Proučavanje poremećaja homeostaze i određivanje biomarkera oksidacionog stresa kod aerobnih organizama

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Proučavanje poremećaja homeostaze i određivanje biomarkera oksidacionog stresa kod aerobnih organizama (en)
Проучавање поремећаја хомеостазе и одређивање биомаркера оксидационог стреса код аеробних организама (sr)
Proučavanje poremećaja homeostaze i određivanje biomarkera oksidacionog stresa kod aerobnih organizama (sr_RS)
Authors

Publications

Glutation zavisni enzimi u jetri nekih riba jadranskog mora; sezonske i interspecijske razlike

Pavlović, Slađan; Borković-Mitić, Slavica; Radovanović, Tijana; Gavrilović, Branka; Despotović, Svetlana; Gavrić, Jelena; Milošević, Slaviša; Saičić, Zorica

(Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju, 2011)

TY  - CONF
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Milošević, Slaviša
AU  - Saičić, Zorica
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5940
AB  - Glutation zavisni enzimi imaju značajnu ulogu u održanju ćelijske homeostaze.
Njihova aktivnost može biti indukovana ili inhibirana kao adaptivni odgovor na prirodne
fluktuacije faktora sredine, kao i na izlaganje različitim zagađivačima. Ribe su pogodan
objekat za proučavanje različitih uporedno fizioloških razlika i spoljašnjih uticaja, kao
što su: sezonske varijacije, uticaj ishrane i uticaj zagađivača zato što su široko
rasprostranjene, lako se izlovljavaju i brzo reaguju na promene spoljašnje sredine.
U našoj studiji određivali smo aktivnost glutation zavisnih enzima: glutationperoksidaze (GSH-Px), glutation-reduktaze (GR) i enzima faze II biotransformacije
glutation-S-transferaze (GST) u jetri oslića (Merluccius merluccius L.), trlje (Mullus
barbatus L.) i kokota (Trigla lucerna L.) izlovljavanih na lokalitetu Platamuni
(Jadransko more) tokom zimskog i prolećnog perioda. Cilj istraživanja bio je utvrđivanje
sezonskih razlika u aktivnosti ispitivanih parametara, kao i razlika između ispitivanih
vrsta.
Dobijeni rezultati pokazuju statistički značajno smanjenje aktivnosti GST kod
svih ispitivanih vrsta, kao i GSH-Px u jetri kokota u prolećnom periodu u odnosu na
zimski. U isto vreme, aktivnost GSH-Px bila je značajno povećana u jetri trlje u
prolećnom periodu. Promene aktivnosti GR nisu zabeležene ni kod jedne ispitivane vrste
u obe sezone. Promene aktivnosti između ispitivanih vrsta su konstatovane i u zimskom
i u prolećnom periodu, pri čemu su aktivnosti svih ispitivanih enzima bile statistički
najveće u jetri kokota, zatim trlje, a najmanje u jetri oslića.
Sve dobijene uporedno fiziološke i sezonske razlike se moraju uzeti u obzir
prilikom različitih biomonitoring studija, kao i prilikom procene stepena zagađenja
životne sredine bazirane na molekularno fiziološkim parametrima.
PB  - Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju
C3  - Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
T1  - Glutation zavisni enzimi u jetri nekih riba jadranskog mora; sezonske i interspecijske razlike
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5940
ER  - 
@conference{
author = "Pavlović, Slađan and Borković-Mitić, Slavica and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Milošević, Slaviša and Saičić, Zorica",
year = "2011",
abstract = "Glutation zavisni enzimi imaju značajnu ulogu u održanju ćelijske homeostaze.
Njihova aktivnost može biti indukovana ili inhibirana kao adaptivni odgovor na prirodne
fluktuacije faktora sredine, kao i na izlaganje različitim zagađivačima. Ribe su pogodan
objekat za proučavanje različitih uporedno fizioloških razlika i spoljašnjih uticaja, kao
što su: sezonske varijacije, uticaj ishrane i uticaj zagađivača zato što su široko
rasprostranjene, lako se izlovljavaju i brzo reaguju na promene spoljašnje sredine.
U našoj studiji određivali smo aktivnost glutation zavisnih enzima: glutationperoksidaze (GSH-Px), glutation-reduktaze (GR) i enzima faze II biotransformacije
glutation-S-transferaze (GST) u jetri oslića (Merluccius merluccius L.), trlje (Mullus
barbatus L.) i kokota (Trigla lucerna L.) izlovljavanih na lokalitetu Platamuni
(Jadransko more) tokom zimskog i prolećnog perioda. Cilj istraživanja bio je utvrđivanje
sezonskih razlika u aktivnosti ispitivanih parametara, kao i razlika između ispitivanih
vrsta.
Dobijeni rezultati pokazuju statistički značajno smanjenje aktivnosti GST kod
svih ispitivanih vrsta, kao i GSH-Px u jetri kokota u prolećnom periodu u odnosu na
zimski. U isto vreme, aktivnost GSH-Px bila je značajno povećana u jetri trlje u
prolećnom periodu. Promene aktivnosti GR nisu zabeležene ni kod jedne ispitivane vrste
u obe sezone. Promene aktivnosti između ispitivanih vrsta su konstatovane i u zimskom
i u prolećnom periodu, pri čemu su aktivnosti svih ispitivanih enzima bile statistički
najveće u jetri kokota, zatim trlje, a najmanje u jetri oslića.
Sve dobijene uporedno fiziološke i sezonske razlike se moraju uzeti u obzir
prilikom različitih biomonitoring studija, kao i prilikom procene stepena zagađenja
životne sredine bazirane na molekularno fiziološkim parametrima.",
publisher = "Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju",
journal = "Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia",
title = "Glutation zavisni enzimi u jetri nekih riba jadranskog mora; sezonske i interspecijske razlike",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5940"
}
Pavlović, S., Borković-Mitić, S., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Milošević, S.,& Saičić, Z.. (2011). Glutation zavisni enzimi u jetri nekih riba jadranskog mora; sezonske i interspecijske razlike. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju., 71.
https://hdl.handle.net/21.15107/rcub_ibiss_5940
Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Milošević S, Saičić Z. Glutation zavisni enzimi u jetri nekih riba jadranskog mora; sezonske i interspecijske razlike. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia. 2011;:71.
https://hdl.handle.net/21.15107/rcub_ibiss_5940 .
Pavlović, Slađan, Borković-Mitić, Slavica, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Milošević, Slaviša, Saičić, Zorica, "Glutation zavisni enzimi u jetri nekih riba jadranskog mora; sezonske i interspecijske razlike" in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia (2011):71,
https://hdl.handle.net/21.15107/rcub_ibiss_5940 .

Koncentracija vitamina E i SH grupa u nekim tkivima rečne mrene Barbus barbus iz reke Dunav

Radovanović, Tijana; Gavrilović, Branka; Despotović, Svetlana; Gavrić, Jelena; Borković-Mitić, Slavica; Pavlović, Slađan; Predrag, Cakić; Pajović, Snežana; Saičić, Zorica

(Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju, 2011)

TY  - CONF
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Predrag, Cakić
AU  - Pajović, Snežana
AU  - Saičić, Zorica
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5943
AB  - U našoj studiji određivali smo sezonski zavisnu promenu u koncentraciji
vitamina E i SH grupa u škrgama, bubregu i crevu rečne mrene (Barbus barbus) iz
Dunava, koja predstavlja bentosnu ribu široke geografske distribucije. Istraživanja su
izvedena u prigradskom području Beograda, između Višnjice i Grocke, u prolećnoj i
letnjoj sezoni. Koncentracije ispitivanih parametara merene su standardnim
spektrofotometrijskim metodama.
Rezultati dobijeni za koncentraciju SH grupa u u škrgama, bubregu i crevu
mrene pokazuju statistički značajno povećanje u letnjoj sezoni u poređenju sa prolećnom
sezonom. Koncentracija vitamina E pokazuje statistički značajno smanjenje u letnjoj
sezoni u odnosu na proleće i ovaj trend zabeležen je u svim ispitivanim tkivima. Naša
studija predstavlja prvu procenu koncentracije vitamina E i SH grupa u škrgama,
bubregu i crevu rečne mrene u srpskom delu Dunava.
Zaključuje se da je sezonska fluktuacija ispitivanih komponenti sistema zaštite
od oksidacionih oštećenja u korelaciji sa sezonskim temperaturnim varijacijama.
PB  - Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju
C3  - Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
T1  - Koncentracija vitamina E i SH grupa u nekim tkivima rečne mrene Barbus barbus iz reke Dunav
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5943
ER  - 
@conference{
author = "Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Borković-Mitić, Slavica and Pavlović, Slađan and Predrag, Cakić and Pajović, Snežana and Saičić, Zorica",
year = "2011",
abstract = "U našoj studiji određivali smo sezonski zavisnu promenu u koncentraciji
vitamina E i SH grupa u škrgama, bubregu i crevu rečne mrene (Barbus barbus) iz
Dunava, koja predstavlja bentosnu ribu široke geografske distribucije. Istraživanja su
izvedena u prigradskom području Beograda, između Višnjice i Grocke, u prolećnoj i
letnjoj sezoni. Koncentracije ispitivanih parametara merene su standardnim
spektrofotometrijskim metodama.
Rezultati dobijeni za koncentraciju SH grupa u u škrgama, bubregu i crevu
mrene pokazuju statistički značajno povećanje u letnjoj sezoni u poređenju sa prolećnom
sezonom. Koncentracija vitamina E pokazuje statistički značajno smanjenje u letnjoj
sezoni u odnosu na proleće i ovaj trend zabeležen je u svim ispitivanim tkivima. Naša
studija predstavlja prvu procenu koncentracije vitamina E i SH grupa u škrgama,
bubregu i crevu rečne mrene u srpskom delu Dunava.
Zaključuje se da je sezonska fluktuacija ispitivanih komponenti sistema zaštite
od oksidacionih oštećenja u korelaciji sa sezonskim temperaturnim varijacijama.",
publisher = "Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju",
journal = "Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia",
title = "Koncentracija vitamina E i SH grupa u nekim tkivima rečne mrene Barbus barbus iz reke Dunav",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5943"
}
Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Borković-Mitić, S., Pavlović, S., Predrag, C., Pajović, S.,& Saičić, Z.. (2011). Koncentracija vitamina E i SH grupa u nekim tkivima rečne mrene Barbus barbus iz reke Dunav. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju., 75.
https://hdl.handle.net/21.15107/rcub_ibiss_5943
Radovanović T, Gavrilović B, Despotović S, Gavrić J, Borković-Mitić S, Pavlović S, Predrag C, Pajović S, Saičić Z. Koncentracija vitamina E i SH grupa u nekim tkivima rečne mrene Barbus barbus iz reke Dunav. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia. 2011;:75.
https://hdl.handle.net/21.15107/rcub_ibiss_5943 .
Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Borković-Mitić, Slavica, Pavlović, Slađan, Predrag, Cakić, Pajović, Snežana, Saičić, Zorica, "Koncentracija vitamina E i SH grupa u nekim tkivima rečne mrene Barbus barbus iz reke Dunav" in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia (2011):75,
https://hdl.handle.net/21.15107/rcub_ibiss_5943 .

Biomarkeri oksidacionog stresa u nekim tkivima slikarske školjke (Unio pictorum) iz Dunava i Tise

Gavrić, Jelena; Despotović, Svetlana; Gavrilović, Branka; Radovanović, Tijana; Borković-Mitić, Slavica; Pavlović, Slađan; Paunović, Momir; Saičić, Zorica

(Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju, 2011)

TY  - CONF
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Paunović, Momir
AU  - Saičić, Zorica
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5939
AB  - Biomarkeri oksidacionog stresa predstavljaju značajne pokazatelje
oksidacionog stresa uzrokovanog raznim zagađivačima, kao i drugim promenama u
životnoj sredini. Povećanje ili smanjenje aktivnosti enzima sistema zaštite od
oksidacionih oštećenja dovodi do narušavanja prooksidaciono/antioksidacione ravnoteže
i može da bude značajan biomarker štetnog uticaja različitih sredinskih faktora.
U našoj studiji određivali smo aktivnosti biomarkera oksidacionog stresa:
superoksid-dismutaze (SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px),
glutation-raduktaze (GR) i enzima faze II biotransformacije glutation-S-transferaze
(GST) u stopalu, škrgama i visceralnoj masi slikarske školjke Unio pictorum, izlovljene
na rekama Dunav (lokalitet Orešac) i Tisa (lokalitet Ada). Takođe, određivan je i sadržaj
glutationa (GSH) u tkivu stopala.
Dobijeni rezultati pokazuju da su aktivnosti CAT, GSH-Px i GST statistički
značajno povećane u svim ispitivanim tkivima školjki iz reke Tise u odnosu na Dunav.
Aktivnost SOD bila je statistički značajno povećana samo u stopalu na reci Tisi. Nije
zabeležena promena u aktivnosti GR ni u jednom od ispitivanih tkiva školjki iz dve reke.
Koncentracija GSH u stopalu značajno je veća u školjkama iz Tise u odnosu na Dunav.
Na osnovu dobijenih rezultata može se zaključiti da je povećanje vrednosti
biomarkera oksidacionog stresa u svim ispitivanim tkivima školjke U. pictorum iz reke
Tise u odnosu na Dunav fiziološki odgovor organizma na razlike u sredinskim faktorima
ova dva rečna ekosistema, posebno na prisustvo različitih koncentracija zagađivača u
reci Tisi. Takođe smo konstatovali da se CAT, GSH-Px i GST, kao i GSH karakterišu
specifinim ćelijskim odgovorom na ekosistemske promene. Karakteristike školjki kao
što su sedentarni način života, ishrana filtracijom, mogućnost akumulacije i
koncentracije polutanata čine ih veoma pogodnim bioindikatorima za molekularno
fiziološki biomonitoring.
PB  - Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju
C3  - Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
T1  - Biomarkeri oksidacionog stresa u nekim tkivima slikarske školjke (Unio pictorum) iz Dunava i Tise
SP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5939
ER  - 
@conference{
author = "Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Borković-Mitić, Slavica and Pavlović, Slađan and Paunović, Momir and Saičić, Zorica",
year = "2011",
abstract = "Biomarkeri oksidacionog stresa predstavljaju značajne pokazatelje
oksidacionog stresa uzrokovanog raznim zagađivačima, kao i drugim promenama u
životnoj sredini. Povećanje ili smanjenje aktivnosti enzima sistema zaštite od
oksidacionih oštećenja dovodi do narušavanja prooksidaciono/antioksidacione ravnoteže
i može da bude značajan biomarker štetnog uticaja različitih sredinskih faktora.
U našoj studiji određivali smo aktivnosti biomarkera oksidacionog stresa:
superoksid-dismutaze (SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px),
glutation-raduktaze (GR) i enzima faze II biotransformacije glutation-S-transferaze
(GST) u stopalu, škrgama i visceralnoj masi slikarske školjke Unio pictorum, izlovljene
na rekama Dunav (lokalitet Orešac) i Tisa (lokalitet Ada). Takođe, određivan je i sadržaj
glutationa (GSH) u tkivu stopala.
Dobijeni rezultati pokazuju da su aktivnosti CAT, GSH-Px i GST statistički
značajno povećane u svim ispitivanim tkivima školjki iz reke Tise u odnosu na Dunav.
Aktivnost SOD bila je statistički značajno povećana samo u stopalu na reci Tisi. Nije
zabeležena promena u aktivnosti GR ni u jednom od ispitivanih tkiva školjki iz dve reke.
Koncentracija GSH u stopalu značajno je veća u školjkama iz Tise u odnosu na Dunav.
Na osnovu dobijenih rezultata može se zaključiti da je povećanje vrednosti
biomarkera oksidacionog stresa u svim ispitivanim tkivima školjke U. pictorum iz reke
Tise u odnosu na Dunav fiziološki odgovor organizma na razlike u sredinskim faktorima
ova dva rečna ekosistema, posebno na prisustvo različitih koncentracija zagađivača u
reci Tisi. Takođe smo konstatovali da se CAT, GSH-Px i GST, kao i GSH karakterišu
specifinim ćelijskim odgovorom na ekosistemske promene. Karakteristike školjki kao
što su sedentarni način života, ishrana filtracijom, mogućnost akumulacije i
koncentracije polutanata čine ih veoma pogodnim bioindikatorima za molekularno
fiziološki biomonitoring.",
publisher = "Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju",
journal = "Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia",
title = "Biomarkeri oksidacionog stresa u nekim tkivima slikarske školjke (Unio pictorum) iz Dunava i Tise",
pages = "52",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5939"
}
Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Borković-Mitić, S., Pavlović, S., Paunović, M.,& Saičić, Z.. (2011). Biomarkeri oksidacionog stresa u nekim tkivima slikarske školjke (Unio pictorum) iz Dunava i Tise. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju., 52.
https://hdl.handle.net/21.15107/rcub_ibiss_5939
Gavrić J, Despotović S, Gavrilović B, Radovanović T, Borković-Mitić S, Pavlović S, Paunović M, Saičić Z. Biomarkeri oksidacionog stresa u nekim tkivima slikarske školjke (Unio pictorum) iz Dunava i Tise. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia. 2011;:52.
https://hdl.handle.net/21.15107/rcub_ibiss_5939 .
Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Borković-Mitić, Slavica, Pavlović, Slađan, Paunović, Momir, Saičić, Zorica, "Biomarkeri oksidacionog stresa u nekim tkivima slikarske školjke (Unio pictorum) iz Dunava i Tise" in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia (2011):52,
https://hdl.handle.net/21.15107/rcub_ibiss_5939 .

