TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Popović, Dušanka
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6507
AB  - The use of anticoagulant rodenticides (ARs) is one of the most commonly employed management methods for
pest rodents. ARs compete with vitamin K (VK) required for the synthesis of blood clotting factors in the liver,
resulting in inhibition of blood coagulation and often animal death due to hemorrhage. Besides rodents (target
species), ARs may affect non-target animal species and humans. Out of hemostasis disturbance, the effects of ARs
may be related to the inhibition of proteins that require VK for their synthesis but are not involved in the
coagulation process, to their direct cytotoxicity, and their pro-oxidant/proinflammatory activity. A survey of the
cellular and molecular mechanisms of these sublethal/asymptomatic AR effects is given in this review. Data from
field, clinical, and experimental studies are presented. Knowledge of these mechanisms might improve hazard
characterization and identification of potential ecotoxicological risks associated with ARs, contributing to a safer
use of these chemicals.
PB  - New York, NY : Elsevier Science
T2  - Comparative Biochemistry and Physiology, Part C
T1  - Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance
VL  - 277
DO  - 10.1016/j.cbpc.2024.109841
SP  - 109841
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Tucović, Dina and Kulaš, Jelena and Popović, Dušanka and Kataranovski, Dragan and Kataranovski, Milena and Mirkov, Ivana",
year = "2024",
abstract = "The use of anticoagulant rodenticides (ARs) is one of the most commonly employed management methods for
pest rodents. ARs compete with vitamin K (VK) required for the synthesis of blood clotting factors in the liver,
resulting in inhibition of blood coagulation and often animal death due to hemorrhage. Besides rodents (target
species), ARs may affect non-target animal species and humans. Out of hemostasis disturbance, the effects of ARs
may be related to the inhibition of proteins that require VK for their synthesis but are not involved in the
coagulation process, to their direct cytotoxicity, and their pro-oxidant/proinflammatory activity. A survey of the
cellular and molecular mechanisms of these sublethal/asymptomatic AR effects is given in this review. Data from
field, clinical, and experimental studies are presented. Knowledge of these mechanisms might improve hazard
characterization and identification of potential ecotoxicological risks associated with ARs, contributing to a safer
use of these chemicals.",
publisher = "New York, NY : Elsevier Science",
journal = "Comparative Biochemistry and Physiology, Part C",
title = "Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance",
volume = "277",
doi = "10.1016/j.cbpc.2024.109841",
pages = "109841"
}

Popov Aleksandrov, A., Tucović, D., Kulaš, J., Popović, D., Kataranovski, D., Kataranovski, M.,& Mirkov, I.. (2024). Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance. in Comparative Biochemistry and Physiology, Part C
New York, NY : Elsevier Science., 277, 109841.
https://doi.org/10.1016/j.cbpc.2024.109841

Popov Aleksandrov A, Tucović D, Kulaš J, Popović D, Kataranovski D, Kataranovski M, Mirkov I. Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance. in Comparative Biochemistry and Physiology, Part C. 2024;277:109841.
doi:10.1016/j.cbpc.2024.109841 .

Popov Aleksandrov, Aleksandra, Tucović, Dina, Kulaš, Jelena, Popović, Dušanka, Kataranovski, Dragan, Kataranovski, Milena, Mirkov, Ivana, "Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance" in Comparative Biochemistry and Physiology, Part C, 277 (2024):109841,
https://doi.org/10.1016/j.cbpc.2024.109841 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Malešević, Anastasija
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6305
AB  - The importance of issues associated with urban/commensal rats and mice (property damage, management costs,
and health risks) press upon research on these animals. While the demography of commensal rodents is mostly
studied, the need for understanding factors influencing their natural morbidity/mortality is also stressed. In this
respect, more attention is expected to be paid to immunity, the physiological mechanism of defense against host
survival threats (pathogens, parasites, diseases). Commensal rats and mice carry numerous pathogens that evoke
diverse immune responses. The state of immunity in commensal house mice is studied in great detail, owing to
the use of laboratory strains in biomedical research. Because commensal rats are, compared to mice, carriers
of more zoonotic agents, rats’ immunity is studied mainly in that context. Some of these zoonotic agents cause
chronic, asymptomatic infections, which justified studies of immunological mechanisms of pathogen tolerance
versus clearance regulation in rats. Occurrence of some infections in specific tissues/organs pressed upon analysis
of local/regional immune responses and/or immunopathology. A survey of immunological activity/responses in
commensal rats is given in this review, with mention of existing data in commensal mice. It should throw some
light on the factors relevant to their morbidity and lifespan, supplementing the knowledge of commensal rodent
ecology.
PB  - John Wiley and Sons
T2  - Integrative Zoology
T1  - Physiological strategies in wild rodents: immune defenses of commensal rats
DO  - 10.1111/1749-4877.12766
SP  - 1
EP  - 21
ER  - 

@article{
author = "Mirkov, Ivana and Tucović, Dina and Kulaš, Jelena and Malešević, Anastasija and Kataranovski, Dragan and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2023",
abstract = "The importance of issues associated with urban/commensal rats and mice (property damage, management costs,
and health risks) press upon research on these animals. While the demography of commensal rodents is mostly
studied, the need for understanding factors influencing their natural morbidity/mortality is also stressed. In this
respect, more attention is expected to be paid to immunity, the physiological mechanism of defense against host
survival threats (pathogens, parasites, diseases). Commensal rats and mice carry numerous pathogens that evoke
diverse immune responses. The state of immunity in commensal house mice is studied in great detail, owing to
the use of laboratory strains in biomedical research. Because commensal rats are, compared to mice, carriers
of more zoonotic agents, rats’ immunity is studied mainly in that context. Some of these zoonotic agents cause
chronic, asymptomatic infections, which justified studies of immunological mechanisms of pathogen tolerance
versus clearance regulation in rats. Occurrence of some infections in specific tissues/organs pressed upon analysis
of local/regional immune responses and/or immunopathology. A survey of immunological activity/responses in
commensal rats is given in this review, with mention of existing data in commensal mice. It should throw some
light on the factors relevant to their morbidity and lifespan, supplementing the knowledge of commensal rodent
ecology.",
publisher = "John Wiley and Sons",
journal = "Integrative Zoology",
title = "Physiological strategies in wild rodents: immune defenses of commensal rats",
doi = "10.1111/1749-4877.12766",
pages = "1-21"
}

Mirkov, I., Tucović, D., Kulaš, J., Malešević, A., Kataranovski, D., Kataranovski, M.,& Popov Aleksandrov, A.. (2023). Physiological strategies in wild rodents: immune defenses of commensal rats. in Integrative Zoology
John Wiley and Sons., 1-21.
https://doi.org/10.1111/1749-4877.12766

Mirkov I, Tucović D, Kulaš J, Malešević A, Kataranovski D, Kataranovski M, Popov Aleksandrov A. Physiological strategies in wild rodents: immune defenses of commensal rats. in Integrative Zoology. 2023;:1-21.
doi:10.1111/1749-4877.12766 .

Mirkov, Ivana, Tucović, Dina, Kulaš, Jelena, Malešević, Anastasija, Kataranovski, Dragan, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Physiological strategies in wild rodents: immune defenses of commensal rats" in Integrative Zoology (2023):1-21,
https://doi.org/10.1111/1749-4877.12766 . .

TY  - BOOK
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4781
AB  - Problematika predstavljena u ovoj publikaciji je podeljena u nekoliko celina. U prvoj celini dati su poreklo i evolucija, sistematika i opšte odlike sisara (Mammalia), posebno glodara (Rodentia), kratak prikaz faune glodara Srbije i njihova taksonomska pripadnost, bez obimnijeg prikaza bionomije pojedinih predstavnika. Posebno je dat je pregled vrsta glodara zdravstveno i ekonomski značajnih za čoveka uključujući pripadnike porodica Muridae (mišoliki glodari), Gliridae (puhovi), Cricetidae (hrčkovi) i Sciuridae (veverice), Uz svaki prikazani takson dati su relevantni podaci o njihovoj biologiji i ekologiji, sa ukazivanjem na stepen zaštićenosti pojedinih vrsta. Druga celina obrađuje epizootiološko-epidemiološki značaj glodara sa pregledom značajnijih virusnih i bakterijskih zoonoza, zoonoza izazvanih protozoama, zatim infekcija glodara saprofitnim i keratinofilnim gljivama, endoparazitskim eumetazoama (cestodama, trematodama i nematodama) i ektoparazitima, i razmatranjem faktora koji utiču na njihovu transmisiju. Treća celina se odnosi na ekonomski značaj glodara i prikazuje gubitke/štete koje glodari nanose ljudima i pojedinim životinjskim i biljnim vrstama, metode i modele za procenu štete. Četvrta celina je najobimnija i u njoj su prikazane metode za procenu brojnosti i gustine populacija mišolikih glodara, a zatim metode borbe protiv po čoveka štetnih glodara u cilju kontrole njihovih populacija. Dat je prikaz mera za kontrolu glodara koje obuhvataju 1. preventivne mere (agrotehničke, tehnološko-manipulativne, sanitarno-higijenske i građevinsko-tehničke mere, ultrazvučne i elektromagnetne talase i repelente) i 2. direktne mere (mehaničko-fizičke mere, biološke mere kontrole, genetičke metode, hemijske metode). Dat je detaljan spisak akutnih i hroničnih pesticida za glodare i fumiganata, sa mehanizmima delovanja. Osim prikaza efektivnosti, ukazano je i na ograničenja u vezi sa ovim agensima, uključujući mehanizme razvoja rezistencije na neke od hroničnih rodenticida. Posebno je obrađen uticaj rodenticida na živi svet (vrste koje nisu ciljne) uključujući toksičnost za organizme u vodenoj sredini, ptice i druge divlje životinje sa naglaskom na uticaj antikoagulantnih rodenticida na populacionu dinamiku i biodiverzitet glodara i drugih životinjskih vrsta. Dužna pažnja je posvećena toksičnosti za domaće životinje i čoveka. U vezi sa tim dati su i pristupi u ispitivanju rizika od izloženosti vrsta koje nisu cilj delovanja rodenticida. Peta celina obrađuje sprovođenje deratizacije počevši od pripreme i primene rodenticida do načina sprovođenja mera, uz opis mera zaštite pri izvođenju postupaka deratizacije i kontrole uspešnosti izvedene deratizacije.

Recenzenti publikacije su: Dr Ivo R. Savić, redovni profesor, Biološki fakultet Univerziteta u Beogradu, u penziji; Dr Srđan Stamenković, docent, Biološki fakultet Univerziteta u Beogradu; Dr Snežana Tomanović, naučni savetnik, Institut za medicinska istraživanja Univerziteta u Beogradu; Dr Vitomir Ćupić, redovni profesor, Fakultet veterinarske medicine Univerziteta u Beogradu. Sa Instituta za biološka istraživanja „Siniša Stanković“ – Instituta od nacionalnog značaja za Republiku Srbiju Univerziteta u Beogradu, Dr Ivana Mirkov, naučni savetnik; Dr Vanja Bugarski-Stanojević, viši naučni saradnik; Dr Vida Jojić, viši naučni saradnik; Dr Nada Ćosić, naučni saradnik.
PB  - Beograd: NNK Internacional
PB  - Beograd: Institut za biološka istraživanja "Siniša Stanković"
T1  - Štetni glodari: biologija, epizootiologija, ekologija i kontrola brojnosti
SP  - 1
EP  - 1058
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4781
ER  - 

@book{
author = "Kataranovski, Dragan and Kataranovski, Milena",
year = "2021",
abstract = "Problematika predstavljena u ovoj publikaciji je podeljena u nekoliko celina. U prvoj celini dati su poreklo i evolucija, sistematika i opšte odlike sisara (Mammalia), posebno glodara (Rodentia), kratak prikaz faune glodara Srbije i njihova taksonomska pripadnost, bez obimnijeg prikaza bionomije pojedinih predstavnika. Posebno je dat je pregled vrsta glodara zdravstveno i ekonomski značajnih za čoveka uključujući pripadnike porodica Muridae (mišoliki glodari), Gliridae (puhovi), Cricetidae (hrčkovi) i Sciuridae (veverice), Uz svaki prikazani takson dati su relevantni podaci o njihovoj biologiji i ekologiji, sa ukazivanjem na stepen zaštićenosti pojedinih vrsta. Druga celina obrađuje epizootiološko-epidemiološki značaj glodara sa pregledom značajnijih virusnih i bakterijskih zoonoza, zoonoza izazvanih protozoama, zatim infekcija glodara saprofitnim i keratinofilnim gljivama, endoparazitskim eumetazoama (cestodama, trematodama i nematodama) i ektoparazitima, i razmatranjem faktora koji utiču na njihovu transmisiju. Treća celina se odnosi na ekonomski značaj glodara i prikazuje gubitke/štete koje glodari nanose ljudima i pojedinim životinjskim i biljnim vrstama, metode i modele za procenu štete. Četvrta celina je najobimnija i u njoj su prikazane metode za procenu brojnosti i gustine populacija mišolikih glodara, a zatim metode borbe protiv po čoveka štetnih glodara u cilju kontrole njihovih populacija. Dat je prikaz mera za kontrolu glodara koje obuhvataju 1. preventivne mere (agrotehničke, tehnološko-manipulativne, sanitarno-higijenske i građevinsko-tehničke mere, ultrazvučne i elektromagnetne talase i repelente) i 2. direktne mere (mehaničko-fizičke mere, biološke mere kontrole, genetičke metode, hemijske metode). Dat je detaljan spisak akutnih i hroničnih pesticida za glodare i fumiganata, sa mehanizmima delovanja. Osim prikaza efektivnosti, ukazano je i na ograničenja u vezi sa ovim agensima, uključujući mehanizme razvoja rezistencije na neke od hroničnih rodenticida. Posebno je obrađen uticaj rodenticida na živi svet (vrste koje nisu ciljne) uključujući toksičnost za organizme u vodenoj sredini, ptice i druge divlje životinje sa naglaskom na uticaj antikoagulantnih rodenticida na populacionu dinamiku i biodiverzitet glodara i drugih životinjskih vrsta. Dužna pažnja je posvećena toksičnosti za domaće životinje i čoveka. U vezi sa tim dati su i pristupi u ispitivanju rizika od izloženosti vrsta koje nisu cilj delovanja rodenticida. Peta celina obrađuje sprovođenje deratizacije počevši od pripreme i primene rodenticida do načina sprovođenja mera, uz opis mera zaštite pri izvođenju postupaka deratizacije i kontrole uspešnosti izvedene deratizacije.

