TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Anđelković, Marko
AU  - Tomović, Ljiljana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S1532045619300924?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3351
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3365
AB  - In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?
VL  - 223
DO  - 10.1016/J.CBPC.2019.05.010
SP  - 26
EP  - 34
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Anđelković, Marko and Tomović, Ljiljana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2019",
abstract = "In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?",
volume = "223",
doi = "10.1016/J.CBPC.2019.05.010",
pages = "26-34"
}

Gavrić, J., Despotović, S., Prokić, M., Gavrilović, B., Radovanović, T., Anđelković, M., Tomović, L., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2019). Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 223, 26-34.
https://doi.org/10.1016/J.CBPC.2019.05.010

Gavrić J, Despotović S, Prokić M, Gavrilović B, Radovanović T, Anđelković M, Tomović L, Borković Mitić S, Pavlović S, Saičić Z. Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019;223:26-34.
doi:10.1016/J.CBPC.2019.05.010 .

Gavrić, Jelena, Despotović, Svetlana, Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Anđelković, Marko, Tomović, Ljiljana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 223 (2019):26-34,
https://doi.org/10.1016/J.CBPC.2019.05.010 . .

TY  - JOUR
AU  - Matić, Miloš M.
AU  - Paunović, Milica G.
AU  - Milošević, Marija D.
AU  - Ognjanović, Branka I.
AU  - Saičić, Zorica
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/01480545.2019.1587451
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3309
AB  - Acetaminophen is widely used as an over-the-counter analgesic and antipyretic drug. The aim of the present study was to investigate the pro-oxidative effects of acetaminophen (300 mg/kg/day i.p.) and antioxidative effects of β-glucan (4 mg/kg/day i.p.) and/or vitamin C (100 mg/kg/day i.p.) on the blood parameters of treated rats. After 3 days of treatment, hematological and parameters of redox status were measured. Exposure of rats to acetaminophen caused significant changes in some hematological parameters and the glutathione redox cycle, leading to an increased concentration of oxidative stress parameters and the formation of lipid peroxidation, while the activities of antioxidant enzymes were decreased. Administration of β-glucan and/or vitamin C reduced lipid peroxidation and restored the levels of examined hematological and oxidative stress parameters and improved the activities of antioxidant enzymes. Obtained results demonstrated that acetaminophen has significant pro-oxidative effects and may disrupt redox balance in blood of rats, while the combination of β-glucan and/or vitamin C amplified the antioxidant defense potential and exhibited a strong hematoprotective activity against acetaminophen-induced toxicity. Therefore, β-glucan and vitamin C co-treatment may be a promising therapeutic option for the treatment of acute acetaminophen hematotoxicity.
T2  - Drug and Chemical Toxicology
T1  - Hematoprotective effects and antioxidant properties of β-glucan and vitamin C against acetaminophen-induced toxicity: an experimental study in rats.
DO  - 10.1080/01480545.2019.1587451
ER  - 

@article{
author = "Matić, Miloš M. and Paunović, Milica G. and Milošević, Marija D. and Ognjanović, Branka I. and Saičić, Zorica",
year = "2019",
abstract = "Acetaminophen is widely used as an over-the-counter analgesic and antipyretic drug. The aim of the present study was to investigate the pro-oxidative effects of acetaminophen (300 mg/kg/day i.p.) and antioxidative effects of β-glucan (4 mg/kg/day i.p.) and/or vitamin C (100 mg/kg/day i.p.) on the blood parameters of treated rats. After 3 days of treatment, hematological and parameters of redox status were measured. Exposure of rats to acetaminophen caused significant changes in some hematological parameters and the glutathione redox cycle, leading to an increased concentration of oxidative stress parameters and the formation of lipid peroxidation, while the activities of antioxidant enzymes were decreased. Administration of β-glucan and/or vitamin C reduced lipid peroxidation and restored the levels of examined hematological and oxidative stress parameters and improved the activities of antioxidant enzymes. Obtained results demonstrated that acetaminophen has significant pro-oxidative effects and may disrupt redox balance in blood of rats, while the combination of β-glucan and/or vitamin C amplified the antioxidant defense potential and exhibited a strong hematoprotective activity against acetaminophen-induced toxicity. Therefore, β-glucan and vitamin C co-treatment may be a promising therapeutic option for the treatment of acute acetaminophen hematotoxicity.",
journal = "Drug and Chemical Toxicology",
title = "Hematoprotective effects and antioxidant properties of β-glucan and vitamin C against acetaminophen-induced toxicity: an experimental study in rats.",
doi = "10.1080/01480545.2019.1587451"
}

Matić, M. M., Paunović, M. G., Milošević, M. D., Ognjanović, B. I.,& Saičić, Z.. (2019). Hematoprotective effects and antioxidant properties of β-glucan and vitamin C against acetaminophen-induced toxicity: an experimental study in rats.. in Drug and Chemical Toxicology.
https://doi.org/10.1080/01480545.2019.1587451

Matić MM, Paunović MG, Milošević MD, Ognjanović BI, Saičić Z. Hematoprotective effects and antioxidant properties of β-glucan and vitamin C against acetaminophen-induced toxicity: an experimental study in rats.. in Drug and Chemical Toxicology. 2019;.
doi:10.1080/01480545.2019.1587451 .

Matić, Miloš M., Paunović, Milica G., Milošević, Marija D., Ognjanović, Branka I., Saičić, Zorica, "Hematoprotective effects and antioxidant properties of β-glucan and vitamin C against acetaminophen-induced toxicity: an experimental study in rats." in Drug and Chemical Toxicology (2019),
https://doi.org/10.1080/01480545.2019.1587451 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Anđelković, Marko
AU  - Tomović, Ljiljana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S1532045619300924?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3351
AB  - In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?
DO  - 10.1016/J.CBPC.2019.05.010
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Anđelković, Marko and Tomović, Ljiljana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2019",
abstract = "In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?",
doi = "10.1016/J.CBPC.2019.05.010"
}

Gavrić, J., Despotović, S., Prokić, M., Gavrilović, B., Radovanović, T., Anđelković, M., Tomović, L., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2019). Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology.
https://doi.org/10.1016/J.CBPC.2019.05.010

Gavrić J, Despotović S, Prokić M, Gavrilović B, Radovanović T, Anđelković M, Tomović L, Borković Mitić S, Pavlović S, Saičić Z. Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019;.
doi:10.1016/J.CBPC.2019.05.010 .

Gavrić, Jelena, Despotović, Svetlana, Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Anđelković, Marko, Tomović, Ljiljana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology (2019),
https://doi.org/10.1016/J.CBPC.2019.05.010 . .

TY  - JOUR
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800045D
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3270
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3355
AB  - Metal pollution of the aquatic environment is of global concern because metals are ubiquitous and can be accumulated in natural habitats as well as in organisms through the food chain. Accumulated metals are capable of inducing toxicity in living organisms, altering their reproductive success, behavior, immune response and biochemical processes. We examined the correlation between the concentrations of 9 metals (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) in the whole body of the river snail Viviparus acerosus, river water and sediment from three Serbian rivers with different levels of metal pollution, the Danube, Tisa and Velika Morava. Data about water quality showed that the concentrations of As, Cr, Fe and Ni were highest in the water of the Danube and of Cu, Mn and Zn in the water of the Velika Morava River. The concentrations of As and Mn were highest in the Danube River sediment, of Cd, Cu, Fe, Pb and Zn in the Tisa and of Cr and Ni in the sediment of the Velika Morava. The concentrations of all of the examined metals, except for Cu, were highest in snails from the Velika Morava. Correspondence analysis showed stronger correlations between metal concentrations in snails and the river sediment than between snails and river water. Several correlations between metal concentrations in snails and river sediment and water were established by Pearson’s correlation test. The concentrations of metals in snail bodies were affected to a greater extent by the river sediment than by the river-water metal content. We conclude that V. acerosus has great potential as a bioindicator species of metal pollution in freshwater basins.
T2  - Archives of Biological Sciences
T1  - Evaluation of the river snail Viviparus acerosus as a potential bioindicator species of metal pollution in freshwater ecosystems
IS  - 1
VL  - 71
DO  - 10.2298/ABS180801045D
SP  - 39
EP  - 47
ER  - 

@article{
author = "Despotović, Svetlana and Prokić, Marko and Gavrić, Jelena and Gavrilović, Branka and Radovanović, Tijana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2019",
abstract = "Metal pollution of the aquatic environment is of global concern because metals are ubiquitous and can be accumulated in natural habitats as well as in organisms through the food chain. Accumulated metals are capable of inducing toxicity in living organisms, altering their reproductive success, behavior, immune response and biochemical processes. We examined the correlation between the concentrations of 9 metals (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) in the whole body of the river snail Viviparus acerosus, river water and sediment from three Serbian rivers with different levels of metal pollution, the Danube, Tisa and Velika Morava. Data about water quality showed that the concentrations of As, Cr, Fe and Ni were highest in the water of the Danube and of Cu, Mn and Zn in the water of the Velika Morava River. The concentrations of As and Mn were highest in the Danube River sediment, of Cd, Cu, Fe, Pb and Zn in the Tisa and of Cr and Ni in the sediment of the Velika Morava. The concentrations of all of the examined metals, except for Cu, were highest in snails from the Velika Morava. Correspondence analysis showed stronger correlations between metal concentrations in snails and the river sediment than between snails and river water. Several correlations between metal concentrations in snails and river sediment and water were established by Pearson’s correlation test. The concentrations of metals in snail bodies were affected to a greater extent by the river sediment than by the river-water metal content. We conclude that V. acerosus has great potential as a bioindicator species of metal pollution in freshwater basins.",
journal = "Archives of Biological Sciences",
title = "Evaluation of the river snail Viviparus acerosus as a potential bioindicator species of metal pollution in freshwater ecosystems",
number = "1",
volume = "71",
doi = "10.2298/ABS180801045D",
pages = "39-47"
}

Despotović, S., Prokić, M., Gavrić, J., Gavrilović, B., Radovanović, T., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2019). Evaluation of the river snail Viviparus acerosus as a potential bioindicator species of metal pollution in freshwater ecosystems. in Archives of Biological Sciences, 71(1), 39-47.
https://doi.org/10.2298/ABS180801045D

Despotović S, Prokić M, Gavrić J, Gavrilović B, Radovanović T, Borković Mitić S, Pavlović S, Saičić Z. Evaluation of the river snail Viviparus acerosus as a potential bioindicator species of metal pollution in freshwater ecosystems. in Archives of Biological Sciences. 2019;71(1):39-47.
doi:10.2298/ABS180801045D .

Despotović, Svetlana, Prokić, Marko, Gavrić, Jelena, Gavrilović, Branka, Radovanović, Tijana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Evaluation of the river snail Viviparus acerosus as a potential bioindicator species of metal pollution in freshwater ecosystems" in Archives of Biological Sciences, 71, no. 1 (2019):39-47,
https://doi.org/10.2298/ABS180801045D . .

