TY  - JOUR
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Subotić, Angelina
AU  - Jerinić-Prodanović, Dušanka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6665
AB  - Abstract
Background Carrot is an important vegetable crop grown worldwide. The major economic problem in carrot cultivation is yellow disease caused by Bactericera trigonica, which induces biotic stress and has the greatest impact on crop productivity. Comprehensive studies on the mechanism of carrot defense response to biotic stress caused by B. trigonica infestation have yet to be conducted.
Methods The changes in photosynthetic pigments, proline, TPC, H2O2 and MDA content, DPPH radical scavenging ability, and antioxidant enzyme activity of SOD, CAT, and POX in carrot leaves in response to insect sex (female and male), rapid response (during the frst six hours), and long-term response to B. trigonica infestation were evaluated.
Results The results of our study strongly suggest that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and oxidative status of carrot leaves. Photosynthetic pigment content, TPC, and DPPH and CAT activities were signifcantly reduced in carrot leaves in response to insect infestation. On the other hand, proline, H2O2 content, and the activity of the antioxidant enzymes superoxide dismutase and peroxidase were increased in carrot leaves after B. trigonica infestation. The results indicate that B. trigonica attenuates and delays the oxidative stress responses of carrot, allowing long-term feeding without visible changes in the plant. Carrot responded to longterm B. trigonica infestation with an increase in SOD and POX activity, suggesting that these enzymes may play a key
role in plant defense mechanisms.
Conclusions This is the frst comprehensive study strongly suggesting that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and an attenuated ROS defense response in carrot leaves that enables long-term insect feeding. The information provides new insights into the mechanisms of carrot protection against B. trigonica infestation.
PB  - Springer Nature
T2  - BMC Plant Biology
T1  - Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation
IS  - 1
VL  - 24
DO  - 10.1186/s12870-024-04946-4
SP  - 243
ER  - 

@article{
author = "Đurić, Marija and Jevremović, Slađana and Trifunović-Momčilov, Milana and Milošević, Snežana and Subotić, Angelina and Jerinić-Prodanović, Dušanka",
year = "2024",
abstract = "Abstract
Background Carrot is an important vegetable crop grown worldwide. The major economic problem in carrot cultivation is yellow disease caused by Bactericera trigonica, which induces biotic stress and has the greatest impact on crop productivity. Comprehensive studies on the mechanism of carrot defense response to biotic stress caused by B. trigonica infestation have yet to be conducted.
Methods The changes in photosynthetic pigments, proline, TPC, H2O2 and MDA content, DPPH radical scavenging ability, and antioxidant enzyme activity of SOD, CAT, and POX in carrot leaves in response to insect sex (female and male), rapid response (during the frst six hours), and long-term response to B. trigonica infestation were evaluated.
Results The results of our study strongly suggest that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and oxidative status of carrot leaves. Photosynthetic pigment content, TPC, and DPPH and CAT activities were signifcantly reduced in carrot leaves in response to insect infestation. On the other hand, proline, H2O2 content, and the activity of the antioxidant enzymes superoxide dismutase and peroxidase were increased in carrot leaves after B. trigonica infestation. The results indicate that B. trigonica attenuates and delays the oxidative stress responses of carrot, allowing long-term feeding without visible changes in the plant. Carrot responded to longterm B. trigonica infestation with an increase in SOD and POX activity, suggesting that these enzymes may play a key
role in plant defense mechanisms.
Conclusions This is the frst comprehensive study strongly suggesting that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and an attenuated ROS defense response in carrot leaves that enables long-term insect feeding. The information provides new insights into the mechanisms of carrot protection against B. trigonica infestation.",
publisher = "Springer Nature",
journal = "BMC Plant Biology",
title = "Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation",
number = "1",
volume = "24",
doi = "10.1186/s12870-024-04946-4",
pages = "243"
}

Đurić, M., Jevremović, S., Trifunović-Momčilov, M., Milošević, S., Subotić, A.,& Jerinić-Prodanović, D.. (2024). Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation. in BMC Plant Biology
Springer Nature., 24(1), 243.
https://doi.org/10.1186/s12870-024-04946-4

Đurić M, Jevremović S, Trifunović-Momčilov M, Milošević S, Subotić A, Jerinić-Prodanović D. Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation. in BMC Plant Biology. 2024;24(1):243.
doi:10.1186/s12870-024-04946-4 .

Đurić, Marija, Jevremović, Slađana, Trifunović-Momčilov, Milana, Milošević, Snežana, Subotić, Angelina, Jerinić-Prodanović, Dušanka, "Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation" in BMC Plant Biology, 24, no. 1 (2024):243,
https://doi.org/10.1186/s12870-024-04946-4 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Jevremović, Slađana
AU  - Uzelac, Branka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6522
AB  - The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime
IS  - 1
VL  - 10
DO  - 10.3390/horticulturae10010017
SP  - 17
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Jevremović, Slađana and Uzelac, Branka",
year = "2024",
abstract = "The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime",
number = "1",
volume = "10",
doi = "10.3390/horticulturae10010017",
pages = "17"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Jevremović, S.,& Uzelac, B.. (2024). Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae
Basel : MDPI., 10(1), 17.
https://doi.org/10.3390/horticulturae10010017

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Jevremović S, Uzelac B. Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae. 2024;10(1):17.
doi:10.3390/horticulturae10010017 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Jevremović, Slađana, Uzelac, Branka, "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime" in Horticulturae, 10, no. 1 (2024):17,
https://doi.org/10.3390/horticulturae10010017 . .

TY  - CONF
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Jevremović, Slađana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6689
AB  - Boja cveta je jedna od najznačajnijih osobina biljaka čija je osnovna uloga u privlačenju oprašivača. Takođe, boja cvetova određuje i komercijalnu vrednost ukrasnih biljaka. Ova osobina biljaka predstavlja jedan od prvih predmeta istraživanja u oblasti primene genetičkog inženjerstva u hortikulturi. Boja cvetova je određena sadržim tri grupe biljnih pigmenata: anticijanina, karotenoida i betalaina. Veliki napredak u istraživanjima je napravljen na modifikaciji boje cvetova genetičkom modifikacijom biosinteze antocijanina. Sadržaj karotenoida u cvetovima (likopen, lutein, β-karoten, anteraksantin, violaksantin) određuje boju cvetova biljaka od žute do crvene. U radu će biti prikazani rezultati na promeni boje cvetova sitnocvetne ljubičice (Viola cornuta L.) kultivara sa žutim cvetovima genetičkom modifikacijom karotenoida. Genetičkom transformacijom, posredstvom Agrobacterium tumefaciens, genom za kapsantin-kapsorubin sintazu poreklom iz cvetova ljiljana (Llccs) pomoću dva vektora (35S-Llccs i PchsA-Llccs) dobijene su biljke sa promenjenom bojom cvetova. Krunični listići cvetova biljaka su promenjeni iz žute u nijanse narandžaste usled akumulacije novosintetisanog pigmenta kapsantina. Pigment se akumulira u hromoplastima ćelija kruničnih listova usled čega je došlo do promene boje cvetova. Na osnovu ovih rezultata zaključeno je da ektopijska ekspresija gena Llccs pod uticajem oba promotora se može koristi za promenu boje cvetova biljaka.
AB  - Боја цветова је једна од најзначајнијих особина биљака чија је основна улога у привлачењу опрашивача. Такође, боја цветова одређује и комерцијалну вредност украсних биљака. Ова особина биљака представља један од првих предмета истраживања у области примене генетичког инжењерства у хортикултури. Боја цветова је одређена садржајем три групе биљних пигмената: антицијанина, каротеноида и беталаина. Велики напредак у истраживањима је направљен на модификацији боје цветова генетичком модификацијом биосинтезе антоцијанина. Садржај каротеноида у цветовима (ликопен, лутеин,  β-каротен, антераксантин, виолаксантин) одређује боју цветова биљака од жуте до црвене. У раду ће бити приказани резултати на промени боје цветова ситноцветне љубичице (Viola cornuta L.) култивара са жутим цветовима генетичком модификацијом каротеноида. Генетичком трансформацијом, посредством Agrobacterium tumefaciens, геном за капсантин-капсорубин синтазу пореклом из цветова љиљана (Llccs) помоћу два вектора (35S-Llccs и PchsA-Llccs) добијене су биљке са промењеном бојом цветова. Крунични листићи цветова биљака су промењени из жуте у нијансе наранџасте услед акумулације новосинтетисаног пигмента капсантина. Пигмент се акумулира у хромопластима ћелија круничних листића услед чега је дошло до промене боје цветова. На основу ових резултата закључено је да ектопијска експресија гена Llccs под утицајем оба промотора се може користити за промену боје цветова биљака.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.)
T1  - Najnovija postignuća na promeni boje cvetova sitnocvetne ljubičice (Viola cornutaL.)
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6689
ER  - 

@conference{
author = "Trajković, Milena and Cingel, Aleksandar and Jevremović, Slađana",
year = "2022",
abstract = "Boja cveta je jedna od najznačajnijih osobina biljaka čija je osnovna uloga u privlačenju oprašivača. Takođe, boja cvetova određuje i komercijalnu vrednost ukrasnih biljaka. Ova osobina biljaka predstavlja jedan od prvih predmeta istraživanja u oblasti primene genetičkog inženjerstva u hortikulturi. Boja cvetova je određena sadržim tri grupe biljnih pigmenata: anticijanina, karotenoida i betalaina. Veliki napredak u istraživanjima je napravljen na modifikaciji boje cvetova genetičkom modifikacijom biosinteze antocijanina. Sadržaj karotenoida u cvetovima (likopen, lutein, β-karoten, anteraksantin, violaksantin) određuje boju cvetova biljaka od žute do crvene. U radu će biti prikazani rezultati na promeni boje cvetova sitnocvetne ljubičice (Viola cornuta L.) kultivara sa žutim cvetovima genetičkom modifikacijom karotenoida. Genetičkom transformacijom, posredstvom Agrobacterium tumefaciens, genom za kapsantin-kapsorubin sintazu poreklom iz cvetova ljiljana (Llccs) pomoću dva vektora (35S-Llccs i PchsA-Llccs) dobijene su biljke sa promenjenom bojom cvetova. Krunični listići cvetova biljaka su promenjeni iz žute u nijanse narandžaste usled akumulacije novosintetisanog pigmenta kapsantina. Pigment se akumulira u hromoplastima ćelija kruničnih listova usled čega je došlo do promene boje cvetova. Na osnovu ovih rezultata zaključeno je da ektopijska ekspresija gena Llccs pod uticajem oba promotora se može koristi za promenu boje cvetova biljaka., Боја цветова је једна од најзначајнијих особина биљака чија је основна улога у привлачењу опрашивача. Такође, боја цветова одређује и комерцијалну вредност украсних биљака. Ова особина биљака представља један од првих предмета истраживања у области примене генетичког инжењерства у хортикултури. Боја цветова је одређена садржајем три групе биљних пигмената: антицијанина, каротеноида и беталаина. Велики напредак у истраживањима је направљен на модификацији боје цветова генетичком модификацијом биосинтезе антоцијанина. Садржај каротеноида у цветовима (ликопен, лутеин,  β-каротен, антераксантин, виолаксантин) одређује боју цветова биљака од жуте до црвене. У раду ће бити приказани резултати на промени боје цветова ситноцветне љубичице (Viola cornuta L.) култивара са жутим цветовима генетичком модификацијом каротеноида. Генетичком трансформацијом, посредством Agrobacterium tumefaciens, геном за капсантин-капсорубин синтазу пореклом из цветова љиљана (Llccs) помоћу два вектора (35S-Llccs и PchsA-Llccs) добијене су биљке са промењеном бојом цветова. Крунични листићи цветова биљака су промењени из жуте у нијансе наранџасте услед акумулације новосинтетисаног пигмента капсантина. Пигмент се акумулира у хромопластима ћелија круничних листића услед чега је дошло до промене боје цветова. На основу ових резултата закључено је да ектопијска експресија гена Llccs под утицајем оба промотора се може користити за промену боје цветова биљака.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.), Najnovija postignuća na promeni boje cvetova sitnocvetne ljubičice (Viola cornutaL.)",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6689"
}

Trajković, M., Cingel, A.,& Jevremović, S.. (2022). Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 70.
https://hdl.handle.net/21.15107/rcub_ibiss_6689

Trajković M, Cingel A, Jevremović S. Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:70.
https://hdl.handle.net/21.15107/rcub_ibiss_6689 .

