TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6555
AB  - Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.
PB  - Elsevier Ltd.
T2  - Medical Hypotheses
T1  - Can food affect the perception of time?
VL  - 184
DO  - 10.1016/j.mehy.2024.111283
SP  - 111283
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka and Filipović, Branko",
year = "2024",
abstract = "Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.",
publisher = "Elsevier Ltd.",
journal = "Medical Hypotheses",
title = "Can food affect the perception of time?",
volume = "184",
doi = "10.1016/j.mehy.2024.111283",
pages = "111283"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B.,& Filipović, B.. (2024). Can food affect the perception of time?. in Medical Hypotheses
Elsevier Ltd.., 184, 111283.
https://doi.org/10.1016/j.mehy.2024.111283

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B. Can food affect the perception of time?. in Medical Hypotheses. 2024;184:111283.
doi:10.1016/j.mehy.2024.111283 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka, Filipović, Branko, "Can food affect the perception of time?" in Medical Hypotheses, 184 (2024):111283,
https://doi.org/10.1016/j.mehy.2024.111283 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6554
AB  - Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.
PB  - Elsevier Ltd.
T2  - Medical Hypotheses
T1  - Can food affect the perception of time?
VL  - 184
DO  - 10.1016/j.mehy.2024.111283
SP  - 111283
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka and Filipović, Branko",
year = "2024",
abstract = "Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.",
publisher = "Elsevier Ltd.",
journal = "Medical Hypotheses",
title = "Can food affect the perception of time?",
volume = "184",
doi = "10.1016/j.mehy.2024.111283",
pages = "111283"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B.,& Filipović, B.. (2024). Can food affect the perception of time?. in Medical Hypotheses
Elsevier Ltd.., 184, 111283.
https://doi.org/10.1016/j.mehy.2024.111283

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B. Can food affect the perception of time?. in Medical Hypotheses. 2024;184:111283.
doi:10.1016/j.mehy.2024.111283 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka, Filipović, Branko, "Can food affect the perception of time?" in Medical Hypotheses, 184 (2024):111283,
https://doi.org/10.1016/j.mehy.2024.111283 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Milenkovic, Dragan
AU  - Cesar, Thais
AU  - Filipović R., Miloš
AU  - Milošević, Verica
PY  - 2024
UR  - https://iubmb.onlinelibrary.wiley.com/journal/18728081
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6462
AB  - Citrus flavanones are recognized as promising bioactives within the concept of healthy aging. Thus, the present study investigated the effects of a nutritionally relevant dose of lemon extract (LE) on liver redox regulation and persulfidation levels in 24-month-old Wistar rats. LE (40 mg/kg b.m.) was administered orally once daily for four weeks. Control groups received either vehicle (sunflower oil) or remained intact. The applied methodology considered qPCR, Western blot, protein persulfidation levels evaluation, histochemistry in line with immunofluorescence, liver biochemical assays (glutathione, total -SH groups and malonaldehyde; MDA), liver enzymes in serum and in silico analysis to explore the potential interaction/binding between the proteins studied in the paper. Our results showed that LE increased glutathione peroxidase (GPx), reductase (GR), glutamate-cysteine ligase catalytic and modifier subunit, respectively, as well as Nrf2 gene expressions, but decreased the expression of superoxide dismutase 2 (SOD2). Upon LE application, protein expression showed upregulation of NRF2, SOD2, GPx, GR, and thioredoxin 1 (Trx1). LE significantly decreased the protein persulfidation levels and concentration of MDA, a marker of oxidative damage in the cell. Histological analysis showed a normal liver histoarchitecture without pathological changes, aligning with the normal serum level of hepatic enzymes. Obtained results showed that LE, by modulating hepatic redox regulators Nrf2 and Trx1, diminishes oxidative stress and alters the persulfidation levels, suggesting a considerable beneficial antioxidant potential of lemon flavanones in the old-aged liver.
PB  - John Wiley and Sons
T2  - Biofactors
T1  - Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats
DO  - 10.1002/biof.2038
ER  - 

@article{
author = "Miler, Marko and Živanović, Jasmina and Ajdžanović, Vladimir and Milenkovic, Dragan and Cesar, Thais and Filipović R., Miloš and Milošević, Verica",
year = "2024",
abstract = "Citrus flavanones are recognized as promising bioactives within the concept of healthy aging. Thus, the present study investigated the effects of a nutritionally relevant dose of lemon extract (LE) on liver redox regulation and persulfidation levels in 24-month-old Wistar rats. LE (40 mg/kg b.m.) was administered orally once daily for four weeks. Control groups received either vehicle (sunflower oil) or remained intact. The applied methodology considered qPCR, Western blot, protein persulfidation levels evaluation, histochemistry in line with immunofluorescence, liver biochemical assays (glutathione, total -SH groups and malonaldehyde; MDA), liver enzymes in serum and in silico analysis to explore the potential interaction/binding between the proteins studied in the paper. Our results showed that LE increased glutathione peroxidase (GPx), reductase (GR), glutamate-cysteine ligase catalytic and modifier subunit, respectively, as well as Nrf2 gene expressions, but decreased the expression of superoxide dismutase 2 (SOD2). Upon LE application, protein expression showed upregulation of NRF2, SOD2, GPx, GR, and thioredoxin 1 (Trx1). LE significantly decreased the protein persulfidation levels and concentration of MDA, a marker of oxidative damage in the cell. Histological analysis showed a normal liver histoarchitecture without pathological changes, aligning with the normal serum level of hepatic enzymes. Obtained results showed that LE, by modulating hepatic redox regulators Nrf2 and Trx1, diminishes oxidative stress and alters the persulfidation levels, suggesting a considerable beneficial antioxidant potential of lemon flavanones in the old-aged liver.",
publisher = "John Wiley and Sons",
journal = "Biofactors",
title = "Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats",
doi = "10.1002/biof.2038"
}

Miler, M., Živanović, J., Ajdžanović, V., Milenkovic, D., Cesar, T., Filipović R., M.,& Milošević, V.. (2024). Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats. in Biofactors
John Wiley and Sons..
https://doi.org/10.1002/biof.2038

Miler M, Živanović J, Ajdžanović V, Milenkovic D, Cesar T, Filipović R. M, Milošević V. Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats. in Biofactors. 2024;.
doi:10.1002/biof.2038 .

Miler, Marko, Živanović, Jasmina, Ajdžanović, Vladimir, Milenkovic, Dragan, Cesar, Thais, Filipović R., Miloš, Milošević, Verica, "Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats" in Biofactors (2024),
https://doi.org/10.1002/biof.2038 . .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Čukuranović-Kokoris, Jovana
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6405
AB  - Uvod. Aplikacija daidzeina može predstavljati efikasnu i manje rizičnu altemativu indikovanoj, klasičnoj estrogenizaciji starijih muškaraca. Cilj ove studije je podrazumevao morfofiziološku analizu kore nadbubrežne žlezde u pacovskom modelu uznapredovale andropauze, nakon primene daidzeina.
Materijal i metode. Sredovečni (16 meseci stari) pacovi Wistar soja su razdeljeni u
lažno operisanu (SO; n=8), orhidektomisanu (Orx; n=8) i daidzeinom tretiranu orhidektomisanu (Orx+D; n=8) grupu. Daidzein (30 mg/kg t.m./dan) je subkutano davan tri nedelje, dok su SO i Orx grupa dobijale samo rastvarač tokom istog perioda. Ciljevi su realizovani korišćenjem stereologije, histohemije/imunohistohemije, imunoeseja i ultrastruktume analize.
Rezultati. Tretman daidzeinom je značajno uvećao volumene ćelija i jedara zone glomeruloze 
za 32% i 41% tim redom, ali je i snizio (p<0.05) nivo cirkulišućeg aldosterona za 61%, sve u 
poređenju sa odgovarajućim parametrima kod Orx zivotinja. Takođe, daidzein je značajno smanjio 
volumen jedara ćelija zone fascikulate, kao i tkivni i cirkulišući nivo kortikosterona, za 24%, 48% i 33% 
navedenim redom, te izrazito (p<0.05) povisio cirkulišući nivo DHEA za oko 4.6 puta, sve u 
poređenju sa odgovarajućim parametrima kod Orx pacova. Daidzeinom prouzrokovano preusmeravanje 
puteva kortikosteroidogeneze ka produkciji adrenalnih androgena bilo je u skladu sa ultrastruktumim nalazom i rearanžmanima kapilarne rnreže u okviru konkretnih zona kore nadbubrežne zlezde.
Zaključak. Imajući u vidu sklad efekata daidzeina u ispitivanom kontekstu, kao relativnu bezbednost njegove upotrebe, moglo bi se reći da daidzein ima perspektivu u tretrnanu starenjem uzorokovanog gubitka androgena, te hiperfunkcije hipotalamo-hipofizno-adrenalne ose i povezanih metaboličkih poremećaja kod muškaraca.
AB  - Introduction. Daidzein application may represent effective and less harmful alternative to indicated, classical estrogenization of the aging men. The aim of this study was to perform morphophysiological analysis of the adrenal cortex, after daidzein supplementation in a rat model of the severe andropause.
Material and methods. Middle-aged (16 months old) Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and daidzein treated orchidectomized (Orx+D; n=8) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis.
Results. Daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei by 32% and 41% respectively, but decreased (p<0.05) circulating aldosterone levels by 61%, all in comparison with the adequate parameters in Orx animals. Also, daidzein significantly decreased zona fasciculata cell nuclei volume, as well as both the adrenal and circulating levels of corticosterone, by 24%, 48% and 33% respectively, but strongly (p<0.05) increased the circulating level of DHEA by about 4.6 times, all when compared to the corresponding parameters of Orx rats. Daidzein-induced shunting of the corticosteroidogenesis pathways towards adrenal androgens production corresponded to the ultrastructural findings and zonal capillary network rearrangements.
Conclusion. Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of aging-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.
PB  - Niš: University of Niš, Faculty of Medicine
C3  - Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
T1  - Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze
T1  - Morphophysiology of the adrenal cortex after daidzein application in a rat model of the severe andropause
SP  - 166
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6405
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Čukuranović-Kokoris, Jovana and Miler, Marko and Šošić-Jurjević, Branka and Živanović, Jasmina and Marković Filipović, Jelena and Milošević, Verica and Filipović, Branko",
year = "2023",
abstract = "Uvod. Aplikacija daidzeina može predstavljati efikasnu i manje rizičnu altemativu indikovanoj, klasičnoj estrogenizaciji starijih muškaraca. Cilj ove studije je podrazumevao morfofiziološku analizu kore nadbubrežne žlezde u pacovskom modelu uznapredovale andropauze, nakon primene daidzeina.
Materijal i metode. Sredovečni (16 meseci stari) pacovi Wistar soja su razdeljeni u
lažno operisanu (SO; n=8), orhidektomisanu (Orx; n=8) i daidzeinom tretiranu orhidektomisanu (Orx+D; n=8) grupu. Daidzein (30 mg/kg t.m./dan) je subkutano davan tri nedelje, dok su SO i Orx grupa dobijale samo rastvarač tokom istog perioda. Ciljevi su realizovani korišćenjem stereologije, histohemije/imunohistohemije, imunoeseja i ultrastruktume analize.
Rezultati. Tretman daidzeinom je značajno uvećao volumene ćelija i jedara zone glomeruloze 
za 32% i 41% tim redom, ali je i snizio (p<0.05) nivo cirkulišućeg aldosterona za 61%, sve u 
poređenju sa odgovarajućim parametrima kod Orx zivotinja. Takođe, daidzein je značajno smanjio 
volumen jedara ćelija zone fascikulate, kao i tkivni i cirkulišući nivo kortikosterona, za 24%, 48% i 33% 
navedenim redom, te izrazito (p<0.05) povisio cirkulišući nivo DHEA za oko 4.6 puta, sve u 
poređenju sa odgovarajućim parametrima kod Orx pacova. Daidzeinom prouzrokovano preusmeravanje 
puteva kortikosteroidogeneze ka produkciji adrenalnih androgena bilo je u skladu sa ultrastruktumim nalazom i rearanžmanima kapilarne rnreže u okviru konkretnih zona kore nadbubrežne zlezde.
Zaključak. Imajući u vidu sklad efekata daidzeina u ispitivanom kontekstu, kao relativnu bezbednost njegove upotrebe, moglo bi se reći da daidzein ima perspektivu u tretrnanu starenjem uzorokovanog gubitka androgena, te hiperfunkcije hipotalamo-hipofizno-adrenalne ose i povezanih metaboličkih poremećaja kod muškaraca., Introduction. Daidzein application may represent effective and less harmful alternative to indicated, classical estrogenization of the aging men. The aim of this study was to perform morphophysiological analysis of the adrenal cortex, after daidzein supplementation in a rat model of the severe andropause.
Material and methods. Middle-aged (16 months old) Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and daidzein treated orchidectomized (Orx+D; n=8) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis.
Results. Daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei by 32% and 41% respectively, but decreased (p<0.05) circulating aldosterone levels by 61%, all in comparison with the adequate parameters in Orx animals. Also, daidzein significantly decreased zona fasciculata cell nuclei volume, as well as both the adrenal and circulating levels of corticosterone, by 24%, 48% and 33% respectively, but strongly (p<0.05) increased the circulating level of DHEA by about 4.6 times, all when compared to the corresponding parameters of Orx rats. Daidzein-induced shunting of the corticosteroidogenesis pathways towards adrenal androgens production corresponded to the ultrastructural findings and zonal capillary network rearrangements.
Conclusion. Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of aging-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.",
publisher = "Niš: University of Niš, Faculty of Medicine",
journal = "Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia",
title = "Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze, Morphophysiology of the adrenal cortex after daidzein application in a rat model of the severe andropause",
pages = "166-167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6405"
}

Ajdžanović, V., Čukuranović-Kokoris, J., Miler, M., Šošić-Jurjević, B., Živanović, J., Marković Filipović, J., Milošević, V.,& Filipović, B.. (2023). Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
Niš: University of Niš, Faculty of Medicine., 166-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6405

Ajdžanović V, Čukuranović-Kokoris J, Miler M, Šošić-Jurjević B, Živanović J, Marković Filipović J, Milošević V, Filipović B. Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia. 2023;:166-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6405 .

