TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stupar, Sofija
AU  - Stanišić, Mariana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6710
AB  - Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.
PB  - Turkey. Gebze,Turkey: Technical University
C3  - Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
T1  - How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?
SP  - ID-739
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6710
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stupar, Sofija and Stanišić, Mariana",
year = "2024",
abstract = "Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.",
publisher = "Turkey. Gebze,Turkey: Technical University",
journal = "Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey",
title = "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?",
pages = "ID-739",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6710"
}

Smailagić, D., Dragišić Maksimović, J., Marin, M., Banjac, N., Ninković, S., Stupar, S.,& Stanišić, M.. (2024). How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
Turkey. Gebze,Turkey: Technical University., ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710

Smailagić D, Dragišić Maksimović J, Marin M, Banjac N, Ninković S, Stupar S, Stanišić M. How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey. 2024;:ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stupar, Sofija, Stanišić, Mariana, "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?" in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey (2024):ID-739,
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

TY  - CONF
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Ćosić, Tatjana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Motyka, Vaclav
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6112
AB  - Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling
SP  - 484
EP  - 484
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6112
ER  - 

@conference{
author = "Ninković, Slavica and Banjac, Nevena and Stanišić, Mariana and Smailagić, Dijana and Ćosić, Tatjana and Živanović, Branka and Dobrev, Petre I. and Motyka, Vaclav",
year = "2023",
abstract = "Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling",
pages = "484-484",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6112"
}

Ninković, S., Banjac, N., Stanišić, M., Smailagić, D., Ćosić, T., Živanović, B., Dobrev, P. I.,& Motyka, V.. (2023). Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112

Ninković S, Banjac N, Stanišić M, Smailagić D, Ćosić T, Živanović B, Dobrev PI, Motyka V. Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

Ninković, Slavica, Banjac, Nevena, Stanišić, Mariana, Smailagić, Dijana, Ćosić, Tatjana, Živanović, Branka, Dobrev, Petre I., Motyka, Vaclav, "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):484-484,
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Ristić-Đurović, Jasna L.
AU  - Ćirković, Saša
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Stanišić, Mariana
AU  - Zdravković-Korać, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6420
AB  - Flax seeds stored in unfavorable conditions were exposed to static magnetic fields (SMFs)
of 50–350 mT for 20–120 min to overcome low germination and quality. Seed germination increased
slightly with increasing strength of SMF and duration of treatment. Seed germination from 89% to
100% was achieved in SMF treatments of 150 mT (120 min), 200 mT (80–120 mT), 250 mT (60–120 min),
300 mT (40–120 min), and 350 mT (40–120 min). In these treatments, germination was 2.78- to 3.12-fold
higher than in the control after 10 days. Treatments with 350 mT for 100 and 120 min showed the
best results in germination (100%), as well as a 26.81-fold increase in vigor I, 28.69-fold increase in
vigor II, 1-fold increase in chlorophyll a, 0.84-fold in chlorophyll b, 0.46-fold increase in carotenoid
content, and 2.63-fold increase in catalase activity compared to the control after 10 days. Also,
SMF treatment of 350 mT (20–120 min) reduced cell leakage and electrical conductivity by 1-fold
compared to the control. SMF is a healthy, biologically safe, and environmentally friendly treatment
and can be a tool for overcoming problems of low germination and quality of seeds stored under
unfavorable conditions
PB  - Basel: MDPI
T2  - Agriculture
T1  - Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields
IS  - 11
VL  - 13
DO  - 10.3390/agriculture13112120
SP  - 2120
ER  - 

@article{
author = "Ćalić, Dušica and Ristić-Đurović, Jasna L. and Ćirković, Saša and Milojević, Jelena and Belić, Maja and Stanišić, Mariana and Zdravković-Korać, Snežana",
year = "2023",
abstract = "Flax seeds stored in unfavorable conditions were exposed to static magnetic fields (SMFs)
of 50–350 mT for 20–120 min to overcome low germination and quality. Seed germination increased
slightly with increasing strength of SMF and duration of treatment. Seed germination from 89% to
100% was achieved in SMF treatments of 150 mT (120 min), 200 mT (80–120 mT), 250 mT (60–120 min),
300 mT (40–120 min), and 350 mT (40–120 min). In these treatments, germination was 2.78- to 3.12-fold
higher than in the control after 10 days. Treatments with 350 mT for 100 and 120 min showed the
best results in germination (100%), as well as a 26.81-fold increase in vigor I, 28.69-fold increase in
vigor II, 1-fold increase in chlorophyll a, 0.84-fold in chlorophyll b, 0.46-fold increase in carotenoid
content, and 2.63-fold increase in catalase activity compared to the control after 10 days. Also,
SMF treatment of 350 mT (20–120 min) reduced cell leakage and electrical conductivity by 1-fold
compared to the control. SMF is a healthy, biologically safe, and environmentally friendly treatment
and can be a tool for overcoming problems of low germination and quality of seeds stored under
unfavorable conditions",
publisher = "Basel: MDPI",
journal = "Agriculture",
title = "Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields",
number = "11",
volume = "13",
doi = "10.3390/agriculture13112120",
pages = "2120"
}

Ćalić, D., Ristić-Đurović, J. L., Ćirković, S., Milojević, J., Belić, M., Stanišić, M.,& Zdravković-Korać, S.. (2023). Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields. in Agriculture
Basel: MDPI., 13(11), 2120.
https://doi.org/10.3390/agriculture13112120

Ćalić D, Ristić-Đurović JL, Ćirković S, Milojević J, Belić M, Stanišić M, Zdravković-Korać S. Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields. in Agriculture. 2023;13(11):2120.
doi:10.3390/agriculture13112120 .

Ćalić, Dušica, Ristić-Đurović, Jasna L., Ćirković, Saša, Milojević, Jelena, Belić, Maja, Stanišić, Mariana, Zdravković-Korać, Snežana, "Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields" in Agriculture, 13, no. 11 (2023):2120,
https://doi.org/10.3390/agriculture13112120 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Krstić-Milošević, Dijana
AU  - Mijalković, Tatjana
AU  - Petrović, Mirjana
AU  - Ćosić, Tatjana
AU  - Stanišić, Mariana
AU  - Vinterhalter, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5698
AB  - Eruca sativa is known in traditional medicine for its therapeutic effects, while young plants
are used as a salad or green food. Recently, the consumption of rocket has increased considerably,
so it has become very important for breeders. Plant tissue culture provides a platform to overcome
the problems in improving this species. In the present study, an efficient protocol for in vitro shoot
regeneration and propagation of recalcitrant rocket variety Domaća rukola was studied. Murashige
and Skoog (MS, 1962) medium containing 0.1 mg/L BA and frequent subculture over a period
of three weeks proved to be optimal for shoot multiplication with a multiplication index of over 3
and only 8.72% of hyperhydrated shoots without necrosis. Different concentrations of 2,4-D, BA, or
TDZ in combination with NAA, with or without the presence of AgNO3, were tested for de novo
shoot organogenesis (DNSO) from seedling explants. The hypocotyl explants cultured on MS with a
combination of TDZ1.0+NAA0.1+AgNO3 5.0 mg/L regenerated viable shoots with the highest rate
(25.38%) and an average number of 2.18 shoots per regenerating explant. Somatic embryogenesis
from immature zygotic embryos proved to be the best way to regenerate a recalcitrant rocket cultivar.
The highest embryogenic efficiency was achieved in explants cultured on MS medium containing
1.0 mg/L 2,4-D with a frequency of 76.64% and 5.13 mean number of regenerated somatic embryos
per explant, which were further converted into normal plants. Additionally, in vitro-produced rocket
shoots could serve as a possible promising source for the production of flavonoid kaempferol with
proven antioxidant properties.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola
IS  - 5
VL  - 9
DO  - 10.3390/horticulturae9050533
SP  - 533
ER  - 

@article{
author = "Banjac, Nevena and Krstić-Milošević, Dijana and Mijalković, Tatjana and Petrović, Mirjana and Ćosić, Tatjana and Stanišić, Mariana and Vinterhalter, Branka",
year = "2023",
abstract = "Eruca sativa is known in traditional medicine for its therapeutic effects, while young plants
are used as a salad or green food. Recently, the consumption of rocket has increased considerably,
so it has become very important for breeders. Plant tissue culture provides a platform to overcome
the problems in improving this species. In the present study, an efficient protocol for in vitro shoot
regeneration and propagation of recalcitrant rocket variety Domaća rukola was studied. Murashige
and Skoog (MS, 1962) medium containing 0.1 mg/L BA and frequent subculture over a period
of three weeks proved to be optimal for shoot multiplication with a multiplication index of over 3
and only 8.72% of hyperhydrated shoots without necrosis. Different concentrations of 2,4-D, BA, or
TDZ in combination with NAA, with or without the presence of AgNO3, were tested for de novo
shoot organogenesis (DNSO) from seedling explants. The hypocotyl explants cultured on MS with a
combination of TDZ1.0+NAA0.1+AgNO3 5.0 mg/L regenerated viable shoots with the highest rate
(25.38%) and an average number of 2.18 shoots per regenerating explant. Somatic embryogenesis
from immature zygotic embryos proved to be the best way to regenerate a recalcitrant rocket cultivar.
The highest embryogenic efficiency was achieved in explants cultured on MS medium containing
1.0 mg/L 2,4-D with a frequency of 76.64% and 5.13 mean number of regenerated somatic embryos
per explant, which were further converted into normal plants. Additionally, in vitro-produced rocket
shoots could serve as a possible promising source for the production of flavonoid kaempferol with
proven antioxidant properties.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola",
number = "5",
volume = "9",
doi = "10.3390/horticulturae9050533",
pages = "533"
}

Banjac, N., Krstić-Milošević, D., Mijalković, T., Petrović, M., Ćosić, T., Stanišić, M.,& Vinterhalter, B.. (2023). In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola. in Horticulturae
Basel: MDPI., 9(5), 533.
https://doi.org/10.3390/horticulturae9050533

Banjac N, Krstić-Milošević D, Mijalković T, Petrović M, Ćosić T, Stanišić M, Vinterhalter B. In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola. in Horticulturae. 2023;9(5):533.
doi:10.3390/horticulturae9050533 .

Banjac, Nevena, Krstić-Milošević, Dijana, Mijalković, Tatjana, Petrović, Mirjana, Ćosić, Tatjana, Stanišić, Mariana, Vinterhalter, Branka, "In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola" in Horticulturae, 9, no. 5 (2023):533,
https://doi.org/10.3390/horticulturae9050533 . .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Stupar, Sofija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5067
AB  - Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to 
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards 
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and 
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect 
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings 
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well 
as antioxidant enzymes activities were determined spectrophotometrically in treated and control 
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to 
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The 
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93% 
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced 
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin 
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities 
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of 
further investigation.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5067
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Stupar, Sofija and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to 
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards 
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and 
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect 
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings 
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well 
as antioxidant enzymes activities were determined spectrophotometrically in treated and control 
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to 
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The 
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93% 
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced 
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin 
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities 
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of 
further investigation.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5067"
}

Smailagić, D., Dragišić Maksimović, J., Stupar, S., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_5067

Smailagić D, Dragišić Maksimović J, Stupar S, Banjac N, Ninković S, Stanišić M. Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_5067 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Stupar, Sofija, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):73,
https://hdl.handle.net/21.15107/rcub_ibiss_5067 .

