081

Aničić N, Matekalo D, Skorić M, Nestorović Živković J, Dmitrović S, Gašić U, Dimitrijević M, Anđelković B, Božunović J, Milutinović M, Petrović L, Mišić D. Functional iridoid synthases from three Nepeta species with differing iridoid  profiles. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. 2023;:80.
https://hdl.handle.net/21.15107/rcub_ibiss_6081 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Gašić, Uroš, Dimitrijević, Milena, Anđelković, Boban, Božunović, Jelena, Milutinović, Milica, Petrović, Luka, Mišić, Danijela, "Functional iridoid synthases from three Nepeta species with differing iridoid  profiles" in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA (2023):80,
https://hdl.handle.net/21.15107/rcub_ibiss_6081 .

TY  - CONF
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Šiler, Branislav
AU  - Dmitrović, Slavica
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5561
AB  - The genus Nepeta, one of the largest genera of the Lamiaceae family (subfamily Nepetoidae, tribe Nepeteae) comprises around 250 herbaceous, perennial, rarely annual species, native to temperate Europe, Asia, North Africa, and the mountainous region of tropical Africa. Phytochemical investigations have revealed monoterpenes (iridoids), diterpenes, triterpenes, as well as phenolics, including flavonoids, to be the major specialized metabolites in these remarkable plants. The chemical richness of terpenoids and phenolics in catmints plays functional roles in nature, facilitating complex interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavor additives, fragrances, and toys for cats, and their extensively studied bioactivities are mainly assigned to iridoid monoterpenoids nepetalactones. These compounds are proven repellents against insects (e.g. mosquitos, cockroaches, ticks, flies, mites, termites), and promising bioherbicides against a vast array of weeds (e.g. amaranth, ragweed, goosefoot, dandelion).
In spite of the plenitude of fundamental research that has documented the vast bioactive potential of Nepeta species, their commercial exploitation is restrained. There are several important factors that make the commercialization of Nepeta sp. most challenging, including limited bioresources, limited availability of bioactive compounds in purified forms, challenges to optimize the cultivation and standardize the quality of plant material, low number of studies focusing on the effects on non-target organisms and on the mutual relationships of individual compounds, instability and relatively low retainability of volatile nepetalactones, and others.
The challenge ahead is to provide sustainable sources of bioactive compounds of Nepeta sp. and thus establish a background for their commercialization. One of the solutions might be the creation of alternative sources of these biomolecules through synthetic biology approaches, thereby developing microbial or plant systems producing bioactive compounds of interest. Essential prerequisites to achieve this is the comprehensive and simultaneous acquiring, analyzing and interpreting the overall diversity of specialized metabolites within the genus Nepeta, reconstruction of the molecular background of the chemical diversity and elucidation of the biosynthetic pathways, as well as adopting the biotechnology tools to scale-up the production of the compounds of interest.
PB  - Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences
C3  - Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria
T1  - Catmints (Nepeta sp.): Biological activities and applicative potential
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5561
ER  - 

@conference{
author = "Mišić, Danijela and Gašić, Uroš and Šiler, Branislav and Dmitrović, Slavica and Skorić, Marijana and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Aničić, Neda and Petrović, Luka and Todorović, Miloš and Lukić, Tamara",
year = "2023",
abstract = "The genus Nepeta, one of the largest genera of the Lamiaceae family (subfamily Nepetoidae, tribe Nepeteae) comprises around 250 herbaceous, perennial, rarely annual species, native to temperate Europe, Asia, North Africa, and the mountainous region of tropical Africa. Phytochemical investigations have revealed monoterpenes (iridoids), diterpenes, triterpenes, as well as phenolics, including flavonoids, to be the major specialized metabolites in these remarkable plants. The chemical richness of terpenoids and phenolics in catmints plays functional roles in nature, facilitating complex interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavor additives, fragrances, and toys for cats, and their extensively studied bioactivities are mainly assigned to iridoid monoterpenoids nepetalactones. These compounds are proven repellents against insects (e.g. mosquitos, cockroaches, ticks, flies, mites, termites), and promising bioherbicides against a vast array of weeds (e.g. amaranth, ragweed, goosefoot, dandelion).
In spite of the plenitude of fundamental research that has documented the vast bioactive potential of Nepeta species, their commercial exploitation is restrained. There are several important factors that make the commercialization of Nepeta sp. most challenging, including limited bioresources, limited availability of bioactive compounds in purified forms, challenges to optimize the cultivation and standardize the quality of plant material, low number of studies focusing on the effects on non-target organisms and on the mutual relationships of individual compounds, instability and relatively low retainability of volatile nepetalactones, and others.
The challenge ahead is to provide sustainable sources of bioactive compounds of Nepeta sp. and thus establish a background for their commercialization. One of the solutions might be the creation of alternative sources of these biomolecules through synthetic biology approaches, thereby developing microbial or plant systems producing bioactive compounds of interest. Essential prerequisites to achieve this is the comprehensive and simultaneous acquiring, analyzing and interpreting the overall diversity of specialized metabolites within the genus Nepeta, reconstruction of the molecular background of the chemical diversity and elucidation of the biosynthetic pathways, as well as adopting the biotechnology tools to scale-up the production of the compounds of interest.",
publisher = "Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences",
journal = "Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria",
title = "Catmints (Nepeta sp.): Biological activities and applicative potential",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5561"
}

Mišić, D., Gašić, U., Šiler, B., Dmitrović, S., Skorić, M., Matekalo, D., Nestorović Živković, J., Banjanac, T., Filipović, B., Milutinović, M., Božunović, J., Aničić, N., Petrović, L., Todorović, M.,& Lukić, T.. (2023). Catmints (Nepeta sp.): Biological activities and applicative potential. in Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria
Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_5561

Mišić D, Gašić U, Šiler B, Dmitrović S, Skorić M, Matekalo D, Nestorović Živković J, Banjanac T, Filipović B, Milutinović M, Božunović J, Aničić N, Petrović L, Todorović M, Lukić T. Catmints (Nepeta sp.): Biological activities and applicative potential. in Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria. 2023;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_5561 .

Mišić, Danijela, Gašić, Uroš, Šiler, Branislav, Dmitrović, Slavica, Skorić, Marijana, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Aničić, Neda, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, "Catmints (Nepeta sp.): Biological activities and applicative potential" in Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria (2023):18,
https://hdl.handle.net/21.15107/rcub_ibiss_5561 .

TY  - JOUR
AU  - Nestorović Živković, Jasmina
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Filipović, Biljana
AU  - Marković, Tijana
AU  - Dmitrović, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6561
AB  - Plants have developed a variety of defense mechanisms that allow them to recognize
and respond to specific stressors by triggering complex signaling networks that cause appropriate
biochemical changes to overcome the stress. In this study, we subjected in vitro grown N. nervosa
plants to PEG-induced dehydration stress for 1 day (1DPT), 3 days (3DPT) and 6 days (6DPT). Our
study investigated antioxidant enzyme activities, including catalase (CAT), peroxidase (POX) and
superoxide dismutase (SOD), unveiling dynamic responses to PEG-induced water stress. CAT levels
increased initially (1DPT) but declined with prolonged treatment; while POX activity significantly
increased at 3DPT and 6DPT; and SOD, particularly the Mn-SOD3 isoform, demonstrated a substantial
increase, emphasizing its role in the enzymatic free-radical scavenging activity. Furthermore,
examination of the phenolic acid content revealed that rosmarinic acid (RA) was the predominant
phenolic compound, followed by chlorogenic acid (CHLA), while ferulic acid (FA) and caffeic acid
(CAFFA) were present in lower concentrations. Notably, PEG-induced dehydration significantly
boosted RA content in N. nervosa plants at 3DPT. This increase highlights the plant’s response to
oxidative stress conditions and its role in non-enzymatic antioxidant defense mechanisms. These
findings significantly contribute to our comprehension of N. nervosa’s adaptive strategies under PEG induced dehydration stress, offering valuable insights into plant stress physiology within industrial
and agricultural contexts.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham.
IS  - 12
VL  - 9
DO  - 10.3390/horticulturae9121277
SP  - 1277
ER  - 

@article{
author = "Nestorović Živković, Jasmina and Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Filipović, Biljana and Marković, Tijana and Dmitrović, Slavica",
year = "2023",
abstract = "Plants have developed a variety of defense mechanisms that allow them to recognize
and respond to specific stressors by triggering complex signaling networks that cause appropriate
biochemical changes to overcome the stress. In this study, we subjected in vitro grown N. nervosa
plants to PEG-induced dehydration stress for 1 day (1DPT), 3 days (3DPT) and 6 days (6DPT). Our
study investigated antioxidant enzyme activities, including catalase (CAT), peroxidase (POX) and
superoxide dismutase (SOD), unveiling dynamic responses to PEG-induced water stress. CAT levels
increased initially (1DPT) but declined with prolonged treatment; while POX activity significantly
increased at 3DPT and 6DPT; and SOD, particularly the Mn-SOD3 isoform, demonstrated a substantial
increase, emphasizing its role in the enzymatic free-radical scavenging activity. Furthermore,
examination of the phenolic acid content revealed that rosmarinic acid (RA) was the predominant
phenolic compound, followed by chlorogenic acid (CHLA), while ferulic acid (FA) and caffeic acid
(CAFFA) were present in lower concentrations. Notably, PEG-induced dehydration significantly
boosted RA content in N. nervosa plants at 3DPT. This increase highlights the plant’s response to
oxidative stress conditions and its role in non-enzymatic antioxidant defense mechanisms. These
findings significantly contribute to our comprehension of N. nervosa’s adaptive strategies under PEG induced dehydration stress, offering valuable insights into plant stress physiology within industrial
and agricultural contexts.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham.",
number = "12",
volume = "9",
doi = "10.3390/horticulturae9121277",
pages = "1277"
}

Nestorović Živković, J., Aničić, N., Matekalo, D., Skorić, M., Filipović, B., Marković, T.,& Dmitrović, S.. (2023). Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham.. in Horticulturae
Basel: MDPI., 9(12), 1277.
https://doi.org/10.3390/horticulturae9121277

Nestorović Živković J, Aničić N, Matekalo D, Skorić M, Filipović B, Marković T, Dmitrović S. Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham.. in Horticulturae. 2023;9(12):1277.
doi:10.3390/horticulturae9121277 .

Nestorović Živković, Jasmina, Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Filipović, Biljana, Marković, Tijana, Dmitrović, Slavica, "Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham." in Horticulturae, 9, no. 12 (2023):1277,
https://doi.org/10.3390/horticulturae9121277 . .