Recenzenti publikacije su: Dr Ivo R. Savić, redovni profesor, Biološki fakultet Univerziteta u Beogradu, u penziji; Dr Srđan Stamenković, docent, Biološki fakultet Univerziteta u Beogradu; Dr Snežana Tomanović, naučni savetnik, Institut za medicinska istraživanja Univerziteta u Beogradu; Dr Vitomir Ćupić, redovni profesor, Fakultet veterinarske medicine Univerziteta u Beogradu. Sa Instituta za biološka istraživanja „Siniša Stanković“ – Instituta od nacionalnog značaja za Republiku Srbiju Univerziteta u Beogradu, Dr Ivana Mirkov, naučni savetnik; Dr Vanja Bugarski-Stanojević, viši naučni saradnik; Dr Vida Jojić, viši naučni saradnik; Dr Nada Ćosić, naučni saradnik.",
publisher = "Beograd: NNK Internacional, Beograd: Institut za biološka istraživanja "Siniša Stanković"",
title = "Štetni glodari: biologija, epizootiologija, ekologija i kontrola brojnosti",
pages = "1-1058",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4781"
}

Kataranovski, D.,& Kataranovski, M.. (2021). Štetni glodari: biologija, epizootiologija, ekologija i kontrola brojnosti. 
Beograd: NNK Internacional., 1-1058.
https://hdl.handle.net/21.15107/rcub_ibiss_4781

Kataranovski D, Kataranovski M. Štetni glodari: biologija, epizootiologija, ekologija i kontrola brojnosti. 2021;:1-1058.
https://hdl.handle.net/21.15107/rcub_ibiss_4781 .

Kataranovski, Dragan, Kataranovski, Milena, "Štetni glodari: biologija, epizootiologija, ekologija i kontrola brojnosti" (2021):1-1058,
https://hdl.handle.net/21.15107/rcub_ibiss_4781 .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Ćirović, Duško
AU  - Ćirić, Danica
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Kataranovski, Dragan
AU  - Savić, Ivo
PY  - 2020
UR  - https://link.springer.com/article/10.1007/s42991-020-00019-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3660
AB  - The role of intraspecific karyotype variability in reproductive isolation and speciation has been widely studied. Among the 26 genera of Palaearctic mammals, the blind mole rats genus Nannospalax has the highest karyotype variability with 74 chromosomal forms (CFs). Although these CFs have been described in detail, taxonomic effects of chromosomal rearrangements are still lacking, especially among 25 recorded CFs of European N. leucodon superspecies. As genetic discrepancies for most of them are missing, we analyze nucleotide sequence polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs. Here we provide for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus and syrmiensis using 40–57-year-old archived samples from our mammalian collection and thus demonstrate the usefulness of archived/museum samples as starting material for DNA analysis. The topology of the phylogenetic tree is congruent with the traditional taxonomic separation of recent blind mole rats with high support. Diversification of N. leucodon cluster into discrete subclusters—CFs—and the extent of evolutionary divergence among them are in accordance with previous findings of complete reproductive isolation between six CFs analyzed here. Additionally, the level of evolutionary divergence among six N. leucodon CFs resembles those recorded among clearly distinct Spalax species and four proposed species of N. ehrenbergi. These facts suggest that they could be cryptic species and bring attention to their conservation and natural resource protection.
PB  - Springer
T2  - Mammalian Biology
T1  - 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)
VL  - 100
DO  - 10.1007/s42991-020-00019-9
SP  - 315
EP  - 324
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Ćirović, Duško and Ćirić, Danica and Stojković, Oliver and Veličković, Jelena and Kataranovski, Dragan and Savić, Ivo",
year = "2020",
abstract = "The role of intraspecific karyotype variability in reproductive isolation and speciation has been widely studied. Among the 26 genera of Palaearctic mammals, the blind mole rats genus Nannospalax has the highest karyotype variability with 74 chromosomal forms (CFs). Although these CFs have been described in detail, taxonomic effects of chromosomal rearrangements are still lacking, especially among 25 recorded CFs of European N. leucodon superspecies. As genetic discrepancies for most of them are missing, we analyze nucleotide sequence polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs. Here we provide for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus and syrmiensis using 40–57-year-old archived samples from our mammalian collection and thus demonstrate the usefulness of archived/museum samples as starting material for DNA analysis. The topology of the phylogenetic tree is congruent with the traditional taxonomic separation of recent blind mole rats with high support. Diversification of N. leucodon cluster into discrete subclusters—CFs—and the extent of evolutionary divergence among them are in accordance with previous findings of complete reproductive isolation between six CFs analyzed here. Additionally, the level of evolutionary divergence among six N. leucodon CFs resembles those recorded among clearly distinct Spalax species and four proposed species of N. ehrenbergi. These facts suggest that they could be cryptic species and bring attention to their conservation and natural resource protection.",
publisher = "Springer",
journal = "Mammalian Biology",
title = "16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)",
volume = "100",
doi = "10.1007/s42991-020-00019-9",
pages = "315-324"
}

Bugarski-Stanojević, V., Stamenković, G., Ćirović, D., Ćirić, D., Stojković, O., Veličković, J., Kataranovski, D.,& Savić, I.. (2020). 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae). in Mammalian Biology
Springer., 100, 315-324.
https://doi.org/10.1007/s42991-020-00019-9

Bugarski-Stanojević V, Stamenković G, Ćirović D, Ćirić D, Stojković O, Veličković J, Kataranovski D, Savić I. 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae). in Mammalian Biology. 2020;100:315-324.
doi:10.1007/s42991-020-00019-9 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Ćirović, Duško, Ćirić, Danica, Stojković, Oliver, Veličković, Jelena, Kataranovski, Dragan, Savić, Ivo, "16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)" in Mammalian Biology, 100 (2020):315-324,
https://doi.org/10.1007/s42991-020-00019-9 . .

TY  - CONF
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Ćirović, Duško
AU  - Ćirić, Danica
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Kataranovski, Dragan
AU  - Savić, Ivo
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5657
AB  - Among 26 genera of Palaearctic mammals, the genus Nannospalax has the highest karyotype
variability with 74 chromosomal forms (CFs). Taxonomic effects i.e. implications to phylogeny
and speciation process of such chromosomal variety are still lacking, especially among 25
reported CFs of South-European N. leucodon superspecies. Many cryptic species are under
serious threat of complete disappearance, with population declines in Europe. As genetic
discrepancies for the majority of them are missing, we analyzed nucleotide sequence
polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs and also
add for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus
and syrmiensis. Further, including 40-57 years old teeth we evaluate the usefulness of the
archived samples, e.g. from museums and other old collections as starting material for
phylogenetic analysis. The topology of the Bayesian Inference tree is in agreement with the
traditional taxonomic separation of recent blind mole rats. Among the three superspecies, the
genetic diversity was lowest in N. ehrenbergi (0.004-0.031), highest in N. xanthodon (0.009-
0.063) and intermediate in N. leucodon (0.008-0.055). The comparable scale of evolutionary
divergence among N. leucodon CFs and among species from the genus Spalax supports our
previous proposal that seven reproductively isolated CFs should be considered to be cryptic
species and thus protected from extinction in their natural habitat.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5657
ER  - 

@conference{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Ćirović, Duško and Ćirić, Danica and Stojković, Oliver and Veličković, Jelena and Kataranovski, Dragan and Savić, Ivo",
year = "2019",
abstract = "Among 26 genera of Palaearctic mammals, the genus Nannospalax has the highest karyotype
variability with 74 chromosomal forms (CFs). Taxonomic effects i.e. implications to phylogeny
and speciation process of such chromosomal variety are still lacking, especially among 25
reported CFs of South-European N. leucodon superspecies. Many cryptic species are under
serious threat of complete disappearance, with population declines in Europe. As genetic
discrepancies for the majority of them are missing, we analyzed nucleotide sequence
polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs and also
add for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus
and syrmiensis. Further, including 40-57 years old teeth we evaluate the usefulness of the
archived samples, e.g. from museums and other old collections as starting material for
phylogenetic analysis. The topology of the Bayesian Inference tree is in agreement with the
traditional taxonomic separation of recent blind mole rats. Among the three superspecies, the
genetic diversity was lowest in N. ehrenbergi (0.004-0.031), highest in N. xanthodon (0.009-
0.063) and intermediate in N. leucodon (0.008-0.055). The comparable scale of evolutionary
divergence among N. leucodon CFs and among species from the genus Spalax supports our
previous proposal that seven reproductively isolated CFs should be considered to be cryptic
species and thus protected from extinction in their natural habitat.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5657"
}

Bugarski-Stanojević, V., Stamenković, G., Ćirović, D., Ćirić, D., Stojković, O., Veličković, J., Kataranovski, D.,& Savić, I.. (2019). 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae). in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5657

Bugarski-Stanojević V, Stamenković G, Ćirović D, Ćirić D, Stojković O, Veličković J, Kataranovski D, Savić I. 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae). in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;.
https://hdl.handle.net/21.15107/rcub_ibiss_5657 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Ćirović, Duško, Ćirić, Danica, Stojković, Oliver, Veličković, Jelena, Kataranovski, Dragan, Savić, Ivo, "16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_5657 .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Belij-Rammerstorfer, Sandra
AU  - Mirkov, Ivana
AU  - Subota, Vesna
AU  - Kulaš, Jelena
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2018
UR  - https://www.tandfonline.com/doi/full/10.1080/15569527.2017.1328690
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2764
AB  - Purpose: The efficacy of topical dinitrochlorobenzene (DNCB) in the treatment of some skin dermatoses is based both on local and systemic effects. It is not known, however, whether it can be applied to patients receiving some other therapy associated with systemic immunomodulation. The aim of the present paper using a rat model was to examine whether oral warfarin (WF) intake, as shown by others and by us, had an immunomodulatory potential to interfere with effects of topical DNCB as systemic immunotherapy. Materials and methods: Rats received 3.5 mg/l of WF sodium in drinking water for 30 days and were thereafter skin-sensitized with 0.4% DNCB. Changes in the oxidative activity (myeloperoxidase/MPO, reduction of nitroblue tetrazolium/NBT and nitric oxide/NO production) as well as tumor necrosis factor (TNF) production by peripheral blood polymorphonuclear cells (PMN) were measured and compared with PMN from sensitized unexposed to WF rats. Results: WF intake enhanced some aspects of PMN activity (intracellular MPO activity and unstimulated NO production) as well as their responsiveness to exogenous stimulation (NBT reduction and TNF production from sensitized animals). However, WF also decreased PMN responsiveness of NO production to stimulation. WF affected NO and TNF production solely by PMN, as no effect on these activities of peripheral blood mononuclear cells was seen. Conclusion: Having in mind that polymorphonuclear leukocytes are the most abundant cell type in peripheral blood in humans, increase of basic aspects of PMN activity described in the present paper might be relevant for consideration of using WF as therapeutic modality in patients topically treated with DNCB.
T2  - Cutaneous and Ocular Toxicology
T1  - Oral warfarin affects some aspects of systemic immunomodulation with topical dinitrochlorobenzene (DNCB) in rats
IS  - 1
VL  - 37
DO  - 10.1080/15569527.2017.1328690
SP  - 29
EP  - 35
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Belij-Rammerstorfer, Sandra and Mirkov, Ivana and Subota, Vesna and Kulaš, Jelena and Kataranovski, Dragan and Kataranovski, Milena",
year = "2018",
abstract = "Purpose: The efficacy of topical dinitrochlorobenzene (DNCB) in the treatment of some skin dermatoses is based both on local and systemic effects. It is not known, however, whether it can be applied to patients receiving some other therapy associated with systemic immunomodulation. The aim of the present paper using a rat model was to examine whether oral warfarin (WF) intake, as shown by others and by us, had an immunomodulatory potential to interfere with effects of topical DNCB as systemic immunotherapy. Materials and methods: Rats received 3.5 mg/l of WF sodium in drinking water for 30 days and were thereafter skin-sensitized with 0.4% DNCB. Changes in the oxidative activity (myeloperoxidase/MPO, reduction of nitroblue tetrazolium/NBT and nitric oxide/NO production) as well as tumor necrosis factor (TNF) production by peripheral blood polymorphonuclear cells (PMN) were measured and compared with PMN from sensitized unexposed to WF rats. Results: WF intake enhanced some aspects of PMN activity (intracellular MPO activity and unstimulated NO production) as well as their responsiveness to exogenous stimulation (NBT reduction and TNF production from sensitized animals). However, WF also decreased PMN responsiveness of NO production to stimulation. WF affected NO and TNF production solely by PMN, as no effect on these activities of peripheral blood mononuclear cells was seen. Conclusion: Having in mind that polymorphonuclear leukocytes are the most abundant cell type in peripheral blood in humans, increase of basic aspects of PMN activity described in the present paper might be relevant for consideration of using WF as therapeutic modality in patients topically treated with DNCB.",
journal = "Cutaneous and Ocular Toxicology",
title = "Oral warfarin affects some aspects of systemic immunomodulation with topical dinitrochlorobenzene (DNCB) in rats",
number = "1",
volume = "37",
doi = "10.1080/15569527.2017.1328690",
pages = "29-35"
}

Popov Aleksandrov, A., Belij-Rammerstorfer, S., Mirkov, I., Subota, V., Kulaš, J., Kataranovski, D.,& Kataranovski, M.. (2018). Oral warfarin affects some aspects of systemic immunomodulation with topical dinitrochlorobenzene (DNCB) in rats. in Cutaneous and Ocular Toxicology, 37(1), 29-35.
https://doi.org/10.1080/15569527.2017.1328690

Popov Aleksandrov A, Belij-Rammerstorfer S, Mirkov I, Subota V, Kulaš J, Kataranovski D, Kataranovski M. Oral warfarin affects some aspects of systemic immunomodulation with topical dinitrochlorobenzene (DNCB) in rats. in Cutaneous and Ocular Toxicology. 2018;37(1):29-35.
doi:10.1080/15569527.2017.1328690 .

