TY  - JOUR
AU  - Devrnja, Nina
AU  - Anđelković, Boban
AU  - Ljujić, Jovana
AU  - Ćosić, Tatjana
AU  - Stupar, Sofija
AU  - Milutinović, Milica
AU  - Savić, Jelena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6632
AB  - β-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils
(FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation
method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and
monoterpenoids were the dominant chemical groups in total FEO, while in BEO, these two groups
occurred along with sesquiterpenes and sesquiterpenoids. The presence of dominant compounds
from both EOs was validated using the FT-IR spectra of ICs, which indicated successful complexation.
Analyses conducted using SPME/GC-MS showed the continuous emission of volatiles over 24 h from
both ICs. Under SEM, particles of both ICs appeared to have a rectangular or rhomboid morphology
and few aggregates. The insecticidal properties of EOs and ICs with β-CD were tested on the
Colorado potato beetle (CPB) as a model pest. The inclusion complex of β-CD with FEO altered the
developmental dynamic and body mass of the CPB. The initial increase in the proteolytic activity of
CPB larvae fed with potato plants sprayed with ICs was not maintained for long, and the proteolytic
efficacy of treated larvae remained in line with that of the control larvae. Future investigations will
focus on manipulating the volume of EOs used and the treatment duration for optimal efficacy and
potential application.
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation
IS  - 3
VL  - 14
DO  - 10.3390/biom14030353
SP  - 353
ER  - 

@article{
author = "Devrnja, Nina and Anđelković, Boban and Ljujić, Jovana and Ćosić, Tatjana and Stupar, Sofija and Milutinović, Milica and Savić, Jelena",
year = "2024",
abstract = "β-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils
(FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation
method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and
monoterpenoids were the dominant chemical groups in total FEO, while in BEO, these two groups
occurred along with sesquiterpenes and sesquiterpenoids. The presence of dominant compounds
from both EOs was validated using the FT-IR spectra of ICs, which indicated successful complexation.
Analyses conducted using SPME/GC-MS showed the continuous emission of volatiles over 24 h from
both ICs. Under SEM, particles of both ICs appeared to have a rectangular or rhomboid morphology
and few aggregates. The insecticidal properties of EOs and ICs with β-CD were tested on the
Colorado potato beetle (CPB) as a model pest. The inclusion complex of β-CD with FEO altered the
developmental dynamic and body mass of the CPB. The initial increase in the proteolytic activity of
CPB larvae fed with potato plants sprayed with ICs was not maintained for long, and the proteolytic
efficacy of treated larvae remained in line with that of the control larvae. Future investigations will
focus on manipulating the volume of EOs used and the treatment duration for optimal efficacy and
potential application.",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation",
number = "3",
volume = "14",
doi = "10.3390/biom14030353",
pages = "353"
}

Devrnja, N., Anđelković, B., Ljujić, J., Ćosić, T., Stupar, S., Milutinović, M.,& Savić, J.. (2024). Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation. in Biomolecules
Basel: MDPI., 14(3), 353.
https://doi.org/10.3390/biom14030353

Devrnja N, Anđelković B, Ljujić J, Ćosić T, Stupar S, Milutinović M, Savić J. Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation. in Biomolecules. 2024;14(3):353.
doi:10.3390/biom14030353 .

Devrnja, Nina, Anđelković, Boban, Ljujić, Jovana, Ćosić, Tatjana, Stupar, Sofija, Milutinović, Milica, Savić, Jelena, "Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation" in Biomolecules, 14, no. 3 (2024):353,
https://doi.org/10.3390/biom14030353 . .

TY  - JOUR
AU  - Pokimica, Nina
AU  - Ćosić, Tatjana
AU  - Uzelac, Branka
AU  - Ninković, Slavica
AU  - Raspor, Martin
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6631
AB  - Numerous biotechnological applications require a fast and efficient clonal propagation of whole plants under controlled laboratory conditions. For most plant species, the de novo regeneration of shoots from the cuttings of various plant organs can be obtained on nutrient media supplemented with plant hormones, auxin and cytokinin. While auxin is needed during the early stages of the process that include the establishment of pluripotent primordia and the subsequent acquisition of organogenic competence, cytokinin-supplemented media are required to induce these primordia to differentiate into developing shoots. The perception of cytokinin through the receptor ARABIDOPSIS HISTIDINE KINASE4 (AHK4) is crucial for the activation of the two main regulators of the establishment and maintenance of shoot apical meristems (SAMs): SHOOTMERISTEMLESS (STM) and the WUSCHEL-CLAVATA3 (WUS-CLV3) regulatory circuit. In this review, we summarize the current knowledge of the roles of the cytokinin signaling cascade in the perception and transduction of signals that are crucial for the de novo establishment of SAMs and lead to the desired biotechnological output—adventitious shoot multiplication. We highlight the functional differences between individual members of the multigene families involved in cytokinin signal transduction, and demonstrate how complex genetic regulation can be achieved through functional specialization of individual gene family members.
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation
IS  - 3
VL  - 14
DO  - 10.3390/biom14030381
SP  - 381
ER  - 

@article{
author = "Pokimica, Nina and Ćosić, Tatjana and Uzelac, Branka and Ninković, Slavica and Raspor, Martin",
year = "2024",
abstract = "Numerous biotechnological applications require a fast and efficient clonal propagation of whole plants under controlled laboratory conditions. For most plant species, the de novo regeneration of shoots from the cuttings of various plant organs can be obtained on nutrient media supplemented with plant hormones, auxin and cytokinin. While auxin is needed during the early stages of the process that include the establishment of pluripotent primordia and the subsequent acquisition of organogenic competence, cytokinin-supplemented media are required to induce these primordia to differentiate into developing shoots. The perception of cytokinin through the receptor ARABIDOPSIS HISTIDINE KINASE4 (AHK4) is crucial for the activation of the two main regulators of the establishment and maintenance of shoot apical meristems (SAMs): SHOOTMERISTEMLESS (STM) and the WUSCHEL-CLAVATA3 (WUS-CLV3) regulatory circuit. In this review, we summarize the current knowledge of the roles of the cytokinin signaling cascade in the perception and transduction of signals that are crucial for the de novo establishment of SAMs and lead to the desired biotechnological output—adventitious shoot multiplication. We highlight the functional differences between individual members of the multigene families involved in cytokinin signal transduction, and demonstrate how complex genetic regulation can be achieved through functional specialization of individual gene family members.",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation",
number = "3",
volume = "14",
doi = "10.3390/biom14030381",
pages = "381"
}

Pokimica, N., Ćosić, T., Uzelac, B., Ninković, S.,& Raspor, M.. (2024). Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation. in Biomolecules
Basel: MDPI., 14(3), 381.
https://doi.org/10.3390/biom14030381

Pokimica N, Ćosić T, Uzelac B, Ninković S, Raspor M. Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation. in Biomolecules. 2024;14(3):381.
doi:10.3390/biom14030381 .

Pokimica, Nina, Ćosić, Tatjana, Uzelac, Branka, Ninković, Slavica, Raspor, Martin, "Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation" in Biomolecules, 14, no. 3 (2024):381,
https://doi.org/10.3390/biom14030381 . .

TY  - JOUR
AU  - Dong, Shuqi
AU  - Fan, Min
AU  - Qin, Qiaoping
AU  - Zhang, Zhiguo
AU  - Duan, Ke
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Ni, Di-An
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6628
AB  - Mutant analysis remains one of the main genetic tools for characterising unclarified gene functions in plants, especially in non-model plants. Daylily (Hemerocallis spp.) is a popular perennial ornamental plant grown worldwide. Analysis of daylily mutants can enhance understanding of genes regulating the albino phenotype and improve the cultivar quality of daylily. The natural albino mutant (Alb−/−) was isolated by screening a self-pollinated progeny of daylily cultivar ‘black-eyed stella’. Transmission electron microscopy was used in analysing the structure of plastids between mutant and wild-type seedlings. The content of chlorophyll, carotenoids and chlorophyll precursors in plants was measured by ultraviolet spectrophotometry. RNA sequencing and physiological measurements were performed to explore the association between drought tolerance and mutation. All the seedlings of the daylily albino mutants died spontaneously within fifteen days after germination when grown in soil. The carotenoid and chlorophyll content in the leaves of the mutant plants significantly decreased compared with those of the wild-type control. The mutant plants displayed stunted growth, and their leaves were white or light yellow in color. Abnormal plastids such as those showing endomembrane vesiculation and lacking stacking were discovered in the leaves of mutant plants. Furthermore, genetic analysis revealed that a single recessive nuclear gene mutation led to the albino trait, RNA sequencing and real-time quantitative PCR validation showed extensive differences in gene expression between the mutant plants and the wild-type control, and most of the genes related to chlorophyll metabolism were down-regulated, with foldchange ranging from 0.20–0.49. Additionally, the surviving homozygous plants (Alb+/+), which do not contain this mutation, were also isolated by analysing the phenotype of their self-pollinated progeny. The net photosynthesis rate and light saturation point of Alb+/+ were higher than those of heterozygous (Alb+/−) plants. Additionally, the Alb+/+ plants were more tolerant to drought conditions than the Alb+/− plants, suggesting that a heterozygous Alb− mutation is sufficient to negatively affect photosynthetic efficiency and drought tolerance. The albino mutation negatively affects photosynthetic efficiency and drought tolerance, and homozygous mutation is required for the characteristic albino phenotype. This work highlights the link between albino mutation, photosynthetic pigment metabolism and drought sensitivity in daylily.
PB  - IMR Press
T2  - Frontiers in Bioscience - Landmark
T1  - Natural Albino Mutant of Daylily (Hemerocallis spp.) Reveals a Link between Drought Sensitivity and Photosynthetic Pigments Metabolism
IS  - 2
VL  - 29
DO  - 10.31083/j.fbl2902060
SP  - 60
ER  - 