Ajdžanović, Vladimir, Čukuranović-Kokoris, Jovana, Miler, Marko, Šošić-Jurjević, Branka, Živanović, Jasmina, Marković Filipović, Jelena, Milošević, Verica, Filipović, Branko, "Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze" in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia (2023):166-167,
https://hdl.handle.net/21.15107/rcub_ibiss_6405 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6279
AB  - Prostate health is related to the frequency of ejaculation and prostate drainage. Frequent, repetitive ejaculations theoretically may pose a prostate cancer risk due to ensuing high testosterone levels and loss of zinc. In line with this, the proximity of the human rectum and prostate is of diagnostic and therapeutic importance. We provide evidence to support the view that the proximity of the rectum and prostate in men enables lower bowel distention to compress the prostate's lateral side to facilitate drainage, which might lower cancer risk. This drainage mechanism is likely on-going and gradual, compared to rapid drainage following ejaculation. The physiological context suggests that these mechanisms of prostate drainage are complementary. Dietary content containing polyphenol- and fiber-rich meals, along with voluminous stool formation and increased gastrointestinal transit may all help improve prostate drainage. This may be important in voluntary abstinent men or other categories of sexually limited men. Recognizing that the anatomic relationship between the rectal area and prostate may facilitate prostate drainage, because of diet and gastro-intestinal activity, could be important to prostate health maintenance.
PB  - MRE Press
T2  - Journal of Men's Health
T1  - The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health
IS  - 10
VL  - 19
DO  - 10.22514/jomh.2023.094
SP  - 1
EP  - 6
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka and Filipović, Branko",
year = "2023",
abstract = "Prostate health is related to the frequency of ejaculation and prostate drainage. Frequent, repetitive ejaculations theoretically may pose a prostate cancer risk due to ensuing high testosterone levels and loss of zinc. In line with this, the proximity of the human rectum and prostate is of diagnostic and therapeutic importance. We provide evidence to support the view that the proximity of the rectum and prostate in men enables lower bowel distention to compress the prostate's lateral side to facilitate drainage, which might lower cancer risk. This drainage mechanism is likely on-going and gradual, compared to rapid drainage following ejaculation. The physiological context suggests that these mechanisms of prostate drainage are complementary. Dietary content containing polyphenol- and fiber-rich meals, along with voluminous stool formation and increased gastrointestinal transit may all help improve prostate drainage. This may be important in voluntary abstinent men or other categories of sexually limited men. Recognizing that the anatomic relationship between the rectal area and prostate may facilitate prostate drainage, because of diet and gastro-intestinal activity, could be important to prostate health maintenance.",
publisher = "MRE Press",
journal = "Journal of Men's Health",
title = "The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health",
number = "10",
volume = "19",
doi = "10.22514/jomh.2023.094",
pages = "1-6"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B.,& Filipović, B.. (2023). The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health. in Journal of Men's Health
MRE Press., 19(10), 1-6.
https://doi.org/10.22514/jomh.2023.094

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B. The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health. in Journal of Men's Health. 2023;19(10):1-6.
doi:10.22514/jomh.2023.094 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka, Filipović, Branko, "The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health" in Journal of Men's Health, 19, no. 10 (2023):1-6,
https://doi.org/10.22514/jomh.2023.094 . .

TY  - CONF
AU  - Čukuranović-Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Stanković Đorević, Dobrila
AU  - Čukuranović, Rade
AU  - Milošević, Verica
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6229
AB  - Soy isoflavone daidzein exerts some beneficial effects in different ageing-related, chronic diseases and may represent a promising alternative to classical estrogenization of the prostate cancer suffering subjects. The aim of this study was to examine the effects of daidzein on the adrenocortical vascular network and hormonal output in a rat model of the mild andropause. Adult (3 months old) Wistar rats were divided into sham-operated (SO; n=7), orchidectomized (Orx; n=7) and daidzein treated orchidectomized (Orx+D; n=7) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Adrenocortical vascular network properties were identified using Novelli histochemical staining, vascular endothelial growth factor (VEGF) immunostaining and the newCast stereological software. Blood concentrations of aldosterone, corticosterone and DHEAS were measured by immunoassays. Volume densities of blood vessels and VEGF depots within the adrenal cortex were not significantly changed after daidzein treatment, although there was a tendency of decrease of these parameters. On the other hand, daidzein application increased (P<0.05) blood concentration of aldosterone by more than 2.5 times and 41%, in comparison with the same parameter in sham-operated and orchidectomized group, respectively. Corticosterone concentration in daidzein-treated, orchidectomized rats showed a tendency of elevation (P>0.05) compared to the both SO animals and orchidectomy alone. DHEAS blood concentration after daidzein application remained at the level of the Orx group, which in both cases ment elevation (P<0.05) by around 80% in comparison with the same parameter in SO group. We conclude that daidzein significantly increases aldosterone and to some lower extent corticosterone output in a rat model of the mild andropause, without significant rearrangements of the entire adrenal cortex vascular network.
PB  - bioscientifica
C3  - 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey
T1  - Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause
DO  - 10.1530/endoabs.90.EP63
SP  - 394
EP  - 395
ER  - 

@conference{
author = "Čukuranović-Kokoris, Jovana and Đorđević, Miodrag and Miler, Marko and Živanović, Jasmina and Marković Filipović, Jelena and Stanković Đorević, Dobrila and Čukuranović, Rade and Milošević, Verica and Filipović, Branko and Ajdžanović, Vladimir",
year = "2023",
abstract = "Soy isoflavone daidzein exerts some beneficial effects in different ageing-related, chronic diseases and may represent a promising alternative to classical estrogenization of the prostate cancer suffering subjects. The aim of this study was to examine the effects of daidzein on the adrenocortical vascular network and hormonal output in a rat model of the mild andropause. Adult (3 months old) Wistar rats were divided into sham-operated (SO; n=7), orchidectomized (Orx; n=7) and daidzein treated orchidectomized (Orx+D; n=7) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Adrenocortical vascular network properties were identified using Novelli histochemical staining, vascular endothelial growth factor (VEGF) immunostaining and the newCast stereological software. Blood concentrations of aldosterone, corticosterone and DHEAS were measured by immunoassays. Volume densities of blood vessels and VEGF depots within the adrenal cortex were not significantly changed after daidzein treatment, although there was a tendency of decrease of these parameters. On the other hand, daidzein application increased (P<0.05) blood concentration of aldosterone by more than 2.5 times and 41%, in comparison with the same parameter in sham-operated and orchidectomized group, respectively. Corticosterone concentration in daidzein-treated, orchidectomized rats showed a tendency of elevation (P>0.05) compared to the both SO animals and orchidectomy alone. DHEAS blood concentration after daidzein application remained at the level of the Orx group, which in both cases ment elevation (P<0.05) by around 80% in comparison with the same parameter in SO group. We conclude that daidzein significantly increases aldosterone and to some lower extent corticosterone output in a rat model of the mild andropause, without significant rearrangements of the entire adrenal cortex vascular network.",
publisher = "bioscientifica",
journal = "25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey",
title = "Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause",
doi = "10.1530/endoabs.90.EP63",
pages = "394-395"
}

Čukuranović-Kokoris, J., Đorđević, M., Miler, M., Živanović, J., Marković Filipović, J., Stanković Đorević, D., Čukuranović, R., Milošević, V., Filipović, B.,& Ajdžanović, V.. (2023). Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause. in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey
bioscientifica., 394-395.
https://doi.org/10.1530/endoabs.90.EP63

Čukuranović-Kokoris J, Đorđević M, Miler M, Živanović J, Marković Filipović J, Stanković Đorević D, Čukuranović R, Milošević V, Filipović B, Ajdžanović V. Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause. in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey. 2023;:394-395.
doi:10.1530/endoabs.90.EP63 .

Čukuranović-Kokoris, Jovana, Đorđević, Miodrag, Miler, Marko, Živanović, Jasmina, Marković Filipović, Jelena, Stanković Đorević, Dobrila, Čukuranović, Rade, Milošević, Verica, Filipović, Branko, Ajdžanović, Vladimir, "Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause" in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey (2023):394-395,
https://doi.org/10.1530/endoabs.90.EP63 . .

TY  - DATA
AU  - Miler, Marko
AU  - Živanović, Jasmina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6220
AB  - This dataset is a supplementary document of the research article: Lemon extract reduces hepatic oxidative stress and persulfidation levels by upregulating Nrf2 and Trx1 expression in old rats. It contains Table S1 with data about primer pairs used in qPCR analysis and Table S2 with data about antibodies used for western blot, immunofluorescent, and persulfidation analyses.
T1  - Supplementary material for the article: Lemon extract reduces hepatic oxidative stress and persulfidation levels by upregulating Nrf2 and Trx1 expression in old rats
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6220
ER  - 

@misc{
author = "Miler, Marko and Živanović, Jasmina",
year = "2023",
abstract = "This dataset is a supplementary document of the research article: Lemon extract reduces hepatic oxidative stress and persulfidation levels by upregulating Nrf2 and Trx1 expression in old rats. It contains Table S1 with data about primer pairs used in qPCR analysis and Table S2 with data about antibodies used for western blot, immunofluorescent, and persulfidation analyses.",
title = "Supplementary material for the article: Lemon extract reduces hepatic oxidative stress and persulfidation levels by upregulating Nrf2 and Trx1 expression in old rats",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6220"
}

Miler, M.,& Živanović, J.. (2023). Supplementary material for the article: Lemon extract reduces hepatic oxidative stress and persulfidation levels by upregulating Nrf2 and Trx1 expression in old rats. .
https://hdl.handle.net/21.15107/rcub_ibiss_6220

Miler M, Živanović J. Supplementary material for the article: Lemon extract reduces hepatic oxidative stress and persulfidation levels by upregulating Nrf2 and Trx1 expression in old rats. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6220 .

Miler, Marko, Živanović, Jasmina, "Supplementary material for the article: Lemon extract reduces hepatic oxidative stress and persulfidation levels by upregulating Nrf2 and Trx1 expression in old rats" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6220 .

TY  - CONF
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Miler, Marko
AU  - Manojlović-Stojanoski, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6695
AB  - Развој организма и хомеостатски механизми се прилагођавају променљивим условима спољашње средине још током пренаталног периода. Женски репродуктивни систем активно реагује адаптирајући развој и диференцирање гонадотропних ћелија хипофизе и оваријума у складу са условима околине.1,2 Различити типови стреса, укључујући хормонске, нутритивне и психолошке изазове могу проузроковати бројне промене које обликују будући репродуктивни капацитет женке и заправо су део пренаталног програмирања физиологије организма. Третман гравидних женки дексаметазоном (Дк) значајно утиче на развој фетуса и опонаша антенаталну терапију гравидних жена глукокортикоидима као најчешће присутни третман у обстетричкој пракси. Стога су гравидне женке пацова Дк третиране (3 x 0,5 мг/кг/тм Дк) од 16. до 18. дана гестације. Пренатално Дк излагање изазвало је смањење масе фетуса непосредно пред рођење. Забележено је значајно смањење апсолутног броја ФСХ (фоликулостимулирајући хормон) и ЛХ (лутеинизирајући хормон) гонадотропних ћелија хипофизе (p < 0,05) од феталног, преко постнаталног, до пуберталног периода у односу на контролне вредности. Паралелно, Дк излагање током феталног развоја проузроковало је смањење волумена оваријума код потомака старих 16 и 38 дана (p < 0,05). Број фоликула је смањен код 16 дана старих потомака, док су поремећај процеса фоликулогенезе и одложен почетак пубертета забележени код потомака старих 38 дана. Можемо закључити да пренатални период развоја снажно утиче на фертилитет женки током репродуктивног периода.
AB  - Razvoj organizma i homeostatski mehanizmi se prilagođavaju promenljivim uslovima spoljašnje sredine još tokom prenatalnog perioda. Ženski reproduktivni sistem aktivno reaguje adaptirajući razvoj i diferenciranje gonadotropnih ćelija hipofize i ovarijuma u skladu sa uslovima okoline.1,2 Različiti tipovi stresa, uključujući hormonske, nutritivne i psihološke izazove mogu prouzrokovati brojne promene koje oblikuju budući reproduktivni kapacitet ženke i zapravo su deo prenatalnog programiranja fiziologije organizma. Tretman gravidnih ženki deksametazonom (Dk) značajno utiče na razvoj fetusa i oponaša antenatalnu terapiju gravidnih žena glukokortikoidima kao najčešće prisutni tretman u obstetričkoj praksi. Stoga su gravidne ženke pacova Dk tretirane (3 x 0,5 mg/kg/tm Dk) od 16. do 18. dana gestacije. Prenatalno Dk izlaganje izazvalo je smanjenje mase fetusa neposredno pred rođenje. Zabeleženo je značajno smanjenje apsolutnog broja FSH (folikulostimulirajući hormon) i LH (luteinizirajući hormon) gonadotropnih ćelija hipofize (p < 0,05) od fetalnog, preko postnatalnog, do pubertalnog perioda u odnosu na kontrolne vrednosti. Paralelno, Dk izlaganje tokom fetalnog razvoja prouzrokovalo je smanjenje volumena ovarijuma kod potomaka starih 16 i 38 dana (p < 0,05). Broj folikula je smanjen kod 16 dana starih potomaka, dok su poremećaj procesa folikulogeneze i odložen početak puberteta zabeleženi kod potomaka starih 38 dana. Možemo zaključiti da prenatalni period razvoja snažno utiče na fertilitet ženki tokom reproduktivnog perioda.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Развој женског репродуктивног система и последице пренаталног излагања дексаметазону
T1  - Razvoj ženskog reproduktivnog sistema i posledice prenatalnog izlaganja deksametazonu
SP  - 379
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6695
ER  - 