Popov Aleksandrov, Aleksandra, Belij-Rammerstorfer, Sandra, Mirkov, Ivana, Subota, Vesna, Kulaš, Jelena, Kataranovski, Dragan, Kataranovski, Milena, "Oral warfarin affects some aspects of systemic immunomodulation with topical dinitrochlorobenzene (DNCB) in rats" in Cutaneous and Ocular Toxicology, 37, no. 1 (2018):29-35,
https://doi.org/10.1080/15569527.2017.1328690 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Tucović, Dina
AU  - Demenesku, Jelena
AU  - Subota, Vesna
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S027869151830019X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2999
AB  - Warfarin is the world's most widely used anticoagulant drug. Its anticoagulant activity is based on the inhibition of the vitamin K-dependent (VKD) step in the complete synthesis of a number of blood coagulation factors that are required for normal blood coagulation. Warfarin also affects synthesis of VKD proteins not related to haemostasis including those involved in bone growth and vascular calcification. Antithrombotic activity of warfarin is considered responsible for some aspects of its anti-tumour activity of warfarin. Some aspects of activities against tumours seem not to be related to haemostasis and included effects of warfarin on non-haemostatic VKD proteins as well as those not related to VKD proteins. Inflammatory/immunomodulatory effects of warfarin indicate much broader potential of action of this drug both in physiological and pathological processes. This review provides an overview of the published data dealing with the effects of warfarin on biological processes other than haemostasis.
PB  - Pergamon
T2  - Food and Chemical Toxicology
T1  - Effects of warfarin on biological processes other than haemostasis: A review.
VL  - 113
DO  - 10.1016/j.fct.2018.01.019
SP  - 19
EP  - 32
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Ninkov, Marina and Tucović, Dina and Demenesku, Jelena and Subota, Vesna and Kataranovski, Dragan and Kataranovski, Milena",
year = "2018",
abstract = "Warfarin is the world's most widely used anticoagulant drug. Its anticoagulant activity is based on the inhibition of the vitamin K-dependent (VKD) step in the complete synthesis of a number of blood coagulation factors that are required for normal blood coagulation. Warfarin also affects synthesis of VKD proteins not related to haemostasis including those involved in bone growth and vascular calcification. Antithrombotic activity of warfarin is considered responsible for some aspects of its anti-tumour activity of warfarin. Some aspects of activities against tumours seem not to be related to haemostasis and included effects of warfarin on non-haemostatic VKD proteins as well as those not related to VKD proteins. Inflammatory/immunomodulatory effects of warfarin indicate much broader potential of action of this drug both in physiological and pathological processes. This review provides an overview of the published data dealing with the effects of warfarin on biological processes other than haemostasis.",
publisher = "Pergamon",
journal = "Food and Chemical Toxicology",
title = "Effects of warfarin on biological processes other than haemostasis: A review.",
volume = "113",
doi = "10.1016/j.fct.2018.01.019",
pages = "19-32"
}

Popov Aleksandrov, A., Mirkov, I., Ninkov, M., Tucović, D., Demenesku, J., Subota, V., Kataranovski, D.,& Kataranovski, M.. (2018). Effects of warfarin on biological processes other than haemostasis: A review.. in Food and Chemical Toxicology
Pergamon., 113, 19-32.
https://doi.org/10.1016/j.fct.2018.01.019

Popov Aleksandrov A, Mirkov I, Ninkov M, Tucović D, Demenesku J, Subota V, Kataranovski D, Kataranovski M. Effects of warfarin on biological processes other than haemostasis: A review.. in Food and Chemical Toxicology. 2018;113:19-32.
doi:10.1016/j.fct.2018.01.019 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Ninkov, Marina, Tucović, Dina, Demenesku, Jelena, Subota, Vesna, Kataranovski, Dragan, Kataranovski, Milena, "Effects of warfarin on biological processes other than haemostasis: A review." in Food and Chemical Toxicology, 113 (2018):19-32,
https://doi.org/10.1016/j.fct.2018.01.019 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Subota, Vesna
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2018
UR  - http://doi.wiley.com/10.1111/1749-4877.12296
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29168613
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3021
AB  - Studies of wild animals' immunity often use comparison with laboratory-raised individuals. Using such an approach, various data were obtained concerning wild Norway rat's immunity. Lower or higher potential of immune system cells to respond to activation stimuli were shown, because of analysis of disparate parameters and/ or small number of analyzed individuals. Inconsistent differences between laboratory and wild rats were shown too, owing to great response variability in wild rats. We hypothesized that wild rats will express more intense immune activity compared to their laboratory counterparts which live in a less demanding environment. To test this, we analyzed the circulating levels of inflammatory cytokine interleukin-6 (IL-6), a mediator which has a central role in host immune defense. In addition, we examined the activity of the central immune organ, the spleen, including cell proliferation and production of pro-inflammatory cytokines interferon-γ (IFN-γ) and interleukin-17 (IL-17), which are major effectors of cellular adaptive immune response. In order to obtain reasonable insight into the immunity of wild Norway rats, analysis was conducted on a much larger number of individuals compared to other studies. Higher levels of plasma IL-6, higher spleen mass, cellularity and basal IFN-γ production concomitantly with lower basal production of anti-inflammatory cytokine interleukin-10 (IL-10) revealed more intense immune activity in the wild compared to laboratory rats. However, lower responsiveness of their spleen cells' proinflammatory cytokine production to concanavalin A (ConA) stimulation, along with preserved capacity of IL-10 response, might be perceived as an indication of wild rats' reduced capability to cope with incoming environmental stimuli, but also as a means to limit tissue damage.
T2  - Integrative Zoology
T1  - Immune defense of wild-caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation.
IS  - 2
VL  - 13
DO  - 10.1111/1749-4877.12296
SP  - 180
EP  - 193
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Subota, Vesna and Kataranovski, Dragan and Kataranovski, Milena",
year = "2018",
abstract = "Studies of wild animals' immunity often use comparison with laboratory-raised individuals. Using such an approach, various data were obtained concerning wild Norway rat's immunity. Lower or higher potential of immune system cells to respond to activation stimuli were shown, because of analysis of disparate parameters and/ or small number of analyzed individuals. Inconsistent differences between laboratory and wild rats were shown too, owing to great response variability in wild rats. We hypothesized that wild rats will express more intense immune activity compared to their laboratory counterparts which live in a less demanding environment. To test this, we analyzed the circulating levels of inflammatory cytokine interleukin-6 (IL-6), a mediator which has a central role in host immune defense. In addition, we examined the activity of the central immune organ, the spleen, including cell proliferation and production of pro-inflammatory cytokines interferon-γ (IFN-γ) and interleukin-17 (IL-17), which are major effectors of cellular adaptive immune response. In order to obtain reasonable insight into the immunity of wild Norway rats, analysis was conducted on a much larger number of individuals compared to other studies. Higher levels of plasma IL-6, higher spleen mass, cellularity and basal IFN-γ production concomitantly with lower basal production of anti-inflammatory cytokine interleukin-10 (IL-10) revealed more intense immune activity in the wild compared to laboratory rats. However, lower responsiveness of their spleen cells' proinflammatory cytokine production to concanavalin A (ConA) stimulation, along with preserved capacity of IL-10 response, might be perceived as an indication of wild rats' reduced capability to cope with incoming environmental stimuli, but also as a means to limit tissue damage.",
journal = "Integrative Zoology",
title = "Immune defense of wild-caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation.",
number = "2",
volume = "13",
doi = "10.1111/1749-4877.12296",
pages = "180-193"
}

Mirkov, I., Popov Aleksandrov, A., Subota, V., Kataranovski, D.,& Kataranovski, M.. (2018). Immune defense of wild-caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation.. in Integrative Zoology, 13(2), 180-193.
https://doi.org/10.1111/1749-4877.12296

Mirkov I, Popov Aleksandrov A, Subota V, Kataranovski D, Kataranovski M. Immune defense of wild-caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation.. in Integrative Zoology. 2018;13(2):180-193.
doi:10.1111/1749-4877.12296 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Subota, Vesna, Kataranovski, Dragan, Kataranovski, Milena, "Immune defense of wild-caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation." in Integrative Zoology, 13, no. 2 (2018):180-193,
https://doi.org/10.1111/1749-4877.12296 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Tucović, Dina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2017
UR  - https://www.tandfonline.com/doi/full/10.1080/15569527.2016.1275664
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2555
AB  - Purpose: Warfarin (WF) is an anticoagulant which also affects physiological processes other than hemostasis. Our previous investigations showed the effect of WF which gained access to the organism via skin on resting peripheral blood granulocytes. Based on these data, the aim of the present study was to examine whether WF could modulate the inflammatory processes as well. To this aim the effect of WF on the inflammatory response induced by subcutaneous sponge implantation in rats was examined. Materials and methods: Warfarin-soaked polyvinyl sponges (WF-sponges) were implanted subcutaneously and cell infiltration into sponges, the levels of nitric oxide (NO) and inflammatory cytokines tumor necrosis factor (TNF) and interleukin-6 (IL-6) production by sponge cells were measured as parameters of inflammation. T cell infiltration and cytokine interferon-γ (IFN-γ), interleukin-17 (IL-17) and interleukin-10 (IL-10) were measured at day 7 post implantation. Results: Warfarin exerted both stimulatory and suppressive effects depending on the parameter examined. Flow cytometry of cells recovered from sponges showed higher numbers of granulocytes (HIS48+ cells) at days 1 and 3 post implantation and CD11b+ cells at day 1 compared to control sponges. Cells from WF-sponges had an increased NO production (Griess reaction) at days 1 and 7. In contrast, lower levels of TNF (measured by ELISA) production by cells recovered from WF-soaked sponges were found in the early (day one) phase of reaction with unchanged levels at other time points. While IL-6 production by cells recovered from WF-soaked sponges was decreased at day 1, it was increased at day 7. Higher T cell numbers were noted in WF sponges at day 7 post implantation, and recovered cells produced more IFN-γ and IL-17, while IL-10 production remained unchanged. Conclusions: Warfarin affects some of the parameters of inflammatory reaction induced by subcutaneous polyvinyl sponge implantation. Differential (both stimulatory as well as inhibitory) effects of WF on inflammatory response to sponge implants might affect the course and/or duration of this reaction.
T2  - Cutaneous and Ocular Toxicology
T1  - Warfarin affects acute inflammatory response induced by subcutaneous polyvinyl sponge implantation in rats
DO  - 10.1080/15569527.2016.1275664
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Ninkov, Marina and Tucović, Dina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2017",
abstract = "Purpose: Warfarin (WF) is an anticoagulant which also affects physiological processes other than hemostasis. Our previous investigations showed the effect of WF which gained access to the organism via skin on resting peripheral blood granulocytes. Based on these data, the aim of the present study was to examine whether WF could modulate the inflammatory processes as well. To this aim the effect of WF on the inflammatory response induced by subcutaneous sponge implantation in rats was examined. Materials and methods: Warfarin-soaked polyvinyl sponges (WF-sponges) were implanted subcutaneously and cell infiltration into sponges, the levels of nitric oxide (NO) and inflammatory cytokines tumor necrosis factor (TNF) and interleukin-6 (IL-6) production by sponge cells were measured as parameters of inflammation. T cell infiltration and cytokine interferon-γ (IFN-γ), interleukin-17 (IL-17) and interleukin-10 (IL-10) were measured at day 7 post implantation. Results: Warfarin exerted both stimulatory and suppressive effects depending on the parameter examined. Flow cytometry of cells recovered from sponges showed higher numbers of granulocytes (HIS48+ cells) at days 1 and 3 post implantation and CD11b+ cells at day 1 compared to control sponges. Cells from WF-sponges had an increased NO production (Griess reaction) at days 1 and 7. In contrast, lower levels of TNF (measured by ELISA) production by cells recovered from WF-soaked sponges were found in the early (day one) phase of reaction with unchanged levels at other time points. While IL-6 production by cells recovered from WF-soaked sponges was decreased at day 1, it was increased at day 7. Higher T cell numbers were noted in WF sponges at day 7 post implantation, and recovered cells produced more IFN-γ and IL-17, while IL-10 production remained unchanged. Conclusions: Warfarin affects some of the parameters of inflammatory reaction induced by subcutaneous polyvinyl sponge implantation. Differential (both stimulatory as well as inhibitory) effects of WF on inflammatory response to sponge implants might affect the course and/or duration of this reaction.",
journal = "Cutaneous and Ocular Toxicology",
title = "Warfarin affects acute inflammatory response induced by subcutaneous polyvinyl sponge implantation in rats",
doi = "10.1080/15569527.2016.1275664"
}

Mirkov, I., Popov Aleksandrov, A., Demenesku, J., Ninkov, M., Tucović, D., Kataranovski, D.,& Kataranovski, M.. (2017). Warfarin affects acute inflammatory response induced by subcutaneous polyvinyl sponge implantation in rats. in Cutaneous and Ocular Toxicology.
https://doi.org/10.1080/15569527.2016.1275664

Mirkov I, Popov Aleksandrov A, Demenesku J, Ninkov M, Tucović D, Kataranovski D, Kataranovski M. Warfarin affects acute inflammatory response induced by subcutaneous polyvinyl sponge implantation in rats. in Cutaneous and Ocular Toxicology. 2017;.
doi:10.1080/15569527.2016.1275664 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Ninkov, Marina, Tucović, Dina, Kataranovski, Dragan, Kataranovski, Milena, "Warfarin affects acute inflammatory response induced by subcutaneous polyvinyl sponge implantation in rats" in Cutaneous and Ocular Toxicology (2017),
https://doi.org/10.1080/15569527.2016.1275664 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Zolotarevski, Lidija
AU  - Ninkov, Marina
AU  - Tucović, Dina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2017
UR  - https://www.sciencedirect.com/science/article/pii/S1382668917301746?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3540
AB  - Warfarin is an anticoagulant used in prevention/prophylaxis of thromboembolism. Besides the effects on coagulation, non-hemorrhagic reactions have also been documented. Although cutaneous reactions were reported in some patients, the impact on skin immunity was not explored. In the present paper, the effect of 30-day oral warfarin intake on skin cytokine responses in rats was analyzed. Increased release of inflammatory cytokines (TNF, IL-1β and IL-10) was noted by skin explants from rats which received warfarin, but without effect on IL-6. No impact on epidermal cell cytokine secretion was seen, except a tendency of an increase of IL-6 response to stimulation with microbial product lipopolysaccharide (LPS). Topical application of contact allergen dinitrochlorobenzene (DNCB) resulted in slight (numerical solely) increase of TNF release by skin explants of warfarin-treated animals, while epidermal cells responded by increased secretion of all four cytokines examined. The data presented provide new information on the potential of oral warfarin to modulate skin innate immune activity.
T2  - Environmental Toxicology and Pharmacology
T1  - Oral warfarin intake affects skin inflammatory cytokine responses in rats.
VL  - 54
DO  - 10.1016/j.etap.2017.06.027
SP  - 93
EP  - 98
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Zolotarevski, Lidija and Ninkov, Marina and Tucović, Dina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2017",
abstract = "Warfarin is an anticoagulant used in prevention/prophylaxis of thromboembolism. Besides the effects on coagulation, non-hemorrhagic reactions have also been documented. Although cutaneous reactions were reported in some patients, the impact on skin immunity was not explored. In the present paper, the effect of 30-day oral warfarin intake on skin cytokine responses in rats was analyzed. Increased release of inflammatory cytokines (TNF, IL-1β and IL-10) was noted by skin explants from rats which received warfarin, but without effect on IL-6. No impact on epidermal cell cytokine secretion was seen, except a tendency of an increase of IL-6 response to stimulation with microbial product lipopolysaccharide (LPS). Topical application of contact allergen dinitrochlorobenzene (DNCB) resulted in slight (numerical solely) increase of TNF release by skin explants of warfarin-treated animals, while epidermal cells responded by increased secretion of all four cytokines examined. The data presented provide new information on the potential of oral warfarin to modulate skin innate immune activity.",
journal = "Environmental Toxicology and Pharmacology",
title = "Oral warfarin intake affects skin inflammatory cytokine responses in rats.",
volume = "54",
doi = "10.1016/j.etap.2017.06.027",
pages = "93-98"
}

