TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Katanić Stanković, Jelena S.
AU  - Jurić, Tatjana
AU  - Srećković, Nikola
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Monti, Daria Maria
AU  - Imbimbo, Paola
AU  - Nikles, Stefanie
AU  - Pan, San-Po
AU  - Bauer, Rudolf
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0926669019309847?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3562
AB  - Blackstonia perfoliata (L.) Huds. is known as a highly bitter secoiridoid glycosides-containing plant and as a possible substitute for some Gentianaceae plants in herbal preparations. Nevertheless, its bioactive properties are still unknown. The present study aimed to characterize both, secoiridoid glycosides and phenolic constituents, and to investigate antioxidant, antimicrobial, anti-inflammatory, and cytotoxic activities of B. perfoliata methanolic extract. The secoiridoid glycosides swertiamarin, gentiopicrin, and sweroside were found to be the dominant compounds of the extract, while 23 phenolic compounds were identified in much lower concentrations. Among phenolics, flavanols were the most abundant, which represents a unique feature among Gentianaceae species. The extract showed moderate to weak antioxidant activity with better performance in inhibition of lipid peroxidation than in free radical scavenging activities. The extract showed generally better antifungal properties compared with its antibacterial potential. Also, B. perfoliata demonstrated in vitro anti-inflammatory activity, the extract (50 μg/mL) showed inhibition of cyclooxigenases, COX-1 and COX-2, activities (19.65 and 48.02%, respectively). It also displayed biocompatibility on the immortalized and cancer cells, as no cytotoxic effect was observed. For the first time, the bioactive potential of this species was demonstrated, justifying its usage in pharmaceutical and food products as an alternative for some overexploited and endangered species from the Gentianaceae family.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Blackstonia perfoliata (L.) Huds. (Gentianaceae): A promising source of useful bioactive compounds
VL  - 145
DO  - 10.1016/J.INDCROP.2019.111974
SP  - 111974
ER  - 

@article{
author = "Mihailović, Vladimir and Katanić Stanković, Jelena S. and Jurić, Tatjana and Srećković, Nikola and Mišić, Danijela and Šiler, Branislav and Monti, Daria Maria and Imbimbo, Paola and Nikles, Stefanie and Pan, San-Po and Bauer, Rudolf",
year = "2020",
abstract = "Blackstonia perfoliata (L.) Huds. is known as a highly bitter secoiridoid glycosides-containing plant and as a possible substitute for some Gentianaceae plants in herbal preparations. Nevertheless, its bioactive properties are still unknown. The present study aimed to characterize both, secoiridoid glycosides and phenolic constituents, and to investigate antioxidant, antimicrobial, anti-inflammatory, and cytotoxic activities of B. perfoliata methanolic extract. The secoiridoid glycosides swertiamarin, gentiopicrin, and sweroside were found to be the dominant compounds of the extract, while 23 phenolic compounds were identified in much lower concentrations. Among phenolics, flavanols were the most abundant, which represents a unique feature among Gentianaceae species. The extract showed moderate to weak antioxidant activity with better performance in inhibition of lipid peroxidation than in free radical scavenging activities. The extract showed generally better antifungal properties compared with its antibacterial potential. Also, B. perfoliata demonstrated in vitro anti-inflammatory activity, the extract (50 μg/mL) showed inhibition of cyclooxigenases, COX-1 and COX-2, activities (19.65 and 48.02%, respectively). It also displayed biocompatibility on the immortalized and cancer cells, as no cytotoxic effect was observed. For the first time, the bioactive potential of this species was demonstrated, justifying its usage in pharmaceutical and food products as an alternative for some overexploited and endangered species from the Gentianaceae family.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Blackstonia perfoliata (L.) Huds. (Gentianaceae): A promising source of useful bioactive compounds",
volume = "145",
doi = "10.1016/J.INDCROP.2019.111974",
pages = "111974"
}

Mihailović, V., Katanić Stanković, J. S., Jurić, T., Srećković, N., Mišić, D., Šiler, B., Monti, D. M., Imbimbo, P., Nikles, S., Pan, S.,& Bauer, R.. (2020). Blackstonia perfoliata (L.) Huds. (Gentianaceae): A promising source of useful bioactive compounds. in Industrial Crops and Products
Elsevier., 145, 111974.
https://doi.org/10.1016/J.INDCROP.2019.111974

Mihailović V, Katanić Stanković JS, Jurić T, Srećković N, Mišić D, Šiler B, Monti DM, Imbimbo P, Nikles S, Pan S, Bauer R. Blackstonia perfoliata (L.) Huds. (Gentianaceae): A promising source of useful bioactive compounds. in Industrial Crops and Products. 2020;145:111974.
doi:10.1016/J.INDCROP.2019.111974 .

Mihailović, Vladimir, Katanić Stanković, Jelena S., Jurić, Tatjana, Srećković, Nikola, Mišić, Danijela, Šiler, Branislav, Monti, Daria Maria, Imbimbo, Paola, Nikles, Stefanie, Pan, San-Po, Bauer, Rudolf, "Blackstonia perfoliata (L.) Huds. (Gentianaceae): A promising source of useful bioactive compounds" in Industrial Crops and Products, 145 (2020):111974,
https://doi.org/10.1016/J.INDCROP.2019.111974 . .

TY  - JOUR
AU  - Jurić, Tatjana
AU  - Katanić Stanković, Jelena S.
AU  - Rosić, Gvozden
AU  - Selaković, Dragica
AU  - Joksimović, Jovana
AU  - Mišić, Danijela
AU  - Stanković, Vesna
AU  - Mihailović, Vladimir
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0254629919309962?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3522
AB  - This study was designed to evaluate the possible effect of methanol extracts of aerial parts and roots of Alchemilla vulgaris L. (AVA and AVR, respectively) in preventing cisplatin-induced hepatorenal and testicular toxicity in rats. UHPLC/DAD/(−)HESI-MS/MS analysis was performed to determine the detailed phenolic profile of AVA and AVR. The male Wistar rats were orally treated with extracts at three different concentrations (50, 100, and 200 mg/kg b.w.) for 10 days and toxicity was induced by injection of single dose of cisplatin on the 5th day (7.5 mg/kg b.w.). Determination of serum biochemical markers of hepatorenal and testicular tissue injury, as well as oxidative-stress parameters in tissues and histopathological study, were performed. Treatments with AVA and AVR significantly attenuated the levels of serum parameters of liver, kidneys and testicles injury, tissue's morphology and parameters of oxidative stress caused by an application of the cisplatin. Chromatographic analysis showed the presence of more than 20 different phenolic compounds in extracts where ellagic acid, catechin, and catechin gallate were dominant components in both extracts. The obtained results indicate that A. vulgaris extracts may be used in preventing cisplatin-induced toxicity during chemotherapy as well as in the treatment of oxidative stress-related disorders.
T2  - South African Journal of Botany
T1  - Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats
VL  - 128
DO  - 10.1016/J.SAJB.2019.09.010
SP  - 141
EP  - 151
ER  - 

@article{
author = "Jurić, Tatjana and Katanić Stanković, Jelena S. and Rosić, Gvozden and Selaković, Dragica and Joksimović, Jovana and Mišić, Danijela and Stanković, Vesna and Mihailović, Vladimir",
year = "2020",
abstract = "This study was designed to evaluate the possible effect of methanol extracts of aerial parts and roots of Alchemilla vulgaris L. (AVA and AVR, respectively) in preventing cisplatin-induced hepatorenal and testicular toxicity in rats. UHPLC/DAD/(−)HESI-MS/MS analysis was performed to determine the detailed phenolic profile of AVA and AVR. The male Wistar rats were orally treated with extracts at three different concentrations (50, 100, and 200 mg/kg b.w.) for 10 days and toxicity was induced by injection of single dose of cisplatin on the 5th day (7.5 mg/kg b.w.). Determination of serum biochemical markers of hepatorenal and testicular tissue injury, as well as oxidative-stress parameters in tissues and histopathological study, were performed. Treatments with AVA and AVR significantly attenuated the levels of serum parameters of liver, kidneys and testicles injury, tissue's morphology and parameters of oxidative stress caused by an application of the cisplatin. Chromatographic analysis showed the presence of more than 20 different phenolic compounds in extracts where ellagic acid, catechin, and catechin gallate were dominant components in both extracts. The obtained results indicate that A. vulgaris extracts may be used in preventing cisplatin-induced toxicity during chemotherapy as well as in the treatment of oxidative stress-related disorders.",
journal = "South African Journal of Botany",
title = "Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats",
volume = "128",
doi = "10.1016/J.SAJB.2019.09.010",
pages = "141-151"
}

Jurić, T., Katanić Stanković, J. S., Rosić, G., Selaković, D., Joksimović, J., Mišić, D., Stanković, V.,& Mihailović, V.. (2020). Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats. in South African Journal of Botany, 128, 141-151.
https://doi.org/10.1016/J.SAJB.2019.09.010

Jurić T, Katanić Stanković JS, Rosić G, Selaković D, Joksimović J, Mišić D, Stanković V, Mihailović V. Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats. in South African Journal of Botany. 2020;128:141-151.
doi:10.1016/J.SAJB.2019.09.010 .

Jurić, Tatjana, Katanić Stanković, Jelena S., Rosić, Gvozden, Selaković, Dragica, Joksimović, Jovana, Mišić, Danijela, Stanković, Vesna, Mihailović, Vladimir, "Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats" in South African Journal of Botany, 128 (2020):141-151,
https://doi.org/10.1016/J.SAJB.2019.09.010 . .