@conference{
author = "Ristić, Nataša and Nestorović, Nataša and Trifunović, Svetlana and Šošić-Jurjević, Branka and Filipović, Branko and Ajdžanović, Vladimir and Živanović, Jasmina and Miler, Marko and Manojlović-Stojanoski, Milica",
year = "2022",
abstract = "Развој организма и хомеостатски механизми се прилагођавају променљивим условима спољашње средине још током пренаталног периода. Женски репродуктивни систем активно реагује адаптирајући развој и диференцирање гонадотропних ћелија хипофизе и оваријума у складу са условима околине.1,2 Различити типови стреса, укључујући хормонске, нутритивне и психолошке изазове могу проузроковати бројне промене које обликују будући репродуктивни капацитет женке и заправо су део пренаталног програмирања физиологије организма. Третман гравидних женки дексаметазоном (Дк) значајно утиче на развој фетуса и опонаша антенаталну терапију гравидних жена глукокортикоидима као најчешће присутни третман у обстетричкој пракси. Стога су гравидне женке пацова Дк третиране (3 x 0,5 мг/кг/тм Дк) од 16. до 18. дана гестације. Пренатално Дк излагање изазвало је смањење масе фетуса непосредно пред рођење. Забележено је значајно смањење апсолутног броја ФСХ (фоликулостимулирајући хормон) и ЛХ (лутеинизирајући хормон) гонадотропних ћелија хипофизе (p < 0,05) од феталног, преко постнаталног, до пуберталног периода у односу на контролне вредности. Паралелно, Дк излагање током феталног развоја проузроковало је смањење волумена оваријума код потомака старих 16 и 38 дана (p < 0,05). Број фоликула је смањен код 16 дана старих потомака, док су поремећај процеса фоликулогенезе и одложен почетак пубертета забележени код потомака старих 38 дана. Можемо закључити да пренатални период развоја снажно утиче на фертилитет женки током репродуктивног периода., Razvoj organizma i homeostatski mehanizmi se prilagođavaju promenljivim uslovima spoljašnje sredine još tokom prenatalnog perioda. Ženski reproduktivni sistem aktivno reaguje adaptirajući razvoj i diferenciranje gonadotropnih ćelija hipofize i ovarijuma u skladu sa uslovima okoline.1,2 Različiti tipovi stresa, uključujući hormonske, nutritivne i psihološke izazove mogu prouzrokovati brojne promene koje oblikuju budući reproduktivni kapacitet ženke i zapravo su deo prenatalnog programiranja fiziologije organizma. Tretman gravidnih ženki deksametazonom (Dk) značajno utiče na razvoj fetusa i oponaša antenatalnu terapiju gravidnih žena glukokortikoidima kao najčešće prisutni tretman u obstetričkoj praksi. Stoga su gravidne ženke pacova Dk tretirane (3 x 0,5 mg/kg/tm Dk) od 16. do 18. dana gestacije. Prenatalno Dk izlaganje izazvalo je smanjenje mase fetusa neposredno pred rođenje. Zabeleženo je značajno smanjenje apsolutnog broja FSH (folikulostimulirajući hormon) i LH (luteinizirajući hormon) gonadotropnih ćelija hipofize (p < 0,05) od fetalnog, preko postnatalnog, do pubertalnog perioda u odnosu na kontrolne vrednosti. Paralelno, Dk izlaganje tokom fetalnog razvoja prouzrokovalo je smanjenje volumena ovarijuma kod potomaka starih 16 i 38 dana (p < 0,05). Broj folikula je smanjen kod 16 dana starih potomaka, dok su poremećaj procesa folikulogeneze i odložen početak puberteta zabeleženi kod potomaka starih 38 dana. Možemo zaključiti da prenatalni period razvoja snažno utiče na fertilitet ženki tokom reproduktivnog perioda.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Развој женског репродуктивног система и последице пренаталног излагања дексаметазону, Razvoj ženskog reproduktivnog sistema i posledice prenatalnog izlaganja deksametazonu",
pages = "379",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6695"
}

Ristić, N., Nestorović, N., Trifunović, S., Šošić-Jurjević, B., Filipović, B., Ajdžanović, V., Živanović, J., Miler, M.,& Manojlović-Stojanoski, M.. (2022). Развој женског репродуктивног система и последице пренаталног излагања дексаметазону. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 379.
https://hdl.handle.net/21.15107/rcub_ibiss_6695

Ristić N, Nestorović N, Trifunović S, Šošić-Jurjević B, Filipović B, Ajdžanović V, Živanović J, Miler M, Manojlović-Stojanoski M. Развој женског репродуктивног система и последице пренаталног излагања дексаметазону. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:379.
https://hdl.handle.net/21.15107/rcub_ibiss_6695 .

Ristić, Nataša, Nestorović, Nataša, Trifunović, Svetlana, Šošić-Jurjević, Branka, Filipović, Branko, Ajdžanović, Vladimir, Živanović, Jasmina, Miler, Marko, Manojlović-Stojanoski, Milica, "Развој женског репродуктивног система и последице пренаталног излагања дексаметазону" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):379,
https://hdl.handle.net/21.15107/rcub_ibiss_6695 .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Kojić, Danijela
AU  - Miler, Marko
AU  - Karan, Jelena
AU  - Ivelja, Ivana
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.hbd-sbc.hr/kongres2022/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6564
AB  - Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.
PB  - Zagreb: Hrvatsko biološko društvo
C3  - Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska
T1  - Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes
SP  - 231
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6564
ER  - 

@conference{
author = "Marković Filipović, Jelena and Kojić, Danijela and Miler, Marko and Karan, Jelena and Ivelja, Ivana and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.",
publisher = "Zagreb: Hrvatsko biološko društvo",
journal = "Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska",
title = "Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes",
pages = "231-232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6564"
}

Marković Filipović, J., Kojić, D., Miler, M., Karan, J., Ivelja, I.,& Matavulj, M.. (2022). Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes. in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska
Zagreb: Hrvatsko biološko društvo., 231-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6564

Marković Filipović J, Kojić D, Miler M, Karan J, Ivelja I, Matavulj M. Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes. in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska. 2022;:231-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6564 .

Marković Filipović, Jelena, Kojić, Danijela, Miler, Marko, Karan, Jelena, Ivelja, Ivana, Matavulj, Milica, "Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes" in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska (2022):231-232,
https://hdl.handle.net/21.15107/rcub_ibiss_6564 .

TY  - JOUR
AU  - Marković-Filipović, Jelena
AU  - Miler, Marko
AU  - Kojić, Danijela
AU  - Andrejić Višnjić, Bojana
AU  - Milošević, Verica
AU  - Cukuranovic Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Matavulj, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5480
AB  - Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory processes. In addition, AA is formed during processing of starchy food at high temperature. The aim of our study was to examine effects of subchronic AA treatment on adult rat liver using histological, stereological and biochemical methods. Adult male Wistar rats were treated with AA at doses of 25 mg/kg b.w. and 50 mg/kg b.w. for three weeks. Stereological analysis showed decrease of volume density of hepatocyte cytoplasm, and increase of volume density of hepatocyte nuclei and nucleocytoplasmic ratio in AA50mg group. Immunohistochemical analysis of the liver sections showed that treatment with AA50mg increase the percentage of PCNA positive cells, while the percentage of caspase 3 positive cells was not affected by AA. PAS-staining showed that glycogen content in hepatocytes was not affected by AA. Serological examination revealed increase of lipid peroxidation in AA50mg group, while total protein concentration, protein thiol group level, as well as, paraoxonase 1 activity were not changed in AA- exposed animals. Stereological and immunohistochemical analyses of adult liver sections suggest increase of proliferation in AA50mg group, while increase of lipid peroxidation in serum of AA50mg group indicates oxidative stress induction.
T2  - International Journal of Morphology
T1  - Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study
IS  - 6
VL  - 40
DO  - 10.4067/s0717-95022022000601618
SP  - 1618
EP  - 1623
ER  - 

@article{
author = "Marković-Filipović, Jelena and Miler, Marko and Kojić, Danijela and Andrejić Višnjić, Bojana and Milošević, Verica and Cukuranovic Kokoris, Jovana and Đorđević, Miodrag and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory processes. In addition, AA is formed during processing of starchy food at high temperature. The aim of our study was to examine effects of subchronic AA treatment on adult rat liver using histological, stereological and biochemical methods. Adult male Wistar rats were treated with AA at doses of 25 mg/kg b.w. and 50 mg/kg b.w. for three weeks. Stereological analysis showed decrease of volume density of hepatocyte cytoplasm, and increase of volume density of hepatocyte nuclei and nucleocytoplasmic ratio in AA50mg group. Immunohistochemical analysis of the liver sections showed that treatment with AA50mg increase the percentage of PCNA positive cells, while the percentage of caspase 3 positive cells was not affected by AA. PAS-staining showed that glycogen content in hepatocytes was not affected by AA. Serological examination revealed increase of lipid peroxidation in AA50mg group, while total protein concentration, protein thiol group level, as well as, paraoxonase 1 activity were not changed in AA- exposed animals. Stereological and immunohistochemical analyses of adult liver sections suggest increase of proliferation in AA50mg group, while increase of lipid peroxidation in serum of AA50mg group indicates oxidative stress induction.",
journal = "International Journal of Morphology",
title = "Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study",
number = "6",
volume = "40",
doi = "10.4067/s0717-95022022000601618",
pages = "1618-1623"
}

Marković-Filipović, J., Miler, M., Kojić, D., Andrejić Višnjić, B., Milošević, V., Cukuranovic Kokoris, J., Đorđević, M.,& Matavulj, M.. (2022). Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study. in International Journal of Morphology, 40(6), 1618-1623.
https://doi.org/10.4067/s0717-95022022000601618

Marković-Filipović J, Miler M, Kojić D, Andrejić Višnjić B, Milošević V, Cukuranovic Kokoris J, Đorđević M, Matavulj M. Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study. in International Journal of Morphology. 2022;40(6):1618-1623.
doi:10.4067/s0717-95022022000601618 .

Marković-Filipović, Jelena, Miler, Marko, Kojić, Danijela, Andrejić Višnjić, Bojana, Milošević, Verica, Cukuranovic Kokoris, Jovana, Đorđević, Miodrag, Matavulj, Milica, "Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study" in International Journal of Morphology, 40, no. 6 (2022):1618-1623,
https://doi.org/10.4067/s0717-95022022000601618 . .

TY  - JOUR
AU  - Marković Filipović, Jelena
AU  - Miler, Marko
AU  - Kojić, Danijela
AU  - Karan, Jelena
AU  - Ivelja, Ivana
AU  - Čukuranović Kokoris, Jovana
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/11/6062
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5007
AB  - Acrylamide (AA) toxicity is associated with oxidative stress. During detoxification, AA is either coupled to gluthatione or biotransformed to glycidamide by the enzyme cytochrome P450 2E1 (CYP2E1). The aim of our study was to examine the hepatotoxicity of AA in vivo and in vitro. Thirty male Wistar rats were treated with 25 or 50 mg/kg b.w. of AA for 3 weeks. Qualitative and quantitative immunohistochemical evaluation of inducible nitric oxide synthase (iNOS), CYP2E1, catalase (CAT), superoxide dismutase 1 (SOD1), and SOD2 expression in liver was carried out. Bearing in mind that the liver is consisted mainly of hepatocytes, in a parallel study, we used the rat hepatoma cell line H4IIE to investigate the effects of AA at IC20 and IC50 concentrations on the redox status and the activity of CAT, SOD, and glutathione-S-transferase (GST), their gene expression, and CYP2E1 and iNOS expression. Immunohistochemically stained liver sections showed that treatment with AA25mg induced a significant decrease of CYP2E1 protein expression (p < 0.05), while treatment with AA50mg led to a significant increase of iNOS protein expression (p < 0.05). AA treatment dose-dependently elevated SOD2 protein expression (p < 0.05), while SOD1 protein expression was significantly increased only at AA50mg (p < 0.05). CAT protein expression was not significantly affected by AA treatments (p > 0.05). In AA-treated H4IIE cells, a concentration-dependent significant increase in lipid peroxidation and nitrite levels was observed (p < 0.05), while GSH content and SOD activity significantly decreased in a concentration-dependent manner (p < 0.05). AA IC50 significantly enhanced GST activity (p < 0.05). The level of mRNA significantly increased in a concentration-dependent manner for iNOS, SOD2, and CAT in AA-treated H4IIE cells (p < 0.05). AA IC50 significantly increased the transcription of SOD1, GSTA2, and GSTP1 genes (p < 0.05), while AA IC20 significantly decreased mRNA for CYP2E1 in H4IIE cells (p < 0.05). Obtained results indicate that AA treatments, both in vivo and in vitro, change hepatocytes; drug-metabolizing potential and disturb its redox status.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.
IS  - 11
VL  - 23
DO  - 10.3390/ijms23116062
SP  - 6062
ER  - 

@article{
author = "Marković Filipović, Jelena and Miler, Marko and Kojić, Danijela and Karan, Jelena and Ivelja, Ivana and Čukuranović Kokoris, Jovana and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) toxicity is associated with oxidative stress. During detoxification, AA is either coupled to gluthatione or biotransformed to glycidamide by the enzyme cytochrome P450 2E1 (CYP2E1). The aim of our study was to examine the hepatotoxicity of AA in vivo and in vitro. Thirty male Wistar rats were treated with 25 or 50 mg/kg b.w. of AA for 3 weeks. Qualitative and quantitative immunohistochemical evaluation of inducible nitric oxide synthase (iNOS), CYP2E1, catalase (CAT), superoxide dismutase 1 (SOD1), and SOD2 expression in liver was carried out. Bearing in mind that the liver is consisted mainly of hepatocytes, in a parallel study, we used the rat hepatoma cell line H4IIE to investigate the effects of AA at IC20 and IC50 concentrations on the redox status and the activity of CAT, SOD, and glutathione-S-transferase (GST), their gene expression, and CYP2E1 and iNOS expression. Immunohistochemically stained liver sections showed that treatment with AA25mg induced a significant decrease of CYP2E1 protein expression (p < 0.05), while treatment with AA50mg led to a significant increase of iNOS protein expression (p < 0.05). AA treatment dose-dependently elevated SOD2 protein expression (p < 0.05), while SOD1 protein expression was significantly increased only at AA50mg (p < 0.05). CAT protein expression was not significantly affected by AA treatments (p > 0.05). In AA-treated H4IIE cells, a concentration-dependent significant increase in lipid peroxidation and nitrite levels was observed (p < 0.05), while GSH content and SOD activity significantly decreased in a concentration-dependent manner (p < 0.05). AA IC50 significantly enhanced GST activity (p < 0.05). The level of mRNA significantly increased in a concentration-dependent manner for iNOS, SOD2, and CAT in AA-treated H4IIE cells (p < 0.05). AA IC50 significantly increased the transcription of SOD1, GSTA2, and GSTP1 genes (p < 0.05), while AA IC20 significantly decreased mRNA for CYP2E1 in H4IIE cells (p < 0.05). Obtained results indicate that AA treatments, both in vivo and in vitro, change hepatocytes; drug-metabolizing potential and disturb its redox status.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.",
number = "11",
volume = "23",
doi = "10.3390/ijms23116062",
pages = "6062"
}

Marković Filipović, J., Miler, M., Kojić, D., Karan, J., Ivelja, I., Čukuranović Kokoris, J.,& Matavulj, M.. (2022). Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.. in International Journal of Molecular Sciences
Basel: MDPI., 23(11), 6062.
https://doi.org/10.3390/ijms23116062

Marković Filipović J, Miler M, Kojić D, Karan J, Ivelja I, Čukuranović Kokoris J, Matavulj M. Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.. in International Journal of Molecular Sciences. 2022;23(11):6062.
doi:10.3390/ijms23116062 .

