TY  - JOUR
AU  - Pajić, Tanja
AU  - Stevanović, Katarina
AU  - Todorović, Nataša
AU  - Krmpot, Aleksandar J
AU  - Živić, Miroslav
AU  - Savić-Šević, Svetlana
AU  - Lević, Steva M
AU  - Stanić, Marina
AU  - Pantelić, Dejan
AU  - Jelenković, Brana
AU  - Rabasović, Mihailo D
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6666
AB  - Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens. However, their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell wall. Here, we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi, which enabled patch-clamp measurements on protoplasts released from hyphae. A reproducible and highly precise (diffraction-limited, submicron resolution) method for obtaining viable released protoplasts was developed. Protoplast release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the hyphae. The plasma membrane of the obtained protoplasts formed tight and high-resistance (GΩ) contacts with the recording pipette. The entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 hour. Compared to previous studies using heterologously expressed channels, this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.
PB  - Springer Nature
T2  - Microsystems & Nanoengineering
T1  - In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi
VL  - 10
DO  - 10.1038/s41378-024-00664-x
SP  - 47
ER  - 

@article{
author = "Pajić, Tanja and Stevanović, Katarina and Todorović, Nataša and Krmpot, Aleksandar J and Živić, Miroslav and Savić-Šević, Svetlana and Lević, Steva M and Stanić, Marina and Pantelić, Dejan and Jelenković, Brana and Rabasović, Mihailo D",
year = "2024",
abstract = "Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens. However, their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell wall. Here, we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi, which enabled patch-clamp measurements on protoplasts released from hyphae. A reproducible and highly precise (diffraction-limited, submicron resolution) method for obtaining viable released protoplasts was developed. Protoplast release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the hyphae. The plasma membrane of the obtained protoplasts formed tight and high-resistance (GΩ) contacts with the recording pipette. The entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 hour. Compared to previous studies using heterologously expressed channels, this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.",
publisher = "Springer Nature",
journal = "Microsystems & Nanoengineering",
title = "In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi",
volume = "10",
doi = "10.1038/s41378-024-00664-x",
pages = "47"
}

Pajić, T., Stevanović, K., Todorović, N., Krmpot, A. J., Živić, M., Savić-Šević, S., Lević, S. M., Stanić, M., Pantelić, D., Jelenković, B.,& Rabasović, M. D.. (2024). In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi. in Microsystems & Nanoengineering
Springer Nature., 10, 47.
https://doi.org/10.1038/s41378-024-00664-x

Pajić T, Stevanović K, Todorović N, Krmpot AJ, Živić M, Savić-Šević S, Lević SM, Stanić M, Pantelić D, Jelenković B, Rabasović MD. In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi. in Microsystems & Nanoengineering. 2024;10:47.
doi:10.1038/s41378-024-00664-x .

Pajić, Tanja, Stevanović, Katarina, Todorović, Nataša, Krmpot, Aleksandar J, Živić, Miroslav, Savić-Šević, Svetlana, Lević, Steva M, Stanić, Marina, Pantelić, Dejan, Jelenković, Brana, Rabasović, Mihailo D, "In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi" in Microsystems & Nanoengineering, 10 (2024):47,
https://doi.org/10.1038/s41378-024-00664-x . .

TY  - JOUR
AU  - Jakovljević, Olga
AU  - Krizmanić, Jelena
AU  - Vasiljević, Božica
AU  - Popović, Slađana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6641
AB  - Some of the direct and indirect environmental impacts are related to the construction of fish farms (FFs) and small hydropower plants (SHPs) around the world. Diatom communities are one of the best bioindicators of various pressures like eutrophication, chemical and organic pollution. The main objective of this study was to examine the influence of SHP and FF on diatom community and water quality of the Prištavica River in Serbia. A total of 184 epilithic diatom taxa from 55 genera were identified. Sites after the FF and SHP are distinguished by the same dominant species in each season. Compared to the other sites, species diversity in summer was lower at site after SHP. In terms of spatial dynamics, four groups of taxa are distinguished. Some centric taxa were associated with sites downstream the FF and SHP. Altered physical and chemical parameters were documented at sites downstream of constructions. Water quality of the Prištavica River ranged from high to bad depending on diatom index, season, and sampling site. A greater difference in diatom index values was found at sites upstream and downstream of the FF, than at sites upstream and downstream of the SHP.
PB  - EDP Sciences
T2  - International Journal of Limnology
T1  - The response of diatom community to small hydropower plant and fish farm
VL  - 60
DO  - 10.1051/limn/2024004
SP  - 4
ER  - 

@article{
author = "Jakovljević, Olga and Krizmanić, Jelena and Vasiljević, Božica and Popović, Slađana",
year = "2024",
abstract = "Some of the direct and indirect environmental impacts are related to the construction of fish farms (FFs) and small hydropower plants (SHPs) around the world. Diatom communities are one of the best bioindicators of various pressures like eutrophication, chemical and organic pollution. The main objective of this study was to examine the influence of SHP and FF on diatom community and water quality of the Prištavica River in Serbia. A total of 184 epilithic diatom taxa from 55 genera were identified. Sites after the FF and SHP are distinguished by the same dominant species in each season. Compared to the other sites, species diversity in summer was lower at site after SHP. In terms of spatial dynamics, four groups of taxa are distinguished. Some centric taxa were associated with sites downstream the FF and SHP. Altered physical and chemical parameters were documented at sites downstream of constructions. Water quality of the Prištavica River ranged from high to bad depending on diatom index, season, and sampling site. A greater difference in diatom index values was found at sites upstream and downstream of the FF, than at sites upstream and downstream of the SHP.",
publisher = "EDP Sciences",
journal = "International Journal of Limnology",
title = "The response of diatom community to small hydropower plant and fish farm",
volume = "60",
doi = "10.1051/limn/2024004",
pages = "4"
}

Jakovljević, O., Krizmanić, J., Vasiljević, B.,& Popović, S.. (2024). The response of diatom community to small hydropower plant and fish farm. in International Journal of Limnology
EDP Sciences., 60, 4.
https://doi.org/10.1051/limn/2024004

Jakovljević O, Krizmanić J, Vasiljević B, Popović S. The response of diatom community to small hydropower plant and fish farm. in International Journal of Limnology. 2024;60:4.
doi:10.1051/limn/2024004 .

Jakovljević, Olga, Krizmanić, Jelena, Vasiljević, Božica, Popović, Slađana, "The response of diatom community to small hydropower plant and fish farm" in International Journal of Limnology, 60 (2024):4,
https://doi.org/10.1051/limn/2024004 . .

TY  - JOUR
AU  - Roljić, Rajko L.
AU  - Nikolić, Vera P.
AU  - Đikanović, Vesna
AU  - Zorić, Katarina
AU  - Urošević, Aleksandar
AU  - Marković, Vanja M.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6537
AB  - Abstract: Twenty-three morphometric characteristics of spiny-cheek crayfish Faxonus limosus from Danube, Serbia, were analyzed to describe the general body shape. Forty-eight specimens were caught in May 2022 and January 2023 and measured for morphometric variation. Also, the values of two crayfish condition indices, Fulton’s condition factor (FCF) and crayfish constant (CC), were determined. Most of the analyzed characteristics were moderately variable (CV 10-30%). Principal component analysis (PCA) was applied to the morphological measurements. All the variables make similar contributions to the variance of the first principal component. The highest contributions are from variables CEF and ARL.ARW, CPH, ABH, CPW, and ROW significantly contributed to the second principal component variance. The population of spiny-cheek crayfish at the investigated site has a relatively stable age structure.
PB  - Belgrade: Serbian Biological Socitey
T2  - Archives of Biological Sciences
T1  - Morphometric characteristics of spiny-cheek crayfish Faxonius limosus (Rafinesque, 1817) from the Danube River on the territory of Serbia
DO  - 10.2298/ABS231212005R
ER  - 

@article{
author = "Roljić, Rajko L. and Nikolić, Vera P. and Đikanović, Vesna and Zorić, Katarina and Urošević, Aleksandar and Marković, Vanja M.",
year = "2024",
abstract = "Abstract: Twenty-three morphometric characteristics of spiny-cheek crayfish Faxonus limosus from Danube, Serbia, were analyzed to describe the general body shape. Forty-eight specimens were caught in May 2022 and January 2023 and measured for morphometric variation. Also, the values of two crayfish condition indices, Fulton’s condition factor (FCF) and crayfish constant (CC), were determined. Most of the analyzed characteristics were moderately variable (CV 10-30%). Principal component analysis (PCA) was applied to the morphological measurements. All the variables make similar contributions to the variance of the first principal component. The highest contributions are from variables CEF and ARL.ARW, CPH, ABH, CPW, and ROW significantly contributed to the second principal component variance. The population of spiny-cheek crayfish at the investigated site has a relatively stable age structure.",
publisher = "Belgrade: Serbian Biological Socitey",
journal = "Archives of Biological Sciences",
title = "Morphometric characteristics of spiny-cheek crayfish Faxonius limosus (Rafinesque, 1817) from the Danube River on the territory of Serbia",
doi = "10.2298/ABS231212005R"
}

Roljić, R. L., Nikolić, V. P., Đikanović, V., Zorić, K., Urošević, A.,& Marković, V. M.. (2024). Morphometric characteristics of spiny-cheek crayfish Faxonius limosus (Rafinesque, 1817) from the Danube River on the territory of Serbia. in Archives of Biological Sciences
Belgrade: Serbian Biological Socitey..
https://doi.org/10.2298/ABS231212005R

Roljić RL, Nikolić VP, Đikanović V, Zorić K, Urošević A, Marković VM. Morphometric characteristics of spiny-cheek crayfish Faxonius limosus (Rafinesque, 1817) from the Danube River on the territory of Serbia. in Archives of Biological Sciences. 2024;.
doi:10.2298/ABS231212005R .

Roljić, Rajko L., Nikolić, Vera P., Đikanović, Vesna, Zorić, Katarina, Urošević, Aleksandar, Marković, Vanja M., "Morphometric characteristics of spiny-cheek crayfish Faxonius limosus (Rafinesque, 1817) from the Danube River on the territory of Serbia" in Archives of Biological Sciences (2024),
https://doi.org/10.2298/ABS231212005R . .

TY  - JOUR
AU  - Zakić, Tamara
AU  - Kalezić, Anđelika
AU  - Drvendžija, Zorka
AU  - Udicki, Mirjana
AU  - Ivković Kapicl, Tatjana
AU  - Srdić Galić, Biljana
AU  - Korać, Aleksandra
AU  - Janković, Aleksandra
AU  - Korać, Bato
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6528
AB  - The close cooperation between breast cancer and cancer-associated adipose tissue (CAAT) shapes the malignant phenotype, but the role of mitochondrial metabolic reprogramming and obesity in breast cancer remains undecided, especially in premenopausal women. Here, we examined mitochondrial metabolic dynamics in paired biopsies of malignant versus benign breast tumor tissue and CAAT in normal-weight and overweight/obese premenopausal women. Lower protein level of pyruvate dehydrogenase and citrate synthase in malignant tumor tissue indicated decreased carbon flux from glucose into the Krebs cycle, whereas the trend was just the opposite in malignant CAAT. Simultaneously, stimulated lipolysis in CAAT of obese women was followed by upregulated β-oxidation, as well as fatty acid synthesis enzymes in both tumor tissue and CAAT of women with malignant tumors, corroborating their physical association. Further, protein level of electron transport chain complexes was generally increased in tumor tissue and CAAT from women with malignant tumors, respective to obesity. Preserved mitochondrial structure in malignant tumor tissue was also observed. However, mitochondrial DNA copy number and protein levels of PGC-1α were dependent on both malignancy and obesity in tumor tissue and CAAT. In conclusion, metabolic cooperation between breast cancer and CAAT in premenopausal women involves obesity-related, synchronized changes in mitochondrial metabolism.
PB  - Basel: MDPI
T2  - Cells
T1  - Breast Cancer: Mitochondria-Centered Metabolic Alterations in Tumor and Associated Adipose Tissue
IS  - 2
VL  - 13
DO  - 10.3390/cells13020155
SP  - 155
ER  - 

@article{
author = "Zakić, Tamara and Kalezić, Anđelika and Drvendžija, Zorka and Udicki, Mirjana and Ivković Kapicl, Tatjana and Srdić Galić, Biljana and Korać, Aleksandra and Janković, Aleksandra and Korać, Bato",
year = "2024",
abstract = "The close cooperation between breast cancer and cancer-associated adipose tissue (CAAT) shapes the malignant phenotype, but the role of mitochondrial metabolic reprogramming and obesity in breast cancer remains undecided, especially in premenopausal women. Here, we examined mitochondrial metabolic dynamics in paired biopsies of malignant versus benign breast tumor tissue and CAAT in normal-weight and overweight/obese premenopausal women. Lower protein level of pyruvate dehydrogenase and citrate synthase in malignant tumor tissue indicated decreased carbon flux from glucose into the Krebs cycle, whereas the trend was just the opposite in malignant CAAT. Simultaneously, stimulated lipolysis in CAAT of obese women was followed by upregulated β-oxidation, as well as fatty acid synthesis enzymes in both tumor tissue and CAAT of women with malignant tumors, corroborating their physical association. Further, protein level of electron transport chain complexes was generally increased in tumor tissue and CAAT from women with malignant tumors, respective to obesity. Preserved mitochondrial structure in malignant tumor tissue was also observed. However, mitochondrial DNA copy number and protein levels of PGC-1α were dependent on both malignancy and obesity in tumor tissue and CAAT. In conclusion, metabolic cooperation between breast cancer and CAAT in premenopausal women involves obesity-related, synchronized changes in mitochondrial metabolism.",
publisher = "Basel: MDPI",
journal = "Cells",
title = "Breast Cancer: Mitochondria-Centered Metabolic Alterations in Tumor and Associated Adipose Tissue",
number = "2",
volume = "13",
doi = "10.3390/cells13020155",
pages = "155"
}

Zakić, T., Kalezić, A., Drvendžija, Z., Udicki, M., Ivković Kapicl, T., Srdić Galić, B., Korać, A., Janković, A.,& Korać, B.. (2024). Breast Cancer: Mitochondria-Centered Metabolic Alterations in Tumor and Associated Adipose Tissue. in Cells
Basel: MDPI., 13(2), 155.
https://doi.org/10.3390/cells13020155

Zakić T, Kalezić A, Drvendžija Z, Udicki M, Ivković Kapicl T, Srdić Galić B, Korać A, Janković A, Korać B. Breast Cancer: Mitochondria-Centered Metabolic Alterations in Tumor and Associated Adipose Tissue. in Cells. 2024;13(2):155.
doi:10.3390/cells13020155 .

