TY  - JOUR
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
AU  - Motyka, Vaclav
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/15/1982
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9370364
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5103
AB  - This study presents the hypocotyl elongation of sunflower seedlings germinated under different light conditions. Elongation was rhythmic under diurnal (LD) photoperiods but uniform (arrhythmic) under free-running conditions of white light (LL) or darkness (DD). On the sixth day after the onset of germination, seedlings were entrained in all diurnal photoperiods. Their hypocotyl elongation was dual, showing different kinetics in daytime and nighttime periods. The daytime elongation peak was around midday and 1-2 h after dusk in the nighttime. Plantlets compensated for the differences in the daytime and nighttime durations and exhibited similar overall elongation rates, centered around the uniform elongation in LL conditions. Thus, plants from diurnal photoperiods and LL could be grouped together as white-light treatments that suppressed hypocotyl elongation. Hypocotyl elongation was significantly higher under DD than under white-light photoperiods. In continuous monochromatic blue, yellow, green, or red light, hypocotyl elongation was also uniform and very high. The treatments with monochromatic light and DD had similar overall elongation rates; thus, they could be grouped together. Compared with white light, monochromatic light promoted hypocotyl elongation. Suppression of hypocotyl elongation and rhythmicity reappeared in some combination with two or more monochromatic light colors. The presence of red light was obligatory for this suppression. Plantlets entrained in diurnal photoperiods readily slipped from rhythmic into uniform elongation if they encountered any kind of free-running conditions. These transitions occurred whenever the anticipated duration of daytime or nighttime was extended more than expected, or when plantlets were exposed to constant monochromatic light. This study revealed significant differences in the development of sunflower plantlets illuminated with monochromatic or white light.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings.
IS  - 15
VL  - 11
DO  - 10.3390/plants11151982
SP  - 1982
ER  - 

@article{
author = "Vinterhalter, Dragan and Vinterhalter, Branka and Motyka, Vaclav",
year = "2022",
abstract = "This study presents the hypocotyl elongation of sunflower seedlings germinated under different light conditions. Elongation was rhythmic under diurnal (LD) photoperiods but uniform (arrhythmic) under free-running conditions of white light (LL) or darkness (DD). On the sixth day after the onset of germination, seedlings were entrained in all diurnal photoperiods. Their hypocotyl elongation was dual, showing different kinetics in daytime and nighttime periods. The daytime elongation peak was around midday and 1-2 h after dusk in the nighttime. Plantlets compensated for the differences in the daytime and nighttime durations and exhibited similar overall elongation rates, centered around the uniform elongation in LL conditions. Thus, plants from diurnal photoperiods and LL could be grouped together as white-light treatments that suppressed hypocotyl elongation. Hypocotyl elongation was significantly higher under DD than under white-light photoperiods. In continuous monochromatic blue, yellow, green, or red light, hypocotyl elongation was also uniform and very high. The treatments with monochromatic light and DD had similar overall elongation rates; thus, they could be grouped together. Compared with white light, monochromatic light promoted hypocotyl elongation. Suppression of hypocotyl elongation and rhythmicity reappeared in some combination with two or more monochromatic light colors. The presence of red light was obligatory for this suppression. Plantlets entrained in diurnal photoperiods readily slipped from rhythmic into uniform elongation if they encountered any kind of free-running conditions. These transitions occurred whenever the anticipated duration of daytime or nighttime was extended more than expected, or when plantlets were exposed to constant monochromatic light. This study revealed significant differences in the development of sunflower plantlets illuminated with monochromatic or white light.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings.",
number = "15",
volume = "11",
doi = "10.3390/plants11151982",
pages = "1982"
}

Vinterhalter, D., Vinterhalter, B.,& Motyka, V.. (2022). Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings.. in Plants (Basel, Switzerland)
Basel: MDPI., 11(15), 1982.
https://doi.org/10.3390/plants11151982

Vinterhalter D, Vinterhalter B, Motyka V. Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings.. in Plants (Basel, Switzerland). 2022;11(15):1982.
doi:10.3390/plants11151982 .

Vinterhalter, Dragan, Vinterhalter, Branka, Motyka, Vaclav, "Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings." in Plants (Basel, Switzerland), 11, no. 15 (2022):1982,
https://doi.org/10.3390/plants11151982 . .

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Pavlović, Suzana
AU  - Živković, Suzana
AU  - Vinterhalter, Dragan
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5412
AB  - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
PB  - East Sarajevo : Faculty of Agriculture
PB  - eas
C3  - Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
T1  - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5412
ER  - 

@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena",
year = "2021",
abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.",
publisher = "East Sarajevo : Faculty of Agriculture, eas",
journal = "Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina",
title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)",
pages = "213",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5412"
}

Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
East Sarajevo : Faculty of Agriculture., 213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412

Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina. 2021;:213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina (2021):213,
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

TY  - JOUR
AU  - Krstić Milošević, Dijana
AU  - Banjac, Nevena
AU  - Janković, Teodora
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4310
AB  - In vitro shoot culture of the endangered medicinal plant Gentianella lutescens was established from epicotyl explants cultured on MS basal medium with 0.2 mg L−1 6-benzylaminopurine (BA) and evaluated for xanthones content for the first time. Five shoot lines were obtained and no significant variations in multiplication rate, shoot elongation, and xanthones profile were found among them. The highest rooting rate (33.3%) was achieved by shoots treated for 2 days with 5 mg L−1 indole-3-butyric acid (IBA) followed by cultivation in liquid PGR-free ½ MS medium for 60 days. HPLC analysis revealed the lower content of xanthones—mangiferin, bellidifolin, demethylbellidifolin, demethylbellidifolin-8-O-glucoside and bellidifolin-8-O-glucoside—in in vitro cultured shoots compared to wild growing plants. The increasing concentration of sucrose, sorbitol and abiotic elicitors salicylic acid (SA), jasmonic acid (JA) and methyl jasmonate (MeJA) altered shoot growth and xanthone production. Sucrose and sorbitol applied at the highest concentration of 233.6 mM increased dry matter percentage, while SA at 100 μM promoted shoot growth 2-fold. The increased sucrose concentration enhanced accumulation of xanthones in shoot cultures 2–3-fold compared to the control shoots. Elicitors at 100–300 μM increased the accumulation of mangiferin, demethylbellidifolin-8-O-glucoside, and bellidifolin-8-O-glucoside almost equally, while MeJA at the highest concentration of 500 μM enhanced amount of aglycones demethylbellidifolin and bellidifolin 7-fold compared to the control. The obtained results facilitate conservation of G. lutescens and pave the way for further research on large-scale shoot propagation and production of pharmacologically active xanthones.
PB  - Basel: MDPI
T2  - Plants
T1  - Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production
IS  - 8
VL  - 10
DO  - 10.3390/plants10081651
SP  - 1651
ER  - 

@article{
author = "Krstić Milošević, Dijana and Banjac, Nevena and Janković, Teodora and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2021",
abstract = "In vitro shoot culture of the endangered medicinal plant Gentianella lutescens was established from epicotyl explants cultured on MS basal medium with 0.2 mg L−1 6-benzylaminopurine (BA) and evaluated for xanthones content for the first time. Five shoot lines were obtained and no significant variations in multiplication rate, shoot elongation, and xanthones profile were found among them. The highest rooting rate (33.3%) was achieved by shoots treated for 2 days with 5 mg L−1 indole-3-butyric acid (IBA) followed by cultivation in liquid PGR-free ½ MS medium for 60 days. HPLC analysis revealed the lower content of xanthones—mangiferin, bellidifolin, demethylbellidifolin, demethylbellidifolin-8-O-glucoside and bellidifolin-8-O-glucoside—in in vitro cultured shoots compared to wild growing plants. The increasing concentration of sucrose, sorbitol and abiotic elicitors salicylic acid (SA), jasmonic acid (JA) and methyl jasmonate (MeJA) altered shoot growth and xanthone production. Sucrose and sorbitol applied at the highest concentration of 233.6 mM increased dry matter percentage, while SA at 100 μM promoted shoot growth 2-fold. The increased sucrose concentration enhanced accumulation of xanthones in shoot cultures 2–3-fold compared to the control shoots. Elicitors at 100–300 μM increased the accumulation of mangiferin, demethylbellidifolin-8-O-glucoside, and bellidifolin-8-O-glucoside almost equally, while MeJA at the highest concentration of 500 μM enhanced amount of aglycones demethylbellidifolin and bellidifolin 7-fold compared to the control. The obtained results facilitate conservation of G. lutescens and pave the way for further research on large-scale shoot propagation and production of pharmacologically active xanthones.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production",
number = "8",
volume = "10",
doi = "10.3390/plants10081651",
pages = "1651"
}

Krstić Milošević, D., Banjac, N., Janković, T., Vinterhalter, D.,& Vinterhalter, B.. (2021). Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production. in Plants
Basel: MDPI., 10(8), 1651.
https://doi.org/10.3390/plants10081651

Krstić Milošević D, Banjac N, Janković T, Vinterhalter D, Vinterhalter B. Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production. in Plants. 2021;10(8):1651.
doi:10.3390/plants10081651 .

Krstić Milošević, Dijana, Banjac, Nevena, Janković, Teodora, Vinterhalter, Dragan, Vinterhalter, Branka, "Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production" in Plants, 10, no. 8 (2021):1651,
https://doi.org/10.3390/plants10081651 . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Banjac, Nevena
AU  - Vinterhalter, Dragan
AU  - Krstić Milošević, Dijana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4309
AB  - The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.
PB  - Basel: MDPI
T2  - Plants
T1  - Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors
IS  - 8
VL  - 10
DO  - 10.3390/plants10081610
SP  - 1610
ER  - 

@article{
author = "Vinterhalter, Branka and Banjac, Nevena and Vinterhalter, Dragan and Krstić Milošević, Dijana",
year = "2021",
abstract = "The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors",
number = "8",
volume = "10",
doi = "10.3390/plants10081610",
pages = "1610"
}

Vinterhalter, B., Banjac, N., Vinterhalter, D.,& Krstić Milošević, D.. (2021). Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors. in Plants
Basel: MDPI., 10(8), 1610.
https://doi.org/10.3390/plants10081610

Vinterhalter B, Banjac N, Vinterhalter D, Krstić Milošević D. Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors. in Plants. 2021;10(8):1610.
doi:10.3390/plants10081610 .

Vinterhalter, Branka, Banjac, Nevena, Vinterhalter, Dragan, Krstić Milošević, Dijana, "Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors" in Plants, 10, no. 8 (2021):1610,
https://doi.org/10.3390/plants10081610 . .

TY  - JOUR
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4384
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6234
AB  - Research on phototropic (PT) bending in sunflower (Helianthus annuus L. cv. Kondi (Syngenta)) seedling hypocotyls presented herein focused on a comparison of diurnal and free-running photoperiods with the aim of explaining the development of diurnal rhythmicity. PT bending magnitudes and lag phase duration exhibited strong daily rhythmicity in all diurnal photoperiods, contrasting with the uniform PT bending response to constant light (CL) conditions. Plants had a daytime maximum for PT bending magnitudes in experiments starting around midday and a minimum in the dark period in those starting 4 h after dusk. Plants could compensate for large differences in the daytime duration of diurnal photoperiods. They required the first 4 h of darkness to recover and synchronize the PT bending and to start increasing the magnitudes of PT bending. The daily pattern of lag phase duration changes was similar but inverted, showing that synchronization also occurred during nighttime. Darkness was not required for PT bending under CL conditions, however, during diurnal photoperiods it enabled the establishment of diurnal rhythmicity and synchronized changes in PT bending capacity to occur when needed, providing maximal values at midday and minimal during the nighttime. Under prolonged duration of daytime corresponding to the start of CL condition, plantlets rapidly abandoned circadian regulation, their PT bending response becoming arrhythmic.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Diurnal photoperiods and rhythmicity of the phototropic bending response in hypocotyls of sunflower, Helianthus annuus L. seedlings.
IS  - 2
VL  - 73
DO  - 10.2298/ABS210205018V
SP  - 237
EP  - 246
ER  - 

@article{
author = "Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2021",
abstract = "Research on phototropic (PT) bending in sunflower (Helianthus annuus L. cv. Kondi (Syngenta)) seedling hypocotyls presented herein focused on a comparison of diurnal and free-running photoperiods with the aim of explaining the development of diurnal rhythmicity. PT bending magnitudes and lag phase duration exhibited strong daily rhythmicity in all diurnal photoperiods, contrasting with the uniform PT bending response to constant light (CL) conditions. Plants had a daytime maximum for PT bending magnitudes in experiments starting around midday and a minimum in the dark period in those starting 4 h after dusk. Plants could compensate for large differences in the daytime duration of diurnal photoperiods. They required the first 4 h of darkness to recover and synchronize the PT bending and to start increasing the magnitudes of PT bending. The daily pattern of lag phase duration changes was similar but inverted, showing that synchronization also occurred during nighttime. Darkness was not required for PT bending under CL conditions, however, during diurnal photoperiods it enabled the establishment of diurnal rhythmicity and synchronized changes in PT bending capacity to occur when needed, providing maximal values at midday and minimal during the nighttime. Under prolonged duration of daytime corresponding to the start of CL condition, plantlets rapidly abandoned circadian regulation, their PT bending response becoming arrhythmic.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Diurnal photoperiods and rhythmicity of the phototropic bending response in hypocotyls of sunflower, Helianthus annuus L. seedlings.",
number = "2",
volume = "73",
doi = "10.2298/ABS210205018V",
pages = "237-246"
}

Vinterhalter, D.,& Vinterhalter, B.. (2021). Diurnal photoperiods and rhythmicity of the phototropic bending response in hypocotyls of sunflower, Helianthus annuus L. seedlings.. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 73(2), 237-246.
https://doi.org/10.2298/ABS210205018V

Vinterhalter D, Vinterhalter B. Diurnal photoperiods and rhythmicity of the phototropic bending response in hypocotyls of sunflower, Helianthus annuus L. seedlings.. in Archives of Biological Sciences. 2021;73(2):237-246.
doi:10.2298/ABS210205018V .