Marković Filipović, Jelena, Miler, Marko, Kojić, Danijela, Karan, Jelena, Ivelja, Ivana, Čukuranović Kokoris, Jovana, Matavulj, Milica, "Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes." in International Journal of Molecular Sciences, 23, no. 11 (2022):6062,
https://doi.org/10.3390/ijms23116062 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Trifunović, Svetlana
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Ristić, Nataša
AU  - Filipović, Branko
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/2/791
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35054977
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4769
AB  - Vitamin D plays an essential role in prevention and treatment of osteoporosis. Thyroid hormones, in addition to vitamin D, significantly contribute to regulation of bone remodeling cycle and health. There is currently no data about a possible connection between vitamin D treatment and the thyroid in the context of osteoporosis. Middle-aged Wistar rats were divided into: sham operated (SO), orchidectomized (Orx), and cholecalciferol-treated orchidectomized (Orx + Vit. D3; 5 µg/kg b.m./day during three weeks) groups (n = 6/group). Concentration of 25(OH)D in serum of the Orx + Vit. D3 group increased 4 and 3.2 times (p < 0.0001) respectively, compared to Orx and SO group. T4, TSH, and calcitonin in serum remained unaltered. Vit. D3 treatment induced changes in thyroid functional morphology that indicate increased utilization of stored colloid and release of thyroid hormones in comparison with hormone synthesis, to maintain hormonal balance. Increased expression of nuclear VDR (p < 0.05) points to direct, TSH independent action of Vit. D on thyrocytes. Strong CYP24A1 immunostaining in C cells suggests its prominent expression in response to Vit. D in this cell subpopulation in orchidectomized rat model of osteoporosis. The indirect effect of Vit. D on bone, through fine regulation of thyroid function, is small.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.
IS  - 2
VL  - 23
DO  - 10.3390/ijms23020791
SP  - 791
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Trifunović, Svetlana and Živanović, Jasmina and Ajdžanović, Vladimir and Miler, Marko and Ristić, Nataša and Filipović, Branko",
year = "2022",
abstract = "Vitamin D plays an essential role in prevention and treatment of osteoporosis. Thyroid hormones, in addition to vitamin D, significantly contribute to regulation of bone remodeling cycle and health. There is currently no data about a possible connection between vitamin D treatment and the thyroid in the context of osteoporosis. Middle-aged Wistar rats were divided into: sham operated (SO), orchidectomized (Orx), and cholecalciferol-treated orchidectomized (Orx + Vit. D3; 5 µg/kg b.m./day during three weeks) groups (n = 6/group). Concentration of 25(OH)D in serum of the Orx + Vit. D3 group increased 4 and 3.2 times (p < 0.0001) respectively, compared to Orx and SO group. T4, TSH, and calcitonin in serum remained unaltered. Vit. D3 treatment induced changes in thyroid functional morphology that indicate increased utilization of stored colloid and release of thyroid hormones in comparison with hormone synthesis, to maintain hormonal balance. Increased expression of nuclear VDR (p < 0.05) points to direct, TSH independent action of Vit. D on thyrocytes. Strong CYP24A1 immunostaining in C cells suggests its prominent expression in response to Vit. D in this cell subpopulation in orchidectomized rat model of osteoporosis. The indirect effect of Vit. D on bone, through fine regulation of thyroid function, is small.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.",
number = "2",
volume = "23",
doi = "10.3390/ijms23020791",
pages = "791"
}

Šošić-Jurjević, B., Trifunović, S., Živanović, J., Ajdžanović, V., Miler, M., Ristić, N.,& Filipović, B.. (2022). Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.. in International Journal of Molecular Sciences
Basel: MDPI., 23(2), 791.
https://doi.org/10.3390/ijms23020791

Šošić-Jurjević B, Trifunović S, Živanović J, Ajdžanović V, Miler M, Ristić N, Filipović B. Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.. in International Journal of Molecular Sciences. 2022;23(2):791.
doi:10.3390/ijms23020791 .

Šošić-Jurjević, Branka , Trifunović, Svetlana, Živanović, Jasmina, Ajdžanović, Vladimir, Miler, Marko, Ristić, Nataša, Filipović, Branko, "Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis." in International Journal of Molecular Sciences, 23, no. 2 (2022):791,
https://doi.org/10.3390/ijms23020791 . .

TY  - JOUR
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Stanković, Sanja
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S094096022100162X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4499
AB  - Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen – activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis.
PB  - Elsevier GmbH
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause
VL  - 239
DO  - 10.1016/j.aanat.2021.151836
SP  - 151836
ER  - 

@article{
author = "Živanović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir and Miler, Marko and Stanković, Sanja and Milošević, Verica and Filipović, Branko",
year = "2022",
abstract = "Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen – activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis.",
publisher = "Elsevier GmbH",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause",
volume = "239",
doi = "10.1016/j.aanat.2021.151836",
pages = "151836"
}

Živanović, J., Jarić, I., Ajdžanović, V., Miler, M., Stanković, S., Milošević, V.,& Filipović, B.. (2022). Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause. in Annals of Anatomy - Anatomischer Anzeiger
Elsevier GmbH., 239, 151836.
https://doi.org/10.1016/j.aanat.2021.151836

Živanović J, Jarić I, Ajdžanović V, Miler M, Stanković S, Milošević V, Filipović B. Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause. in Annals of Anatomy - Anatomischer Anzeiger. 2022;239:151836.
doi:10.1016/j.aanat.2021.151836 .

Živanović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, Miler, Marko, Stanković, Sanja, Milošević, Verica, Filipović, Branko, "Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause" in Annals of Anatomy - Anatomischer Anzeiger, 239 (2022):151836,
https://doi.org/10.1016/j.aanat.2021.151836 . .

TY  - CONF
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ristić, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Šošić-Jurjević, Branka 
AU  - Nestorović, Nataša
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
PY  - 2022
UR  - https://www.serbiosoc.org.rs/?page_id=702
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6034
AB  - Старење доводи до акумулације и смањене елиминације реактивних врста,
стварајући оксидациону средину у јетри. Једна од могућих опција за одлагање овог
догађаја је употреба антиоксиданата из цитруса у склопу процеса здравог старења.
Стога, ова студија je имала за циљ да истражи ефекте ериоцитрина (ЕРИ),
полифенола из лимуна, на редокс средину у јетри код 24-месечних мужјака Wistar
пацова. ЕРИ (30 мг/кг т.м.) је даван орално, једном дневно током четири недеље.
Контролне групе су примиле или носач третмана (сунцокретово уље) или су остале
интактне. Примењена методологија подразумевала је имуноблот и qPCR анализу,
детекцију нивоа персулфидације у гелу и биохемијске анализе. Третман ЕРИ
повећао је експресију гена и протеина за нуклеарни фактор 2 (Nrf2), тиоредоксин
(Trx) 1, глутатион пероксидазу и редуктазу. Експресија гена супероксид дисмутазе
2 (SOD2) и Trx2 је смањена, док је примена ЕРИ повећала експресију протеина
SOD2. Штавише, ЕРИ је смањио концентрацију малондиалдехида, маркера
оксидативног оштећења у ћелији. Показали смо смањење персулфидације протеина
у јетри након третмана ЕРИ, док су генска и протеинска експресија ензима који
производе H2S остале непромењене. У закључку, ЕРИ појачава регулацију редокс
регулатора јетре Nrf2, који активира антиоксидативни одбрамбени систем и Trx1,
што доводи до смањења нивоа персулфидације протеина у јетри. Наши налази
указују на потенцијал ЕРИ у унапређењу редокс средине у јетри старих пацова.
AB  - Starenje dovodi do akumulacije i smanjene eliminacije reaktivnih vrsta,
stvarajući oksidacionu sredinu u jetri. Jedna od mogućih opcija za odlaganje ovog
događaja je upotreba antioksidanata iz citrusa u sklopu procesa zdravog starenja.
Stoga, ova studija je imala za cilj da istraži efekte eriocitrina (ERI),
polifenola iz limuna, na redoks sredinu u jetri kod 24-mesečnih mužjaka Wistar
pacova. ERI (30 mg/kg t.m.) je davan oralno, jednom dnevno tokom četiri nedelje.
Kontrolne grupe su primile ili nosač tretmana (suncokretovo ulje) ili su ostale
intaktne. Primenjena metodologija podrazumevala je imunoblot i qPCR analizu,
detekciju nivoa persulfidacije u gelu i biohemijske analize. Tretman ERI
povećao je ekspresiju gena i proteina za nuklearni faktor 2 (Nrf2), tioredoksin
(Trx) 1, glutation peroksidazu i reduktazu. Ekspresija gena superoksid dismutaze
2 (SOD2) i Trx2 je smanjena, dok je primena ERI povećala ekspresiju proteina
SOD2. Štaviše, ERI je smanjio koncentraciju malondialdehida, markera
oksidativnog oštećenja u ćeliji. Pokazali smo smanjenje persulfidacije proteina
u jetri nakon tretmana ERI, dok su genska i proteinska ekspresija enzima koji
proizvode H2S ostale nepromenjene. U zaključku, ERI pojačava regulaciju redoks
regulatora jetre Nrf2, koji aktivira antioksidativni odbrambeni sistem i Trx1,
što dovodi do smanjenja nivoa persulfidacije proteina u jetri. Naši nalazi
ukazuju na potencijal ERI u unapređenju redoks sredine u jetri starih pacova.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова
T1  - Eriocitrin, polifenol iz limuna, unapređuje redoks sredinu u jetri starih pacova
SP  - 374
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6034
ER  - 

@conference{
author = "Miler, Marko and Živanović, Jasmina and Ristić, Nataša and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Šošić-Jurjević, Branka  and Nestorović, Nataša and Filipović, Branko and Ajdžanović, Vladimir",
year = "2022",
abstract = "Старење доводи до акумулације и смањене елиминације реактивних врста,
стварајући оксидациону средину у јетри. Једна од могућих опција за одлагање овог
догађаја је употреба антиоксиданата из цитруса у склопу процеса здравог старења.
Стога, ова студија je имала за циљ да истражи ефекте ериоцитрина (ЕРИ),
полифенола из лимуна, на редокс средину у јетри код 24-месечних мужјака Wistar
пацова. ЕРИ (30 мг/кг т.м.) је даван орално, једном дневно током четири недеље.
Контролне групе су примиле или носач третмана (сунцокретово уље) или су остале
интактне. Примењена методологија подразумевала је имуноблот и qPCR анализу,
детекцију нивоа персулфидације у гелу и биохемијске анализе. Третман ЕРИ
повећао је експресију гена и протеина за нуклеарни фактор 2 (Nrf2), тиоредоксин
(Trx) 1, глутатион пероксидазу и редуктазу. Експресија гена супероксид дисмутазе
2 (SOD2) и Trx2 је смањена, док је примена ЕРИ повећала експресију протеина
SOD2. Штавише, ЕРИ је смањио концентрацију малондиалдехида, маркера
оксидативног оштећења у ћелији. Показали смо смањење персулфидације протеина
у јетри након третмана ЕРИ, док су генска и протеинска експресија ензима који
производе H2S остале непромењене. У закључку, ЕРИ појачава регулацију редокс
регулатора јетре Nrf2, који активира антиоксидативни одбрамбени систем и Trx1,
што доводи до смањења нивоа персулфидације протеина у јетри. Наши налази
указују на потенцијал ЕРИ у унапређењу редокс средине у јетри старих пацова., Starenje dovodi do akumulacije i smanjene eliminacije reaktivnih vrsta,
stvarajući oksidacionu sredinu u jetri. Jedna od mogućih opcija za odlaganje ovog
događaja je upotreba antioksidanata iz citrusa u sklopu procesa zdravog starenja.
Stoga, ova studija je imala za cilj da istraži efekte eriocitrina (ERI),
polifenola iz limuna, na redoks sredinu u jetri kod 24-mesečnih mužjaka Wistar
pacova. ERI (30 mg/kg t.m.) je davan oralno, jednom dnevno tokom četiri nedelje.
Kontrolne grupe su primile ili nosač tretmana (suncokretovo ulje) ili su ostale
intaktne. Primenjena metodologija podrazumevala je imunoblot i qPCR analizu,
detekciju nivoa persulfidacije u gelu i biohemijske analize. Tretman ERI
povećao je ekspresiju gena i proteina za nuklearni faktor 2 (Nrf2), tioredoksin
(Trx) 1, glutation peroksidazu i reduktazu. Ekspresija gena superoksid dismutaze
2 (SOD2) i Trx2 je smanjena, dok je primena ERI povećala ekspresiju proteina
SOD2. Štaviše, ERI je smanjio koncentraciju malondialdehida, markera
oksidativnog oštećenja u ćeliji. Pokazali smo smanjenje persulfidacije proteina
u jetri nakon tretmana ERI, dok su genska i proteinska ekspresija enzima koji
proizvode H2S ostale nepromenjene. U zaključku, ERI pojačava regulaciju redoks
regulatora jetre Nrf2, koji aktivira antioksidativni odbrambeni sistem i Trx1,
što dovodi do smanjenja nivoa persulfidacije proteina u jetri. Naši nalazi
ukazuju na potencijal ERI u unapređenju redoks sredine u jetri starih pacova.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова, Eriocitrin, polifenol iz limuna, unapređuje redoks sredinu u jetri starih pacova",
pages = "374",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6034"
}

Miler, M., Živanović, J., Ristić, N., Manojlović-Stojanoski, M., Trifunović, S., Šošić-Jurjević, B., Nestorović, N., Filipović, B.,& Ajdžanović, V.. (2022). Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 374.
https://hdl.handle.net/21.15107/rcub_ibiss_6034

Miler M, Živanović J, Ristić N, Manojlović-Stojanoski M, Trifunović S, Šošić-Jurjević B, Nestorović N, Filipović B, Ajdžanović V. Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:374.
https://hdl.handle.net/21.15107/rcub_ibiss_6034 .

