TY  - JOUR
AU  - Šaponjić, Jasna
AU  - Mejías, Rebeca
AU  - Nikolovski, Neda
AU  - Dragic, Milorad
AU  - Canak, Asuman
AU  - Papoutsopoulou, Stamatia
AU  - Gürsoy-Özdemir, Yasemin
AU  - Fladmark, Kari
AU  - Ntavaroukas, Panagiotis
AU  - Bayar Muluk, Nuray
AU  - Zeljkovic Jovanovic, Milica
AU  - Fontán-Lozano, Ángela
AU  - Comi, Cristoforo
AU  - Marino, Franca
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6685
AB  - Parkinson’s disease (PD) is a chronic, age-related, progressive multisystem disease associated with neuroinflammation and immune dysfunction. This review discusses the methodological
approaches used to study the changes in central and peripheral immunity in PD, the advantages and
limitations of the techniques, and their applicability to humans. Although a single animal model
cannot replicate all pathological features of the human disease, neuroinflammation is present in
most animal models of PD and plays a critical role in understanding the involvement of the immune
system (IS) in the pathogenesis of PD. The IS and its interactions with different cell types in the
central nervous system (CNS) play an important role in the pathogenesis of PD. Even though culture
models do not fully reflect the complexity of disease progression, they are limited in their ability to
mimic long-term effects and need validation through in vivo studies. They are an indispensable tool
for understanding the interplay between the IS and the pathogenesis of this disease. Understanding
the immune-mediated mechanisms may lead to potential therapeutic targets for the treatment of PD.
We believe that the development of methodological guidelines for experiments with animal models
and PD patients is crucial to ensure the validity and consistency of the results.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Experimental Models to Study Immune Dysfunction in the Pathogenesis of Parkinson’s Disease
IS  - 8
VL  - 25
DO  - 10.3390/ijms25084330
SP  - 4330
ER  - 

@article{
author = "Šaponjić, Jasna and Mejías, Rebeca and Nikolovski, Neda and Dragic, Milorad and Canak, Asuman and Papoutsopoulou, Stamatia and Gürsoy-Özdemir, Yasemin and Fladmark, Kari and Ntavaroukas, Panagiotis and Bayar Muluk, Nuray and Zeljkovic Jovanovic, Milica and Fontán-Lozano, Ángela and Comi, Cristoforo and Marino, Franca",
year = "2024",
abstract = "Parkinson’s disease (PD) is a chronic, age-related, progressive multisystem disease associated with neuroinflammation and immune dysfunction. This review discusses the methodological
approaches used to study the changes in central and peripheral immunity in PD, the advantages and
limitations of the techniques, and their applicability to humans. Although a single animal model
cannot replicate all pathological features of the human disease, neuroinflammation is present in
most animal models of PD and plays a critical role in understanding the involvement of the immune
system (IS) in the pathogenesis of PD. The IS and its interactions with different cell types in the
central nervous system (CNS) play an important role in the pathogenesis of PD. Even though culture
models do not fully reflect the complexity of disease progression, they are limited in their ability to
mimic long-term effects and need validation through in vivo studies. They are an indispensable tool
for understanding the interplay between the IS and the pathogenesis of this disease. Understanding
the immune-mediated mechanisms may lead to potential therapeutic targets for the treatment of PD.
We believe that the development of methodological guidelines for experiments with animal models
and PD patients is crucial to ensure the validity and consistency of the results.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Experimental Models to Study Immune Dysfunction in the Pathogenesis of Parkinson’s Disease",
number = "8",
volume = "25",
doi = "10.3390/ijms25084330",
pages = "4330"
}

Šaponjić, J., Mejías, R., Nikolovski, N., Dragic, M., Canak, A., Papoutsopoulou, S., Gürsoy-Özdemir, Y., Fladmark, K., Ntavaroukas, P., Bayar Muluk, N., Zeljkovic Jovanovic, M., Fontán-Lozano, Á., Comi, C.,& Marino, F.. (2024). Experimental Models to Study Immune Dysfunction in the Pathogenesis of Parkinson’s Disease. in International Journal of Molecular Sciences
Basel: MDPI., 25(8), 4330.
https://doi.org/10.3390/ijms25084330

Šaponjić J, Mejías R, Nikolovski N, Dragic M, Canak A, Papoutsopoulou S, Gürsoy-Özdemir Y, Fladmark K, Ntavaroukas P, Bayar Muluk N, Zeljkovic Jovanovic M, Fontán-Lozano Á, Comi C, Marino F. Experimental Models to Study Immune Dysfunction in the Pathogenesis of Parkinson’s Disease. in International Journal of Molecular Sciences. 2024;25(8):4330.
doi:10.3390/ijms25084330 .

Šaponjić, Jasna, Mejías, Rebeca, Nikolovski, Neda, Dragic, Milorad, Canak, Asuman, Papoutsopoulou, Stamatia, Gürsoy-Özdemir, Yasemin, Fladmark, Kari, Ntavaroukas, Panagiotis, Bayar Muluk, Nuray, Zeljkovic Jovanovic, Milica, Fontán-Lozano, Ángela, Comi, Cristoforo, Marino, Franca, "Experimental Models to Study Immune Dysfunction in the Pathogenesis of Parkinson’s Disease" in International Journal of Molecular Sciences, 25, no. 8 (2024):4330,
https://doi.org/10.3390/ijms25084330 . .

TY  - CONF
AU  - Novaković, Andrea
AU  - Radovanović, Ljiljana
AU  - Petrović, Jelena
AU  - Šaponjić, Jasna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5841
AB  - We examined the effects of ketamine/diazepam and propofol anesthesia on
hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV)-expressing
interneurons in the rat.
A total of 20 adult male Wistar rats were divided into two experimental groups - half
were anesthetized with ketamine/diazepam (100 mg/kg, Zoletil® 50), and the other
half received propofol anesthesia (100 mg/kg; Propofol Lipuro 2% (20mg/ml). We
performed immunohistochemistry protocols for PV and postsynaptic density protein
95 (PSD-95) staining on free-floating 40-µm brain slices. We used PSD-95 as an
excitatory synaptic marker to test local excitation changes along with changes in PV
expression.
Our results show significant suppression of GABAergic PV-expressing interneurons
during ketamine/diazepam anesthesia compared with propofol anesthesia, in the
dentate gyrus and CA3 regions of the hippocampus (z ≥ -4.16, p ≤ 10-3), and in the
RT. Moreover, this suppression resulted in an increase in PSD-95 expression only in
the hippocampus of rats anesthetized with ketamine/diazepam. Topographically
distinct effects of propofol anesthesia were not detected.
The observed imbalance between excitation and inhibition at the level of the
hippocampus during ketamine/diazepam anesthesia could be a consequence of lower
interneuronal GABA activity. Conversely, the topographically uniform expression of
PSD-95 during propofol anesthesia together with higher expression of GABAergic
interneurons could possibly indicate a stronger effect of GABA-mediated inhibition in
the hippocampus compared with ketamine/diazepam anesthesia.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons
IS  - 52
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5841
ER  - 

@conference{
author = "Novaković, Andrea and Radovanović, Ljiljana and Petrović, Jelena and Šaponjić, Jasna",
year = "2023",
abstract = "We examined the effects of ketamine/diazepam and propofol anesthesia on
hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV)-expressing
interneurons in the rat.
A total of 20 adult male Wistar rats were divided into two experimental groups - half
were anesthetized with ketamine/diazepam (100 mg/kg, Zoletil® 50), and the other
half received propofol anesthesia (100 mg/kg; Propofol Lipuro 2% (20mg/ml). We
performed immunohistochemistry protocols for PV and postsynaptic density protein
95 (PSD-95) staining on free-floating 40-µm brain slices. We used PSD-95 as an
excitatory synaptic marker to test local excitation changes along with changes in PV
expression.
Our results show significant suppression of GABAergic PV-expressing interneurons
during ketamine/diazepam anesthesia compared with propofol anesthesia, in the
dentate gyrus and CA3 regions of the hippocampus (z ≥ -4.16, p ≤ 10-3), and in the
RT. Moreover, this suppression resulted in an increase in PSD-95 expression only in
the hippocampus of rats anesthetized with ketamine/diazepam. Topographically
distinct effects of propofol anesthesia were not detected.
The observed imbalance between excitation and inhibition at the level of the
hippocampus during ketamine/diazepam anesthesia could be a consequence of lower
interneuronal GABA activity. Conversely, the topographically uniform expression of
PSD-95 during propofol anesthesia together with higher expression of GABAergic
interneurons could possibly indicate a stronger effect of GABA-mediated inhibition in
the hippocampus compared with ketamine/diazepam anesthesia.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons",
number = "52",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5841"
}

Novaković, A., Radovanović, L., Petrović, J.,& Šaponjić, J.. (2023). Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society.(52).
https://hdl.handle.net/21.15107/rcub_ibiss_5841

Novaković A, Radovanović L, Petrović J, Šaponjić J. Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;(52).
https://hdl.handle.net/21.15107/rcub_ibiss_5841 .

Novaković, Andrea, Radovanović, Ljiljana, Petrović, Jelena, Šaponjić, Jasna, "Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia, no. 52 (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5841 .

TY  - CONF
AU  - Šaponjić, Jasna
AU  - Radovanović, Ljiljana
AU  - Novaković, Andrea
AU  - Petrović, Jelena
PY  - 2023
UR  - https://esleepeurope.eu/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6243
AB  - Background: The GABAergic mechanism is an important target for the action of anesthetics and the promotion of sleep. We investigated the changes in
hippocampal and reticulo-thalamic nucleus (RT) GABAergic parvalbumin (PV)-expressing interneurons as possible underlying mechanisms of the different
local cortical and hippocampal EEG microstructures during NREM sleep compared with anesthesia-induced unconsciousness by two anesthetics with
different main mechanisms of action.
Methods: Twenty adult male Wistar rats were implanted for sleep recordings. After 3 hours of sleep recording, half of the rats were anesthetized with
ketamine/diazepam (100 mg/kg, i.p.) and the other half with propofol (100 mg/kg, i.p.). We recorded EEGs of the motor cortex and hippocampus during the
one-hour stable surgical phase of both anesthetics. The EEG microstructures of the motor cortex and hippocampus in local NREM sleep were compared with
their EEG microstructures during 30 minutes of unconsciousness induced by a given anesthetic. At the end of each recording under stable anesthesia, rats
were sacrificed for further PV and postsynaptic density protein 95 (PSD-95) immunohistochemistry.
Results: All three states of unconsciousness differed in motor cortical and hippocampal EEG microstructures (χ2≥9.46;p≤0.01). During propofol-induced
unconsciousness, attenuated delta and augmented sigma/beta amplitudes (z≥-4.13;p≤0.01) were the globally expressed difference, whereas increased
gamma amplitude (z=2.35;p=0.02) was the only difference at the motor-cortical level compared to NREM sleep. During ketamine/diazepam-induced
unconsciousness, attenuated theta (z≥-5.53;p≤10-4) and increased beta/gamma amplitudes (z≥-4.82;p≤10-4) were the globally expressed difference from
NREM sleep. Both anesthesia-induced unconsciousness expressed globally as increased beta amplitude (z≥-4.13;p≤10-3) and increased motor-cortical
gamma amplitude (z≥-4.20;p≤0.02) compared to NREM sleep. In contrast to propofol anesthesia, there was significant suppression of PV expression in the
hippocampus (z≤-2.71;p≤ .01) and RT during ketamine/diazepam anesthesia in all rats, but only in the hippocampus was there an inhibitory/excitatory
imbalance (increased PSD-95 expression).
Conclusions: Although anesthesia and sleep share many neurobiological features, they are distinct states in terms of local EEG microstructure and
underlying GABAergic and molecular substrate in local neuronal networks.
PB  - European Sleep Research Society
C3  - eSLEEP EUROPE; 2023 Oct 4-6; Online
T1  - GABAergic parvalbumin-expressing interneurons underlie distinct local EEG microstructure during different states of unconsciousness in rats
SP  - 2
EP  - 2
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6243
ER  - 

@conference{
author = "Šaponjić, Jasna and Radovanović, Ljiljana and Novaković, Andrea and Petrović, Jelena",
year = "2023",
abstract = "Background: The GABAergic mechanism is an important target for the action of anesthetics and the promotion of sleep. We investigated the changes in
hippocampal and reticulo-thalamic nucleus (RT) GABAergic parvalbumin (PV)-expressing interneurons as possible underlying mechanisms of the different
local cortical and hippocampal EEG microstructures during NREM sleep compared with anesthesia-induced unconsciousness by two anesthetics with
different main mechanisms of action.
Methods: Twenty adult male Wistar rats were implanted for sleep recordings. After 3 hours of sleep recording, half of the rats were anesthetized with
ketamine/diazepam (100 mg/kg, i.p.) and the other half with propofol (100 mg/kg, i.p.). We recorded EEGs of the motor cortex and hippocampus during the
one-hour stable surgical phase of both anesthetics. The EEG microstructures of the motor cortex and hippocampus in local NREM sleep were compared with
their EEG microstructures during 30 minutes of unconsciousness induced by a given anesthetic. At the end of each recording under stable anesthesia, rats
were sacrificed for further PV and postsynaptic density protein 95 (PSD-95) immunohistochemistry.
Results: All three states of unconsciousness differed in motor cortical and hippocampal EEG microstructures (χ2≥9.46;p≤0.01). During propofol-induced
unconsciousness, attenuated delta and augmented sigma/beta amplitudes (z≥-4.13;p≤0.01) were the globally expressed difference, whereas increased
gamma amplitude (z=2.35;p=0.02) was the only difference at the motor-cortical level compared to NREM sleep. During ketamine/diazepam-induced
unconsciousness, attenuated theta (z≥-5.53;p≤10-4) and increased beta/gamma amplitudes (z≥-4.82;p≤10-4) were the globally expressed difference from
NREM sleep. Both anesthesia-induced unconsciousness expressed globally as increased beta amplitude (z≥-4.13;p≤10-3) and increased motor-cortical
gamma amplitude (z≥-4.20;p≤0.02) compared to NREM sleep. In contrast to propofol anesthesia, there was significant suppression of PV expression in the
hippocampus (z≤-2.71;p≤ .01) and RT during ketamine/diazepam anesthesia in all rats, but only in the hippocampus was there an inhibitory/excitatory
imbalance (increased PSD-95 expression).
Conclusions: Although anesthesia and sleep share many neurobiological features, they are distinct states in terms of local EEG microstructure and
underlying GABAergic and molecular substrate in local neuronal networks.",
publisher = "European Sleep Research Society",
journal = "eSLEEP EUROPE; 2023 Oct 4-6; Online",
title = "GABAergic parvalbumin-expressing interneurons underlie distinct local EEG microstructure during different states of unconsciousness in rats",
pages = "2-2",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6243"
}

Šaponjić, J., Radovanović, L., Novaković, A.,& Petrović, J.. (2023). GABAergic parvalbumin-expressing interneurons underlie distinct local EEG microstructure during different states of unconsciousness in rats. in eSLEEP EUROPE; 2023 Oct 4-6; Online
European Sleep Research Society., 2-2.
https://hdl.handle.net/21.15107/rcub_ibiss_6243

Šaponjić J, Radovanović L, Novaković A, Petrović J. GABAergic parvalbumin-expressing interneurons underlie distinct local EEG microstructure during different states of unconsciousness in rats. in eSLEEP EUROPE; 2023 Oct 4-6; Online. 2023;:2-2.
https://hdl.handle.net/21.15107/rcub_ibiss_6243 .