@article{
author = "Dong, Shuqi and Fan, Min and Qin, Qiaoping and Zhang, Zhiguo and Duan, Ke and Ćosić, Tatjana and Raspor, Martin and Ni, Di-An",
year = "2024",
abstract = "Mutant analysis remains one of the main genetic tools for characterising unclarified gene functions in plants, especially in non-model plants. Daylily (Hemerocallis spp.) is a popular perennial ornamental plant grown worldwide. Analysis of daylily mutants can enhance understanding of genes regulating the albino phenotype and improve the cultivar quality of daylily. The natural albino mutant (Alb−/−) was isolated by screening a self-pollinated progeny of daylily cultivar ‘black-eyed stella’. Transmission electron microscopy was used in analysing the structure of plastids between mutant and wild-type seedlings. The content of chlorophyll, carotenoids and chlorophyll precursors in plants was measured by ultraviolet spectrophotometry. RNA sequencing and physiological measurements were performed to explore the association between drought tolerance and mutation. All the seedlings of the daylily albino mutants died spontaneously within fifteen days after germination when grown in soil. The carotenoid and chlorophyll content in the leaves of the mutant plants significantly decreased compared with those of the wild-type control. The mutant plants displayed stunted growth, and their leaves were white or light yellow in color. Abnormal plastids such as those showing endomembrane vesiculation and lacking stacking were discovered in the leaves of mutant plants. Furthermore, genetic analysis revealed that a single recessive nuclear gene mutation led to the albino trait, RNA sequencing and real-time quantitative PCR validation showed extensive differences in gene expression between the mutant plants and the wild-type control, and most of the genes related to chlorophyll metabolism were down-regulated, with foldchange ranging from 0.20–0.49. Additionally, the surviving homozygous plants (Alb+/+), which do not contain this mutation, were also isolated by analysing the phenotype of their self-pollinated progeny. The net photosynthesis rate and light saturation point of Alb+/+ were higher than those of heterozygous (Alb+/−) plants. Additionally, the Alb+/+ plants were more tolerant to drought conditions than the Alb+/− plants, suggesting that a heterozygous Alb− mutation is sufficient to negatively affect photosynthetic efficiency and drought tolerance. The albino mutation negatively affects photosynthetic efficiency and drought tolerance, and homozygous mutation is required for the characteristic albino phenotype. This work highlights the link between albino mutation, photosynthetic pigment metabolism and drought sensitivity in daylily.",
publisher = "IMR Press",
journal = "Frontiers in Bioscience - Landmark",
title = "Natural Albino Mutant of Daylily (Hemerocallis spp.) Reveals a Link between Drought Sensitivity and Photosynthetic Pigments Metabolism",
number = "2",
volume = "29",
doi = "10.31083/j.fbl2902060",
pages = "60"
}

Dong, S., Fan, M., Qin, Q., Zhang, Z., Duan, K., Ćosić, T., Raspor, M.,& Ni, D.. (2024). Natural Albino Mutant of Daylily (Hemerocallis spp.) Reveals a Link between Drought Sensitivity and Photosynthetic Pigments Metabolism. in Frontiers in Bioscience - Landmark
IMR Press., 29(2), 60.
https://doi.org/10.31083/j.fbl2902060

Dong S, Fan M, Qin Q, Zhang Z, Duan K, Ćosić T, Raspor M, Ni D. Natural Albino Mutant of Daylily (Hemerocallis spp.) Reveals a Link between Drought Sensitivity and Photosynthetic Pigments Metabolism. in Frontiers in Bioscience - Landmark. 2024;29(2):60.
doi:10.31083/j.fbl2902060 .

Dong, Shuqi, Fan, Min, Qin, Qiaoping, Zhang, Zhiguo, Duan, Ke, Ćosić, Tatjana, Raspor, Martin, Ni, Di-An, "Natural Albino Mutant of Daylily (Hemerocallis spp.) Reveals a Link between Drought Sensitivity and Photosynthetic Pigments Metabolism" in Frontiers in Bioscience - Landmark, 29, no. 2 (2024):60,
https://doi.org/10.31083/j.fbl2902060 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Anđela, Tomić
AU  - Uzelac, Branka
AU  - Raspor, Martin
AU  - Pokimica, Nina
AU  - Devrnja, Nina
AU  - Ninković, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6111
AB  - Deposition of carbohydrates in the lower part of the stem represents a unique feature of kohlrabi (Brassica
oleracea var. gongylodes), a member of Brassicaceae family grown for its high nutritional value. Over the
past decade our team intensely investigated in vitro development and regeneration of this significant crop,
determining, among other specific characteristics, an intricate relation between sucrose, serving as a carbon
source for in vitro growth, and cytokinins (CKs), utilized for the induction of indirect de novo shoot
organogenesis (DNSO). Even though the formation of kohlrabi stem tuber in vitro has not yet been
successfully attained, development of an efficient in vitro system for kohlrabi growth and DNSO opened the
possibility to study sugar metabolism in young kohlrabi plants, including the effects of elevated sucrose
application (6% and 9%) and CKs (trans-zeatin, transZ; thidiazuron, TDZ). Expression analysis of ten
selected genes involved in sugar partitioning in plants, indicated that both sucrose and CKs had similar
influence in the sense of downregulating these genes compared to control grown on plant growth
regulator-free medium with 3% sucrose. TDZ application had the highest effect on relative expression of all
three sucrose synthase genes (SUS1/3/6), particularly in three week-old plantlets (S1), the developmental
stage characterized by callus formation in the CK-treated kohlrabi. This trend was also observed for the
CWINV1, coding for β-fructofuranosidase and UGP1, coding for UTP--glucose-1-phosphate uridylyltransferase
1. On the other hand, high concentration of sucrose induced stronger downregulation of CINV1 (alkaline/
neutral invertase1), HXK1 (hexokinase1), GPI (glucose-6-phosphate isomerase) and SPS1 (sucrose-phosphate
synthase 1). TransZ led to small changes in gene expression, and even led to upregulation of SUS1 and
CINV1. Older plantlets (S2, four week-old, forming de novo shoots on CK treatment) were generally less
affected by applied treatments. In addition, histological sections of treated kohlrabi hypocotyls revealed
distinct changes at morpho-anatomical level. Our results, for the first time, depict effects of sucrose and CK
on activity of soluble sugars-related gene in young kohlrabi, suggesting an altered metabolism and transport
of soluble sugars, providing a starting point to the elucidation of stem tuber development and sugar
partitioning in kohlrabi.
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi
SP  - 345
EP  - 345
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6111
ER  - 

