TY  - JOUR
AU  - Aleksić, Marija
AU  - Golić, Igor
AU  - Janković, Aleksandra
AU  - Čvoro, Aleksandra
AU  - Korać, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6529
AB  - We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.
PB  - London: Royal society publishing
T2  - Royal Society Open Science
T1  - ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats
VL  - 10
DO  - 10.1098/rsos.230109
SP  - 230109
ER  - 

@article{
author = "Aleksić, Marija and Golić, Igor and Janković, Aleksandra and Čvoro, Aleksandra and Korać, Aleksandra",
year = "2023",
abstract = "We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.",
publisher = "London: Royal society publishing",
journal = "Royal Society Open Science",
title = "ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats",
volume = "10",
doi = "10.1098/rsos.230109",
pages = "230109"
}

Aleksić, M., Golić, I., Janković, A., Čvoro, A.,& Korać, A.. (2023). ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats. in Royal Society Open Science
London: Royal society publishing., 10, 230109.
https://doi.org/10.1098/rsos.230109

Aleksić M, Golić I, Janković A, Čvoro A, Korać A. ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats. in Royal Society Open Science. 2023;10:230109.
doi:10.1098/rsos.230109 .

Aleksić, Marija, Golić, Igor, Janković, Aleksandra, Čvoro, Aleksandra, Korać, Aleksandra, "ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats" in Royal Society Open Science, 10 (2023):230109,
https://doi.org/10.1098/rsos.230109 . .

TY  - JOUR
AU  - Janković, Aleksandra
AU  - Ferreri, Carla
AU  - Filipović, Miloš
AU  - Ivanović-Burmazović, Ivana
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Korać, Aleksandra
AU  - Buzadžić, Biljana
AU  - Korać, Bato
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6211
AB  - Setting the correct ratio of superoxide anion (O2•-) and nitric oxide (•NO) radicals seems to be crucial in restoring disrupted redox signaling in diabetic skin and improvement of •NO physiological action for prevention and treatment of skin injuries in diabetes. In this study we examined the effects of L-arginine and manganese(II)-pentaazamacrocyclic superoxide dismutase (SOD) mimic - M40403 in diabetic rat skin. Following induction of diabetes by alloxan (blood glucose level ≥12 mMol l -1) non-diabetic and diabetic male Mill Hill hybrid hooded rats were divided into three subgroups: (i) control, and receiving: (ii) L-arginine, (iii) M40403. Treatment of diabetic animals started after diabetes induction and lasted for 7 days. Compared to control, lower cutaneous immuno-expression of endothelial NO synthase (eNOS), heme oxygenase 1 (HO1), manganese SOD (MnSOD) and glutathione peroxidase (GSH-Px), in parallel with increased NFE2-related factor 2 (Nrf2) and nitrotyrosine levels characterized diabetic skin. L-arginine and M40403 treatments normalized alloxan-induced increase in nitrotyrosine. This was accompanied by the improvement/restitution of eNOS and HO1 or MnSOD and GSH-Px protein expression levels in diabetic skin following L-arginine, i.e. SOD mimic treatments, respectively. The results indicate that L-arginine and M40403 stabilize redox balance in diabetic skin and suggest the underlying molecular mechanisms. Restitution of skin redox balance by L-arginine and M40403 may represent an effective strategy to ameliorate therapy of diabetic skin.
PB  - Taylor & Francis
T2  - Free Radical Research
T1  - Targeting the superoxide/nitric oxide ratio by L arginine and SOD mimic in rat diabetic skin
IS  - Sup1
VL  - 50
DO  - 10.1080/10715762.2016.1232483
SP  - S51
EP  - S63
ER  - 

@article{
author = "Janković, Aleksandra and Ferreri, Carla and Filipović, Miloš and Ivanović-Burmazović, Ivana and Stančić, Ana and Otašević, Vesna and Korać, Aleksandra and Buzadžić, Biljana and Korać, Bato",
year = "2016",
abstract = "Setting the correct ratio of superoxide anion (O2•-) and nitric oxide (•NO) radicals seems to be crucial in restoring disrupted redox signaling in diabetic skin and improvement of •NO physiological action for prevention and treatment of skin injuries in diabetes. In this study we examined the effects of L-arginine and manganese(II)-pentaazamacrocyclic superoxide dismutase (SOD) mimic - M40403 in diabetic rat skin. Following induction of diabetes by alloxan (blood glucose level ≥12 mMol l -1) non-diabetic and diabetic male Mill Hill hybrid hooded rats were divided into three subgroups: (i) control, and receiving: (ii) L-arginine, (iii) M40403. Treatment of diabetic animals started after diabetes induction and lasted for 7 days. Compared to control, lower cutaneous immuno-expression of endothelial NO synthase (eNOS), heme oxygenase 1 (HO1), manganese SOD (MnSOD) and glutathione peroxidase (GSH-Px), in parallel with increased NFE2-related factor 2 (Nrf2) and nitrotyrosine levels characterized diabetic skin. L-arginine and M40403 treatments normalized alloxan-induced increase in nitrotyrosine. This was accompanied by the improvement/restitution of eNOS and HO1 or MnSOD and GSH-Px protein expression levels in diabetic skin following L-arginine, i.e. SOD mimic treatments, respectively. The results indicate that L-arginine and M40403 stabilize redox balance in diabetic skin and suggest the underlying molecular mechanisms. Restitution of skin redox balance by L-arginine and M40403 may represent an effective strategy to ameliorate therapy of diabetic skin.",
publisher = "Taylor & Francis",
journal = "Free Radical Research",
title = "Targeting the superoxide/nitric oxide ratio by L arginine and SOD mimic in rat diabetic skin",
number = "Sup1",
volume = "50",
doi = "10.1080/10715762.2016.1232483",
pages = "S51-S63"
}

Janković, A., Ferreri, C., Filipović, M., Ivanović-Burmazović, I., Stančić, A., Otašević, V., Korać, A., Buzadžić, B.,& Korać, B.. (2016). Targeting the superoxide/nitric oxide ratio by L arginine and SOD mimic in rat diabetic skin. in Free Radical Research
Taylor & Francis., 50(Sup1), S51-S63.
https://doi.org/10.1080/10715762.2016.1232483

Janković A, Ferreri C, Filipović M, Ivanović-Burmazović I, Stančić A, Otašević V, Korać A, Buzadžić B, Korać B. Targeting the superoxide/nitric oxide ratio by L arginine and SOD mimic in rat diabetic skin. in Free Radical Research. 2016;50(Sup1):S51-S63.
doi:10.1080/10715762.2016.1232483 .

Janković, Aleksandra, Ferreri, Carla, Filipović, Miloš, Ivanović-Burmazović, Ivana, Stančić, Ana, Otašević, Vesna, Korać, Aleksandra, Buzadžić, Biljana, Korać, Bato, "Targeting the superoxide/nitric oxide ratio by L arginine and SOD mimic in rat diabetic skin" in Free Radical Research, 50, no. Sup1 (2016):S51-S63,
https://doi.org/10.1080/10715762.2016.1232483 . .

TY  - CONF
AU  - Surlan, L
AU  - Otašević, Vesna
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Vučetić, Milica
AU  - Stanković, V
AU  - Stojnić, J
AU  - Radunović, Nebojsa V
AU  - Tulić, Ivan
AU  - Korać, Bato
AU  - Korac, Aleksandra B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1002
C3  - Human Reproduction
T1  - Exosomes as bioactive messengers in a critical time for preimplantation embryos n an ultrastructural study
IS  - null
VL  - 28
SP  - 69
EP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1002
ER  - 

@conference{
author = "Surlan, L and Otašević, Vesna and Velicković, Ksenija D and Golić, Igor and Vučetić, Milica and Stanković, V and Stojnić, J and Radunović, Nebojsa V and Tulić, Ivan and Korać, Bato and Korac, Aleksandra B",
year = "2013",
journal = "Human Reproduction",
title = "Exosomes as bioactive messengers in a critical time for preimplantation embryos n an ultrastructural study",
number = "null",
volume = "28",
pages = "69-186",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1002"
}

Surlan, L., Otašević, V., Velicković, K. D., Golić, I., Vučetić, M., Stanković, V., Stojnić, J., Radunović, N. V., Tulić, I., Korać, B.,& Korac, A. B.. (2013). Exosomes as bioactive messengers in a critical time for preimplantation embryos n an ultrastructural study. in Human Reproduction, 28(null), 69-186.
https://hdl.handle.net/21.15107/rcub_ibiss_1002

Surlan L, Otašević V, Velicković KD, Golić I, Vučetić M, Stanković V, Stojnić J, Radunović NV, Tulić I, Korać B, Korac AB. Exosomes as bioactive messengers in a critical time for preimplantation embryos n an ultrastructural study. in Human Reproduction. 2013;28(null):69-186.
https://hdl.handle.net/21.15107/rcub_ibiss_1002 .

Surlan, L, Otašević, Vesna, Velicković, Ksenija D, Golić, Igor, Vučetić, Milica, Stanković, V, Stojnić, J, Radunović, Nebojsa V, Tulić, Ivan, Korać, Bato, Korac, Aleksandra B, "Exosomes as bioactive messengers in a critical time for preimplantation embryos n an ultrastructural study" in Human Reproduction, 28, no. null (2013):69-186,
https://hdl.handle.net/21.15107/rcub_ibiss_1002 .

TY  - JOUR
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Klepal, Waltraud
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korac, Aleksandra B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1030
AB  - The aim of this study was to investigate lipofuscin origin in brown adipocytes of hyperinsulinaemic rats and the possible role of lipid peroxidation and iron in this process. Ultrastructural examination revealed hyperinsulinaemia-induced enhancement in the lipofuscin production, accompanied by an increase of mitochondrial damage in brown adipocytes. Extensive fusions of lipid droplets and mitochondria with lysosomes were also observed. Confocal microscopy showed lipofuscin autofluorescence emission in brown adipose tissue (BAT) after excitation at 488 nm and 633 nm, particularly in the insulin-treated groups. The presence and distribution of lipid peroxidation product, 4-hydroxy-2-nonenal (4-HNE), in brown adipocytes was assessed by immunohistochemical examination revealing its higher content after treatment with insulin. The iron content was quantified by electron dispersive X-ray analysis (EDX) showing its higher content in the hyperinsulinaemic groups. The ultrastucture of the majority of lipofuscin granules suggests their mitochondrial origin, which was additionally confirmed by their co-localization with ATP synthase. In conclusion, our results suggest that increased lipofuscinogenesis in the brown adipocytes of hyperinsulinaemic rats is a consequence of lipid peroxidation, mitochondrial damage and iron accumulation.
T2  - Histology and Histopathology
T1  - The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron
IS  - 4
VL  - 28
SP  - 435
EP  - 503
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1030
ER  - 

@article{
author = "Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Klepal, Waltraud and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Buzadžić, Biljana J. and Korać, Bato and Korac, Aleksandra B",
year = "2013",
abstract = "The aim of this study was to investigate lipofuscin origin in brown adipocytes of hyperinsulinaemic rats and the possible role of lipid peroxidation and iron in this process. Ultrastructural examination revealed hyperinsulinaemia-induced enhancement in the lipofuscin production, accompanied by an increase of mitochondrial damage in brown adipocytes. Extensive fusions of lipid droplets and mitochondria with lysosomes were also observed. Confocal microscopy showed lipofuscin autofluorescence emission in brown adipose tissue (BAT) after excitation at 488 nm and 633 nm, particularly in the insulin-treated groups. The presence and distribution of lipid peroxidation product, 4-hydroxy-2-nonenal (4-HNE), in brown adipocytes was assessed by immunohistochemical examination revealing its higher content after treatment with insulin. The iron content was quantified by electron dispersive X-ray analysis (EDX) showing its higher content in the hyperinsulinaemic groups. The ultrastucture of the majority of lipofuscin granules suggests their mitochondrial origin, which was additionally confirmed by their co-localization with ATP synthase. In conclusion, our results suggest that increased lipofuscinogenesis in the brown adipocytes of hyperinsulinaemic rats is a consequence of lipid peroxidation, mitochondrial damage and iron accumulation.",
journal = "Histology and Histopathology",
title = "The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron",
number = "4",
volume = "28",
pages = "435-503",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1030"
}

Markelić, M. B., Velicković, K. D., Golić, I., Klepal, W., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Buzadžić, B. J., Korać, B.,& Korac, A. B.. (2013). The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron. in Histology and Histopathology, 28(4), 435-503.
https://hdl.handle.net/21.15107/rcub_ibiss_1030

Markelić MB, Velicković KD, Golić I, Klepal W, Otašević V, Stančić A, Janković A, Vučetić M, Buzadžić BJ, Korać B, Korac AB. The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron. in Histology and Histopathology. 2013;28(4):435-503.
https://hdl.handle.net/21.15107/rcub_ibiss_1030 .

Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Klepal, Waltraud, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Buzadžić, Biljana J., Korać, Bato, Korac, Aleksandra B, "The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron" in Histology and Histopathology, 28, no. 4 (2013):435-503,
https://hdl.handle.net/21.15107/rcub_ibiss_1030 .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Vučetić, Milica
AU  - Macanović, Biljana
AU  - Garalejić, Eliana
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Buzadžić, Biljana J.
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Markelić, Milica B
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1067
AB  - Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ((NO)-N-center dot), the number of MitoTracker (R) Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as (NO)-N-center dot might be involved in the observed effects of the mimic. Antioxid. Redox Signal. 18, 170-178.
T2  - Antioxidants & Redox Signaling
T1  - Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?
IS  - 2
VL  - 18
SP  - 23
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1067
ER  - 

@article{
author = "Otašević, Vesna and Korac, Aleksandra B and Vučetić, Milica and Macanović, Biljana and Garalejić, Eliana and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Buzadžić, Biljana J. and Stančić, Ana and Janković, Aleksandra and Velicković, Ksenija D and Golić, Igor and Markelić, Milica B and Korać, Bato",
year = "2013",
abstract = "Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ((NO)-N-center dot), the number of MitoTracker (R) Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as (NO)-N-center dot might be involved in the observed effects of the mimic. Antioxid. Redox Signal. 18, 170-178.",
journal = "Antioxidants & Redox Signaling",
title = "Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?",
number = "2",
volume = "18",
pages = "23-178",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1067"
}

Otašević, V., Korac, A. B., Vučetić, M., Macanović, B., Garalejić, E., Ivanović-Burmazović, I. S., Filipović, M. R., Buzadžić, B. J., Stančić, A., Janković, A., Velicković, K. D., Golić, I., Markelić, M. B.,& Korać, B.. (2013). Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?. in Antioxidants & Redox Signaling, 18(2), 23-178.
https://hdl.handle.net/21.15107/rcub_ibiss_1067

Otašević V, Korac AB, Vučetić M, Macanović B, Garalejić E, Ivanović-Burmazović IS, Filipović MR, Buzadžić BJ, Stančić A, Janković A, Velicković KD, Golić I, Markelić MB, Korać B. Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?. in Antioxidants & Redox Signaling. 2013;18(2):23-178.
https://hdl.handle.net/21.15107/rcub_ibiss_1067 .

Otašević, Vesna, Korac, Aleksandra B, Vučetić, Milica, Macanović, Biljana, Garalejić, Eliana, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Buzadžić, Biljana J., Stančić, Ana, Janković, Aleksandra, Velicković, Ksenija D, Golić, Igor, Markelić, Milica B, Korać, Bato, "Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?" in Antioxidants & Redox Signaling, 18, no. 2 (2013):23-178,
https://hdl.handle.net/21.15107/rcub_ibiss_1067 .

TY  - JOUR
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Korac, Aleksandra B
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/960
AB  - Hippocampal structural changes associated with diabetes-related cognitive impairments are well described, but their molecular background remained vague. We examined whether/how diabetes alters molecular basis of energy metabolism in hippocampus readily after diabetes onset, with special emphasis on its redox-sensitivity. To induce diabetes, adult Mill Hill hybrid hooded rats received a single alloxan dose (120 mg/kg). Both non-diabetic and diabetic groups were further divided in two subgroups receiving (i) or not (ii) superoxide dismutase (SOD) mimic, [Mn(II)(pyane)Cl-2] for 7 days, i.p. Treatment of the diabetic animals started after blood glucose level >= 12 mM. Diabetes decreased protein levels of oxidative phosphorylation components: complex III and ATP synthase. In contrast, protein amounts of glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, and hypoxia-inducible factor-1 alpha - the key regulator of energy metabolism in stress conditions, were higher in diabetic animals. Treatment with SOD mimic restored/increased the levels of oxidative phosphorylation components and returned hypoxia-inducible factor-1 alpha to control level, while diabetes-induced up-regulation of glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, was additionally stimulated. To conclude, our results provide insight into the earliest molecular changes of energy-producing pathways in diabetes that may account for structural/functional disturbance of hippocampus, seen during disease progression. Also, data suggest [Mn(II)(pyane)Cl-2] as potential therapeutic agent in cutting-edge approaches to threat this widespread metabolic disorder. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Brain Research Bulletin
T1  - Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic
IS  - null
VL  - 99
SP  - 153
EP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_960
ER  - 

@article{
author = "Stančić, Ana and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Korac, Aleksandra B and Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Buzadžić, Biljana J. and Korać, Bato",
year = "2013",
abstract = "Hippocampal structural changes associated with diabetes-related cognitive impairments are well described, but their molecular background remained vague. We examined whether/how diabetes alters molecular basis of energy metabolism in hippocampus readily after diabetes onset, with special emphasis on its redox-sensitivity. To induce diabetes, adult Mill Hill hybrid hooded rats received a single alloxan dose (120 mg/kg). Both non-diabetic and diabetic groups were further divided in two subgroups receiving (i) or not (ii) superoxide dismutase (SOD) mimic, [Mn(II)(pyane)Cl-2] for 7 days, i.p. Treatment of the diabetic animals started after blood glucose level >= 12 mM. Diabetes decreased protein levels of oxidative phosphorylation components: complex III and ATP synthase. In contrast, protein amounts of glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, and hypoxia-inducible factor-1 alpha - the key regulator of energy metabolism in stress conditions, were higher in diabetic animals. Treatment with SOD mimic restored/increased the levels of oxidative phosphorylation components and returned hypoxia-inducible factor-1 alpha to control level, while diabetes-induced up-regulation of glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, was additionally stimulated. To conclude, our results provide insight into the earliest molecular changes of energy-producing pathways in diabetes that may account for structural/functional disturbance of hippocampus, seen during disease progression. Also, data suggest [Mn(II)(pyane)Cl-2] as potential therapeutic agent in cutting-edge approaches to threat this widespread metabolic disorder. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Brain Research Bulletin",
title = "Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic",
number = "null",
volume = "99",
pages = "153-33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_960"
}

Stančić, A., Otašević, V., Janković, A., Vučetić, M., Ivanović-Burmazović, I. S., Filipović, M. R., Korac, A. B., Markelić, M. B., Velicković, K. D., Golić, I., Buzadžić, B. J.,& Korać, B.. (2013). Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic. in Brain Research Bulletin, 99(null), 153-33.
https://hdl.handle.net/21.15107/rcub_ibiss_960

Stančić A, Otašević V, Janković A, Vučetić M, Ivanović-Burmazović IS, Filipović MR, Korac AB, Markelić MB, Velicković KD, Golić I, Buzadžić BJ, Korać B. Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic. in Brain Research Bulletin. 2013;99(null):153-33.
https://hdl.handle.net/21.15107/rcub_ibiss_960 .

Stančić, Ana, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Korac, Aleksandra B, Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Buzadžić, Biljana J., Korać, Bato, "Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic" in Brain Research Bulletin, 99, no. null (2013):153-33,
https://hdl.handle.net/21.15107/rcub_ibiss_960 .

TY  - JOUR
AU  - Stančić, Ana
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/951
AB  - This study examined the molecular basis of energy-related regulatory mechanisms underlying metabolic recruitment of skeletal muscle during cold acclimation and possible involvement of the L-arginine/nitric oxide-producing pathway. Rats exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21 and 45 days were divided into three groups: untreated, L-arginine treated and N-omega-nitro-L-arginine methyl ester (L-NAME) treated. Compared with controls (22 +/- 1 degrees C), there was an initial increase in the protein level of 5'-AMP-activated protein kinase alpha (day 1), followed by an increase in peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) and peroxisome proliferator-activated receptors (PPARs): PPAR alpha and PPAR gamma from day 1 and PPAR delta from day 7 of cold acclimation. Activation of the PGC-1 alpha/PPAR transcription program was accompanied by increased protein expression of the key metabolic enzymes in beta-oxidation, the tricarboxylic acid cycle and oxidative phosphorylation, with the exceptions in complex I (no changes) and ATP synthase (decreased at day 1). Cold did not affect hexokinase and GAPDH protein levels, but increased lactate dehydrogenase activity compared with controls (1-45 days). L-arginine sustained, accelerated and/or intensified cold-induced molecular remodeling throughout cold acclimation. L-NAME exerted phase-dependent effects: similar to L-arginine in early cold acclimation and opposite after prolonged cold exposure (from day 21). It seems that upregulation of the PGC-1 alpha/PPAR transcription program early during cold acclimation triggers the molecular recruitment of skeletal muscle underlying the shift to more oxidative metabolism during prolonged cold acclimation. Our results suggest that nitric oxide has a role in maintaining the skeletal muscle oxidative phenotype in late cold acclimation but question its role early in cold acclimation.
T2  - Journal of Experimental Biology
T1  - Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation
IS  - 22
VL  - 216
SP  - 73
EP  - 4241
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_951
ER  - 

@article{
author = "Stančić, Ana and Buzadžić, Biljana J. and Korac, Aleksandra B and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Korać, Bato",
year = "2013",
abstract = "This study examined the molecular basis of energy-related regulatory mechanisms underlying metabolic recruitment of skeletal muscle during cold acclimation and possible involvement of the L-arginine/nitric oxide-producing pathway. Rats exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21 and 45 days were divided into three groups: untreated, L-arginine treated and N-omega-nitro-L-arginine methyl ester (L-NAME) treated. Compared with controls (22 +/- 1 degrees C), there was an initial increase in the protein level of 5'-AMP-activated protein kinase alpha (day 1), followed by an increase in peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) and peroxisome proliferator-activated receptors (PPARs): PPAR alpha and PPAR gamma from day 1 and PPAR delta from day 7 of cold acclimation. Activation of the PGC-1 alpha/PPAR transcription program was accompanied by increased protein expression of the key metabolic enzymes in beta-oxidation, the tricarboxylic acid cycle and oxidative phosphorylation, with the exceptions in complex I (no changes) and ATP synthase (decreased at day 1). Cold did not affect hexokinase and GAPDH protein levels, but increased lactate dehydrogenase activity compared with controls (1-45 days). L-arginine sustained, accelerated and/or intensified cold-induced molecular remodeling throughout cold acclimation. L-NAME exerted phase-dependent effects: similar to L-arginine in early cold acclimation and opposite after prolonged cold exposure (from day 21). It seems that upregulation of the PGC-1 alpha/PPAR transcription program early during cold acclimation triggers the molecular recruitment of skeletal muscle underlying the shift to more oxidative metabolism during prolonged cold acclimation. Our results suggest that nitric oxide has a role in maintaining the skeletal muscle oxidative phenotype in late cold acclimation but question its role early in cold acclimation.",
journal = "Journal of Experimental Biology",
title = "Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation",
number = "22",
volume = "216",
pages = "73-4241",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_951"
}

Stančić, A., Buzadžić, B. J., Korac, A. B., Otašević, V., Janković, A., Vučetić, M., Markelić, M. B., Velicković, K. D., Golić, I.,& Korać, B.. (2013). Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation. in Journal of Experimental Biology, 216(22), 73-4241.
https://hdl.handle.net/21.15107/rcub_ibiss_951

Stančić A, Buzadžić BJ, Korac AB, Otašević V, Janković A, Vučetić M, Markelić MB, Velicković KD, Golić I, Korać B. Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation. in Journal of Experimental Biology. 2013;216(22):73-4241.
https://hdl.handle.net/21.15107/rcub_ibiss_951 .

