TY  - JOUR
AU  - Mattoo, Autar
AU  - Cavigelli, Michel A.
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Maksimović, Vuk
AU  - Kramer, Matthew
AU  - Kaur, Bhavneet
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Roberts, Daniel
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6255
AB  - Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.
PB  - Frontiers
T2  - Frontiers in Sustainable Food Systems
T1  - Maize metabolomics in relation to cropping system and growing year
VL  - 7
DO  - 10.3389/fsufs.2023.1130089
SP  - 1130089
ER  - 

@article{
author = "Mattoo, Autar and Cavigelli, Michel A. and Mišić, Danijela and Gašić, Uroš and Maksimović, Vuk and Kramer, Matthew and Kaur, Bhavneet and Matekalo, Dragana and Nestorović Živković, Jasmina and Roberts, Daniel",
year = "2023",
abstract = "Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.",
publisher = "Frontiers",
journal = "Frontiers in Sustainable Food Systems",
title = "Maize metabolomics in relation to cropping system and growing year",
volume = "7",
doi = "10.3389/fsufs.2023.1130089",
pages = "1130089"
}

Mattoo, A., Cavigelli, M. A., Mišić, D., Gašić, U., Maksimović, V., Kramer, M., Kaur, B., Matekalo, D., Nestorović Živković, J.,& Roberts, D.. (2023). Maize metabolomics in relation to cropping system and growing year. in Frontiers in Sustainable Food Systems
Frontiers., 7, 1130089.
https://doi.org/10.3389/fsufs.2023.1130089

Mattoo A, Cavigelli MA, Mišić D, Gašić U, Maksimović V, Kramer M, Kaur B, Matekalo D, Nestorović Živković J, Roberts D. Maize metabolomics in relation to cropping system and growing year. in Frontiers in Sustainable Food Systems. 2023;7:1130089.
doi:10.3389/fsufs.2023.1130089 .

Mattoo, Autar, Cavigelli, Michel A., Mišić, Danijela, Gašić, Uroš, Maksimović, Vuk, Kramer, Matthew, Kaur, Bhavneet, Matekalo, Dragana, Nestorović Živković, Jasmina, Roberts, Daniel, "Maize metabolomics in relation to cropping system and growing year" in Frontiers in Sustainable Food Systems, 7 (2023):1130089,
https://doi.org/10.3389/fsufs.2023.1130089 . .

TY  - CONF
AU  - Jovanović, Mirna
AU  - Milosavljević, Dragica
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Milivojević, Jasminka
AU  - Đorđević, Ana
AU  - Brkljačić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6238
AB  - Insulin resistance is a state where a normal amount of insulin can’t provoke an appropriate metabolic response. Insulin promotes membrane trafficking of the glucose transporter GLUT4 from the storage vesicles to the plasma membrane in white adipose tissue. Adipocytes use glucose for lipogenesis and store the energy as lipid droplets. If adipocytes are unable to uptake glucose, a chronic state of hyperglycemia is developed, with severe health consequences. Polyphenols are natural anti-inflammatory and antioxidant agents. Food rich in polyphenols has been suggested to exert an ameliorative effect on restoring insulin sensitivity, with the main identified target being AMPK1,2, one of the key sensors of intracellular energy. Here, we tested the effect of methanol extracts from three newly introduced strawberry (Fragaria x ananassa, Duch.) cultivars – 'Aprika', 'Sandra' and 'Quicky' on glucose metabolism in 3T3-F442A adipocytes. It was determined that 'Aprika' has the highest total phenolic content, relative to the other two cultivars. After 72-h exposure, none of the strawberry cultivars affected adipocyte cell growth significantly. Protein expression analysis of the differentiated adipocytes suggested 'Aprika', but not the other two cultivars, significantly increased the AMPK expression, as well as GLUT4, thus increasing glucose uptake. Strawberry extracts did not significantly affect the differentiation of adipocytes (SIRT1 and PPARγ), nor the fatty acid synthesis (ACC). Conclusively, the 'Aprika' methanol extract with high phenolic content exerts an ameliorative effect on glucose uptake, presumably through activation of the AMPK-dependent mechanism of GLUT4 trafficking. The systemic effects of the ‘Aprika’ cultivar should be further investigated. Implications of the research are decreased hyperglycemia in obese and diabetic patients, by the introduction of the 'Aprika' strawberry cultivar into everyday diet. References 1. Zhao L, Zou T, Gomez NA et al. Raspberry alleviates obesity-induced inflammation and insulin resistance in skeletal muscle through activation of AMP-activated protein kinase (AMPK) α1. Nutr & Diabetes, 2018, 8, 39. 2. Xu W, Luo Y, Yin J, Luo F. Targeting AMPK signaling by polyphenols: A novel strategy for tackling aging. Food & Function. 2023.
PB  - Belgrade: Faculty of Chemistry
C3  - Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
T1  - Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes
SP  - 131
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6238
ER  - 

@conference{
author = "Jovanović, Mirna and Milosavljević, Dragica and Dragišić Maksimović, Jelena and Maksimović, Vuk and Milivojević, Jasminka and Đorđević, Ana and Brkljačić, Jelena",
year = "2023",
abstract = "Insulin resistance is a state where a normal amount of insulin can’t provoke an appropriate metabolic response. Insulin promotes membrane trafficking of the glucose transporter GLUT4 from the storage vesicles to the plasma membrane in white adipose tissue. Adipocytes use glucose for lipogenesis and store the energy as lipid droplets. If adipocytes are unable to uptake glucose, a chronic state of hyperglycemia is developed, with severe health consequences. Polyphenols are natural anti-inflammatory and antioxidant agents. Food rich in polyphenols has been suggested to exert an ameliorative effect on restoring insulin sensitivity, with the main identified target being AMPK1,2, one of the key sensors of intracellular energy. Here, we tested the effect of methanol extracts from three newly introduced strawberry (Fragaria x ananassa, Duch.) cultivars – 'Aprika', 'Sandra' and 'Quicky' on glucose metabolism in 3T3-F442A adipocytes. It was determined that 'Aprika' has the highest total phenolic content, relative to the other two cultivars. After 72-h exposure, none of the strawberry cultivars affected adipocyte cell growth significantly. Protein expression analysis of the differentiated adipocytes suggested 'Aprika', but not the other two cultivars, significantly increased the AMPK expression, as well as GLUT4, thus increasing glucose uptake. Strawberry extracts did not significantly affect the differentiation of adipocytes (SIRT1 and PPARγ), nor the fatty acid synthesis (ACC). Conclusively, the 'Aprika' methanol extract with high phenolic content exerts an ameliorative effect on glucose uptake, presumably through activation of the AMPK-dependent mechanism of GLUT4 trafficking. The systemic effects of the ‘Aprika’ cultivar should be further investigated. Implications of the research are decreased hyperglycemia in obese and diabetic patients, by the introduction of the 'Aprika' strawberry cultivar into everyday diet. References 1. Zhao L, Zou T, Gomez NA et al. Raspberry alleviates obesity-induced inflammation and insulin resistance in skeletal muscle through activation of AMP-activated protein kinase (AMPK) α1. Nutr & Diabetes, 2018, 8, 39. 2. Xu W, Luo Y, Yin J, Luo F. Targeting AMPK signaling by polyphenols: A novel strategy for tackling aging. Food & Function. 2023.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
title = "Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes",
pages = "131-132",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6238"
}

Jovanović, M., Milosavljević, D., Dragišić Maksimović, J., Maksimović, V., Milivojević, J., Đorđević, A.,& Brkljačić, J.. (2023). Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
Belgrade: Faculty of Chemistry., 131-132.
https://hdl.handle.net/21.15107/rcub_ibiss_6238

Jovanović M, Milosavljević D, Dragišić Maksimović J, Maksimović V, Milivojević J, Đorđević A, Brkljačić J. Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. 2023;:131-132.
https://hdl.handle.net/21.15107/rcub_ibiss_6238 .

