TY  - DATA
AU  - Ćuković, Katarina
AU  - Todorović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Bogdanović, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6214
AB  - This dataset is a product of the doctoral dissertation titled "Identification and expression analysis of genes involved in somatic embryogenesis of centaury (Centaurium erythraea Rafn.)" funded by the Ministry of Education, Science and Technological Development, Republic of Serbia (University of Belgrade, Institute for Biological Research "Siniša Stanković"). The dataset includes the transcript sequence of a newly discovered marker for early somatic embryogenesis in centaury, named CeNA1 (ID:TR23240|c0_g1_i1), as well as gene, gene promoter and corresponding CeNA1 protein sequences for seven homologs that have intact ORF.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - CeNA1: the newly discovered gene marker for early somatic embryogenesis in centaury (Centaurium erythraea Rafn.) – transcript, gene, gene promoter and protein sequences
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6214
ER  - 

@misc{
author = "Ćuković, Katarina and Todorović, Slađana and Dragićević, Milan and Simonović, Ana and Bogdanović, Milica",
year = "2023",
abstract = "This dataset is a product of the doctoral dissertation titled "Identification and expression analysis of genes involved in somatic embryogenesis of centaury (Centaurium erythraea Rafn.)" funded by the Ministry of Education, Science and Technological Development, Republic of Serbia (University of Belgrade, Institute for Biological Research "Siniša Stanković"). The dataset includes the transcript sequence of a newly discovered marker for early somatic embryogenesis in centaury, named CeNA1 (ID:TR23240|c0_g1_i1), as well as gene, gene promoter and corresponding CeNA1 protein sequences for seven homologs that have intact ORF.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "CeNA1: the newly discovered gene marker for early somatic embryogenesis in centaury (Centaurium erythraea Rafn.) – transcript, gene, gene promoter and protein sequences",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6214"
}

Ćuković, K., Todorović, S., Dragićević, M., Simonović, A.,& Bogdanović, M.. (2023). CeNA1: the newly discovered gene marker for early somatic embryogenesis in centaury (Centaurium erythraea Rafn.) – transcript, gene, gene promoter and protein sequences. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6214

Ćuković K, Todorović S, Dragićević M, Simonović A, Bogdanović M. CeNA1: the newly discovered gene marker for early somatic embryogenesis in centaury (Centaurium erythraea Rafn.) – transcript, gene, gene promoter and protein sequences. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6214 .

Ćuković, Katarina, Todorović, Slađana, Dragićević, Milan, Simonović, Ana, Bogdanović, Milica, "CeNA1: the newly discovered gene marker for early somatic embryogenesis in centaury (Centaurium erythraea Rafn.) – transcript, gene, gene promoter and protein sequences" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6214 .

TY  - CONF
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Aničić, Neda
AU  - Milutinović, Milica
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Šiler, Branislav
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Matekalo, Dragana
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Dragićević, Milan
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5564
AB  - The aim of this study was to determine qualitative composition of methanol-soluble metabolites in leaves
from in vitro-grown plants of phylogenetically distinct Nepeta species producing iridoids (N. sibirica L., N.
spicata Benth., N. argolica Bory & Chaub., and N. rtanjensis Diklić & Milojević) and iridoid-lacking N. nervosa
Royle ex Benth., adopting UHPLC-LTQ OrbiTrap MS technique. Totally, 52 compounds were identified
based on their monoisotopic masses, MSn fragmentation, and previously reported MS data and they were
clustered into four major groups: phenolic acid derivatives (23 compounds), flavonoids (10 compounds),
iridoids (13 compounds), and 6 compounds belonging to other classes. Both phenolic and iridoid profiles
of the tested species were found to be very diverse regarding both qualitative content of compounds and
their relative representation in extracts. Rosmarinic, 5-O-cafeoylquinic, and caffeic acids were found to be
the dominant phenolics in all analyzed species, especially in N. argolica, N. rtanjensis, and N. nervosa.
Iridoids were present in the form of glucosides and the most abundant compounds from this group were
epideoxyloganic acid, geniposide, and aucubin. Although iridoids were not detected in N. nervosa extracts,
this species was revealed as a rich source of phenolic acids and flavonoids. Conversely, N. spicata was found
to produce iridoids but negligible amounts of phenolic compounds. Extracts of two closely related endemic
species originating from the Balkan Peninsula, N. argolica and N. rtanjensis, were very similar in both
phenolic and iridoid profiles. The UHPLC-LTQ OrbiTrap MS method, in combination with chemometrics
data processing, was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic
determination and chemical differentiation of Nepeta species.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
T1  - Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5564
ER  - 

@conference{
author = "Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Aničić, Neda and Milutinović, Milica and Skorić, Marijana and Božunović, Jelena and Petrović, Luka and Šiler, Branislav and Filipović, Biljana and Banjanac, Tijana and Matekalo, Dragana and Todorović, Miloš and Lukić, Tamara and Dragićević, Milan and Mišić, Danijela",
year = "2022",
abstract = "The aim of this study was to determine qualitative composition of methanol-soluble metabolites in leaves
from in vitro-grown plants of phylogenetically distinct Nepeta species producing iridoids (N. sibirica L., N.
spicata Benth., N. argolica Bory & Chaub., and N. rtanjensis Diklić & Milojević) and iridoid-lacking N. nervosa
Royle ex Benth., adopting UHPLC-LTQ OrbiTrap MS technique. Totally, 52 compounds were identified
based on their monoisotopic masses, MSn fragmentation, and previously reported MS data and they were
clustered into four major groups: phenolic acid derivatives (23 compounds), flavonoids (10 compounds),
iridoids (13 compounds), and 6 compounds belonging to other classes. Both phenolic and iridoid profiles
of the tested species were found to be very diverse regarding both qualitative content of compounds and
their relative representation in extracts. Rosmarinic, 5-O-cafeoylquinic, and caffeic acids were found to be
the dominant phenolics in all analyzed species, especially in N. argolica, N. rtanjensis, and N. nervosa.
Iridoids were present in the form of glucosides and the most abundant compounds from this group were
epideoxyloganic acid, geniposide, and aucubin. Although iridoids were not detected in N. nervosa extracts,
this species was revealed as a rich source of phenolic acids and flavonoids. Conversely, N. spicata was found
to produce iridoids but negligible amounts of phenolic compounds. Extracts of two closely related endemic
species originating from the Balkan Peninsula, N. argolica and N. rtanjensis, were very similar in both
phenolic and iridoid profiles. The UHPLC-LTQ OrbiTrap MS method, in combination with chemometrics
data processing, was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic
determination and chemical differentiation of Nepeta species.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia",
title = "Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5564"
}

Gašić, U., Nestorović Živković, J., Dmitrović, S., Aničić, N., Milutinović, M., Skorić, M., Božunović, J., Petrović, L., Šiler, B., Filipović, B., Banjanac, T., Matekalo, D., Todorović, M., Lukić, T., Dragićević, M.,& Mišić, D.. (2022). Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
Ljubljana: Slovenian Society of Plant Biology., 35.
https://hdl.handle.net/21.15107/rcub_ibiss_5564

Gašić U, Nestorović Živković J, Dmitrović S, Aničić N, Milutinović M, Skorić M, Božunović J, Petrović L, Šiler B, Filipović B, Banjanac T, Matekalo D, Todorović M, Lukić T, Dragićević M, Mišić D. Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. 2022;:35.
https://hdl.handle.net/21.15107/rcub_ibiss_5564 .

Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Aničić, Neda, Milutinović, Milica, Skorić, Marijana, Božunović, Jelena, Petrović, Luka, Šiler, Branislav, Filipović, Biljana, Banjanac, Tijana, Matekalo, Dragana, Todorović, Miloš, Lukić, Tamara, Dragićević, Milan, Mišić, Danijela, "Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry" in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia (2022):35,
https://hdl.handle.net/21.15107/rcub_ibiss_5564 .

TY  - CONF
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Dmitrović, Slavica
AU  - Anđelković, Boban
AU  - Dimitrijević, Milena
AU  - Dragićević, Milan
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6563
AB  - Nepeta L. is the largest genus of the Lamiaceae family, and the exclusive representative of the
subfamily Nepetoidae which produces iridoids. However, the genus Nepeta comprises both taxa
producing iridoids (iridoid aglycones-IAs and/or iridoid glucosides-IGs), and those lacking iridoids.
Combining omics-guided approach and functional genomics in elucidating the genetic background
of the iridoid diversity within the genus Nepeta, we provide novel insights into molecular
mechanisms driving the iridoid biosynthesis, and thus into chemical evolution of iridoids within
the genus Nepeta. Multidisciplinary experimental approach is here explained on the example
of iridoid synthases (IS), functionally characterized by in vitro enzymatic assays using recombinant
proteins heterologously expressed in Escherichia coli. Functional ISs are expressed in both
iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa
L. (NnIS). N. nervosa possesses an iridoid biosynthetic platform, but inactive one, most likely due
to the “switching off” or “silencing” of some gene(s) upstream in the pathway, which suggests that
this species has lost the ability to produce iridoids during the evolution. Furthermore, comparative
iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs)
during the exposure of chemodiverse Nepeta taxa to environmental stresses (e.g. dehydration,
UV−B radiation, pathogens) or elicitors (MeJA), facilitated the explanation of regulatory mechanisms
determining the productivity of plants, as well as of factors responsible for the presence/
absence of iridoids or specific groups of iridoids (IAs and IGs), in these plants.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights
SP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6563
ER  - 

@conference{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Božunović, Jelena and Nestorović Živković, Jasmina and Gašić, Uroš and Milutinović, Milica and Dmitrović, Slavica and Anđelković, Boban and Dimitrijević, Milena and Dragićević, Milan and Mišić, Danijela",
year = "2022",
abstract = "Nepeta L. is the largest genus of the Lamiaceae family, and the exclusive representative of the
subfamily Nepetoidae which produces iridoids. However, the genus Nepeta comprises both taxa
producing iridoids (iridoid aglycones-IAs and/or iridoid glucosides-IGs), and those lacking iridoids.
Combining omics-guided approach and functional genomics in elucidating the genetic background
of the iridoid diversity within the genus Nepeta, we provide novel insights into molecular
mechanisms driving the iridoid biosynthesis, and thus into chemical evolution of iridoids within
the genus Nepeta. Multidisciplinary experimental approach is here explained on the example
of iridoid synthases (IS), functionally characterized by in vitro enzymatic assays using recombinant
proteins heterologously expressed in Escherichia coli. Functional ISs are expressed in both
iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa
L. (NnIS). N. nervosa possesses an iridoid biosynthetic platform, but inactive one, most likely due
to the “switching off” or “silencing” of some gene(s) upstream in the pathway, which suggests that
this species has lost the ability to produce iridoids during the evolution. Furthermore, comparative
iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs)
during the exposure of chemodiverse Nepeta taxa to environmental stresses (e.g. dehydration,
UV−B radiation, pathogens) or elicitors (MeJA), facilitated the explanation of regulatory mechanisms
determining the productivity of plants, as well as of factors responsible for the presence/
absence of iridoids or specific groups of iridoids (IAs and IGs), in these plants.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights",
pages = "136",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6563"
}

Aničić, N., Matekalo, D., Skorić, M., Božunović, J., Nestorović Živković, J., Gašić, U., Milutinović, M., Dmitrović, S., Anđelković, B., Dimitrijević, M., Dragićević, M.,& Mišić, D.. (2022). Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 136.
https://hdl.handle.net/21.15107/rcub_ibiss_6563

Aničić N, Matekalo D, Skorić M, Božunović J, Nestorović Živković J, Gašić U, Milutinović M, Dmitrović S, Anđelković B, Dimitrijević M, Dragićević M, Mišić D. Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:136.
https://hdl.handle.net/21.15107/rcub_ibiss_6563 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Božunović, Jelena, Nestorović Živković, Jasmina, Gašić, Uroš, Milutinović, Milica, Dmitrović, Slavica, Anđelković, Boban, Dimitrijević, Milena, Dragićević, Milan, Mišić, Danijela, "Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):136,
https://hdl.handle.net/21.15107/rcub_ibiss_6563 .