TY  - CONF
AU  - Bondokić, Jasna
AU  - Trajković, Milena
AU  - Dragišić Maksimović, Jelena
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5064
AB  - Phloretin is phenolic compound specifically produced in apple tree species and secreted into the soil as a response to biotic and abiotic factors. Since our previous results indicated roots as the primary site of phloretin phytotoxic action, this study focused on assessment of phloretin effects on the root growth of weed (Chenopodium murale and Lotus corniculatus),  crop (Solanum tuberosum) and woody species (Castanea sativa and Malus × domestica Borkh.). Significant decrease in root biomass production in liquid growth medium enriched with 500 µM phloretin was observed both after 2 and 4 weeks of treatment in all tested species. The percentages of roots growth inhibition were as follows: Chenopodium murale (95% and 89,1%), Lotus corniculatus (69,4% and 81,8%), Solanum tuberosum (88,4% and 84,7%), Castanea sativa (85,6% and 100%) and Malus × domestica Borkh. (65,3% and 87,4%, respectively). Total antioxidant capacity (TAC), total phenolic content (TPC), as well as antioxidant enzymes activities were determined spectrophotometrically in treated and control Chenopodium murale roots after 4 weeks of phloretin treatment. TAC increased 44,1% presumably due to elevated TPC (25,8%) in treated roots. However, there were no significant differences in catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) activities in control and treated roots after 4 weeks of treatment. Increased antioxidant activity in treated roots is presumably the consequence of known phloretin antioxidant potential.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5064
ER  - 

@conference{
author = "Bondokić, Jasna and Trajković, Milena and Dragišić Maksimović, Jelena and Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Phloretin is phenolic compound specifically produced in apple tree species and secreted into the soil as a response to biotic and abiotic factors. Since our previous results indicated roots as the primary site of phloretin phytotoxic action, this study focused on assessment of phloretin effects on the root growth of weed (Chenopodium murale and Lotus corniculatus),  crop (Solanum tuberosum) and woody species (Castanea sativa and Malus × domestica Borkh.). Significant decrease in root biomass production in liquid growth medium enriched with 500 µM phloretin was observed both after 2 and 4 weeks of treatment in all tested species. The percentages of roots growth inhibition were as follows: Chenopodium murale (95% and 89,1%), Lotus corniculatus (69,4% and 81,8%), Solanum tuberosum (88,4% and 84,7%), Castanea sativa (85,6% and 100%) and Malus × domestica Borkh. (65,3% and 87,4%, respectively). Total antioxidant capacity (TAC), total phenolic content (TPC), as well as antioxidant enzymes activities were determined spectrophotometrically in treated and control Chenopodium murale roots after 4 weeks of phloretin treatment. TAC increased 44,1% presumably due to elevated TPC (25,8%) in treated roots. However, there were no significant differences in catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) activities in control and treated roots after 4 weeks of treatment. Increased antioxidant activity in treated roots is presumably the consequence of known phloretin antioxidant potential.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5064"
}

Bondokić, J., Trajković, M., Dragišić Maksimović, J., Smailagić, D., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_5064

Bondokić J, Trajković M, Dragišić Maksimović J, Smailagić D, Banjac N, Ninković S, Stanišić M. The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_5064 .

Bondokić, Jasna, Trajković, Milena, Dragišić Maksimović, Jelena, Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):81,
https://hdl.handle.net/21.15107/rcub_ibiss_5064 .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5066
AB  - Phloretin is a phenolic compound unique for apple (Malus × domestica Borkh.) species. Our 
research group recently reported phytotoxic potential of phloretin towards other plant species 
such as model plant Arabidopsis. Since sugars in in vitro growth media could modulate plant 
metabolism, we analyzed phloretin effects on Arabidopsis seedlings grown on the medium with out sucrose and myoinositol (WSM) but enriched with different concentrations of phloretin (0 
to 1500 µM). Phloretin treatment on WSM induced seedlings growth retardation at much lower 
concentrations compared to sucrose and myoinositol enriched medium. Inhibition of primary 
root and leaf growth, and lateral root emergence increased with both phloretin concentration 
and treatment duration (maximal 85.2%, 30.4% and 46.5% at 1500 µM phloretin at 15 days after 
germination - DAG, respectively). Prolonged cultivation (4 weeks) at phloretin concentrations over 
500 µM induced complete growth stagnation and necrosis of the leaves. Transmission electron 
microscopy reviled relocation of chloroplasts within mesophyll cells, shape change into spherical 
form, as well as ultrastructural changes in thylakoid organization in leaves of 10 DAG-old seedlings 
treated with 250 or 500 µM phloretin. Significantly decreased number of starch grains in chloro plasts and chlorophyll a and b content in leaves reflected impaired function of chloroplasts due 
to ultrastructural changes. Phloretin also induced cell wall undulation and folds that resulted in 
irregularly shaped mesophyll cells. The presented study revealed that beside roots that had been 
previously emphasized as primary targets of phloretin action, leaves were also significantly affect ed by phloretin treatment, especially in the aspect of photosynthetic apparatus
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5066
ER  - 

@conference{
author = "Smailagić, Dijana and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Phloretin is a phenolic compound unique for apple (Malus × domestica Borkh.) species. Our 
research group recently reported phytotoxic potential of phloretin towards other plant species 
such as model plant Arabidopsis. Since sugars in in vitro growth media could modulate plant 
metabolism, we analyzed phloretin effects on Arabidopsis seedlings grown on the medium with out sucrose and myoinositol (WSM) but enriched with different concentrations of phloretin (0 
to 1500 µM). Phloretin treatment on WSM induced seedlings growth retardation at much lower 
concentrations compared to sucrose and myoinositol enriched medium. Inhibition of primary 
root and leaf growth, and lateral root emergence increased with both phloretin concentration 
and treatment duration (maximal 85.2%, 30.4% and 46.5% at 1500 µM phloretin at 15 days after 
germination - DAG, respectively). Prolonged cultivation (4 weeks) at phloretin concentrations over 
500 µM induced complete growth stagnation and necrosis of the leaves. Transmission electron 
microscopy reviled relocation of chloroplasts within mesophyll cells, shape change into spherical 
form, as well as ultrastructural changes in thylakoid organization in leaves of 10 DAG-old seedlings 
treated with 250 or 500 µM phloretin. Significantly decreased number of starch grains in chloro plasts and chlorophyll a and b content in leaves reflected impaired function of chloroplasts due 
to ultrastructural changes. Phloretin also induced cell wall undulation and folds that resulted in 
irregularly shaped mesophyll cells. The presented study revealed that beside roots that had been 
previously emphasized as primary targets of phloretin action, leaves were also significantly affect ed by phloretin treatment, especially in the aspect of photosynthetic apparatus",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5066"
}

Smailagić, D., Marin, M., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 72.
https://hdl.handle.net/21.15107/rcub_ibiss_5066

Smailagić D, Marin M, Banjac N, Ninković S, Stanišić M. Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:72.
https://hdl.handle.net/21.15107/rcub_ibiss_5066 .

Smailagić, Dijana, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):72,
https://hdl.handle.net/21.15107/rcub_ibiss_5066 .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Živanović, Branka
AU  - Maksimović, Vuk
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5063
AB  - Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5063
ER  - 

@conference{
author = "Stanišić, Mariana and Smailagić, Dijana and Živanović, Branka and Maksimović, Vuk and Ninković, Slavica and Banjac, Nevena",
year = "2022",
abstract = "Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings",
pages = "44",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5063"
}

Stanišić, M., Smailagić, D., Živanović, B., Maksimović, V., Ninković, S.,& Banjac, N.. (2022). What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063

Stanišić M, Smailagić D, Živanović B, Maksimović V, Ninković S, Banjac N. What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

Stanišić, Mariana, Smailagić, Dijana, Živanović, Branka, Maksimović, Vuk, Ninković, Slavica, Banjac, Nevena, "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):44,
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

TY  - CHAP
AU  - Stanišić, Mariana
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6567
AB  - Phloridzin and phloretin are dihydrochalcones highly specific to domestic apple (Malus × domestica
Borkh.) and other species from genus Malus. Auto-allelopathic potential of these phenolics was recognized in‘Apple Replant Disease’(ARD) characterized by stunted growth, reduced root system and low yields
in apple trees replanted on lands that previously supported apple orchards. Physiological basis of phloridzin and phloretin autotoxicity are ultrastructural and molecular alterations that include increased catalase, peroxidases and superoxide dismutase activities, raised malondialdehyde and proline contents, and
high expression of genes involved in cell defence system. The latest research on model plant Arabidopsis
thaliana indicates phytotoxicity of these dihydrochalcones towards other plant species. Phloretin inhibits
A. thaliana seedlings growth and development inducing agravitropic phenotype and morphological and
ultrastructural malformations in treated seedlings. Altered expression of CDKA1;1, CDKB2;1, CYCA3;1 and
CYCB2;4 cell cycle genes and genes involved in auxin homeostasis (PINs, AUX1, LAX3, ABCBs, TAA1 and YUCs)
and gibberellin biosynthesis (GA20ox2 and GA3ox1) are in the physiological bases of phloretin phytotoxicity. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves
the way for further research of phloretin role in ARD
AB  - Sekundarni metaboliti jabuke (Malus × domestica Borkh.) florizin i floretin su dihidrohalkoni visoko
specifični za vrste roda Malus. Autoalelopatski potencijal ovih fenolnih jedinjenja se ogleda u suzbijanju
rasta klijanca i sadnica zasađenih na mestu prvobitnih voćnjaka jabuke. U osnovi njihove fitotoksičnosti su
ultrastrukturne i molekularne promene koje se ogledaju u povećanju sadržaja malondialdehida, prolina i
aktivnosti antioksidativnih enzima: superoksid-dismutaze, peroksidaza i katalaza, kao i nivoa ekspresije
proteina uključenih u odbrambene mehanizme ćelije. Najnovija istraživanja ukazuju na fitotoksičnost ovih
dihidrohalkona i prema drugim biljnim vrstama, kao npr. korovskoj model biljci Arabidopsis thaliana. Tretman floretinom izaziva značajnu inhibiciju rastenja i razvića klijanaca A. thaliana, poremećaj gravitropskog
odgovora i pojavu morfoloških i ultrastrukturnih malformacija u čijoj osnovi leži promena ekspresije
CDKA1;1, CDKB2;1, CYCA3;1 i CYCB2;4 gena ćelijskog ciklusa, kao i gena uključenih u održanje homeostaze
auksina (PINs, AUX1, LAX3, ABCBs, TAA1 i YUCs) i biosinteze giberelina (GA20ox2 i GA3ox1). Ovo otvara
mogućnost za dalja istraživanja njihovog alelopatskog delovanja ali i potencijalnu primenu kao ekološki
bezbednih bioherbicida
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Trends in Molecular Biology
T1  - Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance
T1  - Apple dihydrochalcones phloridzin and phloretin as novel allelochemicals
IS  - 2
SP  - 223
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6567
ER  - 