TY  - CONF
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Krizmanić, Imre
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2018
UR  - http://www.vbs.rs/scripts/cobiss?command=DISPLAY&base=99999&rid=267801356&fmt=11&lani=sc
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3424
AB  - Anurans represent an interesting model organism for investigating changes in the oxidative stress during life time, as they have unique life cycle among tetrapods (consisted of eggs, tadpoles, juveniles, subadults and adults) related with terrestrial and aquatic environment. During the early phases of the life cycle, anuran individuals undergo drastic changes in the course of the transition from aquatic to terrestrial life style. Those changes are followed by metabolically demanding processes underlying rapid growth and responses to developmental pressures. All those processes could be responsible for the increased production of reactive oxygen species (ROS) and possible oxidative stress. The aim of this study was to investigate changes in antioxidative system response (SOD, CAT, GSH-Px, GR, GST, GSH and SH groups) and levels of oxidative damage (TBARS - lipid peroxidation) in natural developing Pelophylax esculentus complex frogs. Frogs were caught from May to July in 2017 at Special Nature Reserve Deliblatska Peščara. We divided development in four stages with clear differences: feeding and free-swimming tadpoles (covering 25-31 Gosner stage - GS), tadpoles with developed hindlimbs (35-41 GS), tadpoles with both limbs (42-46 GS) and juvenile individuals. Our results revealed that individuals at earlier stages (25-31 GS) faced with higher oxidative damage (higher TBARS and lower SH groups) that was followed by higher GR, GSH-Px and GST in comparison to other stadiums. Increased growth of individuals in this stadium and exposure to environmental factors directly for the first time may result in higher ROS production and as a consequence higher antioxidative system response and oxidative damage.
PB  - Belgrade: Serbian Society for Mitochondrial and Free Radical Physiology
C3  - 4th Congress: Challenges in Redox Physiology: Book of Abstracts. Belgrade, Serbia; September 28-30, 2018
T1  - Oxidative stress in natural developing tadpoles of Pelophylax esculentus complex frog
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3424
ER  - 

@conference{
author = "Prokić, Marko and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Krizmanić, Imre and Pavlović, Slađan and Saičić, Zorica",
year = "2018",
abstract = "Anurans represent an interesting model organism for investigating changes in the oxidative stress during life time, as they have unique life cycle among tetrapods (consisted of eggs, tadpoles, juveniles, subadults and adults) related with terrestrial and aquatic environment. During the early phases of the life cycle, anuran individuals undergo drastic changes in the course of the transition from aquatic to terrestrial life style. Those changes are followed by metabolically demanding processes underlying rapid growth and responses to developmental pressures. All those processes could be responsible for the increased production of reactive oxygen species (ROS) and possible oxidative stress. The aim of this study was to investigate changes in antioxidative system response (SOD, CAT, GSH-Px, GR, GST, GSH and SH groups) and levels of oxidative damage (TBARS - lipid peroxidation) in natural developing Pelophylax esculentus complex frogs. Frogs were caught from May to July in 2017 at Special Nature Reserve Deliblatska Peščara. We divided development in four stages with clear differences: feeding and free-swimming tadpoles (covering 25-31 Gosner stage - GS), tadpoles with developed hindlimbs (35-41 GS), tadpoles with both limbs (42-46 GS) and juvenile individuals. Our results revealed that individuals at earlier stages (25-31 GS) faced with higher oxidative damage (higher TBARS and lower SH groups) that was followed by higher GR, GSH-Px and GST in comparison to other stadiums. Increased growth of individuals in this stadium and exposure to environmental factors directly for the first time may result in higher ROS production and as a consequence higher antioxidative system response and oxidative damage.",
publisher = "Belgrade: Serbian Society for Mitochondrial and Free Radical Physiology",
journal = "4th Congress: Challenges in Redox Physiology: Book of Abstracts. Belgrade, Serbia; September 28-30, 2018",
title = "Oxidative stress in natural developing tadpoles of Pelophylax esculentus complex frog",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3424"
}

Prokić, M., Gavrić, J., Petrović, T., Despotović, S., Gavrilović, B., Radovanović, T., Krizmanić, I., Pavlović, S.,& Saičić, Z.. (2018). Oxidative stress in natural developing tadpoles of Pelophylax esculentus complex frog. in 4th Congress: Challenges in Redox Physiology: Book of Abstracts. Belgrade, Serbia; September 28-30, 2018
Belgrade: Serbian Society for Mitochondrial and Free Radical Physiology., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_3424

Prokić M, Gavrić J, Petrović T, Despotović S, Gavrilović B, Radovanović T, Krizmanić I, Pavlović S, Saičić Z. Oxidative stress in natural developing tadpoles of Pelophylax esculentus complex frog. in 4th Congress: Challenges in Redox Physiology: Book of Abstracts. Belgrade, Serbia; September 28-30, 2018. 2018;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_3424 .

Prokić, Marko, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Krizmanić, Imre, Pavlović, Slađan, Saičić, Zorica, "Oxidative stress in natural developing tadpoles of Pelophylax esculentus complex frog" in 4th Congress: Challenges in Redox Physiology: Book of Abstracts. Belgrade, Serbia; September 28-30, 2018 (2018):73,
https://hdl.handle.net/21.15107/rcub_ibiss_3424 .

TY  - JOUR
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Prokić, Marko
AU  - Saičić, Zorica
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700059P
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3019
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2500
AB  - We investigated the integrated response of antioxidant defense enzymes (total superoxide dismutase (TotSOD), manganese-containing superoxide dismutase (MnSOD), copper-zinc-containing superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and phase II biotransformation enzyme, glutathione- S-transferase (GST)) in the liver and white muscle of females of European hake (Merluccius merluccius L.) from the Adriatic Sea (Montenegro) in winter and spring. The activity of GSH-Px in the liver was significantly increased, while GST activity was decreased in spring compared to the winter. In white muscle, the activities of TotSOD and CuZnSOD were increased, while the activities of MnSOD, CAT, GSH-Px, GR and GST were decreased in spring when compared to the matching values in winter. The activities of TotSOD and CuZnSOD in winter were markedly lower in the muscle than in the liver, while the activity of MnSOD in the muscle was higher when compared to the liver. Principal component analysis (PCA) revealed clear separation of the investigated antioxidant biomarkers between tissues and seasons, while the integrated biomarker response (IBR) showed that the most intensive antioxidant biomarker response was in the liver in spring. Star plots of IBR showed a dominant contribution of glutathione-dependent biomarkers (GSH-Px, GR and GST) and CAT in both tissues and seasons with respect to SOD isoenzymes. All enzyme activities (except MnSOD) were greater in the liver in comparison to the white muscle. Our results show that the liver possesses a greater capacity to establish and maintain homeostasis under changing environmental conditions in winter and spring. At the same time, seasonal effects are more pronounced in muscle tissue
T2  - Archives of Biological Sciences
T1  - Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences
IS  - 1
VL  - 70
DO  - 10.2298/ABS171009059P
SP  - 205
EP  - 214
ER  - 

@article{
author = "Pavlović, Slađan and Borković Mitić, Slavica and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Prokić, Marko and Saičić, Zorica",
year = "2018",
abstract = "We investigated the integrated response of antioxidant defense enzymes (total superoxide dismutase (TotSOD), manganese-containing superoxide dismutase (MnSOD), copper-zinc-containing superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and phase II biotransformation enzyme, glutathione- S-transferase (GST)) in the liver and white muscle of females of European hake (Merluccius merluccius L.) from the Adriatic Sea (Montenegro) in winter and spring. The activity of GSH-Px in the liver was significantly increased, while GST activity was decreased in spring compared to the winter. In white muscle, the activities of TotSOD and CuZnSOD were increased, while the activities of MnSOD, CAT, GSH-Px, GR and GST were decreased in spring when compared to the matching values in winter. The activities of TotSOD and CuZnSOD in winter were markedly lower in the muscle than in the liver, while the activity of MnSOD in the muscle was higher when compared to the liver. Principal component analysis (PCA) revealed clear separation of the investigated antioxidant biomarkers between tissues and seasons, while the integrated biomarker response (IBR) showed that the most intensive antioxidant biomarker response was in the liver in spring. Star plots of IBR showed a dominant contribution of glutathione-dependent biomarkers (GSH-Px, GR and GST) and CAT in both tissues and seasons with respect to SOD isoenzymes. All enzyme activities (except MnSOD) were greater in the liver in comparison to the white muscle. Our results show that the liver possesses a greater capacity to establish and maintain homeostasis under changing environmental conditions in winter and spring. At the same time, seasonal effects are more pronounced in muscle tissue",
journal = "Archives of Biological Sciences",
title = "Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences",
number = "1",
volume = "70",
doi = "10.2298/ABS171009059P",
pages = "205-214"
}

Pavlović, S., Borković Mitić, S., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Prokić, M.,& Saičić, Z.. (2018). Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. in Archives of Biological Sciences, 70(1), 205-214.
https://doi.org/10.2298/ABS171009059P

Pavlović S, Borković Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. in Archives of Biological Sciences. 2018;70(1):205-214.
doi:10.2298/ABS171009059P .

Pavlović, Slađan, Borković Mitić, Slavica, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Prokić, Marko, Saičić, Zorica, "Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences" in Archives of Biological Sciences, 70, no. 1 (2018):205-214,
https://doi.org/10.2298/ABS171009059P . .

TY  - DATA
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Prokić, Marko
AU  - Saičić, Zorica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3716
AB  - Fig. S1. The geographical position of the investigated locality (Platamuni) in the Adriatic Sea.
T2  - Archives of Biological Sciences
T1  - Supplementary data for the article: Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. Arch Biol Sci. 2018;70(1):205–14.
IS  - 1
VL  - 70
SP  - 205
EP  - 214
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3716
ER  - 

@misc{
author = "Pavlović, Slađan and Borković Mitić, Slavica and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Prokić, Marko and Saičić, Zorica",
year = "2018",
abstract = "Fig. S1. The geographical position of the investigated locality (Platamuni) in the Adriatic Sea.",
journal = "Archives of Biological Sciences",
title = "Supplementary data for the article: Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. Arch Biol Sci. 2018;70(1):205–14.",
number = "1",
volume = "70",
pages = "205-214",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3716"
}

Pavlović, S., Borković Mitić, S., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Prokić, M.,& Saičić, Z.. (2018). Supplementary data for the article: Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. Arch Biol Sci. 2018;70(1):205–14.. in Archives of Biological Sciences, 70(1), 205-214.
https://hdl.handle.net/21.15107/rcub_ibiss_3716

Pavlović S, Borković Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Supplementary data for the article: Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. Arch Biol Sci. 2018;70(1):205–14.. in Archives of Biological Sciences. 2018;70(1):205-214.
https://hdl.handle.net/21.15107/rcub_ibiss_3716 .

Pavlović, Slađan, Borković Mitić, Slavica, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Prokić, Marko, Saičić, Zorica, "Supplementary data for the article: Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. Arch Biol Sci. 2018;70(1):205–14." in Archives of Biological Sciences, 70, no. 1 (2018):205-214,
https://hdl.handle.net/21.15107/rcub_ibiss_3716 .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Faggio, Caterina
AU  - Saičić, Zorica
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0944200618300242?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3144
AB  - During their complex life cycle, anurans are faced with various physiological and biochemical demands that can result in increased free radical production. The antioxidative system (AOS), assumes a central role in protection from oxidative stress, and increased knowledge of its response would allow us to identify and quantify underlying costs of free radical production. In this study, we compared the patterns and levels of integration of the AOS during two life stages of Bufotes viridis toads from natural populations: young, fully developed, reproductively inactive subadults and reproductively active adults. All examined parameters (SOD, CAT, GSH-Px, GR, GST, GSH and SH groups) were measured in the liver, skin and muscle. The obtained results showed that: (1) subadult individuals possessed a more integrated AOS (a higher index of integration and number of significant correlations), based mostly on the coordinated action of SOD-CAT; (2) adult individuals used the GSH-Px/GSH system; (3) all examined tissues exhibited similar patterns of variation of AOS parameters; (4) significant correlations between the AOS and biometric parameters. Our results indicate that adult and subadult toads differed in the way they processed free radicals, implying that adults may be more susceptible to oxidative damage. The knowledge from this study will contribute to a better understanding of the AOS and how its activity is modulated in natural populations of anurans throughout their life.
T2  - Zoology
T1  - Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad
VL  - 130
DO  - 10.1016/J.ZOOL.2018.08.001
SP  - 30
EP  - 37
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Faggio, Caterina and Saičić, Zorica",
year = "2018",
abstract = "During their complex life cycle, anurans are faced with various physiological and biochemical demands that can result in increased free radical production. The antioxidative system (AOS), assumes a central role in protection from oxidative stress, and increased knowledge of its response would allow us to identify and quantify underlying costs of free radical production. In this study, we compared the patterns and levels of integration of the AOS during two life stages of Bufotes viridis toads from natural populations: young, fully developed, reproductively inactive subadults and reproductively active adults. All examined parameters (SOD, CAT, GSH-Px, GR, GST, GSH and SH groups) were measured in the liver, skin and muscle. The obtained results showed that: (1) subadult individuals possessed a more integrated AOS (a higher index of integration and number of significant correlations), based mostly on the coordinated action of SOD-CAT; (2) adult individuals used the GSH-Px/GSH system; (3) all examined tissues exhibited similar patterns of variation of AOS parameters; (4) significant correlations between the AOS and biometric parameters. Our results indicate that adult and subadult toads differed in the way they processed free radicals, implying that adults may be more susceptible to oxidative damage. The knowledge from this study will contribute to a better understanding of the AOS and how its activity is modulated in natural populations of anurans throughout their life.",
journal = "Zoology",
title = "Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad",
volume = "130",
doi = "10.1016/J.ZOOL.2018.08.001",
pages = "30-37"
}

Prokić, M., Petrović, T., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Faggio, C.,& Saičić, Z.. (2018). Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad. in Zoology, 130, 30-37.
https://doi.org/10.1016/J.ZOOL.2018.08.001

Prokić M, Petrović T, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Faggio C, Saičić Z. Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad. in Zoology. 2018;130:30-37.
doi:10.1016/J.ZOOL.2018.08.001 .

Prokić, Marko, Petrović, Tamara, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Faggio, Caterina, Saičić, Zorica, "Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad" in Zoology, 130 (2018):30-37,
https://doi.org/10.1016/J.ZOOL.2018.08.001 . .