TY  - CONF
AU  - Banjanac, Tijana
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Aničić, Neda
AU  - Milutinović, Milica
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Šiler, Branislav
AU  - Filipović, Biljana
AU  - Matekalo, Dragana
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Dragićević, Milan
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5576
AB  - Nepeta species (fam. Lamiace, subfam. Nepetoideae, tribe Nepetae) are well known for their
pharmacological properties, biopesticide potential and behavioral effects on cats. Different biological activities of Nepeta species are closely related to their secondary metabolites, among which
iridoids predominate. Research objective of this study was to determine profiles of methanol-soluble iridoids in leaves of greenhouse-grown plants of 10 phylodiverse Nepeta species: N. grandiflora
M. Bieb., N. laevigata (D. Don) Hand.-Mazz., N. nervosa Royle ex Benth., N. stewartiana Diels, N. nuda
L., N. parnassica Heldr. & Sart., N. rtanjensis Diklić & Milojević, N. ernesti-mayeri Diklić & V. Nikolić, N.
cataria L., and N. sibirica L. Untargeted metabolomics approach adopting UHPLC/LTQ Orbitrap MSn
analysis in a negative ionization mode revealed the presence of totally 15 iridoids in samples, of
which 13 were glucosides and two were aglycons of iridoids. Iridoid compounds were identified
based on their monoisotopic masses, MSn
 fragmentation, and previously reported MS data. The
results indicated that the iridoid profiles of the investigated species are very diverse both in terms
of qualitative content of the compounds and their relative abundance in extracts. Among analysed Nepeta species, Balkan Peninsula endemics (N. rtanjensis, N. parnassica, and N. ernesti-mayeri)
and N. cataria, are especially rich in isomers of 1,5,9-epideoxyloganic acid. 5-Deoxylamiol, nepetariaside, nepetanudoside B, and ajugol were detected in majority of analyzed species. The UHPLC-LTQ OrbiTrap MS analysis, in combination with chemometric data processing, has proven to
be an efficient tool for chemical differentiation between Nepeta taxa, and could aid the efforts to
unravel the chemical evolution within the genus.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae)
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5576
ER  - 

@conference{
author = "Banjanac, Tijana and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Aničić, Neda and Milutinović, Milica and Skorić, Marijana and Božunović, Jelena and Petrović, Luka and Šiler, Branislav and Filipović, Biljana and Matekalo, Dragana and Todorović, Miloš and Lukić, Tamara and Dragićević, Milan and Mišić, Danijela",
year = "2022",
abstract = "Nepeta species (fam. Lamiace, subfam. Nepetoideae, tribe Nepetae) are well known for their
pharmacological properties, biopesticide potential and behavioral effects on cats. Different biological activities of Nepeta species are closely related to their secondary metabolites, among which
iridoids predominate. Research objective of this study was to determine profiles of methanol-soluble iridoids in leaves of greenhouse-grown plants of 10 phylodiverse Nepeta species: N. grandiflora
M. Bieb., N. laevigata (D. Don) Hand.-Mazz., N. nervosa Royle ex Benth., N. stewartiana Diels, N. nuda
L., N. parnassica Heldr. & Sart., N. rtanjensis Diklić & Milojević, N. ernesti-mayeri Diklić & V. Nikolić, N.
cataria L., and N. sibirica L. Untargeted metabolomics approach adopting UHPLC/LTQ Orbitrap MSn
analysis in a negative ionization mode revealed the presence of totally 15 iridoids in samples, of
which 13 were glucosides and two were aglycons of iridoids. Iridoid compounds were identified
based on their monoisotopic masses, MSn
 fragmentation, and previously reported MS data. The
results indicated that the iridoid profiles of the investigated species are very diverse both in terms
of qualitative content of the compounds and their relative abundance in extracts. Among analysed Nepeta species, Balkan Peninsula endemics (N. rtanjensis, N. parnassica, and N. ernesti-mayeri)
and N. cataria, are especially rich in isomers of 1,5,9-epideoxyloganic acid. 5-Deoxylamiol, nepetariaside, nepetanudoside B, and ajugol were detected in majority of analyzed species. The UHPLC-LTQ OrbiTrap MS analysis, in combination with chemometric data processing, has proven to
be an efficient tool for chemical differentiation between Nepeta taxa, and could aid the efforts to
unravel the chemical evolution within the genus.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae)",
pages = "151",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5576"
}

Banjanac, T., Gašić, U., Nestorović Živković, J., Dmitrović, S., Aničić, N., Milutinović, M., Skorić, M., Božunović, J., Petrović, L., Šiler, B., Filipović, B., Matekalo, D., Todorović, M., Lukić, T., Dragićević, M.,& Mišić, D.. (2022). Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae). in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 151.
https://hdl.handle.net/21.15107/rcub_ibiss_5576

Banjanac T, Gašić U, Nestorović Živković J, Dmitrović S, Aničić N, Milutinović M, Skorić M, Božunović J, Petrović L, Šiler B, Filipović B, Matekalo D, Todorović M, Lukić T, Dragićević M, Mišić D. Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae). in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:151.
https://hdl.handle.net/21.15107/rcub_ibiss_5576 .

Banjanac, Tijana, Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Aničić, Neda, Milutinović, Milica, Skorić, Marijana, Božunović, Jelena, Petrović, Luka, Šiler, Branislav, Filipović, Biljana, Matekalo, Dragana, Todorović, Miloš, Lukić, Tamara, Dragićević, Milan, Mišić, Danijela, "Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae)" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):151,
https://hdl.handle.net/21.15107/rcub_ibiss_5576 .

TY  - GEN
AU  - Filipović, Biljana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Dragićević, Milan
AU  - Božunović, Jelena
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Bohanec, Borut
AU  - Milutinović, Milica
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6326
AB  - Kičica (Centaurium erythraea Rafn) je bogat izvor brojnih bioaktivnih jedinjenja, među kojima su najzastupljeniji sekoiridoidi i ksantoni. Poznato je da na akumulaciju specijalizovanih metabolita u lekovitim biljkama utiču razni faktori (ploidnost i starost biljke, tip biljnog organa, itd.). U cilju ispitivanja optimalne produkcije sekoiridoida i ksantona radi boljeg iskorišćavanja biotehnološkog potencijala kičice praćene su promene biosinteze ovih specijalizovanih metabolita u izdancima i korenovima diploidne i tetraploidne kičice tokom tri meseca in vitro razvića. Rezultati uporedne analize ekspresije gena uključenih u biosintetske puteve sekoiridoida i ksantona i fitohemijskog profilisanja ovih jedinjenja su pokazali da su izdanci glavno mesto njihove akumulacije, kao i da njihov odnos varira tokom razvića. Koordinisana ekspresija biosintetskih gena uključenih u metabolizam iridoida i ksantona je regulisana na nivou transkripcije kako tokom razvića tako i na nivou organa. Povišeni nivoi ekspresije biosintetskih gena, ključnih za regulisanje metaboličkog fluksa, su u korelaciji sa sadržajem iridoida i ksantona. Tako je pojačana ekspresija GES, 8HGO i 7DLH2 praćena povećanom produkcijom iridoida, dok je viši nivo transkripata BS i 3HBL pozitivno korelisan sa akumulacijom heksa-supstituisanih ksantona. Može se zaključiti da je diploidni genotip perspektivniji od tetraploidnog u kontrolisanim in vitro uslovima kako u pogledu produkcije iridoida i ksantona, tako i u pogledu prirasta biomase.
AB  - Кичица (Centaurium erythraea Rafn) је богат извор бројних биоактивних једињења,
међу којима су најзаступљенији секоиридоиди и ксантони.1 Познато је да на
акумулацију специјализованих метаболита у лековитим биљкама утичу разни
фактори (плоидност1 и старост биљке, тип биљног органа, итд.). У циљу
испитивања оптималне продукције секоиридоида и ксантона ради бољег
искоришћавања биотехнолошког потенцијала кичице праћене су промене
биосинтезе ових специјализованих метаболита у изданцима и кореновима
диплоидне и тетраплоидне кичице током три месеца in vitro развића.2 Резултати
упоредне анализе експресије гена укључених у биосинтетске путеве секоиридоида
и ксантона и фитохемијског профилисања ових једињења су показали да су
изданци главно место њихове акумулације, као и да њихов однос варира током
развића. Координисана експресија биосинтетских гена укључених у метаболизам
иридоида и ксантона је регулисана на нивоу транскрипције како током развића
тако и на нивоу органа. Повишени нивои експресије биосинтетских гена, кључних
за регулисање метаболичког флукса, су у корелацији са садржајем иридоида и
ксантона. Тако је појачана експресија GES, 8HGO и 7DLH2 праћена повећаном
продукцијом иридоида, док је виши ниво транскрипата BS и 3HBL позитивно
корелисан са акумулацијом хекса-супституисаних ксантона. Може се закључити да
је диплоидни генотип перспективнији од тетраплоидног у контролисаним in vitro
условима како у погледу продукције иридоида и ксантона, тако и у погледу
прираста биомасе.
PB  - Belgrade: Serbian Biological Society
T2  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Prostorni i vremenski obrazac biosinteze sekoiridoida i ksantona tokom ranog razvića diploidne i tetraploidne kičice
T1  - Просторни и временски образац биосинтезе секоиридоида и ксантона током раног развића диплоидне и тетраплоидне кичице
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6326
ER  - 

@misc{
author = "Filipović, Biljana and Skorić, Marijana and Gašić, Uroš and Dragićević, Milan and Božunović, Jelena and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Šiler, Branislav and Bohanec, Borut and Milutinović, Milica and Mišić, Danijela",
year = "2022",
abstract = "Kičica (Centaurium erythraea Rafn) je bogat izvor brojnih bioaktivnih jedinjenja, među kojima su najzastupljeniji sekoiridoidi i ksantoni. Poznato je da na akumulaciju specijalizovanih metabolita u lekovitim biljkama utiču razni faktori (ploidnost i starost biljke, tip biljnog organa, itd.). U cilju ispitivanja optimalne produkcije sekoiridoida i ksantona radi boljeg iskorišćavanja biotehnološkog potencijala kičice praćene su promene biosinteze ovih specijalizovanih metabolita u izdancima i korenovima diploidne i tetraploidne kičice tokom tri meseca in vitro razvića. Rezultati uporedne analize ekspresije gena uključenih u biosintetske puteve sekoiridoida i ksantona i fitohemijskog profilisanja ovih jedinjenja su pokazali da su izdanci glavno mesto njihove akumulacije, kao i da njihov odnos varira tokom razvića. Koordinisana ekspresija biosintetskih gena uključenih u metabolizam iridoida i ksantona je regulisana na nivou transkripcije kako tokom razvića tako i na nivou organa. Povišeni nivoi ekspresije biosintetskih gena, ključnih za regulisanje metaboličkog fluksa, su u korelaciji sa sadržajem iridoida i ksantona. Tako je pojačana ekspresija GES, 8HGO i 7DLH2 praćena povećanom produkcijom iridoida, dok je viši nivo transkripata BS i 3HBL pozitivno korelisan sa akumulacijom heksa-supstituisanih ksantona. Može se zaključiti da je diploidni genotip perspektivniji od tetraploidnog u kontrolisanim in vitro uslovima kako u pogledu produkcije iridoida i ksantona, tako i u pogledu prirasta biomase., Кичица (Centaurium erythraea Rafn) је богат извор бројних биоактивних једињења,
међу којима су најзаступљенији секоиридоиди и ксантони.1 Познато је да на
акумулацију специјализованих метаболита у лековитим биљкама утичу разни
фактори (плоидност1 и старост биљке, тип биљног органа, итд.). У циљу
испитивања оптималне продукције секоиридоида и ксантона ради бољег
искоришћавања биотехнолошког потенцијала кичице праћене су промене
биосинтезе ових специјализованих метаболита у изданцима и кореновима
диплоидне и тетраплоидне кичице током три месеца in vitro развића.2 Резултати
упоредне анализе експресије гена укључених у биосинтетске путеве секоиридоида
и ксантона и фитохемијског профилисања ових једињења су показали да су
изданци главно место њихове акумулације, као и да њихов однос варира током
развића. Координисана експресија биосинтетских гена укључених у метаболизам
иридоида и ксантона је регулисана на нивоу транскрипције како током развића
тако и на нивоу органа. Повишени нивои експресије биосинтетских гена, кључних
за регулисање метаболичког флукса, су у корелацији са садржајем иридоида и
ксантона. Тако је појачана експресија GES, 8HGO и 7DLH2 праћена повећаном
продукцијом иридоида, док је виши ниво транскрипата BS и 3HBL позитивно
корелисан са акумулацијом хекса-супституисаних ксантона. Може се закључити да
је диплоидни генотип перспективнији од тетраплоидног у контролисаним in vitro
условима како у погледу продукције иридоида и ксантона, тако и у погледу
прираста биомасе.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Prostorni i vremenski obrazac biosinteze sekoiridoida i ksantona tokom ranog razvića diploidne i tetraploidne kičice, Просторни и временски образац биосинтезе секоиридоида и ксантона током раног развића диплоидне и тетраплоидне кичице",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6326"
}

