TY  - JOUR
AU  - Jakovljević, Danijel
AU  - Nikolić, Milan
AU  - Jovanović, Vesna
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić-Kokić, Aleksandra
AU  - Novaković, Emilija
AU  - Miljević, Čedo
AU  - Milovanovć, Maja
AU  - Blagojević, Duško
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6510
AB  - Background: Epilepsy is a chronic brain disease affecting millions of people worldwide,
but little is known about the impact of anti-seizure medications on redox homeostasis. Methods:
This study aimed to compare the effects of the long-term use of oral anti-seizure medications in
monotherapy (lamotrigine, carbamazepine, and valproate) on antioxidant enzymes: superoxide dismutase,
catalase, glutathione peroxidase, glutathione reductase, haemoglobin, and methaemoglobin
content in erythrocytes, and concentrations of total proteins and thiols, nitrites, lipid peroxides and
total glutathione in the plasma of epilepsy patients and drug-naïve patients. Results: The results
showed that lamotrigine therapy led to lower superoxide dismutase activity (p < 0.005) and lower
concentrations of total thiols (p < 0.01) and lipid peroxides (p < 0.01) compared to controls. On the
other hand, therapy with carbamazepine increased nitrite levels (p < 0.01) but reduced superoxide
dismutase activity (p < 0.005). In the valproate group, only a decrease in catalase activity was observed
(p < 0.005). Canonical discriminant analysis showed that the composition of antioxidant enzymes in
erythrocytes was different for both the lamotrigine and carbamazepine groups, while the controls
were separated from all others. Conclusions: Monotherapy with anti-seizure medications discretely
alters redox homeostasis, followed by distinct relationships between antioxidant components.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood
IS  - 1
VL  - 17
DO  - 10.3390/ph17010130
SP  - 130
ER  - 

@article{
author = "Jakovljević, Danijel and Nikolić, Milan and Jovanović, Vesna and Vidonja Uzelac, Teodora and Nikolić-Kokić, Aleksandra and Novaković, Emilija and Miljević, Čedo and Milovanovć, Maja and Blagojević, Duško",
year = "2024",
abstract = "Background: Epilepsy is a chronic brain disease affecting millions of people worldwide,
but little is known about the impact of anti-seizure medications on redox homeostasis. Methods:
This study aimed to compare the effects of the long-term use of oral anti-seizure medications in
monotherapy (lamotrigine, carbamazepine, and valproate) on antioxidant enzymes: superoxide dismutase,
catalase, glutathione peroxidase, glutathione reductase, haemoglobin, and methaemoglobin
content in erythrocytes, and concentrations of total proteins and thiols, nitrites, lipid peroxides and
total glutathione in the plasma of epilepsy patients and drug-naïve patients. Results: The results
showed that lamotrigine therapy led to lower superoxide dismutase activity (p < 0.005) and lower
concentrations of total thiols (p < 0.01) and lipid peroxides (p < 0.01) compared to controls. On the
other hand, therapy with carbamazepine increased nitrite levels (p < 0.01) but reduced superoxide
dismutase activity (p < 0.005). In the valproate group, only a decrease in catalase activity was observed
(p < 0.005). Canonical discriminant analysis showed that the composition of antioxidant enzymes in
erythrocytes was different for both the lamotrigine and carbamazepine groups, while the controls
were separated from all others. Conclusions: Monotherapy with anti-seizure medications discretely
alters redox homeostasis, followed by distinct relationships between antioxidant components.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood",
number = "1",
volume = "17",
doi = "10.3390/ph17010130",
pages = "130"
}

Jakovljević, D., Nikolić, M., Jovanović, V., Vidonja Uzelac, T., Nikolić-Kokić, A., Novaković, E., Miljević, Č., Milovanovć, M.,& Blagojević, D.. (2024). Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood. in Pharmaceuticals
Basel: MDPI., 17(1), 130.
https://doi.org/10.3390/ph17010130

Jakovljević D, Nikolić M, Jovanović V, Vidonja Uzelac T, Nikolić-Kokić A, Novaković E, Miljević Č, Milovanovć M, Blagojević D. Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood. in Pharmaceuticals. 2024;17(1):130.
doi:10.3390/ph17010130 .