Jovanović, Mirna, Milosavljević, Dragica, Dragišić Maksimović, Jelena, Maksimović, Vuk, Milivojević, Jasminka, Đorđević, Ana, Brkljačić, Jelena, "Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes" in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia (2023):131-132,
https://hdl.handle.net/21.15107/rcub_ibiss_6238 .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Živanović, Branka
AU  - Maksimović, Vuk
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5063
AB  - Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5063
ER  - 

@conference{
author = "Stanišić, Mariana and Smailagić, Dijana and Živanović, Branka and Maksimović, Vuk and Ninković, Slavica and Banjac, Nevena",
year = "2022",
abstract = "Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings",
pages = "44",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5063"
}

Stanišić, M., Smailagić, D., Živanović, B., Maksimović, V., Ninković, S.,& Banjac, N.. (2022). What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063

Stanišić M, Smailagić D, Živanović B, Maksimović V, Ninković S, Banjac N. What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

Stanišić, Mariana, Smailagić, Dijana, Živanović, Branka, Maksimović, Vuk, Ninković, Slavica, Banjac, Nevena, "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):44,
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragićević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Perišić, Mirjana
AU  - Mišić, Danijela
PY  - 2021
UR  - https://doi.org/10.3390/plants10010142
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4232
AB  - Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.
PB  - MDPI AG
T2  - Plants
T1  - Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro
IS  - 1
VL  - 10
DO  - 10.3390/plants10010142
SP  - 1
EP  - 20
ER  - 

@article{
author = "Dmitrović, Slavica and Dragićević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Perišić, Mirjana and Mišić, Danijela",
year = "2021",
abstract = "Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.",
publisher = "MDPI AG",
journal = "Plants",
title = "Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro",
number = "1",
volume = "10",
doi = "10.3390/plants10010142",
pages = "1-20"
}

Dmitrović, S., Dragićević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D., Perišić, M.,& Mišić, D.. (2021). Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro. in Plants
MDPI AG., 10(1), 1-20.
https://doi.org/10.3390/plants10010142

Dmitrović S, Dragićević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Perišić M, Mišić D. Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro. in Plants. 2021;10(1):1-20.
doi:10.3390/plants10010142 .

Dmitrović, Slavica, Dragićević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Perišić, Mirjana, Mišić, Danijela, "Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro" in Plants, 10, no. 1 (2021):1-20,
https://doi.org/10.3390/plants10010142 . .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Stanković-Jeremić, Jovana
AU  - Maksimović, Vuk
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - internal-pdf://Stupar et al. - 2021 - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf St.pdf
UR  - https://www.mdpi.com/2223-7747/10/1/172
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4147
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI AG
T2  - Plants
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
SP  - 172
ER  - 

@article{
author = "Stupar, Sofija and Dragićević, Milan and Tešević, Vele and Stanković-Jeremić, Jovana and Maksimović, Vuk and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI AG",
journal = "Plants",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172",
pages = "172"
}

Stupar, S., Dragićević, M., Tešević, V., Stanković-Jeremić, J., Maksimović, V., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants
MDPI AG., 10(1), 172.
https://doi.org/10.3390/plants10010172

Stupar S, Dragićević M, Tešević V, Stanković-Jeremić J, Maksimović V, Ćosić T, Devrnja N, Tubić L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants. 2021;10(1):172.
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragićević, Milan, Tešević, Vele, Stanković-Jeremić, Jovana, Maksimović, Vuk, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants, 10, no. 1 (2021):172,
https://doi.org/10.3390/plants10010172 . .

TY  - JOUR
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Maksimović, Vuk
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Božunović, Jelena
AU  - Banjanac, Tijana
AU  - Mišić, Danijela
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3699
AB  - Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH• and ABTS•+), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(–)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.
PB  - Elsevier B.V.
T2  - South African Journal of Botany
T1  - Antioxidant and antimicrobial activity of two Asplenium species
VL  - 132
DO  - 10.1016/j.sajb.2020.03.034
SP  - 180
EP  - 187
ER  - 

@article{
author = "Živković, Suzana and Milutinović, Milica and Maksimović, Vuk and Ćirić, Ana and Ivanov, Marija and Božunović, Jelena and Banjanac, Tijana and Mišić, Danijela",
year = "2020",
abstract = "Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH• and ABTS•+), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(–)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.",
publisher = "Elsevier B.V.",
journal = "South African Journal of Botany",
title = "Antioxidant and antimicrobial activity of two Asplenium species",
volume = "132",
doi = "10.1016/j.sajb.2020.03.034",
pages = "180-187"
}

Živković, S., Milutinović, M., Maksimović, V., Ćirić, A., Ivanov, M., Božunović, J., Banjanac, T.,& Mišić, D.. (2020). Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany
Elsevier B.V.., 132, 180-187.
https://doi.org/10.1016/j.sajb.2020.03.034

Živković S, Milutinović M, Maksimović V, Ćirić A, Ivanov M, Božunović J, Banjanac T, Mišić D. Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany. 2020;132:180-187.
doi:10.1016/j.sajb.2020.03.034 .

Živković, Suzana, Milutinović, Milica, Maksimović, Vuk, Ćirić, Ana, Ivanov, Marija, Božunović, Jelena, Banjanac, Tijana, Mišić, Danijela, "Antioxidant and antimicrobial activity of two Asplenium species" in South African Journal of Botany, 132 (2020):180-187,
https://doi.org/10.1016/j.sajb.2020.03.034 . .

TY  - JOUR
AU  - Cvetić Antić, Tijana
AU  - Janošević, Dušica
AU  - Maksimović, Vuk M.
AU  - Živić, Miroslav
AU  - Budimir, Snežana
AU  - Glamočlija, Jasmina
AU  - Mitrović, Aleksandra Lj
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3730
AB  - We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
PB  - NLM (Medline)
T2  - Journal of Plant Physiology
T1  - Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
VL  - 244
DO  - 10.1016/j.jplph.2019.153086
SP  - 153086
ER  - 

@article{
author = "Cvetić Antić, Tijana and Janošević, Dušica and Maksimović, Vuk M. and Živić, Miroslav and Budimir, Snežana and Glamočlija, Jasmina and Mitrović, Aleksandra Lj",
year = "2020",
abstract = "We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.",
publisher = "NLM (Medline)",
journal = "Journal of Plant Physiology",
title = "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro",
volume = "244",
doi = "10.1016/j.jplph.2019.153086",
pages = "153086"
}

Cvetić Antić, T., Janošević, D., Maksimović, V. M., Živić, M., Budimir, S., Glamočlija, J.,& Mitrović, A. L.. (2020). Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology
NLM (Medline)., 244, 153086.
https://doi.org/10.1016/j.jplph.2019.153086

Cvetić Antić T, Janošević D, Maksimović VM, Živić M, Budimir S, Glamočlija J, Mitrović AL. Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology. 2020;244:153086.
doi:10.1016/j.jplph.2019.153086 .