Zakić, Tamara, Kalezić, Anđelika, Drvendžija, Zorka, Udicki, Mirjana, Ivković Kapicl, Tatjana, Srdić Galić, Biljana, Korać, Aleksandra, Janković, Aleksandra, Korać, Bato, "Breast Cancer: Mitochondria-Centered Metabolic Alterations in Tumor and Associated Adipose Tissue" in Cells, 13, no. 2 (2024):155,
https://doi.org/10.3390/cells13020155 . .

TY  - JOUR
AU  - Mavrikaki, Evangelia
AU  - Realdon, Giulia
AU  - Aivelo, Tuomas
AU  - Bajrami, Ani
AU  - Bakanay, Çiçek Dilek
AU  - Beniermann, Anna
AU  - Blagojević, Jelena
AU  - Butkeviciene, Egle
AU  - Cavadas, Bento
AU  - Cossu, Costantina
AU  - Cvetković, Dragana
AU  - Drobniak, Szymon M.
AU  - Durmuş, Zelal Özgür
AU  - Dvořáková, Radka Marta
AU  - Eens, Marcel
AU  - Eretq, Esra
AU  - Eroglu, Seckin
AU  - Gazda, Małgorzata Anna
AU  - Georgiou, Martha
AU  - Gostling, Neil J.
AU  - Gregorčič, Tanja
AU  - Janštová, Vanda
AU  - Jenkins, Tania
AU  - Kervinen, Anttoni
AU  - Korfiatis, Konstantinos
AU  - Kuschmierz, Paul
AU  - Lendvai, Ádám Z.
AU  - de Lima, Joelyn
AU  - Miri, Fundime
AU  - Nogueira, Teresa
AU  - Panayides, Andreas
AU  - Paolucci, Sylvia
AU  - Papadopoulou, Penelope
AU  - Pessoa, Patrícia
AU  - Pinxten, Rianne
AU  - Rios, Joana
AU  - Sousa, Bruno
AU  - Stasinakis, Panagiotis K.
AU  - Torkar, Gregor
AU  - Valackiene, Asta
AU  - Varga, Máté
AU  - Vázquez Ben, Lucía
AU  - Yarden, Anat
AU  - Sá-Pinto, Xana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6508
AB  - The contribution of school curricula to public understanding and acceptance of evolution is still mostly unknown, due to the scarcity of studies that compare the learning goals present in different curricula. To overcome this lack of data we analysed 19 school curricula (18 European and one from Israel) to study the
differences regarding the inclusion of learning goals targeting evolution understanding. We performed a quantitative content analysis using the Framework for the Assessment of school Curricula on the presence of Evolutionary concepts (FACE). For each country/region we analysed what this educational system considered the minimum evolution education a citizen should get. Our results reveal that: (i) the curricula include less than half of the learning goals considered important for scientific literacy in evolution; (ii) the most frequent learning goals address basic knowledge of evolution; (iii) learning goals related with the processes that drive evolution are often not included or rarely mentioned; (iv) evolution is most often not linked to its applications in everyday life. These results highlight the need to rethink evolution education across Europe.
PB  - Taylor & Francis Group
T2  - International Journal of Science Education
T1  - Evolution in European and Israeli school curricula – a comparative analysis
DO  - 10.1080/09500693.2023.2293090
ER  - 

@article{
author = "Mavrikaki, Evangelia and Realdon, Giulia and Aivelo, Tuomas and Bajrami, Ani and Bakanay, Çiçek Dilek and Beniermann, Anna and Blagojević, Jelena and Butkeviciene, Egle and Cavadas, Bento and Cossu, Costantina and Cvetković, Dragana and Drobniak, Szymon M. and Durmuş, Zelal Özgür and Dvořáková, Radka Marta and Eens, Marcel and Eretq, Esra and Eroglu, Seckin and Gazda, Małgorzata Anna and Georgiou, Martha and Gostling, Neil J. and Gregorčič, Tanja and Janštová, Vanda and Jenkins, Tania and Kervinen, Anttoni and Korfiatis, Konstantinos and Kuschmierz, Paul and Lendvai, Ádám Z. and de Lima, Joelyn and Miri, Fundime and Nogueira, Teresa and Panayides, Andreas and Paolucci, Sylvia and Papadopoulou, Penelope and Pessoa, Patrícia and Pinxten, Rianne and Rios, Joana and Sousa, Bruno and Stasinakis, Panagiotis K. and Torkar, Gregor and Valackiene, Asta and Varga, Máté and Vázquez Ben, Lucía and Yarden, Anat and Sá-Pinto, Xana",
year = "2024",
abstract = "The contribution of school curricula to public understanding and acceptance of evolution is still mostly unknown, due to the scarcity of studies that compare the learning goals present in different curricula. To overcome this lack of data we analysed 19 school curricula (18 European and one from Israel) to study the
differences regarding the inclusion of learning goals targeting evolution understanding. We performed a quantitative content analysis using the Framework for the Assessment of school Curricula on the presence of Evolutionary concepts (FACE). For each country/region we analysed what this educational system considered the minimum evolution education a citizen should get. Our results reveal that: (i) the curricula include less than half of the learning goals considered important for scientific literacy in evolution; (ii) the most frequent learning goals address basic knowledge of evolution; (iii) learning goals related with the processes that drive evolution are often not included or rarely mentioned; (iv) evolution is most often not linked to its applications in everyday life. These results highlight the need to rethink evolution education across Europe.",
publisher = "Taylor & Francis Group",
journal = "International Journal of Science Education",
title = "Evolution in European and Israeli school curricula – a comparative analysis",
doi = "10.1080/09500693.2023.2293090"
}

Mavrikaki, E., Realdon, G., Aivelo, T., Bajrami, A., Bakanay, Ç. D., Beniermann, A., Blagojević, J., Butkeviciene, E., Cavadas, B., Cossu, C., Cvetković, D., Drobniak, S. M., Durmuş, Z. Ö., Dvořáková, R. M., Eens, M., Eretq, E., Eroglu, S., Gazda, M. A., Georgiou, M., Gostling, N. J., Gregorčič, T., Janštová, V., Jenkins, T., Kervinen, A., Korfiatis, K., Kuschmierz, P., Lendvai, Á. Z., de Lima, J., Miri, F., Nogueira, T., Panayides, A., Paolucci, S., Papadopoulou, P., Pessoa, P., Pinxten, R., Rios, J., Sousa, B., Stasinakis, P. K., Torkar, G., Valackiene, A., Varga, M., Vázquez Ben, L., Yarden, A.,& Sá-Pinto, X.. (2024). Evolution in European and Israeli school curricula – a comparative analysis. in International Journal of Science Education
Taylor & Francis Group..
https://doi.org/10.1080/09500693.2023.2293090

Mavrikaki E, Realdon G, Aivelo T, Bajrami A, Bakanay ÇD, Beniermann A, Blagojević J, Butkeviciene E, Cavadas B, Cossu C, Cvetković D, Drobniak SM, Durmuş ZÖ, Dvořáková RM, Eens M, Eretq E, Eroglu S, Gazda MA, Georgiou M, Gostling NJ, Gregorčič T, Janštová V, Jenkins T, Kervinen A, Korfiatis K, Kuschmierz P, Lendvai ÁZ, de Lima J, Miri F, Nogueira T, Panayides A, Paolucci S, Papadopoulou P, Pessoa P, Pinxten R, Rios J, Sousa B, Stasinakis PK, Torkar G, Valackiene A, Varga M, Vázquez Ben L, Yarden A, Sá-Pinto X. Evolution in European and Israeli school curricula – a comparative analysis. in International Journal of Science Education. 2024;.
doi:10.1080/09500693.2023.2293090 .

Mavrikaki, Evangelia, Realdon, Giulia, Aivelo, Tuomas, Bajrami, Ani, Bakanay, Çiçek Dilek, Beniermann, Anna, Blagojević, Jelena, Butkeviciene, Egle, Cavadas, Bento, Cossu, Costantina, Cvetković, Dragana, Drobniak, Szymon M., Durmuş, Zelal Özgür, Dvořáková, Radka Marta, Eens, Marcel, Eretq, Esra, Eroglu, Seckin, Gazda, Małgorzata Anna, Georgiou, Martha, Gostling, Neil J., Gregorčič, Tanja, Janštová, Vanda, Jenkins, Tania, Kervinen, Anttoni, Korfiatis, Konstantinos, Kuschmierz, Paul, Lendvai, Ádám Z., de Lima, Joelyn, Miri, Fundime, Nogueira, Teresa, Panayides, Andreas, Paolucci, Sylvia, Papadopoulou, Penelope, Pessoa, Patrícia, Pinxten, Rianne, Rios, Joana, Sousa, Bruno, Stasinakis, Panagiotis K., Torkar, Gregor, Valackiene, Asta, Varga, Máté, Vázquez Ben, Lucía, Yarden, Anat, Sá-Pinto, Xana, "Evolution in European and Israeli school curricula – a comparative analysis" in International Journal of Science Education (2024),
https://doi.org/10.1080/09500693.2023.2293090 . .

TY  - DATA
AU  - Milanović, Slobodan
AU  - Milenković, Ivan
AU  - Lazarević, Jelica
AU  - Todosijević, Marina
AU  - Ljujić, Jovana
AU  - Mitić, Zorica
AU  - Nikolić, Biljana
AU  - Marin, Petar
AU  - Tešević, Vele
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6490
AB  - We provide one excel (with five sheets) and 5 csv files. The data in the excel file is the same as the one provided in the 5 csv files. Data file named 'raw data.xlsx' is consisted of 5 sheets containing raw data on all analyzed traits in the manuscript for the spongy moth and Phytophthora sp. The first sheet named 'RDC' contains data on relative deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The second sheet named 'ADC' contains data on absolute deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The third sheet named 'RCR' contains data on the relative consumption rate of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The forth sheet named ‘RGR’ contains data on relative growth rate of gypsy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The fifth sheet named ‘Ir’ contains data on the antimicrobial activity of three concentrations of Calocedrus decurrens and Cupressus arizonica essential oils against Phytophthora × cambivora, P. plurivora and P. quercina expressed as inhibition rate of mycelial growth.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6490
ER  - 

@misc{
author = "Milanović, Slobodan and Milenković, Ivan and Lazarević, Jelica and Todosijević, Marina and Ljujić, Jovana and Mitić, Zorica and Nikolić, Biljana and Marin, Petar and Tešević, Vele",
year = "2024",
abstract = "We provide one excel (with five sheets) and 5 csv files. The data in the excel file is the same as the one provided in the 5 csv files. Data file named 'raw data.xlsx' is consisted of 5 sheets containing raw data on all analyzed traits in the manuscript for the spongy moth and Phytophthora sp. The first sheet named 'RDC' contains data on relative deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The second sheet named 'ADC' contains data on absolute deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The third sheet named 'RCR' contains data on the relative consumption rate of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The forth sheet named ‘RGR’ contains data on relative growth rate of gypsy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The fifth sheet named ‘Ir’ contains data on the antimicrobial activity of three concentrations of Calocedrus decurrens and Cupressus arizonica essential oils against Phytophthora × cambivora, P. plurivora and P. quercina expressed as inhibition rate of mycelial growth.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6490"
}

Milanović, S., Milenković, I., Lazarević, J., Todosijević, M., Ljujić, J., Mitić, Z., Nikolić, B., Marin, P.,& Tešević, V.. (2024). Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6490

Milanović S, Milenković I, Lazarević J, Todosijević M, Ljujić J, Mitić Z, Nikolić B, Marin P, Tešević V. Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_6490 .