@conference{
author = "Ćosić, Tatjana and Anđela, Tomić and Uzelac, Branka and Raspor, Martin and Pokimica, Nina and Devrnja, Nina and Ninković, Slavica",
year = "2023",
abstract = "Deposition of carbohydrates in the lower part of the stem represents a unique feature of kohlrabi (Brassica
oleracea var. gongylodes), a member of Brassicaceae family grown for its high nutritional value. Over the
past decade our team intensely investigated in vitro development and regeneration of this significant crop,
determining, among other specific characteristics, an intricate relation between sucrose, serving as a carbon
source for in vitro growth, and cytokinins (CKs), utilized for the induction of indirect de novo shoot
organogenesis (DNSO). Even though the formation of kohlrabi stem tuber in vitro has not yet been
successfully attained, development of an efficient in vitro system for kohlrabi growth and DNSO opened the
possibility to study sugar metabolism in young kohlrabi plants, including the effects of elevated sucrose
application (6% and 9%) and CKs (trans-zeatin, transZ; thidiazuron, TDZ). Expression analysis of ten
selected genes involved in sugar partitioning in plants, indicated that both sucrose and CKs had similar
influence in the sense of downregulating these genes compared to control grown on plant growth
regulator-free medium with 3% sucrose. TDZ application had the highest effect on relative expression of all
three sucrose synthase genes (SUS1/3/6), particularly in three week-old plantlets (S1), the developmental
stage characterized by callus formation in the CK-treated kohlrabi. This trend was also observed for the
CWINV1, coding for β-fructofuranosidase and UGP1, coding for UTP--glucose-1-phosphate uridylyltransferase
1. On the other hand, high concentration of sucrose induced stronger downregulation of CINV1 (alkaline/
neutral invertase1), HXK1 (hexokinase1), GPI (glucose-6-phosphate isomerase) and SPS1 (sucrose-phosphate
synthase 1). TransZ led to small changes in gene expression, and even led to upregulation of SUS1 and
CINV1. Older plantlets (S2, four week-old, forming de novo shoots on CK treatment) were generally less
affected by applied treatments. In addition, histological sections of treated kohlrabi hypocotyls revealed
distinct changes at morpho-anatomical level. Our results, for the first time, depict effects of sucrose and CK
on activity of soluble sugars-related gene in young kohlrabi, suggesting an altered metabolism and transport
of soluble sugars, providing a starting point to the elucidation of stem tuber development and sugar
partitioning in kohlrabi.",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi",
pages = "345-345",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6111"
}

Ćosić, T., Anđela, T., Uzelac, B., Raspor, M., Pokimica, N., Devrnja, N.,& Ninković, S.. (2023). Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 345-345.
https://hdl.handle.net/21.15107/rcub_ibiss_6111

Ćosić T, Anđela T, Uzelac B, Raspor M, Pokimica N, Devrnja N, Ninković S. Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:345-345.
https://hdl.handle.net/21.15107/rcub_ibiss_6111 .

Ćosić, Tatjana, Anđela, Tomić, Uzelac, Branka, Raspor, Martin, Pokimica, Nina, Devrnja, Nina, Ninković, Slavica, "Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):345-345,
https://hdl.handle.net/21.15107/rcub_ibiss_6111 .

TY  - CONF
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Ćosić, Tatjana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Motyka, Vaclav
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6112
AB  - Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling
SP  - 484
EP  - 484
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6112
ER  - 

@conference{
author = "Ninković, Slavica and Banjac, Nevena and Stanišić, Mariana and Smailagić, Dijana and Ćosić, Tatjana and Živanović, Branka and Dobrev, Petre I. and Motyka, Vaclav",
year = "2023",
abstract = "Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling",
pages = "484-484",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6112"
}

Ninković, S., Banjac, N., Stanišić, M., Smailagić, D., Ćosić, T., Živanović, B., Dobrev, P. I.,& Motyka, V.. (2023). Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112

Ninković S, Banjac N, Stanišić M, Smailagić D, Ćosić T, Živanović B, Dobrev PI, Motyka V. Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

Ninković, Slavica, Banjac, Nevena, Stanišić, Mariana, Smailagić, Dijana, Ćosić, Tatjana, Živanović, Branka, Dobrev, Petre I., Motyka, Vaclav, "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):484-484,
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

TY  - CHAP
AU  - Raspor, Martin
AU  - Berić, Tanja
AU  - Ćosić, Tatjana
AU  - Stanković, Slaviša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6400
AB  - The rhizosphere is a microhabitat around plant roots that is actively created and regulated by plants, while having a major impact on plant life itself. Plants constantly enrich the rhizosphere with organic matter from their rhizodepositions and root exudates, shaping the chemical and microbiological composition of the space surrounding their roots. In this chapter, we discuss the interactions between plants and other organisms through the processes in the rhizosphere. Plant-associated microorganisms, such as plant growth-promoting bacteria and mycorrhizal fungi, play important roles in enhancing the survival of plants, through increasing the availability of nutrients from the soil to plants, degrading and immobilizing toxic compounds, alleviating the effects of abiotic stress, acting as biocontrol agents, protecting the plants from pathogens, and bolstering the efficiency of plant responses to biotic and abiotic stress. Moreover, plants use the rhizosphere as a battlefield for mutual competition by releasing allelochemicals that are detrimental to other plant species, and to harmful soil nematodes and insects. Similarly to land plants, aquatic plants also create a rhizosphere around their roots, with important implications for rice cultivation, methane emissions from wetlands, denitrification of aquatic habitats, and wastewater treatment through constructed wetlands. We emphasize how the existing and potential rhizosphere engineering approaches take advantage of the plant-associated microorganisms and allelopathic interactions between plants to improve the health and yield of agricultural crops, and help preserve the natural environment.
PB  - Cham: Springer
T2  - New Frontiers in Plant-Environment Interactions: Innovative Technologies and Developments
T1  - The Rhizosphere–A Hub of Emerging Importance for Plant Biotic Interactions
DO  - 10.1007/978-3-031-43729-8_11
SP  - 289
EP  - 327
ER  - 

@inbook{
author = "Raspor, Martin and Berić, Tanja and Ćosić, Tatjana and Stanković, Slaviša",
year = "2023",
abstract = "The rhizosphere is a microhabitat around plant roots that is actively created and regulated by plants, while having a major impact on plant life itself. Plants constantly enrich the rhizosphere with organic matter from their rhizodepositions and root exudates, shaping the chemical and microbiological composition of the space surrounding their roots. In this chapter, we discuss the interactions between plants and other organisms through the processes in the rhizosphere. Plant-associated microorganisms, such as plant growth-promoting bacteria and mycorrhizal fungi, play important roles in enhancing the survival of plants, through increasing the availability of nutrients from the soil to plants, degrading and immobilizing toxic compounds, alleviating the effects of abiotic stress, acting as biocontrol agents, protecting the plants from pathogens, and bolstering the efficiency of plant responses to biotic and abiotic stress. Moreover, plants use the rhizosphere as a battlefield for mutual competition by releasing allelochemicals that are detrimental to other plant species, and to harmful soil nematodes and insects. Similarly to land plants, aquatic plants also create a rhizosphere around their roots, with important implications for rice cultivation, methane emissions from wetlands, denitrification of aquatic habitats, and wastewater treatment through constructed wetlands. We emphasize how the existing and potential rhizosphere engineering approaches take advantage of the plant-associated microorganisms and allelopathic interactions between plants to improve the health and yield of agricultural crops, and help preserve the natural environment.",
publisher = "Cham: Springer",
journal = "New Frontiers in Plant-Environment Interactions: Innovative Technologies and Developments",
booktitle = "The Rhizosphere–A Hub of Emerging Importance for Plant Biotic Interactions",
doi = "10.1007/978-3-031-43729-8_11",
pages = "289-327"
}

Raspor, M., Berić, T., Ćosić, T.,& Stanković, S.. (2023). The Rhizosphere–A Hub of Emerging Importance for Plant Biotic Interactions. in New Frontiers in Plant-Environment Interactions: Innovative Technologies and Developments
Cham: Springer., 289-327.
https://doi.org/10.1007/978-3-031-43729-8_11

Raspor M, Berić T, Ćosić T, Stanković S. The Rhizosphere–A Hub of Emerging Importance for Plant Biotic Interactions. in New Frontiers in Plant-Environment Interactions: Innovative Technologies and Developments. 2023;:289-327.
doi:10.1007/978-3-031-43729-8_11 .