Jakovljević, Danijel, Nikolić, Milan, Jovanović, Vesna, Vidonja Uzelac, Teodora, Nikolić-Kokić, Aleksandra, Novaković, Emilija, Miljević, Čedo, Milovanovć, Maja, Blagojević, Duško, "Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood" in Pharmaceuticals, 17, no. 1 (2024):130,
https://doi.org/10.3390/ph17010130 . .

TY  - JOUR
AU  - Vukčević, Marija
AU  - Šerović, Katarina
AU  - Despot, Mateja
AU  - Nikolić-Kokić, Aleksandra
AU  - Vujović, Aleksandra
AU  - Nikolić, Milan
AU  - Blagojević, Duško
AU  - Jovanović, Tanja
AU  - Despot, Dragana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6495
AB  - Background: Several vaccines against COVID-19 have been developed and licensed to
enhance the immune response against SARS-CoV-2. Similarly, previous infection with SARS-CoV-
2 has been shown to provide significant protection against severe infection and hospitalization.
Methods: We investigated the effect of three doses of the Sinopharm vaccine and SARS-CoV-2
infection on the specific immune response in 103 volunteers, measuring neutralizing antibodies, anti-
S1 IgG, anti-RBD IgM, anti-N IgM, anti-N IgG antibodies, and INF γ. Results: Our results showed
that the presence of cardiovascular diseases increased the level of anti-N-IgG antibodies, while
endocrinological diseases decreased the level of neutralizing antibodies and anti-N IgG antibodies,
suggesting that these diseases alter the effect of vaccine-induced immunity. In addition, there was a
significant decrease in anti-S1 IgG levels at 6 months and in anti-N IgG levels 18 months post-infection,
while neutralizing antibodies and INF γ levels were constant at 3, 6, and 18 months post-infection.
Conclusions: Our results confirm the emergence of hybrid immunity, which is the strongest and
most durable compared to natural immunity or vaccine-induced immunity. Significant positive
correlations were found between humoral and cellular immunity markers: neutralizing antibodies,
anti-S1 IgG and anti-N IgG antibodies, and INF γ, indicating a unique coordinated response specific
to COVID-19.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Humoral and Cellular Immune Response after Three Doses of Sinopharm [Vero Cell]-Inactivated COVID-19 Vaccine in Combination with SARS-CoV-2 Infection Leads to Hybrid Immunity
IS  - 1
VL  - 17
DO  - 10.3390/ph17010122
SP  - 122
ER  - 

@article{
author = "Vukčević, Marija and Šerović, Katarina and Despot, Mateja and Nikolić-Kokić, Aleksandra and Vujović, Aleksandra and Nikolić, Milan and Blagojević, Duško and Jovanović, Tanja and Despot, Dragana",
year = "2024",
abstract = "Background: Several vaccines against COVID-19 have been developed and licensed to
enhance the immune response against SARS-CoV-2. Similarly, previous infection with SARS-CoV-
2 has been shown to provide significant protection against severe infection and hospitalization.
Methods: We investigated the effect of three doses of the Sinopharm vaccine and SARS-CoV-2
infection on the specific immune response in 103 volunteers, measuring neutralizing antibodies, anti-
S1 IgG, anti-RBD IgM, anti-N IgM, anti-N IgG antibodies, and INF γ. Results: Our results showed
that the presence of cardiovascular diseases increased the level of anti-N-IgG antibodies, while
endocrinological diseases decreased the level of neutralizing antibodies and anti-N IgG antibodies,
suggesting that these diseases alter the effect of vaccine-induced immunity. In addition, there was a
significant decrease in anti-S1 IgG levels at 6 months and in anti-N IgG levels 18 months post-infection,
while neutralizing antibodies and INF γ levels were constant at 3, 6, and 18 months post-infection.
Conclusions: Our results confirm the emergence of hybrid immunity, which is the strongest and
most durable compared to natural immunity or vaccine-induced immunity. Significant positive
correlations were found between humoral and cellular immunity markers: neutralizing antibodies,
anti-S1 IgG and anti-N IgG antibodies, and INF γ, indicating a unique coordinated response specific
to COVID-19.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Humoral and Cellular Immune Response after Three Doses of Sinopharm [Vero Cell]-Inactivated COVID-19 Vaccine in Combination with SARS-CoV-2 Infection Leads to Hybrid Immunity",
number = "1",
volume = "17",
doi = "10.3390/ph17010122",
pages = "122"
}