Trajković, Milena, Cingel, Aleksandar, Jevremović, Slađana, "Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):70,
https://hdl.handle.net/21.15107/rcub_ibiss_6689 .

TY  - CHAP
AU  - Trajković, Milena
AU  - Jevremović, Slađana
AU  - Cingel, Aleksandar
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5302
AB  - U hortikulturi je prisutna stalna potreba za ukrasnim biljkama sa novim karakteristikama, gde boja
cveta predstavlja jednu od najvažnijih osobina koja određuje njihovu komercijalnu vrednost. Sa razvojem
metoda genetičkog inženjeringa otvorena je mogućnost kreiranja biljaka sa željenom bojom cvetova koja
se ne može postići klasičnim ukrštanjem ili mutagenezom. Boja cvetova kod biljaka određena je sadržajem
tri biljna pigmenta: antocijanina, karotenoida i betalaina. Do sada, najveći napredak postignut je genetičkom
modulacijom biosinteze antocijanina. Na ovaj način postignute su nove boje cvetova kod
najmanje 50 ukrasnih vrsta, a neki od tih modifikovanih varijeteta su već dugi niz godina u slobodnoj prodaji.
Međutim, promena boje cveta manipulacijom biosintetskog puta karotenoida je dokumentovana
kod svega nekoliko ukrasnih vrsta i poslednjih godina intenzivirana su istraživanja u tom pravcu. U ovom
radu je razmatran potencijal ovog pristupa, sa posebnim osvrtom na rezultate postignute na promeni boje
cvetova kod kultivara ljubičice uvodjenjem gena za kapsantin-kapsorubin sintazu.
AB  - In horticulture, there is a constant need for ornamental plants with new characteristics, where the flower
color is one of the most important features that determines their commercial value. With the development
of genetic engineering methods, it has been possible to create plants with the desired flower
color which cannot be achieved by classical breeding or mutagenesis. The flower color in plants is determined
by the content of three plant pigments: anthocyanins, carotenoids and betalains. Up to date, the
greatest progress has been made by genetic modulation of anthocyanin biosynthesis. In this way, the new
flower colors have been achieved in at least 50 ornamental species, and some of these modified varieties
have been on market for many years. However, the alteration of flower color by manipulating the carotenoid
biosynthetic pathway has been documented in only a few ornamental species, and the research has
been significantly increased last few years. In this paper, the potential of this approach is considered, with
special reference to the results achieved on flower color alteration of pansy cultivars by introducing the
gene for capsanthin-capsorubin synthase.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering
T2  - Trends in Molecular Biology
T1  - Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida
T1  - Recent advances in flower color alteration by metabolic manipulation of carotenoid biosynthesis
VL  - 2
SP  - 238
EP  - 253
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5302
ER  - 

@inbook{
author = "Trajković, Milena and Jevremović, Slađana and Cingel, Aleksandar",
year = "2022",
abstract = "U hortikulturi je prisutna stalna potreba za ukrasnim biljkama sa novim karakteristikama, gde boja
cveta predstavlja jednu od najvažnijih osobina koja određuje njihovu komercijalnu vrednost. Sa razvojem
metoda genetičkog inženjeringa otvorena je mogućnost kreiranja biljaka sa željenom bojom cvetova koja
se ne može postići klasičnim ukrštanjem ili mutagenezom. Boja cvetova kod biljaka određena je sadržajem
tri biljna pigmenta: antocijanina, karotenoida i betalaina. Do sada, najveći napredak postignut je genetičkom
modulacijom biosinteze antocijanina. Na ovaj način postignute su nove boje cvetova kod
najmanje 50 ukrasnih vrsta, a neki od tih modifikovanih varijeteta su već dugi niz godina u slobodnoj prodaji.
Međutim, promena boje cveta manipulacijom biosintetskog puta karotenoida je dokumentovana
kod svega nekoliko ukrasnih vrsta i poslednjih godina intenzivirana su istraživanja u tom pravcu. U ovom
radu je razmatran potencijal ovog pristupa, sa posebnim osvrtom na rezultate postignute na promeni boje
cvetova kod kultivara ljubičice uvodjenjem gena za kapsantin-kapsorubin sintazu., In horticulture, there is a constant need for ornamental plants with new characteristics, where the flower
color is one of the most important features that determines their commercial value. With the development
of genetic engineering methods, it has been possible to create plants with the desired flower
color which cannot be achieved by classical breeding or mutagenesis. The flower color in plants is determined
by the content of three plant pigments: anthocyanins, carotenoids and betalains. Up to date, the
greatest progress has been made by genetic modulation of anthocyanin biosynthesis. In this way, the new
flower colors have been achieved in at least 50 ornamental species, and some of these modified varieties
have been on market for many years. However, the alteration of flower color by manipulating the carotenoid
biosynthetic pathway has been documented in only a few ornamental species, and the research has
been significantly increased last few years. In this paper, the potential of this approach is considered, with
special reference to the results achieved on flower color alteration of pansy cultivars by introducing the
gene for capsanthin-capsorubin synthase.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering",
journal = "Trends in Molecular Biology",
booktitle = "Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida, Recent advances in flower color alteration by metabolic manipulation of carotenoid biosynthesis",
volume = "2",
pages = "238-253",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5302"
}

Trajković, M., Jevremović, S.,& Cingel, A.. (2022). Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida. in Trends in Molecular Biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering., 2, 238-253.
https://hdl.handle.net/21.15107/rcub_ibiss_5302

Trajković M, Jevremović S, Cingel A. Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida. in Trends in Molecular Biology. 2022;2:238-253.
https://hdl.handle.net/21.15107/rcub_ibiss_5302 .

Trajković, Milena, Jevremović, Slađana, Cingel, Aleksandar, "Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida" in Trends in Molecular Biology, 2 (2022):238-253,
https://hdl.handle.net/21.15107/rcub_ibiss_5302 .

TY  - JOUR
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Trifunović-Momčilov, Milana
AU  - Đurić, Marija
AU  - Koruga, Đuro
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5145
AB  - The rapid production and numerous applications of nanomaterials warrant the necessity
and importance of examining nanoparticles in terms to their environmental and biological effects
and implications. In this study, the effects of a water-soluble hyper-harmonized hydroxyl-modified
fullerene (3HFWC) on cherry tomato seed germination, seedlings growth, physiological response and
fruiting was evaluated. Changes in the photosynthetic pigments content, oxidative stress assessment,
and aquaporin genes expression in cherry tomato plants were studied after during short- and longterm continuous exposure to 3HFWC nanosubstance (200 mg/L). Increased levels of photosynthetic
pigments in leaves, lycopene in fruits, decreased levels of hydrogen peroxide content, activation of
cellular antioxidant enzymes such as superoxide dismutase, catalase and peroxidase and increased
aquaporin gene expression (PIP1;3, PIP1;5 and PIP2;4) were observed in 3HFWC nanosubstanceexposed plants in comparison to control, untreated cherry tomato plants. The 3HFWC nanosubstance
showed positive effects on cherry tomato seed germination, plantlet growth and lycopene content in
fruits and may be considered as a promising nanofertilizer.
PB  - Basel: MDPI
T2  - Plants
T1  - Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex
IS  - 21
VL  - 11
DO  - 10.3390/plants11212810
SP  - 2810
ER  - 

@article{
author = "Subotić, Angelina and Jevremović, Slađana and Milošević, Snežana and Trifunović-Momčilov, Milana and Đurić, Marija and Koruga, Đuro",
year = "2022",
abstract = "The rapid production and numerous applications of nanomaterials warrant the necessity
and importance of examining nanoparticles in terms to their environmental and biological effects
and implications. In this study, the effects of a water-soluble hyper-harmonized hydroxyl-modified
fullerene (3HFWC) on cherry tomato seed germination, seedlings growth, physiological response and
fruiting was evaluated. Changes in the photosynthetic pigments content, oxidative stress assessment,
and aquaporin genes expression in cherry tomato plants were studied after during short- and longterm continuous exposure to 3HFWC nanosubstance (200 mg/L). Increased levels of photosynthetic
pigments in leaves, lycopene in fruits, decreased levels of hydrogen peroxide content, activation of
cellular antioxidant enzymes such as superoxide dismutase, catalase and peroxidase and increased
aquaporin gene expression (PIP1;3, PIP1;5 and PIP2;4) were observed in 3HFWC nanosubstanceexposed plants in comparison to control, untreated cherry tomato plants. The 3HFWC nanosubstance
showed positive effects on cherry tomato seed germination, plantlet growth and lycopene content in
fruits and may be considered as a promising nanofertilizer.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex",
number = "21",
volume = "11",
doi = "10.3390/plants11212810",
pages = "2810"
}

Subotić, A., Jevremović, S., Milošević, S., Trifunović-Momčilov, M., Đurić, M.,& Koruga, Đ.. (2022). Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex. in Plants
Basel: MDPI., 11(21), 2810.
https://doi.org/10.3390/plants11212810

Subotić A, Jevremović S, Milošević S, Trifunović-Momčilov M, Đurić M, Koruga Đ. Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex. in Plants. 2022;11(21):2810.
doi:10.3390/plants11212810 .

Subotić, Angelina, Jevremović, Slađana, Milošević, Snežana, Trifunović-Momčilov, Milana, Đurić, Marija, Koruga, Đuro, "Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex" in Plants, 11, no. 21 (2022):2810,
https://doi.org/10.3390/plants11212810 . .

TY  - JOUR
AU  - Škoro, Nikola
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Puač, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4938
AB  - Herein, we present the effect of surface dielectric barrier discharge (SDBD) air cold plasma on regrowth of chrysanthemum synthetic seeds (synseeds) and subsequent plantlet development. The plasma system used in this study operates in air at the frequency of 50 Hz. The detailed electrical characterization of SDBD was shown, as well as air plasma emission spectra obtained by optical emission spectroscopy. The chrysanthemum synseeds (encapsulated shoot tips) were treated in air plasma for different treatment times (0, 5 or 10 min). Plasma treatment significantly improved the regrowth and whole plantlet development of chrysanthemum synseeds under aseptic (in vitro) and non-aseptic (ex vitro) conditions. We evaluated the effect of SDBD plasma on synseed germination of four chrysanthemum cultivars after direct sowing in soil. Germination of synseeds directly sowed in soil was cultivar-dependent and 1.6−3.7 fold higher after plasma treatment in comparison with untreated synseeds. The study showed a highly effective novel strategy for direct conversion of simple monolayer alginate chrysanthemum synseeds into entire plantlets by plasma pre-conversion treatment. This treatment reduced contamination and displayed a considerable ex vitro ability to convert clonally identical chrysanthemum plants.
PB  - Basel: MDPI
T2  - Plants
T1  - Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma
IS  - 7
VL  - 11
DO  - 10.3390/plants11070907
SP  - 907
ER  - 

@article{
author = "Škoro, Nikola and Živković, Suzana and Jevremović, Slađana and Puač, Nevena",
year = "2022",
abstract = "Herein, we present the effect of surface dielectric barrier discharge (SDBD) air cold plasma on regrowth of chrysanthemum synthetic seeds (synseeds) and subsequent plantlet development. The plasma system used in this study operates in air at the frequency of 50 Hz. The detailed electrical characterization of SDBD was shown, as well as air plasma emission spectra obtained by optical emission spectroscopy. The chrysanthemum synseeds (encapsulated shoot tips) were treated in air plasma for different treatment times (0, 5 or 10 min). Plasma treatment significantly improved the regrowth and whole plantlet development of chrysanthemum synseeds under aseptic (in vitro) and non-aseptic (ex vitro) conditions. We evaluated the effect of SDBD plasma on synseed germination of four chrysanthemum cultivars after direct sowing in soil. Germination of synseeds directly sowed in soil was cultivar-dependent and 1.6−3.7 fold higher after plasma treatment in comparison with untreated synseeds. The study showed a highly effective novel strategy for direct conversion of simple monolayer alginate chrysanthemum synseeds into entire plantlets by plasma pre-conversion treatment. This treatment reduced contamination and displayed a considerable ex vitro ability to convert clonally identical chrysanthemum plants.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma",
number = "7",
volume = "11",
doi = "10.3390/plants11070907",
pages = "907"
}

Škoro, N., Živković, S., Jevremović, S.,& Puač, N.. (2022). Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma. in Plants
Basel: MDPI., 11(7), 907.
https://doi.org/10.3390/plants11070907

Škoro N, Živković S, Jevremović S, Puač N. Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma. in Plants. 2022;11(7):907.
doi:10.3390/plants11070907 .