Filipović, B., Skorić, M., Gašić, U., Dragićević, M., Božunović, J., Matekalo, D., Nestorović Živković, J., Banjanac, T., Šiler, B., Bohanec, B., Milutinović, M.,& Mišić, D.. (2022). Prostorni i vremenski obrazac biosinteze sekoiridoida i ksantona tokom ranog razvića diploidne i tetraploidne kičice. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 33.
https://hdl.handle.net/21.15107/rcub_ibiss_6326

Filipović B, Skorić M, Gašić U, Dragićević M, Božunović J, Matekalo D, Nestorović Živković J, Banjanac T, Šiler B, Bohanec B, Milutinović M, Mišić D. Prostorni i vremenski obrazac biosinteze sekoiridoida i ksantona tokom ranog razvića diploidne i tetraploidne kičice. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:33.
https://hdl.handle.net/21.15107/rcub_ibiss_6326 .

Filipović, Biljana, Skorić, Marijana, Gašić, Uroš, Dragićević, Milan, Božunović, Jelena, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Šiler, Branislav, Bohanec, Borut, Milutinović, Milica, Mišić, Danijela, "Prostorni i vremenski obrazac biosinteze sekoiridoida i ksantona tokom ranog razvića diploidne i tetraploidne kičice" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):33,
https://hdl.handle.net/21.15107/rcub_ibiss_6326 .

TY  - JOUR
AU  - Filipović, Biljana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Dragićević, Milan
AU  - Božunović, Jelena
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Bohanec, Borut
AU  - Milutinović, Milica
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5254
AB  - Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.
PB  - Amsterdam : Elsevier
T2  - Industrial Crops and Products
T1  - Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level
VL  - 186
DO  - 10.1016/j.indcrop.2022.115146
SP  - 115146
ER  - 

@article{
author = "Filipović, Biljana and Skorić, Marijana and Gašić, Uroš and Dragićević, Milan and Božunović, Jelena and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Šiler, Branislav and Bohanec, Borut and Milutinović, Milica and Mišić, Danijela",
year = "2022",
abstract = "Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.",
publisher = "Amsterdam : Elsevier",
journal = "Industrial Crops and Products",
title = "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level",
volume = "186",
doi = "10.1016/j.indcrop.2022.115146",
pages = "115146"
}

Filipović, B., Skorić, M., Gašić, U., Dragićević, M., Božunović, J., Matekalo, D., Nestorović Živković, J., Banjanac, T., Šiler, B., Bohanec, B., Milutinović, M.,& Mišić, D.. (2022). Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products
Amsterdam : Elsevier., 186, 115146.
https://doi.org/10.1016/j.indcrop.2022.115146

Filipović B, Skorić M, Gašić U, Dragićević M, Božunović J, Matekalo D, Nestorović Živković J, Banjanac T, Šiler B, Bohanec B, Milutinović M, Mišić D. Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products. 2022;186:115146.
doi:10.1016/j.indcrop.2022.115146 .

Filipović, Biljana, Skorić, Marijana, Gašić, Uroš, Dragićević, Milan, Božunović, Jelena, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Šiler, Branislav, Bohanec, Borut, Milutinović, Milica, Mišić, Danijela, "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level" in Industrial Crops and Products, 186 (2022):115146,
https://doi.org/10.1016/j.indcrop.2022.115146 . .

TY  - JOUR
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Živković, Suzana
AU  - Dragićević, Milan
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Petrović, Luka
AU  - Mišić, Danijela
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2022.914138/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9260424
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5073
AB  - Secoiridoid glucosides (SGs) are monoterpenoids derived from the iridoid cyclopentane-C-pyran skeleton with β-D glucose linked at C1 position. Coordinated metabolic processes, such as biosynthesis and catabolism of SGs, ensure constitutive presence of these bitter tasting compounds in plant tissues, which plays a decisive role in the defense against pathogens and herbivores. These compounds are susceptible to hydrolysis mediated by enzymes β-glucosidases, and the resulting aglycones are subsequently directed toward different metabolic pathways in plants. Function of two β-D-glucosidases (named CeBGlu1 and CeBGlu2) from centaury (Centaurium erythraea Rafn; fam. Gentianaceae), belonging to the glycoside hydrolase 1 (GH1) family, was confirmed using in vitro assays with recombinant proteins, following their heterologous expression in E. coli and His-tag affinity purification. Although they show slightly differential substrate preference, both isoforms display high specificity toward SGs and the organ-specific distribution of transcripts was positively correlated with the content of SGs in diploid and tetraploid C. erythraea plants. Transient overexpression of CeBGlu1 and CeBGlu2 in C. erythraea leaves induced changes in metabolite profiles. The effectiveness of transgene overexpression has been altered by plant ploidy. UHPLC/DAD/(±)HESI - MS2 profiling of leaves of diploid and tetraploid C. erythraea genotypes revealed that the amounts of major SGs; sweroside, swertiamarin, and gentiopicrin was decreased in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. The work demonstrates that in planta metabolic engineering adopting transient overexpression of CeBGlu1 and CeBGlu2 is a suitable tool for the modulation of SGs content and glucosides/aglycones ratio, which might have substantial effects on overall phytochemistry of C. erythraea.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Plant Science
T1  - Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.
VL  - 13
DO  - 10.3389/fpls.2022.914138
SP  - 914138
ER  - 

@article{
author = "Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Skorić, Marijana and Matekalo, Dragana and Živković, Suzana and Dragićević, Milan and Filipović, Biljana and Banjanac, Tijana and Petrović, Luka and Mišić, Danijela",
year = "2022",
abstract = "Secoiridoid glucosides (SGs) are monoterpenoids derived from the iridoid cyclopentane-C-pyran skeleton with β-D glucose linked at C1 position. Coordinated metabolic processes, such as biosynthesis and catabolism of SGs, ensure constitutive presence of these bitter tasting compounds in plant tissues, which plays a decisive role in the defense against pathogens and herbivores. These compounds are susceptible to hydrolysis mediated by enzymes β-glucosidases, and the resulting aglycones are subsequently directed toward different metabolic pathways in plants. Function of two β-D-glucosidases (named CeBGlu1 and CeBGlu2) from centaury (Centaurium erythraea Rafn; fam. Gentianaceae), belonging to the glycoside hydrolase 1 (GH1) family, was confirmed using in vitro assays with recombinant proteins, following their heterologous expression in E. coli and His-tag affinity purification. Although they show slightly differential substrate preference, both isoforms display high specificity toward SGs and the organ-specific distribution of transcripts was positively correlated with the content of SGs in diploid and tetraploid C. erythraea plants. Transient overexpression of CeBGlu1 and CeBGlu2 in C. erythraea leaves induced changes in metabolite profiles. The effectiveness of transgene overexpression has been altered by plant ploidy. UHPLC/DAD/(±)HESI - MS2 profiling of leaves of diploid and tetraploid C. erythraea genotypes revealed that the amounts of major SGs; sweroside, swertiamarin, and gentiopicrin was decreased in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. The work demonstrates that in planta metabolic engineering adopting transient overexpression of CeBGlu1 and CeBGlu2 is a suitable tool for the modulation of SGs content and glucosides/aglycones ratio, which might have substantial effects on overall phytochemistry of C. erythraea.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Plant Science",
title = "Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.",
volume = "13",
doi = "10.3389/fpls.2022.914138",
pages = "914138"
}

Božunović, J., Milutinović, M., Aničić, N., Skorić, M., Matekalo, D., Živković, S., Dragićević, M., Filipović, B., Banjanac, T., Petrović, L.,& Mišić, D.. (2022). Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.. in Frontiers in Plant Science
Lausanne: Frontiers Media S.A.., 13, 914138.
https://doi.org/10.3389/fpls.2022.914138

Božunović J, Milutinović M, Aničić N, Skorić M, Matekalo D, Živković S, Dragićević M, Filipović B, Banjanac T, Petrović L, Mišić D. Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.. in Frontiers in Plant Science. 2022;13:914138.
doi:10.3389/fpls.2022.914138 .

Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Skorić, Marijana, Matekalo, Dragana, Živković, Suzana, Dragićević, Milan, Filipović, Biljana, Banjanac, Tijana, Petrović, Luka, Mišić, Danijela, "Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn." in Frontiers in Plant Science, 13 (2022):914138,
https://doi.org/10.3389/fpls.2022.914138 . .

TY  - JOUR
AU  - Filipović, Biljana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Dragićević, Milan
AU  - Božunović, Jelena
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Bohanec, Borut
AU  - Milutinović, Milica
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5000
AB  - Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.
PB  - Amsterdam : Elsevier
T2  - Industrial Crops and Products
T1  - Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level
VL  - 186
DO  - 10.1016/j.indcrop.2022.115146
SP  - 115146
ER  - 

@article{
author = "Filipović, Biljana and Skorić, Marijana and Gašić, Uroš and Dragićević, Milan and Božunović, Jelena and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Šiler, Branislav and Bohanec, Borut and Milutinović, Milica and Mišić, Danijela",
year = "2022",
abstract = "Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.",
publisher = "Amsterdam : Elsevier",
journal = "Industrial Crops and Products",
title = "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level",
volume = "186",
doi = "10.1016/j.indcrop.2022.115146",
pages = "115146"
}

Filipović, B., Skorić, M., Gašić, U., Dragićević, M., Božunović, J., Matekalo, D., Nestorović Živković, J., Banjanac, T., Šiler, B., Bohanec, B., Milutinović, M.,& Mišić, D.. (2022). Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products
Amsterdam : Elsevier., 186, 115146.
https://doi.org/10.1016/j.indcrop.2022.115146

Filipović B, Skorić M, Gašić U, Dragićević M, Božunović J, Matekalo D, Nestorović Živković J, Banjanac T, Šiler B, Bohanec B, Milutinović M, Mišić D. Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products. 2022;186:115146.
doi:10.1016/j.indcrop.2022.115146 .

Filipović, Biljana, Skorić, Marijana, Gašić, Uroš, Dragićević, Milan, Božunović, Jelena, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Šiler, Branislav, Bohanec, Borut, Milutinović, Milica, Mišić, Danijela, "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level" in Industrial Crops and Products, 186 (2022):115146,
https://doi.org/10.1016/j.indcrop.2022.115146 . .