TY  - JOUR
AU  - Katanić Stanković, Jelena S.
AU  - Srećković, Nikola
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Imbimbo, Paola
AU  - Monti, Daria Maria
AU  - Mihailović, Vladimir
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0926669019309422?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3523
AB  - The plants from genus Salvia, as one of the largest genus in Lamiaceae family, are frequently in use for various purposes, as foods, in cosmetic industry, or in traditional and official medicine. Salvia verticillata L. (liliac sage) is one of sidelined sage species with potential bioactivity, reported in traditional medicine. The aim of this study was to acquire a phytochemical profile of the methanol extract obtained from S. verticillata aerial parts and to evaluate its antioxidant, antimicrobial, and biocompatibility potential. Characteristic compounds of the genus Salvia, such as rosmarinic and caffeic acids, along with their derivatives (e.g. salvianolic and yunnaneic acids isomers) and flavonoids, have been identified by ultrahigh-performance Orbitrap metabolomic fingerprinting as the main phenolic metabolites in S. verticillata. The extract displayed moderate antimicrobial properties and significant antioxidant potential, with the half maximal inhibitory concentration values (IC50) ranging from 33 to 73 μg/mL. Importantly, full biocompatibility of the extract with eukaryotic cell lines was observed up to 72 h. The obtained results revealed the presence of polyphenolic bioactive compounds in S. verticillata extract with promising antioxidant potential and significant biocompatibility. In this regard, S. verticillata can find new perspectives of application as a food ingredient, in cosmetic and pharmaceutical industries, as it represents a valuable source of compounds with prominent health properties, with a special focus on rosmarinic acid.
T2  - Industrial Crops and Products
T1  - Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid
VL  - 143
DO  - 10.1016/J.INDCROP.2019.111932
SP  - 111932
ER  - 

@article{
author = "Katanić Stanković, Jelena S. and Srećković, Nikola and Mišić, Danijela and Gašić, Uroš and Imbimbo, Paola and Monti, Daria Maria and Mihailović, Vladimir",
year = "2020",
abstract = "The plants from genus Salvia, as one of the largest genus in Lamiaceae family, are frequently in use for various purposes, as foods, in cosmetic industry, or in traditional and official medicine. Salvia verticillata L. (liliac sage) is one of sidelined sage species with potential bioactivity, reported in traditional medicine. The aim of this study was to acquire a phytochemical profile of the methanol extract obtained from S. verticillata aerial parts and to evaluate its antioxidant, antimicrobial, and biocompatibility potential. Characteristic compounds of the genus Salvia, such as rosmarinic and caffeic acids, along with their derivatives (e.g. salvianolic and yunnaneic acids isomers) and flavonoids, have been identified by ultrahigh-performance Orbitrap metabolomic fingerprinting as the main phenolic metabolites in S. verticillata. The extract displayed moderate antimicrobial properties and significant antioxidant potential, with the half maximal inhibitory concentration values (IC50) ranging from 33 to 73 μg/mL. Importantly, full biocompatibility of the extract with eukaryotic cell lines was observed up to 72 h. The obtained results revealed the presence of polyphenolic bioactive compounds in S. verticillata extract with promising antioxidant potential and significant biocompatibility. In this regard, S. verticillata can find new perspectives of application as a food ingredient, in cosmetic and pharmaceutical industries, as it represents a valuable source of compounds with prominent health properties, with a special focus on rosmarinic acid.",
journal = "Industrial Crops and Products",
title = "Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid",
volume = "143",
doi = "10.1016/J.INDCROP.2019.111932",
pages = "111932"
}

Katanić Stanković, J. S., Srećković, N., Mišić, D., Gašić, U., Imbimbo, P., Monti, D. M.,& Mihailović, V.. (2020). Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid. in Industrial Crops and Products, 143, 111932.
https://doi.org/10.1016/J.INDCROP.2019.111932

Katanić Stanković JS, Srećković N, Mišić D, Gašić U, Imbimbo P, Monti DM, Mihailović V. Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid. in Industrial Crops and Products. 2020;143:111932.
doi:10.1016/J.INDCROP.2019.111932 .

Katanić Stanković, Jelena S., Srećković, Nikola, Mišić, Danijela, Gašić, Uroš, Imbimbo, Paola, Monti, Daria Maria, Mihailović, Vladimir, "Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid" in Industrial Crops and Products, 143 (2020):111932,
https://doi.org/10.1016/J.INDCROP.2019.111932 . .

TY  - JOUR
AU  - Boroja, Tatjana
AU  - Katanić, Jelena
AU  - Rosić, Gvozden
AU  - Selaković, Dragica
AU  - Joksimović, Jovana
AU  - Mišić, Danijela
AU  - Stanković, Vesna
AU  - Jovičić, Nemanja
AU  - Mihailović, Vladimir
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0278691518302904?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3066
AB  - The aim of our study was to examine the potential ameliorating effect of the methanolic extract of Satureja hortensis L. (summer savory) aerial parts against cisplatin-induced oxidative damage in renal, hepatic, and testicular tissues. S. hortensis methanol extract at the doses of 50, 100 and 200 mg/kg of body weight were orally administered to Wistar rats once daily for 10 days. Toxicity was induced by intraperitoneal injection of a single dose of cisplatin (7.5 mg/kg of body weight) on the 5th day of the experiment. Applied treatment with S. hortensis extract restored tissue morphology, ameliorated levels of serum parameters for liver, renal and testes function, tissue oxidative stress parameters, and increased Bcl-2/Bax ratio as an indicator of apoptosis in experimental animals caused by application of cisplatin. UHPLC/DAD/HESI-MS/MS analysis revealed that S. hortensis extract was rich in phenolic compounds with rosmarinic acid (24.9 mg/g) as the main compound, followed by caffeic acid (1.28 mg/g) and naringenin (1.06 mg/g). Our findings suggest that S. hortensis may be a valuable source of dietary and pharmacologically important phenolic compounds, especially rosmarinic acid, in pharmaceutical and functional food formulations in order to maintain normal health conditions or as a remedy in various diseases caused by oxidative damage.
T2  - Food and Chemical Toxicology
T1  - Summer savory (Satureja hortensis L.) extract: Phytochemical profile and modulation of cisplatin-induced liver, renal and testicular toxicity.
VL  - 118
DO  - 10.1016/j.fct.2018.05.001
SP  - 252
EP  - 263
ER  - 

@article{
author = "Boroja, Tatjana and Katanić, Jelena and Rosić, Gvozden and Selaković, Dragica and Joksimović, Jovana and Mišić, Danijela and Stanković, Vesna and Jovičić, Nemanja and Mihailović, Vladimir",
year = "2018",
abstract = "The aim of our study was to examine the potential ameliorating effect of the methanolic extract of Satureja hortensis L. (summer savory) aerial parts against cisplatin-induced oxidative damage in renal, hepatic, and testicular tissues. S. hortensis methanol extract at the doses of 50, 100 and 200 mg/kg of body weight were orally administered to Wistar rats once daily for 10 days. Toxicity was induced by intraperitoneal injection of a single dose of cisplatin (7.5 mg/kg of body weight) on the 5th day of the experiment. Applied treatment with S. hortensis extract restored tissue morphology, ameliorated levels of serum parameters for liver, renal and testes function, tissue oxidative stress parameters, and increased Bcl-2/Bax ratio as an indicator of apoptosis in experimental animals caused by application of cisplatin. UHPLC/DAD/HESI-MS/MS analysis revealed that S. hortensis extract was rich in phenolic compounds with rosmarinic acid (24.9 mg/g) as the main compound, followed by caffeic acid (1.28 mg/g) and naringenin (1.06 mg/g). Our findings suggest that S. hortensis may be a valuable source of dietary and pharmacologically important phenolic compounds, especially rosmarinic acid, in pharmaceutical and functional food formulations in order to maintain normal health conditions or as a remedy in various diseases caused by oxidative damage.",
journal = "Food and Chemical Toxicology",
title = "Summer savory (Satureja hortensis L.) extract: Phytochemical profile and modulation of cisplatin-induced liver, renal and testicular toxicity.",
volume = "118",
doi = "10.1016/j.fct.2018.05.001",
pages = "252-263"
}

Boroja, T., Katanić, J., Rosić, G., Selaković, D., Joksimović, J., Mišić, D., Stanković, V., Jovičić, N.,& Mihailović, V.. (2018). Summer savory (Satureja hortensis L.) extract: Phytochemical profile and modulation of cisplatin-induced liver, renal and testicular toxicity.. in Food and Chemical Toxicology, 118, 252-263.
https://doi.org/10.1016/j.fct.2018.05.001

Boroja T, Katanić J, Rosić G, Selaković D, Joksimović J, Mišić D, Stanković V, Jovičić N, Mihailović V. Summer savory (Satureja hortensis L.) extract: Phytochemical profile and modulation of cisplatin-induced liver, renal and testicular toxicity.. in Food and Chemical Toxicology. 2018;118:252-263.
doi:10.1016/j.fct.2018.05.001 .

Boroja, Tatjana, Katanić, Jelena, Rosić, Gvozden, Selaković, Dragica, Joksimović, Jovana, Mišić, Danijela, Stanković, Vesna, Jovičić, Nemanja, Mihailović, Vladimir, "Summer savory (Satureja hortensis L.) extract: Phytochemical profile and modulation of cisplatin-induced liver, renal and testicular toxicity." in Food and Chemical Toxicology, 118 (2018):252-263,
https://doi.org/10.1016/j.fct.2018.05.001 . .