TY  - JOUR
AU  - Nasia, Mohammed M.
AU  - Radovanović, Tijana
AU  - Krizmanić, Imre I.
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2018
UR  - http://link.springer.com/10.1007/s11356-018-2979-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3142
AB  - Pesticide-induced oxidative stress, as one of mechanism of toxicity, has been a focus of toxicological research. However, there is a lack of data for certain pesticides-oxidative stress effects especially on terrestrial amphibians. This study evaluates the prooxidative effects of orally administered insecticide deltamethrin (DM) in some tissues of the terrestrial toad Bufotes viridis. Toads were randomly divided and assigned to a control group and a test group that was orally exposed to the pesticide (5 mg/kg of body weight/daily) for 21 days. Animals were euthanized from each group on days 7, 14, and 21, and the liver, leg muscle, ventral skin, and gastrointestinal tissue (GIT) were dissected and used for analysis. From battery of investigated antioxidant components, superoxide dismutase (SOD) was the most differentiate parameter in all examined tissues. For the period of prolonged exposure to pesticide, antioxidative strategy of Bufotes viridis was based on SOD utilization in attempt to maintain the oxidative disbalance at acceptable level. The integrated biomarker response (IBR) as the measure of the overall biochemical response to DM exposure revealed that the group exposed for 21 days had the highest response. Our work has offered valuable data ensuring evidence that toads exposed to deltamethrin developed adaptive reactions that were tissue-specific and that DM-generated systemic toxicity was time-dependent. The present work showed that oxidative stress has significant role in pesticide-induced toxicity and contributes to better understanding of ecotoxicological risk in the terrestrial amphibians exposed to DM.
T2  - Environmental Science and Pollution Research International
T1  - Prooxidant effects of chronic exposure to deltamethrin in green toad Bufotes viridis.
IS  - 30
VL  - 25
DO  - 10.1007/s11356-018-2979-x
SP  - 30597
EP  - 30608
ER  - 

@article{
author = "Nasia, Mohammed M. and Radovanović, Tijana and Krizmanić, Imre I. and Prokić, Marko and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2018",
abstract = "Pesticide-induced oxidative stress, as one of mechanism of toxicity, has been a focus of toxicological research. However, there is a lack of data for certain pesticides-oxidative stress effects especially on terrestrial amphibians. This study evaluates the prooxidative effects of orally administered insecticide deltamethrin (DM) in some tissues of the terrestrial toad Bufotes viridis. Toads were randomly divided and assigned to a control group and a test group that was orally exposed to the pesticide (5 mg/kg of body weight/daily) for 21 days. Animals were euthanized from each group on days 7, 14, and 21, and the liver, leg muscle, ventral skin, and gastrointestinal tissue (GIT) were dissected and used for analysis. From battery of investigated antioxidant components, superoxide dismutase (SOD) was the most differentiate parameter in all examined tissues. For the period of prolonged exposure to pesticide, antioxidative strategy of Bufotes viridis was based on SOD utilization in attempt to maintain the oxidative disbalance at acceptable level. The integrated biomarker response (IBR) as the measure of the overall biochemical response to DM exposure revealed that the group exposed for 21 days had the highest response. Our work has offered valuable data ensuring evidence that toads exposed to deltamethrin developed adaptive reactions that were tissue-specific and that DM-generated systemic toxicity was time-dependent. The present work showed that oxidative stress has significant role in pesticide-induced toxicity and contributes to better understanding of ecotoxicological risk in the terrestrial amphibians exposed to DM.",
journal = "Environmental Science and Pollution Research International",
title = "Prooxidant effects of chronic exposure to deltamethrin in green toad Bufotes viridis.",
number = "30",
volume = "25",
doi = "10.1007/s11356-018-2979-x",
pages = "30597-30608"
}

Nasia, M. M., Radovanović, T., Krizmanić, I. I., Prokić, M., Gavrić, J., Despotović, S., Gavrilović, B., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2018). Prooxidant effects of chronic exposure to deltamethrin in green toad Bufotes viridis.. in Environmental Science and Pollution Research International, 25(30), 30597-30608.
https://doi.org/10.1007/s11356-018-2979-x

Nasia MM, Radovanović T, Krizmanić II, Prokić M, Gavrić J, Despotović S, Gavrilović B, Borković Mitić S, Pavlović S, Saičić Z. Prooxidant effects of chronic exposure to deltamethrin in green toad Bufotes viridis.. in Environmental Science and Pollution Research International. 2018;25(30):30597-30608.
doi:10.1007/s11356-018-2979-x .

Nasia, Mohammed M., Radovanović, Tijana, Krizmanić, Imre I., Prokić, Marko, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Prooxidant effects of chronic exposure to deltamethrin in green toad Bufotes viridis." in Environmental Science and Pollution Research International, 25, no. 30 (2018):30597-30608,
https://doi.org/10.1007/s11356-018-2979-x . .

TY  - CONF
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2018
UR  - http://www.vbs.rs/scripts/cobiss?command=DISPLAY&base=99999&rid=267655948&fmt=11&lani=sc
UR  - http://www.serbiosoc.org.rs/wp-content/uploads/2018/11/DRUGI-KONGRES-BIOLOGA-SRBIJE-knjiga-sazetaka.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3416
AB  - Niske sredinske temperature tokom zimskih meseci predstavljaju izazov za zmije i druge ektotermne organizme. Prelazak u aktivno stanje u proleće zahteva povećanu potrošnju kiseonika što je u direktnoj vezi sa povećanom produkcijom reaktivnih vrsta kiseonika. Zato je bitno ispitati antioksidacione zaštitne mehanizme koji omogućavaju organizmima kod kojih dolazi do naglih promena u oksigenaciji tkiva da iz ovakvih stanja izađu bez oštećenja. Cilj ove studije bio je određivanje aktivnosti enzima zaštite od oksidacionih oštećenja: ukupne superoksid-dismutaze (SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px), glutation-raduktaze (GR), enzima faze II biotransformacije glutation-S-transferaze (GST), koncentracije glutationa (GSH) i nivoa lipidnih peroksida (TBARS) u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije. Dobijeni rezultati su pokazali da posle hibernacije u bubregu ispitivane vrste dolazi do značajnog povećanja aktivnosti CAT kao i koncentracije GSH uz smanjenje aktivnosti GST. U masnom tkivu takođe je zabeleženo značajno povećanje aktivnosti SOD u proleće. U bubregu nisu zabeležene razlike u koncentraciji TBARS, dok je u masnom tkivu nivo lipidne peroksidacije značajno smanjen u periodu posle u odnosu na period pre hibernacije. Naši rezultati pokazuju da antioksidacioni zaštitni sistem N. natrix ima važnu ulogu u sprečavanju nastanka oksidacionih oštećenja tokom prelaska u aktivno stanje nakon perioda hibernacije.
PB  - Belgrade: Serbian Biological Society
C3  - Osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka / Drugi kongres biologa Srbije, Kladovo, Srbija 25-30.09.2018
T1  - Biomarkeri oksidacionog stresa u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3416
ER  - 

@conference{
author = "Gavrić, Jelena and Despotović, Svetlana and Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2018",
abstract = "Niske sredinske temperature tokom zimskih meseci predstavljaju izazov za zmije i druge ektotermne organizme. Prelazak u aktivno stanje u proleće zahteva povećanu potrošnju kiseonika što je u direktnoj vezi sa povećanom produkcijom reaktivnih vrsta kiseonika. Zato je bitno ispitati antioksidacione zaštitne mehanizme koji omogućavaju organizmima kod kojih dolazi do naglih promena u oksigenaciji tkiva da iz ovakvih stanja izađu bez oštećenja. Cilj ove studije bio je određivanje aktivnosti enzima zaštite od oksidacionih oštećenja: ukupne superoksid-dismutaze (SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px), glutation-raduktaze (GR), enzima faze II biotransformacije glutation-S-transferaze (GST), koncentracije glutationa (GSH) i nivoa lipidnih peroksida (TBARS) u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije. Dobijeni rezultati su pokazali da posle hibernacije u bubregu ispitivane vrste dolazi do značajnog povećanja aktivnosti CAT kao i koncentracije GSH uz smanjenje aktivnosti GST. U masnom tkivu takođe je zabeleženo značajno povećanje aktivnosti SOD u proleće. U bubregu nisu zabeležene razlike u koncentraciji TBARS, dok je u masnom tkivu nivo lipidne peroksidacije značajno smanjen u periodu posle u odnosu na period pre hibernacije. Naši rezultati pokazuju da antioksidacioni zaštitni sistem N. natrix ima važnu ulogu u sprečavanju nastanka oksidacionih oštećenja tokom prelaska u aktivno stanje nakon perioda hibernacije.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka / Drugi kongres biologa Srbije, Kladovo, Srbija 25-30.09.2018",
title = "Biomarkeri oksidacionog stresa u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3416"
}

Gavrić, J., Despotović, S., Prokić, M., Gavrilović, B., Radovanović, T., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2018). Biomarkeri oksidacionog stresa u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije. in Osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka / Drugi kongres biologa Srbije, Kladovo, Srbija 25-30.09.2018
Belgrade: Serbian Biological Society..
https://hdl.handle.net/21.15107/rcub_ibiss_3416

Gavrić J, Despotović S, Prokić M, Gavrilović B, Radovanović T, Borković Mitić S, Pavlović S, Saičić Z. Biomarkeri oksidacionog stresa u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije. in Osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka / Drugi kongres biologa Srbije, Kladovo, Srbija 25-30.09.2018. 2018;.
https://hdl.handle.net/21.15107/rcub_ibiss_3416 .

Gavrić, Jelena, Despotović, Svetlana, Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Biomarkeri oksidacionog stresa u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije" in Osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka / Drugi kongres biologa Srbije, Kladovo, Srbija 25-30.09.2018 (2018),
https://hdl.handle.net/21.15107/rcub_ibiss_3416 .

TY  - JOUR
AU  - Milošević, Marija D.
AU  - Paunović, Milica G.
AU  - Matić, Miloš M.
AU  - Ognjanović, Branka I.
AU  - Saičić, Zorica
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0753332218311636?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3100
AB  - Excessive use of organophosphate insecticides, including fenitrothion (FNT) can cause detrimental consequences in non-target organisms. Selenium (Se) and vitamin C (Vit C) possess protective abilities against various toxic compounds due to their antioxidative properties. Accordingly, the aim of the present study was to examine the possible ameliorative effects of Se and Vit C in hepatotoxicity induced by FNT. For the purpose of this study, male Wistar albino rats were divided into control and groups treated with Se (0.5 mg/kg b.w, as Na2SeO3) and Vit C (100 mg/kg b.w), FNT (20 mg/kg b.w) and FNT in cotreatment with Se and Vit C for 30 days. The current data showed a reduction in absolute and relative liver weight after FNT administration. Increased activities of liver enzymes (AST, ALT, ALP, LDH and GGT) indicated liver damage. FNT alone caused significant alterations in biochemical parameters (glucose and total bilirubin). Elevation in LPO level along with decreased activities of antioxidant enzymes (SOD, CAT, GSH-Px) and GSH content reflected the presence of oxidative stress. Coadministration of FNT with Se and Vit C exhibited hepatoprotective role confirmed by reduction of oxidative stress levels and restoration in the values of examined parameters. Because of their beneficial effects, Se and Vit C may be used in reducing injuries caused by pesticides.
T2  - Biomedicine & Pharmacotherapy
T1  - Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver.
VL  - 106
DO  - 10.1016/j.biopha.2018.06.132
SP  - 232
EP  - 238
ER  - 

@article{
author = "Milošević, Marija D. and Paunović, Milica G. and Matić, Miloš M. and Ognjanović, Branka I. and Saičić, Zorica",
year = "2018",
abstract = "Excessive use of organophosphate insecticides, including fenitrothion (FNT) can cause detrimental consequences in non-target organisms. Selenium (Se) and vitamin C (Vit C) possess protective abilities against various toxic compounds due to their antioxidative properties. Accordingly, the aim of the present study was to examine the possible ameliorative effects of Se and Vit C in hepatotoxicity induced by FNT. For the purpose of this study, male Wistar albino rats were divided into control and groups treated with Se (0.5 mg/kg b.w, as Na2SeO3) and Vit C (100 mg/kg b.w), FNT (20 mg/kg b.w) and FNT in cotreatment with Se and Vit C for 30 days. The current data showed a reduction in absolute and relative liver weight after FNT administration. Increased activities of liver enzymes (AST, ALT, ALP, LDH and GGT) indicated liver damage. FNT alone caused significant alterations in biochemical parameters (glucose and total bilirubin). Elevation in LPO level along with decreased activities of antioxidant enzymes (SOD, CAT, GSH-Px) and GSH content reflected the presence of oxidative stress. Coadministration of FNT with Se and Vit C exhibited hepatoprotective role confirmed by reduction of oxidative stress levels and restoration in the values of examined parameters. Because of their beneficial effects, Se and Vit C may be used in reducing injuries caused by pesticides.",
journal = "Biomedicine & Pharmacotherapy",
title = "Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver.",
volume = "106",
doi = "10.1016/j.biopha.2018.06.132",
pages = "232-238"
}

Milošević, M. D., Paunović, M. G., Matić, M. M., Ognjanović, B. I.,& Saičić, Z.. (2018). Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver.. in Biomedicine & Pharmacotherapy, 106, 232-238.
https://doi.org/10.1016/j.biopha.2018.06.132

Milošević MD, Paunović MG, Matić MM, Ognjanović BI, Saičić Z. Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver.. in Biomedicine & Pharmacotherapy. 2018;106:232-238.
doi:10.1016/j.biopha.2018.06.132 .