@inbook{
author = "Stanišić, Mariana and Ninković, Slavica and Banjac, Nevena",
year = "2022",
abstract = "Phloridzin and phloretin are dihydrochalcones highly specific to domestic apple (Malus × domestica
Borkh.) and other species from genus Malus. Auto-allelopathic potential of these phenolics was recognized in‘Apple Replant Disease’(ARD) characterized by stunted growth, reduced root system and low yields
in apple trees replanted on lands that previously supported apple orchards. Physiological basis of phloridzin and phloretin autotoxicity are ultrastructural and molecular alterations that include increased catalase, peroxidases and superoxide dismutase activities, raised malondialdehyde and proline contents, and
high expression of genes involved in cell defence system. The latest research on model plant Arabidopsis
thaliana indicates phytotoxicity of these dihydrochalcones towards other plant species. Phloretin inhibits
A. thaliana seedlings growth and development inducing agravitropic phenotype and morphological and
ultrastructural malformations in treated seedlings. Altered expression of CDKA1;1, CDKB2;1, CYCA3;1 and
CYCB2;4 cell cycle genes and genes involved in auxin homeostasis (PINs, AUX1, LAX3, ABCBs, TAA1 and YUCs)
and gibberellin biosynthesis (GA20ox2 and GA3ox1) are in the physiological bases of phloretin phytotoxicity. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves
the way for further research of phloretin role in ARD, Sekundarni metaboliti jabuke (Malus × domestica Borkh.) florizin i floretin su dihidrohalkoni visoko
specifični za vrste roda Malus. Autoalelopatski potencijal ovih fenolnih jedinjenja se ogleda u suzbijanju
rasta klijanca i sadnica zasađenih na mestu prvobitnih voćnjaka jabuke. U osnovi njihove fitotoksičnosti su
ultrastrukturne i molekularne promene koje se ogledaju u povećanju sadržaja malondialdehida, prolina i
aktivnosti antioksidativnih enzima: superoksid-dismutaze, peroksidaza i katalaza, kao i nivoa ekspresije
proteina uključenih u odbrambene mehanizme ćelije. Najnovija istraživanja ukazuju na fitotoksičnost ovih
dihidrohalkona i prema drugim biljnim vrstama, kao npr. korovskoj model biljci Arabidopsis thaliana. Tretman floretinom izaziva značajnu inhibiciju rastenja i razvića klijanaca A. thaliana, poremećaj gravitropskog
odgovora i pojavu morfoloških i ultrastrukturnih malformacija u čijoj osnovi leži promena ekspresije
CDKA1;1, CDKB2;1, CYCA3;1 i CYCB2;4 gena ćelijskog ciklusa, kao i gena uključenih u održanje homeostaze
auksina (PINs, AUX1, LAX3, ABCBs, TAA1 i YUCs) i biosinteze giberelina (GA20ox2 i GA3ox1). Ovo otvara
mogućnost za dalja istraživanja njihovog alelopatskog delovanja ali i potencijalnu primenu kao ekološki
bezbednih bioherbicida",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Trends in Molecular Biology",
booktitle = "Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance, Apple dihydrochalcones phloridzin and phloretin as novel allelochemicals",
number = "2",
pages = "223-232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6567"
}

Stanišić, M., Ninković, S.,& Banjac, N.. (2022). Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance. in Trends in Molecular Biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(2), 223-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6567

Stanišić M, Ninković S, Banjac N. Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance. in Trends in Molecular Biology. 2022;(2):223-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6567 .

Stanišić, Mariana, Ninković, Slavica, Banjac, Nevena, "Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance" in Trends in Molecular Biology, no. 2 (2022):223-232,
https://hdl.handle.net/21.15107/rcub_ibiss_6567 .

TY  - JOUR
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Pěnčík, Aleš
AU  - Ćalić, Dušica
AU  - Bogdanović, Milica
AU  - Trajković, Milena
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5065
AB  - Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action
VL  - 13
DO  - 0.3389/fpls.2022.875528
SP  - 875528
ER  - 

@article{
author = "Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Pěnčík, Aleš and Ćalić, Dušica and Bogdanović, Milica and Trajković, Milena and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action",
volume = "13",
doi = "0.3389/fpls.2022.875528",
pages = "875528"
}

Smailagić, D., Banjac, N., Ninković, S., Savić, J., Ćosić, T., Pěnčík, A., Ćalić, D., Bogdanović, M., Trajković, M.,& Stanišić, M.. (2022). New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 13, 875528.
https://doi.org/0.3389/fpls.2022.875528

Smailagić D, Banjac N, Ninković S, Savić J, Ćosić T, Pěnčík A, Ćalić D, Bogdanović M, Trajković M, Stanišić M. New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science. 2022;13:875528.
doi:0.3389/fpls.2022.875528 .

Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Pěnčík, Aleš, Ćalić, Dušica, Bogdanović, Milica, Trajković, Milena, Stanišić, Mariana, "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action" in Frontiers in Plant Science, 13 (2022):875528,
https://doi.org/0.3389/fpls.2022.875528 . .

TY  - JOUR
AU  - Vinterhalter, Dragan
AU  - Savić, Jelena
AU  - Stanišić, Mariana
AU  - Vinterhalter, Branka
AU  - Dobrev, P. I.
AU  - Motyka, V.
PY  - 2020
UR  - https://link.springer.com/article/10.1007/s10725-019-00561-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3533
AB  - Taking advantage of advanced high performance liquid chromatography–electrospray tandem–mass spectrometry (HPLC–ESI–MS/MS), we screened daily changes in concentrations of endogenous phytohormones of in vitro grown potato (Solanum tuberosum L. cv. Désirée) shoot cultures and checked for possible connections between the rhythmicity of endogenous phytohormones and phototropic bending capacity of the same cultures. Studies done under diurnal 16 h light and 8 h darkness (diurnal) and continuous light (CL) conditions showed prominent daily rhythmicity of endogenous phytohormone levels in both light regimes. Phototropic bending in potato is known to be rhythmic only in the diurnal, whereas in CL conditions the bending response is present but without any daily rhythmicity. For all of the studied phytohormone groups significant differences between the diurnal and CL conditions were found. Changes in the concentration of indole auxins, indole-3-acetic acid (IAA) and its catabolite 2-oxindole-3-acetic acid (OxIAA), were the most prominent. Their levels clearly alternated with level of IAA being high in diurnal and OxIAA in CL conditions. Significant concentration changes were also observed for other phytohormones such as cytokinin ribosides, salicylic acid, abscisic acid and phaseic acid. Observed changes in daily phytohormone levels indicate strong and complex involvement of diverse phytohormone groups in realization of the phototropic bending response of potato shoots.
PB  - Springer Netherlands
T2  - Plant Growth Regulation
T1  - Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures
IS  - 161
VL  - 90
DO  - 10.1007/s10725-019-00561-8
SP  - 151
ER  - 

@article{
author = "Vinterhalter, Dragan and Savić, Jelena and Stanišić, Mariana and Vinterhalter, Branka and Dobrev, P. I. and Motyka, V.",
year = "2020",
abstract = "Taking advantage of advanced high performance liquid chromatography–electrospray tandem–mass spectrometry (HPLC–ESI–MS/MS), we screened daily changes in concentrations of endogenous phytohormones of in vitro grown potato (Solanum tuberosum L. cv. Désirée) shoot cultures and checked for possible connections between the rhythmicity of endogenous phytohormones and phototropic bending capacity of the same cultures. Studies done under diurnal 16 h light and 8 h darkness (diurnal) and continuous light (CL) conditions showed prominent daily rhythmicity of endogenous phytohormone levels in both light regimes. Phototropic bending in potato is known to be rhythmic only in the diurnal, whereas in CL conditions the bending response is present but without any daily rhythmicity. For all of the studied phytohormone groups significant differences between the diurnal and CL conditions were found. Changes in the concentration of indole auxins, indole-3-acetic acid (IAA) and its catabolite 2-oxindole-3-acetic acid (OxIAA), were the most prominent. Their levels clearly alternated with level of IAA being high in diurnal and OxIAA in CL conditions. Significant concentration changes were also observed for other phytohormones such as cytokinin ribosides, salicylic acid, abscisic acid and phaseic acid. Observed changes in daily phytohormone levels indicate strong and complex involvement of diverse phytohormone groups in realization of the phototropic bending response of potato shoots.",
publisher = "Springer Netherlands",
journal = "Plant Growth Regulation",
title = "Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures",
number = "161",
volume = "90",
doi = "10.1007/s10725-019-00561-8",
pages = "151"
}

Vinterhalter, D., Savić, J., Stanišić, M., Vinterhalter, B., Dobrev, P. I.,& Motyka, V.. (2020). Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures. in Plant Growth Regulation
Springer Netherlands., 90(161), 151.
https://doi.org/10.1007/s10725-019-00561-8

Vinterhalter D, Savić J, Stanišić M, Vinterhalter B, Dobrev PI, Motyka V. Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures. in Plant Growth Regulation. 2020;90(161):151.
doi:10.1007/s10725-019-00561-8 .

Vinterhalter, Dragan, Savić, Jelena, Stanišić, Mariana, Vinterhalter, Branka, Dobrev, P. I., Motyka, V., "Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures" in Plant Growth Regulation, 90, no. 161 (2020):151,
https://doi.org/10.1007/s10725-019-00561-8 . .