Stančić, Ana, Buzadžić, Biljana J., Korac, Aleksandra B, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Korać, Bato, "Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation" in Journal of Experimental Biology, 216, no. 22 (2013):73-4241,
https://hdl.handle.net/21.15107/rcub_ibiss_951 .

TY  - JOUR
AU  - Vučetić, Milica
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Korac, Aleksandra B
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Buzadžić, Biljana J.
AU  - Storey, Kenneth B
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/944
AB  - Any alteration in oxidative metabolism is coupled with a corresponding response by an antioxidant defense (AD) in appropriate subcellular compartments. Seasonal hibernators pass through circannual metabolic adaptations that allow them to either maintain euthermy (cold acclimation) or enter winter torpor with body temperature falling to low values. The present study aimed to investigate the corresponding pattern of AD enzyme protein expressions associated with these strategies in the main tissues involved in whole animal energy homeostasis: brown and white adipose tissues (BAT and WAT, respectively), liver, and skeletal muscle. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 +/- 1 C) and then divided into two groups: (1) animals fell into torpor (hibernating group) and (2) animals stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group). We examined the effects of cold acclimation and hibernation on the tissue-dependent protein expression of four enzymes which catalyze the two-step detoxification of superoxide to water: superoxide dismutase 1 and 2 (SOD 1 and 2), catalase (CAT), and glutathione peroxidase (GSH-Px). The results showed that hibernation induced an increase of AD enzyme protein expressions in BAT and skeletal muscle. However, AD enzyme contents in liver were largely unaffected during torpor. Under these conditions, different WAT depots responded by elevating the amounts of specific enzymes, as follows: SOD 1 in retroperitoneal WAT, GSH-Px in gonadal WAT, and CAT in subcutaneous WAT. Similar perturbations of AD enzymes contents were seen in all tissues during cold acclimation, often in a time-dependent manner. It can be concluded that BAT and muscle AD capacity undergo the most dramatic changes during both cold acclimation and hibernation, while liver is relatively unaffected by either condition. Additionally, this study provides a basis for further metabolic study that will illuminate the causes of these tissue-specific AD responses, particularly the novel finding of distinct responses by different WAT depots in hibernators. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Free Radical Biology and Medicine
T1  - The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update
IS  - null
VL  - 65
SP  - 47
EP  - 924
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_944
ER  - 

@article{
author = "Vučetić, Milica and Stančić, Ana and Otašević, Vesna and Janković, Aleksandra and Korac, Aleksandra B and Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Buzadžić, Biljana J. and Storey, Kenneth B and Korać, Bato",
year = "2013",
abstract = "Any alteration in oxidative metabolism is coupled with a corresponding response by an antioxidant defense (AD) in appropriate subcellular compartments. Seasonal hibernators pass through circannual metabolic adaptations that allow them to either maintain euthermy (cold acclimation) or enter winter torpor with body temperature falling to low values. The present study aimed to investigate the corresponding pattern of AD enzyme protein expressions associated with these strategies in the main tissues involved in whole animal energy homeostasis: brown and white adipose tissues (BAT and WAT, respectively), liver, and skeletal muscle. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 +/- 1 C) and then divided into two groups: (1) animals fell into torpor (hibernating group) and (2) animals stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group). We examined the effects of cold acclimation and hibernation on the tissue-dependent protein expression of four enzymes which catalyze the two-step detoxification of superoxide to water: superoxide dismutase 1 and 2 (SOD 1 and 2), catalase (CAT), and glutathione peroxidase (GSH-Px). The results showed that hibernation induced an increase of AD enzyme protein expressions in BAT and skeletal muscle. However, AD enzyme contents in liver were largely unaffected during torpor. Under these conditions, different WAT depots responded by elevating the amounts of specific enzymes, as follows: SOD 1 in retroperitoneal WAT, GSH-Px in gonadal WAT, and CAT in subcutaneous WAT. Similar perturbations of AD enzymes contents were seen in all tissues during cold acclimation, often in a time-dependent manner. It can be concluded that BAT and muscle AD capacity undergo the most dramatic changes during both cold acclimation and hibernation, while liver is relatively unaffected by either condition. Additionally, this study provides a basis for further metabolic study that will illuminate the causes of these tissue-specific AD responses, particularly the novel finding of distinct responses by different WAT depots in hibernators. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Free Radical Biology and Medicine",
title = "The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update",
number = "null",
volume = "65",
pages = "47-924",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_944"
}

Vučetić, M., Stančić, A., Otašević, V., Janković, A., Korac, A. B., Markelić, M. B., Velicković, K. D., Golić, I., Buzadžić, B. J., Storey, K. B.,& Korać, B.. (2013). The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update. in Free Radical Biology and Medicine, 65(null), 47-924.
https://hdl.handle.net/21.15107/rcub_ibiss_944

Vučetić M, Stančić A, Otašević V, Janković A, Korac AB, Markelić MB, Velicković KD, Golić I, Buzadžić BJ, Storey KB, Korać B. The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update. in Free Radical Biology and Medicine. 2013;65(null):47-924.
https://hdl.handle.net/21.15107/rcub_ibiss_944 .

Vučetić, Milica, Stančić, Ana, Otašević, Vesna, Janković, Aleksandra, Korac, Aleksandra B, Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Buzadžić, Biljana J., Storey, Kenneth B, Korać, Bato, "The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update" in Free Radical Biology and Medicine, 65, no. null (2013):47-924,
https://hdl.handle.net/21.15107/rcub_ibiss_944 .

TY  - JOUR
AU  - Vučetić, Milica
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Markelić, Milica B
AU  - Golić, Igor
AU  - Velicković, Ksenija D
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1148
AB  - In this study, the effects of l-arginine-nitric-oxide ((NO)-N-a (TM))-producing pathway on protein content of ubiquitin, as an important component of ubiquitin-proteasome system for protein removal, were investigated. We showed that l-arginine markedly decreased ubiquitin protein content in interscapular brown adipose tissue, both in thermogenic inactive (at room temperature) and thermogenic active (on cold) states; while in l-NAME-treated groups this effect was abolished. This result suggests that nitric oxide ((NO)-N-a (TM)), besides well established roles, is involved in this aspect of structure remodeling, as well.
T2  - Molecular and Cellular Biochemistry
T1  - Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot
IS  - 1-2
VL  - 368
SP  - 503
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1148
ER  - 

@article{
author = "Vučetić, Milica and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Markelić, Milica B and Golić, Igor and Velicković, Ksenija D and Buzadžić, Biljana J. and Korac, Aleksandra B and Korać, Bato",
year = "2012",
abstract = "In this study, the effects of l-arginine-nitric-oxide ((NO)-N-a (TM))-producing pathway on protein content of ubiquitin, as an important component of ubiquitin-proteasome system for protein removal, were investigated. We showed that l-arginine markedly decreased ubiquitin protein content in interscapular brown adipose tissue, both in thermogenic inactive (at room temperature) and thermogenic active (on cold) states; while in l-NAME-treated groups this effect was abolished. This result suggests that nitric oxide ((NO)-N-a (TM)), besides well established roles, is involved in this aspect of structure remodeling, as well.",
journal = "Molecular and Cellular Biochemistry",
title = "Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot",
number = "1-2",
volume = "368",
pages = "503-193",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1148"
}

Vučetić, M., Otašević, V., Stančić, A., Janković, A., Markelić, M. B., Golić, I., Velicković, K. D., Buzadžić, B. J., Korac, A. B.,& Korać, B.. (2012). Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot. in Molecular and Cellular Biochemistry, 368(1-2), 503-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1148

Vučetić M, Otašević V, Stančić A, Janković A, Markelić MB, Golić I, Velicković KD, Buzadžić BJ, Korac AB, Korać B. Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot. in Molecular and Cellular Biochemistry. 2012;368(1-2):503-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1148 .

Vučetić, Milica, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Markelić, Milica B, Golić, Igor, Velicković, Ksenija D, Buzadžić, Biljana J., Korac, Aleksandra B, Korać, Bato, "Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot" in Molecular and Cellular Biochemistry, 368, no. 1-2 (2012):503-193,
https://hdl.handle.net/21.15107/rcub_ibiss_1148 .

TY  - JOUR
AU  - Jović, Miomir Đ
AU  - Stančić, Ana
AU  - Nenadić, Dragan
AU  - Cekić, Olivera
AU  - Nezić, Dusko G
AU  - Milojević, Predrag S
AU  - Micović, Slobodan V
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1138
AB  - Background/Aims: Study elucidates and compares the mitochondrial bioenergetic-related molecular basis of sevoflurane and propofol cardioprotection during aortic valve replacement surgery due to aortic valve stenosis. Methods: Twenty-two patients were prospectively randomized in two groups regarding the anesthetic regime: sevoflurane and propofol. Hemodynamic parameters, biomarkers of cardiac injury and brain natriuretic peptide (BNP) were measured preoperatively and postoperatively. In tissue samples, taken from the interventricular septum, key mitochondrial molecules were determined by Western blot, real time PCR, as well as confocal microscopy and immunohisto- and immunocyto-chemical analysis. Results: The protein levels of cytochrome c oxidase and ATP synthase were higher in sevoflurane than in propofol group. Nevertheless, cytochrome c protein content was higher in propofol than sevoflurane receiving patients. Propofol group also showed higher protein level of connexin 43 (Cx43) than sevoflurane group. Besides, immunogold analysis showed its mitochondrial localization. The mRNA level of mtDNA and uncoupling protein (UCP2) were higher in propofol than sevoflurane patients, as well. On the other hand, there were no significant differences between groups in hemodynamic assessment, intensive care unit length of stay, troponin I and BNP level. Conclusions: Our data indicate that sevoflurane and propofol lead to cardiac protection via different mitochondrially related molecular mechanisms. It appears that sevoflurane acts regulating cytochrome c oxidase and ATP synthase, while the effects of propofol occur through regulation of cytochrome c, Cx43, mtDNA transcription and UCP2. Copyright (C) 2012 S. Karger AG, Basel
T2  - Cellular Physiology and Biochemistry
T1  - Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass
IS  - 1-2
VL  - 29
SP  - 973
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1138
ER  - 

@article{
author = "Jović, Miomir Đ and Stančić, Ana and Nenadić, Dragan and Cekić, Olivera and Nezić, Dusko G and Milojević, Predrag S and Micović, Slobodan V and Buzadžić, Biljana J. and Korac, Aleksandra B and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Velicković, Ksenija D and Golić, Igor and Korać, Bato",
year = "2012",
abstract = "Background/Aims: Study elucidates and compares the mitochondrial bioenergetic-related molecular basis of sevoflurane and propofol cardioprotection during aortic valve replacement surgery due to aortic valve stenosis. Methods: Twenty-two patients were prospectively randomized in two groups regarding the anesthetic regime: sevoflurane and propofol. Hemodynamic parameters, biomarkers of cardiac injury and brain natriuretic peptide (BNP) were measured preoperatively and postoperatively. In tissue samples, taken from the interventricular septum, key mitochondrial molecules were determined by Western blot, real time PCR, as well as confocal microscopy and immunohisto- and immunocyto-chemical analysis. Results: The protein levels of cytochrome c oxidase and ATP synthase were higher in sevoflurane than in propofol group. Nevertheless, cytochrome c protein content was higher in propofol than sevoflurane receiving patients. Propofol group also showed higher protein level of connexin 43 (Cx43) than sevoflurane group. Besides, immunogold analysis showed its mitochondrial localization. The mRNA level of mtDNA and uncoupling protein (UCP2) were higher in propofol than sevoflurane patients, as well. On the other hand, there were no significant differences between groups in hemodynamic assessment, intensive care unit length of stay, troponin I and BNP level. Conclusions: Our data indicate that sevoflurane and propofol lead to cardiac protection via different mitochondrially related molecular mechanisms. It appears that sevoflurane acts regulating cytochrome c oxidase and ATP synthase, while the effects of propofol occur through regulation of cytochrome c, Cx43, mtDNA transcription and UCP2. Copyright (C) 2012 S. Karger AG, Basel",
journal = "Cellular Physiology and Biochemistry",
title = "Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass",
number = "1-2",
volume = "29",
pages = "973-142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1138"
}

Jović, M. Đ., Stančić, A., Nenadić, D., Cekić, O., Nezić, D. G., Milojević, P. S., Micović, S. V., Buzadžić, B. J., Korac, A. B., Otašević, V., Janković, A., Vučetić, M., Velicković, K. D., Golić, I.,& Korać, B.. (2012). Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass. in Cellular Physiology and Biochemistry, 29(1-2), 973-142.
https://hdl.handle.net/21.15107/rcub_ibiss_1138

Jović MĐ, Stančić A, Nenadić D, Cekić O, Nezić DG, Milojević PS, Micović SV, Buzadžić BJ, Korac AB, Otašević V, Janković A, Vučetić M, Velicković KD, Golić I, Korać B. Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass. in Cellular Physiology and Biochemistry. 2012;29(1-2):973-142.
https://hdl.handle.net/21.15107/rcub_ibiss_1138 .