Vukčević, M., Šerović, K., Despot, M., Nikolić-Kokić, A., Vujović, A., Nikolić, M., Blagojević, D., Jovanović, T.,& Despot, D.. (2024). Humoral and Cellular Immune Response after Three Doses of Sinopharm [Vero Cell]-Inactivated COVID-19 Vaccine in Combination with SARS-CoV-2 Infection Leads to Hybrid Immunity. in Pharmaceuticals
Basel: MDPI., 17(1), 122.
https://doi.org/10.3390/ph17010122

Vukčević M, Šerović K, Despot M, Nikolić-Kokić A, Vujović A, Nikolić M, Blagojević D, Jovanović T, Despot D. Humoral and Cellular Immune Response after Three Doses of Sinopharm [Vero Cell]-Inactivated COVID-19 Vaccine in Combination with SARS-CoV-2 Infection Leads to Hybrid Immunity. in Pharmaceuticals. 2024;17(1):122.
doi:10.3390/ph17010122 .

Vukčević, Marija, Šerović, Katarina, Despot, Mateja, Nikolić-Kokić, Aleksandra, Vujović, Aleksandra, Nikolić, Milan, Blagojević, Duško, Jovanović, Tanja, Despot, Dragana, "Humoral and Cellular Immune Response after Three Doses of Sinopharm [Vero Cell]-Inactivated COVID-19 Vaccine in Combination with SARS-CoV-2 Infection Leads to Hybrid Immunity" in Pharmaceuticals, 17, no. 1 (2024):122,
https://doi.org/10.3390/ph17010122 . .

TY  - JOUR
AU  - Vukčević, Marija
AU  - Nikolić-Kokić, Aleksandra
AU  - Aleksić, Ivan
AU  - Todorović, Sanja
AU  - Oreščanin-Dušić, Zorana
AU  - Blagojević, Duško
AU  - Despot, Dragana
PY  - 2023
UR  - https://academic.oup.com/jee/advance-article/doi/10.1093/jee/toac190/6927190
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5386
AB  - Controlling the number of ticks as carriers of infectious diseases is very important. The process is sometimes compromised by activating the protective mechanisms of the tick itself. Glutathione-S-transferases activity (GSTs) was the subject of our investigation of tick abundance after pyrethroid treatment. We determined GSTs activity in ticks collected from six locations in Belgrade before and after pyrethroid treatment and correlated it with the number of ticks in the locations. The results showed that tick abundance correlated with GSTs activity. On the other hand, treatment efficiency was location-dependent, being similar in each particular location in both April (spring) and October (autumn). Our results suggest that GSTs activity reflects the influence of both present local allelochemicals from different environmental seasonal vegetation and applied pyrethroid. We can conclude that by evaluating GSTs activity in ticks from particular locations as well as during the treatment with acaricides tick removal practice could be improved.
T2  - Journal of Economic Entomology
T1  - Evaluation of Tick Abundance and Pyrethroid Resistance Via Determination of Glutathione-S-Transferases Activity
IS  - 1
VL  - 116
DO  - 10.1093/jee/toac190
SP  - 233
EP  - 239
ER  - 