Milanović, Slobodan, Milenković, Ivan, Lazarević, Jelica, Todosijević, Marina, Ljujić, Jovana, Mitić, Zorica, Nikolić, Biljana, Marin, Petar, Tešević, Vele, "Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens" (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_6490 .

TY  - JOUR
AU  - Adžić Bukvić, Marija
AU  - Laketa, Danijela
AU  - Dragić, Milorad
AU  - Lavrnja, Irena
AU  - Nedeljković, Nadežda
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6368
AB  - Ecto-50-nucleotidase/CD73 (eN/CD73) is a membrane-bound enzyme involved in
extracellular production of adenosine and a cell adhesion molecule involved in cell–
cell interactions. In neuroinflammatory conditions such as experimental autoimmune
encephalomyelitis (EAE), reactive astrocytes occupying active demyelination areas
significantly upregulate eN/CD73 and express additional eN/CD73 variants. The present
study investigated whether the different eN/CD73 variants represent distinct
glycoforms and the functional consequences of their expression in neuroinflammatory
states. The study was performed in animals at different stages of EAE and in primary
astrocyte cultures treated with a range of inflammatory cytokines. Upregulation
at the mRNA, protein, and functional levels, as well as the appearance of multiple
eN/CD73 glycovariants were detected in the inflamed spinal cord tissue. At the peak
of the disease, eN/CD73 exhibited higher AMP turnover and lower enzymesubstrate
affinity than the control group, which was attributed to altered glycosylation
under neuroinflammatory conditions. A subsequent in vitro study showed that
primary astrocytes upregulated eN/CD73 and expressed the multiple glycovariants
upon stimulation with TNFα, IL-1β, IL-6, and ATP, with the effect occurring at least in
part via induction of JAK/STAT3 signaling. Experimental removal of glycan moieties
from membrane glycoproteins by PNGaseF decreased eN/CD73 activity but had no
effect on the enzyme's involvement in astrocyte migration. Our results suggest that
neuroinflammatory states are associated with the appearance of functionally distinct
eN/CD73 glycovariants, which may play a role in the development of the reactive
astrocyte phenotype.
PB  - Hoboken: Wiley
T2  - Glia
T1  - Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro
IS  - 1
VL  - 72
DO  - 10.1002/glia.24459
SP  - 19
EP  - 33
ER  - 

@article{
author = "Adžić Bukvić, Marija and Laketa, Danijela and Dragić, Milorad and Lavrnja, Irena and Nedeljković, Nadežda",
year = "2024",
abstract = "Ecto-50-nucleotidase/CD73 (eN/CD73) is a membrane-bound enzyme involved in
extracellular production of adenosine and a cell adhesion molecule involved in cell–
cell interactions. In neuroinflammatory conditions such as experimental autoimmune
encephalomyelitis (EAE), reactive astrocytes occupying active demyelination areas
significantly upregulate eN/CD73 and express additional eN/CD73 variants. The present
study investigated whether the different eN/CD73 variants represent distinct
glycoforms and the functional consequences of their expression in neuroinflammatory
states. The study was performed in animals at different stages of EAE and in primary
astrocyte cultures treated with a range of inflammatory cytokines. Upregulation
at the mRNA, protein, and functional levels, as well as the appearance of multiple
eN/CD73 glycovariants were detected in the inflamed spinal cord tissue. At the peak
of the disease, eN/CD73 exhibited higher AMP turnover and lower enzymesubstrate
affinity than the control group, which was attributed to altered glycosylation
under neuroinflammatory conditions. A subsequent in vitro study showed that
primary astrocytes upregulated eN/CD73 and expressed the multiple glycovariants
upon stimulation with TNFα, IL-1β, IL-6, and ATP, with the effect occurring at least in
part via induction of JAK/STAT3 signaling. Experimental removal of glycan moieties
from membrane glycoproteins by PNGaseF decreased eN/CD73 activity but had no
effect on the enzyme's involvement in astrocyte migration. Our results suggest that
neuroinflammatory states are associated with the appearance of functionally distinct
eN/CD73 glycovariants, which may play a role in the development of the reactive
astrocyte phenotype.",
publisher = "Hoboken: Wiley",
journal = "Glia",
title = "Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro",
number = "1",
volume = "72",
doi = "10.1002/glia.24459",
pages = "19-33"
}

Adžić Bukvić, M., Laketa, D., Dragić, M., Lavrnja, I.,& Nedeljković, N.. (2024). Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro. in Glia
Hoboken: Wiley., 72(1), 19-33.
https://doi.org/10.1002/glia.24459

Adžić Bukvić M, Laketa D, Dragić M, Lavrnja I, Nedeljković N. Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro. in Glia. 2024;72(1):19-33.
doi:10.1002/glia.24459 .

Adžić Bukvić, Marija, Laketa, Danijela, Dragić, Milorad, Lavrnja, Irena, Nedeljković, Nadežda, "Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro" in Glia, 72, no. 1 (2024):19-33,
https://doi.org/10.1002/glia.24459 . .

TY  - JOUR
AU  - Kasalović, Marijana P.
AU  - Jelača, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Lađarević, Jelena
AU  - Radovanović, Lidija
AU  - Božić, Bojan
AU  - Mijatović, Sanja
AU  - Pantelić, Nebojša Đ.
AU  - Kaluđerović, Goran N.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6268
AB  - Three new diphenyltin(IV) complexes, bis(3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoato)diphenyltin(IV) (1), bis(2-(4-methyl-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (2), and bis(2-(4-hydroxy-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (3), were synthesized and characterized by elemental microanalysis, FT-IR spectroscopy, and multinuclear (1H, 13C and 119Sn) NMR spectroscopy. Crystal structure of ligand precursor, 3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoic acid (HL1), has been determined by X-ray diffraction studies. Asymmetric bidentate coordination of the carboxylato ligands and skew trapezoidal structures are assumed for the synthesized complexes. In vitro anticancer activity of the synthesized diphenyltin(IV) complexes was evaluated against three human: MCF-7 (breast adenocarcinoma), A375 (melanoma), HCT116 (colorectal carcinoma), and three mouse tumor cell lines: 4 T1 (breast carcinoma), B16 (melanoma), CT26 (colon carcinoma) using MTT and CV assays. The IC50 values fall in the range from 0.1 to 3.7 μM. Flow cytometric analysis and fluorescent microscopy suggest that complex 1 induces caspase-dependent apoptosis followed with strong blockade of cell division in HCT116 cells. Since complex 1 showed ROS/RNS scavenging potential mentioned cytotoxicity was not connected with oxidative stress.
PB  - Elsevier
T2  - Journal of Inorganic Biochemistry
T1  - Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies
VL  - 250
DO  - 10.1016/j.jinorgbio.2023.112399
SP  - 112399
ER  - 

@article{
author = "Kasalović, Marijana P. and Jelača, Sanja and Maksimović-Ivanić, Danijela and Lađarević, Jelena and Radovanović, Lidija and Božić, Bojan and Mijatović, Sanja and Pantelić, Nebojša Đ. and Kaluđerović, Goran N.",
year = "2024",
abstract = "Three new diphenyltin(IV) complexes, bis(3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoato)diphenyltin(IV) (1), bis(2-(4-methyl-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (2), and bis(2-(4-hydroxy-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (3), were synthesized and characterized by elemental microanalysis, FT-IR spectroscopy, and multinuclear (1H, 13C and 119Sn) NMR spectroscopy. Crystal structure of ligand precursor, 3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoic acid (HL1), has been determined by X-ray diffraction studies. Asymmetric bidentate coordination of the carboxylato ligands and skew trapezoidal structures are assumed for the synthesized complexes. In vitro anticancer activity of the synthesized diphenyltin(IV) complexes was evaluated against three human: MCF-7 (breast adenocarcinoma), A375 (melanoma), HCT116 (colorectal carcinoma), and three mouse tumor cell lines: 4 T1 (breast carcinoma), B16 (melanoma), CT26 (colon carcinoma) using MTT and CV assays. The IC50 values fall in the range from 0.1 to 3.7 μM. Flow cytometric analysis and fluorescent microscopy suggest that complex 1 induces caspase-dependent apoptosis followed with strong blockade of cell division in HCT116 cells. Since complex 1 showed ROS/RNS scavenging potential mentioned cytotoxicity was not connected with oxidative stress.",
publisher = "Elsevier",
journal = "Journal of Inorganic Biochemistry",
title = "Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies",
volume = "250",
doi = "10.1016/j.jinorgbio.2023.112399",
pages = "112399"
}

Kasalović, M. P., Jelača, S., Maksimović-Ivanić, D., Lađarević, J., Radovanović, L., Božić, B., Mijatović, S., Pantelić, N. Đ.,& Kaluđerović, G. N.. (2024). Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies. in Journal of Inorganic Biochemistry
Elsevier., 250, 112399.
https://doi.org/10.1016/j.jinorgbio.2023.112399

Kasalović MP, Jelača S, Maksimović-Ivanić D, Lađarević J, Radovanović L, Božić B, Mijatović S, Pantelić NĐ, Kaluđerović GN. Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies. in Journal of Inorganic Biochemistry. 2024;250:112399.
doi:10.1016/j.jinorgbio.2023.112399 .

Kasalović, Marijana P., Jelača, Sanja, Maksimović-Ivanić, Danijela, Lađarević, Jelena, Radovanović, Lidija, Božić, Bojan, Mijatović, Sanja, Pantelić, Nebojša Đ., Kaluđerović, Goran N., "Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies" in Journal of Inorganic Biochemistry, 250 (2024):112399,
https://doi.org/10.1016/j.jinorgbio.2023.112399 . .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Ukropina, Mirela
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
AU  - Ivanović, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5579
AB  - Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.
PB  - Elsevier GmbH.
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)
VL  - 249
DO  - 10.1016/j.aanat.2023.152097
SP  - 152097
ER  - 

@article{
author = "Ajduković, Maja and Ukropina, Mirela and Cvijanović, Milena and Vučić, Tijana and Ivanović, Ana",
year = "2023",
abstract = "Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.",
publisher = "Elsevier GmbH.",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)",
volume = "249",
doi = "10.1016/j.aanat.2023.152097",
pages = "152097"
}

Ajduković, M., Ukropina, M., Cvijanović, M., Vučić, T.,& Ivanović, A.. (2023). Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger
Elsevier GmbH.., 249, 152097.
https://doi.org/10.1016/j.aanat.2023.152097

Ajduković M, Ukropina M, Cvijanović M, Vučić T, Ivanović A. Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger. 2023;249:152097.
doi:10.1016/j.aanat.2023.152097 .

Ajduković, Maja, Ukropina, Mirela, Cvijanović, Milena, Vučić, Tijana, Ivanović, Ana, "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)" in Annals of Anatomy - Anatomischer Anzeiger, 249 (2023):152097,
https://doi.org/10.1016/j.aanat.2023.152097 . .