Biohemijski biomarkeri oksidacionog stresa kod rečnog puža (Viviparus acerosus) iz Velike Morave

Despotović, Svetlana; Gavrilović, Branka; Gavrić, Jelena; Radovanović, Tijana; Borković-Mitić, Slavica; Pavlović, Slađan; Paunović, Momir; Saičić, Zorica

(Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju, 2011)

TY  - CONF
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Paunović, Momir
AU  - Saičić, Zorica
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5942
AB  - U našoj studiji koristili smo jedinke rečnog puža Viviparus acerosus sakupljene
na lokalitetu Varvarin na Velikoj Moravi i jedinke iste vrste i sa istog lokaliteta koje su
mesec dana pre eksperimenta adaptirane na akvarijumske uslove i koje predstavljaju
kontrole. Određivali smo aktivnost biohemijskih biomarkera oksidacionog stresa:
superoksid-dismutaze (SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px) i
glutation-reduktaze (GR), enzima faze II biotransformacije glutation-S-transferaze
(GST), kao i koncentraciju glutationa (GSH) kod puževa V.acerosus.
Dobijeni rezultati pokazuju da su aktivnosti CAT, GSH-Px, GR i GST, kao i
koncentracija GSH bile statistički značajno veće kod puževa V. acerosus sakupljenih na
Velikoj Moravi u poređenju sa kontrolama. Nisu zabeležene statistički značajne razlike
u aktivnosti SOD između dve ispitivane grupe puževa.
Dobijeni rezultati ukazuju na povećani oksidacioni stres kod puževa V. acerosus
iz Velike Morave u odnosu na kontrolne, akvarijumske jedinke. U prirodnim uslovima
na akvatične organizme deluju složeni abiotički i biotički faktori i povećan je stepen
antropogenog uticaja, čime se objašnjavaju dobijene statističke razlike biohemijskih
biomarkera oksidacionog stresa u našoj studiji. Da bi se dobila bolja slika o stanju
životne sredine, u molekularno fiziološki biomonitoring značajno je uključiti i kontrolne
jedinke adaptirane na akvarijumske uslove.
PB  - Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju
C3  - Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
T1  - Biohemijski biomarkeri oksidacionog stresa kod rečnog puža (Viviparus acerosus) iz Velike Morave
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5942
ER  - 
@conference{
author = "Despotović, Svetlana and Gavrilović, Branka and Gavrić, Jelena and Radovanović, Tijana and Borković-Mitić, Slavica and Pavlović, Slađan and Paunović, Momir and Saičić, Zorica",
year = "2011",
abstract = "U našoj studiji koristili smo jedinke rečnog puža Viviparus acerosus sakupljene
na lokalitetu Varvarin na Velikoj Moravi i jedinke iste vrste i sa istog lokaliteta koje su
mesec dana pre eksperimenta adaptirane na akvarijumske uslove i koje predstavljaju
kontrole. Određivali smo aktivnost biohemijskih biomarkera oksidacionog stresa:
superoksid-dismutaze (SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px) i
glutation-reduktaze (GR), enzima faze II biotransformacije glutation-S-transferaze
(GST), kao i koncentraciju glutationa (GSH) kod puževa V.acerosus.
Dobijeni rezultati pokazuju da su aktivnosti CAT, GSH-Px, GR i GST, kao i
koncentracija GSH bile statistički značajno veće kod puževa V. acerosus sakupljenih na
Velikoj Moravi u poređenju sa kontrolama. Nisu zabeležene statistički značajne razlike
u aktivnosti SOD između dve ispitivane grupe puževa.
Dobijeni rezultati ukazuju na povećani oksidacioni stres kod puževa V. acerosus
iz Velike Morave u odnosu na kontrolne, akvarijumske jedinke. U prirodnim uslovima
na akvatične organizme deluju složeni abiotički i biotički faktori i povećan je stepen
antropogenog uticaja, čime se objašnjavaju dobijene statističke razlike biohemijskih
biomarkera oksidacionog stresa u našoj studiji. Da bi se dobila bolja slika o stanju
životne sredine, u molekularno fiziološki biomonitoring značajno je uključiti i kontrolne
jedinke adaptirane na akvarijumske uslove.",
publisher = "Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju",
journal = "Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia",
title = "Biohemijski biomarkeri oksidacionog stresa kod rečnog puža (Viviparus acerosus) iz Velike Morave",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5942"
}
Despotović, S., Gavrilović, B., Gavrić, J., Radovanović, T., Borković-Mitić, S., Pavlović, S., Paunović, M.,& Saičić, Z.. (2011). Biohemijski biomarkeri oksidacionog stresa kod rečnog puža (Viviparus acerosus) iz Velike Morave. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_5942
Despotović S, Gavrilović B, Gavrić J, Radovanović T, Borković-Mitić S, Pavlović S, Paunović M, Saičić Z. Biohemijski biomarkeri oksidacionog stresa kod rečnog puža (Viviparus acerosus) iz Velike Morave. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia. 2011;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_5942 .
Despotović, Svetlana, Gavrilović, Branka, Gavrić, Jelena, Radovanović, Tijana, Borković-Mitić, Slavica, Pavlović, Slađan, Paunović, Momir, Saičić, Zorica, "Biohemijski biomarkeri oksidacionog stresa kod rečnog puža (Viviparus acerosus) iz Velike Morave" in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia (2011):73,
https://hdl.handle.net/21.15107/rcub_ibiss_5942 .

Biomarkeri oksidacionog stresa u različitim tkivima Slatkovodnih školjki

Borković-Mitić, Slavica; Pavlović, Slađan; Radovanović, Tijana; Gavrilović, Branka; Despotović, Svetlana; Gavrić, Jelena; Saičić, Zorica

(Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju, 2011)

TY  - CONF
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Saičić, Zorica
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5937
AB  - U nаšoj studiji ispitivali smo biomarkere oksidacionog stresa - antioksidacione
zaštitne enzime: superoksid-dismutazu (SOD), katalazu (CAT), glutation-peroksidazu
(GSH-Px), glutation-reduktazu (GR), kao i enzim faze II biotransformacije glutation-Stransferazu (GST) u digestivnoj žlezdi, škrgаmа i stopalu kod slаtkovodnih školjki Unio
tumidus, Unio pictorum i Sinanodota woodiana nа četiri lokаlitetа (Jаmenа, Sremskа
Mitrovicа, Šаbаc i Ostružnicа) nа reci Sаvi.
Rezultаti pokаzuju dа svi ispitivani biomarkeri oksidacionog stresa, kаo i
elektroforetskа аnаlizа SOD kod slаtkovodnih školjki mogu dа budu koristаn pаrаmetаr
u biomonitoringu. Elektroforetskom аnаlizom SOD u tkivima ispitivanih školjki
dobijena je trećа trаkа kojа predstаvljа jedinstvenu izoformu SOD-3 kojа se pojаvljuje
sаmo u zаgаđenim uslovimа sredine. Uz pomoć Spirman-ovih korelаcijа pokаzаno je dа
se аktivnost SOD rаzlikuje nа lokаlitetimа što potvrđuje dа se promenа аktivnosti SOD
može koristiti kаo pouzdаn biomаrker. Promene аktivnosti CAT i GSH-Px su tkivno
specifične. Aktivnost GR rаzlikuje se u odnosu nа lokаlitete i tkivа, tаko dа i promenа
аktivnosti GR može biti biomаrker, аko se uzmu u obzir tkivne specifičnosti. Aktivnost
GST rаzlikuje se po vrstama, lokаlitetu i tkivu, što znači dа GST može biti dobar
biomаrker, аko se prilikom аnаlize uzmu u obzir i pomenute kаrаkteristike.
Nаša istraživanja predstаvljаju prvi obimаn izveštаj o promeni aktivnosti
antioksidacionih zaštitnih enzima kao relevаntnih biohemijskih biomаrkera
oksidаcionog stresа kod slаtkovodnih školjki U. tumidus, U. pictorum i S. woodiana nа
reci Savi. Svi ispitivani pаrаmetri mogu se koristiti kаo efektivni biomаrkeri zа
biomonitoring spoljаšnje sredine. S obzirom dа školjke predstаvljаju jednu od vаžnih
komponenti zаjednice u velikim rаvničаrskim rekаmа Srbije, prikаzаnа metodologijа je
primenjivа zа rutinski molekularno fiziološki biomonitoring ovih ekosistemа.
PB  - Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju
C3  - Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
T1  - Biomarkeri oksidacionog stresa u različitim tkivima Slatkovodnih školjki
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5937
ER  - 
@conference{
author = "Borković-Mitić, Slavica and Pavlović, Slađan and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Saičić, Zorica",
year = "2011",
abstract = "U nаšoj studiji ispitivali smo biomarkere oksidacionog stresa - antioksidacione
zaštitne enzime: superoksid-dismutazu (SOD), katalazu (CAT), glutation-peroksidazu
(GSH-Px), glutation-reduktazu (GR), kao i enzim faze II biotransformacije glutation-Stransferazu (GST) u digestivnoj žlezdi, škrgаmа i stopalu kod slаtkovodnih školjki Unio
tumidus, Unio pictorum i Sinanodota woodiana nа četiri lokаlitetа (Jаmenа, Sremskа
Mitrovicа, Šаbаc i Ostružnicа) nа reci Sаvi.
Rezultаti pokаzuju dа svi ispitivani biomarkeri oksidacionog stresa, kаo i
elektroforetskа аnаlizа SOD kod slаtkovodnih školjki mogu dа budu koristаn pаrаmetаr
u biomonitoringu. Elektroforetskom аnаlizom SOD u tkivima ispitivanih školjki
dobijena je trećа trаkа kojа predstаvljа jedinstvenu izoformu SOD-3 kojа se pojаvljuje
sаmo u zаgаđenim uslovimа sredine. Uz pomoć Spirman-ovih korelаcijа pokаzаno je dа
se аktivnost SOD rаzlikuje nа lokаlitetimа što potvrđuje dа se promenа аktivnosti SOD
može koristiti kаo pouzdаn biomаrker. Promene аktivnosti CAT i GSH-Px su tkivno
specifične. Aktivnost GR rаzlikuje se u odnosu nа lokаlitete i tkivа, tаko dа i promenа
аktivnosti GR može biti biomаrker, аko se uzmu u obzir tkivne specifičnosti. Aktivnost
GST rаzlikuje se po vrstama, lokаlitetu i tkivu, što znači dа GST može biti dobar
biomаrker, аko se prilikom аnаlize uzmu u obzir i pomenute kаrаkteristike.
Nаša istraživanja predstаvljаju prvi obimаn izveštаj o promeni aktivnosti
antioksidacionih zaštitnih enzima kao relevаntnih biohemijskih biomаrkera
oksidаcionog stresа kod slаtkovodnih školjki U. tumidus, U. pictorum i S. woodiana nа
reci Savi. Svi ispitivani pаrаmetri mogu se koristiti kаo efektivni biomаrkeri zа
biomonitoring spoljаšnje sredine. S obzirom dа školjke predstаvljаju jednu od vаžnih
komponenti zаjednice u velikim rаvničаrskim rekаmа Srbije, prikаzаnа metodologijа je
primenjivа zа rutinski molekularno fiziološki biomonitoring ovih ekosistemа.",
publisher = "Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju",
journal = "Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia",
title = "Biomarkeri oksidacionog stresa u različitim tkivima Slatkovodnih školjki",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5937"
}
Borković-Mitić, S., Pavlović, S., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J.,& Saičić, Z.. (2011). Biomarkeri oksidacionog stresa u različitim tkivima Slatkovodnih školjki. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju., 30.
https://hdl.handle.net/21.15107/rcub_ibiss_5937
Borković-Mitić S, Pavlović S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Saičić Z. Biomarkeri oksidacionog stresa u različitim tkivima Slatkovodnih školjki. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia. 2011;:30.
https://hdl.handle.net/21.15107/rcub_ibiss_5937 .
Borković-Mitić, Slavica, Pavlović, Slađan, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Saičić, Zorica, "Biomarkeri oksidacionog stresa u različitim tkivima Slatkovodnih školjki" in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia (2011):30,
https://hdl.handle.net/21.15107/rcub_ibiss_5937 .

Promene aktivnosti superoksid-dismutaze i katalaze u nekim tkivima slatkovodne ribe grgeča (Perca fluviatilis) kao odgovor na cvetanje cijanobakterija u gružanskom jezeru

Gavrilović, Branka; Despotović, Svetlana; Gavrić, Jelena; Radovanović, Tijana; Borković-Mitić, Slavica; Pavlović, Slađan; Simić, Snežana; Ognjanović, Branka; Pajović, Snežana; Saičić, Zorica

(Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju, 2011)

TY  - CONF
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Simić, Snežana
AU  - Ognjanović, Branka
AU  - Pajović, Snežana
AU  - Saičić, Zorica
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5938
AB  - U našoj studiji istraživali smo promene aktivnosti enzima zaštite od oksidacionih oštećenja: ukupne superoksid-dismutaze (Uk SOD), bakar, cink sadržavajuće superoksid-dismutaze (CuZnSOD), mangan sadržavajuće superoksiddismutaze (MnSOD) i katalaze (CAT) u škrgama i mišiću slatkovodne ribe grgeča (Perca fluviatilis) kao odgovor na cvetanje cijanobakterije Aphanizomenon flos-aquae u Gružanskom jezeru. Takođe, ispitivana je koncentracija ukupnih proteina, kao i elektroforetska analiza proteina i SOD. Svi parametri određivani su tokom dva perioda, pre i za vreme cvetanja. Aktivnosti Uk SOD i MnSOD bile su niže i u škrgama i u mišiću, dok je aktivnost CuZnSOD pokazala niže vrednosti u mišiću za vreme cvetanja u odnosu na period pre cvetanja. Aktivnost CAT bila je značajno veća u oba ispitivana tkiva tokom cvetanja. U mišiću je zabeleženo statistički značajno povećanje koncentracije ukupnih proteina za vreme cvetanja, dok je elektroforetska analiza proteina ukazala na razlike u profilu proteina u škrgama i mišiću između dva ispitivana perioda. Elektroforetski profil enzima SOD pokazao je tipičan distribucioni obrazac SOD izoformi u oba tkiva. Naš rad predstavlja prvu studiju o uticaju cvetanja cijanobakterija na aktivnost enzima zaštite od oksidacionih oštećenja superoksid-dismutaze i katalaze u škrgama i mišiću slatkovodne ribe grgeča (Perca fluviatilis) iz Gružanskog jezera i ukazuje da cvetanje Aphanizomenon flos-aquae indukuje oksidacioni stres u ispitivanim tkivima. Ovo istraživanje daje osnovu za molekularno fiziološki biomonitoring uticaja cijanobakterija na različite slatkovodne organizme.
PB  - Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju
C3  - Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
T1  - Promene aktivnosti superoksid-dismutaze i katalaze u nekim tkivima slatkovodne ribe grgeča (Perca fluviatilis) kao odgovor na cvetanje cijanobakterija u gružanskom jezeru
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5938
ER  - 
@conference{
author = "Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Radovanović, Tijana and Borković-Mitić, Slavica and Pavlović, Slađan and Simić, Snežana and Ognjanović, Branka and Pajović, Snežana and Saičić, Zorica",
year = "2011",
abstract = "U našoj studiji istraživali smo promene aktivnosti enzima zaštite od oksidacionih oštećenja: ukupne superoksid-dismutaze (Uk SOD), bakar, cink sadržavajuće superoksid-dismutaze (CuZnSOD), mangan sadržavajuće superoksiddismutaze (MnSOD) i katalaze (CAT) u škrgama i mišiću slatkovodne ribe grgeča (Perca fluviatilis) kao odgovor na cvetanje cijanobakterije Aphanizomenon flos-aquae u Gružanskom jezeru. Takođe, ispitivana je koncentracija ukupnih proteina, kao i elektroforetska analiza proteina i SOD. Svi parametri određivani su tokom dva perioda, pre i za vreme cvetanja. Aktivnosti Uk SOD i MnSOD bile su niže i u škrgama i u mišiću, dok je aktivnost CuZnSOD pokazala niže vrednosti u mišiću za vreme cvetanja u odnosu na period pre cvetanja. Aktivnost CAT bila je značajno veća u oba ispitivana tkiva tokom cvetanja. U mišiću je zabeleženo statistički značajno povećanje koncentracije ukupnih proteina za vreme cvetanja, dok je elektroforetska analiza proteina ukazala na razlike u profilu proteina u škrgama i mišiću između dva ispitivana perioda. Elektroforetski profil enzima SOD pokazao je tipičan distribucioni obrazac SOD izoformi u oba tkiva. Naš rad predstavlja prvu studiju o uticaju cvetanja cijanobakterija na aktivnost enzima zaštite od oksidacionih oštećenja superoksid-dismutaze i katalaze u škrgama i mišiću slatkovodne ribe grgeča (Perca fluviatilis) iz Gružanskog jezera i ukazuje da cvetanje Aphanizomenon flos-aquae indukuje oksidacioni stres u ispitivanim tkivima. Ovo istraživanje daje osnovu za molekularno fiziološki biomonitoring uticaja cijanobakterija na različite slatkovodne organizme.",
publisher = "Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju",
journal = "Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia",
title = "Promene aktivnosti superoksid-dismutaze i katalaze u nekim tkivima slatkovodne ribe grgeča (Perca fluviatilis) kao odgovor na cvetanje cijanobakterija u gružanskom jezeru",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5938"
}
Gavrilović, B., Despotović, S., Gavrić, J., Radovanović, T., Borković-Mitić, S., Pavlović, S., Simić, S., Ognjanović, B., Pajović, S.,& Saičić, Z.. (2011). Promene aktivnosti superoksid-dismutaze i katalaze u nekim tkivima slatkovodne ribe grgeča (Perca fluviatilis) kao odgovor na cvetanje cijanobakterija u gružanskom jezeru. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia
Beograd: Srpsko društvo za mitohondrijalnu i slobodno-radikalsku fiziologiju., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_5938
Gavrilović B, Despotović S, Gavrić J, Radovanović T, Borković-Mitić S, Pavlović S, Simić S, Ognjanović B, Pajović S, Saičić Z. Promene aktivnosti superoksid-dismutaze i katalaze u nekim tkivima slatkovodne ribe grgeča (Perca fluviatilis) kao odgovor na cvetanje cijanobakterija u gružanskom jezeru. in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia. 2011;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_5938 .
Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Radovanović, Tijana, Borković-Mitić, Slavica, Pavlović, Slađan, Simić, Snežana, Ognjanović, Branka, Pajović, Snežana, Saičić, Zorica, "Promene aktivnosti superoksid-dismutaze i katalaze u nekim tkivima slatkovodne ribe grgeča (Perca fluviatilis) kao odgovor na cvetanje cijanobakterija u gružanskom jezeru" in Knjiga sažetaka: Prvi Kongres Mitohondrije i slobodni radikala - perspektive; 2011 Sep 24; Belgrade, Serbia (2011):41,
https://hdl.handle.net/21.15107/rcub_ibiss_5938 .