Škoro, Nikola, Živković, Suzana, Jevremović, Slađana, Puač, Nevena, "Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma" in Plants, 11, no. 7 (2022):907,
https://doi.org/10.3390/plants11070907 . .

TY  - CONF
AU  - Subotić, Angelina
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Đurić, Marija
AU  - Koruga, Đuro
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5395
AB  - Нанотехнологија је релативно нова научна област и неопходна су темељнија 
истраживања да би се помоћу ње могло доћи до корисних сазнања за добробит 
човечанства. Многе земље у свету су свесне потенцијала који пружа примена 
нанотехнологије у пољопривреди и развијају програме који би омогућили 
повећање приноса, заштиту околине од прекомерне употребе хербицида и 
пестицида и производњу функционалне хране. Циљ ових истраживања је био да се 
испита ефекат 200 mg/l 3HWFC+
наносупстанце (патент PCT/EP2019/083307) на 
процес клијања, морфолошке и физиолошке аспекте развића плодова парадајза. У 
фази развића клијанаца успешно је стимулисан пораст биомасе у присуству 
наносупстанце. У овом раду приказан је и позитиван утицај наносупстанце на 
развиће плодова парадајза и њихов квалитет. У погледу квалитета плодова, 
најбољи резултат ефеката наносусптанце огледао се у повећању садржаја ликопена 
код зрелих плодова у односу на плодове контролних биљака. Резултати ових 
истраживања указују на нови научни допринос разумевању ефекта 3HFWC+
наносупстанце на развојне процесе парадајза и такође имају потенцијални значај у 
унапређењу технологије производње ове повртарске културе, односно добијању 
плодова са повећаним садржајем ликопена.
AB  - Nanotehnologija je relativno nova naučna oblast i neophodna su temeljnija istraživanja da bi se pomoću nje moglo doći do korisnih saznanja za dobrobit čovečanstva. Mnoge zemlje u svetu su svesne potencijala koji pruža primena nanotehnologije u poljoprivredi i razvijaju programe koji bi omogućili povećanje prinosa, zaštitu okoline od prekomerne upotrebe herbicida i pesticida i proizvodnju funkcionalne hrane. Cilj ovih istraživanja je bio da se ispita efekat 200 mg/l 3HWFC+ nanosupstance (patent PCT/EP2019/083307) na proces klijanja, morfološke i fiziološke aspekte razvića plodova paradajza. U fazi razvića klijanaca uspešno je stimulisan porast biomase u prisustvu nanosupstance. U ovom radu prikazan je i pozitivan uticaj nanosupstance na razviće plodova paradajza i njihov kvalitet. U pogledu kvaliteta plodova, najbolji rezultat efekata nanosusptance ogledao se u povećanju sadržaja likopena kod zrelih plodova u odnosu na plodove kontrolnih biljaka. Rezultati ovih istraživanja ukazuju na novi naučni doprinos razumevanju efekta 3HFWC+ nanosupstance na razvojne procese paradajza i takođe imaju potencijalni značaj u unapređenju tehnologije proizvodnje ove povrtarske kulture, odnosno dobijanju plodova sa povećanim sadržajem likopena.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.)
T1  - Efekat drugog derivata molekula S60, 3HFWC+ nanosupstance, na morfološke i fiziološke aspekte razvića paradajza (Solanum lycopersicum L.)
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5395
ER  - 

@conference{
author = "Subotić, Angelina and Trifunović-Momčilov, Milana and Milošević, Snežana and Jevremović, Slađana and Đurić, Marija and Koruga, Đuro",
year = "2022",
abstract = "Нанотехнологија је релативно нова научна област и неопходна су темељнија 
истраживања да би се помоћу ње могло доћи до корисних сазнања за добробит 
човечанства. Многе земље у свету су свесне потенцијала који пружа примена 
нанотехнологије у пољопривреди и развијају програме који би омогућили 
повећање приноса, заштиту околине од прекомерне употребе хербицида и 
пестицида и производњу функционалне хране. Циљ ових истраживања је био да се 
испита ефекат 200 mg/l 3HWFC+
наносупстанце (патент PCT/EP2019/083307) на 
процес клијања, морфолошке и физиолошке аспекте развића плодова парадајза. У 
фази развића клијанаца успешно је стимулисан пораст биомасе у присуству 
наносупстанце. У овом раду приказан је и позитиван утицај наносупстанце на 
развиће плодова парадајза и њихов квалитет. У погледу квалитета плодова, 
најбољи резултат ефеката наносусптанце огледао се у повећању садржаја ликопена 
код зрелих плодова у односу на плодове контролних биљака. Резултати ових 
истраживања указују на нови научни допринос разумевању ефекта 3HFWC+
наносупстанце на развојне процесе парадајза и такође имају потенцијални значај у 
унапређењу технологије производње ове повртарске културе, односно добијању 
плодова са повећаним садржајем ликопена., Nanotehnologija je relativno nova naučna oblast i neophodna su temeljnija istraživanja da bi se pomoću nje moglo doći do korisnih saznanja za dobrobit čovečanstva. Mnoge zemlje u svetu su svesne potencijala koji pruža primena nanotehnologije u poljoprivredi i razvijaju programe koji bi omogućili povećanje prinosa, zaštitu okoline od prekomerne upotrebe herbicida i pesticida i proizvodnju funkcionalne hrane. Cilj ovih istraživanja je bio da se ispita efekat 200 mg/l 3HWFC+ nanosupstance (patent PCT/EP2019/083307) na proces klijanja, morfološke i fiziološke aspekte razvića plodova paradajza. U fazi razvića klijanaca uspešno je stimulisan porast biomase u prisustvu nanosupstance. U ovom radu prikazan je i pozitivan uticaj nanosupstance na razviće plodova paradajza i njihov kvalitet. U pogledu kvaliteta plodova, najbolji rezultat efekata nanosusptance ogledao se u povećanju sadržaja likopena kod zrelih plodova u odnosu na plodove kontrolnih biljaka. Rezultati ovih istraživanja ukazuju na novi naučni doprinos razumevanju efekta 3HFWC+ nanosupstance na razvojne procese paradajza i takođe imaju potencijalni značaj u unapređenju tehnologije proizvodnje ove povrtarske kulture, odnosno dobijanju plodova sa povećanim sadržajem likopena.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.), Efekat drugog derivata molekula S60, 3HFWC+ nanosupstance, na morfološke i fiziološke aspekte razvića paradajza (Solanum lycopersicum L.)",
pages = "49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5395"
}

Subotić, A., Trifunović-Momčilov, M., Milošević, S., Jevremović, S., Đurić, M.,& Koruga, Đ.. (2022). Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 49.
https://hdl.handle.net/21.15107/rcub_ibiss_5395

Subotić A, Trifunović-Momčilov M, Milošević S, Jevremović S, Đurić M, Koruga Đ. Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:49.
https://hdl.handle.net/21.15107/rcub_ibiss_5395 .

Subotić, Angelina, Trifunović-Momčilov, Milana, Milošević, Snežana, Jevremović, Slađana, Đurić, Marija, Koruga, Đuro, "Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):49,
https://hdl.handle.net/21.15107/rcub_ibiss_5395 .

TY  - CONF
AU  - Subotić, Angelina
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Jerinić-Prodanović, Dušanka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5396
AB  - Биотички стрес, резултат интеракције између биљке и другог живог организма, 
укључујући и инсекте, има различите негативне ефекте на растење и развиће 
биљака. Циљ овог истраживања је био да се испитају физиолошки и биохемијски аспекти одговора биљака шаргарепе (Daucus carota) на стрес изаван исхраном 
мрквине буве (Bactericera trigonica). Поред директне исхране на биљкама, B.
trigonica је и вектор фастидиозне бактерије Candidatus Liberibacter solanacearum
која проузрокује различите болести код биљака из фамилије Apiaceae. Од 
одгајених имага B. trigonica изоловани су парови од по пет мужјака и женки, а 
потом су наношени на биљке шаргарепе које су претходно гајене у контролисаним условима. Делови листова биљака у експерименту узорковани су након једне и четири недеље, од инокулације инсеката, у циљу одређивања физиолошких и биохемијских одговора на биотички стрес. Код биљака шаргарепе, услед стреса насталог исхраном лисне буве, уочен је измењен садржај фотосинтетичких пигмената. Хистохемијском локализацијом праћена је продукција реактивних форми кисеоника у листовима инокулисаних биљака шаргарепе. Разлике у одговору појединих компоненти антиоксидативног метаболизма изаваних исхраном лисне буве могу бити адаптивна стратегија шаргарепе у одговору на примењени биотички стрес.
AB  - Biotički stres, rezultat interakcije između biljke i drugog živog organizma, uključujući i insekte, ima različite negativne efekte na rastenje i razviće biljaka. Cilj ovog istraživanja je bio da se ispitaju fiziološki i biohemijski aspekti odgovora biljaka šargarepe (Daucus carota) na stres izavan ishranom mrkvine buve (Bactericera trigonica). Pored direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Candidatus Liberibacter solanacearum koja prouzrokuje različite bolesti kod biljaka iz familije Apiaceae. Od odgajenih imaga B. trigonica izolovani su parovi od po pet mužjaka i ženki, a potom su nanošeni na biljke šargarepe koje su prethodno gajene u kontrolisanim uslovima. Delovi listova biljaka u eksperimentu uzorkovani su nakon jedne i četiri nedelje, od inokulacije insekata, u cilju određivanja fizioloških i biohemijskih odgovora na biotički stres. Kod biljaka šargarepe, usled stresa nastalog ishranom lisne buve, uočen je izmenjen sadržaj fotosintetičkih pigmenata. Histohemijskom lokalizacijom praćena je produkcija reaktivnih formi kiseonika u listovima inokulisanih biljaka šargarepe. Razlike u odgovoru pojedinih komponenti antioksidativnog metabolizma izavanih ishranom lisne buve mogu biti adaptivna strategija šargarepe u odgovoru na primenjeni biotički stres.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica
T1  - Fiziološki i biohemijski aspekti odgovora biljaka šargarepe na biotički stres izazvan ishranom mrkvine lisne buve Bactericera trigonica
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5396
ER  - 