Jović, Miomir Đ, Stančić, Ana, Nenadić, Dragan, Cekić, Olivera, Nezić, Dusko G, Milojević, Predrag S, Micović, Slobodan V, Buzadžić, Biljana J., Korac, Aleksandra B, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Velicković, Ksenija D, Golić, Igor, Korać, Bato, "Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass" in Cellular Physiology and Biochemistry, 29, no. 1-2 (2012):973-142,
https://hdl.handle.net/21.15107/rcub_ibiss_1138 .

TY  - CONF
AU  - Vučetić, Milica
AU  - Stančić, Ana
AU  - Filipović, Milos R
AU  - Ivanović-Burmazović, Ivana S
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Janković, Aleksandra
AU  - Buzadžić, Biljana J.
AU  - Velicković, Ksenija D
AU  - Markelić, Milica B
AU  - Golić, Igor
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1137
C3  - European Journal of Clinical Investigation
T1  - The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats
IS  - null
VL  - 42
SP  - 969
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1137
ER  - 

@conference{
author = "Vučetić, Milica and Stančić, Ana and Filipović, Milos R and Ivanović-Burmazović, Ivana S and Otašević, Vesna and Korac, Aleksandra B and Janković, Aleksandra and Buzadžić, Biljana J. and Velicković, Ksenija D and Markelić, Milica B and Golić, Igor and Korać, Bato",
year = "2012",
journal = "European Journal of Clinical Investigation",
title = "The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats",
number = "null",
volume = "42",
pages = "969-76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1137"
}

Vučetić, M., Stančić, A., Filipović, M. R., Ivanović-Burmazović, I. S., Otašević, V., Korac, A. B., Janković, A., Buzadžić, B. J., Velicković, K. D., Markelić, M. B., Golić, I.,& Korać, B.. (2012). The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats. in European Journal of Clinical Investigation, 42(null), 969-76.
https://hdl.handle.net/21.15107/rcub_ibiss_1137

Vučetić M, Stančić A, Filipović MR, Ivanović-Burmazović IS, Otašević V, Korac AB, Janković A, Buzadžić BJ, Velicković KD, Markelić MB, Golić I, Korać B. The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats. in European Journal of Clinical Investigation. 2012;42(null):969-76.
https://hdl.handle.net/21.15107/rcub_ibiss_1137 .

Vučetić, Milica, Stančić, Ana, Filipović, Milos R, Ivanović-Burmazović, Ivana S, Otašević, Vesna, Korac, Aleksandra B, Janković, Aleksandra, Buzadžić, Biljana J., Velicković, Ksenija D, Markelić, Milica B, Golić, Igor, Korać, Bato, "The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats" in European Journal of Clinical Investigation, 42, no. null (2012):969-76,
https://hdl.handle.net/21.15107/rcub_ibiss_1137 .

TY  - JOUR
AU  - Stančić, Ana
AU  - Rasić-Milutinović, Zorica R
AU  - Perunicić-Peković, Gordana B
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1189
AB  - Diabetes and renal insufficiency are interrelated metabolic disorders closely associated with redox homeostasis disturbances. The aim of this study was to compare the activity of copper zinc,superoxide dismutase (CuZnSOD) in the erythrocytes of hypertensive diabetic patients with or without renal insufficiency with normal healthy control subjects. In both groups of diabetic patients, blood glucose level and the content of glycosylated hemoglobin (HbA(lc)) were higher than in the control group. However, CuZnSOD activity was significantly higher than control only in hypertensive diabetic patients with renal insufficiency. Our results suggest that disturbances in superoxide homeostasis do correlate with long-term complication in diabetes, i.e. diabetic renal insufficiency and hypertension.
T2  - Indian Journal of Biochemistry & Biophysics
T1  - Relation of CuZnSOD activity with renal insufficiency in hypertensive diabetic patients
IS  - 2
VL  - 49
EP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1189
ER  - 

@article{
author = "Stančić, Ana and Rasić-Milutinović, Zorica R and Perunicić-Peković, Gordana B and Buzadžić, Biljana J. and Korac, Aleksandra B and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Korać, Bato",
year = "2012",
abstract = "Diabetes and renal insufficiency are interrelated metabolic disorders closely associated with redox homeostasis disturbances. The aim of this study was to compare the activity of copper zinc,superoxide dismutase (CuZnSOD) in the erythrocytes of hypertensive diabetic patients with or without renal insufficiency with normal healthy control subjects. In both groups of diabetic patients, blood glucose level and the content of glycosylated hemoglobin (HbA(lc)) were higher than in the control group. However, CuZnSOD activity was significantly higher than control only in hypertensive diabetic patients with renal insufficiency. Our results suggest that disturbances in superoxide homeostasis do correlate with long-term complication in diabetes, i.e. diabetic renal insufficiency and hypertension.",
journal = "Indian Journal of Biochemistry & Biophysics",
title = "Relation of CuZnSOD activity with renal insufficiency in hypertensive diabetic patients",
number = "2",
volume = "49",
pages = "100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1189"
}

Stančić, A., Rasić-Milutinović, Z. R., Perunicić-Peković, G. B., Buzadžić, B. J., Korac, A. B., Otašević, V., Janković, A., Vučetić, M.,& Korać, B.. (2012). Relation of CuZnSOD activity with renal insufficiency in hypertensive diabetic patients. in Indian Journal of Biochemistry & Biophysics, 49(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1189

Stančić A, Rasić-Milutinović ZR, Perunicić-Peković GB, Buzadžić BJ, Korac AB, Otašević V, Janković A, Vučetić M, Korać B. Relation of CuZnSOD activity with renal insufficiency in hypertensive diabetic patients. in Indian Journal of Biochemistry & Biophysics. 2012;49(2):null-100.
https://hdl.handle.net/21.15107/rcub_ibiss_1189 .

Stančić, Ana, Rasić-Milutinović, Zorica R, Perunicić-Peković, Gordana B, Buzadžić, Biljana J., Korac, Aleksandra B, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Korać, Bato, "Relation of CuZnSOD activity with renal insufficiency in hypertensive diabetic patients" in Indian Journal of Biochemistry & Biophysics, 49, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1189 .

TY  - CONF
AU  - Macanović, Biljana
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Vučetić, Milica
AU  - Garalejić, Eliana
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Buzadžić, Biljana J.
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Markelić, Milica B
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1212
C3  - Human Reproduction
T1  - Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO
IS  - null
VL  - 27
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1212
ER  - 

@conference{
author = "Macanović, Biljana and Otašević, Vesna and Korac, Aleksandra B and Vučetić, Milica and Garalejić, Eliana and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Buzadžić, Biljana J. and Stančić, Ana and Janković, Aleksandra and Velicković, Ksenija D and Golić, Igor and Markelić, Milica B and Korać, Bato",
year = "2012",
journal = "Human Reproduction",
title = "Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO",
number = "null",
volume = "27",
pages = "na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1212"
}

Macanović, B., Otašević, V., Korac, A. B., Vučetić, M., Garalejić, E., Ivanović-Burmazović, I. S., Filipović, M. R., Buzadžić, B. J., Stančić, A., Janković, A., Velicković, K. D., Golić, I., Markelić, M. B.,& Korać, B.. (2012). Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO. in Human Reproduction, 27(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1212

Macanović B, Otašević V, Korac AB, Vučetić M, Garalejić E, Ivanović-Burmazović IS, Filipović MR, Buzadžić BJ, Stančić A, Janković A, Velicković KD, Golić I, Markelić MB, Korać B. Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO. in Human Reproduction. 2012;27(null):null-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1212 .

Macanović, Biljana, Otašević, Vesna, Korac, Aleksandra B, Vučetić, Milica, Garalejić, Eliana, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Buzadžić, Biljana J., Stančić, Ana, Janković, Aleksandra, Velicković, Ksenija D, Golić, Igor, Markelić, Milica B, Korać, Bato, "Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO" in Human Reproduction, 27, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1212 .

TY  - JOUR
AU  - Vučetić, Milica
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Markelić, Milica B
AU  - Golić, Igor
AU  - Velicković, Ksenija D
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1243
AB  - Background: Brown adipose tissue thermogenic program includes complex molecular and structural changes. However, energetic aspects of this process are poorly depicted. Methods: We investigated time-dependent reprogramming of interscapular brown adipose tissue (IBAT) energy metabolism during cold-acclimation, as well as the effects of nitric oxide ((center dot)NO) on those changes. Rats were exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21. and 45 days, and divided into three groups: control, treated with L-arginine, and treated with N(omega)-nitro-L-arginine methyl ester (L-NAME). Results: In the early phase of cold-acclimation (up to 7 days), the protein levels of all metabolic parameters and oxidative phosphorylation components were below the control. However, metabolic parameters and respiratory chain components entered a new homeostatic level in the late phase of cold-acclimation. These changes were accompanied with increased protein levels of phospho-AMP-dependent protein kinase-alpha (phospho-AMPK alpha) on the first day of cold-acclimation, and hypoxia-inducible factor-1 alpha (HIF-1 alpha) throughout early cold-acclimation. L-arginine positively affected protein expression of enzymes involved in glucose metabolism and beta-oxidation of fatty acids in the early phase of cold-acclimation, and oxidative phosphorylation components throughout cold-acclimation. In contrast, L-NAME had the opposite effects. Conclusion: Results suggest that IBAT structural remodeling is followed by energy metabolism reprogramming, which control might be orchestrated by the action of AMPK alpha and HIF-1 alpha. Data also indicated the involvement of L-arginine-(center dot)NO in the regulation of IBAT metabolism. General significance: Results obtained in this study might be of great importance for elucidating regulatory pathways governing energy metabolism in both physiological and pathophysiological states. (C) 2011 Elsevier B.V. All rights reserved.
T2  - Biochimica et Biophysica Acta-General Subjects
T1  - Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha
IS  - 12
VL  - 1810
EP  - 1261
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1243
ER  - 

@article{
author = "Vučetić, Milica and Otašević, Vesna and Korac, Aleksandra B and Stančić, Ana and Janković, Aleksandra and Markelić, Milica B and Golić, Igor and Velicković, Ksenija D and Buzadžić, Biljana J. and Korać, Bato",
year = "2011",
abstract = "Background: Brown adipose tissue thermogenic program includes complex molecular and structural changes. However, energetic aspects of this process are poorly depicted. Methods: We investigated time-dependent reprogramming of interscapular brown adipose tissue (IBAT) energy metabolism during cold-acclimation, as well as the effects of nitric oxide ((center dot)NO) on those changes. Rats were exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21. and 45 days, and divided into three groups: control, treated with L-arginine, and treated with N(omega)-nitro-L-arginine methyl ester (L-NAME). Results: In the early phase of cold-acclimation (up to 7 days), the protein levels of all metabolic parameters and oxidative phosphorylation components were below the control. However, metabolic parameters and respiratory chain components entered a new homeostatic level in the late phase of cold-acclimation. These changes were accompanied with increased protein levels of phospho-AMP-dependent protein kinase-alpha (phospho-AMPK alpha) on the first day of cold-acclimation, and hypoxia-inducible factor-1 alpha (HIF-1 alpha) throughout early cold-acclimation. L-arginine positively affected protein expression of enzymes involved in glucose metabolism and beta-oxidation of fatty acids in the early phase of cold-acclimation, and oxidative phosphorylation components throughout cold-acclimation. In contrast, L-NAME had the opposite effects. Conclusion: Results suggest that IBAT structural remodeling is followed by energy metabolism reprogramming, which control might be orchestrated by the action of AMPK alpha and HIF-1 alpha. Data also indicated the involvement of L-arginine-(center dot)NO in the regulation of IBAT metabolism. General significance: Results obtained in this study might be of great importance for elucidating regulatory pathways governing energy metabolism in both physiological and pathophysiological states. (C) 2011 Elsevier B.V. All rights reserved.",
journal = "Biochimica et Biophysica Acta-General Subjects",
title = "Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha",
number = "12",
volume = "1810",
pages = "1261",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1243"
}

Vučetić, M., Otašević, V., Korac, A. B., Stančić, A., Janković, A., Markelić, M. B., Golić, I., Velicković, K. D., Buzadžić, B. J.,& Korać, B.. (2011). Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha. in Biochimica et Biophysica Acta-General Subjects, 1810(12).
https://hdl.handle.net/21.15107/rcub_ibiss_1243

Vučetić M, Otašević V, Korac AB, Stančić A, Janković A, Markelić MB, Golić I, Velicković KD, Buzadžić BJ, Korać B. Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha. in Biochimica et Biophysica Acta-General Subjects. 2011;1810(12):null-1261.
https://hdl.handle.net/21.15107/rcub_ibiss_1243 .