Šaponjić, Jasna, Radovanović, Ljiljana, Novaković, Andrea, Petrović, Jelena, "GABAergic parvalbumin-expressing interneurons underlie distinct local EEG microstructure during different states of unconsciousness in rats" in eSLEEP EUROPE; 2023 Oct 4-6; Online (2023):2-2,
https://hdl.handle.net/21.15107/rcub_ibiss_6243 .

TY  - CONF
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
AU  - Petrović, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5828
AB  - We investigated the role of hippocampal GABAergic parvalbumin-expressing (PV)
interneurons in spatial and hippocampus-dependent memory abilities in rat models of
Parkinson's disease (PD).
Experiments were performed in adult male Wistar rats, including physiological
controls (n=14) and toxin lesion-induced PD models: PD cholinopathy (n=10),
hemiparkinsonism (n=7), and hemiparkinsonism with PD cholinopathy (n=6).
Behavioral assessments and PV immunohistochemistry were performed 14 and 42
days after lesions. Spatial habituation test and novel object recognition test were used
to assess spatial and hippocampus-dependent short- and long-term recognition
memory.
All experimental groups had no motor impairments during the follow-up period
(X2
≥2.01, p≥0.07). Although hippocampal PV expression remained unchanged over
time in PD cholinopathy (z≥-1.91, p≥0.06), we evidenced impairments in spatial,
short- and long-term recognition memory, but only at day 42 (X2
≥0.38, p=0.83;
t=0.13, p=0.91). In the hemiparkinsonian rats, unchanged hippocampal PV expression
(z≥-1.52, p≥0.14) was followed by impairment in spatial memory (X2
≥2.87, p≥0.22),
but both recognition memories were intact over time (t≥3.16, p≤0.03). In the
hemiparkinsonian rats with PD cholinopathy, long-lasting impairment of spatial
memory (X2
≥0.72, p≥0.22) was followed by delayed short- and long-term impairment
of recognition memory (t=-0.24, p=0.82) along with hippocampal PV suppression
(z=-3.17, p=10-3), which was functionally coupled to impairment of recognition
memory (r=0.52, p=0.04).
Our results suggest that dopaminergic denervation plays an important role in
impairing spatial memory in the prodromal stage of PD, whereas cholinergic
denervation and hippocampal PV suppression impair short- and long-term memory in
a delayed manner in PD cholinopathy and hemiparkinsonism with PD cholinopathy.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - GABAergic parvalbumin-expressing interneurons play a role in memory impairment in rat models of Parkinson's disease
IS  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5828
ER  - 

@conference{
author = "Radovanović, Ljiljana and Šaponjić, Jasna and Petrović, Jelena",
year = "2023",
abstract = "We investigated the role of hippocampal GABAergic parvalbumin-expressing (PV)
interneurons in spatial and hippocampus-dependent memory abilities in rat models of
Parkinson's disease (PD).
Experiments were performed in adult male Wistar rats, including physiological
controls (n=14) and toxin lesion-induced PD models: PD cholinopathy (n=10),
hemiparkinsonism (n=7), and hemiparkinsonism with PD cholinopathy (n=6).
Behavioral assessments and PV immunohistochemistry were performed 14 and 42
days after lesions. Spatial habituation test and novel object recognition test were used
to assess spatial and hippocampus-dependent short- and long-term recognition
memory.
All experimental groups had no motor impairments during the follow-up period
(X2
≥2.01, p≥0.07). Although hippocampal PV expression remained unchanged over
time in PD cholinopathy (z≥-1.91, p≥0.06), we evidenced impairments in spatial,
short- and long-term recognition memory, but only at day 42 (X2
≥0.38, p=0.83;
t=0.13, p=0.91). In the hemiparkinsonian rats, unchanged hippocampal PV expression
(z≥-1.52, p≥0.14) was followed by impairment in spatial memory (X2
≥2.87, p≥0.22),
but both recognition memories were intact over time (t≥3.16, p≤0.03). In the
hemiparkinsonian rats with PD cholinopathy, long-lasting impairment of spatial
memory (X2
≥0.72, p≥0.22) was followed by delayed short- and long-term impairment
of recognition memory (t=-0.24, p=0.82) along with hippocampal PV suppression
(z=-3.17, p=10-3), which was functionally coupled to impairment of recognition
memory (r=0.52, p=0.04).
Our results suggest that dopaminergic denervation plays an important role in
impairing spatial memory in the prodromal stage of PD, whereas cholinergic
denervation and hippocampal PV suppression impair short- and long-term memory in
a delayed manner in PD cholinopathy and hemiparkinsonism with PD cholinopathy.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "GABAergic parvalbumin-expressing interneurons play a role in memory impairment in rat models of Parkinson's disease",
number = "49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5828"
}

Radovanović, L., Šaponjić, J.,& Petrović, J.. (2023). GABAergic parvalbumin-expressing interneurons play a role in memory impairment in rat models of Parkinson's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society.(49).
https://hdl.handle.net/21.15107/rcub_ibiss_5828

Radovanović L, Šaponjić J, Petrović J. GABAergic parvalbumin-expressing interneurons play a role in memory impairment in rat models of Parkinson's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;(49).
https://hdl.handle.net/21.15107/rcub_ibiss_5828 .

Radovanović, Ljiljana, Šaponjić, Jasna, Petrović, Jelena, "GABAergic parvalbumin-expressing interneurons play a role in memory impairment in rat models of Parkinson's disease" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia, no. 49 (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5828 .

TY  - CONF
AU  - Novaković, Andrea
AU  - Radovanović, Ljiljana
AU  - Petrović, Jelena
AU  - Šaponjić, Jasna
PY  - 2023
UR  - https://fensrm2023algarve.pt/scientific-programme/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5810
AB  - We examined the effects of ketamine/diazepam and propofol anesthesia on hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV)-expressing interneurons in the rat.
A total of 20 adult male Wistar rats were divided into two experimental groups - half were anesthetized with ketamine/diazepam (100 mg/kg, Zoletil® 50), and the other half received propofol anesthesia (100 mg/kg; Propofol Lipuro 2% (20mg/ml). We performed immunohistochemistry protocols for PV and postsynaptic density protein 95 (PSD-95) staining on free-floating 40-µm brain slices. We used PSD-95 as an excitatory synaptic marker to test local excitation changes along with changes in PV expression.
Our results show significant suppression of GABAergic PV-expressing interneurons during ketamine/diazepam anesthesia compared with propofol anesthesia, in the dentate gyrus and CA3 regions of the hippocampus, and in the RT. Moreover, this suppression resulted in an increase in PSD-95 expression only in the hippocampus of rats anesthetized with ketamine/diazepam. Topographically distinct effects of propofol anesthesia were not detected.
The observed imbalance between excitation and inhibition at the level of the hippocampus during ketamine/diazepam anesthesia could be a consequence of lower interneuronal GABA activity. Conversely, the topographically uniform expression of PSD-95 during propofol anesthesia together with higher expression of GABAergic interneurons could possibly indicate a stronger effect of GABA-mediated inhibition in the hippocampus compared with ketamine/diazepam anesthesia.
PB  - Federation of European Neuroscience Societies
C3  - FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal
T1  - Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5810
ER  - 

@conference{
author = "Novaković, Andrea and Radovanović, Ljiljana and Petrović, Jelena and Šaponjić, Jasna",
year = "2023",
abstract = "We examined the effects of ketamine/diazepam and propofol anesthesia on hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV)-expressing interneurons in the rat.
A total of 20 adult male Wistar rats were divided into two experimental groups - half were anesthetized with ketamine/diazepam (100 mg/kg, Zoletil® 50), and the other half received propofol anesthesia (100 mg/kg; Propofol Lipuro 2% (20mg/ml). We performed immunohistochemistry protocols for PV and postsynaptic density protein 95 (PSD-95) staining on free-floating 40-µm brain slices. We used PSD-95 as an excitatory synaptic marker to test local excitation changes along with changes in PV expression.
Our results show significant suppression of GABAergic PV-expressing interneurons during ketamine/diazepam anesthesia compared with propofol anesthesia, in the dentate gyrus and CA3 regions of the hippocampus, and in the RT. Moreover, this suppression resulted in an increase in PSD-95 expression only in the hippocampus of rats anesthetized with ketamine/diazepam. Topographically distinct effects of propofol anesthesia were not detected.
The observed imbalance between excitation and inhibition at the level of the hippocampus during ketamine/diazepam anesthesia could be a consequence of lower interneuronal GABA activity. Conversely, the topographically uniform expression of PSD-95 during propofol anesthesia together with higher expression of GABAergic interneurons could possibly indicate a stronger effect of GABA-mediated inhibition in the hippocampus compared with ketamine/diazepam anesthesia.",
publisher = "Federation of European Neuroscience Societies",
journal = "FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal",
title = "Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5810"
}

Novaković, A., Radovanović, L., Petrović, J.,& Šaponjić, J.. (2023). Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat. in FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal
Federation of European Neuroscience Societies..
https://hdl.handle.net/21.15107/rcub_ibiss_5810

Novaković A, Radovanović L, Petrović J, Šaponjić J. Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat. in FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5810 .

Novaković, Andrea, Radovanović, Ljiljana, Petrović, Jelena, Šaponjić, Jasna, "Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat" in FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5810 .

TY  - JOUR
AU  - Radovanović, Ljiljana
AU  - Novaković, Andrea
AU  - Petrović, Jelena
AU  - Šaponjić, Jasna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5547
AB  - We traced the changes in GABAergic parvalbumin (PV)-expressing interneurons of the hippocampus and reticulo-thalamic nucleus (RT) as possible underlying mechanisms of the different local cortical and hippocampal electroencephalographic (EEG) microstructures during the non-rapid-eye movement (NREM) sleep compared with anesthesia-induced unconsciousness by two anesthetics with different main mechanisms of action (ketamine/diazepam versus propofol). After 3 h of recording their sleep, the rats were divided into two experimental groups: one half received ketamine/diazepam anesthesia and the other half received propofol anesthesia. We simultaneously recorded the EEG of the motor cortex and hippocampus during sleep and during 1 h of surgical anesthesia. We performed immunohistochemistry and analyzed the PV and postsynaptic density protein 95 (PSD-95) expression. PV suppression in the hippocampus and at RT underlies the global theta amplitude attenuation and hippocampal gamma augmentation that is a unique feature of ketamine-induced versus propofol-induced unconsciousness and NREM sleep. While PV suppression resulted in an increase in hippocampal PSD-95 expression, there was no imbalance between inhibition and excitation during ketamine/diazepam anesthesia compared with propofol anesthesia in RT. This increased excitation could be a consequence of a lower GABA interneuronal activity and an additional mechanism underlying the unique local EEG microstructure in the hippocampus during ketamine/diazepam anesthesia.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Different Alterations of Hippocampal and Reticulo-Thalamic GABAergic Parvalbumin-Expressing Interneurons Underlie Different States of Unconsciousness
IS  - 7
VL  - 24
DO  - 10.3390/ijms24076769
SP  - 6769
ER  - 

@article{
author = "Radovanović, Ljiljana and Novaković, Andrea and Petrović, Jelena and Šaponjić, Jasna",
year = "2023",
abstract = "We traced the changes in GABAergic parvalbumin (PV)-expressing interneurons of the hippocampus and reticulo-thalamic nucleus (RT) as possible underlying mechanisms of the different local cortical and hippocampal electroencephalographic (EEG) microstructures during the non-rapid-eye movement (NREM) sleep compared with anesthesia-induced unconsciousness by two anesthetics with different main mechanisms of action (ketamine/diazepam versus propofol). After 3 h of recording their sleep, the rats were divided into two experimental groups: one half received ketamine/diazepam anesthesia and the other half received propofol anesthesia. We simultaneously recorded the EEG of the motor cortex and hippocampus during sleep and during 1 h of surgical anesthesia. We performed immunohistochemistry and analyzed the PV and postsynaptic density protein 95 (PSD-95) expression. PV suppression in the hippocampus and at RT underlies the global theta amplitude attenuation and hippocampal gamma augmentation that is a unique feature of ketamine-induced versus propofol-induced unconsciousness and NREM sleep. While PV suppression resulted in an increase in hippocampal PSD-95 expression, there was no imbalance between inhibition and excitation during ketamine/diazepam anesthesia compared with propofol anesthesia in RT. This increased excitation could be a consequence of a lower GABA interneuronal activity and an additional mechanism underlying the unique local EEG microstructure in the hippocampus during ketamine/diazepam anesthesia.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Different Alterations of Hippocampal and Reticulo-Thalamic GABAergic Parvalbumin-Expressing Interneurons Underlie Different States of Unconsciousness",
number = "7",
volume = "24",
doi = "10.3390/ijms24076769",
pages = "6769"
}

Radovanović, L., Novaković, A., Petrović, J.,& Šaponjić, J.. (2023). Different Alterations of Hippocampal and Reticulo-Thalamic GABAergic Parvalbumin-Expressing Interneurons Underlie Different States of Unconsciousness. in International Journal of Molecular Sciences
Basel: MDPI., 24(7), 6769.
https://doi.org/10.3390/ijms24076769

Radovanović L, Novaković A, Petrović J, Šaponjić J. Different Alterations of Hippocampal and Reticulo-Thalamic GABAergic Parvalbumin-Expressing Interneurons Underlie Different States of Unconsciousness. in International Journal of Molecular Sciences. 2023;24(7):6769.
doi:10.3390/ijms24076769 .

Radovanović, Ljiljana, Novaković, Andrea, Petrović, Jelena, Šaponjić, Jasna, "Different Alterations of Hippocampal and Reticulo-Thalamic GABAergic Parvalbumin-Expressing Interneurons Underlie Different States of Unconsciousness" in International Journal of Molecular Sciences, 24, no. 7 (2023):6769,
https://doi.org/10.3390/ijms24076769 . .