TY  - JOUR
AU  - Katanić, Jelena
AU  - Matić, Sanja
AU  - Pferschy-Wenzig, Eva-Maria
AU  - Kretschmer, Nadine
AU  - Boroja, Tatjana
AU  - Mihailović, Vladimir
AU  - Stanković, Vesna
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Bauer, Rudolf
PY  - 2017
UR  - https://www.sciencedirect.com/science/article/pii/S0278691516304343?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3178
AB  - Filipendula ulmaria, known as meadowsweet, is a perennial herb found in wild and cultivated habitats in Europe and Asia. Usage of F. ulmaria in traditional medicine is based on diuretic, astringent, antirheumatic, and anti-inflammatory properties of this plant. Exposure to cisplatin at a dose of 7.5 mg/kg caused significant increase in serum parameters of liver and kidneys function and tissue oxidative stress markers along with some histopathological changes in liver and kidney tissues of experimental rats, as well as high level of genotoxicity. Administration of F. ulmaria extracts in three different concentrations (100, 200, and 400 mg/kg/day) for 10 days resulted in a reduction of oxidative stress in tissues and decrease of serum parameters. Moreover, tested extracts attenuated the genotoxicity of cisplatin in reverse dose-dependent manner. F. ulmaria extracts had no in vitro cytotoxic activity at all applied concentrations (IC50 > 50 μg/mL). Tested extracts, rich in polyphenolic compounds, attenuate cisplatin-induced liver and kidney oxidative stress, reduce tissue damage, and enhance the antioxidative status of experimental animals during cisplatin application. Therefore, F. ulmaria extracts may be used as supportive agent for the prevention and amelioration of cisplatin side effects.
T2  - Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association
T1  - Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.
VL  - 99
DO  - 10.1016/j.fct.2016.11.018
SP  - 86
EP  - 102
ER  - 

@article{
author = "Katanić, Jelena and Matić, Sanja and Pferschy-Wenzig, Eva-Maria and Kretschmer, Nadine and Boroja, Tatjana and Mihailović, Vladimir and Stanković, Vesna and Stanković, Nevena and Mladenović, Milan and Stanić, Snežana and Mihailović, Mirjana and Bauer, Rudolf",
year = "2017",
abstract = "Filipendula ulmaria, known as meadowsweet, is a perennial herb found in wild and cultivated habitats in Europe and Asia. Usage of F. ulmaria in traditional medicine is based on diuretic, astringent, antirheumatic, and anti-inflammatory properties of this plant. Exposure to cisplatin at a dose of 7.5 mg/kg caused significant increase in serum parameters of liver and kidneys function and tissue oxidative stress markers along with some histopathological changes in liver and kidney tissues of experimental rats, as well as high level of genotoxicity. Administration of F. ulmaria extracts in three different concentrations (100, 200, and 400 mg/kg/day) for 10 days resulted in a reduction of oxidative stress in tissues and decrease of serum parameters. Moreover, tested extracts attenuated the genotoxicity of cisplatin in reverse dose-dependent manner. F. ulmaria extracts had no in vitro cytotoxic activity at all applied concentrations (IC50 > 50 μg/mL). Tested extracts, rich in polyphenolic compounds, attenuate cisplatin-induced liver and kidney oxidative stress, reduce tissue damage, and enhance the antioxidative status of experimental animals during cisplatin application. Therefore, F. ulmaria extracts may be used as supportive agent for the prevention and amelioration of cisplatin side effects.",
journal = "Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association",
title = "Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.",
volume = "99",
doi = "10.1016/j.fct.2016.11.018",
pages = "86-102"
}

Katanić, J., Matić, S., Pferschy-Wenzig, E., Kretschmer, N., Boroja, T., Mihailović, V., Stanković, V., Stanković, N., Mladenović, M., Stanić, S., Mihailović, M.,& Bauer, R.. (2017). Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.. in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association, 99, 86-102.
https://doi.org/10.1016/j.fct.2016.11.018

Katanić J, Matić S, Pferschy-Wenzig E, Kretschmer N, Boroja T, Mihailović V, Stanković V, Stanković N, Mladenović M, Stanić S, Mihailović M, Bauer R. Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.. in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association. 2017;99:86-102.
doi:10.1016/j.fct.2016.11.018 .

Katanić, Jelena, Matić, Sanja, Pferschy-Wenzig, Eva-Maria, Kretschmer, Nadine, Boroja, Tatjana, Mihailović, Vladimir, Stanković, Vesna, Stanković, Nevena, Mladenović, Milan, Stanić, Snežana, Mihailović, Mirjana, Bauer, Rudolf, "Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis." in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association, 99 (2017):86-102,
https://doi.org/10.1016/j.fct.2016.11.018 . .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstić, Miodrag
AU  - Spasić, Snežana
AU  - Miletić, Srdjan
AU  - Stefanović-Kojić, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0963996917304787
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2843
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Fourier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin-derived oligosaccharides on hydroxyl radical (HO)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus aureus in the presence of dietary-relevant HO-generating system (iron+ascorbate). The oligosaccharides react with HO radical to produce carbon dioxide radical anion (CO 2 - ). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO 2 - , which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO.
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstić, Miodrag and Spasić, Snežana and Miletić, Srdjan and Stefanović-Kojić, Jovana and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Fourier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin-derived oligosaccharides on hydroxyl radical (HO)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus aureus in the presence of dietary-relevant HO-generating system (iron+ascorbate). The oligosaccharides react with HO radical to produce carbon dioxide radical anion (CO 2 - ). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO 2 - , which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO.",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstić, M., Spasić, S., Miletić, S., Stefanović-Kojić, J., Nikolić-Kokić, A., Blagojević, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstić M, Spasić S, Miletić S, Stefanović-Kojić J, Nikolić-Kokić A, Blagojević D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstić, Miodrag, Spasić, Snežana, Miletić, Srdjan, Stefanović-Kojić, Jovana, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International (2017),
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - JOUR
AU  - Milošev, Milorad Z
AU  - Jakovljević, Katarina
AU  - Joksović, Milan D
AU  - Stanojković, Tatjana
AU  - Matić, Ivana Z
AU  - Perović, Milka
AU  - Tešić, Vesna
AU  - Kanazir, Selma
AU  - Mladenović, Milan
AU  - Rodić, Marko V
AU  - Leovac, Vukadin M
AU  - Trifunović, Snežana
AU  - Marković, Violeta
PY  - 2017
UR  - http://doi.wiley.com/10.1111/cbdd.12920
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27933733
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2753
AB  - A series of 18 novel N-Mannich bases derived from 5-adamantyl-1,2,4-triazole-3-thione was synthesized and characterized using NMR spectroscopy and X-ray diffraction technique. All derivatives were evaluated for their anticancer potential against four human cancer cell lines. Several tested compounds exerted good cytotoxic activities on K562 and HL-60 cell lines, along with pronounced selectivity, showing lower cytotoxicity against normal fibroblasts MRC-5 compared to cancer cells. The effects of compounds 5b, 5e, and 5j on the cell cycle were investigated by flow cytometric analysis. It was found that these compounds cause the accumulation of cells in the subG1 and G1 phases of the cell cycle and induce caspase-dependent apoptosis, while the anti-angiogenic effects of 5b, 5e, and 5j have been confirmed in EA.hy926 cells using a tube formation assay. Further, the interaction of Bax protein with compound 5b was investigated by means of molecular modeling, applying the combined molecular docking/molecular dynamics approach.
T2  - Chemical Biology & Drug Design
T1  - Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies
IS  - 6
VL  - 89
DO  - 10.1111/cbdd.12920
SP  - 943
EP  - 952
ER  - 

@article{
author = "Milošev, Milorad Z and Jakovljević, Katarina and Joksović, Milan D and Stanojković, Tatjana and Matić, Ivana Z and Perović, Milka and Tešić, Vesna and Kanazir, Selma and Mladenović, Milan and Rodić, Marko V and Leovac, Vukadin M and Trifunović, Snežana and Marković, Violeta",
year = "2017",
abstract = "A series of 18 novel N-Mannich bases derived from 5-adamantyl-1,2,4-triazole-3-thione was synthesized and characterized using NMR spectroscopy and X-ray diffraction technique. All derivatives were evaluated for their anticancer potential against four human cancer cell lines. Several tested compounds exerted good cytotoxic activities on K562 and HL-60 cell lines, along with pronounced selectivity, showing lower cytotoxicity against normal fibroblasts MRC-5 compared to cancer cells. The effects of compounds 5b, 5e, and 5j on the cell cycle were investigated by flow cytometric analysis. It was found that these compounds cause the accumulation of cells in the subG1 and G1 phases of the cell cycle and induce caspase-dependent apoptosis, while the anti-angiogenic effects of 5b, 5e, and 5j have been confirmed in EA.hy926 cells using a tube formation assay. Further, the interaction of Bax protein with compound 5b was investigated by means of molecular modeling, applying the combined molecular docking/molecular dynamics approach.",
journal = "Chemical Biology & Drug Design",
title = "Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies",
number = "6",
volume = "89",
doi = "10.1111/cbdd.12920",
pages = "943-952"
}

Milošev, M. Z., Jakovljević, K., Joksović, M. D., Stanojković, T., Matić, I. Z., Perović, M., Tešić, V., Kanazir, S., Mladenović, M., Rodić, M. V., Leovac, V. M., Trifunović, S.,& Marković, V.. (2017). Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies. in Chemical Biology & Drug Design, 89(6), 943-952.
https://doi.org/10.1111/cbdd.12920

Milošev MZ, Jakovljević K, Joksović MD, Stanojković T, Matić IZ, Perović M, Tešić V, Kanazir S, Mladenović M, Rodić MV, Leovac VM, Trifunović S, Marković V. Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies. in Chemical Biology & Drug Design. 2017;89(6):943-952.
doi:10.1111/cbdd.12920 .

Milošev, Milorad Z, Jakovljević, Katarina, Joksović, Milan D, Stanojković, Tatjana, Matić, Ivana Z, Perović, Milka, Tešić, Vesna, Kanazir, Selma, Mladenović, Milan, Rodić, Marko V, Leovac, Vukadin M, Trifunović, Snežana, Marković, Violeta, "Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies" in Chemical Biology & Drug Design, 89, no. 6 (2017):943-952,
https://doi.org/10.1111/cbdd.12920 . .

