TY  - JOUR
AU  - Dabić Zagorac, Dragana Č.
AU  - Fotirić Akšić, Milica M.
AU  - Glavnik, Vesna
AU  - Gašić, Uroš
AU  - Vovk, Irena
AU  - Tešić, Živoslav Lj.
AU  - Natić, Maja M.
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201901271
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3622
AB  - The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.
PB  - Wiley-VCH Verlag
T2  - Journal of Separation Science
T1  - Establishing the chromatographic fingerprints of flavan‐3‐ols and proanthocyanidins from rose hip ( Rosa sp.) species
DO  - 10.1002/jssc.201901271
ER  - 

@article{
author = "Dabić Zagorac, Dragana Č. and Fotirić Akšić, Milica M. and Glavnik, Vesna and Gašić, Uroš and Vovk, Irena and Tešić, Živoslav Lj. and Natić, Maja M.",
year = "2020",
abstract = "The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.",
publisher = "Wiley-VCH Verlag",
journal = "Journal of Separation Science",
title = "Establishing the chromatographic fingerprints of flavan‐3‐ols and proanthocyanidins from rose hip ( Rosa sp.) species",
doi = "10.1002/jssc.201901271"
}

Dabić Zagorac, D. Č., Fotirić Akšić, M. M., Glavnik, V., Gašić, U., Vovk, I., Tešić, Ž. Lj.,& Natić, M. M.. (2020). Establishing the chromatographic fingerprints of flavan‐3‐ols and proanthocyanidins from rose hip ( Rosa sp.) species. in Journal of Separation Science
Wiley-VCH Verlag..
https://doi.org/10.1002/jssc.201901271

Dabić Zagorac DČ, Fotirić Akšić MM, Glavnik V, Gašić U, Vovk I, Tešić ŽL, Natić MM. Establishing the chromatographic fingerprints of flavan‐3‐ols and proanthocyanidins from rose hip ( Rosa sp.) species. in Journal of Separation Science. 2020;.
doi:10.1002/jssc.201901271 .

Dabić Zagorac, Dragana Č., Fotirić Akšić, Milica M., Glavnik, Vesna, Gašić, Uroš, Vovk, Irena, Tešić, Živoslav Lj., Natić, Maja M., "Establishing the chromatographic fingerprints of flavan‐3‐ols and proanthocyanidins from rose hip ( Rosa sp.) species" in Journal of Separation Science (2020),
https://doi.org/10.1002/jssc.201901271 . .

TY  - JOUR
AU  - Dabić Zagorac, Dragana
AU  - Fotirić Akšić, Milica
AU  - Galvnik, Vesna
AU  - Gašić, Uroš
AU  - Vovk, Irena
AU  - Tešić, Živoslav
AU  - Natić, Maja
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4066
AB  - The profile of flavan‐3‐ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan‐3‐ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra‐high‐performance liquid chromatography with linear trap quadrupole‐Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin‐layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.
PB  - Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA
T2  - Journal of Separation Science
T1  - Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species
IS  - 8
VL  - 43
DO  - 10.1002/jssc.201901271
SP  - 1431
EP  - 1439
ER  - 

@article{
author = "Dabić Zagorac, Dragana and Fotirić Akšić, Milica and Galvnik, Vesna and Gašić, Uroš and Vovk, Irena and Tešić, Živoslav and Natić, Maja",
year = "2020",
abstract = "The profile of flavan‐3‐ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan‐3‐ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra‐high‐performance liquid chromatography with linear trap quadrupole‐Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin‐layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.",
publisher = "Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA",
journal = "Journal of Separation Science",
title = "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species",
number = "8",
volume = "43",
doi = "10.1002/jssc.201901271",
pages = "1431-1439"
}

Dabić Zagorac, D., Fotirić Akšić, M., Galvnik, V., Gašić, U., Vovk, I., Tešić, Ž.,& Natić, M.. (2020). Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science
Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA., 43(8), 1431-1439.
https://doi.org/10.1002/jssc.201901271

Dabić Zagorac D, Fotirić Akšić M, Galvnik V, Gašić U, Vovk I, Tešić Ž, Natić M. Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science. 2020;43(8):1431-1439.
doi:10.1002/jssc.201901271 .

Dabić Zagorac, Dragana, Fotirić Akšić, Milica, Galvnik, Vesna, Gašić, Uroš, Vovk, Irena, Tešić, Živoslav, Natić, Maja, "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species" in Journal of Separation Science, 43, no. 8 (2020):1431-1439,
https://doi.org/10.1002/jssc.201901271 . .

TY  - JOUR
AU  - Jurič, Andreja
AU  - Gašić, Uroš
AU  - Brčić Karačonji, Irena
AU  - Jurica, Karlo
AU  - Milojković-Opsenica, Dušanka
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3688
AB  - Despite of the many beneficial health effects of strawberry tree (Arbutus unedo L.) honey due to its strong antioxidant activity derived mostly from polyphenols, a detailed phenolic profile has not been previously studied. The aims of this study were to identify the phenolic compounds, determine the total phenolic content (TPC) and evaluate the radical scavenging activity (RSA) of strawberry tree honey from south Croatia. Fifty-two polyphenolics (twenty-seven phenolic acids and twenty-five flavonoids) were identified using ultra-high-performance liquid chromatograph (UHPLC) coupled to a hybrid mass spectrometer (LTQ Orbitrap MS). Our overall results point to higher TPC (1038 mg gallic acid equivalents per kg of honey) and stronger RSA (3.32 mmol Trolox equivalents per kg of honey) compared to other monofloral honeys. Due to the presence of large quantities of polyphenolic compounds, strawberry tree honey may have great potential as a health promoting food. Line
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - The phenolic profile of strawberry tree (Arbutus unedo L.) honey
IS  - 8
VL  - 85
DO  - 10.2298/JSC191217018J
SP  - 1011
EP  - 1019
ER  - 

@article{
author = "Jurič, Andreja and Gašić, Uroš and Brčić Karačonji, Irena and Jurica, Karlo and Milojković-Opsenica, Dušanka",
year = "2020",
abstract = "Despite of the many beneficial health effects of strawberry tree (Arbutus unedo L.) honey due to its strong antioxidant activity derived mostly from polyphenols, a detailed phenolic profile has not been previously studied. The aims of this study were to identify the phenolic compounds, determine the total phenolic content (TPC) and evaluate the radical scavenging activity (RSA) of strawberry tree honey from south Croatia. Fifty-two polyphenolics (twenty-seven phenolic acids and twenty-five flavonoids) were identified using ultra-high-performance liquid chromatograph (UHPLC) coupled to a hybrid mass spectrometer (LTQ Orbitrap MS). Our overall results point to higher TPC (1038 mg gallic acid equivalents per kg of honey) and stronger RSA (3.32 mmol Trolox equivalents per kg of honey) compared to other monofloral honeys. Due to the presence of large quantities of polyphenolic compounds, strawberry tree honey may have great potential as a health promoting food. Line",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "The phenolic profile of strawberry tree (Arbutus unedo L.) honey",
number = "8",
volume = "85",
doi = "10.2298/JSC191217018J",
pages = "1011-1019"
}

Jurič, A., Gašić, U., Brčić Karačonji, I., Jurica, K.,& Milojković-Opsenica, D.. (2020). The phenolic profile of strawberry tree (Arbutus unedo L.) honey. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 85(8), 1011-1019.
https://doi.org/10.2298/JSC191217018J

Jurič A, Gašić U, Brčić Karačonji I, Jurica K, Milojković-Opsenica D. The phenolic profile of strawberry tree (Arbutus unedo L.) honey. in Journal of the Serbian Chemical Society. 2020;85(8):1011-1019.
doi:10.2298/JSC191217018J .

Jurič, Andreja, Gašić, Uroš, Brčić Karačonji, Irena, Jurica, Karlo, Milojković-Opsenica, Dušanka, "The phenolic profile of strawberry tree (Arbutus unedo L.) honey" in Journal of the Serbian Chemical Society, 85, no. 8 (2020):1011-1019,
https://doi.org/10.2298/JSC191217018J . .