Popov Aleksandrov, A., Mirkov, I., Zolotarevski, L., Ninkov, M., Tucović, D., Kataranovski, D.,& Kataranovski, M.. (2017). Oral warfarin intake affects skin inflammatory cytokine responses in rats.. in Environmental Toxicology and Pharmacology, 54, 93-98.
https://doi.org/10.1016/j.etap.2017.06.027

Popov Aleksandrov A, Mirkov I, Zolotarevski L, Ninkov M, Tucović D, Kataranovski D, Kataranovski M. Oral warfarin intake affects skin inflammatory cytokine responses in rats.. in Environmental Toxicology and Pharmacology. 2017;54:93-98.
doi:10.1016/j.etap.2017.06.027 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Zolotarevski, Lidija, Ninkov, Marina, Tucović, Dina, Kataranovski, Dragan, Kataranovski, Milena, "Oral warfarin intake affects skin inflammatory cytokine responses in rats." in Environmental Toxicology and Pharmacology, 54 (2017):93-98,
https://doi.org/10.1016/j.etap.2017.06.027 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Demenesku, Jelena
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Stefik, Debora
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4813
AB  - Intestinal hemorrhage characterizes effectiveness of warfarin (WF) as rodenticide and is amongadverse effects of therapy in humans. Having in mind genetic variations in the effectiveness ofWF in wild rats and in the doses required for therapeutic effect, strain differences in the intestinaltoxicity of oral warfarin in rats were examined in this study. High WF dose (3.5 mg/l) led tomortality in Albino Oxford (AO) rats, with no lethality in Dark Agouti (DA) rats. Higher valuesof prothrombin time were noted at low WF dose (0.35 mg/l) in the former strain. Leukocyteinfiltration in intestine noted at this dose in both strains was associated with oxidative injury andmore pronounced anti-oxidative response in AO rats. Suppression of mesenteric lymph node cellproliferation and IFN-γ and IL-10 production in AO rats and lack of these effects in DA rats,represent different strategies to protect vulnerable intestine from harmful immune responses.
PB  - Amsterdam, Holland:Elsevier B.V.
T2  - Environmental Toxicology and Pharmacology
T1  - Strain differences in intestinal toxicity of warfarin in rats
VL  - 48
DO  - 10.1016/j.etap.2016.10.019
SP  - 175
EP  - 182
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Mileusnić, Dina and Demenesku, Jelena and Zolotarevski, Lidija and Subota, Vesna and Stefik, Debora and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Intestinal hemorrhage characterizes effectiveness of warfarin (WF) as rodenticide and is amongadverse effects of therapy in humans. Having in mind genetic variations in the effectiveness ofWF in wild rats and in the doses required for therapeutic effect, strain differences in the intestinaltoxicity of oral warfarin in rats were examined in this study. High WF dose (3.5 mg/l) led tomortality in Albino Oxford (AO) rats, with no lethality in Dark Agouti (DA) rats. Higher valuesof prothrombin time were noted at low WF dose (0.35 mg/l) in the former strain. Leukocyteinfiltration in intestine noted at this dose in both strains was associated with oxidative injury andmore pronounced anti-oxidative response in AO rats. Suppression of mesenteric lymph node cellproliferation and IFN-γ and IL-10 production in AO rats and lack of these effects in DA rats,represent different strategies to protect vulnerable intestine from harmful immune responses.",
publisher = "Amsterdam, Holland:Elsevier B.V.",
journal = "Environmental Toxicology and Pharmacology",
title = "Strain differences in intestinal toxicity of warfarin in rats",
volume = "48",
doi = "10.1016/j.etap.2016.10.019",
pages = "175-182"
}

Mirkov, I., Popov Aleksandrov, A., Ninkov, M., Mileusnić, D., Demenesku, J., Zolotarevski, L., Subota, V., Stefik, D., Kataranovski, D.,& Kataranovski, M.. (2016). Strain differences in intestinal toxicity of warfarin in rats. in Environmental Toxicology and Pharmacology
Amsterdam, Holland:Elsevier B.V.., 48, 175-182.
https://doi.org/10.1016/j.etap.2016.10.019

Mirkov I, Popov Aleksandrov A, Ninkov M, Mileusnić D, Demenesku J, Zolotarevski L, Subota V, Stefik D, Kataranovski D, Kataranovski M. Strain differences in intestinal toxicity of warfarin in rats. in Environmental Toxicology and Pharmacology. 2016;48:175-182.
doi:10.1016/j.etap.2016.10.019 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Mileusnić, Dina, Demenesku, Jelena, Zolotarevski, Lidija, Subota, Vesna, Stefik, Debora, Kataranovski, Dragan, Kataranovski, Milena, "Strain differences in intestinal toxicity of warfarin in rats" in Environmental Toxicology and Pharmacology, 48 (2016):175-182,
https://doi.org/10.1016/j.etap.2016.10.019 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Demenesku, Jelena
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Stefik, Debora
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4812
AB  - Intestinal hemorrhage characterizes effectiveness of warfarin (WF) as rodenticide and is among
adverse effects of therapy in humans. Having in mind genetic variations in the effectiveness of
WF in wild rats and in the doses required for therapeutic effect, strain differences in the intestinal
toxicity of oral warfarin in rats were examined in this study. High WF dose (3.5 mg/l) led to
mortality in Albino Oxford (AO) rats, with no lethality in Dark Agouti (DA) rats. Higher values
of prothrombin time were noted at low WF dose (0.35 mg/l) in the former strain. Leukocyte
infiltration in intestine noted at this dose in both strains was associated with oxidative injury and
more pronounced anti-oxidative response in AO rats. Suppression of mesenteric lymph node cell
proliferation and IFN-γ and IL-10 production in AO rats and lack of these effects in DA rats,
represent different strategies to protect vulnerable intestine from harmful immune responses.
PB  - Amsterdam, Holland:Elsevier B.V.
T2  - Environmental Toxicology and Pharmacology
T1  - Strain differences in intestinal toxicity of warfarin in rats
VL  - 48
DO  - 10.1016/j.etap.2016.10.019
SP  - 175
EP  - 182
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Mileusnić, Dina and Demenesku, Jelena and Zolotarevski, Lidija and Subota, Vesna and Stefik, Debora and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Intestinal hemorrhage characterizes effectiveness of warfarin (WF) as rodenticide and is among
adverse effects of therapy in humans. Having in mind genetic variations in the effectiveness of
WF in wild rats and in the doses required for therapeutic effect, strain differences in the intestinal
toxicity of oral warfarin in rats were examined in this study. High WF dose (3.5 mg/l) led to
mortality in Albino Oxford (AO) rats, with no lethality in Dark Agouti (DA) rats. Higher values
of prothrombin time were noted at low WF dose (0.35 mg/l) in the former strain. Leukocyte
infiltration in intestine noted at this dose in both strains was associated with oxidative injury and
more pronounced anti-oxidative response in AO rats. Suppression of mesenteric lymph node cell
proliferation and IFN-γ and IL-10 production in AO rats and lack of these effects in DA rats,
represent different strategies to protect vulnerable intestine from harmful immune responses.",
publisher = "Amsterdam, Holland:Elsevier B.V.",
journal = "Environmental Toxicology and Pharmacology",
title = "Strain differences in intestinal toxicity of warfarin in rats",
volume = "48",
doi = "10.1016/j.etap.2016.10.019",
pages = "175-182"
}

Mirkov, I., Popov Aleksandrov, A., Ninkov, M., Mileusnić, D., Demenesku, J., Zolotarevski, L., Subota, V., Stefik, D., Kataranovski, D.,& Kataranovski, M.. (2016). Strain differences in intestinal toxicity of warfarin in rats. in Environmental Toxicology and Pharmacology
Amsterdam, Holland:Elsevier B.V.., 48, 175-182.
https://doi.org/10.1016/j.etap.2016.10.019

Mirkov I, Popov Aleksandrov A, Ninkov M, Mileusnić D, Demenesku J, Zolotarevski L, Subota V, Stefik D, Kataranovski D, Kataranovski M. Strain differences in intestinal toxicity of warfarin in rats. in Environmental Toxicology and Pharmacology. 2016;48:175-182.
doi:10.1016/j.etap.2016.10.019 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Mileusnić, Dina, Demenesku, Jelena, Zolotarevski, Lidija, Subota, Vesna, Stefik, Debora, Kataranovski, Dragan, Kataranovski, Milena, "Strain differences in intestinal toxicity of warfarin in rats" in Environmental Toxicology and Pharmacology, 48 (2016):175-182,
https://doi.org/10.1016/j.etap.2016.10.019 . .

TY  - JOUR
AU  - Subota, Vesna
AU  - Mirkov, Ivana
AU  - Demenesku, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4814
AB  - Occupational/accidental exposure data have showed hemorrhage as a result of transdermal exposure to
warfarin, however, other effects are not known. In the present study, the impact of epicutaneous application of 10 g or 100 g of warfarin (three times, once a day) on peripheral blood polymorphonuclear
(PMN) and mononuclear cells (PBMC) was examined in rats. Both doses resulted in prolongation of prothrombin time and changes in hematologic parameters. Increases in PMN intracellular myeloperoxidase
(MPO) activity were seen at higher warfarin dose and both doses resulted in higher percentages of granular CD11b+ cells. In contrast, a decrease in PMN TNF and IL-6 production (ELISA) and gene expression
(RT-PCR) was observed. Epicutaneous application of warfarin resulted in decreased numbers of PBMC,
higher numbers of mononuclear CD11b+ cells, but without effect on PMBC cytokine production. The data
obtained showed differential effects of transdermal exposure to warfarin depending on leukocyte type
and activity.
PB  - Amsterdam : Elsevier
T2  - Environmental Toxicology and Pharmacology
T1  - Transdermal toxicity of topically applied anticoagulant rodenticide warfarin in rats
VL  - 41
DO  - 10.1016/j.etap.2015.12.006
SP  - 232
EP  - 240
ER  - 

@article{
author = "Subota, Vesna and Mirkov, Ivana and Demenesku, Jelena and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Mileusnić, Dina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Occupational/accidental exposure data have showed hemorrhage as a result of transdermal exposure to
warfarin, however, other effects are not known. In the present study, the impact of epicutaneous application of 10 g or 100 g of warfarin (three times, once a day) on peripheral blood polymorphonuclear
(PMN) and mononuclear cells (PBMC) was examined in rats. Both doses resulted in prolongation of prothrombin time and changes in hematologic parameters. Increases in PMN intracellular myeloperoxidase
(MPO) activity were seen at higher warfarin dose and both doses resulted in higher percentages of granular CD11b+ cells. In contrast, a decrease in PMN TNF and IL-6 production (ELISA) and gene expression
(RT-PCR) was observed. Epicutaneous application of warfarin resulted in decreased numbers of PBMC,
higher numbers of mononuclear CD11b+ cells, but without effect on PMBC cytokine production. The data
obtained showed differential effects of transdermal exposure to warfarin depending on leukocyte type
and activity.",
publisher = "Amsterdam : Elsevier",
journal = "Environmental Toxicology and Pharmacology",
title = "Transdermal toxicity of topically applied anticoagulant rodenticide warfarin in rats",
volume = "41",
doi = "10.1016/j.etap.2015.12.006",
pages = "232-240"
}

Subota, V., Mirkov, I., Demenesku, J., Popov Aleksandrov, A., Ninkov, M., Mileusnić, D., Kataranovski, D.,& Kataranovski, M.. (2016). Transdermal toxicity of topically applied anticoagulant rodenticide warfarin in rats. in Environmental Toxicology and Pharmacology
Amsterdam : Elsevier., 41, 232-240.
https://doi.org/10.1016/j.etap.2015.12.006

Subota V, Mirkov I, Demenesku J, Popov Aleksandrov A, Ninkov M, Mileusnić D, Kataranovski D, Kataranovski M. Transdermal toxicity of topically applied anticoagulant rodenticide warfarin in rats. in Environmental Toxicology and Pharmacology. 2016;41:232-240.
doi:10.1016/j.etap.2015.12.006 .

