TY  - CHAP
AU  - Davidović, Slobodan
AU  - Aleksić, Jelena
AU  - Stevanović, Milena
AU  - Kovačević-Grujičić, Nataša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6387
AB  - Mitohondrijska DNK (mtDNK) se odlikuje nizom osobina koje je čine pogodnom za istraživanja evolutivne
istorije ljudskih populacija koja se zasniva na molekularnim markerima ženske linije nasleđivanja. Tokom
poslednje decenije publikovano je više naučnih radova u kojima je analizirana varijabilnost mtDNK u populaciji Srbije primenom markera različite rezolucije uključujući i kompletne genome. U skladu sa očekivanjima zasnovanim na istorijskim, arheološkim i drugim izvorima koji govore u prilog veoma kompleksne istorije populacija na Balkanskom poluostrvu, mtDNK podaci su potvrdili da se srpska populacija odlikuje visokim nivoom raznovrsnosti mtDNK koji je posledica izuzetno složene dinamike ove populacije tokom vremena. Današnji mtDNK profil populacije Srbije ne odstupa od matrilinealnog profila karakterističnog za druge evropske populacije, a genetičke distance pokazuju da ova populacija zauzima centralnu poziciju unutar grupe južnoslovenskih populacija koje se odlikuju visokom heterogenošću. Srpska populacija deli najveći procenat mtDNK haplotipova sa geografski bliskim populacijama Balkanskog poluostrva koje pripadaju južnoslovenskoj grupi, gde su uočeni i potencijalno privatni haplotipovi. Na osnovu filogenetske i filogeografske analize kompletnih mitogenoma u srpskoj populaciji detektovane su retke mtDNK linije,karakteristične za druge regione, poput Bliskog istoka (N1b, HV2), istočne Azije (D4) i Afrike (L2a1), kao i one koje su potencijalno specifične za Balkansko poluostrvo, poput K1a13a1, U4c1b1 i H6a2b. Pored toga, srpska populacija deli određeni broj mtDNK podhaplogrupa sa istočno- i zapadnoslovenskim populacijama kao i sa germanskim populacijama severne i srednje Evrope. Istraživanja varijabilnosti mtDNK su pokazala da se izuzetno velika raznovrsnost mtDNK savremene populacije Srbije može objasniti genetičkim doprinosom kako slovenskih i germanskih, tako i pre-slovenskih populacija koje su naseljavale Balkansko poluostrvo pre Velike seobe naroda.
AB  - The mitochondrial DNA (mtDNA) is characterized by a number of features that make it suitable for study-
ing the evolutionary history of human populations based on molecular markers with the female-specific
line of inheritance. During the last decade, several scientific papers were published in which the mtDNA
variability in the population of Serbia was analyzed using markers of different resolution including com-
plete mitogenomes. In accordance with expectations based on historical, archaeological and other sources
that speak in favor of a very complex history of populations on the Balkan Peninsula, mtDNA data con-
firmed that Serbian population is characterized by a high level of mtDNA diversity, which is a consequence
of the exceptionally complex dynamics of this population over time. Today’s mtDNA profile of the Serbian
population does not differ from the matrilineal landscape characteristic of other European populations,
and according to genetic distances, this population occupies a central position within the group of South-
Slavic populations characterized by high heterogeneity. The Serbian population shares the highest per-
centage of mtDNA haplotypes with the geographically close populations of the Balkan Peninsula
belonging to the South-Slavic group, where potentially private haplotypes were also observed. Phyloge-
netic and phylogeographic analysis of complete mitogenomes in the Serbian population revealed rare
mtDNA lines, characteristic of other regions, such as the Middle East (N1b, HV2), East Asia (D4) and Africa
(L2a1), as well as those that are potentially specific for Balkan Peninsula, like K1a13a1, U4c1b1 and H6a2b.
In addition, Serbian population shares a certain number of mtDNA subhaplogroups with East- and West-
Slavic populations as well as with the Germanic populations of Northern and Central Europe. Studies of
mtDNA variability have shown that the exceptionally high mtDNA diversity in contemporary Serbian pop-
ulation may be associated with the genetic contribution of both Slavic and Germanic, as well as pre-Slavic
populations that inhabited the Balkan Peninsula before the Great Migration.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Trends in Molecular Biology
T1  - Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije
T1  - Mitochondrial gene pool variability of the residents of the Republic of Serbia
IS  - 3
SP  - 18
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6387
ER  - 

@inbook{
author = "Davidović, Slobodan and Aleksić, Jelena and Stevanović, Milena and Kovačević-Grujičić, Nataša",
year = "2023",
abstract = "Mitohondrijska DNK (mtDNK) se odlikuje nizom osobina koje je čine pogodnom za istraživanja evolutivne
istorije ljudskih populacija koja se zasniva na molekularnim markerima ženske linije nasleđivanja. Tokom
poslednje decenije publikovano je više naučnih radova u kojima je analizirana varijabilnost mtDNK u populaciji Srbije primenom markera različite rezolucije uključujući i kompletne genome. U skladu sa očekivanjima zasnovanim na istorijskim, arheološkim i drugim izvorima koji govore u prilog veoma kompleksne istorije populacija na Balkanskom poluostrvu, mtDNK podaci su potvrdili da se srpska populacija odlikuje visokim nivoom raznovrsnosti mtDNK koji je posledica izuzetno složene dinamike ove populacije tokom vremena. Današnji mtDNK profil populacije Srbije ne odstupa od matrilinealnog profila karakterističnog za druge evropske populacije, a genetičke distance pokazuju da ova populacija zauzima centralnu poziciju unutar grupe južnoslovenskih populacija koje se odlikuju visokom heterogenošću. Srpska populacija deli najveći procenat mtDNK haplotipova sa geografski bliskim populacijama Balkanskog poluostrva koje pripadaju južnoslovenskoj grupi, gde su uočeni i potencijalno privatni haplotipovi. Na osnovu filogenetske i filogeografske analize kompletnih mitogenoma u srpskoj populaciji detektovane su retke mtDNK linije,karakteristične za druge regione, poput Bliskog istoka (N1b, HV2), istočne Azije (D4) i Afrike (L2a1), kao i one koje su potencijalno specifične za Balkansko poluostrvo, poput K1a13a1, U4c1b1 i H6a2b. Pored toga, srpska populacija deli određeni broj mtDNK podhaplogrupa sa istočno- i zapadnoslovenskim populacijama kao i sa germanskim populacijama severne i srednje Evrope. Istraživanja varijabilnosti mtDNK su pokazala da se izuzetno velika raznovrsnost mtDNK savremene populacije Srbije može objasniti genetičkim doprinosom kako slovenskih i germanskih, tako i pre-slovenskih populacija koje su naseljavale Balkansko poluostrvo pre Velike seobe naroda., The mitochondrial DNA (mtDNA) is characterized by a number of features that make it suitable for study-
ing the evolutionary history of human populations based on molecular markers with the female-specific
line of inheritance. During the last decade, several scientific papers were published in which the mtDNA
variability in the population of Serbia was analyzed using markers of different resolution including com-
plete mitogenomes. In accordance with expectations based on historical, archaeological and other sources
that speak in favor of a very complex history of populations on the Balkan Peninsula, mtDNA data con-
firmed that Serbian population is characterized by a high level of mtDNA diversity, which is a consequence
of the exceptionally complex dynamics of this population over time. Today’s mtDNA profile of the Serbian
population does not differ from the matrilineal landscape characteristic of other European populations,
and according to genetic distances, this population occupies a central position within the group of South-
Slavic populations characterized by high heterogeneity. The Serbian population shares the highest per-
centage of mtDNA haplotypes with the geographically close populations of the Balkan Peninsula
belonging to the South-Slavic group, where potentially private haplotypes were also observed. Phyloge-
netic and phylogeographic analysis of complete mitogenomes in the Serbian population revealed rare
mtDNA lines, characteristic of other regions, such as the Middle East (N1b, HV2), East Asia (D4) and Africa
(L2a1), as well as those that are potentially specific for Balkan Peninsula, like K1a13a1, U4c1b1 and H6a2b.
In addition, Serbian population shares a certain number of mtDNA subhaplogroups with East- and West-
Slavic populations as well as with the Germanic populations of Northern and Central Europe. Studies of
mtDNA variability have shown that the exceptionally high mtDNA diversity in contemporary Serbian pop-
ulation may be associated with the genetic contribution of both Slavic and Germanic, as well as pre-Slavic
populations that inhabited the Balkan Peninsula before the Great Migration.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Trends in Molecular Biology",
booktitle = "Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije, Mitochondrial gene pool variability of the residents of the Republic of Serbia",
number = "3",
pages = "18-36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6387"
}

Davidović, S., Aleksić, J., Stevanović, M.,& Kovačević-Grujičić, N.. (2023). Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije. in Trends in Molecular Biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(3), 18-36.
https://hdl.handle.net/21.15107/rcub_ibiss_6387

Davidović S, Aleksić J, Stevanović M, Kovačević-Grujičić N. Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije. in Trends in Molecular Biology. 2023;(3):18-36.
https://hdl.handle.net/21.15107/rcub_ibiss_6387 .