Vinterhalter, Dragan, Vinterhalter, Branka, "Diurnal photoperiods and rhythmicity of the phototropic bending response in hypocotyls of sunflower, Helianthus annuus L. seedlings." in Archives of Biological Sciences, 73, no. 2 (2021):237-246,
https://doi.org/10.2298/ABS210205018V . .

TY  - JOUR
AU  - Vinterhalter, Dragan
AU  - Savić, Jelena
AU  - Stanišić, Mariana
AU  - Vinterhalter, Branka
AU  - Dobrev, P. I.
AU  - Motyka, V.
PY  - 2020
UR  - https://link.springer.com/article/10.1007/s10725-019-00561-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3533
AB  - Taking advantage of advanced high performance liquid chromatography–electrospray tandem–mass spectrometry (HPLC–ESI–MS/MS), we screened daily changes in concentrations of endogenous phytohormones of in vitro grown potato (Solanum tuberosum L. cv. Désirée) shoot cultures and checked for possible connections between the rhythmicity of endogenous phytohormones and phototropic bending capacity of the same cultures. Studies done under diurnal 16 h light and 8 h darkness (diurnal) and continuous light (CL) conditions showed prominent daily rhythmicity of endogenous phytohormone levels in both light regimes. Phototropic bending in potato is known to be rhythmic only in the diurnal, whereas in CL conditions the bending response is present but without any daily rhythmicity. For all of the studied phytohormone groups significant differences between the diurnal and CL conditions were found. Changes in the concentration of indole auxins, indole-3-acetic acid (IAA) and its catabolite 2-oxindole-3-acetic acid (OxIAA), were the most prominent. Their levels clearly alternated with level of IAA being high in diurnal and OxIAA in CL conditions. Significant concentration changes were also observed for other phytohormones such as cytokinin ribosides, salicylic acid, abscisic acid and phaseic acid. Observed changes in daily phytohormone levels indicate strong and complex involvement of diverse phytohormone groups in realization of the phototropic bending response of potato shoots.
PB  - Springer Netherlands
T2  - Plant Growth Regulation
T1  - Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures
IS  - 161
VL  - 90
DO  - 10.1007/s10725-019-00561-8
SP  - 151
ER  - 

@article{
author = "Vinterhalter, Dragan and Savić, Jelena and Stanišić, Mariana and Vinterhalter, Branka and Dobrev, P. I. and Motyka, V.",
year = "2020",
abstract = "Taking advantage of advanced high performance liquid chromatography–electrospray tandem–mass spectrometry (HPLC–ESI–MS/MS), we screened daily changes in concentrations of endogenous phytohormones of in vitro grown potato (Solanum tuberosum L. cv. Désirée) shoot cultures and checked for possible connections between the rhythmicity of endogenous phytohormones and phototropic bending capacity of the same cultures. Studies done under diurnal 16 h light and 8 h darkness (diurnal) and continuous light (CL) conditions showed prominent daily rhythmicity of endogenous phytohormone levels in both light regimes. Phototropic bending in potato is known to be rhythmic only in the diurnal, whereas in CL conditions the bending response is present but without any daily rhythmicity. For all of the studied phytohormone groups significant differences between the diurnal and CL conditions were found. Changes in the concentration of indole auxins, indole-3-acetic acid (IAA) and its catabolite 2-oxindole-3-acetic acid (OxIAA), were the most prominent. Their levels clearly alternated with level of IAA being high in diurnal and OxIAA in CL conditions. Significant concentration changes were also observed for other phytohormones such as cytokinin ribosides, salicylic acid, abscisic acid and phaseic acid. Observed changes in daily phytohormone levels indicate strong and complex involvement of diverse phytohormone groups in realization of the phototropic bending response of potato shoots.",
publisher = "Springer Netherlands",
journal = "Plant Growth Regulation",
title = "Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures",
number = "161",
volume = "90",
doi = "10.1007/s10725-019-00561-8",
pages = "151"
}

Vinterhalter, D., Savić, J., Stanišić, M., Vinterhalter, B., Dobrev, P. I.,& Motyka, V.. (2020). Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures. in Plant Growth Regulation
Springer Netherlands., 90(161), 151.
https://doi.org/10.1007/s10725-019-00561-8

Vinterhalter D, Savić J, Stanišić M, Vinterhalter B, Dobrev PI, Motyka V. Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures. in Plant Growth Regulation. 2020;90(161):151.
doi:10.1007/s10725-019-00561-8 .

Vinterhalter, Dragan, Savić, Jelena, Stanišić, Mariana, Vinterhalter, Branka, Dobrev, P. I., Motyka, V., "Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures" in Plant Growth Regulation, 90, no. 161 (2020):151,
https://doi.org/10.1007/s10725-019-00561-8 . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
AU  - Zdravković-Korać, Snežana
AU  - Banjac, Nevena
AU  - Vinterhalter, Dragan
AU  - Krstić Milošević, Dijana
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0926669018311282?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3230
AB  - Production of innovative drugs from natural products in controlled conditions plays an important role in modern pharmacology in order to tackle global health challenges. The potential of Gentiana utriculosa hairy roots, obtained by transformation with Agrobacterium rhizogenes A4M70GUS, as well as that of the shoots regenerated from them via somatic embryogenesis, for xanthone production was investigated. Gentiana utriculosa was shown to be a new source of xanthones, medicinal raw materials for different pharmaceutical applications, among which decussatin has been recently recognized as a prospective hepatoprotective and antiulcer compound. Decussatin and decussatin-1-O-primeveroside were detected in both hairy roots and transgenic shoots, while mangiferin, present in nontransformed plants, was not detected. Quantitative HPLC analysis revealed up to 4.5-fold higher decussatin production in hairy roots line 9 compared with the plants from the nature, and this clone was selected. It showed stable growth after more than two years of continuous subcultivation. Cultivation of hairy roots on solid medium was favorable for biomass production, while liquid culture was beneficial for decussatin and decussatin-1-O-primeveroside accumulation. Cytokinins kinetin or N6-benzyladenine promoted somatic embryo maturation and germination as well as multiplication of obtained plantlets, while active charcoal reduced hyperhydricity. Plants regenerated from selected hairy root line 9 comprised two TL-DNA inserts, as confirmed by Southern blot analysis. They accumulated at least 2.5-fold more decussatin then nontransformed plants. Therefore, these plants could be valuable material to create xanthone high-yielding cultivars of G. utriculosa.
T2  - Industrial Crops and Products
T1  - Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants
VL  - 130
DO  - 10.1016/J.INDCROP.2018.12.066
SP  - 216
EP  - 229
ER  - 

@article{
author = "Vinterhalter, Branka and Savić, Jelena and Zdravković-Korać, Snežana and Banjac, Nevena and Vinterhalter, Dragan and Krstić Milošević, Dijana",
year = "2019",
abstract = "Production of innovative drugs from natural products in controlled conditions plays an important role in modern pharmacology in order to tackle global health challenges. The potential of Gentiana utriculosa hairy roots, obtained by transformation with Agrobacterium rhizogenes A4M70GUS, as well as that of the shoots regenerated from them via somatic embryogenesis, for xanthone production was investigated. Gentiana utriculosa was shown to be a new source of xanthones, medicinal raw materials for different pharmaceutical applications, among which decussatin has been recently recognized as a prospective hepatoprotective and antiulcer compound. Decussatin and decussatin-1-O-primeveroside were detected in both hairy roots and transgenic shoots, while mangiferin, present in nontransformed plants, was not detected. Quantitative HPLC analysis revealed up to 4.5-fold higher decussatin production in hairy roots line 9 compared with the plants from the nature, and this clone was selected. It showed stable growth after more than two years of continuous subcultivation. Cultivation of hairy roots on solid medium was favorable for biomass production, while liquid culture was beneficial for decussatin and decussatin-1-O-primeveroside accumulation. Cytokinins kinetin or N6-benzyladenine promoted somatic embryo maturation and germination as well as multiplication of obtained plantlets, while active charcoal reduced hyperhydricity. Plants regenerated from selected hairy root line 9 comprised two TL-DNA inserts, as confirmed by Southern blot analysis. They accumulated at least 2.5-fold more decussatin then nontransformed plants. Therefore, these plants could be valuable material to create xanthone high-yielding cultivars of G. utriculosa.",
journal = "Industrial Crops and Products",
title = "Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants",
volume = "130",
doi = "10.1016/J.INDCROP.2018.12.066",
pages = "216-229"
}

Vinterhalter, B., Savić, J., Zdravković-Korać, S., Banjac, N., Vinterhalter, D.,& Krstić Milošević, D.. (2019). Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants. in Industrial Crops and Products, 130, 216-229.
https://doi.org/10.1016/J.INDCROP.2018.12.066

Vinterhalter B, Savić J, Zdravković-Korać S, Banjac N, Vinterhalter D, Krstić Milošević D. Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants. in Industrial Crops and Products. 2019;130:216-229.
doi:10.1016/J.INDCROP.2018.12.066 .

Vinterhalter, Branka, Savić, Jelena, Zdravković-Korać, Snežana, Banjac, Nevena, Vinterhalter, Dragan, Krstić Milošević, Dijana, "Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants" in Industrial Crops and Products, 130 (2019):216-229,
https://doi.org/10.1016/J.INDCROP.2018.12.066 . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
AU  - Zdravković-Korać, Snežana
AU  - Banjac, Nevena
AU  - Vinterhalter, Dragan
AU  - Krstić Milošević, Dijana
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0926669018311282?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3234
AB  - Production of innovative drugs from natural products in controlled conditions plays an important role in modern pharmacology in order to tackle global health challenges. The potential of Gentiana utriculosa hairy roots, obtained by transformation with Agrobacterium rhizogenes A4M70GUS, as well as that of the shoots regenerated from them via somatic embryogenesis, for xanthone production was investigated. Gentiana utriculosa was shown to be a new source of xanthones, medicinal raw materials for different pharmaceutical applications, among which decussatin has been recently recognized as a prospective hepatoprotective and antiulcer compound. Decussatin and decussatin-1-O-primeveroside were detected in both hairy roots and transgenic shoots, while mangiferin, present in nontransformed plants, was not detected. Quantitative HPLC analysis revealed up to 4.5-fold higher decussatin production in hairy roots line 9 compared with the plants from the nature, and this clone was selected. It showed stable growth after more than two years of continuous subcultivation. Cultivation of hairy roots on solid medium was favorable for biomass production, while liquid culture was beneficial for decussatin and decussatin-1-O-primeveroside accumulation. Cytokinins kinetin or N6-benzyladenine promoted somatic embryo maturation and germination as well as multiplication of obtained plantlets, while active charcoal reduced hyperhydricity. Plants regenerated from selected hairy root line 9 comprised two TL-DNA inserts, as confirmed by Southern blot analysis. They accumulated at least 2.5-fold more decussatin then nontransformed plants. Therefore, these plants could be valuable material to create xanthone high-yielding cultivars of G. utriculosa.
T2  - Industrial Crops and Products
T1  - Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants
DO  - 10.1016/J.INDCROP.2018.12.066
ER  - 