Miler, Marko, Živanović, Jasmina, Ristić, Nataša, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Šošić-Jurjević, Branka , Nestorović, Nataša, Filipović, Branko, Ajdžanović, Vladimir, "Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):374,
https://hdl.handle.net/21.15107/rcub_ibiss_6034 .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Ivelja, Ivana
AU  - Karan, Jelena
AU  - Miler, Marko
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.serbiosoc.org.rs/?page_id=702
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6036
AB  - Akrilamid (AA) je toksična industrijska hemikalija čiji se metabolizam odvija u jetri. Dodatno, AA se nalazi u često konzumiranoj hrani bogatoj ugljenim hidratima koja se sprema na visokim temperaturama. Cilj naše studije je da ispitamo efekat AA na proliferativne i apoptotičke karakteristike jetre odraslih pacova, koristeći imunohistohemijske (IHC) metode. Odrasli mužjaci pacova soja Wistar, stari 65 dana tretirani su dozama AA od 25 mg/kg i 50 mg/kg. Tkivo prethodno fiksirano u formalinu i ukalupljeno u parafin, isečeno je na preseke debljine 5 µm i obojeno imunohistohemijski koristeći anti-PCNA i anticaspase 3 antitela. Procenat obojene površine imunohistohemijski obojenih preseka određen je pomoću ImageJ dodatka – IHC profiler. U kontrolnoj grupi i grupama tretiranim akrilamidom ekspresija PCNA primećena je u jedrima hepatocita. AA tretman dovodi do povećanja brojnosti PCNA pozitivnih ćelija koje je zavisno od doze. Povećanje brojnosti PCNA pozitivnih ćelija pokazalo je i statističku značajnost u grupi tretiranoj sa AA50mg. U svim grupama, imunobojenje kaspazom 3 pokazalo je slabu citoplazmatsku imunoreaktivnost. Statistička analiza ukazala je na to da AA tretman nije značajno uticao na procenat ćelija pozitivnih na kaspazu 3. Imunohistohemijska analiza preseka jetre pokazala je da AA50mg tretman utiče na proliferativnost, ali ne i apoptozu hepatocita pacova.
AB  - Акриламид (АА) је токсична индустријска хемикалија чији се метаболизам одвија
у јетри. Додатно, АА се налази у често конзумираној храни богатој угљеним
хидратима која се спрема на високим температурама. Циљ наше студије је да
испитамо ефекат АА на пролиферативне и апоптотичке карактеристике јетре
одраслих пацова, користећи имунохистохемијске (ИХЦ) методе. Одрасли мужјаци
пацова соја Wistar, стари 65 дана третирани су дозама АА од 25 мг/кг и 50 мг/кг.
Ткиво претходно фиксирано у формалину и укалупљено у парафин, исечено је на
пресеке дебљине 5 µm и обојено имунохистохемијски користећи анти-PCNA и anticaspase 3 антитела. Проценат обојене површине имунохистохемијски обојених
пресека одређен је помоћу ImageJ додатка – IHC profiler. У контролној групи и
групама третираним акриламидом експресија PCNA примећена је у једрима
хепатоцита. АА третман доводи до повећања бројности PCNA позитивних ћелија
које је зависно од дозе. Повећање бројности PCNA позитивних ћелија показало је и
статистичку значајност у групи третираној са AA50мг. У свим групама, имунобојење
каспазом 3 показало је слабу цитоплазматску имунореактивност. Статистичка
анализа указала је на то да АА третман није значајно утицао на проценат ћелија
позитивних на каспазу 3. Имунохистохемијска анализа пресека јетре показала је да
AA50мг третман утиче на пролиферативност, али не и апоптозу хепатоцита пацова.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova
T1  - Ефекат третмана акриламидом на пролиферативне и апоптотичке карактеристике јетре одраслих пацова
SP  - 366
EP  - 366
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6036
ER  - 

@conference{
author = "Marković Filipović, Jelena and Ivelja, Ivana and Karan, Jelena and Miler, Marko and Matavulj, Milica",
year = "2022",
abstract = "Akrilamid (AA) je toksična industrijska hemikalija čiji se metabolizam odvija u jetri. Dodatno, AA se nalazi u često konzumiranoj hrani bogatoj ugljenim hidratima koja se sprema na visokim temperaturama. Cilj naše studije je da ispitamo efekat AA na proliferativne i apoptotičke karakteristike jetre odraslih pacova, koristeći imunohistohemijske (IHC) metode. Odrasli mužjaci pacova soja Wistar, stari 65 dana tretirani su dozama AA od 25 mg/kg i 50 mg/kg. Tkivo prethodno fiksirano u formalinu i ukalupljeno u parafin, isečeno je na preseke debljine 5 µm i obojeno imunohistohemijski koristeći anti-PCNA i anticaspase 3 antitela. Procenat obojene površine imunohistohemijski obojenih preseka određen je pomoću ImageJ dodatka – IHC profiler. U kontrolnoj grupi i grupama tretiranim akrilamidom ekspresija PCNA primećena je u jedrima hepatocita. AA tretman dovodi do povećanja brojnosti PCNA pozitivnih ćelija koje je zavisno od doze. Povećanje brojnosti PCNA pozitivnih ćelija pokazalo je i statističku značajnost u grupi tretiranoj sa AA50mg. U svim grupama, imunobojenje kaspazom 3 pokazalo je slabu citoplazmatsku imunoreaktivnost. Statistička analiza ukazala je na to da AA tretman nije značajno uticao na procenat ćelija pozitivnih na kaspazu 3. Imunohistohemijska analiza preseka jetre pokazala je da AA50mg tretman utiče na proliferativnost, ali ne i apoptozu hepatocita pacova., Акриламид (АА) је токсична индустријска хемикалија чији се метаболизам одвија
у јетри. Додатно, АА се налази у често конзумираној храни богатој угљеним
хидратима која се спрема на високим температурама. Циљ наше студије је да
испитамо ефекат АА на пролиферативне и апоптотичке карактеристике јетре
одраслих пацова, користећи имунохистохемијске (ИХЦ) методе. Одрасли мужјаци
пацова соја Wistar, стари 65 дана третирани су дозама АА од 25 мг/кг и 50 мг/кг.
Ткиво претходно фиксирано у формалину и укалупљено у парафин, исечено је на
пресеке дебљине 5 µm и обојено имунохистохемијски користећи анти-PCNA и anticaspase 3 антитела. Проценат обојене површине имунохистохемијски обојених
пресека одређен је помоћу ImageJ додатка – IHC profiler. У контролној групи и
групама третираним акриламидом експресија PCNA примећена је у једрима
хепатоцита. АА третман доводи до повећања бројности PCNA позитивних ћелија
које је зависно од дозе. Повећање бројности PCNA позитивних ћелија показало је и
статистичку значајност у групи третираној са AA50мг. У свим групама, имунобојење
каспазом 3 показало је слабу цитоплазматску имунореактивност. Статистичка
анализа указала је на то да АА третман није значајно утицао на проценат ћелија
позитивних на каспазу 3. Имунохистохемијска анализа пресека јетре показала је да
AA50мг третман утиче на пролиферативност, али не и апоптозу хепатоцита пацова.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova, Ефекат третмана акриламидом на пролиферативне и апоптотичке карактеристике јетре одраслих пацова",
pages = "366-366",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6036"
}

Marković Filipović, J., Ivelja, I., Karan, J., Miler, M.,& Matavulj, M.. (2022). Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 366-366.
https://hdl.handle.net/21.15107/rcub_ibiss_6036

Marković Filipović J, Ivelja I, Karan J, Miler M, Matavulj M. Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:366-366.
https://hdl.handle.net/21.15107/rcub_ibiss_6036 .

Marković Filipović, Jelena, Ivelja, Ivana, Karan, Jelena, Miler, Marko, Matavulj, Milica, "Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):366-366,
https://hdl.handle.net/21.15107/rcub_ibiss_6036 .

TY  - CONF
AU  - Šošić-Jurjević, Branka 
AU  - Jovanović, Ljubiša
AU  - Marina, Ljiljana
AU  - Ristić, Nataša
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Luetjohann, Dieter
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4975
AB  - The cholesterol oxidation product 27-hydroxycholesterol (27OHC) is enzymatically
produced from cholesterol by CYP27A1 in an alternative pathway of
cholesterol degradation to bile acids. This oxysterol also acts as an endogenous
selective estrogen receptor modulator (SERM). In healthy humans its
concentration in circulation increases in hypercholesterolemia and with age,
and is associated with increased risk of atherosclerosis, cardiovascular diseases
and breast cancer. Several drugs with SERM activity used for treatments of breast
cancer or osteoporosis have been reported to have sporadic hepatotoxic effects.
Women suffer from some liver diseases more commonly (acute liver failure,
autoimmune hepatitis, benign liver lesions, or primary biliary cirrhosis). For all of
these the incidence increases with advancing age. To the best of our knowledge,
there is no information in the literature, clinical or experimental, relating changes
in hepatic 27OHC with incidence of liver disease in the context of aging and sex.
To address this problem, we examined the effect of age and sex on liver and serum
concentrations of 27OHC, as well as the immunostaining pattern of CYP27A1 in
the liver of four-month and 24-month-old Wistar rats (experiments were repeated
twice with similar results, nZ5-6 animals/group) using LC MS/MS and
immunohistochemistry, respectively. Furthermore, we examined changes in
total cholesterol and concentration in liver and serum, liver histopathology, as
well as serum concentration of hepatic enzymes, alanine (ALT) and aspartate
aminotransferase (AST). The effect of age (P!0.05) on increase of serum and
hepatic 27OHC was obtained both in males and females (P!0.05) and followed
the same pattern of age-related total cholesterol increase (P!0.05). However, the
intrahepatic increase of 27OHC was dramatically more pronounced only in oldaged
females (P!0.0001). CYP27A1 immunostaining intensity was similar in all
experimental groups, being the strongest in the cytoplasm of centrilobular
hepatocytes, but the immunopositivity was diffusely spread throughout the liver
lobule. Histopathological analysis revealed age-related hepatocellular degeneration
(swelling and hydropic degeneration, increased fraction of binuclear
hepatocytes and focal fatty changes) only in females. Moreover, age-related
elevation of alanine transaminase (ALT) was observed only in female rats (P!
0.01). In conclusion, the obtained results confirmed age-related female-specific
increase of hepatic 27OHC as well as hepatocyte degeneration obsereved only in
the liver of rat females. These age-related adaptive changes in cholesterol
metabolism may atenuate hepatoprotective estrogen-like effects in the liver.
PB  - bioscientifica
C3  - 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy
T1  - Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?
DO  - 10.1530/endoabs.81.P615
SP  - P615
ER  - 

@conference{
author = "Šošić-Jurjević, Branka  and Jovanović, Ljubiša and Marina, Ljiljana and Ristić, Nataša and Miler, Marko and Ajdžanović, Vladimir and Filipović, Branko and Luetjohann, Dieter",
year = "2022",
abstract = "The cholesterol oxidation product 27-hydroxycholesterol (27OHC) is enzymatically
produced from cholesterol by CYP27A1 in an alternative pathway of
cholesterol degradation to bile acids. This oxysterol also acts as an endogenous
selective estrogen receptor modulator (SERM). In healthy humans its
concentration in circulation increases in hypercholesterolemia and with age,
and is associated with increased risk of atherosclerosis, cardiovascular diseases
and breast cancer. Several drugs with SERM activity used for treatments of breast
cancer or osteoporosis have been reported to have sporadic hepatotoxic effects.
Women suffer from some liver diseases more commonly (acute liver failure,
autoimmune hepatitis, benign liver lesions, or primary biliary cirrhosis). For all of
these the incidence increases with advancing age. To the best of our knowledge,
there is no information in the literature, clinical or experimental, relating changes
in hepatic 27OHC with incidence of liver disease in the context of aging and sex.
To address this problem, we examined the effect of age and sex on liver and serum
concentrations of 27OHC, as well as the immunostaining pattern of CYP27A1 in
the liver of four-month and 24-month-old Wistar rats (experiments were repeated
twice with similar results, nZ5-6 animals/group) using LC MS/MS and
immunohistochemistry, respectively. Furthermore, we examined changes in
total cholesterol and concentration in liver and serum, liver histopathology, as
well as serum concentration of hepatic enzymes, alanine (ALT) and aspartate
aminotransferase (AST). The effect of age (P!0.05) on increase of serum and
hepatic 27OHC was obtained both in males and females (P!0.05) and followed
the same pattern of age-related total cholesterol increase (P!0.05). However, the
intrahepatic increase of 27OHC was dramatically more pronounced only in oldaged
females (P!0.0001). CYP27A1 immunostaining intensity was similar in all
experimental groups, being the strongest in the cytoplasm of centrilobular
hepatocytes, but the immunopositivity was diffusely spread throughout the liver
lobule. Histopathological analysis revealed age-related hepatocellular degeneration
(swelling and hydropic degeneration, increased fraction of binuclear
hepatocytes and focal fatty changes) only in females. Moreover, age-related
elevation of alanine transaminase (ALT) was observed only in female rats (P!
0.01). In conclusion, the obtained results confirmed age-related female-specific
increase of hepatic 27OHC as well as hepatocyte degeneration obsereved only in
the liver of rat females. These age-related adaptive changes in cholesterol
metabolism may atenuate hepatoprotective estrogen-like effects in the liver.",
publisher = "bioscientifica",
journal = "24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy",
title = "Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?",
doi = "10.1530/endoabs.81.P615",
pages = "P615"
}

Šošić-Jurjević, B., Jovanović, L., Marina, L., Ristić, N., Miler, M., Ajdžanović, V., Filipović, B.,& Luetjohann, D.. (2022). Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?. in 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy
bioscientifica., P615.
https://doi.org/10.1530/endoabs.81.P615

Šošić-Jurjević B, Jovanović L, Marina L, Ristić N, Miler M, Ajdžanović V, Filipović B, Luetjohann D. Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?. in 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy. 2022;:P615.
doi:10.1530/endoabs.81.P615 .