Subota, Vesna, Mirkov, Ivana, Demenesku, Jelena, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Mileusnić, Dina, Kataranovski, Dragan, Kataranovski, Milena, "Transdermal toxicity of topically applied anticoagulant rodenticide warfarin in rats" in Environmental Toxicology and Pharmacology, 41 (2016):232-240,
https://doi.org/10.1016/j.etap.2015.12.006 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4815
AB  - Though warfarin is extensively used in the prevention and treatment of thromboembolic
processes in humans, adverse effects of warfarin therapy have been recognized. Intestinal
hemorrhage is one of the hazards of anticoagulant therapy, but the mechanisms of warfarin
toxicity are virtually unknown. In this work, the effects of 30 days oral warfarin (0.35 mg/l and
3.5 mg/l) intake on rat’s gut were examined. Both doses resulted in prolongation of prothrombin
time. Systemic effects of higher warfarin dose (increases in plasma AST, proteinuria, hematuria,
changes in peripheral blood hematological parameters) were seen. Warfarin intake resulted in
histologically evident tissue damage, leukocyte infiltration and intestinal inflammation [increases
in myeloperoxidase activity, malondialdehyde content, superoxide dismutase and catalase
activity, proinflammatory cytokine (IFN-γ, IL-17) concentrations in intestinal homogenates]. In
contrast, suppression of gut-draining mesenteric lymph node (MLN) cell activity [proliferation
responsiveness, production of IFN-γ and IL-17 to T lymphocyte mitogen Concanavalin A
stimulation] was noted. Inhibition of regulatory cytokine IL-10 production by MLN cells,
suggests commitment of MLN to the suppression of all inflammatory activities and creation of
the microenvironment which is non-permissive for induction of potentially harmful immune
response. These novel findings indicate the need of staying alert for (adverse) effects of warfarin
therapy.
PB  - Amsterdam : Elsevier
T2  - Food and Chemical Toxicology
T1  - Intestinal toxicity of oral warfarin intake in rats
VL  - 94
DO  - 10.1016/j.fct.2016.05.007
SP  - 11
EP  - 18
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Ninkov, Marina and Mileusnić, Dina and Zolotarevski, Lidija and Subota, Vesna and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Though warfarin is extensively used in the prevention and treatment of thromboembolic
processes in humans, adverse effects of warfarin therapy have been recognized. Intestinal
hemorrhage is one of the hazards of anticoagulant therapy, but the mechanisms of warfarin
toxicity are virtually unknown. In this work, the effects of 30 days oral warfarin (0.35 mg/l and
3.5 mg/l) intake on rat’s gut were examined. Both doses resulted in prolongation of prothrombin
time. Systemic effects of higher warfarin dose (increases in plasma AST, proteinuria, hematuria,
changes in peripheral blood hematological parameters) were seen. Warfarin intake resulted in
histologically evident tissue damage, leukocyte infiltration and intestinal inflammation [increases
in myeloperoxidase activity, malondialdehyde content, superoxide dismutase and catalase
activity, proinflammatory cytokine (IFN-γ, IL-17) concentrations in intestinal homogenates]. In
contrast, suppression of gut-draining mesenteric lymph node (MLN) cell activity [proliferation
responsiveness, production of IFN-γ and IL-17 to T lymphocyte mitogen Concanavalin A
stimulation] was noted. Inhibition of regulatory cytokine IL-10 production by MLN cells,
suggests commitment of MLN to the suppression of all inflammatory activities and creation of
the microenvironment which is non-permissive for induction of potentially harmful immune
response. These novel findings indicate the need of staying alert for (adverse) effects of warfarin
therapy.",
publisher = "Amsterdam : Elsevier",
journal = "Food and Chemical Toxicology",
title = "Intestinal toxicity of oral warfarin intake in rats",
volume = "94",
doi = "10.1016/j.fct.2016.05.007",
pages = "11-18"
}

Mirkov, I., Popov Aleksandrov, A., Demenesku, J., Ninkov, M., Mileusnić, D., Zolotarevski, L., Subota, V., Kataranovski, D.,& Kataranovski, M.. (2016). Intestinal toxicity of oral warfarin intake in rats. in Food and Chemical Toxicology
Amsterdam : Elsevier., 94, 11-18.
https://doi.org/10.1016/j.fct.2016.05.007

Mirkov I, Popov Aleksandrov A, Demenesku J, Ninkov M, Mileusnić D, Zolotarevski L, Subota V, Kataranovski D, Kataranovski M. Intestinal toxicity of oral warfarin intake in rats. in Food and Chemical Toxicology. 2016;94:11-18.
doi:10.1016/j.fct.2016.05.007 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Ninkov, Marina, Mileusnić, Dina, Zolotarevski, Lidija, Subota, Vesna, Kataranovski, Dragan, Kataranovski, Milena, "Intestinal toxicity of oral warfarin intake in rats" in Food and Chemical Toxicology, 94 (2016):11-18,
https://doi.org/10.1016/j.fct.2016.05.007 . .

TY  - CONF
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Demenesku, Jelena
AU  - Brceski, Ilija
AU  - Tolinacki, Maja
AU  - Jovanovic, Sofija
AU  - Mileusnić, Dina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4854
AB  - Our previous research showed a higher reactivity of DA (compared to AO rats) to antigens that cause skin and lung inflammatory reactions. In order to examine the effect of strains on the immune responses of other regions, the effect of oral (in drinking water, 30 days) intake of cadmium, a known food and water contaminant, on the intestinal immune response of AO and DA rats was analyzed. Despite similar amounts of cadmium deposited in the intestines of both strains, the reduction in Lactobacillus (important for maintaining immune homeostasis in the intestine) and tissue damage (histologically and according to the marker of tissue necrosis, HMGB-1) was more pronounced in DA rats.Changes, including the activity of antioxidant defense enzymes (superoxide dismutase and catalase), increased concentrations of IFN-γ and IL-17, and no changes in IL-10, which were detected in intestinal homogenates only in DA strains, indicate a more intense intestinal inflammatory reaction. compared to AO strain. The same concentrations of cadmium detected in the main draining (mesenteric) lymph nodes (MLC) led to the induction of mRNA for metal-binding redox proteins (metallothioneins, MT) only in DA rats. The presence of a proinflammatory cytokine response (protein products and mRNA) of MLC cells, detected predominantly in DA strains, indicates a more pronounced induction of cells that produce these cytokines.Increased cell proliferation and oxidative activity of MLC cells, as well as the number of CD68 +, NKG2D + and CD11b + cells only in DA rats, with a differential change in IL-10 (decrease in DA, increase in AO) emphasizes the inflammatory character of MLC rat microenvironment of this strain. The absence of similar changes in the spleen (at the same tissue load of cadmium as MLC) indicates the influence of damaged intestinal tissue on the activity of regional lymph nodes, and the more intense response of MLC DA soy reflects greater efforts to prevent systemic immune response to changes in intestinal homeostasis.
PB  - Belgrade: Immunological Society of Serbia
C3  - VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata
T1  - Differential strain impact on immune reactivity: insights from regional immune responses in rats
T1  - Diferencijalni uticaj soja na imunsku reaktivnost: uvid iz regionalnih imunskih odgovora kod pacova
SP  - 10
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4854
ER  - 

@conference{
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Demenesku, Jelena and Brceski, Ilija and Tolinacki, Maja and Jovanovic, Sofija and Mileusnić, Dina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Our previous research showed a higher reactivity of DA (compared to AO rats) to antigens that cause skin and lung inflammatory reactions. In order to examine the effect of strains on the immune responses of other regions, the effect of oral (in drinking water, 30 days) intake of cadmium, a known food and water contaminant, on the intestinal immune response of AO and DA rats was analyzed. Despite similar amounts of cadmium deposited in the intestines of both strains, the reduction in Lactobacillus (important for maintaining immune homeostasis in the intestine) and tissue damage (histologically and according to the marker of tissue necrosis, HMGB-1) was more pronounced in DA rats.Changes, including the activity of antioxidant defense enzymes (superoxide dismutase and catalase), increased concentrations of IFN-γ and IL-17, and no changes in IL-10, which were detected in intestinal homogenates only in DA strains, indicate a more intense intestinal inflammatory reaction. compared to AO strain. The same concentrations of cadmium detected in the main draining (mesenteric) lymph nodes (MLC) led to the induction of mRNA for metal-binding redox proteins (metallothioneins, MT) only in DA rats. The presence of a proinflammatory cytokine response (protein products and mRNA) of MLC cells, detected predominantly in DA strains, indicates a more pronounced induction of cells that produce these cytokines.Increased cell proliferation and oxidative activity of MLC cells, as well as the number of CD68 +, NKG2D + and CD11b + cells only in DA rats, with a differential change in IL-10 (decrease in DA, increase in AO) emphasizes the inflammatory character of MLC rat microenvironment of this strain. The absence of similar changes in the spleen (at the same tissue load of cadmium as MLC) indicates the influence of damaged intestinal tissue on the activity of regional lymph nodes, and the more intense response of MLC DA soy reflects greater efforts to prevent systemic immune response to changes in intestinal homeostasis.",
publisher = "Belgrade: Immunological Society of Serbia",
journal = "VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata",
title = "Differential strain impact on immune reactivity: insights from regional immune responses in rats, Diferencijalni uticaj soja na imunsku reaktivnost: uvid iz regionalnih imunskih odgovora kod pacova",
pages = "10",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4854"
}

Ninkov, M., Popov Aleksandrov, A., Mirkov, I., Demenesku, J., Brceski, I., Tolinacki, M., Jovanovic, S., Mileusnić, D., Kataranovski, D.,& Kataranovski, M.. (2016). Differential strain impact on immune reactivity: insights from regional immune responses in rats. in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata
Belgrade: Immunological Society of Serbia., 10.
https://hdl.handle.net/21.15107/rcub_ibiss_4854

Ninkov M, Popov Aleksandrov A, Mirkov I, Demenesku J, Brceski I, Tolinacki M, Jovanovic S, Mileusnić D, Kataranovski D, Kataranovski M. Differential strain impact on immune reactivity: insights from regional immune responses in rats. in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata. 2016;:10.
https://hdl.handle.net/21.15107/rcub_ibiss_4854 .

Ninkov, Marina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Demenesku, Jelena, Brceski, Ilija, Tolinacki, Maja, Jovanovic, Sofija, Mileusnić, Dina, Kataranovski, Dragan, Kataranovski, Milena, "Differential strain impact on immune reactivity: insights from regional immune responses in rats" in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata (2016):10,
https://hdl.handle.net/21.15107/rcub_ibiss_4854 .

TY  - CONF
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4853
AB  - Warfarin is an anticoagulant that is widely used in the prevention and treatment of thromboembolic disorders in humans. Numerous side effects of oral therapy with this agent are known, which has led to the recommendation for transdermal administration of this agent. This study examined the effect of oral warfarin consumption (0.35 mg / l and 3.5 mg / l in drinking water, 30 days) on the intestinal immune system in rats, as well as the systemic effect (on peripheral blood leukocytes) of epicutaneous administration of this agent. µg or 100 µg, once a day, for three days).The anticoagulant effect, determined on the basis of the increase in prothrombin time, was observed after oral consumption of both doses, as well as after epicutaneous application. Orally administered warfarin leads to histologically evident damage to intestinal tissue and inflammation (cellular infiltration, myeloperoxidase activity, malodialdehyde content and superoxide dismutase and catalase activities), as well as increased concentrations of proinflammatory cytokines (IFN-γ, IL-17) in intestinal homogenate.In mesenteric lymph nodes, however, suppression of the immune response has been observed (decreased ability of cells to proliferate and produce IFN-γ and IL-17 in response to cannavalin A stimulation). Decreased production of IL-10 by mesenteric lymph node cells indicates the formation of a microenvironment that does not allow the activation of a potentially harmful immune response in this tissue. Epicutaneous administration of a higher dose of warfarin leads to an increase in the number of neutrophilic leukocytes and intracellular myeloperoxidase activity, as well as an increase in granular CD11b + cells. In contrast to this increase, a decrease in TNF and IL-6 production as well as mRNA levels for these cytokines was observed.After administration of a higher dose of warfarin, there is a decrease in the number of mononuclear cells with an increase in the presence of CD11b + in this population, but without an effect on cytokine production, indicating the differential effects of transdermal administration of warfarin. Taken together, the results indicate the need to monitor the (adverse) effects of warfarin therapy.
PB  - Belgrade: Immunological Society of Serbia
C3  - VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata
T1  - Immunomodulating effect of oral and transdermal varfarine therapy
T1  - Imunomodulatorni efekti oralne i transdermalne terapije varfarinom
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4853
ER  - 

@conference{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Ninkov, Marina and Mileusnić, Dina and Zolotarevski, Lidija and Subota, Vesna and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Warfarin is an anticoagulant that is widely used in the prevention and treatment of thromboembolic disorders in humans. Numerous side effects of oral therapy with this agent are known, which has led to the recommendation for transdermal administration of this agent. This study examined the effect of oral warfarin consumption (0.35 mg / l and 3.5 mg / l in drinking water, 30 days) on the intestinal immune system in rats, as well as the systemic effect (on peripheral blood leukocytes) of epicutaneous administration of this agent. µg or 100 µg, once a day, for three days).The anticoagulant effect, determined on the basis of the increase in prothrombin time, was observed after oral consumption of both doses, as well as after epicutaneous application. Orally administered warfarin leads to histologically evident damage to intestinal tissue and inflammation (cellular infiltration, myeloperoxidase activity, malodialdehyde content and superoxide dismutase and catalase activities), as well as increased concentrations of proinflammatory cytokines (IFN-γ, IL-17) in intestinal homogenate.In mesenteric lymph nodes, however, suppression of the immune response has been observed (decreased ability of cells to proliferate and produce IFN-γ and IL-17 in response to cannavalin A stimulation). Decreased production of IL-10 by mesenteric lymph node cells indicates the formation of a microenvironment that does not allow the activation of a potentially harmful immune response in this tissue. Epicutaneous administration of a higher dose of warfarin leads to an increase in the number of neutrophilic leukocytes and intracellular myeloperoxidase activity, as well as an increase in granular CD11b + cells. In contrast to this increase, a decrease in TNF and IL-6 production as well as mRNA levels for these cytokines was observed.After administration of a higher dose of warfarin, there is a decrease in the number of mononuclear cells with an increase in the presence of CD11b + in this population, but without an effect on cytokine production, indicating the differential effects of transdermal administration of warfarin. Taken together, the results indicate the need to monitor the (adverse) effects of warfarin therapy.",
publisher = "Belgrade: Immunological Society of Serbia",
journal = "VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata",
title = "Immunomodulating effect of oral and transdermal varfarine therapy, Imunomodulatorni efekti oralne i transdermalne terapije varfarinom",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4853"
}

Mirkov, I., Popov Aleksandrov, A., Demenesku, J., Ninkov, M., Mileusnić, D., Zolotarevski, L., Subota, V., Kataranovski, D.,& Kataranovski, M.. (2016). Immunomodulating effect of oral and transdermal varfarine therapy. in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata
Belgrade: Immunological Society of Serbia., 33.
https://hdl.handle.net/21.15107/rcub_ibiss_4853

Mirkov I, Popov Aleksandrov A, Demenesku J, Ninkov M, Mileusnić D, Zolotarevski L, Subota V, Kataranovski D, Kataranovski M. Immunomodulating effect of oral and transdermal varfarine therapy. in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata. 2016;:33.
https://hdl.handle.net/21.15107/rcub_ibiss_4853 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Ninkov, Marina, Mileusnić, Dina, Zolotarevski, Lidija, Subota, Vesna, Kataranovski, Dragan, Kataranovski, Milena, "Immunomodulating effect of oral and transdermal varfarine therapy" in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata (2016):33,
https://hdl.handle.net/21.15107/rcub_ibiss_4853 .