@article{
author = "Vinterhalter, Branka and Savić, Jelena and Zdravković-Korać, Snežana and Banjac, Nevena and Vinterhalter, Dragan and Krstić Milošević, Dijana",
year = "2018",
abstract = "Production of innovative drugs from natural products in controlled conditions plays an important role in modern pharmacology in order to tackle global health challenges. The potential of Gentiana utriculosa hairy roots, obtained by transformation with Agrobacterium rhizogenes A4M70GUS, as well as that of the shoots regenerated from them via somatic embryogenesis, for xanthone production was investigated. Gentiana utriculosa was shown to be a new source of xanthones, medicinal raw materials for different pharmaceutical applications, among which decussatin has been recently recognized as a prospective hepatoprotective and antiulcer compound. Decussatin and decussatin-1-O-primeveroside were detected in both hairy roots and transgenic shoots, while mangiferin, present in nontransformed plants, was not detected. Quantitative HPLC analysis revealed up to 4.5-fold higher decussatin production in hairy roots line 9 compared with the plants from the nature, and this clone was selected. It showed stable growth after more than two years of continuous subcultivation. Cultivation of hairy roots on solid medium was favorable for biomass production, while liquid culture was beneficial for decussatin and decussatin-1-O-primeveroside accumulation. Cytokinins kinetin or N6-benzyladenine promoted somatic embryo maturation and germination as well as multiplication of obtained plantlets, while active charcoal reduced hyperhydricity. Plants regenerated from selected hairy root line 9 comprised two TL-DNA inserts, as confirmed by Southern blot analysis. They accumulated at least 2.5-fold more decussatin then nontransformed plants. Therefore, these plants could be valuable material to create xanthone high-yielding cultivars of G. utriculosa.",
journal = "Industrial Crops and Products",
title = "Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants",
doi = "10.1016/J.INDCROP.2018.12.066"
}

Vinterhalter, B., Savić, J., Zdravković-Korać, S., Banjac, N., Vinterhalter, D.,& Krstić Milošević, D.. (2018). Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants. in Industrial Crops and Products.
https://doi.org/10.1016/J.INDCROP.2018.12.066

Vinterhalter B, Savić J, Zdravković-Korać S, Banjac N, Vinterhalter D, Krstić Milošević D. Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants. in Industrial Crops and Products. 2018;.
doi:10.1016/J.INDCROP.2018.12.066 .

Vinterhalter, Branka, Savić, Jelena, Zdravković-Korać, Snežana, Banjac, Nevena, Vinterhalter, Dragan, Krstić Milošević, Dijana, "Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants" in Industrial Crops and Products (2018),
https://doi.org/10.1016/J.INDCROP.2018.12.066 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Savić, Jelena
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Senćanski, Jelena
AU  - Simonović, Mladen
AU  - Radović, Svetlana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2018
UR  - http://link.springer.com/10.1007/s11738-018-2677-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3057
AB  - This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 × 6H2O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.
T2  - Acta Physiologiae Plantarum
T1  - Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro
IS  - 6
VL  - 40
DO  - 10.1007/s11738-018-2677-9
SP  - 101
ER  - 

@article{
author = "Stanisavljević, Nemanja and Savić, Jelena and Jovanović, Živko and Miljuš-Đukić, Jovanka and Senćanski, Jelena and Simonović, Mladen and Radović, Svetlana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2018",
abstract = "This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 × 6H2O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.",
journal = "Acta Physiologiae Plantarum",
title = "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro",
number = "6",
volume = "40",
doi = "10.1007/s11738-018-2677-9",
pages = "101"
}

Stanisavljević, N., Savić, J., Jovanović, Ž., Miljuš-Đukić, J., Senćanski, J., Simonović, M., Radović, S., Vinterhalter, D.,& Vinterhalter, B.. (2018). Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum, 40(6), 101.
https://doi.org/10.1007/s11738-018-2677-9

Stanisavljević N, Savić J, Jovanović Ž, Miljuš-Đukić J, Senćanski J, Simonović M, Radović S, Vinterhalter D, Vinterhalter B. Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum. 2018;40(6):101.
doi:10.1007/s11738-018-2677-9 .

Stanisavljević, Nemanja, Savić, Jelena, Jovanović, Živko, Miljuš-Đukić, Jovanka, Senćanski, Jelena, Simonović, Mladen, Radović, Svetlana, Vinterhalter, Dragan, Vinterhalter, Branka, "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro" in Acta Physiologiae Plantarum, 40, no. 6 (2018):101,
https://doi.org/10.1007/s11738-018-2677-9 . .

TY  - JOUR
AU  - Tusevski, Oliver
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Vinterhalter, Dragan
AU  - Zdravković-Korać, Snežana
AU  - Petreska Stanoeva, Jasmina
AU  - Stefova, Marina
AU  - Gadzovska Simic, Sonja
PY  - 2017
UR  - http://link.springer.com/10.1007/s11240-016-1136-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2688
AB  - Three hairy root clones of Hypericum perforatum (HR 2, HR 15 and HR 27) transformed with Agrobacterium rhizogenes A4M70GUS and their corresponding regenerated shoot culture clones (HRRS) were compared for differences in growth, production of phenolic compounds, antioxidant and antimicrobial activities. Transgenic clones were selected on the basis of morphological evaluation, genetic and molecular analyses. The clone HR 2 had the highest biomass accumulation, while HR 27 showed the highest shoot regeneration potential. The total phenolics and flavan-3-ols were enhanced in all tested transgenic cultures, while total flavonoids and hypericins were augmented in HRRS clones compared to non-transformed shoots. The HRRS clones produced substantial amounts of chlorogenic acid and 3-p-coumaroylquinic acid. Regarding the flavonoids, they produced significant contents of luteolin hexoside (HRRS 2), quercitrin and quercetin (HRRS 15) and isoquercetin (HRRS 27), while HR 2 and 15 accumulated 4-O-methylkaempferol-O-hexoside and quercetin 6-C-glucoside, respectively. The HR 15 was promising for the production of catechin and procyanidin derivatives and together with its HRRS clone exhibited a high potential for hyperforin and adhyperforin production. All identified naphtodianthrones were confirmed in HRRS 2 and 15 clones. Among xanthones, mangiferin was found as the major compound in HRRS, while trihydroxy-1-metoxy-C-prenyl xanthone was dominant in HR clones. Antimicrobial activity of transgenic cultures revealed that HRRS 15 strongly inhibited the growth of Bacillus cereus, Micrococcus flavus, Pseudomonas aeruginosa and Escherichia coli. Altogether, H. perforatum HR and HRRS cultures could be proposed as promising experimental systems for enhanced production of phenolic compounds with antioxidant and antibacterial properties.
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.
IS  - 3
VL  - 128
DO  - 10.1007/s11240-016-1136-9
SP  - 589
EP  - 605
ER  - 

@article{
author = "Tusevski, Oliver and Vinterhalter, Branka and Krstić Milošević, Dijana and Soković, Marina and Ćirić, Ana and Vinterhalter, Dragan and Zdravković-Korać, Snežana and Petreska Stanoeva, Jasmina and Stefova, Marina and Gadzovska Simic, Sonja",
year = "2017",
abstract = "Three hairy root clones of Hypericum perforatum (HR 2, HR 15 and HR 27) transformed with Agrobacterium rhizogenes A4M70GUS and their corresponding regenerated shoot culture clones (HRRS) were compared for differences in growth, production of phenolic compounds, antioxidant and antimicrobial activities. Transgenic clones were selected on the basis of morphological evaluation, genetic and molecular analyses. The clone HR 2 had the highest biomass accumulation, while HR 27 showed the highest shoot regeneration potential. The total phenolics and flavan-3-ols were enhanced in all tested transgenic cultures, while total flavonoids and hypericins were augmented in HRRS clones compared to non-transformed shoots. The HRRS clones produced substantial amounts of chlorogenic acid and 3-p-coumaroylquinic acid. Regarding the flavonoids, they produced significant contents of luteolin hexoside (HRRS 2), quercitrin and quercetin (HRRS 15) and isoquercetin (HRRS 27), while HR 2 and 15 accumulated 4-O-methylkaempferol-O-hexoside and quercetin 6-C-glucoside, respectively. The HR 15 was promising for the production of catechin and procyanidin derivatives and together with its HRRS clone exhibited a high potential for hyperforin and adhyperforin production. All identified naphtodianthrones were confirmed in HRRS 2 and 15 clones. Among xanthones, mangiferin was found as the major compound in HRRS, while trihydroxy-1-metoxy-C-prenyl xanthone was dominant in HR clones. Antimicrobial activity of transgenic cultures revealed that HRRS 15 strongly inhibited the growth of Bacillus cereus, Micrococcus flavus, Pseudomonas aeruginosa and Escherichia coli. Altogether, H. perforatum HR and HRRS cultures could be proposed as promising experimental systems for enhanced production of phenolic compounds with antioxidant and antibacterial properties.",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.",
number = "3",
volume = "128",
doi = "10.1007/s11240-016-1136-9",
pages = "589-605"
}

Tusevski, O., Vinterhalter, B., Krstić Milošević, D., Soković, M., Ćirić, A., Vinterhalter, D., Zdravković-Korać, S., Petreska Stanoeva, J., Stefova, M.,& Gadzovska Simic, S.. (2017). Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.. in Plant Cell, Tissue and Organ Culture (PCTOC), 128(3), 589-605.
https://doi.org/10.1007/s11240-016-1136-9

Tusevski O, Vinterhalter B, Krstić Milošević D, Soković M, Ćirić A, Vinterhalter D, Zdravković-Korać S, Petreska Stanoeva J, Stefova M, Gadzovska Simic S. Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.. in Plant Cell, Tissue and Organ Culture (PCTOC). 2017;128(3):589-605.
doi:10.1007/s11240-016-1136-9 .

Tusevski, Oliver, Vinterhalter, Branka, Krstić Milošević, Dijana, Soković, Marina, Ćirić, Ana, Vinterhalter, Dragan, Zdravković-Korać, Snežana, Petreska Stanoeva, Jasmina, Stefova, Marina, Gadzovska Simic, Sonja, "Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L." in Plant Cell, Tissue and Organ Culture (PCTOC), 128, no. 3 (2017):589-605,
https://doi.org/10.1007/s11240-016-1136-9 . .

TY  - JOUR
AU  - Krstić Milošević, Dijana
AU  - Janković, Teodora
AU  - Uzelac, Branka
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2017
UR  - https://link.springer.com/article/10.1007/s11240-017-1252-1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2773
AB  - The effect of abiotic (salicylic acid, jasmonic acid and methyl jasmonate) and biotic (chitosan and yeast extract) elicitors on the growth and xanthone accumulation in two hairy root clones of Gentiana dinarica Beck. was studied. The obtained results showed that clone 3 was more responsive to elicitor treatment than clone D. The production of dominant xanthone norswertianin-1-O-primeveroside was not significantly affected by either of the abiotic elicitor tested but was stimulated with chitosan treatment. The highest concentrations of all elicitors strongly increased the content of xanthone aglycone norswertianin, but simultaneously reducing the production of its glycoside norswertianin-1-O-primeveroside. The most efficient in enhancing norswertianin production was a 7-day treatment with salicylic acid (200 μM) and chitosan (50 mg l− 1), which yielded a 7.7- and a 24-fold increase in norswertianin content, respectively. In addition, treatment with biotic elicitors caused the occurrence of new xanthone compounds that were not detected in other samples. Free radical scavenging activity of xanthones was carried out by DPPH assay, and norswertianin showed the strongest activity.
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica
VL  - 130
DO  - 10.1007/s11240-017-1252-1
SP  - 631
EP  - 640
ER  - 

@article{
author = "Krstić Milošević, Dijana and Janković, Teodora and Uzelac, Branka and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2017",
abstract = "The effect of abiotic (salicylic acid, jasmonic acid and methyl jasmonate) and biotic (chitosan and yeast extract) elicitors on the growth and xanthone accumulation in two hairy root clones of Gentiana dinarica Beck. was studied. The obtained results showed that clone 3 was more responsive to elicitor treatment than clone D. The production of dominant xanthone norswertianin-1-O-primeveroside was not significantly affected by either of the abiotic elicitor tested but was stimulated with chitosan treatment. The highest concentrations of all elicitors strongly increased the content of xanthone aglycone norswertianin, but simultaneously reducing the production of its glycoside norswertianin-1-O-primeveroside. The most efficient in enhancing norswertianin production was a 7-day treatment with salicylic acid (200 μM) and chitosan (50 mg l− 1), which yielded a 7.7- and a 24-fold increase in norswertianin content, respectively. In addition, treatment with biotic elicitors caused the occurrence of new xanthone compounds that were not detected in other samples. Free radical scavenging activity of xanthones was carried out by DPPH assay, and norswertianin showed the strongest activity.",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica",
volume = "130",
doi = "10.1007/s11240-017-1252-1",
pages = "631-640"
}

Krstić Milošević, D., Janković, T., Uzelac, B., Vinterhalter, D.,& Vinterhalter, B.. (2017). Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica. in Plant Cell, Tissue and Organ Culture (PCTOC), 130, 631-640.
https://doi.org/10.1007/s11240-017-1252-1

Krstić Milošević D, Janković T, Uzelac B, Vinterhalter D, Vinterhalter B. Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica. in Plant Cell, Tissue and Organ Culture (PCTOC). 2017;130:631-640.
doi:10.1007/s11240-017-1252-1 .