Davidović, Slobodan, Aleksić, Jelena, Stevanović, Milena, Kovačević-Grujičić, Nataša, "Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije" in Trends in Molecular Biology, no. 3 (2023):18-36,
https://hdl.handle.net/21.15107/rcub_ibiss_6387 .

TY  - JOUR
AU  - Kloska, Anna
AU  - Giełczyk, Agata
AU  - Grzybowski, Tomasz
AU  - Płoski, Rafał
AU  - Kloska, Sylwester
AU  - Marciniak, Tomasz
AU  - Pałczynski, Krzysztof
AU  - Rogalla-Ładniak, Urszula
AU  - Malyarchuk, Boris
AU  - Derenko, Miroslava
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
AU  - Davidović, Slobodan
AU  - Spólnicka, Magdalena
AU  - Zubanska, Magdalena
AU  - Wozniak, Marcin
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6321
AB  - Abstract

Data obtained with the use of massive parallel sequencing (MPS) can be valuable in population genetics studies. In particular, such data harbor the potential for distinguishing samples from different populations, especially from those coming from adjacent populations of common origin. Machine learning (ML) techniques seem to be especially well suited for analyzing large datasets obtained using MPS. The Slavic populations constitute about a third of the population of Europe and inhabit a large area of the continent, while being relatively closely related in population genetics terms. In this proof-of-concept study, various ML techniques were used to classify DNA samples from Slavic and non-Slavic individuals. The primary objective of this study was to empirically evaluate the feasibility of discerning the genetic provenance of individuals of Slavic descent who exhibit genetic similarity, with the overarching goal of categorizing DNA specimens derived from diverse Slavic population representatives. Raw sequencing data were pre-processed, to obtain a 1200 character-long binary vector. A total of three classifiers were used—Random Forest, Support Vector Machine (SVM), and XGBoost. The most-promising results were obtained using SVM with a linear kernel, with 99.9% accuracy and F1-scores of 0.9846–1.000 for all classes.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe
IS  - 20
VL  - 24
DO  - 10.3390/ijms242015095
SP  - 15095
ER  - 

@article{
author = "Kloska, Anna and Giełczyk, Agata and Grzybowski, Tomasz and Płoski, Rafał and Kloska, Sylwester and Marciniak, Tomasz and Pałczynski, Krzysztof and Rogalla-Ładniak, Urszula and Malyarchuk, Boris and Derenko, Miroslava and Kovačević-Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela and Davidović, Slobodan and Spólnicka, Magdalena and Zubanska, Magdalena and Wozniak, Marcin",
year = "2023",
abstract = "Abstract

Data obtained with the use of massive parallel sequencing (MPS) can be valuable in population genetics studies. In particular, such data harbor the potential for distinguishing samples from different populations, especially from those coming from adjacent populations of common origin. Machine learning (ML) techniques seem to be especially well suited for analyzing large datasets obtained using MPS. The Slavic populations constitute about a third of the population of Europe and inhabit a large area of the continent, while being relatively closely related in population genetics terms. In this proof-of-concept study, various ML techniques were used to classify DNA samples from Slavic and non-Slavic individuals. The primary objective of this study was to empirically evaluate the feasibility of discerning the genetic provenance of individuals of Slavic descent who exhibit genetic similarity, with the overarching goal of categorizing DNA specimens derived from diverse Slavic population representatives. Raw sequencing data were pre-processed, to obtain a 1200 character-long binary vector. A total of three classifiers were used—Random Forest, Support Vector Machine (SVM), and XGBoost. The most-promising results were obtained using SVM with a linear kernel, with 99.9% accuracy and F1-scores of 0.9846–1.000 for all classes.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe",
number = "20",
volume = "24",
doi = "10.3390/ijms242015095",
pages = "15095"
}

Kloska, A., Giełczyk, A., Grzybowski, T., Płoski, R., Kloska, S., Marciniak, T., Pałczynski, K., Rogalla-Ładniak, U., Malyarchuk, B., Derenko, M., Kovačević-Grujičić, N., Stevanović, M., Drakulić, D., Davidović, S., Spólnicka, M., Zubanska, M.,& Wozniak, M.. (2023). A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe. in International Journal of Molecular Sciences
Basel: MDPI., 24(20), 15095.
https://doi.org/10.3390/ijms242015095

Kloska A, Giełczyk A, Grzybowski T, Płoski R, Kloska S, Marciniak T, Pałczynski K, Rogalla-Ładniak U, Malyarchuk B, Derenko M, Kovačević-Grujičić N, Stevanović M, Drakulić D, Davidović S, Spólnicka M, Zubanska M, Wozniak M. A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe. in International Journal of Molecular Sciences. 2023;24(20):15095.
doi:10.3390/ijms242015095 .

Kloska, Anna, Giełczyk, Agata, Grzybowski, Tomasz, Płoski, Rafał, Kloska, Sylwester, Marciniak, Tomasz, Pałczynski, Krzysztof, Rogalla-Ładniak, Urszula, Malyarchuk, Boris, Derenko, Miroslava, Kovačević-Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, Davidović, Slobodan, Spólnicka, Magdalena, Zubanska, Magdalena, Wozniak, Marcin, "A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe" in International Journal of Molecular Sciences, 24, no. 20 (2023):15095,
https://doi.org/10.3390/ijms242015095 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Dias, Maria Inês
AU  - Zengin, Gokhan
AU  - Barros, Lillian
AU  - Ivanov, Marija
AU  - Gašić, Uroš
AU  - Rajčević, Nemanja
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C.F.R.
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4871
AB  - Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa extract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic effect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.
PB  - Dortmund: EXCLI Journal
T2  - EXCLI Journal
T1  - Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line
VL  - 21
DO  - 10.17179/excli2021-4487
SP  - 387
EP  - 399
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Dias, Maria Inês and Zengin, Gokhan and Barros, Lillian and Ivanov, Marija and Gašić, Uroš and Rajčević, Nemanja and Stevanović, Milena and Ferreira, Isabel C.F.R. and Soković, Marina",
year = "2022",
abstract = "Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa extract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic effect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.",
publisher = "Dortmund: EXCLI Journal",
journal = "EXCLI Journal",
title = "Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line",
volume = "21",
doi = "10.17179/excli2021-4487",
pages = "387-399"
}

Stojković, D., Drakulić, D., Dias, M. I., Zengin, G., Barros, L., Ivanov, M., Gašić, U., Rajčević, N., Stevanović, M., Ferreira, I. C.F.R.,& Soković, M.. (2022). Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line. in EXCLI Journal
Dortmund: EXCLI Journal., 21, 387-399.
https://doi.org/10.17179/excli2021-4487

Stojković D, Drakulić D, Dias MI, Zengin G, Barros L, Ivanov M, Gašić U, Rajčević N, Stevanović M, Ferreira IC, Soković M. Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line. in EXCLI Journal. 2022;21:387-399.
doi:10.17179/excli2021-4487 .

Stojković, Dejan, Drakulić, Danijela, Dias, Maria Inês, Zengin, Gokhan, Barros, Lillian, Ivanov, Marija, Gašić, Uroš, Rajčević, Nemanja, Stevanović, Milena, Ferreira, Isabel C.F.R., Soković, Marina, "Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line" in EXCLI Journal, 21 (2022):387-399,
https://doi.org/10.17179/excli2021-4487 . .