TY  - JOUR
AU  - Zolotarevski, Lidija
AU  - Jović, Milena
AU  - Popov Aleksandrov, Aleksandra
AU  - Milosavljević, Petar
AU  - Brajušković, Goran
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://www.tandfonline.com/doi/full/10.3109/15569527.2015.1008701
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2971
AB  - CONTEXT: Skin is the target of both acute and chronic exposure to warfarin, coumarin anticoagulant. Single exposure of rat skin to this agent induces early (24 h following epicutaneous administration) local response which might be part of inflammatory/reparatory homeostatic program or introduction to pathological events in exposed skin. OBJECTIVE: To examine time-dependent changes in skin of rats exposed to epicutaneously applied warfarin. MATERIALS AND METHODS: The effect of low (10 μg) and high (100 μg) doses of warfarin on histologically evident changes of epidermis (epidermal thickness) and dermis (numbers of mesenchymal cells and dermal capillaries), skin cell proliferative activity (Ki67(+) and PCNA(+) cells) and apoptotic (TUNEL(+)) and necrotic (ultra structural appearance) cells was examined one, three and seven days after the application. RESULTS: Both warfarin doses affected the majority of skin cell activity, but with differential time-course of skin epidermal and dermal cells state/activity. The occurrence of necrotic/apoptotic epidermal and dermal cells was noted the first day after the application and the activities which point to tissue reparation/remodeling were observed seven days after skin exposure to this agent. DISCUSSION: The observed pattern of changes (early evidence of cell/tissue injury which was later followed by signs of cell activity characteristic for tissue reparation/remodeling) implied warfarin-induced toxicity in skin cells as stimulus for subsequent activities relevant for tissue homeostasis. CONCLUSION: The data presented provide new and additional information concerning skin responses to warfarin that gains access to this tissue.
T2  - Cutaneous and Ocular Toxicology
T2  - Cutaneous and Ocular Toxicology
T1  - Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.
IS  - 1
VL  - 35
DO  - 10.3109/15569527.2015.1008701
SP  - 41
EP  - 48
ER  - 

@article{
author = "Zolotarevski, Lidija and Jović, Milena and Popov Aleksandrov, Aleksandra and Milosavljević, Petar and Brajušković, Goran and Demenesku, Jelena and Mirkov, Ivana and Ninkov, Marina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "CONTEXT: Skin is the target of both acute and chronic exposure to warfarin, coumarin anticoagulant. Single exposure of rat skin to this agent induces early (24 h following epicutaneous administration) local response which might be part of inflammatory/reparatory homeostatic program or introduction to pathological events in exposed skin. OBJECTIVE: To examine time-dependent changes in skin of rats exposed to epicutaneously applied warfarin. MATERIALS AND METHODS: The effect of low (10 μg) and high (100 μg) doses of warfarin on histologically evident changes of epidermis (epidermal thickness) and dermis (numbers of mesenchymal cells and dermal capillaries), skin cell proliferative activity (Ki67(+) and PCNA(+) cells) and apoptotic (TUNEL(+)) and necrotic (ultra structural appearance) cells was examined one, three and seven days after the application. RESULTS: Both warfarin doses affected the majority of skin cell activity, but with differential time-course of skin epidermal and dermal cells state/activity. The occurrence of necrotic/apoptotic epidermal and dermal cells was noted the first day after the application and the activities which point to tissue reparation/remodeling were observed seven days after skin exposure to this agent. DISCUSSION: The observed pattern of changes (early evidence of cell/tissue injury which was later followed by signs of cell activity characteristic for tissue reparation/remodeling) implied warfarin-induced toxicity in skin cells as stimulus for subsequent activities relevant for tissue homeostasis. CONCLUSION: The data presented provide new and additional information concerning skin responses to warfarin that gains access to this tissue.",
journal = "Cutaneous and Ocular Toxicology, Cutaneous and Ocular Toxicology",
title = "Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.",
number = "1",
volume = "35",
doi = "10.3109/15569527.2015.1008701",
pages = "41-48"
}

Zolotarevski, L., Jović, M., Popov Aleksandrov, A., Milosavljević, P., Brajušković, G., Demenesku, J., Mirkov, I., Ninkov, M., Kataranovski, D.,& Kataranovski, M.. (2016). Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.. in Cutaneous and Ocular Toxicology, 35(1), 41-48.
https://doi.org/10.3109/15569527.2015.1008701

Zolotarevski L, Jović M, Popov Aleksandrov A, Milosavljević P, Brajušković G, Demenesku J, Mirkov I, Ninkov M, Kataranovski D, Kataranovski M. Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.. in Cutaneous and Ocular Toxicology. 2016;35(1):41-48.
doi:10.3109/15569527.2015.1008701 .

Zolotarevski, Lidija, Jović, Milena, Popov Aleksandrov, Aleksandra, Milosavljević, Petar, Brajušković, Goran, Demenesku, Jelena, Mirkov, Ivana, Ninkov, Marina, Kataranovski, Dragan, Kataranovski, Milena, "Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity." in Cutaneous and Ocular Toxicology, 35, no. 1 (2016):41-48,
https://doi.org/10.3109/15569527.2015.1008701 . .

TY  - JOUR
AU  - Demenesku, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S037842741630128X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2532
AB  - The impact of genetic background on effects of acute i.p. cadmium administration (0.5 mg/kg and 1 mg/kg) on basic immune activity of spleen and lungs was examined in two rat strains, Albino Oxford (AO) and Dark Agouti (DA), known to react differently to chemicals. More pronounced inhibition of Concanavalin A (ConA)-induced and Interleukin (IL)-2 stimulated spleen cell proliferation as well as higher levels of nitric oxide (known to decrease cell's proliferative ability) in DA rats at 1 mg/kg, along with greater inhibition of ConA-induced Interferon (IFN-γ)-production by total and mononuclear (MNC) spleen cells and IL-17 production by spleen MNC in DA vs. AO rats at this dose show greater susceptibility of this strain to Cd effects on spleen cells response. More pronounced infiltration of neutrophils/CD11b+ cells to lungs of DA rats treated with 1 mg/kg of Cd and decreased IL-17 lung cell responses noted solely in DA rats speaks in favor of their higher susceptibility to this metal. However, lack of strain disparity in lung cells IFN-γ responses show that there are regional differences as well. Novel data from this study depict complexity of the influence of genetic background on the effects of cadmium on host immune reactivity.
T2  - Toxicology Letters
T1  - Strain differences of cadmium-induced toxicity in rats: Insight from spleen and lung immune responses
VL  - 256
DO  - 10.1016/j.toxlet.2016.05.022
SP  - 33
EP  - 43
ER  - 

@article{
author = "Demenesku, Jelena and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Ninkov, Marina and Zolotarevski, Lidija and Kataranovski, Dragan and Brceski, Ilija and Kataranovski, Milena",
year = "2016",
abstract = "The impact of genetic background on effects of acute i.p. cadmium administration (0.5 mg/kg and 1 mg/kg) on basic immune activity of spleen and lungs was examined in two rat strains, Albino Oxford (AO) and Dark Agouti (DA), known to react differently to chemicals. More pronounced inhibition of Concanavalin A (ConA)-induced and Interleukin (IL)-2 stimulated spleen cell proliferation as well as higher levels of nitric oxide (known to decrease cell's proliferative ability) in DA rats at 1 mg/kg, along with greater inhibition of ConA-induced Interferon (IFN-γ)-production by total and mononuclear (MNC) spleen cells and IL-17 production by spleen MNC in DA vs. AO rats at this dose show greater susceptibility of this strain to Cd effects on spleen cells response. More pronounced infiltration of neutrophils/CD11b+ cells to lungs of DA rats treated with 1 mg/kg of Cd and decreased IL-17 lung cell responses noted solely in DA rats speaks in favor of their higher susceptibility to this metal. However, lack of strain disparity in lung cells IFN-γ responses show that there are regional differences as well. Novel data from this study depict complexity of the influence of genetic background on the effects of cadmium on host immune reactivity.",
journal = "Toxicology Letters",
title = "Strain differences of cadmium-induced toxicity in rats: Insight from spleen and lung immune responses",
volume = "256",
doi = "10.1016/j.toxlet.2016.05.022",
pages = "33-43"
}

Demenesku, J., Popov Aleksandrov, A., Mirkov, I., Ninkov, M., Zolotarevski, L., Kataranovski, D., Brceski, I.,& Kataranovski, M.. (2016). Strain differences of cadmium-induced toxicity in rats: Insight from spleen and lung immune responses. in Toxicology Letters, 256, 33-43.
https://doi.org/10.1016/j.toxlet.2016.05.022

Demenesku J, Popov Aleksandrov A, Mirkov I, Ninkov M, Zolotarevski L, Kataranovski D, Brceski I, Kataranovski M. Strain differences of cadmium-induced toxicity in rats: Insight from spleen and lung immune responses. in Toxicology Letters. 2016;256:33-43.
doi:10.1016/j.toxlet.2016.05.022 .

Demenesku, Jelena, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Ninkov, Marina, Zolotarevski, Lidija, Kataranovski, Dragan, Brceski, Ilija, Kataranovski, Milena, "Strain differences of cadmium-induced toxicity in rats: Insight from spleen and lung immune responses" in Toxicology Letters, 256 (2016):33-43,
https://doi.org/10.1016/j.toxlet.2016.05.022 . .

TY  - JOUR
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Demenesku, Jelena
AU  - Tucović, Dina
AU  - Jovanović Stojanov, Sofija
AU  - Golic, Natasa
AU  - Tolinacki, Maja
AU  - Kataranovski, Dragan
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2016
UR  - https://www.sciencedirect.com/science/article/pii/S0278691516302423?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3378
AB  - Influence of genetic background on toxicity of oral cadmium (Cd) administration (30 days, in drinking
water; 5 ppm and 50 ppm of cadmium) was examined in Albino Oxford (AO) and Dark Agouti (DA) rats.
Similar cadmium deposition was noted in gut and draining mesenteric lymph nodes (MLN) of both
strains but intensity and/or the pattern of responses to cadmium in these tissues differ. Less intense
intestinal damage and leukocyte infiltration was observed in gut of cadmium-exposed AO rats. While
gut-associated lymph node cells of DA rats responded to cadmium with an increase of cell proliferation,
oxidative activity, IFN-g, IL-17 production and expression, no changes of these activities of MLN cells of
cadmium-treated AO rats were observed. Spleen, which accumulated cadmium comparable to MLN,
responded to metal by drop in cell viability and by reduced responsiveness of proliferation and cytokine
production to stimulation in DA rats solely, which suggest tissue dependence of cadmium effects. More
pronounced cadmium effects on MLN and spleen cells of DA rats (which accumulated similar cadmium
doses as AO rats), showed greater susceptibility of this strain to cadmium. The results presented, for the
first time, depict the influence of genetic background to effects of oral cadmium administration.
T2  - Food and Chemical Toxicology
T1  - Strain differences in toxicity of oral cadmium intake in rats
VL  - 96
DO  - 10.1016/j.fct.2016.07.021
SP  - 11
EP  - 23
ER  - 

@article{
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Demenesku, Jelena and Tucović, Dina and Jovanović Stojanov, Sofija and Golic, Natasa and Tolinacki, Maja and Kataranovski, Dragan and Brceski, Ilija and Kataranovski, Milena",
year = "2016",
abstract = "Influence of genetic background on toxicity of oral cadmium (Cd) administration (30 days, in drinking
water; 5 ppm and 50 ppm of cadmium) was examined in Albino Oxford (AO) and Dark Agouti (DA) rats.
Similar cadmium deposition was noted in gut and draining mesenteric lymph nodes (MLN) of both
strains but intensity and/or the pattern of responses to cadmium in these tissues differ. Less intense
intestinal damage and leukocyte infiltration was observed in gut of cadmium-exposed AO rats. While
gut-associated lymph node cells of DA rats responded to cadmium with an increase of cell proliferation,
oxidative activity, IFN-g, IL-17 production and expression, no changes of these activities of MLN cells of
cadmium-treated AO rats were observed. Spleen, which accumulated cadmium comparable to MLN,
responded to metal by drop in cell viability and by reduced responsiveness of proliferation and cytokine
production to stimulation in DA rats solely, which suggest tissue dependence of cadmium effects. More
pronounced cadmium effects on MLN and spleen cells of DA rats (which accumulated similar cadmium
doses as AO rats), showed greater susceptibility of this strain to cadmium. The results presented, for the
first time, depict the influence of genetic background to effects of oral cadmium administration.",
journal = "Food and Chemical Toxicology",
title = "Strain differences in toxicity of oral cadmium intake in rats",
volume = "96",
doi = "10.1016/j.fct.2016.07.021",
pages = "11-23"
}

Ninkov, M., Popov Aleksandrov, A., Mirkov, I., Demenesku, J., Tucović, D., Jovanović Stojanov, S., Golic, N., Tolinacki, M., Kataranovski, D., Brceski, I.,& Kataranovski, M.. (2016). Strain differences in toxicity of oral cadmium intake in rats. in Food and Chemical Toxicology, 96, 11-23.
https://doi.org/10.1016/j.fct.2016.07.021

Ninkov M, Popov Aleksandrov A, Mirkov I, Demenesku J, Tucović D, Jovanović Stojanov S, Golic N, Tolinacki M, Kataranovski D, Brceski I, Kataranovski M. Strain differences in toxicity of oral cadmium intake in rats. in Food and Chemical Toxicology. 2016;96:11-23.
doi:10.1016/j.fct.2016.07.021 .

Ninkov, Marina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Demenesku, Jelena, Tucović, Dina, Jovanović Stojanov, Sofija, Golic, Natasa, Tolinacki, Maja, Kataranovski, Dragan, Brceski, Ilija, Kataranovski, Milena, "Strain differences in toxicity of oral cadmium intake in rats" in Food and Chemical Toxicology, 96 (2016):11-23,
https://doi.org/10.1016/j.fct.2016.07.021 . .