TY  - THES
AU  - Stanišić, Mariana
PY  - 2019
UR  - http://uvidok.rcub.bg.ac.rs/handle/123456789/3110
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3248
AB  - Ograniĉenja u izuĉavanju potencijala i mehanizama alelopatskog delovanja glavnih sekundarnih metabolita korena jabuke, floretina i florizina, usled kompleksnosti interakcija u rizosferi, mogu biti prevaziĊena upotrebom kulture transformisanih korenova in vitro. Odabranim naĉinom inokulacije regenerisanih izdanaka jabuke sa Agrobacterium rhizogenes 15834, efikasno su indukovani transformisani korenovi kod sorti Melrouz, Zlatni Delińes, Ĉadel i Gloster. Smenom hranljivih podloga sa i bez auksina, po prvi put je uspostavljena permanentna in vitro kultura izolovanih transformisanih korenova jabuke. UHPLC/(+/-)HESI–MS/MS analizom je utvrĊeno da genetiĉka transformacija nije dovela do promena u sadrņaju glavnih sekundarnih metabolita korena jabuke. U eksudatu transformisanih korenova, sadrņanom u teĉnoj podlozi u kojoj su korenovi gajeni, kvantifikovani su iskljuĉivo florizin, floretin, hlorogena i kafeinska kiselina. Tretman ovim eksudatom je prouzrokovao znaĉajnu inhibiciju rastenja i razvića izdanaka i korenova klijanaca Arabidopsis thaliana. Sliĉan inhibitorni efekat ispoljili su ĉist florizin u koncentraciji od 500 μM, kao i floretin u svim testiranim koncentracijama (125-500 μM), izazivajući krupne promene u morfologiji tretiranih klijanaca, posebno korena. Velike sliĉnosti su zapaņene u obrascu promena ekspresije gena ukljuĉenih u polarni transport auksina, kao i biosintezu auksina i giberelina u korenovima klijanaca arabidopsisa tretiranih eksudatom transformisanih korenova jabuke, sa onim detektovanim kod klijanaca tretiranih 500 μM floretinom. To ukazuje na mogućnost da je floretin u eksudatu korena jabuke metabolit koji je odgovoran za alelopatske efekte, a koji bi se mogli zasnivati na promenama nivoa auksina i giberelina, kao i na interakciji ove dve grupe hormona.
AB  - The investigation of allelopathic potential and mode of action of the main apple root secondary metabolites, phloridzin and phloretin is difficult to perform due to high complexity of rhizosphere interactions. Therefore, the generation of in vitro hairy root cultures could be an alternative system for allelopathic studies of apple. The efficient induction of hairy roots in apple cvs. Melrose, Golden Delicious, Ĉadel and Gloster was obtained using the appropriate mode of inoculation of regenerated shoots by Agrobacterium rhizogenes 15834. Permanent in vitro culture of apple autonomous hairy roots was established for the first time by cultivation on alternating growth media with and without auxins. UHPLC(+/-)HESI-MS/MS analysis confirmed that genetic transformation did not lead to changes in the content of the main secondary metabolites of apple roots. Dihydrochalcones phloridzin and phloretin, chlorogenic and caffeic acids were detected as only putative allelochemicals exuded into the growth medium in which hairy roots were grown. Apple hairy root exudates significantly affected development and growth of Arabidopsis thaliana seedlings. Pure phloridzin at 500 μM and phloretin in all tested concentrations (125-500 μM) had similar effects as apple hairy root exudates inducing significant alterations in morphology of seedlings, expecially roots. High similarities in the expression patterns of genes involved in polar auxin transport and auxin and gibberellin biosynthetic pathways in roots of arabidopsis seedlings treated with either apple hairy root exudates or 500 μM phloretin were observed. This indicates that phloretin in hairy root exudates could be at least partly responsible for the apple root allelopathic properties, that were most likely based on auxin and gibberellin cross-talk and their altered homeostasis in arabidopsis roots.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Alelopatski potencijal sekundarnih metabolita transformisanih korenova jabuke - efekat floretina i florizina u kulturi in vitro
T1  - Allelopathic potential of apple hairy root secondary metabolites - the effect of phloretin and phloridzin in in vitro culture
SP  - 1
EP  - 262
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3248
ER  - 

@phdthesis{
author = "Stanišić, Mariana",
year = "2019",
abstract = "Ograniĉenja u izuĉavanju potencijala i mehanizama alelopatskog delovanja glavnih sekundarnih metabolita korena jabuke, floretina i florizina, usled kompleksnosti interakcija u rizosferi, mogu biti prevaziĊena upotrebom kulture transformisanih korenova in vitro. Odabranim naĉinom inokulacije regenerisanih izdanaka jabuke sa Agrobacterium rhizogenes 15834, efikasno su indukovani transformisani korenovi kod sorti Melrouz, Zlatni Delińes, Ĉadel i Gloster. Smenom hranljivih podloga sa i bez auksina, po prvi put je uspostavljena permanentna in vitro kultura izolovanih transformisanih korenova jabuke. UHPLC/(+/-)HESI–MS/MS analizom je utvrĊeno da genetiĉka transformacija nije dovela do promena u sadrņaju glavnih sekundarnih metabolita korena jabuke. U eksudatu transformisanih korenova, sadrņanom u teĉnoj podlozi u kojoj su korenovi gajeni, kvantifikovani su iskljuĉivo florizin, floretin, hlorogena i kafeinska kiselina. Tretman ovim eksudatom je prouzrokovao znaĉajnu inhibiciju rastenja i razvića izdanaka i korenova klijanaca Arabidopsis thaliana. Sliĉan inhibitorni efekat ispoljili su ĉist florizin u koncentraciji od 500 μM, kao i floretin u svim testiranim koncentracijama (125-500 μM), izazivajući krupne promene u morfologiji tretiranih klijanaca, posebno korena. Velike sliĉnosti su zapaņene u obrascu promena ekspresije gena ukljuĉenih u polarni transport auksina, kao i biosintezu auksina i giberelina u korenovima klijanaca arabidopsisa tretiranih eksudatom transformisanih korenova jabuke, sa onim detektovanim kod klijanaca tretiranih 500 μM floretinom. To ukazuje na mogućnost da je floretin u eksudatu korena jabuke metabolit koji je odgovoran za alelopatske efekte, a koji bi se mogli zasnivati na promenama nivoa auksina i giberelina, kao i na interakciji ove dve grupe hormona., The investigation of allelopathic potential and mode of action of the main apple root secondary metabolites, phloridzin and phloretin is difficult to perform due to high complexity of rhizosphere interactions. Therefore, the generation of in vitro hairy root cultures could be an alternative system for allelopathic studies of apple. The efficient induction of hairy roots in apple cvs. Melrose, Golden Delicious, Ĉadel and Gloster was obtained using the appropriate mode of inoculation of regenerated shoots by Agrobacterium rhizogenes 15834. Permanent in vitro culture of apple autonomous hairy roots was established for the first time by cultivation on alternating growth media with and without auxins. UHPLC(+/-)HESI-MS/MS analysis confirmed that genetic transformation did not lead to changes in the content of the main secondary metabolites of apple roots. Dihydrochalcones phloridzin and phloretin, chlorogenic and caffeic acids were detected as only putative allelochemicals exuded into the growth medium in which hairy roots were grown. Apple hairy root exudates significantly affected development and growth of Arabidopsis thaliana seedlings. Pure phloridzin at 500 μM and phloretin in all tested concentrations (125-500 μM) had similar effects as apple hairy root exudates inducing significant alterations in morphology of seedlings, expecially roots. High similarities in the expression patterns of genes involved in polar auxin transport and auxin and gibberellin biosynthetic pathways in roots of arabidopsis seedlings treated with either apple hairy root exudates or 500 μM phloretin were observed. This indicates that phloretin in hairy root exudates could be at least partly responsible for the apple root allelopathic properties, that were most likely based on auxin and gibberellin cross-talk and their altered homeostasis in arabidopsis roots.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Alelopatski potencijal sekundarnih metabolita transformisanih korenova jabuke - efekat floretina i florizina u kulturi in vitro, Allelopathic potential of apple hairy root secondary metabolites - the effect of phloretin and phloridzin in in vitro culture",
pages = "1-262",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3248"
}

Stanišić, M.. (2019). Alelopatski potencijal sekundarnih metabolita transformisanih korenova jabuke - efekat floretina i florizina u kulturi in vitro. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-262.
https://hdl.handle.net/21.15107/rcub_ibiss_3248

Stanišić M. Alelopatski potencijal sekundarnih metabolita transformisanih korenova jabuke - efekat floretina i florizina u kulturi in vitro. in University of Belgrade, Faculty of Biology. 2019;:1-262.
https://hdl.handle.net/21.15107/rcub_ibiss_3248 .

Stanišić, Mariana, "Alelopatski potencijal sekundarnih metabolita transformisanih korenova jabuke - efekat floretina i florizina u kulturi in vitro" in University of Belgrade, Faculty of Biology (2019):1-262,
https://hdl.handle.net/21.15107/rcub_ibiss_3248 .

TY  - JOUR
AU  - Stanišić, Mariana
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Krstić Milošević, Dijana
AU  - Mišić, Danijela
AU  - Smigocki, Ann
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3791
AB  - Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequently
use the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficult
to perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root cultures
of apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation of
apple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain
15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be used
as a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidate
the possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,
kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected in
untransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry
(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production in
apple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicals
exuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantly
affected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days of
treatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsis
shoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanent
in vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.
PB  - Oxford:Oxford University Press
T2  - Tree Physiology
T1  - Hairy root culture as a valuable tool for allelopathic studies in apple
IS  - 5
VL  - 39
DO  - doi:10.1093/treephys/tpz006
SP  - 888
EP  - 905
ER  - 

@article{
author = "Stanišić, Mariana and Ćosić, Tatjana and Savić, Jelena and Krstić Milošević, Dijana and Mišić, Danijela and Smigocki, Ann and Ninković, Slavica and Banjac, Nevena",
year = "2019",
abstract = "Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequently
use the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficult
to perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root cultures
of apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation of
apple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain
15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be used
as a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidate
the possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,
kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected in
untransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry
(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production in
apple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicals
exuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantly
affected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days of
treatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsis
shoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanent
in vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.",
publisher = "Oxford:Oxford University Press",
journal = "Tree Physiology",
title = "Hairy root culture as a valuable tool for allelopathic studies in apple",
number = "5",
volume = "39",
doi = "doi:10.1093/treephys/tpz006",
pages = "888-905"
}

Stanišić, M., Ćosić, T., Savić, J., Krstić Milošević, D., Mišić, D., Smigocki, A., Ninković, S.,& Banjac, N.. (2019). Hairy root culture as a valuable tool for allelopathic studies in apple. in Tree Physiology
Oxford:Oxford University Press., 39(5), 888-905.
https://doi.org/doi:10.1093/treephys/tpz006

Stanišić M, Ćosić T, Savić J, Krstić Milošević D, Mišić D, Smigocki A, Ninković S, Banjac N. Hairy root culture as a valuable tool for allelopathic studies in apple. in Tree Physiology. 2019;39(5):888-905.
doi:doi:10.1093/treephys/tpz006 .

Stanišić, Mariana, Ćosić, Tatjana, Savić, Jelena, Krstić Milošević, Dijana, Mišić, Danijela, Smigocki, Ann, Ninković, Slavica, Banjac, Nevena, "Hairy root culture as a valuable tool for allelopathic studies in apple" in Tree Physiology, 39, no. 5 (2019):888-905,
https://doi.org/doi:10.1093/treephys/tpz006 . .