TY  - JOUR
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6456
AB  - Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.
PB  - Lausanne, Switzerland: Frontiers
T2  - Frontiers in Plant Science
T1  - Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)
VL  - 14
DO  - 10.3389/fpls.2023.1211453
SP  - 1211453
ER  - 

@article{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Božunović, Jelena and Milutinović, Milica and Petrović, Luka and Dimitrijević, Milena and Anđelković, Boban and Mišić, Danijela",
year = "2023",
abstract = "Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.",
publisher = "Lausanne, Switzerland: Frontiers",
journal = "Frontiers in Plant Science",
title = "Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)",
volume = "14",
doi = "10.3389/fpls.2023.1211453",
pages = "1211453"
}

Aničić, N., Matekalo, D., Skorić, M., Gašić, U., Nestorović Živković, J., Dmitrović, S., Božunović, J., Milutinović, M., Petrović, L., Dimitrijević, M., Anđelković, B.,& Mišić, D.. (2023). Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae). in Frontiers in Plant Science
Lausanne, Switzerland: Frontiers., 14, 1211453.
https://doi.org/10.3389/fpls.2023.1211453

Aničić N, Matekalo D, Skorić M, Gašić U, Nestorović Živković J, Dmitrović S, Božunović J, Milutinović M, Petrović L, Dimitrijević M, Anđelković B, Mišić D. Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae). in Frontiers in Plant Science. 2023;14:1211453.
doi:10.3389/fpls.2023.1211453 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Božunović, Jelena, Milutinović, Milica, Petrović, Luka, Dimitrijević, Milena, Anđelković, Boban, Mišić, Danijela, "Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)" in Frontiers in Plant Science, 14 (2023):1211453,
https://doi.org/10.3389/fpls.2023.1211453 . .

TY  - JOUR
AU  - Gašić, Uroš
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Dmitrović, Slavica
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Živković, Suzana
AU  - Matekalo, Dragana
AU  - Filipović, Biljana
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2023.1155297/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5494
AB  - The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species ( D. lanata , D. ferruginea , and D. grandiflora ) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis ( Plantaginaceae ) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora . The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p -coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p -coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea . However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis .
T2  - Frontiers in Plant Science
T1  - Variation in the chemical profiles of three foxglove species in the central Balkans
VL  - 14
DO  - 10.3389/fpls.2023.1155297
ER  - 

@article{
author = "Gašić, Uroš and Banjanac, Tijana and Šiler, Branislav and Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Dmitrović, Slavica and Skorić, Marijana and Nestorović Živković, Jasmina and Petrović, Luka and Todorović, Miloš and Živković, Suzana and Matekalo, Dragana and Filipović, Biljana and Lukić, Tamara and Mišić, Danijela",
year = "2023",
abstract = "The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species ( D. lanata , D. ferruginea , and D. grandiflora ) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis ( Plantaginaceae ) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora . The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p -coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p -coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea . However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis .",
journal = "Frontiers in Plant Science",
title = "Variation in the chemical profiles of three foxglove species in the central Balkans",
volume = "14",
doi = "10.3389/fpls.2023.1155297"
}

Gašić, U., Banjanac, T., Šiler, B., Božunović, J., Milutinović, M., Aničić, N., Dmitrović, S., Skorić, M., Nestorović Živković, J., Petrović, L., Todorović, M., Živković, S., Matekalo, D., Filipović, B., Lukić, T.,& Mišić, D.. (2023). Variation in the chemical profiles of three foxglove species in the central Balkans. in Frontiers in Plant Science, 14.
https://doi.org/10.3389/fpls.2023.1155297

Gašić U, Banjanac T, Šiler B, Božunović J, Milutinović M, Aničić N, Dmitrović S, Skorić M, Nestorović Živković J, Petrović L, Todorović M, Živković S, Matekalo D, Filipović B, Lukić T, Mišić D. Variation in the chemical profiles of three foxglove species in the central Balkans. in Frontiers in Plant Science. 2023;14.
doi:10.3389/fpls.2023.1155297 .

Gašić, Uroš, Banjanac, Tijana, Šiler, Branislav, Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Dmitrović, Slavica, Skorić, Marijana, Nestorović Živković, Jasmina, Petrović, Luka, Todorović, Miloš, Živković, Suzana, Matekalo, Dragana, Filipović, Biljana, Lukić, Tamara, Mišić, Danijela, "Variation in the chemical profiles of three foxglove species in the central Balkans" in Frontiers in Plant Science, 14 (2023),
https://doi.org/10.3389/fpls.2023.1155297 . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Todorović, Miloš
AU  - Gašić, Uroš
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ivković, Đurđa
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Aničić, Neda
AU  - Banjanac, Tijana
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5505
AB  - The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening
IS  - 2
VL  - 12
DO  - 10.3390/antiox12020346
SP  - 346
ER  - 

@article{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Todorović, Miloš and Gašić, Uroš and Živković, Suzana and Skorić, Marijana and Ivković, Đurđa and Savić, Jelena and Devrnja, Nina and Aničić, Neda and Banjanac, Tijana and Mojović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening",
number = "2",
volume = "12",
doi = "10.3390/antiox12020346",
pages = "346"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Todorović, M., Gašić, U., Živković, S., Skorić, M., Ivković, Đ., Savić, J., Devrnja, N., Aničić, N., Banjanac, T., Mojović, M.,& Mišić, D.. (2023). Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants
Basel: MDPI., 12(2), 346.
https://doi.org/10.3390/antiox12020346

Milutinović M, Nakarada Đ, Božunović J, Todorović M, Gašić U, Živković S, Skorić M, Ivković Đ, Savić J, Devrnja N, Aničić N, Banjanac T, Mojović M, Mišić D. Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants. 2023;12(2):346.
doi:10.3390/antiox12020346 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Todorović, Miloš, Gašić, Uroš, Živković, Suzana, Skorić, Marijana, Ivković, Đurđa, Savić, Jelena, Devrnja, Nina, Aničić, Neda, Banjanac, Tijana, Mojović, Miloš, Mišić, Danijela, "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening" in Antioxidants, 12, no. 2 (2023):346,
https://doi.org/10.3390/antiox12020346 . .

TY  - CONF
AU  - Petrović, Luka
AU  - Skorić, Marijana
AU  - Aničić, Neda
AU  - Todorović, Miloš
AU  - Matekalo, Dragana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Lukić, Tamara
AU  - Filipović, Biljana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5568
AB  - Nepeta nuda L. (familija Lamiaceae) je, pored N. rtanjensis i N. cataria, jedna od tri vrste roda Nepeta koje rastu u Srbiji, naseljavajući prvenstveno sušna staništa na obodima šuma planinskih masiva. Poput većine predstavnika ovog roda, vrstu N. nuda karakteriše prisustvo različitih specijalizovanih metabolita, prvenstveno iridoida i fenolnih jedinjenja. Listovi jedinki N. nuda, prikupljeni su na različitim lokalitetima u istočnoj Srbiji: Izvor (Paraćin), Rtanj, Ozren i Barta Berilovac (Stara planina). Kvalitativni i kvantitativni hemijski sastav prikupljenog biljnog materijala analiziran je UHPLC/DAD/(±)HESI-MS2 metodom, usmerenom na dominantna bioaktivna jedinjenja iz grupa iridoida i polifenola. Rezultati metaboličkog profilisanja iridoida pokazali su da jedinke iz analiziranih populacija dominantno produkuju iridoidni glikozid - epideoksiloganinsku kiselinu, dok aglikoni iridoida nisu detektovani. Predstavnici svih ispitivanih populacija od fenolnih jedinjenja dominantno sintetišu ruzmarinsku kiselinu, dok su u tragovima detektovana jedinjenja kao što su kafeinska kiselina, izokvercitrin i astragalin. Sadržaj analiziranih jedinjenja varira između analiziranih populacija N. nuda. Populacija sa lokaliteta Balta Berilovac izdvaja se po visokom sadržaju epideoksiloganinske kiseline, što je čini interesantnom za dalja ispitivanja bioloških aktivnosti.
AB  - Nepeta nuda L. (фамилија Lamiaceae) је, поред N. rtanjensis и N. cataria, једна од три врсте рода Nepeta које расту у Србији, насељавајући првенствено сушна станишта на ободима шума планинских масива. Попут већине представника овог рода, врсту N. nuda карактерише присуство различитих специјализованих метаболита, првенствено иридоида и фенолних једињења. Листови јединки N. nuda, прикупљени су на различитим локалитетима у источној Србији: Извор (Параћин), Ртањ, Озрен и Барта Бериловац (Стара планина). Квалитативни и квантитативни хемијски састав прикупљеног биљног материјала анализиран је UHPLC/DAD/(±)HESI-MS2 методом, усмереном на доминантна биоактивна једињења из група иридоида и полифенола. Резултати метаболичког профилисања иридоида показали су да јединке из анализираних популација доминантно продукују иридоидни гликозид - епидеоксилоганинску киселину, док агликони иридоида нису детектовани. Представници свих испитиваних популација од фенолних једињења доминантно синтетишу рузмаринску киселину, док су у траговима детектована једињења као што су кафеинска киселина, изокверцитрин и астрагалин. Садржај анализираних једињења варира између анализираних популација N. nuda. Популација са локалитета Балта Бериловац издваја се по високом садржају епидеоксилоганинске киселине, што је чини интересантном за даља испитивања биолошких активности.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Хемијска карактеризација популација врсте Nepeta nuda L. из источне Србије
T1  - Hemijska karakterizacija populacija vrste Nepeta nuda L. iz istočne Srbije
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5568
ER  - 

@conference{
author = "Petrović, Luka and Skorić, Marijana and Aničić, Neda and Todorović, Miloš and Matekalo, Dragana and Milutinović, Milica and Božunović, Jelena and Nestorović Živković, Jasmina and Dmitrović, Slavica and Šiler, Branislav and Banjanac, Tijana and Lukić, Tamara and Filipović, Biljana and Gašić, Uroš and Mišić, Danijela",
year = "2022",
abstract = "Nepeta nuda L. (familija Lamiaceae) je, pored N. rtanjensis i N. cataria, jedna od tri vrste roda Nepeta koje rastu u Srbiji, naseljavajući prvenstveno sušna staništa na obodima šuma planinskih masiva. Poput većine predstavnika ovog roda, vrstu N. nuda karakteriše prisustvo različitih specijalizovanih metabolita, prvenstveno iridoida i fenolnih jedinjenja. Listovi jedinki N. nuda, prikupljeni su na različitim lokalitetima u istočnoj Srbiji: Izvor (Paraćin), Rtanj, Ozren i Barta Berilovac (Stara planina). Kvalitativni i kvantitativni hemijski sastav prikupljenog biljnog materijala analiziran je UHPLC/DAD/(±)HESI-MS2 metodom, usmerenom na dominantna bioaktivna jedinjenja iz grupa iridoida i polifenola. Rezultati metaboličkog profilisanja iridoida pokazali su da jedinke iz analiziranih populacija dominantno produkuju iridoidni glikozid - epideoksiloganinsku kiselinu, dok aglikoni iridoida nisu detektovani. Predstavnici svih ispitivanih populacija od fenolnih jedinjenja dominantno sintetišu ruzmarinsku kiselinu, dok su u tragovima detektovana jedinjenja kao što su kafeinska kiselina, izokvercitrin i astragalin. Sadržaj analiziranih jedinjenja varira između analiziranih populacija N. nuda. Populacija sa lokaliteta Balta Berilovac izdvaja se po visokom sadržaju epideoksiloganinske kiseline, što je čini interesantnom za dalja ispitivanja bioloških aktivnosti., Nepeta nuda L. (фамилија Lamiaceae) је, поред N. rtanjensis и N. cataria, једна од три врсте рода Nepeta које расту у Србији, насељавајући првенствено сушна станишта на ободима шума планинских масива. Попут већине представника овог рода, врсту N. nuda карактерише присуство различитих специјализованих метаболита, првенствено иридоида и фенолних једињења. Листови јединки N. nuda, прикупљени су на различитим локалитетима у источној Србији: Извор (Параћин), Ртањ, Озрен и Барта Бериловац (Стара планина). Квалитативни и квантитативни хемијски састав прикупљеног биљног материјала анализиран је UHPLC/DAD/(±)HESI-MS2 методом, усмереном на доминантна биоактивна једињења из група иридоида и полифенола. Резултати метаболичког профилисања иридоида показали су да јединке из анализираних популација доминантно продукују иридоидни гликозид - епидеоксилоганинску киселину, док агликони иридоида нису детектовани. Представници свих испитиваних популација од фенолних једињења доминантно синтетишу рузмаринску киселину, док су у траговима детектована једињења као што су кафеинска киселина, изокверцитрин и астрагалин. Садржај анализираних једињења варира између анализираних популација N. nuda. Популација са локалитета Балта Бериловац издваја се по високом садржају епидеоксилоганинске киселине, што је чини интересантном за даља испитивања биолошких активности.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Хемијска карактеризација популација врсте Nepeta nuda L. из источне Србије, Hemijska karakterizacija populacija vrste Nepeta nuda L. iz istočne Srbije",
pages = "61",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5568"
}