Superoksid-dismutaza kao relevantan biomarker oksidacionog stresa kod slatkovodne školjke Unio pictorum na različitim lokalitetima-primer istraživanja reke Save

Borković-Mitić, Slavica; Radovanović, Tijana; Gavrilović, Branka; Despotović, Svetlana; Pavlović, Slađan; Saičić, Zorica

(Belgrade : Republic of Serbia, Ministry of agriculture, forestry and water management - Republic directorate of water, 2010)

TY  - CHAP
AU  - Borković-Mitić, Slavica
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6025
AB  - The activity of superoxide dismutase (SOD), total protein as well as protein and SOD electrophoretic profiles in the digestive gland and the gills of the freshwater bivalve Unio tumidus at four localities of the River Sava (Jamena, Sremska Mitrovica, Šabac and Ostružnica) was examined. The specific SOD activity in the digestive gland of the U. tumidus was significantly higher in Jamena in respect to Šabac and Ostružnica. The different trend was obtained for the total SOD activity in the digestive gland, which was significantly increased in Sremska Mitrovica compared to investigated localities Šabac and Ostružnica. The total SOD activity in the digestive gland was also significantly higher in Jamena compared to Šabac, as well as in Šabac in respect to Ostružnica. The activity of total SOD in the gills of U. tumidus was significantly higher in Sremska Mitrovica in respect to Jamena and Šabac. The results obtained in our study show strong tissue and locality specificities, indicating different metabolic activities and various environmental influences. Our work also suggests, that the variations of SOD expression pattern in Unio tumidus could be used as a tool for the environment monitoring. Our study represents the first comprehensive report on SOD as a relevant oxidative stress biomarker in the freshwater bivalve Unio tumidus at different localities  from the Sava River and provide the basis for future studies that will consider our reported parameters as potential biomarkers for biomonitoring of basal environmental conditions and some anthropogenic impacts.
AB  - Испитивана је активност супероксид дисмутазе (СОД), укупниџ протеина, као и електрофоретских профила протеина СОД у дигестивној жлезди и шкргама слатководне шкољке Unio tumidus
на четири локалитета реке Саве (Јамена, Сремска Митровица, Шабац и Остружница). Специфична активност СОД у дигестивној жлезди U. tumidus била је значајно већа у Јамени у односу на Шабац и Остружницу. Добијен је другачији тренд за укупну активност СОД у дигестивној жлезди, која је значајно повећана у Сремској Митровици у поређењу са истраживаним локалитетима Шабац и Остружница. Укупна активност СОД у дигестивној жлезди такође је била значајно већа у Јамени у односу на Шабац, као и и у Шапцу у односу на Остружницу. Активност укупне СОД у шкргама U. tumidus била је значајно већа у Сремској Митровици у односу на Јамену и Шабац. Резултати добијени у нашој студији показују снажне специфичности ткива и локалитета, што указује на различите метаболичке активности и различите утицаје околине. Наш рад такође сугерише да се варијације обрасца експресије СОД у Unio tumidus могу користити као алат за праћење животне средине. Наша студија представља први свеобухватан извештај о СОД као релевантном биомаркеру оксидативног стреса у слатководној шкољки Unio tumidus на различитим локалитетима од реке Саве и пружа основу за будућа истраживања која ће разматрати наше пријављене параметре као потенцијалне биомаркере за биомониторинг базалних услова животне средине. и неки антропогени утицаји.
AB  - Ispitivana je aktivnost superoksid dismutaze (SOD), ukupnidž proteina, kao i elektroforetskih profila proteina SOD u digestivnoj žlezdi i škrgama slatkovodne školjke Unio tumidus na četiri lokaliteta reke Save (Jamena, Sremska Mitrovica, Šabac i Ostružnica). Specifična aktivnost SOD u digestivnoj žlezdi U. tumidus bila je značajno veća u Jameni u odnosu na Šabac i Ostružnicu. Dobijen je drugačiji trend za ukupnu aktivnost SOD u digestivnoj žlezdi, koja je značajno povećana u Sremskoj Mitrovici u poređenju sa istraživanim lokalitetima Šabac i Ostružnica. Ukupna aktivnost SOD u digestivnoj žlezdi takođe je bila značajno veća u Jameni u odnosu na Šabac, kao i i u Šapcu u odnosu na Ostružnicu. Aktivnost ukupne SOD u škrgama U. tumidus bila je značajno veća u Sremskoj Mitrovici u odnosu na Jamenu i Šabac. Rezultati dobijeni u našoj studiji pokazuju snažne specifičnosti tkiva i lokaliteta, što ukazuje na različite metaboličke aktivnosti i različite uticaje okoline. Naš rad takođe sugeriše da se varijacije obrasca ekspresije SOD u Unio tumidus mogu koristiti kao alat za praćenje životne sredine. Naša studija predstavlja prvi sveobuhvatan izveštaj o SOD kao relevantnom biomarkeru oksidativnog stresa u slatkovodnoj školjki Unio tumidus na različitim lokalitetima od reke Save i pruža osnovu za buduća istraživanja koja će razmatrati naše prijavljene parametre kao potencijalne biomarkere za biomonitoring bazalnih uslova životne sredine. i neki antropogeni uticaji.
PB  - Belgrade : Republic of Serbia, Ministry of agriculture, forestry and water management - Republic directorate of water
T2  - The Danube in Serbia : the results of national program of the Second joint Danube survey 2007
T1  - Superoksid-dismutaza kao relevantan biomarker oksidacionog stresa kod slatkovodne školjke Unio pictorum na različitim lokalitetima-primer istraživanja reke Save
T1  - Superoxide dismutase as relevant oxidative stress biomarker of the freshwater bivalve Unio tumidus at different localities - the Sava river case study
SP  - 225
EP  - 240
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6025
ER  - 
@inbook{
author = "Borković-Mitić, Slavica and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Pavlović, Slađan and Saičić, Zorica",
year = "2010",
abstract = "The activity of superoxide dismutase (SOD), total protein as well as protein and SOD electrophoretic profiles in the digestive gland and the gills of the freshwater bivalve Unio tumidus at four localities of the River Sava (Jamena, Sremska Mitrovica, Šabac and Ostružnica) was examined. The specific SOD activity in the digestive gland of the U. tumidus was significantly higher in Jamena in respect to Šabac and Ostružnica. The different trend was obtained for the total SOD activity in the digestive gland, which was significantly increased in Sremska Mitrovica compared to investigated localities Šabac and Ostružnica. The total SOD activity in the digestive gland was also significantly higher in Jamena compared to Šabac, as well as in Šabac in respect to Ostružnica. The activity of total SOD in the gills of U. tumidus was significantly higher in Sremska Mitrovica in respect to Jamena and Šabac. The results obtained in our study show strong tissue and locality specificities, indicating different metabolic activities and various environmental influences. Our work also suggests, that the variations of SOD expression pattern in Unio tumidus could be used as a tool for the environment monitoring. Our study represents the first comprehensive report on SOD as a relevant oxidative stress biomarker in the freshwater bivalve Unio tumidus at different localities  from the Sava River and provide the basis for future studies that will consider our reported parameters as potential biomarkers for biomonitoring of basal environmental conditions and some anthropogenic impacts., Испитивана је активност супероксид дисмутазе (СОД), укупниџ протеина, као и електрофоретских профила протеина СОД у дигестивној жлезди и шкргама слатководне шкољке Unio tumidus
на четири локалитета реке Саве (Јамена, Сремска Митровица, Шабац и Остружница). Специфична активност СОД у дигестивној жлезди U. tumidus била је значајно већа у Јамени у односу на Шабац и Остружницу. Добијен је другачији тренд за укупну активност СОД у дигестивној жлезди, која је значајно повећана у Сремској Митровици у поређењу са истраживаним локалитетима Шабац и Остружница. Укупна активност СОД у дигестивној жлезди такође је била значајно већа у Јамени у односу на Шабац, као и и у Шапцу у односу на Остружницу. Активност укупне СОД у шкргама U. tumidus била је значајно већа у Сремској Митровици у односу на Јамену и Шабац. Резултати добијени у нашој студији показују снажне специфичности ткива и локалитета, што указује на различите метаболичке активности и различите утицаје околине. Наш рад такође сугерише да се варијације обрасца експресије СОД у Unio tumidus могу користити као алат за праћење животне средине. Наша студија представља први свеобухватан извештај о СОД као релевантном биомаркеру оксидативног стреса у слатководној шкољки Unio tumidus на различитим локалитетима од реке Саве и пружа основу за будућа истраживања која ће разматрати наше пријављене параметре као потенцијалне биомаркере за биомониторинг базалних услова животне средине. и неки антропогени утицаји., Ispitivana je aktivnost superoksid dismutaze (SOD), ukupnidž proteina, kao i elektroforetskih profila proteina SOD u digestivnoj žlezdi i škrgama slatkovodne školjke Unio tumidus na četiri lokaliteta reke Save (Jamena, Sremska Mitrovica, Šabac i Ostružnica). Specifična aktivnost SOD u digestivnoj žlezdi U. tumidus bila je značajno veća u Jameni u odnosu na Šabac i Ostružnicu. Dobijen je drugačiji trend za ukupnu aktivnost SOD u digestivnoj žlezdi, koja je značajno povećana u Sremskoj Mitrovici u poređenju sa istraživanim lokalitetima Šabac i Ostružnica. Ukupna aktivnost SOD u digestivnoj žlezdi takođe je bila značajno veća u Jameni u odnosu na Šabac, kao i i u Šapcu u odnosu na Ostružnicu. Aktivnost ukupne SOD u škrgama U. tumidus bila je značajno veća u Sremskoj Mitrovici u odnosu na Jamenu i Šabac. Rezultati dobijeni u našoj studiji pokazuju snažne specifičnosti tkiva i lokaliteta, što ukazuje na različite metaboličke aktivnosti i različite uticaje okoline. Naš rad takođe sugeriše da se varijacije obrasca ekspresije SOD u Unio tumidus mogu koristiti kao alat za praćenje životne sredine. Naša studija predstavlja prvi sveobuhvatan izveštaj o SOD kao relevantnom biomarkeru oksidativnog stresa u slatkovodnoj školjki Unio tumidus na različitim lokalitetima od reke Save i pruža osnovu za buduća istraživanja koja će razmatrati naše prijavljene parametre kao potencijalne biomarkere za biomonitoring bazalnih uslova životne sredine. i neki antropogeni uticaji.",
publisher = "Belgrade : Republic of Serbia, Ministry of agriculture, forestry and water management - Republic directorate of water",
journal = "The Danube in Serbia : the results of national program of the Second joint Danube survey 2007",
booktitle = "Superoksid-dismutaza kao relevantan biomarker oksidacionog stresa kod slatkovodne školjke Unio pictorum na različitim lokalitetima-primer istraživanja reke Save, Superoxide dismutase as relevant oxidative stress biomarker of the freshwater bivalve Unio tumidus at different localities - the Sava river case study",
pages = "225-240",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6025"
}
Borković-Mitić, S., Radovanović, T., Gavrilović, B., Despotović, S., Pavlović, S.,& Saičić, Z.. (2010). Superoksid-dismutaza kao relevantan biomarker oksidacionog stresa kod slatkovodne školjke Unio pictorum na različitim lokalitetima-primer istraživanja reke Save. in The Danube in Serbia : the results of national program of the Second joint Danube survey 2007
Belgrade : Republic of Serbia, Ministry of agriculture, forestry and water management - Republic directorate of water., 225-240.
https://hdl.handle.net/21.15107/rcub_ibiss_6025
Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Pavlović S, Saičić Z. Superoksid-dismutaza kao relevantan biomarker oksidacionog stresa kod slatkovodne školjke Unio pictorum na različitim lokalitetima-primer istraživanja reke Save. in The Danube in Serbia : the results of national program of the Second joint Danube survey 2007. 2010;:225-240.
https://hdl.handle.net/21.15107/rcub_ibiss_6025 .
Borković-Mitić, Slavica, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Pavlović, Slađan, Saičić, Zorica, "Superoksid-dismutaza kao relevantan biomarker oksidacionog stresa kod slatkovodne školjke Unio pictorum na različitim lokalitetima-primer istraživanja reke Save" in The Danube in Serbia : the results of national program of the Second joint Danube survey 2007 (2010):225-240,
https://hdl.handle.net/21.15107/rcub_ibiss_6025 .

Age related differences in glutathione metabolism in freshwater snail, Viviparus acerosus from the Danube river

Despotović, Svetlana; Gavrilović, Branka; Radovanović, Tijana; Gavrić, Jelena; Borković-Mitić, Slavica; Pavlović, Slađan; Paunović, Momir; Pajović, Snežana; Saičić, Zorica

(Belgrade: University of Belgrade, Faculty of Biology, 2010)

TY  - CONF
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Paunović, Momir
AU  - Pajović, Snežana
AU  - Saičić, Zorica
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5949
AB  - Data are presented in this study on the tissue glutathione (GSH) content and on the activity on the main enzymes involved in GSH metabolism ( glutathione peroxidase - GSH-Px, glutathione reductase - GR and phase II biotransfor­ mation enzyme glutathione-S-transferase - GST) in the whole body of fresh­ water snail, Viviparus acerosus of two different age groups - young and adults from the Danube River. The obtained results show, that there was no differences in content of GSH in both investigating groups of snails. We observed that the activity of GR was significantly lower in adults in comparison to young groups. The activity of GST was markedly higher in adults in respect to the young snails. Moreover, GSH-Px activity did not change in different age groups, this possible indicating no variation in organic peroxide production and/or metabolism during ageing process. Ageing seems to affect the activity of GSH related enzymes and, in particular, the activities of GR and phase II biotransformation enzyme GST. Our work represents the first study of its kind and these data indicate that, in age­ ing freshwater snails, Viviparus acerosus from the Danube river the increased susceptibility to oxidative stress is partly related in alteration in glutathione me­ tabolism.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia
T1  - Age related differences in glutathione metabolism in freshwater snail, Viviparus acerosus from the Danube river
SP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5949
ER  - 
@conference{
author = "Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Gavrić, Jelena and Borković-Mitić, Slavica and Pavlović, Slađan and Paunović, Momir and Pajović, Snežana and Saičić, Zorica",
year = "2010",
abstract = "Data are presented in this study on the tissue glutathione (GSH) content and on the activity on the main enzymes involved in GSH metabolism ( glutathione peroxidase - GSH-Px, glutathione reductase - GR and phase II biotransfor­ mation enzyme glutathione-S-transferase - GST) in the whole body of fresh­ water snail, Viviparus acerosus of two different age groups - young and adults from the Danube River. The obtained results show, that there was no differences in content of GSH in both investigating groups of snails. We observed that the activity of GR was significantly lower in adults in comparison to young groups. The activity of GST was markedly higher in adults in respect to the young snails. Moreover, GSH-Px activity did not change in different age groups, this possible indicating no variation in organic peroxide production and/or metabolism during ageing process. Ageing seems to affect the activity of GSH related enzymes and, in particular, the activities of GR and phase II biotransformation enzyme GST. Our work represents the first study of its kind and these data indicate that, in age­ ing freshwater snails, Viviparus acerosus from the Danube river the increased susceptibility to oxidative stress is partly related in alteration in glutathione me­ tabolism.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia",
title = "Age related differences in glutathione metabolism in freshwater snail, Viviparus acerosus from the Danube river",
pages = "97",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5949"
}
Despotović, S., Gavrilović, B., Radovanović, T., Gavrić, J., Borković-Mitić, S., Pavlović, S., Paunović, M., Pajović, S.,& Saičić, Z.. (2010). Age related differences in glutathione metabolism in freshwater snail, Viviparus acerosus from the Danube river. in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 97.
https://hdl.handle.net/21.15107/rcub_ibiss_5949
Despotović S, Gavrilović B, Radovanović T, Gavrić J, Borković-Mitić S, Pavlović S, Paunović M, Pajović S, Saičić Z. Age related differences in glutathione metabolism in freshwater snail, Viviparus acerosus from the Danube river. in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia. 2010;:97.
https://hdl.handle.net/21.15107/rcub_ibiss_5949 .
Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Gavrić, Jelena, Borković-Mitić, Slavica, Pavlović, Slađan, Paunović, Momir, Pajović, Snežana, Saičić, Zorica, "Age related differences in glutathione metabolism in freshwater snail, Viviparus acerosus from the Danube river" in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia (2010):97,
https://hdl.handle.net/21.15107/rcub_ibiss_5949 .