TY  - JOUR
AU  - Stikić, Radmila I.
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Jovanović, Zorica B.
AU  - Gašić, Uroš
AU  - Tešić, Živoslav Lj.
AU  - Djordjević, Nataša Z.
AU  - Savić, Sladjana K.
AU  - Czekus, Borisz G.
AU  - Pešić, Mirjana
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/cche.10278
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3639
AB  - Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health‐promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. 13 phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration, and time‐dependent effects were more expressed in Puno extracts. The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT‐116, as well as the presence of 7 new phenolic and flavonoid compounds.
T2  - Cereal Chemistry
T1  - Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
IS  - 3
VL  - 97
DO  - 10.1002/cche.10278
SP  - cche.10278
SP  - 626
EP  - 633
ER  - 

@article{
author = "Stikić, Radmila I. and Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Jovanović, Zorica B. and Gašić, Uroš and Tešić, Živoslav Lj. and Djordjević, Nataša Z. and Savić, Sladjana K. and Czekus, Borisz G. and Pešić, Mirjana",
year = "2020",
abstract = "Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health‐promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. 13 phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration, and time‐dependent effects were more expressed in Puno extracts. The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT‐116, as well as the presence of 7 new phenolic and flavonoid compounds.",
journal = "Cereal Chemistry",
title = "Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars",
number = "3",
volume = "97",
doi = "10.1002/cche.10278",
pages = "cche.10278-626-633"
}

Stikić, R. I., Milinčić, D. D., Kostić, A. Ž., Jovanović, Z. B., Gašić, U., Tešić, Ž. Lj., Djordjević, N. Z., Savić, S. K., Czekus, B. G.,& Pešić, M.. (2020). Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry, 97(3), cche.10278-633.
https://doi.org/10.1002/cche.10278

Stikić RI, Milinčić DD, Kostić AŽ, Jovanović ZB, Gašić U, Tešić ŽL, Djordjević NZ, Savić SK, Czekus BG, Pešić M. Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry. 2020;97(3):cche.10278-633.
doi:10.1002/cche.10278 .

Stikić, Radmila I., Milinčić, Danijel D., Kostić, Aleksandar Ž., Jovanović, Zorica B., Gašić, Uroš, Tešić, Živoslav Lj., Djordjević, Nataša Z., Savić, Sladjana K., Czekus, Borisz G., Pešić, Mirjana, "Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars" in Cereal Chemistry, 97, no. 3 (2020):cche.10278-633,
https://doi.org/10.1002/cche.10278 . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Trifković, Jelena
AU  - Baošić, Rada
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3600
AB  - The research subject of this paper was a detail physicochemical analysis of 28 honey samples from the northern part of Montenegro. The honey from Montenegro has not been previously studied in such detail. Differentiation between samples, such as honeydew honey and polyfloral honey, was based on electrical conductivity, which was higher than 0.8 mS cm-1 for honeydew honey, as was expected. Other investigated physicochemical parameters (water content, free acids, diastase activity, hydroxymethylfurfural (HMF) content and sugar content) have shown great similarity for all honey samples. The main interest of this study was the identification and quantification of phenolic compounds using ultra-high performance liquid chromatography (UHPLC) with mass spectrometry detection. The results show that honey samples are very rich in phenolic compounds, especially quercetin. Among the 31 quantified phenolic compounds, the most dominant were phenolic acids. The highlight was based on p-hydroxybenzoic acid, p-coumaric acid, caffeic acid and ferulic acid. Considering polyphenolic compounds and sugar content, a high nutritional value can be observed in all samples, with an emphasis on polyfloral honeys, as was confirmed with principal component analysis (PCA). In addition, all honey samples were tested for total phenolic content (TPC) and radical scavenging activity (RSA). The results indicate the higher antioxidant ability of honeys from Montenegro in comparison to some honey samples from other countries in the region.
T2  - RSC Advances
T1  - Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro
IS  - 5
VL  - 10
DO  - 10.1039/c9ra08783d
SP  - 2462
EP  - 2471
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Trifković, Jelena and Baošić, Rada and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2020",
abstract = "The research subject of this paper was a detail physicochemical analysis of 28 honey samples from the northern part of Montenegro. The honey from Montenegro has not been previously studied in such detail. Differentiation between samples, such as honeydew honey and polyfloral honey, was based on electrical conductivity, which was higher than 0.8 mS cm-1 for honeydew honey, as was expected. Other investigated physicochemical parameters (water content, free acids, diastase activity, hydroxymethylfurfural (HMF) content and sugar content) have shown great similarity for all honey samples. The main interest of this study was the identification and quantification of phenolic compounds using ultra-high performance liquid chromatography (UHPLC) with mass spectrometry detection. The results show that honey samples are very rich in phenolic compounds, especially quercetin. Among the 31 quantified phenolic compounds, the most dominant were phenolic acids. The highlight was based on p-hydroxybenzoic acid, p-coumaric acid, caffeic acid and ferulic acid. Considering polyphenolic compounds and sugar content, a high nutritional value can be observed in all samples, with an emphasis on polyfloral honeys, as was confirmed with principal component analysis (PCA). In addition, all honey samples were tested for total phenolic content (TPC) and radical scavenging activity (RSA). The results indicate the higher antioxidant ability of honeys from Montenegro in comparison to some honey samples from other countries in the region.",
journal = "RSC Advances",
title = "Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro",
number = "5",
volume = "10",
doi = "10.1039/c9ra08783d",
pages = "2462-2471"
}

Nešović, M., Gašić, U., Tosti, T., Trifković, J., Baošić, R., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2020). Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro. in RSC Advances, 10(5), 2462-2471.
https://doi.org/10.1039/c9ra08783d

Nešović M, Gašić U, Tosti T, Trifković J, Baošić R, Blagojević S, Ignjatović L, Tešić Ž. Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro. in RSC Advances. 2020;10(5):2462-2471.
doi:10.1039/c9ra08783d .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Trifković, Jelena, Baošić, Rada, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro" in RSC Advances, 10, no. 5 (2020):2462-2471,
https://doi.org/10.1039/c9ra08783d . .

TY  - JOUR
AU  - Šuković, Danijela
AU  - Knežević, Bojana
AU  - Gašić, Uroš
AU  - Sredojević, Milica
AU  - Ćirić, Ivanka
AU  - Todić, Slavica
AU  - Mutić, Jelena
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://www.mdpi.com/2304-8158/9/2/138
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3607
AB  - Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.
T2  - Foods (Basel, Switzerland)
T1  - Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.
IS  - 2
VL  - 9
DO  - 10.3390/foods9020138
SP  - 138
ER  - 

@article{
author = "Šuković, Danijela and Knežević, Bojana and Gašić, Uroš and Sredojević, Milica and Ćirić, Ivanka and Todić, Slavica and Mutić, Jelena and Tešić, Živoslav",
year = "2020",
abstract = "Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.",
journal = "Foods (Basel, Switzerland)",
title = "Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.",
number = "2",
volume = "9",
doi = "10.3390/foods9020138",
pages = "138"
}

Šuković, D., Knežević, B., Gašić, U., Sredojević, M., Ćirić, I., Todić, S., Mutić, J.,& Tešić, Ž.. (2020). Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.. in Foods (Basel, Switzerland), 9(2), 138.
https://doi.org/10.3390/foods9020138

Šuković D, Knežević B, Gašić U, Sredojević M, Ćirić I, Todić S, Mutić J, Tešić Ž. Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.. in Foods (Basel, Switzerland). 2020;9(2):138.
doi:10.3390/foods9020138 .

Šuković, Danijela, Knežević, Bojana, Gašić, Uroš, Sredojević, Milica, Ćirić, Ivanka, Todić, Slavica, Mutić, Jelena, Tešić, Živoslav, "Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro." in Foods (Basel, Switzerland), 9, no. 2 (2020):138,
https://doi.org/10.3390/foods9020138 . .

TY  - JOUR
AU  - Katanić Stanković, Jelena S.
AU  - Srećković, Nikola
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Imbimbo, Paola
AU  - Monti, Daria Maria
AU  - Mihailović, Vladimir
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0926669019309422?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3523
AB  - The plants from genus Salvia, as one of the largest genus in Lamiaceae family, are frequently in use for various purposes, as foods, in cosmetic industry, or in traditional and official medicine. Salvia verticillata L. (liliac sage) is one of sidelined sage species with potential bioactivity, reported in traditional medicine. The aim of this study was to acquire a phytochemical profile of the methanol extract obtained from S. verticillata aerial parts and to evaluate its antioxidant, antimicrobial, and biocompatibility potential. Characteristic compounds of the genus Salvia, such as rosmarinic and caffeic acids, along with their derivatives (e.g. salvianolic and yunnaneic acids isomers) and flavonoids, have been identified by ultrahigh-performance Orbitrap metabolomic fingerprinting as the main phenolic metabolites in S. verticillata. The extract displayed moderate antimicrobial properties and significant antioxidant potential, with the half maximal inhibitory concentration values (IC50) ranging from 33 to 73 μg/mL. Importantly, full biocompatibility of the extract with eukaryotic cell lines was observed up to 72 h. The obtained results revealed the presence of polyphenolic bioactive compounds in S. verticillata extract with promising antioxidant potential and significant biocompatibility. In this regard, S. verticillata can find new perspectives of application as a food ingredient, in cosmetic and pharmaceutical industries, as it represents a valuable source of compounds with prominent health properties, with a special focus on rosmarinic acid.
T2  - Industrial Crops and Products
T1  - Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid
VL  - 143
DO  - 10.1016/J.INDCROP.2019.111932
SP  - 111932
ER  - 