TY  - JOUR
AU  - Stanišić, Mariana
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Krstić Milošević, Dijana
AU  - Mišić, Danijela
AU  - Smigocki, Ann
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3791
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3792
AB  - Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequentlyuse the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficultto perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root culturesof apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation ofapple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be usedas a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidatethe possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected inuntransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production inapple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicalsexuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantlyaffected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days oftreatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsisshoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanentin vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.
PB  - Oxford: Oxford University Press
T2  - Tree Physiology
T1  - Hairy root culture as a valuable tool for allelopathic studies in apple
IS  - 5
VL  - 39
DO  - doi:10.1093/treephys/tpz006
SP  - 888
EP  - 905
ER  - 

@article{
author = "Stanišić, Mariana and Ćosić, Tatjana and Savić, Jelena and Krstić Milošević, Dijana and Mišić, Danijela and Smigocki, Ann and Ninković, Slavica and Banjac, Nevena",
year = "2019",
abstract = "Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequentlyuse the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficultto perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root culturesof apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation ofapple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be usedas a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidatethe possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected inuntransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production inapple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicalsexuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantlyaffected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days oftreatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsisshoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanentin vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.",
publisher = "Oxford: Oxford University Press",
journal = "Tree Physiology",
title = "Hairy root culture as a valuable tool for allelopathic studies in apple",
number = "5",
volume = "39",
doi = "doi:10.1093/treephys/tpz006",
pages = "888-905"
}

Stanišić, M., Ćosić, T., Savić, J., Krstić Milošević, D., Mišić, D., Smigocki, A., Ninković, S.,& Banjac, N.. (2019). Hairy root culture as a valuable tool for allelopathic studies in apple. in Tree Physiology
Oxford: Oxford University Press., 39(5), 888-905.
https://doi.org/doi:10.1093/treephys/tpz006

Stanišić M, Ćosić T, Savić J, Krstić Milošević D, Mišić D, Smigocki A, Ninković S, Banjac N. Hairy root culture as a valuable tool for allelopathic studies in apple. in Tree Physiology. 2019;39(5):888-905.
doi:doi:10.1093/treephys/tpz006 .

Stanišić, Mariana, Ćosić, Tatjana, Savić, Jelena, Krstić Milošević, Dijana, Mišić, Danijela, Smigocki, Ann, Ninković, Slavica, Banjac, Nevena, "Hairy root culture as a valuable tool for allelopathic studies in apple" in Tree Physiology, 39, no. 5 (2019):888-905,
https://doi.org/doi:10.1093/treephys/tpz006 . .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Krstić Milošević, Dijana
AU  - Mišić, Danijela
AU  - Smigocki, Ann
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5009
AB  - Since the investigation of plant allelopathic interactions in the rhizosphere is difficult to perform
because of its high complexity, microbial and environmental factors interactions, the generation
of autonomous root culture could be appropriate system for the studies of allelopathic potential of apple (Malus x domestica Borkh.). Thus, we have focused our efforts on the generation
of transformed hairy-roots in apple cv. Golden Delicious using the 15834 strain of Agrobacterium
rhizogenes. The agrobacteria inoculation by dipping the basal cut off end of the stem of in vitro
grown apple shoots into bacterial suspension proved to be the best option for transformation,
providing efficiency of 39.3%. Molecular characterization of hairy roots by RT-PCR analyses confirmed
their transgenic nature. In vitro conditions for apple hairy root growth and maintenance
were optimized using different mineral solutions and auxins. The liquid half-strength MS medium
with the addition of IBA at 0.025 mg L-1 was chosen for the initial stimulation of growth. For their
prolonged cultivation and maintenance, the alternation of IBA-containing and IBA-free media is
recommendable. Fast-growing lines continuously maintained in IBA-free medium were used for
further research. Potential allelochemicals including phenolic acids (chlorogenic, caffeic, syringic,
p-coumaric and ferulic), glycosilated flavonoids (phloridzin, rutin, quercitrin, isoquercitrin, kaempferol-
3-glucoside) and flavonoid aglycons (floretin, quercetin and naringenin) have been detected
by UHPLC/DAD/(+/-)HESI-MS/MS analyses in the growth medium, in which these transformed
roots were maintained for 4 weeks, as well as in the root tissue itself. The obtained hairy-root
cultures offer a complementary system for studying and validating the allelopathic potential of
apple roots.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Transgenic hairy root in vitro culture system of apple as a tool for allelopathic studies
SP  - 55
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5009
ER  - 

@conference{
author = "Stanišić, Mariana and Ćosić, Tatjana and Savić, Jelena and Krstić Milošević, Dijana and Mišić, Danijela and Smigocki, Ann and Ninković, Slavica and Banjac, Nevena",
year = "2018",
abstract = "Since the investigation of plant allelopathic interactions in the rhizosphere is difficult to perform
because of its high complexity, microbial and environmental factors interactions, the generation
of autonomous root culture could be appropriate system for the studies of allelopathic potential of apple (Malus x domestica Borkh.). Thus, we have focused our efforts on the generation
of transformed hairy-roots in apple cv. Golden Delicious using the 15834 strain of Agrobacterium
rhizogenes. The agrobacteria inoculation by dipping the basal cut off end of the stem of in vitro
grown apple shoots into bacterial suspension proved to be the best option for transformation,
providing efficiency of 39.3%. Molecular characterization of hairy roots by RT-PCR analyses confirmed
their transgenic nature. In vitro conditions for apple hairy root growth and maintenance
were optimized using different mineral solutions and auxins. The liquid half-strength MS medium
with the addition of IBA at 0.025 mg L-1 was chosen for the initial stimulation of growth. For their
prolonged cultivation and maintenance, the alternation of IBA-containing and IBA-free media is
recommendable. Fast-growing lines continuously maintained in IBA-free medium were used for
further research. Potential allelochemicals including phenolic acids (chlorogenic, caffeic, syringic,
p-coumaric and ferulic), glycosilated flavonoids (phloridzin, rutin, quercitrin, isoquercitrin, kaempferol-
3-glucoside) and flavonoid aglycons (floretin, quercetin and naringenin) have been detected
by UHPLC/DAD/(+/-)HESI-MS/MS analyses in the growth medium, in which these transformed
roots were maintained for 4 weeks, as well as in the root tissue itself. The obtained hairy-root
cultures offer a complementary system for studying and validating the allelopathic potential of
apple roots.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Transgenic hairy root in vitro culture system of apple as a tool for allelopathic studies",
pages = "55-56",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5009"
}

Stanišić, M., Ćosić, T., Savić, J., Krstić Milošević, D., Mišić, D., Smigocki, A., Ninković, S.,& Banjac, N.. (2018). Transgenic hairy root in vitro culture system of apple as a tool for allelopathic studies. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 55-56.
https://hdl.handle.net/21.15107/rcub_ibiss_5009

Stanišić M, Ćosić T, Savić J, Krstić Milošević D, Mišić D, Smigocki A, Ninković S, Banjac N. Transgenic hairy root in vitro culture system of apple as a tool for allelopathic studies. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:55-56.
https://hdl.handle.net/21.15107/rcub_ibiss_5009 .

Stanišić, Mariana, Ćosić, Tatjana, Savić, Jelena, Krstić Milošević, Dijana, Mišić, Danijela, Smigocki, Ann, Ninković, Slavica, Banjac, Nevena, "Transgenic hairy root in vitro culture system of apple as a tool for allelopathic studies" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):55-56,
https://hdl.handle.net/21.15107/rcub_ibiss_5009 .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Smigocki, Ann
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4999
AB  - In the previous study, phenolic acids and polyphenolic flavonoids were identified as putative
allelochemicals in apple cv. Golden Delicious hairy roots growth medium. In order to examine
their allelopathic potential, the effect of hairy root exudates on the germination and seedling
growth of the test species Arabidopsis thaliana was elucidated. Arabidopsis seeds were cultivated
in Erlenmeyer flasks containing 25 mL of liquid medium in which apple hairy roots were cultivated
for 4 weeks. The effect of apple hairy root growth medium on Arabidopsis was evaluated after 5
and 10 days of seed cultivation. Apple hairy root exudates had no significant effect on Arabidopsis
germination rate, while growth characteristics of seedlings were significantly affected both after
5 and 10 days of treatment. The roots of treated seedlings were significantly shorter (35.1%), with
lower number (74% decrement) of lateral roots compared to control after10 days of treatment.
The growth and morphology of Arabidopsis shoots and leaves have been affected too. Thus, the
shoots of treated seedlings were almost twice shorter compared to control after 5 days of treatment. At the 10th day, this distinction became more pronounced, when two fully expanded leaves
were visible in treated seedlings, while control had completely developed rosettes with six leaves.
In addition, leaves of treated seedlings were smaller and pale in colour compared to control. qPCR
expression analysis of genes involved in regulation of the cell cycle showed reduction of transcript
levels in roots of treated seedlings, with the highest expression decrease detected for CDKA1 and
CYCB2;4 cyclin.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade
T1  - Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro
SP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4999
ER  - 

@conference{
author = "Stanišić, Mariana and Ćosić, Tatjana and Savić, Jelena and Smigocki, Ann and Ninković, Slavica and Banjac, Nevena",
year = "2018",
abstract = "In the previous study, phenolic acids and polyphenolic flavonoids were identified as putative
allelochemicals in apple cv. Golden Delicious hairy roots growth medium. In order to examine
their allelopathic potential, the effect of hairy root exudates on the germination and seedling
growth of the test species Arabidopsis thaliana was elucidated. Arabidopsis seeds were cultivated
in Erlenmeyer flasks containing 25 mL of liquid medium in which apple hairy roots were cultivated
for 4 weeks. The effect of apple hairy root growth medium on Arabidopsis was evaluated after 5
and 10 days of seed cultivation. Apple hairy root exudates had no significant effect on Arabidopsis
germination rate, while growth characteristics of seedlings were significantly affected both after
5 and 10 days of treatment. The roots of treated seedlings were significantly shorter (35.1%), with
lower number (74% decrement) of lateral roots compared to control after10 days of treatment.
The growth and morphology of Arabidopsis shoots and leaves have been affected too. Thus, the
shoots of treated seedlings were almost twice shorter compared to control after 5 days of treatment. At the 10th day, this distinction became more pronounced, when two fully expanded leaves
were visible in treated seedlings, while control had completely developed rosettes with six leaves.
In addition, leaves of treated seedlings were smaller and pale in colour compared to control. qPCR
expression analysis of genes involved in regulation of the cell cycle showed reduction of transcript
levels in roots of treated seedlings, with the highest expression decrease detected for CDKA1 and
CYCB2;4 cyclin.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade",
title = "Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro",
pages = "55",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4999"
}

Stanišić, M., Ćosić, T., Savić, J., Smigocki, A., Ninković, S.,& Banjac, N.. (2018). Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro. in 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade
Belgrade: Serbian Plant Physiology Society., 55.
https://hdl.handle.net/21.15107/rcub_ibiss_4999

Stanišić M, Ćosić T, Savić J, Smigocki A, Ninković S, Banjac N. Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro. in 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade. 2018;:55.
https://hdl.handle.net/21.15107/rcub_ibiss_4999 .

Stanišić, Mariana, Ćosić, Tatjana, Savić, Jelena, Smigocki, Ann, Ninković, Slavica, Banjac, Nevena, "Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro" in 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade (2018):55,
https://hdl.handle.net/21.15107/rcub_ibiss_4999 .