Milošević, Marija D., Paunović, Milica G., Matić, Miloš M., Ognjanović, Branka I., Saičić, Zorica, "Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver." in Biomedicine & Pharmacotherapy, 106 (2018):232-238,
https://doi.org/10.1016/j.biopha.2018.06.132 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Despotović, Svetlana
AU  - Vučić, Tijana
AU  - Petrović, Tamara
AU  - Gavrić, Jelena
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Saičić, Zorica
PY  - 2018
UR  - http://jeb.biologists.org/content/early/2018/08/17/jeb.182055
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3151
AB  - Oxidative stress has most recently been suggested as one of possible mechanisms responsible for reduced fitness of hybrids. To explore possible oxidative cost of hybridization, we examined antioxidant defense system parameters (superoxide dismutase, catalase, glutathione peroxidase, glutathione s-transferase, glutathione reductase, glutathione, SH groups), their interconnectedness (index of integration), and levels of oxidative damage (concentrations of lipid peroxides-TBARS) in lab-reared newt species, Triturus macedonicus and T. ivanbureschi, and their hybrid. Our results showed that parental species differed in antioxidant defense system parameters, but not in the levels of integration of the whole system and oxidative damage. Individuals of T. ivanbureschi had higher activities of superoxide dismutase, glutathione s-transferase and concentrations of glutathione. Hybrid individuals of crested newts displayed higher levels of the antioxidant defense system (higher superoxide dismutase, catalase, glutathione peroxidase activities and concentrations of SH groups), and a lower overall correlation of antioxidant system (lower index of integration) in comparison to both parental species, suggesting that they may possess a less efficient antioxidant defense system and a higher investment in maintaining oxidative balance. The higher investment in the antioxidant system could divert limited resources away from other functions and affect further hybrid fitness. The presented findings contribute to a better understanding of the antioxidant defense system of crested newts and their interspecies differences, and support the hypothesis that oxidative stress is one of the costs of interspecific hybridization.
T2  - The Journal of Experimental Biology
T1  - Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.
DO  - 10.1242/jeb.182055
ER  - 

@article{
author = "Prokić, Marko and Despotović, Svetlana and Vučić, Tijana and Petrović, Tamara and Gavrić, Jelena and Gavrilović, Branka and Radovanović, Tijana and Saičić, Zorica",
year = "2018",
abstract = "Oxidative stress has most recently been suggested as one of possible mechanisms responsible for reduced fitness of hybrids. To explore possible oxidative cost of hybridization, we examined antioxidant defense system parameters (superoxide dismutase, catalase, glutathione peroxidase, glutathione s-transferase, glutathione reductase, glutathione, SH groups), their interconnectedness (index of integration), and levels of oxidative damage (concentrations of lipid peroxides-TBARS) in lab-reared newt species, Triturus macedonicus and T. ivanbureschi, and their hybrid. Our results showed that parental species differed in antioxidant defense system parameters, but not in the levels of integration of the whole system and oxidative damage. Individuals of T. ivanbureschi had higher activities of superoxide dismutase, glutathione s-transferase and concentrations of glutathione. Hybrid individuals of crested newts displayed higher levels of the antioxidant defense system (higher superoxide dismutase, catalase, glutathione peroxidase activities and concentrations of SH groups), and a lower overall correlation of antioxidant system (lower index of integration) in comparison to both parental species, suggesting that they may possess a less efficient antioxidant defense system and a higher investment in maintaining oxidative balance. The higher investment in the antioxidant system could divert limited resources away from other functions and affect further hybrid fitness. The presented findings contribute to a better understanding of the antioxidant defense system of crested newts and their interspecies differences, and support the hypothesis that oxidative stress is one of the costs of interspecific hybridization.",
journal = "The Journal of Experimental Biology",
title = "Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.",
doi = "10.1242/jeb.182055"
}

Prokić, M., Despotović, S., Vučić, T., Petrović, T., Gavrić, J., Gavrilović, B., Radovanović, T.,& Saičić, Z.. (2018). Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.. in The Journal of Experimental Biology.
https://doi.org/10.1242/jeb.182055

Prokić M, Despotović S, Vučić T, Petrović T, Gavrić J, Gavrilović B, Radovanović T, Saičić Z. Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.. in The Journal of Experimental Biology. 2018;.
doi:10.1242/jeb.182055 .

Prokić, Marko, Despotović, Svetlana, Vučić, Tijana, Petrović, Tamara, Gavrić, Jelena, Gavrilović, Branka, Radovanović, Tijana, Saičić, Zorica, "Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids." in The Journal of Experimental Biology (2018),
https://doi.org/10.1242/jeb.182055 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Despotović, Svetlana
AU  - Vučić, Tijana
AU  - Petrović, Tamara
AU  - Gavrić, Jelena
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Saičić, Zorica
PY  - 2018
UR  - http://jeb.biologists.org/content/early/2018/08/17/jeb.182055
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3160
AB  - Oxidative stress has most recently been suggested as one of the possible mechanisms responsible for reduced fitness of hybrids. To explore possible oxidative cost of hybridization, we examined anti-oxidant defence system parameters (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, glutathione, SH groups), their interconnectedness (index of integration) and levels of oxidative damage [concentrations of lipid peroxides, TBARS (thiobarbituric acid reactive substances)] in laboratory-reared newt species, Triturus macedonicus and T. ivanbureschi, and their hybrid. Our results showed that parental species differed in anti-oxidant defence system parameters, but not in the levels of integration of the whole system and oxidative damage. Individuals of T. ivanbureschi had higher activities of superoxide dismutase, glutathione S-transferase and concentrations of glutathione. Hybrid individuals of crested newts displayed higher levels of the anti-oxidant defence system (higher superoxide dismutase, catalase, glutathione peroxidase activities and concentrations of SH groups), and a lower overall correlation of anti-oxidant system (lower index of integration) in comparison with both parental species, suggesting that they may possess a less efficient anti-oxidant defence system and a higher investment in maintaining oxidative balance. The higher investment in the anti-oxidant system could divert limited resources away from other functions and affect further hybrid fitness. The presented findings contribute to a better understanding of the anti-oxidant defence system of crested newts and their interspecies differences, and support the hypothesis that oxidative stress is one of the costs of interspecific hybridization.
T2  - The Journal of Experimental Biology
T1  - Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.
IS  - Pt 19
VL  - 221
DO  - 10.1242/jeb.182055
SP  - jeb182055
ER  - 

@article{
author = "Prokić, Marko and Despotović, Svetlana and Vučić, Tijana and Petrović, Tamara and Gavrić, Jelena and Gavrilović, Branka and Radovanović, Tijana and Saičić, Zorica",
year = "2018",
abstract = "Oxidative stress has most recently been suggested as one of the possible mechanisms responsible for reduced fitness of hybrids. To explore possible oxidative cost of hybridization, we examined anti-oxidant defence system parameters (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, glutathione, SH groups), their interconnectedness (index of integration) and levels of oxidative damage [concentrations of lipid peroxides, TBARS (thiobarbituric acid reactive substances)] in laboratory-reared newt species, Triturus macedonicus and T. ivanbureschi, and their hybrid. Our results showed that parental species differed in anti-oxidant defence system parameters, but not in the levels of integration of the whole system and oxidative damage. Individuals of T. ivanbureschi had higher activities of superoxide dismutase, glutathione S-transferase and concentrations of glutathione. Hybrid individuals of crested newts displayed higher levels of the anti-oxidant defence system (higher superoxide dismutase, catalase, glutathione peroxidase activities and concentrations of SH groups), and a lower overall correlation of anti-oxidant system (lower index of integration) in comparison with both parental species, suggesting that they may possess a less efficient anti-oxidant defence system and a higher investment in maintaining oxidative balance. The higher investment in the anti-oxidant system could divert limited resources away from other functions and affect further hybrid fitness. The presented findings contribute to a better understanding of the anti-oxidant defence system of crested newts and their interspecies differences, and support the hypothesis that oxidative stress is one of the costs of interspecific hybridization.",
journal = "The Journal of Experimental Biology",
title = "Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.",
number = "Pt 19",
volume = "221",
doi = "10.1242/jeb.182055",
pages = "jeb182055"
}

Prokić, M., Despotović, S., Vučić, T., Petrović, T., Gavrić, J., Gavrilović, B., Radovanović, T.,& Saičić, Z.. (2018). Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.. in The Journal of Experimental Biology, 221(Pt 19), jeb182055.
https://doi.org/10.1242/jeb.182055

Prokić M, Despotović S, Vučić T, Petrović T, Gavrić J, Gavrilović B, Radovanović T, Saičić Z. Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.. in The Journal of Experimental Biology. 2018;221(Pt 19):jeb182055.
doi:10.1242/jeb.182055 .

Prokić, Marko, Despotović, Svetlana, Vučić, Tijana, Petrović, Tamara, Gavrić, Jelena, Gavrilović, Branka, Radovanović, Tijana, Saičić, Zorica, "Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids." in The Journal of Experimental Biology, 221, no. Pt 19 (2018):jeb182055,
https://doi.org/10.1242/jeb.182055 . .

TY  - CONF
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Krizmanić, Imre
AU  - Gavrić, Jelena
AU  - Nasia, Mohammed
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Despotović, Svetlana
AU  - Radovanović, Tijana
AU  - Saičić, Zorica
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3420
AB  - In temperate regions, low temperatures and the lack of food during the
winter lead the water frogs to hibernate in order to survive. Hibernation
represents an adaptation mechanism and is a part of frogs yearly life cycle
(Wells, 2007). Hibernation, as well as pre- (autumn) and post-hibernation
(spring) periods are characterized by different biochemical, physiological,
morphological and behavioral changes, all in order to prepare the organisms
for a significant depression in aerobic metabolic rate (autumn) and then
recovery to a normal state (spring). An important role in this response plays
the antioxidative defense system (AOS), especially in spring, when increased
metabolic rate during arousal is followed by high oxygen consumption and
reactive oxygen species (ROS) generation. Oxidative stress is also induced by
various xenobiotics in the environment and this raises the question of their
share in oxidative stress of hibernators in a yearly cycle?
To examine this we first determined concentrations and seasonal
variations of accumulated metals (As, Cd, Cr, Fe, Hg, Ni, Pb, Zn) and AOS
parameters (superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GSH- Px), glutathione reductase (GR), glutathione-S-transferase
(GST) and glutathione (GSH) and sulfhydryl (SH) groups concentrations) in
skin and muscle of Pelophylax ridibundus during pre- (autumn) and post- hibernation (spring) periods, and second we evaluated the possible
relationship between accumulated metals and AOS parameters.
The results showed seasonal variation of accumulated concentrations:
autumn frogs were characterized by higher concentrations of Ni, Cr and Hg,
while frogs sampled in spring had higher As and Cd. On the other hand
almost all AOS parameters were higher during the spring in comparison to
individuals from autumn (in skin SOD, CAT, GR, GST, GSH and SH groups,
while in muscle SOD, CAT, GSH-Px, GR and SH groups). The number of
significant correlations between accumulated metals and AOS parameters
were very similar in frogs from both seasons.
All results showed that oxidative stress in the post-hibernating season was
mainly caused by the organism’s recovery from hibernation, and that the
metals did not contribute significantly to this response, indicating that natural
selection affected AOS of the frogs in that way that during arousal they were
able to produce higher levels of antioxidant defenses.
PB  - Association of Naturalists Students of the Jagiellonian University
C3  - Abstract Book: Conference of young evolutionary biologist; 2017 Jun 2-4; Krakow, Poland
T1  - Heavy metals, oxidative stress and their relationship in Pelophylax ridibundus frogs during pre- and posthibernation period
SP  - 45
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3420
ER  - 