@article{
author = "Katanić Stanković, Jelena S. and Srećković, Nikola and Mišić, Danijela and Gašić, Uroš and Imbimbo, Paola and Monti, Daria Maria and Mihailović, Vladimir",
year = "2020",
abstract = "The plants from genus Salvia, as one of the largest genus in Lamiaceae family, are frequently in use for various purposes, as foods, in cosmetic industry, or in traditional and official medicine. Salvia verticillata L. (liliac sage) is one of sidelined sage species with potential bioactivity, reported in traditional medicine. The aim of this study was to acquire a phytochemical profile of the methanol extract obtained from S. verticillata aerial parts and to evaluate its antioxidant, antimicrobial, and biocompatibility potential. Characteristic compounds of the genus Salvia, such as rosmarinic and caffeic acids, along with their derivatives (e.g. salvianolic and yunnaneic acids isomers) and flavonoids, have been identified by ultrahigh-performance Orbitrap metabolomic fingerprinting as the main phenolic metabolites in S. verticillata. The extract displayed moderate antimicrobial properties and significant antioxidant potential, with the half maximal inhibitory concentration values (IC50) ranging from 33 to 73 μg/mL. Importantly, full biocompatibility of the extract with eukaryotic cell lines was observed up to 72 h. The obtained results revealed the presence of polyphenolic bioactive compounds in S. verticillata extract with promising antioxidant potential and significant biocompatibility. In this regard, S. verticillata can find new perspectives of application as a food ingredient, in cosmetic and pharmaceutical industries, as it represents a valuable source of compounds with prominent health properties, with a special focus on rosmarinic acid.",
journal = "Industrial Crops and Products",
title = "Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid",
volume = "143",
doi = "10.1016/J.INDCROP.2019.111932",
pages = "111932"
}

Katanić Stanković, J. S., Srećković, N., Mišić, D., Gašić, U., Imbimbo, P., Monti, D. M.,& Mihailović, V.. (2020). Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid. in Industrial Crops and Products, 143, 111932.
https://doi.org/10.1016/J.INDCROP.2019.111932

Katanić Stanković JS, Srećković N, Mišić D, Gašić U, Imbimbo P, Monti DM, Mihailović V. Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid. in Industrial Crops and Products. 2020;143:111932.
doi:10.1016/J.INDCROP.2019.111932 .

Katanić Stanković, Jelena S., Srećković, Nikola, Mišić, Danijela, Gašić, Uroš, Imbimbo, Paola, Monti, Daria Maria, Mihailović, Vladimir, "Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid" in Industrial Crops and Products, 143 (2020):111932,
https://doi.org/10.1016/J.INDCROP.2019.111932 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Gašić, Uroš
AU  - Pešić, Mirjana B.
AU  - Stanojević, Sladjana P.
AU  - Barać, Miroljub B.
AU  - Mačukanović-Jocić, Marina P.
AU  - Avramov, Stevan
AU  - Tešić, Živoslav Lj.
PY  - 2019
UR  - http://doi.wiley.com/10.1002/cbdv.201800565
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3286
AB  - This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above‐ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC‐Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C‐glycosides, luteolin O‐glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.
T2  - Chemistry & Biodiversity
T1  - Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species
IS  - 3
VL  - 16
DO  - 10.1002/cbdv.201800565
SP  - e1800565
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Gašić, Uroš and Pešić, Mirjana B. and Stanojević, Sladjana P. and Barać, Miroljub B. and Mačukanović-Jocić, Marina P. and Avramov, Stevan and Tešić, Živoslav Lj.",
year = "2019",
abstract = "This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above‐ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC‐Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C‐glycosides, luteolin O‐glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.",
journal = "Chemistry & Biodiversity",
title = "Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species",
number = "3",
volume = "16",
doi = "10.1002/cbdv.201800565",
pages = "e1800565"
}

Kostić, A. Ž., Gašić, U., Pešić, M. B., Stanojević, S. P., Barać, M. B., Mačukanović-Jocić, M. P., Avramov, S.,& Tešić, Ž. Lj.. (2019). Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species. in Chemistry & Biodiversity, 16(3), e1800565.
https://doi.org/10.1002/cbdv.201800565

Kostić AŽ, Gašić U, Pešić MB, Stanojević SP, Barać MB, Mačukanović-Jocić MP, Avramov S, Tešić ŽL. Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species. in Chemistry & Biodiversity. 2019;16(3):e1800565.
doi:10.1002/cbdv.201800565 .

Kostić, Aleksandar Ž., Gašić, Uroš, Pešić, Mirjana B., Stanojević, Sladjana P., Barać, Miroljub B., Mačukanović-Jocić, Marina P., Avramov, Stevan, Tešić, Živoslav Lj., "Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species" in Chemistry & Biodiversity, 16, no. 3 (2019):e1800565,
https://doi.org/10.1002/cbdv.201800565 . .

TY  - CONF
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5907
AB  - Introduction: Honey is a high energetic sweet food composed, among other components, of
bioactive compounds that attribute therapeutic properties of this product. As antioxidants,
polyphenols are recognized as the major constituents in honey responsible for its health
promoting properties. Bees produce a floral honey from the collected nectar of flowers, and
honeydew honey from collected excrement of insects or plants. Considering that Montenegro
is a country with traditional beekeeping, this is the first time to publish the results of its
honeydew and polyfloral honey samples.
Experimental: Analyzed honey samples, honeydew and polyfloral, were collected from three
locations in norther part of Montenegro, Berane, Bijelo Polje and Pljevlja. Polyphenol profile
of six honey samples was determinated by identifying polyphenols on UHPLC-LTQ OrbiTrap
MS, and by their quantifications on UHPLC-DAD MS/MS.
Results: Of the 32 identified polyphenol compounds, 24 were present in all samples. Appearing
the other eight polyphenol compounds has no constant diversity between the samples. The
similarity between them was confirmed by their quantifications. The highest proportion in total
polyphenols has a quercetin with a mean value of 3.82 mg/kg, followed by p-hydrohybenzoic
acid and p-coumaric acid. Slightly higher content of chrysin, pinocembrin, p-protocatechuic
and vanillic acid can be noticed. The quantifications of the target compounds include 33
polyphenols, and only 16 of them were previously identified. This indicates the occurrence of
certain dissimilarities between two used techniques, which are the consequences of similar
spectra of certain polyphenols and their derivatives.
Conclusion: This is the detailed study of polyphenols in Montenegrin honeys. Based on the
obtained results, it can be seen that the investigated honey samples are very rich in polyphenols,
with no significant differences between honeydew and polyfloral samples. Considering small
areas that cover these three regions of norther part of Montenegro, it can be assumed that small
diversity in plant species causes overlapping in honeybee’s pastures and similarity of samples
PB  - Novi Sad: Faculty of Technology
C3  - Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia
T1  - Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5907
ER  - 

@conference{
author = "Nešović, Milica and Gašić, Uroš and Tešić, Živoslav",
year = "2019",
abstract = "Introduction: Honey is a high energetic sweet food composed, among other components, of
bioactive compounds that attribute therapeutic properties of this product. As antioxidants,
polyphenols are recognized as the major constituents in honey responsible for its health
promoting properties. Bees produce a floral honey from the collected nectar of flowers, and
honeydew honey from collected excrement of insects or plants. Considering that Montenegro
is a country with traditional beekeeping, this is the first time to publish the results of its
honeydew and polyfloral honey samples.
Experimental: Analyzed honey samples, honeydew and polyfloral, were collected from three
locations in norther part of Montenegro, Berane, Bijelo Polje and Pljevlja. Polyphenol profile
of six honey samples was determinated by identifying polyphenols on UHPLC-LTQ OrbiTrap
MS, and by their quantifications on UHPLC-DAD MS/MS.
Results: Of the 32 identified polyphenol compounds, 24 were present in all samples. Appearing
the other eight polyphenol compounds has no constant diversity between the samples. The
similarity between them was confirmed by their quantifications. The highest proportion in total
polyphenols has a quercetin with a mean value of 3.82 mg/kg, followed by p-hydrohybenzoic
acid and p-coumaric acid. Slightly higher content of chrysin, pinocembrin, p-protocatechuic
and vanillic acid can be noticed. The quantifications of the target compounds include 33
polyphenols, and only 16 of them were previously identified. This indicates the occurrence of
certain dissimilarities between two used techniques, which are the consequences of similar
spectra of certain polyphenols and their derivatives.
Conclusion: This is the detailed study of polyphenols in Montenegrin honeys. Based on the
obtained results, it can be seen that the investigated honey samples are very rich in polyphenols,
with no significant differences between honeydew and polyfloral samples. Considering small
areas that cover these three regions of norther part of Montenegro, it can be assumed that small
diversity in plant species causes overlapping in honeybee’s pastures and similarity of samples",
publisher = "Novi Sad: Faculty of Technology",
journal = "Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia",
title = "Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5907"
}

Nešović, M., Gašić, U.,& Tešić, Ž.. (2019). Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro. in Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia
Novi Sad: Faculty of Technology., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_5907

Nešović M, Gašić U, Tešić Ž. Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro. in Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia. 2019;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_5907 .