Raspor, Martin, Berić, Tanja, Ćosić, Tatjana, Stanković, Slaviša, "The Rhizosphere–A Hub of Emerging Importance for Plant Biotic Interactions" in New Frontiers in Plant-Environment Interactions: Innovative Technologies and Developments (2023):289-327,
https://doi.org/10.1007/978-3-031-43729-8_11 . .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6372
AB  - French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.
PB  - Springer Nature
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil
DO  - 10.1007/s00344-023-11131-8
ER  - 

@article{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2023",
abstract = "French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.",
publisher = "Springer Nature",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil",
doi = "10.1007/s00344-023-11131-8"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2023). Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation
Springer Nature..
https://doi.org/10.1007/s00344-023-11131-8

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation. 2023;.
doi:10.1007/s00344-023-11131-8 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil" in Journal of Plant Growth Regulation (2023),
https://doi.org/10.1007/s00344-023-11131-8 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Motyka, Vaclav
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5856
AB  - Kohlrabi (Brassica oleracea var. gongylodes) is a vegetable cultivated for its edible stem tuber. Although valued for its nutritional properties and tolerance to abiotic stress, kohlrabi is one of the least studied brassicas. In this review, we summarize the results of our decade-long research on in vitro morphogenesis of kohlrabi, starting from 2013. Protocols for efficient in vitro regeneration with minimal requirements for external application of plant growth regulators (PGRs) have been developed, both by somatic embryogenesis (SE) and by de novo shoot organogenesis (DNSO). Efficient regeneration by direct SE was achieved using immature zygotic embryos as explants incubated on PGR-free media, and the production process was maintained in culture thanks to highly efficient secondary SE. Conversely, efficient regeneration by indirect DNSO was achieved using entire seedlings as explants and adding only cytokinin (CK) without the need for exogenous auxin. Comprehensive phytohormone analyses revealed that different exogenously applied CKs differentially affected the composition of endogenous phytohormones and induced changes in the expression of cell cycle-related genes and other genes involved in the organogenic response. The addition of high sucrose concentrations to the nutrient media failed to induce the formation of stem tubers in in vitro culture, but revealed a complex interaction with exogenously applied CKs, interfering with both the endogenous phytohormonome and the expression of organogenesis-related genes. Our work has provided substantial biotechnological advances in the field of in vitro regeneration of kohlrabi, as well as in understanding the underlying phytohormonal regulation. The review aims to introduce kohlrabi to the scientific community as a model system for both basic and applied research, while we continue seeking answers to the outstanding questions and trying to pave the way for the development of more resistant varieties.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research
IS  - 6
VL  - 9
DO  - 10.3390/horticulturae9060674
SP  - 674
ER  - 

@article{
author = "Ćosić, Tatjana and Raspor, Martin and Motyka, Vaclav and Cingel, Aleksandar and Ninković, Slavica",
year = "2023",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) is a vegetable cultivated for its edible stem tuber. Although valued for its nutritional properties and tolerance to abiotic stress, kohlrabi is one of the least studied brassicas. In this review, we summarize the results of our decade-long research on in vitro morphogenesis of kohlrabi, starting from 2013. Protocols for efficient in vitro regeneration with minimal requirements for external application of plant growth regulators (PGRs) have been developed, both by somatic embryogenesis (SE) and by de novo shoot organogenesis (DNSO). Efficient regeneration by direct SE was achieved using immature zygotic embryos as explants incubated on PGR-free media, and the production process was maintained in culture thanks to highly efficient secondary SE. Conversely, efficient regeneration by indirect DNSO was achieved using entire seedlings as explants and adding only cytokinin (CK) without the need for exogenous auxin. Comprehensive phytohormone analyses revealed that different exogenously applied CKs differentially affected the composition of endogenous phytohormones and induced changes in the expression of cell cycle-related genes and other genes involved in the organogenic response. The addition of high sucrose concentrations to the nutrient media failed to induce the formation of stem tubers in in vitro culture, but revealed a complex interaction with exogenously applied CKs, interfering with both the endogenous phytohormonome and the expression of organogenesis-related genes. Our work has provided substantial biotechnological advances in the field of in vitro regeneration of kohlrabi, as well as in understanding the underlying phytohormonal regulation. The review aims to introduce kohlrabi to the scientific community as a model system for both basic and applied research, while we continue seeking answers to the outstanding questions and trying to pave the way for the development of more resistant varieties.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research",
number = "6",
volume = "9",
doi = "10.3390/horticulturae9060674",
pages = "674"
}

Ćosić, T., Raspor, M., Motyka, V., Cingel, A.,& Ninković, S.. (2023). In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research. in Horticulturae
Basel: MDPI., 9(6), 674.
https://doi.org/10.3390/horticulturae9060674

Ćosić T, Raspor M, Motyka V, Cingel A, Ninković S. In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research. in Horticulturae. 2023;9(6):674.
doi:10.3390/horticulturae9060674 .

Ćosić, Tatjana, Raspor, Martin, Motyka, Vaclav, Cingel, Aleksandar, Ninković, Slavica, "In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research" in Horticulturae, 9, no. 6 (2023):674,
https://doi.org/10.3390/horticulturae9060674 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Krstić-Milošević, Dijana
AU  - Mijalković, Tatjana
AU  - Petrović, Mirjana
AU  - Ćosić, Tatjana
AU  - Stanišić, Mariana
AU  - Vinterhalter, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5698
AB  - Eruca sativa is known in traditional medicine for its therapeutic effects, while young plants
are used as a salad or green food. Recently, the consumption of rocket has increased considerably,
so it has become very important for breeders. Plant tissue culture provides a platform to overcome
the problems in improving this species. In the present study, an efficient protocol for in vitro shoot
regeneration and propagation of recalcitrant rocket variety Domaća rukola was studied. Murashige
and Skoog (MS, 1962) medium containing 0.1 mg/L BA and frequent subculture over a period
of three weeks proved to be optimal for shoot multiplication with a multiplication index of over 3
and only 8.72% of hyperhydrated shoots without necrosis. Different concentrations of 2,4-D, BA, or
TDZ in combination with NAA, with or without the presence of AgNO3, were tested for de novo
shoot organogenesis (DNSO) from seedling explants. The hypocotyl explants cultured on MS with a
combination of TDZ1.0+NAA0.1+AgNO3 5.0 mg/L regenerated viable shoots with the highest rate
(25.38%) and an average number of 2.18 shoots per regenerating explant. Somatic embryogenesis
from immature zygotic embryos proved to be the best way to regenerate a recalcitrant rocket cultivar.
The highest embryogenic efficiency was achieved in explants cultured on MS medium containing
1.0 mg/L 2,4-D with a frequency of 76.64% and 5.13 mean number of regenerated somatic embryos
per explant, which were further converted into normal plants. Additionally, in vitro-produced rocket
shoots could serve as a possible promising source for the production of flavonoid kaempferol with
proven antioxidant properties.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola
IS  - 5
VL  - 9
DO  - 10.3390/horticulturae9050533
SP  - 533
ER  - 

@article{
author = "Banjac, Nevena and Krstić-Milošević, Dijana and Mijalković, Tatjana and Petrović, Mirjana and Ćosić, Tatjana and Stanišić, Mariana and Vinterhalter, Branka",
year = "2023",
abstract = "Eruca sativa is known in traditional medicine for its therapeutic effects, while young plants
are used as a salad or green food. Recently, the consumption of rocket has increased considerably,
so it has become very important for breeders. Plant tissue culture provides a platform to overcome
the problems in improving this species. In the present study, an efficient protocol for in vitro shoot
regeneration and propagation of recalcitrant rocket variety Domaća rukola was studied. Murashige
and Skoog (MS, 1962) medium containing 0.1 mg/L BA and frequent subculture over a period
of three weeks proved to be optimal for shoot multiplication with a multiplication index of over 3
and only 8.72% of hyperhydrated shoots without necrosis. Different concentrations of 2,4-D, BA, or
TDZ in combination with NAA, with or without the presence of AgNO3, were tested for de novo
shoot organogenesis (DNSO) from seedling explants. The hypocotyl explants cultured on MS with a
combination of TDZ1.0+NAA0.1+AgNO3 5.0 mg/L regenerated viable shoots with the highest rate
(25.38%) and an average number of 2.18 shoots per regenerating explant. Somatic embryogenesis
from immature zygotic embryos proved to be the best way to regenerate a recalcitrant rocket cultivar.
The highest embryogenic efficiency was achieved in explants cultured on MS medium containing
1.0 mg/L 2,4-D with a frequency of 76.64% and 5.13 mean number of regenerated somatic embryos
per explant, which were further converted into normal plants. Additionally, in vitro-produced rocket
shoots could serve as a possible promising source for the production of flavonoid kaempferol with
proven antioxidant properties.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola",
number = "5",
volume = "9",
doi = "10.3390/horticulturae9050533",
pages = "533"
}

Banjac, N., Krstić-Milošević, D., Mijalković, T., Petrović, M., Ćosić, T., Stanišić, M.,& Vinterhalter, B.. (2023). In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola. in Horticulturae
Basel: MDPI., 9(5), 533.
https://doi.org/10.3390/horticulturae9050533

Banjac N, Krstić-Milošević D, Mijalković T, Petrović M, Ćosić T, Stanišić M, Vinterhalter B. In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola. in Horticulturae. 2023;9(5):533.
doi:10.3390/horticulturae9050533 .