TY  - JOUR
AU  - Stanišić, Mariana
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Banjac, Nevena
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700037S
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3018
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2022
AB  - Since the genetic transformation of the apple is strongly genotype-dependent and generally inefficient, the evaluation of factors affecting shoot regeneration are crucial for the establishment of a successful transformation process. In this report, we evaluated the effects of the β-lactam antibiotics meropenem and timentin on in vitro regeneration via de novo shoot organogenesis from leaf explants of apple cv. Golden Delicious, as well as on the growth of the Agrobacterium tumefaciens strain EHA 105, and compared them with the commonly used β-lactam cefotaxime. Also, we report for the first time the effect of hygromycin B as a selective agent in the domesticated apple, as regards shoot regeneration and shoot multiplication efficiency. We observed that cefotaxime and timentin at concentrations higher than 100 mg L-1 were sufficient to prevent Agrobacterium growth during a two-week period, while meropenem exhibited an inhibitory effect on bacterial growth at all tested concentrations (25-150 mg L-1). Cefotaxime at a concentration of 300 mg L-1 increased the number of regenerated shoots per explant (9.39) in comparison with the control (7.67). In contrast to cefotaxime, meropenem and timentin caused a decrease in shoot regeneration efficiency, but larger and more developed shoots were obtained on meropenem (25-125 mg L-1) after the same period of cultivation. Hygromycin B at a concentration of 5 mg L-1 or higher completely inhibited shoot regeneration and induced explant tissue necrosis. Therefore, the selection procedure with a final concentration of 4 mg L-1 throughout organogenesis and 10 mg L-1 for further shoot growth and multiplication is recommended for an efficient transformation process in apple cv. Golden Delicious.
T2  - Archives of Biological Sciences
T1  - The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious
IS  - 1
VL  - 70
DO  - 10.2298/ABS170731037S
SP  - 179
EP  - 190
ER  - 

@article{
author = "Stanišić, Mariana and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Banjac, Nevena",
year = "2018",
abstract = "Since the genetic transformation of the apple is strongly genotype-dependent and generally inefficient, the evaluation of factors affecting shoot regeneration are crucial for the establishment of a successful transformation process. In this report, we evaluated the effects of the β-lactam antibiotics meropenem and timentin on in vitro regeneration via de novo shoot organogenesis from leaf explants of apple cv. Golden Delicious, as well as on the growth of the Agrobacterium tumefaciens strain EHA 105, and compared them with the commonly used β-lactam cefotaxime. Also, we report for the first time the effect of hygromycin B as a selective agent in the domesticated apple, as regards shoot regeneration and shoot multiplication efficiency. We observed that cefotaxime and timentin at concentrations higher than 100 mg L-1 were sufficient to prevent Agrobacterium growth during a two-week period, while meropenem exhibited an inhibitory effect on bacterial growth at all tested concentrations (25-150 mg L-1). Cefotaxime at a concentration of 300 mg L-1 increased the number of regenerated shoots per explant (9.39) in comparison with the control (7.67). In contrast to cefotaxime, meropenem and timentin caused a decrease in shoot regeneration efficiency, but larger and more developed shoots were obtained on meropenem (25-125 mg L-1) after the same period of cultivation. Hygromycin B at a concentration of 5 mg L-1 or higher completely inhibited shoot regeneration and induced explant tissue necrosis. Therefore, the selection procedure with a final concentration of 4 mg L-1 throughout organogenesis and 10 mg L-1 for further shoot growth and multiplication is recommended for an efficient transformation process in apple cv. Golden Delicious.",
journal = "Archives of Biological Sciences",
title = "The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious",
number = "1",
volume = "70",
doi = "10.2298/ABS170731037S",
pages = "179-190"
}

Stanišić, M., Ninković, S., Savić, J., Ćosić, T.,& Banjac, N.. (2018). The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious. in Archives of Biological Sciences, 70(1), 179-190.
https://doi.org/10.2298/ABS170731037S

Stanišić M, Ninković S, Savić J, Ćosić T, Banjac N. The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious. in Archives of Biological Sciences. 2018;70(1):179-190.
doi:10.2298/ABS170731037S .

Stanišić, Mariana, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Banjac, Nevena, "The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious" in Archives of Biological Sciences, 70, no. 1 (2018):179-190,
https://doi.org/10.2298/ABS170731037S . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Stanisavljević, Nemanja
AU  - Miljuš-Đukić, Jovanka
AU  - Marin, Marija
AU  - Radović, Svetlana
AU  - Ninković, Slavica
PY  - 2018
UR  - http://link.springer.com/10.1007/s00709-018-1250-0
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3064
AB  - Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataša seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.
T2  - Protoplasma
T1  - Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.
DO  - 10.1007/s00709-018-1250-0
ER  - 

@article{
author = "Banjac, Nevena and Stanišić, Mariana and Savić, Jelena and Ćosić, Tatjana and Stanisavljević, Nemanja and Miljuš-Đukić, Jovanka and Marin, Marija and Radović, Svetlana and Ninković, Slavica",
year = "2018",
abstract = "Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataša seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.",
journal = "Protoplasma",
title = "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.",
doi = "10.1007/s00709-018-1250-0"
}

Banjac, N., Stanišić, M., Savić, J., Ćosić, T., Stanisavljević, N., Miljuš-Đukić, J., Marin, M., Radović, S.,& Ninković, S.. (2018). Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.. in Protoplasma.
https://doi.org/10.1007/s00709-018-1250-0

Banjac N, Stanišić M, Savić J, Ćosić T, Stanisavljević N, Miljuš-Đukić J, Marin M, Radović S, Ninković S. Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.. in Protoplasma. 2018;.
doi:10.1007/s00709-018-1250-0 .

Banjac, Nevena, Stanišić, Mariana, Savić, Jelena, Ćosić, Tatjana, Stanisavljević, Nemanja, Miljuš-Đukić, Jovanka, Marin, Marija, Radović, Svetlana, Ninković, Slavica, "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate." in Protoplasma (2018),
https://doi.org/10.1007/s00709-018-1250-0 . .

TY  - JOUR
AU  - Vinterhalter, Dragan
AU  - Savić, Jelena
AU  - Stanišić, Mariana
AU  - Jovanović, Ž
AU  - Vinterhalter, Branka
PY  - 2016
UR  - https://link.springer.com/article/10.1007%2Fs10265-016-0821-4#Notes
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2983
AB  - Phototropic (PT) and gravitropic (GT) bending are the two major tropic movements that determine the spatial position of potato shoots. We studied PT bending of potato plantlets grown under long-day photoperiods in several prearranged position setups providing different interactions with the GT response. Starting with the standard PT stimulation setup composed of unilateral irradiation of vertically positioned shoots, experiments were also done in antagonistic and synergistic setups and in treatments with horizontal displacement of the light source. In the standard setup, PT bending suppressed the GT bending, which could occur only if the PT stimulation was cancelled. The antagonistic position, with phototropism and gravitropism attempting to bend shoots in opposite directions, showed phototropism and gravitropism as independent bending events with the outcome varying throughout the day reflecting diurnal changes in the competence of individual tropic components. Whilst gravitropism was constant, phototropism had a marked daily fluctuation of its magnitude with a prominent morning maximum starting an hour after the dawn in the growth room and lasting for the next 6 h. When phototropism and gravitropism were aligned in a synergistic position, stimulating shoot bending in the same direction, there was little quantitative addition of their individual effects. The long period of morning PT bending maximum enabled multiple PT bending events to be conducted in succession, each one preceded by a separate lag phase. Studies of secondary PT events showed that potato plantlets can follow and adjust their shoot position in response to both vertical and horizontal movements of a light source. PT bending was reversible, since the 180° horizontal change of a blue light (BL) source position resulted in reversal of bending direction after a 20-min-long lag phase.
PB  - Springer-Verlag Tokyo
T2  - Journal of plant research
T1  - Interaction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots
DO  - 10.1007/s10265-016-0821-4
SP  - 1
EP  - 12
ER  - 

@article{
author = "Vinterhalter, Dragan and Savić, Jelena and Stanišić, Mariana and Jovanović, Ž and Vinterhalter, Branka",
year = "2016",
abstract = "Phototropic (PT) and gravitropic (GT) bending are the two major tropic movements that determine the spatial position of potato shoots. We studied PT bending of potato plantlets grown under long-day photoperiods in several prearranged position setups providing different interactions with the GT response. Starting with the standard PT stimulation setup composed of unilateral irradiation of vertically positioned shoots, experiments were also done in antagonistic and synergistic setups and in treatments with horizontal displacement of the light source. In the standard setup, PT bending suppressed the GT bending, which could occur only if the PT stimulation was cancelled. The antagonistic position, with phototropism and gravitropism attempting to bend shoots in opposite directions, showed phototropism and gravitropism as independent bending events with the outcome varying throughout the day reflecting diurnal changes in the competence of individual tropic components. Whilst gravitropism was constant, phototropism had a marked daily fluctuation of its magnitude with a prominent morning maximum starting an hour after the dawn in the growth room and lasting for the next 6 h. When phototropism and gravitropism were aligned in a synergistic position, stimulating shoot bending in the same direction, there was little quantitative addition of their individual effects. The long period of morning PT bending maximum enabled multiple PT bending events to be conducted in succession, each one preceded by a separate lag phase. Studies of secondary PT events showed that potato plantlets can follow and adjust their shoot position in response to both vertical and horizontal movements of a light source. PT bending was reversible, since the 180° horizontal change of a blue light (BL) source position resulted in reversal of bending direction after a 20-min-long lag phase.",
publisher = "Springer-Verlag Tokyo",
journal = "Journal of plant research",
title = "Interaction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots",
doi = "10.1007/s10265-016-0821-4",
pages = "1-12"
}

Vinterhalter, D., Savić, J., Stanišić, M., Jovanović, Ž.,& Vinterhalter, B.. (2016). Interaction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots. in Journal of plant research
Springer-Verlag Tokyo., 1-12.
https://doi.org/10.1007/s10265-016-0821-4

Vinterhalter D, Savić J, Stanišić M, Jovanović Ž, Vinterhalter B. Interaction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots. in Journal of plant research. 2016;:1-12.
doi:10.1007/s10265-016-0821-4 .