@conference{
author = "Subotić, Angelina and Trifunović-Momčilov, Milana and Milošević, Snežana and Đurić, Marija and Jevremović, Slađana and Jerinić-Prodanović, Dušanka",
year = "2022",
abstract = "Биотички стрес, резултат интеракције између биљке и другог живог организма, 
укључујући и инсекте, има различите негативне ефекте на растење и развиће 
биљака. Циљ овог истраживања је био да се испитају физиолошки и биохемијски аспекти одговора биљака шаргарепе (Daucus carota) на стрес изаван исхраном 
мрквине буве (Bactericera trigonica). Поред директне исхране на биљкама, B.
trigonica је и вектор фастидиозне бактерије Candidatus Liberibacter solanacearum
која проузрокује различите болести код биљака из фамилије Apiaceae. Од 
одгајених имага B. trigonica изоловани су парови од по пет мужјака и женки, а 
потом су наношени на биљке шаргарепе које су претходно гајене у контролисаним условима. Делови листова биљака у експерименту узорковани су након једне и четири недеље, од инокулације инсеката, у циљу одређивања физиолошких и биохемијских одговора на биотички стрес. Код биљака шаргарепе, услед стреса насталог исхраном лисне буве, уочен је измењен садржај фотосинтетичких пигмената. Хистохемијском локализацијом праћена је продукција реактивних форми кисеоника у листовима инокулисаних биљака шаргарепе. Разлике у одговору појединих компоненти антиоксидативног метаболизма изаваних исхраном лисне буве могу бити адаптивна стратегија шаргарепе у одговору на примењени биотички стрес., Biotički stres, rezultat interakcije između biljke i drugog živog organizma, uključujući i insekte, ima različite negativne efekte na rastenje i razviće biljaka. Cilj ovog istraživanja je bio da se ispitaju fiziološki i biohemijski aspekti odgovora biljaka šargarepe (Daucus carota) na stres izavan ishranom mrkvine buve (Bactericera trigonica). Pored direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Candidatus Liberibacter solanacearum koja prouzrokuje različite bolesti kod biljaka iz familije Apiaceae. Od odgajenih imaga B. trigonica izolovani su parovi od po pet mužjaka i ženki, a potom su nanošeni na biljke šargarepe koje su prethodno gajene u kontrolisanim uslovima. Delovi listova biljaka u eksperimentu uzorkovani su nakon jedne i četiri nedelje, od inokulacije insekata, u cilju određivanja fizioloških i biohemijskih odgovora na biotički stres. Kod biljaka šargarepe, usled stresa nastalog ishranom lisne buve, uočen je izmenjen sadržaj fotosintetičkih pigmenata. Histohemijskom lokalizacijom praćena je produkcija reaktivnih formi kiseonika u listovima inokulisanih biljaka šargarepe. Razlike u odgovoru pojedinih komponenti antioksidativnog metabolizma izavanih ishranom lisne buve mogu biti adaptivna strategija šargarepe u odgovoru na primenjeni biotički stres.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica, Fiziološki i biohemijski aspekti odgovora biljaka šargarepe na biotički stres izazvan ishranom mrkvine lisne buve Bactericera trigonica",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5396"
}

Subotić, A., Trifunović-Momčilov, M., Milošević, S., Đurić, M., Jevremović, S.,& Jerinić-Prodanović, D.. (2022). Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 48.
https://hdl.handle.net/21.15107/rcub_ibiss_5396

Subotić A, Trifunović-Momčilov M, Milošević S, Đurić M, Jevremović S, Jerinić-Prodanović D. Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:48.
https://hdl.handle.net/21.15107/rcub_ibiss_5396 .

Subotić, Angelina, Trifunović-Momčilov, Milana, Milošević, Snežana, Đurić, Marija, Jevremović, Slađana, Jerinić-Prodanović, Dušanka, "Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):48,
https://hdl.handle.net/21.15107/rcub_ibiss_5396 .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Petrović, Zoran Lj.
AU  - Škoro, Nikola
AU  - Puač, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5268
AB  - The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.
PB  - Bratislava: FMFI UK
C3  - Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
T1  - Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5268
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Gašić, Uroš and Milutinović, Milica and Petrović, Zoran Lj. and Škoro, Nikola and Puač, Nevena",
year = "2022",
abstract = "The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.",
publisher = "Bratislava: FMFI UK",
journal = "Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia",
title = "Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment",
pages = "137",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5268"
}

Živković, S., Jevremović, S., Gašić, U., Milutinović, M., Petrović, Z. Lj., Škoro, N.,& Puač, N.. (2022). Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
Bratislava: FMFI UK., 137.
https://hdl.handle.net/21.15107/rcub_ibiss_5268

Živković S, Jevremović S, Gašić U, Milutinović M, Petrović ZL, Škoro N, Puač N. Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. 2022;:137.
https://hdl.handle.net/21.15107/rcub_ibiss_5268 .

Živković, Suzana, Jevremović, Slađana, Gašić, Uroš, Milutinović, Milica, Petrović, Zoran Lj., Škoro, Nikola, Puač, Nevena, "Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment" in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia (2022):137,
https://hdl.handle.net/21.15107/rcub_ibiss_5268 .

TY  - CONF
AU  - Jevremović, Slađana
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5153
AB  - Идеја о вештачким семенима датира још од 1977. године када је Murashige1 предложио да вештачка семена представљају „инкапсулиране соматске ембрионе“. Данас се под термином вештачка (синтетичка, артифицијална) семена предразумева било који инкапсулирани биљни материјал (соматски ембриони, врхови апикалних или аксиларних изданака, ћелијски агрегати) који се може користити за гајење, чување, пропагацију и добијање комплетних биљака. Основа технологије вештачких семена је имитирање услова за нормално растење и развиће биљака као што се дешава током клијања и формирања биљака из семена. Технологија вештачких семена је искоришћена и као део појединих протокола за краткотрајно чување биљног материјала методама успореног растења као и за његово дуготрајно чување методама криопрезервације. Ова технологија је од великог значаја за биљне врсте које производе невијабилна семена, семена која тешко клијају, формирају мали број семена или их уопште не производе. Неке од таквих биљних врста су и заштићене и угрожене врсте које најчешће показују проблеме у развићу семена и репродукцији. Такође, ова технологија се показала као погодна за пропагацију и чување различитих дрвенастих, лековитих или украсних биљака које се вегетативно умножавају. У раду ће бити приказана методологија добијања вештачких семена као и досадашњи резултати на примени технологије пропагације врхова изданака хризантема и ћелијских суспензија кестена.
AB  - Ideja o veštačkim semenima datira još od 1977. godine kada je Murashige1 predložio da veštačka semena predstavljaju „inkapsulirane somatske embrione“. Danas se pod terminom veštačka (sintetička, artificijalna) semena predrazumeva bilo koji inkapsulirani biljni materijal (somatski embrioni, vrhovi apikalnih ili aksilarnih izdanaka, ćelijski agregati) koji se može koristiti za gajenje, čuvanje, propagaciju i dobijanje kompletnih biljaka. Osnova tehnologije veštačkih semena je imitiranje uslova za normalno rastenje i razviće biljaka kao što se dešava tokom klijanja i formiranja biljaka iz semena. Tehnologija veštačkih semena je iskorišćena i kao deo pojedinih protokola za kratkotrajno čuvanje biljnog materijala metodama usporenog rastenja kao i za njegovo dugotrajno čuvanje metodama krioprezervacije. Ova tehnologija je od velikog značaja za biljne vrste koje proizvode nevijabilna semena, semena koja teško klijaju, formiraju mali broj semena ili ih uopšte ne proizvode. Neke od takvih biljnih vrsta su i zaštićene i ugrožene vrste koje najčešće pokazuju probleme u razviću semena i reprodukciji. Takođe, ova tehnologija se pokazala kao pogodna za propagaciju i čuvanje različitih drvenastih, lekovitih ili ukrasnih biljaka koje se vegetativno umnožavaju. U radu će biti prikazana metodologija dobijanja veštačkih semena kao i dosadašnji rezultati na primeni tehnologije propagacije vrhova izdanaka hrizantema i ćelijskih suspenzija kestena.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Примена технологије вештачких семена у пропагацији и  конзервацији биљака
T1  - Primena tehnologije veštačkih semena u propagaciji i konzervaciji biljaka
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5153
ER  - 

@conference{
author = "Jevremović, Slađana and Ćalić, Dušica and Zdravković-Korać, Snežana",
year = "2022",
abstract = "Идеја о вештачким семенима датира још од 1977. године када је Murashige1 предложио да вештачка семена представљају „инкапсулиране соматске ембрионе“. Данас се под термином вештачка (синтетичка, артифицијална) семена предразумева било који инкапсулирани биљни материјал (соматски ембриони, врхови апикалних или аксиларних изданака, ћелијски агрегати) који се може користити за гајење, чување, пропагацију и добијање комплетних биљака. Основа технологије вештачких семена је имитирање услова за нормално растење и развиће биљака као што се дешава током клијања и формирања биљака из семена. Технологија вештачких семена је искоришћена и као део појединих протокола за краткотрајно чување биљног материјала методама успореног растења као и за његово дуготрајно чување методама криопрезервације. Ова технологија је од великог значаја за биљне врсте које производе невијабилна семена, семена која тешко клијају, формирају мали број семена или их уопште не производе. Неке од таквих биљних врста су и заштићене и угрожене врсте које најчешће показују проблеме у развићу семена и репродукцији. Такође, ова технологија се показала као погодна за пропагацију и чување различитих дрвенастих, лековитих или украсних биљака које се вегетативно умножавају. У раду ће бити приказана методологија добијања вештачких семена као и досадашњи резултати на примени технологије пропагације врхова изданака хризантема и ћелијских суспензија кестена., Ideja o veštačkim semenima datira još od 1977. godine kada je Murashige1 predložio da veštačka semena predstavljaju „inkapsulirane somatske embrione“. Danas se pod terminom veštačka (sintetička, artificijalna) semena predrazumeva bilo koji inkapsulirani biljni materijal (somatski embrioni, vrhovi apikalnih ili aksilarnih izdanaka, ćelijski agregati) koji se može koristiti za gajenje, čuvanje, propagaciju i dobijanje kompletnih biljaka. Osnova tehnologije veštačkih semena je imitiranje uslova za normalno rastenje i razviće biljaka kao što se dešava tokom klijanja i formiranja biljaka iz semena. Tehnologija veštačkih semena je iskorišćena i kao deo pojedinih protokola za kratkotrajno čuvanje biljnog materijala metodama usporenog rastenja kao i za njegovo dugotrajno čuvanje metodama krioprezervacije. Ova tehnologija je od velikog značaja za biljne vrste koje proizvode nevijabilna semena, semena koja teško klijaju, formiraju mali broj semena ili ih uopšte ne proizvode. Neke od takvih biljnih vrsta su i zaštićene i ugrožene vrste koje najčešće pokazuju probleme u razviću semena i reprodukciji. Takođe, ova tehnologija se pokazala kao pogodna za propagaciju i čuvanje različitih drvenastih, lekovitih ili ukrasnih biljaka koje se vegetativno umnožavaju. U radu će biti prikazana metodologija dobijanja veštačkih semena kao i dosadašnji rezultati na primeni tehnologije propagacije vrhova izdanaka hrizantema i ćelijskih suspenzija kestena.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Примена технологије вештачких семена у пропагацији и  конзервацији биљака, Primena tehnologije veštačkih semena u propagaciji i konzervaciji biljaka",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5153"
}

Jevremović, S., Ćalić, D.,& Zdravković-Korać, S.. (2022). Примена технологије вештачких семена у пропагацији и  конзервацији биљака. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5153

Jevremović S, Ćalić D, Zdravković-Korać S. Примена технологије вештачких семена у пропагацији и  конзервацији биљака. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5153 .