Banjac, Nevena, Krstić-Milošević, Dijana, Mijalković, Tatjana, Petrović, Mirjana, Ćosić, Tatjana, Stanišić, Mariana, Vinterhalter, Branka, "In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola" in Horticulturae, 9, no. 5 (2023):533,
https://doi.org/10.3390/horticulturae9050533 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Krstić Milošević, Dijana
AU  - Božić, Dragana
AU  - Jančić, Ivan
AU  - Raspor, Martin
AU  - Maksimović, Zoran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5365
AB  - In Serbia, citral chemotype of Thymus pannonicus All. (Lamiaceae) can be only found on Mt
Vršačke planine, as dominant form, where is traditionally used for medicinal purposes. To overcome
difficulties in harvesting due to distribution in inaccessible areas, and to prevent possible
overexploitation from its natural habitat, finding the means for alternative cultivation of this plant
for its prospective beneficial use in human health care was set as objective of this study. Efficient
system for in vitro shoot propagation of T. pannonicus was established for the first time. Different
types of auxin (indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; 1-naphthaleneacetic acid, NAA)
were used for testing the process of rhizogenesis in vitro. Best response was detected on IBA and
NAA for rhizogenesis frequency and number of formed roots, respectively. Rooted plants were
successfully acclimatized. In vitro shoots cultivated on PGR free medium (E1) and medium with 1
mg L-1 IAA (E2) were extracted with 80% methanol and analysed by HPLC. Presence of rosmarinic
acid (RA) as dominant component was indicated in both extracts, with E2 containing higher RA
amount (45.116 mg g-1DW). Antimicrobial activity of extracts was tested using broth microdilution
method against nine strains of laboratory control pathogenic microorganisms. Both extracts
exerted significant antimicrobial activity against all tested pathogens with minimal inhibitory concen-
trations (MIC) in the range of 6.25-50 mg mL-1, while E2 was more effective against Gram positive
bacteria (6.25-12.5 mg mL-1). Presented results could give inducement for potential greater
commercial use of this significant species in the future.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Potential of Thymus pannonicus All. in vitro culture: micropropagation, rosmarinic acid production and antimicrobial activity
SP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5365
ER  - 

@conference{
author = "Ćosić, Tatjana and Devrnja, Nina and Krstić Milošević, Dijana and Božić, Dragana and Jančić, Ivan and Raspor, Martin and Maksimović, Zoran",
year = "2022",
abstract = "In Serbia, citral chemotype of Thymus pannonicus All. (Lamiaceae) can be only found on Mt
Vršačke planine, as dominant form, where is traditionally used for medicinal purposes. To overcome
difficulties in harvesting due to distribution in inaccessible areas, and to prevent possible
overexploitation from its natural habitat, finding the means for alternative cultivation of this plant
for its prospective beneficial use in human health care was set as objective of this study. Efficient
system for in vitro shoot propagation of T. pannonicus was established for the first time. Different
types of auxin (indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; 1-naphthaleneacetic acid, NAA)
were used for testing the process of rhizogenesis in vitro. Best response was detected on IBA and
NAA for rhizogenesis frequency and number of formed roots, respectively. Rooted plants were
successfully acclimatized. In vitro shoots cultivated on PGR free medium (E1) and medium with 1
mg L-1 IAA (E2) were extracted with 80% methanol and analysed by HPLC. Presence of rosmarinic
acid (RA) as dominant component was indicated in both extracts, with E2 containing higher RA
amount (45.116 mg g-1DW). Antimicrobial activity of extracts was tested using broth microdilution
method against nine strains of laboratory control pathogenic microorganisms. Both extracts
exerted significant antimicrobial activity against all tested pathogens with minimal inhibitory concen-
trations (MIC) in the range of 6.25-50 mg mL-1, while E2 was more effective against Gram positive
bacteria (6.25-12.5 mg mL-1). Presented results could give inducement for potential greater
commercial use of this significant species in the future.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Potential of Thymus pannonicus All. in vitro culture: micropropagation, rosmarinic acid production and antimicrobial activity",
pages = "104",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5365"
}

Ćosić, T., Devrnja, N., Krstić Milošević, D., Božić, D., Jančić, I., Raspor, M.,& Maksimović, Z.. (2022). Potential of Thymus pannonicus All. in vitro culture: micropropagation, rosmarinic acid production and antimicrobial activity. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 104.
https://hdl.handle.net/21.15107/rcub_ibiss_5365

Ćosić T, Devrnja N, Krstić Milošević D, Božić D, Jančić I, Raspor M, Maksimović Z. Potential of Thymus pannonicus All. in vitro culture: micropropagation, rosmarinic acid production and antimicrobial activity. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:104.
https://hdl.handle.net/21.15107/rcub_ibiss_5365 .

Ćosić, Tatjana, Devrnja, Nina, Krstić Milošević, Dijana, Božić, Dragana, Jančić, Ivan, Raspor, Martin, Maksimović, Zoran, "Potential of Thymus pannonicus All. in vitro culture: micropropagation, rosmarinic acid production and antimicrobial activity" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):104,
https://hdl.handle.net/21.15107/rcub_ibiss_5365 .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Raspor, Martin
AU  - Sajid, Sumbal
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5364
AB  - This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5364
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Václav and Raspor, Martin and Sajid, Sumbal and Savić, Jelena and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2022",
abstract = "This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5364"
}

Ćosić, T., Motyka, V., Raspor, M., Sajid, S., Savić, J., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2022). Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364

Ćosić T, Motyka V, Raspor M, Sajid S, Savić J, Devrnja N, Dobrev PI, Ninković S. Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

Ćosić, Tatjana, Motyka, Václav, Raspor, Martin, Sajid, Sumbal, Savić, Jelena, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):24,
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

TY  - CONF
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5049
AB  - French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
T1  - Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5049
ER  - 

@conference{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022",
title = "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5049"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2022). Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
Belgrade: Serbian Plant Physiology Society., 76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022. 2022;:76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil" in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022 (2022):76,
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Raspor, Martin
AU  - Sajid, Sumbal
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5117
AB  - The establishment of an efficient protocol for in vitro growth and regeneration of kohlrabi (Brassica oleracea var. gongylodes) allowed us to closely examine the phytohormone profiles of kohlrabi seedlings at four growth stages (T1–T4), additionally including the effects of cytokinins (CKs)—trans-zeatin (transZ) and thidiazuron (TDZ)—and high sucrose concentrations (6% and 9%). Resulting phytohormone profiles showed complex time-course patterns. At the T2 stage of control kohlrabi plantlets (with two emerged true leaves), levels of endogenous CK free bases and gibberellin GA20 increased, while increases in jasmonic acid (JA), JA-isoleucine (JA-Ile), indole-3-acetic acid (IAA) and indole-3-acetamide (IAM) peaked later, at T3. At the same time, the content of most of the analyzed IAA metabolites decreased. Supplementing growth media with CK induced de novo formation of shoots, while both CK and sucrose treatments caused important changes in most of the phytohormone groups at each developmental stage, compared to control. Principal component analysis (PCA) showed that sucrose treatment, especially at 9%, had a stronger effect on the content of endogenous hormones than CK treatments. Correlation analysis showed that the dynamic balance between the levels of certain bioactive phytohormone forms and some of their metabolites could be lost or reversed at particular growth stages and under certain CK or sucrose treatments, with correlation values changing between strongly positive and strongly negative. Our results indicate that the kohlrabi phytohormonome is a highly dynamic system that changes greatly along the developmental time scale and also during de novo shoot formation, depending on exogenous factors such as the presence of growth regulators and different sucrose concentrations in the growth media, and that it interacts intensively with these factors to facilitate certain responses.
PB  - Basel: MDPI
T2  - Life
T1  - Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose
IS  - 10
VL  - 12
DO  - 10.3390/life12101585
SP  - 1585
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Vaclav and Raspor, Martin and Sajid, Sumbal and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2022",
abstract = "The establishment of an efficient protocol for in vitro growth and regeneration of kohlrabi (Brassica oleracea var. gongylodes) allowed us to closely examine the phytohormone profiles of kohlrabi seedlings at four growth stages (T1–T4), additionally including the effects of cytokinins (CKs)—trans-zeatin (transZ) and thidiazuron (TDZ)—and high sucrose concentrations (6% and 9%). Resulting phytohormone profiles showed complex time-course patterns. At the T2 stage of control kohlrabi plantlets (with two emerged true leaves), levels of endogenous CK free bases and gibberellin GA20 increased, while increases in jasmonic acid (JA), JA-isoleucine (JA-Ile), indole-3-acetic acid (IAA) and indole-3-acetamide (IAM) peaked later, at T3. At the same time, the content of most of the analyzed IAA metabolites decreased. Supplementing growth media with CK induced de novo formation of shoots, while both CK and sucrose treatments caused important changes in most of the phytohormone groups at each developmental stage, compared to control. Principal component analysis (PCA) showed that sucrose treatment, especially at 9%, had a stronger effect on the content of endogenous hormones than CK treatments. Correlation analysis showed that the dynamic balance between the levels of certain bioactive phytohormone forms and some of their metabolites could be lost or reversed at particular growth stages and under certain CK or sucrose treatments, with correlation values changing between strongly positive and strongly negative. Our results indicate that the kohlrabi phytohormonome is a highly dynamic system that changes greatly along the developmental time scale and also during de novo shoot formation, depending on exogenous factors such as the presence of growth regulators and different sucrose concentrations in the growth media, and that it interacts intensively with these factors to facilitate certain responses.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose",
number = "10",
volume = "12",
doi = "10.3390/life12101585",
pages = "1585"
}