Petrović, L., Skorić, M., Aničić, N., Todorović, M., Matekalo, D., Milutinović, M., Božunović, J., Nestorović Živković, J., Dmitrović, S., Šiler, B., Banjanac, T., Lukić, T., Filipović, B., Gašić, U.,& Mišić, D.. (2022). Хемијска карактеризација популација врсте Nepeta nuda L. из источне Србије. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 61.
https://hdl.handle.net/21.15107/rcub_ibiss_5568

Petrović L, Skorić M, Aničić N, Todorović M, Matekalo D, Milutinović M, Božunović J, Nestorović Živković J, Dmitrović S, Šiler B, Banjanac T, Lukić T, Filipović B, Gašić U, Mišić D. Хемијска карактеризација популација врсте Nepeta nuda L. из источне Србије. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:61.
https://hdl.handle.net/21.15107/rcub_ibiss_5568 .

Petrović, Luka, Skorić, Marijana, Aničić, Neda, Todorović, Miloš, Matekalo, Dragana, Milutinović, Milica, Božunović, Jelena, Nestorović Živković, Jasmina, Dmitrović, Slavica, Šiler, Branislav, Banjanac, Tijana, Lukić, Tamara, Filipović, Biljana, Gašić, Uroš, Mišić, Danijela, "Хемијска карактеризација популација врсте Nepeta nuda L. из источне Србије" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):61,
https://hdl.handle.net/21.15107/rcub_ibiss_5568 .

TY  - CONF
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Aničić, Neda
AU  - Milutinović, Milica
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Šiler, Branislav
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Matekalo, Dragana
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Dragićević, Milan
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5564
AB  - The aim of this study was to determine qualitative composition of methanol-soluble metabolites in leaves
from in vitro-grown plants of phylogenetically distinct Nepeta species producing iridoids (N. sibirica L., N.
spicata Benth., N. argolica Bory & Chaub., and N. rtanjensis Diklić & Milojević) and iridoid-lacking N. nervosa
Royle ex Benth., adopting UHPLC-LTQ OrbiTrap MS technique. Totally, 52 compounds were identified
based on their monoisotopic masses, MSn fragmentation, and previously reported MS data and they were
clustered into four major groups: phenolic acid derivatives (23 compounds), flavonoids (10 compounds),
iridoids (13 compounds), and 6 compounds belonging to other classes. Both phenolic and iridoid profiles
of the tested species were found to be very diverse regarding both qualitative content of compounds and
their relative representation in extracts. Rosmarinic, 5-O-cafeoylquinic, and caffeic acids were found to be
the dominant phenolics in all analyzed species, especially in N. argolica, N. rtanjensis, and N. nervosa.
Iridoids were present in the form of glucosides and the most abundant compounds from this group were
epideoxyloganic acid, geniposide, and aucubin. Although iridoids were not detected in N. nervosa extracts,
this species was revealed as a rich source of phenolic acids and flavonoids. Conversely, N. spicata was found
to produce iridoids but negligible amounts of phenolic compounds. Extracts of two closely related endemic
species originating from the Balkan Peninsula, N. argolica and N. rtanjensis, were very similar in both
phenolic and iridoid profiles. The UHPLC-LTQ OrbiTrap MS method, in combination with chemometrics
data processing, was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic
determination and chemical differentiation of Nepeta species.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
T1  - Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5564
ER  - 

@conference{
author = "Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Aničić, Neda and Milutinović, Milica and Skorić, Marijana and Božunović, Jelena and Petrović, Luka and Šiler, Branislav and Filipović, Biljana and Banjanac, Tijana and Matekalo, Dragana and Todorović, Miloš and Lukić, Tamara and Dragićević, Milan and Mišić, Danijela",
year = "2022",
abstract = "The aim of this study was to determine qualitative composition of methanol-soluble metabolites in leaves
from in vitro-grown plants of phylogenetically distinct Nepeta species producing iridoids (N. sibirica L., N.
spicata Benth., N. argolica Bory & Chaub., and N. rtanjensis Diklić & Milojević) and iridoid-lacking N. nervosa
Royle ex Benth., adopting UHPLC-LTQ OrbiTrap MS technique. Totally, 52 compounds were identified
based on their monoisotopic masses, MSn fragmentation, and previously reported MS data and they were
clustered into four major groups: phenolic acid derivatives (23 compounds), flavonoids (10 compounds),
iridoids (13 compounds), and 6 compounds belonging to other classes. Both phenolic and iridoid profiles
of the tested species were found to be very diverse regarding both qualitative content of compounds and
their relative representation in extracts. Rosmarinic, 5-O-cafeoylquinic, and caffeic acids were found to be
the dominant phenolics in all analyzed species, especially in N. argolica, N. rtanjensis, and N. nervosa.
Iridoids were present in the form of glucosides and the most abundant compounds from this group were
epideoxyloganic acid, geniposide, and aucubin. Although iridoids were not detected in N. nervosa extracts,
this species was revealed as a rich source of phenolic acids and flavonoids. Conversely, N. spicata was found
to produce iridoids but negligible amounts of phenolic compounds. Extracts of two closely related endemic
species originating from the Balkan Peninsula, N. argolica and N. rtanjensis, were very similar in both
phenolic and iridoid profiles. The UHPLC-LTQ OrbiTrap MS method, in combination with chemometrics
data processing, was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic
determination and chemical differentiation of Nepeta species.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia",
title = "Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5564"
}

Gašić, U., Nestorović Živković, J., Dmitrović, S., Aničić, N., Milutinović, M., Skorić, M., Božunović, J., Petrović, L., Šiler, B., Filipović, B., Banjanac, T., Matekalo, D., Todorović, M., Lukić, T., Dragićević, M.,& Mišić, D.. (2022). Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
Ljubljana: Slovenian Society of Plant Biology., 35.
https://hdl.handle.net/21.15107/rcub_ibiss_5564

Gašić U, Nestorović Živković J, Dmitrović S, Aničić N, Milutinović M, Skorić M, Božunović J, Petrović L, Šiler B, Filipović B, Banjanac T, Matekalo D, Todorović M, Lukić T, Dragićević M, Mišić D. Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. 2022;:35.
https://hdl.handle.net/21.15107/rcub_ibiss_5564 .

Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Aničić, Neda, Milutinović, Milica, Skorić, Marijana, Božunović, Jelena, Petrović, Luka, Šiler, Branislav, Filipović, Biljana, Banjanac, Tijana, Matekalo, Dragana, Todorović, Miloš, Lukić, Tamara, Dragićević, Milan, Mišić, Danijela, "Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry" in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia (2022):35,
https://hdl.handle.net/21.15107/rcub_ibiss_5564 .

TY  - CONF
AU  - Skorić, Marijana
AU  - Aničić, Neda
AU  - Petrović, Luka
AU  - Gašić, Uroš
AU  - Matekalo, Dragana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Lukić, Tamara
AU  - Filipović, Biljana
AU  - Todorović, Miloš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5565
AB  - Род Nepeta (Lamiaceae) обухвата око 300 биљних врста које продукују различите специјализоване метаболите, при чему представља скоро једини род подфамилије Nepetoidae који производи иридоиде. Биљке рода Nepeta продукују иридоидне гликозиде и једноставне иридоиде – агликоне. Ова једињења се сматрају значајним за различите биолошке активности врста рода Nepeta, укључујући антимикробно, антитуморско, алелопатско и репелентно дејство. Извршена је детаљна фитохемијска анализа у циљу идентификације и квантификације иридоида одабраних представника различитих врста рода Nepeta: N. rtanjensis, N. laevigata, N. grandiflora, N. nervosa, N. parnassica, N. ernesti-mayeri, N. nuda, N. sibirica, N. stewartiana, N. nervosa. На основу добијених резултата извршено је раздвајање представника овог рода у неколико карактеристичних хемотипова, а на основу присуства иридоидних гликозида и/или иридоидних агликона. Спроведена су детаљна истраживања биосинтетског пута у оквиру рода Nepeta, са посебним акцентом на откривању ензима одговорних за реакције модификације иридоидног скелета (нпр. оксидације, редукције, гликозилације). Поред тога, описани су неки од механизама регулације биосинтезе иридоида током одговора биљака на абиотичке и биотичке факторе. Добијени резултати уз примену различитих биотехнолошких метода омогућиће таргетиране измене метаболизма у циљу повећане продукције иридоида од значаја.
AB  - Rod Nepeta (Lamiaceae) obuhvata oko 300 biljnih vrsta koje produkuju različite specijalizovane metabolite, pri čemu predstavlja skoro jedini rod podfamilije Nepetoidae koji proizvodi iridoide. Biljke roda Nepeta produkuju iridoidne glikozide i jednostavne iridoide – aglikone. Ova jedinjenja se smatraju značajnim za različite biološke aktivnosti vrsta roda Nepeta, uključujući antimikrobno, antitumorsko, alelopatsko i repelentno dejstvo. Izvršena je detaljna fitohemijska analiza u cilju identifikacije i kvantifikacije iridoida odabranih predstavnika različitih vrsta roda Nepeta: N. rtanjensis, N. laevigata, N. grandiflora, N. nervosa, N. parnassica, N. ernesti-mayeri, N. nuda, N. sibirica, N. stewartiana, N. nervosa. Na osnovu dobijenih rezultata izvršeno je razdvajanje predstavnika ovog roda u nekoliko karakterističnih hemotipova, a na osnovu prisustva iridoidnih glikozida i/ili iridoidnih aglikona. Sprovedena su detaljna istraživanja biosintetskog puta u okviru roda Nepeta, sa posebnim akcentom na otkrivanju enzima odgovornih za reakcije modifikacije iridoidnog skeleta (npr. oksidacije, redukcije, glikozilacije). Pored toga, opisani su neki od mehanizama regulacije biosinteze iridoida tokom odgovora biljaka na abiotičke i biotičke faktore. Dobijeni rezultati uz primenu različitih biotehnoloških metoda omogućiće targetirane izmene metabolizma u cilju povećane produkcije iridoida od značaja.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Proučavanje metabolizma iridoida u okviru roda Nepeta: značaj i perspektiva.
T1  - Проучавање метаболизма иридоида у оквиру рода Непета: значај и перспектива
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5565
ER  - 