Cvetić Antić, Tijana, Janošević, Dušica, Maksimović, Vuk M., Živić, Miroslav, Budimir, Snežana, Glamočlija, Jasmina, Mitrović, Aleksandra Lj, "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro" in Journal of Plant Physiology, 244 (2020):153086,
https://doi.org/10.1016/j.jplph.2019.153086 . .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragićević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Mišić, Danijela
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161719300525?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3342
AB  - Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.
T2  - Journal of Plant Physiology
T1  - Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.
VL  - 237
DO  - 10.1016/j.jplph.2019.04.006
SP  - 87
EP  - 94
ER  - 

@article{
author = "Dmitrović, Slavica and Dragićević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Mišić, Danijela",
year = "2019",
abstract = "Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.",
journal = "Journal of Plant Physiology",
title = "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.",
volume = "237",
doi = "10.1016/j.jplph.2019.04.006",
pages = "87-94"
}

Dmitrović, S., Dragićević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D.,& Mišić, D.. (2019). Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology, 237, 87-94.
https://doi.org/10.1016/j.jplph.2019.04.006

Dmitrović S, Dragićević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Mišić D. Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology. 2019;237:87-94.
doi:10.1016/j.jplph.2019.04.006 .

Dmitrović, Slavica, Dragićević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Mišić, Danijela, "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh." in Journal of Plant Physiology, 237 (2019):87-94,
https://doi.org/10.1016/j.jplph.2019.04.006 . .

TY  - JOUR
AU  - Milutinović, Milena G.
AU  - Maksimović, Vuk M.
AU  - Cvetković, Danijela M.
AU  - Nikodijević, Danijela D.
AU  - Stanković, Milan S.
AU  - Pešić, Milica
AU  - Marković, Snežana D.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0378874119303010?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3406
AB  - ETHNOPHARMACOLOGICAL RELEVANCE Teucrum chamaedrys L. is one of the known medicinal plants, useful for treatment of various health problems, especially digestive. In this study, we investigated methanol, ethyl-acetate and acetone extracts of T. chamaedrys in respect to their anticancer properties in SW480 colorectal cancer cells. MATERIALS AND METHODS Cytotoxicity and proapoptotic potential were assessed by MTT cell viability assay and AO/EB double staining. Molecular mechanisms of induced apoptosis were determined by monitoring Fas receptor protein expression through immunofluorescence, Caspase 8 and 9 activity, as well as concentrations of O2.- spectrophotometrically. Additionally, mRNA expression of biotransformation enzymes (CYP1A1, CYP1B1, GSTP1) and membrane transporters (MRP1 and MRP2) involved in drug resistance were investigated by qPCR method. Qualitative analysis of individual phenolic compounds was performed by reversed phase HPLC-MS analysis. RESULTS Methanol extract shows the best cytotoxicity and selectivity compared to ethyl-acetate and acetone extracts, mainly causing apoptosis of SW480 cells, without affecting normal HaCaT keratinocytes. The increased expression of Fas receptor protein and caspase 8 activity indicate that the death receptor-mediated pathway plays a crucial role in the observed apoptosis. The increased caspase 9 activity and O2.- concentration suggest that mitochondria are also involved in the apoptosis. T. chamaedrys methanol extract inhibits mRNA expression of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 in SW480 cells. CONCLUSIONS Induction of apoptosis and inhibition of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 mRNA expression implies that T. chamaedrys can serve as a valuable source of bioactive compounds as dietary supplements or selective anticancer agents, with the ability to induce apoptosis and modulate drug resistance in colorectal cancer cells.
T2  - Journal of Ethnopharmacology
T1  - Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.
VL  - 240
DO  - 10.1016/j.jep.2019.111951
SP  - 111951
ER  - 

@article{
author = "Milutinović, Milena G. and Maksimović, Vuk M. and Cvetković, Danijela M. and Nikodijević, Danijela D. and Stanković, Milan S. and Pešić, Milica and Marković, Snežana D.",
year = "2019",
abstract = "ETHNOPHARMACOLOGICAL RELEVANCE Teucrum chamaedrys L. is one of the known medicinal plants, useful for treatment of various health problems, especially digestive. In this study, we investigated methanol, ethyl-acetate and acetone extracts of T. chamaedrys in respect to their anticancer properties in SW480 colorectal cancer cells. MATERIALS AND METHODS Cytotoxicity and proapoptotic potential were assessed by MTT cell viability assay and AO/EB double staining. Molecular mechanisms of induced apoptosis were determined by monitoring Fas receptor protein expression through immunofluorescence, Caspase 8 and 9 activity, as well as concentrations of O2.- spectrophotometrically. Additionally, mRNA expression of biotransformation enzymes (CYP1A1, CYP1B1, GSTP1) and membrane transporters (MRP1 and MRP2) involved in drug resistance were investigated by qPCR method. Qualitative analysis of individual phenolic compounds was performed by reversed phase HPLC-MS analysis. RESULTS Methanol extract shows the best cytotoxicity and selectivity compared to ethyl-acetate and acetone extracts, mainly causing apoptosis of SW480 cells, without affecting normal HaCaT keratinocytes. The increased expression of Fas receptor protein and caspase 8 activity indicate that the death receptor-mediated pathway plays a crucial role in the observed apoptosis. The increased caspase 9 activity and O2.- concentration suggest that mitochondria are also involved in the apoptosis. T. chamaedrys methanol extract inhibits mRNA expression of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 in SW480 cells. CONCLUSIONS Induction of apoptosis and inhibition of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 mRNA expression implies that T. chamaedrys can serve as a valuable source of bioactive compounds as dietary supplements or selective anticancer agents, with the ability to induce apoptosis and modulate drug resistance in colorectal cancer cells.",
journal = "Journal of Ethnopharmacology",
title = "Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.",
volume = "240",
doi = "10.1016/j.jep.2019.111951",
pages = "111951"
}

Milutinović, M. G., Maksimović, V. M., Cvetković, D. M., Nikodijević, D. D., Stanković, M. S., Pešić, M.,& Marković, S. D.. (2019). Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.. in Journal of Ethnopharmacology, 240, 111951.
https://doi.org/10.1016/j.jep.2019.111951

Milutinović MG, Maksimović VM, Cvetković DM, Nikodijević DD, Stanković MS, Pešić M, Marković SD. Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.. in Journal of Ethnopharmacology. 2019;240:111951.
doi:10.1016/j.jep.2019.111951 .

Milutinović, Milena G., Maksimović, Vuk M., Cvetković, Danijela M., Nikodijević, Danijela D., Stanković, Milan S., Pešić, Milica, Marković, Snežana D., "Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells." in Journal of Ethnopharmacology, 240 (2019):111951,
https://doi.org/10.1016/j.jep.2019.111951 . .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Spasojević, Dragica
AU  - Orlovska, Irina
AU  - Kozyrovska, Natalia
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Dmitrović, Svetlana
AU  - Matović, Branko
AU  - Tasić, Nikola
AU  - Maksimović, Vuk
AU  - Sosnin, Mikhail
AU  - Radotić, Ksenija
PY  - 2018
UR  - https://doi.org/10.1016/j.ijbiomac.2018.06.067
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3102
AB  - Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.
T2  - International Journal of Biological Macromolecules
T1  - Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing
DO  - 10.1016/j.ijbiomac.2018.06.067
ER  - 

@article{
author = "Zmejkoski, Danica and Spasojević, Dragica and Orlovska, Irina and Kozyrovska, Natalia and Soković, Marina and Glamočlija, Jasmina and Dmitrović, Svetlana and Matović, Branko and Tasić, Nikola and Maksimović, Vuk and Sosnin, Mikhail and Radotić, Ksenija",
year = "2018",
abstract = "Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.",
journal = "International Journal of Biological Macromolecules",
title = "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing",
doi = "10.1016/j.ijbiomac.2018.06.067"
}

Zmejkoski, D., Spasojević, D., Orlovska, I., Kozyrovska, N., Soković, M., Glamočlija, J., Dmitrović, S., Matović, B., Tasić, N., Maksimović, V., Sosnin, M.,& Radotić, K.. (2018). Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules.
https://doi.org/10.1016/j.ijbiomac.2018.06.067

Zmejkoski D, Spasojević D, Orlovska I, Kozyrovska N, Soković M, Glamočlija J, Dmitrović S, Matović B, Tasić N, Maksimović V, Sosnin M, Radotić K. Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules. 2018;.
doi:10.1016/j.ijbiomac.2018.06.067 .