Šošić-Jurjević, Branka , Jovanović, Ljubiša, Marina, Ljiljana, Ristić, Nataša, Miler, Marko, Ajdžanović, Vladimir, Filipović, Branko, Luetjohann, Dieter, "Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?" in 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy (2022):P615,
https://doi.org/10.1530/endoabs.81.P615 . .

TY  - JOUR
AU  - Ruskovska, Tatjana
AU  - Budić-Leto, Irena
AU  - Corral-Jara, Karla Fabiola
AU  - Ajdžanović, Vladimir
AU  - Arola-Arnal, Anna
AU  - Bravo, Francisca Isabel
AU  - Deligiannidou, Georgia-Eirini
AU  - Havlik, Jaroslav
AU  - Janeva, Milkica
AU  - Kistanova, Elena
AU  - Kontogiorgis, Christos
AU  - Krga, Irena
AU  - Massaro, Marika
AU  - Miler, Marko
AU  - Harnafi, Hicham
AU  - Milošević, Verica
AU  - Morand, Christine
AU  - Scoditti, Egeria
AU  - Suárez, Manuel
AU  - Vauzour, David
AU  - Milenkovic, Dragan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4977
AB  - Cardiovascular and metabolic disorders present major causes of mortality in the ageing population. Polyphenols present in human diets possess cardiometabolic protective properties, however their underlying molecular mechanisms in humans are still not well identified. Even though preclinical and in vitro studies advocate that these bioactives can modulate gene expression, most studies were performed using targeted approaches. With the objective to decipher the molecular mechanisms underlying polyphenols cardiometabolic preventive properties in humans, we performed integrative multi-omic bioinformatic analyses of published studies which reported improvements of cardiometabolic risk factors following polyphenol intake, together with genomic analyses performed using untargeted approach. We identified 5 studies within our criteria and nearly 5000 differentially expressed genes, both mRNAs and miRNAs, in peripheral blood cells. Integrative bioinformatic analyses (e.g. pathway and gene network analyses, identification of transcription factors, correlation of gene expression profiles with those associated with diseases and drug intake) revealed that these genes are involved in the processes such as cell adhesion and mobility, immune system, metabolism, or cell signaling. We also identified 27 miRNAs known to regulate processes such as cell cytoskeleton, chemotaxis, cell signaling, or cell metabolism. Gene expression profiles negatively correlated with expression profiles of cardiovascular disease patients, while a positive correlation was observed with gene expression profiles following intake of drugs against cardiometabolic disorders. These analyses further advocate for health protective effects of these bioactives against age-associated diseases. In conclusion, polyphenols can exert multi-genomic modifications in humans and use of untargeted methods coupled with bioinformatic analyses represent the best approach to decipher molecular mechanisms underlying healthy-ageing effects of these bioactives.
PB  - Elsevier B.V.
T2  - Ageing Research Reviews
T1  - Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies
VL  - 79:101649
DO  - 10.1016/j.arr.2022.101649
ER  - 

@article{
author = "Ruskovska, Tatjana and Budić-Leto, Irena and Corral-Jara, Karla Fabiola and Ajdžanović, Vladimir and Arola-Arnal, Anna and Bravo, Francisca Isabel and Deligiannidou, Georgia-Eirini and Havlik, Jaroslav and Janeva, Milkica and Kistanova, Elena and Kontogiorgis, Christos and Krga, Irena and Massaro, Marika and Miler, Marko and Harnafi, Hicham and Milošević, Verica and Morand, Christine and Scoditti, Egeria and Suárez, Manuel and Vauzour, David and Milenkovic, Dragan",
year = "2022",
abstract = "Cardiovascular and metabolic disorders present major causes of mortality in the ageing population. Polyphenols present in human diets possess cardiometabolic protective properties, however their underlying molecular mechanisms in humans are still not well identified. Even though preclinical and in vitro studies advocate that these bioactives can modulate gene expression, most studies were performed using targeted approaches. With the objective to decipher the molecular mechanisms underlying polyphenols cardiometabolic preventive properties in humans, we performed integrative multi-omic bioinformatic analyses of published studies which reported improvements of cardiometabolic risk factors following polyphenol intake, together with genomic analyses performed using untargeted approach. We identified 5 studies within our criteria and nearly 5000 differentially expressed genes, both mRNAs and miRNAs, in peripheral blood cells. Integrative bioinformatic analyses (e.g. pathway and gene network analyses, identification of transcription factors, correlation of gene expression profiles with those associated with diseases and drug intake) revealed that these genes are involved in the processes such as cell adhesion and mobility, immune system, metabolism, or cell signaling. We also identified 27 miRNAs known to regulate processes such as cell cytoskeleton, chemotaxis, cell signaling, or cell metabolism. Gene expression profiles negatively correlated with expression profiles of cardiovascular disease patients, while a positive correlation was observed with gene expression profiles following intake of drugs against cardiometabolic disorders. These analyses further advocate for health protective effects of these bioactives against age-associated diseases. In conclusion, polyphenols can exert multi-genomic modifications in humans and use of untargeted methods coupled with bioinformatic analyses represent the best approach to decipher molecular mechanisms underlying healthy-ageing effects of these bioactives.",
publisher = "Elsevier B.V.",
journal = "Ageing Research Reviews",
title = "Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies",
volume = "79:101649",
doi = "10.1016/j.arr.2022.101649"
}

Ruskovska, T., Budić-Leto, I., Corral-Jara, K. F., Ajdžanović, V., Arola-Arnal, A., Bravo, F. I., Deligiannidou, G., Havlik, J., Janeva, M., Kistanova, E., Kontogiorgis, C., Krga, I., Massaro, M., Miler, M., Harnafi, H., Milošević, V., Morand, C., Scoditti, E., Suárez, M., Vauzour, D.,& Milenkovic, D.. (2022). Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies. in Ageing Research Reviews
Elsevier B.V.., 79:101649.
https://doi.org/10.1016/j.arr.2022.101649

Ruskovska T, Budić-Leto I, Corral-Jara KF, Ajdžanović V, Arola-Arnal A, Bravo FI, Deligiannidou G, Havlik J, Janeva M, Kistanova E, Kontogiorgis C, Krga I, Massaro M, Miler M, Harnafi H, Milošević V, Morand C, Scoditti E, Suárez M, Vauzour D, Milenkovic D. Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies. in Ageing Research Reviews. 2022;79:101649.
doi:10.1016/j.arr.2022.101649 .

Ruskovska, Tatjana, Budić-Leto, Irena, Corral-Jara, Karla Fabiola, Ajdžanović, Vladimir, Arola-Arnal, Anna, Bravo, Francisca Isabel, Deligiannidou, Georgia-Eirini, Havlik, Jaroslav, Janeva, Milkica, Kistanova, Elena, Kontogiorgis, Christos, Krga, Irena, Massaro, Marika, Miler, Marko, Harnafi, Hicham, Milošević, Verica, Morand, Christine, Scoditti, Egeria, Suárez, Manuel, Vauzour, David, Milenkovic, Dragan, "Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies" in Ageing Research Reviews, 79:101649 (2022),
https://doi.org/10.1016/j.arr.2022.101649 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milenković, Dragan
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4144
AB  - In a series of our previous works, we revealed the beneficial effects of applied soy isoflavones (genistein or
daidzein) on the wide context of corticosteroidogenesis in vivo, in a rat model of the andropause. Soy isoflavones
decreased the circulating levels of pituitary adrenocorticotropic hormone, inhibited aldosterone secretion, as
well as corticosterone production and secretion, but stimulated dehydroepiandrosterone secretion, all in
andropausal rats. In vitro studies indicate that the mechanism underlying these hormonal changes relies on inhibition of the pituitary tyrosine kinase and adrenocortical 3β-hydroxysteroid dehydrogenase enzymes by soy
isoflavones. Although the clinical studies are in their infancy, the opinion is that genistein and daidzein have
therapeutic potential for the safe treatment of ageing-caused androgen deprivation and glucocorticoid excess
with related metabolic/hemodynamic issues in males. Our accumulated experience and knowledge in the field of
biomedical effects of plant polyphenols have provided a platform for potential recommending the agenda to
organize and accelerate experimental research aimed at producing the optimal supplementation. We hypothesize
that an in vivo approach should first be exploited in the sequence of investigative steps, followed by in vitro
studies and synchronously conducted molecular docking analyses. In vivo research, besides establishing the
margin of exposure safety or adjustment of the correct polyphenol dose, enables identification and quantification
of the metabolites of applied polyphenols in the blood. Subsequent in vitro exploitation of the metabolites and
related docking analyses provide clarification of the molecular mechanisms of action of applied polyphenols.
Chemical modification of the polyphenol structure or coupling it with nanoparticles might be the next step in
optimizing the design of supplementation. Selected, intact or chemically-modified polyphenol molecules should
be included in preclinical studies on a more closely-related species, while clinical studies would finally assess the
safety and effectiveness of a polyphenol-based remedial strategy. The final supplement represents a product of an
appropriate technological process, conducted in accordance with the recommendations derived from the preceding research.
PB  - Elsevir
T2  - Medical Hypotheses
T1  - Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design
VL  - 148
DO  - 10.1016/j.mehy.2021.110516
SP  - 110516
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka  and Filipović, Branko and Milenković, Dragan and Jakovljević, Vladimir and Milošević, Verica",
year = "2021",
abstract = "In a series of our previous works, we revealed the beneficial effects of applied soy isoflavones (genistein or
daidzein) on the wide context of corticosteroidogenesis in vivo, in a rat model of the andropause. Soy isoflavones
decreased the circulating levels of pituitary adrenocorticotropic hormone, inhibited aldosterone secretion, as
well as corticosterone production and secretion, but stimulated dehydroepiandrosterone secretion, all in
andropausal rats. In vitro studies indicate that the mechanism underlying these hormonal changes relies on inhibition of the pituitary tyrosine kinase and adrenocortical 3β-hydroxysteroid dehydrogenase enzymes by soy
isoflavones. Although the clinical studies are in their infancy, the opinion is that genistein and daidzein have
therapeutic potential for the safe treatment of ageing-caused androgen deprivation and glucocorticoid excess
with related metabolic/hemodynamic issues in males. Our accumulated experience and knowledge in the field of
biomedical effects of plant polyphenols have provided a platform for potential recommending the agenda to
organize and accelerate experimental research aimed at producing the optimal supplementation. We hypothesize
that an in vivo approach should first be exploited in the sequence of investigative steps, followed by in vitro
studies and synchronously conducted molecular docking analyses. In vivo research, besides establishing the
margin of exposure safety or adjustment of the correct polyphenol dose, enables identification and quantification
of the metabolites of applied polyphenols in the blood. Subsequent in vitro exploitation of the metabolites and
related docking analyses provide clarification of the molecular mechanisms of action of applied polyphenols.
Chemical modification of the polyphenol structure or coupling it with nanoparticles might be the next step in
optimizing the design of supplementation. Selected, intact or chemically-modified polyphenol molecules should
be included in preclinical studies on a more closely-related species, while clinical studies would finally assess the
safety and effectiveness of a polyphenol-based remedial strategy. The final supplement represents a product of an
appropriate technological process, conducted in accordance with the recommendations derived from the preceding research.",
publisher = "Elsevir",
journal = "Medical Hypotheses",
title = "Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design",
volume = "148",
doi = "10.1016/j.mehy.2021.110516",
pages = "110516"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B., Filipović, B., Milenković, D., Jakovljević, V.,& Milošević, V.. (2021). Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design. in Medical Hypotheses
Elsevir., 148, 110516.
https://doi.org/10.1016/j.mehy.2021.110516