TY  - CONF
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
AU  - Petrović, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5556
AB  - We investigated the alterations of reticulo-thalamic (RT) GABAergic parvalbumin (PV+) interneurons and synaptic reorganization underlying the altered hippocampal high voltage sleep spindle (HVS) dynamics during REM sleep in the rat
models of Parkinson’s disease (PD). Adult male Wistar rats were implanted for 6h sleep recording during light phase in four
experimental groups: control (implanted controls), PD cholinopathy (bilateral lesion of the nucleus pedunculopontinus
tegmentalis−PPT), hemiparkinsonism (unilateral lesion of the nucleus substantiae nigrae pars compacta−SNpc) and
hemiparkinsonism with PD cholinopathy (unilateral SNpc/bilateral PPT lesion). Following 14 days of the surgical procedure
we differentiated the Wake/NREM/REM 10s epochs, and the HVSs detection and extraction was done automatically (4.1–10
Hz band pass filter, 1s minimum duration) and visually validated. Hippocampal HVS dynamics were analyzed during 1h of
NREM/REM sleep. Alterations of the PV+ interneurons and synaptic re-organization within the RT were determined by the
parvalbumin, MAP2 and PSD-95 immunostaining. REM sleep is a predisposing state for the HVSs induction in all
experimental models of PD neuropathology. Whereas the PD cholinopathy induced the prolongation and higher density of
hippocampal HVSs, the hemiparkinsonism with PD cholinopathy increased the hippocampal HVSs intrinsic frequency during
REM sleep. In contrast to the unaltered PV+ interneurons/partially enhanced MAP2/suppressed PSD-95 expression during
PD cholinopathy, we evidenced the PV+ interneurons reduction/enhanced MAP2/no change of PSD-95 expression in the RT
during hemiparkinsonism with PD cholinopathy. Distinct PV+ interneurons alteration and inhibition/excitation balance in the
RT could be the underlying mechanisms of HVS generation/alteration during REM sleep in the parkinsonian rats.
PB  - Federation of European Neuroscience Societies
C3  - E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France
T1  - Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats
SP  - 2231
EP  - 2232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5556
ER  - 

@conference{
author = "Radovanović, Ljiljana and Šaponjić, Jasna and Petrović, Jelena",
year = "2022",
abstract = "We investigated the alterations of reticulo-thalamic (RT) GABAergic parvalbumin (PV+) interneurons and synaptic reorganization underlying the altered hippocampal high voltage sleep spindle (HVS) dynamics during REM sleep in the rat
models of Parkinson’s disease (PD). Adult male Wistar rats were implanted for 6h sleep recording during light phase in four
experimental groups: control (implanted controls), PD cholinopathy (bilateral lesion of the nucleus pedunculopontinus
tegmentalis−PPT), hemiparkinsonism (unilateral lesion of the nucleus substantiae nigrae pars compacta−SNpc) and
hemiparkinsonism with PD cholinopathy (unilateral SNpc/bilateral PPT lesion). Following 14 days of the surgical procedure
we differentiated the Wake/NREM/REM 10s epochs, and the HVSs detection and extraction was done automatically (4.1–10
Hz band pass filter, 1s minimum duration) and visually validated. Hippocampal HVS dynamics were analyzed during 1h of
NREM/REM sleep. Alterations of the PV+ interneurons and synaptic re-organization within the RT were determined by the
parvalbumin, MAP2 and PSD-95 immunostaining. REM sleep is a predisposing state for the HVSs induction in all
experimental models of PD neuropathology. Whereas the PD cholinopathy induced the prolongation and higher density of
hippocampal HVSs, the hemiparkinsonism with PD cholinopathy increased the hippocampal HVSs intrinsic frequency during
REM sleep. In contrast to the unaltered PV+ interneurons/partially enhanced MAP2/suppressed PSD-95 expression during
PD cholinopathy, we evidenced the PV+ interneurons reduction/enhanced MAP2/no change of PSD-95 expression in the RT
during hemiparkinsonism with PD cholinopathy. Distinct PV+ interneurons alteration and inhibition/excitation balance in the
RT could be the underlying mechanisms of HVS generation/alteration during REM sleep in the parkinsonian rats.",
publisher = "Federation of European Neuroscience Societies",
journal = "E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France",
title = "Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats",
pages = "2231-2232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5556"
}

Radovanović, L., Šaponjić, J.,& Petrović, J.. (2022). Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats. in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France
Federation of European Neuroscience Societies., 2231-2232.
https://hdl.handle.net/21.15107/rcub_ibiss_5556

Radovanović L, Šaponjić J, Petrović J. Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats. in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France. 2022;:2231-2232.
https://hdl.handle.net/21.15107/rcub_ibiss_5556 .

Radovanović, Ljiljana, Šaponjić, Jasna, Petrović, Jelena, "Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats" in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France (2022):2231-2232,
https://hdl.handle.net/21.15107/rcub_ibiss_5556 .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0166432820306562
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33038348
UR  - https://radar.ibiss.bg.ac.rs/123456789/3908
AB  - We investigated the prodromal alterations of local sleep, particularly the motor cortical and hippocampal sleep, along with spontaneous locomotor activity in the rat models of Parkinson's disease (PD). We performed our experiments in adult, male Wistar rats, chronically implanted for sleep recording and divided into four experimental groups: the control (implanted controls), the bilateral pedunculopontine tegmental nucleus (PPT) lesions (PD cholinopathy), the unilateral substantia nigra pars compacta (SNpc) lesions (hemiparkinsonism) and the unilateral SNpc/bilateral PPT lesions (hemiparkinsonism with PD cholinopathy). We followed their sleep, basal locomotor activity and spatial habituation for 14 days following the surgical procedures. Severe prodromal local sleep disturbances in the hemiparkinsonian rats were expressed as sleep fragmentation and distinct local NREM/REM EEG microstructure alterations in both the motor cortex and the hippocampus. Alongside the state-unrelated role of the dopaminergic control of theta oscillations and NREM/REM related sigma and beta oscillations, we demonstrated that the REM neurochemical regulatory substrate is particularly important in the dopaminergic control of beta oscillations. In addition, hippocampal prodromal sleep disorders in the hemiparkinsonian rats were expressed as NREM/REM fragmentation and the opposite impact of dopaminergic versus cholinergic control of the NREM delta and beta oscillation amplitudes in the hippocampus, likewise in the motor cortex versus the hippocampus. All these distinct prodromal local sleep disorders and the dopaminergic vs. cholinergic impact on NREM/REM EEG microstructure alterations are of fundamental importance for the further development and follow-up of PD-modifying therapies, and for the identification of patients who are at risk of developing PD.
PB  - Elsevier
T2  - Behavioural Brain Research
T1  - Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.
VL  - 397
DO  - 10.1016/j.bbr.2020.112957
SP  - 112957
ER  - 

@article{
author = "Petrović, Jelena and Radovanović, Ljiljana and Šaponjić, Jasna",
year = "2021",
abstract = "We investigated the prodromal alterations of local sleep, particularly the motor cortical and hippocampal sleep, along with spontaneous locomotor activity in the rat models of Parkinson's disease (PD). We performed our experiments in adult, male Wistar rats, chronically implanted for sleep recording and divided into four experimental groups: the control (implanted controls), the bilateral pedunculopontine tegmental nucleus (PPT) lesions (PD cholinopathy), the unilateral substantia nigra pars compacta (SNpc) lesions (hemiparkinsonism) and the unilateral SNpc/bilateral PPT lesions (hemiparkinsonism with PD cholinopathy). We followed their sleep, basal locomotor activity and spatial habituation for 14 days following the surgical procedures. Severe prodromal local sleep disturbances in the hemiparkinsonian rats were expressed as sleep fragmentation and distinct local NREM/REM EEG microstructure alterations in both the motor cortex and the hippocampus. Alongside the state-unrelated role of the dopaminergic control of theta oscillations and NREM/REM related sigma and beta oscillations, we demonstrated that the REM neurochemical regulatory substrate is particularly important in the dopaminergic control of beta oscillations. In addition, hippocampal prodromal sleep disorders in the hemiparkinsonian rats were expressed as NREM/REM fragmentation and the opposite impact of dopaminergic versus cholinergic control of the NREM delta and beta oscillation amplitudes in the hippocampus, likewise in the motor cortex versus the hippocampus. All these distinct prodromal local sleep disorders and the dopaminergic vs. cholinergic impact on NREM/REM EEG microstructure alterations are of fundamental importance for the further development and follow-up of PD-modifying therapies, and for the identification of patients who are at risk of developing PD.",
publisher = "Elsevier",
journal = "Behavioural Brain Research",
title = "Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.",
volume = "397",
doi = "10.1016/j.bbr.2020.112957",
pages = "112957"
}

Petrović, J., Radovanović, L.,& Šaponjić, J.. (2021). Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.. in Behavioural Brain Research
Elsevier., 397, 112957.
https://doi.org/10.1016/j.bbr.2020.112957

Petrović J, Radovanović L, Šaponjić J. Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.. in Behavioural Brain Research. 2021;397:112957.
doi:10.1016/j.bbr.2020.112957 .

Petrović, Jelena, Radovanović, Ljiljana, Šaponjić, Jasna, "Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy." in Behavioural Brain Research, 397 (2021):112957,
https://doi.org/10.1016/j.bbr.2020.112957 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0166432820306562
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33038348
UR  - https://radar.ibiss.bg.ac.rs/123456789/3908
UR  - https://radar.ibiss.bg.ac.rs/123456789/3909
AB  - We investigated the prodromal alterations of local sleep, particularly the motor cortical and hippocampal sleep, along with spontaneous locomotor activity in the rat models of Parkinson's disease (PD). We performed our experiments in adult, male Wistar rats, chronically implanted for sleep recording and divided into four experimental groups: the control (implanted controls), the bilateral pedunculopontine tegmental nucleus (PPT) lesions (PD cholinopathy), the unilateral substantia nigra pars compacta (SNpc) lesions (hemiparkinsonism) and the unilateral SNpc/bilateral PPT lesions (hemiparkinsonism with PD cholinopathy). We followed their sleep, basal locomotor activity and spatial habituation for 14 days following the surgical procedures. Severe prodromal local sleep disturbances in the hemiparkinsonian rats were expressed as sleep fragmentation and distinct local NREM/REM EEG microstructure alterations in both the motor cortex and the hippocampus. Alongside the state-unrelated role of the dopaminergic control of theta oscillations and NREM/REM related sigma and beta oscillations, we demonstrated that the REM neurochemical regulatory substrate is particularly important in the dopaminergic control of beta oscillations. In addition, hippocampal prodromal sleep disorders in the hemiparkinsonian rats were expressed as NREM/REM fragmentation and the opposite impact of dopaminergic versus cholinergic control of the NREM delta and beta oscillation amplitudes in the hippocampus, likewise in the motor cortex versus the hippocampus. All these distinct prodromal local sleep disorders and the dopaminergic vs. cholinergic impact on NREM/REM EEG microstructure alterations are of fundamental importance for the further development and follow-up of PD-modifying therapies, and for the identification of patients who are at risk of developing PD.
PB  - Elsevier
T2  - Behavioural Brain Research
T1  - Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.
VL  - 397
DO  - 10.1016/j.bbr.2020.112957
SP  - 112957
ER  - 

@article{
author = "Petrović, Jelena and Radovanović, Ljiljana and Šaponjić, Jasna",
year = "2021",
abstract = "We investigated the prodromal alterations of local sleep, particularly the motor cortical and hippocampal sleep, along with spontaneous locomotor activity in the rat models of Parkinson's disease (PD). We performed our experiments in adult, male Wistar rats, chronically implanted for sleep recording and divided into four experimental groups: the control (implanted controls), the bilateral pedunculopontine tegmental nucleus (PPT) lesions (PD cholinopathy), the unilateral substantia nigra pars compacta (SNpc) lesions (hemiparkinsonism) and the unilateral SNpc/bilateral PPT lesions (hemiparkinsonism with PD cholinopathy). We followed their sleep, basal locomotor activity and spatial habituation for 14 days following the surgical procedures. Severe prodromal local sleep disturbances in the hemiparkinsonian rats were expressed as sleep fragmentation and distinct local NREM/REM EEG microstructure alterations in both the motor cortex and the hippocampus. Alongside the state-unrelated role of the dopaminergic control of theta oscillations and NREM/REM related sigma and beta oscillations, we demonstrated that the REM neurochemical regulatory substrate is particularly important in the dopaminergic control of beta oscillations. In addition, hippocampal prodromal sleep disorders in the hemiparkinsonian rats were expressed as NREM/REM fragmentation and the opposite impact of dopaminergic versus cholinergic control of the NREM delta and beta oscillation amplitudes in the hippocampus, likewise in the motor cortex versus the hippocampus. All these distinct prodromal local sleep disorders and the dopaminergic vs. cholinergic impact on NREM/REM EEG microstructure alterations are of fundamental importance for the further development and follow-up of PD-modifying therapies, and for the identification of patients who are at risk of developing PD.",
publisher = "Elsevier",
journal = "Behavioural Brain Research",
title = "Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.",
volume = "397",
doi = "10.1016/j.bbr.2020.112957",
pages = "112957"
}

Petrović, J., Radovanović, L.,& Šaponjić, J.. (2021). Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.. in Behavioural Brain Research
Elsevier., 397, 112957.
https://doi.org/10.1016/j.bbr.2020.112957

Petrović J, Radovanović L, Šaponjić J. Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.. in Behavioural Brain Research. 2021;397:112957.
doi:10.1016/j.bbr.2020.112957 .

Petrović, Jelena, Radovanović, Ljiljana, Šaponjić, Jasna, "Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy." in Behavioural Brain Research, 397 (2021):112957,
https://doi.org/10.1016/j.bbr.2020.112957 . .

TY  - JOUR
AU  - Radovanović, Ljiljana
AU  - Petrović, Jelena
AU  - Šaponjić, Jasna
PY  - 2021
UR  - https://www.mdpi.com/1422-0067/22/16/8922
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4460
AB  - We investigated the alterations of hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV) interneurons and their synaptic re-organizations underlying the prodromal local sleep disorders in the distinct rat models of Parkinson’s disease (PD). We demonstrated for the first time that REM sleep is a predisposing state for the high-voltage sleep spindles (HVS) induction in all experimental models of PD, particularly during hippocampal REM sleep in the hemiparkinsonian models. There were the opposite underlying alterations of the hippocampal and RT GABAergic PV+ interneurons along with the distinct MAP2 and PSD-95 expressions. Whereas the PD cholinopathy enhanced the number of PV+ interneurons and suppressed the MAP2/PSD-95 expression, the hemiparkinsonism with PD cholinopathy reduced the number of PV+ interneurons and enhanced the MAP2/PSD-95 expression in the hippocampus. Whereas the PD cholinopathy did not alter PV+ interneurons but partially enhanced MAP2 and suppressed PSD-95 expression remotely in the RT, the hemiparkinsonism with PD cholinopathy reduced the PV+ interneurons, enhanced MAP2, and did not change PSD-95 expression remotely in the RT. Our study demonstrates for the first time an important regulatory role of the hippocampal and RT GABAergic PV+ interneurons and the synaptic protein dynamic alterations in the distinct rat models of PD neuropathology.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology
IS  - 16
VL  - 22
DO  - 10.3390/ijms22168922
SP  - 8922
ER  - 

@article{
author = "Radovanović, Ljiljana and Petrović, Jelena and Šaponjić, Jasna",
year = "2021",
abstract = "We investigated the alterations of hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV) interneurons and their synaptic re-organizations underlying the prodromal local sleep disorders in the distinct rat models of Parkinson’s disease (PD). We demonstrated for the first time that REM sleep is a predisposing state for the high-voltage sleep spindles (HVS) induction in all experimental models of PD, particularly during hippocampal REM sleep in the hemiparkinsonian models. There were the opposite underlying alterations of the hippocampal and RT GABAergic PV+ interneurons along with the distinct MAP2 and PSD-95 expressions. Whereas the PD cholinopathy enhanced the number of PV+ interneurons and suppressed the MAP2/PSD-95 expression, the hemiparkinsonism with PD cholinopathy reduced the number of PV+ interneurons and enhanced the MAP2/PSD-95 expression in the hippocampus. Whereas the PD cholinopathy did not alter PV+ interneurons but partially enhanced MAP2 and suppressed PSD-95 expression remotely in the RT, the hemiparkinsonism with PD cholinopathy reduced the PV+ interneurons, enhanced MAP2, and did not change PSD-95 expression remotely in the RT. Our study demonstrates for the first time an important regulatory role of the hippocampal and RT GABAergic PV+ interneurons and the synaptic protein dynamic alterations in the distinct rat models of PD neuropathology.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology",
number = "16",
volume = "22",
doi = "10.3390/ijms22168922",
pages = "8922"
}

Radovanović, L., Petrović, J.,& Šaponjić, J.. (2021). Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology. in International Journal of Molecular Sciences
Basel: MDPI., 22(16), 8922.
https://doi.org/10.3390/ijms22168922

Radovanović L, Petrović J, Šaponjić J. Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology. in International Journal of Molecular Sciences. 2021;22(16):8922.
doi:10.3390/ijms22168922 .