TY  - CONF
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Mileusnić, Dina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4808
AB  - Introduction: Conflicting data (suppression, augmentation, no effect) exist concerning cadmium (Cd) effects on immune system depending on activity and tissue examined. This study
investigates responses to acute Cd intoxication in three compartments (peripheral blood, spleen and lungs) in Dark Agouti (DA) and Albino Oxford (AO) rats, which are differently
susceptible to variety of stimuli.
Materials and Methods: Systemic (IL-6, TNF, acute phase proteins) and tissue responses [cell stress (metallothionein/MT gene expression), CD11b expression, and cytokine (IFN-γ,
IL-17, IL-10) production and mRNA expression] were measured following intraperitoneal (1 mg/kg) Cd administration.
Results: Cd induces systemic inflammatory response with similar intensity in both rat strains. Increase in Cd spleen content and MT expression evident in both strains (higher in DA
compared to AO rats) was followed by increase in neutrophil infiltration and CD11b expression (with same intensity). Although in both strains Cd caused decreased IFN-γ, unchanged
IL-17 and lower IL-10 responsiveness (compared to respective control), decrease of IFN-γ was more intense in DA compared to AO rats. In lungs of both strains increased Cd deposition
and MT expression (higher in AO) as well as neutrophil infiltration and CD11b expression (greater in DA) was observed. While decreased IFN-γ was noted in both strains, lower IL-17
and IL-10 (vs. controls) were evident in DA rats solely.
Conclusions: Acute Cd intoxication exerts strain-related effects (both inflammatory and immunosuppressive) depending on tissue and activity investigated, but the effects are more
pronounced in DA rats.
PB  - European Federation of Immunological Societies
C3  - 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 353.
T1  - Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue
SP  - 353
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4808
ER  - 

@conference{
author = "Demenesku, Jelena and Mirkov, Ivana and Ninkov, Marina and Popov Aleksandrov, Aleksandra and Zolotarevski, Lidija and Subota, Vesna and Mileusnić, Dina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2015",
abstract = "Introduction: Conflicting data (suppression, augmentation, no effect) exist concerning cadmium (Cd) effects on immune system depending on activity and tissue examined. This study
investigates responses to acute Cd intoxication in three compartments (peripheral blood, spleen and lungs) in Dark Agouti (DA) and Albino Oxford (AO) rats, which are differently
susceptible to variety of stimuli.
Materials and Methods: Systemic (IL-6, TNF, acute phase proteins) and tissue responses [cell stress (metallothionein/MT gene expression), CD11b expression, and cytokine (IFN-γ,
IL-17, IL-10) production and mRNA expression] were measured following intraperitoneal (1 mg/kg) Cd administration.
Results: Cd induces systemic inflammatory response with similar intensity in both rat strains. Increase in Cd spleen content and MT expression evident in both strains (higher in DA
compared to AO rats) was followed by increase in neutrophil infiltration and CD11b expression (with same intensity). Although in both strains Cd caused decreased IFN-γ, unchanged
IL-17 and lower IL-10 responsiveness (compared to respective control), decrease of IFN-γ was more intense in DA compared to AO rats. In lungs of both strains increased Cd deposition
and MT expression (higher in AO) as well as neutrophil infiltration and CD11b expression (greater in DA) was observed. While decreased IFN-γ was noted in both strains, lower IL-17
and IL-10 (vs. controls) were evident in DA rats solely.
Conclusions: Acute Cd intoxication exerts strain-related effects (both inflammatory and immunosuppressive) depending on tissue and activity investigated, but the effects are more
pronounced in DA rats.",
publisher = "European Federation of Immunological Societies",
journal = "4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 353.",
title = "Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue",
pages = "353",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4808"
}

Demenesku, J., Mirkov, I., Ninkov, M., Popov Aleksandrov, A., Zolotarevski, L., Subota, V., Mileusnić, D., Kataranovski, D.,& Kataranovski, M.. (2015). Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 353.
European Federation of Immunological Societies., 353.
https://hdl.handle.net/21.15107/rcub_ibiss_4808

Demenesku J, Mirkov I, Ninkov M, Popov Aleksandrov A, Zolotarevski L, Subota V, Mileusnić D, Kataranovski D, Kataranovski M. Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 353.. 2015;:353.
https://hdl.handle.net/21.15107/rcub_ibiss_4808 .

Demenesku, Jelena, Mirkov, Ivana, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Zolotarevski, Lidija, Subota, Vesna, Mileusnić, Dina, Kataranovski, Dragan, Kataranovski, Milena, "Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue" in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 353. (2015):353,
https://hdl.handle.net/21.15107/rcub_ibiss_4808 .

TY  - CONF
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Mileusnić, Dina
AU  - Tolinacki, Maja
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4809
AB  - Introduction: Toxic metal cadmium (Cd) is important food and drinking water contaminant. The majority of ingested cadmium retained in the gastrointestinal tract (GIT) mucosa
which allocates GIT as its main target. Mechanisms of induction of intestinal inflammatory response are largely unknown.
Materials and Methods: Effect of subchronic (30 days) oral (in water) cadmium administration (5 ppm and 50 ppm) was examined in Dark Agouti (DA) and Albino Oxford (AO) rats.
Beside intestinal immune response, activity of draining mesenteric lymph node (MLN) cells, central place for induction of intestinal immune tolerance and local protective responses,
was evaluated.
Results: In both rat strains cadmium consumption resulted in reduction of bacteria (Lactobacillus strain), intestinal tissue damage, modulated antioxidant enzymes activity and
inflammation [increased proinflammatory cytokine (TNF, IL-1β, IFN-γ, IL-17) content in DA rats; increased TNF and IL-10 content in AO rats] in duodenal homogenates. Accumulation
of cadmium in MLN was followed by stress response [elevation of MLN glutathione and metallothionein mRNA levels] only in DA rats. Stimulation of both adaptive (proliferation, Th1
and Th17 cytokine response) and innate immune activities (NKG2D+, CD68+ cells, selected oxidative activities, IL-1β) in MLN was observed, more pronounced in DA compared to AO
rats.
Conclusions: Oral intake of cadmium resulted in intestinal damage, inflammation, and induction of proinflammatory milleu and innate effector cell activities in MLN. Cadmiuminduced proinflammatory responses in DA rats but discrete immune responses of AO rats imply strain-dependent effects of oral cadmium administration.
PB  - European Federation of Immunological Societies
C3  - 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.
T1  - Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes
SP  - 486
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4809
ER  - 

@conference{
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Mirkov, Ivana and Mileusnić, Dina and Tolinacki, Maja and Zolotarevski, Lidija and Kataranovski, Dragan and Brceski, Ilija and Kataranovski, Milena",
year = "2015",
abstract = "Introduction: Toxic metal cadmium (Cd) is important food and drinking water contaminant. The majority of ingested cadmium retained in the gastrointestinal tract (GIT) mucosa
which allocates GIT as its main target. Mechanisms of induction of intestinal inflammatory response are largely unknown.
Materials and Methods: Effect of subchronic (30 days) oral (in water) cadmium administration (5 ppm and 50 ppm) was examined in Dark Agouti (DA) and Albino Oxford (AO) rats.
Beside intestinal immune response, activity of draining mesenteric lymph node (MLN) cells, central place for induction of intestinal immune tolerance and local protective responses,
was evaluated.
Results: In both rat strains cadmium consumption resulted in reduction of bacteria (Lactobacillus strain), intestinal tissue damage, modulated antioxidant enzymes activity and
inflammation [increased proinflammatory cytokine (TNF, IL-1β, IFN-γ, IL-17) content in DA rats; increased TNF and IL-10 content in AO rats] in duodenal homogenates. Accumulation
of cadmium in MLN was followed by stress response [elevation of MLN glutathione and metallothionein mRNA levels] only in DA rats. Stimulation of both adaptive (proliferation, Th1
and Th17 cytokine response) and innate immune activities (NKG2D+, CD68+ cells, selected oxidative activities, IL-1β) in MLN was observed, more pronounced in DA compared to AO
rats.
Conclusions: Oral intake of cadmium resulted in intestinal damage, inflammation, and induction of proinflammatory milleu and innate effector cell activities in MLN. Cadmiuminduced proinflammatory responses in DA rats but discrete immune responses of AO rats imply strain-dependent effects of oral cadmium administration.",
publisher = "European Federation of Immunological Societies",
journal = "4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.",
title = "Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes",
pages = "486",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4809"
}

Ninkov, M., Popov Aleksandrov, A., Demenesku, J., Mirkov, I., Mileusnić, D., Tolinacki, M., Zolotarevski, L., Kataranovski, D., Brceski, I.,& Kataranovski, M.. (2015). Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.
European Federation of Immunological Societies., 486.
https://hdl.handle.net/21.15107/rcub_ibiss_4809

Ninkov M, Popov Aleksandrov A, Demenesku J, Mirkov I, Mileusnić D, Tolinacki M, Zolotarevski L, Kataranovski D, Brceski I, Kataranovski M. Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.. 2015;:486.
https://hdl.handle.net/21.15107/rcub_ibiss_4809 .

Ninkov, Marina, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Mirkov, Ivana, Mileusnić, Dina, Tolinacki, Maja, Zolotarevski, Lidija, Kataranovski, Dragan, Brceski, Ilija, Kataranovski, Milena, "Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes" in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486. (2015):486,
https://hdl.handle.net/21.15107/rcub_ibiss_4809 .

TY  - CONF
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Mileusnić, Dina
AU  - Zolotarevski, Lidija
AU  - Tolinački, Maja
AU  - Kataranovski, Dragan
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4810
AB  - Conflicting data exist concerning cadmium
effects on immune system depending on the
experimental model, exposure or tissue/activity
examined. In this study immunotoxicity of acute
intraperitoneal and oral cadmium
administration was investigated in rats. The
use of the inflammation-prone inbred Dark
Agouti (DA) and less reactive Albino Oxford
(AO) rats showed differential (immune activityrelated and/or strain-related) effects of
cadmium (1 mg of Cd/kg, i.p.) on spleen
immune responses. A decrease in ConAinduced proliferation (related to altered spleen
cells responsiveness to IL-2) and of IFN-γ
(independently of IL-4 and IL-10) was more
pronounced in DA rats. Increased innate
immunity splenocyte activity (granulocyte
CD11b+ cells, iNOS mRNA and NO
production, myeloperoxidase MPO activity, IL1β mRNA and IL-1β protein product levels)
were observed in both strains (some of them
more pronounced in DA rats), while a decrease
in respiratory burst (dihydrorhodamine/DHR
oxidation) was similar. 30-day oral intake of 5
ppm and 50 ppm of cadmium by DA rats
resulted in reduction of some probiotic bacteria,
villous damage and intestinal inflammation
[(increased levels of High Mobility Group
Box1/HMGB1, antioxidant enzyme (superoxide
dismutase/SOD and catalase/CAT) activity and
proinflammatory cytokine (TNF, IL-1β, IFN-γ,
IL-17) in gut homogenates]. Stimulation of both
adaptive (increased cellularity, proliferation,
IFN-γ and IL-17cytokine responses) as well as
innate immune activity (increases in numbers
of NK cells and M1-like macrophages,
oxidative cell activities, IL-1β) of gut draining
(mesenteric) lymph nodes was associated with
decreased or unchanged antiinflammatory
cytokine (IL-10) cell response. Differential
(immunosuppressive and immunostimulatory)
effects noted in the same tissue (spleen)
should be taken into account when exploring
immunotoxicity of this metal. Stimulation of gut
immune responses imply dietary cadmium as
health risk factor.
PB  - Department of Biomedical Sciences-Histology of the University of Sassari
C3  - II Cadmium Symposium, June 25-27, 2015, Sassari, Italy
T1  - Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4810
ER  - 

@conference{
author = "Demenesku, Jelena and Ninkov, Marina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Mileusnić, Dina and Zolotarevski, Lidija and Tolinački, Maja and Kataranovski, Dragan and Brceski, Ilija and Kataranovski, Milena",
year = "2015",
abstract = "Conflicting data exist concerning cadmium
effects on immune system depending on the
experimental model, exposure or tissue/activity
examined. In this study immunotoxicity of acute
intraperitoneal and oral cadmium
administration was investigated in rats. The
use of the inflammation-prone inbred Dark
Agouti (DA) and less reactive Albino Oxford
(AO) rats showed differential (immune activityrelated and/or strain-related) effects of
cadmium (1 mg of Cd/kg, i.p.) on spleen
immune responses. A decrease in ConAinduced proliferation (related to altered spleen
cells responsiveness to IL-2) and of IFN-γ
(independently of IL-4 and IL-10) was more
pronounced in DA rats. Increased innate
immunity splenocyte activity (granulocyte
CD11b+ cells, iNOS mRNA and NO
production, myeloperoxidase MPO activity, IL1β mRNA and IL-1β protein product levels)
were observed in both strains (some of them
more pronounced in DA rats), while a decrease
in respiratory burst (dihydrorhodamine/DHR
oxidation) was similar. 30-day oral intake of 5
ppm and 50 ppm of cadmium by DA rats
resulted in reduction of some probiotic bacteria,
villous damage and intestinal inflammation
[(increased levels of High Mobility Group
Box1/HMGB1, antioxidant enzyme (superoxide
dismutase/SOD and catalase/CAT) activity and
proinflammatory cytokine (TNF, IL-1β, IFN-γ,
IL-17) in gut homogenates]. Stimulation of both
adaptive (increased cellularity, proliferation,
IFN-γ and IL-17cytokine responses) as well as
innate immune activity (increases in numbers
of NK cells and M1-like macrophages,
oxidative cell activities, IL-1β) of gut draining
(mesenteric) lymph nodes was associated with
decreased or unchanged antiinflammatory
cytokine (IL-10) cell response. Differential
(immunosuppressive and immunostimulatory)
effects noted in the same tissue (spleen)
should be taken into account when exploring
immunotoxicity of this metal. Stimulation of gut
immune responses imply dietary cadmium as
health risk factor.",
publisher = "Department of Biomedical Sciences-Histology of the University of Sassari",
journal = "II Cadmium Symposium, June 25-27, 2015, Sassari, Italy",
title = "Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats",
pages = "31",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4810"
}

Demenesku, J., Ninkov, M., Popov Aleksandrov, A., Mirkov, I., Mileusnić, D., Zolotarevski, L., Tolinački, M., Kataranovski, D., Brceski, I.,& Kataranovski, M.. (2015). Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats. in II Cadmium Symposium, June 25-27, 2015, Sassari, Italy
Department of Biomedical Sciences-Histology of the University of Sassari., 31.
https://hdl.handle.net/21.15107/rcub_ibiss_4810

Demenesku J, Ninkov M, Popov Aleksandrov A, Mirkov I, Mileusnić D, Zolotarevski L, Tolinački M, Kataranovski D, Brceski I, Kataranovski M. Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats. in II Cadmium Symposium, June 25-27, 2015, Sassari, Italy. 2015;:31.
https://hdl.handle.net/21.15107/rcub_ibiss_4810 .

Demenesku, Jelena, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Mileusnić, Dina, Zolotarevski, Lidija, Tolinački, Maja, Kataranovski, Dragan, Brceski, Ilija, Kataranovski, Milena, "Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats" in II Cadmium Symposium, June 25-27, 2015, Sassari, Italy (2015):31,
https://hdl.handle.net/21.15107/rcub_ibiss_4810 .