@conference{
author = "Prokić, Marko and Gavrilović, Branka and Krizmanić, Imre and Gavrić, Jelena and Nasia, Mohammed and Borković Mitić, Slavica and Pavlović, Slađan and Despotović, Svetlana and Radovanović, Tijana and Saičić, Zorica",
year = "2017",
abstract = "In temperate regions, low temperatures and the lack of food during the
winter lead the water frogs to hibernate in order to survive. Hibernation
represents an adaptation mechanism and is a part of frogs yearly life cycle
(Wells, 2007). Hibernation, as well as pre- (autumn) and post-hibernation
(spring) periods are characterized by different biochemical, physiological,
morphological and behavioral changes, all in order to prepare the organisms
for a significant depression in aerobic metabolic rate (autumn) and then
recovery to a normal state (spring). An important role in this response plays
the antioxidative defense system (AOS), especially in spring, when increased
metabolic rate during arousal is followed by high oxygen consumption and
reactive oxygen species (ROS) generation. Oxidative stress is also induced by
various xenobiotics in the environment and this raises the question of their
share in oxidative stress of hibernators in a yearly cycle?
To examine this we first determined concentrations and seasonal
variations of accumulated metals (As, Cd, Cr, Fe, Hg, Ni, Pb, Zn) and AOS
parameters (superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GSH- Px), glutathione reductase (GR), glutathione-S-transferase
(GST) and glutathione (GSH) and sulfhydryl (SH) groups concentrations) in
skin and muscle of Pelophylax ridibundus during pre- (autumn) and post- hibernation (spring) periods, and second we evaluated the possible
relationship between accumulated metals and AOS parameters.
The results showed seasonal variation of accumulated concentrations:
autumn frogs were characterized by higher concentrations of Ni, Cr and Hg,
while frogs sampled in spring had higher As and Cd. On the other hand
almost all AOS parameters were higher during the spring in comparison to
individuals from autumn (in skin SOD, CAT, GR, GST, GSH and SH groups,
while in muscle SOD, CAT, GSH-Px, GR and SH groups). The number of
significant correlations between accumulated metals and AOS parameters
were very similar in frogs from both seasons.
All results showed that oxidative stress in the post-hibernating season was
mainly caused by the organism’s recovery from hibernation, and that the
metals did not contribute significantly to this response, indicating that natural
selection affected AOS of the frogs in that way that during arousal they were
able to produce higher levels of antioxidant defenses.",
publisher = "Association of Naturalists Students of the Jagiellonian University",
journal = "Abstract Book: Conference of young evolutionary biologist; 2017 Jun 2-4; Krakow, Poland",
title = "Heavy metals, oxidative stress and their relationship in Pelophylax ridibundus frogs during pre- and posthibernation period",
pages = "45-46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3420"
}

Prokić, M., Gavrilović, B., Krizmanić, I., Gavrić, J., Nasia, M., Borković Mitić, S., Pavlović, S., Despotović, S., Radovanović, T.,& Saičić, Z.. (2017). Heavy metals, oxidative stress and their relationship in Pelophylax ridibundus frogs during pre- and posthibernation period. in Abstract Book: Conference of young evolutionary biologist; 2017 Jun 2-4; Krakow, Poland
Association of Naturalists Students of the Jagiellonian University., 45-46.
https://hdl.handle.net/21.15107/rcub_ibiss_3420

Prokić M, Gavrilović B, Krizmanić I, Gavrić J, Nasia M, Borković Mitić S, Pavlović S, Despotović S, Radovanović T, Saičić Z. Heavy metals, oxidative stress and their relationship in Pelophylax ridibundus frogs during pre- and posthibernation period. in Abstract Book: Conference of young evolutionary biologist; 2017 Jun 2-4; Krakow, Poland. 2017;:45-46.
https://hdl.handle.net/21.15107/rcub_ibiss_3420 .

Prokić, Marko, Gavrilović, Branka, Krizmanić, Imre, Gavrić, Jelena, Nasia, Mohammed, Borković Mitić, Slavica, Pavlović, Slađan, Despotović, Svetlana, Radovanović, Tijana, Saičić, Zorica, "Heavy metals, oxidative stress and their relationship in Pelophylax ridibundus frogs during pre- and posthibernation period" in Abstract Book: Conference of young evolutionary biologist; 2017 Jun 2-4; Krakow, Poland (2017):45-46,
https://hdl.handle.net/21.15107/rcub_ibiss_3420 .

TY  - JOUR
AU  - Matić, Miloš
AU  - Paunović, Milica
AU  - Ognjanović, Branka
AU  - Štajn, Andraš
AU  - Saičić, Zorica
PY  - 2017
UR  - internal-pdf://Matić et al. - 2016 - Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative antioxidative.pdf
UR  - http://online.journals.tubitak.gov.tr/openInPressDocument.htm?fileID=799024&no=150073&fileType=Report Document
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2614
AB  - Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O2 •–, H2O2) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.
T2  - Turkish Journal of Biology
T1  - Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status
VL  - 41
DO  - 10.3906/biy-1606-73
SP  - 292
EP  - 301
ER  - 

@article{
author = "Matić, Miloš and Paunović, Milica and Ognjanović, Branka and Štajn, Andraš and Saičić, Zorica",
year = "2017",
abstract = "Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O2 •–, H2O2) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.",
journal = "Turkish Journal of Biology",
title = "Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status",
volume = "41",
doi = "10.3906/biy-1606-73",
pages = "292-301"
}

Matić, M., Paunović, M., Ognjanović, B., Štajn, A.,& Saičić, Z.. (2017). Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status. in Turkish Journal of Biology, 41, 292-301.
https://doi.org/10.3906/biy-1606-73

Matić M, Paunović M, Ognjanović B, Štajn A, Saičić Z. Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status. in Turkish Journal of Biology. 2017;41:292-301.
doi:10.3906/biy-1606-73 .

Matić, Miloš, Paunović, Milica, Ognjanović, Branka, Štajn, Andraš, Saičić, Zorica, "Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status" in Turkish Journal of Biology, 41 (2017):292-301,
https://doi.org/10.3906/biy-1606-73 . .

TY  - JOUR
AU  - Matić, Miloš
AU  - Paunović, Milica
AU  - Ognjanović, Branka
AU  - Štajn, Andraš
AU  - Saičić, Zorica
PY  - 2017
UR  - http://online.journals.tubitak.gov.tr/openInPressDocument.htm?fileID=799024&no=150073&fileType=Report Document
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2739
AB  - Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O 2 •– , H 2 O 2 ) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.
T2  - Turkish Journal of Biology
T1  - Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status
IS  - 2
VL  - 41
DO  - 10.3906/biy-1606-73
SP  - 292
EP  - 301
ER  - 

@article{
author = "Matić, Miloš and Paunović, Milica and Ognjanović, Branka and Štajn, Andraš and Saičić, Zorica",
year = "2017",
abstract = "Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O 2 •– , H 2 O 2 ) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.",
journal = "Turkish Journal of Biology",
title = "Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status",
number = "2",
volume = "41",
doi = "10.3906/biy-1606-73",
pages = "292-301"
}

Matić, M., Paunović, M., Ognjanović, B., Štajn, A.,& Saičić, Z.. (2017). Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status. in Turkish Journal of Biology, 41(2), 292-301.
https://doi.org/10.3906/biy-1606-73

Matić M, Paunović M, Ognjanović B, Štajn A, Saičić Z. Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status. in Turkish Journal of Biology. 2017;41(2):292-301.
doi:10.3906/biy-1606-73 .

Matić, Miloš, Paunović, Milica, Ognjanović, Branka, Štajn, Andraš, Saičić, Zorica, "Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status" in Turkish Journal of Biology, 41, no. 2 (2017):292-301,
https://doi.org/10.3906/biy-1606-73 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Nasia, Mohammed
AU  - Krizmanić, Imre I.
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2017
UR  - http://doi.wiley.com/10.1002/etc.3849
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3418
AB  - Pyrethroids are synthetic insecticides widely used in agriculture, public health, and veterinary medicine. Deltamethrin, a type II pyrethroid insecticide, has attracted particular attention because of its frequent use. The mechanisms of the toxicity of most pesticides (including pyrethroids) in nontarget organisms is linked to the production of free radicals, oxidative stress induction, increased lipid peroxidation, and disruption of the total antioxidant potential. The aim of the present study was to investigate the effects of acute toxicity of 3 different concentrations (8, 16, and 32 mg/kg body wt) of orally applied deltamethrin after 96 h of treatment. Some of the front-line oxidative stress parameters as well as cholinesterase (ChE) activity and cytochrome P4501A (CYP1A) expression in the liver, muscle, skin, and gastrointestinal tissue of the adult green toad Bufotes viridis were determined. Compared with the control group, the activity of catalase and glutathione reductase was increased in the liver and skin, while the concentration of sulfhydryl groups was reduced. In the liver and muscle, concentrations of thiobarbituric reactive substances were increased, as well as liver CYP1A expression. In the muscle and skin, glutathione-S-transferase activity was higher in treated toads. The oxidative stress parameters examined were affected by different deltamethrin concentrations. We conclude that the assessed parameters represent good biomarkers of pesticide-induced oxidative stress. Environ Toxicol Chem 2017;36:2814-2822. © 2017 SETAC.
T2  - Environmental Toxicology and Chemistry
T1  - Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).
IS  - 10
VL  - 36
DO  - 10.1002/etc.3849
SP  - 2814
EP  - 2822
ER  - 

@article{
author = "Radovanović, Tijana and Nasia, Mohammed and Krizmanić, Imre I. and Prokić, Marko and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2017",
abstract = "Pyrethroids are synthetic insecticides widely used in agriculture, public health, and veterinary medicine. Deltamethrin, a type II pyrethroid insecticide, has attracted particular attention because of its frequent use. The mechanisms of the toxicity of most pesticides (including pyrethroids) in nontarget organisms is linked to the production of free radicals, oxidative stress induction, increased lipid peroxidation, and disruption of the total antioxidant potential. The aim of the present study was to investigate the effects of acute toxicity of 3 different concentrations (8, 16, and 32 mg/kg body wt) of orally applied deltamethrin after 96 h of treatment. Some of the front-line oxidative stress parameters as well as cholinesterase (ChE) activity and cytochrome P4501A (CYP1A) expression in the liver, muscle, skin, and gastrointestinal tissue of the adult green toad Bufotes viridis were determined. Compared with the control group, the activity of catalase and glutathione reductase was increased in the liver and skin, while the concentration of sulfhydryl groups was reduced. In the liver and muscle, concentrations of thiobarbituric reactive substances were increased, as well as liver CYP1A expression. In the muscle and skin, glutathione-S-transferase activity was higher in treated toads. The oxidative stress parameters examined were affected by different deltamethrin concentrations. We conclude that the assessed parameters represent good biomarkers of pesticide-induced oxidative stress. Environ Toxicol Chem 2017;36:2814-2822. © 2017 SETAC.",
journal = "Environmental Toxicology and Chemistry",
title = "Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).",
number = "10",
volume = "36",
doi = "10.1002/etc.3849",
pages = "2814-2822"
}

Radovanović, T., Nasia, M., Krizmanić, I. I., Prokić, M., Gavrić, J., Despotović, S., Gavrilović, B., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2017). Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).. in Environmental Toxicology and Chemistry, 36(10), 2814-2822.
https://doi.org/10.1002/etc.3849

Radovanović T, Nasia M, Krizmanić II, Prokić M, Gavrić J, Despotović S, Gavrilović B, Borković Mitić S, Pavlović S, Saičić Z. Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).. in Environmental Toxicology and Chemistry. 2017;36(10):2814-2822.
doi:10.1002/etc.3849 .