Krstić Milošević, Dijana, Janković, Teodora, Uzelac, Branka, Vinterhalter, Dragan, Vinterhalter, Branka, "Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica" in Plant Cell, Tissue and Organ Culture (PCTOC), 130 (2017):631-640,
https://doi.org/10.1007/s11240-017-1252-1 . .

TY  - JOUR
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2017
UR  - http://link.springer.com/10.1007/s11240-017-1235-2
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2758
AB  - Intact and wounded shoots of in vitro cultured potato were investigated in situ to determine how their phototropic (PT) bending capacity was affected by water availability and exogenous auxin supplementation. Wounding strongly decreased PT bending but it recovered upon supplementation of water, auxin or both. Intact shoots required neither water nor auxin, while shoot segments required both. Shoot cuttings required only water, while, like shoot segments, decapitated shoots had a strong requirement for auxin. Water supplementation was beneficial in all treatments and PT bending was not affected in cultures that were submerged in water for a short period. Sucrose and inorganic salts present in the medium strongly affected PT bending capacity of cultures, favouring combinations with lower concentrations of both. Sucrose alone strongly promoted PT bending up to a concentration of 5%. Osmotic shock induced by the addition of small volumes of highly concentrated carbohydrate solutions (sucrose, glucose or sorbitol) induced a rapid but transient decline in PT bending capacity. These results indicate that water availability is a major factor that affects PT bending in potato plantlets.
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Phototropic bending of intact and wounded potato shoots
IS  - 2
VL  - 130
DO  - 10.1007/s11240-017-1235-2
SP  - 393
EP  - 404
ER  - 

@article{
author = "Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2017",
abstract = "Intact and wounded shoots of in vitro cultured potato were investigated in situ to determine how their phototropic (PT) bending capacity was affected by water availability and exogenous auxin supplementation. Wounding strongly decreased PT bending but it recovered upon supplementation of water, auxin or both. Intact shoots required neither water nor auxin, while shoot segments required both. Shoot cuttings required only water, while, like shoot segments, decapitated shoots had a strong requirement for auxin. Water supplementation was beneficial in all treatments and PT bending was not affected in cultures that were submerged in water for a short period. Sucrose and inorganic salts present in the medium strongly affected PT bending capacity of cultures, favouring combinations with lower concentrations of both. Sucrose alone strongly promoted PT bending up to a concentration of 5%. Osmotic shock induced by the addition of small volumes of highly concentrated carbohydrate solutions (sucrose, glucose or sorbitol) induced a rapid but transient decline in PT bending capacity. These results indicate that water availability is a major factor that affects PT bending in potato plantlets.",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Phototropic bending of intact and wounded potato shoots",
number = "2",
volume = "130",
doi = "10.1007/s11240-017-1235-2",
pages = "393-404"
}

Vinterhalter, D.,& Vinterhalter, B.. (2017). Phototropic bending of intact and wounded potato shoots. in Plant Cell, Tissue and Organ Culture (PCTOC), 130(2), 393-404.
https://doi.org/10.1007/s11240-017-1235-2

Vinterhalter D, Vinterhalter B. Phototropic bending of intact and wounded potato shoots. in Plant Cell, Tissue and Organ Culture (PCTOC). 2017;130(2):393-404.
doi:10.1007/s11240-017-1235-2 .

Vinterhalter, Dragan, Vinterhalter, Branka, "Phototropic bending of intact and wounded potato shoots" in Plant Cell, Tissue and Organ Culture (PCTOC), 130, no. 2 (2017):393-404,
https://doi.org/10.1007/s11240-017-1235-2 . .

TY  - GEN
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Banjac, Nevena
AU  - Bohanec, Borut
AU  - Vinterhalter, Dragan
AU  - Savić, Jelena
PY  - 2016
UR  - http://link.springer.com/10.1007/s11738-016-2264-x
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84989910868&origin=SingleRecordEmailAlert&txGid=66B9A38F509F4540CF41E07DD21FB6C0.wsnAw8kcdt7IPYLO0V48gA:1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2984
AB  - The corresponding author of this paper, Jelena Savić, has acknowledged that she manipulated two bands in Fig. 5c. The original figure is given here. The modification does not alter the interpretation of the data in any way. The author sincerely regrets the mistake and would like to apologize to her co-authors, the editors and readers (Figure presented).
PB  - Polish Academy of Sciences
T2  - Acta Physiologiae Plantarum
T1  - Erratum to: Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots
IS  - 10
VL  - 38
DO  - 10.1007/s11738-016-2264-x
SP  - 253
ER  - 

@misc{
author = "Vinterhalter, Branka and Zdravković-Korać, Snežana and Banjac, Nevena and Bohanec, Borut and Vinterhalter, Dragan and Savić, Jelena",
year = "2016",
abstract = "The corresponding author of this paper, Jelena Savić, has acknowledged that she manipulated two bands in Fig. 5c. The original figure is given here. The modification does not alter the interpretation of the data in any way. The author sincerely regrets the mistake and would like to apologize to her co-authors, the editors and readers (Figure presented).",
publisher = "Polish Academy of Sciences",
journal = "Acta Physiologiae Plantarum",
title = "Erratum to: Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots",
number = "10",
volume = "38",
doi = "10.1007/s11738-016-2264-x",
pages = "253"
}

Vinterhalter, B., Zdravković-Korać, S., Banjac, N., Bohanec, B., Vinterhalter, D.,& Savić, J.. (2016). Erratum to: Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots. in Acta Physiologiae Plantarum
Polish Academy of Sciences., 38(10), 253.
https://doi.org/10.1007/s11738-016-2264-x

Vinterhalter B, Zdravković-Korać S, Banjac N, Bohanec B, Vinterhalter D, Savić J. Erratum to: Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots. in Acta Physiologiae Plantarum. 2016;38(10):253.
doi:10.1007/s11738-016-2264-x .

Vinterhalter, Branka, Zdravković-Korać, Snežana, Banjac, Nevena, Bohanec, Borut, Vinterhalter, Dragan, Savić, Jelena, "Erratum to: Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots" in Acta Physiologiae Plantarum, 38, no. 10 (2016):253,
https://doi.org/10.1007/s11738-016-2264-x . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Banjac, Nevena
AU  - Vinterhalter, Dragan
AU  - Uzelac, Branka
AU  - Krstić Milošević, Dijana
PY  - 2016
UR  - https://www.degruyter.com/view/j/biolog.2016.71.issue-2/biolog-2016-0020/biolog-2016-0020.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2985
AB  - Study describes protocols for in vitro propagation of Gentiana utriculosa L. via axillary shoot multiplication and indirect somatic embryogenesis. Shoot cultures were established from seedling epicotyl explants cultured on MS medium supplemented with 0.25 mg L-1 BA and 0.1 mg L-1 IAA. Medium containing 2% sucrose and 0.2 mg L-1 BA improved multiple shoot production, providing 2.3 shoots per explant. The highest rooting (29.6%) was obtained on medium with 1/2 MS mineral salts and 0.5 mg L-1 NAA. Somatic embryogenesis was induced using different explants, including immature seeds as well as leaves and roots from shoot cultures. Following auxin treatment with either 1.0 mg L-1 2,4-D (immature seeds and leaves) or 0.1 mg L-1 NAA (roots), explants produced embryogenic calli which upon transfer to plant growth regulator-free medium allowed embryo conversion into plantlets. The best embryogenic response (82%) was obtained in calli derived from leaves cultured with their abaxial surface in contact with medium, whereas the highest embryo conversion rate (68%) was recorded for calli induced on immature seed explants. Histological analysis in all explant types revealed development of proembryogenic cell complexes at callus periphery, giving rise to somatic embryos. The presence of embryos at various stages of development indicated asynchronous somatic embryogenesis in G. utriculosa. Derooted embryo-derived plantlets placed on medium with 0.2 mg L-1 BA multiplied further as shoot cultures.
PB  - De Gruyter Open Ltd
T2  - Biologia
T1  - Somatic embryogenesis and in vitro shoot propagation of Gentiana utriculosa
IS  - 2
VL  - 71
DO  - 10.1515/biolog-2016-0020
SP  - 139
EP  - 148
ER  - 

@article{
author = "Vinterhalter, Branka and Banjac, Nevena and Vinterhalter, Dragan and Uzelac, Branka and Krstić Milošević, Dijana",
year = "2016",
abstract = "Study describes protocols for in vitro propagation of Gentiana utriculosa L. via axillary shoot multiplication and indirect somatic embryogenesis. Shoot cultures were established from seedling epicotyl explants cultured on MS medium supplemented with 0.25 mg L-1 BA and 0.1 mg L-1 IAA. Medium containing 2% sucrose and 0.2 mg L-1 BA improved multiple shoot production, providing 2.3 shoots per explant. The highest rooting (29.6%) was obtained on medium with 1/2 MS mineral salts and 0.5 mg L-1 NAA. Somatic embryogenesis was induced using different explants, including immature seeds as well as leaves and roots from shoot cultures. Following auxin treatment with either 1.0 mg L-1 2,4-D (immature seeds and leaves) or 0.1 mg L-1 NAA (roots), explants produced embryogenic calli which upon transfer to plant growth regulator-free medium allowed embryo conversion into plantlets. The best embryogenic response (82%) was obtained in calli derived from leaves cultured with their abaxial surface in contact with medium, whereas the highest embryo conversion rate (68%) was recorded for calli induced on immature seed explants. Histological analysis in all explant types revealed development of proembryogenic cell complexes at callus periphery, giving rise to somatic embryos. The presence of embryos at various stages of development indicated asynchronous somatic embryogenesis in G. utriculosa. Derooted embryo-derived plantlets placed on medium with 0.2 mg L-1 BA multiplied further as shoot cultures.",
publisher = "De Gruyter Open Ltd",
journal = "Biologia",
title = "Somatic embryogenesis and in vitro shoot propagation of Gentiana utriculosa",
number = "2",
volume = "71",
doi = "10.1515/biolog-2016-0020",
pages = "139-148"
}

Vinterhalter, B., Banjac, N., Vinterhalter, D., Uzelac, B.,& Krstić Milošević, D.. (2016). Somatic embryogenesis and in vitro shoot propagation of Gentiana utriculosa. in Biologia
De Gruyter Open Ltd., 71(2), 139-148.
https://doi.org/10.1515/biolog-2016-0020

Vinterhalter B, Banjac N, Vinterhalter D, Uzelac B, Krstić Milošević D. Somatic embryogenesis and in vitro shoot propagation of Gentiana utriculosa. in Biologia. 2016;71(2):139-148.
doi:10.1515/biolog-2016-0020 .

Vinterhalter, Branka, Banjac, Nevena, Vinterhalter, Dragan, Uzelac, Branka, Krstić Milošević, Dijana, "Somatic embryogenesis and in vitro shoot propagation of Gentiana utriculosa" in Biologia, 71, no. 2 (2016):139-148,
https://doi.org/10.1515/biolog-2016-0020 . .