Jevremović, Slađana, Ćalić, Dušica, Zdravković-Korać, Snežana, "Примена технологије вештачких семена у пропагацији и  конзервацији биљака" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5153 .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2021
UR  - https://doi.org/10.3390/plants10050902
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4228
AB  - In ornamental geophytes, conventional vegetative propagation is not economically feasible due to very slow development and ineffective methods. It can take several years until a new plant is formed and commercial profitability is achieved. Therefore, micropropagation techniques have been developed to increase the multiplication rate and thus shorten the multiplication and regeneration period. The majority of these techniques rely on the formation of new bulbs and their sprouting. Dormancy is one of the main limiting factors to speed up multiplication in vitro. Bulbous species have a period of bulb dormancy which enables them to survive unfavorable natural conditions. Bulbs grown in vitro also exhibit dormancy, which has to be overcome in order to allow sprouting of bulbs in the next vegetation period. During the period of dormancy, numerous physiological processes occur, many of which have not been elucidated yet. Understanding the process of dormancy will allow us to speed up and improve breeding of geophytes and thereby achieve economic profitability, which is very important for horticulture. This review focuses on recent findings in the area of bulb dormancy initiation and release in fritillaries, with particular emphasis on the effect of plant growth regulators and low-temperature pretreatment on dormancy release in relation to induction of antioxidative enzymes’ activity in vitro.
PB  - MDPI AG
T2  - Plants
T1  - Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters
IS  - 5
VL  - 10
DO  - 10.3390/plants10050902
SP  - 902
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Jevremović, Slađana and Subotić, Angelina",
year = "2021",
abstract = "In ornamental geophytes, conventional vegetative propagation is not economically feasible due to very slow development and ineffective methods. It can take several years until a new plant is formed and commercial profitability is achieved. Therefore, micropropagation techniques have been developed to increase the multiplication rate and thus shorten the multiplication and regeneration period. The majority of these techniques rely on the formation of new bulbs and their sprouting. Dormancy is one of the main limiting factors to speed up multiplication in vitro. Bulbous species have a period of bulb dormancy which enables them to survive unfavorable natural conditions. Bulbs grown in vitro also exhibit dormancy, which has to be overcome in order to allow sprouting of bulbs in the next vegetation period. During the period of dormancy, numerous physiological processes occur, many of which have not been elucidated yet. Understanding the process of dormancy will allow us to speed up and improve breeding of geophytes and thereby achieve economic profitability, which is very important for horticulture. This review focuses on recent findings in the area of bulb dormancy initiation and release in fritillaries, with particular emphasis on the effect of plant growth regulators and low-temperature pretreatment on dormancy release in relation to induction of antioxidative enzymes’ activity in vitro.",
publisher = "MDPI AG",
journal = "Plants",
title = "Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters",
number = "5",
volume = "10",
doi = "10.3390/plants10050902",
pages = "902"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Jevremović, S.,& Subotić, A.. (2021). Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters. in Plants
MDPI AG., 10(5), 902.
https://doi.org/10.3390/plants10050902

Marković M, Trifunović-Momčilov M, Uzelac B, Jevremović S, Subotić A. Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters. in Plants. 2021;10(5):902.
doi:10.3390/plants10050902 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Jevremović, Slađana, Subotić, Angelina, "Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters" in Plants, 10, no. 5 (2021):902,
https://doi.org/10.3390/plants10050902 . .

TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Jevremović, Slađana
AU  - Petrović, Zoran Lj.
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5415
AB  - In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm  The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements  The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment  We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE)  Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins
PB  - Greifswald: Leibniz Institute for Plasma Science and Technology
C3  - Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany
T1  - Changing the plant tissue morphology and physiology by plasma treatment
SP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5415
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Živković, Suzana and Milutinović, Milica and Jevremović, Slađana and Petrović, Zoran Lj.",
year = "2021",
abstract = "In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm  The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements  The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment  We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE)  Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins",
publisher = "Greifswald: Leibniz Institute for Plasma Science and Technology",
journal = "Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany",
title = "Changing the plant tissue morphology and physiology by plasma treatment",
pages = "14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5415"
}

Puač, N., Škoro, N., Živković, S., Milutinović, M., Jevremović, S.,& Petrović, Z. Lj.. (2021). Changing the plant tissue morphology and physiology by plasma treatment. in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany
Greifswald: Leibniz Institute for Plasma Science and Technology., 14.
https://hdl.handle.net/21.15107/rcub_ibiss_5415

Puač N, Škoro N, Živković S, Milutinović M, Jevremović S, Petrović ZL. Changing the plant tissue morphology and physiology by plasma treatment. in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany. 2021;:14.
https://hdl.handle.net/21.15107/rcub_ibiss_5415 .

Puač, Nevena, Škoro, Nikola, Živković, Suzana, Milutinović, Milica, Jevremović, Slađana, Petrović, Zoran Lj., "Changing the plant tissue morphology and physiology by plasma treatment" in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany (2021):14,
https://hdl.handle.net/21.15107/rcub_ibiss_5415 .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Petrović, Zoran Lj
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4414
AB  - Expansion of the plasma agriculture and plasma medicine and the demand for precise and localized in vivo treatments of living cells and tissues resulted in fast development of various plasma devices that operate at atmospheric pressure [1,2]. Irises can be regenerated in vitro by process of somatic embryogenesis and/or organogenesis by formation of shoot or root meristems on calli. During the induction of regeneration process, three types of calli could be distinguished, two friable regenerative calli: white embryogenic and green organogenic and the most abundant yellow, compact, nodular type of non-regenerative calli, designed as non embryonic [3]. Due to its lack of morphogenetic response and/or their low regeneration potential, the regeneration of non-embryogenic iris calli is one of the greatest challenges in this field of investigation. In the current study plant undifferentiated compact tissue (calli) of Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma needle device operating with He as a working gas and changes at morphological and biochemical level were investigated. The plasma needle was positioned 3mm above the callus surface enabling direct contact between the active plasma volume and the surface. Direct plasma treatment triggered significant morphological alterations in structure of non-embryonic calli. Observed changes could be attributed to the enhanced cell division of the plant cells at the surface of the compact calli and differentiation of friable calli type stimulated by reactive species formed in the low temperature plasma. Indicated morphological changes were followed by the significant alteration in secondary metabolites in derived different calli types. Our results implicate that direct plasma treatment could serve as a significant elicitor of secondary metabolites production in dwarf bearded iris calli.
PB  - National Institute for Lasers, Plasma and Radiation Physics
PB  - Alexandru Ioan Cuza University
PB  - Romanian Physical Society
C3  - 19th International conference on plasma physics and applications, CPPA 2021
T1  - Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli
SP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4414
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Gašić, Uroš and Milutinović, Milica and Puač, Nevena and Škoro, Nikola and Petrović, Zoran Lj",
year = "2021",
abstract = "Expansion of the plasma agriculture and plasma medicine and the demand for precise and localized in vivo treatments of living cells and tissues resulted in fast development of various plasma devices that operate at atmospheric pressure [1,2]. Irises can be regenerated in vitro by process of somatic embryogenesis and/or organogenesis by formation of shoot or root meristems on calli. During the induction of regeneration process, three types of calli could be distinguished, two friable regenerative calli: white embryogenic and green organogenic and the most abundant yellow, compact, nodular type of non-regenerative calli, designed as non embryonic [3]. Due to its lack of morphogenetic response and/or their low regeneration potential, the regeneration of non-embryogenic iris calli is one of the greatest challenges in this field of investigation. In the current study plant undifferentiated compact tissue (calli) of Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma needle device operating with He as a working gas and changes at morphological and biochemical level were investigated. The plasma needle was positioned 3mm above the callus surface enabling direct contact between the active plasma volume and the surface. Direct plasma treatment triggered significant morphological alterations in structure of non-embryonic calli. Observed changes could be attributed to the enhanced cell division of the plant cells at the surface of the compact calli and differentiation of friable calli type stimulated by reactive species formed in the low temperature plasma. Indicated morphological changes were followed by the significant alteration in secondary metabolites in derived different calli types. Our results implicate that direct plasma treatment could serve as a significant elicitor of secondary metabolites production in dwarf bearded iris calli.",
publisher = "National Institute for Lasers, Plasma and Radiation Physics, Alexandru Ioan Cuza University, Romanian Physical Society",
journal = "19th International conference on plasma physics and applications, CPPA 2021",
title = "Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4414"
}

Živković, S., Jevremović, S., Gašić, U., Milutinović, M., Puač, N., Škoro, N.,& Petrović, Z. L.. (2021). Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli. in 19th International conference on plasma physics and applications, CPPA 2021
National Institute for Lasers, Plasma and Radiation Physics., 130.
https://hdl.handle.net/21.15107/rcub_ibiss_4414

Živković S, Jevremović S, Gašić U, Milutinović M, Puač N, Škoro N, Petrović ZL. Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli. in 19th International conference on plasma physics and applications, CPPA 2021. 2021;:130.
https://hdl.handle.net/21.15107/rcub_ibiss_4414 .

Živković, Suzana, Jevremović, Slađana, Gašić, Uroš, Milutinović, Milica, Puač, Nevena, Škoro, Nikola, Petrović, Zoran Lj, "Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli" in 19th International conference on plasma physics and applications, CPPA 2021 (2021):130,
https://hdl.handle.net/21.15107/rcub_ibiss_4414 .

TY  - JOUR
AU  - Filipović, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Simonović, Ana
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Subotić, Angelina
PY  - 2021
UR  - https://link.springer.com/article/10.1007/s11627-020-10143-3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4231
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4473
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.
PB  - Springer
T2  - In Vitro Cellular and Developmental Biology - Plant
T1  - Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)
IS  - 3
VL  - 57
DO  - 10.1007/s11627-020-10143-3
SP  - 470
EP  - 480
ER  - 

@article{
author = "Filipović, Biljana and Trifunović-Momčilov, Milana and Simonović, Ana and Jevremović, Slađana and Milošević, Snežana and Subotić, Angelina",
year = "2021",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.",
publisher = "Springer",
journal = "In Vitro Cellular and Developmental Biology - Plant",
title = "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)",
number = "3",
volume = "57",
doi = "10.1007/s11627-020-10143-3",
pages = "470-480"
}

Filipović, B., Trifunović-Momčilov, M., Simonović, A., Jevremović, S., Milošević, S.,& Subotić, A.. (2021). Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant
Springer., 57(3), 470-480.
https://doi.org/10.1007/s11627-020-10143-3

Filipović B, Trifunović-Momčilov M, Simonović A, Jevremović S, Milošević S, Subotić A. Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant. 2021;57(3):470-480.
doi:10.1007/s11627-020-10143-3 .

Filipović, Biljana, Trifunović-Momčilov, Milana, Simonović, Ana, Jevremović, Slađana, Milošević, Snežana, Subotić, Angelina, "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)" in In Vitro Cellular and Developmental Biology - Plant, 57, no. 3 (2021):470-480,
https://doi.org/10.1007/s11627-020-10143-3 . .

TY  - JOUR
AU  - Filipović, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Simonović, Ana
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Subotić, Angelina
PY  - 2021
UR  - https://link.springer.com/article/10.1007/s11627-020-10143-3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4231
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.
PB  - Springer
T2  - In Vitro Cellular and Developmental Biology - Plant
T1  - Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)
IS  - 3
VL  - 57
DO  - 10.1007/s11627-020-10143-3
SP  - 470
EP  - 480
ER  - 

@article{
author = "Filipović, Biljana and Trifunović-Momčilov, Milana and Simonović, Ana and Jevremović, Slađana and Milošević, Snežana and Subotić, Angelina",
year = "2021",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.",
publisher = "Springer",
journal = "In Vitro Cellular and Developmental Biology - Plant",
title = "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)",
number = "3",
volume = "57",
doi = "10.1007/s11627-020-10143-3",
pages = "470-480"
}

Filipović, B., Trifunović-Momčilov, M., Simonović, A., Jevremović, S., Milošević, S.,& Subotić, A.. (2021). Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant
Springer., 57(3), 470-480.
https://doi.org/10.1007/s11627-020-10143-3

Filipović B, Trifunović-Momčilov M, Simonović A, Jevremović S, Milošević S, Subotić A. Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant. 2021;57(3):470-480.
doi:10.1007/s11627-020-10143-3 .