Vinterhalter, Dragan, Savić, Jelena, Stanišić, Mariana, Jovanović, Ž, Vinterhalter, Branka, "Interaction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots" in Journal of plant research (2016):1-12,
https://doi.org/10.1007/s10265-016-0821-4 . .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Banjac, Nevena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4998
AB  - The effect of antibiotics hygromycin, cefotaxime and meropenem on shoot regeneration in apple cultivar “Golden Delicious” was evaluated to optimize the protocol for Agrobacterium tumefaciens-mediated genetic transformation. The careful determination of antibiotics concentration is important step to ensure efficient elimination of Agrobacterium and appropriate selection process without escapes and detrimental effect on plant regeneration. Healthy and fully expanded young leaves of in vitro grown apple shoots were cut
transversely into three pieces that were used as explants for shoot regeneration. Regeneration frequency, the
mean number of shoots per explant and the index of shoot-forming capacity were calculated after 8 weeks
of culturing on Murashige and Skoog medium containing 5 mg L-1 thidiazuron (TDZ) and 0.3 mg L-1 indole-
3-butyric acid (IBA), supplemented with hygromycin (0, 2, 4, 5, 6, 8 and 10 mg L-1), cefotaxime (0, 100, 200,
300, 400, 500 and 600 mg L-1) or meropenem (0, 25, 50, 75, 100, 125 and 150 mg L-1). Results indicated that
the highest index of shoot-forming capacity was obtained at 300 mg L-1 cefotaxime (3.67) comparing to control (2.93) and other antibiotic treatments. The increase of hygromycin concentration significantly decreased
regeneration efficiency, while at concentrations of 5 mg L-1 and higher it completely inhibited shoot regeneration. Therefore, the present study outlines the use of cefotaxime at 300 mg L-1 and a stepwise selection procedure with the final hygromycin concentration of 5 mg L-1 as a suitable system for A. tumefaciens-mediated
genetic transformation of apple cultivar “Golden Delicious”.
PB  - Belgrade : Serbian Plant Physiology Society : Institute for Biological Research “Siniša Stanković”
C3  - 2nd International Conference on Plant Biology; 21th Symposium of the Serbian Plant Physiology Society; COST ACTION FA1106 QUALITYFRUIT Workshop
T1  - The effect of antibiotics on the shoot regeneration in apple cultivar “Golden Delicious”
SP  - 28
EP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4998
ER  - 

@conference{
author = "Stanišić, Mariana and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Banjac, Nevena",
year = "2015",
abstract = "The effect of antibiotics hygromycin, cefotaxime and meropenem on shoot regeneration in apple cultivar “Golden Delicious” was evaluated to optimize the protocol for Agrobacterium tumefaciens-mediated genetic transformation. The careful determination of antibiotics concentration is important step to ensure efficient elimination of Agrobacterium and appropriate selection process without escapes and detrimental effect on plant regeneration. Healthy and fully expanded young leaves of in vitro grown apple shoots were cut
transversely into three pieces that were used as explants for shoot regeneration. Regeneration frequency, the
mean number of shoots per explant and the index of shoot-forming capacity were calculated after 8 weeks
of culturing on Murashige and Skoog medium containing 5 mg L-1 thidiazuron (TDZ) and 0.3 mg L-1 indole-
3-butyric acid (IBA), supplemented with hygromycin (0, 2, 4, 5, 6, 8 and 10 mg L-1), cefotaxime (0, 100, 200,
300, 400, 500 and 600 mg L-1) or meropenem (0, 25, 50, 75, 100, 125 and 150 mg L-1). Results indicated that
the highest index of shoot-forming capacity was obtained at 300 mg L-1 cefotaxime (3.67) comparing to control (2.93) and other antibiotic treatments. The increase of hygromycin concentration significantly decreased
regeneration efficiency, while at concentrations of 5 mg L-1 and higher it completely inhibited shoot regeneration. Therefore, the present study outlines the use of cefotaxime at 300 mg L-1 and a stepwise selection procedure with the final hygromycin concentration of 5 mg L-1 as a suitable system for A. tumefaciens-mediated
genetic transformation of apple cultivar “Golden Delicious”.",
publisher = "Belgrade : Serbian Plant Physiology Society : Institute for Biological Research “Siniša Stanković”",
journal = "2nd International Conference on Plant Biology; 21th Symposium of the Serbian Plant Physiology Society; COST ACTION FA1106 QUALITYFRUIT Workshop",
title = "The effect of antibiotics on the shoot regeneration in apple cultivar “Golden Delicious”",
pages = "28-29",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4998"
}

Stanišić, M., Ninković, S., Savić, J., Ćosić, T.,& Banjac, N.. (2015). The effect of antibiotics on the shoot regeneration in apple cultivar “Golden Delicious”. in 2nd International Conference on Plant Biology; 21th Symposium of the Serbian Plant Physiology Society; COST ACTION FA1106 QUALITYFRUIT Workshop
Belgrade : Serbian Plant Physiology Society : Institute for Biological Research “Siniša Stanković”., 28-29.
https://hdl.handle.net/21.15107/rcub_ibiss_4998

Stanišić M, Ninković S, Savić J, Ćosić T, Banjac N. The effect of antibiotics on the shoot regeneration in apple cultivar “Golden Delicious”. in 2nd International Conference on Plant Biology; 21th Symposium of the Serbian Plant Physiology Society; COST ACTION FA1106 QUALITYFRUIT Workshop. 2015;:28-29.
https://hdl.handle.net/21.15107/rcub_ibiss_4998 .

Stanišić, Mariana, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Banjac, Nevena, "The effect of antibiotics on the shoot regeneration in apple cultivar “Golden Delicious”" in 2nd International Conference on Plant Biology; 21th Symposium of the Serbian Plant Physiology Society; COST ACTION FA1106 QUALITYFRUIT Workshop (2015):28-29,
https://hdl.handle.net/21.15107/rcub_ibiss_4998 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Savić, Jelena
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3793
AB  - Goosefoot (Chenopodium murale L.) is a cosmopolitan weed species whose root produces substances
with allelopathic effects to various plant species, including agricultural ones. To investigate the
allelopathic effect of C. murale on vegetable plant collard greens (Brassica oleracea L. var. acephala),
the sterilized seeds of collard greens were treated with liquid nutrient medium in which C. murale
hairy root clone R5 was grown for 4 weeks and which contained its exudates. Results indicated that
final germination rate was not affected by R5 while the growth and development of collard greens
seedlings were significantly inhibited. The roots were more affected than shoots. Comparing to the
control, seedlings treated with R5 had reduced number of roots per seedling (27%), root length
(33%) and root fresh weight (59%). Further, R5 led to the seedlings bending, loss of chlorophyll in
the leaves, root necrosis and finally seedlings decay, indicating the ultimate lethal effect of C. murale
allelochemicals. The seedling growth inhibition was accompanied with alteration in antioxidant
enzymes activities illustrated by increased peroxidase (POD), superoxide dismutase (SOD) and
catalase (CAT) activity in shoots and emphasized CAT and decreased SOD activity in roots. The
results could be contributed to the dissemination of knowledge about allelopathic influence of C.
murale on cultivated plants and can be helpful in determining the mode of action against this weed
species to protect collard greens plants
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - Chenopodium murale hairy root exudates inhibit growth and induce oxidative stress in collard greens (Brassica oleracea L. var. acephala)
IS  - 2
VL  - 39
SP  - 187
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3793
ER  - 

@article{
author = "Banjac, Nevena and Stanišić, Mariana and Savić, Jelena and Zdravković-Korać, Snežana and Ninković, Slavica",
year = "2015",
abstract = "Goosefoot (Chenopodium murale L.) is a cosmopolitan weed species whose root produces substances
with allelopathic effects to various plant species, including agricultural ones. To investigate the
allelopathic effect of C. murale on vegetable plant collard greens (Brassica oleracea L. var. acephala),
the sterilized seeds of collard greens were treated with liquid nutrient medium in which C. murale
hairy root clone R5 was grown for 4 weeks and which contained its exudates. Results indicated that
final germination rate was not affected by R5 while the growth and development of collard greens
seedlings were significantly inhibited. The roots were more affected than shoots. Comparing to the
control, seedlings treated with R5 had reduced number of roots per seedling (27%), root length
(33%) and root fresh weight (59%). Further, R5 led to the seedlings bending, loss of chlorophyll in
the leaves, root necrosis and finally seedlings decay, indicating the ultimate lethal effect of C. murale
allelochemicals. The seedling growth inhibition was accompanied with alteration in antioxidant
enzymes activities illustrated by increased peroxidase (POD), superoxide dismutase (SOD) and
catalase (CAT) activity in shoots and emphasized CAT and decreased SOD activity in roots. The
results could be contributed to the dissemination of knowledge about allelopathic influence of C.
murale on cultivated plants and can be helpful in determining the mode of action against this weed
species to protect collard greens plants",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "Chenopodium murale hairy root exudates inhibit growth and induce oxidative stress in collard greens (Brassica oleracea L. var. acephala)",
number = "2",
volume = "39",
pages = "187-195",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3793"
}

Banjac, N., Stanišić, M., Savić, J., Zdravković-Korać, S.,& Ninković, S.. (2015). Chenopodium murale hairy root exudates inhibit growth and induce oxidative stress in collard greens (Brassica oleracea L. var. acephala). in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 39(2), 187-195.
https://hdl.handle.net/21.15107/rcub_ibiss_3793

Banjac N, Stanišić M, Savić J, Zdravković-Korać S, Ninković S. Chenopodium murale hairy root exudates inhibit growth and induce oxidative stress in collard greens (Brassica oleracea L. var. acephala). in Botanica Serbica. 2015;39(2):187-195.
https://hdl.handle.net/21.15107/rcub_ibiss_3793 .

Banjac, Nevena, Stanišić, Mariana, Savić, Jelena, Zdravković-Korać, Snežana, Ninković, Slavica, "Chenopodium murale hairy root exudates inhibit growth and induce oxidative stress in collard greens (Brassica oleracea L. var. acephala)" in Botanica Serbica, 39, no. 2 (2015):187-195,
https://hdl.handle.net/21.15107/rcub_ibiss_3793 .

TY  - JOUR
AU  - Stanišić, Mariana
AU  - Raspor, Martin
AU  - Ninković, Slavica
AU  - Milošević, Snežana
AU  - Ćalić, Dušica
AU  - Bohanec, Borut
AU  - Trifunović-Momčilov, Milana
AU  - Petrić, Marija
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2094
UR  - https://s100.copyright.com/AppDispatchServlet?publisherName=ELS&contentID=S025462991400194X&orderBeanReset=true
AB  - Efficient protocols, safe from somaclonal variation, were developed for
   regeneration of Iris sibirica plants via organogenesis and somatic
   embryogenesis from leaf-base explants cultivated on Murashige and Skoog
   media supplemented with thidiazuron (TDZ, 1.0 mg/l) or
   2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/l). The morphogenic
   response and callus formation efficiency differed significantly between
   2,4-D (80.9\%) and TDZ (67\%) morphogenesis induction treatments. TDZ
   induced only organogenic calli, while calli obtained with 2,4-D were
   composed of three types differing in color and consistency: white,
   friable - embryogenic calli (4.5\%, 3.8 mg/explant), green, compact -
   organogenic calli (12.4\%, 48.4 mg/explants) and yellow -
   non-regenerative calli (773\%, 254.4 mg/explant). The cultivation of
   embryogenic calli on medium with 2,4-0 and Kinetin resulted in further
   development of somatic embryos (54 embryos/g of calli) which germinated
   with a frequency of 62\% after being transferred to a medium without
   plant growth regulators. Stable shoot cultures were established by
   transferring organogenic calli with shoot primordia to media with 0.1
   mg/l alpha-naphthaleneacetic acid (NAA) and 1.0 mg/l 6-benzylaminopurine
   (BA), while further cultivation on media of the same composition (TDZ or
   2,4-D) resulted in the reduced growth and rhizogenesis, respectively.
   The TDZ induction treatment resulted in higher number of shoots per
   explant (7.9) than the 2,4-0 treatment (43). After successful rooting
   and ex vitro acclimatization, plants were grown in the field and
   flowered to seed production. Flow cytometry, chromosome counting and
   random amplification of polymorphic DNA (RAPD) analysis indicated no
   evidence of genetic variation in plants regenerated via somatic
   embryogenesis or organogenesis. The results suggest that established
   protocols are safe for use in genetic transformation procedures or
   large-scale production of true-to-type I. sibirica plants. (C) 2014
   SAAB. Published by Elsevier B.V. All rights reserved.
T2  - South African Journal of Botany
T1  - Clonal fidelity of Iris sibirica plants regenerated by somatic
 embryogenesis and organogenesis in leaf-base culture - RAPD and flow
 cytometer analyses
VL  - 96
DO  - 10.1016/j.sajb.2014.10.014
SP  - 42
EP  - 52
ER  - 