TY  - JOUR
AU  - Vinterhalter, Dragan
AU  - Savić, Jelena
AU  - Stanišić, Mariana
AU  - Jovanović, Ž
AU  - Vinterhalter, Branka
PY  - 2016
UR  - https://link.springer.com/article/10.1007%2Fs10265-016-0821-4#Notes
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2983
AB  - Phototropic (PT) and gravitropic (GT) bending are the two major tropic movements that determine the spatial position of potato shoots. We studied PT bending of potato plantlets grown under long-day photoperiods in several prearranged position setups providing different interactions with the GT response. Starting with the standard PT stimulation setup composed of unilateral irradiation of vertically positioned shoots, experiments were also done in antagonistic and synergistic setups and in treatments with horizontal displacement of the light source. In the standard setup, PT bending suppressed the GT bending, which could occur only if the PT stimulation was cancelled. The antagonistic position, with phototropism and gravitropism attempting to bend shoots in opposite directions, showed phototropism and gravitropism as independent bending events with the outcome varying throughout the day reflecting diurnal changes in the competence of individual tropic components. Whilst gravitropism was constant, phototropism had a marked daily fluctuation of its magnitude with a prominent morning maximum starting an hour after the dawn in the growth room and lasting for the next 6 h. When phototropism and gravitropism were aligned in a synergistic position, stimulating shoot bending in the same direction, there was little quantitative addition of their individual effects. The long period of morning PT bending maximum enabled multiple PT bending events to be conducted in succession, each one preceded by a separate lag phase. Studies of secondary PT events showed that potato plantlets can follow and adjust their shoot position in response to both vertical and horizontal movements of a light source. PT bending was reversible, since the 180° horizontal change of a blue light (BL) source position resulted in reversal of bending direction after a 20-min-long lag phase.
PB  - Springer-Verlag Tokyo
T2  - Journal of plant research
T1  - Interaction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots
DO  - 10.1007/s10265-016-0821-4
SP  - 1
EP  - 12
ER  - 

@article{
author = "Vinterhalter, Dragan and Savić, Jelena and Stanišić, Mariana and Jovanović, Ž and Vinterhalter, Branka",
year = "2016",
abstract = "Phototropic (PT) and gravitropic (GT) bending are the two major tropic movements that determine the spatial position of potato shoots. We studied PT bending of potato plantlets grown under long-day photoperiods in several prearranged position setups providing different interactions with the GT response. Starting with the standard PT stimulation setup composed of unilateral irradiation of vertically positioned shoots, experiments were also done in antagonistic and synergistic setups and in treatments with horizontal displacement of the light source. In the standard setup, PT bending suppressed the GT bending, which could occur only if the PT stimulation was cancelled. The antagonistic position, with phototropism and gravitropism attempting to bend shoots in opposite directions, showed phototropism and gravitropism as independent bending events with the outcome varying throughout the day reflecting diurnal changes in the competence of individual tropic components. Whilst gravitropism was constant, phototropism had a marked daily fluctuation of its magnitude with a prominent morning maximum starting an hour after the dawn in the growth room and lasting for the next 6 h. When phototropism and gravitropism were aligned in a synergistic position, stimulating shoot bending in the same direction, there was little quantitative addition of their individual effects. The long period of morning PT bending maximum enabled multiple PT bending events to be conducted in succession, each one preceded by a separate lag phase. Studies of secondary PT events showed that potato plantlets can follow and adjust their shoot position in response to both vertical and horizontal movements of a light source. PT bending was reversible, since the 180° horizontal change of a blue light (BL) source position resulted in reversal of bending direction after a 20-min-long lag phase.",
publisher = "Springer-Verlag Tokyo",
journal = "Journal of plant research",
title = "Interaction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots",
doi = "10.1007/s10265-016-0821-4",
pages = "1-12"
}

Vinterhalter, D., Savić, J., Stanišić, M., Jovanović, Ž.,& Vinterhalter, B.. (2016). Interaction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots. in Journal of plant research
Springer-Verlag Tokyo., 1-12.
https://doi.org/10.1007/s10265-016-0821-4

Vinterhalter D, Savić J, Stanišić M, Jovanović Ž, Vinterhalter B. Interaction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots. in Journal of plant research. 2016;:1-12.
doi:10.1007/s10265-016-0821-4 .

Vinterhalter, Dragan, Savić, Jelena, Stanišić, Mariana, Jovanović, Ž, Vinterhalter, Branka, "Interaction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots" in Journal of plant research (2016):1-12,
https://doi.org/10.1007/s10265-016-0821-4 . .

TY  - JOUR
AU  - Trajković, Milena
AU  - Vinterhalter, Branka
AU  - Subotić, Angelina
AU  - Vinterhalter, Dragan
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6412
AB  - The regenerative potential of in vitro-produced bulblets was investigated in three commercial Oriental lily cultivars (‘Aubade’, ‘Belcanto’ and ‘Solaia’) in relation to two synthetic cytokinins  BA and TDZ and picloram as representative of auxins. Single bulblet leaflets were excised and cultured on MS medium supplemented with either 6-benzyladenine (BA 0-2.0 mg/l), thidiazurone (TDZ 0-2.0 mg/l) or picloram (PIC 0-3.0 mg/l). In all three cultivars and medium combinations explants after 5 weeks regenerated somatic embryos, bulblets and plantlets. While bulblet production was balanced, plantlet and somatic embryogenesis (SE) production were complementary with pronounced SE production at higher plant growth regulator concentrations and plantlet production at lower concentrations. Picloram had a sharp regeneration demarcation with low plantlet production above 0.5mg/l. BA and TDZ produced SE at all concentrations including hormone-free controls. On media with TDZ and BA there was a gradual change from bulblet regeneration at lower to somatic embryogenesis at higher concentrations. For all three cultivars, details of the regeneration process were studied by histological techniques in TDZ-supplemented medium, showing early stage SE regeneration in all samples. Mature, elongated SE stages were missing, indicating early transition of SE into bulblets. The optimal propagation conditions were elaborated for all three lily cultivars.
AB  - Regenerativni potencijal in vitro razmnoženih lukovica istražen je kod tri komercijalne sorte Orijental ljiljana (‘Aubade’, ‘Belcanto’ and ‘Solaia’) u odnosu na dva sintetička citokinina BAP i  TDZ kao i na pikloram regulator rastenja sa auksinskom aktivnošću. Listići sa in vitro lukovica su izolovani i kultivisani na MS podlogama sa 6-benzil aminopurinom (BAP 0-2.0 mg/l), thidiazuronom (TDZ 0-2.0 mg/l) ili pikloramom (PIC 0-3.0 mg/l). Kod sve tri sorte i u svim kombinacijama podloga eksplantati su nakon 5 nedelja regenerisali somatske embrione, lukovičice ili biljke. Dok je produkcija lukovičica bila izbalansirana produkcija biljaka i produkcija ranih somatskih embriona bile su komplementarne sa izrazitom produkcijom SE na višim koncentracijama regulatora rastenja i produkcijom biljaka na nižim koncentracijama. Pikloram je pokazivao jasnu regeneracionu demarkaciju sa niskom produkcijom biljaka na podlogama sa ili iznad 0.5 mg/l. BAP i TDZ su produkovali somatske embrione na svim koncentracijama regulatora rastenja uključujući i podloge bez regulatora rastenja. Podloge sa  TDZ i BAP pokazivale su postepenu promenu od regeneracije lukovica na nižim prema SE na višim koncentracijama. Kod sva tri kultivara proces regeneracije praćen je histološki na podlogama sa TDZ i pokazano je prisustvo direktne SE. Stariji, izduženi stupnjevi SE su bili odsutni što ukazuje na ranu tranziciju somatskih embriona ljiljana u lukovičice. Optimalni uslovi za razmnožavanje razradjeni su i prikazani za sva tri sorte ljiljana.
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars
T1  - Različiti regenerativni potencijal kultivara orjentalnog ljiljana (Lilium sp.)
IS  - 2
VL  - 39
SP  - 159
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6412
ER  - 

@article{
author = "Trajković, Milena and Vinterhalter, Branka and Subotić, Angelina and Vinterhalter, Dragan",
year = "2015",
abstract = "The regenerative potential of in vitro-produced bulblets was investigated in three commercial Oriental lily cultivars (‘Aubade’, ‘Belcanto’ and ‘Solaia’) in relation to two synthetic cytokinins  BA and TDZ and picloram as representative of auxins. Single bulblet leaflets were excised and cultured on MS medium supplemented with either 6-benzyladenine (BA 0-2.0 mg/l), thidiazurone (TDZ 0-2.0 mg/l) or picloram (PIC 0-3.0 mg/l). In all three cultivars and medium combinations explants after 5 weeks regenerated somatic embryos, bulblets and plantlets. While bulblet production was balanced, plantlet and somatic embryogenesis (SE) production were complementary with pronounced SE production at higher plant growth regulator concentrations and plantlet production at lower concentrations. Picloram had a sharp regeneration demarcation with low plantlet production above 0.5mg/l. BA and TDZ produced SE at all concentrations including hormone-free controls. On media with TDZ and BA there was a gradual change from bulblet regeneration at lower to somatic embryogenesis at higher concentrations. For all three cultivars, details of the regeneration process were studied by histological techniques in TDZ-supplemented medium, showing early stage SE regeneration in all samples. Mature, elongated SE stages were missing, indicating early transition of SE into bulblets. The optimal propagation conditions were elaborated for all three lily cultivars., Regenerativni potencijal in vitro razmnoženih lukovica istražen je kod tri komercijalne sorte Orijental ljiljana (‘Aubade’, ‘Belcanto’ and ‘Solaia’) u odnosu na dva sintetička citokinina BAP i  TDZ kao i na pikloram regulator rastenja sa auksinskom aktivnošću. Listići sa in vitro lukovica su izolovani i kultivisani na MS podlogama sa 6-benzil aminopurinom (BAP 0-2.0 mg/l), thidiazuronom (TDZ 0-2.0 mg/l) ili pikloramom (PIC 0-3.0 mg/l). Kod sve tri sorte i u svim kombinacijama podloga eksplantati su nakon 5 nedelja regenerisali somatske embrione, lukovičice ili biljke. Dok je produkcija lukovičica bila izbalansirana produkcija biljaka i produkcija ranih somatskih embriona bile su komplementarne sa izrazitom produkcijom SE na višim koncentracijama regulatora rastenja i produkcijom biljaka na nižim koncentracijama. Pikloram je pokazivao jasnu regeneracionu demarkaciju sa niskom produkcijom biljaka na podlogama sa ili iznad 0.5 mg/l. BAP i TDZ su produkovali somatske embrione na svim koncentracijama regulatora rastenja uključujući i podloge bez regulatora rastenja. Podloge sa  TDZ i BAP pokazivale su postepenu promenu od regeneracije lukovica na nižim prema SE na višim koncentracijama. Kod sva tri kultivara proces regeneracije praćen je histološki na podlogama sa TDZ i pokazano je prisustvo direktne SE. Stariji, izduženi stupnjevi SE su bili odsutni što ukazuje na ranu tranziciju somatskih embriona ljiljana u lukovičice. Optimalni uslovi za razmnožavanje razradjeni su i prikazani za sva tri sorte ljiljana.",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars, Različiti regenerativni potencijal kultivara orjentalnog ljiljana (Lilium sp.)",
number = "2",
volume = "39",
pages = "159-167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6412"
}

Trajković, M., Vinterhalter, B., Subotić, A.,& Vinterhalter, D.. (2015). Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 39(2), 159-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6412

Trajković M, Vinterhalter B, Subotić A, Vinterhalter D. Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars. in Botanica Serbica. 2015;39(2):159-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6412 .

Trajković, Milena, Vinterhalter, Branka, Subotić, Angelina, Vinterhalter, Dragan, "Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars" in Botanica Serbica, 39, no. 2 (2015):159-167,
https://hdl.handle.net/21.15107/rcub_ibiss_6412 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Banjac, Nevena
AU  - Bohanec, Borut
AU  - Vinterhalter, Dragan
AU  - Savić, Jelena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2006
AB  - The aim of the present study was to investigate the effect of sucrose on
   shoot regeneration potential in Hypericum perforatum L. roots obtained
   by Agrobacterium rhizogenes transformation. The morphological evaluation
   of transgenic roots grown on media supplemented with sucrose (0.5, 1, 2,
   4, 6 and 8 \%) indicated that both genotype and sucrose concentration
   significantly affected root elongation and branching, as well as shoot
   regeneration. For two of five analyzed clones, lower sucrose
   concentrations (up to 2 \%) led to intensive shoot regeneration, while
   the other three clones intensified shoot development only at elevated
   sucrose concentrations (4 \%). For all clones, concentrations above 4 \%
   had a deleterious effect on both root and shoot development. Genetic
   characterization of regenerated shoots revealed that all tested clones
   were diploid with an average of 0.670 +/- 0.002 pg of DNA per nucleus,
   with no significant differences between transgenic and non-transformed
   plants and, according to PCR, with integrated A. rhizogenes rolA, -B, -C
   and -D genes. Real-time RT-PCR confirmed the expression of rolA, -B and
   -C, while expression of the rolD gene was not detected. Differences were
   detected in the absolute amounts of transcripts between analyzed clones,
   with the highest levels of expression for all three analyzed rol genes
   in a clone previously defined as having high root differentiation and
   less effective shoot regeneration potential. The observed variations in
   morphogenesis potential could be attributed to different levels of
   expression of integrated rolA, -B and -Cgenes; while sucrose
   additionally pointed out these trends.
T2  - Acta Physiologiae Plantarum
T1  - Effect of sucrose on shoot regeneration in Agrobacterium transformed
 Hypericum perforatum L. roots
VL  - 37
DO  - 10.1007/s11738-015-1785-z
SP  - 37
ER  - 