TY  - JOUR
AU  - Holub, Karolina
AU  - Malyarchuk, Boris
AU  - Derenko, Miroslava
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
AU  - Davidović, Slobodan
AU  - Grzybowski, Tomasz
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5555
AB  - Genetic markers for the prediction of biogeographical ancestry have proved to be effective tools for law enforcement agencies for many years now. In this study, we attempted to assess the potential of insertion-deletion markers (InDel) and microsatellites (STRs) as subsidiary polymorphisms for inference of Slavic population ancestry. For that purpose, we genotyped Slavic-speaking populations samples from Belarus, the Czech Republic, Poland, Serbia, Ukraine and Russia in 46 InDels and 15 STRs by PCR and capillary electrophoresis and analyzed for between-population differentiation with the use of distance-based methods (FST, principal component analysis and multidimensional scaling).
Additionally, we studied a sample from a Polish individual of well-documented genealogy whose biogeographic ancestry had previously been inferred by commercial genomic services using autosomal single nucleotide polymorphisms (SNPs), mitochondrial DNA and Y-SNP markers. For comparative purposes, we used genotype data collected in the “forInDel” browser and allele frequencies from previously published papers. The results obtained for InDels and STRs show that the Slavic populations constitute a genetically homogeneous group, with the exception of the Czechs differing clearly from the other tested populations. The analysis of the known Polish sample in the Snipper application proves the usefulness of the InDel markers on the continental level only. Conversely, microsatellites not only improve prediction, but are also informative if considered as an independent set of ancestry markers.
PB  - Polish Society of Forensic Medicine and Criminology
T2  - Archives of Forensic Medicine and Criminology
T1  - Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry
IS  - 3
VL  - 72
DO  - 10.4467/16891716AMSIK.22.015.17393
SP  - 120
EP  - 137
ER  - 

@article{
author = "Holub, Karolina and Malyarchuk, Boris and Derenko, Miroslava and Kovačević-Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela and Davidović, Slobodan and Grzybowski, Tomasz",
year = "2022",
abstract = "Genetic markers for the prediction of biogeographical ancestry have proved to be effective tools for law enforcement agencies for many years now. In this study, we attempted to assess the potential of insertion-deletion markers (InDel) and microsatellites (STRs) as subsidiary polymorphisms for inference of Slavic population ancestry. For that purpose, we genotyped Slavic-speaking populations samples from Belarus, the Czech Republic, Poland, Serbia, Ukraine and Russia in 46 InDels and 15 STRs by PCR and capillary electrophoresis and analyzed for between-population differentiation with the use of distance-based methods (FST, principal component analysis and multidimensional scaling).
Additionally, we studied a sample from a Polish individual of well-documented genealogy whose biogeographic ancestry had previously been inferred by commercial genomic services using autosomal single nucleotide polymorphisms (SNPs), mitochondrial DNA and Y-SNP markers. For comparative purposes, we used genotype data collected in the “forInDel” browser and allele frequencies from previously published papers. The results obtained for InDels and STRs show that the Slavic populations constitute a genetically homogeneous group, with the exception of the Czechs differing clearly from the other tested populations. The analysis of the known Polish sample in the Snipper application proves the usefulness of the InDel markers on the continental level only. Conversely, microsatellites not only improve prediction, but are also informative if considered as an independent set of ancestry markers.",
publisher = "Polish Society of Forensic Medicine and Criminology",
journal = "Archives of Forensic Medicine and Criminology",
title = "Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry",
number = "3",
volume = "72",
doi = "10.4467/16891716AMSIK.22.015.17393",
pages = "120-137"
}

Holub, K., Malyarchuk, B., Derenko, M., Kovačević-Grujičić, N., Stevanović, M., Drakulić, D., Davidović, S.,& Grzybowski, T.. (2022). Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry. in Archives of Forensic Medicine and Criminology
Polish Society of Forensic Medicine and Criminology., 72(3), 120-137.
https://doi.org/10.4467/16891716AMSIK.22.015.17393

Holub K, Malyarchuk B, Derenko M, Kovačević-Grujičić N, Stevanović M, Drakulić D, Davidović S, Grzybowski T. Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry. in Archives of Forensic Medicine and Criminology. 2022;72(3):120-137.
doi:10.4467/16891716AMSIK.22.015.17393 .

Holub, Karolina, Malyarchuk, Boris, Derenko, Miroslava, Kovačević-Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, Davidović, Slobodan, Grzybowski, Tomasz, "Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry" in Archives of Forensic Medicine and Criminology, 72, no. 3 (2022):120-137,
https://doi.org/10.4467/16891716AMSIK.22.015.17393 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Drakulić, Danijela
AU  - Stevanović, Milena
AU  - Rajčević, Nemanja
AU  - Stojković, Dejan
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669021010256
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4696
AB  - Salvia nemorosa L. is a rich source of bioactive constituents. We have used different solvents to obtain 14 different extracts in order to evaluate solvents impact on S. nemorosa inflorescences bioactivities. Firstly, the extraction yield, chemical composition and cytotoxic properties towards HaCaT cell line were elaborated. Afterwards, we have studied the extracts bioactivity in terms of wound healing, antioxidant and antidiabetic potentials as well as antimicrobial and antibiofilm capacities; followed by the determination of the antimicrobial mechanism. The extracts yield ranged from 3.03% to 15.75% and they were dominated by epigallocatechin gallate and rosmarinic acid. The majority of extracts did not possess cytotoxic properties towards human keratinocytes. Butanol was the most appropriate solvent for the improvement of wound healing capacity. Combined extraction with ethanol and water resulted in the highest antioxidant potential of the extracts that could be attributed to the high abundance of detected polyphenols. Extraction with butanol led to the increased inhibition towards enzymes linked to type 2-diabetes and increase in antimicrobial capacities. The extracts have shown antibiofilm potential towards Staphylococcus lugdunensis biofilms with more than 90% inhibition for the most active ones. The selected extract EB7 reduced bacterial ability to adhere to human keratinocytes and provided immunomodulatory action in HaCaT cells exposed to S. lugdunensis. The antibacterial mechanism of action of the same extract was associated with moderate perturbation in the cell membrane integrity and with the reduction in bacterial mitochondrial activity. S. nemorosa inflorescences were abundant sources of bioactive polyphenols and with different extraction solvents their bioactivities were further improved. This study reported for the first time the effects of extraction solvents on various bioactivities of S. nemorosa inflorescences, supporting S. nemorosa role as a natural bioactive therapeutic agent with possible antioxidant, antidiabetic and antimicrobial applications.
PB  - Amsterdam: Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents
VL  - 175
DO  - 10.1016/j.indcrop.2021.114260
SP  - 114260
ER  - 

@article{
author = "Ivanov, Marija and Božunović, Jelena and Gašić, Uroš and Drakulić, Danijela and Stevanović, Milena and Rajčević, Nemanja and Stojković, Dejan",
year = "2022",
abstract = "Salvia nemorosa L. is a rich source of bioactive constituents. We have used different solvents to obtain 14 different extracts in order to evaluate solvents impact on S. nemorosa inflorescences bioactivities. Firstly, the extraction yield, chemical composition and cytotoxic properties towards HaCaT cell line were elaborated. Afterwards, we have studied the extracts bioactivity in terms of wound healing, antioxidant and antidiabetic potentials as well as antimicrobial and antibiofilm capacities; followed by the determination of the antimicrobial mechanism. The extracts yield ranged from 3.03% to 15.75% and they were dominated by epigallocatechin gallate and rosmarinic acid. The majority of extracts did not possess cytotoxic properties towards human keratinocytes. Butanol was the most appropriate solvent for the improvement of wound healing capacity. Combined extraction with ethanol and water resulted in the highest antioxidant potential of the extracts that could be attributed to the high abundance of detected polyphenols. Extraction with butanol led to the increased inhibition towards enzymes linked to type 2-diabetes and increase in antimicrobial capacities. The extracts have shown antibiofilm potential towards Staphylococcus lugdunensis biofilms with more than 90% inhibition for the most active ones. The selected extract EB7 reduced bacterial ability to adhere to human keratinocytes and provided immunomodulatory action in HaCaT cells exposed to S. lugdunensis. The antibacterial mechanism of action of the same extract was associated with moderate perturbation in the cell membrane integrity and with the reduction in bacterial mitochondrial activity. S. nemorosa inflorescences were abundant sources of bioactive polyphenols and with different extraction solvents their bioactivities were further improved. This study reported for the first time the effects of extraction solvents on various bioactivities of S. nemorosa inflorescences, supporting S. nemorosa role as a natural bioactive therapeutic agent with possible antioxidant, antidiabetic and antimicrobial applications.",
publisher = "Amsterdam: Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents",
volume = "175",
doi = "10.1016/j.indcrop.2021.114260",
pages = "114260"
}

Ivanov, M., Božunović, J., Gašić, U., Drakulić, D., Stevanović, M., Rajčević, N.,& Stojković, D.. (2022). Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents. in Industrial Crops and Products
Amsterdam: Elsevier B.V.., 175, 114260.
https://doi.org/10.1016/j.indcrop.2021.114260

Ivanov M, Božunović J, Gašić U, Drakulić D, Stevanović M, Rajčević N, Stojković D. Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents. in Industrial Crops and Products. 2022;175:114260.
doi:10.1016/j.indcrop.2021.114260 .