Antioxidant defence responses to toxic cyanobacterial bloom in the liver of freshwater fish, Perca fluviatilis from the Gruža reservoir

Gavrilović, Branka; Despotović, Svetlana; Radovanović, Tijana; Gavrić, Jelena; Borković-Mitić, Slavica; Pavlović, Slađan; Ognjanović, Branka; Pajović, Snežana; Saičić, Zorica

(Belgrade: University of Belgrade, Faculty of Biology, 2010)

TY  - CONF
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Ognjanović, Branka
AU  - Pajović, Snežana
AU  - Saičić, Zorica
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5946
AB  - We investigated alterations of antioxidant defence system (ADS) in the liver of freshwater fish, European perch (Perea fluviatilis) as response to toxic Apha­ nizomenon flos-aquae bloom in the Gruza Reservoir. Activities of total, manga­ nese, copper zinc containing superoxide dismutase (Tot SOD, Mn SOD, CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), phase II biotransformation enzyme glutathione S-transferase (GST), con­ centrations of total glutathione (GSH) and sulphydryl (-SH) groups were mea­ sured before and during bloom period. Histopathological examinations were studied using electron microscopy. Activities of CuZn SOD, CAT and GSH-Px and concentration of -SH groups were significantly lower, while Mn SOD activ­ ity was significantly higher during bloom period. No differences were observed for Tot SOD, GR and GST activities, as well as for GSH concentration. Electron microscopy showed alterations in hepatocytes, which results in damage of cells. Our work represents the first study of its kind and suggest on influence of toxic Aphanizomenon flos-aquae bloom in the Gruza Reservoir on ADS and on histo­ pathological changes in the liver of Perea fiuviatilis indicating on necrotic and apopototic damages of hepatocytes.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia
T1  - Antioxidant defence responses to toxic cyanobacterial bloom in the liver of freshwater fish, Perca fluviatilis from the Gruža reservoir
SP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5946
ER  - 
@conference{
author = "Gavrilović, Branka and Despotović, Svetlana and Radovanović, Tijana and Gavrić, Jelena and Borković-Mitić, Slavica and Pavlović, Slađan and Ognjanović, Branka and Pajović, Snežana and Saičić, Zorica",
year = "2010",
abstract = "We investigated alterations of antioxidant defence system (ADS) in the liver of freshwater fish, European perch (Perea fluviatilis) as response to toxic Apha­ nizomenon flos-aquae bloom in the Gruza Reservoir. Activities of total, manga­ nese, copper zinc containing superoxide dismutase (Tot SOD, Mn SOD, CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), phase II biotransformation enzyme glutathione S-transferase (GST), con­ centrations of total glutathione (GSH) and sulphydryl (-SH) groups were mea­ sured before and during bloom period. Histopathological examinations were studied using electron microscopy. Activities of CuZn SOD, CAT and GSH-Px and concentration of -SH groups were significantly lower, while Mn SOD activ­ ity was significantly higher during bloom period. No differences were observed for Tot SOD, GR and GST activities, as well as for GSH concentration. Electron microscopy showed alterations in hepatocytes, which results in damage of cells. Our work represents the first study of its kind and suggest on influence of toxic Aphanizomenon flos-aquae bloom in the Gruza Reservoir on ADS and on histo­ pathological changes in the liver of Perea fiuviatilis indicating on necrotic and apopototic damages of hepatocytes.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia",
title = "Antioxidant defence responses to toxic cyanobacterial bloom in the liver of freshwater fish, Perca fluviatilis from the Gruža reservoir",
pages = "112",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5946"
}
Gavrilović, B., Despotović, S., Radovanović, T., Gavrić, J., Borković-Mitić, S., Pavlović, S., Ognjanović, B., Pajović, S.,& Saičić, Z.. (2010). Antioxidant defence responses to toxic cyanobacterial bloom in the liver of freshwater fish, Perca fluviatilis from the Gruža reservoir. in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 112.
https://hdl.handle.net/21.15107/rcub_ibiss_5946
Gavrilović B, Despotović S, Radovanović T, Gavrić J, Borković-Mitić S, Pavlović S, Ognjanović B, Pajović S, Saičić Z. Antioxidant defence responses to toxic cyanobacterial bloom in the liver of freshwater fish, Perca fluviatilis from the Gruža reservoir. in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia. 2010;:112.
https://hdl.handle.net/21.15107/rcub_ibiss_5946 .
Gavrilović, Branka, Despotović, Svetlana, Radovanović, Tijana, Gavrić, Jelena, Borković-Mitić, Slavica, Pavlović, Slađan, Ognjanović, Branka, Pajović, Snežana, Saičić, Zorica, "Antioxidant defence responses to toxic cyanobacterial bloom in the liver of freshwater fish, Perca fluviatilis from the Gruža reservoir" in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia (2010):112,
https://hdl.handle.net/21.15107/rcub_ibiss_5946 .

Antioxidant defence enzyme activities in some tissues of the freshwater bivalve Anodonta woodiana from the Sava river

Borković-Mitić, Slavica; Pavlović, Slađan; Radovanović, Tijana; Gavrilović, Branka; Despotović, Svetlana; Gavrić, Jelena; Paunović, Momir; Pajović, Snežana; Saičić, Zorica

(Belgrade: University of Belgrade, Faculty of Biology, 2010)

TY  - CONF
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Paunović, Momir
AU  - Pajović, Snežana
AU  - Saičić, Zorica
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5945
AB  - In the present study we have used the activity of aantioxidative defence enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the activity of glutathione S-transferase (GST), an enzyme of the phase II biotransformation process to evaluate their utility as biological toll for pollution monitoring in the Sava River. We also determined total protein content, as well as protein and SOD electrophoretic profiles in the digestive gland and the gills of the freshwater bivalve anodonta woodiana. Obtained results indicate a significant influence of tissue specifity of the all investigated enzyme activities. The activities of SOD, CAT, GSH-Px and phase II biotransformation enzyme GST were significantly higher in the digestive gland in respect to the gills, whereas GR activity exhibited higher levels in the gills. This was most likely a reflection of different metabolic activities and different responses to environmental conditions of the examined tissues. Our study also suggest, that the variation of SOD expression pattern in Anodonta woodiana could be used as a tool for the environmental monitoring. Our wo bivalve Anodonta woodiana friom the Sava Riverrk represents a first study of this kind and showed that the antioxidant defence enzymes can be considered as a potential biomarkers of the freshwater
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia
T1  - Antioxidant defence enzyme activities in some tissues of the freshwater bivalve Anodonta woodiana from the Sava river
SP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5945
ER  - 
@conference{
author = "Borković-Mitić, Slavica and Pavlović, Slađan and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Paunović, Momir and Pajović, Snežana and Saičić, Zorica",
year = "2010",
abstract = "In the present study we have used the activity of aantioxidative defence enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the activity of glutathione S-transferase (GST), an enzyme of the phase II biotransformation process to evaluate their utility as biological toll for pollution monitoring in the Sava River. We also determined total protein content, as well as protein and SOD electrophoretic profiles in the digestive gland and the gills of the freshwater bivalve anodonta woodiana. Obtained results indicate a significant influence of tissue specifity of the all investigated enzyme activities. The activities of SOD, CAT, GSH-Px and phase II biotransformation enzyme GST were significantly higher in the digestive gland in respect to the gills, whereas GR activity exhibited higher levels in the gills. This was most likely a reflection of different metabolic activities and different responses to environmental conditions of the examined tissues. Our study also suggest, that the variation of SOD expression pattern in Anodonta woodiana could be used as a tool for the environmental monitoring. Our wo bivalve Anodonta woodiana friom the Sava Riverrk represents a first study of this kind and showed that the antioxidant defence enzymes can be considered as a potential biomarkers of the freshwater",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia",
title = "Antioxidant defence enzyme activities in some tissues of the freshwater bivalve Anodonta woodiana from the Sava river",
pages = "95",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5945"
}
Borković-Mitić, S., Pavlović, S., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Paunović, M., Pajović, S.,& Saičić, Z.. (2010). Antioxidant defence enzyme activities in some tissues of the freshwater bivalve Anodonta woodiana from the Sava river. in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 95.
https://hdl.handle.net/21.15107/rcub_ibiss_5945
Borković-Mitić S, Pavlović S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Paunović M, Pajović S, Saičić Z. Antioxidant defence enzyme activities in some tissues of the freshwater bivalve Anodonta woodiana from the Sava river. in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia. 2010;:95.
https://hdl.handle.net/21.15107/rcub_ibiss_5945 .
Borković-Mitić, Slavica, Pavlović, Slađan, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Paunović, Momir, Pajović, Snežana, Saičić, Zorica, "Antioxidant defence enzyme activities in some tissues of the freshwater bivalve Anodonta woodiana from the Sava river" in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia (2010):95,
https://hdl.handle.net/21.15107/rcub_ibiss_5945 .

Biomarkers of oxidative stress in the liver of mediterranean fish, Trigla lucerna from the Adriatic sea

Pavlović, Slađan; Borković-Mitić, Slavica; Radovanović, Tijana; Gavrilović, Branka; Despotović, Svetlana; Gavrić, Jelena; Saičić, Zorica

(Belgrade: University of Belgrade, Faculty of Biology, 2010)

TY  - CONF
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Saičić, Zorica
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5944
AB  - We investigated the effects of season and locality on oxidative stress bio- markers: total, manganese and copper zinc containing superoxide dismutase (Tot SOD, Mn SOD, CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and phase II biotransformation enzyme glutathione S-transferase (GST) activity in the liver of Tub gurnard (Trigla lucerna) from the Adriatic sea. We also investigated the total protein concentration, as well as electrophoretic profiles of liver proteins. The specimens were collected in winter (February) and spring (May) at localities: Platamuni, Near Bar, Valdanos and Estuary of the River Bojana. The obtained results show significant differences in the activity of oxidative stress biomarkers in the liver of Tub gurnard (Trigla lucerna} between some localities in winter, as well as in spring season. We ob- tained significant seasonal differences for some of investigated parameters also. Our results are most pronounced for phase II biotransformation enzyme glutathi- one S-transferase (GST) activity suggesting that this enzyme is most valuable for this type of biomonitoring studies. Significant changes in total protein concentra- tion between some localities in winter and spring, but without seasonal effects were detected. Our work represents the first study of its kind and suggests that observed changes probably represents physiological adaptation to differences between environmental factors in the sea water, especially in the presence of various concentrations of natural or anthropogenic pollutants.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia
T1  - Biomarkers of oxidative stress in the liver of mediterranean fish, Trigla lucerna from the Adriatic sea
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5944
ER  - 
@conference{
author = "Pavlović, Slađan and Borković-Mitić, Slavica and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Saičić, Zorica",
year = "2010",
abstract = "We investigated the effects of season and locality on oxidative stress bio- markers: total, manganese and copper zinc containing superoxide dismutase (Tot SOD, Mn SOD, CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and phase II biotransformation enzyme glutathione S-transferase (GST) activity in the liver of Tub gurnard (Trigla lucerna) from the Adriatic sea. We also investigated the total protein concentration, as well as electrophoretic profiles of liver proteins. The specimens were collected in winter (February) and spring (May) at localities: Platamuni, Near Bar, Valdanos and Estuary of the River Bojana. The obtained results show significant differences in the activity of oxidative stress biomarkers in the liver of Tub gurnard (Trigla lucerna} between some localities in winter, as well as in spring season. We ob- tained significant seasonal differences for some of investigated parameters also. Our results are most pronounced for phase II biotransformation enzyme glutathi- one S-transferase (GST) activity suggesting that this enzyme is most valuable for this type of biomonitoring studies. Significant changes in total protein concentra- tion between some localities in winter and spring, but without seasonal effects were detected. Our work represents the first study of its kind and suggests that observed changes probably represents physiological adaptation to differences between environmental factors in the sea water, especially in the presence of various concentrations of natural or anthropogenic pollutants.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia",
title = "Biomarkers of oxidative stress in the liver of mediterranean fish, Trigla lucerna from the Adriatic sea",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5944"
}
Pavlović, S., Borković-Mitić, S., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J.,& Saičić, Z.. (2010). Biomarkers of oxidative stress in the liver of mediterranean fish, Trigla lucerna from the Adriatic sea. in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5944
Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Saičić Z. Biomarkers of oxidative stress in the liver of mediterranean fish, Trigla lucerna from the Adriatic sea. in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia. 2010;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5944 .
Pavlović, Slađan, Borković-Mitić, Slavica, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Saičić, Zorica, "Biomarkers of oxidative stress in the liver of mediterranean fish, Trigla lucerna from the Adriatic sea" in Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia (2010):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5944 .

Malakofauna Srpskog dela Dunava i istraživanih pritoka (Tisa, Sava i Velika Morava)

Tomović, Jelena; Vranković, Jelena; Tubić, Bojana; Borković-Mitić, Slavica; Pavlović, Slađan; Saičić, Zorica; Paunović, Momir

(Belgrade : Republic of Serbia, Ministry of agriculture, forestry and water management - Republic directorate of water, 2010)

TY  - CHAP
AU  - Tomović, Jelena
AU  - Vranković, Jelena
AU  - Tubić, Bojana
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
AU  - Paunović, Momir
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5929
AB  - The molluses were found to be one of the principal components of the aquatic ecosystems within the investigated stretches, thus we decided to present the characteristics of the community of this group in detail. A total of 31 molluse taxa were identified. The resull of Correspondence Analyses based on the distribution of molluses along the Danube River indicates difference in overall environmental conditions in the part of the Danube River upstream and downstream the site Stara Palanka-Ram (DS58), which is in concordance with the Danube typology.
AB  - The molluses were found to be one of the principal components of the aquatic ecosystems within the investigated stretches, thus we decided to present the characteristics of the community of this group in detail. A total of 31 molluse taxa were identified. The resull of Correspondence Analyses based on the distribution of molluses along the Danube River indicates difference in overall environmental conditions in the part of the Danube River upstream and downstream the site Stara Palanka-Ram (JDS58), which is in concordance with the Danube typology.
PB  - Belgrade : Republic of Serbia, Ministry of agriculture, forestry and water management - Republic directorate of water
T2  - The Danube in Serbia : the results of national program of the Second joint Danube survey 2007
T1  - Malakofauna Srpskog dela Dunava i istraživanih pritoka (Tisa, Sava i Velika Morava)
T1  - Malakofauna of the Serbian Stretch of the Danube River and Studied Tributaries (the Tisa, Sava and Velika Morava)
IS  - 3
VL  - 12
SP  - 207
EP  - 224
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5929
ER  - 
@inbook{
author = "Tomović, Jelena and Vranković, Jelena and Tubić, Bojana and Borković-Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica and Paunović, Momir",
year = "2010",
abstract = "The molluses were found to be one of the principal components of the aquatic ecosystems within the investigated stretches, thus we decided to present the characteristics of the community of this group in detail. A total of 31 molluse taxa were identified. The resull of Correspondence Analyses based on the distribution of molluses along the Danube River indicates difference in overall environmental conditions in the part of the Danube River upstream and downstream the site Stara Palanka-Ram (DS58), which is in concordance with the Danube typology., The molluses were found to be one of the principal components of the aquatic ecosystems within the investigated stretches, thus we decided to present the characteristics of the community of this group in detail. A total of 31 molluse taxa were identified. The resull of Correspondence Analyses based on the distribution of molluses along the Danube River indicates difference in overall environmental conditions in the part of the Danube River upstream and downstream the site Stara Palanka-Ram (JDS58), which is in concordance with the Danube typology.",
publisher = "Belgrade : Republic of Serbia, Ministry of agriculture, forestry and water management - Republic directorate of water",
journal = "The Danube in Serbia : the results of national program of the Second joint Danube survey 2007",
booktitle = "Malakofauna Srpskog dela Dunava i istraživanih pritoka (Tisa, Sava i Velika Morava), Malakofauna of the Serbian Stretch of the Danube River and Studied Tributaries (the Tisa, Sava and Velika Morava)",
number = "3",
volume = "12",
pages = "207-224",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5929"
}
Tomović, J., Vranković, J., Tubić, B., Borković-Mitić, S., Pavlović, S., Saičić, Z.,& Paunović, M.. (2010). Malakofauna Srpskog dela Dunava i istraživanih pritoka (Tisa, Sava i Velika Morava). in The Danube in Serbia : the results of national program of the Second joint Danube survey 2007
Belgrade : Republic of Serbia, Ministry of agriculture, forestry and water management - Republic directorate of water., 12(3), 207-224.
https://hdl.handle.net/21.15107/rcub_ibiss_5929
Tomović J, Vranković J, Tubić B, Borković-Mitić S, Pavlović S, Saičić Z, Paunović M. Malakofauna Srpskog dela Dunava i istraživanih pritoka (Tisa, Sava i Velika Morava). in The Danube in Serbia : the results of national program of the Second joint Danube survey 2007. 2010;12(3):207-224.
https://hdl.handle.net/21.15107/rcub_ibiss_5929 .
Tomović, Jelena, Vranković, Jelena, Tubić, Bojana, Borković-Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, Paunović, Momir, "Malakofauna Srpskog dela Dunava i istraživanih pritoka (Tisa, Sava i Velika Morava)" in The Danube in Serbia : the results of national program of the Second joint Danube survey 2007, 12, no. 3 (2010):207-224,
https://hdl.handle.net/21.15107/rcub_ibiss_5929 .