Ćosić, T., Motyka, V., Raspor, M., Sajid, S., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2022). Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose. in Life
Basel: MDPI., 12(10), 1585.
https://doi.org/10.3390/life12101585

Ćosić T, Motyka V, Raspor M, Sajid S, Devrnja N, Dobrev PI, Ninković S. Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose. in Life. 2022;12(10):1585.
doi:10.3390/life12101585 .

Ćosić, Tatjana, Motyka, Vaclav, Raspor, Martin, Sajid, Sumbal, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose" in Life, 12, no. 10 (2022):1585,
https://doi.org/10.3390/life12101585 . .

TY  - JOUR
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Pěnčík, Aleš
AU  - Ćalić, Dušica
AU  - Bogdanović, Milica
AU  - Trajković, Milena
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5065
AB  - Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action
VL  - 13
DO  - 0.3389/fpls.2022.875528
SP  - 875528
ER  - 

@article{
author = "Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Pěnčík, Aleš and Ćalić, Dušica and Bogdanović, Milica and Trajković, Milena and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action",
volume = "13",
doi = "0.3389/fpls.2022.875528",
pages = "875528"
}

Smailagić, D., Banjac, N., Ninković, S., Savić, J., Ćosić, T., Pěnčík, A., Ćalić, D., Bogdanović, M., Trajković, M.,& Stanišić, M.. (2022). New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 13, 875528.
https://doi.org/0.3389/fpls.2022.875528

Smailagić D, Banjac N, Ninković S, Savić J, Ćosić T, Pěnčík A, Ćalić D, Bogdanović M, Trajković M, Stanišić M. New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science. 2022;13:875528.
doi:0.3389/fpls.2022.875528 .

Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Pěnčík, Aleš, Ćalić, Dušica, Bogdanović, Milica, Trajković, Milena, Stanišić, Mariana, "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action" in Frontiers in Plant Science, 13 (2022):875528,
https://doi.org/0.3389/fpls.2022.875528 . .

TY  - JOUR
AU  - Napar, Wado Photo Faqir
AU  - Kaleri, Abdul Rasheed
AU  - Ahmed, Awais
AU  - Nabi, Farhan
AU  - Sajid, Sumbal
AU  - Ćosić, Tatjana
AU  - Yao, Yinan
AU  - Liu, Jikai
AU  - Raspor, Martin
AU  - Gao, Yongfeng
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4869
AB  - Tomato cultivation is affected by high soil salinity and drought stress, which cause major yield losses worldwide. In this work, we compare the efficiency of mechanisms of tolerance to salinity, and osmotic stress applied as mannitol or drought, in three tomato genotypes: LA-2838 (Ailsa Craig), LA-2662 (Saladette), and LA-1996 (Anthocyanin  fruit  – Aft),  a  genotype  known  for  high  anthocyanin  content. Exposure  to  salinity  or   drought induced  stress  in  all three  genotypes,  but the  LA-1996 plants displayed  superior tolerance to stress compared with the other two genotypes. They were more efficient in anthocyanin and proline accumulation, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity, and leaf Na+, K+, and Ca2+ homeostasis. In addition, they suffered lesser oxidative damage as measured by chlorophyll (Chl) loss and malondialdehyde (MDA) accumulation, and bioassays showed that they were less affected in terms of seed germination and root elongation. Exposure to stress induced the upregulation of stress-related genes SlNCED1, SlAREB1, SlABF4, SlWRKY8, and SlDREB2A more efficiently in LA-1996 than in the two susceptible genotypes. Conversely, the upregulation of the NADPH oxidase gene SlRBOH1 was more pronounced in LA-2838 and LA-2662. Principal component analysis showed obvious distinction between the tolerant genotype LA-1996 and the susceptible LA- 2838 and LA-2662 in response to stress, and association of leaf and stem anthocyanin content with major stress tolerance traits. We suggest that anthocyanin accumulation can be considered as a marker of stress tolerance in tomato, and that LA-1996 can be considered for cultivation in salinity- or drought-affected areas.
PB  - Elsevier GmbH
T2  - Journal of Plant Physiology
T1  - The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought
IS  - 271
DO  - 10.1016/j.jplph.2022.153662
SP  - 153662
ER  - 

@article{
author = "Napar, Wado Photo Faqir and Kaleri, Abdul Rasheed and Ahmed, Awais and Nabi, Farhan and Sajid, Sumbal and Ćosić, Tatjana and Yao, Yinan and Liu, Jikai and Raspor, Martin and Gao, Yongfeng",
year = "2022",
abstract = "Tomato cultivation is affected by high soil salinity and drought stress, which cause major yield losses worldwide. In this work, we compare the efficiency of mechanisms of tolerance to salinity, and osmotic stress applied as mannitol or drought, in three tomato genotypes: LA-2838 (Ailsa Craig), LA-2662 (Saladette), and LA-1996 (Anthocyanin  fruit  – Aft),  a  genotype  known  for  high  anthocyanin  content. Exposure  to  salinity  or   drought induced  stress  in  all three  genotypes,  but the  LA-1996 plants displayed  superior tolerance to stress compared with the other two genotypes. They were more efficient in anthocyanin and proline accumulation, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity, and leaf Na+, K+, and Ca2+ homeostasis. In addition, they suffered lesser oxidative damage as measured by chlorophyll (Chl) loss and malondialdehyde (MDA) accumulation, and bioassays showed that they were less affected in terms of seed germination and root elongation. Exposure to stress induced the upregulation of stress-related genes SlNCED1, SlAREB1, SlABF4, SlWRKY8, and SlDREB2A more efficiently in LA-1996 than in the two susceptible genotypes. Conversely, the upregulation of the NADPH oxidase gene SlRBOH1 was more pronounced in LA-2838 and LA-2662. Principal component analysis showed obvious distinction between the tolerant genotype LA-1996 and the susceptible LA- 2838 and LA-2662 in response to stress, and association of leaf and stem anthocyanin content with major stress tolerance traits. We suggest that anthocyanin accumulation can be considered as a marker of stress tolerance in tomato, and that LA-1996 can be considered for cultivation in salinity- or drought-affected areas.",
publisher = "Elsevier GmbH",
journal = "Journal of Plant Physiology",
title = "The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought",
number = "271",
doi = "10.1016/j.jplph.2022.153662",
pages = "153662"
}

Napar, W. P. F., Kaleri, A. R., Ahmed, A., Nabi, F., Sajid, S., Ćosić, T., Yao, Y., Liu, J., Raspor, M.,& Gao, Y.. (2022). The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought. in Journal of Plant Physiology
Elsevier GmbH.(271), 153662.
https://doi.org/10.1016/j.jplph.2022.153662

Napar WPF, Kaleri AR, Ahmed A, Nabi F, Sajid S, Ćosić T, Yao Y, Liu J, Raspor M, Gao Y. The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought. in Journal of Plant Physiology. 2022;(271):153662.
doi:10.1016/j.jplph.2022.153662 .

Napar, Wado Photo Faqir, Kaleri, Abdul Rasheed, Ahmed, Awais, Nabi, Farhan, Sajid, Sumbal, Ćosić, Tatjana, Yao, Yinan, Liu, Jikai, Raspor, Martin, Gao, Yongfeng, "The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought" in Journal of Plant Physiology, no. 271 (2022):153662,
https://doi.org/10.1016/j.jplph.2022.153662 . .