Ivanov, Marija, Božunović, Jelena, Gašić, Uroš, Drakulić, Danijela, Stevanović, Milena, Rajčević, Nemanja, Stojković, Dejan, "Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents" in Industrial Crops and Products, 175 (2022):114260,
https://doi.org/10.1016/j.indcrop.2021.114260 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Gašić, Uroš
AU  - Drakulić, Danijela
AU  - Zengin, Gokhan
AU  - Stevanović, Milena
AU  - Rajčević, Nemanja
AU  - Soković, Marina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4229
AB  - Structural diversity of biologically active compounds identified in plants after many years of storage is rarely reported in literature. Herein, we studied chemical profile and biological activities of Phlomis fruticosa L. after plant material storage for 20 years. Chemical analyzes were performed by UHPLC-LTQ-Orbitrap/MS, and revealed presence of 44 compounds: including 13 phenolic acids, 9 phenylethanoids, 20 flavonoids and 2 phenolic related compounds (a phenolic acid derivative and an aliphatic alcohol). The extract showed antimicrobial activity, being the most potent against Aspergillus fumigatus with minimum inhibitory concentration of 0.31 mg/mL. Also, the extract was able to inhibit biofilm formed by Candida species and to inhibit biofilm formation by Staphylococcus aureus. Obtained results revealed that the extract has potential to interfere with the cell membrane permeability of Candida albicans and to suppress production of virulence factor staphyloxanthin in S. aureus. Furthermore, the extract inhibited the activity of α-amylase which is one of the therapeutic targets for diabetes type II. Also, the antiproliferative effect of the extract was demonstrated on human cancer cell lines, while the extract did not exhibit any cytotoxic effect on primary human cells. Based on the obtained results, P. fruticosa could be an interesting source of biologically active compounds even after long term storage.
PB  - Elsevier B.V.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.
VL  - 195
DO  - 10.1016/j.jpba.2020.113884
SP  - 113884
ER  - 

@article{
author = "Stojković, Dejan and Gašić, Uroš and Drakulić, Danijela and Zengin, Gokhan and Stevanović, Milena and Rajčević, Nemanja and Soković, Marina",
year = "2021",
abstract = "Structural diversity of biologically active compounds identified in plants after many years of storage is rarely reported in literature. Herein, we studied chemical profile and biological activities of Phlomis fruticosa L. after plant material storage for 20 years. Chemical analyzes were performed by UHPLC-LTQ-Orbitrap/MS, and revealed presence of 44 compounds: including 13 phenolic acids, 9 phenylethanoids, 20 flavonoids and 2 phenolic related compounds (a phenolic acid derivative and an aliphatic alcohol). The extract showed antimicrobial activity, being the most potent against Aspergillus fumigatus with minimum inhibitory concentration of 0.31 mg/mL. Also, the extract was able to inhibit biofilm formed by Candida species and to inhibit biofilm formation by Staphylococcus aureus. Obtained results revealed that the extract has potential to interfere with the cell membrane permeability of Candida albicans and to suppress production of virulence factor staphyloxanthin in S. aureus. Furthermore, the extract inhibited the activity of α-amylase which is one of the therapeutic targets for diabetes type II. Also, the antiproliferative effect of the extract was demonstrated on human cancer cell lines, while the extract did not exhibit any cytotoxic effect on primary human cells. Based on the obtained results, P. fruticosa could be an interesting source of biologically active compounds even after long term storage.",
publisher = "Elsevier B.V.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.",
volume = "195",
doi = "10.1016/j.jpba.2020.113884",
pages = "113884"
}

Stojković, D., Gašić, U., Drakulić, D., Zengin, G., Stevanović, M., Rajčević, N.,& Soković, M.. (2021). Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier B.V.., 195, 113884.
https://doi.org/10.1016/j.jpba.2020.113884

Stojković D, Gašić U, Drakulić D, Zengin G, Stevanović M, Rajčević N, Soković M. Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.. in Journal of Pharmaceutical and Biomedical Analysis. 2021;195:113884.
doi:10.1016/j.jpba.2020.113884 .

Stojković, Dejan, Gašić, Uroš, Drakulić, Danijela, Zengin, Gokhan, Stevanović, Milena, Rajčević, Nemanja, Soković, Marina, "Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L." in Journal of Pharmaceutical and Biomedical Analysis, 195 (2021):113884,
https://doi.org/10.1016/j.jpba.2020.113884 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Schwirtlich, Marija
AU  - Rajčević, Nemanja
AU  - Stevanović, Milena
AU  - Soković, Marina
AU  - Gašić, Uroš
PY  - 2021
UR  - https://www.mdpi.com/1424-8247/14/1/55
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4209
AB  - Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.
PB  - MDPI AG
T2  - Pharmaceuticals
T1  - Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions
IS  - 1
VL  - 14
DO  - 10.3390/ph14010055
SP  - 55
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Schwirtlich, Marija and Rajčević, Nemanja and Stevanović, Milena and Soković, Marina and Gašić, Uroš",
year = "2021",
abstract = "Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.",
publisher = "MDPI AG",
journal = "Pharmaceuticals",
title = "Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions",
number = "1",
volume = "14",
doi = "10.3390/ph14010055",
pages = "55"
}

Stojković, D., Drakulić, D., Schwirtlich, M., Rajčević, N., Stevanović, M., Soković, M.,& Gašić, U.. (2021). Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions. in Pharmaceuticals
MDPI AG., 14(1), 55.
https://doi.org/10.3390/ph14010055

Stojković D, Drakulić D, Schwirtlich M, Rajčević N, Stevanović M, Soković M, Gašić U. Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions. in Pharmaceuticals. 2021;14(1):55.
doi:10.3390/ph14010055 .

Stojković, Dejan, Drakulić, Danijela, Schwirtlich, Marija, Rajčević, Nemanja, Stevanović, Milena, Soković, Marina, Gašić, Uroš, "Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions" in Pharmaceuticals, 14, no. 1 (2021):55,
https://doi.org/10.3390/ph14010055 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Dias, Maria Inês
AU  - Drakulić, Danijela
AU  - Barros, Lillian
AU  - Stevanović, Milena
AU  - C F R Ferreira, Isabel
AU  - Soković, Marina
PY  - 2020
UR  - https://www.mdpi.com/1424-8247/13/4/78
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32344670
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7243113
UR  - https://radar.ibiss.bg.ac.rs/123456789/3854
AB  - Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.
PB  - MDPI AG
T2  - Pharmaceuticals (Basel, Switzerland)
T1  - Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.
IS  - 4
VL  - 13
DO  - 10.3390/ph13040078
SP  - 78
ER  - 

@article{
author = "Stojković, Dejan and Dias, Maria Inês and Drakulić, Danijela and Barros, Lillian and Stevanović, Milena and C F R Ferreira, Isabel and Soković, Marina",
year = "2020",
abstract = "Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.",
publisher = "MDPI AG",
journal = "Pharmaceuticals (Basel, Switzerland)",
title = "Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.",
number = "4",
volume = "13",
doi = "10.3390/ph13040078",
pages = "78"
}

Stojković, D., Dias, M. I., Drakulić, D., Barros, L., Stevanović, M., C F R Ferreira, I.,& Soković, M.. (2020). Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.. in Pharmaceuticals (Basel, Switzerland)
MDPI AG., 13(4), 78.
https://doi.org/10.3390/ph13040078

Stojković D, Dias MI, Drakulić D, Barros L, Stevanović M, C F R Ferreira I, Soković M. Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.. in Pharmaceuticals (Basel, Switzerland). 2020;13(4):78.
doi:10.3390/ph13040078 .