Aktivnosti glutation zavisnih enzima u jetri i mišiću mrene (Barbus barbus) i njenom crevnom parazitu (Pomphoryinchus laevis) iz Dunava

Radovanović, Tijana; Gavrilović, Branka; Despotović, Svetlana; Gavrić, Jelena; Borković-Mitić, Slavica; Pavlović, Slađan; Saičić, Zorica

(Belgrade : Serbian Society for Mitochondrial and Free-Radical Physiology, 2009)

TY  - CONF
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5955
AB  - Mrena (Barbus barbus) je slatkovodna, bentosna riba, široke geografske distribucije. Ona je krajnji domaćin adultne forme crevnog parazita Pomphoryinchus laevis (Acanthocephala).
U našem radu određivali smo aktivnosti glutation zavisnih enzima – glutation- peroksidaze (GSH-Px), glutation-reduktaze (GR) i enzima faze II biotransformacije glutation-S-transferaze (GST) kao i specifičnu koncentraciju proteina u jetri i mišiću mrene i njenom crevnom parazitu iz Dunava. Naša studija je izvedena u prigradskom području Beograda, između Višnjice i Grocke, u prolećnoj i letnjoj sezoni. Aktivnosti svih ispitivanih enzima merene su standardnim spektrofotometrijskim metodama i izražene su kao specifične u Jed/mg proteina.
Naši rezultati pokazuju značajno povećanu aktivnost GSH-Px u jetri i mišiću u prolećnoj sezoni u odnosu na letnju (p<0.05). Kod crevnog parazita aktivnost GSH-Px bila je povećana u letnjoj sezoni (p<0.05). Specifična GR aktivnost u jetri bila je smanjena u letnjoj sezoni, u poređenju sa prolećnom (p<0.05). Nije zabeležena značajna promena u GR aktivnosti između sezona u mišiću i parazitu. U jetri, mišiću i parazitu specifična aktivnost GST bila je značajno povećana u letnjoj sezoni (p<0.05). Specifična koncentracija proteina bila je u mišiću značajno povećana u letnjoj sezoni (p<0.05).
Naša istraživanja predstavljaju prvu procenu aktivnosti glutation  zavisnih enzima u jetri i mišiću mrene i njenom crevnom parazitu Pomphoryinchus laevis u srpskom delu Dunava. Možemo zaključiti da je sezonska fluktuacija ispitivanih glutation zavisnih enzima mrene i njenog crevnog parazita u korelaciji sa sezonskim temperaturnim varijacijama.
PB  - Belgrade : Serbian Society for Mitochondrial and Free-Radical Physiology
C3  - Knjiga sažetaka: Naučni simpozijum: Mitohondrije i slobodni radikali - nov izazov; 2009 Sep 21; Belgrade, Serbia
T1  - Aktivnosti glutation zavisnih enzima u jetri i mišiću mrene (Barbus barbus) i njenom crevnom parazitu (Pomphoryinchus laevis) iz Dunava
SP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5955
ER  - 
@conference{
author = "Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Borković-Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2009",
abstract = "Mrena (Barbus barbus) je slatkovodna, bentosna riba, široke geografske distribucije. Ona je krajnji domaćin adultne forme crevnog parazita Pomphoryinchus laevis (Acanthocephala).
U našem radu određivali smo aktivnosti glutation zavisnih enzima – glutation- peroksidaze (GSH-Px), glutation-reduktaze (GR) i enzima faze II biotransformacije glutation-S-transferaze (GST) kao i specifičnu koncentraciju proteina u jetri i mišiću mrene i njenom crevnom parazitu iz Dunava. Naša studija je izvedena u prigradskom području Beograda, između Višnjice i Grocke, u prolećnoj i letnjoj sezoni. Aktivnosti svih ispitivanih enzima merene su standardnim spektrofotometrijskim metodama i izražene su kao specifične u Jed/mg proteina.
Naši rezultati pokazuju značajno povećanu aktivnost GSH-Px u jetri i mišiću u prolećnoj sezoni u odnosu na letnju (p<0.05). Kod crevnog parazita aktivnost GSH-Px bila je povećana u letnjoj sezoni (p<0.05). Specifična GR aktivnost u jetri bila je smanjena u letnjoj sezoni, u poređenju sa prolećnom (p<0.05). Nije zabeležena značajna promena u GR aktivnosti između sezona u mišiću i parazitu. U jetri, mišiću i parazitu specifična aktivnost GST bila je značajno povećana u letnjoj sezoni (p<0.05). Specifična koncentracija proteina bila je u mišiću značajno povećana u letnjoj sezoni (p<0.05).
Naša istraživanja predstavljaju prvu procenu aktivnosti glutation  zavisnih enzima u jetri i mišiću mrene i njenom crevnom parazitu Pomphoryinchus laevis u srpskom delu Dunava. Možemo zaključiti da je sezonska fluktuacija ispitivanih glutation zavisnih enzima mrene i njenog crevnog parazita u korelaciji sa sezonskim temperaturnim varijacijama.",
publisher = "Belgrade : Serbian Society for Mitochondrial and Free-Radical Physiology",
journal = "Knjiga sažetaka: Naučni simpozijum: Mitohondrije i slobodni radikali - nov izazov; 2009 Sep 21; Belgrade, Serbia",
title = "Aktivnosti glutation zavisnih enzima u jetri i mišiću mrene (Barbus barbus) i njenom crevnom parazitu (Pomphoryinchus laevis) iz Dunava",
pages = "92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5955"
}
Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Borković-Mitić, S., Pavlović, S.,& Saičić, Z.. (2009). Aktivnosti glutation zavisnih enzima u jetri i mišiću mrene (Barbus barbus) i njenom crevnom parazitu (Pomphoryinchus laevis) iz Dunava. in Knjiga sažetaka: Naučni simpozijum: Mitohondrije i slobodni radikali - nov izazov; 2009 Sep 21; Belgrade, Serbia
Belgrade : Serbian Society for Mitochondrial and Free-Radical Physiology., 92.
https://hdl.handle.net/21.15107/rcub_ibiss_5955
Radovanović T, Gavrilović B, Despotović S, Gavrić J, Borković-Mitić S, Pavlović S, Saičić Z. Aktivnosti glutation zavisnih enzima u jetri i mišiću mrene (Barbus barbus) i njenom crevnom parazitu (Pomphoryinchus laevis) iz Dunava. in Knjiga sažetaka: Naučni simpozijum: Mitohondrije i slobodni radikali - nov izazov; 2009 Sep 21; Belgrade, Serbia. 2009;:92.
https://hdl.handle.net/21.15107/rcub_ibiss_5955 .
Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Borković-Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Aktivnosti glutation zavisnih enzima u jetri i mišiću mrene (Barbus barbus) i njenom crevnom parazitu (Pomphoryinchus laevis) iz Dunava" in Knjiga sažetaka: Naučni simpozijum: Mitohondrije i slobodni radikali - nov izazov; 2009 Sep 21; Belgrade, Serbia (2009):92,
https://hdl.handle.net/21.15107/rcub_ibiss_5955 .

Biomarkeri oksidacionog stresa u jetri oslića (Merluccius merluccius l.) iz Jadranskog mora

Pavlović, Slađan; Borković-Mitić, Slavica; Radovanović, Tijana; Gavrilović, Branka; Despotović, Svetlana; Gavrić, Jelena; Saičić, Zorica

(Belgrade : Serbian Society for Mitochondrial and Free-Radical Physiology, 2009)

TY  - CONF
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Saičić, Zorica
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5954
AB  - Morski ekosistemi predstavljaju glavno mesto izlivanja prirodnih i antropogenih zagađivača. Veliki broj biomarkera se koristi kao efikasan rano upozoravajući mehanizam u proceni stanja životne sredine (ERA). Fiziološki status  morskih organizama može biti promenjen kada se izlože zagađivačima. Jedna od posledica može biti smanjena sposobnost organizama da tolerišu prirodne fluktuacije spoljašnjih faktora. Cilj ove studije bio je istraživanje efekta lokaliteta na biomarkere oksidacionog stresa: ukupnu, mangan sadržavajuću i bakar cink sadržavajuću superoksid-dismutazu (Tot SOD, Mn SOD, CuZn SOD), katalazu (CAT), glutation-peroksidazu (GSH-Px), glutation-reduktazu (GR) i enzim faze II biotransformacije glutation-S-transferazu (GST), kao i koncentraciju ukupnih proteina u jetri oslića (Merluccius merluccius L.) iz Jadranskog mora.
Uzorci su sakupljani u zimskom periodu (Februar) na lokalitetima: Platamuni (PL), ispred Bara (NB), Valdanos (VAL) i ušće Bojane (ERB). Aktivnost svih  ispitivanih enzima merena je korišćenjem standardnih spektrofotometrijskih metoda, a sve enzimske aktivnosti su izražene kao specifične u Jed/mg proteina.
Naši rezultati pokazuju da između ispitivanih lokaliteta nisu zabeležene  značajne razlike u koncentraciji ukupnih proteina. Međutim, dobili smo povećanu aktivnost CAT na lokalitetu PL u odnosu na NB, kao i u odnosu na VAL (p<0.05). U  isto vreme, aktivnost GSH-Px bila je značajno veća na lokalitetu NB u poređenju sa PL, VAL i ERB (p<0.05). Aktivnost GR bila je značajno smanjena na lokalitetima PL i VAL u odnosu na NB i ERB (p<0.05). Aktivnost enzima faze II biotransformacije GST bila je značajno smanjena na lokalitetu VAL u poređenju sa svim ostalim ispitivanim lokalitetima (p<0.05).
Iz prikazanih rezultata, može se zaključiti da u jetri oslića (Merluccius merluccius L.) iz Jadranskog mora postoje razlike u aktivnosti biomarkera oksidacionog stresa između različitih lokaliteta što verovatno predstavlja fiziološku adaptaciju na razlike u spoljašnjim faktorima u morskoj vodi, naročito na prisustvo različitih koncentracija prirodnih ili antropogenih zagađivača.
PB  - Belgrade : Serbian Society for Mitochondrial and Free-Radical Physiology
C3  - Knjiga sažetaka: Naučni simpozijum: Mitohondrije i slobodni radikali - nov izazov; 2009 Sep 21; Belgrade, Serbia
T1  - Biomarkeri oksidacionog stresa u jetri oslića (Merluccius merluccius l.) iz Jadranskog mora
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5954
ER  - 
@conference{
author = "Pavlović, Slađan and Borković-Mitić, Slavica and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Saičić, Zorica",
year = "2009",
abstract = "Morski ekosistemi predstavljaju glavno mesto izlivanja prirodnih i antropogenih zagađivača. Veliki broj biomarkera se koristi kao efikasan rano upozoravajući mehanizam u proceni stanja životne sredine (ERA). Fiziološki status  morskih organizama može biti promenjen kada se izlože zagađivačima. Jedna od posledica može biti smanjena sposobnost organizama da tolerišu prirodne fluktuacije spoljašnjih faktora. Cilj ove studije bio je istraživanje efekta lokaliteta na biomarkere oksidacionog stresa: ukupnu, mangan sadržavajuću i bakar cink sadržavajuću superoksid-dismutazu (Tot SOD, Mn SOD, CuZn SOD), katalazu (CAT), glutation-peroksidazu (GSH-Px), glutation-reduktazu (GR) i enzim faze II biotransformacije glutation-S-transferazu (GST), kao i koncentraciju ukupnih proteina u jetri oslića (Merluccius merluccius L.) iz Jadranskog mora.
Uzorci su sakupljani u zimskom periodu (Februar) na lokalitetima: Platamuni (PL), ispred Bara (NB), Valdanos (VAL) i ušće Bojane (ERB). Aktivnost svih  ispitivanih enzima merena je korišćenjem standardnih spektrofotometrijskih metoda, a sve enzimske aktivnosti su izražene kao specifične u Jed/mg proteina.
Naši rezultati pokazuju da između ispitivanih lokaliteta nisu zabeležene  značajne razlike u koncentraciji ukupnih proteina. Međutim, dobili smo povećanu aktivnost CAT na lokalitetu PL u odnosu na NB, kao i u odnosu na VAL (p<0.05). U  isto vreme, aktivnost GSH-Px bila je značajno veća na lokalitetu NB u poređenju sa PL, VAL i ERB (p<0.05). Aktivnost GR bila je značajno smanjena na lokalitetima PL i VAL u odnosu na NB i ERB (p<0.05). Aktivnost enzima faze II biotransformacije GST bila je značajno smanjena na lokalitetu VAL u poređenju sa svim ostalim ispitivanim lokalitetima (p<0.05).
Iz prikazanih rezultata, može se zaključiti da u jetri oslića (Merluccius merluccius L.) iz Jadranskog mora postoje razlike u aktivnosti biomarkera oksidacionog stresa između različitih lokaliteta što verovatno predstavlja fiziološku adaptaciju na razlike u spoljašnjim faktorima u morskoj vodi, naročito na prisustvo različitih koncentracija prirodnih ili antropogenih zagađivača.",
publisher = "Belgrade : Serbian Society for Mitochondrial and Free-Radical Physiology",
journal = "Knjiga sažetaka: Naučni simpozijum: Mitohondrije i slobodni radikali - nov izazov; 2009 Sep 21; Belgrade, Serbia",
title = "Biomarkeri oksidacionog stresa u jetri oslića (Merluccius merluccius l.) iz Jadranskog mora",
pages = "32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5954"
}
Pavlović, S., Borković-Mitić, S., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J.,& Saičić, Z.. (2009). Biomarkeri oksidacionog stresa u jetri oslića (Merluccius merluccius l.) iz Jadranskog mora. in Knjiga sažetaka: Naučni simpozijum: Mitohondrije i slobodni radikali - nov izazov; 2009 Sep 21; Belgrade, Serbia
Belgrade : Serbian Society for Mitochondrial and Free-Radical Physiology., 32.
https://hdl.handle.net/21.15107/rcub_ibiss_5954
Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Saičić Z. Biomarkeri oksidacionog stresa u jetri oslića (Merluccius merluccius l.) iz Jadranskog mora. in Knjiga sažetaka: Naučni simpozijum: Mitohondrije i slobodni radikali - nov izazov; 2009 Sep 21; Belgrade, Serbia. 2009;:32.
https://hdl.handle.net/21.15107/rcub_ibiss_5954 .
Pavlović, Slađan, Borković-Mitić, Slavica, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Saičić, Zorica, "Biomarkeri oksidacionog stresa u jetri oslića (Merluccius merluccius l.) iz Jadranskog mora" in Knjiga sažetaka: Naučni simpozijum: Mitohondrije i slobodni radikali - nov izazov; 2009 Sep 21; Belgrade, Serbia (2009):32,
https://hdl.handle.net/21.15107/rcub_ibiss_5954 .

Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea

Ognjanović, Branka; Marković, Snežana; Đorđević, Nataša; Pavlović, Slađan; Štajn, Andraš Š.; Žikić, Radoslav V.; Saičić, Zorica

(Novi Sad: Faculty of Sciences, Department of Biology and Ecology, 2007)

TY  - CONF
AU  - Ognjanović, Branka
AU  - Marković, Snežana
AU  - Đorđević, Nataša
AU  - Pavlović, Slađan
AU  - Štajn, Andraš Š.
AU  - Žikić, Radoslav V.
AU  - Saičić, Zorica
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5999
AB  - The environment is continuously loaded with foreign organic chemicals (xenobiotics) released by urban communities and industries. Many classes of environmental contaminants (or their metabolites) are know to enhance the intracellular formation of reactive oxygen species (ROS) causing oxidative damage to biological systems. Fish are the most important organisms used in biomonitoring of aquatic ecosystems. Parameters of oxidative stress in fish tissues represent significant biomarkers in the assessment of the status of environment.
Specimens two marine fishes of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) were collected May 2005 from the locality in front of sea-port Bar (South Adriatic Sea). Parameters of oxidative stress: activity of antioxidative enzymes (superoxide dismutase­ SOD and catalase-CAT) and concentration of lipid peroxidation-LP and glutathione-GSH were determined in the liver of hake and red mullet.
Obtained results showed that activities of SOD and CAT were significantly lower in the liver of hake than in red mullet. Higher concentrations of LP and GSH in liver of hake were recorded, due to changes ofphysical­ chemical parameters of environment. The intensity of oxidative stress in liver of hake was higher in comparison to red mullet. This increased ROS production and subsequent oxidati e damage has been associated with pollutant mediated mechanism of toxicity in liver of investigated fishes.
According to the analyses of our results the investigated parameters of oxidative stress (concentration of LP and GSH, as well as activity of antioxidative enzymes SOD and CAT) in liver of hake and red mullet demonstrate     significant     and     reliable     biomarkers     m  monitoring,environmental  pollution.
PB  - Novi Sad: Faculty of Sciences, Department of Biology and Ecology
C3  - Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
T1  - Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5999
ER  - 
@conference{
author = "Ognjanović, Branka and Marković, Snežana and Đorđević, Nataša and Pavlović, Slađan and Štajn, Andraš Š. and Žikić, Radoslav V. and Saičić, Zorica",
year = "2007",
abstract = "The environment is continuously loaded with foreign organic chemicals (xenobiotics) released by urban communities and industries. Many classes of environmental contaminants (or their metabolites) are know to enhance the intracellular formation of reactive oxygen species (ROS) causing oxidative damage to biological systems. Fish are the most important organisms used in biomonitoring of aquatic ecosystems. Parameters of oxidative stress in fish tissues represent significant biomarkers in the assessment of the status of environment.
Specimens two marine fishes of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) were collected May 2005 from the locality in front of sea-port Bar (South Adriatic Sea). Parameters of oxidative stress: activity of antioxidative enzymes (superoxide dismutase­ SOD and catalase-CAT) and concentration of lipid peroxidation-LP and glutathione-GSH were determined in the liver of hake and red mullet.
Obtained results showed that activities of SOD and CAT were significantly lower in the liver of hake than in red mullet. Higher concentrations of LP and GSH in liver of hake were recorded, due to changes ofphysical­ chemical parameters of environment. The intensity of oxidative stress in liver of hake was higher in comparison to red mullet. This increased ROS production and subsequent oxidati e damage has been associated with pollutant mediated mechanism of toxicity in liver of investigated fishes.
According to the analyses of our results the investigated parameters of oxidative stress (concentration of LP and GSH, as well as activity of antioxidative enzymes SOD and CAT) in liver of hake and red mullet demonstrate     significant     and     reliable     biomarkers     m  monitoring,environmental  pollution.",
publisher = "Novi Sad: Faculty of Sciences, Department of Biology and Ecology",
journal = "Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia",
title = "Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5999"
}
Ognjanović, B., Marković, S., Đorđević, N., Pavlović, S., Štajn, A. Š., Žikić, R. V.,& Saičić, Z.. (2007). Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
Novi Sad: Faculty of Sciences, Department of Biology and Ecology., 69.
https://hdl.handle.net/21.15107/rcub_ibiss_5999
Ognjanović B, Marković S, Đorđević N, Pavlović S, Štajn AŠ, Žikić RV, Saičić Z. Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia. 2007;:69.
https://hdl.handle.net/21.15107/rcub_ibiss_5999 .
Ognjanović, Branka, Marković, Snežana, Đorđević, Nataša, Pavlović, Slađan, Štajn, Andraš Š., Žikić, Radoslav V., Saičić, Zorica, "Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea" in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia (2007):69,
https://hdl.handle.net/21.15107/rcub_ibiss_5999 .

Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea

Đorđević, Nataša; Ognjanović, Branka; Marković, Snežana; Pavlović, Slađan; Žikić, Radoslav V.; Štajn, Andraš Š.; Saičić, Zorica

(Novi Sad: Faculty of Sciences, Department of Biology and Ecology, 2007)

TY  - CONF
AU  - Đorđević, Nataša
AU  - Ognjanović, Branka
AU  - Marković, Snežana
AU  - Pavlović, Slađan
AU  - Žikić, Radoslav V.
AU  - Štajn, Andraš Š.
AU  - Saičić, Zorica
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5998
AB  - Recent investigations in biomonitoring include the monitoring of biochemical and physiological parameters to evaluate the condition of environment. Parameters of oxidative stress are significant biomarkers of environmental pollution of the sea. Specimens of marine fish - hake (Merluccius merluccius) was collected from the localities of Platamuni and Valdanos (Sauth Adriatic) in winter   and   spring   during   2003   year.   Concentrations   of antioxidant compounds such as Vitamin E (Vit E) and Vitamin C (Vit C) as well as, lipid peroxidation (LP) were determined in the liver and white muscle of the hake. Physical chemical parameters of water of investigated  localities  were also determined: salinity, temperature, oxygen saturation and concentrations of nitrites, nitrates and detergent in both seasons.
The obtained results showed higher concentrations of LP, Vit E and Vit C in liver of hake in comparison to white muscle. Concentrations of LP and Vit C were increased, while the concentrations of Vit E were decreased in winter period in comparison to spring. Concentrations of LP were increased while the concentrations of Vit E were decreased in both investigated tissues of hake from the waters of locality Valdanos in respect to Platamuni. These changes of parameters of oxidative stress are, in part, the consequence of changes of temperature as well as, concentrations of nitrites, nitrates and detergents in water of investigated localities and in particular season.
In conclusion, the concentrations of parameter of oxidative stress in investigated tissues of hake represent a good marker in the biomonitoring of environmental pollution. The intensity of oxidative stress was increased in the liver of hake in comparison to white muscle, in winter period ill respect to spring and in waters of Valdanos in comparison to Platamuni
PB  - Novi Sad: Faculty of Sciences, Department of Biology and Ecology
C3  - Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
T1  - Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea
SP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5998
ER  - 
@conference{
author = "Đorđević, Nataša and Ognjanović, Branka and Marković, Snežana and Pavlović, Slađan and Žikić, Radoslav V. and Štajn, Andraš Š. and Saičić, Zorica",
year = "2007",
abstract = "Recent investigations in biomonitoring include the monitoring of biochemical and physiological parameters to evaluate the condition of environment. Parameters of oxidative stress are significant biomarkers of environmental pollution of the sea. Specimens of marine fish - hake (Merluccius merluccius) was collected from the localities of Platamuni and Valdanos (Sauth Adriatic) in winter   and   spring   during   2003   year.   Concentrations   of antioxidant compounds such as Vitamin E (Vit E) and Vitamin C (Vit C) as well as, lipid peroxidation (LP) were determined in the liver and white muscle of the hake. Physical chemical parameters of water of investigated  localities  were also determined: salinity, temperature, oxygen saturation and concentrations of nitrites, nitrates and detergent in both seasons.
The obtained results showed higher concentrations of LP, Vit E and Vit C in liver of hake in comparison to white muscle. Concentrations of LP and Vit C were increased, while the concentrations of Vit E were decreased in winter period in comparison to spring. Concentrations of LP were increased while the concentrations of Vit E were decreased in both investigated tissues of hake from the waters of locality Valdanos in respect to Platamuni. These changes of parameters of oxidative stress are, in part, the consequence of changes of temperature as well as, concentrations of nitrites, nitrates and detergents in water of investigated localities and in particular season.
In conclusion, the concentrations of parameter of oxidative stress in investigated tissues of hake represent a good marker in the biomonitoring of environmental pollution. The intensity of oxidative stress was increased in the liver of hake in comparison to white muscle, in winter period ill respect to spring and in waters of Valdanos in comparison to Platamuni",
publisher = "Novi Sad: Faculty of Sciences, Department of Biology and Ecology",
journal = "Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia",
title = "Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea",
pages = "55",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5998"
}
Đorđević, N., Ognjanović, B., Marković, S., Pavlović, S., Žikić, R. V., Štajn, A. Š.,& Saičić, Z.. (2007). Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
Novi Sad: Faculty of Sciences, Department of Biology and Ecology., 55.
https://hdl.handle.net/21.15107/rcub_ibiss_5998
Đorđević N, Ognjanović B, Marković S, Pavlović S, Žikić RV, Štajn AŠ, Saičić Z. Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia. 2007;:55.
https://hdl.handle.net/21.15107/rcub_ibiss_5998 .
Đorđević, Nataša, Ognjanović, Branka, Marković, Snežana, Pavlović, Slađan, Žikić, Radoslav V., Štajn, Andraš Š., Saičić, Zorica, "Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea" in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia (2007):55,
https://hdl.handle.net/21.15107/rcub_ibiss_5998 .

Antioxidant effect of coenzyme Q10 and vitamin E in Cadmium induced alterations of antioxidant defense system in the rat heart.

Ognjanović, Branka; Marković, Snežana; Pavlović, Slađan; Žikić, Radoslav V.; Štajn, Andraš Š.; Saičić, Zorica

(Belgrade: Serbian Physiology Society, 2007)

TY  - CONF
AU  - Ognjanović, Branka
AU  - Marković, Snežana
AU  - Pavlović, Slađan
AU  - Žikić, Radoslav V.
AU  - Štajn, Andraš Š.
AU  - Saičić, Zorica
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5971
AB  - Cadmium (Cd) is  an  ubiquitous,  nonessential  element  that  has  recentl y  raised concerns due to its accumulation in the environment. The toxicity of Cd as an industrial  pollutant  a  food  contaminant and  as  one  of  the  major  components   in  cigarette smoke has been well established. A number of nutrient s have been shown to interact with Cd and alter its cellular effects. Several protective agents, including conzyme Q10 (CoQ10) and vitamin E (Vit E) play an important role in detoxification of  endogenous  and  exogenous  compounds.  Our  study  investigated  the possible protective  effects  of  coenzyme Q10  (CoQ 10)  and  vitamin   E  (Vit  E)  alone  or  in cornbrnation against cadmium (Cd) induced alterations of antioxidant defense system  in the rat heart. Male Wistar rats were injected with a single dose of CdCl2 (0.4 mg Cd/kg  i.p.),  CoQ10 (20  mg CoQ 1 0/kg  i. m.) and  Vit E (20  lU  Vit  E/kg, i. m.), a lone or in combination. Acute  intoxication  of  rats with Cd  were followed  by significantly  increased activity of antioxidant defense enzymes (CuZn SOD, GSH-Px, GST and GR), while the activity of Mn SOD  was decreased in the heart. The treatment with Cd significantly decreased Vit C and Vit E concentrations . Treatment with CoQ10+Vit E reversed  Cd-induced  alterations  in Vit C and  Vit  E concentrations. Our study suggests that the prooxidative effect of Cd is respon si ble for  alterations in prooxidant-ant ioxidant balance in the heart. The obtained results support the assumption   that   CoQ10  and Vit E functions   cooperatively with endogenous antioxidants and diminished some toxic effects of Cd in rat hearts .
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract book: A Scientific Conference with International Participation: Nutrition, Treatment and Cardiovascular Risk Management; 2007 May 24-27; Novi Sad, Serbia
T1  - Antioxidant effect of coenzyme Q10 and vitamin E in Cadmium induced alterations of antioxidant defense system in the rat heart.
SP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5971
ER  - 
@conference{
author = "Ognjanović, Branka and Marković, Snežana and Pavlović, Slađan and Žikić, Radoslav V. and Štajn, Andraš Š. and Saičić, Zorica",
year = "2007",
abstract = "Cadmium (Cd) is  an  ubiquitous,  nonessential  element  that  has  recentl y  raised concerns due to its accumulation in the environment. The toxicity of Cd as an industrial  pollutant  a  food  contaminant and  as  one  of  the  major  components   in  cigarette smoke has been well established. A number of nutrient s have been shown to interact with Cd and alter its cellular effects. Several protective agents, including conzyme Q10 (CoQ10) and vitamin E (Vit E) play an important role in detoxification of  endogenous  and  exogenous  compounds.  Our  study  investigated  the possible protective  effects  of  coenzyme Q10  (CoQ 10)  and  vitamin   E  (Vit  E)  alone  or  in cornbrnation against cadmium (Cd) induced alterations of antioxidant defense system  in the rat heart. Male Wistar rats were injected with a single dose of CdCl2 (0.4 mg Cd/kg  i.p.),  CoQ10 (20  mg CoQ 1 0/kg  i. m.) and  Vit E (20  lU  Vit  E/kg, i. m.), a lone or in combination. Acute  intoxication  of  rats with Cd  were followed  by significantly  increased activity of antioxidant defense enzymes (CuZn SOD, GSH-Px, GST and GR), while the activity of Mn SOD  was decreased in the heart. The treatment with Cd significantly decreased Vit C and Vit E concentrations . Treatment with CoQ10+Vit E reversed  Cd-induced  alterations  in Vit C and  Vit  E concentrations. Our study suggests that the prooxidative effect of Cd is respon si ble for  alterations in prooxidant-ant ioxidant balance in the heart. The obtained results support the assumption   that   CoQ10  and Vit E functions   cooperatively with endogenous antioxidants and diminished some toxic effects of Cd in rat hearts .",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract book: A Scientific Conference with International Participation: Nutrition, Treatment and Cardiovascular Risk Management; 2007 May 24-27; Novi Sad, Serbia",
title = "Antioxidant effect of coenzyme Q10 and vitamin E in Cadmium induced alterations of antioxidant defense system in the rat heart.",
pages = "112",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5971"
}
Ognjanović, B., Marković, S., Pavlović, S., Žikić, R. V., Štajn, A. Š.,& Saičić, Z.. (2007). Antioxidant effect of coenzyme Q10 and vitamin E in Cadmium induced alterations of antioxidant defense system in the rat heart.. in Abstract book: A Scientific Conference with International Participation: Nutrition, Treatment and Cardiovascular Risk Management; 2007 May 24-27; Novi Sad, Serbia
Belgrade: Serbian Physiology Society., 112.
https://hdl.handle.net/21.15107/rcub_ibiss_5971
Ognjanović B, Marković S, Pavlović S, Žikić RV, Štajn AŠ, Saičić Z. Antioxidant effect of coenzyme Q10 and vitamin E in Cadmium induced alterations of antioxidant defense system in the rat heart.. in Abstract book: A Scientific Conference with International Participation: Nutrition, Treatment and Cardiovascular Risk Management; 2007 May 24-27; Novi Sad, Serbia. 2007;:112.
https://hdl.handle.net/21.15107/rcub_ibiss_5971 .
Ognjanović, Branka, Marković, Snežana, Pavlović, Slađan, Žikić, Radoslav V., Štajn, Andraš Š., Saičić, Zorica, "Antioxidant effect of coenzyme Q10 and vitamin E in Cadmium induced alterations of antioxidant defense system in the rat heart." in Abstract book: A Scientific Conference with International Participation: Nutrition, Treatment and Cardiovascular Risk Management; 2007 May 24-27; Novi Sad, Serbia (2007):112,
https://hdl.handle.net/21.15107/rcub_ibiss_5971 .

Effect of cadmium on antioxidant defense system: changes after co-treatment with coenzyme Q10 in some tissues of rats.

Pavlović, Slađan; Ognjanović, Branka; Štajn, Andraš Š.; Žikić, Radoslav V.; Saičić, Zorica

(Belgrade: Serbian Physiology Society, 2006)

TY  - CONF
AU  - Pavlović, Slađan
AU  - Ognjanović, Branka
AU  - Štajn, Andraš Š.
AU  - Žikić, Radoslav V.
AU  - Saičić, Zorica
PY  - 2006
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5969
AB  - Cadmium (Cd) is a commonly occurring environmental contaminant which after being intake into the organism accumulates mostly in the liver and kidneys. Recent studies on mammals have shown that Cd stimulates formation of superoxide anion radicals, hydrogen peroxide and hydroxyl radicals. As a consequence, enhanced lipid peroxidation, DNA damage, altered calcium and sulfhydryl homeostasis, as well as marked disturbances of antioxidant defense system occur.
Coenzyme Q10 (CoQ10) in its reduced form (CoQ10H2) is a powerful antioxidant. As an antioxidant it is exploited either directly upon superoxide or indirectly on lipid radicals. CoQ10 may prevent both the initiation and propagation of lipid peroxidation, protects DNA from oxidation causing by lipid peroxidation and protects organism from oxidative stress induced by various toxic agents. 
	The activities of Total SOD, Mn SOD, CuZn SOD, CAT, GSH-Px, GR and phase II biotransformation enzyme-GST, as well as Vit C and Vit E concentrations were analyzed in the liver, heart and kidneys of male, two months old Wistar albino rats treated with Cd (200 mg CdCl2 x H2O, in drinking water during 30 days), CoQ10 (20 mg/kg CoQ10 dissolved in olive oil, i.m., every fifth day), concomitantly treated with Cd and CoQ10 (200 mg CdCl2 x H2O, in drinking water during 30 days + 20 mg/kg CoQ10 dissolved in olive oil, i.m., every fifth day) and in control animals (C). 
	 This study suggested that Cd influences antioxidative defense enzyme activities in the liver, heart and kidneys of rats in various ways, especially depending on its metabolic activity. CoQ10 only partially (and tissue-specific) reversed Cd-induced changes on these enzymes. At the same time, CoQ10 exerts very strong beneficial effects on Vit C and Vit E by elevating its concentrations and thus induced an increase in antioxidative defense potential in investigated tissues of rats treated with Cd.
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract book: The 2nd Multidisciplinary Scientific Meeting with International participation: Oxidative Stress and the Mechanisms of Protection; 2006 Nov 2-3; Kragujevac, Serbia
T1  - Effect of cadmium on antioxidant defense system: changes after co-treatment with coenzyme Q10 in some tissues of rats.
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5969
ER  - 
@conference{
author = "Pavlović, Slađan and Ognjanović, Branka and Štajn, Andraš Š. and Žikić, Radoslav V. and Saičić, Zorica",
year = "2006",
abstract = "Cadmium (Cd) is a commonly occurring environmental contaminant which after being intake into the organism accumulates mostly in the liver and kidneys. Recent studies on mammals have shown that Cd stimulates formation of superoxide anion radicals, hydrogen peroxide and hydroxyl radicals. As a consequence, enhanced lipid peroxidation, DNA damage, altered calcium and sulfhydryl homeostasis, as well as marked disturbances of antioxidant defense system occur.
Coenzyme Q10 (CoQ10) in its reduced form (CoQ10H2) is a powerful antioxidant. As an antioxidant it is exploited either directly upon superoxide or indirectly on lipid radicals. CoQ10 may prevent both the initiation and propagation of lipid peroxidation, protects DNA from oxidation causing by lipid peroxidation and protects organism from oxidative stress induced by various toxic agents. 
	The activities of Total SOD, Mn SOD, CuZn SOD, CAT, GSH-Px, GR and phase II biotransformation enzyme-GST, as well as Vit C and Vit E concentrations were analyzed in the liver, heart and kidneys of male, two months old Wistar albino rats treated with Cd (200 mg CdCl2 x H2O, in drinking water during 30 days), CoQ10 (20 mg/kg CoQ10 dissolved in olive oil, i.m., every fifth day), concomitantly treated with Cd and CoQ10 (200 mg CdCl2 x H2O, in drinking water during 30 days + 20 mg/kg CoQ10 dissolved in olive oil, i.m., every fifth day) and in control animals (C). 
	 This study suggested that Cd influences antioxidative defense enzyme activities in the liver, heart and kidneys of rats in various ways, especially depending on its metabolic activity. CoQ10 only partially (and tissue-specific) reversed Cd-induced changes on these enzymes. At the same time, CoQ10 exerts very strong beneficial effects on Vit C and Vit E by elevating its concentrations and thus induced an increase in antioxidative defense potential in investigated tissues of rats treated with Cd.",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract book: The 2nd Multidisciplinary Scientific Meeting with International participation: Oxidative Stress and the Mechanisms of Protection; 2006 Nov 2-3; Kragujevac, Serbia",
title = "Effect of cadmium on antioxidant defense system: changes after co-treatment with coenzyme Q10 in some tissues of rats.",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5969"
}
Pavlović, S., Ognjanović, B., Štajn, A. Š., Žikić, R. V.,& Saičić, Z.. (2006). Effect of cadmium on antioxidant defense system: changes after co-treatment with coenzyme Q10 in some tissues of rats.. in Abstract book: The 2nd Multidisciplinary Scientific Meeting with International participation: Oxidative Stress and the Mechanisms of Protection; 2006 Nov 2-3; Kragujevac, Serbia
Belgrade: Serbian Physiology Society., 33.
https://hdl.handle.net/21.15107/rcub_ibiss_5969
Pavlović S, Ognjanović B, Štajn AŠ, Žikić RV, Saičić Z. Effect of cadmium on antioxidant defense system: changes after co-treatment with coenzyme Q10 in some tissues of rats.. in Abstract book: The 2nd Multidisciplinary Scientific Meeting with International participation: Oxidative Stress and the Mechanisms of Protection; 2006 Nov 2-3; Kragujevac, Serbia. 2006;:33.
https://hdl.handle.net/21.15107/rcub_ibiss_5969 .
Pavlović, Slađan, Ognjanović, Branka, Štajn, Andraš Š., Žikić, Radoslav V., Saičić, Zorica, "Effect of cadmium on antioxidant defense system: changes after co-treatment with coenzyme Q10 in some tissues of rats." in Abstract book: The 2nd Multidisciplinary Scientific Meeting with International participation: Oxidative Stress and the Mechanisms of Protection; 2006 Nov 2-3; Kragujevac, Serbia (2006):33,
https://hdl.handle.net/21.15107/rcub_ibiss_5969 .