TY  - JOUR
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
PY  - 2016
UR  - http://www.sciencedirect.com/science/article/pii/S1319562X15001138
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1319562X15001138
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3177
AB  - The Anacardiaceae Lindl. family comprises of many species which are used in nutrition and in traditional folk medicine for the treatment of several human diseases. Cotinus coggygria Scop. commonly known as “smoke tree”, is an commercial ornamental plant with high medicinal usages, belongs to the family Anacardiaceae. The present review provides a comprehensive report of empirical investigations on important pharmacological activities and phytochemical screening of essential oils and extracts. Relevant information was collected from scientific journals, books, and reports via library and electronic search using Medline, Pubmed, Google Scholar, ScienceDirect, Web of Science, and Scopus. The plant has been extensively investigated in a broad range of studies to provide scientific evidence for folklore claims or to find new therapeutic uses. Numerous activities namely antioxidative, antibacterial, antifungal, antiviral, anticancer, antigenotoxic, hepatoprotective and anti-inflammatory have been demonstrated for all parts of these plants by in vivo and in vitro studies. Essential oils and extracts showed various pharmacological and biological properties which make them an effective remedy for various kinds of illnesses. Considering data from the literature, it could be demonstrated that C. coggygria possesses diverse bioactive properties and immense utilization in medicine, health care, cosmetics and as health supplements.
T2  - Saudi Journal of Biological Sciences
T1  - Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential
IS  - 4
VL  - 23
DO  - 10.1016/j.sjbs.2015.05.012
SP  - 452
EP  - 461
ER  - 

@article{
author = "Matić, Sanja and Stanić, Snežana and Mihailović, Mirjana and Bogojević, Desanka",
year = "2016",
abstract = "The Anacardiaceae Lindl. family comprises of many species which are used in nutrition and in traditional folk medicine for the treatment of several human diseases. Cotinus coggygria Scop. commonly known as “smoke tree”, is an commercial ornamental plant with high medicinal usages, belongs to the family Anacardiaceae. The present review provides a comprehensive report of empirical investigations on important pharmacological activities and phytochemical screening of essential oils and extracts. Relevant information was collected from scientific journals, books, and reports via library and electronic search using Medline, Pubmed, Google Scholar, ScienceDirect, Web of Science, and Scopus. The plant has been extensively investigated in a broad range of studies to provide scientific evidence for folklore claims or to find new therapeutic uses. Numerous activities namely antioxidative, antibacterial, antifungal, antiviral, anticancer, antigenotoxic, hepatoprotective and anti-inflammatory have been demonstrated for all parts of these plants by in vivo and in vitro studies. Essential oils and extracts showed various pharmacological and biological properties which make them an effective remedy for various kinds of illnesses. Considering data from the literature, it could be demonstrated that C. coggygria possesses diverse bioactive properties and immense utilization in medicine, health care, cosmetics and as health supplements.",
journal = "Saudi Journal of Biological Sciences",
title = "Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential",
number = "4",
volume = "23",
doi = "10.1016/j.sjbs.2015.05.012",
pages = "452-461"
}

Matić, S., Stanić, S., Mihailović, M.,& Bogojević, D.. (2016). Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential. in Saudi Journal of Biological Sciences, 23(4), 452-461.
https://doi.org/10.1016/j.sjbs.2015.05.012

Matić S, Stanić S, Mihailović M, Bogojević D. Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential. in Saudi Journal of Biological Sciences. 2016;23(4):452-461.
doi:10.1016/j.sjbs.2015.05.012 .

Matić, Sanja, Stanić, Snežana, Mihailović, Mirjana, Bogojević, Desanka, "Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential" in Saudi Journal of Biological Sciences, 23, no. 4 (2016):452-461,
https://doi.org/10.1016/j.sjbs.2015.05.012 . .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Boroja, Tatjana
AU  - Vuković, Nenad
PY  - 2015
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84943457635&partnerID=tZOtx3y1
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0006295215005511
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3175
AB  - Eight chroman-2,4-diones, namely 2a-h, previously investigated as anticoagulants, of which 2a and 2f as the most active, were evaluated as in vivo genotoxic agents in Wistar rat livers and kidneys using the comet assay. Compounds 2a, 2b, and 2f without genotoxic activity were applied prior to ethyl methanesulfonate (EMS) and diminished EMS-induced DNA damage according to the total score and percentage of reduction. EMS produce harmful O(6)-ethylguanine lesion which is incorporated in aberrant genotoxic GT and TG pairing after ATP-dependent DNA strand breaks have been catalyzed by rat Topoisomerase IIα (rTopIIα, EC 5.99.1.3). Therefore, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on the enzyme level using molecular docking and molecular dynamics simulations insamuch as it had been determined that compounds do not intercalate DNA but instead inhibit the ATPase activity. Calculations predicted that compounds inhibit ATP hydrolysis before the DNA-EMS cleavage is being catalyzed by rTopIIα, prevent EMS mutagenic and carcinogenic effects, and beside anticoagulant activity can even be applied in the cancer treatment to control the rate of anticancer alkylation drugs.
PB  - Elsevier
T2  - Biochemical Pharmacology
T1  - Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach
IS  - 1
VL  - 98
DO  - 10.1016/j.bcp.2015.08.106
SP  - 243
EP  - 266
ER  - 

@article{
author = "Mladenović, Milan and Stanković, Nevena and Matić, Sanja and Stanić, Snežana and Mihailović, Mirjana and Mihailović, Vladimir and Katanić, Jelena and Boroja, Tatjana and Vuković, Nenad",
year = "2015",
abstract = "Eight chroman-2,4-diones, namely 2a-h, previously investigated as anticoagulants, of which 2a and 2f as the most active, were evaluated as in vivo genotoxic agents in Wistar rat livers and kidneys using the comet assay. Compounds 2a, 2b, and 2f without genotoxic activity were applied prior to ethyl methanesulfonate (EMS) and diminished EMS-induced DNA damage according to the total score and percentage of reduction. EMS produce harmful O(6)-ethylguanine lesion which is incorporated in aberrant genotoxic GT and TG pairing after ATP-dependent DNA strand breaks have been catalyzed by rat Topoisomerase IIα (rTopIIα, EC 5.99.1.3). Therefore, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on the enzyme level using molecular docking and molecular dynamics simulations insamuch as it had been determined that compounds do not intercalate DNA but instead inhibit the ATPase activity. Calculations predicted that compounds inhibit ATP hydrolysis before the DNA-EMS cleavage is being catalyzed by rTopIIα, prevent EMS mutagenic and carcinogenic effects, and beside anticoagulant activity can even be applied in the cancer treatment to control the rate of anticancer alkylation drugs.",
publisher = "Elsevier",
journal = "Biochemical Pharmacology",
title = "Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach",
number = "1",
volume = "98",
doi = "10.1016/j.bcp.2015.08.106",
pages = "243-266"
}

Mladenović, M., Stanković, N., Matić, S., Stanić, S., Mihailović, M., Mihailović, V., Katanić, J., Boroja, T.,& Vuković, N.. (2015). Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach. in Biochemical Pharmacology
Elsevier., 98(1), 243-266.
https://doi.org/10.1016/j.bcp.2015.08.106

Mladenović M, Stanković N, Matić S, Stanić S, Mihailović M, Mihailović V, Katanić J, Boroja T, Vuković N. Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach. in Biochemical Pharmacology. 2015;98(1):243-266.
doi:10.1016/j.bcp.2015.08.106 .

Mladenović, Milan, Stanković, Nevena, Matić, Sanja, Stanić, Snežana, Mihailović, Mirjana, Mihailović, Vladimir, Katanić, Jelena, Boroja, Tatjana, Vuković, Nenad, "Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach" in Biochemical Pharmacology, 98, no. 1 (2015):243-266,
https://doi.org/10.1016/j.bcp.2015.08.106 . .

TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Mišić, Danijela
AU  - Matić, Sanja
AU  - Mihailović, Mirjana
AU  - Stanić, Snežana
AU  - Vrvić, Miroslav M.
AU  - Katanić, Jelena
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Boroja, Tatjana
AU  - Stanković, Milan S.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2350
AB  - The purpose of this study was to evaluate the antioxidant potential of
   methanol extracts of Gentiana crudata L. aerial parts and roots, as well
   as the stability of the phenolic compounds and antioxidant capacity of
   extracts during heating, at different pHs and after an in vitro
   digestion procedure. Also, their genotoxicity and antigenotoxicity
   against carbon tetrachloride in the liver of albino Wistar rats using
   the comet assay were evaluated. Three secoiridoid glycosides
   (swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
   (orientin, vitexin and two isovitexin-glucosides) were identified as the
   major constituents in aerial parts and roots of G. cruciata, using
   UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
   showed that aerial parts displayed higher antioxidant activity compared
   to the roots, which could be related to higher phenolics content,
   especially flavonoids. In general, extracts showed pH and thermal
   stability, while duodenal condition had more influence on total phenolic
   condition and antioxidant activity of extracts. Both extracts showed a
   protective effect against CCl4 in comet assays. The roots extract showed
   no genotoxic activity, while aerial parts extract showed slight
   genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
   All rights reserved.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities
VL  - 73
DO  - 10.1016/j.indcrop.2015.04.013
SP  - 49
EP  - 62
ER  - 

@article{
author = "Mihailović, Vladimir and Mišić, Danijela and Matić, Sanja and Mihailović, Mirjana and Stanić, Snežana and Vrvić, Miroslav M. and Katanić, Jelena and Mladenović, Milan and Stanković, Nevena and Boroja, Tatjana and Stanković, Milan S.",
year = "2015",
abstract = "The purpose of this study was to evaluate the antioxidant potential of
   methanol extracts of Gentiana crudata L. aerial parts and roots, as well
   as the stability of the phenolic compounds and antioxidant capacity of
   extracts during heating, at different pHs and after an in vitro
   digestion procedure. Also, their genotoxicity and antigenotoxicity
   against carbon tetrachloride in the liver of albino Wistar rats using
   the comet assay were evaluated. Three secoiridoid glycosides
   (swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
   (orientin, vitexin and two isovitexin-glucosides) were identified as the
   major constituents in aerial parts and roots of G. cruciata, using
   UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
   showed that aerial parts displayed higher antioxidant activity compared
   to the roots, which could be related to higher phenolics content,
   especially flavonoids. In general, extracts showed pH and thermal
   stability, while duodenal condition had more influence on total phenolic
   condition and antioxidant activity of extracts. Both extracts showed a
   protective effect against CCl4 in comet assays. The roots extract showed
   no genotoxic activity, while aerial parts extract showed slight
   genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
   All rights reserved.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities",
volume = "73",
doi = "10.1016/j.indcrop.2015.04.013",
pages = "49-62"
}

Mihailović, V., Mišić, D., Matić, S., Mihailović, M., Stanić, S., Vrvić, M. M., Katanić, J., Mladenović, M., Stanković, N., Boroja, T.,& Stanković, M. S.. (2015). Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities. in Industrial Crops and Products
Elsevier., 73, 49-62.
https://doi.org/10.1016/j.indcrop.2015.04.013

Mihailović V, Mišić D, Matić S, Mihailović M, Stanić S, Vrvić MM, Katanić J, Mladenović M, Stanković N, Boroja T, Stanković MS. Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities. in Industrial Crops and Products. 2015;73:49-62.
doi:10.1016/j.indcrop.2015.04.013 .