Stojković, Dejan, Dias, Maria Inês, Drakulić, Danijela, Barros, Lillian, Stevanović, Milena, C F R Ferreira, Isabel, Soković, Marina, "Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells." in Pharmaceuticals (Basel, Switzerland), 13, no. 4 (2020):78,
https://doi.org/10.3390/ph13040078 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Gašić, Uroš
AU  - Zengin, Gokhan
AU  - Stevanović, Milena
AU  - Rajčević, Nemanja
AU  - Soković, Marina
PY  - 2020
UR  - https://pubs.rsc.org/en/content/articlehtml/2020/fo/d0fo01595d
UR  - https://pubs.rsc.org/en/content/articlelanding/2020/fo/d0fo01595d
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32749435
UR  - https://radar.ibiss.bg.ac.rs/123456789/3853
AB  - This study explored the chemical profile of the aerial parts of Ononis spinosa and further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential against Staphylococcus aureus and was able to supress the production of staphyloxanthin in S. aureus at sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.
PB  - Royal Society of Chemistry (RSC)
T2  - Food & Function
T1  - Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract
IS  - 8
VL  - 11
DO  - 10.1039/d0fo01595d
SP  - 7138
EP  - 7151
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Gašić, Uroš and Zengin, Gokhan and Stevanović, Milena and Rajčević, Nemanja and Soković, Marina",
year = "2020",
abstract = "This study explored the chemical profile of the aerial parts of Ononis spinosa and further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential against Staphylococcus aureus and was able to supress the production of staphyloxanthin in S. aureus at sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Food & Function",
title = "Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract",
number = "8",
volume = "11",
doi = "10.1039/d0fo01595d",
pages = "7138-7151"
}

Stojković, D., Drakulić, D., Gašić, U., Zengin, G., Stevanović, M., Rajčević, N.,& Soković, M.. (2020). Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract. in Food & Function
Royal Society of Chemistry (RSC)., 11(8), 7138-7151.
https://doi.org/10.1039/d0fo01595d

Stojković D, Drakulić D, Gašić U, Zengin G, Stevanović M, Rajčević N, Soković M. Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract. in Food & Function. 2020;11(8):7138-7151.
doi:10.1039/d0fo01595d .

Stojković, Dejan, Drakulić, Danijela, Gašić, Uroš, Zengin, Gokhan, Stevanović, Milena, Rajčević, Nemanja, Soković, Marina, "Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract" in Food & Function, 11, no. 8 (2020):7138-7151,
https://doi.org/10.1039/d0fo01595d . .

TY  - JOUR
AU  - Davidović, Slobodan
AU  - Malyarchuk, Boris
AU  - Grzybowski, Tomasz
AU  - Aleksić, Jelena M.
AU  - Derenko, Miroslava
AU  - Litvinov, Andrey
AU  - Rogalla-Ładniak, Urszula
AU  - Stevanović, Milena
AU  - Kovačević-Grujičić, Nataša
PY  - 2020
UR  - http://link.springer.com/10.1007/s00414-020-02324-x
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32504149
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3685
AB  - Mitochondrial genome (mtDNA) is a valuable resource in resolving various human forensic casework. The usage of variability of complete mtDNA genomes increases their discriminatory power to the maximum and enables ultimate resolution of distinct maternal lineages. However, their wider employment in forensic casework is nowadays limited by the lack of appropriate reference database. In order to fill in the gap in the reference data, which, considering Slavic-speaking populations, currently comprises only mitogenomes of East and West Slavs, we present mitogenome data for 226 Serbians, representatives of South Slavs from the Balkan Peninsula. We found 143 (sub)haplogroups among which West Eurasian ones were dominant. The percentage of unique haplotypes was 85%, and the random match probability was as low as 0.53%. We support previous findings on both high levels of genetic diversity in the Serbian population and patterns of genetic differentiation among this and ten studied European populations. However, our high-resolution data supported more pronounced genetic differentiation among Serbians and two Slavic populations (Russians and Poles) as well as expansion of the Serbian population after the Last Glacial Maximum and during the Migration period (fourth to ninth century A.D.), as inferred from the Bayesian skyline analysis. Phylogenetic analysis of haplotypes found in Serbians contributed towards the improvement of the worldwide mtDNA phylogeny, which is essential for the interpretation of the mtDNA casework.
PB  - Springer
T2  - International Journal of Legal Medicine
T1  - Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases.
VL  - 134
DO  - 10.1007/s00414-020-02324-x
SP  - 1581
EP  - 1590
ER  - 

@article{
author = "Davidović, Slobodan and Malyarchuk, Boris and Grzybowski, Tomasz and Aleksić, Jelena M. and Derenko, Miroslava and Litvinov, Andrey and Rogalla-Ładniak, Urszula and Stevanović, Milena and Kovačević-Grujičić, Nataša",
year = "2020",
abstract = "Mitochondrial genome (mtDNA) is a valuable resource in resolving various human forensic casework. The usage of variability of complete mtDNA genomes increases their discriminatory power to the maximum and enables ultimate resolution of distinct maternal lineages. However, their wider employment in forensic casework is nowadays limited by the lack of appropriate reference database. In order to fill in the gap in the reference data, which, considering Slavic-speaking populations, currently comprises only mitogenomes of East and West Slavs, we present mitogenome data for 226 Serbians, representatives of South Slavs from the Balkan Peninsula. We found 143 (sub)haplogroups among which West Eurasian ones were dominant. The percentage of unique haplotypes was 85%, and the random match probability was as low as 0.53%. We support previous findings on both high levels of genetic diversity in the Serbian population and patterns of genetic differentiation among this and ten studied European populations. However, our high-resolution data supported more pronounced genetic differentiation among Serbians and two Slavic populations (Russians and Poles) as well as expansion of the Serbian population after the Last Glacial Maximum and during the Migration period (fourth to ninth century A.D.), as inferred from the Bayesian skyline analysis. Phylogenetic analysis of haplotypes found in Serbians contributed towards the improvement of the worldwide mtDNA phylogeny, which is essential for the interpretation of the mtDNA casework.",
publisher = "Springer",
journal = "International Journal of Legal Medicine",
title = "Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases.",
volume = "134",
doi = "10.1007/s00414-020-02324-x",
pages = "1581-1590"
}

Davidović, S., Malyarchuk, B., Grzybowski, T., Aleksić, J. M., Derenko, M., Litvinov, A., Rogalla-Ładniak, U., Stevanović, M.,& Kovačević-Grujičić, N.. (2020). Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases.. in International Journal of Legal Medicine
Springer., 134, 1581-1590.
https://doi.org/10.1007/s00414-020-02324-x

Davidović S, Malyarchuk B, Grzybowski T, Aleksić JM, Derenko M, Litvinov A, Rogalla-Ładniak U, Stevanović M, Kovačević-Grujičić N. Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases.. in International Journal of Legal Medicine. 2020;134:1581-1590.
doi:10.1007/s00414-020-02324-x .

Davidović, Slobodan, Malyarchuk, Boris, Grzybowski, Tomasz, Aleksić, Jelena M., Derenko, Miroslava, Litvinov, Andrey, Rogalla-Ładniak, Urszula, Stevanović, Milena, Kovačević-Grujičić, Nataša, "Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases." in International Journal of Legal Medicine, 134 (2020):1581-1590,
https://doi.org/10.1007/s00414-020-02324-x . .

TY  - CONF
AU  - Drakulić, Danijela
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Stevanović, Milena
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4370
AB  - Cancer represents a heterogeneous group of diseases characterized by uncontrolled 
proliferation of cells, tissue invasion and metastasis. Despite current therapeutic 
strategies which include a combination of surgery, radiation and chemotherapy, cancer 
is second leading cause of death worldwide next to cardiovascular diseases. Therefore, 
the identification of new drugs with high sensitivity to cancer cells is warranted. The 
anticancer properties of plants and their compounds have been shown since ancient 
times and nowadays, there is a growing interest for identification of new plant-derived 
anticancer agents. 
This study was designed to analyze antiproliferative potential of three different plant 
species, namely Ononis spinosa L. (Fabaceae), Anthriscus cerefolium (L.) Hoffm. (Apiaceae) 
and Phlomis fruticosa L. (Lamiaceae). The aerial parts of plants were collected, dried 
and the plant material was subjected to successive extraction with methanol and 
then evaporated to dryness on a rotary vacuum evaporator. The obtained methanolic 
extracts were used for further analysis. Cytotoxic effect of methanolic extracts on HaCaT 
(spontaneously immortalized human keratinocyte cell line), MCF-7 (breast cancer cell 
line), SiHa (cervical cancer cell line), A172 (glioblastoma cell line) and HepG2 (human 
hepatocellular carcinoma cell line) cells was determined by crystal violet colorimetric 
assay. Antiproliferative activity of methanolic extracts on glioblastoma cells was 
evaluated by Ki67 immunostaining.
The results showed that plant extracts reduced viability of all tested cell lines. The 
most active extract was the one from O. spinosa, indicating by the lowest IC50 value. 
Ki67 immunostaining indicates antiproliferative activity of methanolic extracts on 
glioblastoma cells.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade
C3  - The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
T1  - Comparative study of antiproliferative potential of three  different plant species
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4370
ER  - 