TY  - JOUR
AU  - Prijović, Mladen
AU  - Nikolić, Bogdan
AU  - Dragićević, Ivana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Giba, Zlatko
AU  - Jovanović, Vladan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6562
AB  - Plant protection with natural products is a new trend in environmentally friendly agriculture.
Nepeta rtanjensis Diklić et Milojević is an endemic and critically endangered plant species in Serbia.
We explored the phytotoxic potential of a water emulsion of Nepeta rtanjensis essential oil (NrEO)
with high amounts of trans,cis-nepetalactone on five weeds. The most sensitive was Stellaria media
(L.) Vill., as NrEO (from 0.013% to 0.1%) completely inhibited germination. Germination of
Amaranthus retroflexus L. and Artemisia vulgaris L. was completely inhibited at the highest applied
concentrations of NrEO (0.1%), while Ambrosia artemisiifolia L. germination was reduced to 48% at
the same concentration of NrEO. The most tolerant species was Cephalaria transsylvanica (L.) Schrad.
ex Roem. & Schult. as the final germination rate at the highest applied concentration of NrEO (0.1%)
was 81%, like the control (82%). To our knowledge, this is the first time the interaction of essential oils
on the germination and growth of A. vulgaris and C. transsylvanica is reported. The germination
dynamics of S. media in pots with soil were significantly inhibited when the soil was initially treated
with the highest applied concentration of the NrEO water emulsion (1%). Spraying S. media seedlings
with NrEO significantly inhibited growth parameters (shoot height, shoot fresh weight, and the number
of nodes) at the higher applied concentrations of NrEO (0.5% and 1%). Therefore, the water emulsion
of the essential oil of N. rtanjensis could be potentially developed for use in the control of invasive and
allergenic weeds.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide
DO  - 10.2298/ABS231107041P
ER  - 

@article{
author = "Prijović, Mladen and Nikolić, Bogdan and Dragićević, Ivana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Giba, Zlatko and Jovanović, Vladan",
year = "2023",
abstract = "Plant protection with natural products is a new trend in environmentally friendly agriculture.
Nepeta rtanjensis Diklić et Milojević is an endemic and critically endangered plant species in Serbia.
We explored the phytotoxic potential of a water emulsion of Nepeta rtanjensis essential oil (NrEO)
with high amounts of trans,cis-nepetalactone on five weeds. The most sensitive was Stellaria media
(L.) Vill., as NrEO (from 0.013% to 0.1%) completely inhibited germination. Germination of
Amaranthus retroflexus L. and Artemisia vulgaris L. was completely inhibited at the highest applied
concentrations of NrEO (0.1%), while Ambrosia artemisiifolia L. germination was reduced to 48% at
the same concentration of NrEO. The most tolerant species was Cephalaria transsylvanica (L.) Schrad.
ex Roem. & Schult. as the final germination rate at the highest applied concentration of NrEO (0.1%)
was 81%, like the control (82%). To our knowledge, this is the first time the interaction of essential oils
on the germination and growth of A. vulgaris and C. transsylvanica is reported. The germination
dynamics of S. media in pots with soil were significantly inhibited when the soil was initially treated
with the highest applied concentration of the NrEO water emulsion (1%). Spraying S. media seedlings
with NrEO significantly inhibited growth parameters (shoot height, shoot fresh weight, and the number
of nodes) at the higher applied concentrations of NrEO (0.5% and 1%). Therefore, the water emulsion
of the essential oil of N. rtanjensis could be potentially developed for use in the control of invasive and
allergenic weeds.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide",
doi = "10.2298/ABS231107041P"
}

Prijović, M., Nikolić, B., Dragićević, I., Nestorović Živković, J., Dmitrović, S., Giba, Z.,& Jovanović, V.. (2023). Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide. in Archives of Biological Sciences
Belgrade: Serbian Biological Society..
https://doi.org/10.2298/ABS231107041P

Prijović M, Nikolić B, Dragićević I, Nestorović Živković J, Dmitrović S, Giba Z, Jovanović V. Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide. in Archives of Biological Sciences. 2023;.
doi:10.2298/ABS231107041P .

Prijović, Mladen, Nikolić, Bogdan, Dragićević, Ivana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Giba, Zlatko, Jovanović, Vladan, "Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide" in Archives of Biological Sciences (2023),
https://doi.org/10.2298/ABS231107041P . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5818
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
DO  - 10.3389/fevo.2023.1148309
SP  - 1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
doi = "10.3389/fevo.2023.1148309",
pages = "1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Aleksić, Marija
AU  - Golić, Igor
AU  - Janković, Aleksandra
AU  - Čvoro, Aleksandra
AU  - Korać, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6529
AB  - We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.
PB  - London: Royal society publishing
T2  - Royal Society Open Science
T1  - ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats
VL  - 10
DO  - 10.1098/rsos.230109
SP  - 230109
ER  - 

@article{
author = "Aleksić, Marija and Golić, Igor and Janković, Aleksandra and Čvoro, Aleksandra and Korać, Aleksandra",
year = "2023",
abstract = "We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.",
publisher = "London: Royal society publishing",
journal = "Royal Society Open Science",
title = "ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats",
volume = "10",
doi = "10.1098/rsos.230109",
pages = "230109"
}

Aleksić, M., Golić, I., Janković, A., Čvoro, A.,& Korać, A.. (2023). ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats. in Royal Society Open Science
London: Royal society publishing., 10, 230109.
https://doi.org/10.1098/rsos.230109

Aleksić M, Golić I, Janković A, Čvoro A, Korać A. ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats. in Royal Society Open Science. 2023;10:230109.
doi:10.1098/rsos.230109 .

Aleksić, Marija, Golić, Igor, Janković, Aleksandra, Čvoro, Aleksandra, Korać, Aleksandra, "ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats" in Royal Society Open Science, 10 (2023):230109,
https://doi.org/10.1098/rsos.230109 . .

TY  - CONF
AU  - Savić, Tatjana
AU  - Pavković-Lučić, Sofija
AU  - Vujić, Vukica
AU  - Trajković, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6530
AB  - Drosophila melanogaster, kao vrsta sa kosmopolitskom distribucijom i generalista u ishrani,
predstavlja pogodan model sistem za izučavanje mnogih aspekata u oblasti evolucione
biologije, uključujući i komponente adaptivne vrednosti. Izbor mesta za polaganje jaja
predstavlja jednu od složenih osobina ponašanja ove vrste, koja je povezana sa povoljnim
uslovima za razviće potomstva.
U ovom radu je analiziran izbor supstrata za polaganje jaja 4-5 dana starih mušica D.
melanogaster, kao i sledeće komponente adaptivne vrednosti: brzina razvića, dinamika
izleganja i preživljavanje od jaja do adulta. Izbor mesta za polaganje jaja, kao i komponente
adaptivne vrednosti su testirane u laboratorijskim uslovima, ponudom različitih nutritivnih
podloga ženkama koje su odgajane na standardnom kukuruznom supstratu. U
eksperimentu je korišćeno 25 providnih plastičnih kutija, u kojima je postavljeno po pet
Petri posuda, ispunjenih standardnim kukuruznim supstratom, i supstratima koji su se
sastojali od paradajza, banane, šargarepe i jabuke. U svaku kutiju je tokom noći stavljeno po
25 jedinki oba pola, nakon čega su prebrojana jaja.
Rezultati ukazuju na postojanje značajnih razlika u broju položenih jaja na različitim
supstratima. Razviće je bilo najbrže kod mušica koje su kompletirale svoje razviće na
standardnoj kukuruznoj podlozi. Takođe, utvrđena je značajna razlika kako u dinamici
razvića, tako i u preživljavanju od jaja do adulta, između mušica koje su kompletirale
razviće na različitim supstratima.
Dobijeni rezultati pokazuju da su ženke polagale najmanji broj jaja na podlogama koje su
pravljene sa dodatkom jabuke i banane, na kojima ili nije bilo izleganja ili je fitnes bio
najniži.
PB  - Beograd: Entomološko društvo Srbije
C3  - Zbornik rezimea: 14. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2023 Sep 13-16; Novi Sad, Serbia
T1  - Izbor supstrata za polaganje jaja i komponente adaptivne vrednosti kod Drosophila melanogaster (Diptera: Drosophilidae)
SP  - 86
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6530
ER  - 

@conference{
author = "Savić, Tatjana and Pavković-Lučić, Sofija and Vujić, Vukica and Trajković, Jelena",
year = "2023",
abstract = "Drosophila melanogaster, kao vrsta sa kosmopolitskom distribucijom i generalista u ishrani,
predstavlja pogodan model sistem za izučavanje mnogih aspekata u oblasti evolucione
biologije, uključujući i komponente adaptivne vrednosti. Izbor mesta za polaganje jaja
predstavlja jednu od složenih osobina ponašanja ove vrste, koja je povezana sa povoljnim
uslovima za razviće potomstva.
U ovom radu je analiziran izbor supstrata za polaganje jaja 4-5 dana starih mušica D.
melanogaster, kao i sledeće komponente adaptivne vrednosti: brzina razvića, dinamika
izleganja i preživljavanje od jaja do adulta. Izbor mesta za polaganje jaja, kao i komponente
adaptivne vrednosti su testirane u laboratorijskim uslovima, ponudom različitih nutritivnih
podloga ženkama koje su odgajane na standardnom kukuruznom supstratu. U
eksperimentu je korišćeno 25 providnih plastičnih kutija, u kojima je postavljeno po pet
Petri posuda, ispunjenih standardnim kukuruznim supstratom, i supstratima koji su se
sastojali od paradajza, banane, šargarepe i jabuke. U svaku kutiju je tokom noći stavljeno po
25 jedinki oba pola, nakon čega su prebrojana jaja.
Rezultati ukazuju na postojanje značajnih razlika u broju položenih jaja na različitim
supstratima. Razviće je bilo najbrže kod mušica koje su kompletirale svoje razviće na
standardnoj kukuruznoj podlozi. Takođe, utvrđena je značajna razlika kako u dinamici
razvića, tako i u preživljavanju od jaja do adulta, između mušica koje su kompletirale
razviće na različitim supstratima.
Dobijeni rezultati pokazuju da su ženke polagale najmanji broj jaja na podlogama koje su
pravljene sa dodatkom jabuke i banane, na kojima ili nije bilo izleganja ili je fitnes bio
najniži.",
publisher = "Beograd: Entomološko društvo Srbije",
journal = "Zbornik rezimea: 14. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2023 Sep 13-16; Novi Sad, Serbia",
title = "Izbor supstrata za polaganje jaja i komponente adaptivne vrednosti kod Drosophila melanogaster (Diptera: Drosophilidae)",
pages = "86-87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6530"
}

Savić, T., Pavković-Lučić, S., Vujić, V.,& Trajković, J.. (2023). Izbor supstrata za polaganje jaja i komponente adaptivne vrednosti kod Drosophila melanogaster (Diptera: Drosophilidae). in Zbornik rezimea: 14. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2023 Sep 13-16; Novi Sad, Serbia
Beograd: Entomološko društvo Srbije., 86-87.
https://hdl.handle.net/21.15107/rcub_ibiss_6530

Savić T, Pavković-Lučić S, Vujić V, Trajković J. Izbor supstrata za polaganje jaja i komponente adaptivne vrednosti kod Drosophila melanogaster (Diptera: Drosophilidae). in Zbornik rezimea: 14. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2023 Sep 13-16; Novi Sad, Serbia. 2023;:86-87.
https://hdl.handle.net/21.15107/rcub_ibiss_6530 .

Savić, Tatjana, Pavković-Lučić, Sofija, Vujić, Vukica, Trajković, Jelena, "Izbor supstrata za polaganje jaja i komponente adaptivne vrednosti kod Drosophila melanogaster (Diptera: Drosophilidae)" in Zbornik rezimea: 14. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2023 Sep 13-16; Novi Sad, Serbia (2023):86-87,
https://hdl.handle.net/21.15107/rcub_ibiss_6530 .

TY  - JOUR
AU  - Miletić, Milica
AU  - Ivanov, Marija
AU  - Topalović, Aleksandra
AU  - Gavrilović, Milan
AU  - Gašić, Uroš
AU  - Janaćković, Peđa
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6465
AB  - Xanthium spinosum is a cosmopolitan annual herb used in traditional medicine worldwide. Although known from ethnobotanical studies, the species is scarcely investigated from the aspects of phytochemistry and biological activity. Therefore, the phenolic composition and biological activity of X. spinosum were examined. Plant specialised metabolites (phenolics) extracted from the roots, leaves and fruits with dichloromethane:methanol (1:1) were analysed by liquid chromatography mass spectrometry (LC-MS). In total 10 phenolic compounds were identified and quantified. Six compounds were common to all the extracts. Chlorogenic acid was the most abundant constituent in all the extracts (4.262 mg/g in the fruit extract, 0.820 mg/g in the leaf extract, and 0.540 mg/g in the root extract). The biological activity (antimicrobial and antibiofilm) of the extracts was tested against 12 microfungi and 12 bacterial strains by the microdilution method. All the extracts exhibited moderate antimicrobial and antibiofilm activity and inhibited the growth of most of the examined microorganisms. The obtained results indicate the potential role of the tested extracts in pharmacy and medicine.
PB  - Belgrade: Institute of Botany and Botanical Garden "Jevremovac"
T2  - Botanica Serbica
T1  - The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts
IS  - 1
VL  - 47
DO  - 10.2298/BOTSERB2301093M
SP  - 93
EP  - 101
ER  - 

@article{
author = "Miletić, Milica and Ivanov, Marija and Topalović, Aleksandra and Gavrilović, Milan and Gašić, Uroš and Janaćković, Peđa",
year = "2023",
abstract = "Xanthium spinosum is a cosmopolitan annual herb used in traditional medicine worldwide. Although known from ethnobotanical studies, the species is scarcely investigated from the aspects of phytochemistry and biological activity. Therefore, the phenolic composition and biological activity of X. spinosum were examined. Plant specialised metabolites (phenolics) extracted from the roots, leaves and fruits with dichloromethane:methanol (1:1) were analysed by liquid chromatography mass spectrometry (LC-MS). In total 10 phenolic compounds were identified and quantified. Six compounds were common to all the extracts. Chlorogenic acid was the most abundant constituent in all the extracts (4.262 mg/g in the fruit extract, 0.820 mg/g in the leaf extract, and 0.540 mg/g in the root extract). The biological activity (antimicrobial and antibiofilm) of the extracts was tested against 12 microfungi and 12 bacterial strains by the microdilution method. All the extracts exhibited moderate antimicrobial and antibiofilm activity and inhibited the growth of most of the examined microorganisms. The obtained results indicate the potential role of the tested extracts in pharmacy and medicine.",
publisher = "Belgrade: Institute of Botany and Botanical Garden "Jevremovac"",
journal = "Botanica Serbica",
title = "The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts",
number = "1",
volume = "47",
doi = "10.2298/BOTSERB2301093M",
pages = "93-101"
}

Miletić, M., Ivanov, M., Topalović, A., Gavrilović, M., Gašić, U.,& Janaćković, P.. (2023). The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden "Jevremovac"., 47(1), 93-101.
https://doi.org/10.2298/BOTSERB2301093M

Miletić M, Ivanov M, Topalović A, Gavrilović M, Gašić U, Janaćković P. The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts. in Botanica Serbica. 2023;47(1):93-101.
doi:10.2298/BOTSERB2301093M .