Zmejkoski, Danica, Spasojević, Dragica, Orlovska, Irina, Kozyrovska, Natalia, Soković, Marina, Glamočlija, Jasmina, Dmitrović, Svetlana, Matović, Branko, Tasić, Nikola, Maksimović, Vuk, Sosnin, Mikhail, Radotić, Ksenija, "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing" in International Journal of Biological Macromolecules (2018),
https://doi.org/10.1016/j.ijbiomac.2018.06.067 . .

TY  - CONF
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Šiler, Branislav
AU  - Božić, Dragana
AU  - Mišić, Danijela
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4399
AB  - Historically, ferns have been an important source of drugs, food, and fibers. Nevertheless, very few works have been done on their phytochemical characterization. Moreover, the haploid gametophyte phase of ferns has, probably due to the fragile structure, microscopic dimensions, and difficult collection, only rarely been the subject of phytochemical investigations. With the aim to support the ethnopharmacological importance of Asplenium species, the present work focuses on evaluating the phenolic content and antioxidant properties of sporophytes and gametophytes of Asplenium adiantum-nigrum L. and A. ruta-muraria L, two fern species widely distributed in Europe. Methanol extracts of
gametophytes and sporophytes (fronds, rhizomes and roots) were subjected to UHPLC/DAD/(−)HESI-MS/MS analysis, and further to the evaluation of their scavenging capacities against ABTS•+ and DPPH•.
Phenolics content and antioxidant activity of analyzed Asplenium species showed species-specific and life cyclespecific patterns. Identified phenolic compounds belong to the classes of phenolic acids (hydroxybenzoic and hydroxycynnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones. Sporophytes of A. adiantum-nigrum are especially rich in xanthones (mangiferin and mangiferin glycoside), potent bioactive compounds. Besides xanthones, gametophytes contain significant amounts of flavan-3-ols (epigallocatechin and epigallocatechin gallate). Flavan-3-ols are also recognized as the major phenolic compounds in both A. ruta-muraria sporophytes (gallocatechin gallate and epigallocatechin gallate) and gametophytes (gallocatechin and catechin). The excellent antioxidant activity of A. adiantum-nigrum rhizomes and A. ruta-muraria fronds, against both DPPH and ABTS radicals, could be ascribed to the high content of xanthones and flavan-3-ols, respectively. The results of the present study candidate these two fern species as potent antioxidant agents, thus supporting their traditional use.
PB  - Belgrade: University of Belgrade
C3  - Programme and Book of Abstracts of the UNIFOOD Conference
T1  - Phenolics content and antioxidant activity of two Asplenium species: life cycle-specific patterns
SP  - BKH10/FQS10 (U/O)
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4399
ER  - 

@conference{
author = "Živković, Suzana and Maksimović, Vuk and Milutinović, Milica and Božunović, Jelena and Aničić, Neda and Šiler, Branislav and Božić, Dragana and Mišić, Danijela",
year = "2018",
abstract = "Historically, ferns have been an important source of drugs, food, and fibers. Nevertheless, very few works have been done on their phytochemical characterization. Moreover, the haploid gametophyte phase of ferns has, probably due to the fragile structure, microscopic dimensions, and difficult collection, only rarely been the subject of phytochemical investigations. With the aim to support the ethnopharmacological importance of Asplenium species, the present work focuses on evaluating the phenolic content and antioxidant properties of sporophytes and gametophytes of Asplenium adiantum-nigrum L. and A. ruta-muraria L, two fern species widely distributed in Europe. Methanol extracts of
gametophytes and sporophytes (fronds, rhizomes and roots) were subjected to UHPLC/DAD/(−)HESI-MS/MS analysis, and further to the evaluation of their scavenging capacities against ABTS•+ and DPPH•.
Phenolics content and antioxidant activity of analyzed Asplenium species showed species-specific and life cyclespecific patterns. Identified phenolic compounds belong to the classes of phenolic acids (hydroxybenzoic and hydroxycynnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones. Sporophytes of A. adiantum-nigrum are especially rich in xanthones (mangiferin and mangiferin glycoside), potent bioactive compounds. Besides xanthones, gametophytes contain significant amounts of flavan-3-ols (epigallocatechin and epigallocatechin gallate). Flavan-3-ols are also recognized as the major phenolic compounds in both A. ruta-muraria sporophytes (gallocatechin gallate and epigallocatechin gallate) and gametophytes (gallocatechin and catechin). The excellent antioxidant activity of A. adiantum-nigrum rhizomes and A. ruta-muraria fronds, against both DPPH and ABTS radicals, could be ascribed to the high content of xanthones and flavan-3-ols, respectively. The results of the present study candidate these two fern species as potent antioxidant agents, thus supporting their traditional use.",
publisher = "Belgrade: University of Belgrade",
journal = "Programme and Book of Abstracts of the UNIFOOD Conference",
title = "Phenolics content and antioxidant activity of two Asplenium species: life cycle-specific patterns",
pages = "BKH10/FQS10 (U/O)",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4399"
}

Živković, S., Maksimović, V., Milutinović, M., Božunović, J., Aničić, N., Šiler, B., Božić, D.,& Mišić, D.. (2018). Phenolics content and antioxidant activity of two Asplenium species: life cycle-specific patterns. in Programme and Book of Abstracts of the UNIFOOD Conference
Belgrade: University of Belgrade., BKH10/FQS10 (U/O).
https://hdl.handle.net/21.15107/rcub_ibiss_4399

Živković S, Maksimović V, Milutinović M, Božunović J, Aničić N, Šiler B, Božić D, Mišić D. Phenolics content and antioxidant activity of two Asplenium species: life cycle-specific patterns. in Programme and Book of Abstracts of the UNIFOOD Conference. 2018;:BKH10/FQS10 (U/O).
https://hdl.handle.net/21.15107/rcub_ibiss_4399 .

Živković, Suzana, Maksimović, Vuk, Milutinović, Milica, Božunović, Jelena, Aničić, Neda, Šiler, Branislav, Božić, Dragana, Mišić, Danijela, "Phenolics content and antioxidant activity of two Asplenium species: life cycle-specific patterns" in Programme and Book of Abstracts of the UNIFOOD Conference (2018):BKH10/FQS10 (U/O),
https://hdl.handle.net/21.15107/rcub_ibiss_4399 .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Spasojević, Dragica
AU  - Orlovska, Irina
AU  - Kozyrovska, Natalia
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Dmitrović, Svetlana
AU  - Matović, Branko
AU  - Tasić, Nikola
AU  - Maksimović, Vuk
AU  - Sosnin, Mikhail
AU  - Radotić, Ksenija
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0141813018320919?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3101
AB  - Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.
T2  - International Journal of Biological Macromolecules
T1  - Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing.
IS  - Pt A
VL  - 118
DO  - 10.1016/j.ijbiomac.2018.06.067
SP  - 494
EP  - 503
ER  - 

@article{
author = "Zmejkoski, Danica and Spasojević, Dragica and Orlovska, Irina and Kozyrovska, Natalia and Soković, Marina and Glamočlija, Jasmina and Dmitrović, Svetlana and Matović, Branko and Tasić, Nikola and Maksimović, Vuk and Sosnin, Mikhail and Radotić, Ksenija",
year = "2018",
abstract = "Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.",
journal = "International Journal of Biological Macromolecules",
title = "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing.",
number = "Pt A",
volume = "118",
doi = "10.1016/j.ijbiomac.2018.06.067",
pages = "494-503"
}

Zmejkoski, D., Spasojević, D., Orlovska, I., Kozyrovska, N., Soković, M., Glamočlija, J., Dmitrović, S., Matović, B., Tasić, N., Maksimović, V., Sosnin, M.,& Radotić, K.. (2018). Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing.. in International Journal of Biological Macromolecules, 118(Pt A), 494-503.
https://doi.org/10.1016/j.ijbiomac.2018.06.067

Zmejkoski D, Spasojević D, Orlovska I, Kozyrovska N, Soković M, Glamočlija J, Dmitrović S, Matović B, Tasić N, Maksimović V, Sosnin M, Radotić K. Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing.. in International Journal of Biological Macromolecules. 2018;118(Pt A):494-503.
doi:10.1016/j.ijbiomac.2018.06.067 .