Vučetić, Milica, Otašević, Vesna, Korac, Aleksandra B, Stančić, Ana, Janković, Aleksandra, Markelić, Milica B, Golić, Igor, Velicković, Ksenija D, Buzadžić, Biljana J., Korać, Bato, "Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha" in Biochimica et Biophysica Acta-General Subjects, 1810, no. 12 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1243 .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Korać, Bato
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1281
AB  - Study elucidates molecular mechanisms underlying activation of catalase and glutathione peroxidase (GSH-Px) during cold-exposure by examining time-dependent changes in their mRNA, protein expression and activity, in interscapular brown adipose tissue (IBAT) of rats kept at room temperature (22 +/- 1 degrees C), or subjected to cold (4 +/- 1 degrees C) for 1, 3, 7, 12, 21 and 45 days. To examine involvement of nitric oxide (*NO) in catalase and GSH-Px regulation, rats were treated with L-arginine or N((1))-nitro-L-arginine-methyl ester (L-NAME). In all groups, cold increased catalase mRNA, protein expression and activity from day 3 to day 45 of acclimation. However, cold-acclimation increased GSH-Px mRNA content on day 3, protein content from day 1 to day 45 and activity no sooner than on day 45. At room temperature, 21- and 45-day L-arginine and L-NAME supplementation increased catalase and GSH-Px mRNA expression, but decreased their activity. Concomitantly, catalase protein content decreased, while GSH-Px protein expression increased compared to control. Data show that cold is dominant stimulus that determines IBAT catalase and GSH-Px responses, by setting tissue metabolic state. In thermogenic-active tissue, catalase is regulated on transcriptional level, while GSH-Px is regulated post-translationally. On the other side, it was shown that *NO affects catalase and GSH-Px only in thermogenic-inactive tissue. (C) 2011 Elsevier Ltd. All rights reserved.
T2  - Journal of Thermal Biology
T1  - Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures
IS  - 5
VL  - 36
EP  - 276
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1281
ER  - 

@article{
author = "Otašević, Vesna and Buzadžić, Biljana J. and Korac, Aleksandra B and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Korać, Bato",
year = "2011",
abstract = "Study elucidates molecular mechanisms underlying activation of catalase and glutathione peroxidase (GSH-Px) during cold-exposure by examining time-dependent changes in their mRNA, protein expression and activity, in interscapular brown adipose tissue (IBAT) of rats kept at room temperature (22 +/- 1 degrees C), or subjected to cold (4 +/- 1 degrees C) for 1, 3, 7, 12, 21 and 45 days. To examine involvement of nitric oxide (*NO) in catalase and GSH-Px regulation, rats were treated with L-arginine or N((1))-nitro-L-arginine-methyl ester (L-NAME). In all groups, cold increased catalase mRNA, protein expression and activity from day 3 to day 45 of acclimation. However, cold-acclimation increased GSH-Px mRNA content on day 3, protein content from day 1 to day 45 and activity no sooner than on day 45. At room temperature, 21- and 45-day L-arginine and L-NAME supplementation increased catalase and GSH-Px mRNA expression, but decreased their activity. Concomitantly, catalase protein content decreased, while GSH-Px protein expression increased compared to control. Data show that cold is dominant stimulus that determines IBAT catalase and GSH-Px responses, by setting tissue metabolic state. In thermogenic-active tissue, catalase is regulated on transcriptional level, while GSH-Px is regulated post-translationally. On the other side, it was shown that *NO affects catalase and GSH-Px only in thermogenic-inactive tissue. (C) 2011 Elsevier Ltd. All rights reserved.",
journal = "Journal of Thermal Biology",
title = "Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures",
number = "5",
volume = "36",
pages = "276",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1281"
}

Otašević, V., Buzadžić, B. J., Korac, A. B., Stančić, A., Janković, A., Vučetić, M.,& Korać, B.. (2011). Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures. in Journal of Thermal Biology, 36(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1281

Otašević V, Buzadžić BJ, Korac AB, Stančić A, Janković A, Vučetić M, Korać B. Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures. in Journal of Thermal Biology. 2011;36(5):null-276.
https://hdl.handle.net/21.15107/rcub_ibiss_1281 .

Otašević, Vesna, Buzadžić, Biljana J., Korac, Aleksandra B, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Korać, Bato, "Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures" in Journal of Thermal Biology, 36, no. 5 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1281 .

TY  - JOUR
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korac, Aleksandra B
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1324
AB  - The aim of the present study was to investigate whether hyperinsulinaemia, which frequently precedes insulin resistance syndrome (obesity, diabetes), induces apoptosis of endothelial cells (ECs) in brown adipose tissue (BAT) and causes BAT atrophy and also, to investigate the possible mechanisms underlying ECs death. In order to induce hyperinsulinaemia, adult male rats of Wistar strain were treated with high dose of insulin (4 U/kg, intraperitonely) for one or three days. Examinations at ultrastructural level showed apoptotic changes of ECs, allowing us to point out that changes mainly but not exclusively, occur in nuclei. Besides different stages of condensation and alterations of the chromatin, nuclear fragmentation was also observed. Higher number of ECs apoptotic nuclei in the BAT of hyperinsulinaemic rats was also confirmed by propidium iodide staining. Immunohistochemical localization of tumor necrosis factor-alpha (TNF-alpha) revealed increased expression in ECs of BAT of hyperinsulinaemic animals, indicating its possible role in insulin-induced apoptotic changes. These results suggest that BAT atrophy in hyperinsulinaemia is a result of endothelial and adipocyte apoptosis combined, rather than any of functional components alone.
T2  - European Journal of Histochemistry
T1  - Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha
IS  - 4
VL  - 55
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1324
ER  - 

@article{
author = "Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Buzadžić, Biljana J. and Korać, Bato and Korac, Aleksandra B",
year = "2011",
abstract = "The aim of the present study was to investigate whether hyperinsulinaemia, which frequently precedes insulin resistance syndrome (obesity, diabetes), induces apoptosis of endothelial cells (ECs) in brown adipose tissue (BAT) and causes BAT atrophy and also, to investigate the possible mechanisms underlying ECs death. In order to induce hyperinsulinaemia, adult male rats of Wistar strain were treated with high dose of insulin (4 U/kg, intraperitonely) for one or three days. Examinations at ultrastructural level showed apoptotic changes of ECs, allowing us to point out that changes mainly but not exclusively, occur in nuclei. Besides different stages of condensation and alterations of the chromatin, nuclear fragmentation was also observed. Higher number of ECs apoptotic nuclei in the BAT of hyperinsulinaemic rats was also confirmed by propidium iodide staining. Immunohistochemical localization of tumor necrosis factor-alpha (TNF-alpha) revealed increased expression in ECs of BAT of hyperinsulinaemic animals, indicating its possible role in insulin-induced apoptotic changes. These results suggest that BAT atrophy in hyperinsulinaemia is a result of endothelial and adipocyte apoptosis combined, rather than any of functional components alone.",
journal = "European Journal of Histochemistry",
title = "Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha",
number = "4",
volume = "55",
pages = "193",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1324"
}

Markelić, M. B., Velicković, K. D., Golić, I., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Buzadžić, B. J., Korać, B.,& Korac, A. B.. (2011). Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha. in European Journal of Histochemistry, 55(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1324

Markelić MB, Velicković KD, Golić I, Otašević V, Stančić A, Janković A, Vučetić M, Buzadžić BJ, Korać B, Korac AB. Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha. in European Journal of Histochemistry. 2011;55(4):null-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1324 .

Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Buzadžić, Biljana J., Korać, Bato, Korac, Aleksandra B, "Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha" in European Journal of Histochemistry, 55, no. 4 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1324 .

TY  - CONF
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Korać, Bato
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1314
C3  - Journal of Vascular Research
T1  - Insulin-induced microcirculation Remodelling in the rat brown adipose tissue
IS  - null
VL  - 48
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1314
ER  - 

@conference{
author = "Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Korać, Bato and Buzadžić, Biljana J. and Korac, Aleksandra B",
year = "2011",
journal = "Journal of Vascular Research",
title = "Insulin-induced microcirculation Remodelling in the rat brown adipose tissue",
number = "null",
volume = "48",
pages = "135",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1314"
}

Markelić, M. B., Velicković, K. D., Golić, I., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Korać, B., Buzadžić, B. J.,& Korac, A. B.. (2011). Insulin-induced microcirculation Remodelling in the rat brown adipose tissue. in Journal of Vascular Research, 48(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1314

Markelić MB, Velicković KD, Golić I, Otašević V, Stančić A, Janković A, Vučetić M, Korać B, Buzadžić BJ, Korac AB. Insulin-induced microcirculation Remodelling in the rat brown adipose tissue. in Journal of Vascular Research. 2011;48(null):null-135.
https://hdl.handle.net/21.15107/rcub_ibiss_1314 .

Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Korać, Bato, Buzadžić, Biljana J., Korac, Aleksandra B, "Insulin-induced microcirculation Remodelling in the rat brown adipose tissue" in Journal of Vascular Research, 48, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1314 .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Korać, Bato
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1123
AB  - Cold-exposure activates interscapular brown adipose tissue (IBAT) non-shivering thermogenesis that relies primarily on intensification of metabolic rate and uncoupling. During cold-acclimation, uncoupling in IBAT decreases superoxide (O(2) (center dot-)) production and as an adaptive response the activities of manganese and copper, zinc superoxide dismutase (Mn- and CuZn-SOD, respectively) are decreased, as well. However, molecular mechanisms governing this SODs adaptive response are still unsolved. Besides, knowing that NO reinforces IBAT uncoupling, we wondered whether nitric oxide (NO) is taking part in SODs regulation? Mn- and CuZn-SOD mRNA and protein expression, uncoupling protein 1 (UCP1), nitrotyrosine and nuclear factor-kappa B (NF-kappa B) immunolabeling, as well as total SOD (tSOD) activity in IBAT of rats subjected to cold (4 +/- A 1A degrees C) for 1, 3, 7, 12, 21 and 45 days and treated by l-arginine or N (omega)-nitro-l-arginine-methyl ester (l-NAME) were examined. Cold increased UCP1 immunopositivity and decreased tSOD activity during entire cold-acclimation and transiently, (day 3), activated NF-kappa B and increased Mn and CuZn-SOD mRNA expression and nitrotyrosine labeling, suggesting NO involvement in this signaling. However, SODs mRNA expression was decreasing from day 12 till the end of cold-acclimation. l-arginine augmented and prolonged cold-induced UCP1 and nitrotyrosine immunopositivity, NF-kappa B activation and SODs mRNA expression increase, while l-NAME expressed an opposite effect. Related to cold, l-arginine decreased, while l-NAME increased Mn-SOD protein expression. In contrast, neither low temperature nor both treatments applied affected CuZn-SOD protein expression. The results showed that adaptive decrease in SODs activity on uncoupling-decreased O(2) (center dot-) production was achieved already at the level of gene transcription and that NO takes part in the regulation of IBAT SOD isoforms.
PB  - Springer Nature
T2  - Genes and Nutrition
T1  - Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue
IS  - 3
VL  - 5
DO  - 10.1007/s12263-009-0162-1
SP  - 225
EP  - 235
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1123
ER  - 

@article{
author = "Otašević, Vesna and Buzadžić, Biljana J. and Korac, Aleksandra B and Korać, Bato",
year = "2010",
abstract = "Cold-exposure activates interscapular brown adipose tissue (IBAT) non-shivering thermogenesis that relies primarily on intensification of metabolic rate and uncoupling. During cold-acclimation, uncoupling in IBAT decreases superoxide (O(2) (center dot-)) production and as an adaptive response the activities of manganese and copper, zinc superoxide dismutase (Mn- and CuZn-SOD, respectively) are decreased, as well. However, molecular mechanisms governing this SODs adaptive response are still unsolved. Besides, knowing that NO reinforces IBAT uncoupling, we wondered whether nitric oxide (NO) is taking part in SODs regulation? Mn- and CuZn-SOD mRNA and protein expression, uncoupling protein 1 (UCP1), nitrotyrosine and nuclear factor-kappa B (NF-kappa B) immunolabeling, as well as total SOD (tSOD) activity in IBAT of rats subjected to cold (4 +/- A 1A degrees C) for 1, 3, 7, 12, 21 and 45 days and treated by l-arginine or N (omega)-nitro-l-arginine-methyl ester (l-NAME) were examined. Cold increased UCP1 immunopositivity and decreased tSOD activity during entire cold-acclimation and transiently, (day 3), activated NF-kappa B and increased Mn and CuZn-SOD mRNA expression and nitrotyrosine labeling, suggesting NO involvement in this signaling. However, SODs mRNA expression was decreasing from day 12 till the end of cold-acclimation. l-arginine augmented and prolonged cold-induced UCP1 and nitrotyrosine immunopositivity, NF-kappa B activation and SODs mRNA expression increase, while l-NAME expressed an opposite effect. Related to cold, l-arginine decreased, while l-NAME increased Mn-SOD protein expression. In contrast, neither low temperature nor both treatments applied affected CuZn-SOD protein expression. The results showed that adaptive decrease in SODs activity on uncoupling-decreased O(2) (center dot-) production was achieved already at the level of gene transcription and that NO takes part in the regulation of IBAT SOD isoforms.",
publisher = "Springer Nature",
journal = "Genes and Nutrition",
title = "Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue",
number = "3",
volume = "5",
doi = "10.1007/s12263-009-0162-1",
pages = "225-235",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1123"
}

Otašević, V., Buzadžić, B. J., Korac, A. B.,& Korać, B.. (2010). Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue. in Genes and Nutrition
Springer Nature., 5(3), 225-235.
https://doi.org/10.1007/s12263-009-0162-1
https://hdl.handle.net/21.15107/rcub_ibiss_1123

Otašević V, Buzadžić BJ, Korac AB, Korać B. Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue. in Genes and Nutrition. 2010;5(3):225-235.
doi:10.1007/s12263-009-0162-1
https://hdl.handle.net/21.15107/rcub_ibiss_1123 .

Otašević, Vesna, Buzadžić, Biljana J., Korac, Aleksandra B, Korać, Bato, "Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue" in Genes and Nutrition, 5, no. 3 (2010):225-235,
https://doi.org/10.1007/s12263-009-0162-1 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1123 .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Vasilijević, Ana
AU  - Janković, Aleksandra
AU  - Korać, Bato
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1356
AB  - Molecular mechanisms underlying interscapular brown adipose tissue (IBAT) thermogenesis were elucidated. Namely, gene and/or protein expression of uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor gamma (PPAR gamma), PPAR gamma-coactivator-1 alpha (PGC-1 alpha), vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) - key molecules that regulate thermogenesis-related processes - mitochondriogenesis, angiogenesis and IBAT hyperplasia, in rats subjected to cold (4 +/- 1 degrees C) for 1, 3, 7, 12, 21 and 45 days were investigated. Particularly, to examine influence of nitric oxide (NO) on IBAT thermogenic-program, cold-exposed animals were treated by L-arginine or N(omega)-nitro-L-arginine-methyl ester (L-NAME). Related to control (22 +/- 1 degrees C), cold induced time-coordinated UCP1, PPAR gamma and PGC-1 alpha transcriptional activation accompanied by PCNA activation and increased VEGF immunolabeling that correlate with endothelial NO synthase (eNOS) transcriptional activation suggesting NO involvement in these thermogenic-factors activation. Observed molecular changes were translated into increased mitochondrialremodeling, angiogenesis, and IBAT hyperplasia. L-Arginine augmented and prolonged cold-induced increase of eNOS, inducible NOS and thermogenic-molecules expression, IBAT nerve supply, vascularity, hyperplasia and mitochondrial-remodeling, while L-NAME had an opposite effects. Results show that NO improves thermogenesis-related mitochondriogenesis, angiogenesis and tissue hyperplasia, positively affecting molecular basis of these processes, suggesting that NO is an essential regulator of IBAT thermogenic-program operating, at genes, proteins and tissue structure levels. (C) 2010 Elsevier Inc. All rights reserved.
PB  - Elsevier
T2  - Comparative Biochemistry and Physiology C-Toxicology & Pharmacology
T1  - NO modulates the molecular basis of rat interscapular brown adipose tissue thermogenesis
IS  - 2
VL  - 152
DO  - 10.1016/j.cbpc.2010.03.008
SP  - 147
EP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1356
ER  - 

@article{
author = "Otašević, Vesna and Buzadžić, Biljana J. and Korac, Aleksandra B and Vasilijević, Ana and Janković, Aleksandra and Korać, Bato",
year = "2010",
abstract = "Molecular mechanisms underlying interscapular brown adipose tissue (IBAT) thermogenesis were elucidated. Namely, gene and/or protein expression of uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor gamma (PPAR gamma), PPAR gamma-coactivator-1 alpha (PGC-1 alpha), vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) - key molecules that regulate thermogenesis-related processes - mitochondriogenesis, angiogenesis and IBAT hyperplasia, in rats subjected to cold (4 +/- 1 degrees C) for 1, 3, 7, 12, 21 and 45 days were investigated. Particularly, to examine influence of nitric oxide (NO) on IBAT thermogenic-program, cold-exposed animals were treated by L-arginine or N(omega)-nitro-L-arginine-methyl ester (L-NAME). Related to control (22 +/- 1 degrees C), cold induced time-coordinated UCP1, PPAR gamma and PGC-1 alpha transcriptional activation accompanied by PCNA activation and increased VEGF immunolabeling that correlate with endothelial NO synthase (eNOS) transcriptional activation suggesting NO involvement in these thermogenic-factors activation. Observed molecular changes were translated into increased mitochondrialremodeling, angiogenesis, and IBAT hyperplasia. L-Arginine augmented and prolonged cold-induced increase of eNOS, inducible NOS and thermogenic-molecules expression, IBAT nerve supply, vascularity, hyperplasia and mitochondrial-remodeling, while L-NAME had an opposite effects. Results show that NO improves thermogenesis-related mitochondriogenesis, angiogenesis and tissue hyperplasia, positively affecting molecular basis of these processes, suggesting that NO is an essential regulator of IBAT thermogenic-program operating, at genes, proteins and tissue structure levels. (C) 2010 Elsevier Inc. All rights reserved.",
publisher = "Elsevier",
journal = "Comparative Biochemistry and Physiology C-Toxicology & Pharmacology",
title = "NO modulates the molecular basis of rat interscapular brown adipose tissue thermogenesis",
number = "2",
volume = "152",
doi = "10.1016/j.cbpc.2010.03.008",
pages = "147-159",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1356"
}

Otašević, V., Buzadžić, B. J., Korac, A. B., Vasilijević, A., Janković, A.,& Korać, B.. (2010). NO modulates the molecular basis of rat interscapular brown adipose tissue thermogenesis. in Comparative Biochemistry and Physiology C-Toxicology & Pharmacology
Elsevier., 152(2), 147-159.
https://doi.org/10.1016/j.cbpc.2010.03.008
https://hdl.handle.net/21.15107/rcub_ibiss_1356

Otašević V, Buzadžić BJ, Korac AB, Vasilijević A, Janković A, Korać B. NO modulates the molecular basis of rat interscapular brown adipose tissue thermogenesis. in Comparative Biochemistry and Physiology C-Toxicology & Pharmacology. 2010;152(2):147-159.
doi:10.1016/j.cbpc.2010.03.008
https://hdl.handle.net/21.15107/rcub_ibiss_1356 .

Otašević, Vesna, Buzadžić, Biljana J., Korac, Aleksandra B, Vasilijević, Ana, Janković, Aleksandra, Korać, Bato, "NO modulates the molecular basis of rat interscapular brown adipose tissue thermogenesis" in Comparative Biochemistry and Physiology C-Toxicology & Pharmacology, 152, no. 2 (2010):147-159,
https://doi.org/10.1016/j.cbpc.2010.03.008 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1356 .

TY  - JOUR
AU  - Stošić-Grujičić, Stanislava
AU  - Timotijević, Gordana S
AU  - Donia, Marco
AU  - Miljković, Đorđe
AU  - Mijatović, Sanja
AU  - Libra, Massimo
AU  - Maksimović-Ivanić, Danijela
AU  - Coco, Marinella
AU  - McCubrey, James A
AU  - Al-Abed, Yousef
AU  - Korac, Aleksandra B
AU  - Nicoletti, Ferdinando
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1387
AB  - The new chemical entity GIT-27NO was created by the covalent linkage of a NO moiety to the antiinflammatory isoxazoline VGX-1027 The compound has been shown to possess powerful anticancer effects both in vitro and in vivo However, its effects on nonsolid and metastatic forms of tumors have not yet been investigated We have studied the effects of GIT-27NO on the highly invasive mouse mammary TA3Ha cell line in vitro and in vivo In contrast to the conventional exogenous NO donor sodium nitroprusside, GIT-27NO successfully enhanced intracellular NO concentration in TA3Ha cells Intracellular accumulation of NO was followed by marked decrease in TA3Ha cell viability accompanied by typical apoptotic features Interestingly, inverted membrane phosphatidylserine residues. reduced volume of nucleus, condensed chromatin, and terminal fragmentation of DNA were associated with inhibited caspase-3 activity and transcription of the genes encoding caspase-3, -8, and -9 In parallel, GIT-27NO rapidly but transiently prevented the loss of p53 through phosphorylation on Ser 20 and provided the necessary signals tor the execution of downstream processes without p53 de novo synthesis The caspase-independent apoptotic-like death process triggered by GIT-27NO could be mediated by markedly down-regulated expression of the antiapoptotic Bcl-2 molecule observed in TA3Ha cells exposed to GIT-27NO In agreement with these in vitro data, GIT-27NO efficiently suppressed the growth of the ascites form and associated-lethality of tumor induced by TA3Ha cells in mice (C) 2010 Elsevier Inc All rights reserved
T2  - Free Radical Biology and Medicine
T1  - Induction of caspase-independent apoptotic-like cell death of mouse mammary tumor TA3Ha cells in vitro and reduction of their lethality in vivo by the novel chemotherapeutic agent GIT-27NO
IS  - 8
VL  - 48
EP  - 1099
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1387
ER  - 