Nešović, Milica, Gašić, Uroš, Tešić, Živoslav, "Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro" in Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia (2019):39,
https://hdl.handle.net/21.15107/rcub_ibiss_5907 .

TY  - CONF
AU  - Devrnja, Nina
AU  - Ćosić, Tatjana
AU  - Stupar, Sofija
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Šajkunić, Sanja
AU  - Ćirić, Ana
AU  - Savić, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5594
AB  - Methanol and aqueous extracts of French marigold flowers and leaves, with previously determined chemical profiles, were subjected to in vitro antioxidant and antimicrobial assays. The methanol extract of flowers had the highest total phenolic content (TPC) according to the Folin–Ciocalteu method, and UHPLC–LTQ/orbitrap/MS analysis identified the flavonoid aglycones as the dominant class of phenolics in the extract. Likewise, the methanol extract of flowers showed the highest antioxidant activity measured by DPPH and ABTS assays. Generally, the higher TPC correlated with lower IC50 value and stronger antioxidant potential in all extracts. Microdilution test was used for determination of the minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations of French marigold extracts on following microfungi - Aspergillus versicolor, A. niger, Penicillium ochrochloron, P. verrucosum var. cyclopium and Alternaria alternata. The antimicrobial test revealed that P. ochrochloron was the most sensitive while A. niger was the most resistant microfungus to all analyzed extracts. Correspondingly, the methanol extract of flowers was shown to be the most potent one, regarding the antimicrobial activity. Additionally, antimicrobial potential of methanol extract of flowers was tested for in vivo potato tuber protection against A. alternata causing the early blight disease. Based on obtained antioxidant and antimicrobial potential, French marigold methanol extracts could provide an alternative strategy for the controlling expansion of potato pathogens.
PB  - European Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts
SP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5594
ER  - 

@conference{
author = "Devrnja, Nina and Ćosić, Tatjana and Stupar, Sofija and Gašić, Uroš and Tešić, Živoslav and Šajkunić, Sanja and Ćirić, Ana and Savić, Jelena",
year = "2019",
abstract = "Methanol and aqueous extracts of French marigold flowers and leaves, with previously determined chemical profiles, were subjected to in vitro antioxidant and antimicrobial assays. The methanol extract of flowers had the highest total phenolic content (TPC) according to the Folin–Ciocalteu method, and UHPLC–LTQ/orbitrap/MS analysis identified the flavonoid aglycones as the dominant class of phenolics in the extract. Likewise, the methanol extract of flowers showed the highest antioxidant activity measured by DPPH and ABTS assays. Generally, the higher TPC correlated with lower IC50 value and stronger antioxidant potential in all extracts. Microdilution test was used for determination of the minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations of French marigold extracts on following microfungi - Aspergillus versicolor, A. niger, Penicillium ochrochloron, P. verrucosum var. cyclopium and Alternaria alternata. The antimicrobial test revealed that P. ochrochloron was the most sensitive while A. niger was the most resistant microfungus to all analyzed extracts. Correspondingly, the methanol extract of flowers was shown to be the most potent one, regarding the antimicrobial activity. Additionally, antimicrobial potential of methanol extract of flowers was tested for in vivo potato tuber protection against A. alternata causing the early blight disease. Based on obtained antioxidant and antimicrobial potential, French marigold methanol extracts could provide an alternative strategy for the controlling expansion of potato pathogens.",
publisher = "European Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5594"
}

Devrnja, N., Ćosić, T., Stupar, S., Gašić, U., Tešić, Ž., Šajkunić, S., Ćirić, A.,& Savić, J.. (2019). Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
European Federation of Biotechnology., 130.
https://hdl.handle.net/21.15107/rcub_ibiss_5594

Devrnja N, Ćosić T, Stupar S, Gašić U, Tešić Ž, Šajkunić S, Ćirić A, Savić J. Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:130.
https://hdl.handle.net/21.15107/rcub_ibiss_5594 .

Devrnja, Nina, Ćosić, Tatjana, Stupar, Sofija, Gašić, Uroš, Tešić, Živoslav, Šajkunić, Sanja, Ćirić, Ana, Savić, Jelena, "Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):130,
https://hdl.handle.net/21.15107/rcub_ibiss_5594 .

TY  - JOUR
AU  - Vukojević, Vesna
AU  - Đurđić, Slađana
AU  - Stefanović, Violeta
AU  - Trifković, Jelena
AU  - Čakmak, Dragan
AU  - Perović, Veljko
AU  - Mutić, Jelena
PY  - 2019
UR  - http://link.springer.com/10.1007/s11356-018-3982-y
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3242
AB  - The mobility (fractionation) of rare earth elements (REEs) and their possible impacts on ecosystems are still relatively unknown. Soil samples were collected from two sites in central Serbia, an unpolluted mountain region (site 1) and a forest near a city (site 2). In order to investigate REE fractions (acid-soluble/exchangeable, reducible, oxidizable, and residual) in soils, BCR sequential extraction was performed. Additionally, the content of REEs was also determined in stipes and caps of the mushroom Macrolepiota procera, growing in the observed sites. Sc, Y, and lanthanide contents were determined by inductively coupled plasma mass spectrometry (ICP-MS), and results were subjected to multivariate data analysis. Application of pattern recognition technique revealed the existence of two distinguished clusters belonging to different geographical sites and determined by greater levels of Sc, Y, and lanthanides in Goč soil compared to Trstenik soil. Additionally, PCA analysis showed that REEs in soil were concentrated in two groups: the first consisted of elements belonging to light REEs and the second contained heavy REEs. These results suggest that the distribution of REEs in soils could indicate the geographical origin and type of soil. The bioconcentration factors and translocation factors for each REE were also calculated. This study provides baseline data on the rare earth element levels in the wild edible mushroom M. procera, growing in Serbia. In terms of bioconcentration and bioexclusion concept, Sc, Y, and REEs were bioexcluded in M. procera for both studied sites.
PB  - Springer Berlin Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Scandium, yttrium, and lanthanide contents in soil from Serbia and their accumulation in the mushroom Macrolepiota procera (Scop.) Singer.
DO  - 10.1007/s11356-018-3982-y
ER  - 

@article{
author = "Vukojević, Vesna and Đurđić, Slađana and Stefanović, Violeta and Trifković, Jelena and Čakmak, Dragan and Perović, Veljko and Mutić, Jelena",
year = "2019",
abstract = "The mobility (fractionation) of rare earth elements (REEs) and their possible impacts on ecosystems are still relatively unknown. Soil samples were collected from two sites in central Serbia, an unpolluted mountain region (site 1) and a forest near a city (site 2). In order to investigate REE fractions (acid-soluble/exchangeable, reducible, oxidizable, and residual) in soils, BCR sequential extraction was performed. Additionally, the content of REEs was also determined in stipes and caps of the mushroom Macrolepiota procera, growing in the observed sites. Sc, Y, and lanthanide contents were determined by inductively coupled plasma mass spectrometry (ICP-MS), and results were subjected to multivariate data analysis. Application of pattern recognition technique revealed the existence of two distinguished clusters belonging to different geographical sites and determined by greater levels of Sc, Y, and lanthanides in Goč soil compared to Trstenik soil. Additionally, PCA analysis showed that REEs in soil were concentrated in two groups: the first consisted of elements belonging to light REEs and the second contained heavy REEs. These results suggest that the distribution of REEs in soils could indicate the geographical origin and type of soil. The bioconcentration factors and translocation factors for each REE were also calculated. This study provides baseline data on the rare earth element levels in the wild edible mushroom M. procera, growing in Serbia. In terms of bioconcentration and bioexclusion concept, Sc, Y, and REEs were bioexcluded in M. procera for both studied sites.",
publisher = "Springer Berlin Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Scandium, yttrium, and lanthanide contents in soil from Serbia and their accumulation in the mushroom Macrolepiota procera (Scop.) Singer.",
doi = "10.1007/s11356-018-3982-y"
}

Vukojević, V., Đurđić, S., Stefanović, V., Trifković, J., Čakmak, D., Perović, V.,& Mutić, J.. (2019). Scandium, yttrium, and lanthanide contents in soil from Serbia and their accumulation in the mushroom Macrolepiota procera (Scop.) Singer.. in Environmental Science and Pollution Research
Springer Berlin Heidelberg..
https://doi.org/10.1007/s11356-018-3982-y

Vukojević V, Đurđić S, Stefanović V, Trifković J, Čakmak D, Perović V, Mutić J. Scandium, yttrium, and lanthanide contents in soil from Serbia and their accumulation in the mushroom Macrolepiota procera (Scop.) Singer.. in Environmental Science and Pollution Research. 2019;.
doi:10.1007/s11356-018-3982-y .