@article{
author = "Vinterhalter, Branka and Zdravković-Korać, Snežana and Banjac, Nevena and Bohanec, Borut and Vinterhalter, Dragan and Savić, Jelena",
year = "2015",
abstract = "The aim of the present study was to investigate the effect of sucrose on
   shoot regeneration potential in Hypericum perforatum L. roots obtained
   by Agrobacterium rhizogenes transformation. The morphological evaluation
   of transgenic roots grown on media supplemented with sucrose (0.5, 1, 2,
   4, 6 and 8 \%) indicated that both genotype and sucrose concentration
   significantly affected root elongation and branching, as well as shoot
   regeneration. For two of five analyzed clones, lower sucrose
   concentrations (up to 2 \%) led to intensive shoot regeneration, while
   the other three clones intensified shoot development only at elevated
   sucrose concentrations (4 \%). For all clones, concentrations above 4 \%
   had a deleterious effect on both root and shoot development. Genetic
   characterization of regenerated shoots revealed that all tested clones
   were diploid with an average of 0.670 +/- 0.002 pg of DNA per nucleus,
   with no significant differences between transgenic and non-transformed
   plants and, according to PCR, with integrated A. rhizogenes rolA, -B, -C
   and -D genes. Real-time RT-PCR confirmed the expression of rolA, -B and
   -C, while expression of the rolD gene was not detected. Differences were
   detected in the absolute amounts of transcripts between analyzed clones,
   with the highest levels of expression for all three analyzed rol genes
   in a clone previously defined as having high root differentiation and
   less effective shoot regeneration potential. The observed variations in
   morphogenesis potential could be attributed to different levels of
   expression of integrated rolA, -B and -Cgenes; while sucrose
   additionally pointed out these trends.",
journal = "Acta Physiologiae Plantarum",
title = "Effect of sucrose on shoot regeneration in Agrobacterium transformed
 Hypericum perforatum L. roots",
volume = "37",
doi = "10.1007/s11738-015-1785-z",
pages = "37"
}

Vinterhalter, B., Zdravković-Korać, S., Banjac, N., Bohanec, B., Vinterhalter, D.,& Savić, J.. (2015). Effect of sucrose on shoot regeneration in Agrobacterium transformed
 Hypericum perforatum L. roots. in Acta Physiologiae Plantarum, 37, 37.
https://doi.org/10.1007/s11738-015-1785-z

Vinterhalter B, Zdravković-Korać S, Banjac N, Bohanec B, Vinterhalter D, Savić J. Effect of sucrose on shoot regeneration in Agrobacterium transformed
 Hypericum perforatum L. roots. in Acta Physiologiae Plantarum. 2015;37:37.
doi:10.1007/s11738-015-1785-z .

Vinterhalter, Branka, Zdravković-Korać, Snežana, Banjac, Nevena, Bohanec, Borut, Vinterhalter, Dragan, Savić, Jelena, "Effect of sucrose on shoot regeneration in Agrobacterium transformed
 Hypericum perforatum L. roots" in Acta Physiologiae Plantarum, 37 (2015):37,
https://doi.org/10.1007/s11738-015-1785-z . .

TY  - CHAP
AU  - Krstić Milošević, Dijana
AU  - Vinterhalter, Branka
AU  - Janković, Teodora
AU  - Vinterhalter, Dragan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2571
UR  - http://link.springer.com/chapter/10.1007%2F978-3-642-54102-5_4
AB  - In the central regions of the Balkan Peninsula, the genus Gentianella is represented by six species. Five of them G. albanica, G. austriaca, G. bulgarica, G. ciliata, and G. crispata were subject of phytochemical and in vitro studies. Two species, G. austriaca and G. bulgarica, were established as shoot cultures and procedures developed for their in vitro propagation. Difficulties with seed germination and precocious flowering affected the final propagation stages. Phytochemical analyses were performed on five species from nature and on cultured shoots. Plant material was rich in secondary metabolites with xanthones, flavone-C-glucosides, and secoiridoids as the principal constituents. The xanthone aglycones, demethy- bellidifolin, bellidifolin, corymbiferin, and their corresponding glucosides were dominant components in all the species investigated. The predominant substitution pattern of xanthones found in the genus Gentianella was 1,3,5,8 and to a lesser extent, 1,3,7,8. Lanceoside and veratriloside were new compounds found in European Gentianella species. In vitro material generally contained less secondary metabolites than plants from nature; the content of secondary products was influenced by the concentration of benzyladenine (BAP) in the culture medium.
PB  - Springer Berlin Heidelberg, Berlin, Heidelberg
T2  - The Gentianaceae - Volume 2: Biotechnology and Applications
T1  - Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula
DO  - 10.1007/978-3-642-54102-5
SP  - 93
EP  - 112
ER  - 

@inbook{
editor = "Rybczyński, Jan J., Davey, Michael R., Mikuła, Anna",
author = "Krstić Milošević, Dijana and Vinterhalter, Branka and Janković, Teodora and Vinterhalter, Dragan",
year = "2015",
abstract = "In the central regions of the Balkan Peninsula, the genus Gentianella is represented by six species. Five of them G. albanica, G. austriaca, G. bulgarica, G. ciliata, and G. crispata were subject of phytochemical and in vitro studies. Two species, G. austriaca and G. bulgarica, were established as shoot cultures and procedures developed for their in vitro propagation. Difficulties with seed germination and precocious flowering affected the final propagation stages. Phytochemical analyses were performed on five species from nature and on cultured shoots. Plant material was rich in secondary metabolites with xanthones, flavone-C-glucosides, and secoiridoids as the principal constituents. The xanthone aglycones, demethy- bellidifolin, bellidifolin, corymbiferin, and their corresponding glucosides were dominant components in all the species investigated. The predominant substitution pattern of xanthones found in the genus Gentianella was 1,3,5,8 and to a lesser extent, 1,3,7,8. Lanceoside and veratriloside were new compounds found in European Gentianella species. In vitro material generally contained less secondary metabolites than plants from nature; the content of secondary products was influenced by the concentration of benzyladenine (BAP) in the culture medium.",
publisher = "Springer Berlin Heidelberg, Berlin, Heidelberg",
journal = "The Gentianaceae - Volume 2: Biotechnology and Applications",
booktitle = "Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula",
doi = "10.1007/978-3-642-54102-5",
pages = "93-112"
}

Rybczyński, J. J., Davey, M. R., Mikuła, A., Krstić Milošević, D., Vinterhalter, B., Janković, T.,& Vinterhalter, D.. (2015). Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula. in The Gentianaceae - Volume 2: Biotechnology and Applications
Springer Berlin Heidelberg, Berlin, Heidelberg., 93-112.
https://doi.org/10.1007/978-3-642-54102-5

Rybczyński JJ, Davey MR, Mikuła A, Krstić Milošević D, Vinterhalter B, Janković T, Vinterhalter D. Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula. in The Gentianaceae - Volume 2: Biotechnology and Applications. 2015;:93-112.
doi:10.1007/978-3-642-54102-5 .

Rybczyński, Jan J., Davey, Michael R., Mikuła, Anna, Krstić Milošević, Dijana, Vinterhalter, Branka, Janković, Teodora, Vinterhalter, Dragan, "Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula" in The Gentianaceae - Volume 2: Biotechnology and Applications (2015):93-112,
https://doi.org/10.1007/978-3-642-54102-5 . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Jankovic, Teodora
AU  - Pljevljakusic, Dejan
AU  - Ninković, Slavica
AU  - Smigocki, Ann
AU  - Vinterhalter, Dragan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1952
AB  - The induction and establishment of hairy root cultures of Gentiana
   dinarica using two strains of Agrobacterium rhizogenes (A4M70GUS and
   15834/PI) is reported for the first time. Strain 15834/PI had higher
   induction rate of hairy roots (32.15 \%) than strain A4M70GUS (6.12 \%).
   Transgenic nature of the roots was confirmed by GUS assay and PCR
   analysis. Two clones per strain (A4M70GUS-D and -I, and 15834/PI-2 and
   -3) with marked differences in general morphology and growth rate were
   further studied. The methanol extracts of hairy root clones were
   analyzed for xanthones content using HPLC method. The effects of the
   type of carbohydrate source (sucrose, fructose or glucose) at different
   concentrations on the growth parameters (growth index, dry weight,
   fresh/dry weight ratio), phenolic and xanthone production, and free
   radical scavenging activity of the transgenic clones were evaluated.
   Statistical two level factorial design was used to define optimal
   conditions for growth and successful secondary metabolite production in
   G. dinarica hairy root clones. The results showed that clones A4M70GUS-D
   and 15834/PI-3 were the superior ones. These two clones had the highest
   dry weight on 116.8 mM sucrose, producing up to threefold higher amounts
   of total phenolics and norswertianin-1-O-primeveroside than other
   clones, untransformed roots and roots of wild-grown plants.
T2  - Plant Cell Tissue and Organ Culture
T1  - Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production
IS  - 3
VL  - 121
DO  - 10.1007/s11240-015-0737-z
SP  - 667
EP  - 679
ER  - 

@article{
author = "Vinterhalter, Branka and Krstić Milošević, Dijana and Jankovic, Teodora and Pljevljakusic, Dejan and Ninković, Slavica and Smigocki, Ann and Vinterhalter, Dragan",
year = "2015",
abstract = "The induction and establishment of hairy root cultures of Gentiana
   dinarica using two strains of Agrobacterium rhizogenes (A4M70GUS and
   15834/PI) is reported for the first time. Strain 15834/PI had higher
   induction rate of hairy roots (32.15 \%) than strain A4M70GUS (6.12 \%).
   Transgenic nature of the roots was confirmed by GUS assay and PCR
   analysis. Two clones per strain (A4M70GUS-D and -I, and 15834/PI-2 and
   -3) with marked differences in general morphology and growth rate were
   further studied. The methanol extracts of hairy root clones were
   analyzed for xanthones content using HPLC method. The effects of the
   type of carbohydrate source (sucrose, fructose or glucose) at different
   concentrations on the growth parameters (growth index, dry weight,
   fresh/dry weight ratio), phenolic and xanthone production, and free
   radical scavenging activity of the transgenic clones were evaluated.
   Statistical two level factorial design was used to define optimal
   conditions for growth and successful secondary metabolite production in
   G. dinarica hairy root clones. The results showed that clones A4M70GUS-D
   and 15834/PI-3 were the superior ones. These two clones had the highest
   dry weight on 116.8 mM sucrose, producing up to threefold higher amounts
   of total phenolics and norswertianin-1-O-primeveroside than other
   clones, untransformed roots and roots of wild-grown plants.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production",
number = "3",
volume = "121",
doi = "10.1007/s11240-015-0737-z",
pages = "667-679"
}

Vinterhalter, B., Krstić Milošević, D., Jankovic, T., Pljevljakusic, D., Ninković, S., Smigocki, A.,& Vinterhalter, D.. (2015). Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production. in Plant Cell Tissue and Organ Culture, 121(3), 667-679.
https://doi.org/10.1007/s11240-015-0737-z

Vinterhalter B, Krstić Milošević D, Jankovic T, Pljevljakusic D, Ninković S, Smigocki A, Vinterhalter D. Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production. in Plant Cell Tissue and Organ Culture. 2015;121(3):667-679.
doi:10.1007/s11240-015-0737-z .

Vinterhalter, Branka, Krstić Milošević, Dijana, Jankovic, Teodora, Pljevljakusic, Dejan, Ninković, Slavica, Smigocki, Ann, Vinterhalter, Dragan, "Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production" in Plant Cell Tissue and Organ Culture, 121, no. 3 (2015):667-679,
https://doi.org/10.1007/s11240-015-0737-z . .