@article{
author = "Vukčević, Marija and Nikolić-Kokić, Aleksandra and Aleksić, Ivan and Todorović, Sanja and Oreščanin-Dušić, Zorana and Blagojević, Duško and Despot, Dragana",
year = "2023",
abstract = "Controlling the number of ticks as carriers of infectious diseases is very important. The process is sometimes compromised by activating the protective mechanisms of the tick itself. Glutathione-S-transferases activity (GSTs) was the subject of our investigation of tick abundance after pyrethroid treatment. We determined GSTs activity in ticks collected from six locations in Belgrade before and after pyrethroid treatment and correlated it with the number of ticks in the locations. The results showed that tick abundance correlated with GSTs activity. On the other hand, treatment efficiency was location-dependent, being similar in each particular location in both April (spring) and October (autumn). Our results suggest that GSTs activity reflects the influence of both present local allelochemicals from different environmental seasonal vegetation and applied pyrethroid. We can conclude that by evaluating GSTs activity in ticks from particular locations as well as during the treatment with acaricides tick removal practice could be improved.",
journal = "Journal of Economic Entomology",
title = "Evaluation of Tick Abundance and Pyrethroid Resistance Via Determination of Glutathione-S-Transferases Activity",
number = "1",
volume = "116",
doi = "10.1093/jee/toac190",
pages = "233-239"
}

Vukčević, M., Nikolić-Kokić, A., Aleksić, I., Todorović, S., Oreščanin-Dušić, Z., Blagojević, D.,& Despot, D.. (2023). Evaluation of Tick Abundance and Pyrethroid Resistance Via Determination of Glutathione-S-Transferases Activity. in Journal of Economic Entomology, 116(1), 233-239.
https://doi.org/10.1093/jee/toac190

Vukčević M, Nikolić-Kokić A, Aleksić I, Todorović S, Oreščanin-Dušić Z, Blagojević D, Despot D. Evaluation of Tick Abundance and Pyrethroid Resistance Via Determination of Glutathione-S-Transferases Activity. in Journal of Economic Entomology. 2023;116(1):233-239.
doi:10.1093/jee/toac190 .

Vukčević, Marija, Nikolić-Kokić, Aleksandra, Aleksić, Ivan, Todorović, Sanja, Oreščanin-Dušić, Zorana, Blagojević, Duško, Despot, Dragana, "Evaluation of Tick Abundance and Pyrethroid Resistance Via Determination of Glutathione-S-Transferases Activity" in Journal of Economic Entomology, 116, no. 1 (2023):233-239,
https://doi.org/10.1093/jee/toac190 . .

TY  - CONF
AU  - Grahovac, Tanja
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić-Kokić, Aleksandra
AU  - Tatalović, Nikola
AU  - Oreščanin-Dušić, Zorana
AU  - Blagojević, Duško
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5849
AB  - Normal redox status of the cell is maintained by a number of enzymes, such as superoxide dismutase, catalase, glutathione peroxidase and low molecular weight antioxidants. One of the component that determining the redox status of cells is the tripeptide glutathione. Maintaining high levels of reduced glutathione enhances antioxidant defense. The aim of this study was to examine whether, to what extent and in what way changes in physiological processes occurred in conditions when cellular redox homeostasis is in imbalance. The experiments were performed on isolated ileum of male Wistar rats, electrically stimulated and treated with cumulative doses of reduced or oxidized glutathione. The activity of antioxidant enzymes (catalase, glutathione reductase, glutathione peroxidase, copper–zinc superoxide dismutase and manganese superoxide dismutase) was determined in treated ileum.  Glutathione in both forms decreased amplitude of ileum contractions. Cumulative doses of oxidized glutathione led to higher glutathione reductase activity while the addition of reduced glutathione increased activity of glutathione peroxidase. The results showed that maintenance of cellular redox homeostasis have influence to physiological processes.
PB  - Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina -ABMBBIH; 2023 May 18-20; Sarajevo, Bosnia and Herzegovina
T1  - Reduced and oxidized glutathione affects smooth muscle contractility and anti-oxidant enzymes activity in isolated ileum of male rat
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5849
ER  - 