Lipid peroxidation and glutathione content in tissues of hake (Merluccius merluccius L.) and sea bream (Pagellus erythrinus L.) from the Adriatic sea

Ognjanović, Branka; Filipović, Jasmina; Nikolić, Dubravka; Marković, Snežana; Pavlović, Slađan; Žikić, Radoslav V.; Štajn, Andraš Š.; Saičić, Zorica

(Podgorica: Centre for Biodiversity of Montenegro, 2006)

TY  - CONF
AU  - Ognjanović, Branka
AU  - Filipović, Jasmina
AU  - Nikolić, Dubravka
AU  - Marković, Snežana
AU  - Pavlović, Slađan
AU  - Žikić, Radoslav V.
AU  - Štajn, Andraš Š.
AU  - Saičić, Zorica
PY  - 2006
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5966
AB  - Fish are the most important organisms used in biomonitoring of aquatic ecosystems.Parameters of oxidative stress in fish tissues represent significant biomarkers in theassessment of the status of environment. Specimens of hake (Merluccius merluccius L.) andsea bream (Pagellus erythrinus L.) were collected at the end of May 2005 from the locality infront of sea-port Bar (South Adriatic Sea). Parameters of oxidative stress (concentration oflipid peroxidase – LP and reduced glutathione – GSH) were determined in the liver and whitemuscle of hake and sea bream. Physical-chemical parameters (salinity, concentration ofdissolved oxygen and temperature) were determined in water of the investigated locality.Obtained results showed that the concentration of LP was higher in liver of hake and seabream in comparison to white muscle. However, significantly higher concentration of GSHwas found in white muscle of hake and sea bream in comparison to liver. These changes ofoxidative stress parameters point to tissue specificity, which is the consequence of differentmetabolic and antioxidative activity. Inter-specific differences were also established. Higherconcentrations of LP and GSH in liver of hake and in white muscle of sea bream wererecorded, due to changes of physical-chemical parameters of environment. The intensity ofoxidative stress in liver of hake and sea bream was higher in comparison to muscle.
PB  - Podgorica: Centre for Biodiversity of Montenegro
C3  - The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro
T1  - Lipid peroxidation and glutathione content in tissues of hake (Merluccius merluccius L.) and sea bream (Pagellus erythrinus L.) from the Adriatic sea
SP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5966
ER  - 
@conference{
author = "Ognjanović, Branka and Filipović, Jasmina and Nikolić, Dubravka and Marković, Snežana and Pavlović, Slađan and Žikić, Radoslav V. and Štajn, Andraš Š. and Saičić, Zorica",
year = "2006",
abstract = "Fish are the most important organisms used in biomonitoring of aquatic ecosystems.Parameters of oxidative stress in fish tissues represent significant biomarkers in theassessment of the status of environment. Specimens of hake (Merluccius merluccius L.) andsea bream (Pagellus erythrinus L.) were collected at the end of May 2005 from the locality infront of sea-port Bar (South Adriatic Sea). Parameters of oxidative stress (concentration oflipid peroxidase – LP and reduced glutathione – GSH) were determined in the liver and whitemuscle of hake and sea bream. Physical-chemical parameters (salinity, concentration ofdissolved oxygen and temperature) were determined in water of the investigated locality.Obtained results showed that the concentration of LP was higher in liver of hake and seabream in comparison to white muscle. However, significantly higher concentration of GSHwas found in white muscle of hake and sea bream in comparison to liver. These changes ofoxidative stress parameters point to tissue specificity, which is the consequence of differentmetabolic and antioxidative activity. Inter-specific differences were also established. Higherconcentrations of LP and GSH in liver of hake and in white muscle of sea bream wererecorded, due to changes of physical-chemical parameters of environment. The intensity ofoxidative stress in liver of hake and sea bream was higher in comparison to muscle.",
publisher = "Podgorica: Centre for Biodiversity of Montenegro",
journal = "The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro",
title = "Lipid peroxidation and glutathione content in tissues of hake (Merluccius merluccius L.) and sea bream (Pagellus erythrinus L.) from the Adriatic sea",
pages = "91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5966"
}
Ognjanović, B., Filipović, J., Nikolić, D., Marković, S., Pavlović, S., Žikić, R. V., Štajn, A. Š.,& Saičić, Z.. (2006). Lipid peroxidation and glutathione content in tissues of hake (Merluccius merluccius L.) and sea bream (Pagellus erythrinus L.) from the Adriatic sea. in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro
Podgorica: Centre for Biodiversity of Montenegro., 91.
https://hdl.handle.net/21.15107/rcub_ibiss_5966
Ognjanović B, Filipović J, Nikolić D, Marković S, Pavlović S, Žikić RV, Štajn AŠ, Saičić Z. Lipid peroxidation and glutathione content in tissues of hake (Merluccius merluccius L.) and sea bream (Pagellus erythrinus L.) from the Adriatic sea. in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro. 2006;:91.
https://hdl.handle.net/21.15107/rcub_ibiss_5966 .
Ognjanović, Branka, Filipović, Jasmina, Nikolić, Dubravka, Marković, Snežana, Pavlović, Slađan, Žikić, Radoslav V., Štajn, Andraš Š., Saičić, Zorica, "Lipid peroxidation and glutathione content in tissues of hake (Merluccius merluccius L.) and sea bream (Pagellus erythrinus L.) from the Adriatic sea" in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro (2006):91,
https://hdl.handle.net/21.15107/rcub_ibiss_5966 .

Alterations of energy metabolism and (anti)oxidant status in red blood cells of carps (Cyprinus carpio L.) exposed to acute hypoxia

Ognjanović, Branka; Marković, Snežana; Pavlović, Slađan; Žikić, Radoslav V.; Štajn, Andraš Š.; Saičić, Zorica S.

(Podgorica: Centre for Biodiversity of Montenegro, 2006)

TY  - CONF
AU  - Ognjanović, Branka
AU  - Marković, Snežana
AU  - Pavlović, Slađan
AU  - Žikić, Radoslav V.
AU  - Štajn, Andraš Š.
AU  - Saičić, Zorica S.
PY  - 2006
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5964
AB  - Specimens of the carps (Cyprinus carpio L.) were adapted to the oxygen concentration of 6.5mg/L water (100%) as control (C) group. The first (H1) and second (H2) experimental groupswere exposed to progressive reduction of O2 to 4.2 mg/L (55%) during 115 hours and 2.3 mg/L(30%) during 230 hours, respectively. Acute hypoxia decreased glucose (H1) and pyruvate (H1and H2) concentrations in the blood of carps, while lactate level was significantly increased(H2). Concentration of red blood cells (RBCs) ATP was decreased in both (H1 and H2)groups of fish. Concomitant increase of ADP concentration was observed in (H2) group offish. The decrease of ATP/ADP ratio in (H2) group was also found. In carps of (H1) group,the activities of superoxide dismutase (SOD) and catalase (CAT) in RBCs were increasedwhereas glutathione-S-transferase (GST) activity in the plasma was increased in both groupsof fish. Glutathione peroxidase (GSH-Px) activity and the concentration of reducedglutathione (GSH) were decreased in the same conditions. Increased concentration of lipidperoxides (LP) in the blood of (H1 and H2) groups of animals in relation to the controls wasalso found. In conclusion, acute hypoxia induced a significant alteration of antioxidantenzyme activities and lipid peroxide concentration in the blood of carps (Cyprinus carpio L.)under influence of progressive reduction of oxygen concentration. All specified changes aredependent from duration of hypoxic conditions. The presented results suggest that during the evolution carps developed a well defined metabolic regulation and antioxidant defense systemfor the protection from acute hypoxia.
PB  - Podgorica: Centre for Biodiversity of Montenegro
C3  - The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro
T1  - Alterations of energy metabolism and (anti)oxidant status in red blood cells of carps (Cyprinus carpio L.) exposed to acute hypoxia
SP  - 90
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5964
ER  - 
@conference{
author = "Ognjanović, Branka and Marković, Snežana and Pavlović, Slađan and Žikić, Radoslav V. and Štajn, Andraš Š. and Saičić, Zorica S.",
year = "2006",
abstract = "Specimens of the carps (Cyprinus carpio L.) were adapted to the oxygen concentration of 6.5mg/L water (100%) as control (C) group. The first (H1) and second (H2) experimental groupswere exposed to progressive reduction of O2 to 4.2 mg/L (55%) during 115 hours and 2.3 mg/L(30%) during 230 hours, respectively. Acute hypoxia decreased glucose (H1) and pyruvate (H1and H2) concentrations in the blood of carps, while lactate level was significantly increased(H2). Concentration of red blood cells (RBCs) ATP was decreased in both (H1 and H2)groups of fish. Concomitant increase of ADP concentration was observed in (H2) group offish. The decrease of ATP/ADP ratio in (H2) group was also found. In carps of (H1) group,the activities of superoxide dismutase (SOD) and catalase (CAT) in RBCs were increasedwhereas glutathione-S-transferase (GST) activity in the plasma was increased in both groupsof fish. Glutathione peroxidase (GSH-Px) activity and the concentration of reducedglutathione (GSH) were decreased in the same conditions. Increased concentration of lipidperoxides (LP) in the blood of (H1 and H2) groups of animals in relation to the controls wasalso found. In conclusion, acute hypoxia induced a significant alteration of antioxidantenzyme activities and lipid peroxide concentration in the blood of carps (Cyprinus carpio L.)under influence of progressive reduction of oxygen concentration. All specified changes aredependent from duration of hypoxic conditions. The presented results suggest that during the evolution carps developed a well defined metabolic regulation and antioxidant defense systemfor the protection from acute hypoxia.",
publisher = "Podgorica: Centre for Biodiversity of Montenegro",
journal = "The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro",
title = "Alterations of energy metabolism and (anti)oxidant status in red blood cells of carps (Cyprinus carpio L.) exposed to acute hypoxia",
pages = "90-91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5964"
}
Ognjanović, B., Marković, S., Pavlović, S., Žikić, R. V., Štajn, A. Š.,& Saičić, Z. S.. (2006). Alterations of energy metabolism and (anti)oxidant status in red blood cells of carps (Cyprinus carpio L.) exposed to acute hypoxia. in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro
Podgorica: Centre for Biodiversity of Montenegro., 90-91.
https://hdl.handle.net/21.15107/rcub_ibiss_5964
Ognjanović B, Marković S, Pavlović S, Žikić RV, Štajn AŠ, Saičić ZS. Alterations of energy metabolism and (anti)oxidant status in red blood cells of carps (Cyprinus carpio L.) exposed to acute hypoxia. in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro. 2006;:90-91.
https://hdl.handle.net/21.15107/rcub_ibiss_5964 .
Ognjanović, Branka, Marković, Snežana, Pavlović, Slađan, Žikić, Radoslav V., Štajn, Andraš Š., Saičić, Zorica S., "Alterations of energy metabolism and (anti)oxidant status in red blood cells of carps (Cyprinus carpio L.) exposed to acute hypoxia" in The Book of Abstracts and Programme: II International Symposium of Ecologists of Montenegro ISEM2; 2006 Sep 20-24; Kotor, Montenegro (2006):90-91,
https://hdl.handle.net/21.15107/rcub_ibiss_5964 .

Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river

Borković-Mitić, Slavica; Milošević, Slaviša; Radovanović, Tijana; Pavlović, Slađan; Štajn, Andraš Š.; Žikić, Radoslav V.; Maksimović-Kosanović, Katica; Saičić, Zorica

(Društvo fiziologa Republike Srbije, 2005)

TY  - CONF
AU  - Borković-Mitić, Slavica
AU  - Milošević, Slaviša
AU  - Radovanović, Tijana
AU  - Pavlović, Slađan
AU  - Štajn, Andraš Š.
AU  - Žikić, Radoslav V.
AU  - Maksimović-Kosanović, Katica
AU  - Saičić, Zorica
PY  - 2005
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5951
AB  - In our experiment we studied the antioxidant enzyme activities (superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase-GSH-Px, glutathione reductase-GR, and glutathione-S-transferase-GST} in hepatopancreas, gills and abdominal muscle of spinycheek crayfish (Orconectes limosus) from the Serbian part of Danube River. The speciments were collected with deep nets and tissue samples were prepared for analisys by standard methods. All enzyme activities were  measured spectrophotometrically and expressed as specific (U/mg proteins) and total (U/g net mass). The results show strong tissue specifity of investigated enzymes. Specific and total SOD activity in hepatopancreas was lower than in gills  and muscle. At the same time, total SOD activity in gills was significantly lower in respect to muscle. The specific and total activity of CAT was significantly higher in hepatopancreas than in gills and muscle, as well as in gills in respect to the muscle. Same trend was obtained for specific and total activity of GSH-Px. In gills of spinycheek crayfish·, specific activity of GR was significantly higher than in hepatopancreas and muscle, as well as in gills in respect to the muscle. Total GR activity was also significantly higher in gills comparing to hepatopancreas and muscle. The specific and total activity of GST was significantly increased in hepatopancreas than in gills and muscle. At the same time, the activity of GST was higher in gills than in muscle. The obtained results show a strong tissue specifity of antioxidant defense enzymes in investigated tissues of spinycheek crayfish which is accompanied with its different metabolic activity.
PB  - Društvo fiziologa Republike Srbije
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia
T1  - Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river
SP  - 219
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5951
ER  - 
@conference{
author = "Borković-Mitić, Slavica and Milošević, Slaviša and Radovanović, Tijana and Pavlović, Slađan and Štajn, Andraš Š. and Žikić, Radoslav V. and Maksimović-Kosanović, Katica and Saičić, Zorica",
year = "2005",
abstract = "In our experiment we studied the antioxidant enzyme activities (superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase-GSH-Px, glutathione reductase-GR, and glutathione-S-transferase-GST} in hepatopancreas, gills and abdominal muscle of spinycheek crayfish (Orconectes limosus) from the Serbian part of Danube River. The speciments were collected with deep nets and tissue samples were prepared for analisys by standard methods. All enzyme activities were  measured spectrophotometrically and expressed as specific (U/mg proteins) and total (U/g net mass). The results show strong tissue specifity of investigated enzymes. Specific and total SOD activity in hepatopancreas was lower than in gills  and muscle. At the same time, total SOD activity in gills was significantly lower in respect to muscle. The specific and total activity of CAT was significantly higher in hepatopancreas than in gills and muscle, as well as in gills in respect to the muscle. Same trend was obtained for specific and total activity of GSH-Px. In gills of spinycheek crayfish·, specific activity of GR was significantly higher than in hepatopancreas and muscle, as well as in gills in respect to the muscle. Total GR activity was also significantly higher in gills comparing to hepatopancreas and muscle. The specific and total activity of GST was significantly increased in hepatopancreas than in gills and muscle. At the same time, the activity of GST was higher in gills than in muscle. The obtained results show a strong tissue specifity of antioxidant defense enzymes in investigated tissues of spinycheek crayfish which is accompanied with its different metabolic activity.",
publisher = "Društvo fiziologa Republike Srbije, Belgrade: Serbian Physiology Society",
journal = "Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia",
title = "Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river",
pages = "219",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5951"
}
Borković-Mitić, S., Milošević, S., Radovanović, T., Pavlović, S., Štajn, A. Š., Žikić, R. V., Maksimović-Kosanović, K.,& Saičić, Z.. (2005). Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river. in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia
Društvo fiziologa Republike Srbije., 219.
https://hdl.handle.net/21.15107/rcub_ibiss_5951
Borković-Mitić S, Milošević S, Radovanović T, Pavlović S, Štajn AŠ, Žikić RV, Maksimović-Kosanović K, Saičić Z. Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river. in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia. 2005;:219.
https://hdl.handle.net/21.15107/rcub_ibiss_5951 .
Borković-Mitić, Slavica, Milošević, Slaviša, Radovanović, Tijana, Pavlović, Slađan, Štajn, Andraš Š., Žikić, Radoslav V., Maksimović-Kosanović, Katica, Saičić, Zorica, "Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river" in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia (2005):219,
https://hdl.handle.net/21.15107/rcub_ibiss_5951 .

