@article{
author = "Mihailović, Mirjana and Petrović, Miodrag and Grdović, Nevena and Dinić, Svetlana and Uskoković, Aleksandra and Vidaković, Melita and Grigorov, Ilijana and Bogojević, Desanka and Ivanović Matić, Svetlana and Martinović, Vesna and Arambašić Jovanović, Jelena and Joksimović, Danijela and Labus-Blagojević, Svetlana and Poznanović, Goran",
year = "2010, 2010",
abstract = "The enzyme CYP1A is an established biomarker of fish exposure to polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs). The metallothioneins (MT), a family of Cys-rich proteins, bind a wide range of metals and participate in their metabolism. The aim of the study was to examine the correlation between CYP1A and MT expression in commercially important fish species Mullus barbatus and Merluccius merluccius and contaminants (PAHs, PCBs, toxic metals) in seawater and sediment from three localities with different level of contamination in the Adriatic Sea in winter, i.e., Platamuni, Valdanos and the port of Bar. The relative concentration of CYP1A was the highest in both fish species from Bar. Increased concentrations of PCBs in the seawater were observed only in Bar. A species-specific higher increase in the protein concentration of CYP1A was observed in Mullus barbatus compared to Merluccius merluccius. The levels of MT were the highest in Merluccius merluccius from Bar and in Mullus barbatus from Valdanos. The induction of MT correlated with the elevated concentrations of Cu and Pb determined by chemical analysis of the seawater from Bar and Valdanos, respectively. According to the chemical analysis of the seawater and the biological response of the fish, the Platamuni locality exhibited the lowest level of contamination., CYP1A predstavlja dobro okarakterisan biomarker kod riba pri izlaganju policikličnim aromatičnim ugljovodonicima (PAH) i policikličnim bifeholima (PCB). Metalotioneini (MT) pretstavljaju familiju proteina koji vezuju metale i učestvuju u njihovoj metabolizmu, transportu i regulaciji. Cilj ovog rada je bio da se ispitaju korelacije između promena u nivou CYP1A i MT u hepatopankreasu dve komercijalno važne ribe: Mullus barbatus (trlja) i Merluccius merluccius (oslić) i kontaminanatata: PAH, PCB i toksičnih metala) na tri lokaliteta u Jadrnskom moru (Platamuni, Valdanos i luka Bar) u zimu. CYP1A je u najvećem stepenu indukovan na lokalitetu Bar u obe ispitivane vrste, što je u korelaciji sa prisustvom povećane količine PCB u morskoj vodi u Baru. Nivo MT je najveći kod oslića izlovljenog u Baru, a kod trlje u Valdanosu. To je u korelaciji sa izmerenim povećanim koncentracijama bakra u Baru, a olova u Valdanosu. Na osnovu izučavanih parametara, Platamuni su lokalitet sa najmanjim stepenom kontaminacije.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Ekspresija CYPLA i metalotioneina u hepatopankreasu oslića i trlje iz Jadranskog mora, CYP1A and metallothionein expression in the hepatopancreas of merluccius merluccius and mullus barbatus from the Adriatic sea",
number = "8",
volume = "75",
doi = "10.2298/JSC091029083M",
pages = "1149-1159",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_561"
}