TY  - JOUR
AU  - Rudić, Jelena
AU  - Dragićević, Milan
AU  - Momčilović, Ivana
AU  - Simonović, Ana
AU  - Pantelić, Danijel
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/3/488
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4875
AB  - Potato (Solanum tuberosum L.) is the most important vegetable crop globally and is very susceptible to high ambient temperatures. Since heat stress causes the accumulation of reactive oxygen species (ROS), investigations regarding major enzymatic components of the antioxidative system are of the essence. Superoxide dismutases (SODs) represent the first line of defense against ROS but detailed in silico analysis and characterization of the potato SOD gene family have not been performed thus far. We have analyzed eight functional SOD genes, three StCuZnSODs, one StMnSOD, and four StFeSODs, annotated in the updated version of potato genome (Spud DB DM v6.1). The StSOD genes and their respective proteins were analyzed in silico to determine the exon-intron organization, splice variants, cis-regulatory promoter elements, conserved domains, signals for subcellular targeting, 3D-structures, and phylogenetic relations. Quantitative PCR analysis revealed higher induction of StCuZnSODs (the major potato SODs) and StFeSOD3 in thermotolerant cultivar Désirée than in thermosensitive Agria and Kennebec during long-term exposure to elevated temperature. StMnSOD was constitutively expressed, while expression of StFeSODs was cultivar-dependent. The effects of salicylic acid (10−5 M) on StSODs expression were minor. Our results provide the basis for further research on StSODs and their regulation in potato, particularly in response to elevated temperatures.
T2  - Antioxidants
T1  - In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression
IS  - 3
VL  - 11
DO  - 10.3390/antiox11030488
SP  - 488
ER  - 

@article{
author = "Rudić, Jelena and Dragićević, Milan and Momčilović, Ivana and Simonović, Ana and Pantelić, Danijel",
year = "2022",
abstract = "Potato (Solanum tuberosum L.) is the most important vegetable crop globally and is very susceptible to high ambient temperatures. Since heat stress causes the accumulation of reactive oxygen species (ROS), investigations regarding major enzymatic components of the antioxidative system are of the essence. Superoxide dismutases (SODs) represent the first line of defense against ROS but detailed in silico analysis and characterization of the potato SOD gene family have not been performed thus far. We have analyzed eight functional SOD genes, three StCuZnSODs, one StMnSOD, and four StFeSODs, annotated in the updated version of potato genome (Spud DB DM v6.1). The StSOD genes and their respective proteins were analyzed in silico to determine the exon-intron organization, splice variants, cis-regulatory promoter elements, conserved domains, signals for subcellular targeting, 3D-structures, and phylogenetic relations. Quantitative PCR analysis revealed higher induction of StCuZnSODs (the major potato SODs) and StFeSOD3 in thermotolerant cultivar Désirée than in thermosensitive Agria and Kennebec during long-term exposure to elevated temperature. StMnSOD was constitutively expressed, while expression of StFeSODs was cultivar-dependent. The effects of salicylic acid (10−5 M) on StSODs expression were minor. Our results provide the basis for further research on StSODs and their regulation in potato, particularly in response to elevated temperatures.",
journal = "Antioxidants",
title = "In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression",
number = "3",
volume = "11",
doi = "10.3390/antiox11030488",
pages = "488"
}

Rudić, J., Dragićević, M., Momčilović, I., Simonović, A.,& Pantelić, D.. (2022). In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression. in Antioxidants, 11(3), 488.
https://doi.org/10.3390/antiox11030488

Rudić J, Dragićević M, Momčilović I, Simonović A, Pantelić D. In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression. in Antioxidants. 2022;11(3):488.
doi:10.3390/antiox11030488 .

Rudić, Jelena, Dragićević, Milan, Momčilović, Ivana, Simonović, Ana, Pantelić, Danijel, "In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression" in Antioxidants, 11, no. 3 (2022):488,
https://doi.org/10.3390/antiox11030488 . .

TY  - JOUR
AU  - Erić, Pavle
AU  - Stamenković-Radak, Marina
AU  - Dragićević, Milan
AU  - Kankare, Maaria
AU  - Wallace, Megan A.
AU  - Savić Veselinović, Marija
AU  - Jelić, Mihailo
PY  - 2022
UR  - http://www.eje.cz/doi/10.14411/eje.2022.011.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4872
AB  - Drosophila obscura is a common fruit fly that inhabits the temperate forests of Europe. While it is abundant in the north compared to other Drosophila, its density decreases southwards, where it is gradually replaced by other Drosophila species. This study describes variation in the mitochondrial Cyt b gene of D. obscura from several European populations. We observed a large number of haplotypes, together with the structuring of genetic variation. Genetic variation is higher in the west where O1 and related divergent haplotypes dominate. In the east, the O2 haplotype is most frequent, together with haplotypes that recently arose from it. In the central part of the species range, both O1 and O2 are equally present, along with many others. These data reveal signs of population expansions that probably happened earlier in the west, and more recently in the east. Though our conclusions are based on only one genetic marker, limiting the power of the analysis, the results imply either postglacial expansion from two unique sources or, more likely, eastwards stepping-stone expansion. This study adds important information on genetic variation and phylogeography to the obscure biology of D. obscura, a species that has the potential to become an interesting model in evolutionary biology and conservation genetics.
PB  - České Budějovice: Czech Academy of Sciences
T2  - European Journal of Entomology
T1  - Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe
VL  - 119
DO  - 10.14411/eje.2022.011
SP  - 99
EP  - 110
ER  - 

@article{
author = "Erić, Pavle and Stamenković-Radak, Marina and Dragićević, Milan and Kankare, Maaria and Wallace, Megan A. and Savić Veselinović, Marija and Jelić, Mihailo",
year = "2022",
abstract = "Drosophila obscura is a common fruit fly that inhabits the temperate forests of Europe. While it is abundant in the north compared to other Drosophila, its density decreases southwards, where it is gradually replaced by other Drosophila species. This study describes variation in the mitochondrial Cyt b gene of D. obscura from several European populations. We observed a large number of haplotypes, together with the structuring of genetic variation. Genetic variation is higher in the west where O1 and related divergent haplotypes dominate. In the east, the O2 haplotype is most frequent, together with haplotypes that recently arose from it. In the central part of the species range, both O1 and O2 are equally present, along with many others. These data reveal signs of population expansions that probably happened earlier in the west, and more recently in the east. Though our conclusions are based on only one genetic marker, limiting the power of the analysis, the results imply either postglacial expansion from two unique sources or, more likely, eastwards stepping-stone expansion. This study adds important information on genetic variation and phylogeography to the obscure biology of D. obscura, a species that has the potential to become an interesting model in evolutionary biology and conservation genetics.",
publisher = "České Budějovice: Czech Academy of Sciences",
journal = "European Journal of Entomology",
title = "Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe",
volume = "119",
doi = "10.14411/eje.2022.011",
pages = "99-110"
}

Erić, P., Stamenković-Radak, M., Dragićević, M., Kankare, M., Wallace, M. A., Savić Veselinović, M.,& Jelić, M.. (2022). Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe. in European Journal of Entomology
České Budějovice: Czech Academy of Sciences., 119, 99-110.
https://doi.org/10.14411/eje.2022.011

Erić P, Stamenković-Radak M, Dragićević M, Kankare M, Wallace MA, Savić Veselinović M, Jelić M. Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe. in European Journal of Entomology. 2022;119:99-110.
doi:10.14411/eje.2022.011 .

Erić, Pavle, Stamenković-Radak, Marina, Dragićević, Milan, Kankare, Maaria, Wallace, Megan A., Savić Veselinović, Marija, Jelić, Mihailo, "Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe" in European Journal of Entomology, 119 (2022):99-110,
https://doi.org/10.14411/eje.2022.011 . .

TY  - CONF
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Dragićević, Milan
AU  - Filipović, Biljana
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5711
AB  - Кичица (Centaurium erythraea Rafn) представља богат извор фармаколошки активних специјализованих метаболита, од којих су најзаступљенији секоиридоиди. Биосинтеза и разградња секоиридоида код кичице су координисани процеси који обезбеђују конститутивно присуство ових материја у ткивима биљке. Скелет молекула секоиридоидних глукозида се заснива на циклопентан-C-пирану за који је, на позицији C1, везан молекул β-D глукозе. У реакцији секоиридоидних глукозида са хидролитичким ензимима ослобађају се одговарајући агликони који се даље могу укључити у метаболичке процесе биљке. Код кичице су уочене две варијанте ензима бета-глукозидаза које припадају ензимској породици глукозидних хидролаза 1 (GH1). Гени за ензиме CeBGLU1 и CeBGLU2 су хетеролого експримирани у организму домаћину (Escherichia coli), а након изолације и пречишћавања рекомбинантних протеина, урађени су in vitro ензиматски тестови за потврду фунције ензима. Обе изоформе ензима показују високу специфичност за секоиридоидне глукозиде као супстрат чиме је по први пут потврђена функција биљних бета-глукозидаза карактеристичних за ову групу једињења. Привремена експресија CeBGLU1 и CeBGLU2 у листовима кичице, техником агроинфилтрације, довела је до редукције садржаја секоиридоида у листoвима, у случајевима када су CeBGLU1 и CeBGLU2 ко-експримирани заједно са p19 супресором. На тај начин је додатно потврђена функција два ензима, за које је утврђено да каталишу почетни корак у катаболизму секоиридоидних глукоида код кичице.
AB  - Kičica (Centaurium erythraea Rafn) predstavlja bogat izvor farmakološki aktivnih specijalizovanih metabolita, od kojih su najzastupljeniji sekoiridoidi. Biosinteza i razgradnja sekoiridoida kod kičice su koordinisani procesi koji obezbeđuju konstitutivno prisustvo ovih materija u tkivima biljke. Skelet molekula sekoiridoidnih glukozida se zasniva na ciklopentan-C-piranu za koji je, na poziciji C1, vezan molekul β-D glukoze. U reakciji sekoiridoidnih glukozida sa hidrolitičkim enzimima oslobađaju se odgovarajući aglikoni koji se dalje mogu uključiti u metaboličke procese biljke. Kod kičice su uočene dve varijante enzima beta-glukozidaza koje pripadaju enzimskoj porodici glukozidnih hidrolaza 1 (GH1). Geni za enzime CeBGLU1 i CeBGLU2 su heterologo eksprimirani u organizmu domaćinu (Escherichia coli), a nakon izolacije i prečišćavanja rekombinantnih proteina, urađeni su in vitro enzimatski testovi za potvrdu funcije enzima. Obe izoforme enzima pokazuju visoku specifičnost za sekoiridoidne glukozide kao supstrat čime je po prvi put potvrđena funkcija biljnih beta-glukozidaza karakterističnih za ovu grupu jedinjenja. Privremena ekspresija CeBGLU1 i CeBGLU2 u listovima kičice, tehnikom agroinfiltracije, dovela je do redukcije sadržaja sekoiridoida u listovima, u slučajevima kada su CeBGLU1 i CeBGLU2 ko-eksprimirani zajedno sa p19 supresorom. Na taj način je dodatno potvrđena funkcija dva enzima, za koje je utvrđeno da katališu početni korak u katabolizmu sekoiridoidnih glukoida kod kičice.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Изолација и функционална карактеризација гена за бета- глукозидазу врсте Centaurium erythraea Rafn
T1  - Izolacija i funkcionalna karakterizacija gena za beta- glukozidazu vrste Centaurium erythraea Rafn
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5711
ER  - 