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B, Milenković D, Jakovljević V, Milošević V. Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design. in Medical Hypotheses. 2021;148:110516.
doi:10.1016/j.mehy.2021.110516 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka , Filipović, Branko, Milenković, Dragan, Jakovljević, Vladimir, Milošević, Verica, "Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design" in Medical Hypotheses, 148 (2021):110516,
https://doi.org/10.1016/j.mehy.2021.110516 . .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Ivelja, Ivana
AU  - Karan, Jelena
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Matavulj, Milica
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6577
AB  - INTRODUCTION: Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory purposes. In addition, AA is formed in many types of fried and oven-baked foods during cooking. Considering proven neurotoxic, carcinogenic and mutagenic effects on living organisms, AA became a main topic of interest for many research groups. OBJECTIVES: The objective of our study was to determine whether acrylamide treatment affects superoxide dismutase 1 (SOD1) expression in rat liver. METHOD / DESIGN: Adult male Wistar rats were subchronicly (three weeks) treated with 25 mg/kg or 50 mg/kg body weight (b.w.) of AA. Formalin-fixed paraffin-embedded liver tissue was cut into 5 µm thin sections and immunostained with anti-SOD1 antibody. The amount of SOD1 in immunostained sections was determined using Windows based ImageJ program (ImageJ, Version 1.50f). The optical density (OD) and stained percentage color area of immunolabeled SOD1 were measured. RESULTS: Figure 1. Representative micrographs of superoxide dismutase 1 (SOD1) immunohistochemical staining in liver of control rats, rats treated with
acrylamide (AA) in dose of 25 mg/kg b.w., and rats treated with acrylamide in dose of 50 mg/kg b.w. (a). Optical density of SOD1 immunopositive cells in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (b). Percentage contribution of high positive, positive, low positive and total positive immunohistochemical staining of SOD1 in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (c). Values in charts are means ± SEM; n = 10, *p < 0.05. In statistical analysis AA-treated animals were compared with the control group. CONCLUSIONS: Immunostaining of SOD1 in liver of control rats showed weak cytoplasmic immunoreactivity in hepatocytes (Fig. 1a). AA application induced dose dependent increase of immunostaining intensity (Fig. 1a). Significant increase of OD and percentage contribution of low positive and total positive cells of immunostained SOD1 was detected in group treated with AA in a dose of 50 mg/kg (Fig.1b, c).
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver
SP  - 137
EP  - 138
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6577
ER  - 

@conference{
author = "Marković Filipović, Jelena and Ivelja, Ivana and Karan, Jelena and Miler, Marko and Milošević, Verica and Matavulj, Milica",
year = "2021",
abstract = "INTRODUCTION: Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory purposes. In addition, AA is formed in many types of fried and oven-baked foods during cooking. Considering proven neurotoxic, carcinogenic and mutagenic effects on living organisms, AA became a main topic of interest for many research groups. OBJECTIVES: The objective of our study was to determine whether acrylamide treatment affects superoxide dismutase 1 (SOD1) expression in rat liver. METHOD / DESIGN: Adult male Wistar rats were subchronicly (three weeks) treated with 25 mg/kg or 50 mg/kg body weight (b.w.) of AA. Formalin-fixed paraffin-embedded liver tissue was cut into 5 µm thin sections and immunostained with anti-SOD1 antibody. The amount of SOD1 in immunostained sections was determined using Windows based ImageJ program (ImageJ, Version 1.50f). The optical density (OD) and stained percentage color area of immunolabeled SOD1 were measured. RESULTS: Figure 1. Representative micrographs of superoxide dismutase 1 (SOD1) immunohistochemical staining in liver of control rats, rats treated with
acrylamide (AA) in dose of 25 mg/kg b.w., and rats treated with acrylamide in dose of 50 mg/kg b.w. (a). Optical density of SOD1 immunopositive cells in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (b). Percentage contribution of high positive, positive, low positive and total positive immunohistochemical staining of SOD1 in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (c). Values in charts are means ± SEM; n = 10, *p < 0.05. In statistical analysis AA-treated animals were compared with the control group. CONCLUSIONS: Immunostaining of SOD1 in liver of control rats showed weak cytoplasmic immunoreactivity in hepatocytes (Fig. 1a). AA application induced dose dependent increase of immunostaining intensity (Fig. 1a). Significant increase of OD and percentage contribution of low positive and total positive cells of immunostained SOD1 was detected in group treated with AA in a dose of 50 mg/kg (Fig.1b, c).",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver",
pages = "137-138",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6577"
}

Marković Filipović, J., Ivelja, I., Karan, J., Miler, M., Milošević, V.,& Matavulj, M.. (2021). Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 137-138.
https://hdl.handle.net/21.15107/rcub_ibiss_6577

Marković Filipović J, Ivelja I, Karan J, Miler M, Milošević V, Matavulj M. Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:137-138.
https://hdl.handle.net/21.15107/rcub_ibiss_6577 .

Marković Filipović, Jelena, Ivelja, Ivana, Karan, Jelena, Miler, Marko, Milošević, Verica, Matavulj, Milica, "Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):137-138,
https://hdl.handle.net/21.15107/rcub_ibiss_6577 .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Kojić, Danijela
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Matavulj, Milica
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6578
AB  - INTRODUCTION: Acrylamide (AA) is industrial toxic substance with neurotoxic and reprotoxic effects. AA is a Maillard reaction product formed during processing of starchy food at high temperature.
OBJECTIVES: The objective of our study was to determine whether acrylamide treatment disturbs redox balance by altering nitrite, gluthatione (GSH), and malondialdehyde levels in rat hepatoma cell line (H4IIE). METHOD / DESIGN:
Rat hepatoma cell line H4IIE was treated with 4 mM (IC20) and 4.5 mM (IC50) of AA for 24 h. The nitrite level in the medium was analyzed as an indicator of NO production following the Griess reaction method. After ultrasonic cell lysis in 2.5% sulfocalicylic acid, supernatant was analysed for the content of gluthatione. Lipid peroxidation was evaluated using thiobarbituric acid reactive substance assay (TBARS). RESULTS: Detected nitrite, malondialdehyde and GSH levels in rat hepatoma cell line H4IIE after acrylamide treatment are shown in Figure 1. Figure 1. Nitrite concentration (a), malondialdehyde (MDA) concentration (b), reduced glutathione (GSH) concentration (c) in H4IIE cells after treatment with 4 and 4.5 mM acrylamide (AA) for 24 h. Values in charts are means ± SEM of three experiments performed in triplicate. Mean values were significantly different from that of untreated control cells (*p<0.05). CONCLUSIONS: In rat hepatoma cell line H4IIE, exposure to AA caused significant concentration-dependent increase of nitrite level and lipid peroxidation (Fig. 1a, b). On the other hand, GSH content significantly decreased in a concentration-dependent manner in H4IIE cells (Fig.1c). Obtained results indicate that AA disturbs redox status in hepatocytes.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Acrylamide treatment affects oxidative stress parameters in rat hepatocytes
SP  - 139
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6578
ER  - 

@conference{
author = "Marković Filipović, Jelena and Kojić, Danijela and Miler, Marko and Milošević, Verica and Matavulj, Milica",
year = "2021",
abstract = "INTRODUCTION: Acrylamide (AA) is industrial toxic substance with neurotoxic and reprotoxic effects. AA is a Maillard reaction product formed during processing of starchy food at high temperature.
OBJECTIVES: The objective of our study was to determine whether acrylamide treatment disturbs redox balance by altering nitrite, gluthatione (GSH), and malondialdehyde levels in rat hepatoma cell line (H4IIE). METHOD / DESIGN:
Rat hepatoma cell line H4IIE was treated with 4 mM (IC20) and 4.5 mM (IC50) of AA for 24 h. The nitrite level in the medium was analyzed as an indicator of NO production following the Griess reaction method. After ultrasonic cell lysis in 2.5% sulfocalicylic acid, supernatant was analysed for the content of gluthatione. Lipid peroxidation was evaluated using thiobarbituric acid reactive substance assay (TBARS). RESULTS: Detected nitrite, malondialdehyde and GSH levels in rat hepatoma cell line H4IIE after acrylamide treatment are shown in Figure 1. Figure 1. Nitrite concentration (a), malondialdehyde (MDA) concentration (b), reduced glutathione (GSH) concentration (c) in H4IIE cells after treatment with 4 and 4.5 mM acrylamide (AA) for 24 h. Values in charts are means ± SEM of three experiments performed in triplicate. Mean values were significantly different from that of untreated control cells (*p<0.05). CONCLUSIONS: In rat hepatoma cell line H4IIE, exposure to AA caused significant concentration-dependent increase of nitrite level and lipid peroxidation (Fig. 1a, b). On the other hand, GSH content significantly decreased in a concentration-dependent manner in H4IIE cells (Fig.1c). Obtained results indicate that AA disturbs redox status in hepatocytes.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Acrylamide treatment affects oxidative stress parameters in rat hepatocytes",
pages = "139-140",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6578"
}

Marković Filipović, J., Kojić, D., Miler, M., Milošević, V.,& Matavulj, M.. (2021). Acrylamide treatment affects oxidative stress parameters in rat hepatocytes. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6578

Marković Filipović J, Kojić D, Miler M, Milošević V, Matavulj M. Acrylamide treatment affects oxidative stress parameters in rat hepatocytes. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6578 .

Marković Filipović, Jelena, Kojić, Danijela, Miler, Marko, Milošević, Verica, Matavulj, Milica, "Acrylamide treatment affects oxidative stress parameters in rat hepatocytes" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):139-140,
https://hdl.handle.net/21.15107/rcub_ibiss_6578 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Miler, Marko
AU  - Milošević, Verica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4223
PB  - Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors
T2  - EXCLI Journal
T1  - Margins of beneficial daily dosage of supplements in prevention of COVID-19
VL  - 20
DO  - 10.17179/excli2021-3790
SP  - 828
EP  - 834
ER  - 

@article{
author = "Ajdžanović, Vladimir and Filipović, Branko and Šošić-Jurjević, Branka  and Miler, Marko and Milošević, Verica",
year = "2021",
publisher = "Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors",
journal = "EXCLI Journal",
title = "Margins of beneficial daily dosage of supplements in prevention of COVID-19",
volume = "20",
doi = "10.17179/excli2021-3790",
pages = "828-834"
}

Ajdžanović, V., Filipović, B., Šošić-Jurjević, B., Miler, M.,& Milošević, V.. (2021). Margins of beneficial daily dosage of supplements in prevention of COVID-19. in EXCLI Journal
Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors., 20, 828-834.
https://doi.org/10.17179/excli2021-3790

Ajdžanović V, Filipović B, Šošić-Jurjević B, Miler M, Milošević V. Margins of beneficial daily dosage of supplements in prevention of COVID-19. in EXCLI Journal. 2021;20:828-834.
doi:10.17179/excli2021-3790 .

Ajdžanović, Vladimir, Filipović, Branko, Šošić-Jurjević, Branka , Miler, Marko, Milošević, Verica, "Margins of beneficial daily dosage of supplements in prevention of COVID-19" in EXCLI Journal, 20 (2021):828-834,
https://doi.org/10.17179/excli2021-3790 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
PY  - 2021
UR  - https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/abs/thyroid-gland-alterations-in-oldaged-wistar-rats-a-comprehensive-stereological-ultrastructural-hormonal-and-gene-expression-study/4C35B9A6AFED404C65272C08C8C2DC27
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4143
AB  - The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using
stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation,
presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute
volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes
of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin
(Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial
growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles.
Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene
expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF
increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression
of the thyroid gland synthetic and secretory capacity with advanced age.
PB  - Cambridge University Press
T2  - Microscopy and Microanalysis
T1  - Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study
DO  - 10.1017/S1431927621000064
ER  - 

@article{
author = "Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Marković Filipović, Jelena and Šošić-Jurjević, Branka  and Milošević, Verica",
year = "2021",
abstract = "The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using
stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation,
presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute
volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes
of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin
(Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial
growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles.
Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene
expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF
increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression
of the thyroid gland synthetic and secretory capacity with advanced age.",
publisher = "Cambridge University Press",
journal = "Microscopy and Microanalysis",
title = "Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study",
doi = "10.1017/S1431927621000064"
}

Miler, M., Ajdžanović, V., Živanović, J., Marković Filipović, J., Šošić-Jurjević, B.,& Milošević, V.. (2021). Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. in Microscopy and Microanalysis
Cambridge University Press..
https://doi.org/10.1017/S1431927621000064

Miler M, Ajdžanović V, Živanović J, Marković Filipović J, Šošić-Jurjević B, Milošević V. Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. in Microscopy and Microanalysis. 2021;.
doi:10.1017/S1431927621000064 .

Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Marković Filipović, Jelena, Šošić-Jurjević, Branka , Milošević, Verica, "Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study" in Microscopy and Microanalysis (2021),
https://doi.org/10.1017/S1431927621000064 . .