TY  - GEN
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
AU  - Miljuš-Đukić, Jovanka D.
AU  - Jovanović, Z.
AU  - Orbović, V.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1949
AB  - Competence for phototropic (PT) and gravitropic (GT) bending by potato
   plantlets grown in vitro manifests regular daily changes indicating
   possible involvement of circadian regulation. Unilateral stimulation
   with the blue light of plantlets at dawn resulted in moderate PT
   response regarding both attained curvature and long lag phase. The PT
   response was the strongest between 8:00 and 12:00 h. Throughout the
   afternoon and in the evening, bending rate and maximal PT curvature
   declined significantly until 23:00 h. The GT response was fastest and
   strongest for plantlets stimulated early in the morning and late in the
   evening. During the rest of the day, GT competence did not change much
   apart from a minimum at 15:00. In conditions comprising either prolonged
   day or prolonged night, plantlets appeared to maintain rhythmicity of
   competence for PT and GT at least in a short-term. Introduction of dark
   period prior to the tropic stimulation at 11:00 h when both PT and GT
   responses were strong resulted in the opposite effect: PT was depressed
   and GT was enhanced. There was a time threshold of 60 min for the
   duration of dark period so the plants can sense interruption in the
   daylight. Levels of relative expression of a PHOT2 gene indicate
   rhythmic daily changes. PHOT2 gene was present at high levels during
   morning hours and late in the evening. As the mid-day and the afternoon
   hours approached, PHOT2 expression decreased and reached daily minimum
   at 17:00 h. We believe that our data offer strong support for the
   conclusion that there is an involvement of circadian rhythms in control
   of both PT and GT.
T2  - Journal of Plant Growth Regulation
T1  - Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014)
IS  - 2
VL  - 34
DO  - 10.1007/s00344-015-9507-8
SP  - 440
EP  - 450
ER  - 

@misc{
author = "Vinterhalter, Dragan and Vinterhalter, Branka and Miljuš-Đukić, Jovanka D. and Jovanović, Z. and Orbović, V.",
year = "2015",
abstract = "Competence for phototropic (PT) and gravitropic (GT) bending by potato
   plantlets grown in vitro manifests regular daily changes indicating
   possible involvement of circadian regulation. Unilateral stimulation
   with the blue light of plantlets at dawn resulted in moderate PT
   response regarding both attained curvature and long lag phase. The PT
   response was the strongest between 8:00 and 12:00 h. Throughout the
   afternoon and in the evening, bending rate and maximal PT curvature
   declined significantly until 23:00 h. The GT response was fastest and
   strongest for plantlets stimulated early in the morning and late in the
   evening. During the rest of the day, GT competence did not change much
   apart from a minimum at 15:00. In conditions comprising either prolonged
   day or prolonged night, plantlets appeared to maintain rhythmicity of
   competence for PT and GT at least in a short-term. Introduction of dark
   period prior to the tropic stimulation at 11:00 h when both PT and GT
   responses were strong resulted in the opposite effect: PT was depressed
   and GT was enhanced. There was a time threshold of 60 min for the
   duration of dark period so the plants can sense interruption in the
   daylight. Levels of relative expression of a PHOT2 gene indicate
   rhythmic daily changes. PHOT2 gene was present at high levels during
   morning hours and late in the evening. As the mid-day and the afternoon
   hours approached, PHOT2 expression decreased and reached daily minimum
   at 17:00 h. We believe that our data offer strong support for the
   conclusion that there is an involvement of circadian rhythms in control
   of both PT and GT.",
journal = "Journal of Plant Growth Regulation",
title = "Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014)",
number = "2",
volume = "34",
doi = "10.1007/s00344-015-9507-8",
pages = "440-450"
}

Vinterhalter, D., Vinterhalter, B., Miljuš-Đukić, J. D., Jovanović, Z.,& Orbović, V.. (2015). Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014). in Journal of Plant Growth Regulation, 34(2), 440-450.
https://doi.org/10.1007/s00344-015-9507-8

Vinterhalter D, Vinterhalter B, Miljuš-Đukić JD, Jovanović Z, Orbović V. Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014). in Journal of Plant Growth Regulation. 2015;34(2):440-450.
doi:10.1007/s00344-015-9507-8 .

Vinterhalter, Dragan, Vinterhalter, Branka, Miljuš-Đukić, Jovanka D., Jovanović, Z., Orbović, V., "Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014)" in Journal of Plant Growth Regulation, 34, no. 2 (2015):440-450,
https://doi.org/10.1007/s00344-015-9507-8 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Raspor, Martin
AU  - Savic, Jelena
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
AU  - Travnickova, Alena
AU  - Dobrev, Petre I.
AU  - Bohanec, Borut
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1956
AB  - Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
   (VP) and Vienna White (VW) were tested for their ability of de novo
   organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
   seedlings were cultivated on Murashige and Skoog (MS) media supplemented
   with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
   or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
   shoot regeneration from hypocotyl explants and intact seedlings, with
   seedlings being most successful for regeneration efficiency and
   viability of regenerated shoots in both cultivars. The highest frequency
   of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
   for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
   Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
   contents in both hypocotyl explants and seedlings with regenerated
   shoots (HRSs and SRSs) suggested that the observed differences in
   organogenic response between these two types of explants were related to
   their cytokinin and IAA contents. HRSs generally exhibited elevated
   amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
   cytokinins ratio. Shoots regenerated from seedlings were further
   successfully multiplicated on a medium supplemented with BA (0.5 mg
   L-1). The rooting potential of multiplicated shoots was tested on media
   supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
   higher concentration of IBA leading to more efficient rooting. Rooted
   plantlets were successfully planted into soil and flow cytometric
   analysis did not reveal ploidy variations, indicating that the described
   protocol is fast and efficient for kohlrabi regeneration.
T2  - Plant Cell Tissue and Organ Culture
T1  - In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins
IS  - 3
VL  - 121
DO  - 10.1007/s11240-015-0742-2
SP  - 741
EP  - 760
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Vaclav and Raspor, Martin and Savic, Jelena and Cingel, Aleksandar and Vinterhalter, Branka and Vinterhalter, Dragan and Travnickova, Alena and Dobrev, Petre I. and Bohanec, Borut and Ninković, Slavica",
year = "2015",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
   (VP) and Vienna White (VW) were tested for their ability of de novo
   organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
   seedlings were cultivated on Murashige and Skoog (MS) media supplemented
   with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
   or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
   shoot regeneration from hypocotyl explants and intact seedlings, with
   seedlings being most successful for regeneration efficiency and
   viability of regenerated shoots in both cultivars. The highest frequency
   of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
   for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
   Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
   contents in both hypocotyl explants and seedlings with regenerated
   shoots (HRSs and SRSs) suggested that the observed differences in
   organogenic response between these two types of explants were related to
   their cytokinin and IAA contents. HRSs generally exhibited elevated
   amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
   cytokinins ratio. Shoots regenerated from seedlings were further
   successfully multiplicated on a medium supplemented with BA (0.5 mg
   L-1). The rooting potential of multiplicated shoots was tested on media
   supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
   higher concentration of IBA leading to more efficient rooting. Rooted
   plantlets were successfully planted into soil and flow cytometric
   analysis did not reveal ploidy variations, indicating that the described
   protocol is fast and efficient for kohlrabi regeneration.",
journal = "Plant Cell Tissue and Organ Culture",
title = "In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins",
number = "3",
volume = "121",
doi = "10.1007/s11240-015-0742-2",
pages = "741-760"
}

Ćosić, T., Motyka, V., Raspor, M., Savic, J., Cingel, A., Vinterhalter, B., Vinterhalter, D., Travnickova, A., Dobrev, P. I., Bohanec, B.,& Ninković, S.. (2015). In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins. in Plant Cell Tissue and Organ Culture, 121(3), 741-760.
https://doi.org/10.1007/s11240-015-0742-2

Ćosić T, Motyka V, Raspor M, Savic J, Cingel A, Vinterhalter B, Vinterhalter D, Travnickova A, Dobrev PI, Bohanec B, Ninković S. In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins. in Plant Cell Tissue and Organ Culture. 2015;121(3):741-760.
doi:10.1007/s11240-015-0742-2 .

Ćosić, Tatjana, Motyka, Vaclav, Raspor, Martin, Savic, Jelena, Cingel, Aleksandar, Vinterhalter, Branka, Vinterhalter, Dragan, Travnickova, Alena, Dobrev, Petre I., Bohanec, Borut, Ninković, Slavica, "In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins" in Plant Cell Tissue and Organ Culture, 121, no. 3 (2015):741-760,
https://doi.org/10.1007/s11240-015-0742-2 . .

TY  - JOUR
AU  - Cingel, Aleksandar
AU  - Savic, Jelena
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
AU  - Kostic, Miroslav
AU  - Šešlija Jovanović, Darka
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1923
AB  - Plant proteinase inhibitors (PIs) are attractive tools for crop
   improvement and their heterologous expression can enhance insect
   resistance in transgenic plants. PI oryzacystatin II (OCII), isolated
   from rice, showed potential in controlling pests that utilize cysteine
   proteinases for protein digestion. To evaluate the applicability of the
   OCII gene in enhancing plant defence, OCII-transformed potatoes were
   bioassayed for resistance to Colorado potato beetle (Leptinotarsa
   decemlineata Say). Feeding on transformed leaves of potato cultivars
   Desiree and Jelica significantly affected larval growth and development,
   but did not change mortality rates. During the L2 and L3 developmental
   stages larvae consumed the OCII-transformed foliage faster as compared
   to the nontransformed control. Also these larvae reached the prepupal
   stage (end of L4 stage) 2 days earlier than those fed on control leaves.
   However, the total amounts of consumed OCII-transformed leaves were up
   to 23 \% lower than of control, and the maximal weights of prepupal
   larvae were reduced by up to 18 \% as compared to larvae fed on
   nontransformed leaves. The reduction in insect fitness reported in this
   study in combination with other control measures, could lead to improved
   CPB resistance management in potato.
T2  - Transgenic Research
T1  - Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor
IS  - 4
VL  - 24
DO  - 10.1007/s11248-015-9873-9
SP  - 729
EP  - 740
ER  - 

@article{
author = "Cingel, Aleksandar and Savic, Jelena and Vinterhalter, Branka and Vinterhalter, Dragan and Kostic, Miroslav and Šešlija Jovanović, Darka and Smigocki, Ann and Ninković, Slavica",
year = "2015",
abstract = "Plant proteinase inhibitors (PIs) are attractive tools for crop
   improvement and their heterologous expression can enhance insect
   resistance in transgenic plants. PI oryzacystatin II (OCII), isolated
   from rice, showed potential in controlling pests that utilize cysteine
   proteinases for protein digestion. To evaluate the applicability of the
   OCII gene in enhancing plant defence, OCII-transformed potatoes were
   bioassayed for resistance to Colorado potato beetle (Leptinotarsa
   decemlineata Say). Feeding on transformed leaves of potato cultivars
   Desiree and Jelica significantly affected larval growth and development,
   but did not change mortality rates. During the L2 and L3 developmental
   stages larvae consumed the OCII-transformed foliage faster as compared
   to the nontransformed control. Also these larvae reached the prepupal
   stage (end of L4 stage) 2 days earlier than those fed on control leaves.
   However, the total amounts of consumed OCII-transformed leaves were up
   to 23 \% lower than of control, and the maximal weights of prepupal
   larvae were reduced by up to 18 \% as compared to larvae fed on
   nontransformed leaves. The reduction in insect fitness reported in this
   study in combination with other control measures, could lead to improved
   CPB resistance management in potato.",
journal = "Transgenic Research",
title = "Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor",
number = "4",
volume = "24",
doi = "10.1007/s11248-015-9873-9",
pages = "729-740"
}

Cingel, A., Savic, J., Vinterhalter, B., Vinterhalter, D., Kostic, M., Šešlija Jovanović, D., Smigocki, A.,& Ninković, S.. (2015). Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor. in Transgenic Research, 24(4), 729-740.
https://doi.org/10.1007/s11248-015-9873-9

Cingel A, Savic J, Vinterhalter B, Vinterhalter D, Kostic M, Šešlija Jovanović D, Smigocki A, Ninković S. Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor. in Transgenic Research. 2015;24(4):729-740.
doi:10.1007/s11248-015-9873-9 .

Cingel, Aleksandar, Savic, Jelena, Vinterhalter, Branka, Vinterhalter, Dragan, Kostic, Miroslav, Šešlija Jovanović, Darka, Smigocki, Ann, Ninković, Slavica, "Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor" in Transgenic Research, 24, no. 4 (2015):729-740,
https://doi.org/10.1007/s11248-015-9873-9 . .