Filipović, Biljana, Trifunović-Momčilov, Milana, Simonović, Ana, Jevremović, Slađana, Milošević, Snežana, Subotić, Angelina, "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)" in In Vitro Cellular and Developmental Biology - Plant, 57, no. 3 (2021):470-480,
https://doi.org/10.1007/s11627-020-10143-3 . .

TY  - JOUR
AU  - Trajković, Milena
AU  - Jevremović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Subotić, Angelina
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4434
AB  - Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis
IS  - 9
VL  - 7
DO  - 10.3390/horticulturae7090324
SP  - 324
ER  - 

@article{
author = "Trajković, Milena and Jevremović, Slađana and Dragićević, Milan and Simonović, Ana and Subotić, Angelina and Milošević, Snežana and Cingel, Aleksandar",
year = "2021",
abstract = "Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis",
number = "9",
volume = "7",
doi = "10.3390/horticulturae7090324",
pages = "324"
}

Trajković, M., Jevremović, S., Dragićević, M., Simonović, A., Subotić, A., Milošević, S.,& Cingel, A.. (2021). Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis. in Horticulturae
Basel: MDPI., 7(9), 324.
https://doi.org/10.3390/horticulturae7090324

Trajković M, Jevremović S, Dragićević M, Simonović A, Subotić A, Milošević S, Cingel A. Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis. in Horticulturae. 2021;7(9):324.
doi:10.3390/horticulturae7090324 .

Trajković, Milena, Jevremović, Slađana, Dragićević, Milan, Simonović, Ana, Subotić, Angelina, Milošević, Snežana, Cingel, Aleksandar, "Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis" in Horticulturae, 7, no. 9 (2021):324,
https://doi.org/10.3390/horticulturae7090324 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana Č.
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4501
AB  - The effects of graded sodium chloride (NaCl) concentrations (0-, 50-, 100-, 150-, and 200-
mM) on photosynthetic pigment contents in in vitro grown shoots of important medicinal plant
species (Centaurium erythraea Rafn) were investigated. Non-transformed, one AtCKX1 and two
AtCKX2 transgenic centaury lines, with altered cytokinin profiles, were used in this study. The chlorophyll
(Chl) and carotenoid contents differed in the non-transformed and transgenic lines. In general,
salinity significantly reduced the Chl a and Chl b contents in comparison to the NaCl-free medium.
The lowest Chl content was observed in AtCKX2 transgenic shoots grown on all the culture
media. The total carotenoid content was increased in shoots of non-transformed and both AtCKX2
transgenic lines grown in 50-mM NaCl. On the other hand, in concentrations ˃50-mM NaCl, the
total carotenoid content was decreased in all analysed centaury shoots. The Chl a/Chl b ratio in all
the shoots increased progressively in the graded NaCl concentrations. Contrarily, the addition of
NaCl in the culture medium reduced the Chl/carotenoid ratio in centaury shoots. Taken together,
the results of this study partly explained the mode of centaury plant adaptations to salt stress in
vitro. Thus, the results on centaury shoots confirmed that the determination of the photosynthetic
pigment contents can be a very useful non-destructive screening method in order to discriminate
susceptible and resistant plant species/lines to salt stress conditions.
PB  - Basel: MDPI
T2  - Agronomy
T1  - Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro
IS  - 10
VL  - 11
DO  - 10.3390/agronomy11102056
SP  - 2056
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Đurić, Marija and Jevremović, Slađana and Dragićević, Ivana Č. and Subotić, Angelina",
year = "2021",
abstract = "The effects of graded sodium chloride (NaCl) concentrations (0-, 50-, 100-, 150-, and 200-
mM) on photosynthetic pigment contents in in vitro grown shoots of important medicinal plant
species (Centaurium erythraea Rafn) were investigated. Non-transformed, one AtCKX1 and two
AtCKX2 transgenic centaury lines, with altered cytokinin profiles, were used in this study. The chlorophyll
(Chl) and carotenoid contents differed in the non-transformed and transgenic lines. In general,
salinity significantly reduced the Chl a and Chl b contents in comparison to the NaCl-free medium.
The lowest Chl content was observed in AtCKX2 transgenic shoots grown on all the culture
media. The total carotenoid content was increased in shoots of non-transformed and both AtCKX2
transgenic lines grown in 50-mM NaCl. On the other hand, in concentrations ˃50-mM NaCl, the
total carotenoid content was decreased in all analysed centaury shoots. The Chl a/Chl b ratio in all
the shoots increased progressively in the graded NaCl concentrations. Contrarily, the addition of
NaCl in the culture medium reduced the Chl/carotenoid ratio in centaury shoots. Taken together,
the results of this study partly explained the mode of centaury plant adaptations to salt stress in
vitro. Thus, the results on centaury shoots confirmed that the determination of the photosynthetic
pigment contents can be a very useful non-destructive screening method in order to discriminate
susceptible and resistant plant species/lines to salt stress conditions.",
publisher = "Basel: MDPI",
journal = "Agronomy",
title = "Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro",
number = "10",
volume = "11",
doi = "10.3390/agronomy11102056",
pages = "2056"
}

Trifunović-Momčilov, M., Milošević, S., Marković, M., Đurić, M., Jevremović, S., Dragićević, I. Č.,& Subotić, A.. (2021). Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro. in Agronomy
Basel: MDPI., 11(10), 2056.
https://doi.org/10.3390/agronomy11102056

Trifunović-Momčilov M, Milošević S, Marković M, Đurić M, Jevremović S, Dragićević IČ, Subotić A. Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro. in Agronomy. 2021;11(10):2056.
doi:10.3390/agronomy11102056 .

Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Đurić, Marija, Jevremović, Slađana, Dragićević, Ivana Č., Subotić, Angelina, "Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro" in Agronomy, 11, no. 10 (2021):2056,
https://doi.org/10.3390/agronomy11102056 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Václav
AU  - Dobrev, Petre I.
AU  - Marković, Marija
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana Č.
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4649
AB  - Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.
PB  - Springer Nature
T2  - Scientific Reports
T1  - Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro
VL  - 11
DO  - 10.1038/s41598-021-00866-7
SP  - 21471
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Motyka, Václav and Dobrev, Petre I. and Marković, Marija and Milošević, Snežana and Jevremović, Slađana and Dragićević, Ivana Č. and Subotić, Angelina",
year = "2021",
abstract = "Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro",
volume = "11",
doi = "10.1038/s41598-021-00866-7",
pages = "21471"
}

Trifunović-Momčilov, M., Motyka, V., Dobrev, P. I., Marković, M., Milošević, S., Jevremović, S., Dragićević, I. Č.,& Subotić, A.. (2021). Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro. in Scientific Reports
Springer Nature., 11, 21471.
https://doi.org/10.1038/s41598-021-00866-7

Trifunović-Momčilov M, Motyka V, Dobrev PI, Marković M, Milošević S, Jevremović S, Dragićević IČ, Subotić A. Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro. in Scientific Reports. 2021;11:21471.
doi:10.1038/s41598-021-00866-7 .

Trifunović-Momčilov, Milana, Motyka, Václav, Dobrev, Petre I., Marković, Marija, Milošević, Snežana, Jevremović, Slađana, Dragićević, Ivana Č., Subotić, Angelina, "Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro" in Scientific Reports, 11 (2021):21471,
https://doi.org/10.1038/s41598-021-00866-7 . .

TY  - CONF
AU  - Jerinić-Prodanović, Dušanka
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4905
AB  - Bactericera trigonica je jedna od malobrojnih štetočina koje se javljaju na gajenim
vrstama iz familije Apiaceae. Godišnje može da razvije i do devet generacija, a prisustvo svih
stadijuma razvića na gajenim biljkama je potvrđeno tokom cele vegetacije. Ishranom imaga i
larve ne dovode do vidljivih simptoma oštećenja na napadnutim biljkama. Ukoliko se jave u
povećanoj brojnosti mogu prouzrokovati sušenje pojedinih delova ili čak celih biljaka. Pored
direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Ca. Liberibacter
solanacearum sa zaraženih na zdrave biljke šargarepe i celera. U prirodnim uslovima, kao i pri
uzgoju na poljoprivrednim površinama, biljke su tokom svog životnog ciklusa izložene
promjenjivim uslovima životne sredine koji mogu na različite načine delovati na njihovo
razviće i rastenje. Biotski faktori, poput napada insekata izazivaju promene na svim nivoima
organizacije biljke, od anatomskih i morfoloških osobina do ćelijskog, biohemijskog i
molekularnog nivoa. U eksperiment su bile uključene biljke šargarepe (Daucus carota),
peršuna (Pastinaca sativa) i celera (Apium graveolens), koje smo gajili u saksijama u fitotronu
u kontrolisanim uslovima. U svakoj saksiji se nalazilo po tri biljke. Imaga B. trigonica smo
sakupljali u usevu šargarepe u Futogu, a zatim smo odgajili jednu generaciju na šargarepi u
laboratoriji pri sobnoj temperaturi. Od odgajenih imaga B. trigonica smo formnirali parove po
tri mužjaka i tri ženke, koje smo nanosili na predhodno izolovane biljke sa cilidrima od
markizeta. Ovako izolovane biljke sa nanetim lisnim buvama smo gajili na sobnoj temperaturi.
Radi analize fizioloških i biohemijskih odgovora biljaka, nastalih ishranom imaga i larvi lisne
buve, uzimani su uzorci listova sa svih biljaka. Prvo uzorkovanje je bilo nakon nanošenja
imaga lisne buve, a drugo kada su na biljkama masovno bile larve. Kod biljaka su praćeni
fiziološki parametri (sadržaj fotosintetičkih pigmenata, hlorofila i karotenoida) i biohemijski
parametri (stepen lipidne peroksidacije (MDA), koncetracija vodonik-peroksida (H2O2),
aktivnost oksidativnih enzima, peroksidaza (POX), katalaza (CAT) i super-okisid dismutaza
(SOD)), kao odgovori biljaka na stres indukovan ishranom imaga i larvi lisne buve. Usled
povreda nastalih ishranom lisne buve kod svih eksperimentalnih biljaka, došlo je do
poremećaja homeostaze fotosintetičkih pigmenta. Najveće promene su uočene u sadržaju
karotenoida, koji predstavljaju važnu komponentnu u neenzimskom odgovoru biljaka na
stres. Koncentracija vodonik-peroksida i stepen lipidne peroksidacije, kod peršuna i
šargarepe, koje su inokulirane imagom, se smanjuju, dok kod celera se ispoljava suprotan
efekat. U početnoj fazi ishrane imaga lisne buve inicijalno povećanje reaktivnih formi kiseonika se neutrališe efikasnom enzimskom antioksidativnom zaštitom, što se ogleda u
povećanoj aktivnosti enzima POX, CAT i SOD, naročito kod peršuna.
PB  - Belgrade: The Plant Protection Society of Serbia
C3  - Zbornik rezimea radova: XVI savetovanje o zaštiti bilja; 2021 Feb 22-25; Zlatibor, Serbia
T1  - Fiziološki i biohemijski odgovori šargarepe, peršuna i celera izazvani ishranom mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera: Psylloidea)
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4905
ER  - 