@article{
author = "Stošić-Grujičić, Stanislava and Timotijević, Gordana S and Donia, Marco and Miljković, Đorđe and Mijatović, Sanja and Libra, Massimo and Maksimović-Ivanić, Danijela and Coco, Marinella and McCubrey, James A and Al-Abed, Yousef and Korac, Aleksandra B and Nicoletti, Ferdinando",
year = "2010",
abstract = "The new chemical entity GIT-27NO was created by the covalent linkage of a NO moiety to the antiinflammatory isoxazoline VGX-1027 The compound has been shown to possess powerful anticancer effects both in vitro and in vivo However, its effects on nonsolid and metastatic forms of tumors have not yet been investigated We have studied the effects of GIT-27NO on the highly invasive mouse mammary TA3Ha cell line in vitro and in vivo In contrast to the conventional exogenous NO donor sodium nitroprusside, GIT-27NO successfully enhanced intracellular NO concentration in TA3Ha cells Intracellular accumulation of NO was followed by marked decrease in TA3Ha cell viability accompanied by typical apoptotic features Interestingly, inverted membrane phosphatidylserine residues. reduced volume of nucleus, condensed chromatin, and terminal fragmentation of DNA were associated with inhibited caspase-3 activity and transcription of the genes encoding caspase-3, -8, and -9 In parallel, GIT-27NO rapidly but transiently prevented the loss of p53 through phosphorylation on Ser 20 and provided the necessary signals tor the execution of downstream processes without p53 de novo synthesis The caspase-independent apoptotic-like death process triggered by GIT-27NO could be mediated by markedly down-regulated expression of the antiapoptotic Bcl-2 molecule observed in TA3Ha cells exposed to GIT-27NO In agreement with these in vitro data, GIT-27NO efficiently suppressed the growth of the ascites form and associated-lethality of tumor induced by TA3Ha cells in mice (C) 2010 Elsevier Inc All rights reserved",
journal = "Free Radical Biology and Medicine",
title = "Induction of caspase-independent apoptotic-like cell death of mouse mammary tumor TA3Ha cells in vitro and reduction of their lethality in vivo by the novel chemotherapeutic agent GIT-27NO",
number = "8",
volume = "48",
pages = "1099",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1387"
}

Stošić-Grujičić, S., Timotijević, G. S., Donia, M., Miljković, Đ., Mijatović, S., Libra, M., Maksimović-Ivanić, D., Coco, M., McCubrey, J. A., Al-Abed, Y., Korac, A. B.,& Nicoletti, F.. (2010). Induction of caspase-independent apoptotic-like cell death of mouse mammary tumor TA3Ha cells in vitro and reduction of their lethality in vivo by the novel chemotherapeutic agent GIT-27NO. in Free Radical Biology and Medicine, 48(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1387

Stošić-Grujičić S, Timotijević GS, Donia M, Miljković Đ, Mijatović S, Libra M, Maksimović-Ivanić D, Coco M, McCubrey JA, Al-Abed Y, Korac AB, Nicoletti F. Induction of caspase-independent apoptotic-like cell death of mouse mammary tumor TA3Ha cells in vitro and reduction of their lethality in vivo by the novel chemotherapeutic agent GIT-27NO. in Free Radical Biology and Medicine. 2010;48(8):null-1099.
https://hdl.handle.net/21.15107/rcub_ibiss_1387 .

Stošić-Grujičić, Stanislava, Timotijević, Gordana S, Donia, Marco, Miljković, Đorđe, Mijatović, Sanja, Libra, Massimo, Maksimović-Ivanić, Danijela, Coco, Marinella, McCubrey, James A, Al-Abed, Yousef, Korac, Aleksandra B, Nicoletti, Ferdinando, "Induction of caspase-independent apoptotic-like cell death of mouse mammary tumor TA3Ha cells in vitro and reduction of their lethality in vivo by the novel chemotherapeutic agent GIT-27NO" in Free Radical Biology and Medicine, 48, no. 8 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1387 .

TY  - JOUR
AU  - Janković, Aleksandra
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Otašević, Vesna
AU  - Vasilijević, Ana
AU  - Korać, Bato
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1130
AB  - 1. Retroperitoneal white adipose tissue (RpWAT) antioxidative defense was investigated in untreated, L-arginine-treated and N(omega)-nitro-L-arginine methyl ester (L-NAME)-treated rats kept at 4 +/- 1 degrees C (1, 3, 7, 12, 21 and 45 days) and compared to control rats at 22 +/- 1 degrees C. 2. Cold-acclimation-induced RpWAT weight decrease was accompanied by a decline in glutathione level and increased activity of manganese superoxide dismutaise (MnSOD), glutathione S-transferase (GST), catalase, glutathione peroxidase and glutathione reductase at different time-points. 3. L-arginine accelerated RpWAT weight decrease, the increase in MnSOD and GST activities and the prolonged increase of catalase, MnSOD and GST activities. L-NAME delayed cold-induced catalase activity increase and tissue weight decrease. Prolonged L-NAME-treatment had a similar effect on RpWAT as L-arginine. 4. Results suggest the involvement Of L-arginine/NO pathway in RpWAT oxidative metabolic augmentation induced by cold-acclimation. (C) 2009 Elsevier Ltd. All rights reserved.
PB  - Elsevier
T2  - Journal of Thermal Biology
T1  - Antioxidative defense organization in retroperitoneal white adipose tissue during acclimation to cold-The involvement of L-arginine/NO pathway
IS  - 7
VL  - 34
DO  - 10.1016/j.jtherbio.2009.06.007
SP  - 358
EP  - 365
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1130
ER  - 

@article{
author = "Janković, Aleksandra and Buzadžić, Biljana J. and Korac, Aleksandra B and Otašević, Vesna and Vasilijević, Ana and Korać, Bato",
year = "2009",
abstract = "1. Retroperitoneal white adipose tissue (RpWAT) antioxidative defense was investigated in untreated, L-arginine-treated and N(omega)-nitro-L-arginine methyl ester (L-NAME)-treated rats kept at 4 +/- 1 degrees C (1, 3, 7, 12, 21 and 45 days) and compared to control rats at 22 +/- 1 degrees C. 2. Cold-acclimation-induced RpWAT weight decrease was accompanied by a decline in glutathione level and increased activity of manganese superoxide dismutaise (MnSOD), glutathione S-transferase (GST), catalase, glutathione peroxidase and glutathione reductase at different time-points. 3. L-arginine accelerated RpWAT weight decrease, the increase in MnSOD and GST activities and the prolonged increase of catalase, MnSOD and GST activities. L-NAME delayed cold-induced catalase activity increase and tissue weight decrease. Prolonged L-NAME-treatment had a similar effect on RpWAT as L-arginine. 4. Results suggest the involvement Of L-arginine/NO pathway in RpWAT oxidative metabolic augmentation induced by cold-acclimation. (C) 2009 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Journal of Thermal Biology",
title = "Antioxidative defense organization in retroperitoneal white adipose tissue during acclimation to cold-The involvement of L-arginine/NO pathway",
number = "7",
volume = "34",
doi = "10.1016/j.jtherbio.2009.06.007",
pages = "358-365",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1130"
}

Janković, A., Buzadžić, B. J., Korac, A. B., Otašević, V., Vasilijević, A.,& Korać, B.. (2009). Antioxidative defense organization in retroperitoneal white adipose tissue during acclimation to cold-The involvement of L-arginine/NO pathway. in Journal of Thermal Biology
Elsevier., 34(7), 358-365.
https://doi.org/10.1016/j.jtherbio.2009.06.007
https://hdl.handle.net/21.15107/rcub_ibiss_1130

Janković A, Buzadžić BJ, Korac AB, Otašević V, Vasilijević A, Korać B. Antioxidative defense organization in retroperitoneal white adipose tissue during acclimation to cold-The involvement of L-arginine/NO pathway. in Journal of Thermal Biology. 2009;34(7):358-365.
doi:10.1016/j.jtherbio.2009.06.007
https://hdl.handle.net/21.15107/rcub_ibiss_1130 .

Janković, Aleksandra, Buzadžić, Biljana J., Korac, Aleksandra B, Otašević, Vesna, Vasilijević, Ana, Korać, Bato, "Antioxidative defense organization in retroperitoneal white adipose tissue during acclimation to cold-The involvement of L-arginine/NO pathway" in Journal of Thermal Biology, 34, no. 7 (2009):358-365,
https://doi.org/10.1016/j.jtherbio.2009.06.007 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1130 .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Vasilijević, Ana
AU  - Janković, Aleksandra
AU  - Korać, Bato
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1418
AB  - We examined whether nitric oxide (NO) in vivo could induce interscapular brown adipose tissue (IBAT) glutathione synthesis. Data show that NO induces in vivo IBAT glutathione synthesis through activation of glutamate-cysteine ligase (GCL) mRNA and protein expression. This NO effect appeared to be mediated by nuclear factor-kappa B (NF-kappa B) activation. We have also observed a complex series of in vivo cellular responses during chronic inhibition of NO synthesis, suggesting that regulatory pathways unrelated to GCL alteration underlie glutathione level increase induced by N(omega)-nitro-L-arginine methyl ester (L-NAME). In general, glutathione synthesis in IBAT seemed to be finely tuned by NO to provide glutathione for either mediating the effects of NO, or for preventing potential nitrosative stress. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - L-Arginine supplementation induces glutathione synthesis in interscapular brown adipose tissue through activation of glutamate-cysteine ligase expression: The role of nitric oxide
IS  - 2-3
VL  - 182
DO  - 10.1016/j.cbi.2009.07.010
SP  - 204
EP  - 212
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1418
ER  - 

@article{
author = "Otašević, Vesna and Buzadžić, Biljana J. and Korac, Aleksandra B and Vasilijević, Ana and Janković, Aleksandra and Korać, Bato",
year = "2009",
abstract = "We examined whether nitric oxide (NO) in vivo could induce interscapular brown adipose tissue (IBAT) glutathione synthesis. Data show that NO induces in vivo IBAT glutathione synthesis through activation of glutamate-cysteine ligase (GCL) mRNA and protein expression. This NO effect appeared to be mediated by nuclear factor-kappa B (NF-kappa B) activation. We have also observed a complex series of in vivo cellular responses during chronic inhibition of NO synthesis, suggesting that regulatory pathways unrelated to GCL alteration underlie glutathione level increase induced by N(omega)-nitro-L-arginine methyl ester (L-NAME). In general, glutathione synthesis in IBAT seemed to be finely tuned by NO to provide glutathione for either mediating the effects of NO, or for preventing potential nitrosative stress. (C) 2009 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "L-Arginine supplementation induces glutathione synthesis in interscapular brown adipose tissue through activation of glutamate-cysteine ligase expression: The role of nitric oxide",
number = "2-3",
volume = "182",
doi = "10.1016/j.cbi.2009.07.010",
pages = "204-212",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1418"
}

Otašević, V., Buzadžić, B. J., Korac, A. B., Vasilijević, A., Janković, A.,& Korać, B.. (2009). L-Arginine supplementation induces glutathione synthesis in interscapular brown adipose tissue through activation of glutamate-cysteine ligase expression: The role of nitric oxide. in Chemico-Biological Interactions
Elsevier., 182(2-3), 204-212.
https://doi.org/10.1016/j.cbi.2009.07.010
https://hdl.handle.net/21.15107/rcub_ibiss_1418

Otašević V, Buzadžić BJ, Korac AB, Vasilijević A, Janković A, Korać B. L-Arginine supplementation induces glutathione synthesis in interscapular brown adipose tissue through activation of glutamate-cysteine ligase expression: The role of nitric oxide. in Chemico-Biological Interactions. 2009;182(2-3):204-212.
doi:10.1016/j.cbi.2009.07.010
https://hdl.handle.net/21.15107/rcub_ibiss_1418 .

Otašević, Vesna, Buzadžić, Biljana J., Korac, Aleksandra B, Vasilijević, Ana, Janković, Aleksandra, Korać, Bato, "L-Arginine supplementation induces glutathione synthesis in interscapular brown adipose tissue through activation of glutamate-cysteine ligase expression: The role of nitric oxide" in Chemico-Biological Interactions, 182, no. 2-3 (2009):204-212,
https://doi.org/10.1016/j.cbi.2009.07.010 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1418 .