Radovanović, Ljiljana, Petrović, Jelena, Šaponjić, Jasna, "Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology" in International Journal of Molecular Sciences, 22, no. 16 (2021):8922,
https://doi.org/10.3390/ijms22168922 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/jsr.13090
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32472657
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3696
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3707
AB  - We investigated the homogeneity/heterogeneity of spontaneous sleep, simultaneously recorded in the motor cortex and the hippocampus of control rats, and particularly analysed simultaneous and non-simultaneous motor cortical and hippocampal non-rapid eye movement (NREM)/rapid eye movement (REM) sleep. We demonstrate that the sleep architectures of the motor cortex and hippocampus are different in control rats. There was an increase of NREM duration and a decrease of REM duration in the hippocampus versus the motor cortex. In terms of duration, NREM state is the most heterogeneous in the hippocampus, whereas the REM state is the most heterogeneous in the motor cortex. Whereas the hippocampal NREM duration was increased due to the prolongation of NREM episodes, the hippocampal REM duration decreased due to the decreased number of REM episodes. The heterogeneity of sleep in the motor cortex and hippocampus in control rats was particularly expressed through the inverse alteration of sigma amplitude during NREM sleep and beta/gamma amplitudes during REM sleep in the hippocampus, along with the delta, sigma, beta and gamma amplitudes only during non-simultaneous NREM/REM sleep in the hippocampus. We demonstrated the brain structure-related and NREM/REM state-related heterogeneity of the motor cortical and hippocampal local sleep in control rats. The distinctly altered local NREM/REM states, alongside their episode dynamics and electroencephalographic (EEG) microstructures, suggest the importance of both the local neuronal network substrate and the NREM/REM neurochemical substrate in the control mechanisms of sleep.
PB  - Blackwell Publishing Ltd
T2  - Journal of Sleep Research
T1  - Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.
DO  - 10.1111/jsr.13090
SP  - e13090
ER  - 

@article{
author = "Petrović, Jelena and Radovanović, Ljiljana and Šaponjić, Jasna",
year = "2020",
abstract = "We investigated the homogeneity/heterogeneity of spontaneous sleep, simultaneously recorded in the motor cortex and the hippocampus of control rats, and particularly analysed simultaneous and non-simultaneous motor cortical and hippocampal non-rapid eye movement (NREM)/rapid eye movement (REM) sleep. We demonstrate that the sleep architectures of the motor cortex and hippocampus are different in control rats. There was an increase of NREM duration and a decrease of REM duration in the hippocampus versus the motor cortex. In terms of duration, NREM state is the most heterogeneous in the hippocampus, whereas the REM state is the most heterogeneous in the motor cortex. Whereas the hippocampal NREM duration was increased due to the prolongation of NREM episodes, the hippocampal REM duration decreased due to the decreased number of REM episodes. The heterogeneity of sleep in the motor cortex and hippocampus in control rats was particularly expressed through the inverse alteration of sigma amplitude during NREM sleep and beta/gamma amplitudes during REM sleep in the hippocampus, along with the delta, sigma, beta and gamma amplitudes only during non-simultaneous NREM/REM sleep in the hippocampus. We demonstrated the brain structure-related and NREM/REM state-related heterogeneity of the motor cortical and hippocampal local sleep in control rats. The distinctly altered local NREM/REM states, alongside their episode dynamics and electroencephalographic (EEG) microstructures, suggest the importance of both the local neuronal network substrate and the NREM/REM neurochemical substrate in the control mechanisms of sleep.",
publisher = "Blackwell Publishing Ltd",
journal = "Journal of Sleep Research",
title = "Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.",
doi = "10.1111/jsr.13090",
pages = "e13090"
}

Petrović, J., Radovanović, L.,& Šaponjić, J.. (2020). Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.. in Journal of Sleep Research
Blackwell Publishing Ltd., e13090.
https://doi.org/10.1111/jsr.13090

Petrović J, Radovanović L, Šaponjić J. Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.. in Journal of Sleep Research. 2020;:e13090.
doi:10.1111/jsr.13090 .

Petrović, Jelena, Radovanović, Ljiljana, Šaponjić, Jasna, "Diversity of simultaneous sleep in the motor cortex and hippocampus in rats." in Journal of Sleep Research (2020):e13090,
https://doi.org/10.1111/jsr.13090 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/jsr.13090
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32472657
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3696
UR  - https://radar.ibiss.bg.ac.rs/handle/handle/123456789/3707
AB  - We investigated the homogeneity/heterogeneity of spontaneous sleep, simultaneously recorded in the motor cortex and the hippocampus of control rats, and particularly analysed simultaneous and non-simultaneous motor cortical and hippocampal non-rapid eye movement (NREM)/rapid eye movement (REM) sleep. We demonstrate that the sleep architectures of the motor cortex and hippocampus are different in control rats. There was an increase of NREM duration and a decrease of REM duration in the hippocampus versus the motor cortex. In terms of duration, NREM state is the most heterogeneous in the hippocampus, whereas the REM state is the most heterogeneous in the motor cortex. Whereas the hippocampal NREM duration was increased due to the prolongation of NREM episodes, the hippocampal REM duration decreased due to the decreased number of REM episodes. The heterogeneity of sleep in the motor cortex and hippocampus in control rats was particularly expressed through the inverse alteration of sigma amplitude during NREM sleep and beta/gamma amplitudes during REM sleep in the hippocampus, along with the delta, sigma, beta and gamma amplitudes only during non-simultaneous NREM/REM sleep in the hippocampus. We demonstrated the brain structure-related and NREM/REM state-related heterogeneity of the motor cortical and hippocampal local sleep in control rats. The distinctly altered local NREM/REM states, alongside their episode dynamics and electroencephalographic (EEG) microstructures, suggest the importance of both the local neuronal network substrate and the NREM/REM neurochemical substrate in the control mechanisms of sleep.
PB  - Blackwell Publishing Ltd
T2  - Journal of Sleep Research
T1  - Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.
DO  - 10.1111/jsr.13090
SP  - e13090
ER  - 

@article{
author = "Petrović, Jelena and Radovanović, Ljiljana and Šaponjić, Jasna",
year = "2020",
abstract = "We investigated the homogeneity/heterogeneity of spontaneous sleep, simultaneously recorded in the motor cortex and the hippocampus of control rats, and particularly analysed simultaneous and non-simultaneous motor cortical and hippocampal non-rapid eye movement (NREM)/rapid eye movement (REM) sleep. We demonstrate that the sleep architectures of the motor cortex and hippocampus are different in control rats. There was an increase of NREM duration and a decrease of REM duration in the hippocampus versus the motor cortex. In terms of duration, NREM state is the most heterogeneous in the hippocampus, whereas the REM state is the most heterogeneous in the motor cortex. Whereas the hippocampal NREM duration was increased due to the prolongation of NREM episodes, the hippocampal REM duration decreased due to the decreased number of REM episodes. The heterogeneity of sleep in the motor cortex and hippocampus in control rats was particularly expressed through the inverse alteration of sigma amplitude during NREM sleep and beta/gamma amplitudes during REM sleep in the hippocampus, along with the delta, sigma, beta and gamma amplitudes only during non-simultaneous NREM/REM sleep in the hippocampus. We demonstrated the brain structure-related and NREM/REM state-related heterogeneity of the motor cortical and hippocampal local sleep in control rats. The distinctly altered local NREM/REM states, alongside their episode dynamics and electroencephalographic (EEG) microstructures, suggest the importance of both the local neuronal network substrate and the NREM/REM neurochemical substrate in the control mechanisms of sleep.",
publisher = "Blackwell Publishing Ltd",
journal = "Journal of Sleep Research",
title = "Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.",
doi = "10.1111/jsr.13090",
pages = "e13090"
}

Petrović, J., Radovanović, L.,& Šaponjić, J.. (2020). Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.. in Journal of Sleep Research
Blackwell Publishing Ltd., e13090.
https://doi.org/10.1111/jsr.13090

Petrović J, Radovanović L, Šaponjić J. Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.. in Journal of Sleep Research. 2020;:e13090.
doi:10.1111/jsr.13090 .

Petrović, Jelena, Radovanović, Ljiljana, Šaponjić, Jasna, "Diversity of simultaneous sleep in the motor cortex and hippocampus in rats." in Journal of Sleep Research (2020):e13090,
https://doi.org/10.1111/jsr.13090 . .

TY  - JOUR
AU  - Vučićević, Ljubica
AU  - Misirkić Marjanović, Maja
AU  - Ćirić, Darko
AU  - Martinović, Tamara
AU  - Jovanović, Maja
AU  - Isaković, Aleksandra
AU  - Marković, Ivanka
AU  - Šaponjić, Jasna
AU  - Foretz, Marc
AU  - Rabanal-Ruiz, Yoana
AU  - Korolchuk, Viktor I.
AU  - Trajković, Vladimir
PY  - 2020
UR  - http://link.springer.com/10.1007/s00018-019-03356-2
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3528
AB  - We investigated the role of autophagy, a controlled lysosomal degradation of cellular macromolecules and organelles, in glutamate excitotoxicity during nutrient deprivation in vitro. The incubation in low-glucose serum/amino acid-free cell culture medium synergized with glutamate in increasing AMP/ATP ratio and causing excitotoxic necrosis in SH-SY5Y human neuroblastoma cells. Glutamate suppressed starvation-triggered autophagy, as confirmed by diminished intracellular acidification, lower LC3 punctuation and LC3-I conversion to autophagosome-associated LC3-II, reduced expression of proautophagic beclin-1 and ATG5, increase of the selective autophagic target NBR1, and decreased number of autophagic vesicles. Similar results were observed in PC12 rat pheochromocytoma cells. Both glutamate-mediated excitotoxicity and autophagy inhibition in starved SH-SY5Y cells were reverted by NMDA antagonist memantine and mimicked by NMDA agonists D-aspartate and ibotenate. Glutamate reduced starvation-triggered phosphorylation of the energy sensor AMP-activated protein kinase (AMPK) without affecting the activity of mammalian target of rapamycin complex 1, a major negative regulator of autophagy. This was associated with reduced mRNA levels of autophagy transcriptional activators (FOXO3, ATF4) and molecules involved in autophagy initiation (ULK1, ATG13, FIP200), autophagosome nucleation/elongation (ATG14, beclin-1, ATG5), and autophagic cargo delivery to autophagosomes (SQSTM1). Glutamate-mediated transcriptional repression of autophagy was alleviated by overexpression of constitutively active AMPK. Genetic or pharmacological AMPK activation by AMPK overexpression or metformin, as well as genetic or pharmacological autophagy induction by TFEB overexpression or lithium chloride, reduced the sensitivity of nutrient-deprived SH-SY5Y cells to glutamate excitotoxicity. These data indicate that transcriptional inhibition of AMPK-dependent cytoprotective autophagy is involved in glutamate-mediated excitotoxicity during nutrient deprivation in vitro.
T2  - Cellular and Molecular Life Sciences
T1  - Transcriptional block of AMPK-induced autophagy promotes glutamate excitotoxicity in nutrient-deprived SH-SY5Y neuroblastoma cells.
VL  - 77
DO  - 10.1007/s00018-019-03356-2
SP  - 3383
EP  - 3399
ER  - 

@article{
author = "Vučićević, Ljubica and Misirkić Marjanović, Maja and Ćirić, Darko and Martinović, Tamara and Jovanović, Maja and Isaković, Aleksandra and Marković, Ivanka and Šaponjić, Jasna and Foretz, Marc and Rabanal-Ruiz, Yoana and Korolchuk, Viktor I. and Trajković, Vladimir",
year = "2020",
abstract = "We investigated the role of autophagy, a controlled lysosomal degradation of cellular macromolecules and organelles, in glutamate excitotoxicity during nutrient deprivation in vitro. The incubation in low-glucose serum/amino acid-free cell culture medium synergized with glutamate in increasing AMP/ATP ratio and causing excitotoxic necrosis in SH-SY5Y human neuroblastoma cells. Glutamate suppressed starvation-triggered autophagy, as confirmed by diminished intracellular acidification, lower LC3 punctuation and LC3-I conversion to autophagosome-associated LC3-II, reduced expression of proautophagic beclin-1 and ATG5, increase of the selective autophagic target NBR1, and decreased number of autophagic vesicles. Similar results were observed in PC12 rat pheochromocytoma cells. Both glutamate-mediated excitotoxicity and autophagy inhibition in starved SH-SY5Y cells were reverted by NMDA antagonist memantine and mimicked by NMDA agonists D-aspartate and ibotenate. Glutamate reduced starvation-triggered phosphorylation of the energy sensor AMP-activated protein kinase (AMPK) without affecting the activity of mammalian target of rapamycin complex 1, a major negative regulator of autophagy. This was associated with reduced mRNA levels of autophagy transcriptional activators (FOXO3, ATF4) and molecules involved in autophagy initiation (ULK1, ATG13, FIP200), autophagosome nucleation/elongation (ATG14, beclin-1, ATG5), and autophagic cargo delivery to autophagosomes (SQSTM1). Glutamate-mediated transcriptional repression of autophagy was alleviated by overexpression of constitutively active AMPK. Genetic or pharmacological AMPK activation by AMPK overexpression or metformin, as well as genetic or pharmacological autophagy induction by TFEB overexpression or lithium chloride, reduced the sensitivity of nutrient-deprived SH-SY5Y cells to glutamate excitotoxicity. These data indicate that transcriptional inhibition of AMPK-dependent cytoprotective autophagy is involved in glutamate-mediated excitotoxicity during nutrient deprivation in vitro.",
journal = "Cellular and Molecular Life Sciences",
title = "Transcriptional block of AMPK-induced autophagy promotes glutamate excitotoxicity in nutrient-deprived SH-SY5Y neuroblastoma cells.",
volume = "77",
doi = "10.1007/s00018-019-03356-2",
pages = "3383-3399"
}

Vučićević, L., Misirkić Marjanović, M., Ćirić, D., Martinović, T., Jovanović, M., Isaković, A., Marković, I., Šaponjić, J., Foretz, M., Rabanal-Ruiz, Y., Korolchuk, V. I.,& Trajković, V.. (2020). Transcriptional block of AMPK-induced autophagy promotes glutamate excitotoxicity in nutrient-deprived SH-SY5Y neuroblastoma cells.. in Cellular and Molecular Life Sciences, 77, 3383-3399.
https://doi.org/10.1007/s00018-019-03356-2

Vučićević L, Misirkić Marjanović M, Ćirić D, Martinović T, Jovanović M, Isaković A, Marković I, Šaponjić J, Foretz M, Rabanal-Ruiz Y, Korolchuk VI, Trajković V. Transcriptional block of AMPK-induced autophagy promotes glutamate excitotoxicity in nutrient-deprived SH-SY5Y neuroblastoma cells.. in Cellular and Molecular Life Sciences. 2020;77:3383-3399.
doi:10.1007/s00018-019-03356-2 .