@conference{
author = "Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Živković, Suzana and Skorić, Marijana and Matekalo, Dragana and Dragićević, Milan and Filipović, Biljana and Mišić, Danijela",
year = "2022",
abstract = "Кичица (Centaurium erythraea Rafn) представља богат извор фармаколошки активних специјализованих метаболита, од којих су најзаступљенији секоиридоиди. Биосинтеза и разградња секоиридоида код кичице су координисани процеси који обезбеђују конститутивно присуство ових материја у ткивима биљке. Скелет молекула секоиридоидних глукозида се заснива на циклопентан-C-пирану за који је, на позицији C1, везан молекул β-D глукозе. У реакцији секоиридоидних глукозида са хидролитичким ензимима ослобађају се одговарајући агликони који се даље могу укључити у метаболичке процесе биљке. Код кичице су уочене две варијанте ензима бета-глукозидаза које припадају ензимској породици глукозидних хидролаза 1 (GH1). Гени за ензиме CeBGLU1 и CeBGLU2 су хетеролого експримирани у организму домаћину (Escherichia coli), а након изолације и пречишћавања рекомбинантних протеина, урађени су in vitro ензиматски тестови за потврду фунције ензима. Обе изоформе ензима показују високу специфичност за секоиридоидне глукозиде као супстрат чиме је по први пут потврђена функција биљних бета-глукозидаза карактеристичних за ову групу једињења. Привремена експресија CeBGLU1 и CeBGLU2 у листовима кичице, техником агроинфилтрације, довела је до редукције садржаја секоиридоида у листoвима, у случајевима када су CeBGLU1 и CeBGLU2 ко-експримирани заједно са p19 супресором. На тај начин је додатно потврђена функција два ензима, за које је утврђено да каталишу почетни корак у катаболизму секоиридоидних глукоида код кичице., Kičica (Centaurium erythraea Rafn) predstavlja bogat izvor farmakološki aktivnih specijalizovanih metabolita, od kojih su najzastupljeniji sekoiridoidi. Biosinteza i razgradnja sekoiridoida kod kičice su koordinisani procesi koji obezbeđuju konstitutivno prisustvo ovih materija u tkivima biljke. Skelet molekula sekoiridoidnih glukozida se zasniva na ciklopentan-C-piranu za koji je, na poziciji C1, vezan molekul β-D glukoze. U reakciji sekoiridoidnih glukozida sa hidrolitičkim enzimima oslobađaju se odgovarajući aglikoni koji se dalje mogu uključiti u metaboličke procese biljke. Kod kičice su uočene dve varijante enzima beta-glukozidaza koje pripadaju enzimskoj porodici glukozidnih hidrolaza 1 (GH1). Geni za enzime CeBGLU1 i CeBGLU2 su heterologo eksprimirani u organizmu domaćinu (Escherichia coli), a nakon izolacije i prečišćavanja rekombinantnih proteina, urađeni su in vitro enzimatski testovi za potvrdu funcije enzima. Obe izoforme enzima pokazuju visoku specifičnost za sekoiridoidne glukozide kao supstrat čime je po prvi put potvrđena funkcija biljnih beta-glukozidaza karakterističnih za ovu grupu jedinjenja. Privremena ekspresija CeBGLU1 i CeBGLU2 u listovima kičice, tehnikom agroinfiltracije, dovela je do redukcije sadržaja sekoiridoida u listovima, u slučajevima kada su CeBGLU1 i CeBGLU2 ko-eksprimirani zajedno sa p19 supresorom. Na taj način je dodatno potvrđena funkcija dva enzima, za koje je utvrđeno da katališu početni korak u katabolizmu sekoiridoidnih glukoida kod kičice.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Изолација и функционална карактеризација гена за бета- глукозидазу врсте Centaurium erythraea Rafn, Izolacija i funkcionalna karakterizacija gena za beta- glukozidazu vrste Centaurium erythraea Rafn",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5711"
}

Božunović, J., Milutinović, M., Aničić, N., Živković, S., Skorić, M., Matekalo, D., Dragićević, M., Filipović, B.,& Mišić, D.. (2022). Изолација и функционална карактеризација гена за бета- глукозидазу врсте Centaurium erythraea Rafn. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 34.
https://hdl.handle.net/21.15107/rcub_ibiss_5711

Božunović J, Milutinović M, Aničić N, Živković S, Skorić M, Matekalo D, Dragićević M, Filipović B, Mišić D. Изолација и функционална карактеризација гена за бета- глукозидазу врсте Centaurium erythraea Rafn. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:34.
https://hdl.handle.net/21.15107/rcub_ibiss_5711 .

Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Živković, Suzana, Skorić, Marijana, Matekalo, Dragana, Dragićević, Milan, Filipović, Biljana, Mišić, Danijela, "Изолација и функционална карактеризација гена за бета- глукозидазу врсте Centaurium erythraea Rafn" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):34,
https://hdl.handle.net/21.15107/rcub_ibiss_5711 .

TY  - CONF
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Živković, Suzana
AU  - Dragićević, Milan
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Filipović, Biljana
AU  - Petrović, Luka
AU  - Banjanac, Tijana
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5189
AB  - Centaurium erythraea Rafn, also known as common centaury, is a rich source of pharmacologically active specialized metabolites among which secoiridoid glucosides (SG) predominate. These compounds belong to the group of monoterpenoids, which are derived from the iridoid cyclopentan-C-pyran skeleton with β-D glucose linked at C1 position. Major SGs of C. erythraea (sweroside, swertiamarin, and gentiopicrin) are biosynthesized through general iridoid pathway via secologanin, and it has been recently proved that their catabolism starts with the deglycosylation step catalyzed by beta-glucosidases (BGlu). Two BGlu genes from C. erythraea (CeBGlu1 and CeBGlu2), with previously confirmed function, were transiently overexpressed in leaves of five month-old diploid and tetraploid centaury plants. Agroinfiltration of Agrobacterium tumefaciens GV3101 strain harboring genes of interest in TMV-based overexpression pJL-TRBO vector (pJL-TR-BO:CeBGlu1; pJL-TRBO:CeBGlu2) resulted in transient in planta overexpression of CeBGlu1 and Ce-BGlu2. Metabolic profiling of secoiridoids in leaves of diploid and tetraploid C. erythraea genotypes revealed that amounts of sweroside, swertiamarin, and gentiopicrin, were significantly reduced in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. Diploid plants displayed more pronounced decrease in SGs content than tetraploids. The effectiveness of gene overexpression was obviously altered by plant ploidy, and was under the control of post-transcriptional gene silencing mechanisms in the infiltrated tissues. Transgenes are more prone to transcriptional inactivation in C. erythraea tetraploids than in diploids. In conclusion, SGs-specific β-glucosidases could serve as a molecular target of biotechnological interest, in order to shape SG profiles of centaury and related economically important
species of the Gentianaceae family.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5189
ER  - 

@conference{
author = "Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Živković, Suzana and Dragićević, Milan and Skorić, Marijana and Matekalo, Dragana and Filipović, Biljana and Petrović, Luka and Banjanac, Tijana and Mišić, Danijela",
year = "2022",
abstract = "Centaurium erythraea Rafn, also known as common centaury, is a rich source of pharmacologically active specialized metabolites among which secoiridoid glucosides (SG) predominate. These compounds belong to the group of monoterpenoids, which are derived from the iridoid cyclopentan-C-pyran skeleton with β-D glucose linked at C1 position. Major SGs of C. erythraea (sweroside, swertiamarin, and gentiopicrin) are biosynthesized through general iridoid pathway via secologanin, and it has been recently proved that their catabolism starts with the deglycosylation step catalyzed by beta-glucosidases (BGlu). Two BGlu genes from C. erythraea (CeBGlu1 and CeBGlu2), with previously confirmed function, were transiently overexpressed in leaves of five month-old diploid and tetraploid centaury plants. Agroinfiltration of Agrobacterium tumefaciens GV3101 strain harboring genes of interest in TMV-based overexpression pJL-TRBO vector (pJL-TR-BO:CeBGlu1; pJL-TRBO:CeBGlu2) resulted in transient in planta overexpression of CeBGlu1 and Ce-BGlu2. Metabolic profiling of secoiridoids in leaves of diploid and tetraploid C. erythraea genotypes revealed that amounts of sweroside, swertiamarin, and gentiopicrin, were significantly reduced in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. Diploid plants displayed more pronounced decrease in SGs content than tetraploids. The effectiveness of gene overexpression was obviously altered by plant ploidy, and was under the control of post-transcriptional gene silencing mechanisms in the infiltrated tissues. Transgenes are more prone to transcriptional inactivation in C. erythraea tetraploids than in diploids. In conclusion, SGs-specific β-glucosidases could serve as a molecular target of biotechnological interest, in order to shape SG profiles of centaury and related economically important
species of the Gentianaceae family.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5189"
}

Božunović, J., Milutinović, M., Aničić, N., Živković, S., Dragićević, M., Skorić, M., Matekalo, D., Filipović, B., Petrović, L., Banjanac, T.,& Mišić, D.. (2022). Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 109.
https://hdl.handle.net/21.15107/rcub_ibiss_5189

Božunović J, Milutinović M, Aničić N, Živković S, Dragićević M, Skorić M, Matekalo D, Filipović B, Petrović L, Banjanac T, Mišić D. Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:109.
https://hdl.handle.net/21.15107/rcub_ibiss_5189 .

Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Živković, Suzana, Dragićević, Milan, Skorić, Marijana, Matekalo, Dragana, Filipović, Biljana, Petrović, Luka, Banjanac, Tijana, Mišić, Danijela, "Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):109,
https://hdl.handle.net/21.15107/rcub_ibiss_5189 .

TY  - CONF
AU  - Šajkunić, Sanja
AU  - Dragićević, Milan
AU  - Milutinović, Milica
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Devrnja, Nina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5050
AB  - Different stressors from the environment can prime defense and enable enhanced responses
to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the
level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown
promising prospects as novel priming inducers. Still, little is known about their potential to induce
epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula
L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to
EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal.
Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM)
DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR,
and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and
treated samples were analyzed. The average methylation level of the whole genome was 72.29%
and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly
occurred in the CG context compared to CHH and CHG. In general, hypomethylated
sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially
methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein
processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes
associated with these pathways, as well as different defense responses in potato (GO:0006952),
will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress
conditions.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - French marigold essential oil affected DNA methylation in potato
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5050
ER  - 

@conference{
author = "Šajkunić, Sanja and Dragićević, Milan and Milutinović, Milica and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena and Devrnja, Nina",
year = "2022",
abstract = "Different stressors from the environment can prime defense and enable enhanced responses
to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the
level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown
promising prospects as novel priming inducers. Still, little is known about their potential to induce
epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula
L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to
EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal.
Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM)
DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR,
and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and
treated samples were analyzed. The average methylation level of the whole genome was 72.29%
and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly
occurred in the CG context compared to CHH and CHG. In general, hypomethylated
sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially
methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein
processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes
associated with these pathways, as well as different defense responses in potato (GO:0006952),
will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress
conditions.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "French marigold essential oil affected DNA methylation in potato",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5050"
}

Šajkunić, S., Dragićević, M., Milutinović, M., Stupar, S., Tubić, L., Savić, J.,& Devrnja, N.. (2022). French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 71.
https://hdl.handle.net/21.15107/rcub_ibiss_5050

Šajkunić S, Dragićević M, Milutinović M, Stupar S, Tubić L, Savić J, Devrnja N. French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:71.
https://hdl.handle.net/21.15107/rcub_ibiss_5050 .

Šajkunić, Sanja, Dragićević, Milan, Milutinović, Milica, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, Devrnja, Nina, "French marigold essential oil affected DNA methylation in potato" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):71,
https://hdl.handle.net/21.15107/rcub_ibiss_5050 .

TY  - CONF
AU  - Šajkunić, Sanja
AU  - Dragićević, Milan
AU  - Milutinović, Milica
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Devrnja, Nina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5051
AB  - Different stressors from the environment can prime defense and enable enhanced responses to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown promising prospects as novel priming inducers. Still, little is known about their potential to induce epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM) DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and treated samples were analyzed. The average methylation level of the whole genome was 72.29% and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly occurred in the CG context compared to CHH and CHG. In general, hypomethylated sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes associated with these pathways, as well as different defense responses in potato (GO:0006952), will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress conditions.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.
T1  - French marigold essential oil affected DNA methylation in potato
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5051
ER  - 

@conference{
author = "Šajkunić, Sanja and Dragićević, Milan and Milutinović, Milica and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena and Devrnja, Nina",
year = "2022",
abstract = "Different stressors from the environment can prime defense and enable enhanced responses to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown promising prospects as novel priming inducers. Still, little is known about their potential to induce epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM) DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and treated samples were analyzed. The average methylation level of the whole genome was 72.29% and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly occurred in the CG context compared to CHH and CHG. In general, hypomethylated sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes associated with these pathways, as well as different defense responses in potato (GO:0006952), will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress conditions.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.",
title = "French marigold essential oil affected DNA methylation in potato",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5051"
}

Šajkunić, S., Dragićević, M., Milutinović, M., Stupar, S., Tubić, L., Savić, J.,& Devrnja, N.. (2022). French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5051

Šajkunić S, Dragićević M, Milutinović M, Stupar S, Tubić L, Savić J, Devrnja N. French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5051 .