@conference{
author = "Grahovac, Tanja and Vidonja Uzelac, Teodora and Nikolić-Kokić, Aleksandra and Tatalović, Nikola and Oreščanin-Dušić, Zorana and Blagojević, Duško",
year = "2023",
abstract = "Normal redox status of the cell is maintained by a number of enzymes, such as superoxide dismutase, catalase, glutathione peroxidase and low molecular weight antioxidants. One of the component that determining the redox status of cells is the tripeptide glutathione. Maintaining high levels of reduced glutathione enhances antioxidant defense. The aim of this study was to examine whether, to what extent and in what way changes in physiological processes occurred in conditions when cellular redox homeostasis is in imbalance. The experiments were performed on isolated ileum of male Wistar rats, electrically stimulated and treated with cumulative doses of reduced or oxidized glutathione. The activity of antioxidant enzymes (catalase, glutathione reductase, glutathione peroxidase, copper–zinc superoxide dismutase and manganese superoxide dismutase) was determined in treated ileum.  Glutathione in both forms decreased amplitude of ileum contractions. Cumulative doses of oxidized glutathione led to higher glutathione reductase activity while the addition of reduced glutathione increased activity of glutathione peroxidase. The results showed that maintenance of cellular redox homeostasis have influence to physiological processes.",
publisher = "Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina -ABMBBIH; 2023 May 18-20; Sarajevo, Bosnia and Herzegovina",
title = "Reduced and oxidized glutathione affects smooth muscle contractility and anti-oxidant enzymes activity in isolated ileum of male rat",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5849"
}

Grahovac, T., Vidonja Uzelac, T., Nikolić-Kokić, A., Tatalović, N., Oreščanin-Dušić, Z.,& Blagojević, D.. (2023). Reduced and oxidized glutathione affects smooth muscle contractility and anti-oxidant enzymes activity in isolated ileum of male rat. in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina -ABMBBIH; 2023 May 18-20; Sarajevo, Bosnia and Herzegovina
Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 42.
https://hdl.handle.net/21.15107/rcub_ibiss_5849

Grahovac T, Vidonja Uzelac T, Nikolić-Kokić A, Tatalović N, Oreščanin-Dušić Z, Blagojević D. Reduced and oxidized glutathione affects smooth muscle contractility and anti-oxidant enzymes activity in isolated ileum of male rat. in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina -ABMBBIH; 2023 May 18-20; Sarajevo, Bosnia and Herzegovina. 2023;:42.
https://hdl.handle.net/21.15107/rcub_ibiss_5849 .

Grahovac, Tanja, Vidonja Uzelac, Teodora, Nikolić-Kokić, Aleksandra, Tatalović, Nikola, Oreščanin-Dušić, Zorana, Blagojević, Duško, "Reduced and oxidized glutathione affects smooth muscle contractility and anti-oxidant enzymes activity in isolated ileum of male rat" in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina -ABMBBIH; 2023 May 18-20; Sarajevo, Bosnia and Herzegovina (2023):42,
https://hdl.handle.net/21.15107/rcub_ibiss_5849 .