@conference{
author = "Drakulić, Danijela and Stojković, Dejan and Soković, Marina and Stevanović, Milena",
year = "2019",
abstract = "Cancer represents a heterogeneous group of diseases characterized by uncontrolled 
proliferation of cells, tissue invasion and metastasis. Despite current therapeutic 
strategies which include a combination of surgery, radiation and chemotherapy, cancer 
is second leading cause of death worldwide next to cardiovascular diseases. Therefore, 
the identification of new drugs with high sensitivity to cancer cells is warranted. The 
anticancer properties of plants and their compounds have been shown since ancient 
times and nowadays, there is a growing interest for identification of new plant-derived 
anticancer agents. 
This study was designed to analyze antiproliferative potential of three different plant 
species, namely Ononis spinosa L. (Fabaceae), Anthriscus cerefolium (L.) Hoffm. (Apiaceae) 
and Phlomis fruticosa L. (Lamiaceae). The aerial parts of plants were collected, dried 
and the plant material was subjected to successive extraction with methanol and 
then evaporated to dryness on a rotary vacuum evaporator. The obtained methanolic 
extracts were used for further analysis. Cytotoxic effect of methanolic extracts on HaCaT 
(spontaneously immortalized human keratinocyte cell line), MCF-7 (breast cancer cell 
line), SiHa (cervical cancer cell line), A172 (glioblastoma cell line) and HepG2 (human 
hepatocellular carcinoma cell line) cells was determined by crystal violet colorimetric 
assay. Antiproliferative activity of methanolic extracts on glioblastoma cells was 
evaluated by Ki67 immunostaining.
The results showed that plant extracts reduced viability of all tested cell lines. The 
most active extract was the one from O. spinosa, indicating by the lowest IC50 value. 
Ki67 immunostaining indicates antiproliferative activity of methanolic extracts on 
glioblastoma cells.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia",
title = "Comparative study of antiproliferative potential of three  different plant species",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4370"
}

Drakulić, D., Stojković, D., Soković, M.,& Stevanović, M.. (2019). Comparative study of antiproliferative potential of three  different plant species. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_4370

Drakulić D, Stojković D, Soković M, Stevanović M. Comparative study of antiproliferative potential of three  different plant species. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia. 2019;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_4370 .

Drakulić, Danijela, Stojković, Dejan, Soković, Marina, Stevanović, Milena, "Comparative study of antiproliferative potential of three  different plant species" in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia (2019):38,
https://hdl.handle.net/21.15107/rcub_ibiss_4370 .

TY  - CHAP
AU  - Stojković, Dejan
AU  - Živković, Jelena
AU  - Stanisavljević, Danijela
AU  - Ivanov, Marija
AU  - Popović, Jelena
AU  - Stevanović, Milena
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2019
UR  - https://www.eurekaselect.com/176849/chapter/cytotoxicity-through-molecular-targets-involved-in-apoptosis.-where-should-we-further-search-for-mushrooms-functi
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3964
AB  - Apoptosis is considered as a classical way of programmed cell death and important control mechanism of cell homeostasis. Cancer cells acquire different instruments to circumvent programmed cell death. This promotes uncontrolled growth and frequently confers chemoresistance to tumor cells. Activation of apoptotic signaling pathway has been a target of anti-cancer drugs in an induction of cytotoxicity. The mechanism of apoptosis is complex and includes many pathways. This chapter will focus on the current knowledge of apoptosis-triggering approaches in cancer therapy, as well as on mushroom extracts and isolated compounds acting as initiators of apoptosis through regulation of genes expression involved in cancer cell death. Furthermore, we pointed out directions for novel search for mushroom functionalities in this experimental field and discuss the possible further steps for exploration on the in vitro and in vivo levels.
PB  - Bentham Science Publishers
T2  - Frontiers in Natural Product Chemistry
T1  - Cytotoxicity Through Molecular Targets Involved in Apoptosis. Where Should We Further Search for Mushrooms Functionalities in Future Cancer Treatment?
VL  - 5
DO  - 10.2174/9789811423772119050007
SP  - 146
EP  - 191
ER  - 

@inbook{
editor = "Atta-ur-Rahman",
author = "Stojković, Dejan and Živković, Jelena and Stanisavljević, Danijela and Ivanov, Marija and Popović, Jelena and Stevanović, Milena and Glamočlija, Jasmina and Soković, Marina",
year = "2019",
abstract = "Apoptosis is considered as a classical way of programmed cell death and important control mechanism of cell homeostasis. Cancer cells acquire different instruments to circumvent programmed cell death. This promotes uncontrolled growth and frequently confers chemoresistance to tumor cells. Activation of apoptotic signaling pathway has been a target of anti-cancer drugs in an induction of cytotoxicity. The mechanism of apoptosis is complex and includes many pathways. This chapter will focus on the current knowledge of apoptosis-triggering approaches in cancer therapy, as well as on mushroom extracts and isolated compounds acting as initiators of apoptosis through regulation of genes expression involved in cancer cell death. Furthermore, we pointed out directions for novel search for mushroom functionalities in this experimental field and discuss the possible further steps for exploration on the in vitro and in vivo levels.",
publisher = "Bentham Science Publishers",
journal = "Frontiers in Natural Product Chemistry",
booktitle = "Cytotoxicity Through Molecular Targets Involved in Apoptosis. Where Should We Further Search for Mushrooms Functionalities in Future Cancer Treatment?",
volume = "5",
doi = "10.2174/9789811423772119050007",
pages = "146-191"
}

Atta-ur-Rahman, Stojković, D., Živković, J., Stanisavljević, D., Ivanov, M., Popović, J., Stevanović, M., Glamočlija, J.,& Soković, M.. (2019). Cytotoxicity Through Molecular Targets Involved in Apoptosis. Where Should We Further Search for Mushrooms Functionalities in Future Cancer Treatment?. in Frontiers in Natural Product Chemistry
Bentham Science Publishers., 5, 146-191.
https://doi.org/10.2174/9789811423772119050007

Atta-ur-Rahman, Stojković D, Živković J, Stanisavljević D, Ivanov M, Popović J, Stevanović M, Glamočlija J, Soković M. Cytotoxicity Through Molecular Targets Involved in Apoptosis. Where Should We Further Search for Mushrooms Functionalities in Future Cancer Treatment?. in Frontiers in Natural Product Chemistry. 2019;5:146-191.
doi:10.2174/9789811423772119050007 .

Atta-ur-Rahman, Stojković, Dejan, Živković, Jelena, Stanisavljević, Danijela, Ivanov, Marija, Popović, Jelena, Stevanović, Milena, Glamočlija, Jasmina, Soković, Marina, "Cytotoxicity Through Molecular Targets Involved in Apoptosis. Where Should We Further Search for Mushrooms Functionalities in Future Cancer Treatment?" in Frontiers in Natural Product Chemistry, 5 (2019):146-191,
https://doi.org/10.2174/9789811423772119050007 . .

TY  - JOUR
AU  - Aleksić, Milena
AU  - Stanisavljević, Danijela
AU  - Ivanov, Marija
AU  - Vasiljević, Perica
AU  - Stevanović, Milena
AU  - Soković, Marina
AU  - Stojković, Dejan
PY  - 2019
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12532
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3444
AB  - Pyrimethanil, a synthetic fungicide widely used for the treatment of pre‐ and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 μg μL−1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyrimethanil on human cell lines were recorded already at 50 ng μL−1, while the expression of Ki67 marker of proliferation was decreased with 150 ng μL−1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity.
T2  - Annals of Applied Biology
T1  - Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines
IS  - 2
VL  - 175
DO  - 10.1111/aab.12532
SP  - 228
EP  - 235
ER  - 

@article{
author = "Aleksić, Milena and Stanisavljević, Danijela and Ivanov, Marija and Vasiljević, Perica and Stevanović, Milena and Soković, Marina and Stojković, Dejan",
year = "2019",
abstract = "Pyrimethanil, a synthetic fungicide widely used for the treatment of pre‐ and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 μg μL−1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyrimethanil on human cell lines were recorded already at 50 ng μL−1, while the expression of Ki67 marker of proliferation was decreased with 150 ng μL−1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity.",
journal = "Annals of Applied Biology",
title = "Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines",
number = "2",
volume = "175",
doi = "10.1111/aab.12532",
pages = "228-235"
}

Aleksić, M., Stanisavljević, D., Ivanov, M., Vasiljević, P., Stevanović, M., Soković, M.,& Stojković, D.. (2019). Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines. in Annals of Applied Biology, 175(2), 228-235.
https://doi.org/10.1111/aab.12532

Aleksić M, Stanisavljević D, Ivanov M, Vasiljević P, Stevanović M, Soković M, Stojković D. Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines. in Annals of Applied Biology. 2019;175(2):228-235.
doi:10.1111/aab.12532 .