Šajkunić, Sanja, Dragićević, Milan, Milutinović, Milica, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, Devrnja, Nina, "French marigold essential oil affected DNA methylation in potato" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5051 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Jelić, Mihailo
AU  - Marinković, Saša
AU  - Kukobat, Mila
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Sušić, Goran
AU  - Dragićević, Milan
AU  - Tanasković, Marija
AU  - Stamenković-Radak, Marina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4254
AB  - Abstract
Once a widespread species across the region of Southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction programs. The available genetic data for this valuable population are scarce and it is necessary to assess its genetic diversity and inbreeding level if the population is going to be used for restocking and reintroduction.
To assess the genetic diversity we used microsatellite markers from ten loci and mitochondrial Cytb nucleotide sequences. The blood samples were collected from 58 unrelated birds during the marking in the nests. We have performed a comparative analysis of newly obtained data on microsatellites and Cytb with existing data. Genetic differentiation analysis between different native populations of French Pyrenees, Croatia and Israel identified two genetic clusters that differentiate populations from the Balkan and Iberian Peninsulas. Genetic diversity analysis based on microsatellites demonstrated similar levels among all populations while analysis of Cytb detected somewhat lower diversity in the population from Serbia. Further analyses demonstrated that all analyzed populations experienced a recent bottleneck event. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Considering the serious recent bottleneck event which the population from Serbia experienced we estimated the overall relatedness among the birds from this population. The level of inbreeding was relatively high and on average it was 8,3% while the mean number of relatives for each bird was close to three.
Our data suggest that, even though a relatively high level of inbreeding can be detected among the individual birds, the Griffon vulture population from Serbia can be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsula suggest that the introduction of foreign birds should be avoided and that local birds should be used instead.
PB  - Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
C3  - Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
T1  - Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia
IS  - 1
VL  - 43
SP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4254
ER  - 

@conference{
author = "Davidović, Slobodan and Jelić, Mihailo and Marinković, Saša and Kukobat, Mila and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Sušić, Goran and Dragićević, Milan and Tanasković, Marija and Stamenković-Radak, Marina",
year = "2021",
abstract = "Abstract
Once a widespread species across the region of Southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction programs. The available genetic data for this valuable population are scarce and it is necessary to assess its genetic diversity and inbreeding level if the population is going to be used for restocking and reintroduction.
To assess the genetic diversity we used microsatellite markers from ten loci and mitochondrial Cytb nucleotide sequences. The blood samples were collected from 58 unrelated birds during the marking in the nests. We have performed a comparative analysis of newly obtained data on microsatellites and Cytb with existing data. Genetic differentiation analysis between different native populations of French Pyrenees, Croatia and Israel identified two genetic clusters that differentiate populations from the Balkan and Iberian Peninsulas. Genetic diversity analysis based on microsatellites demonstrated similar levels among all populations while analysis of Cytb detected somewhat lower diversity in the population from Serbia. Further analyses demonstrated that all analyzed populations experienced a recent bottleneck event. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Considering the serious recent bottleneck event which the population from Serbia experienced we estimated the overall relatedness among the birds from this population. The level of inbreeding was relatively high and on average it was 8,3% while the mean number of relatives for each bird was close to three.
Our data suggest that, even though a relatively high level of inbreeding can be detected among the individual birds, the Griffon vulture population from Serbia can be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsula suggest that the introduction of foreign birds should be avoided and that local birds should be used instead.",
publisher = "Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia",
title = "Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia",
number = "1",
volume = "43",
pages = "117",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4254"
}

Davidović, S., Jelić, M., Marinković, S., Kukobat, M., Mihajlović, M., Tanasić, V., Hribšek, I., Sušić, G., Dragićević, M., Tanasković, M.,& Stamenković-Radak, M.. (2021). Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43(1), 117.
https://hdl.handle.net/21.15107/rcub_ibiss_4254

Davidović S, Jelić M, Marinković S, Kukobat M, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Tanasković M, Stamenković-Radak M. Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia. 2021;43(1):117.
https://hdl.handle.net/21.15107/rcub_ibiss_4254 .

Davidović, Slobodan, Jelić, Mihailo, Marinković, Saša, Kukobat, Mila, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Sušić, Goran, Dragićević, Milan, Tanasković, Marija, Stamenković-Radak, Marina, "Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia" in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia, 43, no. 1 (2021):117,
https://hdl.handle.net/21.15107/rcub_ibiss_4254 .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragićević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Perišić, Mirjana
AU  - Mišić, Danijela
PY  - 2021
UR  - https://doi.org/10.3390/plants10010142
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4232
AB  - Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.
PB  - MDPI AG
T2  - Plants
T1  - Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro
IS  - 1
VL  - 10
DO  - 10.3390/plants10010142
SP  - 1
EP  - 20
ER  - 

@article{
author = "Dmitrović, Slavica and Dragićević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Perišić, Mirjana and Mišić, Danijela",
year = "2021",
abstract = "Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.",
publisher = "MDPI AG",
journal = "Plants",
title = "Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro",
number = "1",
volume = "10",
doi = "10.3390/plants10010142",
pages = "1-20"
}

Dmitrović, S., Dragićević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D., Perišić, M.,& Mišić, D.. (2021). Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro. in Plants
MDPI AG., 10(1), 1-20.
https://doi.org/10.3390/plants10010142

Dmitrović S, Dragićević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Perišić M, Mišić D. Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro. in Plants. 2021;10(1):1-20.
doi:10.3390/plants10010142 .

Dmitrović, Slavica, Dragićević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Perišić, Mirjana, Mišić, Danijela, "Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro" in Plants, 10, no. 1 (2021):1-20,
https://doi.org/10.3390/plants10010142 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Milošević, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4119
AB  - Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.
PB  - Basel, Switzerland: MDPI
T2  - Plants
T1  - Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery
IS  - 1
VL  - 10
DO  - 10.3390/plants10010154
SP  - 154
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Dragićević, Milan and Simonović, Ana and Milošević, Snežana",
year = "2021",
abstract = "Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.",
publisher = "Basel, Switzerland: MDPI",
journal = "Plants",
title = "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery",
number = "1",
volume = "10",
doi = "10.3390/plants10010154",
pages = "154"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M., Cingel, A., Dragićević, M., Simonović, A.,& Milošević, S.. (2021). Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants
Basel, Switzerland: MDPI., 10(1), 154.
https://doi.org/10.3390/plants10010154

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Cingel A, Dragićević M, Simonović A, Milošević S. Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants. 2021;10(1):154.
doi:10.3390/plants10010154 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Dragićević, Milan, Simonović, Ana, Milošević, Snežana, "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery" in Plants, 10, no. 1 (2021):154,
https://doi.org/10.3390/plants10010154 . .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Ćuković, Katarina
AU  - Subotić, Angelina
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Filipović, Biljana
AU  - Todorović, Slađana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4126
AB  - Somatic embryogenesis (SE) is a developmental process during which plant somatic cells, under suitable conditions, produce embryogenic cells that develop into somatic embryos (se). SE is the most important method for plant propagation in vitro, having both fundamental and applicative significance. SE can be induced from different tissues and organs, but when se are used as explants, the process is recognized as secondary or cyclic SE. We induced secondary SE in Centaurium erythraea by application of 2,4-dichlorophenoxyacetic acid (2,4-D) and N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU). A medium containing 0.1 mgL−1 2,4-D and 0.25 mgL−1 CPPU was optimal in terms of the number of primary SE explants forming se, the number of well-developed se per explant, and morphological appearance of the obtained se. These concentrations allowed SE to progress through three cycles, whereas at higher concentrations of 0.2 mgL−1 2,4-D and 0.5 mgL−1 CPPU, only two cycles were achieved. Histological analysis revealed that secondary se are formed both directly and indirectly. Secondary SE readily germinated and converted into plantlets. Induction of cyclic SE contributes to the conservation efforts of this endangered medicinal plant and expands the spectrum of in vitro developmental pathways described in centaury—an emerging model in developmental biology.
PB  - Basel : MDPI
T2  - Plants
T1  - Secondary Somatic Embryogenesis in Centaurium erythraea Rafn
IS  - 2
VL  - 10
DO  - 10.3390/plants10020199
SP  - 199
ER  - 

@article{
author = "Bogdanović, Milica and Ćuković, Katarina and Subotić, Angelina and Dragićević, Milan and Simonović, Ana and Filipović, Biljana and Todorović, Slađana",
year = "2021",
abstract = "Somatic embryogenesis (SE) is a developmental process during which plant somatic cells, under suitable conditions, produce embryogenic cells that develop into somatic embryos (se). SE is the most important method for plant propagation in vitro, having both fundamental and applicative significance. SE can be induced from different tissues and organs, but when se are used as explants, the process is recognized as secondary or cyclic SE. We induced secondary SE in Centaurium erythraea by application of 2,4-dichlorophenoxyacetic acid (2,4-D) and N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU). A medium containing 0.1 mgL−1 2,4-D and 0.25 mgL−1 CPPU was optimal in terms of the number of primary SE explants forming se, the number of well-developed se per explant, and morphological appearance of the obtained se. These concentrations allowed SE to progress through three cycles, whereas at higher concentrations of 0.2 mgL−1 2,4-D and 0.5 mgL−1 CPPU, only two cycles were achieved. Histological analysis revealed that secondary se are formed both directly and indirectly. Secondary SE readily germinated and converted into plantlets. Induction of cyclic SE contributes to the conservation efforts of this endangered medicinal plant and expands the spectrum of in vitro developmental pathways described in centaury—an emerging model in developmental biology.",
publisher = "Basel : MDPI",
journal = "Plants",
title = "Secondary Somatic Embryogenesis in Centaurium erythraea Rafn",
number = "2",
volume = "10",
doi = "10.3390/plants10020199",
pages = "199"
}

Bogdanović, M., Ćuković, K., Subotić, A., Dragićević, M., Simonović, A., Filipović, B.,& Todorović, S.. (2021). Secondary Somatic Embryogenesis in Centaurium erythraea Rafn. in Plants
Basel : MDPI., 10(2), 199.
https://doi.org/10.3390/plants10020199

Bogdanović M, Ćuković K, Subotić A, Dragićević M, Simonović A, Filipović B, Todorović S. Secondary Somatic Embryogenesis in Centaurium erythraea Rafn. in Plants. 2021;10(2):199.
doi:10.3390/plants10020199 .

Bogdanović, Milica, Ćuković, Katarina, Subotić, Angelina, Dragićević, Milan, Simonović, Ana, Filipović, Biljana, Todorović, Slađana, "Secondary Somatic Embryogenesis in Centaurium erythraea Rafn" in Plants, 10, no. 2 (2021):199,
https://doi.org/10.3390/plants10020199 . .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Stanković-Jeremić, Jovana
AU  - Maksimović, Vuk
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - internal-pdf://Stupar et al. - 2021 - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf St.pdf
UR  - https://www.mdpi.com/2223-7747/10/1/172
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4147
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI AG
T2  - Plants
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
SP  - 172
ER  - 

@article{
author = "Stupar, Sofija and Dragićević, Milan and Tešević, Vele and Stanković-Jeremić, Jovana and Maksimović, Vuk and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI AG",
journal = "Plants",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172",
pages = "172"
}

Stupar, S., Dragićević, M., Tešević, V., Stanković-Jeremić, J., Maksimović, V., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants
MDPI AG., 10(1), 172.
https://doi.org/10.3390/plants10010172

Stupar S, Dragićević M, Tešević V, Stanković-Jeremić J, Maksimović V, Ćosić T, Devrnja N, Tubić L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants. 2021;10(1):172.
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragićević, Milan, Tešević, Vele, Stanković-Jeremić, Jovana, Maksimović, Vuk, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants, 10, no. 1 (2021):172,
https://doi.org/10.3390/plants10010172 . .