Vukojević, Vesna, Đurđić, Slađana, Stefanović, Violeta, Trifković, Jelena, Čakmak, Dragan, Perović, Veljko, Mutić, Jelena, "Scandium, yttrium, and lanthanide contents in soil from Serbia and their accumulation in the mushroom Macrolepiota procera (Scop.) Singer." in Environmental Science and Pollution Research (2019),
https://doi.org/10.1007/s11356-018-3982-y . .

TY  - JOUR
AU  - Ćirković, Dušica
AU  - Matijašević, Saša
AU  - Deletić, Nebojša
AU  - Ćirković, Bratislav
AU  - Gašić, Uroš
AU  - Sredojević, Milica
AU  - Jovanović, Zoran
AU  - Djurić, Vladan
AU  - Tešić, Živoslav
PY  - 2019
UR  - https://www.mdpi.com/2073-4395/9/12/822
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3574
AB  - The influence of leaf area and various variants of manual defoliation on the phenolic profile of the Prokupac variety grape berry were investigated in the agroecological conditions of southern Serbia. The following four trial variants of manual defoliation were assessed: Early defoliation—variant I (flowering stage, 50% open flowers); early defoliation—variant II (grape size 3–5 mm); late defoliation—variant III (onset of grape ripening, veraison); and control (no defoliation). The first six leaves of each primary shoot were removed from all defoliated vines. The greatest assimilation area of primary and lateral shoots during the study was observed in the control, i.e., the trial variant with no defoliation. Defoliation significantly decreased the grape yield of the all three defoliated variants in regard to the control. The phenolic profile of the three variants and control was established by analyzing the grape seeds and skin. Based on the collected results for the Prokupac variety, significant differences between the trial variants were established regarding the content of phenols and total polyphenols, as well as radical scavenging activity. Defoliation variants showed a significant effect on the total phenols content of grape skin. In all defoliation variants, as well as in the control, high amounts of ellagic acid were measured. Resveratrol was identified only in grape skin samples of the control variant. The removal of leaves increased the concentration of phenolic compounds in variants where early defoliation was applied. The highest total anthocyanins content was found in 2015 in variant I, where leaves were removed during the full flowering stage.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Agronomy
T1  - The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.)
IS  - 12
VL  - 9
DO  - 10.3390/agronomy9120822
SP  - 822
ER  - 

@article{
author = "Ćirković, Dušica and Matijašević, Saša and Deletić, Nebojša and Ćirković, Bratislav and Gašić, Uroš and Sredojević, Milica and Jovanović, Zoran and Djurić, Vladan and Tešić, Živoslav",
year = "2019",
abstract = "The influence of leaf area and various variants of manual defoliation on the phenolic profile of the Prokupac variety grape berry were investigated in the agroecological conditions of southern Serbia. The following four trial variants of manual defoliation were assessed: Early defoliation—variant I (flowering stage, 50% open flowers); early defoliation—variant II (grape size 3–5 mm); late defoliation—variant III (onset of grape ripening, veraison); and control (no defoliation). The first six leaves of each primary shoot were removed from all defoliated vines. The greatest assimilation area of primary and lateral shoots during the study was observed in the control, i.e., the trial variant with no defoliation. Defoliation significantly decreased the grape yield of the all three defoliated variants in regard to the control. The phenolic profile of the three variants and control was established by analyzing the grape seeds and skin. Based on the collected results for the Prokupac variety, significant differences between the trial variants were established regarding the content of phenols and total polyphenols, as well as radical scavenging activity. Defoliation variants showed a significant effect on the total phenols content of grape skin. In all defoliation variants, as well as in the control, high amounts of ellagic acid were measured. Resveratrol was identified only in grape skin samples of the control variant. The removal of leaves increased the concentration of phenolic compounds in variants where early defoliation was applied. The highest total anthocyanins content was found in 2015 in variant I, where leaves were removed during the full flowering stage.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Agronomy",
title = "The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.)",
number = "12",
volume = "9",
doi = "10.3390/agronomy9120822",
pages = "822"
}

Ćirković, D., Matijašević, S., Deletić, N., Ćirković, B., Gašić, U., Sredojević, M., Jovanović, Z., Djurić, V.,& Tešić, Ž.. (2019). The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.). in Agronomy
Multidisciplinary Digital Publishing Institute., 9(12), 822.
https://doi.org/10.3390/agronomy9120822

Ćirković D, Matijašević S, Deletić N, Ćirković B, Gašić U, Sredojević M, Jovanović Z, Djurić V, Tešić Ž. The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.). in Agronomy. 2019;9(12):822.
doi:10.3390/agronomy9120822 .

Ćirković, Dušica, Matijašević, Saša, Deletić, Nebojša, Ćirković, Bratislav, Gašić, Uroš, Sredojević, Milica, Jovanović, Zoran, Djurić, Vladan, Tešić, Živoslav, "The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.)" in Agronomy, 9, no. 12 (2019):822,
https://doi.org/10.3390/agronomy9120822 . .

TY  - JOUR
AU  - Mosić, Mirjana
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Šikoparija, Branko
AU  - Milojković-Opsenica, Dušanka
PY  - 2019
UR  - https://www.mdpi.com/2218-273X/9/12/783
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3549
AB  - Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Biomolecules
T2  - Biomolecules
T1  - Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.
IS  - 12
VL  - 9
DO  - 10.3390/biom9120783
SP  - 783
ER  - 

@article{
author = "Mosić, Mirjana and Trifković, Jelena and Vovk, Irena and Gašić, Uroš and Tešić, Živoslav and Šikoparija, Branko and Milojković-Opsenica, Dušanka",
year = "2019",
abstract = "Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Biomolecules, Biomolecules",
title = "Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.",
number = "12",
volume = "9",
doi = "10.3390/biom9120783",
pages = "783"
}

Mosić, M., Trifković, J., Vovk, I., Gašić, U., Tešić, Ž., Šikoparija, B.,& Milojković-Opsenica, D.. (2019). Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.. in Biomolecules
Multidisciplinary Digital Publishing Institute., 9(12), 783.
https://doi.org/10.3390/biom9120783

Mosić M, Trifković J, Vovk I, Gašić U, Tešić Ž, Šikoparija B, Milojković-Opsenica D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.. in Biomolecules. 2019;9(12):783.
doi:10.3390/biom9120783 .

Mosić, Mirjana, Trifković, Jelena, Vovk, Irena, Gašić, Uroš, Tešić, Živoslav, Šikoparija, Branko, Milojković-Opsenica, Dušanka, "Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen." in Biomolecules, 9, no. 12 (2019):783,
https://doi.org/10.3390/biom9120783 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Dabić Zagorac, Dragana
AU  - Sredojević, Milica
AU  - Milivojević, Jasminka
AU  - Gašić, Uroš
AU  - Meland, Mekjell
AU  - Natić, Maja
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/23/4310
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3548
AB  - Chemical characterizations of leaves and fruits that were obtained from organically and integrally produced strawberries ('Favette', 'Alba', and 'Clery') and blueberries ('Bluecrop', 'Duke', and 'Nui') from western Serbia were undertaken in this study. Phenolic analysis was done while using ultra-high performance liquid chromatography coupled to a linear ion trap-Orbitrap hybrid mass analyzer, while total phenolic content (TPC), total anthocyanin content (TAC), and radical-scavenging activity (RSA) by spectrophotometry. In general, leaves and fruits from blueberry showed higher levels of TPC and TAC as compared to strawberry. These chemical traits were larger in organic grown fruits and larger in leaves than fruits. The most abundant phenolics in leaves and fruits of blueberry was 5-O-caffeoylquinic acid, followed by quercetin 3-O-galactoside, while catechin, quercetin, and kaempferol 3-O-glucosid were dominant in the leaves and fruits of strawberry. cis, trans-Abscisic acid was detected in all fruit samples, but not in leaves. Blueberries (both fruits and leaves) were separated from strawberries, but only organic blueberry fruits were distinguished from integrated fruits, according to principal component analysis. Quercetin, kaempferol, 5-O-caffeoylquinic acid, ferulic acid, caffeic acid, catechin, p-coumaric acid, and p-hydroxybenzoic acid were the most influential phenolic compounds for the separation. Much higher contents of TPC, RSA, TAC, quercetin 3-O-galactoside, and quercetin were found in fruits and TPC, RSA, catechin, p-hydroxybenzoicacid, p-coumaricacid, and ferulic acid in leaves in all three blueberry cultivars and the strawberry cultivar 'Clery'. These phenolic compounds are good sources of antioxidant compounds with potentially high beneficial effects on human health.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Molecules (Basel, Switzerland)
T1  - Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System.
IS  - 23
VL  - 24
DO  - 10.3390/molecules24234310
SP  - 4310
ER  - 