Miletić, Milica, Ivanov, Marija, Topalović, Aleksandra, Gavrilović, Milan, Gašić, Uroš, Janaćković, Peđa, "The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts" in Botanica Serbica, 47, no. 1 (2023):93-101,
https://doi.org/10.2298/BOTSERB2301093M . .

TY  - JOUR
AU  - Bjelica, Vukašin
AU  - Milićević, Ana
AU  - Bugarčić, Marko
AU  - Anđelković, Marko
PY  - 2023
UR  - https://biozoojournals.ro/nwjz/content/v19n2.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6454
PB  - Oradea, Romania: Herpetological Club of Oradea (HCO)
T2  - North-Western Journal of Zoology
T1  - Winter activity of the Caspian whipsnake (Dolichophis caspius, Gmelin, 1789) in Belgrade, Serbia
IS  - 2
VL  - 19
SP  - 212
EP  - 214
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6454
ER  - 

@article{
author = "Bjelica, Vukašin and Milićević, Ana and Bugarčić, Marko and Anđelković, Marko",
year = "2023",
publisher = "Oradea, Romania: Herpetological Club of Oradea (HCO)",
journal = "North-Western Journal of Zoology",
title = "Winter activity of the Caspian whipsnake (Dolichophis caspius, Gmelin, 1789) in Belgrade, Serbia",
number = "2",
volume = "19",
pages = "212-214",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6454"
}

Bjelica, V., Milićević, A., Bugarčić, M.,& Anđelković, M.. (2023). Winter activity of the Caspian whipsnake (Dolichophis caspius, Gmelin, 1789) in Belgrade, Serbia. in North-Western Journal of Zoology
Oradea, Romania: Herpetological Club of Oradea (HCO)., 19(2), 212-214.
https://hdl.handle.net/21.15107/rcub_ibiss_6454

Bjelica V, Milićević A, Bugarčić M, Anđelković M. Winter activity of the Caspian whipsnake (Dolichophis caspius, Gmelin, 1789) in Belgrade, Serbia. in North-Western Journal of Zoology. 2023;19(2):212-214.
https://hdl.handle.net/21.15107/rcub_ibiss_6454 .

Bjelica, Vukašin, Milićević, Ana, Bugarčić, Marko, Anđelković, Marko, "Winter activity of the Caspian whipsnake (Dolichophis caspius, Gmelin, 1789) in Belgrade, Serbia" in North-Western Journal of Zoology, 19, no. 2 (2023):212-214,
https://hdl.handle.net/21.15107/rcub_ibiss_6454 .

TY  - JOUR
AU  - Alimpić-Aradski, Ana
AU  - Olađe Pavlović, Mariana
AU  - Janošević, Dušica
AU  - Todorović, Slađana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Pljevljakušić, Dejan
AU  - Šavikin, Katarina
AU  - Marin, Petar D.
AU  - Giweli, Abdulhmid
AU  - Duletić-Laušević, Sonja
PY  - 2023
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/5152
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6445
AB  - Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.
PB  - Hoboken: John Wiley and Sons
T2  - Phytochemical Analysis
T1  - Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)
IS  - 6
VL  - 34
DO  - 10.1002/pca.3257
SP  - 661
EP  - 679
ER  - 

@article{
author = "Alimpić-Aradski, Ana and Olađe Pavlović, Mariana and Janošević, Dušica and Todorović, Slađana and Gašić, Uroš and Mišić, Danijela and Pljevljakušić, Dejan and Šavikin, Katarina and Marin, Petar D. and Giweli, Abdulhmid and Duletić-Laušević, Sonja",
year = "2023",
abstract = "Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Phytochemical Analysis",
title = "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)",
number = "6",
volume = "34",
doi = "10.1002/pca.3257",
pages = "661-679"
}

Alimpić-Aradski, A., Olađe Pavlović, M., Janošević, D., Todorović, S., Gašić, U., Mišić, D., Pljevljakušić, D., Šavikin, K., Marin, P. D., Giweli, A.,& Duletić-Laušević, S.. (2023). Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis
Hoboken: John Wiley and Sons., 34(6), 661-679.
https://doi.org/10.1002/pca.3257

Alimpić-Aradski A, Olađe Pavlović M, Janošević D, Todorović S, Gašić U, Mišić D, Pljevljakušić D, Šavikin K, Marin PD, Giweli A, Duletić-Laušević S. Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis. 2023;34(6):661-679.
doi:10.1002/pca.3257 .

Alimpić-Aradski, Ana, Olađe Pavlović, Mariana, Janošević, Dušica, Todorović, Slađana, Gašić, Uroš, Mišić, Danijela, Pljevljakušić, Dejan, Šavikin, Katarina, Marin, Petar D., Giweli, Abdulhmid, Duletić-Laušević, Sonja, "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)" in Phytochemical Analysis, 34, no. 6 (2023):661-679,
https://doi.org/10.1002/pca.3257 . .

TY  - CONF
AU  - Pajić, Tanja
AU  - Todorović, Nataša
AU  - Živić, Miroslav
AU  - Nikolić, Stanko N
AU  - Rabasović, Mihailo D
AU  - Clayton, Andrew HA
AU  - Krmpot, Aleksandar J
PY  - 2023
UR  - https://www.eventclass.org/contxt_emim2023/online-program/session?s=PW36#e609
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6288
AB  - Introduction
Studies of lipid droplet (LD) physiology in fungi are still in their infancy but their quantitation
has relevance to issues in biomedicine, agriculture and industrial waste. Third Harmonic
Generation (THG) microscopy is non-invasive, produces inherently confocal images and
doesn’t require fixation or external labeling, which make it suitable for in vivo LD imaging [1,
2]. We present in vivo and label-free imaging of LD in individual fungal cells by THG
microscopy to assess the effects of nitrogen starvation. The LD quantification was performed
by two image analysis techniques.
Methods
THG microscopy was applied for the first time to a filamentous fungus and our choice was the
oleaginous fungus Phycomyces blakesleeanus. To observe the changes in LD number, the
22h old hyphae culture was divided into control and nitrogen starved groups (N-starved). A
home built nonlienar microscope with Yb:KGW laser at 1040 nm (200 fs pulses, 83 MHz
repetition rate) was used for THG imaging of live unstained hyphae [3]. THG signal was
detected by PMT in the transmission arm after passing through a Hoya glass UV filter with the
peak at 340 nm. 2D THG images of LDs (Fig. 1a) were analyzed by Image Correlation
Spectroscopy (ICS) measuring spatially-correlated fluctuations [4] and software particle
counting – Particle Size Analysis (PSA).
Results/Discussion
The small volume of hyphae suspension was placed between two coverslips of 170 μm
thickness in order to meet the criteria for the best numerical aperture of the objective lens and
for better transmission of THG signal. The high resolution of the microscopic system, the
hyphae thickness (ca 10 μm) and medium transparency made it possible for the whole
hyphae to be optically sectioned and a 3D model to be reconstructed (Fig. 1b and video).
Since ICS was primarily developed for fluorescent images and was not used to analyze THG
images, we have tested it by comparing the results to the PSA. Nitrogen starvation as
expected [5] increased LD number compared to control which was confirmed by both methods and obtained results are in good agreement. The overall increase of LDs during
growth without available nitrogen is found to be between 3 and 4.5 h time point, followed with
the loss of population of larger-than-average LDs during prolonged starvation.
Conclusions
THG microscopy is suitable for imaging and quantification of changes in lipid droplet number,
brought upon by complete removal of nitrogen, from such low density/diameter baseline. In
addition, we demonstrate that the ICA is suitable for THG images, although it is primarily
developed and have been mostly used for fluorescence signals so far.
PB  - European Society for Molecular Imaging
C3  - European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria
T1  - Third harmonic generation imaging of live fungal cells – quantifying lipid droplets dynamics during nitrogen starvation
SP  - 1093
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6288
ER  - 

@conference{
author = "Pajić, Tanja and Todorović, Nataša and Živić, Miroslav and Nikolić, Stanko N and Rabasović, Mihailo D and Clayton, Andrew HA and Krmpot, Aleksandar J",
year = "2023",
abstract = "Introduction
Studies of lipid droplet (LD) physiology in fungi are still in their infancy but their quantitation
has relevance to issues in biomedicine, agriculture and industrial waste. Third Harmonic
Generation (THG) microscopy is non-invasive, produces inherently confocal images and
doesn’t require fixation or external labeling, which make it suitable for in vivo LD imaging [1,
2]. We present in vivo and label-free imaging of LD in individual fungal cells by THG
microscopy to assess the effects of nitrogen starvation. The LD quantification was performed
by two image analysis techniques.
Methods
THG microscopy was applied for the first time to a filamentous fungus and our choice was the
oleaginous fungus Phycomyces blakesleeanus. To observe the changes in LD number, the
22h old hyphae culture was divided into control and nitrogen starved groups (N-starved). A
home built nonlienar microscope with Yb:KGW laser at 1040 nm (200 fs pulses, 83 MHz
repetition rate) was used for THG imaging of live unstained hyphae [3]. THG signal was
detected by PMT in the transmission arm after passing through a Hoya glass UV filter with the
peak at 340 nm. 2D THG images of LDs (Fig. 1a) were analyzed by Image Correlation
Spectroscopy (ICS) measuring spatially-correlated fluctuations [4] and software particle
counting – Particle Size Analysis (PSA).
Results/Discussion
The small volume of hyphae suspension was placed between two coverslips of 170 μm
thickness in order to meet the criteria for the best numerical aperture of the objective lens and
for better transmission of THG signal. The high resolution of the microscopic system, the
hyphae thickness (ca 10 μm) and medium transparency made it possible for the whole
hyphae to be optically sectioned and a 3D model to be reconstructed (Fig. 1b and video).
Since ICS was primarily developed for fluorescent images and was not used to analyze THG
images, we have tested it by comparing the results to the PSA. Nitrogen starvation as
expected [5] increased LD number compared to control which was confirmed by both methods and obtained results are in good agreement. The overall increase of LDs during
growth without available nitrogen is found to be between 3 and 4.5 h time point, followed with
the loss of population of larger-than-average LDs during prolonged starvation.
Conclusions
THG microscopy is suitable for imaging and quantification of changes in lipid droplet number,
brought upon by complete removal of nitrogen, from such low density/diameter baseline. In
addition, we demonstrate that the ICA is suitable for THG images, although it is primarily
developed and have been mostly used for fluorescence signals so far.",
publisher = "European Society for Molecular Imaging",
journal = "European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria",
title = "Third harmonic generation imaging of live fungal cells – quantifying lipid droplets dynamics during nitrogen starvation",
pages = "1093",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6288"
}

Pajić, T., Todorović, N., Živić, M., Nikolić, S. N., Rabasović, M. D., Clayton, A. H.,& Krmpot, A. J.. (2023). Third harmonic generation imaging of live fungal cells – quantifying lipid droplets dynamics during nitrogen starvation. in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria
European Society for Molecular Imaging., 1093.
https://hdl.handle.net/21.15107/rcub_ibiss_6288

Pajić T, Todorović N, Živić M, Nikolić SN, Rabasović MD, Clayton AH, Krmpot AJ. Third harmonic generation imaging of live fungal cells – quantifying lipid droplets dynamics during nitrogen starvation. in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria. 2023;:1093.
https://hdl.handle.net/21.15107/rcub_ibiss_6288 .

Pajić, Tanja, Todorović, Nataša, Živić, Miroslav, Nikolić, Stanko N, Rabasović, Mihailo D, Clayton, Andrew HA, Krmpot, Aleksandar J, "Third harmonic generation imaging of live fungal cells – quantifying lipid droplets dynamics during nitrogen starvation" in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria (2023):1093,
https://hdl.handle.net/21.15107/rcub_ibiss_6288 .