Zmejkoski, Danica, Spasojević, Dragica, Orlovska, Irina, Kozyrovska, Natalia, Soković, Marina, Glamočlija, Jasmina, Dmitrović, Svetlana, Matović, Branko, Tasić, Nikola, Maksimović, Vuk, Sosnin, Mikhail, Radotić, Ksenija, "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing." in International Journal of Biological Macromolecules, 118, no. Pt A (2018):494-503,
https://doi.org/10.1016/j.ijbiomac.2018.06.067 . .

TY  - CONF
AU  - Dmitrović, Slavica
AU  - Dragićević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Perišić, Mirjana
AU  - Mišić, Danijela
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4293
AB  - Phosphinothricin, active ingredient of the commercial herbicide BASTA, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation, which leads to the elevation of ammonium levels in plants and further to various physiological alternations, ammonium toxicity and lethality. Nepeta rtanjensis Diklić & Milojević essential oil (NrEO), rich in iridoid monoterpenoids nepetalactones, has been previously highlighted as a potential bioherbicide inducing oxidative stress in model plants. Interestingly, simultaneous foliar application of NrEO and BASTA, two agents showing differential mode of herbicidal action, suspends BASTA-induced toxicity in Arabidopsis thaliana plants by maintaining sub-toxic and/or sub-lethal ammonium concentration in tissues. NrEO effectively reduces BASTA efficacy, and the degree of antagonism between these agents escalates at increasing BASTA and NrEO levels. Regulation of GS activity, as influenced by BASTA, NrEO, and their joint action, partially occurs at transcriptional, posttranscriptional, and/or posttranslational levels, and is organ-specific. Furthermore, BASTA and NrEO interaction mitigates the effects of these agents, applied independently, on chlorophyll, soluble sugars and organic acids metabolism. Results suggest the existence of complex regulatory mechanisms determining antagonistic BASTA and NrEO interaction, and highlight the possible applications of the BASTA/NrEO mixture in agricultural practice. Furthermore, the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils, is suggested here as a environment-friendly approach for weed control.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4293
ER  - 

@conference{
author = "Dmitrović, Slavica and Dragićević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Perišić, Mirjana and Mišić, Danijela",
year = "2018",
abstract = "Phosphinothricin, active ingredient of the commercial herbicide BASTA, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation, which leads to the elevation of ammonium levels in plants and further to various physiological alternations, ammonium toxicity and lethality. Nepeta rtanjensis Diklić & Milojević essential oil (NrEO), rich in iridoid monoterpenoids nepetalactones, has been previously highlighted as a potential bioherbicide inducing oxidative stress in model plants. Interestingly, simultaneous foliar application of NrEO and BASTA, two agents showing differential mode of herbicidal action, suspends BASTA-induced toxicity in Arabidopsis thaliana plants by maintaining sub-toxic and/or sub-lethal ammonium concentration in tissues. NrEO effectively reduces BASTA efficacy, and the degree of antagonism between these agents escalates at increasing BASTA and NrEO levels. Regulation of GS activity, as influenced by BASTA, NrEO, and their joint action, partially occurs at transcriptional, posttranscriptional, and/or posttranslational levels, and is organ-specific. Furthermore, BASTA and NrEO interaction mitigates the effects of these agents, applied independently, on chlorophyll, soluble sugars and organic acids metabolism. Results suggest the existence of complex regulatory mechanisms determining antagonistic BASTA and NrEO interaction, and highlight the possible applications of the BASTA/NrEO mixture in agricultural practice. Furthermore, the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils, is suggested here as a environment-friendly approach for weed control.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity",
pages = "49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4293"
}

Dmitrović, S., Dragićević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D., Perišić, M.,& Mišić, D.. (2018). Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 49.
https://hdl.handle.net/21.15107/rcub_ibiss_4293

Dmitrović S, Dragićević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Perišić M, Mišić D. Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:49.
https://hdl.handle.net/21.15107/rcub_ibiss_4293 .

Dmitrović, Slavica, Dragićević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Perišić, Mirjana, Mišić, Danijela, "Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):49,
https://hdl.handle.net/21.15107/rcub_ibiss_4293 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Dragičević, Milan B.
AU  - Šiler, Branislav
AU  - Nestorović Živković, Jasmina
AU  - Maksimović, Vuk M
AU  - Momčilović, Ivana
AU  - Nikolić, Miroslav
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1110
AB  - A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.
T2  - Journal of Plant Physiology
T1  - Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress
IS  - 13
VL  - 169
SP  - 93
EP  - 1289
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1110
ER  - 

@article{
author = "Mišić, Danijela and Dragičević, Milan B. and Šiler, Branislav and Nestorović Živković, Jasmina and Maksimović, Vuk M and Momčilović, Ivana and Nikolić, Miroslav",
year = "2012",
abstract = "A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.",
journal = "Journal of Plant Physiology",
title = "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress",
number = "13",
volume = "169",
pages = "93-1289",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1110"
}

Mišić, D., Dragičević, M. B., Šiler, B., Nestorović Živković, J., Maksimović, V. M., Momčilović, I.,& Nikolić, M.. (2012). Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology, 169(13), 93-1289.
https://hdl.handle.net/21.15107/rcub_ibiss_1110

Mišić D, Dragičević MB, Šiler B, Nestorović Živković J, Maksimović VM, Momčilović I, Nikolić M. Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology. 2012;169(13):93-1289.
https://hdl.handle.net/21.15107/rcub_ibiss_1110 .

Mišić, Danijela, Dragičević, Milan B., Šiler, Branislav, Nestorović Živković, Jasmina, Maksimović, Vuk M, Momčilović, Ivana, Nikolić, Miroslav, "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress" in Journal of Plant Physiology, 169, no. 13 (2012):93-1289,
https://hdl.handle.net/21.15107/rcub_ibiss_1110 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Nestorović Živković, Jasmina
AU  - Simonović, Ana
AU  - Maksimović, Vuk M
AU  - Budimir, Snežana
AU  - Janošević, Dušica A.
AU  - Đuricković, Milutin S
AU  - Nikolić, Miroslav
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1204
AB  - The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.
T2  - Plant Cell Tissue and Organ Culture
T1  - Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress
IS  - 3
VL  - 108
EP  - 400
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1204
ER  - 

@article{
author = "Mišić, Danijela and Šiler, Branislav and Nestorović Živković, Jasmina and Simonović, Ana and Maksimović, Vuk M and Budimir, Snežana and Janošević, Dušica A. and Đuricković, Milutin S and Nikolić, Miroslav",
year = "2012",
abstract = "The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress",
number = "3",
volume = "108",
pages = "400",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1204"
}

Mišić, D., Šiler, B., Nestorović Živković, J., Simonović, A., Maksimović, V. M., Budimir, S., Janošević, D. A., Đuricković, M. S.,& Nikolić, M.. (2012). Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture, 108(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1204

Mišić D, Šiler B, Nestorović Živković J, Simonović A, Maksimović VM, Budimir S, Janošević DA, Đuricković MS, Nikolić M. Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture. 2012;108(3):null-400.
https://hdl.handle.net/21.15107/rcub_ibiss_1204 .