@article{
author = "Fotirić Akšić, Milica and Dabić Zagorac, Dragana and Sredojević, Milica and Milivojević, Jasminka and Gašić, Uroš and Meland, Mekjell and Natić, Maja",
year = "2019",
abstract = "Chemical characterizations of leaves and fruits that were obtained from organically and integrally produced strawberries ('Favette', 'Alba', and 'Clery') and blueberries ('Bluecrop', 'Duke', and 'Nui') from western Serbia were undertaken in this study. Phenolic analysis was done while using ultra-high performance liquid chromatography coupled to a linear ion trap-Orbitrap hybrid mass analyzer, while total phenolic content (TPC), total anthocyanin content (TAC), and radical-scavenging activity (RSA) by spectrophotometry. In general, leaves and fruits from blueberry showed higher levels of TPC and TAC as compared to strawberry. These chemical traits were larger in organic grown fruits and larger in leaves than fruits. The most abundant phenolics in leaves and fruits of blueberry was 5-O-caffeoylquinic acid, followed by quercetin 3-O-galactoside, while catechin, quercetin, and kaempferol 3-O-glucosid were dominant in the leaves and fruits of strawberry. cis, trans-Abscisic acid was detected in all fruit samples, but not in leaves. Blueberries (both fruits and leaves) were separated from strawberries, but only organic blueberry fruits were distinguished from integrated fruits, according to principal component analysis. Quercetin, kaempferol, 5-O-caffeoylquinic acid, ferulic acid, caffeic acid, catechin, p-coumaric acid, and p-hydroxybenzoic acid were the most influential phenolic compounds for the separation. Much higher contents of TPC, RSA, TAC, quercetin 3-O-galactoside, and quercetin were found in fruits and TPC, RSA, catechin, p-hydroxybenzoicacid, p-coumaricacid, and ferulic acid in leaves in all three blueberry cultivars and the strawberry cultivar 'Clery'. These phenolic compounds are good sources of antioxidant compounds with potentially high beneficial effects on human health.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Molecules (Basel, Switzerland)",
title = "Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System.",
number = "23",
volume = "24",
doi = "10.3390/molecules24234310",
pages = "4310"
}

Fotirić Akšić, M., Dabić Zagorac, D., Sredojević, M., Milivojević, J., Gašić, U., Meland, M.,& Natić, M.. (2019). Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System.. in Molecules (Basel, Switzerland)
Multidisciplinary Digital Publishing Institute., 24(23), 4310.
https://doi.org/10.3390/molecules24234310

Fotirić Akšić M, Dabić Zagorac D, Sredojević M, Milivojević J, Gašić U, Meland M, Natić M. Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System.. in Molecules (Basel, Switzerland). 2019;24(23):4310.
doi:10.3390/molecules24234310 .

Fotirić Akšić, Milica, Dabić Zagorac, Dragana, Sredojević, Milica, Milivojević, Jasminka, Gašić, Uroš, Meland, Mekjell, Natić, Maja, "Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System." in Molecules (Basel, Switzerland), 24, no. 23 (2019):4310,
https://doi.org/10.3390/molecules24234310 . .

TY  - JOUR
AU  - Pejčić, Tomislav
AU  - Tosti, Tomislav
AU  - Džamić, Zoran
AU  - Gašić, Uroš
AU  - Vuksanović, Aleksandar
AU  - Dolićanin, Zana
AU  - Tešić, Živoslav
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/21/3982
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3521
AB  - In recent years, the progress of science and medicine greatly has influenced human life span and health. However, lifestyle habits, like physical activity, smoking cessation, moderate alcohol consumption, diet, and maintaining a normal body weight represent measures that greatly reduce the risk of various diseases. The type of diet is very important for disease development. Numerous epidemiological clinical data confirm that longevity is linked to predominantly plant-based diets and it is related to a long life; whereas the western diet, rich in red meat and fats, increases the risk of oxidative stress and thus the risk of developing various diseases and pre-aging. This review is focused on the bioavailability of polyphenols and the use of polyphenols for the prevention of prostate diseases. Special focus in this paper is placed on the isoflavonoids and flavan-3-ols, subgroups of polyphenols, and their protective effects against the development of prostate diseases.
T2  - Molecules (Basel, Switzerland)
T1  - The Polyphenols as Potential Agents in Prevention and Therapy of Prostate Diseases.
IS  - 21
VL  - 24
DO  - 10.3390/molecules24213982
SP  - 3982
ER  - 

@article{
author = "Pejčić, Tomislav and Tosti, Tomislav and Džamić, Zoran and Gašić, Uroš and Vuksanović, Aleksandar and Dolićanin, Zana and Tešić, Živoslav",
year = "2019",
abstract = "In recent years, the progress of science and medicine greatly has influenced human life span and health. However, lifestyle habits, like physical activity, smoking cessation, moderate alcohol consumption, diet, and maintaining a normal body weight represent measures that greatly reduce the risk of various diseases. The type of diet is very important for disease development. Numerous epidemiological clinical data confirm that longevity is linked to predominantly plant-based diets and it is related to a long life; whereas the western diet, rich in red meat and fats, increases the risk of oxidative stress and thus the risk of developing various diseases and pre-aging. This review is focused on the bioavailability of polyphenols and the use of polyphenols for the prevention of prostate diseases. Special focus in this paper is placed on the isoflavonoids and flavan-3-ols, subgroups of polyphenols, and their protective effects against the development of prostate diseases.",
journal = "Molecules (Basel, Switzerland)",
title = "The Polyphenols as Potential Agents in Prevention and Therapy of Prostate Diseases.",
number = "21",
volume = "24",
doi = "10.3390/molecules24213982",
pages = "3982"
}

Pejčić, T., Tosti, T., Džamić, Z., Gašić, U., Vuksanović, A., Dolićanin, Z.,& Tešić, Ž.. (2019). The Polyphenols as Potential Agents in Prevention and Therapy of Prostate Diseases.. in Molecules (Basel, Switzerland), 24(21), 3982.
https://doi.org/10.3390/molecules24213982

Pejčić T, Tosti T, Džamić Z, Gašić U, Vuksanović A, Dolićanin Z, Tešić Ž. The Polyphenols as Potential Agents in Prevention and Therapy of Prostate Diseases.. in Molecules (Basel, Switzerland). 2019;24(21):3982.
doi:10.3390/molecules24213982 .

Pejčić, Tomislav, Tosti, Tomislav, Džamić, Zoran, Gašić, Uroš, Vuksanović, Aleksandar, Dolićanin, Zana, Tešić, Živoslav, "The Polyphenols as Potential Agents in Prevention and Therapy of Prostate Diseases." in Molecules (Basel, Switzerland), 24, no. 21 (2019):3982,
https://doi.org/10.3390/molecules24213982 . .

TY  - JOUR
AU  - Božunović, Jelena
AU  - Živković, Suzana
AU  - Gašić, Uroš
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Matekalo, Dragana
AU  - Šiler, Branislav
AU  - Soković, Marina
AU  - Tešić, Živoslav
AU  - Mišić, Danijela
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0926669017307999
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2937
AB  - The present study was principally aimed at ascertaining the differences in metabolomics profiles and biological activities between non-hydrolyzed (ME) and hydrolyzed methanol extract (HME) of Centaurium erythraea Rafn. UHPLC–MS/MS Orbitrap analysis showed that the enzymatic hydrolysis of the extract caused changes in β-d-glycoside/aglycone ratio of both flavonoid and secoiridoid compounds. UHPLC/DAD/+HESI−qqqMS characterization and/or quantification of secoiridoid glucosides (SGs) and their aglycones in both ME and HME revealed gentiopicral and erythrocentaurin as the major aglycones, the same metabolic products which appear after the hydrolysis of pure swertiamarin, the dominant secoiridoid glucoside of C. erythraea. SGs played an antioxidant role only in ABTS assay, whilst the remarkable antioxidant potential of C. erythraea methanol extract is ascribed chiefly to phenolics detected in it. Interestingly, antioxidant activities of swertiamarin and sweroside recorded in ABTS assay increased after the compounds have been hydrolyzed, which highlighted their possible antioxidant role during ingestion. Strong antimicrobial activities of ME and HME against a vast array of pathogens, which exceed the effects of the reference antibiotics and antimycotics, largely depended on the amount of secoiridoids in either of the glycosylation forms. Extracts and pure secoiridoids were especially effective against most of the tested Penicillium species. On the other hand, P. funiculosum has evolved an efficient mechanism of detoxification of sub-lethal concentrations of secoiridoid glucosides, involving their biotransformation and complete digestion. The presented findings will contribute to clarify the fate and role of the SGs after C. erythraea ingestion within in vivo systems, and to further promote this remarkable plant as a food preservation additive with significant health benefits.
T2  - Industrial Crops and Products
T2  - Industrial Crops and Products
T1  - In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity
VL  - 111
DO  - 10.1016/j.indcrop.2017.11.040
SP  - 705
EP  - 721
ER  - 