Mihailović, Vladimir, Mišić, Danijela, Matić, Sanja, Mihailović, Mirjana, Stanić, Snežana, Vrvić, Miroslav M., Katanić, Jelena, Mladenović, Milan, Stanković, Nevena, Boroja, Tatjana, Stanković, Milan S., "Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities" in Industrial Crops and Products, 73 (2015):49-62,
https://doi.org/10.1016/j.indcrop.2015.04.013 . .

TY  - JOUR
AU  - Katanić, Jelena
AU  - Mihailović, Vladimir
AU  - Matić, Sanja
AU  - Stanković, Vesna
AU  - Stanković, Nevena
AU  - Boroja, Tatjana
AU  - Mladenović, Milan
AU  - Stanić, Snezana
AU  - Kreft, Samo
AU  - Mihailović, Mirjana
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2352
UR  - http://www.sciencedirect.com/science/article/pii/S175646461500362X
AB  - The effects of the methanolic extracts of Filipendula hexapetala Gilib.
   aerial parts (FHA) and roots (FHR) against cisplatin induced kidney and
   liver injuries in rats were investigated as well as determination of
   genotoxicity and antigenotoxicity of the extracts. Treatment with FHA
   and FHR significantly decreased levels of urea, uric acid, serum
   transaminases, alkaline phosphatase and gamma-glutamyl transferase, and
   increased the content of total protein. In addition, treatment with the
   extracts significantly attenuated the cisplatin-induced oxidative stress
   in kidney and liver tissues by increasing catalase and superoxide
   dismutase activities and the content of reduced glutathione and
   decreasing the content of thiobarbituric acid reactive substances
   (TSARS). The histopathological studies confirmed the protective effects
   of the extracts against cisplatin-induced kidney and liver injuries. The
   extracts ameliorated cisplatin-induced genotoxicity. These results
   suggest that F. hexapetala extracts are effective nephro- and
   hepatoprotective agents, with potential to reduce oxidative stress and
   ameliorate cisplatin-induced nephro- and hepatotoxicity.
T2  - Journal of Functional Foods
T1  - The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin
IS  - Part A
VL  - 18
DO  - 10.1016/j.jff.2015.07.004
SP  - 198
EP  - 212
ER  - 

@article{
author = "Katanić, Jelena and Mihailović, Vladimir and Matić, Sanja and Stanković, Vesna and Stanković, Nevena and Boroja, Tatjana and Mladenović, Milan and Stanić, Snezana and Kreft, Samo and Mihailović, Mirjana",
year = "2015",
abstract = "The effects of the methanolic extracts of Filipendula hexapetala Gilib.
   aerial parts (FHA) and roots (FHR) against cisplatin induced kidney and
   liver injuries in rats were investigated as well as determination of
   genotoxicity and antigenotoxicity of the extracts. Treatment with FHA
   and FHR significantly decreased levels of urea, uric acid, serum
   transaminases, alkaline phosphatase and gamma-glutamyl transferase, and
   increased the content of total protein. In addition, treatment with the
   extracts significantly attenuated the cisplatin-induced oxidative stress
   in kidney and liver tissues by increasing catalase and superoxide
   dismutase activities and the content of reduced glutathione and
   decreasing the content of thiobarbituric acid reactive substances
   (TSARS). The histopathological studies confirmed the protective effects
   of the extracts against cisplatin-induced kidney and liver injuries. The
   extracts ameliorated cisplatin-induced genotoxicity. These results
   suggest that F. hexapetala extracts are effective nephro- and
   hepatoprotective agents, with potential to reduce oxidative stress and
   ameliorate cisplatin-induced nephro- and hepatotoxicity.",
journal = "Journal of Functional Foods",
title = "The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin",
number = "Part A",
volume = "18",
doi = "10.1016/j.jff.2015.07.004",
pages = "198-212"
}

Katanić, J., Mihailović, V., Matić, S., Stanković, V., Stanković, N., Boroja, T., Mladenović, M., Stanić, S., Kreft, S.,& Mihailović, M.. (2015). The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin. in Journal of Functional Foods, 18(Part A), 198-212.
https://doi.org/10.1016/j.jff.2015.07.004

Katanić J, Mihailović V, Matić S, Stanković V, Stanković N, Boroja T, Mladenović M, Stanić S, Kreft S, Mihailović M. The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin. in Journal of Functional Foods. 2015;18(Part A):198-212.
doi:10.1016/j.jff.2015.07.004 .

Katanić, Jelena, Mihailović, Vladimir, Matić, Sanja, Stanković, Vesna, Stanković, Nevena, Boroja, Tatjana, Mladenović, Milan, Stanić, Snezana, Kreft, Samo, Mihailović, Mirjana, "The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin" in Journal of Functional Foods, 18, no. Part A (2015):198-212,
https://doi.org/10.1016/j.jff.2015.07.004 . .

TY  - JOUR
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Uskoković, Aleksandra
AU  - Stanković, Vesna
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Matić, Sanja
AU  - Solujić, Slavica
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
PY  - 2014
UR  - http://www.ncbi.nlm.nih.gov/pubmed/24468630
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3185
AB  - Eight synthesized 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones were evaluated as in vivo anticoagulants by intraperitoneal application to adult male Wistar rats in order to examine their pharmacological potential, evaluate ther toxicity and propose the mechanism of action. Two of them, 2f and 2a, in concentration of 2mg/kg of body weight, presented remarkable activity (PT=130s; PT=90s) upon seven days of continuous application. The results of rat serum and liver biochemical screening, as well those of histopathological studies, proved the compounds to be non-toxic. Activity of the compounds was further examined on the molecular level. Here, molecular docking studies were performed to position the compounds in relation to the active site of VKORC1 and determine the bioactive conformations. Docking results suggested a non-covalent mode of action during which the proton transfer occurs from Cys135 SH towards 4-carbonyl group of anticoagulant. All crucial interactions for anticoagulant activity were confirmed in generated structure-based 3-D pharmacophore model, consisted of hydrogen bond acceptor and hydrophobic aromatic features, and quantified by a best correlation coefficient of 0.97.
PB  - Elsevier
T2  - European Journal of Pharmaceutical Sciences
T1  - Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.
IS  - 1
VL  - 55
DO  - 10.1016/j.ejps.2014.01.004
SP  - 20
EP  - 35
ER  - 

@article{
author = "Stanković, Nevena and Mladenović, Milan and Mihailović, Mirjana and Arambašić Jovanović, Jelena and Uskoković, Aleksandra and Stanković, Vesna and Mihailović, Vladimir and Katanić, Jelena and Matić, Sanja and Solujić, Slavica and Vuković, Nenad and Sukdolak, Slobodan",
year = "2014",
abstract = "Eight synthesized 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones were evaluated as in vivo anticoagulants by intraperitoneal application to adult male Wistar rats in order to examine their pharmacological potential, evaluate ther toxicity and propose the mechanism of action. Two of them, 2f and 2a, in concentration of 2mg/kg of body weight, presented remarkable activity (PT=130s; PT=90s) upon seven days of continuous application. The results of rat serum and liver biochemical screening, as well those of histopathological studies, proved the compounds to be non-toxic. Activity of the compounds was further examined on the molecular level. Here, molecular docking studies were performed to position the compounds in relation to the active site of VKORC1 and determine the bioactive conformations. Docking results suggested a non-covalent mode of action during which the proton transfer occurs from Cys135 SH towards 4-carbonyl group of anticoagulant. All crucial interactions for anticoagulant activity were confirmed in generated structure-based 3-D pharmacophore model, consisted of hydrogen bond acceptor and hydrophobic aromatic features, and quantified by a best correlation coefficient of 0.97.",
publisher = "Elsevier",
journal = "European Journal of Pharmaceutical Sciences",
title = "Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.",
number = "1",
volume = "55",
doi = "10.1016/j.ejps.2014.01.004",
pages = "20-35"
}

Stanković, N., Mladenović, M., Mihailović, M., Arambašić Jovanović, J., Uskoković, A., Stanković, V., Mihailović, V., Katanić, J., Matić, S., Solujić, S., Vuković, N.,& Sukdolak, S.. (2014). Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.. in European Journal of Pharmaceutical Sciences
Elsevier., 55(1), 20-35.
https://doi.org/10.1016/j.ejps.2014.01.004

Stanković N, Mladenović M, Mihailović M, Arambašić Jovanović J, Uskoković A, Stanković V, Mihailović V, Katanić J, Matić S, Solujić S, Vuković N, Sukdolak S. Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.. in European Journal of Pharmaceutical Sciences. 2014;55(1):20-35.
doi:10.1016/j.ejps.2014.01.004 .

Stanković, Nevena, Mladenović, Milan, Mihailović, Mirjana, Arambašić Jovanović, Jelena, Uskoković, Aleksandra, Stanković, Vesna, Mihailović, Vladimir, Katanić, Jelena, Matić, Sanja, Solujić, Slavica, Vuković, Nenad, Sukdolak, Slobodan, "Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model." in European Journal of Pharmaceutical Sciences, 55, no. 1 (2014):20-35,
https://doi.org/10.1016/j.ejps.2014.01.004 . .