TY  - CHAP
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5094
AB  - De novo shoot organogenesis (DNSO) is a widely used procedure for obtaining regenerated plant shoots for biotechnological, industrial and conservation purposes. Particular morphogenic events leading to shoot regeneration are induced by plant hormones auxin and cytokinin that are exogenously supplemented to the regenerating explants through the nutrient media. It was shown, in Arabidopsis and in other plant species, that the early stages of DNSO are crucially dependent on auxin signaling and include the development of pluripotent primordia that are developmentally identical to lateral root primordia, whereas in the later stages, these primordia acquire the shoot identity and develop a shoot apical meristem (SAM) in a process that is governed mostly by cytokinin, but is also influenced by auxin signaling. In this chapter, we discuss the current state of knowledge about the particular components of auxin and cytokinin signaling that are specifically involved in the phytohormonal regulation of DNSO. Throughout DNSO, the auxin signaling multicellular domains are generated through the action of auxin influx and efflux carriers, whereas AUXIN RESPONSE FACTORs (ARFs), that are modulated by the Aux/IAA repressors, regulate gene expression during various stages of DNSO. Conversely, the cytokinin signals relevant for DNSO are perceived overwhelmingly through the receptor ARABIDOPSIS HISTIDINE KINASE4 (AHK4) and effected through the type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), whereas type-A ARRs act as attenuators of the cytokinin signals. Throughout DNSO, auxin and cytokinin signals enter mutual crosstalk, and crosstalk with other phytohormones, sugars and other developmental signals, for a complex and fine-tuned regulation of shoot regeneration.
PB  - Cham: Springer
T2  - Auxins, Cytokinins and Gibberellins Signaling in Plants
T1  - The Role of Auxin and Cytokinin Signaling Components in de novo Shoot Organogenesis
DO  - 10.1007/978-3-031-05427-3_3
SP  - 47
EP  - 75
ER  - 

@inbook{
author = "Ćosić, Tatjana and Raspor, Martin",
year = "2022",
abstract = "De novo shoot organogenesis (DNSO) is a widely used procedure for obtaining regenerated plant shoots for biotechnological, industrial and conservation purposes. Particular morphogenic events leading to shoot regeneration are induced by plant hormones auxin and cytokinin that are exogenously supplemented to the regenerating explants through the nutrient media. It was shown, in Arabidopsis and in other plant species, that the early stages of DNSO are crucially dependent on auxin signaling and include the development of pluripotent primordia that are developmentally identical to lateral root primordia, whereas in the later stages, these primordia acquire the shoot identity and develop a shoot apical meristem (SAM) in a process that is governed mostly by cytokinin, but is also influenced by auxin signaling. In this chapter, we discuss the current state of knowledge about the particular components of auxin and cytokinin signaling that are specifically involved in the phytohormonal regulation of DNSO. Throughout DNSO, the auxin signaling multicellular domains are generated through the action of auxin influx and efflux carriers, whereas AUXIN RESPONSE FACTORs (ARFs), that are modulated by the Aux/IAA repressors, regulate gene expression during various stages of DNSO. Conversely, the cytokinin signals relevant for DNSO are perceived overwhelmingly through the receptor ARABIDOPSIS HISTIDINE KINASE4 (AHK4) and effected through the type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), whereas type-A ARRs act as attenuators of the cytokinin signals. Throughout DNSO, auxin and cytokinin signals enter mutual crosstalk, and crosstalk with other phytohormones, sugars and other developmental signals, for a complex and fine-tuned regulation of shoot regeneration.",
publisher = "Cham: Springer",
journal = "Auxins, Cytokinins and Gibberellins Signaling in Plants",
booktitle = "The Role of Auxin and Cytokinin Signaling Components in de novo Shoot Organogenesis",
doi = "10.1007/978-3-031-05427-3_3",
pages = "47-75"
}

Ćosić, T.,& Raspor, M.. (2022). The Role of Auxin and Cytokinin Signaling Components in de novo Shoot Organogenesis. in Auxins, Cytokinins and Gibberellins Signaling in Plants
Cham: Springer., 47-75.
https://doi.org/10.1007/978-3-031-05427-3_3

Ćosić T, Raspor M. The Role of Auxin and Cytokinin Signaling Components in de novo Shoot Organogenesis. in Auxins, Cytokinins and Gibberellins Signaling in Plants. 2022;:47-75.
doi:10.1007/978-3-031-05427-3_3 .

Ćosić, Tatjana, Raspor, Martin, "The Role of Auxin and Cytokinin Signaling Components in de novo Shoot Organogenesis" in Auxins, Cytokinins and Gibberellins Signaling in Plants (2022):47-75,
https://doi.org/10.1007/978-3-031-05427-3_3 . .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Stanković-Jeremić, Jovana
AU  - Maksimović, Vuk
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - internal-pdf://Stupar et al. - 2021 - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf St.pdf
UR  - https://www.mdpi.com/2223-7747/10/1/172
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4147
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI AG
T2  - Plants
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
SP  - 172
ER  - 

@article{
author = "Stupar, Sofija and Dragićević, Milan and Tešević, Vele and Stanković-Jeremić, Jovana and Maksimović, Vuk and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI AG",
journal = "Plants",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172",
pages = "172"
}

Stupar, S., Dragićević, M., Tešević, V., Stanković-Jeremić, J., Maksimović, V., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants
MDPI AG., 10(1), 172.
https://doi.org/10.3390/plants10010172

Stupar S, Dragićević M, Tešević V, Stanković-Jeremić J, Maksimović V, Ćosić T, Devrnja N, Tubić L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants. 2021;10(1):172.
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragićević, Milan, Tešević, Vele, Stanković-Jeremić, Jovana, Maksimović, Vuk, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants, 10, no. 1 (2021):172,
https://doi.org/10.3390/plants10010172 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Marković, Marija
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2021
UR  - https://www.nature.com/articles/s41598-021-85932-w
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4194
AB  - Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.
PB  - Springer Science and Business Media LLC
T2  - Scientific Reports
T1  - Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-85932-w
SP  - 6494
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Václav and Savić, Jelena and Raspor, Martin and Marković, Marija and Dobrev, Petre I. and Ninković, Slavica",
year = "2021",
abstract = "Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.",
publisher = "Springer Science and Business Media LLC",
journal = "Scientific Reports",
title = "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-85932-w",
pages = "6494"
}

Ćosić, T., Motyka, V., Savić, J., Raspor, M., Marković, M., Dobrev, P. I.,& Ninković, S.. (2021). Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports
Springer Science and Business Media LLC., 11(1), 6494.
https://doi.org/10.1038/s41598-021-85932-w

Ćosić T, Motyka V, Savić J, Raspor M, Marković M, Dobrev PI, Ninković S. Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports. 2021;11(1):6494.
doi:10.1038/s41598-021-85932-w .

Ćosić, Tatjana, Motyka, Václav, Savić, Jelena, Raspor, Martin, Marković, Marija, Dobrev, Petre I., Ninković, Slavica, "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi" in Scientific Reports, 11, no. 1 (2021):6494,
https://doi.org/10.1038/s41598-021-85932-w . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Kaleri, Abdul Rasheed
AU  - Ninković, Slavica
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ćosić, Tatjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4303
AB  - De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs
IS  - 16
VL  - 22
DO  - 10.3390/ijms22168554
SP  - 8554
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Kaleri, Abdul Rasheed and Ninković, Slavica and Tubić, Ljiljana and Cingel, Aleksandar and Ćosić, Tatjana",
year = "2021",
abstract = "De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs",
number = "16",
volume = "22",
doi = "10.3390/ijms22168554",
pages = "8554"
}

Raspor, M., Motyka, V., Kaleri, A. R., Ninković, S., Tubić, L., Cingel, A.,& Ćosić, T.. (2021). Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences
Basel: MDPI., 22(16), 8554.
https://doi.org/10.3390/ijms22168554

Raspor M, Motyka V, Kaleri AR, Ninković S, Tubić L, Cingel A, Ćosić T. Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences. 2021;22(16):8554.
doi:10.3390/ijms22168554 .