TY  - JOUR
AU  - Momčilović, Ivana
AU  - Pantelic, Danijel
AU  - Hfidan, Masoud
AU  - Savic, Jelena
AU  - Vinterhalter, Dragan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2180
AB  - Superoxide dismutase (SOD) in-gel activity assay with selective
   inhibitors (KCN and H2O2) is one of the most commonly used methods for
   identification of SOD isoform types, i.e., FeSOD, MnSOD or Cu/ZnSOD, and
   evaluation of oxidative stress response in plants. However, there are
   potential pitfalls that surround this assay, such as problem to detect
   isoforms with low activity, comigration of SOD isoforms or application
   of inappropriate inhibitor concentration. We propose an improved method
   based on the combination of in-gel analysis of SOD activity and
   native-PAGE immunoblotting for identification of isoforms and
   determination of SOD isoenzyme activity pattern in potato. Depending on
   cultivar and growing conditions, one MnSOD, 3 FeSOD and 5-6 Cu/ZnSOD
   isoforms were identified in potato leaves. The most important
   qualitative difference between ex vitro- and in vitro-grown plants was
   the presence of additional FeSOD and Cu/ZnSOD isoforms in plantlets
   grown in vitro. Compared with results of in-gel activity assay with
   selective inhibitors, new method allowed accurate identification of
   comigrating FeSOD and Cu/ZnSOD isoforms and two protein bands of
   ambiguous identities. Potato SODs were also characterized by SDS-PAGE
   immunoblotting and single MnSOD (23.6 kDa), three Cu/ZnSOD polypeptides
   (17.9, 17 and 16.3 kDa) and single FeSOD (25.1 kDa) polypeptide were
   detected in leaves of four examined cultivars. The difference in the
   number of FeSOD and Cu/ZnSOD isoforms/polypeptides between native-PAGE
   and SDS-PAGE immunoblots suggests that SOD proteins may have undergone
   post-translational modifications affecting protein mobility or existence
   of isoforms that differ from each other in total protein charge, but not
   in molecular weight.
T2  - Acta Physiologiae Plantarum
T1  - Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.
IS  - 8
VL  - 36
DO  - 10.1007/s11738-014-1583-z
SP  - 2059
EP  - 2066
ER  - 

@article{
author = "Momčilović, Ivana and Pantelic, Danijel and Hfidan, Masoud and Savic, Jelena and Vinterhalter, Dragan",
year = "2014",
abstract = "Superoxide dismutase (SOD) in-gel activity assay with selective
   inhibitors (KCN and H2O2) is one of the most commonly used methods for
   identification of SOD isoform types, i.e., FeSOD, MnSOD or Cu/ZnSOD, and
   evaluation of oxidative stress response in plants. However, there are
   potential pitfalls that surround this assay, such as problem to detect
   isoforms with low activity, comigration of SOD isoforms or application
   of inappropriate inhibitor concentration. We propose an improved method
   based on the combination of in-gel analysis of SOD activity and
   native-PAGE immunoblotting for identification of isoforms and
   determination of SOD isoenzyme activity pattern in potato. Depending on
   cultivar and growing conditions, one MnSOD, 3 FeSOD and 5-6 Cu/ZnSOD
   isoforms were identified in potato leaves. The most important
   qualitative difference between ex vitro- and in vitro-grown plants was
   the presence of additional FeSOD and Cu/ZnSOD isoforms in plantlets
   grown in vitro. Compared with results of in-gel activity assay with
   selective inhibitors, new method allowed accurate identification of
   comigrating FeSOD and Cu/ZnSOD isoforms and two protein bands of
   ambiguous identities. Potato SODs were also characterized by SDS-PAGE
   immunoblotting and single MnSOD (23.6 kDa), three Cu/ZnSOD polypeptides
   (17.9, 17 and 16.3 kDa) and single FeSOD (25.1 kDa) polypeptide were
   detected in leaves of four examined cultivars. The difference in the
   number of FeSOD and Cu/ZnSOD isoforms/polypeptides between native-PAGE
   and SDS-PAGE immunoblots suggests that SOD proteins may have undergone
   post-translational modifications affecting protein mobility or existence
   of isoforms that differ from each other in total protein charge, but not
   in molecular weight.",
journal = "Acta Physiologiae Plantarum",
title = "Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.",
number = "8",
volume = "36",
doi = "10.1007/s11738-014-1583-z",
pages = "2059-2066"
}

Momčilović, I., Pantelic, D., Hfidan, M., Savic, J.,& Vinterhalter, D.. (2014). Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.. in Acta Physiologiae Plantarum, 36(8), 2059-2066.
https://doi.org/10.1007/s11738-014-1583-z

Momčilović I, Pantelic D, Hfidan M, Savic J, Vinterhalter D. Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.. in Acta Physiologiae Plantarum. 2014;36(8):2059-2066.
doi:10.1007/s11738-014-1583-z .

Momčilović, Ivana, Pantelic, Danijel, Hfidan, Masoud, Savic, Jelena, Vinterhalter, Dragan, "Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L." in Acta Physiologiae Plantarum, 36, no. 8 (2014):2059-2066,
https://doi.org/10.1007/s11738-014-1583-z . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Janković, Teodora
AU  - Miljuš-Đukić, Jovanka
AU  - Vinterhalter, Dragan
PY  - 2011
UR  - https://bp.ueb.cas.cz/pdfs/bpl/2011/03/02.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6167
AB  - Differences among three clones of Gentiana punctata L. hairy root shoot regenerants were investigated in relation to their growth patterns, production of secondary metabolites and 2D protein profiles. Prominent differences in growth parameters were stable thus qualifying regenerant clones as true somaclones. Marked differences in protein spots were registered among the regenerant clones but not in comparison with the non-transformed control. Southern blot hybridization of regenerants showed the absence of rolA, B and C genes, initially present in the main hairy root lines. Orf13 and rolD were present and orf8 was missing in all three regenerant clones whereas orf3 was missing only in clone 2. Although lacking the three major rol genes, plants of regenerant clones retained characteristics of the hairy root phenotype.
PB  - Dordrecht: Springer-Verlag
T2  - Biologia Plantarum
T1  - Variability in shoot cultures of hairy root regenerated Gentiana punctata L.
IS  - 3
VL  - 55
DO  - 10.1007/s10535-011-0105-8
SP  - 414
EP  - 422
ER  - 

@article{
author = "Vinterhalter, Branka and Zdravković-Korać, Snežana and Ninković, Slavica and Banjac, Nevena and Janković, Teodora and Miljuš-Đukić, Jovanka and Vinterhalter, Dragan",
year = "2011",
abstract = "Differences among three clones of Gentiana punctata L. hairy root shoot regenerants were investigated in relation to their growth patterns, production of secondary metabolites and 2D protein profiles. Prominent differences in growth parameters were stable thus qualifying regenerant clones as true somaclones. Marked differences in protein spots were registered among the regenerant clones but not in comparison with the non-transformed control. Southern blot hybridization of regenerants showed the absence of rolA, B and C genes, initially present in the main hairy root lines. Orf13 and rolD were present and orf8 was missing in all three regenerant clones whereas orf3 was missing only in clone 2. Although lacking the three major rol genes, plants of regenerant clones retained characteristics of the hairy root phenotype.",
publisher = "Dordrecht: Springer-Verlag",
journal = "Biologia Plantarum",
title = "Variability in shoot cultures of hairy root regenerated Gentiana punctata L.",
number = "3",
volume = "55",
doi = "10.1007/s10535-011-0105-8",
pages = "414-422"
}

Vinterhalter, B., Zdravković-Korać, S., Ninković, S., Banjac, N., Janković, T., Miljuš-Đukić, J.,& Vinterhalter, D.. (2011). Variability in shoot cultures of hairy root regenerated Gentiana punctata L.. in Biologia Plantarum
Dordrecht: Springer-Verlag., 55(3), 414-422.
https://doi.org/10.1007/s10535-011-0105-8

Vinterhalter B, Zdravković-Korać S, Ninković S, Banjac N, Janković T, Miljuš-Đukić J, Vinterhalter D. Variability in shoot cultures of hairy root regenerated Gentiana punctata L.. in Biologia Plantarum. 2011;55(3):414-422.
doi:10.1007/s10535-011-0105-8 .

Vinterhalter, Branka, Zdravković-Korać, Snežana, Ninković, Slavica, Banjac, Nevena, Janković, Teodora, Miljuš-Đukić, Jovanka, Vinterhalter, Dragan, "Variability in shoot cultures of hairy root regenerated Gentiana punctata L." in Biologia Plantarum, 55, no. 3 (2011):414-422,
https://doi.org/10.1007/s10535-011-0105-8 . .

TY  - JOUR
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
AU  - Miljuš-Đukić, Jovanka
AU  - Ninković, Slavica
PY  - 2009
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/363
AB  - The aim of this study was to find the most suitable protocol for the in vitro regeneration of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragačevka and cv. Jelica), as a prerequisite for further transformation studies. Three different protocols using leaf segments as starting explants were tested. The regeneration efficiency depending on a procedure and a genotype ranged from 41% to 89%, 46% to 92% and from 28% to 57% for Desiree, Dragačevka and Jelica, respectively. The two-step protocol included: (1) the induction of calli on the basal Murashige and Skoog (MS) medium with 0.2 mg l-1 NAA and 2.0 mg l-1 BAP and (2) the regeneration of shoots on the basal medium with 1.5% sucrose, 2.0 mg l-1 BAP and 5 mg l-1 GA3. It was determined that this protocol was the most efficient one for the plant regeneration. This protocol could be successfully applied in the transformation of Dragačevka and Jelica cultivars with Agrobacerium tumfaciens strains. .
AB  - U ovom radu su testirana tri protokola za regeneraciju in vitro domaćih sorti krompira dragačevke i jelice iz odsečaka listova. Efikasnost regeneracije je zavisila od genotipa i procedure i iznosila je 41-89% za sortu Desiree, 46-92% za sortu dragačevka i 28-57% za sortu jelica. Dvostepena procedura koja se sastojala iz (1) indukcije kalusa na osnovnoj Murashige and Skoog (MS) hranjivoj podlozi sa 0,2 mg/l NAA i 2,0 mg/l BAP i (2) regeneracije izdanaka na MS podlozi sa 1,5% saharoze, 2,0 mg/l BAP i 5,0 mg/l GA3 se pokazala kao najefikasnija. Ovaj postupak se može uspešno primeniti u transformaciji domaćih kultivara pomoću sojeva Agrobacterium tumfaciens. .
T2  - Journal of Scientific Agricultural Research
T1  - Efikasna regeneracija domaćih sorti krompira (Solanum tuberosum L.- Dragačevka i Jelica) u kulturi in vitro
T1  - Efficient in vitro regeneration of domestic potato (Solanum tuberosum L.) cultivars Dragačevka and Jelica
IS  - 2
VL  - 70
SP  - 5
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_363
ER  - 

@article{
author = "Cingel, Aleksandar and Vinterhalter, Branka and Vinterhalter, Dragan and Miljuš-Đukić, Jovanka and Ninković, Slavica",
year = "2009, 2009",
abstract = "The aim of this study was to find the most suitable protocol for the in vitro regeneration of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragačevka and cv. Jelica), as a prerequisite for further transformation studies. Three different protocols using leaf segments as starting explants were tested. The regeneration efficiency depending on a procedure and a genotype ranged from 41% to 89%, 46% to 92% and from 28% to 57% for Desiree, Dragačevka and Jelica, respectively. The two-step protocol included: (1) the induction of calli on the basal Murashige and Skoog (MS) medium with 0.2 mg l-1 NAA and 2.0 mg l-1 BAP and (2) the regeneration of shoots on the basal medium with 1.5% sucrose, 2.0 mg l-1 BAP and 5 mg l-1 GA3. It was determined that this protocol was the most efficient one for the plant regeneration. This protocol could be successfully applied in the transformation of Dragačevka and Jelica cultivars with Agrobacerium tumfaciens strains. ., U ovom radu su testirana tri protokola za regeneraciju in vitro domaćih sorti krompira dragačevke i jelice iz odsečaka listova. Efikasnost regeneracije je zavisila od genotipa i procedure i iznosila je 41-89% za sortu Desiree, 46-92% za sortu dragačevka i 28-57% za sortu jelica. Dvostepena procedura koja se sastojala iz (1) indukcije kalusa na osnovnoj Murashige and Skoog (MS) hranjivoj podlozi sa 0,2 mg/l NAA i 2,0 mg/l BAP i (2) regeneracije izdanaka na MS podlozi sa 1,5% saharoze, 2,0 mg/l BAP i 5,0 mg/l GA3 se pokazala kao najefikasnija. Ovaj postupak se može uspešno primeniti u transformaciji domaćih kultivara pomoću sojeva Agrobacterium tumfaciens. .",
journal = "Journal of Scientific Agricultural Research",
title = "Efikasna regeneracija domaćih sorti krompira (Solanum tuberosum L.- Dragačevka i Jelica) u kulturi in vitro, Efficient in vitro regeneration of domestic potato (Solanum tuberosum L.) cultivars Dragačevka and Jelica",
number = "2",
volume = "70",
pages = "5-14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_363"
}

Cingel, A., Vinterhalter, B., Vinterhalter, D., Miljuš-Đukić, J.,& Ninković, S.. (2009). Efikasna regeneracija domaćih sorti krompira (Solanum tuberosum L.- Dragačevka i Jelica) u kulturi in vitro. in Journal of Scientific Agricultural Research, 70(2), 5-14.
https://hdl.handle.net/21.15107/rcub_ibiss_363

Cingel A, Vinterhalter B, Vinterhalter D, Miljuš-Đukić J, Ninković S. Efikasna regeneracija domaćih sorti krompira (Solanum tuberosum L.- Dragačevka i Jelica) u kulturi in vitro. in Journal of Scientific Agricultural Research. 2009;70(2):5-14.
https://hdl.handle.net/21.15107/rcub_ibiss_363 .

Cingel, Aleksandar, Vinterhalter, Branka, Vinterhalter, Dragan, Miljuš-Đukić, Jovanka, Ninković, Slavica, "Efikasna regeneracija domaćih sorti krompira (Solanum tuberosum L.- Dragačevka i Jelica) u kulturi in vitro" in Journal of Scientific Agricultural Research, 70, no. 2 (2009):5-14,
https://hdl.handle.net/21.15107/rcub_ibiss_363 .