TY  - JOUR
AU  - Mileski, Ksenija S.
AU  - Ćirić, Ana
AU  - Gašić, Uroš
AU  - Žarković, Lazar D.
AU  - Krivošej, Zoran Đ.
AU  - Džamić, Ana M.
PY  - 2023
UR  - https://www.acgpubs.org/doc/20221125121922364-RNP-2207-2528.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5345
AB  - The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.
T2  - Records of Natural Products
T1  - Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia
IS  - 3
VL  - 17
DO  - 10.25135/rnp.364.2207.2528
SP  - 453
EP  - 475
ER  - 

@article{
author = "Mileski, Ksenija S. and Ćirić, Ana and Gašić, Uroš and Žarković, Lazar D. and Krivošej, Zoran Đ. and Džamić, Ana M.",
year = "2023",
abstract = "The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.",
journal = "Records of Natural Products",
title = "Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia",
number = "3",
volume = "17",
doi = "10.25135/rnp.364.2207.2528",
pages = "453-475"
}

Mileski, K. S., Ćirić, A., Gašić, U., Žarković, L. D., Krivošej, Z. Đ.,& Džamić, A. M.. (2023). Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia. in Records of Natural Products, 17(3), 453-475.
https://doi.org/10.25135/rnp.364.2207.2528

Mileski KS, Ćirić A, Gašić U, Žarković LD, Krivošej ZĐ, Džamić AM. Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia. in Records of Natural Products. 2023;17(3):453-475.
doi:10.25135/rnp.364.2207.2528 .

Mileski, Ksenija S., Ćirić, Ana, Gašić, Uroš, Žarković, Lazar D., Krivošej, Zoran Đ., Džamić, Ana M., "Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia" in Records of Natural Products, 17, no. 3 (2023):453-475,
https://doi.org/10.25135/rnp.364.2207.2528 . .

TY  - CONF
AU  - Stevanović, Katarina
AU  - Pajić, Tanja
AU  - Krmpot, Aleksandar
AU  - Rabasović, Mihailo
AU  - Žižić, Milan
AU  - Živić, Miroslav
AU  - Todorović, Nataša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6246
AB  - The success of patch-clamp giga-ohm seal formation on filamentous fungi Phycomyces blakesleeanus protoplasts was investigated to evaluate their usefulness in ion channel studies on filamentous fungi. Protoplasts were obtained by laser-mediated nanosurgery of the cell wall stained with Calcofluor White. To enable a successful seal formation, it is critical to prevent cell wall regeneration. Since wall integrity responses in fungi involve kinase-dependent pathways, we aimed to sufficiently reduce intracellular ATP availability using the respiratory inhibitor azide. The effect of azide on phosphate metabolites of Phycomyces blakesleeanus, as determined by 31P NMR spectroscopy, was a reduction in intracellular ATP accompanied by a decrease in long chain polyphosphates. Subsequently, all seal formation measurements were performed in the presence of azide, and protoplast viability was confirmed by cytoplasmic streaming. The success of seal formation depends on the size of the protoplasts, as larger protoplasts are more prone to successful seal formation. It was also found that the laser power used in nanosurgery could influence the success of seal formation, as higher values were associated with a lower success rate. The protoplasts released by laser nanosurgery produced by our newly developed method are a good model system for patch clamp on filamentous fungal membranes because their plasma membrane can easily form high-quality seals with the patch pipette.
PB  - Kragujevac: Institute for Information Technologies, University of Belgrade
C3  - Book of Proceedings: 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023; 2023 Sep 28-29; Kragujevac, Serbia
T1  - Patch clamp pipette giga seal forming success on the nanosurgery-obtained filamentous fungi protoplasts
DO  - 10.46793/ICCBI23.221S
SP  - 221
EP  - 224
ER  - 

@conference{
author = "Stevanović, Katarina and Pajić, Tanja and Krmpot, Aleksandar and Rabasović, Mihailo and Žižić, Milan and Živić, Miroslav and Todorović, Nataša",
year = "2023",
abstract = "The success of patch-clamp giga-ohm seal formation on filamentous fungi Phycomyces blakesleeanus protoplasts was investigated to evaluate their usefulness in ion channel studies on filamentous fungi. Protoplasts were obtained by laser-mediated nanosurgery of the cell wall stained with Calcofluor White. To enable a successful seal formation, it is critical to prevent cell wall regeneration. Since wall integrity responses in fungi involve kinase-dependent pathways, we aimed to sufficiently reduce intracellular ATP availability using the respiratory inhibitor azide. The effect of azide on phosphate metabolites of Phycomyces blakesleeanus, as determined by 31P NMR spectroscopy, was a reduction in intracellular ATP accompanied by a decrease in long chain polyphosphates. Subsequently, all seal formation measurements were performed in the presence of azide, and protoplast viability was confirmed by cytoplasmic streaming. The success of seal formation depends on the size of the protoplasts, as larger protoplasts are more prone to successful seal formation. It was also found that the laser power used in nanosurgery could influence the success of seal formation, as higher values were associated with a lower success rate. The protoplasts released by laser nanosurgery produced by our newly developed method are a good model system for patch clamp on filamentous fungal membranes because their plasma membrane can easily form high-quality seals with the patch pipette.",
publisher = "Kragujevac: Institute for Information Technologies, University of Belgrade",
journal = "Book of Proceedings: 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023; 2023 Sep 28-29; Kragujevac, Serbia",
title = "Patch clamp pipette giga seal forming success on the nanosurgery-obtained filamentous fungi protoplasts",
doi = "10.46793/ICCBI23.221S",
pages = "221-224"
}

Stevanović, K., Pajić, T., Krmpot, A., Rabasović, M., Žižić, M., Živić, M.,& Todorović, N.. (2023). Patch clamp pipette giga seal forming success on the nanosurgery-obtained filamentous fungi protoplasts. in Book of Proceedings: 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023; 2023 Sep 28-29; Kragujevac, Serbia
Kragujevac: Institute for Information Technologies, University of Belgrade., 221-224.
https://doi.org/10.46793/ICCBI23.221S

Stevanović K, Pajić T, Krmpot A, Rabasović M, Žižić M, Živić M, Todorović N. Patch clamp pipette giga seal forming success on the nanosurgery-obtained filamentous fungi protoplasts. in Book of Proceedings: 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023; 2023 Sep 28-29; Kragujevac, Serbia. 2023;:221-224.
doi:10.46793/ICCBI23.221S .

Stevanović, Katarina, Pajić, Tanja, Krmpot, Aleksandar, Rabasović, Mihailo, Žižić, Milan, Živić, Miroslav, Todorović, Nataša, "Patch clamp pipette giga seal forming success on the nanosurgery-obtained filamentous fungi protoplasts" in Book of Proceedings: 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023; 2023 Sep 28-29; Kragujevac, Serbia (2023):221-224,
https://doi.org/10.46793/ICCBI23.221S . .

TY  - CONF
AU  - Vitkovac, Aleksandra
AU  - Pajić, Tanja
AU  - Bukumira, Marta
AU  - Stanić, Marina
AU  - Rabasović, Mihailo
AU  - Todorović, Nataša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6247
AB  - Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.
PB  - Kragujevac: Institute for Information Technologies, University of Belgrade
C3  - Book of Proceedings: 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023; 2023 Sep 28-29; Kragujevac, Serbia
T1  - Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology
DO  - 10.46793/ICCBI23.334V
SP  - 334
EP  - 337
ER  - 

@conference{
author = "Vitkovac, Aleksandra and Pajić, Tanja and Bukumira, Marta and Stanić, Marina and Rabasović, Mihailo and Todorović, Nataša",
year = "2023",
abstract = "Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.",
publisher = "Kragujevac: Institute for Information Technologies, University of Belgrade",
journal = "Book of Proceedings: 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023; 2023 Sep 28-29; Kragujevac, Serbia",
title = "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology",
doi = "10.46793/ICCBI23.334V",
pages = "334-337"
}

Vitkovac, A., Pajić, T., Bukumira, M., Stanić, M., Rabasović, M.,& Todorović, N.. (2023). Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in Book of Proceedings: 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023; 2023 Sep 28-29; Kragujevac, Serbia
Kragujevac: Institute for Information Technologies, University of Belgrade., 334-337.
https://doi.org/10.46793/ICCBI23.334V

Vitkovac A, Pajić T, Bukumira M, Stanić M, Rabasović M, Todorović N. Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in Book of Proceedings: 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023; 2023 Sep 28-29; Kragujevac, Serbia. 2023;:334-337.
doi:10.46793/ICCBI23.334V .

Vitkovac, Aleksandra, Pajić, Tanja, Bukumira, Marta, Stanić, Marina, Rabasović, Mihailo, Todorović, Nataša, "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology" in Book of Proceedings: 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023; 2023 Sep 28-29; Kragujevac, Serbia (2023):334-337,
https://doi.org/10.46793/ICCBI23.334V . .

TY  - JOUR
AU  - Rammou, Dimitra-Lida
AU  - Karaiskou, Nikoleta
AU  - Minoudi, Styliani
AU  - Kazilas, Christos
AU  - Moulistanos, Aristotelis
AU  - Gkagkavouzis, Konstantinos
AU  - Ćirović, Duško
AU  - Nikolić, Tijana
AU  - Ćosić, Nada
AU  - Youlatos, Dionisios
AU  - Triantafyllidis, Alexandros
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6421
AB  - The Balkan Peninsula acted as a refugium for many steppic species during the Pleistocene, resulting in a high genetic variability, which, in some cases, presents a northward decreasing latitudinal gradient. In the present study, we investigate the genetic diversity of the European ground squirrel (Spermophilus citellus) in its Balkan distribution, sampled comprehensively across its entire distribution in Greece (133 samples from 21 localities). These samples are compared with others from Serbia (54 samples from eight localities) and the available published data from its range in Europe (124 sequences from GenBank). Our results show that the genetic diversity of the Greek and Serbian populations is not correlated with a latitudinal gradient. However, when the entire European range is considered, the model that best fits the genetic variation of the species is based on the ‘centre–periphery’ hypothesis. The founder (centre) populations of the species were most probably situated in south-eastern Bulgaria and European Turkey and underwent rapid expansion post-glacially, during the Holocene, both northwards (Central Europe) and southwards (Greece), as revealed by the decreased genetic diversity and phylogeographical inference analysis in these marginal regions. These results indicate a particular phylogeographical history of a European species and set a baseline for conservation management in its southern populations.
PB  - Oxford University Press
T2  - Biological Journal of the Linnean Society
T1  - Phylogeography of the European ground squirrel, Spermophilus citellus (Rodentia: Sciuridae), in the Balkans
IS  - 2
VL  - 139
DO  - 10.1093/biolinnean/blad021
SP  - 158
EP  - 172
ER  - 

@article{
author = "Rammou, Dimitra-Lida and Karaiskou, Nikoleta and Minoudi, Styliani and Kazilas, Christos and Moulistanos, Aristotelis and Gkagkavouzis, Konstantinos and Ćirović, Duško and Nikolić, Tijana and Ćosić, Nada and Youlatos, Dionisios and Triantafyllidis, Alexandros",
year = "2023",
abstract = "The Balkan Peninsula acted as a refugium for many steppic species during the Pleistocene, resulting in a high genetic variability, which, in some cases, presents a northward decreasing latitudinal gradient. In the present study, we investigate the genetic diversity of the European ground squirrel (Spermophilus citellus) in its Balkan distribution, sampled comprehensively across its entire distribution in Greece (133 samples from 21 localities). These samples are compared with others from Serbia (54 samples from eight localities) and the available published data from its range in Europe (124 sequences from GenBank). Our results show that the genetic diversity of the Greek and Serbian populations is not correlated with a latitudinal gradient. However, when the entire European range is considered, the model that best fits the genetic variation of the species is based on the ‘centre–periphery’ hypothesis. The founder (centre) populations of the species were most probably situated in south-eastern Bulgaria and European Turkey and underwent rapid expansion post-glacially, during the Holocene, both northwards (Central Europe) and southwards (Greece), as revealed by the decreased genetic diversity and phylogeographical inference analysis in these marginal regions. These results indicate a particular phylogeographical history of a European species and set a baseline for conservation management in its southern populations.",
publisher = "Oxford University Press",
journal = "Biological Journal of the Linnean Society",
title = "Phylogeography of the European ground squirrel, Spermophilus citellus (Rodentia: Sciuridae), in the Balkans",
number = "2",
volume = "139",
doi = "10.1093/biolinnean/blad021",
pages = "158-172"
}

Rammou, D., Karaiskou, N., Minoudi, S., Kazilas, C., Moulistanos, A., Gkagkavouzis, K., Ćirović, D., Nikolić, T., Ćosić, N., Youlatos, D.,& Triantafyllidis, A.. (2023). Phylogeography of the European ground squirrel, Spermophilus citellus (Rodentia: Sciuridae), in the Balkans. in Biological Journal of the Linnean Society
Oxford University Press., 139(2), 158-172.
https://doi.org/10.1093/biolinnean/blad021

Rammou D, Karaiskou N, Minoudi S, Kazilas C, Moulistanos A, Gkagkavouzis K, Ćirović D, Nikolić T, Ćosić N, Youlatos D, Triantafyllidis A. Phylogeography of the European ground squirrel, Spermophilus citellus (Rodentia: Sciuridae), in the Balkans. in Biological Journal of the Linnean Society. 2023;139(2):158-172.
doi:10.1093/biolinnean/blad021 .