@conference{
author = "Skorić, Marijana and Aničić, Neda and Petrović, Luka and Gašić, Uroš and Matekalo, Dragana and Milutinović, Milica and Božunović, Jelena and Nestorović Živković, Jasmina and Dmitrović, Slavica and Šiler, Branislav and Banjanac, Tijana and Lukić, Tamara and Filipović, Biljana and Todorović, Miloš and Mišić, Danijela",
year = "2022",
abstract = "Род Nepeta (Lamiaceae) обухвата око 300 биљних врста које продукују различите специјализоване метаболите, при чему представља скоро једини род подфамилије Nepetoidae који производи иридоиде. Биљке рода Nepeta продукују иридоидне гликозиде и једноставне иридоиде – агликоне. Ова једињења се сматрају значајним за различите биолошке активности врста рода Nepeta, укључујући антимикробно, антитуморско, алелопатско и репелентно дејство. Извршена је детаљна фитохемијска анализа у циљу идентификације и квантификације иридоида одабраних представника различитих врста рода Nepeta: N. rtanjensis, N. laevigata, N. grandiflora, N. nervosa, N. parnassica, N. ernesti-mayeri, N. nuda, N. sibirica, N. stewartiana, N. nervosa. На основу добијених резултата извршено је раздвајање представника овог рода у неколико карактеристичних хемотипова, а на основу присуства иридоидних гликозида и/или иридоидних агликона. Спроведена су детаљна истраживања биосинтетског пута у оквиру рода Nepeta, са посебним акцентом на откривању ензима одговорних за реакције модификације иридоидног скелета (нпр. оксидације, редукције, гликозилације). Поред тога, описани су неки од механизама регулације биосинтезе иридоида током одговора биљака на абиотичке и биотичке факторе. Добијени резултати уз примену различитих биотехнолошких метода омогућиће таргетиране измене метаболизма у циљу повећане продукције иридоида од значаја., Rod Nepeta (Lamiaceae) obuhvata oko 300 biljnih vrsta koje produkuju različite specijalizovane metabolite, pri čemu predstavlja skoro jedini rod podfamilije Nepetoidae koji proizvodi iridoide. Biljke roda Nepeta produkuju iridoidne glikozide i jednostavne iridoide – aglikone. Ova jedinjenja se smatraju značajnim za različite biološke aktivnosti vrsta roda Nepeta, uključujući antimikrobno, antitumorsko, alelopatsko i repelentno dejstvo. Izvršena je detaljna fitohemijska analiza u cilju identifikacije i kvantifikacije iridoida odabranih predstavnika različitih vrsta roda Nepeta: N. rtanjensis, N. laevigata, N. grandiflora, N. nervosa, N. parnassica, N. ernesti-mayeri, N. nuda, N. sibirica, N. stewartiana, N. nervosa. Na osnovu dobijenih rezultata izvršeno je razdvajanje predstavnika ovog roda u nekoliko karakterističnih hemotipova, a na osnovu prisustva iridoidnih glikozida i/ili iridoidnih aglikona. Sprovedena su detaljna istraživanja biosintetskog puta u okviru roda Nepeta, sa posebnim akcentom na otkrivanju enzima odgovornih za reakcije modifikacije iridoidnog skeleta (npr. oksidacije, redukcije, glikozilacije). Pored toga, opisani su neki od mehanizama regulacije biosinteze iridoida tokom odgovora biljaka na abiotičke i biotičke faktore. Dobijeni rezultati uz primenu različitih biotehnoloških metoda omogućiće targetirane izmene metabolizma u cilju povećane produkcije iridoida od značaja.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Proučavanje metabolizma iridoida u okviru roda Nepeta: značaj i perspektiva., Проучавање метаболизма иридоида у оквиру рода Непета: значај и перспектива",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5565"
}

Skorić, M., Aničić, N., Petrović, L., Gašić, U., Matekalo, D., Milutinović, M., Božunović, J., Nestorović Živković, J., Dmitrović, S., Šiler, B., Banjanac, T., Lukić, T., Filipović, B., Todorović, M.,& Mišić, D.. (2022). Proučavanje metabolizma iridoida u okviru roda Nepeta: značaj i perspektiva.. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 35.
https://hdl.handle.net/21.15107/rcub_ibiss_5565

Skorić M, Aničić N, Petrović L, Gašić U, Matekalo D, Milutinović M, Božunović J, Nestorović Živković J, Dmitrović S, Šiler B, Banjanac T, Lukić T, Filipović B, Todorović M, Mišić D. Proučavanje metabolizma iridoida u okviru roda Nepeta: značaj i perspektiva.. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:35.
https://hdl.handle.net/21.15107/rcub_ibiss_5565 .

Skorić, Marijana, Aničić, Neda, Petrović, Luka, Gašić, Uroš, Matekalo, Dragana, Milutinović, Milica, Božunović, Jelena, Nestorović Živković, Jasmina, Dmitrović, Slavica, Šiler, Branislav, Banjanac, Tijana, Lukić, Tamara, Filipović, Biljana, Todorović, Miloš, Mišić, Danijela, "Proučavanje metabolizma iridoida u okviru roda Nepeta: značaj i perspektiva." in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):35,
https://hdl.handle.net/21.15107/rcub_ibiss_5565 .

TY  - CONF
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Aničić, Neda
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Šiler, Branislav
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Matekalo, Dragana
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5563
AB  - Many Nepeta species (fam. Lamiace, subfam. Nepetoideae, tribe Nepetae) are well known for their
pharmacological properties and are widely used in folk medicine as diuretic, diaphoretic, anti-tussive, antispasmodic, anti-asthmatic, febrifuge and sedative agents, against gastrointestinal and respiratory
hyperactive disorders (Formisano et al., 2011). They also show biopesticide potential and display
behavioural effect on cats. Various biological activities of Nepeta species are closely related to their main
secondary metabolites, among which terpenes and phenolics predominate. Two herbaceous perennial
Nepeta species investigated within the present study are widely distributed in EuroAsia. N. cataria L. is
spreaded from Central Europe to the Iranian plateaus and in Central Asia, and N. nuda L. grows throughout
Europe and European part of Russia, the Crimea, in the Dnieper area and on Carpathians in Ukraine, as well
as in Siberia (Boikova and Grishkina, 2019).
Untargeted metabolomics approach adopting UHPLC/LTQ Orbitrap−MSn revealed iridoids and phenolic
acids as the major groups of compounds in methanol extracts of both N. cataria and N. nuda.
UHPLC/(±)HESI−MS2 profiling of major compounds from these groups of metabolites revealed that
rosmarinic acid was the major phenolic acid in both species, while from the group of iridoids, 1,5,9-
epideoxyloganic acid predominated. Methanol extract of N. cataria was rich in iridoid aglycone cis,transnepetalactone. Based on the bioautography assays, methanol extracts of N. cataria and N. nuda show
moderate α-amylase activity, and display significant antioxidative activity, which is primarily ascribed to
high content of rosmarinic acid.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
T1  - Chemical constituents and biological activities of two Nepeta species: N. cataria L. and N. nuda L.
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5563
ER  - 

@conference{
author = "Nestorović Živković, Jasmina and Dmitrović, Slavica and Aničić, Neda and Gašić, Uroš and Milutinović, Milica and Skorić, Marijana and Božunović, Jelena and Petrović, Luka and Šiler, Branislav and Filipović, Biljana and Banjanac, Tijana and Matekalo, Dragana and Todorović, Miloš and Lukić, Tamara and Mišić, Danijela",
year = "2022",
abstract = "Many Nepeta species (fam. Lamiace, subfam. Nepetoideae, tribe Nepetae) are well known for their
pharmacological properties and are widely used in folk medicine as diuretic, diaphoretic, anti-tussive, antispasmodic, anti-asthmatic, febrifuge and sedative agents, against gastrointestinal and respiratory
hyperactive disorders (Formisano et al., 2011). They also show biopesticide potential and display
behavioural effect on cats. Various biological activities of Nepeta species are closely related to their main
secondary metabolites, among which terpenes and phenolics predominate. Two herbaceous perennial
Nepeta species investigated within the present study are widely distributed in EuroAsia. N. cataria L. is
spreaded from Central Europe to the Iranian plateaus and in Central Asia, and N. nuda L. grows throughout
Europe and European part of Russia, the Crimea, in the Dnieper area and on Carpathians in Ukraine, as well
as in Siberia (Boikova and Grishkina, 2019).
Untargeted metabolomics approach adopting UHPLC/LTQ Orbitrap−MSn revealed iridoids and phenolic
acids as the major groups of compounds in methanol extracts of both N. cataria and N. nuda.
UHPLC/(±)HESI−MS2 profiling of major compounds from these groups of metabolites revealed that
rosmarinic acid was the major phenolic acid in both species, while from the group of iridoids, 1,5,9-
epideoxyloganic acid predominated. Methanol extract of N. cataria was rich in iridoid aglycone cis,transnepetalactone. Based on the bioautography assays, methanol extracts of N. cataria and N. nuda show
moderate α-amylase activity, and display significant antioxidative activity, which is primarily ascribed to
high content of rosmarinic acid.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia",
title = "Chemical constituents and biological activities of two Nepeta species: N. cataria L. and N. nuda L.",
pages = "61",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5563"
}

Nestorović Živković, J., Dmitrović, S., Aničić, N., Gašić, U., Milutinović, M., Skorić, M., Božunović, J., Petrović, L., Šiler, B., Filipović, B., Banjanac, T., Matekalo, D., Todorović, M., Lukić, T.,& Mišić, D.. (2022). Chemical constituents and biological activities of two Nepeta species: N. cataria L. and N. nuda L.. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
Ljubljana: Slovenian Society of Plant Biology., 61.
https://hdl.handle.net/21.15107/rcub_ibiss_5563

Nestorović Živković J, Dmitrović S, Aničić N, Gašić U, Milutinović M, Skorić M, Božunović J, Petrović L, Šiler B, Filipović B, Banjanac T, Matekalo D, Todorović M, Lukić T, Mišić D. Chemical constituents and biological activities of two Nepeta species: N. cataria L. and N. nuda L.. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. 2022;:61.
https://hdl.handle.net/21.15107/rcub_ibiss_5563 .