Vučićević, Ljubica, Misirkić Marjanović, Maja, Ćirić, Darko, Martinović, Tamara, Jovanović, Maja, Isaković, Aleksandra, Marković, Ivanka, Šaponjić, Jasna, Foretz, Marc, Rabanal-Ruiz, Yoana, Korolchuk, Viktor I., Trajković, Vladimir, "Transcriptional block of AMPK-induced autophagy promotes glutamate excitotoxicity in nutrient-deprived SH-SY5Y neuroblastoma cells." in Cellular and Molecular Life Sciences, 77 (2020):3383-3399,
https://doi.org/10.1007/s00018-019-03356-2 . .

TY  - JOUR
AU  - Rajković, Nemanja
AU  - Ćirić, Jelena
AU  - Milošević, Nebojša
AU  - Šaponjić, Jasna
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0010482519303518?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3487
AB  - To reveal the best choice of algorithm for parvalbumin-immunostained images of the hippocampal gyrus dentatus in two distinct rat models of Parkinson's disease (PD), particularly in terms of extracting the crucial information from the image, we tested whether the impact of experimentally induced dopaminergic (hemiparkinsonism) vs. cholinergic (PD cholinopathy) innervation impairment on the parvalbumin stained GABA interneurons could be detected using two separate algorithms, the fractal box-count and the gray-level co-occurrence matrix analysis (GLCM) algorithms. For the texture and fractal analysis of the hippocampal gyrus dentatus images, we used.tif images from three experimental groups of adult male Wistar rats: control rats, rats with Parkinson disease (PD) cholinergic neuropathology (with a PPT lesion), and hemiparkinsonian rats (with a SNpc lesion). For the suprapyramidal layer of the gyrus dentatus ASM and Entropy differentiated the images of the SNpc lesion versus the images of the control and the PPT lesion subjects, with significantly higher ASM and lower Entropy, indicating the homogenization of the images and their lower gray-level complexity. The infrapyramidal images of the SNpc group were differentiated versus the images from the control and PPT groups in terms of all the GLCM parameters: they showed lower mean Entropy and Contrast and higher ASM, Correlation and IDM. These results strongly suggest a rise in the uniformity, homogeneity and orderliness in the gray-levels of images from the SNpc group. Our results indicate that GLCM analysis is a more sensitive tool than fractal analysis for the detection of increased dendritic arborization in histological images.
T2  - Computers in Biology and Medicine
T1  - Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson's disease.
VL  - 115
DO  - 10.1016/j.compbiomed.2019.103482
SP  - 103482
ER  - 

@article{
author = "Rajković, Nemanja and Ćirić, Jelena and Milošević, Nebojša and Šaponjić, Jasna",
year = "2019",
abstract = "To reveal the best choice of algorithm for parvalbumin-immunostained images of the hippocampal gyrus dentatus in two distinct rat models of Parkinson's disease (PD), particularly in terms of extracting the crucial information from the image, we tested whether the impact of experimentally induced dopaminergic (hemiparkinsonism) vs. cholinergic (PD cholinopathy) innervation impairment on the parvalbumin stained GABA interneurons could be detected using two separate algorithms, the fractal box-count and the gray-level co-occurrence matrix analysis (GLCM) algorithms. For the texture and fractal analysis of the hippocampal gyrus dentatus images, we used.tif images from three experimental groups of adult male Wistar rats: control rats, rats with Parkinson disease (PD) cholinergic neuropathology (with a PPT lesion), and hemiparkinsonian rats (with a SNpc lesion). For the suprapyramidal layer of the gyrus dentatus ASM and Entropy differentiated the images of the SNpc lesion versus the images of the control and the PPT lesion subjects, with significantly higher ASM and lower Entropy, indicating the homogenization of the images and their lower gray-level complexity. The infrapyramidal images of the SNpc group were differentiated versus the images from the control and PPT groups in terms of all the GLCM parameters: they showed lower mean Entropy and Contrast and higher ASM, Correlation and IDM. These results strongly suggest a rise in the uniformity, homogeneity and orderliness in the gray-levels of images from the SNpc group. Our results indicate that GLCM analysis is a more sensitive tool than fractal analysis for the detection of increased dendritic arborization in histological images.",
journal = "Computers in Biology and Medicine",
title = "Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson's disease.",
volume = "115",
doi = "10.1016/j.compbiomed.2019.103482",
pages = "103482"
}

Rajković, N., Ćirić, J., Milošević, N.,& Šaponjić, J.. (2019). Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson's disease.. in Computers in Biology and Medicine, 115, 103482.
https://doi.org/10.1016/j.compbiomed.2019.103482

Rajković N, Ćirić J, Milošević N, Šaponjić J. Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson's disease.. in Computers in Biology and Medicine. 2019;115:103482.
doi:10.1016/j.compbiomed.2019.103482 .

Rajković, Nemanja, Ćirić, Jelena, Milošević, Nebojša, Šaponjić, Jasna, "Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson's disease." in Computers in Biology and Medicine, 115 (2019):103482,
https://doi.org/10.1016/j.compbiomed.2019.103482 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Kapor, Slobodan
AU  - Perović, Milka
AU  - Šaponjić, Jasna
PY  - 2019
UR  - https://www.frontiersin.org/article/10.3389/fnins.2019.00148/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6401659
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3294
AB  - Our previous studies in the rat model of Parkinson's disease (PD) cholinopathy demonstrated the sleep-related alterations in electroencephalographic (EEG) oscillations at the cortical and hippocampal levels, cortical drives, and sleep spindles (SSs) as the earliest functional biomarkers preceding hypokinesia. Our aim in this study was to follow the impact of a unilateral substantia nigra pars compacta (SNpc) lesion in rat on the cortical and hippocampal sleep architectures and their EEG microstructures, as well as the cortico-hippocampal synchronizations of EEG oscillations, and the SS and high voltage sleep spindle (HVS) dynamics during NREM and REM sleep. We performed unilateral SNpc lesions using two different concentrations/volumes of 6-hydroxydopamine (6-OHDA; 12 μg/1 μl or 12 μg/2 μl). Whereas the unilateral dopaminergic neuronal loss >50% throughout the overall SNpc rostro-caudal dimension prolonged the Wake state, with no change in the NREM or REM duration, there was a long-lasting theta amplitude augmentation across all sleep states in the motor cortex (MCx), but also in the CA1 hippocampus (Hipp) during both Wake and REM sleep. We demonstrate that SS are the hallmarks of NREM sleep, but that they also occur during REM sleep in the MCx and Hipp of the control rats. Whereas SS are always longer in REM vs. NREM sleep in both structures, they are consistently slower in the Hipp. The dopaminergic neuronal loss increased the density of SS in both structures and shortened them in the MCx during NREM sleep, without changing the intrinsic frequency. Conversely, HVS are the hallmarks of REM sleep in the control rats, slower in the Hipp vs. MCx, and the dopaminergic neuronal loss increased their density in the MCx, but shortened them more consistently in the Hipp during REM sleep. In addition, there was an altered synchronization of the EEG oscillations between the MCx and Hipp in different sleep states, particularly the theta and sigma coherences during REM sleep. We provide novel evidence for the importance of the SNpc dopaminergic innervation in sleep regulation, theta rhythm generation, and SS/HVS dynamics control. We suggest the importance of the underlying REM sleep regulatory substrate to HVS generation and duration and to the cortico-hippocampal synchronizations of EEG oscillations in hemiparkinsonian rats.
T2  - Frontiers in Neuroscience
T1  - Alterations of Sleep and Sleep Oscillations in the Hemiparkinsonian Rat.
VL  - 13
DO  - 10.3389/fnins.2019.00148
SP  - 148
ER  - 

@article{
author = "Ćirić, Jelena and Kapor, Slobodan and Perović, Milka and Šaponjić, Jasna",
year = "2019",
abstract = "Our previous studies in the rat model of Parkinson's disease (PD) cholinopathy demonstrated the sleep-related alterations in electroencephalographic (EEG) oscillations at the cortical and hippocampal levels, cortical drives, and sleep spindles (SSs) as the earliest functional biomarkers preceding hypokinesia. Our aim in this study was to follow the impact of a unilateral substantia nigra pars compacta (SNpc) lesion in rat on the cortical and hippocampal sleep architectures and their EEG microstructures, as well as the cortico-hippocampal synchronizations of EEG oscillations, and the SS and high voltage sleep spindle (HVS) dynamics during NREM and REM sleep. We performed unilateral SNpc lesions using two different concentrations/volumes of 6-hydroxydopamine (6-OHDA; 12 μg/1 μl or 12 μg/2 μl). Whereas the unilateral dopaminergic neuronal loss >50% throughout the overall SNpc rostro-caudal dimension prolonged the Wake state, with no change in the NREM or REM duration, there was a long-lasting theta amplitude augmentation across all sleep states in the motor cortex (MCx), but also in the CA1 hippocampus (Hipp) during both Wake and REM sleep. We demonstrate that SS are the hallmarks of NREM sleep, but that they also occur during REM sleep in the MCx and Hipp of the control rats. Whereas SS are always longer in REM vs. NREM sleep in both structures, they are consistently slower in the Hipp. The dopaminergic neuronal loss increased the density of SS in both structures and shortened them in the MCx during NREM sleep, without changing the intrinsic frequency. Conversely, HVS are the hallmarks of REM sleep in the control rats, slower in the Hipp vs. MCx, and the dopaminergic neuronal loss increased their density in the MCx, but shortened them more consistently in the Hipp during REM sleep. In addition, there was an altered synchronization of the EEG oscillations between the MCx and Hipp in different sleep states, particularly the theta and sigma coherences during REM sleep. We provide novel evidence for the importance of the SNpc dopaminergic innervation in sleep regulation, theta rhythm generation, and SS/HVS dynamics control. We suggest the importance of the underlying REM sleep regulatory substrate to HVS generation and duration and to the cortico-hippocampal synchronizations of EEG oscillations in hemiparkinsonian rats.",
journal = "Frontiers in Neuroscience",
title = "Alterations of Sleep and Sleep Oscillations in the Hemiparkinsonian Rat.",
volume = "13",
doi = "10.3389/fnins.2019.00148",
pages = "148"
}

Ćirić, J., Kapor, S., Perović, M.,& Šaponjić, J.. (2019). Alterations of Sleep and Sleep Oscillations in the Hemiparkinsonian Rat.. in Frontiers in Neuroscience, 13, 148.
https://doi.org/10.3389/fnins.2019.00148

Ćirić J, Kapor S, Perović M, Šaponjić J. Alterations of Sleep and Sleep Oscillations in the Hemiparkinsonian Rat.. in Frontiers in Neuroscience. 2019;13:148.
doi:10.3389/fnins.2019.00148 .

Ćirić, Jelena, Kapor, Slobodan, Perović, Milka, Šaponjić, Jasna, "Alterations of Sleep and Sleep Oscillations in the Hemiparkinsonian Rat." in Frontiers in Neuroscience, 13 (2019):148,
https://doi.org/10.3389/fnins.2019.00148 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Kapor, Slobodan
AU  - Perović, Milka
AU  - Petrović, Jelena
AU  - Pešić, Vesna
AU  - Kanazir, Selma
AU  - Šaponjić, Jasna
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0166432817312391
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29170000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3361
AB  - In order to find out the possible earliest biomarkers of Parkinson's disease (PD) cholinopathy, we followed the impact of bilateral pedunculopontine tegmental nucleus (PPT) lesion in rat on: the cortical and hippocampal sleep/wake states architectures, all sleep states related EEG microstructures, sleep spindles, the basal and stimulated locomotor activity. Sleep and basal locomotor activity in adult Wistar rats were followed during their inactive circadian phase, and throughout the same aging period. The bilateral PPT lesions were done by 0.1M ibotenic acid (IBO) during the surgical procedure for implantation of the electroencephalographic (EEG) and electromyographic (EMG) electrodes for chronic sleep recording. The cholinergic neuronal loss was identified by NADPH - diaphorase histochemistry. After all sleep and behavioral recording sessions, the locomotor activity was stimulated by d-amphetamine (d-AMPH) and the neuronal activity of striatum was followed by c-Fos immunolabeling. Impaired cholinergic innervation from the PPT was expressed earlier as sleep disorder then as movement disorder, and it was the earliest and long-lasting at hippocampal and thalamo-cortical level, and followed by a delayed "hypokinesia". This severe impact of a tonically impaired PPT cholinergic innervation was evidenced as the cholinergic interneuronal loss of the caudate putamen and as a suppressed c-Fos expression after stimulation by d-AMPH. In order how they occurred, the hippocampal non rapid eye movement (NREM) sleep disorder, altered high voltage sleep spindle (HVS) dynamics during rapid eye movement (REM) sleep in the hippocampus and motor cortex, and "hypokinesia" may serve as the biomarkers of PD cholinopathy onset and progression.
T2  - Behavioural Brain Research
T1  - Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat
VL  - 339
DO  - 10.1016/j.bbr.2017.11.021
SP  - 79
EP  - 92
ER  - 

@article{
author = "Ćirić, Jelena and Lazić, Katarina and Kapor, Slobodan and Perović, Milka and Petrović, Jelena and Pešić, Vesna and Kanazir, Selma and Šaponjić, Jasna",
year = "2018",
abstract = "In order to find out the possible earliest biomarkers of Parkinson's disease (PD) cholinopathy, we followed the impact of bilateral pedunculopontine tegmental nucleus (PPT) lesion in rat on: the cortical and hippocampal sleep/wake states architectures, all sleep states related EEG microstructures, sleep spindles, the basal and stimulated locomotor activity. Sleep and basal locomotor activity in adult Wistar rats were followed during their inactive circadian phase, and throughout the same aging period. The bilateral PPT lesions were done by 0.1M ibotenic acid (IBO) during the surgical procedure for implantation of the electroencephalographic (EEG) and electromyographic (EMG) electrodes for chronic sleep recording. The cholinergic neuronal loss was identified by NADPH - diaphorase histochemistry. After all sleep and behavioral recording sessions, the locomotor activity was stimulated by d-amphetamine (d-AMPH) and the neuronal activity of striatum was followed by c-Fos immunolabeling. Impaired cholinergic innervation from the PPT was expressed earlier as sleep disorder then as movement disorder, and it was the earliest and long-lasting at hippocampal and thalamo-cortical level, and followed by a delayed "hypokinesia". This severe impact of a tonically impaired PPT cholinergic innervation was evidenced as the cholinergic interneuronal loss of the caudate putamen and as a suppressed c-Fos expression after stimulation by d-AMPH. In order how they occurred, the hippocampal non rapid eye movement (NREM) sleep disorder, altered high voltage sleep spindle (HVS) dynamics during rapid eye movement (REM) sleep in the hippocampus and motor cortex, and "hypokinesia" may serve as the biomarkers of PD cholinopathy onset and progression.",
journal = "Behavioural Brain Research",
title = "Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat",
volume = "339",
doi = "10.1016/j.bbr.2017.11.021",
pages = "79-92"
}

Ćirić, J., Lazić, K., Kapor, S., Perović, M., Petrović, J., Pešić, V., Kanazir, S.,& Šaponjić, J.. (2018). Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat. in Behavioural Brain Research, 339, 79-92.
https://doi.org/10.1016/j.bbr.2017.11.021

Ćirić J, Lazić K, Kapor S, Perović M, Petrović J, Pešić V, Kanazir S, Šaponjić J. Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat. in Behavioural Brain Research. 2018;339:79-92.
doi:10.1016/j.bbr.2017.11.021 .