Radovanović, Tijana, Nasia, Mohammed, Krizmanić, Imre I., Prokić, Marko, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.)." in Environmental Toxicology and Chemistry, 36, no. 10 (2017):2814-2822,
https://doi.org/10.1002/etc.3849 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Borković Mitić, Slavica
AU  - Krizmanić, Imre
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2017
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5198921
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3417
AB  - Comparative activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), the phase II biotransformation enzyme glutathione-S-transferase (GST), the concentrations of total glutathione (GSH), sulfhydryl groups (-SH) and the activity of the neurotoxicity biomarker acetylcholinesterase (AChE) were investigated in the livers of species belonging to the Pelophylax esculentus "complex" (parental species Pelophylax ridibundus, Pelophylax lessonae, and their hybrid Pelophylax kl. esculentus) from the wetland, Obedska bara in Serbia. The condition factor (CF) and hepato somatic index (HSI) were also calculated. All three species were caught at same locality and were exposed to the same environmental conditions. Liver SOD activity was lower in P. ridibundus than in P. kl. esculentus and P. lessonae; higher activities of CAT, GR and GST were observed in P. kl. esculentus frogs as compared to their parental species. The activity of GSH-Px was significantly lower in P. kl. esculentus. The activity of AChE was increased in P. lessonae as compared to P. kl. esculentus and P. ridibundus. Similar concentrations of GSH and -SH groups were observed in all investigated species. P. kl. esculentus had a higher CF, while the HSI was lower when compared to the parental species. Our findings suggest that the parental species (P. ridibundus and P. lessonae) possess more similar antioxidative responses to environmental conditions than the hybrid species P. kl. esculentus. The obtained results improve our understanding of the biology and physiology of these three closely related species.
T2  - Saudi Journal of Biological Sciences
T1  - Comparative study of oxidative stress parameters and acetylcholinesterase activity in the liver of Pelophylax esculentus complex frogs
IS  - 1
VL  - 24
DO  - 10.1016/j.sjbs.2015.09.003
SP  - 51
EP  - 58
ER  - 

@article{
author = "Prokić, Marko and Borković Mitić, Slavica and Krizmanić, Imre and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Pavlović, Slađan and Saičić, Zorica",
year = "2017",
abstract = "Comparative activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), the phase II biotransformation enzyme glutathione-S-transferase (GST), the concentrations of total glutathione (GSH), sulfhydryl groups (-SH) and the activity of the neurotoxicity biomarker acetylcholinesterase (AChE) were investigated in the livers of species belonging to the Pelophylax esculentus "complex" (parental species Pelophylax ridibundus, Pelophylax lessonae, and their hybrid Pelophylax kl. esculentus) from the wetland, Obedska bara in Serbia. The condition factor (CF) and hepato somatic index (HSI) were also calculated. All three species were caught at same locality and were exposed to the same environmental conditions. Liver SOD activity was lower in P. ridibundus than in P. kl. esculentus and P. lessonae; higher activities of CAT, GR and GST were observed in P. kl. esculentus frogs as compared to their parental species. The activity of GSH-Px was significantly lower in P. kl. esculentus. The activity of AChE was increased in P. lessonae as compared to P. kl. esculentus and P. ridibundus. Similar concentrations of GSH and -SH groups were observed in all investigated species. P. kl. esculentus had a higher CF, while the HSI was lower when compared to the parental species. Our findings suggest that the parental species (P. ridibundus and P. lessonae) possess more similar antioxidative responses to environmental conditions than the hybrid species P. kl. esculentus. The obtained results improve our understanding of the biology and physiology of these three closely related species.",
journal = "Saudi Journal of Biological Sciences",
title = "Comparative study of oxidative stress parameters and acetylcholinesterase activity in the liver of Pelophylax esculentus complex frogs",
number = "1",
volume = "24",
doi = "10.1016/j.sjbs.2015.09.003",
pages = "51-58"
}

Prokić, M., Borković Mitić, S., Krizmanić, I., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Pavlović, S.,& Saičić, Z.. (2017). Comparative study of oxidative stress parameters and acetylcholinesterase activity in the liver of Pelophylax esculentus complex frogs. in Saudi Journal of Biological Sciences, 24(1), 51-58.
https://doi.org/10.1016/j.sjbs.2015.09.003

Prokić M, Borković Mitić S, Krizmanić I, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Pavlović S, Saičić Z. Comparative study of oxidative stress parameters and acetylcholinesterase activity in the liver of Pelophylax esculentus complex frogs. in Saudi Journal of Biological Sciences. 2017;24(1):51-58.
doi:10.1016/j.sjbs.2015.09.003 .

Prokić, Marko, Borković Mitić, Slavica, Krizmanić, Imre, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Pavlović, Slađan, Saičić, Zorica, "Comparative study of oxidative stress parameters and acetylcholinesterase activity in the liver of Pelophylax esculentus complex frogs" in Saudi Journal of Biological Sciences, 24, no. 1 (2017):51-58,
https://doi.org/10.1016/j.sjbs.2015.09.003 . .

TY  - JOUR
AU  - Milošević, Marija D.
AU  - Paunović, Milica G.
AU  - Matić, Miloš M.
AU  - Ognjanović, Branka I.
AU  - Saičić, Zorica
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1382668917302740
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2863
AB  - Fenitrothion is widely used organophosphate pesticide in agriculture and health programs, but besides, it causes several toxic effects. The present study was designed to evaluate the possible protective effects of selenium (0.5 mg/kg b.w.) and vitamin C (100 mg/kg b.w) on altered haematological, biochemical and oxidative stress parameters in the blood of rats orally treated with fenitrothion (20 mg/kg b.w) for 30 days. Fenitrothion caused changes in body weight, food and water intake, and some haematological and biochemical parameters. Fenitrothion altered the glutathione redox status (GSH and GSSG) and decreased activity of antioxidant enzymes (GSH-Px, GST, SOD and CAT), leading to a lipid peroxidation. Selenium and vitamin C, by improving the activity of antioxidants, reduced oxidative stress and a lipid peroxidation, maintaining the values of examined parameters to optimal levels. Therefore, selenium and vitamin C could be useful in providing protection of exposed non-target organisms including people from fenitrothion.
T2  - Environmental Toxicology and Pharmacology
T1  - The ameliorating effects of selenium and vitamin C against fenitrothion-induced blood toxicity in Wistar rats
VL  - 56
DO  - 10.1016/j.etap.2017.09.016
SP  - 204
EP  - 209
ER  - 

@article{
author = "Milošević, Marija D. and Paunović, Milica G. and Matić, Miloš M. and Ognjanović, Branka I. and Saičić, Zorica",
year = "2017",
abstract = "Fenitrothion is widely used organophosphate pesticide in agriculture and health programs, but besides, it causes several toxic effects. The present study was designed to evaluate the possible protective effects of selenium (0.5 mg/kg b.w.) and vitamin C (100 mg/kg b.w) on altered haematological, biochemical and oxidative stress parameters in the blood of rats orally treated with fenitrothion (20 mg/kg b.w) for 30 days. Fenitrothion caused changes in body weight, food and water intake, and some haematological and biochemical parameters. Fenitrothion altered the glutathione redox status (GSH and GSSG) and decreased activity of antioxidant enzymes (GSH-Px, GST, SOD and CAT), leading to a lipid peroxidation. Selenium and vitamin C, by improving the activity of antioxidants, reduced oxidative stress and a lipid peroxidation, maintaining the values of examined parameters to optimal levels. Therefore, selenium and vitamin C could be useful in providing protection of exposed non-target organisms including people from fenitrothion.",
journal = "Environmental Toxicology and Pharmacology",
title = "The ameliorating effects of selenium and vitamin C against fenitrothion-induced blood toxicity in Wistar rats",
volume = "56",
doi = "10.1016/j.etap.2017.09.016",
pages = "204-209"
}

Milošević, M. D., Paunović, M. G., Matić, M. M., Ognjanović, B. I.,& Saičić, Z.. (2017). The ameliorating effects of selenium and vitamin C against fenitrothion-induced blood toxicity in Wistar rats. in Environmental Toxicology and Pharmacology, 56, 204-209.
https://doi.org/10.1016/j.etap.2017.09.016

Milošević MD, Paunović MG, Matić MM, Ognjanović BI, Saičić Z. The ameliorating effects of selenium and vitamin C against fenitrothion-induced blood toxicity in Wistar rats. in Environmental Toxicology and Pharmacology. 2017;56:204-209.
doi:10.1016/j.etap.2017.09.016 .

Milošević, Marija D., Paunović, Milica G., Matić, Miloš M., Ognjanović, Branka I., Saičić, Zorica, "The ameliorating effects of selenium and vitamin C against fenitrothion-induced blood toxicity in Wistar rats" in Environmental Toxicology and Pharmacology, 56 (2017):204-209,
https://doi.org/10.1016/j.etap.2017.09.016 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Borković Mitić, Slavica
AU  - Krizmanić, Imre I.
AU  - Mutić, Jelena J.
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1532045617301370
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2827
AB  - In spring, frogs from temperate regions are faced with arousal-induced oxidative stress and exposure to various xenobiotics from the environment. The question is whether pollutants can significantly modify the antioxidative defense system (AOS) response of hibernators during recovery from hibernation. If this assumption is true, we would then expect different patterns of seasonal variations in the AOS between individuals exposed to different levels of pollution. To examine this assumption, we determined the relationship between seasonal variations of accumulated metals and AOS parameters in the skin and muscle of two frog species from the Pelophylax esculentus complex (P. ridibundus and P. esculentus) inhabiting two localities (the Danube-Tisza-Danube canal and the Ponjavica River) with different levels of pollution during pre- and post-hibernation periods, respectively autumn and spring. Our results showed that even though there were differences in the concentrations of accumulated metals and AOS parameters between localities and species, the frogs displayed almost the same patterns of AOS variations during seasons, with a higher AOS response observed in spring. The parameters SH groups, GSH, GR and SOD had been contributed most rather than others. Our findings indicate that oxidative stress during the post-hibernation period was mainly caused by the organisms’ recovery from hibernation, as the result of natural selection acting on the AOS, and that the accumulated metals did not significantly modify the AOS response. The present study provides new insight into the biological and physiological cellular responses of frogs to arousal stress.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals
VL  - 202
DO  - 10.1016/j.cbpc.2017.07.006
SP  - 19
EP  - 25
ER  - 

@article{
author = "Prokić, Marko and Borković Mitić, Slavica and Krizmanić, Imre I. and Mutić, Jelena J. and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Pavlović, Slađan and Saičić, Zorica",
year = "2017",
abstract = "In spring, frogs from temperate regions are faced with arousal-induced oxidative stress and exposure to various xenobiotics from the environment. The question is whether pollutants can significantly modify the antioxidative defense system (AOS) response of hibernators during recovery from hibernation. If this assumption is true, we would then expect different patterns of seasonal variations in the AOS between individuals exposed to different levels of pollution. To examine this assumption, we determined the relationship between seasonal variations of accumulated metals and AOS parameters in the skin and muscle of two frog species from the Pelophylax esculentus complex (P. ridibundus and P. esculentus) inhabiting two localities (the Danube-Tisza-Danube canal and the Ponjavica River) with different levels of pollution during pre- and post-hibernation periods, respectively autumn and spring. Our results showed that even though there were differences in the concentrations of accumulated metals and AOS parameters between localities and species, the frogs displayed almost the same patterns of AOS variations during seasons, with a higher AOS response observed in spring. The parameters SH groups, GSH, GR and SOD had been contributed most rather than others. Our findings indicate that oxidative stress during the post-hibernation period was mainly caused by the organisms’ recovery from hibernation, as the result of natural selection acting on the AOS, and that the accumulated metals did not significantly modify the AOS response. The present study provides new insight into the biological and physiological cellular responses of frogs to arousal stress.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals",
volume = "202",
doi = "10.1016/j.cbpc.2017.07.006",
pages = "19-25"
}

Prokić, M., Borković Mitić, S., Krizmanić, I. I., Mutić, J. J., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Pavlović, S.,& Saičić, Z.. (2017). Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 202, 19-25.
https://doi.org/10.1016/j.cbpc.2017.07.006

Prokić M, Borković Mitić S, Krizmanić II, Mutić JJ, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Pavlović S, Saičić Z. Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2017;202:19-25.
doi:10.1016/j.cbpc.2017.07.006 .

Prokić, Marko, Borković Mitić, Slavica, Krizmanić, Imre I., Mutić, Jelena J., Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Pavlović, Slađan, Saičić, Zorica, "Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 202 (2017):19-25,
https://doi.org/10.1016/j.cbpc.2017.07.006 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Anđelković, Marko
AU  - Tomović, Ljiljana
AU  - Prokić, Marko
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0147651316305449
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2498
AB  - We investigated in the liver of dice snakes during pre- and post-hibernation changes in the following antioxidant parameters: total, manganese and copper zinc containing superoxide dismutases (Tot SOD, MnSOD, CuZn SOD, respectively), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the concentrations of total glutathione (GSH) and sulfhydryl groups (–SH). In addition, we examined the expression of phase I biotransformation enzyme cytochrome P4501A (CYP1A) and the activity of phase II biotransformation enzyme glutathioneS-transferase (GST), the level of lipid peroxidation (by measuring the thiobarbituric acid-reactive substances (TBARS)), cholinesterase activity (ChE) and metallothionein expression (MT). We also measured the concentrations of heavy metals, including Al, Cd, As, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb and Zn in the water and snake liver during both periods. During the post-hibernation period, the activities of Tot SOD, CuZn SOD and GST and the concentration of GSH were significantly decreased, while GSH-Px and GR activities, the concentrations of –SH groups and TBARS were significantly increased. The activities of Mn SOD, CAT and ChE, and the relative amounts of CYP1A and MT did not significantly change during the investigated periods. The observed differences in the examined parameters probably represent adaptive physiological responses to sudden changes in tissue oxygenation during arousal from hibernation. Our findings also indicate that the accumulated metals modulated the responses of the examined parameters during the investigated periods.
T2  - Ecotoxicology and Environmental Safety
T1  - Oxidative stress biomarkers, cholinesterase activity and biotransformation enzymes in the liver of dice snake (Natrix tessellata Laurenti) during pre-hibernation and post-hibernation: A possible correlation with heavy metals in the environment
VL  - 138
DO  - 10.1016/j.ecoenv.2016.12.036
SP  - 154
EP  - 162
ER  - 