@conference{
author = "Jerinić-Prodanović, Dušanka and Milošević, Snežana and Đurić, Marija and Jevremović, Slađana and Subotić, Angelina",
year = "2021",
abstract = "Bactericera trigonica je jedna od malobrojnih štetočina koje se javljaju na gajenim
vrstama iz familije Apiaceae. Godišnje može da razvije i do devet generacija, a prisustvo svih
stadijuma razvića na gajenim biljkama je potvrđeno tokom cele vegetacije. Ishranom imaga i
larve ne dovode do vidljivih simptoma oštećenja na napadnutim biljkama. Ukoliko se jave u
povećanoj brojnosti mogu prouzrokovati sušenje pojedinih delova ili čak celih biljaka. Pored
direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Ca. Liberibacter
solanacearum sa zaraženih na zdrave biljke šargarepe i celera. U prirodnim uslovima, kao i pri
uzgoju na poljoprivrednim površinama, biljke su tokom svog životnog ciklusa izložene
promjenjivim uslovima životne sredine koji mogu na različite načine delovati na njihovo
razviće i rastenje. Biotski faktori, poput napada insekata izazivaju promene na svim nivoima
organizacije biljke, od anatomskih i morfoloških osobina do ćelijskog, biohemijskog i
molekularnog nivoa. U eksperiment su bile uključene biljke šargarepe (Daucus carota),
peršuna (Pastinaca sativa) i celera (Apium graveolens), koje smo gajili u saksijama u fitotronu
u kontrolisanim uslovima. U svakoj saksiji se nalazilo po tri biljke. Imaga B. trigonica smo
sakupljali u usevu šargarepe u Futogu, a zatim smo odgajili jednu generaciju na šargarepi u
laboratoriji pri sobnoj temperaturi. Od odgajenih imaga B. trigonica smo formnirali parove po
tri mužjaka i tri ženke, koje smo nanosili na predhodno izolovane biljke sa cilidrima od
markizeta. Ovako izolovane biljke sa nanetim lisnim buvama smo gajili na sobnoj temperaturi.
Radi analize fizioloških i biohemijskih odgovora biljaka, nastalih ishranom imaga i larvi lisne
buve, uzimani su uzorci listova sa svih biljaka. Prvo uzorkovanje je bilo nakon nanošenja
imaga lisne buve, a drugo kada su na biljkama masovno bile larve. Kod biljaka su praćeni
fiziološki parametri (sadržaj fotosintetičkih pigmenata, hlorofila i karotenoida) i biohemijski
parametri (stepen lipidne peroksidacije (MDA), koncetracija vodonik-peroksida (H2O2),
aktivnost oksidativnih enzima, peroksidaza (POX), katalaza (CAT) i super-okisid dismutaza
(SOD)), kao odgovori biljaka na stres indukovan ishranom imaga i larvi lisne buve. Usled
povreda nastalih ishranom lisne buve kod svih eksperimentalnih biljaka, došlo je do
poremećaja homeostaze fotosintetičkih pigmenta. Najveće promene su uočene u sadržaju
karotenoida, koji predstavljaju važnu komponentnu u neenzimskom odgovoru biljaka na
stres. Koncentracija vodonik-peroksida i stepen lipidne peroksidacije, kod peršuna i
šargarepe, koje su inokulirane imagom, se smanjuju, dok kod celera se ispoljava suprotan
efekat. U početnoj fazi ishrane imaga lisne buve inicijalno povećanje reaktivnih formi kiseonika se neutrališe efikasnom enzimskom antioksidativnom zaštitom, što se ogleda u
povećanoj aktivnosti enzima POX, CAT i SOD, naročito kod peršuna.",
publisher = "Belgrade: The Plant Protection Society of Serbia",
journal = "Zbornik rezimea radova: XVI savetovanje o zaštiti bilja; 2021 Feb 22-25; Zlatibor, Serbia",
title = "Fiziološki i biohemijski odgovori šargarepe, peršuna i celera izazvani ishranom mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera: Psylloidea)",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4905"
}

Jerinić-Prodanović, D., Milošević, S., Đurić, M., Jevremović, S.,& Subotić, A.. (2021). Fiziološki i biohemijski odgovori šargarepe, peršuna i celera izazvani ishranom mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera: Psylloidea). in Zbornik rezimea radova: XVI savetovanje o zaštiti bilja; 2021 Feb 22-25; Zlatibor, Serbia
Belgrade: The Plant Protection Society of Serbia., 57.
https://hdl.handle.net/21.15107/rcub_ibiss_4905

Jerinić-Prodanović D, Milošević S, Đurić M, Jevremović S, Subotić A. Fiziološki i biohemijski odgovori šargarepe, peršuna i celera izazvani ishranom mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera: Psylloidea). in Zbornik rezimea radova: XVI savetovanje o zaštiti bilja; 2021 Feb 22-25; Zlatibor, Serbia. 2021;:57.
https://hdl.handle.net/21.15107/rcub_ibiss_4905 .

Jerinić-Prodanović, Dušanka, Milošević, Snežana, Đurić, Marija, Jevremović, Slađana, Subotić, Angelina, "Fiziološki i biohemijski odgovori šargarepe, peršuna i celera izazvani ishranom mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera: Psylloidea)" in Zbornik rezimea radova: XVI savetovanje o zaštiti bilja; 2021 Feb 22-25; Zlatibor, Serbia (2021):57,
https://hdl.handle.net/21.15107/rcub_ibiss_4905 .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Paunović, Danijela
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12593
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3690
AB  - Common centaury (Centaurium erythraea Rafn.) is a plant species that can inhabit saline soils. It is known as a plant with high spontaneous regeneration potential in vitro. In the present work we evaluated shoots and roots salinity tolerance of non-transformed and three AtCKX transgenic centaury lines to graded NaCl concentrations (0, 50, 100, 150, 200 mM) in vitro. Overexpression of AtCKX genes in transgenic centaury plants resulted in an altered cytokinins (CKs) profile leading to a decline of bioactive CK levels and, at the same time, increased contents of storage CK forms, inactive CK forms and/or CK nucleotides. Significant increment of fresh shoot weight was obtained in shoots of non-transformed and AtCKX1 transgenic line only on medium supplemented with 50 mM NaCl. However two analysed AtCKX2 transgenic lines reduced shoot growth at all NaCl concentrations. In general, centaury roots showed higher tolerance to salinity than shoots. Non-transformed and AtCKX1 transgenic lines tolerated up to 100 mM NaCl without change in frequency of regeneration and number of regenerated plants. Roots of two analysed AtCKX2 transgenic lines showed different regeneration potential under salt stress. Regeneration of transgenic AtCKX2-26 shoots even at 200 mM NaCl was recorded. Salinity stress response of centaury shoots and roots was also evaluated at biochemical level. Free proline, malondialdehyde and hydrogen peroxide content as well as antioxidative enzymes activities were investigated in shoots and roots after 1, 2, 4 and 8 weeks. In general, adition of NaCl in culture medium elevated all biochemical parameters in centaury shoots and in roots. Considering that all analysed AtCKX transgenic centaury lines showed altered salt tolerance to graded NaCl concentrations in vitro it can be assumed that CKs might be involved in plant defence to salt stress conditions.
PB  - Blackwell Publishing Ltd
T2  - Annals of Applied Biology
T1  - Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro
DO  - 10.1111/aab.12593
SP  - aab.12593
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Paunović, Danijela and Milošević, Snežana and Marković, Marija and Jevremović, Slađana and Dragićević, Ivana and Subotić, Angelina",
year = "2020",
abstract = "Common centaury (Centaurium erythraea Rafn.) is a plant species that can inhabit saline soils. It is known as a plant with high spontaneous regeneration potential in vitro. In the present work we evaluated shoots and roots salinity tolerance of non-transformed and three AtCKX transgenic centaury lines to graded NaCl concentrations (0, 50, 100, 150, 200 mM) in vitro. Overexpression of AtCKX genes in transgenic centaury plants resulted in an altered cytokinins (CKs) profile leading to a decline of bioactive CK levels and, at the same time, increased contents of storage CK forms, inactive CK forms and/or CK nucleotides. Significant increment of fresh shoot weight was obtained in shoots of non-transformed and AtCKX1 transgenic line only on medium supplemented with 50 mM NaCl. However two analysed AtCKX2 transgenic lines reduced shoot growth at all NaCl concentrations. In general, centaury roots showed higher tolerance to salinity than shoots. Non-transformed and AtCKX1 transgenic lines tolerated up to 100 mM NaCl without change in frequency of regeneration and number of regenerated plants. Roots of two analysed AtCKX2 transgenic lines showed different regeneration potential under salt stress. Regeneration of transgenic AtCKX2-26 shoots even at 200 mM NaCl was recorded. Salinity stress response of centaury shoots and roots was also evaluated at biochemical level. Free proline, malondialdehyde and hydrogen peroxide content as well as antioxidative enzymes activities were investigated in shoots and roots after 1, 2, 4 and 8 weeks. In general, adition of NaCl in culture medium elevated all biochemical parameters in centaury shoots and in roots. Considering that all analysed AtCKX transgenic centaury lines showed altered salt tolerance to graded NaCl concentrations in vitro it can be assumed that CKs might be involved in plant defence to salt stress conditions.",
publisher = "Blackwell Publishing Ltd",
journal = "Annals of Applied Biology",
title = "Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro",
doi = "10.1111/aab.12593",
pages = "aab.12593"
}

Trifunović-Momčilov, M., Paunović, D., Milošević, S., Marković, M., Jevremović, S., Dragićević, I.,& Subotić, A.. (2020). Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro. in Annals of Applied Biology
Blackwell Publishing Ltd., aab.12593.
https://doi.org/10.1111/aab.12593

Trifunović-Momčilov M, Paunović D, Milošević S, Marković M, Jevremović S, Dragićević I, Subotić A. Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro. in Annals of Applied Biology. 2020;:aab.12593.
doi:10.1111/aab.12593 .

Trifunović-Momčilov, Milana, Paunović, Danijela, Milošević, Snežana, Marković, Marija, Jevremović, Slađana, Dragićević, Ivana, Subotić, Angelina, "Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro" in Annals of Applied Biology (2020):aab.12593,
https://doi.org/10.1111/aab.12593 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Radulović, Olga
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1573
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4010
AB  - The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.
PB  - MDPI AG
T2  - Plants
T1  - Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs
IS  - 11
VL  - 9
DO  - 10.3390/plants9111573
SP  - 1573
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Radulović, Olga and Milošević, Snežana and Jevremović, Slađana and Subotić, Angelina",
year = "2020",
abstract = "The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.",
publisher = "MDPI AG",
journal = "Plants",
title = "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs",
number = "11",
volume = "9",
doi = "10.3390/plants9111573",
pages = "1573"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Radulović, O., Milošević, S., Jevremović, S.,& Subotić, A.. (2020). Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. in Plants
MDPI AG., 9(11), 1573.
https://doi.org/10.3390/plants9111573

Marković M, Trifunović-Momčilov M, Uzelac B, Radulović O, Milošević S, Jevremović S, Subotić A. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. in Plants. 2020;9(11):1573.
doi:10.3390/plants9111573 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Radulović, Olga, Milošević, Snežana, Jevremović, Slađana, Subotić, Angelina, "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs" in Plants, 9, no. 11 (2020):1573,
https://doi.org/10.3390/plants9111573 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Cingel, Aleksandar
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1449
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3996
AB  - Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors-ancymidol (A) and paclobutrazol (P)-the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb's dormancy and its sprouting.
PB  - MDPI AG
T2  - Plants (Basel, Switzerland)
T1  - Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.
IS  - 11
VL  - 9
DO  - 10.3390/plants9111449
SP  - 1449
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Cingel, Aleksandar and Milošević, Snežana and Jevremović, Slađana and Subotić, Angelina",
year = "2020",
abstract = "Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well as in vitro, they become dormant and require low temperatures for further growth during the next vegetative period. In the present study, using 10 μM of gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors-ancymidol (A) and paclobutrazol (P)-the dynamic changes in soluble sugars, fructose and glucose content, fresh weight and sprouting capacity were investigated. F. meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1, 2 and 5 weeks at two different temperatures (24 and 7 °C). GA3 improved bulb fresh weight, as well as sprouting percentage at both tested temperatures, compared to the control. The highest fresh weight increase (57.7%) and sprouting rate (29.02%) were achieved when bulbs were grown at 24 °C for 5 weeks. In addition, soluble sugar content was the highest in bulbs grown for 5 weeks on medium supplemented with GA3. The main sugar in fritillary bulbs was glucose, while fructose content was lower. The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs. To our knowledge, this is the first study of the sugar composition in F. meleagris bulbs during breaking of the bulb's dormancy and its sprouting.",
publisher = "MDPI AG",
journal = "Plants (Basel, Switzerland)",
title = "Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.",
number = "11",
volume = "9",
doi = "10.3390/plants9111449",
pages = "1449"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Cingel, A., Milošević, S., Jevremović, S.,& Subotić, A.. (2020). Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.. in Plants (Basel, Switzerland)
MDPI AG., 9(11), 1449.
https://doi.org/10.3390/plants9111449