Aleksić, Milena, Stanisavljević, Danijela, Ivanov, Marija, Vasiljević, Perica, Stevanović, Milena, Soković, Marina, Stojković, Dejan, "Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines" in Annals of Applied Biology, 175, no. 2 (2019):228-235,
https://doi.org/10.1111/aab.12532 . .

TY  - CONF
AU  - Kovačević-Grujičić, Nataša
AU  - Davidović, Slobodan
AU  - Malyarchuk, Boris
AU  - Grzybowski, Tomasz
AU  - Aleksić, Jelena
AU  - Derenko, Miroslava
AU  - Litvinov, Andrey
AU  - Rogalla-Ladniak, Urszula
AU  - Stevanović, Milena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5886
AB  - Mitochondrial DNA (mtDNA) is used in forensics for over three decades and is particularly
suitable when STR profiling cannot be performed due to the degraded and/or scarce nuclear
DNA. Traditionally, mtDNA typing is based on ~600 bp of the hypervariable segments I and
II (HVS-I and HVS-II) of the control region (CR, ~1100 bp). Nowadays, it is possible to use
variability of complete mtDNAs which enables maximum resolution of distinct maternal
lineages. However, the number of complete mitogenomes in reference databases such as
EMPOP is still insufficient, and that hampers their wider usage in forensic casework. In order
to fill in the gap in the reference database, which, considering Slavic-speaking populations,
currently comprises only mitogenomes of East and West Slavs, we present population data
for 226 Serbian mitogenomes, representatives of South Slavs from the Balkan Peninsula. We
support previous findings on both high levels of genetic diversity in the Serbian population
and patterns of genetic differentiation among Serbian and ten studied European populations.
However, increased genetic differentiation was observed among Serbian and two European
populations (Russians and Poles) with our high resolution data. We demonstrate that
the inclusion of indel polymorphisms into analysis contributed towards nearly complete
resolution of mtDNA haplotypes (97.1% vs. 86.3% without indels), and that the random
match probability was as low as 0.53%. Bayesian skyline analysis of Serbian mitogenomes
revealed population expansion after the Last Glacial Maximum and during the Migration
period (IV-IX century A.D.). Phylogenetic analysis of the Serbian and relevant West Eurasian
haplotypes contributed towards the improvement of the worldwide mtDNA phylogeny to
the certain extent, which is essential for the interpretation of the mtDNA casework. Lineages
of a putative Balkan origin as well as those shared among Serbian and other European
populations were observed.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - Whole mitochondrial genome diversity in serbian population: phylogenetic and forensic aspects
SP  - 157
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5886
ER  - 

@conference{
author = "Kovačević-Grujičić, Nataša and Davidović, Slobodan and Malyarchuk, Boris and Grzybowski, Tomasz and Aleksić, Jelena and Derenko, Miroslava and Litvinov, Andrey and Rogalla-Ladniak, Urszula and Stevanović, Milena",
year = "2019",
abstract = "Mitochondrial DNA (mtDNA) is used in forensics for over three decades and is particularly
suitable when STR profiling cannot be performed due to the degraded and/or scarce nuclear
DNA. Traditionally, mtDNA typing is based on ~600 bp of the hypervariable segments I and
II (HVS-I and HVS-II) of the control region (CR, ~1100 bp). Nowadays, it is possible to use
variability of complete mtDNAs which enables maximum resolution of distinct maternal
lineages. However, the number of complete mitogenomes in reference databases such as
EMPOP is still insufficient, and that hampers their wider usage in forensic casework. In order
to fill in the gap in the reference database, which, considering Slavic-speaking populations,
currently comprises only mitogenomes of East and West Slavs, we present population data
for 226 Serbian mitogenomes, representatives of South Slavs from the Balkan Peninsula. We
support previous findings on both high levels of genetic diversity in the Serbian population
and patterns of genetic differentiation among Serbian and ten studied European populations.
However, increased genetic differentiation was observed among Serbian and two European
populations (Russians and Poles) with our high resolution data. We demonstrate that
the inclusion of indel polymorphisms into analysis contributed towards nearly complete
resolution of mtDNA haplotypes (97.1% vs. 86.3% without indels), and that the random
match probability was as low as 0.53%. Bayesian skyline analysis of Serbian mitogenomes
revealed population expansion after the Last Glacial Maximum and during the Migration
period (IV-IX century A.D.). Phylogenetic analysis of the Serbian and relevant West Eurasian
haplotypes contributed towards the improvement of the worldwide mtDNA phylogeny to
the certain extent, which is essential for the interpretation of the mtDNA casework. Lineages
of a putative Balkan origin as well as those shared among Serbian and other European
populations were observed.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "Whole mitochondrial genome diversity in serbian population: phylogenetic and forensic aspects",
pages = "157",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5886"
}

Kovačević-Grujičić, N., Davidović, S., Malyarchuk, B., Grzybowski, T., Aleksić, J., Derenko, M., Litvinov, A., Rogalla-Ladniak, U.,& Stevanović, M.. (2019). Whole mitochondrial genome diversity in serbian population: phylogenetic and forensic aspects. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society., 157.
https://hdl.handle.net/21.15107/rcub_ibiss_5886

Kovačević-Grujičić N, Davidović S, Malyarchuk B, Grzybowski T, Aleksić J, Derenko M, Litvinov A, Rogalla-Ladniak U, Stevanović M. Whole mitochondrial genome diversity in serbian population: phylogenetic and forensic aspects. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;:157.
https://hdl.handle.net/21.15107/rcub_ibiss_5886 .

Kovačević-Grujičić, Nataša, Davidović, Slobodan, Malyarchuk, Boris, Grzybowski, Tomasz, Aleksić, Jelena, Derenko, Miroslava, Litvinov, Andrey, Rogalla-Ladniak, Urszula, Stevanović, Milena, "Whole mitochondrial genome diversity in serbian population: phylogenetic and forensic aspects" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019):157,
https://hdl.handle.net/21.15107/rcub_ibiss_5886 .

TY  - JOUR
AU  - Zaletel, Ivan
AU  - Schwirtlich, Marija
AU  - Perović, Milka
AU  - Jovanović, Mirna
AU  - Stevanović, Milena
AU  - Kanazir, Selma
AU  - Puškaš, Nela
PY  - 2018
UR  - http://www.medra.org/servlet/aliasResolver?alias=iospress&doi=10.3233/JAD-180277
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3149
AB  - Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology. Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.
T2  - Journal of Alzheimer's Disease : JAD
T1  - Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.
IS  - 3
VL  - 65
DO  - 10.3233/JAD-180277
SP  - 963
EP  - 976
ER  - 

@article{
author = "Zaletel, Ivan and Schwirtlich, Marija and Perović, Milka and Jovanović, Mirna and Stevanović, Milena and Kanazir, Selma and Puškaš, Nela",
year = "2018",
abstract = "Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology. Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.",
journal = "Journal of Alzheimer's Disease : JAD",
title = "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.",
number = "3",
volume = "65",
doi = "10.3233/JAD-180277",
pages = "963-976"
}

Zaletel, I., Schwirtlich, M., Perović, M., Jovanović, M., Stevanović, M., Kanazir, S.,& Puškaš, N.. (2018). Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.. in Journal of Alzheimer's Disease : JAD, 65(3), 963-976.
https://doi.org/10.3233/JAD-180277

Zaletel I, Schwirtlich M, Perović M, Jovanović M, Stevanović M, Kanazir S, Puškaš N. Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.. in Journal of Alzheimer's Disease : JAD. 2018;65(3):963-976.
doi:10.3233/JAD-180277 .

Zaletel, Ivan, Schwirtlich, Marija, Perović, Milka, Jovanović, Mirna, Stevanović, Milena, Kanazir, Selma, Puškaš, Nela, "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors." in Journal of Alzheimer's Disease : JAD, 65, no. 3 (2018):963-976,
https://doi.org/10.3233/JAD-180277 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Stanisavljević, Danijela
AU  - Stojković, Dejan
AU  - Petrović, Isidora
AU  - Vićentić Marjanović, Jelena
AU  - Popović, Jelena
AU  - Golić Grdadolnik, Simona
AU  - Marković, Dejan
AU  - Sanković-Babić, Snežana
AU  - Glamočlija, Jasmina
AU  - Stevanović, Milena
AU  - Soković, Marina
PY  - 2017
UR  - http://www.excli.de/vol16/Sokovic_23052017_proof.pdf
UR  - http://www.excli.de/volume16.php
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2791
AB  - Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra- and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell’s viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.
T2  - EXCLI Journal
T1  - Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions
VL  - 16
DO  - 10.17179/excli2017-300
SP  - 795
EP  - 807
ER  - 

@article{
author = "Ivanov, Marija and Stanisavljević, Danijela and Stojković, Dejan and Petrović, Isidora and Vićentić Marjanović, Jelena and Popović, Jelena and Golić Grdadolnik, Simona and Marković, Dejan and Sanković-Babić, Snežana and Glamočlija, Jasmina and Stevanović, Milena and Soković, Marina",
year = "2017",
abstract = "Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra- and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell’s viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.",
journal = "EXCLI Journal",
title = "Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions",
volume = "16",
doi = "10.17179/excli2017-300",
pages = "795-807"
}

Ivanov, M., Stanisavljević, D., Stojković, D., Petrović, I., Vićentić Marjanović, J., Popović, J., Golić Grdadolnik, S., Marković, D., Sanković-Babić, S., Glamočlija, J., Stevanović, M.,& Soković, M.. (2017). Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal, 16, 795-807.
https://doi.org/10.17179/excli2017-300

Ivanov M, Stanisavljević D, Stojković D, Petrović I, Vićentić Marjanović J, Popović J, Golić Grdadolnik S, Marković D, Sanković-Babić S, Glamočlija J, Stevanović M, Soković M. Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal. 2017;16:795-807.
doi:10.17179/excli2017-300 .