@article{
author = "Gavrić, Jelena and Anđelković, Marko and Tomović, Ljiljana and Prokić, Marko and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2017",
abstract = "We investigated in the liver of dice snakes during pre- and post-hibernation changes in the following antioxidant parameters: total, manganese and copper zinc containing superoxide dismutases (Tot SOD, MnSOD, CuZn SOD, respectively), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the concentrations of total glutathione (GSH) and sulfhydryl groups (–SH). In addition, we examined the expression of phase I biotransformation enzyme cytochrome P4501A (CYP1A) and the activity of phase II biotransformation enzyme glutathioneS-transferase (GST), the level of lipid peroxidation (by measuring the thiobarbituric acid-reactive substances (TBARS)), cholinesterase activity (ChE) and metallothionein expression (MT). We also measured the concentrations of heavy metals, including Al, Cd, As, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb and Zn in the water and snake liver during both periods. During the post-hibernation period, the activities of Tot SOD, CuZn SOD and GST and the concentration of GSH were significantly decreased, while GSH-Px and GR activities, the concentrations of –SH groups and TBARS were significantly increased. The activities of Mn SOD, CAT and ChE, and the relative amounts of CYP1A and MT did not significantly change during the investigated periods. The observed differences in the examined parameters probably represent adaptive physiological responses to sudden changes in tissue oxygenation during arousal from hibernation. Our findings also indicate that the accumulated metals modulated the responses of the examined parameters during the investigated periods.",
journal = "Ecotoxicology and Environmental Safety",
title = "Oxidative stress biomarkers, cholinesterase activity and biotransformation enzymes in the liver of dice snake (Natrix tessellata Laurenti) during pre-hibernation and post-hibernation: A possible correlation with heavy metals in the environment",
volume = "138",
doi = "10.1016/j.ecoenv.2016.12.036",
pages = "154-162"
}

Gavrić, J., Anđelković, M., Tomović, L., Prokić, M., Despotović, S., Gavrilović, B., Radovanović, T., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2017). Oxidative stress biomarkers, cholinesterase activity and biotransformation enzymes in the liver of dice snake (Natrix tessellata Laurenti) during pre-hibernation and post-hibernation: A possible correlation with heavy metals in the environment. in Ecotoxicology and Environmental Safety, 138, 154-162.
https://doi.org/10.1016/j.ecoenv.2016.12.036

Gavrić J, Anđelković M, Tomović L, Prokić M, Despotović S, Gavrilović B, Radovanović T, Borković Mitić S, Pavlović S, Saičić Z. Oxidative stress biomarkers, cholinesterase activity and biotransformation enzymes in the liver of dice snake (Natrix tessellata Laurenti) during pre-hibernation and post-hibernation: A possible correlation with heavy metals in the environment. in Ecotoxicology and Environmental Safety. 2017;138:154-162.
doi:10.1016/j.ecoenv.2016.12.036 .

Gavrić, Jelena, Anđelković, Marko, Tomović, Ljiljana, Prokić, Marko, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Oxidative stress biomarkers, cholinesterase activity and biotransformation enzymes in the liver of dice snake (Natrix tessellata Laurenti) during pre-hibernation and post-hibernation: A possible correlation with heavy metals in the environment" in Ecotoxicology and Environmental Safety, 138 (2017):154-162,
https://doi.org/10.1016/j.ecoenv.2016.12.036 . .

TY  - JOUR
AU  - Paunović, Milica G
AU  - Matić, Miloš M
AU  - Ognjanović, Branka I
AU  - Saičić, Zorica
PY  - 2017
UR  - http://journals.sagepub.com/doi/10.1177/0748233717725480
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28950793
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2889
AB  - Cadmium (Cd) is a major environmental pollutant, which exerts adverse effects mainly by inducing oxidative stress. Coenzyme Q10 (CoQ10) and vitamin E (VE), naturally occurring antioxidants, improve health condition by inactivating free radicals and enhancing antioxidative defence. The aim of our study was to investigate the protective role of CoQ10 and/or VE pretreatment against Cd-induced haematotoxicity. Wistar albino rats were intramuscularly injected with CoQ10 (20 mg/kg b.w.) and/or VE (20 IU/kg b.w.) or with saline (control group). After 24 h, Cd was injected intraperitoneally (0.4 mg/kg b.w.) and 1 day after, animals were sacrificed. Acute Cd intoxication caused significant changes in haematological and biochemical parameters and altered the glutathione cycle, leading to the formation of lipid peroxidation, while the concentrations and activities of antioxidants (vitamins C and E, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase) were decreased. CoQ10 and/or VE significantly maintained these values to near-normal levels, afforded additional protection by reducing lipid peroxidation and improved the levels of antioxidants in the blood. Plasma CoQ10 and VE levels negatively correlated with oxidative damage parameters while positively correlated with antioxidative defence parameters. Regarding their effects, CoQ10 and VE were in synergistic interaction. The present study suggested that CoQ10 and VE combination may be beneficial in protecting from Cd-induced haematotoxicity and may be used as a preventive against acute Cd intoxication of exposed people.
T2  - Toxicology and industrial health
T1  - Antioxidative and haematoprotective activity of coenzyme Q10 and vitamin E against cadmium-induced oxidative stress in Wistar rats.
IS  - 10
VL  - 33
DO  - 10.1177/0748233717725480
SP  - 746
EP  - 756
ER  - 

@article{
author = "Paunović, Milica G and Matić, Miloš M and Ognjanović, Branka I and Saičić, Zorica",
year = "2017",
abstract = "Cadmium (Cd) is a major environmental pollutant, which exerts adverse effects mainly by inducing oxidative stress. Coenzyme Q10 (CoQ10) and vitamin E (VE), naturally occurring antioxidants, improve health condition by inactivating free radicals and enhancing antioxidative defence. The aim of our study was to investigate the protective role of CoQ10 and/or VE pretreatment against Cd-induced haematotoxicity. Wistar albino rats were intramuscularly injected with CoQ10 (20 mg/kg b.w.) and/or VE (20 IU/kg b.w.) or with saline (control group). After 24 h, Cd was injected intraperitoneally (0.4 mg/kg b.w.) and 1 day after, animals were sacrificed. Acute Cd intoxication caused significant changes in haematological and biochemical parameters and altered the glutathione cycle, leading to the formation of lipid peroxidation, while the concentrations and activities of antioxidants (vitamins C and E, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase) were decreased. CoQ10 and/or VE significantly maintained these values to near-normal levels, afforded additional protection by reducing lipid peroxidation and improved the levels of antioxidants in the blood. Plasma CoQ10 and VE levels negatively correlated with oxidative damage parameters while positively correlated with antioxidative defence parameters. Regarding their effects, CoQ10 and VE were in synergistic interaction. The present study suggested that CoQ10 and VE combination may be beneficial in protecting from Cd-induced haematotoxicity and may be used as a preventive against acute Cd intoxication of exposed people.",
journal = "Toxicology and industrial health",
title = "Antioxidative and haematoprotective activity of coenzyme Q10 and vitamin E against cadmium-induced oxidative stress in Wistar rats.",
number = "10",
volume = "33",
doi = "10.1177/0748233717725480",
pages = "746-756"
}

Paunović, M. G., Matić, M. M., Ognjanović, B. I.,& Saičić, Z.. (2017). Antioxidative and haematoprotective activity of coenzyme Q10 and vitamin E against cadmium-induced oxidative stress in Wistar rats.. in Toxicology and industrial health, 33(10), 746-756.
https://doi.org/10.1177/0748233717725480

Paunović MG, Matić MM, Ognjanović BI, Saičić Z. Antioxidative and haematoprotective activity of coenzyme Q10 and vitamin E against cadmium-induced oxidative stress in Wistar rats.. in Toxicology and industrial health. 2017;33(10):746-756.
doi:10.1177/0748233717725480 .

Paunović, Milica G, Matić, Miloš M, Ognjanović, Branka I, Saičić, Zorica, "Antioxidative and haematoprotective activity of coenzyme Q10 and vitamin E against cadmium-induced oxidative stress in Wistar rats." in Toxicology and industrial health, 33, no. 10 (2017):746-756,
https://doi.org/10.1177/0748233717725480 . .

TY  - CONF
AU  - Nasia, Mohammed
AU  - Gavrilović, Branka
AU  - Krizmanić, Imre
AU  - Gavrić, Jelena
AU  - Prokić, Marko
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Despotović, Svetlana
AU  - Radovanović, Tijana
AU  - Saičić, Zorica
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3421
AB  - The highest goal of every species is to survival, reproduce and continue
existence. To fulfill these frogs needs to survive larval and subadult stages
until gain sexual maturity. During this they are exposed to different stressors.
Resistances to external or internal stressors are fitness-related traits that are
central to evolutionary research (Monaghan et al., 2009). One example is
resistance to oxidative stress (OS), which has been suggested to affect lifehistory
trade-offs and fitness (Pamplona and Costantini, 2011). To protect
against oxidative stress, organisms have developed the antioxidative defense
system (AOS), which is comprised of enzymatic (including the activities of
superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx),
glutathione reductase (GR), phase II biotransformation enzyme
glutathione-S-transferase (GST)) and nonenzymatic (the total glutathione
(GSH) contents and sulfhydryl (SH) group concentrations) components. It was
suggested that in younger individuals OS was mainly caused by increased
growing rate and metabolic activity, while in adults it was not only arised
from endogenous metabolism, but also from oxidative challenges induced by
environmental conditions (Metcalfe and Alonso-Alvarez, 2010). In this study we tried to examine AOS of liver of subadult and adult
Bufotes viridis frogs caught from natural population and kept in laboratory
conditions for 14 days, in order to determine possible differences in AOS that
could be related to the frog’s body size and life stage (adults and subadults).
The results showed higher activities of SOD, GR and GST in subadults in
comparison to adults, on the other hand adults had higher activity of GSH-Px
and concentrations of GSH and SH- groups. CAT was the only parameter that
did not differ significantly between groups and did not correlate with frog’s
snout-vent length (SVL). Significant negative correlations were noticed
between frogs SVL and activity of SOD (r=-0.69) and GST (r=-0.45), while
positive correlations of SVL were with GSH-Px (r=0.63), GSH (r=0.67) and SH
groups (r=0.52).
Based on the results we conclude that in subadults enzymatic
components of AOS (SOD, GR and GST) played most important role in
response to OS, while in adults it were nonenzimatic components (GSH and
SH) that were already marked as biomarkers of some xenobiotics that tend to
accumulate.
PB  - Association of Naturalists Students of the Jagiellonian University
C3  - Conference of young evolutionary biologist: Abstract Book. Krakow, Poland; 02-04, June 2017
T1  - Antioxidative defense system in liver of adult and subadult Bufotes viridis frogs
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3421
ER  - 

@conference{
author = "Nasia, Mohammed and Gavrilović, Branka and Krizmanić, Imre and Gavrić, Jelena and Prokić, Marko and Borković Mitić, Slavica and Pavlović, Slađan and Despotović, Svetlana and Radovanović, Tijana and Saičić, Zorica",
year = "2017",
abstract = "The highest goal of every species is to survival, reproduce and continue
existence. To fulfill these frogs needs to survive larval and subadult stages
until gain sexual maturity. During this they are exposed to different stressors.
Resistances to external or internal stressors are fitness-related traits that are
central to evolutionary research (Monaghan et al., 2009). One example is
resistance to oxidative stress (OS), which has been suggested to affect lifehistory
trade-offs and fitness (Pamplona and Costantini, 2011). To protect
against oxidative stress, organisms have developed the antioxidative defense
system (AOS), which is comprised of enzymatic (including the activities of
superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx),
glutathione reductase (GR), phase II biotransformation enzyme
glutathione-S-transferase (GST)) and nonenzymatic (the total glutathione
(GSH) contents and sulfhydryl (SH) group concentrations) components. It was
suggested that in younger individuals OS was mainly caused by increased
growing rate and metabolic activity, while in adults it was not only arised
from endogenous metabolism, but also from oxidative challenges induced by
environmental conditions (Metcalfe and Alonso-Alvarez, 2010). In this study we tried to examine AOS of liver of subadult and adult
Bufotes viridis frogs caught from natural population and kept in laboratory
conditions for 14 days, in order to determine possible differences in AOS that
could be related to the frog’s body size and life stage (adults and subadults).
The results showed higher activities of SOD, GR and GST in subadults in
comparison to adults, on the other hand adults had higher activity of GSH-Px
and concentrations of GSH and SH- groups. CAT was the only parameter that
did not differ significantly between groups and did not correlate with frog’s
snout-vent length (SVL). Significant negative correlations were noticed
between frogs SVL and activity of SOD (r=-0.69) and GST (r=-0.45), while
positive correlations of SVL were with GSH-Px (r=0.63), GSH (r=0.67) and SH
groups (r=0.52).
Based on the results we conclude that in subadults enzymatic
components of AOS (SOD, GR and GST) played most important role in
response to OS, while in adults it were nonenzimatic components (GSH and
SH) that were already marked as biomarkers of some xenobiotics that tend to
accumulate.",
publisher = "Association of Naturalists Students of the Jagiellonian University",
journal = "Conference of young evolutionary biologist: Abstract Book. Krakow, Poland; 02-04, June 2017",
title = "Antioxidative defense system in liver of adult and subadult Bufotes viridis frogs",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3421"
}

Nasia, M., Gavrilović, B., Krizmanić, I., Gavrić, J., Prokić, M., Borković Mitić, S., Pavlović, S., Despotović, S., Radovanović, T.,& Saičić, Z.. (2017). Antioxidative defense system in liver of adult and subadult Bufotes viridis frogs. in Conference of young evolutionary biologist: Abstract Book. Krakow, Poland; 02-04, June 2017
Association of Naturalists Students of the Jagiellonian University., 47-48.
https://hdl.handle.net/21.15107/rcub_ibiss_3421

Nasia M, Gavrilović B, Krizmanić I, Gavrić J, Prokić M, Borković Mitić S, Pavlović S, Despotović S, Radovanović T, Saičić Z. Antioxidative defense system in liver of adult and subadult Bufotes viridis frogs. in Conference of young evolutionary biologist: Abstract Book. Krakow, Poland; 02-04, June 2017. 2017;:47-48.
https://hdl.handle.net/21.15107/rcub_ibiss_3421 .