TY  - JOUR
AU  - Aničić, Neda
AU  - Patelou, Efstathia
AU  - Papanikolaou, Antigoni
AU  - Kanioura, Anthi
AU  - Valdesturli, Camilla
AU  - Arapitsas, Panagiotis
AU  - Skorić, Marijana
AU  - Dragićević, Milan
AU  - Gašić, Uroš
AU  - Koukounaras, Athanasios
AU  - Kostas, Stefanos
AU  - Sarrou, Eirini
AU  - Martens, Stefan
AU  - Mišić, Danijela
AU  - Kanellis, Angelos
PY  - 2021
UR  - www.frontiersin.org
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4219
AB  - Cistus creticus L. subsp. creticus (rockrose) is a shrub widespread in Greece and the Mediterranean basin and has been used in traditional medicine as herb tea for colds, for healing and digestive hitches, for the treatment of maladies, as perfumes, and for other purposes. Compounds from its flavonoid fraction have recently drawn attention due to antiviral action against influenza virus and HIV. Although several bioactive metabolites belonging to this group have been chemically characterized in the leaves, the genes involved in their biosynthesis in Cistus remain largely unknown. Flavonoid metabolism during C. creticus fruit development was studied by adopting comparative metabolomic and transcriptomic approaches. The present study highlights the fruit of C. creticus subsp. creticus as a rich source of flavonols, flavan-3-ols, and proanthocyanidins, all of which displayed a decreasing trend during fruit development. The majority of proanthocyanidins recorded in Cistus fruit are B-type procyanidins and prodelphinidins, while gallocatechin and catechin are the dominant flavan-3-ols. The expression patterns of biosynthetic genes and transcription factors were analyzed in flowers and throughout three fruit development stages. Flavonoid biosynthetic genes were developmentally regulated, showing a decrease in transcript levels during fruit maturation. A high degree of positive correlations between the content of targeted metabolites and the expression of biosynthetic genes indicated the transcriptional regulation of flavonoid biosynthesis during C. creticus fruit development. This is further supported by the high degree of significant positive correlations between the expression of biosynthetic genes and transcription factors. The results suggest that leucoanthocyanidin reductase predominates the biosynthetic pathway in the control of flavan-3-ol formation, which results in catechin and gallocatechin as two of the major building blocks for Cistus proanthocyanidins. Additionally, there is a decline in ethylene production rates during non-climacteric Cistus fruit maturation, which coincides with the downregulation of the majority of flavonoid- and ethylene-related biosynthetic genes and corresponding transcription factors as well as with the decline in flavonoid content. Finally, functional characterization of a Cistus flavonoid hydroxylase (F3′5′H) was performed for the first time.
PB  - Frontiers Media S.A.
T2  - Frontiers in Plant Science
T1  - Comparative Metabolite and Gene Expression Analyses in Combination With Gene Characterization Revealed the Patterns of Flavonoid Accumulation During Cistus creticus subsp. creticus Fruit Development
VL  - 12
DO  - 10.3389/fpls.2021.619634
SP  - 385
ER  - 

@article{
author = "Aničić, Neda and Patelou, Efstathia and Papanikolaou, Antigoni and Kanioura, Anthi and Valdesturli, Camilla and Arapitsas, Panagiotis and Skorić, Marijana and Dragićević, Milan and Gašić, Uroš and Koukounaras, Athanasios and Kostas, Stefanos and Sarrou, Eirini and Martens, Stefan and Mišić, Danijela and Kanellis, Angelos",
year = "2021",
abstract = "Cistus creticus L. subsp. creticus (rockrose) is a shrub widespread in Greece and the Mediterranean basin and has been used in traditional medicine as herb tea for colds, for healing and digestive hitches, for the treatment of maladies, as perfumes, and for other purposes. Compounds from its flavonoid fraction have recently drawn attention due to antiviral action against influenza virus and HIV. Although several bioactive metabolites belonging to this group have been chemically characterized in the leaves, the genes involved in their biosynthesis in Cistus remain largely unknown. Flavonoid metabolism during C. creticus fruit development was studied by adopting comparative metabolomic and transcriptomic approaches. The present study highlights the fruit of C. creticus subsp. creticus as a rich source of flavonols, flavan-3-ols, and proanthocyanidins, all of which displayed a decreasing trend during fruit development. The majority of proanthocyanidins recorded in Cistus fruit are B-type procyanidins and prodelphinidins, while gallocatechin and catechin are the dominant flavan-3-ols. The expression patterns of biosynthetic genes and transcription factors were analyzed in flowers and throughout three fruit development stages. Flavonoid biosynthetic genes were developmentally regulated, showing a decrease in transcript levels during fruit maturation. A high degree of positive correlations between the content of targeted metabolites and the expression of biosynthetic genes indicated the transcriptional regulation of flavonoid biosynthesis during C. creticus fruit development. This is further supported by the high degree of significant positive correlations between the expression of biosynthetic genes and transcription factors. The results suggest that leucoanthocyanidin reductase predominates the biosynthetic pathway in the control of flavan-3-ol formation, which results in catechin and gallocatechin as two of the major building blocks for Cistus proanthocyanidins. Additionally, there is a decline in ethylene production rates during non-climacteric Cistus fruit maturation, which coincides with the downregulation of the majority of flavonoid- and ethylene-related biosynthetic genes and corresponding transcription factors as well as with the decline in flavonoid content. Finally, functional characterization of a Cistus flavonoid hydroxylase (F3′5′H) was performed for the first time.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Plant Science",
title = "Comparative Metabolite and Gene Expression Analyses in Combination With Gene Characterization Revealed the Patterns of Flavonoid Accumulation During Cistus creticus subsp. creticus Fruit Development",
volume = "12",
doi = "10.3389/fpls.2021.619634",
pages = "385"
}

Aničić, N., Patelou, E., Papanikolaou, A., Kanioura, A., Valdesturli, C., Arapitsas, P., Skorić, M., Dragićević, M., Gašić, U., Koukounaras, A., Kostas, S., Sarrou, E., Martens, S., Mišić, D.,& Kanellis, A.. (2021). Comparative Metabolite and Gene Expression Analyses in Combination With Gene Characterization Revealed the Patterns of Flavonoid Accumulation During Cistus creticus subsp. creticus Fruit Development. in Frontiers in Plant Science
Frontiers Media S.A.., 12, 385.
https://doi.org/10.3389/fpls.2021.619634

Aničić N, Patelou E, Papanikolaou A, Kanioura A, Valdesturli C, Arapitsas P, Skorić M, Dragićević M, Gašić U, Koukounaras A, Kostas S, Sarrou E, Martens S, Mišić D, Kanellis A. Comparative Metabolite and Gene Expression Analyses in Combination With Gene Characterization Revealed the Patterns of Flavonoid Accumulation During Cistus creticus subsp. creticus Fruit Development. in Frontiers in Plant Science. 2021;12:385.
doi:10.3389/fpls.2021.619634 .

Aničić, Neda, Patelou, Efstathia, Papanikolaou, Antigoni, Kanioura, Anthi, Valdesturli, Camilla, Arapitsas, Panagiotis, Skorić, Marijana, Dragićević, Milan, Gašić, Uroš, Koukounaras, Athanasios, Kostas, Stefanos, Sarrou, Eirini, Martens, Stefan, Mišić, Danijela, Kanellis, Angelos, "Comparative Metabolite and Gene Expression Analyses in Combination With Gene Characterization Revealed the Patterns of Flavonoid Accumulation During Cistus creticus subsp. creticus Fruit Development" in Frontiers in Plant Science, 12 (2021):385,
https://doi.org/10.3389/fpls.2021.619634 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Pejić, Snežana
AU  - Grigorov, Ilijana
AU  - Nikolić, Gorana
AU  - Mitić-Ćulafić, Dragana
AU  - Dragićević, Milan
AU  - Đorđević, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Đorđević, Neda
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Veljković, Filip
AU  - Pajović, Snežana B.
AU  - Todorović, Zoran
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4289
AB  - Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.
PB  - MDPI
T2  - Antioxidants
T1  - Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes
IS  - 6
VL  - 10
DO  - 10.3390/antiox10060911
SP  - 911
ER  - 

@article{
author = "Đurašević, Siniša and Pejić, Snežana and Grigorov, Ilijana and Nikolić, Gorana and Mitić-Ćulafić, Dragana and Dragićević, Milan and Đorđević, Jelena and Todorović Vukotić, Nevena and Đorđević, Neda and Todorović, Ana and Drakulić, Dunja and Veljković, Filip and Pajović, Snežana B. and Todorović, Zoran",
year = "2021",
abstract = "Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes",
number = "6",
volume = "10",
doi = "10.3390/antiox10060911",
pages = "911"
}

Đurašević, S., Pejić, S., Grigorov, I., Nikolić, G., Mitić-Ćulafić, D., Dragićević, M., Đorđević, J., Todorović Vukotić, N., Đorđević, N., Todorović, A., Drakulić, D., Veljković, F., Pajović, S. B.,& Todorović, Z.. (2021). Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants
MDPI., 10(6), 911.
https://doi.org/10.3390/antiox10060911

Đurašević S, Pejić S, Grigorov I, Nikolić G, Mitić-Ćulafić D, Dragićević M, Đorđević J, Todorović Vukotić N, Đorđević N, Todorović A, Drakulić D, Veljković F, Pajović SB, Todorović Z. Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants. 2021;10(6):911.
doi:10.3390/antiox10060911 .

Đurašević, Siniša, Pejić, Snežana, Grigorov, Ilijana, Nikolić, Gorana, Mitić-Ćulafić, Dragana, Dragićević, Milan, Đorđević, Jelena, Todorović Vukotić, Nevena, Đorđević, Neda, Todorović, Ana, Drakulić, Dunja, Veljković, Filip, Pajović, Snežana B., Todorović, Zoran, "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes" in Antioxidants, 10, no. 6 (2021):911,
https://doi.org/10.3390/antiox10060911 . .

TY  - JOUR
AU  - Paunović, Danijela
AU  - Ćuković, Katarina
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Subotić, Angelina
AU  - Simonović, Ana
AU  - Dragićević, Milan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4392
AB  - Centaurium erythraea (centaury) is a medicinal plant with exceptional developmental plasticity in vitro and vigorous, often spontaneous, regeneration via shoot organogenesis and somatic embryogenesis, during which arabinogalactan proteins (AGPs) play an important role. AGPs are highly glycosylated proteins belonging to the super family of O-glycosylated plant cell surface hydroxyproline-rich glycoproteins (HRGPs). HRGPs/AGPs are intrinsically disordered and not well conserved, making their homology-based mining ineffective. We have applied a recently developed pipeline for HRGP/AGP mining, ragp, which is based on machine learning prediction of proline hydroxylation, to identify HRGP sequences in centaury transcriptome and to classify them into motif and amino acid bias (MAAB) classes. AGP sequences with low AG glycomotif representation were also identified. Six members of each of the three AGP subclasses, fasciclin-like AGPs, receptor kinase-like AGPs and AG peptides, were selected for phylogenetic and expression analyses. The expression of these 18 genes was recorded over 48 h following leaf mechanical wounding, as well as in 16 tissue samples representing plants from nature, plants cultivated in vitro, and developmental stages during shoot organogenesis and somatic embryogenesis. None of the selected genes were upregulated during both wounding recovery and regeneration. Possible functions of AGPs with the most interesting expression profiles are discussed.
PB  - Basel: MDPI
T2  - Plants
T1  - The Arabinogalactan Protein Family of Centaurium erythraea Rafn
IS  - 9
VL  - 10
DO  - 10.3390/plants10091870
SP  - 1870
ER  - 