TY  - JOUR
AU  - Ristic, Aleksandar J.
AU  - Sokic, Dragoslav
AU  - Bascarevic, Vladimir
AU  - Spasic, Snezana
AU  - Vojvodic, Nikola
AU  - Savic, Slobodan
AU  - Raicevic, Savo
AU  - Kovacevic, Masa
AU  - Savić, Danijela
AU  - Spasojevic, Ivan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2214
AB  - An altered metal and electrolyte profile has been implicated in the
   pathologic mechanisms of chronic epilepsy; however, no study has
   comprehensively measured hippocampal concentrations of these elements in
   patients with mesial temporal lobe epilepsy and hippocampal sclerosis
   (mTLE-HS). We therefore analyzed hippocampi of 24 patients with
   drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior
   temporal lobe resection and amygdalohippocampectomy and 17 hippocampi
   obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years),
   using inductively coupled plasma optical emission spectrometry
   (ICP-OES). Epileptic hippocampi showed significantly lower
   concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control
   {[}C]: 3.57 +/- 0.33; p<0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409
   +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61;
   p<0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/-
   25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ
   in HS and controls. In summary, copper and manganese levels are
   deficient, whereas iron level is unchanged in hippocampi from patients
   with mTLE-HS. Our results provide a basis for understanding the
   potential involvement of different metals and electrolytes in the
   pathology of HS. A PowerPoint slide summarizing this article is
   available for download in the Supporting Information section .
T2  - Epilepsia
T1  - Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy
IS  - 5
VL  - 55
DO  - 10.1111/epi.12593
SP  - E34
EP  - E37
ER  - 

@article{
author = "Ristic, Aleksandar J. and Sokic, Dragoslav and Bascarevic, Vladimir and Spasic, Snezana and Vojvodic, Nikola and Savic, Slobodan and Raicevic, Savo and Kovacevic, Masa and Savić, Danijela and Spasojevic, Ivan",
year = "2014",
abstract = "An altered metal and electrolyte profile has been implicated in the
   pathologic mechanisms of chronic epilepsy; however, no study has
   comprehensively measured hippocampal concentrations of these elements in
   patients with mesial temporal lobe epilepsy and hippocampal sclerosis
   (mTLE-HS). We therefore analyzed hippocampi of 24 patients with
   drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior
   temporal lobe resection and amygdalohippocampectomy and 17 hippocampi
   obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years),
   using inductively coupled plasma optical emission spectrometry
   (ICP-OES). Epileptic hippocampi showed significantly lower
   concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control
   {[}C]: 3.57 +/- 0.33; p<0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409
   +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61;
   p<0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/-
   25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ
   in HS and controls. In summary, copper and manganese levels are
   deficient, whereas iron level is unchanged in hippocampi from patients
   with mTLE-HS. Our results provide a basis for understanding the
   potential involvement of different metals and electrolytes in the
   pathology of HS. A PowerPoint slide summarizing this article is
   available for download in the Supporting Information section .",
journal = "Epilepsia",
title = "Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy",
number = "5",
volume = "55",
doi = "10.1111/epi.12593",
pages = "E34-E37"
}

Ristic, A. J., Sokic, D., Bascarevic, V., Spasic, S., Vojvodic, N., Savic, S., Raicevic, S., Kovacevic, M., Savić, D.,& Spasojevic, I.. (2014). Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy. in Epilepsia, 55(5), E34-E37.
https://doi.org/10.1111/epi.12593

Ristic AJ, Sokic D, Bascarevic V, Spasic S, Vojvodic N, Savic S, Raicevic S, Kovacevic M, Savić D, Spasojevic I. Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy. in Epilepsia. 2014;55(5):E34-E37.
doi:10.1111/epi.12593 .

Ristic, Aleksandar J., Sokic, Dragoslav, Bascarevic, Vladimir, Spasic, Snezana, Vojvodic, Nikola, Savic, Slobodan, Raicevic, Savo, Kovacevic, Masa, Savić, Danijela, Spasojevic, Ivan, "Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy" in Epilepsia, 55, no. 5 (2014):E34-E37,
https://doi.org/10.1111/epi.12593 . .

TY  - CONF
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Bogojević, Desanka
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6141
AB  - The plant Cotinus coggygria Scop. (family Anacardiaceae) is commonly used in folk medicine for the treat­ment of various illnesses, such as diarrhea, paradontosis, gastric and duodenal ulcers. Different parts of this plant have been subjected to pharmacological evaluation for their potential antihaemorragic, wound-healing, anti­inflammatory, and antimicrobial activity. The present study was undertaken to investigate the phenolic conten according to the Folin-Ciocalteu procedure, while the spectrophotometric method with aluminium chloride was used for the determination of total ftavonoids. The phenolic and flavonoid composition of extract were deter­mined by the HPLC method. Antioxidant activiy was quantitatively determined using a DPPH radical scaveng­ing assay. To examine the antigenotoxic potential using the comet assay, Wistar rats were treated with 100 mg/ kg body weight of pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The content of total phenols and ftavonoids was 3.78 mg of gallic acid/g of dry plant material and 8.29 mg of rutin/g of dry plant material, respectively. HPLC analysis showed that myricetin was the dominant compound (511.5 µg/g), while hydroxyl derivatives of cinnammic acids (chlorogenic, caffeic, coumaric, ferulic and rosmaric acid) were identified in the extract in varying amount. The neutralisation of DPPH radicals was up to 95%, while the maximum inhibition concentration is approximately 125 µg/ml. The dose of 500 mg/kg body weight of the extract, that display no genotoxic activity, and its main flavonoid compound myricetin, in equivalent amount as present in the extract, were applied intraperitoneally either 2 or 12 h prior to pyrogallol. As measured by the decrease in total score and tail moment, the DNA damage in liver was reduced by the extract and myricetin.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract
SP  - 90
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6141
ER  - 

@conference{
author = "Matić, Sanja and Stanić, Snežana and Bogojević, Desanka and Solujić, Slavica and Mladenović, Milan and Stanković, Nevena and Mihailović, Vladimir and Katanić, Jelena and Mihailović, Mirjana",
year = "2013",
abstract = "The plant Cotinus coggygria Scop. (family Anacardiaceae) is commonly used in folk medicine for the treat­ment of various illnesses, such as diarrhea, paradontosis, gastric and duodenal ulcers. Different parts of this plant have been subjected to pharmacological evaluation for their potential antihaemorragic, wound-healing, anti­inflammatory, and antimicrobial activity. The present study was undertaken to investigate the phenolic conten according to the Folin-Ciocalteu procedure, while the spectrophotometric method with aluminium chloride was used for the determination of total ftavonoids. The phenolic and flavonoid composition of extract were deter­mined by the HPLC method. Antioxidant activiy was quantitatively determined using a DPPH radical scaveng­ing assay. To examine the antigenotoxic potential using the comet assay, Wistar rats were treated with 100 mg/ kg body weight of pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The content of total phenols and ftavonoids was 3.78 mg of gallic acid/g of dry plant material and 8.29 mg of rutin/g of dry plant material, respectively. HPLC analysis showed that myricetin was the dominant compound (511.5 µg/g), while hydroxyl derivatives of cinnammic acids (chlorogenic, caffeic, coumaric, ferulic and rosmaric acid) were identified in the extract in varying amount. The neutralisation of DPPH radicals was up to 95%, while the maximum inhibition concentration is approximately 125 µg/ml. The dose of 500 mg/kg body weight of the extract, that display no genotoxic activity, and its main flavonoid compound myricetin, in equivalent amount as present in the extract, were applied intraperitoneally either 2 or 12 h prior to pyrogallol. As measured by the decrease in total score and tail moment, the DNA damage in liver was reduced by the extract and myricetin.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract",
pages = "90-91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6141"
}

Matić, S., Stanić, S., Bogojević, D., Solujić, S., Mladenović, M., Stanković, N., Mihailović, V., Katanić, J.,& Mihailović, M.. (2013). Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 90-91.
https://hdl.handle.net/21.15107/rcub_ibiss_6141

Matić S, Stanić S, Bogojević D, Solujić S, Mladenović M, Stanković N, Mihailović V, Katanić J, Mihailović M. Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:90-91.
https://hdl.handle.net/21.15107/rcub_ibiss_6141 .

Matić, Sanja, Stanić, Snežana, Bogojević, Desanka, Solujić, Slavica, Mladenović, Milan, Stanković, Nevena, Mihailović, Vladimir, Katanić, Jelena, Mihailović, Mirjana, "Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):90-91,
https://hdl.handle.net/21.15107/rcub_ibiss_6141 .

TY  - CONF
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
AU  - Mišić, Danijela
AU  - Solujić, Slavica
AU  - Šipovac, Katarina
AU  - Stanković, Vesna
AU  - Mladenović, Milan
AU  - Stanković, Nevena
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6143
AB  - Gentiana plants are best known for their bitter taste that is due to the secoiridoids (e.g. swertiamarin, gen­tiopicrin, sweroside and amarogentin). Secoiridoid glucosides, gentiopicrin, swertiamarin and sweroside, are present in various traditional medicine preparations and are reported to have hepatoprotective activity. Many Gentiana species are known for their pharmaceutical values, such as Gentiana cruciata L., commonly called cross gentian. The dried roots and above-ground parts of G. cruciata are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing benefi­cial effects in gall and liver diseases. This study using in vivo model investigates hepatoprotective activity of the methanol extract of G. cruciata root (GCR) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCR (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days be­fore they were treated with CCl4 (1 ml/kg, 1 :1 mixture in olive oil) which caused liver injury. Pretreatment with GCR dose-dependently and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phos­phatase and total bilirubin, where as an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobar­bituric acid-reactive substances, as compared to CC14 treated group. Histological analyses also show that GCR reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macrove­sicular changes induced by CC14 in rats. The main secoiridoid compounds (sweroside, swertiamarin and gen­tiopicrin) present in GCR were identified and quantified to gain an insight into the compounds responsible for its hepatoprotective effects. The HPLC assay clearly indicated that GCR contained the greatest concentra­tion of gentiopicrin (5.45%), whereas concentration of sweroside (0.29%) and swertiamarin (0.09%) were lower.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract
SP  - 91
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6143
ER  - 

@conference{
author = "Mihailović, Vladimir and Katanić, Jelena and Mihailović, Mirjana and Mišić, Danijela and Solujić, Slavica and Šipovac, Katarina and Stanković, Vesna and Mladenović, Milan and Stanković, Nevena",
year = "2013",
abstract = "Gentiana plants are best known for their bitter taste that is due to the secoiridoids (e.g. swertiamarin, gen­tiopicrin, sweroside and amarogentin). Secoiridoid glucosides, gentiopicrin, swertiamarin and sweroside, are present in various traditional medicine preparations and are reported to have hepatoprotective activity. Many Gentiana species are known for their pharmaceutical values, such as Gentiana cruciata L., commonly called cross gentian. The dried roots and above-ground parts of G. cruciata are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing benefi­cial effects in gall and liver diseases. This study using in vivo model investigates hepatoprotective activity of the methanol extract of G. cruciata root (GCR) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCR (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days be­fore they were treated with CCl4 (1 ml/kg, 1 :1 mixture in olive oil) which caused liver injury. Pretreatment with GCR dose-dependently and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phos­phatase and total bilirubin, where as an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobar­bituric acid-reactive substances, as compared to CC14 treated group. Histological analyses also show that GCR reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macrove­sicular changes induced by CC14 in rats. The main secoiridoid compounds (sweroside, swertiamarin and gen­tiopicrin) present in GCR were identified and quantified to gain an insight into the compounds responsible for its hepatoprotective effects. The HPLC assay clearly indicated that GCR contained the greatest concentra­tion of gentiopicrin (5.45%), whereas concentration of sweroside (0.29%) and swertiamarin (0.09%) were lower.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract",
pages = "91-92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6143"
}

Mihailović, V., Katanić, J., Mihailović, M., Mišić, D., Solujić, S., Šipovac, K., Stanković, V., Mladenović, M.,& Stanković, N.. (2013). Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6143

Mihailović V, Katanić J, Mihailović M, Mišić D, Solujić S, Šipovac K, Stanković V, Mladenović M, Stanković N. Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6143 .