@article{
author = "Stanišić, Mariana and Raspor, Martin and Ninković, Slavica and Milošević, Snežana and Ćalić, Dušica and Bohanec, Borut and Trifunović-Momčilov, Milana and Petrić, Marija and Subotić, Angelina and Jevremović, Slađana",
year = "2015",
abstract = "Efficient protocols, safe from somaclonal variation, were developed for
   regeneration of Iris sibirica plants via organogenesis and somatic
   embryogenesis from leaf-base explants cultivated on Murashige and Skoog
   media supplemented with thidiazuron (TDZ, 1.0 mg/l) or
   2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/l). The morphogenic
   response and callus formation efficiency differed significantly between
   2,4-D (80.9\%) and TDZ (67\%) morphogenesis induction treatments. TDZ
   induced only organogenic calli, while calli obtained with 2,4-D were
   composed of three types differing in color and consistency: white,
   friable - embryogenic calli (4.5\%, 3.8 mg/explant), green, compact -
   organogenic calli (12.4\%, 48.4 mg/explants) and yellow -
   non-regenerative calli (773\%, 254.4 mg/explant). The cultivation of
   embryogenic calli on medium with 2,4-0 and Kinetin resulted in further
   development of somatic embryos (54 embryos/g of calli) which germinated
   with a frequency of 62\% after being transferred to a medium without
   plant growth regulators. Stable shoot cultures were established by
   transferring organogenic calli with shoot primordia to media with 0.1
   mg/l alpha-naphthaleneacetic acid (NAA) and 1.0 mg/l 6-benzylaminopurine
   (BA), while further cultivation on media of the same composition (TDZ or
   2,4-D) resulted in the reduced growth and rhizogenesis, respectively.
   The TDZ induction treatment resulted in higher number of shoots per
   explant (7.9) than the 2,4-0 treatment (43). After successful rooting
   and ex vitro acclimatization, plants were grown in the field and
   flowered to seed production. Flow cytometry, chromosome counting and
   random amplification of polymorphic DNA (RAPD) analysis indicated no
   evidence of genetic variation in plants regenerated via somatic
   embryogenesis or organogenesis. The results suggest that established
   protocols are safe for use in genetic transformation procedures or
   large-scale production of true-to-type I. sibirica plants. (C) 2014
   SAAB. Published by Elsevier B.V. All rights reserved.",
journal = "South African Journal of Botany",
title = "Clonal fidelity of Iris sibirica plants regenerated by somatic
 embryogenesis and organogenesis in leaf-base culture - RAPD and flow
 cytometer analyses",
volume = "96",
doi = "10.1016/j.sajb.2014.10.014",
pages = "42-52"
}

Stanišić, M., Raspor, M., Ninković, S., Milošević, S., Ćalić, D., Bohanec, B., Trifunović-Momčilov, M., Petrić, M., Subotić, A.,& Jevremović, S.. (2015). Clonal fidelity of Iris sibirica plants regenerated by somatic
 embryogenesis and organogenesis in leaf-base culture - RAPD and flow
 cytometer analyses. in South African Journal of Botany, 96, 42-52.
https://doi.org/10.1016/j.sajb.2014.10.014

Stanišić M, Raspor M, Ninković S, Milošević S, Ćalić D, Bohanec B, Trifunović-Momčilov M, Petrić M, Subotić A, Jevremović S. Clonal fidelity of Iris sibirica plants regenerated by somatic
 embryogenesis and organogenesis in leaf-base culture - RAPD and flow
 cytometer analyses. in South African Journal of Botany. 2015;96:42-52.
doi:10.1016/j.sajb.2014.10.014 .

Stanišić, Mariana, Raspor, Martin, Ninković, Slavica, Milošević, Snežana, Ćalić, Dušica, Bohanec, Borut, Trifunović-Momčilov, Milana, Petrić, Marija, Subotić, Angelina, Jevremović, Slađana, "Clonal fidelity of Iris sibirica plants regenerated by somatic
 embryogenesis and organogenesis in leaf-base culture - RAPD and flow
 cytometer analyses" in South African Journal of Botany, 96 (2015):42-52,
https://doi.org/10.1016/j.sajb.2014.10.014 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Dmitrović, Slavica
AU  - Đorđević, Mirka
AU  - Zdravković-Korać, Snežana
AU  - Nikolić, Radomirka R
AU  - Raspor, Martin
AU  - Ćosić, Tatjana
AU  - Maksimović, Vuk M
AU  - Živković, Suzana
AU  - Krstić Milošević, Dijana
AU  - Stanišić, Mariana
AU  - Ninković, Slavica
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1150
AB  - We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. (C) 2012 Elsevier GmbH. All rights reserved.
T2  - Journal of Plant Physiology
T1  - Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
IS  - 12
VL  - 169
DO  - 10.1016/j.jplph.2012.04.009
SP  - 323
EP  - 1211
ER  - 

@article{
author = "Banjac, Nevena and Dmitrović, Slavica and Đorđević, Mirka and Zdravković-Korać, Snežana and Nikolić, Radomirka R and Raspor, Martin and Ćosić, Tatjana and Maksimović, Vuk M and Živković, Suzana and Krstić Milošević, Dijana and Stanišić, Mariana and Ninković, Slavica",
year = "2012",
abstract = "We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. (C) 2012 Elsevier GmbH. All rights reserved.",
journal = "Journal of Plant Physiology",
title = "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays",
number = "12",
volume = "169",
doi = "10.1016/j.jplph.2012.04.009",
pages = "323-1211"
}

Banjac, N., Dmitrović, S., Đorđević, M., Zdravković-Korać, S., Nikolić, R. R., Raspor, M., Ćosić, T., Maksimović, V. M., Živković, S., Krstić Milošević, D., Stanišić, M.,& Ninković, S.. (2012). Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology, 169(12), 323-1211.
https://doi.org/10.1016/j.jplph.2012.04.009

Banjac N, Dmitrović S, Đorđević M, Zdravković-Korać S, Nikolić RR, Raspor M, Ćosić T, Maksimović VM, Živković S, Krstić Milošević D, Stanišić M, Ninković S. Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology. 2012;169(12):323-1211.
doi:10.1016/j.jplph.2012.04.009 .

Banjac, Nevena, Dmitrović, Slavica, Đorđević, Mirka, Zdravković-Korać, Snežana, Nikolić, Radomirka R, Raspor, Martin, Ćosić, Tatjana, Maksimović, Vuk M, Živković, Suzana, Krstić Milošević, Dijana, Stanišić, Mariana, Ninković, Slavica, "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays" in Journal of Plant Physiology, 169, no. 12 (2012):323-1211,
https://doi.org/10.1016/j.jplph.2012.04.009 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana B
AU  - Ćosić, Tatjana
AU  - Nikolić, Radomirka R
AU  - Ninković, Slavica
AU  - Miletić, Rade K
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1152
AB  - An efficient in vitro shoot regeneration method from leaf explants of apple cultivars Golden Delicious and Melrose by optimization of regeneration medium, explant type and orientation, dark pre-treatment, and gelling agent is presented. Murashige and Skoog's regeneration medium containing 22 mu M thidiazuron (TDZ) and 1.5 mu M indole-3-butyric acid (IBA) (M2 medium) was superior for regeneration as well as for subsequent shoot multiplication in both cultivars, providing regeneration frequency of 95% or higher in the best combination with other factors. Pre-incubation in the dark proved to be an essential factor for regeneration. The use of agar as a gelling agent provides satisfactory regeneration frequency compared with media gelled with Phyta-gel (TM). Leaf explants of cv. Melrose with adaxial surface in contact with M2 medium and those of cv. Golden Delicious orientated contrary regenerated the highest mean number of shoots per explant. Under optimal conditions, a maximal index of shoot-forming capacity of 11.44 and 6.30 for 'Melrose' and 'Golden Delicious', respectively, was achieved. Regenerated shoots were successfully rooted and acclimated ex vitro.
T2  - Hortscience
T1  - Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose
IS  - 8
VL  - 47
DO  - 10.21273/HORTSCI.47.8.1117
SP  - 1337
EP  - 1122
ER  - 

@article{
author = "Banjac, Nevena and Stanišić, Mariana and Milojević, Jelena and Tubić, Ljiljana B and Ćosić, Tatjana and Nikolić, Radomirka R and Ninković, Slavica and Miletić, Rade K",
year = "2012",
abstract = "An efficient in vitro shoot regeneration method from leaf explants of apple cultivars Golden Delicious and Melrose by optimization of regeneration medium, explant type and orientation, dark pre-treatment, and gelling agent is presented. Murashige and Skoog's regeneration medium containing 22 mu M thidiazuron (TDZ) and 1.5 mu M indole-3-butyric acid (IBA) (M2 medium) was superior for regeneration as well as for subsequent shoot multiplication in both cultivars, providing regeneration frequency of 95% or higher in the best combination with other factors. Pre-incubation in the dark proved to be an essential factor for regeneration. The use of agar as a gelling agent provides satisfactory regeneration frequency compared with media gelled with Phyta-gel (TM). Leaf explants of cv. Melrose with adaxial surface in contact with M2 medium and those of cv. Golden Delicious orientated contrary regenerated the highest mean number of shoots per explant. Under optimal conditions, a maximal index of shoot-forming capacity of 11.44 and 6.30 for 'Melrose' and 'Golden Delicious', respectively, was achieved. Regenerated shoots were successfully rooted and acclimated ex vitro.",
journal = "Hortscience",
title = "Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose",
number = "8",
volume = "47",
doi = "10.21273/HORTSCI.47.8.1117",
pages = "1337-1122"
}

Banjac, N., Stanišić, M., Milojević, J., Tubić, L. B., Ćosić, T., Nikolić, R. R., Ninković, S.,& Miletić, R. K.. (2012). Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose. in Hortscience, 47(8), 1337-1122.
https://doi.org/10.21273/HORTSCI.47.8.1117

Banjac N, Stanišić M, Milojević J, Tubić LB, Ćosić T, Nikolić RR, Ninković S, Miletić RK. Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose. in Hortscience. 2012;47(8):1337-1122.
doi:10.21273/HORTSCI.47.8.1117 .

Banjac, Nevena, Stanišić, Mariana, Milojević, Jelena, Tubić, Ljiljana B, Ćosić, Tatjana, Nikolić, Radomirka R, Ninković, Slavica, Miletić, Rade K, "Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose" in Hortscience, 47, no. 8 (2012):1337-1122,
https://doi.org/10.21273/HORTSCI.47.8.1117 . .