TY  - CONF
AU  - Korac, Aleksandra B
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Buzadžić, Biljana J.
AU  - Janković, Aleksandra
AU  - Otašević, Vesna
AU  - Vasilijević, Ana
AU  - Korać, Bato
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1132
C3  - Free Radical Research
T1  - Lipofuscin accumulation in brown adipocytes of hyperinsulinaemic rats
IS  - null
VL  - 43
SP  - 267
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1132
ER  - 

@conference{
author = "Korac, Aleksandra B and Markelić, Milica B and Velicković, Ksenija D and Buzadžić, Biljana J. and Janković, Aleksandra and Otašević, Vesna and Vasilijević, Ana and Korać, Bato",
year = "2009",
journal = "Free Radical Research",
title = "Lipofuscin accumulation in brown adipocytes of hyperinsulinaemic rats",
number = "null",
volume = "43",
pages = "267-85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1132"
}

Korac, A. B., Markelić, M. B., Velicković, K. D., Buzadžić, B. J., Janković, A., Otašević, V., Vasilijević, A.,& Korać, B.. (2009). Lipofuscin accumulation in brown adipocytes of hyperinsulinaemic rats. in Free Radical Research, 43(null), 267-85.
https://hdl.handle.net/21.15107/rcub_ibiss_1132

Korac AB, Markelić MB, Velicković KD, Buzadžić BJ, Janković A, Otašević V, Vasilijević A, Korać B. Lipofuscin accumulation in brown adipocytes of hyperinsulinaemic rats. in Free Radical Research. 2009;43(null):267-85.
https://hdl.handle.net/21.15107/rcub_ibiss_1132 .

Korac, Aleksandra B, Markelić, Milica B, Velicković, Ksenija D, Buzadžić, Biljana J., Janković, Aleksandra, Otašević, Vesna, Vasilijević, Ana, Korać, Bato, "Lipofuscin accumulation in brown adipocytes of hyperinsulinaemic rats" in Free Radical Research, 43, no. null (2009):267-85,
https://hdl.handle.net/21.15107/rcub_ibiss_1132 .

TY  - JOUR
AU  - Korac, Aleksandra B
AU  - Buzadžić, Biljana J.
AU  - Otašević, Vesna
AU  - Vasilijević, Ana
AU  - Janković, Aleksandra
AU  - Korać, Bato
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1557
AB  - The aim of the present study was to explore the effect of nitric oxide on leptin immunoexpression and innervation in interscapular brown adipose tissue (IBAT) of room-and cold-acclimated rats. Animals acclimated both to room-temperature (22 +/- 1 degrees C) and cold (4 +/- 1 degrees C) were treated with L-arginine, a substrate for nitric oxide synthases (NOSs), or N?-nitro-L-arginine methyl ester (L-NAME), an inhibitor of NOSs, for 45 days. Leptin expression and localization in brown adipocytes was studied by immunohistochemistry, and innervation stained by the Bodian method. Strong leptin immunopositivity was observed in brown adipocytes cytoplasm of all room-acclimated groups, but nuclear leptin positivity was found only in L-NAME treated rats. In cold-acclimated control and L-NAME treated rats leptin immunopositivity was absent, while L-arginine treatment reversed the cold-induced suppression of leptin expression. Comparing to control, L-arginine, and even more L-NAME, at 22 +/- 1 degrees C induced greater innervation. In conclusion, L-arginine treatment changes leptin expression pattern on cold in rat IBAT.
PB  - Gdańsk: Media Group sp z o.o.
T2  - Folia Histochemica et Cytobiologica
T1  - Leptin immunoexpression and innervation in rat interscapular brown adipose tissue of cold-acclimated rats: the effects of L-arginine and L-NAME
IS  - 1
VL  - 46
DO  - 10.2478/v10042-008-0015-6
SP  - 103
EP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1557
ER  - 

@article{
author = "Korac, Aleksandra B and Buzadžić, Biljana J. and Otašević, Vesna and Vasilijević, Ana and Janković, Aleksandra and Korać, Bato",
year = "2008",
abstract = "The aim of the present study was to explore the effect of nitric oxide on leptin immunoexpression and innervation in interscapular brown adipose tissue (IBAT) of room-and cold-acclimated rats. Animals acclimated both to room-temperature (22 +/- 1 degrees C) and cold (4 +/- 1 degrees C) were treated with L-arginine, a substrate for nitric oxide synthases (NOSs), or N?-nitro-L-arginine methyl ester (L-NAME), an inhibitor of NOSs, for 45 days. Leptin expression and localization in brown adipocytes was studied by immunohistochemistry, and innervation stained by the Bodian method. Strong leptin immunopositivity was observed in brown adipocytes cytoplasm of all room-acclimated groups, but nuclear leptin positivity was found only in L-NAME treated rats. In cold-acclimated control and L-NAME treated rats leptin immunopositivity was absent, while L-arginine treatment reversed the cold-induced suppression of leptin expression. Comparing to control, L-arginine, and even more L-NAME, at 22 +/- 1 degrees C induced greater innervation. In conclusion, L-arginine treatment changes leptin expression pattern on cold in rat IBAT.",
publisher = "Gdańsk: Media Group sp z o.o.",
journal = "Folia Histochemica et Cytobiologica",
title = "Leptin immunoexpression and innervation in rat interscapular brown adipose tissue of cold-acclimated rats: the effects of L-arginine and L-NAME",
number = "1",
volume = "46",
doi = "10.2478/v10042-008-0015-6",
pages = "103-109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1557"
}

Korac, A. B., Buzadžić, B. J., Otašević, V., Vasilijević, A., Janković, A.,& Korać, B.. (2008). Leptin immunoexpression and innervation in rat interscapular brown adipose tissue of cold-acclimated rats: the effects of L-arginine and L-NAME. in Folia Histochemica et Cytobiologica
Gdańsk: Media Group sp z o.o.., 46(1), 103-109.
https://doi.org/10.2478/v10042-008-0015-6
https://hdl.handle.net/21.15107/rcub_ibiss_1557

Korac AB, Buzadžić BJ, Otašević V, Vasilijević A, Janković A, Korać B. Leptin immunoexpression and innervation in rat interscapular brown adipose tissue of cold-acclimated rats: the effects of L-arginine and L-NAME. in Folia Histochemica et Cytobiologica. 2008;46(1):103-109.
doi:10.2478/v10042-008-0015-6
https://hdl.handle.net/21.15107/rcub_ibiss_1557 .

Korac, Aleksandra B, Buzadžić, Biljana J., Otašević, Vesna, Vasilijević, Ana, Janković, Aleksandra, Korać, Bato, "Leptin immunoexpression and innervation in rat interscapular brown adipose tissue of cold-acclimated rats: the effects of L-arginine and L-NAME" in Folia Histochemica et Cytobiologica, 46, no. 1 (2008):103-109,
https://doi.org/10.2478/v10042-008-0015-6 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1557 .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Buzadžić, Biljana J.
AU  - Vasilijević, Ana
AU  - Janković, Aleksandra
AU  - Micunović, Ksenija
AU  - Korać, Bato
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1502
AB  - As a complex, cell-specific process that includes both division and clear functional differentiation of mitochondria, mitochondriogenesis is regulated by numerous endocrine and autocrine factors. In the present ultrastructural study, in vivo effects of l-arginine-nitric oxide (NO)-producing pathway on mitochondriogenesis in interscapular brown adipose tissue (IBAT) were examined. For that purpose, adult Mill Hill hybrid hooded rats were receiving l-arginine, a substrate of NO synthases (NOSs), or N(omega)-nitro-l-arginine methyl ester (l-NAME), an inhibitor of NOSs, as drinking liquids for 45 days. All experimental groups were divided into two sub-groups - acclimated to room temperature and cold. IBAT mitochondria were analyzed by transmission electron microscopy and stereology. l-Arginine treatment acted increasing the number of mitochondrial profiles per cell profile, as well as volume fraction of mitochondria per cell volume in animals maintained at room temperature. Cold-induced enhancement of number of mitochondrial profiles per cell profile was additionally increased in l-arginine-treated rats. Ultrastructural examinations of l-arginine-treated cold-acclimated animals clearly demonstrated thermogenically active mitochondria (larger size, lamellar, more numerous and well-ordered cristae in their profiles), which however were inactive in l-arginine-receiving animals kept at room temperature (small mitochondria, tubular cristae). By contrast, l-NAME treatment of rats acclimated to room temperature induced mitochondrial alterations characterized by irregular shape, short disorganized cristae and megamitochondria formation. These results showed that NO is a necessary factor for mitochondrial biogenesis and that it acts intensifying this process, but NO alone is not a sufficient stimulus for in vivo induction of mitochondriogenesis in brown adipocytes.
PB  - John Wiley and Sons
T2  - Journal of Microscopy
T1  - Nitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: ultrastructural and morphometric-stereologic studies
IS  - 3
VL  - 232
DO  - 10.1111/j.1365-2818.2008.02132.x
SP  - 542
EP  - 548
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1502
ER  - 

@article{
author = "Otašević, Vesna and Korac, Aleksandra B and Buzadžić, Biljana J. and Vasilijević, Ana and Janković, Aleksandra and Micunović, Ksenija and Korać, Bato",
year = "2008",
abstract = "As a complex, cell-specific process that includes both division and clear functional differentiation of mitochondria, mitochondriogenesis is regulated by numerous endocrine and autocrine factors. In the present ultrastructural study, in vivo effects of l-arginine-nitric oxide (NO)-producing pathway on mitochondriogenesis in interscapular brown adipose tissue (IBAT) were examined. For that purpose, adult Mill Hill hybrid hooded rats were receiving l-arginine, a substrate of NO synthases (NOSs), or N(omega)-nitro-l-arginine methyl ester (l-NAME), an inhibitor of NOSs, as drinking liquids for 45 days. All experimental groups were divided into two sub-groups - acclimated to room temperature and cold. IBAT mitochondria were analyzed by transmission electron microscopy and stereology. l-Arginine treatment acted increasing the number of mitochondrial profiles per cell profile, as well as volume fraction of mitochondria per cell volume in animals maintained at room temperature. Cold-induced enhancement of number of mitochondrial profiles per cell profile was additionally increased in l-arginine-treated rats. Ultrastructural examinations of l-arginine-treated cold-acclimated animals clearly demonstrated thermogenically active mitochondria (larger size, lamellar, more numerous and well-ordered cristae in their profiles), which however were inactive in l-arginine-receiving animals kept at room temperature (small mitochondria, tubular cristae). By contrast, l-NAME treatment of rats acclimated to room temperature induced mitochondrial alterations characterized by irregular shape, short disorganized cristae and megamitochondria formation. These results showed that NO is a necessary factor for mitochondrial biogenesis and that it acts intensifying this process, but NO alone is not a sufficient stimulus for in vivo induction of mitochondriogenesis in brown adipocytes.",
publisher = "John Wiley and Sons",
journal = "Journal of Microscopy",
title = "Nitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: ultrastructural and morphometric-stereologic studies",
number = "3",
volume = "232",
doi = "10.1111/j.1365-2818.2008.02132.x",
pages = "542-548",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1502"
}

Otašević, V., Korac, A. B., Buzadžić, B. J., Vasilijević, A., Janković, A., Micunović, K.,& Korać, B.. (2008). Nitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: ultrastructural and morphometric-stereologic studies. in Journal of Microscopy
John Wiley and Sons., 232(3), 542-548.
https://doi.org/10.1111/j.1365-2818.2008.02132.x
https://hdl.handle.net/21.15107/rcub_ibiss_1502

Otašević V, Korac AB, Buzadžić BJ, Vasilijević A, Janković A, Micunović K, Korać B. Nitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: ultrastructural and morphometric-stereologic studies. in Journal of Microscopy. 2008;232(3):542-548.
doi:10.1111/j.1365-2818.2008.02132.x
https://hdl.handle.net/21.15107/rcub_ibiss_1502 .

Otašević, Vesna, Korac, Aleksandra B, Buzadžić, Biljana J., Vasilijević, Ana, Janković, Aleksandra, Micunović, Ksenija, Korać, Bato, "Nitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: ultrastructural and morphometric-stereologic studies" in Journal of Microscopy, 232, no. 3 (2008):542-548,
https://doi.org/10.1111/j.1365-2818.2008.02132.x .,
https://hdl.handle.net/21.15107/rcub_ibiss_1502 .