TY  - JOUR
AU  - Platanić Arizanović, Lena
AU  - Nikolić-Kokić, Aleksandra
AU  - Brkljačić, Jelena
AU  - Tatalović, Nikola
AU  - Miler, Marko
AU  - Oreščanin Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić, Milan
AU  - Milošević, Verica
AU  - Blagojević, Duško
AU  - Spasić, Snežana
AU  - Miljević, Čedo
PY  - 2021
UR  - https://www.tandfonline.com/doi/abs/10.1080/15287394.2020.1844827
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4037
AB  - Chronic use of atypical antipsychotics may produce hepatic damage. Atypical antipsychotics, including clozapine, sertindole, and ziprasidone, are extensively metabolized by the liver and this process generates toxic-free radical metabolic intermediates which may contribute to liver damage. The aim of this study was to investigate whether clozapine, sertindole, or ziprasidone affected hepatic antioxidant defense enzymes which consequently led to disturbed redox homeostasis. The expression and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferases (GST) were measured in rat livers at doses corresponding to human antipsychotic therapy. Clozapine increased activity of SOD types 1 and 2, GR and GST, but reduced CAT activity. Sertindole elevated activities of both SODs. In ziprasidone-treated rats only decreased CAT activity was found. All three antipsychotics produced mild-to-moderate hepatic histopathological changes categorized as regenerative alterations. No apparent signs of immune cell infiltration, microvesicular or macrovesicular fatty change, or hepatocytes in mitosis were observed. In conclusion, a 4-week long daily treatment with clozapine, sertindole, or ziprasidone altered hepatic antioxidant enzyme activities and induced histopathological changes in liver. The most severe alterations were noted in clozapine-treated rats. Data indicate that redox disturbances may contribute to liver dysfunction after long-term atypical antipsychotic drug treatment.
PB  - Bellwether Publishing, Ltd.
T2  - Journal of Toxicology and Environmental Health, Part A
T1  - Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction
IS  - 4
VL  - 84
DO  - 10.1080/15287394.2020.1844827
SP  - 173
EP  - 182
ER  - 

@article{
author = "Platanić Arizanović, Lena and Nikolić-Kokić, Aleksandra and Brkljačić, Jelena and Tatalović, Nikola and Miler, Marko and Oreščanin Dušić, Zorana and Vidonja Uzelac, Teodora and Nikolić, Milan and Milošević, Verica and Blagojević, Duško and Spasić, Snežana and Miljević, Čedo",
year = "2021",
abstract = "Chronic use of atypical antipsychotics may produce hepatic damage. Atypical antipsychotics, including clozapine, sertindole, and ziprasidone, are extensively metabolized by the liver and this process generates toxic-free radical metabolic intermediates which may contribute to liver damage. The aim of this study was to investigate whether clozapine, sertindole, or ziprasidone affected hepatic antioxidant defense enzymes which consequently led to disturbed redox homeostasis. The expression and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferases (GST) were measured in rat livers at doses corresponding to human antipsychotic therapy. Clozapine increased activity of SOD types 1 and 2, GR and GST, but reduced CAT activity. Sertindole elevated activities of both SODs. In ziprasidone-treated rats only decreased CAT activity was found. All three antipsychotics produced mild-to-moderate hepatic histopathological changes categorized as regenerative alterations. No apparent signs of immune cell infiltration, microvesicular or macrovesicular fatty change, or hepatocytes in mitosis were observed. In conclusion, a 4-week long daily treatment with clozapine, sertindole, or ziprasidone altered hepatic antioxidant enzyme activities and induced histopathological changes in liver. The most severe alterations were noted in clozapine-treated rats. Data indicate that redox disturbances may contribute to liver dysfunction after long-term atypical antipsychotic drug treatment.",
publisher = "Bellwether Publishing, Ltd.",
journal = "Journal of Toxicology and Environmental Health, Part A",
title = "Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction",
number = "4",
volume = "84",
doi = "10.1080/15287394.2020.1844827",
pages = "173-182"
}

Platanić Arizanović, L., Nikolić-Kokić, A., Brkljačić, J., Tatalović, N., Miler, M., Oreščanin Dušić, Z., Vidonja Uzelac, T., Nikolić, M., Milošević, V., Blagojević, D., Spasić, S.,& Miljević, Č.. (2021). Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction. in Journal of Toxicology and Environmental Health, Part A
Bellwether Publishing, Ltd.., 84(4), 173-182.
https://doi.org/10.1080/15287394.2020.1844827

Platanić Arizanović L, Nikolić-Kokić A, Brkljačić J, Tatalović N, Miler M, Oreščanin Dušić Z, Vidonja Uzelac T, Nikolić M, Milošević V, Blagojević D, Spasić S, Miljević Č. Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction. in Journal of Toxicology and Environmental Health, Part A. 2021;84(4):173-182.
doi:10.1080/15287394.2020.1844827 .

Platanić Arizanović, Lena, Nikolić-Kokić, Aleksandra, Brkljačić, Jelena, Tatalović, Nikola, Miler, Marko, Oreščanin Dušić, Zorana, Vidonja Uzelac, Teodora, Nikolić, Milan, Milošević, Verica, Blagojević, Duško, Spasić, Snežana, Miljević, Čedo, "Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction" in Journal of Toxicology and Environmental Health, Part A, 84, no. 4 (2021):173-182,
https://doi.org/10.1080/15287394.2020.1844827 . .

TY  - JOUR
AU  - Ruskovska, Tatjana
AU  - Budić-Leto, Irena
AU  - Corral-Jara, Karla Fabiola
AU  - Ajdžanović, Vladimir
AU  - Arola-Arnal, Anna
AU  - Bravo, Francisca Isabel
AU  - Deligiannidou, Georgia-Eirini
AU  - Havlik, Jaroslav
AU  - Janeva, Milkica
AU  - Kistanova, Elena
AU  - Kontogiorgis, Christos
AU  - Krga, Irena
AU  - Massaro, Marika
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Morand, Christine
AU  - Scoditti, Egeria
AU  - Suárez, Manuel
AU  - Vauzour, David
AU  - Milenković, Dragan
PY  - 2021
UR  - https://www.mdpi.com/2072-6643/13/7/2326
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4435
AB  - Cardiometabolic disorders are among the leading causes of mortality in the human population. Dietary polyphenols exert beneficial effects on cardiometabolic health in humans. Molecular mechanisms, however, are not completely understood. Aiming to conduct in-depth integrative bioinformatic analyses to elucidate molecular mechanisms underlying the protective effects of polyphenols on cardiometabolic health, we first conducted a systematic literature search to identify human intervention studies with polyphenols that demonstrate improvement of cardiometabolic risk factors in parallel with significant nutrigenomic effects. Applying the predefined inclusion criteria, we identified 58 differentially expressed genes at mRNA level and 5 miRNAs, analyzed in peripheral blood cells with RT-PCR methods. Subsequent integrative bioinformatic analyses demonstrated that polyphenols modulate genes that are mainly involved in the processes such as inflammation, lipid metabolism, and endothelial function. We also identified 37 transcription factors that are involved in the regulation of polyphenol modulated genes, including RELA/NFKB1, STAT1, JUN, or SIRT1. Integrative bioinformatic analysis of mRNA and miRNA-target pathways demonstrated several common enriched pathways that include MAPK signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, focal adhesion, or PPAR signaling pathway. These bioinformatic analyses represent a valuable source of information for the identification of molecular mechanisms underlying the beneficial health effects of polyphenols and potential target genes for future nutrigenetic studies.
PB  - Basel: MDPI
T2  - Nutrients
T1  - Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans-Evidence from Targeted Nutrigenomic Studies.
IS  - 7
VL  - 13
DO  - 10.3390/nu13072326
SP  - 2326
ER  - 

@article{
author = "Ruskovska, Tatjana and Budić-Leto, Irena and Corral-Jara, Karla Fabiola and Ajdžanović, Vladimir and Arola-Arnal, Anna and Bravo, Francisca Isabel and Deligiannidou, Georgia-Eirini and Havlik, Jaroslav and Janeva, Milkica and Kistanova, Elena and Kontogiorgis, Christos and Krga, Irena and Massaro, Marika and Miler, Marko and Milošević, Verica and Morand, Christine and Scoditti, Egeria and Suárez, Manuel and Vauzour, David and Milenković, Dragan",
year = "2021",
abstract = "Cardiometabolic disorders are among the leading causes of mortality in the human population. Dietary polyphenols exert beneficial effects on cardiometabolic health in humans. Molecular mechanisms, however, are not completely understood. Aiming to conduct in-depth integrative bioinformatic analyses to elucidate molecular mechanisms underlying the protective effects of polyphenols on cardiometabolic health, we first conducted a systematic literature search to identify human intervention studies with polyphenols that demonstrate improvement of cardiometabolic risk factors in parallel with significant nutrigenomic effects. Applying the predefined inclusion criteria, we identified 58 differentially expressed genes at mRNA level and 5 miRNAs, analyzed in peripheral blood cells with RT-PCR methods. Subsequent integrative bioinformatic analyses demonstrated that polyphenols modulate genes that are mainly involved in the processes such as inflammation, lipid metabolism, and endothelial function. We also identified 37 transcription factors that are involved in the regulation of polyphenol modulated genes, including RELA/NFKB1, STAT1, JUN, or SIRT1. Integrative bioinformatic analysis of mRNA and miRNA-target pathways demonstrated several common enriched pathways that include MAPK signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, focal adhesion, or PPAR signaling pathway. These bioinformatic analyses represent a valuable source of information for the identification of molecular mechanisms underlying the beneficial health effects of polyphenols and potential target genes for future nutrigenetic studies.",
publisher = "Basel: MDPI",
journal = "Nutrients",
title = "Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans-Evidence from Targeted Nutrigenomic Studies.",
number = "7",
volume = "13",
doi = "10.3390/nu13072326",
pages = "2326"
}

Ruskovska, T., Budić-Leto, I., Corral-Jara, K. F., Ajdžanović, V., Arola-Arnal, A., Bravo, F. I., Deligiannidou, G., Havlik, J., Janeva, M., Kistanova, E., Kontogiorgis, C., Krga, I., Massaro, M., Miler, M., Milošević, V., Morand, C., Scoditti, E., Suárez, M., Vauzour, D.,& Milenković, D.. (2021). Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans-Evidence from Targeted Nutrigenomic Studies.. in Nutrients
Basel: MDPI., 13(7), 2326.
https://doi.org/10.3390/nu13072326

Ruskovska T, Budić-Leto I, Corral-Jara KF, Ajdžanović V, Arola-Arnal A, Bravo FI, Deligiannidou G, Havlik J, Janeva M, Kistanova E, Kontogiorgis C, Krga I, Massaro M, Miler M, Milošević V, Morand C, Scoditti E, Suárez M, Vauzour D, Milenković D. Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans-Evidence from Targeted Nutrigenomic Studies.. in Nutrients. 2021;13(7):2326.
doi:10.3390/nu13072326 .

Ruskovska, Tatjana, Budić-Leto, Irena, Corral-Jara, Karla Fabiola, Ajdžanović, Vladimir, Arola-Arnal, Anna, Bravo, Francisca Isabel, Deligiannidou, Georgia-Eirini, Havlik, Jaroslav, Janeva, Milkica, Kistanova, Elena, Kontogiorgis, Christos, Krga, Irena, Massaro, Marika, Miler, Marko, Milošević, Verica, Morand, Christine, Scoditti, Egeria, Suárez, Manuel, Vauzour, David, Milenković, Dragan, "Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans-Evidence from Targeted Nutrigenomic Studies." in Nutrients, 13, no. 7 (2021):2326,
https://doi.org/10.3390/nu13072326 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Popovska-Perčinić, Florina
AU  - Milošević, Verica
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32120001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3627
AB  - INTRODUCTION AND AIM Daidzein application may represent an effective and less harmful alternative to indicated, classical estrogenization of ageing men. The aim of this study was to perform structural and hormonal analysis of the adrenal cortex, after estradiol or daidzein supplementation in a rat model of the andropause. MATERIAL AND METHODS Middle-aged Wistar rats were divided into sham operated (SO; n = 8), orchidectomized (Orx; n = 8), estradiol treated orchidectomized (Orx + E; n = 8) and daidzein treated orchidectomized (Orx + D; n = 8) groups. Estradiol (0.625 mg/kg b.m./day) or daidzein (30 mg/kg b.m./day) were administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis. RESULTS Both estradiol and daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei, but decreased circulating aldosterone levels. Estradiol markedly increased volumes of the zona fasciculata cell and nuclei in parallel with significant decrease of the adrenal tissue level of corticosterone, while daidzein significantly decreased both the adrenal and circulating levels of corticosterone. Serum DHEA level and volumes of the zona reticularis cell and nuclei significantly increased upon estradiol treatment, whereas daidzein even stronger increased the circulating level of DHEA. Shunting of the corticosteroidogenesis pathways towards adrenal androgens production, after the treatments, corresponded to the ultrastructural findings and zonal capillary network rearrangements. CONCLUSIONS Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of ageing-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.
T2  - Annals of Anatomy
T1  - The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.
VL  - 230
DO  - 10.1016/j.aanat.2020.151487
SP  - 151487
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Živanović, Jasmina and Filipović, Branko and Šošić-Jurjević, Branka  and Popovska-Perčinić, Florina and Milošević, Verica",
year = "2020",
abstract = "INTRODUCTION AND AIM Daidzein application may represent an effective and less harmful alternative to indicated, classical estrogenization of ageing men. The aim of this study was to perform structural and hormonal analysis of the adrenal cortex, after estradiol or daidzein supplementation in a rat model of the andropause. MATERIAL AND METHODS Middle-aged Wistar rats were divided into sham operated (SO; n = 8), orchidectomized (Orx; n = 8), estradiol treated orchidectomized (Orx + E; n = 8) and daidzein treated orchidectomized (Orx + D; n = 8) groups. Estradiol (0.625 mg/kg b.m./day) or daidzein (30 mg/kg b.m./day) were administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis. RESULTS Both estradiol and daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei, but decreased circulating aldosterone levels. Estradiol markedly increased volumes of the zona fasciculata cell and nuclei in parallel with significant decrease of the adrenal tissue level of corticosterone, while daidzein significantly decreased both the adrenal and circulating levels of corticosterone. Serum DHEA level and volumes of the zona reticularis cell and nuclei significantly increased upon estradiol treatment, whereas daidzein even stronger increased the circulating level of DHEA. Shunting of the corticosteroidogenesis pathways towards adrenal androgens production, after the treatments, corresponded to the ultrastructural findings and zonal capillary network rearrangements. CONCLUSIONS Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of ageing-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.",
journal = "Annals of Anatomy",
title = "The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.",
volume = "230",
doi = "10.1016/j.aanat.2020.151487",
pages = "151487"
}

Ajdžanović, V., Miler, M., Živanović, J., Filipović, B., Šošić-Jurjević, B., Popovska-Perčinić, F.,& Milošević, V.. (2020). The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.. in Annals of Anatomy, 230, 151487.
https://doi.org/10.1016/j.aanat.2020.151487

Ajdžanović V, Miler M, Živanović J, Filipović B, Šošić-Jurjević B, Popovska-Perčinić F, Milošević V. The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.. in Annals of Anatomy. 2020;230:151487.
doi:10.1016/j.aanat.2020.151487 .

Ajdžanović, Vladimir, Miler, Marko, Živanović, Jasmina, Filipović, Branko, Šošić-Jurjević, Branka , Popovska-Perčinić, Florina, Milošević, Verica, "The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study." in Annals of Anatomy, 230 (2020):151487,
https://doi.org/10.1016/j.aanat.2020.151487 . .