Nestorović Živković, Jasmina, Dmitrović, Slavica, Aničić, Neda, Gašić, Uroš, Milutinović, Milica, Skorić, Marijana, Božunović, Jelena, Petrović, Luka, Šiler, Branislav, Filipović, Biljana, Banjanac, Tijana, Matekalo, Dragana, Todorović, Miloš, Lukić, Tamara, Mišić, Danijela, "Chemical constituents and biological activities of two Nepeta species: N. cataria L. and N. nuda L." in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia (2022):61,
https://hdl.handle.net/21.15107/rcub_ibiss_5563 .

TY  - CONF
AU  - Todorović, Miloš
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Petrović, Luka
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Gašić, Uroš
AU  - Živković, Suzana
AU  - Lukić, Tamara
AU  - Dmitrović, Slavica
AU  - Matekalo, Dragana
AU  - Šiler, Branislav
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5280
AB  - Species of the genus Centaurium (family Gentianaceae), well-known medicinal plants, threatened in the nature, are a rich source of bioactive compounds that are of great interest for the pharmaceutical and biotech industry. While the phytochemical characterization was investigated in detail in roots and leaves, nobody has so far tackled the question about the specialized metabolites composition in the centaury flowers. In this work, using untargeted metabolomics approach, we examined phytochemical differences in flowers of two centaury species, Centaurium pulchellum (Sw.) Druce and Centaurium tenuiflorum (Hoffmanns. & Link) Fritsch, both displaying variability in flowers coloration, which grades from white to pink. UHPLC-Orbitrap MS characterization of methanol extracts in a negative ionization mode resulted in the detection of 82 compounds in total. The identified compounds represented six structurally distinct groups: phenolic acid aglycones and glycosides; iridoid glycosides and derivatives; flavonoid glycosides and aglycones; xantone glucosides and aglycones; hydorxycinnamic acid amides and other compounds. All compounds found were identified by exact mass search of their deprotonated molecule [M – H]–, MS2, MS3 and MS4 fragmentation behavior, as well as by comparison with the available literature. Flavonoid and xantone glycosides and aglycones are the main classes of metabolites identified, and their content was strongly associated with the coloration of the flowers. However, a clear insight into the molecular mechanism underlying the centaury flower coloration is still lacking. Herein, the ultimate
goal of our research is to comparatevely profile the changes in metabolome and transcriptome
between pink and white centaury flowers in order to pinpoint key compounds and genes controlling
the flower color formation.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Comparative metabolomics of two Centaurium species displaying variable flower coloration phenotypes
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5280
ER  - 

@conference{
author = "Todorović, Miloš and Milutinović, Milica and Božunović, Jelena and Aničić, Neda and Petrović, Luka and Skorić, Marijana and Nestorović Živković, Jasmina and Banjanac, Tijana and Gašić, Uroš and Živković, Suzana and Lukić, Tamara and Dmitrović, Slavica and Matekalo, Dragana and Šiler, Branislav and Mišić, Danijela",
year = "2022",
abstract = "Species of the genus Centaurium (family Gentianaceae), well-known medicinal plants, threatened in the nature, are a rich source of bioactive compounds that are of great interest for the pharmaceutical and biotech industry. While the phytochemical characterization was investigated in detail in roots and leaves, nobody has so far tackled the question about the specialized metabolites composition in the centaury flowers. In this work, using untargeted metabolomics approach, we examined phytochemical differences in flowers of two centaury species, Centaurium pulchellum (Sw.) Druce and Centaurium tenuiflorum (Hoffmanns. & Link) Fritsch, both displaying variability in flowers coloration, which grades from white to pink. UHPLC-Orbitrap MS characterization of methanol extracts in a negative ionization mode resulted in the detection of 82 compounds in total. The identified compounds represented six structurally distinct groups: phenolic acid aglycones and glycosides; iridoid glycosides and derivatives; flavonoid glycosides and aglycones; xantone glucosides and aglycones; hydorxycinnamic acid amides and other compounds. All compounds found were identified by exact mass search of their deprotonated molecule [M – H]–, MS2, MS3 and MS4 fragmentation behavior, as well as by comparison with the available literature. Flavonoid and xantone glycosides and aglycones are the main classes of metabolites identified, and their content was strongly associated with the coloration of the flowers. However, a clear insight into the molecular mechanism underlying the centaury flower coloration is still lacking. Herein, the ultimate
goal of our research is to comparatevely profile the changes in metabolome and transcriptome
between pink and white centaury flowers in order to pinpoint key compounds and genes controlling
the flower color formation.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Comparative metabolomics of two Centaurium species displaying variable flower coloration phenotypes",
pages = "145",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5280"
}

Todorović, M., Milutinović, M., Božunović, J., Aničić, N., Petrović, L., Skorić, M., Nestorović Živković, J., Banjanac, T., Gašić, U., Živković, S., Lukić, T., Dmitrović, S., Matekalo, D., Šiler, B.,& Mišić, D.. (2022). Comparative metabolomics of two Centaurium species displaying variable flower coloration phenotypes. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 145.
https://hdl.handle.net/21.15107/rcub_ibiss_5280

Todorović M, Milutinović M, Božunović J, Aničić N, Petrović L, Skorić M, Nestorović Živković J, Banjanac T, Gašić U, Živković S, Lukić T, Dmitrović S, Matekalo D, Šiler B, Mišić D. Comparative metabolomics of two Centaurium species displaying variable flower coloration phenotypes. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:145.
https://hdl.handle.net/21.15107/rcub_ibiss_5280 .

Todorović, Miloš, Milutinović, Milica, Božunović, Jelena, Aničić, Neda, Petrović, Luka, Skorić, Marijana, Nestorović Živković, Jasmina, Banjanac, Tijana, Gašić, Uroš, Živković, Suzana, Lukić, Tamara, Dmitrović, Slavica, Matekalo, Dragana, Šiler, Branislav, Mišić, Danijela, "Comparative metabolomics of two Centaurium species displaying variable flower coloration phenotypes" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):145,
https://hdl.handle.net/21.15107/rcub_ibiss_5280 .

TY  - CONF
AU  - Petrović, Luka
AU  - Skorić, Marijana
AU  - Aničić, Neda
AU  - Todorović, Miloš
AU  - Matekalo, Dragana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Živković, Suzana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Lukić, Tamara
AU  - Filipović, Biljana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5279
AB  - Nepeta nuda L. (family Lamiaceae) is a widespread species characterized by the prevalence of bioactive specialized metabolites from the groups of iridoids and phenolic. Analyzed plants were grown in vitro and in greenhouse from seeds collected from eastern Serbia (“Debeli Lug”), obtained from other research groups (“Rhodopes”, Bulgaria), or commercially purchased (“Jellito”, Germany). The chemical composition of N. nuda leaves was analyzed using the UHPLC/DAD/(±)HESI±МS2 method, targeted towards dominant iridoid compounds. Both in vitro and greenhouse grown plants are rich in iridoid glycoside 1,5,9-epideoxyloganic acid (1,5,9– eDLA), and among analysed accessons N. nuda “Debeli Lug” was pointed out as the most productive one. When it comes to iridoid aglycones, it is noticeable that N. nuda “Jelitto” biosynthetizes cis, trans- isomer of nepetalactone (NL) while N. nuda “Rhodopes” and N. nuda “Debeli Lug” possess cis,cis-NL. Relative expression of early biosynthetic genes- EBGs (NnGPPS, NnGES, NnG80, Nn8HGO), in plants grown in vitro, is much higher in N. nuda “Debeli Lug“ accession, compared to N. nuda “Rhodopes” and N.nuda “Jellito”. In greenhouse grown plants, there is no particular regularity when it comes to the expression of EBGs in three N. nuda accessions. NEPS enzymes seem to be one of the key factors determining the metabolic flux and productivity of N. nuda. While plants grown in vitro and in greenhouse both express NnNEPS2, only plants grown in vitro express NnNEPS1.2. It is also noticeable that N. nuda “Debeli Lug“ accession displays the highest NnNEPS2 expression, and the lowest NnNEPS1.2 expression.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Iridoid profiles and expression patterns of iridoid-related biosynthetic genes in three Nepeta nuda L. accessions
SP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5279
ER  - 

@conference{
author = "Petrović, Luka and Skorić, Marijana and Aničić, Neda and Todorović, Miloš and Matekalo, Dragana and Milutinović, Milica and Božunović, Jelena and Živković, Suzana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Šiler, Branislav and Banjanac, Tijana and Lukić, Tamara and Filipović, Biljana and Gašić, Uroš and Mišić, Danijela",
year = "2022",
abstract = "Nepeta nuda L. (family Lamiaceae) is a widespread species characterized by the prevalence of bioactive specialized metabolites from the groups of iridoids and phenolic. Analyzed plants were grown in vitro and in greenhouse from seeds collected from eastern Serbia (“Debeli Lug”), obtained from other research groups (“Rhodopes”, Bulgaria), or commercially purchased (“Jellito”, Germany). The chemical composition of N. nuda leaves was analyzed using the UHPLC/DAD/(±)HESI±МS2 method, targeted towards dominant iridoid compounds. Both in vitro and greenhouse grown plants are rich in iridoid glycoside 1,5,9-epideoxyloganic acid (1,5,9– eDLA), and among analysed accessons N. nuda “Debeli Lug” was pointed out as the most productive one. When it comes to iridoid aglycones, it is noticeable that N. nuda “Jelitto” biosynthetizes cis, trans- isomer of nepetalactone (NL) while N. nuda “Rhodopes” and N. nuda “Debeli Lug” possess cis,cis-NL. Relative expression of early biosynthetic genes- EBGs (NnGPPS, NnGES, NnG80, Nn8HGO), in plants grown in vitro, is much higher in N. nuda “Debeli Lug“ accession, compared to N. nuda “Rhodopes” and N.nuda “Jellito”. In greenhouse grown plants, there is no particular regularity when it comes to the expression of EBGs in three N. nuda accessions. NEPS enzymes seem to be one of the key factors determining the metabolic flux and productivity of N. nuda. While plants grown in vitro and in greenhouse both express NnNEPS2, only plants grown in vitro express NnNEPS1.2. It is also noticeable that N. nuda “Debeli Lug“ accession displays the highest NnNEPS2 expression, and the lowest NnNEPS1.2 expression.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Iridoid profiles and expression patterns of iridoid-related biosynthetic genes in three Nepeta nuda L. accessions",
pages = "144",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5279"
}

Petrović, L., Skorić, M., Aničić, N., Todorović, M., Matekalo, D., Milutinović, M., Božunović, J., Živković, S., Nestorović Živković, J., Dmitrović, S., Šiler, B., Banjanac, T., Lukić, T., Filipović, B., Gašić, U.,& Mišić, D.. (2022). Iridoid profiles and expression patterns of iridoid-related biosynthetic genes in three Nepeta nuda L. accessions. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 144.
https://hdl.handle.net/21.15107/rcub_ibiss_5279

Petrović L, Skorić M, Aničić N, Todorović M, Matekalo D, Milutinović M, Božunović J, Živković S, Nestorović Živković J, Dmitrović S, Šiler B, Banjanac T, Lukić T, Filipović B, Gašić U, Mišić D. Iridoid profiles and expression patterns of iridoid-related biosynthetic genes in three Nepeta nuda L. accessions. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:144.
https://hdl.handle.net/21.15107/rcub_ibiss_5279 .