TY  - JOUR
AU  - Savić, Bojana
AU  - Brkljačić, Jelena
AU  - Glumac, Sofija
AU  - Šarenac, Olivera
AU  - Murphy, David
AU  - Blagojević, Duško
AU  - Japundžić-Žigon, Nina
AU  - Oreščanin-Dušić, Zorana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5759
AB  - Hypertension and its complications are a leading cause of death in the human
population. Several factors can contribute to development of hypertension, such
as genetic predisposition, high salt intake and environmental stressors, underlying
oxidative stress as one of its key trademarks. We studied the effects of increased salt
intake and chronic stress on blood pressure parameters and the activity and protein
levels of antioxidant enzymes in the heart and aorta of borderline hypertensive rats
(BHRs) with genetic susceptibility to hypertension. All animals were randomized into
four groups: (1) Wistar rats kept in baseline conditions; (2) BHRs kept in baseline
conditions; (3) BHRs drinking 0.9% saline solution; and (4) BHRs drinking 0.9% saline
solution and exposed to repeated heterotypic stress. The BHRs exhibited significantly
higher blood pressure, mitochondrial superoxide dismutase (SOD2) and catalase (CAT)
protein levels and lower glutathione peroxidase (GPx) and glutathione reductase (GR)
activities in the aorta, followed by lower CAT and GPx protein levels and higher CAT
and GR activities in the heart, compared with normotensive Wistar rats. In the BHR
aorta, high salt intake elevated CAT and GPx activities, and when combined with stress
it increased GPx and GR activities. In BHR hearts, high salt intake provoked lower CAT
activity. Adding repeated stress to salt treatment further decreased CAT activity, in
addition to Cu2+–Zn2+ superoxide dismutase (SOD1) and GR activities. The protein
level of CAT was lower, whereas SOD2 and GPx increased. Overall, our results suggest
that BHR hearts are better adapted to oxidative pressure, compared with the aorta,
when exposed to salt and stress.
PB  - Hoboken: Wiley
T2  - Experimental Physiology
T1  - Effects of salt and stress on blood pressure parameters and antioxidant enzyme function in the heart and aorta of borderline hypertensive rats
IS  - 7
VL  - 108
DO  - 10.1113/EP090714
SP  - 946
EP  - 960
ER  - 

@article{
author = "Savić, Bojana and Brkljačić, Jelena and Glumac, Sofija and Šarenac, Olivera and Murphy, David and Blagojević, Duško and Japundžić-Žigon, Nina and Oreščanin-Dušić, Zorana",
year = "2023",
abstract = "Hypertension and its complications are a leading cause of death in the human
population. Several factors can contribute to development of hypertension, such
as genetic predisposition, high salt intake and environmental stressors, underlying
oxidative stress as one of its key trademarks. We studied the effects of increased salt
intake and chronic stress on blood pressure parameters and the activity and protein
levels of antioxidant enzymes in the heart and aorta of borderline hypertensive rats
(BHRs) with genetic susceptibility to hypertension. All animals were randomized into
four groups: (1) Wistar rats kept in baseline conditions; (2) BHRs kept in baseline
conditions; (3) BHRs drinking 0.9% saline solution; and (4) BHRs drinking 0.9% saline
solution and exposed to repeated heterotypic stress. The BHRs exhibited significantly
higher blood pressure, mitochondrial superoxide dismutase (SOD2) and catalase (CAT)
protein levels and lower glutathione peroxidase (GPx) and glutathione reductase (GR)
activities in the aorta, followed by lower CAT and GPx protein levels and higher CAT
and GR activities in the heart, compared with normotensive Wistar rats. In the BHR
aorta, high salt intake elevated CAT and GPx activities, and when combined with stress
it increased GPx and GR activities. In BHR hearts, high salt intake provoked lower CAT
activity. Adding repeated stress to salt treatment further decreased CAT activity, in
addition to Cu2+–Zn2+ superoxide dismutase (SOD1) and GR activities. The protein
level of CAT was lower, whereas SOD2 and GPx increased. Overall, our results suggest
that BHR hearts are better adapted to oxidative pressure, compared with the aorta,
when exposed to salt and stress.",
publisher = "Hoboken: Wiley",
journal = "Experimental Physiology",
title = "Effects of salt and stress on blood pressure parameters and antioxidant enzyme function in the heart and aorta of borderline hypertensive rats",
number = "7",
volume = "108",
doi = "10.1113/EP090714",
pages = "946-960"
}

Savić, B., Brkljačić, J., Glumac, S., Šarenac, O., Murphy, D., Blagojević, D., Japundžić-Žigon, N.,& Oreščanin-Dušić, Z.. (2023). Effects of salt and stress on blood pressure parameters and antioxidant enzyme function in the heart and aorta of borderline hypertensive rats. in Experimental Physiology
Hoboken: Wiley., 108(7), 946-960.
https://doi.org/10.1113/EP090714

Savić B, Brkljačić J, Glumac S, Šarenac O, Murphy D, Blagojević D, Japundžić-Žigon N, Oreščanin-Dušić Z. Effects of salt and stress on blood pressure parameters and antioxidant enzyme function in the heart and aorta of borderline hypertensive rats. in Experimental Physiology. 2023;108(7):946-960.
doi:10.1113/EP090714 .