Rammou, Dimitra-Lida, Karaiskou, Nikoleta, Minoudi, Styliani, Kazilas, Christos, Moulistanos, Aristotelis, Gkagkavouzis, Konstantinos, Ćirović, Duško, Nikolić, Tijana, Ćosić, Nada, Youlatos, Dionisios, Triantafyllidis, Alexandros, "Phylogeography of the European ground squirrel, Spermophilus citellus (Rodentia: Sciuridae), in the Balkans" in Biological Journal of the Linnean Society, 139, no. 2 (2023):158-172,
https://doi.org/10.1093/biolinnean/blad021 . .

TY  - CHAP
AU  - Raspor, Martin
AU  - Berić, Tanja
AU  - Ćosić, Tatjana
AU  - Stanković, Slaviša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6400
AB  - The rhizosphere is a microhabitat around plant roots that is actively created and regulated by plants, while having a major impact on plant life itself. Plants constantly enrich the rhizosphere with organic matter from their rhizodepositions and root exudates, shaping the chemical and microbiological composition of the space surrounding their roots. In this chapter, we discuss the interactions between plants and other organisms through the processes in the rhizosphere. Plant-associated microorganisms, such as plant growth-promoting bacteria and mycorrhizal fungi, play important roles in enhancing the survival of plants, through increasing the availability of nutrients from the soil to plants, degrading and immobilizing toxic compounds, alleviating the effects of abiotic stress, acting as biocontrol agents, protecting the plants from pathogens, and bolstering the efficiency of plant responses to biotic and abiotic stress. Moreover, plants use the rhizosphere as a battlefield for mutual competition by releasing allelochemicals that are detrimental to other plant species, and to harmful soil nematodes and insects. Similarly to land plants, aquatic plants also create a rhizosphere around their roots, with important implications for rice cultivation, methane emissions from wetlands, denitrification of aquatic habitats, and wastewater treatment through constructed wetlands. We emphasize how the existing and potential rhizosphere engineering approaches take advantage of the plant-associated microorganisms and allelopathic interactions between plants to improve the health and yield of agricultural crops, and help preserve the natural environment.
PB  - Cham: Springer
T2  - New Frontiers in Plant-Environment Interactions: Innovative Technologies and Developments
T1  - The Rhizosphere–A Hub of Emerging Importance for Plant Biotic Interactions
DO  - 10.1007/978-3-031-43729-8_11
SP  - 289
EP  - 327
ER  - 

@inbook{
author = "Raspor, Martin and Berić, Tanja and Ćosić, Tatjana and Stanković, Slaviša",
year = "2023",
abstract = "The rhizosphere is a microhabitat around plant roots that is actively created and regulated by plants, while having a major impact on plant life itself. Plants constantly enrich the rhizosphere with organic matter from their rhizodepositions and root exudates, shaping the chemical and microbiological composition of the space surrounding their roots. In this chapter, we discuss the interactions between plants and other organisms through the processes in the rhizosphere. Plant-associated microorganisms, such as plant growth-promoting bacteria and mycorrhizal fungi, play important roles in enhancing the survival of plants, through increasing the availability of nutrients from the soil to plants, degrading and immobilizing toxic compounds, alleviating the effects of abiotic stress, acting as biocontrol agents, protecting the plants from pathogens, and bolstering the efficiency of plant responses to biotic and abiotic stress. Moreover, plants use the rhizosphere as a battlefield for mutual competition by releasing allelochemicals that are detrimental to other plant species, and to harmful soil nematodes and insects. Similarly to land plants, aquatic plants also create a rhizosphere around their roots, with important implications for rice cultivation, methane emissions from wetlands, denitrification of aquatic habitats, and wastewater treatment through constructed wetlands. We emphasize how the existing and potential rhizosphere engineering approaches take advantage of the plant-associated microorganisms and allelopathic interactions between plants to improve the health and yield of agricultural crops, and help preserve the natural environment.",
publisher = "Cham: Springer",
journal = "New Frontiers in Plant-Environment Interactions: Innovative Technologies and Developments",
booktitle = "The Rhizosphere–A Hub of Emerging Importance for Plant Biotic Interactions",
doi = "10.1007/978-3-031-43729-8_11",
pages = "289-327"
}

Raspor, M., Berić, T., Ćosić, T.,& Stanković, S.. (2023). The Rhizosphere–A Hub of Emerging Importance for Plant Biotic Interactions. in New Frontiers in Plant-Environment Interactions: Innovative Technologies and Developments
Cham: Springer., 289-327.
https://doi.org/10.1007/978-3-031-43729-8_11

Raspor M, Berić T, Ćosić T, Stanković S. The Rhizosphere–A Hub of Emerging Importance for Plant Biotic Interactions. in New Frontiers in Plant-Environment Interactions: Innovative Technologies and Developments. 2023;:289-327.
doi:10.1007/978-3-031-43729-8_11 .

Raspor, Martin, Berić, Tanja, Ćosić, Tatjana, Stanković, Slaviša, "The Rhizosphere–A Hub of Emerging Importance for Plant Biotic Interactions" in New Frontiers in Plant-Environment Interactions: Innovative Technologies and Developments (2023):289-327,
https://doi.org/10.1007/978-3-031-43729-8_11 . .

TY  - JOUR
AU  - Jojić, Vida
AU  - Bajić, Aleksandar
AU  - Barišić Klisarić, Nataša
AU  - Bugarski-Stanojević, Vanja
AU  - Snoj, Aleš
AU  - Miljanović, Branko
AU  - Askeyev, Oleg
AU  - Askeyev, Igor
AU  - Marić, Saša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6390
AB  - This is a preliminary and exploratory study of cranial variation in European populations of grayling. We
investigated the correspondence between size/shape variation of the dorsal (dc), ventral (vc) and occipital
(oc) cranium and phylogenetic relationships (inferred from mitochondrial control region – mtDNA cr
and microsatellite dna data) of six grayling populations: three from Balkan phylogenetic clade and two
from Caspian phylogenetic clade of the European grayling Thymallus thymallus and one population of
the Adriatic grayling Thymallus aeliani, which until recently was considered the Adriatic phylogenetic
clade of T. thymallus. Significant size and shape differences were found between populations in all three
cranial views. However, significant size-related shape variation (allometry) was found for dc and vc, but
not for oc. The size variation of each cranial view does not contain phylogenetic signal, but size variation
of oc is consistent with genetic variation inferred from microsatellite dna. Regarding shape variation, a
significant phylogenetic signal was detected only for oc, and only the shape variation of oc is consistent
with the genetic variation inferred from the mtDNA cr. Moreover, the Adriatic grayling T. aeliani (Soča
population) was clearly separated from the three T. thymallus populations of the Balkan phylogenetic
clade and the two T. thymallus populations of the Caspian phylogenetic clade only at the level of oc. Thus,
our results suggest that different cranial regions differ in allometry, reflect phylo(genetic) relationships
differently, and exhibit differences in ecophenotypic plasticity, with oc seeming best suited to represent
the phylogenetic relationships of the grayling populations studied.
PB  - Leiden: Brill
T2  - Contributions to Zoology
T1  - Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae)
IS  - 5
VL  - 92
DO  - 10.1163/18759866-bja10051
SP  - 510
EP  - 532
ER  - 

@article{
author = "Jojić, Vida and Bajić, Aleksandar and Barišić Klisarić, Nataša and Bugarski-Stanojević, Vanja and Snoj, Aleš and Miljanović, Branko and Askeyev, Oleg and Askeyev, Igor and Marić, Saša",
year = "2023",
abstract = "This is a preliminary and exploratory study of cranial variation in European populations of grayling. We
investigated the correspondence between size/shape variation of the dorsal (dc), ventral (vc) and occipital
(oc) cranium and phylogenetic relationships (inferred from mitochondrial control region – mtDNA cr
and microsatellite dna data) of six grayling populations: three from Balkan phylogenetic clade and two
from Caspian phylogenetic clade of the European grayling Thymallus thymallus and one population of
the Adriatic grayling Thymallus aeliani, which until recently was considered the Adriatic phylogenetic
clade of T. thymallus. Significant size and shape differences were found between populations in all three
cranial views. However, significant size-related shape variation (allometry) was found for dc and vc, but
not for oc. The size variation of each cranial view does not contain phylogenetic signal, but size variation
of oc is consistent with genetic variation inferred from microsatellite dna. Regarding shape variation, a
significant phylogenetic signal was detected only for oc, and only the shape variation of oc is consistent
with the genetic variation inferred from the mtDNA cr. Moreover, the Adriatic grayling T. aeliani (Soča
population) was clearly separated from the three T. thymallus populations of the Balkan phylogenetic
clade and the two T. thymallus populations of the Caspian phylogenetic clade only at the level of oc. Thus,
our results suggest that different cranial regions differ in allometry, reflect phylo(genetic) relationships
differently, and exhibit differences in ecophenotypic plasticity, with oc seeming best suited to represent
the phylogenetic relationships of the grayling populations studied.",
publisher = "Leiden: Brill",
journal = "Contributions to Zoology",
title = "Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae)",
number = "5",
volume = "92",
doi = "10.1163/18759866-bja10051",
pages = "510-532"
}

Jojić, V., Bajić, A., Barišić Klisarić, N., Bugarski-Stanojević, V., Snoj, A., Miljanović, B., Askeyev, O., Askeyev, I.,& Marić, S.. (2023). Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae). in Contributions to Zoology
Leiden: Brill., 92(5), 510-532.
https://doi.org/10.1163/18759866-bja10051

Jojić V, Bajić A, Barišić Klisarić N, Bugarski-Stanojević V, Snoj A, Miljanović B, Askeyev O, Askeyev I, Marić S. Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae). in Contributions to Zoology. 2023;92(5):510-532.
doi:10.1163/18759866-bja10051 .

Jojić, Vida, Bajić, Aleksandar, Barišić Klisarić, Nataša, Bugarski-Stanojević, Vanja, Snoj, Aleš, Miljanović, Branko, Askeyev, Oleg, Askeyev, Igor, Marić, Saša, "Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae)" in Contributions to Zoology, 92, no. 5 (2023):510-532,
https://doi.org/10.1163/18759866-bja10051 . .

TY  - CONF
AU  - Pajić, Tanja
AU  - Kozakijević, Suzana
AU  - Krmpot, Aleksandar J.
AU  - Živić, Miroslav
AU  - Todorović, Nataša
AU  - Rabasović, Mihailo D.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6249
AB  - Mitochondrial function, and consequently cellular metabolic status and fitness of a cell, is tightly linked
to the dynamic changes of mitochondrial morphology, including mitochondrial fusion, fission and
mitophagy [1]. Lipid droplets (LDs) can be in close contact with mitochondria, and accumulate
autophagy or mitophagy generated material during the reparatory processes [2]. The effect of increased
ambient temperature on mitochondrial morphology and LDs density in living cells of the filamentous
fungus Phycomyces blakesleeanus was investigated. For in vivo imaging of mitochondria and LDs
multiphoton microscopy was used. Multiphoton microscopy enables 3D imaging in high resolution and
reduced photodamage and photobleaching of the sample using IR ultrafast pulsed lasers. Mitochondria
were stained with the vital dye Rhodamine123 (Rh123) which enters these organelles based on their
membrane potential - mitochondria must be healthy/active to stain. A wavelength of 800 nm from Ti:Sa
laser (160 fs pulse duration, 76 MHz repetition rate) was used for two-photon excitation of Rh123. The
laser beam was focused by the Zeiss Plan Neofluar 40x1.3 objective lens and the signal was detected
through a bandpass interference filter MF530/43 (ThorLabs, USA). For LDs staining a Nile Red dye
was used and excited by Yb: KGW laser at 1040 nm [3]. Six morphological types of mitochondria were
observed in the hyphae of this fungus: intermediate type - normal, intermediate with small semicircular
tubules, tubular, elongated tubular, fragmented (small ellipsoid tubule) and fragmented with exclusively
spheroid-shaped mitochondria. Changes in mitochondrial morphology were induced by a small
temperature change. A 3°C increase in ambient temperature, from 22°C, had a dramatic effect on
mitochondrial morphology, inducing the appearance of a predominantly tubular mitochondrial
morphology. The total area percentage of mitochondria showed an increasing trend when grown at
25°C. Increasing the ambient temperature to 25°C induced a statistically significant increase in the
percentage of hyphal area occupied by LDs from 2.9 ± 1.6 to 4.7 ± 2.2 (mean value and SD in percentage
of hyphal area). The observed response to the small temperature increase points to the physiological
adaptation of hyphal metabolism.
PB  - Belgrade: Vinča Institute of Nuclear Sciences – National Institute of the Republic of Serbia, University of Belgrade
C3  - Book of abstracts: 9th International School and Conference on Photonics PHOTONICA 2023 with joint events: Understanding interaction light - biological surfaces possibility for new electronic materials and devices and Biological and bioinspired structures for multispectral surveillance and Quantum sensing integration within microfluidic Lab-on-a Chips for biomedical applications; 2023 Aug 28 - Sep 01; Belgrade, Serbia
T1  - In vivo multiphoton imaging of a filamentous fungus Phycomyces blakesleeanus: the effect of small ambient temperature increase on mitochondrial morphology and lipid droplets density
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6249
ER  - 