Petrović, Luka, Skorić, Marijana, Aničić, Neda, Todorović, Miloš, Matekalo, Dragana, Milutinović, Milica, Božunović, Jelena, Živković, Suzana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Šiler, Branislav, Banjanac, Tijana, Lukić, Tamara, Filipović, Biljana, Gašić, Uroš, Mišić, Danijela, "Iridoid profiles and expression patterns of iridoid-related biosynthetic genes in three Nepeta nuda L. accessions" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):144,
https://hdl.handle.net/21.15107/rcub_ibiss_5279 .

TY  - CONF
AU  - Dmitrović, Slavica
AU  - Nestorović Živković, Jasmina
AU  - Aničić, Neda
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Matekalo, Dragana
AU  - Petrović, Luka
AU  - Božunović, Jelena
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5570
AB  - Drough-stress conditions lead to excessive production of reactive oxygen species (ROS) and
oxidative damage in plants. Plants display various defense mechanisms for survival, including the
activation of antioxidant enzymes and the production of low molecular weight non-enzymatic
antioxidants like phenolics. In this work, roots of four weeks old Nepeta nervosa plants were exposed to PEG solutions with osmotic pressure of −3 MPa for one, three and six days. Fresh weight
(FW) and relative water content (RWC) of N. nervosa plants were significantly reduced compared
to the corresponding control - up to 68% (FW) and up to 35% (RWC). In leaves, the content of rosmarinic and chlorogenic acids, dominant phenolic compounds, as well as of less abundant ferulic
and caffeic acids, was dependent on the duration of PEG-induced drought stress. The content of
rosmarinic acid in control plants achieved an average value of 18.27 µg/mg FW, while at PEG-treatments it increased by 355% (3 days of PEG-treatment). Among all examined antioxidative enzymes
in leaves of PEG treated N. nervosa plants, superoxide dismutases (SOD) were the most active (an
increase of 853% on day 6). PEG treatment also caused an increase in peroxidase (POX) activity in
leaves of N. nervosa plants (108% increase on day 3). Since catalase (CAT) activity was significantly decreased on PEG treatment, except on day 1, it could be presumed that H2O2 elimination is
performed mostly by POX. This work is a useful contribution to the development of a strategy for
overcoming oxidative stress in Nepeta species, especially in arid and semiarid regions.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Physiological and antioxidant defense mechanisms of Nepeta nervosa plants against polyethylene glycol (PEG)-mediated drought stress
SP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5570
ER  - 

@conference{
author = "Dmitrović, Slavica and Nestorović Živković, Jasmina and Aničić, Neda and Skorić, Marijana and Gašić, Uroš and Milutinović, Milica and Matekalo, Dragana and Petrović, Luka and Božunović, Jelena and Filipović, Biljana and Banjanac, Tijana and Šiler, Branislav and Todorović, Miloš and Lukić, Tamara and Mišić, Danijela",
year = "2022",
abstract = "Drough-stress conditions lead to excessive production of reactive oxygen species (ROS) and
oxidative damage in plants. Plants display various defense mechanisms for survival, including the
activation of antioxidant enzymes and the production of low molecular weight non-enzymatic
antioxidants like phenolics. In this work, roots of four weeks old Nepeta nervosa plants were exposed to PEG solutions with osmotic pressure of −3 MPa for one, three and six days. Fresh weight
(FW) and relative water content (RWC) of N. nervosa plants were significantly reduced compared
to the corresponding control - up to 68% (FW) and up to 35% (RWC). In leaves, the content of rosmarinic and chlorogenic acids, dominant phenolic compounds, as well as of less abundant ferulic
and caffeic acids, was dependent on the duration of PEG-induced drought stress. The content of
rosmarinic acid in control plants achieved an average value of 18.27 µg/mg FW, while at PEG-treatments it increased by 355% (3 days of PEG-treatment). Among all examined antioxidative enzymes
in leaves of PEG treated N. nervosa plants, superoxide dismutases (SOD) were the most active (an
increase of 853% on day 6). PEG treatment also caused an increase in peroxidase (POX) activity in
leaves of N. nervosa plants (108% increase on day 3). Since catalase (CAT) activity was significantly decreased on PEG treatment, except on day 1, it could be presumed that H2O2 elimination is
performed mostly by POX. This work is a useful contribution to the development of a strategy for
overcoming oxidative stress in Nepeta species, especially in arid and semiarid regions.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Physiological and antioxidant defense mechanisms of Nepeta nervosa plants against polyethylene glycol (PEG)-mediated drought stress",
pages = "68",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5570"
}

Dmitrović, S., Nestorović Živković, J., Aničić, N., Skorić, M., Gašić, U., Milutinović, M., Matekalo, D., Petrović, L., Božunović, J., Filipović, B., Banjanac, T., Šiler, B., Todorović, M., Lukić, T.,& Mišić, D.. (2022). Physiological and antioxidant defense mechanisms of Nepeta nervosa plants against polyethylene glycol (PEG)-mediated drought stress. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 68.
https://hdl.handle.net/21.15107/rcub_ibiss_5570

Dmitrović S, Nestorović Živković J, Aničić N, Skorić M, Gašić U, Milutinović M, Matekalo D, Petrović L, Božunović J, Filipović B, Banjanac T, Šiler B, Todorović M, Lukić T, Mišić D. Physiological and antioxidant defense mechanisms of Nepeta nervosa plants against polyethylene glycol (PEG)-mediated drought stress. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:68.
https://hdl.handle.net/21.15107/rcub_ibiss_5570 .

Dmitrović, Slavica, Nestorović Živković, Jasmina, Aničić, Neda, Skorić, Marijana, Gašić, Uroš, Milutinović, Milica, Matekalo, Dragana, Petrović, Luka, Božunović, Jelena, Filipović, Biljana, Banjanac, Tijana, Šiler, Branislav, Todorović, Miloš, Lukić, Tamara, Mišić, Danijela, "Physiological and antioxidant defense mechanisms of Nepeta nervosa plants against polyethylene glycol (PEG)-mediated drought stress" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):68,
https://hdl.handle.net/21.15107/rcub_ibiss_5570 .

TY  - CONF
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Dmitrović, Slavica
AU  - Anđelković, Boban
AU  - Dimitrijević, Milena
AU  - Dragićević, Milan
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6563
AB  - Nepeta L. is the largest genus of the Lamiaceae family, and the exclusive representative of the
subfamily Nepetoidae which produces iridoids. However, the genus Nepeta comprises both taxa
producing iridoids (iridoid aglycones-IAs and/or iridoid glucosides-IGs), and those lacking iridoids.
Combining omics-guided approach and functional genomics in elucidating the genetic background
of the iridoid diversity within the genus Nepeta, we provide novel insights into molecular
mechanisms driving the iridoid biosynthesis, and thus into chemical evolution of iridoids within
the genus Nepeta. Multidisciplinary experimental approach is here explained on the example
of iridoid synthases (IS), functionally characterized by in vitro enzymatic assays using recombinant
proteins heterologously expressed in Escherichia coli. Functional ISs are expressed in both
iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa
L. (NnIS). N. nervosa possesses an iridoid biosynthetic platform, but inactive one, most likely due
to the “switching off” or “silencing” of some gene(s) upstream in the pathway, which suggests that
this species has lost the ability to produce iridoids during the evolution. Furthermore, comparative
iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs)
during the exposure of chemodiverse Nepeta taxa to environmental stresses (e.g. dehydration,
UV−B radiation, pathogens) or elicitors (MeJA), facilitated the explanation of regulatory mechanisms
determining the productivity of plants, as well as of factors responsible for the presence/
absence of iridoids or specific groups of iridoids (IAs and IGs), in these plants.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights
SP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6563
ER  - 

@conference{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Božunović, Jelena and Nestorović Živković, Jasmina and Gašić, Uroš and Milutinović, Milica and Dmitrović, Slavica and Anđelković, Boban and Dimitrijević, Milena and Dragićević, Milan and Mišić, Danijela",
year = "2022",
abstract = "Nepeta L. is the largest genus of the Lamiaceae family, and the exclusive representative of the
subfamily Nepetoidae which produces iridoids. However, the genus Nepeta comprises both taxa
producing iridoids (iridoid aglycones-IAs and/or iridoid glucosides-IGs), and those lacking iridoids.
Combining omics-guided approach and functional genomics in elucidating the genetic background
of the iridoid diversity within the genus Nepeta, we provide novel insights into molecular
mechanisms driving the iridoid biosynthesis, and thus into chemical evolution of iridoids within
the genus Nepeta. Multidisciplinary experimental approach is here explained on the example
of iridoid synthases (IS), functionally characterized by in vitro enzymatic assays using recombinant
proteins heterologously expressed in Escherichia coli. Functional ISs are expressed in both
iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa
L. (NnIS). N. nervosa possesses an iridoid biosynthetic platform, but inactive one, most likely due
to the “switching off” or “silencing” of some gene(s) upstream in the pathway, which suggests that
this species has lost the ability to produce iridoids during the evolution. Furthermore, comparative
iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs)
during the exposure of chemodiverse Nepeta taxa to environmental stresses (e.g. dehydration,
UV−B radiation, pathogens) or elicitors (MeJA), facilitated the explanation of regulatory mechanisms
determining the productivity of plants, as well as of factors responsible for the presence/
absence of iridoids or specific groups of iridoids (IAs and IGs), in these plants.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights",
pages = "136",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6563"
}

Aničić, N., Matekalo, D., Skorić, M., Božunović, J., Nestorović Živković, J., Gašić, U., Milutinović, M., Dmitrović, S., Anđelković, B., Dimitrijević, M., Dragićević, M.,& Mišić, D.. (2022). Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 136.
https://hdl.handle.net/21.15107/rcub_ibiss_6563

Aničić N, Matekalo D, Skorić M, Božunović J, Nestorović Živković J, Gašić U, Milutinović M, Dmitrović S, Anđelković B, Dimitrijević M, Dragićević M, Mišić D. Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:136.
https://hdl.handle.net/21.15107/rcub_ibiss_6563 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Božunović, Jelena, Nestorović Živković, Jasmina, Gašić, Uroš, Milutinović, Milica, Dmitrović, Slavica, Anđelković, Boban, Dimitrijević, Milena, Dragićević, Milan, Mišić, Danijela, "Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):136,
https://hdl.handle.net/21.15107/rcub_ibiss_6563 .