Ćirić, Jelena, Lazić, Katarina, Kapor, Slobodan, Perović, Milka, Petrović, Jelena, Pešić, Vesna, Kanazir, Selma, Šaponjić, Jasna, "Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat" in Behavioural Brain Research, 339 (2018):79-92,
https://doi.org/10.1016/j.bbr.2017.11.021 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Kapor, Slobodan
AU  - Perović, Milka
AU  - Petrović, Jelena
AU  - Pešić, Vesna
AU  - Kanazir, Selma
AU  - Šaponjić, Jasna
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0166432817312391
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29170000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2932
AB  - In order to find out the possible earliest biomarkers of Parkinson's disease (PD) cholinopathy, we followed the impact of bilateral pedunculopontine tegmental nucleus (PPT) lesion in rat on: the cortical and hippocampal sleep/wake states architectures, all sleep states related EEG microstructures, sleep spindles, the basal and stimulated locomotor activity. Sleep and basal locomotor activity in adult Wistar rats were followed during their inactive circadian phase, and throughout the same aging period. The bilateral PPT lesions were done by 0.1M ibotenic acid (IBO) during the surgical procedure for implantation of the electroencephalographic (EEG) and electromyographic (EMG) electrodes for chronic sleep recording. The cholinergic neuronal loss was identified by NADPH - diaphorase histochemistry. After all sleep and behavioral recording sessions, the locomotor activity was stimulated by d-amphetamine (d-AMPH) and the neuronal activity of striatum was followed by c-Fos immunolabeling. Impaired cholinergic innervation from the PPT was expressed earlier as sleep disorder then as movement disorder, and it was the earliest and long-lasting at hippocampal and thalamo-cortical level, and followed by a delayed "hypokinesia". This severe impact of a tonically impaired PPT cholinergic innervation was evidenced as the cholinergic interneuronal loss of the caudate putamen and as a suppressed c-Fos expression after stimulation by d-AMPH. In order how they occurred, the hippocampal non rapid eye movement (NREM) sleep disorder, altered high voltage sleep spindle (HVS) dynamics during rapid eye movement (REM) sleep in the hippocampus and motor cortex, and "hypokinesia" may serve as the biomarkers of PD cholinopathy onset and progression.
T2  - Behavioural Brain Research
T1  - Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat
VL  - 339
DO  - 10.1016/j.bbr.2017.11.021
SP  - 79
EP  - 92
ER  - 

@article{
author = "Ćirić, Jelena and Lazić, Katarina and Kapor, Slobodan and Perović, Milka and Petrović, Jelena and Pešić, Vesna and Kanazir, Selma and Šaponjić, Jasna",
year = "2018",
abstract = "In order to find out the possible earliest biomarkers of Parkinson's disease (PD) cholinopathy, we followed the impact of bilateral pedunculopontine tegmental nucleus (PPT) lesion in rat on: the cortical and hippocampal sleep/wake states architectures, all sleep states related EEG microstructures, sleep spindles, the basal and stimulated locomotor activity. Sleep and basal locomotor activity in adult Wistar rats were followed during their inactive circadian phase, and throughout the same aging period. The bilateral PPT lesions were done by 0.1M ibotenic acid (IBO) during the surgical procedure for implantation of the electroencephalographic (EEG) and electromyographic (EMG) electrodes for chronic sleep recording. The cholinergic neuronal loss was identified by NADPH - diaphorase histochemistry. After all sleep and behavioral recording sessions, the locomotor activity was stimulated by d-amphetamine (d-AMPH) and the neuronal activity of striatum was followed by c-Fos immunolabeling. Impaired cholinergic innervation from the PPT was expressed earlier as sleep disorder then as movement disorder, and it was the earliest and long-lasting at hippocampal and thalamo-cortical level, and followed by a delayed "hypokinesia". This severe impact of a tonically impaired PPT cholinergic innervation was evidenced as the cholinergic interneuronal loss of the caudate putamen and as a suppressed c-Fos expression after stimulation by d-AMPH. In order how they occurred, the hippocampal non rapid eye movement (NREM) sleep disorder, altered high voltage sleep spindle (HVS) dynamics during rapid eye movement (REM) sleep in the hippocampus and motor cortex, and "hypokinesia" may serve as the biomarkers of PD cholinopathy onset and progression.",
journal = "Behavioural Brain Research",
title = "Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat",
volume = "339",
doi = "10.1016/j.bbr.2017.11.021",
pages = "79-92"
}

Ćirić, J., Lazić, K., Kapor, S., Perović, M., Petrović, J., Pešić, V., Kanazir, S.,& Šaponjić, J.. (2018). Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat. in Behavioural Brain Research, 339, 79-92.
https://doi.org/10.1016/j.bbr.2017.11.021

Ćirić J, Lazić K, Kapor S, Perović M, Petrović J, Pešić V, Kanazir S, Šaponjić J. Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat. in Behavioural Brain Research. 2018;339:79-92.
doi:10.1016/j.bbr.2017.11.021 .

Ćirić, Jelena, Lazić, Katarina, Kapor, Slobodan, Perović, Milka, Petrović, Jelena, Pešić, Vesna, Kanazir, Selma, Šaponjić, Jasna, "Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat" in Behavioural Brain Research, 339 (2018):79-92,
https://doi.org/10.1016/j.bbr.2017.11.021 . .

TY  - JOUR
AU  - Lazić, Katarina
AU  - Ćirić, Jelena
AU  - Šaponjić, Jasna
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jsr.12758
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3140
AB  - On the basis of our previous studies and the important role of the thalamo-cortical network in states of unconsciousness, such as anaesthesia and sleep, and in sleep spindles generation, we investigated sleep spindles (SS) and high-voltage sleep spindle (HVS) dynamics during non-rapid eye movement (NREM) and rapid eye movement (REM) sleep following different types of general anaesthesia in both physiological controls and in a rat model of Parkinson's disease (PD) cholinopathy, to follow the impact of anaesthesia on post-anaesthesia sleep at the thalamo-cortical level through an altered sleep spindle dynamics. We recorded 6 hr of spontaneous sleep in all rats, both before and 48 hr after ketamine/diazepam or pentobarbital anaesthesia, and we used 1 hr of NREM or REM sleep from each to validate visually the automatically detected SS or HVS for their extraction and analysis. In the controls, SS occurred mainly during NREM, whereas HVS occurred only during REM sleep. Ketamine/diazepam anaesthesia promoted HVS, prolonged SS during NREM, induced HVS of increased frequency during REM, and increased SS/HVS densities during REM versus NREM sleep. Pentobarbital anaesthesia decreased the frequency of SS during NREM and the HVS density during REM sleep. Although the pedunculopontine tegmental nucleus lesion prolonged SS only during NREM sleep, in these rats, ketamine/diazepam anaesthesia suppressed HVS during both sleep states, whereas pentobarbital anaesthesia promoted HVS during REM sleep. The different impacts of two anaesthetic regimens on the thalamo-cortical regulatory network are expressed through their distinct sleep spindle generation and dynamics that are dependent on the NREM and REM state regulatory neuronal substrate.
T2  - Journal of Sleep Research
T1  - Sleep spindle dynamics during NREM and REM sleep following distinct general anaesthesia in control rats and in a rat model of Parkinson’s disease cholinopathy
DO  - 10.1111/jsr.12758
ER  - 

@article{
author = "Lazić, Katarina and Ćirić, Jelena and Šaponjić, Jasna",
year = "2018",
abstract = "On the basis of our previous studies and the important role of the thalamo-cortical network in states of unconsciousness, such as anaesthesia and sleep, and in sleep spindles generation, we investigated sleep spindles (SS) and high-voltage sleep spindle (HVS) dynamics during non-rapid eye movement (NREM) and rapid eye movement (REM) sleep following different types of general anaesthesia in both physiological controls and in a rat model of Parkinson's disease (PD) cholinopathy, to follow the impact of anaesthesia on post-anaesthesia sleep at the thalamo-cortical level through an altered sleep spindle dynamics. We recorded 6 hr of spontaneous sleep in all rats, both before and 48 hr after ketamine/diazepam or pentobarbital anaesthesia, and we used 1 hr of NREM or REM sleep from each to validate visually the automatically detected SS or HVS for their extraction and analysis. In the controls, SS occurred mainly during NREM, whereas HVS occurred only during REM sleep. Ketamine/diazepam anaesthesia promoted HVS, prolonged SS during NREM, induced HVS of increased frequency during REM, and increased SS/HVS densities during REM versus NREM sleep. Pentobarbital anaesthesia decreased the frequency of SS during NREM and the HVS density during REM sleep. Although the pedunculopontine tegmental nucleus lesion prolonged SS only during NREM sleep, in these rats, ketamine/diazepam anaesthesia suppressed HVS during both sleep states, whereas pentobarbital anaesthesia promoted HVS during REM sleep. The different impacts of two anaesthetic regimens on the thalamo-cortical regulatory network are expressed through their distinct sleep spindle generation and dynamics that are dependent on the NREM and REM state regulatory neuronal substrate.",
journal = "Journal of Sleep Research",
title = "Sleep spindle dynamics during NREM and REM sleep following distinct general anaesthesia in control rats and in a rat model of Parkinson’s disease cholinopathy",
doi = "10.1111/jsr.12758"
}

Lazić, K., Ćirić, J.,& Šaponjić, J.. (2018). Sleep spindle dynamics during NREM and REM sleep following distinct general anaesthesia in control rats and in a rat model of Parkinson’s disease cholinopathy. in Journal of Sleep Research.
https://doi.org/10.1111/jsr.12758

Lazić K, Ćirić J, Šaponjić J. Sleep spindle dynamics during NREM and REM sleep following distinct general anaesthesia in control rats and in a rat model of Parkinson’s disease cholinopathy. in Journal of Sleep Research. 2018;.
doi:10.1111/jsr.12758 .

Lazić, Katarina, Ćirić, Jelena, Šaponjić, Jasna, "Sleep spindle dynamics during NREM and REM sleep following distinct general anaesthesia in control rats and in a rat model of Parkinson’s disease cholinopathy" in Journal of Sleep Research (2018),
https://doi.org/10.1111/jsr.12758 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Milosevic, Vuk
AU  - Živković, Miroslava
AU  - Stojanov, Dragan
AU  - Milojković, Olga
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2017
UR  - https://peerj.com/articles/3839
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2874
AB  - Background. We investigated EEG rhythms, particularly alpha activity, and their relationship to post-stroke neuropathology and cognitive functions in the subacute and chronic stages of minor strokes. Methods. We included 10 patients with right middle cerebral artery (MCA) ischemic strokes a nd 11 healthy controls. All the assessments of stroke patients were done both in the subacute and chronic stages. Neurological impairment was measured using the National Institute of Health Stroke Scale (NIHSS), whereas cognitive functions were assessed using the Montreal Cognitive Assessment (MoCA) and MoCA memory index (MoCA-MIS). The EEG was recorded using a 19 channel EEG system with standard EEG electrode placement. In particular, we analyzed the EEGs derived from the four lateral frontal (F3, F7, F4, F8), and corresponding lateral posterior (P3, P4, T5, T6) electrodes. Quantitative EEG analysis included: the group FFT spectra, the weighted average of alpha frequency (α AVG), the group probability density distributions of all conventional EEG frequency band relative amplitudes (EEG microstructure), the inter- and intra-hemispheric coherences, and the topographic distribution of alpha carrier frequency phase potentials (PPs). Statistical analysis was done using a Kruskal-Wallis ANOVA with a post-hoc Mann-WhitneyU two-tailed test, and Spearman's correlation. Results. We demonstrated transient cognitive impairment alongside a slower alpha fre- quency (αAVG) in the subacute right MCA stroke patients vs. the controls. This slower alpha frequency showed no amplitude change, but was highly synchronized intra- hemispherically, overlying the ipsi-lesional hemisphere, and inter-hemispherically, overlying the frontal cortex. In addition, the disturbances in EEG alpha activity in subacute stroke patients were expressed as a decrease in alpha PPs over the frontal cortex and an altered "alpha flow", indicating the sustained augmentation of inter- hemispheric interactions. Although the stroke induced slower alpha was a transient phenomenon, the increased alpha intra-hemispheric synchronization, overlying the ipsi-lesional hemisphere, the increased alpha F3-F4 inter-hemispheric synchronization, the delayed alpha waves, and the newly established inter-hemispheric "alpha flow" within the frontal cortex, remained as a permanent consequence of the minor stroke. This newly established frontal inter-hemispheric "alpha flow" represented a permanent consequence of the ``hidden" stroke neuropathology, despite the fact that cognitive impairment has been returned to the control values. All the detected permanent changes at the EEG level with no cognitive impairment after a minor stroke could be a way for the brain to compensate for the lesion and restore the lost function. Discussion. Our study indicates slower EEG alpha generation, synchronization and ``flow" as potential biomarkers of cognitive impairment onset and/or compensatory post-stroke re-organizational processes.
T2  - PeerJ
T1  - Slower EEG alpha generation, synchronization and “flow”—possible biomarkers of cognitive impairment and neuropathology of minor stroke
IS  - 9
VL  - 5
DO  - 10.7717/peerj.3839
SP  - e3839
EP  - e3839
ER  - 