Mišić, Danijela, Šiler, Branislav, Nestorović Živković, Jasmina, Simonović, Ana, Maksimović, Vuk M, Budimir, Snežana, Janošević, Dušica A., Đuricković, Milutin S, Nikolić, Miroslav, "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress" in Plant Cell Tissue and Organ Culture, 108, no. 3 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1204 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Dmitrović, Slavica
AU  - Đorđević, Mirka
AU  - Zdravković-Korać, Snežana
AU  - Nikolić, Radomirka R
AU  - Raspor, Martin
AU  - Ćosić, Tatjana
AU  - Maksimović, Vuk M
AU  - Živković, Suzana
AU  - Krstić Milošević, Dijana
AU  - Stanišić, Mariana
AU  - Ninković, Slavica
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1150
AB  - We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. (C) 2012 Elsevier GmbH. All rights reserved.
T2  - Journal of Plant Physiology
T1  - Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
IS  - 12
VL  - 169
DO  - 10.1016/j.jplph.2012.04.009
SP  - 323
EP  - 1211
ER  - 

@article{
author = "Banjac, Nevena and Dmitrović, Slavica and Đorđević, Mirka and Zdravković-Korać, Snežana and Nikolić, Radomirka R and Raspor, Martin and Ćosić, Tatjana and Maksimović, Vuk M and Živković, Suzana and Krstić Milošević, Dijana and Stanišić, Mariana and Ninković, Slavica",
year = "2012",
abstract = "We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. (C) 2012 Elsevier GmbH. All rights reserved.",
journal = "Journal of Plant Physiology",
title = "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays",
number = "12",
volume = "169",
doi = "10.1016/j.jplph.2012.04.009",
pages = "323-1211"
}

Banjac, N., Dmitrović, S., Đorđević, M., Zdravković-Korać, S., Nikolić, R. R., Raspor, M., Ćosić, T., Maksimović, V. M., Živković, S., Krstić Milošević, D., Stanišić, M.,& Ninković, S.. (2012). Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology, 169(12), 323-1211.
https://doi.org/10.1016/j.jplph.2012.04.009

Banjac N, Dmitrović S, Đorđević M, Zdravković-Korać S, Nikolić RR, Raspor M, Ćosić T, Maksimović VM, Živković S, Krstić Milošević D, Stanišić M, Ninković S. Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology. 2012;169(12):323-1211.
doi:10.1016/j.jplph.2012.04.009 .

Banjac, Nevena, Dmitrović, Slavica, Đorđević, Mirka, Zdravković-Korać, Snežana, Nikolić, Radomirka R, Raspor, Martin, Ćosić, Tatjana, Maksimović, Vuk M, Živković, Suzana, Krstić Milošević, Dijana, Stanišić, Mariana, Ninković, Slavica, "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays" in Journal of Plant Physiology, 169, no. 12 (2012):323-1211,
https://doi.org/10.1016/j.jplph.2012.04.009 . .

TY  - JOUR
AU  - Mašković, Pavle
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
AU  - Manojlović, Nedeljko T.
AU  - Radojković, Marija
AU  - Cvijović, Milica
AU  - Solujić, Slavica
PY  - 2012
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4675
AB  - The objective of this study was to evaluate the efficacy of the ethanolic extract of endemic plant Halacsya sendtneri in inhibiting the growing of the test fungi and bacteria as well as to determine its genotoxic potential and toxicity using the Allium anaphase-telophase assay. Minimum inhibitory concentrations (MIC) were determined for 15 indicator strains of pathogens, representing both bacteria and fungi. The highest susceptibility to the ethanolic extract of H. sendtneri was   exhibited by Pseudomonas glycinea (FSB4), (MIC=0.09 mg/ml) among the bacteria, and by Phialophora fastigiata (FSB81), (MIC=1.95 mg/ml) among the fungi. The composition of H. sendtneri extracts was also determined using HPLC analysis. Rosmarinic acid was found to be the dominant phenolic compound. The Allium anaphase-telophase genotoxicity assay revealed that the ethanolic extract of H. sendtneri at concentrations of 31.5 mg/l and below does not produce toxic or genotoxic effects. This is the first report of chemical constituents, genotoxic and antimicrobial activities of the endemic species, H.  sendtneri.
PB  - Warsaw: Versita
T2  - Central European Journal of Biology
T1  - Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler
IS  - 2
VL  - 7
DO  - 10.2478/s11535-012-0021-8
SP  - 327
EP  - 333
ER  - 

@article{
author = "Mašković, Pavle and Dragišić Maksimović, Jelena and Maksimović, Vuk and Blagojević, Jelena and Vujošević, Mladen and Manojlović, Nedeljko T. and Radojković, Marija and Cvijović, Milica and Solujić, Slavica",
year = "2012",
abstract = "The objective of this study was to evaluate the efficacy of the ethanolic extract of endemic plant Halacsya sendtneri in inhibiting the growing of the test fungi and bacteria as well as to determine its genotoxic potential and toxicity using the Allium anaphase-telophase assay. Minimum inhibitory concentrations (MIC) were determined for 15 indicator strains of pathogens, representing both bacteria and fungi. The highest susceptibility to the ethanolic extract of H. sendtneri was   exhibited by Pseudomonas glycinea (FSB4), (MIC=0.09 mg/ml) among the bacteria, and by Phialophora fastigiata (FSB81), (MIC=1.95 mg/ml) among the fungi. The composition of H. sendtneri extracts was also determined using HPLC analysis. Rosmarinic acid was found to be the dominant phenolic compound. The Allium anaphase-telophase genotoxicity assay revealed that the ethanolic extract of H. sendtneri at concentrations of 31.5 mg/l and below does not produce toxic or genotoxic effects. This is the first report of chemical constituents, genotoxic and antimicrobial activities of the endemic species, H.  sendtneri.",
publisher = "Warsaw: Versita",
journal = "Central European Journal of Biology",
title = "Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler",
number = "2",
volume = "7",
doi = "10.2478/s11535-012-0021-8",
pages = "327-333"
}

Mašković, P., Dragišić Maksimović, J., Maksimović, V., Blagojević, J., Vujošević, M., Manojlović, N. T., Radojković, M., Cvijović, M.,& Solujić, S.. (2012). Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler. in Central European Journal of Biology
Warsaw: Versita., 7(2), 327-333.
https://doi.org/10.2478/s11535-012-0021-8

Mašković P, Dragišić Maksimović J, Maksimović V, Blagojević J, Vujošević M, Manojlović NT, Radojković M, Cvijović M, Solujić S. Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler. in Central European Journal of Biology. 2012;7(2):327-333.
doi:10.2478/s11535-012-0021-8 .

Mašković, Pavle, Dragišić Maksimović, Jelena, Maksimović, Vuk, Blagojević, Jelena, Vujošević, Mladen, Manojlović, Nedeljko T., Radojković, Marija, Cvijović, Milica, Solujić, Slavica, "Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler" in Central European Journal of Biology, 7, no. 2 (2012):327-333,
https://doi.org/10.2478/s11535-012-0021-8 . .