@article{
author = "Paunović, Danijela and Ćuković, Katarina and Bogdanović, Milica and Todorović, Slađana and Trifunović-Momčilov, Milana and Subotić, Angelina and Simonović, Ana and Dragićević, Milan",
year = "2021",
abstract = "Centaurium erythraea (centaury) is a medicinal plant with exceptional developmental plasticity in vitro and vigorous, often spontaneous, regeneration via shoot organogenesis and somatic embryogenesis, during which arabinogalactan proteins (AGPs) play an important role. AGPs are highly glycosylated proteins belonging to the super family of O-glycosylated plant cell surface hydroxyproline-rich glycoproteins (HRGPs). HRGPs/AGPs are intrinsically disordered and not well conserved, making their homology-based mining ineffective. We have applied a recently developed pipeline for HRGP/AGP mining, ragp, which is based on machine learning prediction of proline hydroxylation, to identify HRGP sequences in centaury transcriptome and to classify them into motif and amino acid bias (MAAB) classes. AGP sequences with low AG glycomotif representation were also identified. Six members of each of the three AGP subclasses, fasciclin-like AGPs, receptor kinase-like AGPs and AG peptides, were selected for phylogenetic and expression analyses. The expression of these 18 genes was recorded over 48 h following leaf mechanical wounding, as well as in 16 tissue samples representing plants from nature, plants cultivated in vitro, and developmental stages during shoot organogenesis and somatic embryogenesis. None of the selected genes were upregulated during both wounding recovery and regeneration. Possible functions of AGPs with the most interesting expression profiles are discussed.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "The Arabinogalactan Protein Family of Centaurium erythraea Rafn",
number = "9",
volume = "10",
doi = "10.3390/plants10091870",
pages = "1870"
}

Paunović, D., Ćuković, K., Bogdanović, M., Todorović, S., Trifunović-Momčilov, M., Subotić, A., Simonović, A.,& Dragićević, M.. (2021). The Arabinogalactan Protein Family of Centaurium erythraea Rafn. in Plants
Basel: MDPI., 10(9), 1870.
https://doi.org/10.3390/plants10091870

Paunović D, Ćuković K, Bogdanović M, Todorović S, Trifunović-Momčilov M, Subotić A, Simonović A, Dragićević M. The Arabinogalactan Protein Family of Centaurium erythraea Rafn. in Plants. 2021;10(9):1870.
doi:10.3390/plants10091870 .

Paunović, Danijela, Ćuković, Katarina, Bogdanović, Milica, Todorović, Slađana, Trifunović-Momčilov, Milana, Subotić, Angelina, Simonović, Ana, Dragićević, Milan, "The Arabinogalactan Protein Family of Centaurium erythraea Rafn" in Plants, 10, no. 9 (2021):1870,
https://doi.org/10.3390/plants10091870 . .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Simonović, Ana
PY  - 2021
UR  - https://ojs.pmf.uns.ac.rs/index.php/dbe_serbica/article/view/13522
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4423
AB  - Centaurium erythraea (common centaury) is a medicinal plant with extraordinary developmental plasticity
in vitro that is used as a model organism for studying in vitro morphogenesis in our lab. Several experimental lines of evidence have identified arabinogalactan proteins (AGPs) as one of the key players involved in centaury morphogenesis; however, the role of specific genes has yet to be determined. AGPs are ubiquitous plant cell surface glycoproteins associated with various physiological functions. AGP sequences are characterized by the presence of non-continuous hydroxyproline residues, which serve as O-glycosylation anchor sites for branched arabinogalactans. Due to a biased amino acid composition rich in disorder-promoting amino acids, AGP sequences lack a stable structure and consequently have lessened evolutionary constraints. Therefore, homology-based approaches to AGP sequence mining have limited success. We have recently developed a bioinformatics pipeline for AGP sequence mining, ragp, which exploits their key feature – the presence of hydroxyprolines. This pipeline combines estimation
of proline hydroxylation based on local sequence context by a machine learning model with a flexible motif search. After applying this pipeline to the centaury transcriptome, AGP regions were found to associate with a variety of conserved domains. Here we introduce a streamlined way to train models for prediction of Pro hydroxylation, analyze important protein sequence features determining Pro hydroxylation status, present some of the AGP types found in centaury and discuss model limitations and future prospects.
PB  - Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
T2  - Biologia Serbica
T1  - Arabinogalactan protein mining and diversity - the case of Centaurium erythraea
IS  - 1
VL  - 43
DO  - 10.5281/zenodo.5512429
SP  - 4
EP  - 11
ER  - 

@article{
author = "Dragićević, Milan and Simonović, Ana",
year = "2021",
abstract = "Centaurium erythraea (common centaury) is a medicinal plant with extraordinary developmental plasticity
in vitro that is used as a model organism for studying in vitro morphogenesis in our lab. Several experimental lines of evidence have identified arabinogalactan proteins (AGPs) as one of the key players involved in centaury morphogenesis; however, the role of specific genes has yet to be determined. AGPs are ubiquitous plant cell surface glycoproteins associated with various physiological functions. AGP sequences are characterized by the presence of non-continuous hydroxyproline residues, which serve as O-glycosylation anchor sites for branched arabinogalactans. Due to a biased amino acid composition rich in disorder-promoting amino acids, AGP sequences lack a stable structure and consequently have lessened evolutionary constraints. Therefore, homology-based approaches to AGP sequence mining have limited success. We have recently developed a bioinformatics pipeline for AGP sequence mining, ragp, which exploits their key feature – the presence of hydroxyprolines. This pipeline combines estimation
of proline hydroxylation based on local sequence context by a machine learning model with a flexible motif search. After applying this pipeline to the centaury transcriptome, AGP regions were found to associate with a variety of conserved domains. Here we introduce a streamlined way to train models for prediction of Pro hydroxylation, analyze important protein sequence features determining Pro hydroxylation status, present some of the AGP types found in centaury and discuss model limitations and future prospects.",
publisher = "Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Biologia Serbica",
title = "Arabinogalactan protein mining and diversity - the case of Centaurium erythraea",
number = "1",
volume = "43",
doi = "10.5281/zenodo.5512429",
pages = "4-11"
}

Dragićević, M.,& Simonović, A.. (2021). Arabinogalactan protein mining and diversity - the case of Centaurium erythraea. in Biologia Serbica
Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43(1), 4-11.
https://doi.org/10.5281/zenodo.5512429

Dragićević M, Simonović A. Arabinogalactan protein mining and diversity - the case of Centaurium erythraea. in Biologia Serbica. 2021;43(1):4-11.
doi:10.5281/zenodo.5512429 .

Dragićević, Milan, Simonović, Ana, "Arabinogalactan protein mining and diversity - the case of Centaurium erythraea" in Biologia Serbica, 43, no. 1 (2021):4-11,
https://doi.org/10.5281/zenodo.5512429 . .

TY  - JOUR
AU  - Trajković, Milena
AU  - Jevremović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Subotić, Angelina
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4434
AB  - Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis
IS  - 9
VL  - 7
DO  - 10.3390/horticulturae7090324
SP  - 324
ER  - 

@article{
author = "Trajković, Milena and Jevremović, Slađana and Dragićević, Milan and Simonović, Ana and Subotić, Angelina and Milošević, Snežana and Cingel, Aleksandar",
year = "2021",
abstract = "Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis",
number = "9",
volume = "7",
doi = "10.3390/horticulturae7090324",
pages = "324"
}

Trajković, M., Jevremović, S., Dragićević, M., Simonović, A., Subotić, A., Milošević, S.,& Cingel, A.. (2021). Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis. in Horticulturae
Basel: MDPI., 7(9), 324.
https://doi.org/10.3390/horticulturae7090324

Trajković M, Jevremović S, Dragićević M, Simonović A, Subotić A, Milošević S, Cingel A. Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis. in Horticulturae. 2021;7(9):324.
doi:10.3390/horticulturae7090324 .

Trajković, Milena, Jevremović, Slađana, Dragićević, Milan, Simonović, Ana, Subotić, Angelina, Milošević, Snežana, Cingel, Aleksandar, "Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis" in Horticulturae, 7, no. 9 (2021):324,
https://doi.org/10.3390/horticulturae7090324 . .

TY  - CONF
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Momčilović, Ivana
PY  - 2021
UR  - https://www.vibconferences.be/events/plant-science-for-climate-emergency
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4340
AB  - Potato, Solanum tuberosum L., is the world's most important non-grain food crop, which is highly heat-susceptible. Even moderately elevated temperatures have a significant impact on potato growth and tuberization, limiting productivity. High-temperature stress is accompanied by secondary oxidative stress due to elevation of reactive oxygen species (ROS) levels. Superoxide dismutases (SODs) are metalloenzymes that play an important role of primary ROS scavengers. So far, the SOD gene family was not systematically described in potato, and the data regarding the expression of potato SOD genes under elevated temperature are scarce. We performed an in silico study of potato SODs and analyzed the transcriptional expression in response to moderately elevated (26 °C) and high (29 °C) temperature. The potato genome contains seven SOD genes, including three Cu/ZnSODs, one MnSOD and three FeSODs, unevenly distributed on five chromosomes. Phylogenetic analysis revealed that potato SOD protein sequences share the most similarity to S. lycopersicum SODs and can be separated into two groups: Cu/ZnSOD and FeSOD-MnSOD. Cis-elements related to different signals, including high temperature, plant hormones, and light, were found in promoters of potato SOD genes. Gene expression analysis at different temperature treatments revealed heat-responsive SOD genes. Salicylic acid (SA) is a plant growth regulator and signaling molecule involved in plants' defense responses to abiotic stresses. Application of SA promoted expression of most SODs, especially at moderately elevated (26 °C) temperature. The results of our study revealed heat-inducible SOD genes that might be important for potato antioxidant defense under heat stress.
PB  - VIB Conferences Series
C3  - Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.
T1  - The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression
SP  - 97 / P38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4340
ER  - 

@conference{
author = "Pantelić, Danijel and Rudić, Jelena and Dragićević, Milan and Simonović, Ana and Momčilović, Ivana",
year = "2021",
abstract = "Potato, Solanum tuberosum L., is the world's most important non-grain food crop, which is highly heat-susceptible. Even moderately elevated temperatures have a significant impact on potato growth and tuberization, limiting productivity. High-temperature stress is accompanied by secondary oxidative stress due to elevation of reactive oxygen species (ROS) levels. Superoxide dismutases (SODs) are metalloenzymes that play an important role of primary ROS scavengers. So far, the SOD gene family was not systematically described in potato, and the data regarding the expression of potato SOD genes under elevated temperature are scarce. We performed an in silico study of potato SODs and analyzed the transcriptional expression in response to moderately elevated (26 °C) and high (29 °C) temperature. The potato genome contains seven SOD genes, including three Cu/ZnSODs, one MnSOD and three FeSODs, unevenly distributed on five chromosomes. Phylogenetic analysis revealed that potato SOD protein sequences share the most similarity to S. lycopersicum SODs and can be separated into two groups: Cu/ZnSOD and FeSOD-MnSOD. Cis-elements related to different signals, including high temperature, plant hormones, and light, were found in promoters of potato SOD genes. Gene expression analysis at different temperature treatments revealed heat-responsive SOD genes. Salicylic acid (SA) is a plant growth regulator and signaling molecule involved in plants' defense responses to abiotic stresses. Application of SA promoted expression of most SODs, especially at moderately elevated (26 °C) temperature. The results of our study revealed heat-inducible SOD genes that might be important for potato antioxidant defense under heat stress.",
publisher = "VIB Conferences Series",
journal = "Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.",
title = "The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression",
pages = "97 / P38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4340"
}

Pantelić, D., Rudić, J., Dragićević, M., Simonović, A.,& Momčilović, I.. (2021). The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression. in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.
VIB Conferences Series., 97 / P38.
https://hdl.handle.net/21.15107/rcub_ibiss_4340

Pantelić D, Rudić J, Dragićević M, Simonović A, Momčilović I. The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression. in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.. 2021;:97 / P38.
https://hdl.handle.net/21.15107/rcub_ibiss_4340 .

Pantelić, Danijel, Rudić, Jelena, Dragićević, Milan, Simonović, Ana, Momčilović, Ivana, "The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression" in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97. (2021):97 / P38,
https://hdl.handle.net/21.15107/rcub_ibiss_4340 .