Ivanov, Marija, Stanisavljević, Danijela, Stojković, Dejan, Petrović, Isidora, Vićentić Marjanović, Jelena, Popović, Jelena, Golić Grdadolnik, Simona, Marković, Dejan, Sanković-Babić, Snežana, Glamočlija, Jasmina, Stevanović, Milena, Soković, Marina, "Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions" in EXCLI Journal, 16 (2017):795-807,
https://doi.org/10.17179/excli2017-300 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Kovačević-Grujičić, Nataša
AU  - Reis, Filipa S.
AU  - Davidović, Slobodan
AU  - Barros, Lillian
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Pavić, Aleksandar
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C.F.R.
AU  - Soković, Marina
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0023643817300452
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2580
AB  - Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with δ- and γ-tocopherols being predominant (123.35 and 77.80 μg/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 μg/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125–5 mg/mL; MIC/MFC 0.025–0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (IC50 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.
T2  - LWT - Food Science and Technology
T1  - Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract
VL  - 79
DO  - 10.1016/j.lwt.2017.01.045
SP  - 454
EP  - 462
ER  - 

@article{
author = "Stojković, Dejan and Kovačević-Grujičić, Nataša and Reis, Filipa S. and Davidović, Slobodan and Barros, Lillian and Popović, Jelena and Petrović, Isidora and Pavić, Aleksandar and Glamočlija, Jasmina and Ćirić, Ana and Stevanović, Milena and Ferreira, Isabel C.F.R. and Soković, Marina",
year = "2017",
abstract = "Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with δ- and γ-tocopherols being predominant (123.35 and 77.80 μg/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 μg/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125–5 mg/mL; MIC/MFC 0.025–0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (IC50 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.",
journal = "LWT - Food Science and Technology",
title = "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract",
volume = "79",
doi = "10.1016/j.lwt.2017.01.045",
pages = "454-462"
}

Stojković, D., Kovačević-Grujičić, N., Reis, F. S., Davidović, S., Barros, L., Popović, J., Petrović, I., Pavić, A., Glamočlija, J., Ćirić, A., Stevanović, M., Ferreira, I. C.F.R.,& Soković, M.. (2017). Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in LWT - Food Science and Technology, 79, 454-462.
https://doi.org/10.1016/j.lwt.2017.01.045

Stojković D, Kovačević-Grujičić N, Reis FS, Davidović S, Barros L, Popović J, Petrović I, Pavić A, Glamočlija J, Ćirić A, Stevanović M, Ferreira IC, Soković M. Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in LWT - Food Science and Technology. 2017;79:454-462.
doi:10.1016/j.lwt.2017.01.045 .

Stojković, Dejan, Kovačević-Grujičić, Nataša, Reis, Filipa S., Davidović, Slobodan, Barros, Lillian, Popović, Jelena, Petrović, Isidora, Pavić, Aleksandar, Glamočlija, Jasmina, Ćirić, Ana, Stevanović, Milena, Ferreira, Isabel C.F.R., Soković, Marina, "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract" in LWT - Food Science and Technology, 79 (2017):454-462,
https://doi.org/10.1016/j.lwt.2017.01.045 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Davidović, Slobodan
AU  - Zivković, Jelena
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Stevanović, Milena
AU  - Ferreira, Isabel CFR
AU  - Soković, Marina
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/939
AB  - Morchella esculenta (L.) Pers. methanolic extracts, obtained from fruiting bodies growing wild in Serbia and Portugal, were screened for their antimutagenic properties and compared to protocatechuic acid, previously identified in both species. Salmonella typhimurium TA100 reversion assay was used for the antimutagenic properties. Methanolic extracts expressed important antimutagenic potency towards S. typhimurium, which was documented by index of antimutagenicity (I). A sample from Serbia expressed slightly higher antimutagenic properties with an inhibition rate of 58.7%. A sample from Portugal gave an inhibition rate of 51.7%. Protocatechuic acid had an inhibition rate I of his(+) revertants of 72.4%. Cell viability in the presence of extracts was also documented. M. esculenta samples from Serbia and Portugal possessed novel biological potential for the studied species, as well as its phenolic compound - protocatechuic acid, identified in both samples. Genotoxic effect, regarding mitotic index and chromosomal aberration score, was also assessed by using the Allium cepa L. assay. Protocatechuic acid showed the most significant decrease in mitotic index, as well as decrease in chromosomal aberration score.
T2  - Frontiers in Life Science
T1  - Comparative evaluation of antimutagenic and antimitotic effects of Morchella esculenta extracts and protocatechuic acid
IS  - 3-4
VL  - 7
SP  - 1405
EP  - 223
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_939
ER  - 

@article{
author = "Stojković, Dejan and Davidović, Slobodan and Zivković, Jelena and Glamočlija, Jasmina and Ćirić, Ana and Stevanović, Milena and Ferreira, Isabel CFR and Soković, Marina",
year = "2013",
abstract = "Morchella esculenta (L.) Pers. methanolic extracts, obtained from fruiting bodies growing wild in Serbia and Portugal, were screened for their antimutagenic properties and compared to protocatechuic acid, previously identified in both species. Salmonella typhimurium TA100 reversion assay was used for the antimutagenic properties. Methanolic extracts expressed important antimutagenic potency towards S. typhimurium, which was documented by index of antimutagenicity (I). A sample from Serbia expressed slightly higher antimutagenic properties with an inhibition rate of 58.7%. A sample from Portugal gave an inhibition rate of 51.7%. Protocatechuic acid had an inhibition rate I of his(+) revertants of 72.4%. Cell viability in the presence of extracts was also documented. M. esculenta samples from Serbia and Portugal possessed novel biological potential for the studied species, as well as its phenolic compound - protocatechuic acid, identified in both samples. Genotoxic effect, regarding mitotic index and chromosomal aberration score, was also assessed by using the Allium cepa L. assay. Protocatechuic acid showed the most significant decrease in mitotic index, as well as decrease in chromosomal aberration score.",
journal = "Frontiers in Life Science",
title = "Comparative evaluation of antimutagenic and antimitotic effects of Morchella esculenta extracts and protocatechuic acid",
number = "3-4",
volume = "7",
pages = "1405-223",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_939"
}

Stojković, D., Davidović, S., Zivković, J., Glamočlija, J., Ćirić, A., Stevanović, M., Ferreira, I. C.,& Soković, M.. (2013). Comparative evaluation of antimutagenic and antimitotic effects of Morchella esculenta extracts and protocatechuic acid. in Frontiers in Life Science, 7(3-4), 1405-223.
https://hdl.handle.net/21.15107/rcub_ibiss_939

Stojković D, Davidović S, Zivković J, Glamočlija J, Ćirić A, Stevanović M, Ferreira IC, Soković M. Comparative evaluation of antimutagenic and antimitotic effects of Morchella esculenta extracts and protocatechuic acid. in Frontiers in Life Science. 2013;7(3-4):1405-223.
https://hdl.handle.net/21.15107/rcub_ibiss_939 .

Stojković, Dejan, Davidović, Slobodan, Zivković, Jelena, Glamočlija, Jasmina, Ćirić, Ana, Stevanović, Milena, Ferreira, Isabel CFR, Soković, Marina, "Comparative evaluation of antimutagenic and antimitotic effects of Morchella esculenta extracts and protocatechuic acid" in Frontiers in Life Science, 7, no. 3-4 (2013):1405-223,
https://hdl.handle.net/21.15107/rcub_ibiss_939 .