TY  - CONF
AU  - Gašić, Uroš
AU  - Lukić, Tamara
AU  - Todorović, Miloš
AU  - Petrović, Luka
AU  - Aničić, Neda
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Matekalo, Dragana
AU  - Filipović, Biljana
AU  - Dmitrović, Slavica
AU  - Šiler, Branislav
AU  - Skorić, Marijana
AU  - Živković, Suzana
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5281
AB  - The aim of this research was to determine inter-species diversity in qualitative composition of methanol-soluble metabolites in leaves of three Digitalis species (D. lanata, D. ferruginea, and D. ambigua) from the central Balkans. Using the UHPLC-LTQ OrbiTrap MS technique, a total of 119 compounds were identified based on their monoisotopic masses, MSn fragmentation, and previously reported MS data. All indetified compounds were clustered into seven groups: steroidal glycosides (43 compounds), steroid aglycones (12 compounds), phenylethanoid glycosides (15 compounds), flavonoid glycosides (18 compounds), flavonoid aglycones (9 compounds), phenolic acid derivatives (14 compounds), and 8 compounds belonging to other classes. In addition to the expected cardiac glycosides, steroidal saponins and pregnane and furostanol glycosides were also abundant in the extracts of analysed Digitalis species. A detailed LC/MS qualitative analysis revealed very similar profiles of steroidal glycosides in extracts of D. lanata and D. ferruginea leaves, which were characterized by significant amounts of pharmaceutically important digoxin, deslanatoside, and lanatosides A, B, and C. On the other hand, these compounds were not detected in leaves of D. ambigua. The UHPLC-LTQ OrbiTrap MS method was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic determination and chemical differentiation of Digitalis species. It also alowed the selection of high-resolution chemical markers for revealing inter- and intra-population variability of Digitalis species adopting targeted metabolomic approach, which is the course of our further work.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Metabolomic evaluation of three Digitalis species (fam. Plantaginaceae) using liquid chromatography with multistage mass spectrometry
SP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5281
ER  - 

@conference{
author = "Gašić, Uroš and Lukić, Tamara and Todorović, Miloš and Petrović, Luka and Aničić, Neda and Milutinović, Milica and Božunović, Jelena and Nestorović Živković, Jasmina and Banjanac, Tijana and Matekalo, Dragana and Filipović, Biljana and Dmitrović, Slavica and Šiler, Branislav and Skorić, Marijana and Živković, Suzana and Mišić, Danijela",
year = "2022",
abstract = "The aim of this research was to determine inter-species diversity in qualitative composition of methanol-soluble metabolites in leaves of three Digitalis species (D. lanata, D. ferruginea, and D. ambigua) from the central Balkans. Using the UHPLC-LTQ OrbiTrap MS technique, a total of 119 compounds were identified based on their monoisotopic masses, MSn fragmentation, and previously reported MS data. All indetified compounds were clustered into seven groups: steroidal glycosides (43 compounds), steroid aglycones (12 compounds), phenylethanoid glycosides (15 compounds), flavonoid glycosides (18 compounds), flavonoid aglycones (9 compounds), phenolic acid derivatives (14 compounds), and 8 compounds belonging to other classes. In addition to the expected cardiac glycosides, steroidal saponins and pregnane and furostanol glycosides were also abundant in the extracts of analysed Digitalis species. A detailed LC/MS qualitative analysis revealed very similar profiles of steroidal glycosides in extracts of D. lanata and D. ferruginea leaves, which were characterized by significant amounts of pharmaceutically important digoxin, deslanatoside, and lanatosides A, B, and C. On the other hand, these compounds were not detected in leaves of D. ambigua. The UHPLC-LTQ OrbiTrap MS method was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic determination and chemical differentiation of Digitalis species. It also alowed the selection of high-resolution chemical markers for revealing inter- and intra-population variability of Digitalis species adopting targeted metabolomic approach, which is the course of our further work.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Metabolomic evaluation of three Digitalis species (fam. Plantaginaceae) using liquid chromatography with multistage mass spectrometry",
pages = "149",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5281"
}

Gašić, U., Lukić, T., Todorović, M., Petrović, L., Aničić, N., Milutinović, M., Božunović, J., Nestorović Živković, J., Banjanac, T., Matekalo, D., Filipović, B., Dmitrović, S., Šiler, B., Skorić, M., Živković, S.,& Mišić, D.. (2022). Metabolomic evaluation of three Digitalis species (fam. Plantaginaceae) using liquid chromatography with multistage mass spectrometry. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 149.
https://hdl.handle.net/21.15107/rcub_ibiss_5281

Gašić U, Lukić T, Todorović M, Petrović L, Aničić N, Milutinović M, Božunović J, Nestorović Živković J, Banjanac T, Matekalo D, Filipović B, Dmitrović S, Šiler B, Skorić M, Živković S, Mišić D. Metabolomic evaluation of three Digitalis species (fam. Plantaginaceae) using liquid chromatography with multistage mass spectrometry. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:149.
https://hdl.handle.net/21.15107/rcub_ibiss_5281 .

Gašić, Uroš, Lukić, Tamara, Todorović, Miloš, Petrović, Luka, Aničić, Neda, Milutinović, Milica, Božunović, Jelena, Nestorović Živković, Jasmina, Banjanac, Tijana, Matekalo, Dragana, Filipović, Biljana, Dmitrović, Slavica, Šiler, Branislav, Skorić, Marijana, Živković, Suzana, Mišić, Danijela, "Metabolomic evaluation of three Digitalis species (fam. Plantaginaceae) using liquid chromatography with multistage mass spectrometry" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):149,
https://hdl.handle.net/21.15107/rcub_ibiss_5281 .

TY  - CONF
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Stojković, Dejan
AU  - Dmitrović, Slavica
AU  - Nestorović Živković, Jasmina
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Božunović, Jelena
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5320
AB  - Trichoderma spp. have been extensively explored and used in agriculture due to their well-known biological control mechanisms. Fungi of the genus Trichoderma produce secondary metabolites which affect plant metabolism by stimulating the production of defense-related compounds and increasing the antioxidant capacity through enhanced polyphenol content and elevated activity of antioxidant enzymes, a result of the excess production of reactive oxygen species. In this work, Nepeta sibirica plants were grown in vitro on solid medium treated with two Trichoderma strains (T. viride and T. harzianum). After three and six days of treatment, leaves were metabolically profiled for major phenolic acids (chlorogenic, caffeic, and rosmarinic acid), and iridoids content (cis,trans-nepetalactone and 1,5,9-epideoxyloganic acid), in parallel with the expression analysis of nepetalactone biosynthetic genes and regulatory genetic elements-transcription factors (TFs). Both fungal strains induced changes in phenolic acids production, while only T. harzianum induced elevated levels of iridoids. Biosynthetic genes GPPS, and IS, as well as TF MYC2, were the only genes with expression levels not affected by the treatments with micro fungi. The response of antioxidant enzymes in N. sibirica leaves was also studied. The most active antioxidant enzyme following the infection with T. viride and T. harzianum was peroxidase (POX). Catalase (CAT) and superoxide dismutase (SOD) activity were also affected by the treatments. Results indicate a possibility of using Trichoderma infection in N. sibirica to elicit the production of biologically active defense compounds.
PB  - Belgrade : Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Alterations in specialized metabolism and antioxidant capacity of Nepeta sibirica L. as induced by two Trichoderma sp.
SP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5320
ER  - 

@conference{
author = "Aničić, Neda and Matekalo, Dragana and Stojković, Dejan and Dmitrović, Slavica and Nestorović Živković, Jasmina and Skorić, Marijana and Gašić, Uroš and Milutinović, Milica and Petrović, Luka and Božunović, Jelena and Filipović, Biljana and Banjanac, Tijana and Šiler, Branislav and Todorović, Miloš and Lukić, Tamara and Mišić, Danijela",
year = "2022",
abstract = "Trichoderma spp. have been extensively explored and used in agriculture due to their well-known biological control mechanisms. Fungi of the genus Trichoderma produce secondary metabolites which affect plant metabolism by stimulating the production of defense-related compounds and increasing the antioxidant capacity through enhanced polyphenol content and elevated activity of antioxidant enzymes, a result of the excess production of reactive oxygen species. In this work, Nepeta sibirica plants were grown in vitro on solid medium treated with two Trichoderma strains (T. viride and T. harzianum). After three and six days of treatment, leaves were metabolically profiled for major phenolic acids (chlorogenic, caffeic, and rosmarinic acid), and iridoids content (cis,trans-nepetalactone and 1,5,9-epideoxyloganic acid), in parallel with the expression analysis of nepetalactone biosynthetic genes and regulatory genetic elements-transcription factors (TFs). Both fungal strains induced changes in phenolic acids production, while only T. harzianum induced elevated levels of iridoids. Biosynthetic genes GPPS, and IS, as well as TF MYC2, were the only genes with expression levels not affected by the treatments with micro fungi. The response of antioxidant enzymes in N. sibirica leaves was also studied. The most active antioxidant enzyme following the infection with T. viride and T. harzianum was peroxidase (POX). Catalase (CAT) and superoxide dismutase (SOD) activity were also affected by the treatments. Results indicate a possibility of using Trichoderma infection in N. sibirica to elicit the production of biologically active defense compounds.",
publisher = "Belgrade : Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Alterations in specialized metabolism and antioxidant capacity of Nepeta sibirica L. as induced by two Trichoderma sp.",
pages = "77",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5320"
}

Aničić, N., Matekalo, D., Stojković, D., Dmitrović, S., Nestorović Živković, J., Skorić, M., Gašić, U., Milutinović, M., Petrović, L., Božunović, J., Filipović, B., Banjanac, T., Šiler, B., Todorović, M., Lukić, T.,& Mišić, D.. (2022). Alterations in specialized metabolism and antioxidant capacity of Nepeta sibirica L. as induced by two Trichoderma sp.. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade : Serbian Plant Physiology Society., 77.
https://hdl.handle.net/21.15107/rcub_ibiss_5320

Aničić N, Matekalo D, Stojković D, Dmitrović S, Nestorović Živković J, Skorić M, Gašić U, Milutinović M, Petrović L, Božunović J, Filipović B, Banjanac T, Šiler B, Todorović M, Lukić T, Mišić D. Alterations in specialized metabolism and antioxidant capacity of Nepeta sibirica L. as induced by two Trichoderma sp.. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:77.
https://hdl.handle.net/21.15107/rcub_ibiss_5320 .

Aničić, Neda, Matekalo, Dragana, Stojković, Dejan, Dmitrović, Slavica, Nestorović Živković, Jasmina, Skorić, Marijana, Gašić, Uroš, Milutinović, Milica, Petrović, Luka, Božunović, Jelena, Filipović, Biljana, Banjanac, Tijana, Šiler, Branislav, Todorović, Miloš, Lukić, Tamara, Mišić, Danijela, "Alterations in specialized metabolism and antioxidant capacity of Nepeta sibirica L. as induced by two Trichoderma sp." in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):77,
https://hdl.handle.net/21.15107/rcub_ibiss_5320 .