Nasia, Mohammed, Gavrilović, Branka, Krizmanić, Imre, Gavrić, Jelena, Prokić, Marko, Borković Mitić, Slavica, Pavlović, Slađan, Despotović, Svetlana, Radovanović, Tijana, Saičić, Zorica, "Antioxidative defense system in liver of adult and subadult Bufotes viridis frogs" in Conference of young evolutionary biologist: Abstract Book. Krakow, Poland; 02-04, June 2017 (2017):47-48,
https://hdl.handle.net/21.15107/rcub_ibiss_3421 .

TY  - JOUR
AU  - Prokić, Marko
AU  - Borković-Mitić, Slavica
AU  - Krizmanić, Imre
AU  - Mutić, Jelena
AU  - Vukojević, Vesna
AU  - Nasia, Mohamed
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5931
AB  - Heavy metal pollution of the aquatic environment is of great concern worldwide. Heavy metals are
capable of inducing oxidative stress by increasing the formation of reactive oxygen species (ROS), and
directly affecting the antioxidant defense system (AOS) in living organisms. The frog Pelophylax kl. esculentus is a semiaquatic species with semipermeable skin and a complex lifecycle, and represents a
potentially useful bioindicator organism. The aim of this study was to investigate the accumulation of
several heavy metals (Cd, Co, Cr, Cu, Fe, Hg, Ni, Pb and Zn), and their effects on selected parameters of the
AOS, including the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR), phase II biotransformation enzyme glutathione-S-transferase (GST), the
total glutathione (GSH) contents and sulfhydryl (SH) group concentrations, as well as cholinesterases
(ChEs) activities in the liver, skin and muscle of P. kl. esculentus. Frog samples were collected at two sites
(the Danube–Tisza–Danube canal (DTDC) and the river Ponjavica) in Serbia, which are characterized by
different levels of metal pollution. Differences between the metal contents in different tissues showed
that the skin of frogs from the DTDC accumulated statistically higher concentrations of Cd, Cu, Pb and Zn,
while only the Fe concentration was lower. No significant differences between metal concentrations in
muscle tissues of frogs from the DTDC and Ponjavica were observed. Examination of the parameters of
the AOS revealed that frogs from the DTDC had higher concentrations of GSH in the liver and of SH
groups in the skin and muscle, whereas the activities of the antioxidative enzymes SOD, GHS-Px and GR
in the liver and of GR in the skin were lower than in frogs from the Ponjavica. The relationship between
metal concentrations and AOS parameters showed the highest number of correlations with GSH, GR and
CAT, and with Ni, Zn, Hg, Cr and Cd. Based on the results in this study, we concluded that increased
concentrations of heavy metals in frog tissues can alter the AOS, which leads to higher concentrations of
GSH and SH groups and lower activities of antioxidative enzymes. The response of the AOS to metal
pollutants allowed us to make a distinction between different frog tissues, and to conclude that the liver
and skin are more suitable for assessing metal-induced oxidative stress in frogs than muscle.
PB  - San Diego: Academic Press Inc.
T2  - Ecotoxicology and Environmental Safety
T1  - Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution
VL  - 128
DO  - 10.1016/j.ecoenv.2016.02.005
SP  - 21
EP  - 29
ER  - 

@article{
author = "Prokić, Marko and Borković-Mitić, Slavica and Krizmanić, Imre and Mutić, Jelena and Vukojević, Vesna and Nasia, Mohamed and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Pavlović, Slađan and Saičić, Zorica",
year = "2016",
abstract = "Heavy metal pollution of the aquatic environment is of great concern worldwide. Heavy metals are
capable of inducing oxidative stress by increasing the formation of reactive oxygen species (ROS), and
directly affecting the antioxidant defense system (AOS) in living organisms. The frog Pelophylax kl. esculentus is a semiaquatic species with semipermeable skin and a complex lifecycle, and represents a
potentially useful bioindicator organism. The aim of this study was to investigate the accumulation of
several heavy metals (Cd, Co, Cr, Cu, Fe, Hg, Ni, Pb and Zn), and their effects on selected parameters of the
AOS, including the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR), phase II biotransformation enzyme glutathione-S-transferase (GST), the
total glutathione (GSH) contents and sulfhydryl (SH) group concentrations, as well as cholinesterases
(ChEs) activities in the liver, skin and muscle of P. kl. esculentus. Frog samples were collected at two sites
(the Danube–Tisza–Danube canal (DTDC) and the river Ponjavica) in Serbia, which are characterized by
different levels of metal pollution. Differences between the metal contents in different tissues showed
that the skin of frogs from the DTDC accumulated statistically higher concentrations of Cd, Cu, Pb and Zn,
while only the Fe concentration was lower. No significant differences between metal concentrations in
muscle tissues of frogs from the DTDC and Ponjavica were observed. Examination of the parameters of
the AOS revealed that frogs from the DTDC had higher concentrations of GSH in the liver and of SH
groups in the skin and muscle, whereas the activities of the antioxidative enzymes SOD, GHS-Px and GR
in the liver and of GR in the skin were lower than in frogs from the Ponjavica. The relationship between
metal concentrations and AOS parameters showed the highest number of correlations with GSH, GR and
CAT, and with Ni, Zn, Hg, Cr and Cd. Based on the results in this study, we concluded that increased
concentrations of heavy metals in frog tissues can alter the AOS, which leads to higher concentrations of
GSH and SH groups and lower activities of antioxidative enzymes. The response of the AOS to metal
pollutants allowed us to make a distinction between different frog tissues, and to conclude that the liver
and skin are more suitable for assessing metal-induced oxidative stress in frogs than muscle.",
publisher = "San Diego: Academic Press Inc.",
journal = "Ecotoxicology and Environmental Safety",
title = "Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution",
volume = "128",
doi = "10.1016/j.ecoenv.2016.02.005",
pages = "21-29"
}

Prokić, M., Borković-Mitić, S., Krizmanić, I., Mutić, J., Vukojević, V., Nasia, M., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Pavlović, S.,& Saičić, Z.. (2016). Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution. in Ecotoxicology and Environmental Safety
San Diego: Academic Press Inc.., 128, 21-29.
https://doi.org/10.1016/j.ecoenv.2016.02.005

Prokić M, Borković-Mitić S, Krizmanić I, Mutić J, Vukojević V, Nasia M, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Pavlović S, Saičić Z. Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution. in Ecotoxicology and Environmental Safety. 2016;128:21-29.
doi:10.1016/j.ecoenv.2016.02.005 .

Prokić, Marko, Borković-Mitić, Slavica, Krizmanić, Imre, Mutić, Jelena, Vukojević, Vesna, Nasia, Mohamed, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Pavlović, Slađan, Saičić, Zorica, "Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution" in Ecotoxicology and Environmental Safety, 128 (2016):21-29,
https://doi.org/10.1016/j.ecoenv.2016.02.005 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Borković-Mitić, Slavica
AU  - Krizmanić, Imre
AU  - Mutić, Jelena
AU  - Trifković, Jelena
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5930
AB  - Metals are involved in the formation of reactive
oxygen species and can induce oxidative stress. The aim of
this study was to assess the effects of several metals on
oxidative stress in the skin and muscle of the Pelophylax
esculentus “complex” frogs (parental species Pelophylax
ridibundus, Pelophylax lessonae, and their hybrid Pelophylax esculentus) that inhabit the wetland Obedska Bara in
Serbia, and the potential use of these species as bioindicator
organisms in biomonitoring studies. The biomarkers of
oxidative stress (SOD, CAT, GSH-Px, GR, GST activities
and GSH, SH concentrations) and cholinesterase activity
were investigated. The concentrations of nine metals (Fe,
Cu, Zn, As, Cd, Cr, Hg, Ni, and Pb) were measured in the
water and tissues. Correlations were established between
metals and biomarkers in the tissues. The results of metal
accumulation distinguished the skin of P. lessonae and
muscle of P. ridibundus from other P. esculentus complex
species. The oxidative stress biomarkers observed in P.
ridibundus and P. esculentus had greater similarity than in
P. lessonae. The P. lessonae displayed the highest number
of correlations between biomarkers and metals. The results
of tissue responses revealed that skin was more susceptible
to metal-induced oxidative stress, with only exception of
As. In the light of these findings, we can suggest the use of
P. esculentus complex species as a biomonitoring species in
studies of metal accumulation and metal-induced oxidative
stress, but with special emphasis on P. lessonae.
PB  - Dordrecht: Springer
T2  - Ecotoxicology
T1  - Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex
IS  - 8
VL  - 25
DO  - 10.1007/s10646-016-1707-x
SP  - 1531
EP  - 1542
ER  - 

@article{
author = "Prokić, Marko and Borković-Mitić, Slavica and Krizmanić, Imre and Mutić, Jelena and Trifković, Jelena and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Pavlović, Slađan and Saičić, Zorica",
year = "2016",
abstract = "Metals are involved in the formation of reactive
oxygen species and can induce oxidative stress. The aim of
this study was to assess the effects of several metals on
oxidative stress in the skin and muscle of the Pelophylax
esculentus “complex” frogs (parental species Pelophylax
ridibundus, Pelophylax lessonae, and their hybrid Pelophylax esculentus) that inhabit the wetland Obedska Bara in
Serbia, and the potential use of these species as bioindicator
organisms in biomonitoring studies. The biomarkers of
oxidative stress (SOD, CAT, GSH-Px, GR, GST activities
and GSH, SH concentrations) and cholinesterase activity
were investigated. The concentrations of nine metals (Fe,
Cu, Zn, As, Cd, Cr, Hg, Ni, and Pb) were measured in the
water and tissues. Correlations were established between
metals and biomarkers in the tissues. The results of metal
accumulation distinguished the skin of P. lessonae and
muscle of P. ridibundus from other P. esculentus complex
species. The oxidative stress biomarkers observed in P.
ridibundus and P. esculentus had greater similarity than in
P. lessonae. The P. lessonae displayed the highest number
of correlations between biomarkers and metals. The results
of tissue responses revealed that skin was more susceptible
to metal-induced oxidative stress, with only exception of
As. In the light of these findings, we can suggest the use of
P. esculentus complex species as a biomonitoring species in
studies of metal accumulation and metal-induced oxidative
stress, but with special emphasis on P. lessonae.",
publisher = "Dordrecht: Springer",
journal = "Ecotoxicology",
title = "Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex",
number = "8",
volume = "25",
doi = "10.1007/s10646-016-1707-x",
pages = "1531-1542"
}

Prokić, M., Borković-Mitić, S., Krizmanić, I., Mutić, J., Trifković, J., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Pavlović, S.,& Saičić, Z.. (2016). Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex. in Ecotoxicology
Dordrecht: Springer., 25(8), 1531-1542.
https://doi.org/10.1007/s10646-016-1707-x

Prokić M, Borković-Mitić S, Krizmanić I, Mutić J, Trifković J, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Pavlović S, Saičić Z. Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex. in Ecotoxicology. 2016;25(8):1531-1542.
doi:10.1007/s10646-016-1707-x .

Prokić, Marko, Borković-Mitić, Slavica, Krizmanić, Imre, Mutić, Jelena, Trifković, Jelena, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Pavlović, Slađan, Saičić, Zorica, "Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex" in Ecotoxicology, 25, no. 8 (2016):1531-1542,
https://doi.org/10.1007/s10646-016-1707-x . .