Raspor, Martin, Motyka, Václav, Kaleri, Abdul Rasheed, Ninković, Slavica, Tubić, Ljiljana, Cingel, Aleksandar, Ćosić, Tatjana, "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs" in International Journal of Molecular Sciences, 22, no. 16 (2021):8554,
https://doi.org/10.3390/ijms22168554 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Cingel, Aleksandar
AU  - Ghalawnji, Nabil
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3816
AB  - Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of
the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators
(PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were
studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18±2°C on half-strength
MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose
and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA),
gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all
treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a
distinctive manner, with a variety of morphological traits being altered in comparison to matching
controls.
PB  - Serbian Biological Socitey
T2  - Archives of Biological Sciences
T1  - Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro
IS  - 3
VL  - 72
DO  - 10.2298/ABS200622029C
SP  - 349
EP  - 357
ER  - 

@article{
author = "Ćosić, Tatjana and Savić, Jelena and Raspor, Martin and Cingel, Aleksandar and Ghalawnji, Nabil and Vinterhalter, Branka and Ninković, Slavica",
year = "2020",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of
the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators
(PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were
studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18±2°C on half-strength
MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose
and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA),
gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all
treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a
distinctive manner, with a variety of morphological traits being altered in comparison to matching
controls.",
publisher = "Serbian Biological Socitey",
journal = "Archives of Biological Sciences",
title = "Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro",
number = "3",
volume = "72",
doi = "10.2298/ABS200622029C",
pages = "349-357"
}

Ćosić, T., Savić, J., Raspor, M., Cingel, A., Ghalawnji, N., Vinterhalter, B.,& Ninković, S.. (2020). Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. in Archives of Biological Sciences
Serbian Biological Socitey., 72(3), 349-357.
https://doi.org/10.2298/ABS200622029C

Ćosić T, Savić J, Raspor M, Cingel A, Ghalawnji N, Vinterhalter B, Ninković S. Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. in Archives of Biological Sciences. 2020;72(3):349-357.
doi:10.2298/ABS200622029C .

Ćosić, Tatjana, Savić, Jelena, Raspor, Martin, Cingel, Aleksandar, Ghalawnji, Nabil, Vinterhalter, Branka, Ninković, Slavica, "Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro" in Archives of Biological Sciences, 72, no. 3 (2020):349-357,
https://doi.org/10.2298/ABS200622029C . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Dobrev, Petre I.
AU  - Malbeck, Jiří
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2020
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7044434
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3623
AB  - A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.
T2  - Scientific Reports
T1  - Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-60412-9
SP  - 3437
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Dobrev, Petre I. and Malbeck, Jiří and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Tadić, Vojin and Dragićević, Ivana",
year = "2020",
abstract = "A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.",
journal = "Scientific Reports",
title = "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-60412-9",
pages = "3437"
}

Raspor, M., Motyka, V., Ninković, S., Dobrev, P. I., Malbeck, J., Ćosić, T., Cingel, A., Savić, J., Tadić, V.,& Dragićević, I.. (2020). Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports, 10(1), 3437.
https://doi.org/10.1038/s41598-020-60412-9

Raspor M, Motyka V, Ninković S, Dobrev PI, Malbeck J, Ćosić T, Cingel A, Savić J, Tadić V, Dragićević I. Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports. 2020;10(1):3437.
doi:10.1038/s41598-020-60412-9 .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Dobrev, Petre I., Malbeck, Jiří, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Tadić, Vojin, Dragićević, Ivana, "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics." in Scientific Reports, 10, no. 1 (2020):3437,
https://doi.org/10.1038/s41598-020-60412-9 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Matekalo, Dragana
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161718304140?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3213
AB  - Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species.
T2  - Journal of Plant Physiology
T1  - Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.
VL  - 232
DO  - 10.1016/j.jplph.2018.11.004
SP  - 257
EP  - 269
ER  - 

@article{
author = "Ćosić, Tatjana and Raspor, Martin and Savić, Jelena and Cingel, Aleksandar and Matekalo, Dragana and Zdravković-Korać, Snežana and Ninković, Slavica",
year = "2019",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species.",
journal = "Journal of Plant Physiology",
title = "Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.",
volume = "232",
doi = "10.1016/j.jplph.2018.11.004",
pages = "257-269"
}

Ćosić, T., Raspor, M., Savić, J., Cingel, A., Matekalo, D., Zdravković-Korać, S.,& Ninković, S.. (2019). Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.. in Journal of Plant Physiology, 232, 257-269.
https://doi.org/10.1016/j.jplph.2018.11.004

Ćosić T, Raspor M, Savić J, Cingel A, Matekalo D, Zdravković-Korać S, Ninković S. Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.. in Journal of Plant Physiology. 2019;232:257-269.
doi:10.1016/j.jplph.2018.11.004 .

Ćosić, Tatjana, Raspor, Martin, Savić, Jelena, Cingel, Aleksandar, Matekalo, Dragana, Zdravković-Korać, Snežana, Ninković, Slavica, "Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi." in Journal of Plant Physiology, 232 (2019):257-269,
https://doi.org/10.1016/j.jplph.2018.11.004 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6129
AB  - Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.
PB  - Olomouc: Europian Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration
SP  - 131
EP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6129
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Vaclav and Savić, Jelena and Cingel, Aleksandar and Raspor, Martin and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2019",
abstract = "Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.",
publisher = "Olomouc: Europian Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration",
pages = "131-131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6129"
}

Ćosić, T., Motyka, V., Savić, J., Cingel, A., Raspor, M., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2019). Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
Olomouc: Europian Federation of Biotechnology., 131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129

Ćosić T, Motyka V, Savić J, Cingel A, Raspor M, Devrnja N, Dobrev PI, Ninković S. Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

Ćosić, Tatjana, Motyka, Vaclav, Savić, Jelena, Cingel, Aleksandar, Raspor, Martin, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):131-131,
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

TY  - CONF
AU  - Stupar, Sofija
AU  - Avramović, Miloš
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5593
AB  - Potato fields pest protection is still predominantly based on synthetic pesticides, expensive in terms of monetary investment and proved to be harmful to human health and the environment. Alternatively, flower strips of French marigold (Tagetes patula L.) appearing as potent pest repellents in potato fields, offer well-adopted practice in organic farming. Up to now, the effect of French marigoldon potato metabolism, physiology, and yield has not been studied in detail. Thus, a microarray transcriptome analysis was performed to study the effect of French marigold essential oil (EO) on laboratory-grown potato, with the focus on starch metabolism pathway. After 8h of exposure to EO, 3840 potato probes were differentially expressed (fold change ≥2) compared to control plants, of which 1744 were upregulated and 2096 downregulated. We selected a set of putative genes involved in the starch metabolism pathway in plastids, and validated microarray patterns using RT-qPCR method. The expression of selected genes was analyzed in plants exposed to EO for 4, 8 and 12h . While the expression of genes involved in starch biosynthesis was at similar levels as in non-treated plants, the most of the genes responsible for gradual degradation of starch displayed significantly elevated expression with the highest levels detected after 8 and 12h of exposure. Our results provide a comprehensive sequence resource for studying the potato-EO interactions, revealing induced alternations in potato starch metabolism and suggesting modified regulatory mechanism in potato affected by EO.
PB  - European Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5593
ER  - 

@conference{
author = "Stupar, Sofija and Avramović, Miloš and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2019",
abstract = "Potato fields pest protection is still predominantly based on synthetic pesticides, expensive in terms of monetary investment and proved to be harmful to human health and the environment. Alternatively, flower strips of French marigold (Tagetes patula L.) appearing as potent pest repellents in potato fields, offer well-adopted practice in organic farming. Up to now, the effect of French marigoldon potato metabolism, physiology, and yield has not been studied in detail. Thus, a microarray transcriptome analysis was performed to study the effect of French marigold essential oil (EO) on laboratory-grown potato, with the focus on starch metabolism pathway. After 8h of exposure to EO, 3840 potato probes were differentially expressed (fold change ≥2) compared to control plants, of which 1744 were upregulated and 2096 downregulated. We selected a set of putative genes involved in the starch metabolism pathway in plastids, and validated microarray patterns using RT-qPCR method. The expression of selected genes was analyzed in plants exposed to EO for 4, 8 and 12h . While the expression of genes involved in starch biosynthesis was at similar levels as in non-treated plants, the most of the genes responsible for gradual degradation of starch displayed significantly elevated expression with the highest levels detected after 8 and 12h of exposure. Our results provide a comprehensive sequence resource for studying the potato-EO interactions, revealing induced alternations in potato starch metabolism and suggesting modified regulatory mechanism in potato affected by EO.",
publisher = "European Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5593"
}

Stupar, S., Avramović, M., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2019). Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
European Federation of Biotechnology., 129.
https://hdl.handle.net/21.15107/rcub_ibiss_5593

Stupar S, Avramović M, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:129.
https://hdl.handle.net/21.15107/rcub_ibiss_5593 .

Stupar, Sofija, Avramović, Miloš, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):129,
https://hdl.handle.net/21.15107/rcub_ibiss_5593 .