TY  - CONF
AU  - Banjanac, Tijana
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Aničić, Neda
AU  - Milutinović, Milica
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Šiler, Branislav
AU  - Filipović, Biljana
AU  - Matekalo, Dragana
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Dragićević, Milan
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5576
AB  - Nepeta species (fam. Lamiace, subfam. Nepetoideae, tribe Nepetae) are well known for their
pharmacological properties, biopesticide potential and behavioral effects on cats. Different biological activities of Nepeta species are closely related to their secondary metabolites, among which
iridoids predominate. Research objective of this study was to determine profiles of methanol-soluble iridoids in leaves of greenhouse-grown plants of 10 phylodiverse Nepeta species: N. grandiflora
M. Bieb., N. laevigata (D. Don) Hand.-Mazz., N. nervosa Royle ex Benth., N. stewartiana Diels, N. nuda
L., N. parnassica Heldr. & Sart., N. rtanjensis Diklić & Milojević, N. ernesti-mayeri Diklić & V. Nikolić, N.
cataria L., and N. sibirica L. Untargeted metabolomics approach adopting UHPLC/LTQ Orbitrap MSn
analysis in a negative ionization mode revealed the presence of totally 15 iridoids in samples, of
which 13 were glucosides and two were aglycons of iridoids. Iridoid compounds were identified
based on their monoisotopic masses, MSn
 fragmentation, and previously reported MS data. The
results indicated that the iridoid profiles of the investigated species are very diverse both in terms
of qualitative content of the compounds and their relative abundance in extracts. Among analysed Nepeta species, Balkan Peninsula endemics (N. rtanjensis, N. parnassica, and N. ernesti-mayeri)
and N. cataria, are especially rich in isomers of 1,5,9-epideoxyloganic acid. 5-Deoxylamiol, nepetariaside, nepetanudoside B, and ajugol were detected in majority of analyzed species. The UHPLC-LTQ OrbiTrap MS analysis, in combination with chemometric data processing, has proven to
be an efficient tool for chemical differentiation between Nepeta taxa, and could aid the efforts to
unravel the chemical evolution within the genus.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae)
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5576
ER  - 

@conference{
author = "Banjanac, Tijana and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Aničić, Neda and Milutinović, Milica and Skorić, Marijana and Božunović, Jelena and Petrović, Luka and Šiler, Branislav and Filipović, Biljana and Matekalo, Dragana and Todorović, Miloš and Lukić, Tamara and Dragićević, Milan and Mišić, Danijela",
year = "2022",
abstract = "Nepeta species (fam. Lamiace, subfam. Nepetoideae, tribe Nepetae) are well known for their
pharmacological properties, biopesticide potential and behavioral effects on cats. Different biological activities of Nepeta species are closely related to their secondary metabolites, among which
iridoids predominate. Research objective of this study was to determine profiles of methanol-soluble iridoids in leaves of greenhouse-grown plants of 10 phylodiverse Nepeta species: N. grandiflora
M. Bieb., N. laevigata (D. Don) Hand.-Mazz., N. nervosa Royle ex Benth., N. stewartiana Diels, N. nuda
L., N. parnassica Heldr. & Sart., N. rtanjensis Diklić & Milojević, N. ernesti-mayeri Diklić & V. Nikolić, N.
cataria L., and N. sibirica L. Untargeted metabolomics approach adopting UHPLC/LTQ Orbitrap MSn
analysis in a negative ionization mode revealed the presence of totally 15 iridoids in samples, of
which 13 were glucosides and two were aglycons of iridoids. Iridoid compounds were identified
based on their monoisotopic masses, MSn
 fragmentation, and previously reported MS data. The
results indicated that the iridoid profiles of the investigated species are very diverse both in terms
of qualitative content of the compounds and their relative abundance in extracts. Among analysed Nepeta species, Balkan Peninsula endemics (N. rtanjensis, N. parnassica, and N. ernesti-mayeri)
and N. cataria, are especially rich in isomers of 1,5,9-epideoxyloganic acid. 5-Deoxylamiol, nepetariaside, nepetanudoside B, and ajugol were detected in majority of analyzed species. The UHPLC-LTQ OrbiTrap MS analysis, in combination with chemometric data processing, has proven to
be an efficient tool for chemical differentiation between Nepeta taxa, and could aid the efforts to
unravel the chemical evolution within the genus.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae)",
pages = "151",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5576"
}

Banjanac, T., Gašić, U., Nestorović Živković, J., Dmitrović, S., Aničić, N., Milutinović, M., Skorić, M., Božunović, J., Petrović, L., Šiler, B., Filipović, B., Matekalo, D., Todorović, M., Lukić, T., Dragićević, M.,& Mišić, D.. (2022). Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae). in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 151.
https://hdl.handle.net/21.15107/rcub_ibiss_5576

Banjanac T, Gašić U, Nestorović Živković J, Dmitrović S, Aničić N, Milutinović M, Skorić M, Božunović J, Petrović L, Šiler B, Filipović B, Matekalo D, Todorović M, Lukić T, Dragićević M, Mišić D. Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae). in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:151.
https://hdl.handle.net/21.15107/rcub_ibiss_5576 .

Banjanac, Tijana, Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Aničić, Neda, Milutinović, Milica, Skorić, Marijana, Božunović, Jelena, Petrović, Luka, Šiler, Branislav, Filipović, Biljana, Matekalo, Dragana, Todorović, Miloš, Lukić, Tamara, Dragićević, Milan, Mišić, Danijela, "Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae)" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):151,
https://hdl.handle.net/21.15107/rcub_ibiss_5576 .

TY  - CONF
AU  - Lukić, Tamara
AU  - Banjanac, Tijana
AU  - Jelić, Mihailo
AU  - Brkušanin, Miloš
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Aničić, Neda
AU  - Milutinović, Milica
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Filipović, Biljana
AU  - Matekalo, Dragana
AU  - Todorović, Miloš
AU  - Mišić, Danijela
AU  - Šiler, Branislav
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5574
AB  - Species of the genus Centaurium are highly variable and clinal morphological variations caused
by environmental conditions are very noticeable. Nevertheless, it is also well documented that
species and subspecies of this genus hybridize readily in nature and it is considered that interspecific hybridization may be one of the leading causes of variability within the genus. Earlier studies
indicate that the species C. erythraea Rafn and C. tenuiflorum (Hoffmanns. et Link) Fritsch in areas
with a Mediterranean climate hybridize in nature. The taxonomic status of the hybridogenous
taxon, which probably originated through the hybridization of the mentioned species, here called
“C. ×tenuiflorum”, has not been resolved. Since it has been proven to be a useful genetic marker in
giving evidence of hybridization among several species of the genus Centaurium, the chloroplast
trnL - trnF region was used for the study of hybridization between these particular species. The
results of variation in the trnL - trnF region indicated that the taxon “C. ×tenuiflorum” shared a chloroplast haplogroup with the species C. tenuiflorum, which implies that C. tenuiflorum might be the
donor of chloroplast DNA. In subsequent research, it is necessary to combine the data obtained
from the analysis of the trnL - trnF region with the data that would be obtained from the analysis
of other regions of genomic and/or chloroplast DNA to resolve the status of this potentially hybridogenic taxon.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Chloroplast trnL - trnF region variation in the genus Centaurium Hill as a molecular indicator of natural interspecific hybridization
SP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5574
ER  - 

@conference{
editor = "Milutinović, Milica",
author = "Lukić, Tamara and Banjanac, Tijana and Jelić, Mihailo and Brkušanin, Miloš and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Aničić, Neda and Milutinović, Milica and Skorić, Marijana and Božunović, Jelena and Petrović, Luka and Filipović, Biljana and Matekalo, Dragana and Todorović, Miloš and Mišić, Danijela and Šiler, Branislav",
year = "2022",
abstract = "Species of the genus Centaurium are highly variable and clinal morphological variations caused
by environmental conditions are very noticeable. Nevertheless, it is also well documented that
species and subspecies of this genus hybridize readily in nature and it is considered that interspecific hybridization may be one of the leading causes of variability within the genus. Earlier studies
indicate that the species C. erythraea Rafn and C. tenuiflorum (Hoffmanns. et Link) Fritsch in areas
with a Mediterranean climate hybridize in nature. The taxonomic status of the hybridogenous
taxon, which probably originated through the hybridization of the mentioned species, here called
“C. ×tenuiflorum”, has not been resolved. Since it has been proven to be a useful genetic marker in
giving evidence of hybridization among several species of the genus Centaurium, the chloroplast
trnL - trnF region was used for the study of hybridization between these particular species. The
results of variation in the trnL - trnF region indicated that the taxon “C. ×tenuiflorum” shared a chloroplast haplogroup with the species C. tenuiflorum, which implies that C. tenuiflorum might be the
donor of chloroplast DNA. In subsequent research, it is necessary to combine the data obtained
from the analysis of the trnL - trnF region with the data that would be obtained from the analysis
of other regions of genomic and/or chloroplast DNA to resolve the status of this potentially hybridogenic taxon.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Chloroplast trnL - trnF region variation in the genus Centaurium Hill as a molecular indicator of natural interspecific hybridization",
pages = "148",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5574"
}

Milutinović, M., Lukić, T., Banjanac, T., Jelić, M., Brkušanin, M., Gašić, U., Nestorović Živković, J., Dmitrović, S., Aničić, N., Milutinović, M., Skorić, M., Božunović, J., Petrović, L., Filipović, B., Matekalo, D., Todorović, M., Mišić, D.,& Šiler, B.. (2022). Chloroplast trnL - trnF region variation in the genus Centaurium Hill as a molecular indicator of natural interspecific hybridization. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 148.
https://hdl.handle.net/21.15107/rcub_ibiss_5574

Milutinović M, Lukić T, Banjanac T, Jelić M, Brkušanin M, Gašić U, Nestorović Živković J, Dmitrović S, Aničić N, Milutinović M, Skorić M, Božunović J, Petrović L, Filipović B, Matekalo D, Todorović M, Mišić D, Šiler B. Chloroplast trnL - trnF region variation in the genus Centaurium Hill as a molecular indicator of natural interspecific hybridization. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:148.
https://hdl.handle.net/21.15107/rcub_ibiss_5574 .

Milutinović, Milica, Lukić, Tamara, Banjanac, Tijana, Jelić, Mihailo, Brkušanin, Miloš, Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Aničić, Neda, Milutinović, Milica, Skorić, Marijana, Božunović, Jelena, Petrović, Luka, Filipović, Biljana, Matekalo, Dragana, Todorović, Miloš, Mišić, Danijela, Šiler, Branislav, "Chloroplast trnL - trnF region variation in the genus Centaurium Hill as a molecular indicator of natural interspecific hybridization" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):148,
https://hdl.handle.net/21.15107/rcub_ibiss_5574 .