@article{
author = "Božunović, Jelena and Živković, Suzana and Gašić, Uroš and Glamočlija, Jasmina and Ćirić, Ana and Matekalo, Dragana and Šiler, Branislav and Soković, Marina and Tešić, Živoslav and Mišić, Danijela",
year = "2018",
abstract = "The present study was principally aimed at ascertaining the differences in metabolomics profiles and biological activities between non-hydrolyzed (ME) and hydrolyzed methanol extract (HME) of Centaurium erythraea Rafn. UHPLC–MS/MS Orbitrap analysis showed that the enzymatic hydrolysis of the extract caused changes in β-d-glycoside/aglycone ratio of both flavonoid and secoiridoid compounds. UHPLC/DAD/+HESI−qqqMS characterization and/or quantification of secoiridoid glucosides (SGs) and their aglycones in both ME and HME revealed gentiopicral and erythrocentaurin as the major aglycones, the same metabolic products which appear after the hydrolysis of pure swertiamarin, the dominant secoiridoid glucoside of C. erythraea. SGs played an antioxidant role only in ABTS assay, whilst the remarkable antioxidant potential of C. erythraea methanol extract is ascribed chiefly to phenolics detected in it. Interestingly, antioxidant activities of swertiamarin and sweroside recorded in ABTS assay increased after the compounds have been hydrolyzed, which highlighted their possible antioxidant role during ingestion. Strong antimicrobial activities of ME and HME against a vast array of pathogens, which exceed the effects of the reference antibiotics and antimycotics, largely depended on the amount of secoiridoids in either of the glycosylation forms. Extracts and pure secoiridoids were especially effective against most of the tested Penicillium species. On the other hand, P. funiculosum has evolved an efficient mechanism of detoxification of sub-lethal concentrations of secoiridoid glucosides, involving their biotransformation and complete digestion. The presented findings will contribute to clarify the fate and role of the SGs after C. erythraea ingestion within in vivo systems, and to further promote this remarkable plant as a food preservation additive with significant health benefits.",
journal = "Industrial Crops and Products, Industrial Crops and Products",
title = "In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity",
volume = "111",
doi = "10.1016/j.indcrop.2017.11.040",
pages = "705-721"
}

Božunović, J., Živković, S., Gašić, U., Glamočlija, J., Ćirić, A., Matekalo, D., Šiler, B., Soković, M., Tešić, Ž.,& Mišić, D.. (2018). In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity. in Industrial Crops and Products, 111, 705-721.
https://doi.org/10.1016/j.indcrop.2017.11.040

Božunović J, Živković S, Gašić U, Glamočlija J, Ćirić A, Matekalo D, Šiler B, Soković M, Tešić Ž, Mišić D. In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity. in Industrial Crops and Products. 2018;111:705-721.
doi:10.1016/j.indcrop.2017.11.040 .

Božunović, Jelena, Živković, Suzana, Gašić, Uroš, Glamočlija, Jasmina, Ćirić, Ana, Matekalo, Dragana, Šiler, Branislav, Soković, Marina, Tešić, Živoslav, Mišić, Danijela, "In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity" in Industrial Crops and Products, 111 (2018):705-721,
https://doi.org/10.1016/j.indcrop.2017.11.040 . .

TY  - CONF
AU  - Gašić, Uroš
AU  - Stupar, Sofija
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Ćosić, Tatjana
AU  - Ninković, Slavica
AU  - Tešić, Živoslav
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4328
AB  - Aqueous, ethanol and methanol extracts of aerial parts of ornamental French marigold (Tagetes
patula L.), which consist mostly of flavonoids, have been reported to exhibit different biological
activities, including cytotoxic effect on various human cancer cell lines. However, some of the
most potent flavonoids characteristic for the genus Tagetes, such as quercetagetin and patuletin,
are rare in nature. Thus, optimization of extraction process together with employment of sensitive
techniques and detection equipment should be of interest in gaining information about marigold
biologically active compounds. In the present study, separate extracts of leaves and flowers were
obtained from dry plant material by overnight incubation in methanol, dichloromethane or hot
(60 °C) water, followed by ultrasonic bath maceration. UHPLC–LTQ/orbitrap/MS, employed for separation
and identification, revealed the presence of 68 different compounds in total, belonging to
phenolic acids (20 compounds), flavonoid glycosides (37 compounds), and flavonoid aglycones
(11 compounds). The highest number of compounds was detected in methanol extracts, i.e. 55 in
flowers and 60 in leaves, while after the extraction in dichloromethane only 23 compounds were
detected in leaves and 24 in flowers. In both leaves and flowers, the most diversified were flavonoid
glycosides with quercetin and patuletin in 3, 7 or 3,7 di-O- derivativeforms. Aglycon patuletin
was detected in all analyzed extracts, while quercetagetin was detected only in methanol and
dichloromethane extracts of flowers. Obtained data confirmed T. patula extracts as a rich source of
compounds with biological and potential pharmacological values.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4328
ER  - 

@conference{
author = "Gašić, Uroš and Stupar, Sofija and Savić, Jelena and Devrnja, Nina and Ćosić, Tatjana and Ninković, Slavica and Tešić, Živoslav",
year = "2018",
abstract = "Aqueous, ethanol and methanol extracts of aerial parts of ornamental French marigold (Tagetes
patula L.), which consist mostly of flavonoids, have been reported to exhibit different biological
activities, including cytotoxic effect on various human cancer cell lines. However, some of the
most potent flavonoids characteristic for the genus Tagetes, such as quercetagetin and patuletin,
are rare in nature. Thus, optimization of extraction process together with employment of sensitive
techniques and detection equipment should be of interest in gaining information about marigold
biologically active compounds. In the present study, separate extracts of leaves and flowers were
obtained from dry plant material by overnight incubation in methanol, dichloromethane or hot
(60 °C) water, followed by ultrasonic bath maceration. UHPLC–LTQ/orbitrap/MS, employed for separation
and identification, revealed the presence of 68 different compounds in total, belonging to
phenolic acids (20 compounds), flavonoid glycosides (37 compounds), and flavonoid aglycones
(11 compounds). The highest number of compounds was detected in methanol extracts, i.e. 55 in
flowers and 60 in leaves, while after the extraction in dichloromethane only 23 compounds were
detected in leaves and 24 in flowers. In both leaves and flowers, the most diversified were flavonoid
glycosides with quercetin and patuletin in 3, 7 or 3,7 di-O- derivativeforms. Aglycon patuletin
was detected in all analyzed extracts, while quercetagetin was detected only in methanol and
dichloromethane extracts of flowers. Obtained data confirmed T. patula extracts as a rich source of
compounds with biological and potential pharmacological values.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4328"
}

Gašić, U., Stupar, S., Savić, J., Devrnja, N., Ćosić, T., Ninković, S.,& Tešić, Ž.. (2018). UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 124.
https://hdl.handle.net/21.15107/rcub_ibiss_4328

Gašić U, Stupar S, Savić J, Devrnja N, Ćosić T, Ninković S, Tešić Ž. UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;:124.
https://hdl.handle.net/21.15107/rcub_ibiss_4328 .

Gašić, Uroš, Stupar, Sofija, Savić, Jelena, Devrnja, Nina, Ćosić, Tatjana, Ninković, Slavica, Tešić, Živoslav, "UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018):124,
https://hdl.handle.net/21.15107/rcub_ibiss_4328 .