Accumulation of cadmium in some tissues of rats after chronic treatment with CdCl2

Pavlović, Slađan; Ognjanović, Branka; Štajn, Andraš; Žikić, Radoslav; Petrović, Vojislav; Saičić, Zorica

(Belgrade: Serbian Physiology Society, 2005)

TY  - CONF
AU  - Pavlović, Slađan
AU  - Ognjanović, Branka
AU  - Štajn, Andraš
AU  - Žikić, Radoslav
AU  - Petrović, Vojislav
AU  - Saičić, Zorica
PY  - 2005
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5950
AB  - Cadmium is the important environmental pollutant and major routes of Cd intake are oral exposure and inhalation. From the total amount of food Cd intake, only about 6% is absorbed in the gastrointestinal tract of animals. The aim of our experiment was to determine the rate of Cd accumulation in liver, kidneys, skeletal muscle (musculus gastrocnemius) and testes of two months old Wistar albino male rats. Animals were divided into 2 experimental groups consisting 7 animals and treated in 30 days courses. The first group of animals functioned as controls (C) and the second group was treated with 200 mg CdCh x 5H20 in drinking water during 30 days (Cd). The average intake of 17 mg Cd/mg b.m. was calculated from the water consumed. Cadmium concentration was determined by atomic absorption spectrophotometry in the mixture of nitric and perchloric acid at the wavelength of 228.8 nm, slit 0.1 nm and lamp current 4 mA in the mixture of air and acetylene. The obtained results show a significant accumulation of Cd in all examined tissues of rats (p<0.005) in respect to controls. These results are in accordance with the findings of other authors and show that Cd mainly accumulated in the tissues of high metabolic rate, such as liver and kidneys. In these organs Cd accumulates mainly in the hepatocytes and cells of the proximal and distal renal tubules. In the cells Cd mainly deposits in the cytoplasm, nuclei and nucleoli where disturbs the cell metabolism, protein synthesis and sulfhydryl homeostasis.
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia
T1  - Accumulation of cadmium in some tissues of rats after chronic treatment with CdCl2
SP  - 191
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5950
ER  - 
@conference{
author = "Pavlović, Slađan and Ognjanović, Branka and Štajn, Andraš and Žikić, Radoslav and Petrović, Vojislav and Saičić, Zorica",
year = "2005",
abstract = "Cadmium is the important environmental pollutant and major routes of Cd intake are oral exposure and inhalation. From the total amount of food Cd intake, only about 6% is absorbed in the gastrointestinal tract of animals. The aim of our experiment was to determine the rate of Cd accumulation in liver, kidneys, skeletal muscle (musculus gastrocnemius) and testes of two months old Wistar albino male rats. Animals were divided into 2 experimental groups consisting 7 animals and treated in 30 days courses. The first group of animals functioned as controls (C) and the second group was treated with 200 mg CdCh x 5H20 in drinking water during 30 days (Cd). The average intake of 17 mg Cd/mg b.m. was calculated from the water consumed. Cadmium concentration was determined by atomic absorption spectrophotometry in the mixture of nitric and perchloric acid at the wavelength of 228.8 nm, slit 0.1 nm and lamp current 4 mA in the mixture of air and acetylene. The obtained results show a significant accumulation of Cd in all examined tissues of rats (p<0.005) in respect to controls. These results are in accordance with the findings of other authors and show that Cd mainly accumulated in the tissues of high metabolic rate, such as liver and kidneys. In these organs Cd accumulates mainly in the hepatocytes and cells of the proximal and distal renal tubules. In the cells Cd mainly deposits in the cytoplasm, nuclei and nucleoli where disturbs the cell metabolism, protein synthesis and sulfhydryl homeostasis.",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia",
title = "Accumulation of cadmium in some tissues of rats after chronic treatment with CdCl2",
pages = "191",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5950"
}
Pavlović, S., Ognjanović, B., Štajn, A., Žikić, R., Petrović, V.,& Saičić, Z.. (2005). Accumulation of cadmium in some tissues of rats after chronic treatment with CdCl2. in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia
Belgrade: Serbian Physiology Society., 191.
https://hdl.handle.net/21.15107/rcub_ibiss_5950
Pavlović S, Ognjanović B, Štajn A, Žikić R, Petrović V, Saičić Z. Accumulation of cadmium in some tissues of rats after chronic treatment with CdCl2. in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia. 2005;:191.
https://hdl.handle.net/21.15107/rcub_ibiss_5950 .
Pavlović, Slađan, Ognjanović, Branka, Štajn, Andraš, Žikić, Radoslav, Petrović, Vojislav, Saičić, Zorica, "Accumulation of cadmium in some tissues of rats after chronic treatment with CdCl2" in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia (2005):191,
https://hdl.handle.net/21.15107/rcub_ibiss_5950 .

Concentrations of vitamin E and sulphydryl group in some tissues of freshwater crayfish species

Radovanović, Tijana; Milošević, Slaviša; Pavlović, Slađan; Borković-Mitić, Slavica; Radojičić, Ratko; Žikić, Radoslav V.; Saičić, Zorica

(Belgrade: Serbian Physiology Society, 2005)

TY  - CONF
AU  - Radovanović, Tijana
AU  - Milošević, Slaviša
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Radojičić, Ratko
AU  - Žikić, Radoslav V.
AU  - Saičić, Zorica
PY  - 2005
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5952
AB  - The freshwater crayfishes can be useful organisms in the biomonitoring of environmental conditions. In our experiments we studied vitamin E (Vit E) and sulphydryl group (-SH)  concentrations  in  hepatopancreas,  gills  and  abdominal muscle of three crayfish species: Noble crayfish  (Astacus  astacus)  from  South  Morava river, Stone crayfish (Austropotamobius torrentium) from Krajkovacka river  and Spinycheek crayfish (Orconectes limosus) from Danube river. All species were collected in summer season (August). The  speciments  were  collected  by deep  nets and supematants for analysis were prepared according to the standard methods. Vit E concentration was assayed by standard method using bathophenantroline as reagent, while -SH groups concentration was  measured  by  standard  spectrophotometric method using dithionitrobenzoic acid (DTNB). The  obtained  results  show  significantly lower concentration of vit E in hepatopancreas of Orconectes limosus in respect to Astacus astacus (p<0.05). At the same time, significantly decreased concentration of vit E was detected in gills of Orconectes limosus comparing  to  Astacus astacus and Austropotamobius torrentium (p<0.005). Concentration of -SH groups was significantly increased  in  hepatopancreas  of  Noble  crayfish  in comparison to Stone crayfish (p<0.05) and Spinycheek crayfish (p<0.005). In conclusion,  the concentrations of the  investigated  antioxidant  compounds  (vit  E and
-SH groups) in the selected tissues of freshwater crayfishes are good markers for biomonitoring of environmental conditions. Differences in the investigated nonenzymatic oxidative stress parameters in tissues reflect different metabolic and antioxidative activities, which  can  be the consequence  of species  or tissue specifity,  as well as the consequence  of different environmental  conditions.
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia
T1  - Concentrations of vitamin E and sulphydryl group in some tissues of freshwater crayfish species
SP  - 190
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5952
ER  - 
@conference{
author = "Radovanović, Tijana and Milošević, Slaviša and Pavlović, Slađan and Borković-Mitić, Slavica and Radojičić, Ratko and Žikić, Radoslav V. and Saičić, Zorica",
year = "2005",
abstract = "The freshwater crayfishes can be useful organisms in the biomonitoring of environmental conditions. In our experiments we studied vitamin E (Vit E) and sulphydryl group (-SH)  concentrations  in  hepatopancreas,  gills  and  abdominal muscle of three crayfish species: Noble crayfish  (Astacus  astacus)  from  South  Morava river, Stone crayfish (Austropotamobius torrentium) from Krajkovacka river  and Spinycheek crayfish (Orconectes limosus) from Danube river. All species were collected in summer season (August). The  speciments  were  collected  by deep  nets and supematants for analysis were prepared according to the standard methods. Vit E concentration was assayed by standard method using bathophenantroline as reagent, while -SH groups concentration was  measured  by  standard  spectrophotometric method using dithionitrobenzoic acid (DTNB). The  obtained  results  show  significantly lower concentration of vit E in hepatopancreas of Orconectes limosus in respect to Astacus astacus (p<0.05). At the same time, significantly decreased concentration of vit E was detected in gills of Orconectes limosus comparing  to  Astacus astacus and Austropotamobius torrentium (p<0.005). Concentration of -SH groups was significantly increased  in  hepatopancreas  of  Noble  crayfish  in comparison to Stone crayfish (p<0.05) and Spinycheek crayfish (p<0.005). In conclusion,  the concentrations of the  investigated  antioxidant  compounds  (vit  E and
-SH groups) in the selected tissues of freshwater crayfishes are good markers for biomonitoring of environmental conditions. Differences in the investigated nonenzymatic oxidative stress parameters in tissues reflect different metabolic and antioxidative activities, which  can  be the consequence  of species  or tissue specifity,  as well as the consequence  of different environmental  conditions.",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia",
title = "Concentrations of vitamin E and sulphydryl group in some tissues of freshwater crayfish species",
pages = "190",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5952"
}
Radovanović, T., Milošević, S., Pavlović, S., Borković-Mitić, S., Radojičić, R., Žikić, R. V.,& Saičić, Z.. (2005). Concentrations of vitamin E and sulphydryl group in some tissues of freshwater crayfish species. in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia
Belgrade: Serbian Physiology Society., 190.
https://hdl.handle.net/21.15107/rcub_ibiss_5952
Radovanović T, Milošević S, Pavlović S, Borković-Mitić S, Radojičić R, Žikić RV, Saičić Z. Concentrations of vitamin E and sulphydryl group in some tissues of freshwater crayfish species. in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia. 2005;:190.
https://hdl.handle.net/21.15107/rcub_ibiss_5952 .
Radovanović, Tijana, Milošević, Slaviša, Pavlović, Slađan, Borković-Mitić, Slavica, Radojičić, Ratko, Žikić, Radoslav V., Saičić, Zorica, "Concentrations of vitamin E and sulphydryl group in some tissues of freshwater crayfish species" in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia (2005):190,
https://hdl.handle.net/21.15107/rcub_ibiss_5952 .

Effect of chronic cadmium exposure on glutathione peroxidase and glutathione s-transferase activities in rat: the role of selenium

Ognjanović, Branka; Marković, Snežana; Pavlović, Slađan; Žikić, Radoslav V.; Štajn, Andraš Š.; Petrović, Vojislav M.; Saičić, Zorica

(Belgrade: Serbian Physiology Society, 2005)

TY  - CONF
AU  - Ognjanović, Branka
AU  - Marković, Snežana
AU  - Pavlović, Slađan
AU  - Žikić, Radoslav V.
AU  - Štajn, Andraš Š.
AU  - Petrović, Vojislav M.
AU  - Saičić, Zorica
PY  - 2005
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5953
AB  - Cadmium (Cd) is an ubiquitous toxic metal that may induce oxidative damage by disturbing the prooxidant-antioxidant balance in the tissues. Among antioxidant micronutrients, selenium (Se) is an essential dietary trace element which ·plays an important role in a number of biological processess. The effect of cadmium on the activity of glutathione peroxidase (GSH-Px) and glutathione S-transferase (GST) which play an important role in the detoxification of xenobiotics, was studied in the liver, kidney and heart of rats. Furthermore, the role of selenium in the modulation of Cd toxicity with respect to GSH-Px and GST was also evaluated. In our experiments, cadmium exposure (15 mg Cd/kg body wt./day as CdCh for 4 weeks) to rats (n=30, male Wistar rats, age 8 weeks) resulted in decreased GSH-Px and GST activity in all organs  present  in  the  order  liver  >  kidney  >  heart.  Oral  administration  of  Se
(0.5 mg Se/kg body wt./day as Na2Se0 3 for 4 weeks) caused a significant increase in
 
IJ,,
Cl

It ra as
SC
at cc ef
ra w ar S1
Ir
 
GSH-Px activity in the order liver> heart> kidney. Selenium  administration caused	d,
an   increase   in  total  GST  activity  in  liver  but  a  decrease   in  kidney    and  heart.	Sf
Simultaneous  administration of Cd  and Se resulted  in  an  increase  GSH-Px and GST	p,
activity  in all  tissues  under study. Thus,  the mechanism  by which Se    decreases Cd	b,
toxicity in rats seems to rely on the protection of the enzyme systems   GSH-Px and	aJ GST in the three organs, possibly by forming non-toxic cadmium selenide. Selenium	th efficiently protects tissues from cadmium-induced oxidative damage
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia
T1  - Effect of chronic cadmium exposure on glutathione peroxidase and glutathione s-transferase activities in rat: the  role  of selenium
SP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5953
ER  - 
@conference{
author = "Ognjanović, Branka and Marković, Snežana and Pavlović, Slađan and Žikić, Radoslav V. and Štajn, Andraš Š. and Petrović, Vojislav M. and Saičić, Zorica",
year = "2005",
abstract = "Cadmium (Cd) is an ubiquitous toxic metal that may induce oxidative damage by disturbing the prooxidant-antioxidant balance in the tissues. Among antioxidant micronutrients, selenium (Se) is an essential dietary trace element which ·plays an important role in a number of biological processess. The effect of cadmium on the activity of glutathione peroxidase (GSH-Px) and glutathione S-transferase (GST) which play an important role in the detoxification of xenobiotics, was studied in the liver, kidney and heart of rats. Furthermore, the role of selenium in the modulation of Cd toxicity with respect to GSH-Px and GST was also evaluated. In our experiments, cadmium exposure (15 mg Cd/kg body wt./day as CdCh for 4 weeks) to rats (n=30, male Wistar rats, age 8 weeks) resulted in decreased GSH-Px and GST activity in all organs  present  in  the  order  liver  >  kidney  >  heart.  Oral  administration  of  Se
(0.5 mg Se/kg body wt./day as Na2Se0 3 for 4 weeks) caused a significant increase in
 
IJ,,
Cl

It ra as
SC
at cc ef
ra w ar S1
Ir
 
GSH-Px activity in the order liver> heart> kidney. Selenium  administration caused	d,
an   increase   in  total  GST  activity  in  liver  but  a  decrease   in  kidney    and  heart.	Sf
Simultaneous  administration of Cd  and Se resulted  in  an  increase  GSH-Px and GST	p,
activity  in all  tissues  under study. Thus,  the mechanism  by which Se    decreases Cd	b,
toxicity in rats seems to rely on the protection of the enzyme systems   GSH-Px and	aJ GST in the three organs, possibly by forming non-toxic cadmium selenide. Selenium	th efficiently protects tissues from cadmium-induced oxidative damage",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia",
title = "Effect of chronic cadmium exposure on glutathione peroxidase and glutathione s-transferase activities in rat: the  role  of selenium",
pages = "202",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5953"
}
Ognjanović, B., Marković, S., Pavlović, S., Žikić, R. V., Štajn, A. Š., Petrović, V. M.,& Saičić, Z.. (2005). Effect of chronic cadmium exposure on glutathione peroxidase and glutathione s-transferase activities in rat: the  role  of selenium. in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia
Belgrade: Serbian Physiology Society., 202.
https://hdl.handle.net/21.15107/rcub_ibiss_5953
Ognjanović B, Marković S, Pavlović S, Žikić RV, Štajn AŠ, Petrović VM, Saičić Z. Effect of chronic cadmium exposure on glutathione peroxidase and glutathione s-transferase activities in rat: the  role  of selenium. in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia. 2005;:202.
https://hdl.handle.net/21.15107/rcub_ibiss_5953 .
Ognjanović, Branka, Marković, Snežana, Pavlović, Slađan, Žikić, Radoslav V., Štajn, Andraš Š., Petrović, Vojislav M., Saičić, Zorica, "Effect of chronic cadmium exposure on glutathione peroxidase and glutathione s-transferase activities in rat: the  role  of selenium" in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia (2005):202,
https://hdl.handle.net/21.15107/rcub_ibiss_5953 .