Mihailović, Vladimir, Katanić, Jelena, Mihailović, Mirjana, Mišić, Danijela, Solujić, Slavica, Šipovac, Katarina, Stanković, Vesna, Mladenović, Milan, Stanković, Nevena, "Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):91-92,
https://hdl.handle.net/21.15107/rcub_ibiss_6143 .

TY  - CONF
AU  - Mihailović, Vladimir
AU  - Mišić, Danijela
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
AU  - Solujić, Slavica
AU  - Stanković, Vesna
AU  - Mladenović, Milan
AU  - Stanković, Nevena
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6142
AB  - Many Gentiono species are known for their pharmaceutical values, such as Gentiono crucioto L, commonly called cross gentian [1]. The dried roots and above-ground parts of G. crucioto are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing beneficial effects in gall and liver diseases [2]. This study using in vivo model investigates hepatoprotective activity of G. crucioto aerial part methanol extract (GCA) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCA (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days before they were treated with CCl4 (1 ml/kg, 1:1 mixture in olive oil) which caused liver injury. Separation, determination and quantification of components in GCA was perform­ed using Dionex Ultimate 3000 UHPLC system equipped with a diode array detector (DAD) and connected to a triple-quadrupole mass spectrometer. Pretreatment with GCA dose-dependent­ly and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobarbituric acid-reactive substances, as compared to CCl4 treated group. Histological analyses also show that GCA reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macro-vesicular changes induced by CCl4 in rats. GCA was characterized by the presence of sweroside, swertiamarin, gentiopicrin, loganic acid, isovitexin 4',7-diglucoside, orientin and vitexin, as revealed by UHPLC-DAD-MS and UHPLC-MS/MS analyses. Quantification of targeted compounds in the SRM (selected reaction monitoring) experiment of UHPLC-MS/MS analysis clearly indicated that gentiopicrin (1.067%) was the dominant secoiridoid glycoside in GCA, whereas concentrations of sweroside (0.064%) and swertiamarin (0.033%) were significantly lower.
PB  - Belgrade: Serbian Chemical Society
C3  - Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia
T1  - Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats
SP  - 220
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6142
ER  - 

@conference{
author = "Mihailović, Vladimir and Mišić, Danijela and Katanić, Jelena and Mihailović, Mirjana and Solujić, Slavica and Stanković, Vesna and Mladenović, Milan and Stanković, Nevena",
year = "2013",
abstract = "Many Gentiono species are known for their pharmaceutical values, such as Gentiono crucioto L, commonly called cross gentian [1]. The dried roots and above-ground parts of G. crucioto are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing beneficial effects in gall and liver diseases [2]. This study using in vivo model investigates hepatoprotective activity of G. crucioto aerial part methanol extract (GCA) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCA (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days before they were treated with CCl4 (1 ml/kg, 1:1 mixture in olive oil) which caused liver injury. Separation, determination and quantification of components in GCA was perform­ed using Dionex Ultimate 3000 UHPLC system equipped with a diode array detector (DAD) and connected to a triple-quadrupole mass spectrometer. Pretreatment with GCA dose-dependent­ly and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobarbituric acid-reactive substances, as compared to CCl4 treated group. Histological analyses also show that GCA reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macro-vesicular changes induced by CCl4 in rats. GCA was characterized by the presence of sweroside, swertiamarin, gentiopicrin, loganic acid, isovitexin 4',7-diglucoside, orientin and vitexin, as revealed by UHPLC-DAD-MS and UHPLC-MS/MS analyses. Quantification of targeted compounds in the SRM (selected reaction monitoring) experiment of UHPLC-MS/MS analysis clearly indicated that gentiopicrin (1.067%) was the dominant secoiridoid glycoside in GCA, whereas concentrations of sweroside (0.064%) and swertiamarin (0.033%) were significantly lower.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia",
title = "Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats",
pages = "220",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6142"
}

Mihailović, V., Mišić, D., Katanić, J., Mihailović, M., Solujić, S., Stanković, V., Mladenović, M.,& Stanković, N.. (2013). Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats. in Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia
Belgrade: Serbian Chemical Society., 220.
https://hdl.handle.net/21.15107/rcub_ibiss_6142

Mihailović V, Mišić D, Katanić J, Mihailović M, Solujić S, Stanković V, Mladenović M, Stanković N. Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats. in Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia. 2013;:220.
https://hdl.handle.net/21.15107/rcub_ibiss_6142 .

Mihailović, Vladimir, Mišić, Danijela, Katanić, Jelena, Mihailović, Mirjana, Solujić, Slavica, Stanković, Vesna, Mladenović, Milan, Stanković, Nevena, "Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats" in Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia (2013):220,
https://hdl.handle.net/21.15107/rcub_ibiss_6142 .

TY  - JOUR
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Bogojević, Desanka
AU  - Vidaković, Melita
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Mihailović, Mirjana
PY  - 2013
UR  - http://www.sciencedirect.com/science/article/pii/S1383571813001836
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3188
AB  - The present study was undertaken to investigate the hepatoprotective effect of the methanol extract of Cotinus coggygria Scop. in rats exposed to the hepatotoxic compound pyrogallol. Assessed with the alkaline version of the comet assay, 1000 and 2000. mg/kg body weight (bw) of the extract showed a low level of genotoxicity, while 500. mg/kg bw of the extract showed no genotoxic potential. Quantitative HPLC analysis of phenolic acids and flavonoids in the methanol extract of C. coggygria showed that myricetin was a major component. To test the hepatoprotective effect, a non-genotoxic dose of the C. coggygria extract and an equivalent amount of synthetic myricetin, as present in the extract, were applied either 2 or 12. h prior to administration of 100. mg/kg bw of pyrogallol. The extract and myricetin promoted restoration of hepatic function by significantly reducing pyrogallol-induced elevation in the serum enzymes AST, ALT, ALP and in total bilirubin. As measured by the decrease in total score and tail moment, the DNA damage in liver was also reduced by the extract and by myricetin. Our results suggest that pro-surviving Akt activity and STAT3 protein expression play important roles in decreasing DNA damage and in mediating hepatic protection by the extract. These results suggest that myricetin, as a major component in the extract, is responsible for the antigenotoxic and hepatoprotective properties of the methanol extract of C. coggygria against pyrogallol-induced toxicity. © 2013 Elsevier B.V.
PB  - Elsevier
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury
IS  - 2
VL  - 755
DO  - 10.1016/j.mrgentox.2013.03.011
SP  - 81
EP  - 89
ER  - 

@article{
author = "Matić, Sanja and Stanić, Snežana and Bogojević, Desanka and Vidaković, Melita and Grdović, Nevena and Dinić, Svetlana and Solujić, Slavica and Mladenović, Milan and Stanković, Nevena and Mihailović, Mirjana",
year = "2013",
abstract = "The present study was undertaken to investigate the hepatoprotective effect of the methanol extract of Cotinus coggygria Scop. in rats exposed to the hepatotoxic compound pyrogallol. Assessed with the alkaline version of the comet assay, 1000 and 2000. mg/kg body weight (bw) of the extract showed a low level of genotoxicity, while 500. mg/kg bw of the extract showed no genotoxic potential. Quantitative HPLC analysis of phenolic acids and flavonoids in the methanol extract of C. coggygria showed that myricetin was a major component. To test the hepatoprotective effect, a non-genotoxic dose of the C. coggygria extract and an equivalent amount of synthetic myricetin, as present in the extract, were applied either 2 or 12. h prior to administration of 100. mg/kg bw of pyrogallol. The extract and myricetin promoted restoration of hepatic function by significantly reducing pyrogallol-induced elevation in the serum enzymes AST, ALT, ALP and in total bilirubin. As measured by the decrease in total score and tail moment, the DNA damage in liver was also reduced by the extract and by myricetin. Our results suggest that pro-surviving Akt activity and STAT3 protein expression play important roles in decreasing DNA damage and in mediating hepatic protection by the extract. These results suggest that myricetin, as a major component in the extract, is responsible for the antigenotoxic and hepatoprotective properties of the methanol extract of C. coggygria against pyrogallol-induced toxicity. © 2013 Elsevier B.V.",
publisher = "Elsevier",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury",
number = "2",
volume = "755",
doi = "10.1016/j.mrgentox.2013.03.011",
pages = "81-89"
}

Matić, S., Stanić, S., Bogojević, D., Vidaković, M., Grdović, N., Dinić, S., Solujić, S., Mladenović, M., Stanković, N.,& Mihailović, M.. (2013). Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier., 755(2), 81-89.
https://doi.org/10.1016/j.mrgentox.2013.03.011

Matić S, Stanić S, Bogojević D, Vidaković M, Grdović N, Dinić S, Solujić S, Mladenović M, Stanković N, Mihailović M. Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2013;755(2):81-89.
doi:10.1016/j.mrgentox.2013.03.011 .

Matić, Sanja, Stanić, Snežana, Bogojević, Desanka, Vidaković, Melita, Grdović, Nevena, Dinić, Svetlana, Solujić, Slavica, Mladenović, Milan, Stanković, Nevena, Mihailović, Mirjana, "Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 755, no. 2 (2013):81-89,
https://doi.org/10.1016/j.mrgentox.2013.03.011 . .

TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Mihailović, Mirjana
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Mišić, Danijela
AU  - Stanković, Vesna
AU  - Katanić, Jelena
AU  - Mladenović, Milan
AU  - Solujić, Slavica
AU  - Matić, Sanja
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1043
AB  - This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GM) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GM and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GM treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity. (C) 2012 Elsevier Ltd. All rights reserved.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats
VL  - 52
DO  - 10.1016/j.fct.2012.10.034
SP  - 83
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1043
ER  - 

@article{
author = "Mihailović, Vladimir and Mihailović, Mirjana and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Mišić, Danijela and Stanković, Vesna and Katanić, Jelena and Mladenović, Milan and Solujić, Slavica and Matić, Sanja",
year = "2013",
abstract = "This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GM) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GM and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GM treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity. (C) 2012 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats",
volume = "52",
doi = "10.1016/j.fct.2012.10.034",
pages = "83-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1043"
}

Mihailović, V., Mihailović, M., Uskoković, A., Arambašić Jovanović, J., Mišić, D., Stanković, V., Katanić, J., Mladenović, M., Solujić, S.,& Matić, S.. (2013). Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats. in Food and Chemical Toxicology
Elsevier., 52, 83-90.
https://doi.org/10.1016/j.fct.2012.10.034
https://hdl.handle.net/21.15107/rcub_ibiss_1043

Mihailović V, Mihailović M, Uskoković A, Arambašić Jovanović J, Mišić D, Stanković V, Katanić J, Mladenović M, Solujić S, Matić S. Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats. in Food and Chemical Toxicology. 2013;52:83-90.
doi:10.1016/j.fct.2012.10.034
https://hdl.handle.net/21.15107/rcub_ibiss_1043 .

Mihailović, Vladimir, Mihailović, Mirjana, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Mišić, Danijela, Stanković, Vesna, Katanić, Jelena, Mladenović, Milan, Solujić, Slavica, Matić, Sanja, "Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats" in Food and Chemical Toxicology, 52 (2013):83-90,
https://doi.org/10.1016/j.fct.2012.10.034 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1043 .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
AU  - Matić, Sanja
AU  - Nićiforović, Neda
AU  - Mihailović, Vladimir
AU  - Mašković, Pavle
AU  - Vrvić, Miroslav M.
AU  - Solujić, Slavica
PY  - 2012
UR  - https://www.sciencedirect.com/science/article/pii/S0223523412002887?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3201
AB  - The objective of this study was to investigate in vitro and in vivo anticoagulant activity of sixteen 4-hydroxycoumarin derivatives bearing polar C-3 scaffolds. The activity was evaluated by measuring prothrombin time. Enhanced anticoagulant activity in vitro was observed for all tested compounds. Upon successive administration of 0.5 mg/kg of body weight to adult Wistar rats, over a period of five days, four derivatives (2b, 4c, 5c and 9c) presented anticoagulant activity in vivo. The most active compound was 2b, with PT = 30.0 s. Low or non-toxic effects in vivo were determined based on the catalytic activity of liver enzymes and the concentration of bilirubin, iron and proteins. Metabolic pathways of the most active compounds in vivo were determined after GC/MS analysis of collected rat urine samples. The excretion occurs by glucuronidation of 7-hydroxy forms of tested derivatives. In vivo results were described using PLS-based CoMFA and CoMSIA 3D-QSAR studies, which showed CoMFA-SE (q2 = 0.738) and CoMSIA-SEA (q2 = 0.763) to be the statistically most relevant models. Furthermore, molecular docking and DFT mechanistic studies performed on the rat VKORC1 homology model revealed interactions between the 4-OH coumarin group in the form of phenolic anion and the Cys135 catalytic site in the transition state.
T2  - European Journal of Medicinal Chemistry
T1  - Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants
VL  - 54
DO  - 10.1016/J.EJMECH.2012.04.036
SP  - 144
EP  - 158
ER  - 

@article{
author = "Mladenović, Milan and Mihailović, Mirjana and Bogojević, Desanka and Vuković, Nenad and Sukdolak, Slobodan and Matić, Sanja and Nićiforović, Neda and Mihailović, Vladimir and Mašković, Pavle and Vrvić, Miroslav M. and Solujić, Slavica",
year = "2012",
abstract = "The objective of this study was to investigate in vitro and in vivo anticoagulant activity of sixteen 4-hydroxycoumarin derivatives bearing polar C-3 scaffolds. The activity was evaluated by measuring prothrombin time. Enhanced anticoagulant activity in vitro was observed for all tested compounds. Upon successive administration of 0.5 mg/kg of body weight to adult Wistar rats, over a period of five days, four derivatives (2b, 4c, 5c and 9c) presented anticoagulant activity in vivo. The most active compound was 2b, with PT = 30.0 s. Low or non-toxic effects in vivo were determined based on the catalytic activity of liver enzymes and the concentration of bilirubin, iron and proteins. Metabolic pathways of the most active compounds in vivo were determined after GC/MS analysis of collected rat urine samples. The excretion occurs by glucuronidation of 7-hydroxy forms of tested derivatives. In vivo results were described using PLS-based CoMFA and CoMSIA 3D-QSAR studies, which showed CoMFA-SE (q2 = 0.738) and CoMSIA-SEA (q2 = 0.763) to be the statistically most relevant models. Furthermore, molecular docking and DFT mechanistic studies performed on the rat VKORC1 homology model revealed interactions between the 4-OH coumarin group in the form of phenolic anion and the Cys135 catalytic site in the transition state.",
journal = "European Journal of Medicinal Chemistry",
title = "Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants",
volume = "54",
doi = "10.1016/J.EJMECH.2012.04.036",
pages = "144-158"
}

Mladenović, M., Mihailović, M., Bogojević, D., Vuković, N., Sukdolak, S., Matić, S., Nićiforović, N., Mihailović, V., Mašković, P., Vrvić, M. M.,& Solujić, S.. (2012). Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants. in European Journal of Medicinal Chemistry, 54, 144-158.
https://doi.org/10.1016/J.EJMECH.2012.04.036

Mladenović M, Mihailović M, Bogojević D, Vuković N, Sukdolak S, Matić S, Nićiforović N, Mihailović V, Mašković P, Vrvić MM, Solujić S. Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants. in European Journal of Medicinal Chemistry. 2012;54:144-158.
doi:10.1016/J.EJMECH.2012.04.036 .

Mladenović, Milan, Mihailović, Mirjana, Bogojević, Desanka, Vuković, Nenad, Sukdolak, Slobodan, Matić, Sanja, Nićiforović, Neda, Mihailović, Vladimir, Mašković, Pavle, Vrvić, Miroslav M., Solujić, Slavica, "Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants" in European Journal of Medicinal Chemistry, 54 (2012):144-158,
https://doi.org/10.1016/J.EJMECH.2012.04.036 . .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
AU  - Matić, Sanja
AU  - Nićiforović, Neda
AU  - Mihailović, Vladimir
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
AU  - Solujić, Slavica
PY  - 2011
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-79957795403&partnerID=tZOtx3y1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3207
AB  - The series of fifteen synthesized 4-hydroxycoumarin derivatives was subjected to antioxidant activity evaluation in vitro, through total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl radical, lipid peroxide scavenging and chelating activity. The highest activity was detected during the radicals scavenging, with 2b, 6b, 2c, and 4c noticed as the most active. The antioxidant activity was further quantified by the quantitative structure-activity relationships (QSAR) studies. For this purpose, the structures were optimized using Paramethric Method 6 (PM6) semi-empirical and Density Functional Theory (DFT) B3LYP methods. Bond dissociation enthalpies of coumarin 4-OH, Natural Bond Orbital (NBO) gained hybridization of the oxygen, acidity of the hydrogen atom and various molecular descriptors obtained, were correlated with biological activity, after which we designed 20 new antioxidant structures, using the most favorable structural motifs, with much improved predicted activity in vitro.
T2  - International Journal of Molecular Sciences
T1  - In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.
IS  - 5
VL  - 12
DO  - 10.3390/ijms12052822
SP  - 2822
EP  - 2841
ER  - 

@article{
author = "Mladenović, Milan and Mihailović, Mirjana and Bogojević, Desanka and Matić, Sanja and Nićiforović, Neda and Mihailović, Vladimir and Vuković, Nenad and Sukdolak, Slobodan and Solujić, Slavica",
year = "2011",
abstract = "The series of fifteen synthesized 4-hydroxycoumarin derivatives was subjected to antioxidant activity evaluation in vitro, through total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl radical, lipid peroxide scavenging and chelating activity. The highest activity was detected during the radicals scavenging, with 2b, 6b, 2c, and 4c noticed as the most active. The antioxidant activity was further quantified by the quantitative structure-activity relationships (QSAR) studies. For this purpose, the structures were optimized using Paramethric Method 6 (PM6) semi-empirical and Density Functional Theory (DFT) B3LYP methods. Bond dissociation enthalpies of coumarin 4-OH, Natural Bond Orbital (NBO) gained hybridization of the oxygen, acidity of the hydrogen atom and various molecular descriptors obtained, were correlated with biological activity, after which we designed 20 new antioxidant structures, using the most favorable structural motifs, with much improved predicted activity in vitro.",
journal = "International Journal of Molecular Sciences",
title = "In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.",
number = "5",
volume = "12",
doi = "10.3390/ijms12052822",
pages = "2822-2841"
}

Mladenović, M., Mihailović, M., Bogojević, D., Matić, S., Nićiforović, N., Mihailović, V., Vuković, N., Sukdolak, S.,& Solujić, S.. (2011). In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.. in International Journal of Molecular Sciences, 12(5), 2822-2841.
https://doi.org/10.3390/ijms12052822

Mladenović M, Mihailović M, Bogojević D, Matić S, Nićiforović N, Mihailović V, Vuković N, Sukdolak S, Solujić S. In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.. in International Journal of Molecular Sciences. 2011;12(5):2822-2841.
doi:10.3390/ijms12052822 .

Mladenović, Milan, Mihailović, Mirjana, Bogojević, Desanka, Matić, Sanja, Nićiforović, Neda, Mihailović, Vladimir, Vuković, Nenad, Sukdolak, Slobodan, Solujić, Slavica, "In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies." in International Journal of Molecular Sciences, 12, no. 5 (2011):2822-2841,
https://doi.org/10.3390/ijms12052822 . .