@conference{
author = "Pajić, Tanja and Kozakijević, Suzana and Krmpot, Aleksandar J. and Živić, Miroslav and Todorović, Nataša and Rabasović, Mihailo D.",
year = "2023",
abstract = "Mitochondrial function, and consequently cellular metabolic status and fitness of a cell, is tightly linked
to the dynamic changes of mitochondrial morphology, including mitochondrial fusion, fission and
mitophagy [1]. Lipid droplets (LDs) can be in close contact with mitochondria, and accumulate
autophagy or mitophagy generated material during the reparatory processes [2]. The effect of increased
ambient temperature on mitochondrial morphology and LDs density in living cells of the filamentous
fungus Phycomyces blakesleeanus was investigated. For in vivo imaging of mitochondria and LDs
multiphoton microscopy was used. Multiphoton microscopy enables 3D imaging in high resolution and
reduced photodamage and photobleaching of the sample using IR ultrafast pulsed lasers. Mitochondria
were stained with the vital dye Rhodamine123 (Rh123) which enters these organelles based on their
membrane potential - mitochondria must be healthy/active to stain. A wavelength of 800 nm from Ti:Sa
laser (160 fs pulse duration, 76 MHz repetition rate) was used for two-photon excitation of Rh123. The
laser beam was focused by the Zeiss Plan Neofluar 40x1.3 objective lens and the signal was detected
through a bandpass interference filter MF530/43 (ThorLabs, USA). For LDs staining a Nile Red dye
was used and excited by Yb: KGW laser at 1040 nm [3]. Six morphological types of mitochondria were
observed in the hyphae of this fungus: intermediate type - normal, intermediate with small semicircular
tubules, tubular, elongated tubular, fragmented (small ellipsoid tubule) and fragmented with exclusively
spheroid-shaped mitochondria. Changes in mitochondrial morphology were induced by a small
temperature change. A 3°C increase in ambient temperature, from 22°C, had a dramatic effect on
mitochondrial morphology, inducing the appearance of a predominantly tubular mitochondrial
morphology. The total area percentage of mitochondria showed an increasing trend when grown at
25°C. Increasing the ambient temperature to 25°C induced a statistically significant increase in the
percentage of hyphal area occupied by LDs from 2.9 ± 1.6 to 4.7 ± 2.2 (mean value and SD in percentage
of hyphal area). The observed response to the small temperature increase points to the physiological
adaptation of hyphal metabolism.",
publisher = "Belgrade: Vinča Institute of Nuclear Sciences – National Institute of the Republic of Serbia, University of Belgrade",
journal = "Book of abstracts: 9th International School and Conference on Photonics PHOTONICA 2023 with joint events: Understanding interaction light - biological surfaces possibility for new electronic materials and devices and Biological and bioinspired structures for multispectral surveillance and Quantum sensing integration within microfluidic Lab-on-a Chips for biomedical applications; 2023 Aug 28 - Sep 01; Belgrade, Serbia",
title = "In vivo multiphoton imaging of a filamentous fungus Phycomyces blakesleeanus: the effect of small ambient temperature increase on mitochondrial morphology and lipid droplets density",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6249"
}

Pajić, T., Kozakijević, S., Krmpot, A. J., Živić, M., Todorović, N.,& Rabasović, M. D.. (2023). In vivo multiphoton imaging of a filamentous fungus Phycomyces blakesleeanus: the effect of small ambient temperature increase on mitochondrial morphology and lipid droplets density. in Book of abstracts: 9th International School and Conference on Photonics PHOTONICA 2023 with joint events: Understanding interaction light - biological surfaces possibility for new electronic materials and devices and Biological and bioinspired structures for multispectral surveillance and Quantum sensing integration within microfluidic Lab-on-a Chips for biomedical applications; 2023 Aug 28 - Sep 01; Belgrade, Serbia
Belgrade: Vinča Institute of Nuclear Sciences – National Institute of the Republic of Serbia, University of Belgrade., 84.
https://hdl.handle.net/21.15107/rcub_ibiss_6249

Pajić T, Kozakijević S, Krmpot AJ, Živić M, Todorović N, Rabasović MD. In vivo multiphoton imaging of a filamentous fungus Phycomyces blakesleeanus: the effect of small ambient temperature increase on mitochondrial morphology and lipid droplets density. in Book of abstracts: 9th International School and Conference on Photonics PHOTONICA 2023 with joint events: Understanding interaction light - biological surfaces possibility for new electronic materials and devices and Biological and bioinspired structures for multispectral surveillance and Quantum sensing integration within microfluidic Lab-on-a Chips for biomedical applications; 2023 Aug 28 - Sep 01; Belgrade, Serbia. 2023;:84.
https://hdl.handle.net/21.15107/rcub_ibiss_6249 .

Pajić, Tanja, Kozakijević, Suzana, Krmpot, Aleksandar J., Živić, Miroslav, Todorović, Nataša, Rabasović, Mihailo D., "In vivo multiphoton imaging of a filamentous fungus Phycomyces blakesleeanus: the effect of small ambient temperature increase on mitochondrial morphology and lipid droplets density" in Book of abstracts: 9th International School and Conference on Photonics PHOTONICA 2023 with joint events: Understanding interaction light - biological surfaces possibility for new electronic materials and devices and Biological and bioinspired structures for multispectral surveillance and Quantum sensing integration within microfluidic Lab-on-a Chips for biomedical applications; 2023 Aug 28 - Sep 01; Belgrade, Serbia (2023):84,
https://hdl.handle.net/21.15107/rcub_ibiss_6249 .

TY  - CONF
AU  - Pajić, Tanja
AU  - Stevanović, Katarina
AU  - Todorović, Nataša
AU  - Lević, Steva
AU  - Savić Šević, Svetlana
AU  - Pantelić, Dejan
AU  - Živić, Miroslav
AU  - Rabasović, Mihailo D
AU  - Krmpot, Aleksandar J
PY  - 2023
UR  - https://www.eventclass.org/contxt_emim2023/online-program/session?s=PW36#e609
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6289
AB  - Introduction
Electrophysiology studies of ion channels, in live filamentous fungi by patch clamp method are notpossible due to presence of rigid chitinous cell wall that prevents patch clamp pipette to access theplasma membrane. We present laser nano-surgery of the fungal cell wall that enables patch clampelectrophysiology studies. Similar approaches as one-time reports utilizing nanosecond laser pulseslong time ago were not pursued further [1,2]. Here, we demonstrate reproducible method usingfemtosecond lasers accompanied by two-photon excitation fluorescence (TPEF) imaging of hyphae.
Methods
A wild-type strain of filamentous fungus
Phycomyces blakesleeanus
(Burgeff ) [NRRL 1555(-)] were grownon glass coverslips with hand-etched grid, coated with a thin layer of 50% collagen type I as animmobilizer. Home built nonlinear laser scanning microscope [3,4] utilizing Ti:Sa tunable fs laser wasused for TPEF imaging of hyphae and the cell surgery. The latter is enabled with the custom made add-on in soft ware. Coverslip with hyphae is transferred to another microscope setup for patch clamp,consisting of micromanipulators and precise electronics for pA current measurements. The surgicalincisions and released protoplasts were additionally imaged by scanning electron microscopy for whichtreated hyphae had to undergo critical point drying procedure.
Results/Discussion
Hyphae were stained by Calcofluor White and treated with an exocytosis inhibitor (brefeldin A) and arespiration inhibitor (sodium azide) to prevent cell wall regeneration. Since the cell wall and the plasmamembrane are in the close contact [4] hyphae were kept in hyperosmotic solution to retract thecytoplasm from the cell wall. Surgical spot-wise pattern was precisely positioned at TPEF image ofselected hypha at the place where the plasma membrane was retracted. The dwell time (1s) and thelaser power (4-15mW) were set with fixed repetition rate (76MHz), pulse duration (160fs) and laserwavelength (730nm). Upon the surgery, hyphae were gently deplasmolysed. A protoplast with plasmamembrane accessible for the patch clamp pipette was released through the surgical incision (Fig 1). The>GΩ seal resistance was achieved. Numerous ion channels are recorded in diff erent configurations (oncell, inside-out, whole cell and out-out) (Fig 2).
Conclusions
The whole process (cell surgery + patch clamping) is rather complex and specific steps have to be strictlyfollowed for high success rate and reproducibility. Also, chemicals concentrations, solutions osmolarity,timing and cutting parameters have to be kept in the specified narrow range. Obtained currentrecordings provide valuable information on fungal cell membrane ionic channels.
PB  - European Society for Molecular Imaging
C3  - European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria
T1  - Laser nano-surgery of fungal cell wall to enable patch clamping
SP  - 1095
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6289
ER  - 

@conference{
author = "Pajić, Tanja and Stevanović, Katarina and Todorović, Nataša and Lević, Steva and Savić Šević, Svetlana and Pantelić, Dejan and Živić, Miroslav and Rabasović, Mihailo D and Krmpot, Aleksandar J",
year = "2023",
abstract = "Introduction
Electrophysiology studies of ion channels, in live filamentous fungi by patch clamp method are notpossible due to presence of rigid chitinous cell wall that prevents patch clamp pipette to access theplasma membrane. We present laser nano-surgery of the fungal cell wall that enables patch clampelectrophysiology studies. Similar approaches as one-time reports utilizing nanosecond laser pulseslong time ago were not pursued further [1,2]. Here, we demonstrate reproducible method usingfemtosecond lasers accompanied by two-photon excitation fluorescence (TPEF) imaging of hyphae.
Methods
A wild-type strain of filamentous fungus
Phycomyces blakesleeanus
(Burgeff ) [NRRL 1555(-)] were grownon glass coverslips with hand-etched grid, coated with a thin layer of 50% collagen type I as animmobilizer. Home built nonlinear laser scanning microscope [3,4] utilizing Ti:Sa tunable fs laser wasused for TPEF imaging of hyphae and the cell surgery. The latter is enabled with the custom made add-on in soft ware. Coverslip with hyphae is transferred to another microscope setup for patch clamp,consisting of micromanipulators and precise electronics for pA current measurements. The surgicalincisions and released protoplasts were additionally imaged by scanning electron microscopy for whichtreated hyphae had to undergo critical point drying procedure.
Results/Discussion
Hyphae were stained by Calcofluor White and treated with an exocytosis inhibitor (brefeldin A) and arespiration inhibitor (sodium azide) to prevent cell wall regeneration. Since the cell wall and the plasmamembrane are in the close contact [4] hyphae were kept in hyperosmotic solution to retract thecytoplasm from the cell wall. Surgical spot-wise pattern was precisely positioned at TPEF image ofselected hypha at the place where the plasma membrane was retracted. The dwell time (1s) and thelaser power (4-15mW) were set with fixed repetition rate (76MHz), pulse duration (160fs) and laserwavelength (730nm). Upon the surgery, hyphae were gently deplasmolysed. A protoplast with plasmamembrane accessible for the patch clamp pipette was released through the surgical incision (Fig 1). The>GΩ seal resistance was achieved. Numerous ion channels are recorded in diff erent configurations (oncell, inside-out, whole cell and out-out) (Fig 2).
Conclusions
The whole process (cell surgery + patch clamping) is rather complex and specific steps have to be strictlyfollowed for high success rate and reproducibility. Also, chemicals concentrations, solutions osmolarity,timing and cutting parameters have to be kept in the specified narrow range. Obtained currentrecordings provide valuable information on fungal cell membrane ionic channels.",
publisher = "European Society for Molecular Imaging",
journal = "European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria",
title = "Laser nano-surgery of fungal cell wall to enable patch clamping",
pages = "1095",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6289"
}

Pajić, T., Stevanović, K., Todorović, N., Lević, S., Savić Šević, S., Pantelić, D., Živić, M., Rabasović, M. D.,& Krmpot, A. J.. (2023). Laser nano-surgery of fungal cell wall to enable patch clamping. in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria
European Society for Molecular Imaging., 1095.
https://hdl.handle.net/21.15107/rcub_ibiss_6289

Pajić T, Stevanović K, Todorović N, Lević S, Savić Šević S, Pantelić D, Živić M, Rabasović MD, Krmpot AJ. Laser nano-surgery of fungal cell wall to enable patch clamping. in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria. 2023;:1095.
https://hdl.handle.net/21.15107/rcub_ibiss_6289 .

Pajić, Tanja, Stevanović, Katarina, Todorović, Nataša, Lević, Steva, Savić Šević, Svetlana, Pantelić, Dejan, Živić, Miroslav, Rabasović, Mihailo D, Krmpot, Aleksandar J, "Laser nano-surgery of fungal cell wall to enable patch clamping" in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria (2023):1095,
https://hdl.handle.net/21.15107/rcub_ibiss_6289 .