TY  - JOUR
AU  - Jevtić, Bojan
AU  - Nikolovski, Neda
AU  - Stanisavljević, Suzana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Despotović, Jovana
AU  - Samardžić, Jelena
AU  - Miljković, Đorđe
AU  - Timotijević, Gordana
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1756464617304486
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2832
AB  - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4 + T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFκB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.
T2  - Journal of Functional Foods
T1  - Anti-encephalitogenic effects of cucumber leaf extract
VL  - 37
DO  - 10.1016/j.jff.2017.07.060
SP  - 249
EP  - 262
ER  - 

@article{
author = "Jevtić, Bojan and Nikolovski, Neda and Stanisavljević, Suzana and Gašić, Uroš and Mišić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Đorđe and Timotijević, Gordana",
year = "2017",
abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4 + T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFκB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.",
journal = "Journal of Functional Foods",
title = "Anti-encephalitogenic effects of cucumber leaf extract",
volume = "37",
doi = "10.1016/j.jff.2017.07.060",
pages = "249-262"
}

Jevtić, B., Nikolovski, N., Stanisavljević, S., Gašić, U., Mišić, D., Despotović, J., Samardžić, J., Miljković, Đ.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods, 37, 249-262.
https://doi.org/10.1016/j.jff.2017.07.060

Jevtić B, Nikolovski N, Stanisavljević S, Gašić U, Mišić D, Despotović J, Samardžić J, Miljković Đ, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262.
doi:10.1016/j.jff.2017.07.060 .

Jevtić, Bojan, Nikolovski, Neda, Stanisavljević, Suzana, Gašić, Uroš, Mišić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Đorđe, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262,
https://doi.org/10.1016/j.jff.2017.07.060 . .

TY  - JOUR
AU  - Banjanac, Tijana
AU  - Dragićević, Milan
AU  - Šiler, Branislav
AU  - Gašić, Uroš
AU  - Bohanec, Borut
AU  - Nestorović Živković, Jasmina
AU  - Trifunović, Snežana
AU  - Mišić, Danijela
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0031942217301541
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2742
AB  - Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium.
T2  - Phytochemistry
T1  - Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers
VL  - 140
DO  - 10.1016/j.phytochem.2017.04.005
SP  - 27
EP  - 44
ER  - 

@article{
author = "Banjanac, Tijana and Dragićević, Milan and Šiler, Branislav and Gašić, Uroš and Bohanec, Borut and Nestorović Živković, Jasmina and Trifunović, Snežana and Mišić, Danijela",
year = "2017",
abstract = "Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium.",
journal = "Phytochemistry",
title = "Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers",
volume = "140",
doi = "10.1016/j.phytochem.2017.04.005",
pages = "27-44"
}

Banjanac, T., Dragićević, M., Šiler, B., Gašić, U., Bohanec, B., Nestorović Živković, J., Trifunović, S.,& Mišić, D.. (2017). Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers. in Phytochemistry, 140, 27-44.
https://doi.org/10.1016/j.phytochem.2017.04.005

Banjanac T, Dragićević M, Šiler B, Gašić U, Bohanec B, Nestorović Živković J, Trifunović S, Mišić D. Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers. in Phytochemistry. 2017;140:27-44.
doi:10.1016/j.phytochem.2017.04.005 .

Banjanac, Tijana, Dragićević, Milan, Šiler, Branislav, Gašić, Uroš, Bohanec, Borut, Nestorović Živković, Jasmina, Trifunović, Snežana, Mišić, Danijela, "Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers" in Phytochemistry, 140 (2017):27-44,
https://doi.org/10.1016/j.phytochem.2017.04.005 . .

TY  - JOUR
AU  - Paunović, V.
AU  - Kosić, M.
AU  - Đorđević, S.
AU  - Žugić, A.
AU  - Đalinac, N.
AU  - Gašić, U.
AU  - Trajković, Vladimir
AU  - Harhaji-Trajković, Ljubica
PY  - 2016
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27755961
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2563
AB  - Marrubium vulgare is a European medicinal plant with numerous beneficial effects on human health. The aim of the study was to isolate the plant ethanolic extract (MVE) and to investigate its anti-melanoma and anti-glioma effects. MVE was prepared by the modified pharmacopoeial percolation method and characterized by UHPLC-LTQ OrbiTrap MS. MVE dose-dependently reduced viability of melanoma (B16) and glioma (U251) cells, but not peripheral blood mononuclear cells. It arrested cell cycle in S+G2/M phase, which was associated with the activation of MAP kinase p38 and up-regulation of antiproliferative genes p53, p21 and p27. MVE induced oxidative stress, while antioxidants abrogated its antitumor effect. Furthermore, MVE induced mitochondrial depolarization, activation of caspase-9 and -3, Parp cleavage, phosphatidylserine exposure and DNA fragmentation. The mitochondrial apoptotic pathway was associated with the up-regulation of proapoptotic genes Pten, Bak1, Apaf1, and Puma and down-regulation of antiapoptotic genes survivin and Xiap. MVE also stimulated the expression of autophagy-related genes Atg5, Atg7, Atg12, Beclin-1, Gabarab and Sqstm1, as well as LC3-I conversion to the autophagosome associated LC3-II, while autophagy inhibitors exacerbated its cytotoxicity. Finally, the most abundant phenolic components of MVE, ferulic, p-hydroxybenzoic, caffeic and chlorogenic acids, did not exert a profound effect on viability of tumor cells, suggesting that other components individually or in concert are the mediators of the extracts' cytotoxicity. By demonstrating the ability of MVE to inhibit proliferation, induce apoptosis and cytoprotective autophagy, our results suggest that MVE, alone or combined with autophagy inhibitors, could be a good candidate for anti-melanoma and anti-glioma therapy.
T2  - Cellular and molecular biology (Noisy-le-Grand, France)
T1  - Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro.
IS  - 11
VL  - 62
DO  - 10.14715/cmb/2016.62.11.18
SP  - 108
EP  - 114
ER  - 

@article{
author = "Paunović, V. and Kosić, M. and Đorđević, S. and Žugić, A. and Đalinac, N. and Gašić, U. and Trajković, Vladimir and Harhaji-Trajković, Ljubica",
year = "2016",
abstract = "Marrubium vulgare is a European medicinal plant with numerous beneficial effects on human health. The aim of the study was to isolate the plant ethanolic extract (MVE) and to investigate its anti-melanoma and anti-glioma effects. MVE was prepared by the modified pharmacopoeial percolation method and characterized by UHPLC-LTQ OrbiTrap MS. MVE dose-dependently reduced viability of melanoma (B16) and glioma (U251) cells, but not peripheral blood mononuclear cells. It arrested cell cycle in S+G2/M phase, which was associated with the activation of MAP kinase p38 and up-regulation of antiproliferative genes p53, p21 and p27. MVE induced oxidative stress, while antioxidants abrogated its antitumor effect. Furthermore, MVE induced mitochondrial depolarization, activation of caspase-9 and -3, Parp cleavage, phosphatidylserine exposure and DNA fragmentation. The mitochondrial apoptotic pathway was associated with the up-regulation of proapoptotic genes Pten, Bak1, Apaf1, and Puma and down-regulation of antiapoptotic genes survivin and Xiap. MVE also stimulated the expression of autophagy-related genes Atg5, Atg7, Atg12, Beclin-1, Gabarab and Sqstm1, as well as LC3-I conversion to the autophagosome associated LC3-II, while autophagy inhibitors exacerbated its cytotoxicity. Finally, the most abundant phenolic components of MVE, ferulic, p-hydroxybenzoic, caffeic and chlorogenic acids, did not exert a profound effect on viability of tumor cells, suggesting that other components individually or in concert are the mediators of the extracts' cytotoxicity. By demonstrating the ability of MVE to inhibit proliferation, induce apoptosis and cytoprotective autophagy, our results suggest that MVE, alone or combined with autophagy inhibitors, could be a good candidate for anti-melanoma and anti-glioma therapy.",
journal = "Cellular and molecular biology (Noisy-le-Grand, France)",
title = "Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro.",
number = "11",
volume = "62",
doi = "10.14715/cmb/2016.62.11.18",
pages = "108-114"
}

Paunović, V., Kosić, M., Đorđević, S., Žugić, A., Đalinac, N., Gašić, U., Trajković, V.,& Harhaji-Trajković, L.. (2016). Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro.. in Cellular and molecular biology (Noisy-le-Grand, France), 62(11), 108-114.
https://doi.org/10.14715/cmb/2016.62.11.18

Paunović V, Kosić M, Đorđević S, Žugić A, Đalinac N, Gašić U, Trajković V, Harhaji-Trajković L. Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro.. in Cellular and molecular biology (Noisy-le-Grand, France). 2016;62(11):108-114.
doi:10.14715/cmb/2016.62.11.18 .

Paunović, V., Kosić, M., Đorđević, S., Žugić, A., Đalinac, N., Gašić, U., Trajković, Vladimir, Harhaji-Trajković, Ljubica, "Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro." in Cellular and molecular biology (Noisy-le-Grand, France), 62, no. 11 (2016):108-114,
https://doi.org/10.14715/cmb/2016.62.11.18 . .