TY  - JOUR
AU  - Stajić, Mirjana M
AU  - Glamočlija, Jasmina
AU  - Maksimović, Vuk M
AU  - Vukojević, Jelena B.
AU  - Simonić, Jasmina
AU  - Zervakis, George
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1328
AB  - Ganoderma lucidum is a well-known medicinal mushroom species in which polysaccharides are one of the major sources of biological activity. The species was considered as a species-complex due to significant variations in morphological, biochemical, and genetic features among populations with a worldwide distribution. This fact was the basis for setting the aim of this research: to study intraspecific diversity in polysaccharide production and intracellular sugar composition among selected G. lucidum strains. The presence of intraspecific diversity among 10 G. lucidum strains, from different areas worldwide, was noted. Values of produced mycelia biomass and intracellular polysaccharides were found in wide ranges (3.1-28.2 g L-1 and 20.0-53.3 mg g(-1), respectively), while differences in extracellular polysaccharide amounts were minor (0.2-1.5 mg mL(-1)). The significant quantitative and qualitative differences in intracellular sugar composition were noted. Glucose was the predominant sugar in almost all strains except one (HAI 447), where sucrose was dominant. The potential of polysaccharide production and intracellular sugar composition could be one more taxonomic criterion for strain characterization within G. lucidum. The differences in intracellular sugar composition and proportions could be reflected in features of produced polysaccharides and also in their biological activities.
T2  - International Journal of Medicinal Mushrooms
T1  - A Comparative Assessment of the Potential of Polysaccharide Production and Intracellular Sugar Composition within Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Aphyllophoromycetideae)
IS  - 2
VL  - 13
EP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1328
ER  - 

@article{
author = "Stajić, Mirjana M and Glamočlija, Jasmina and Maksimović, Vuk M and Vukojević, Jelena B. and Simonić, Jasmina and Zervakis, George",
year = "2011",
abstract = "Ganoderma lucidum is a well-known medicinal mushroom species in which polysaccharides are one of the major sources of biological activity. The species was considered as a species-complex due to significant variations in morphological, biochemical, and genetic features among populations with a worldwide distribution. This fact was the basis for setting the aim of this research: to study intraspecific diversity in polysaccharide production and intracellular sugar composition among selected G. lucidum strains. The presence of intraspecific diversity among 10 G. lucidum strains, from different areas worldwide, was noted. Values of produced mycelia biomass and intracellular polysaccharides were found in wide ranges (3.1-28.2 g L-1 and 20.0-53.3 mg g(-1), respectively), while differences in extracellular polysaccharide amounts were minor (0.2-1.5 mg mL(-1)). The significant quantitative and qualitative differences in intracellular sugar composition were noted. Glucose was the predominant sugar in almost all strains except one (HAI 447), where sucrose was dominant. The potential of polysaccharide production and intracellular sugar composition could be one more taxonomic criterion for strain characterization within G. lucidum. The differences in intracellular sugar composition and proportions could be reflected in features of produced polysaccharides and also in their biological activities.",
journal = "International Journal of Medicinal Mushrooms",
title = "A Comparative Assessment of the Potential of Polysaccharide Production and Intracellular Sugar Composition within Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Aphyllophoromycetideae)",
number = "2",
volume = "13",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1328"
}

Stajić, M. M., Glamočlija, J., Maksimović, V. M., Vukojević, J. B., Simonić, J.,& Zervakis, G.. (2011). A Comparative Assessment of the Potential of Polysaccharide Production and Intracellular Sugar Composition within Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Aphyllophoromycetideae). in International Journal of Medicinal Mushrooms, 13(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1328

Stajić MM, Glamočlija J, Maksimović VM, Vukojević JB, Simonić J, Zervakis G. A Comparative Assessment of the Potential of Polysaccharide Production and Intracellular Sugar Composition within Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Aphyllophoromycetideae). in International Journal of Medicinal Mushrooms. 2011;13(2):null-158.
https://hdl.handle.net/21.15107/rcub_ibiss_1328 .

Stajić, Mirjana M, Glamočlija, Jasmina, Maksimović, Vuk M, Vukojević, Jelena B., Simonić, Jasmina, Zervakis, George, "A Comparative Assessment of the Potential of Polysaccharide Production and Intracellular Sugar Composition within Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Aphyllophoromycetideae)" in International Journal of Medicinal Mushrooms, 13, no. 2 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1328 .

TY  - JOUR
AU  - Nestorović Živković, Jasmina
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Maksimović, Vuk M
AU  - Grubišić, Dragoljub V.
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1122
AB  - Rapid micropropagation of Nepeta rtanjensis, N. sibirica and N. nervosa was performed. Qualitative and quantitative nepetalactone content in methanol extracts of in vitro grown plants was analysed by reverse-phase HPLC coupled with UV and MS detection. Only trans,cis-nepetalactone was detected in shoots of N. rtanjensis, while cis,trans-nepetalactone stereoisomer was present in N. sibirica. No nepetalactone was observed in shoots of N. nervosa. The antimicrobial activity of methanol extracts, against eight bacterial and eight fungal species, was evaluated. All the tested extracts showed significant antibacterial and strong antifungal activity. However, N. rtanjensis extract exhibited the best antimicrobial potential. (C) 2010 Elsevier B.V. All rights reserved.
T2  - Fitoterapia
T1  - Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity
IS  - 6
VL  - 81
SP  - 1
EP  - 626
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1122
ER  - 

@article{
author = "Nestorović Živković, Jasmina and Mišić, Danijela and Šiler, Branislav and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Maksimović, Vuk M and Grubišić, Dragoljub V.",
year = "2010",
abstract = "Rapid micropropagation of Nepeta rtanjensis, N. sibirica and N. nervosa was performed. Qualitative and quantitative nepetalactone content in methanol extracts of in vitro grown plants was analysed by reverse-phase HPLC coupled with UV and MS detection. Only trans,cis-nepetalactone was detected in shoots of N. rtanjensis, while cis,trans-nepetalactone stereoisomer was present in N. sibirica. No nepetalactone was observed in shoots of N. nervosa. The antimicrobial activity of methanol extracts, against eight bacterial and eight fungal species, was evaluated. All the tested extracts showed significant antibacterial and strong antifungal activity. However, N. rtanjensis extract exhibited the best antimicrobial potential. (C) 2010 Elsevier B.V. All rights reserved.",
journal = "Fitoterapia",
title = "Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity",
number = "6",
volume = "81",
pages = "1-626",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1122"
}

Nestorović Živković, J., Mišić, D., Šiler, B., Soković, M., Glamočlija, J., Ćirić, A., Maksimović, V. M.,& Grubišić, D. V.. (2010). Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity. in Fitoterapia, 81(6), 1-626.
https://hdl.handle.net/21.15107/rcub_ibiss_1122

Nestorović Živković J, Mišić D, Šiler B, Soković M, Glamočlija J, Ćirić A, Maksimović VM, Grubišić DV. Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity. in Fitoterapia. 2010;81(6):1-626.
https://hdl.handle.net/21.15107/rcub_ibiss_1122 .

Nestorović Živković, Jasmina, Mišić, Danijela, Šiler, Branislav, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Maksimović, Vuk M, Grubišić, Dragoljub V., "Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity" in Fitoterapia, 81, no. 6 (2010):1-626,
https://hdl.handle.net/21.15107/rcub_ibiss_1122 .