Marković M, Trifunović-Momčilov M, Uzelac B, Cingel A, Milošević S, Jevremović S, Subotić A. Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content.. in Plants (Basel, Switzerland). 2020;9(11):1449.
doi:10.3390/plants9111449 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Cingel, Aleksandar, Milošević, Snežana, Jevremović, Slađana, Subotić, Angelina, "Breaking the Dormancy of Snake's Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs-Part 1: Effect of GA3, GA Inhibitors and Temperature on Fresh Weight, Sprouting and Sugar Content." in Plants (Basel, Switzerland), 9, no. 11 (2020):1449,
https://doi.org/10.3390/plants9111449 . .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Milutinović, Milica
AU  - Puač, Nevena
AU  - Petrović, Zoran Lj
AU  - Škoro, Nikola
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4398
AB  - The expansion of plasma medicine and its demand for precise and localized in vivo treatments of living cells and tissues resulted in the fast development of various plasma devices that operate at atmospheric pressure. In the present study, plant undifferentiated compact tissue (calli) of Balkan endemic small bearded iris (Iris reichenbachii Heuff.) was treated using a plasma needle device designed for biomedical applications and tested with numerous diagnostic procedures [1]. Significant morphological alterations of the iris calli were observed after direct plasma treatment. The indicated changes evaluated at the surface of the calli tissue after prolonged culture could be attributed to an enhanced cell division (mitotic activity) of the plant cells and differentiation of friable calli stimulated by low-temperature plasma. The current morphological changes were followed by the significant shift in calli physiology. The results showed that the plasma treatment enhanced the accumulation of specific arabinogalactan proteins in the epidermal cells and extracellular space of the iris calli. At the same time, the plant antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), that represent the main lines of plant cell defence against oxidative stress, exhibited different pattern of expression depending on the plasma treatment. The present study outlines that the plasma technique could be applied as an alternative and valuable approach for promoting regeneration and multiplication in a plant meristematic tissue culture.
PB  - Heron Press
C3  - Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)
T1  - Alteration in plant tissue morphology and physiology induced by plasma treatment
SP  - 63
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4398
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Milutinović, Milica and Puač, Nevena and Petrović, Zoran Lj and Škoro, Nikola",
year = "2019",
abstract = "The expansion of plasma medicine and its demand for precise and localized in vivo treatments of living cells and tissues resulted in the fast development of various plasma devices that operate at atmospheric pressure. In the present study, plant undifferentiated compact tissue (calli) of Balkan endemic small bearded iris (Iris reichenbachii Heuff.) was treated using a plasma needle device designed for biomedical applications and tested with numerous diagnostic procedures [1]. Significant morphological alterations of the iris calli were observed after direct plasma treatment. The indicated changes evaluated at the surface of the calli tissue after prolonged culture could be attributed to an enhanced cell division (mitotic activity) of the plant cells and differentiation of friable calli stimulated by low-temperature plasma. The current morphological changes were followed by the significant shift in calli physiology. The results showed that the plasma treatment enhanced the accumulation of specific arabinogalactan proteins in the epidermal cells and extracellular space of the iris calli. At the same time, the plant antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), that represent the main lines of plant cell defence against oxidative stress, exhibited different pattern of expression depending on the plasma treatment. The present study outlines that the plasma technique could be applied as an alternative and valuable approach for promoting regeneration and multiplication in a plant meristematic tissue culture.",
publisher = "Heron Press",
journal = "Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)",
title = "Alteration in plant tissue morphology and physiology induced by plasma treatment",
pages = "63-64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4398"
}

Živković, S., Jevremović, S., Milutinović, M., Puač, N., Petrović, Z. L.,& Škoro, N.. (2019). Alteration in plant tissue morphology and physiology induced by plasma treatment. in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)
Heron Press., 63-64.
https://hdl.handle.net/21.15107/rcub_ibiss_4398

Živković S, Jevremović S, Milutinović M, Puač N, Petrović ZL, Škoro N. Alteration in plant tissue morphology and physiology induced by plasma treatment. in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019). 2019;:63-64.
https://hdl.handle.net/21.15107/rcub_ibiss_4398 .

Živković, Suzana, Jevremović, Slađana, Milutinović, Milica, Puač, Nevena, Petrović, Zoran Lj, Škoro, Nikola, "Alteration in plant tissue morphology and physiology induced by plasma treatment" in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019) (2019):63-64,
https://hdl.handle.net/21.15107/rcub_ibiss_4398 .

TY  - JOUR
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Cingel, Aleksandar
AU  - Ghalawenji, Nabil
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2019
UR  - http://link.springer.com/10.1007/s13205-018-1540-4
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6314937
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3232
AB  - The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.
T2  - 3 Biotech
T1  - Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.
IS  - 1
VL  - 9
DO  - 10.1007/s13205-018-1540-4
SP  - 17
ER  - 

@article{
author = "Trajković, Milena and Antonić Reljin, Dragana and Cingel, Aleksandar and Ghalawenji, Nabil and Subotić, Angelina and Jevremović, Slađana",
year = "2019",
abstract = "The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.",
journal = "3 Biotech",
title = "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.",
number = "1",
volume = "9",
doi = "10.1007/s13205-018-1540-4",
pages = "17"
}

Trajković, M., Antonić Reljin, D., Cingel, A., Ghalawenji, N., Subotić, A.,& Jevremović, S.. (2019). Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech, 9(1), 17.
https://doi.org/10.1007/s13205-018-1540-4

Trajković M, Antonić Reljin D, Cingel A, Ghalawenji N, Subotić A, Jevremović S. Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech. 2019;9(1):17.
doi:10.1007/s13205-018-1540-4 .

Trajković, Milena, Antonić Reljin, Dragana, Cingel, Aleksandar, Ghalawenji, Nabil, Subotić, Angelina, Jevremović, Slađana, "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta." in 3 Biotech, 9, no. 1 (2019):17,
https://doi.org/10.1007/s13205-018-1540-4 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Krstić Milošević, Dijana
AU  - Trifunović, Snežana
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2019
UR  - http://eemj.eu/index.php/EEMJ/article/view/3962
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3592
AB  - Common centaury, Centaurium erythraea Rafn., represent the best known and the most investigated medicinal plant species of genus Centaurium. Centaury has been used for centuries in traditional medicine. Secondary metabolites such as bitter secoiridoid glucosides (gentiopicrin, swertiamarin and sweroside), xanthones (eustomin and demethyleustomin), and phenolic acids are the main constituents responsible for the therapeutic properties of centaury. Previous investigation showed that overexpression of the AtCKX genes in transgenic centaury plants did not result in a decrease of total cytokinin (CK) content, but in an altered CK profile leading to a decline of bioactive, the most important physiologically active group of CKs.The aim of this study was to investigate antibacterial and antifungal activity of transgenic centaury methanol extracts as well as pure secoiridoid and xanthone compounds on four Gram positive, four Gram negative bacteria and eight species of microfungi. All tested methanol extracts of control and transgenic AtCKX1 centaury shoots and roots showed better antibacterial activity, while pure compounds (gentiopicrin, swertiamarin, eustomin and demethyleustomin) showed better antifungal activity. The results obtained in this work suggest that centaury methanol extracts and pure compounds represent potential antimicrobials confirming the possibility of using these compounds in agronomy, veterinary, medicine or food industry.
PB  - Gheorghe Asachi Technical University of Iasi, Romania
T2  - Environmental Engineering and Management Journal
T1  - Antimicrobial activity, antioxidant potential and total phenolic content of transgenic ATCKX1 centaury (Centaurium erythraea Rafn.) Plants grown in vitro
IS  - 9
VL  - 18
SP  - 2063
EP  - 2072
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3592
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Krstić Milošević, Dijana and Trifunović, Snežana and Ćirić, Ana and Glamočlija, Jasmina and Jevremović, Slađana and Subotić, Angelina",
year = "2019",
abstract = "Common centaury, Centaurium erythraea Rafn., represent the best known and the most investigated medicinal plant species of genus Centaurium. Centaury has been used for centuries in traditional medicine. Secondary metabolites such as bitter secoiridoid glucosides (gentiopicrin, swertiamarin and sweroside), xanthones (eustomin and demethyleustomin), and phenolic acids are the main constituents responsible for the therapeutic properties of centaury. Previous investigation showed that overexpression of the AtCKX genes in transgenic centaury plants did not result in a decrease of total cytokinin (CK) content, but in an altered CK profile leading to a decline of bioactive, the most important physiologically active group of CKs.The aim of this study was to investigate antibacterial and antifungal activity of transgenic centaury methanol extracts as well as pure secoiridoid and xanthone compounds on four Gram positive, four Gram negative bacteria and eight species of microfungi. All tested methanol extracts of control and transgenic AtCKX1 centaury shoots and roots showed better antibacterial activity, while pure compounds (gentiopicrin, swertiamarin, eustomin and demethyleustomin) showed better antifungal activity. The results obtained in this work suggest that centaury methanol extracts and pure compounds represent potential antimicrobials confirming the possibility of using these compounds in agronomy, veterinary, medicine or food industry.",
publisher = "Gheorghe Asachi Technical University of Iasi, Romania",
journal = "Environmental Engineering and Management Journal",
title = "Antimicrobial activity, antioxidant potential and total phenolic content of transgenic ATCKX1 centaury (Centaurium erythraea Rafn.) Plants grown in vitro",
number = "9",
volume = "18",
pages = "2063-2072",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3592"
}

Trifunović-Momčilov, M., Krstić Milošević, D., Trifunović, S., Ćirić, A., Glamočlija, J., Jevremović, S.,& Subotić, A.. (2019). Antimicrobial activity, antioxidant potential and total phenolic content of transgenic ATCKX1 centaury (Centaurium erythraea Rafn.) Plants grown in vitro. in Environmental Engineering and Management Journal
Gheorghe Asachi Technical University of Iasi, Romania., 18(9), 2063-2072.
https://hdl.handle.net/21.15107/rcub_ibiss_3592

Trifunović-Momčilov M, Krstić Milošević D, Trifunović S, Ćirić A, Glamočlija J, Jevremović S, Subotić A. Antimicrobial activity, antioxidant potential and total phenolic content of transgenic ATCKX1 centaury (Centaurium erythraea Rafn.) Plants grown in vitro. in Environmental Engineering and Management Journal. 2019;18(9):2063-2072.
https://hdl.handle.net/21.15107/rcub_ibiss_3592 .

Trifunović-Momčilov, Milana, Krstić Milošević, Dijana, Trifunović, Snežana, Ćirić, Ana, Glamočlija, Jasmina, Jevremović, Slađana, Subotić, Angelina, "Antimicrobial activity, antioxidant potential and total phenolic content of transgenic ATCKX1 centaury (Centaurium erythraea Rafn.) Plants grown in vitro" in Environmental Engineering and Management Journal, 18, no. 9 (2019):2063-2072,
https://hdl.handle.net/21.15107/rcub_ibiss_3592 .