@article{
author = "Petrović, Jelena and Milosevic, Vuk and Živković, Miroslava and Stojanov, Dragan and Milojković, Olga and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2017",
abstract = "Background. We investigated EEG rhythms, particularly alpha activity, and their relationship to post-stroke neuropathology and cognitive functions in the subacute and chronic stages of minor strokes. Methods. We included 10 patients with right middle cerebral artery (MCA) ischemic strokes a nd 11 healthy controls. All the assessments of stroke patients were done both in the subacute and chronic stages. Neurological impairment was measured using the National Institute of Health Stroke Scale (NIHSS), whereas cognitive functions were assessed using the Montreal Cognitive Assessment (MoCA) and MoCA memory index (MoCA-MIS). The EEG was recorded using a 19 channel EEG system with standard EEG electrode placement. In particular, we analyzed the EEGs derived from the four lateral frontal (F3, F7, F4, F8), and corresponding lateral posterior (P3, P4, T5, T6) electrodes. Quantitative EEG analysis included: the group FFT spectra, the weighted average of alpha frequency (α AVG), the group probability density distributions of all conventional EEG frequency band relative amplitudes (EEG microstructure), the inter- and intra-hemispheric coherences, and the topographic distribution of alpha carrier frequency phase potentials (PPs). Statistical analysis was done using a Kruskal-Wallis ANOVA with a post-hoc Mann-WhitneyU two-tailed test, and Spearman's correlation. Results. We demonstrated transient cognitive impairment alongside a slower alpha fre- quency (αAVG) in the subacute right MCA stroke patients vs. the controls. This slower alpha frequency showed no amplitude change, but was highly synchronized intra- hemispherically, overlying the ipsi-lesional hemisphere, and inter-hemispherically, overlying the frontal cortex. In addition, the disturbances in EEG alpha activity in subacute stroke patients were expressed as a decrease in alpha PPs over the frontal cortex and an altered "alpha flow", indicating the sustained augmentation of inter- hemispheric interactions. Although the stroke induced slower alpha was a transient phenomenon, the increased alpha intra-hemispheric synchronization, overlying the ipsi-lesional hemisphere, the increased alpha F3-F4 inter-hemispheric synchronization, the delayed alpha waves, and the newly established inter-hemispheric "alpha flow" within the frontal cortex, remained as a permanent consequence of the minor stroke. This newly established frontal inter-hemispheric "alpha flow" represented a permanent consequence of the ``hidden" stroke neuropathology, despite the fact that cognitive impairment has been returned to the control values. All the detected permanent changes at the EEG level with no cognitive impairment after a minor stroke could be a way for the brain to compensate for the lesion and restore the lost function. Discussion. Our study indicates slower EEG alpha generation, synchronization and ``flow" as potential biomarkers of cognitive impairment onset and/or compensatory post-stroke re-organizational processes.",
journal = "PeerJ",
title = "Slower EEG alpha generation, synchronization and “flow”—possible biomarkers of cognitive impairment and neuropathology of minor stroke",
number = "9",
volume = "5",
doi = "10.7717/peerj.3839",
pages = "e3839-e3839"
}

Petrović, J., Milosevic, V., Živković, M., Stojanov, D., Milojković, O., Kalauzi, A.,& Šaponjić, J.. (2017). Slower EEG alpha generation, synchronization and “flow”—possible biomarkers of cognitive impairment and neuropathology of minor stroke. in PeerJ, 5(9), e3839-e3839.
https://doi.org/10.7717/peerj.3839

Petrović J, Milosevic V, Živković M, Stojanov D, Milojković O, Kalauzi A, Šaponjić J. Slower EEG alpha generation, synchronization and “flow”—possible biomarkers of cognitive impairment and neuropathology of minor stroke. in PeerJ. 2017;5(9):e3839-e3839.
doi:10.7717/peerj.3839 .

Petrović, Jelena, Milosevic, Vuk, Živković, Miroslava, Stojanov, Dragan, Milojković, Olga, Kalauzi, Aleksandar, Šaponjić, Jasna, "Slower EEG alpha generation, synchronization and “flow”—possible biomarkers of cognitive impairment and neuropathology of minor stroke" in PeerJ, 5, no. 9 (2017):e3839-e3839,
https://doi.org/10.7717/peerj.3839 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Petrović, Jelena
AU  - Kalauzi, A
AU  - Šaponjić, Jasna
PY  - 2017
UR  - http://www.oatext.com/sleep-spindles-as-an-early-biomarker-of-rem-sleep-disorder-in-a-rat-model-of-parkinsons-disease-cholinopathy.php
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3296
AB  - Rhythmic oscillations of neuronal populations, generated by different mechanisms, are present at several levels of the central nervous system and serve many important physiological or reflect pathological functions. Understanding the role of brain oscillations as possible biomarkers of brain function and plasticity is still a challenge, and despite extensive research, their role is still not well established. We recently demonstrated that the hallmarks of earlier aging onset during impaired thalamo-cortical cholinergic innervation (in a rat model of Parkinson’s disease cholinopathy) were consistently expressed, from 3 and one half to 5 and one half months of age, through increased electroencephalographic (EEG) sigma activity amplitude during rapid eye movement (REM) sleep, as a unique aging induced REM sleep phenomenon. In addition, there was altered motor cortical drive during non-rapid-eye-movement (NREM) and REM sleep. In order to explain this new aging-induced REM sleep phenomenon, we analyzed possible differences between control REM sleep spindle activity and REM sleep spindle activity at the onset of REM sleep “enriched“ with sigma activity (at 4 and one half months of age), following bilateral pedunculopontine tegmental nucleus (PPT) cholinergic neuronal loss in the rat. We analzyed differences in spindle density, duration, and frequency. We demonstrated in young adult Wistar rats with the severely impaired PPT cholinergic innervation the alterations in sleep spindle dynamics and pattern during REM sleep in the motor cortex as the earliest biomarkers for the onset of their altered aging processes.
T2  - Translational Brain Rhythmicity
T1  - Sleep spindles as an early biomarker of REM sleep disorder in a rat model of Parkinson’s disease cholinopathy
IS  - 1
VL  - 2
DO  - 10.15761/TBR.1000111
SP  - 1
EP  - 11
ER  - 

@article{
author = "Ćirić, Jelena and Lazić, Katarina and Petrović, Jelena and Kalauzi, A and Šaponjić, Jasna",
year = "2017",
abstract = "Rhythmic oscillations of neuronal populations, generated by different mechanisms, are present at several levels of the central nervous system and serve many important physiological or reflect pathological functions. Understanding the role of brain oscillations as possible biomarkers of brain function and plasticity is still a challenge, and despite extensive research, their role is still not well established. We recently demonstrated that the hallmarks of earlier aging onset during impaired thalamo-cortical cholinergic innervation (in a rat model of Parkinson’s disease cholinopathy) were consistently expressed, from 3 and one half to 5 and one half months of age, through increased electroencephalographic (EEG) sigma activity amplitude during rapid eye movement (REM) sleep, as a unique aging induced REM sleep phenomenon. In addition, there was altered motor cortical drive during non-rapid-eye-movement (NREM) and REM sleep. In order to explain this new aging-induced REM sleep phenomenon, we analyzed possible differences between control REM sleep spindle activity and REM sleep spindle activity at the onset of REM sleep “enriched“ with sigma activity (at 4 and one half months of age), following bilateral pedunculopontine tegmental nucleus (PPT) cholinergic neuronal loss in the rat. We analzyed differences in spindle density, duration, and frequency. We demonstrated in young adult Wistar rats with the severely impaired PPT cholinergic innervation the alterations in sleep spindle dynamics and pattern during REM sleep in the motor cortex as the earliest biomarkers for the onset of their altered aging processes.",
journal = "Translational Brain Rhythmicity",
title = "Sleep spindles as an early biomarker of REM sleep disorder in a rat model of Parkinson’s disease cholinopathy",
number = "1",
volume = "2",
doi = "10.15761/TBR.1000111",
pages = "1-11"
}

Ćirić, J., Lazić, K., Petrović, J., Kalauzi, A.,& Šaponjić, J.. (2017). Sleep spindles as an early biomarker of REM sleep disorder in a rat model of Parkinson’s disease cholinopathy. in Translational Brain Rhythmicity, 2(1), 1-11.
https://doi.org/10.15761/TBR.1000111

Ćirić J, Lazić K, Petrović J, Kalauzi A, Šaponjić J. Sleep spindles as an early biomarker of REM sleep disorder in a rat model of Parkinson’s disease cholinopathy. in Translational Brain Rhythmicity. 2017;2(1):1-11.
doi:10.15761/TBR.1000111 .

Ćirić, Jelena, Lazić, Katarina, Petrović, Jelena, Kalauzi, A, Šaponjić, Jasna, "Sleep spindles as an early biomarker of REM sleep disorder in a rat model of Parkinson’s disease cholinopathy" in Translational Brain Rhythmicity, 2, no. 1 (2017):1-11,
https://doi.org/10.15761/TBR.1000111 . .

TY  - JOUR
AU  - Lazić, Katarina
AU  - Petrović, Jelena
AU  - Ćirić, Jelena
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0031938416306308
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3297
AB  - Postoperative sleep disorders, particularly the REM sleep disorder, may have a significant deleterious impact on postoperative outcomes and may contribute to the genesis of certain delayed postoperative complications. We have followed the effect of distinct anesthesia regimens (ketamine/diazepam vs. pentobarbital) over 6days following the induction of a stable anesthetized state in adult male Wistar rats, chronically instrumented for sleep recording. In order to compare the effect of both anesthetics in the physiological controls vs. the rats with impaired pedunculopontine tegmental nucleus (PPT) cholinergic innervation, during the operative procedure for the implantation of EEG and EMG electrodes, the bilateral PPT lesion was conducted using ibotenic acid (IBO). We have followed in particular post-anesthesia REM sleep. Our results show the distinct EEG microstructure of the motor cortex during the different stable anesthetized states, and their distinct impact on post-anesthesia REM sleep. In contrast to pentobarbital anesthesia, the ketamine/diazepam anesthesia potentiated the long-lasting post-anesthesia REM statewith higher muscle tone (REM1) vs. REM state with atonia (REM2). Whereas both anesthesias prolonged the post-anesthesia REM sleep duration, the long-term prolongation of the REM1 state was demonstrated only after the ketamine/diazepam anesthesia, first due to the increased number of REM1 episodes, and then due to the prolonged REM1 episodes duration. On the other hand, whereas both anesthetic regimens abolished the prolonged post-anesthesia REM/REM1 sleep and the EEG microstructure disorder during REM sleep, only the pentobarbital abolished the increased NREM/REM/NREM transitions, caused by the PPT lesion. In addition, in the PPT lesioned rats, the ketamine/diazepam anesthesia decreased the Wake/NREM/Wake transitions while the pentobarbital anesthesia decreased the Wake/REM/Wake transitions. Our present study suggests pentobarbital anesthesia as being highly beneficial for post-anesthesia REM sleep in the physiological condition as well as during PPT cholinergic neuropathology.
T2  - Physiology & Behavior
T1  - REM sleep disorder following general anesthesia in rats.
VL  - 168
DO  - 10.1016/j.physbeh.2016.10.013
SP  - 41
EP  - 54
ER  - 

@article{
author = "Lazić, Katarina and Petrović, Jelena and Ćirić, Jelena and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2017",
abstract = "Postoperative sleep disorders, particularly the REM sleep disorder, may have a significant deleterious impact on postoperative outcomes and may contribute to the genesis of certain delayed postoperative complications. We have followed the effect of distinct anesthesia regimens (ketamine/diazepam vs. pentobarbital) over 6days following the induction of a stable anesthetized state in adult male Wistar rats, chronically instrumented for sleep recording. In order to compare the effect of both anesthetics in the physiological controls vs. the rats with impaired pedunculopontine tegmental nucleus (PPT) cholinergic innervation, during the operative procedure for the implantation of EEG and EMG electrodes, the bilateral PPT lesion was conducted using ibotenic acid (IBO). We have followed in particular post-anesthesia REM sleep. Our results show the distinct EEG microstructure of the motor cortex during the different stable anesthetized states, and their distinct impact on post-anesthesia REM sleep. In contrast to pentobarbital anesthesia, the ketamine/diazepam anesthesia potentiated the long-lasting post-anesthesia REM statewith higher muscle tone (REM1) vs. REM state with atonia (REM2). Whereas both anesthesias prolonged the post-anesthesia REM sleep duration, the long-term prolongation of the REM1 state was demonstrated only after the ketamine/diazepam anesthesia, first due to the increased number of REM1 episodes, and then due to the prolonged REM1 episodes duration. On the other hand, whereas both anesthetic regimens abolished the prolonged post-anesthesia REM/REM1 sleep and the EEG microstructure disorder during REM sleep, only the pentobarbital abolished the increased NREM/REM/NREM transitions, caused by the PPT lesion. In addition, in the PPT lesioned rats, the ketamine/diazepam anesthesia decreased the Wake/NREM/Wake transitions while the pentobarbital anesthesia decreased the Wake/REM/Wake transitions. Our present study suggests pentobarbital anesthesia as being highly beneficial for post-anesthesia REM sleep in the physiological condition as well as during PPT cholinergic neuropathology.",
journal = "Physiology & Behavior",
title = "REM sleep disorder following general anesthesia in rats.",
volume = "168",
doi = "10.1016/j.physbeh.2016.10.013",
pages = "41-54"
}

Lazić, K., Petrović, J., Ćirić, J., Kalauzi, A.,& Šaponjić, J.. (2017). REM sleep disorder following general anesthesia in rats.. in Physiology & Behavior, 168, 41-54.
https://doi.org/10.1016/j.physbeh.2016.10.013

Lazić K, Petrović J, Ćirić J, Kalauzi A, Šaponjić J. REM sleep disorder following general anesthesia in rats.. in Physiology & Behavior. 2017;168:41-54.
doi:10.1016/j.physbeh.2016.10.013 .

Lazić, Katarina, Petrović, Jelena, Ćirić, Jelena, Kalauzi, Aleksandar, Šaponjić, Jasna, "REM sleep disorder following general anesthesia in rats." in Physiology & Behavior, 168 (2017):41-54,
https://doi.org/10.1016/j.physbeh.2016.10.013 . .

TY  - CHAP
AU  - Šaponjić, Jasna
AU  - Petrović, Jelena
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
PY  - 2016
UR  - http://dx.doi.org/10.5772/62949
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2474
UR  - http://www.intechopen.com/books/challenges-in-parkinson-s-disease/disorders-of-sleep-and-motor-control-during-the-impaired-cholinergic-innervation-in-rat-relevance-to
AB  - The medical profession has been generally very slow to acknowledge the importance of sleep medicine and sleep research. Disorders of sleep are related to anxiety, many mental and neurodegenerative diseases, cardiovascular and respiratory disorders, and obesity. Our knowledge of the neural substrates of sleep/wake states and sleep-related behavior disorders regulation in health and the diseases, over more than 50 years of sleep research, is based on the experiments in animal models, pharmacotherapy, and the neuropathological studies in humans. But, we still need further work in fundamental multidisciplinary and clinical research between sleep and neurodegenerative disease investigators to understand normal and abnormal sleep, and to provide new insights into preventive or disease-altering approaches for therapy. Our aim is to give an overview of our recent results related to the importance of thalamo-cortical cholinergic brain system in the disorders of sleep and motor control during sleep, with particular relevance to Parkinson’s disease.
PB  - Rijeka: InTech
T2  - Challenges in Parkinson’s Disease
T1  - Disorders of Sleep and Motor Control During the Impaired Cholinergic Innervation in Rat – Relevance to Parkinson’s Disease
DO  - 10.5772/62949
SP  - 136
EP  - 153
ER  - 

@inbook{
author = "Šaponjić, Jasna and Petrović, Jelena and Ćirić, Jelena and Lazić, Katarina",
year = "2016",
abstract = "The medical profession has been generally very slow to acknowledge the importance of sleep medicine and sleep research. Disorders of sleep are related to anxiety, many mental and neurodegenerative diseases, cardiovascular and respiratory disorders, and obesity. Our knowledge of the neural substrates of sleep/wake states and sleep-related behavior disorders regulation in health and the diseases, over more than 50 years of sleep research, is based on the experiments in animal models, pharmacotherapy, and the neuropathological studies in humans. But, we still need further work in fundamental multidisciplinary and clinical research between sleep and neurodegenerative disease investigators to understand normal and abnormal sleep, and to provide new insights into preventive or disease-altering approaches for therapy. Our aim is to give an overview of our recent results related to the importance of thalamo-cortical cholinergic brain system in the disorders of sleep and motor control during sleep, with particular relevance to Parkinson’s disease.",
publisher = "Rijeka: InTech",
journal = "Challenges in Parkinson’s Disease",
booktitle = "Disorders of Sleep and Motor Control During the Impaired Cholinergic Innervation in Rat – Relevance to Parkinson’s Disease",
doi = "10.5772/62949",
pages = "136-153"
}

Šaponjić, J., Petrović, J., Ćirić, J.,& Lazić, K.. (2016). Disorders of Sleep and Motor Control During the Impaired Cholinergic Innervation in Rat – Relevance to Parkinson’s Disease. in Challenges in Parkinson’s Disease
Rijeka: InTech., 136-153.
https://doi.org/10.5772/62949

Šaponjić J, Petrović J, Ćirić J, Lazić K. Disorders of Sleep and Motor Control During the Impaired Cholinergic Innervation in Rat – Relevance to Parkinson’s Disease. in Challenges in Parkinson’s Disease. 2016;:136-153.
doi:10.5772/62949 .