TY  - CONF
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Jovanovic, Jelena
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4807
AB  - Introduction: Contact hypersensitivity (CHS) is animal model of allergic contact dermatitis, common skin inflammatory disease. Although mechanisms of CHS are much studied,
systemic effects in challenge phase are far less known.
Materials and methods: Parameters of adaptive and innate/inflammatory reaction were examined locally (ear skin cells) and systemically [peripheral blood mononuclear cells (PBMC)
and spleen cells] 24 hours after challenge, in two rat strains, Dark Agouti (DA) and Albino Oxford (AO), previously shown to differ in intensity of ear swelling response and draining
lymph node cell activity in CHS.
Results: While production of IL-17 by ear skin cells after challenge was increased in both rat strains (vs. controls), production of IFN-γ and CD8+ cell number was increased only in DA.
Activity relevant for innate immunity (CD11b+ skin cell number and NO production) was significantly increased only in DA. Production of proinflammatory cytokines TNF-α and IL-1β
by these cells was unchanged in both strains. Similarly to ear skin cells, IL-17 production by PBMC was increased in both strains while IFN-γ, and TNF-α, IL-1β and NO, only in DA. In
the spleen, however, only IFN-γ production was increased in both strains, while other parameters were variably affected in DA and AO.
Conclusion: In CHS reaction to dinitrochlorobenzene proinflammatory/effector activity detected locally in skin, coincides with PBMC activity (more pronounced in DA compared to AO
rats). Systemic effect was compartment dependent, given differential activity of spleen cells in two strains.
PB  - European Federation of Immunological Societies
C3  - 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria
T1  - Contact hypersensitivity to dinitrochlorobenzene induces systemic immunomodulatory effects that are strain-dependent
SP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4807
ER  - 

@conference{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Demenesku, Jelena and Ninkov, Marina and Mileusnić, Dina and Jovanovic, Jelena and Kataranovski, Dragan and Kataranovski, Milena",
year = "2015",
abstract = "Introduction: Contact hypersensitivity (CHS) is animal model of allergic contact dermatitis, common skin inflammatory disease. Although mechanisms of CHS are much studied,
systemic effects in challenge phase are far less known.
Materials and methods: Parameters of adaptive and innate/inflammatory reaction were examined locally (ear skin cells) and systemically [peripheral blood mononuclear cells (PBMC)
and spleen cells] 24 hours after challenge, in two rat strains, Dark Agouti (DA) and Albino Oxford (AO), previously shown to differ in intensity of ear swelling response and draining
lymph node cell activity in CHS.
Results: While production of IL-17 by ear skin cells after challenge was increased in both rat strains (vs. controls), production of IFN-γ and CD8+ cell number was increased only in DA.
Activity relevant for innate immunity (CD11b+ skin cell number and NO production) was significantly increased only in DA. Production of proinflammatory cytokines TNF-α and IL-1β
by these cells was unchanged in both strains. Similarly to ear skin cells, IL-17 production by PBMC was increased in both strains while IFN-γ, and TNF-α, IL-1β and NO, only in DA. In
the spleen, however, only IFN-γ production was increased in both strains, while other parameters were variably affected in DA and AO.
Conclusion: In CHS reaction to dinitrochlorobenzene proinflammatory/effector activity detected locally in skin, coincides with PBMC activity (more pronounced in DA compared to AO
rats). Systemic effect was compartment dependent, given differential activity of spleen cells in two strains.",
publisher = "European Federation of Immunological Societies",
journal = "4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria",
title = "Contact hypersensitivity to dinitrochlorobenzene induces systemic immunomodulatory effects that are strain-dependent",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4807"
}

Popov Aleksandrov, A., Mirkov, I., Demenesku, J., Ninkov, M., Mileusnić, D., Jovanovic, J., Kataranovski, D.,& Kataranovski, M.. (2015). Contact hypersensitivity to dinitrochlorobenzene induces systemic immunomodulatory effects that are strain-dependent. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria
European Federation of Immunological Societies., 130.
https://hdl.handle.net/21.15107/rcub_ibiss_4807

Popov Aleksandrov A, Mirkov I, Demenesku J, Ninkov M, Mileusnić D, Jovanovic J, Kataranovski D, Kataranovski M. Contact hypersensitivity to dinitrochlorobenzene induces systemic immunomodulatory effects that are strain-dependent. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria. 2015;:130.
https://hdl.handle.net/21.15107/rcub_ibiss_4807 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Demenesku, Jelena, Ninkov, Marina, Mileusnić, Dina, Jovanovic, Jelena, Kataranovski, Dragan, Kataranovski, Milena, "Contact hypersensitivity to dinitrochlorobenzene induces systemic immunomodulatory effects that are strain-dependent" in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria (2015):130,
https://hdl.handle.net/21.15107/rcub_ibiss_4807 .

TY  - JOUR
AU  - Đokić, Jelena
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Subota, Vesna
AU  - Mihajlović, Luka
AU  - Stojadinović, Marija
AU  - Stanić-Vučinić, Dragana
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/566
AB  - Warfarin (3-(α-acetonylbenzyl)-4-hydroxy coumarin) is a vitamin K (VK) antagonist that inhibits vitamin K-dependent (VKD) processes, such as blood coagulation. It also exerts an influence on some non-VKD-related activities. In this study, the effect of sub-acute (30-day) oral warfarin (2 and 1 mg L-1) intake on hematological parameters was examined in two rat strains, Albino Oxford (AO) and Dark Agouti (DA), that differ in their sensitivity to certain chemicals. Greater susceptibility to the anticoagulant effect of 2 mg L-1 of warfarin was observed in AO rats and was associated with an increase in the relevant hematological parameters in this strain. Although both strains responded to 2 mg L-1 of warfarin with quantitative changes in the peripheral blood leukocytes, differential bone marrow and lung responses were observed. Strain-related differences in the pro-inflammatory activity of peripheral blood granulocytes and in mononuclear cell IFN-γ production were observed. Recognition of differences in quantitative and qualitative effects of oral warfarin on processes other than hemostasis might be of relevance for those humans who are on warfarin therapy.
AB  - Varfarin (3-α-acetonilbenzil)-4–hidroksikumarin) je antagonist vitamina K (VK) koji inhibira procese zavisne od ovog vitamina, uključujući koagulaciju krvi. Osim toga, on ispoljava i aktivnosti koje ne zavise od vitamina K kao što su anti-tumorska i imunomodulatorna aktivnost. U ovom radu je ispitan efekat subakutnog (30 dana) oralnog unosa varfarina na hematološke parametre i aktivnost leukocita periferne krvi kod dva soja pacova Albino Oxford (AO) i Dark Agouti (DA) koji se raz- likuju u osetljivosti na iste hemijske agense. Kod jedinki AO soja zapažena je veća smrtnost nakon konzumiranja doze od 4 mg L–1 kao i veća osetljivost na antikoagulantno dejstvo varfarina pri nižim dozama (2 mg L–1) koje je praćeno povećanjem nekih hematoloških parametara. Iako kod jedinki oba soja dolazi do povećanja broja neutrofilnih leukocita periferne krvi pri dozi od 2 mg L–1, promene u osnovnim proinflamatornim aktivnostima ovih ćelija su zapažene samo kod jedinki DA soja. Promene u broju neutrofilnih leukocita u krvi DA jedinki su praćene povećanjem broja granulocitnih prekursora u koštanoj srži, dok prisustvo neutrofila u plućima AO jedinki ukazuje na razmenu ćelija između periferne krvi i plućnog intravaskularnog pula ćelija. Diferencijalne sojno–zavisne promene u aktivnosti mononuklearnih ćelija periferne krvi su takođe zapažene. Razlike u efektu oralno unetog varfarina mogu da imaju implikacije za osobe na oralnoj varfarinskoj terapiji.
T2  - Journal of the Serbian Chemical Society
T1  - Sojne razlike u toksičnosti antagoniste vitamina K varfarina kod pacova
T1  - Strain differences in the toxicity of the vitamin K antagonist warfarin in rats
IS  - 3
VL  - 78
SP  - 381
EP  - 394
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_566
ER  - 

@article{
author = "Đokić, Jelena and Ninkov, Marina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Subota, Vesna and Mihajlović, Luka and Stojadinović, Marija and Stanić-Vučinić, Dragana and Kataranovski, Dragan and Kataranovski, Milena",
year = "2013, 2013",
abstract = "Warfarin (3-(α-acetonylbenzyl)-4-hydroxy coumarin) is a vitamin K (VK) antagonist that inhibits vitamin K-dependent (VKD) processes, such as blood coagulation. It also exerts an influence on some non-VKD-related activities. In this study, the effect of sub-acute (30-day) oral warfarin (2 and 1 mg L-1) intake on hematological parameters was examined in two rat strains, Albino Oxford (AO) and Dark Agouti (DA), that differ in their sensitivity to certain chemicals. Greater susceptibility to the anticoagulant effect of 2 mg L-1 of warfarin was observed in AO rats and was associated with an increase in the relevant hematological parameters in this strain. Although both strains responded to 2 mg L-1 of warfarin with quantitative changes in the peripheral blood leukocytes, differential bone marrow and lung responses were observed. Strain-related differences in the pro-inflammatory activity of peripheral blood granulocytes and in mononuclear cell IFN-γ production were observed. Recognition of differences in quantitative and qualitative effects of oral warfarin on processes other than hemostasis might be of relevance for those humans who are on warfarin therapy., Varfarin (3-α-acetonilbenzil)-4–hidroksikumarin) je antagonist vitamina K (VK) koji inhibira procese zavisne od ovog vitamina, uključujući koagulaciju krvi. Osim toga, on ispoljava i aktivnosti koje ne zavise od vitamina K kao što su anti-tumorska i imunomodulatorna aktivnost. U ovom radu je ispitan efekat subakutnog (30 dana) oralnog unosa varfarina na hematološke parametre i aktivnost leukocita periferne krvi kod dva soja pacova Albino Oxford (AO) i Dark Agouti (DA) koji se raz- likuju u osetljivosti na iste hemijske agense. Kod jedinki AO soja zapažena je veća smrtnost nakon konzumiranja doze od 4 mg L–1 kao i veća osetljivost na antikoagulantno dejstvo varfarina pri nižim dozama (2 mg L–1) koje je praćeno povećanjem nekih hematoloških parametara. Iako kod jedinki oba soja dolazi do povećanja broja neutrofilnih leukocita periferne krvi pri dozi od 2 mg L–1, promene u osnovnim proinflamatornim aktivnostima ovih ćelija su zapažene samo kod jedinki DA soja. Promene u broju neutrofilnih leukocita u krvi DA jedinki su praćene povećanjem broja granulocitnih prekursora u koštanoj srži, dok prisustvo neutrofila u plućima AO jedinki ukazuje na razmenu ćelija između periferne krvi i plućnog intravaskularnog pula ćelija. Diferencijalne sojno–zavisne promene u aktivnosti mononuklearnih ćelija periferne krvi su takođe zapažene. Razlike u efektu oralno unetog varfarina mogu da imaju implikacije za osobe na oralnoj varfarinskoj terapiji.",
journal = "Journal of the Serbian Chemical Society",
title = "Sojne razlike u toksičnosti antagoniste vitamina K varfarina kod pacova, Strain differences in the toxicity of the vitamin K antagonist warfarin in rats",
number = "3",
volume = "78",
pages = "381-394",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_566"
}

Đokić, J., Ninkov, M., Popov Aleksandrov, A., Mirkov, I., Subota, V., Mihajlović, L., Stojadinović, M., Stanić-Vučinić, D., Kataranovski, D.,& Kataranovski, M.. (2013). Sojne razlike u toksičnosti antagoniste vitamina K varfarina kod pacova. in Journal of the Serbian Chemical Society, 78(3), 381-394.
https://hdl.handle.net/21.15107/rcub_ibiss_566

Đokić J, Ninkov M, Popov Aleksandrov A, Mirkov I, Subota V, Mihajlović L, Stojadinović M, Stanić-Vučinić D, Kataranovski D, Kataranovski M. Sojne razlike u toksičnosti antagoniste vitamina K varfarina kod pacova. in Journal of the Serbian Chemical Society. 2013;78(3):381-394.
https://hdl.handle.net/21.15107/rcub_ibiss_566 .

Đokić, Jelena, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Subota, Vesna, Mihajlović, Luka, Stojadinović, Marija, Stanić-Vučinić, Dragana, Kataranovski, Dragan, Kataranovski, Milena, "Sojne razlike u toksičnosti antagoniste vitamina K varfarina kod pacova" in Journal of the Serbian Chemical Society, 78, no. 3 (2013):381-394,
https://hdl.handle.net/21.15107/rcub_ibiss_566 .

TY  - CONF
AU  - Mirkov, Ivana
AU  - El-Muzghi, Amal Atia Mhfuod
AU  - Popov Aleksandrov, Aleksandra
AU  - Đokić, Jelena
AU  - Miljković, Đorđe
AU  - Glamočlija, Jasmina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4101
PB  - Odbor za imunologiju i alergologiju odeljenja medicinskih nauka SANU
C3  - Svestski dan imunologije 2012
T1  - Imunski mehanizmi u rezistenciji na A. fumigatus
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4101
ER  - 

@conference{
author = "Mirkov, Ivana and El-Muzghi, Amal Atia Mhfuod and Popov Aleksandrov, Aleksandra and Đokić, Jelena and Miljković, Đorđe and Glamočlija, Jasmina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2012",
publisher = "Odbor za imunologiju i alergologiju odeljenja medicinskih nauka SANU",
journal = "Svestski dan imunologije 2012",
title = "Imunski mehanizmi u rezistenciji na A. fumigatus",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4101"
}

Mirkov, I., El-Muzghi, A. A. M., Popov Aleksandrov, A., Đokić, J., Miljković, Đ., Glamočlija, J., Kataranovski, D.,& Kataranovski, M.. (2012). Imunski mehanizmi u rezistenciji na A. fumigatus. in Svestski dan imunologije 2012
Odbor za imunologiju i alergologiju odeljenja medicinskih nauka SANU..
https://hdl.handle.net/21.15107/rcub_ibiss_4101

Mirkov I, El-Muzghi AAM, Popov Aleksandrov A, Đokić J, Miljković Đ, Glamočlija J, Kataranovski D, Kataranovski M. Imunski mehanizmi u rezistenciji na A. fumigatus. in Svestski dan imunologije 2012. 2012;.
https://hdl.handle.net/21.15107/rcub_ibiss_4101 .

Mirkov, Ivana, El-Muzghi, Amal Atia Mhfuod, Popov Aleksandrov, Aleksandra, Đokić, Jelena, Miljković, Đorđe, Glamočlija, Jasmina, Kataranovski, Dragan, Kataranovski, Milena, "Imunski mehanizmi u rezistenciji na A. fumigatus" in Svestski dan imunologije 2012 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_4101 .