TY  - JOUR
AU  - Harhaji-Trajković, Ljubica
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, Ivana D.
AU  - Momčilović, Miljana
AU  - Tufegdžić, Srđan J
AU  - Maksimović, Vuk M
AU  - Marjanović, Zaklina S
AU  - Stošić-Grujičić, Stanislava
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1487
AB  - Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.
T2  - Nutrition and Cancer-An International Journal
T1  - Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo
IS  - 5
VL  - 61
EP  - 707
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1487
ER  - 

@article{
author = "Harhaji-Trajković, Ljubica and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, Ivana D. and Momčilović, Miljana and Tufegdžić, Srđan J and Maksimović, Vuk M and Marjanović, Zaklina S and Stošić-Grujičić, Stanislava",
year = "2009",
abstract = "Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.",
journal = "Nutrition and Cancer-An International Journal",
title = "Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo",
number = "5",
volume = "61",
pages = "707",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1487"
}

Harhaji-Trajković, L., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I. D., Momčilović, M., Tufegdžić, S. J., Maksimović, V. M., Marjanović, Z. S.,& Stošić-Grujičić, S.. (2009). Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo. in Nutrition and Cancer-An International Journal, 61(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1487

Harhaji-Trajković L, Mijatović S, Maksimović-Ivanić D, Stojanović ID, Momčilović M, Tufegdžić SJ, Maksimović VM, Marjanović ZS, Stošić-Grujičić S. Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo. in Nutrition and Cancer-An International Journal. 2009;61(5):null-707.
https://hdl.handle.net/21.15107/rcub_ibiss_1487 .

Harhaji-Trajković, Ljubica, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, Ivana D., Momčilović, Miljana, Tufegdžić, Srđan J, Maksimović, Vuk M, Marjanović, Zaklina S, Stošić-Grujičić, Stanislava, "Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo" in Nutrition and Cancer-An International Journal, 61, no. 5 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1487 .

TY  - JOUR
AU  - Harhaji-Trajković, Ljubica
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, Ivana D.
AU  - Momčilović, Miljana
AU  - Maksimović, Vuk M.
AU  - Tufegdžić, Srđan J.
AU  - Marjanović, Zaklina S.
AU  - Mostarica-Stojković, Marija B.
AU  - Vučinić, Zeljko B.
AU  - Stošić-Grujičić, Stanislava
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1531
AB  - Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity. (c) 2008 Elsevier Ltd. All rights reserved.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study
IS  - 5
VL  - 46
DO  - 10.1016/j.fct.2008.01.027
SP  - 1825
EP  - 1833
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1531
ER  - 

@article{
author = "Harhaji-Trajković, Ljubica and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, Ivana D. and Momčilović, Miljana and Maksimović, Vuk M. and Tufegdžić, Srđan J. and Marjanović, Zaklina S. and Mostarica-Stojković, Marija B. and Vučinić, Zeljko B. and Stošić-Grujičić, Stanislava",
year = "2008",
abstract = "Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity. (c) 2008 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study",
number = "5",
volume = "46",
doi = "10.1016/j.fct.2008.01.027",
pages = "1825-1833",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1531"
}

Harhaji-Trajković, L., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I. D., Momčilović, M., Maksimović, V. M., Tufegdžić, S. J., Marjanović, Z. S., Mostarica-Stojković, M. B., Vučinić, Z. B.,& Stošić-Grujičić, S.. (2008). Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology
Elsevier., 46(5), 1825-1833.
https://doi.org/10.1016/j.fct.2008.01.027
https://hdl.handle.net/21.15107/rcub_ibiss_1531

Harhaji-Trajković L, Mijatović S, Maksimović-Ivanić D, Stojanović ID, Momčilović M, Maksimović VM, Tufegdžić SJ, Marjanović ZS, Mostarica-Stojković MB, Vučinić ZB, Stošić-Grujičić S. Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology. 2008;46(5):1825-1833.
doi:10.1016/j.fct.2008.01.027
https://hdl.handle.net/21.15107/rcub_ibiss_1531 .

Harhaji-Trajković, Ljubica, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, Ivana D., Momčilović, Miljana, Maksimović, Vuk M., Tufegdžić, Srđan J., Marjanović, Zaklina S., Mostarica-Stojković, Marija B., Vučinić, Zeljko B., Stošić-Grujičić, Stanislava, "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study" in Food and Chemical Toxicology, 46, no. 5 (2008):1825-1833,
https://doi.org/10.1016/j.fct.2008.01.027 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1531 .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Momčilović, Ivana
AU  - Krstić, Dijana
AU  - Maksimović, Vuk M
AU  - Konjević, Radomir M
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1672
AB  - A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.
T2  - Phyton-Annales Rei Botanicae
T1  - In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin
IS  - 1
VL  - 46
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1672
ER  - 

@article{
author = "Skorić, Marijana and Momčilović, Ivana and Krstić, Dijana and Maksimović, Vuk M and Konjević, Radomir M",
year = "2006",
abstract = "A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.",
journal = "Phyton-Annales Rei Botanicae",
title = "In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin",
number = "1",
volume = "46",
pages = "54",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1672"
}

Skorić, M., Momčilović, I., Krstić, D., Maksimović, V. M.,& Konjević, R. M.. (2006). In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin. in Phyton-Annales Rei Botanicae, 46(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1672

Skorić M, Momčilović I, Krstić D, Maksimović VM, Konjević RM. In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin. in Phyton-Annales Rei Botanicae. 2006;46(1):null-54.
https://hdl.handle.net/21.15107/rcub_ibiss_1672 .

Skorić, Marijana, Momčilović, Ivana, Krstić, Dijana, Maksimović, Vuk M, Konjević, Radomir M, "In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin" in Phyton-Annales Rei Botanicae, 46, no. 1 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1672 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Maksimović, Vuk M
AU  - Todorović, Slađana
AU  - Grubišić, Dragoljub V.
AU  - Konjević, Radomir M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1719
AB  - A wide range of sucrose, fructose, and glucose levels in culture media was tested in vitro in order to determine those that provide optimum growth, morphogenesis, and the production of secondary metabolites in Nepeta rtanjensis Diklic and Milojevic shoots. The effect of different concentrations of the carbohydrates in culture media on the internal carbohydrate status of N. rtanjensis shoots was also determined. Our results show that in vitro growth and development of N. rtanjensis, as well as nepetalactone accumulation, are significantly affected by both the type of carbohydrate and its concentration in the culture medium. Glucose proved to be the most efficient carbon and energy source.
T2  - Israel Journal of Plant Sciences
T1  - Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro
IS  - 2
VL  - 53
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1719
ER  - 

@article{
author = "Mišić, Danijela and Maksimović, Vuk M and Todorović, Slađana and Grubišić, Dragoljub V. and Konjević, Radomir M",
year = "2005",
abstract = "A wide range of sucrose, fructose, and glucose levels in culture media was tested in vitro in order to determine those that provide optimum growth, morphogenesis, and the production of secondary metabolites in Nepeta rtanjensis Diklic and Milojevic shoots. The effect of different concentrations of the carbohydrates in culture media on the internal carbohydrate status of N. rtanjensis shoots was also determined. Our results show that in vitro growth and development of N. rtanjensis, as well as nepetalactone accumulation, are significantly affected by both the type of carbohydrate and its concentration in the culture medium. Glucose proved to be the most efficient carbon and energy source.",
journal = "Israel Journal of Plant Sciences",
title = "Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro",
number = "2",
volume = "53",
pages = "108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1719"
}

Mišić, D., Maksimović, V. M., Todorović, S., Grubišić, D. V.,& Konjević, R. M.. (2005). Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro. in Israel Journal of Plant Sciences, 53(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1719

Mišić D, Maksimović VM, Todorović S, Grubišić DV, Konjević RM. Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro. in Israel Journal of Plant Sciences. 2005;53(2):null-108.
https://hdl.handle.net/21.15107/rcub_ibiss_1719 .

Mišić, Danijela, Maksimović, Vuk M, Todorović, Slađana, Grubišić, Dragoljub V., Konjević, Radomir M, "Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro" in Israel Journal of Plant Sciences, 53, no. 2 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1719 .