Šaponjić, Jasna, Petrović, Jelena, Ćirić, Jelena, Lazić, Katarina, "Disorders of Sleep and Motor Control During the Impaired Cholinergic Innervation in Rat – Relevance to Parkinson’s Disease" in Challenges in Parkinson’s Disease (2016):136-153,
https://doi.org/10.5772/62949 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Petrović, Jelena
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1909
UR  - http://www.sciencedirect.com/science/article/pii/S0166432815303454
AB  - We studied the impact of aging during sleep in the rat models of
   Alzheimer's (AD) and Parkinson's (PD) disease cholinergic neuropathology
   to determine the possible different and earlier onset of age-related
   sleep disorder during the neurodegenerative diseases vs. healthy aging.
   We used the bilateral nucleus basalis (NB) and pedunculopontine
   tegmental nucleus (PPT) lesioned rats as the in vivo models of
   functionally distinct cholinergic neuropathology, and we followed the
   impact of aging on sleep architecture, the electroencephalographic (EEG)
   microstructure and motor control across sleep/wake states.
   Our results have shown for the first time that the earliest signs of
   aging during distinct cholinergic neuropathology were expressed through
   a different and topographically specific EEG microstructure during rapid
   eye movement sleep (REM). EEG delta amplitude attenuation within the
   sensorimotor cortex (SMCx) during REM was the earliest sign of aging in
   the NB lesion. EEG sigma amplitude augmentation within the motor cortex
   (MCx) during REM was the earliest sign of aging in the PPT lesion. In
   addition, aging was differently expressed through the SMCx drive
   alterations, but it was commonly expressed through the MCx drive
   alterations during all sleep/wake states.
   Our study provided evidence of distinct REM sleep disorders and sleep
   state related cortical drives as the signs of aging onset during
   functionally distinct cholinergic neuropathologies (NB lesion vs. PPT
   lesion). (C) 2015 Elsevier B.V. All rights reserved.
T2  - Behavioural Brain Research
T1  - Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology
VL  - 301
DO  - 10.1016/j.bbr.2015.12.046
SP  - 273
EP  - 286
ER  - 

@article{
author = "Ćirić, Jelena and Lazić, Katarina and Petrović, Jelena and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2016",
abstract = "We studied the impact of aging during sleep in the rat models of
   Alzheimer's (AD) and Parkinson's (PD) disease cholinergic neuropathology
   to determine the possible different and earlier onset of age-related
   sleep disorder during the neurodegenerative diseases vs. healthy aging.
   We used the bilateral nucleus basalis (NB) and pedunculopontine
   tegmental nucleus (PPT) lesioned rats as the in vivo models of
   functionally distinct cholinergic neuropathology, and we followed the
   impact of aging on sleep architecture, the electroencephalographic (EEG)
   microstructure and motor control across sleep/wake states.
   Our results have shown for the first time that the earliest signs of
   aging during distinct cholinergic neuropathology were expressed through
   a different and topographically specific EEG microstructure during rapid
   eye movement sleep (REM). EEG delta amplitude attenuation within the
   sensorimotor cortex (SMCx) during REM was the earliest sign of aging in
   the NB lesion. EEG sigma amplitude augmentation within the motor cortex
   (MCx) during REM was the earliest sign of aging in the PPT lesion. In
   addition, aging was differently expressed through the SMCx drive
   alterations, but it was commonly expressed through the MCx drive
   alterations during all sleep/wake states.
   Our study provided evidence of distinct REM sleep disorders and sleep
   state related cortical drives as the signs of aging onset during
   functionally distinct cholinergic neuropathologies (NB lesion vs. PPT
   lesion). (C) 2015 Elsevier B.V. All rights reserved.",
journal = "Behavioural Brain Research",
title = "Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology",
volume = "301",
doi = "10.1016/j.bbr.2015.12.046",
pages = "273-286"
}

Ćirić, J., Lazić, K., Petrović, J., Kalauzi, A.,& Šaponjić, J.. (2016). Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology. in Behavioural Brain Research, 301, 273-286.
https://doi.org/10.1016/j.bbr.2015.12.046

Ćirić J, Lazić K, Petrović J, Kalauzi A, Šaponjić J. Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology. in Behavioural Brain Research. 2016;301:273-286.
doi:10.1016/j.bbr.2015.12.046 .

Ćirić, Jelena, Lazić, Katarina, Petrović, Jelena, Kalauzi, Aleksandar, Šaponjić, Jasna, "Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology" in Behavioural Brain Research, 301 (2016):273-286,
https://doi.org/10.1016/j.bbr.2015.12.046 . .

TY  - JOUR
AU  - Ciric, Jelena
AU  - Lazic, Katarina
AU  - Petrovic, Jelena
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1990
AB  - We followed the impact of healthy aging on cortical drive during sleep
   in rats by using the corticomuscular coherence (CMC).
   We employed the chronic electrodes implantation for sleep recording in
   adult, male Wistar rats, and followed the aging impact during sleep from
   3 to 5.5 months age. We have analyzed the sleep/wake states
   architecture, and the sleep/wake state related EEG microstructure and
   CMCs.
   We evidenced the topographically distinct impact of aging on sleep/wake
   states architecture within the sensorimotor (SMCx) vs. motor cortex
   (MCx) from 4.5 to 5.5 months age. Healthy aging consistently altered
   only the SMCx sleep/wake states architecture, and increased the delta
   and beta CMCs through both cortical drives during Wake, but only through
   the MCx drive during REM. According to the delta and beta CMCs values,
   aging impact through the SMCx drive was opposite, but it was convergent
   through the MCx drive during Wake vs. REM, and there was a dual and
   inverse mode for the motor control during REM. (C) 2015 Elsevier Ireland
   Ltd. All rights reserved.
T2  - Mechanisms of Ageing and Development
T1  - Aging induced cortical drive alterations during sleep in rats
VL  - 146
DO  - 10.1016/j.mad.2015.03.002
SP  - 12
EP  - 22
ER  - 

@article{
author = "Ciric, Jelena and Lazic, Katarina and Petrovic, Jelena and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2015",
abstract = "We followed the impact of healthy aging on cortical drive during sleep
   in rats by using the corticomuscular coherence (CMC).
   We employed the chronic electrodes implantation for sleep recording in
   adult, male Wistar rats, and followed the aging impact during sleep from
   3 to 5.5 months age. We have analyzed the sleep/wake states
   architecture, and the sleep/wake state related EEG microstructure and
   CMCs.
   We evidenced the topographically distinct impact of aging on sleep/wake
   states architecture within the sensorimotor (SMCx) vs. motor cortex
   (MCx) from 4.5 to 5.5 months age. Healthy aging consistently altered
   only the SMCx sleep/wake states architecture, and increased the delta
   and beta CMCs through both cortical drives during Wake, but only through
   the MCx drive during REM. According to the delta and beta CMCs values,
   aging impact through the SMCx drive was opposite, but it was convergent
   through the MCx drive during Wake vs. REM, and there was a dual and
   inverse mode for the motor control during REM. (C) 2015 Elsevier Ireland
   Ltd. All rights reserved.",
journal = "Mechanisms of Ageing and Development",
title = "Aging induced cortical drive alterations during sleep in rats",
volume = "146",
doi = "10.1016/j.mad.2015.03.002",
pages = "12-22"
}

Ciric, J., Lazic, K., Petrovic, J., Kalauzi, A.,& Šaponjić, J.. (2015). Aging induced cortical drive alterations during sleep in rats. in Mechanisms of Ageing and Development, 146, 12-22.
https://doi.org/10.1016/j.mad.2015.03.002

Ciric J, Lazic K, Petrovic J, Kalauzi A, Šaponjić J. Aging induced cortical drive alterations during sleep in rats. in Mechanisms of Ageing and Development. 2015;146:12-22.
doi:10.1016/j.mad.2015.03.002 .

Ciric, Jelena, Lazic, Katarina, Petrovic, Jelena, Kalauzi, Aleksandar, Šaponjić, Jasna, "Aging induced cortical drive alterations during sleep in rats" in Mechanisms of Ageing and Development, 146 (2015):12-22,
https://doi.org/10.1016/j.mad.2015.03.002 . .

TY  - JOUR
AU  - Lazic, K.
AU  - Petrovic, J.
AU  - Ciric, J.
AU  - Kalauzi, A.
AU  - Šaponjić, Jasna
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2361
AB  - Objectives: We hypothesized that the impact of distinct anesthetic
   regimens could be differently expressed during anesthesia and on
   post-anesthesia sleep in the neurodegenerative diseases. Therefore, we
   followed the impact of ketamine/diazepam and pentobarbital anesthesia in
   a rat model of the severe Parkinson's disease cholinergic neuropathology
   on the electroencephalographic (EEG) microstructure and respiratory
   pattern during anesthesia, and on the post-anesthesia sleep. Methods: We
   performed the experiments on adult, male, spontaneously breathing Wistar
   rats chronically instrumented for sleep recording. The bilateral
   pedunculopontine tegmental nucleus (PPT) lesion was done by ibotenic
   acid microinfusion. Following postoperative recovery, we recorded sleep
   for 6 h, induced anesthesia 24 h later using ketamine/diazepam or
   pentobarbital, and repeated sleep recordings sessions 48 h and 6 days
   later. During 20 min of each anesthesia we recorded both the EEG and
   respiratory movements. For sleep and EEG analysis, Fourier analysis was
   applied on 6-h recordings, and each 10-s epoch was differentiated as a
   state of wakefulness (Wake), non-rapid eye movement (NREM) or rapid eye
   movement (REM). Additionally, the group probability density
   distributions of all EEG frequency band relative amplitudes were
   calculated for each state, with particular attention during anesthesia.
   For respiratory pattern analysis we used Monotone Signal Segments
   Analysis. The PPT lesion was identified through nicotinamide adenine
   dinucleotide phosphate (NADPH) diaphorase histochemistry. Results and
   conclusions: Our data show that the ketamine/diazepam anesthetic regimen
   in the PPT-lesioned rats induces more alterations in the EEG
   microstructure and respiratory pattern than does the pentobarbital
   anesthesia. In addition, the equal time required to establish an
   anesthetized state, and the long-term effect on postanesthesia sleep in
   the PPT-lesioned vs. control rats suggest this anesthetic regimen as
   potentially more beneficial both for anesthesia induction and for
   post-anesthesia sleep in the surgical procedures of the elderly, and
   Parkinson's, and Alzheimer's patients. (C) 2015 IBRO. Published by
   Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - Impact of anesthetic regimen on the respiratory pattern, EEG microstructure and sleep in the rat model of cholinergic Parkinson's disease neuropathology
VL  - 304
DO  - 10.1016/j.neuroscience.2015.07.020
SP  - 1
EP  - 13
ER  - 

@article{
author = "Lazic, K. and Petrovic, J. and Ciric, J. and Kalauzi, A. and Šaponjić, Jasna",
year = "2015",
abstract = "Objectives: We hypothesized that the impact of distinct anesthetic
   regimens could be differently expressed during anesthesia and on
   post-anesthesia sleep in the neurodegenerative diseases. Therefore, we
   followed the impact of ketamine/diazepam and pentobarbital anesthesia in
   a rat model of the severe Parkinson's disease cholinergic neuropathology
   on the electroencephalographic (EEG) microstructure and respiratory
   pattern during anesthesia, and on the post-anesthesia sleep. Methods: We
   performed the experiments on adult, male, spontaneously breathing Wistar
   rats chronically instrumented for sleep recording. The bilateral
   pedunculopontine tegmental nucleus (PPT) lesion was done by ibotenic
   acid microinfusion. Following postoperative recovery, we recorded sleep
   for 6 h, induced anesthesia 24 h later using ketamine/diazepam or
   pentobarbital, and repeated sleep recordings sessions 48 h and 6 days
   later. During 20 min of each anesthesia we recorded both the EEG and
   respiratory movements. For sleep and EEG analysis, Fourier analysis was
   applied on 6-h recordings, and each 10-s epoch was differentiated as a
   state of wakefulness (Wake), non-rapid eye movement (NREM) or rapid eye
   movement (REM). Additionally, the group probability density
   distributions of all EEG frequency band relative amplitudes were
   calculated for each state, with particular attention during anesthesia.
   For respiratory pattern analysis we used Monotone Signal Segments
   Analysis. The PPT lesion was identified through nicotinamide adenine
   dinucleotide phosphate (NADPH) diaphorase histochemistry. Results and
   conclusions: Our data show that the ketamine/diazepam anesthetic regimen
   in the PPT-lesioned rats induces more alterations in the EEG
   microstructure and respiratory pattern than does the pentobarbital
   anesthesia. In addition, the equal time required to establish an
   anesthetized state, and the long-term effect on postanesthesia sleep in
   the PPT-lesioned vs. control rats suggest this anesthetic regimen as
   potentially more beneficial both for anesthesia induction and for
   post-anesthesia sleep in the surgical procedures of the elderly, and
   Parkinson's, and Alzheimer's patients. (C) 2015 IBRO. Published by
   Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "Impact of anesthetic regimen on the respiratory pattern, EEG microstructure and sleep in the rat model of cholinergic Parkinson's disease neuropathology",
volume = "304",
doi = "10.1016/j.neuroscience.2015.07.020",
pages = "1-13"
}

Lazic, K., Petrovic, J., Ciric, J., Kalauzi, A.,& Šaponjić, J.. (2015). Impact of anesthetic regimen on the respiratory pattern, EEG microstructure and sleep in the rat model of cholinergic Parkinson's disease neuropathology. in Neuroscience, 304, 1-13.
https://doi.org/10.1016/j.neuroscience.2015.07.020

Lazic K, Petrovic J, Ciric J, Kalauzi A, Šaponjić J. Impact of anesthetic regimen on the respiratory pattern, EEG microstructure and sleep in the rat model of cholinergic Parkinson's disease neuropathology. in Neuroscience. 2015;304:1-13.
doi:10.1016/j.neuroscience.2015.07.020 .

Lazic, K., Petrovic, J., Ciric, J., Kalauzi, A., Šaponjić, Jasna, "Impact of anesthetic regimen on the respiratory pattern, EEG microstructure and sleep in the rat model of cholinergic Parkinson's disease neuropathology" in Neuroscience, 304 (2015):1-13,
https://doi.org/10.1016/j.neuroscience.2015.07.020 . .