Savić, Bojana, Brkljačić, Jelena, Glumac, Sofija, Šarenac, Olivera, Murphy, David, Blagojević, Duško, Japundžić-Žigon, Nina, Oreščanin-Dušić, Zorana, "Effects of salt and stress on blood pressure parameters and antioxidant enzyme function in the heart and aorta of borderline hypertensive rats" in Experimental Physiology, 108, no. 7 (2023):946-960,
https://doi.org/10.1113/EP090714 . .

TY  - JOUR
AU  - Kulić, Milan
AU  - Drakul, Dragana
AU  - Sokolović, Dragana
AU  - Kordić-Bojinović, Jelena
AU  - Milovanović, Slobodan
AU  - Blagojević, Duško
PY  - 2023
UR  - http://www.acgpubs.org/journal/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5387
UR  - http://www.acgpubs.org/article/records-of-natural-products/in-press/satureja-montana-l-from-herzegovina-assessment-of-composition-antispasmodic-and-antidiarrheal-effects
AB  - Satureja montana L. (SM) has a long traditional use as a spice and a medicine for various gastrointestinal disorders, including painful spasms and diarrhea. Contrary to conventional drugs, administration of SM and its extracts are considered safe. Previous studies have shown that the essential oils (EOs) of SM from different areas are rich in monoterpenes, sesquiterpenes, diterpens, and phenolic compounds, including flavonoids, tannins, and acids with great composition variability. Determination of composition of EO from Herzegovinian SM done by gas chromatography-flame ionization detection and gas chromatography mass spectrometry (GC-FID and GC/MS, respectively) revealed carvacrol as a primary substance followed by γ-terpinene, p-cymene, and β-caryophyllene. Ex vivo spasmolitic activity caused by EO was evident in different types of isolated rat ileum function with the most potent effect on spontaneous activity followed by electrical field stimulation and KCl-and CaCl2-induced activity. SMEO produced in vivo antidiarreal activity on castor oil-induced diarrhea in young rats and showed the potential to cause a decrese water content in the feces of adult Wistar rats.This study indicates that effects of SM on the intestinum could be mediated through combination of Kv channel activation and Ca channel blockade, but additional mechanisms might be involved. The results of this study corroborate the traditional use of SM as antispasmodic, antidiarrheal, and antisecretory agents.
T2  - Records of Natural Products
T1  - Essential Oil of Satureja montana L. from Herzegovina: Assessment of Composition, Antispasmodic, and Antidiarrheal Effects
IS  - 3
VL  - 17
DO  - 10.25135/rnp.358.2207.2522
SP  - 536
EP  - 548
ER  - 

@article{
author = "Kulić, Milan and Drakul, Dragana and Sokolović, Dragana and Kordić-Bojinović, Jelena and Milovanović, Slobodan and Blagojević, Duško",
year = "2023",
abstract = "Satureja montana L. (SM) has a long traditional use as a spice and a medicine for various gastrointestinal disorders, including painful spasms and diarrhea. Contrary to conventional drugs, administration of SM and its extracts are considered safe. Previous studies have shown that the essential oils (EOs) of SM from different areas are rich in monoterpenes, sesquiterpenes, diterpens, and phenolic compounds, including flavonoids, tannins, and acids with great composition variability. Determination of composition of EO from Herzegovinian SM done by gas chromatography-flame ionization detection and gas chromatography mass spectrometry (GC-FID and GC/MS, respectively) revealed carvacrol as a primary substance followed by γ-terpinene, p-cymene, and β-caryophyllene. Ex vivo spasmolitic activity caused by EO was evident in different types of isolated rat ileum function with the most potent effect on spontaneous activity followed by electrical field stimulation and KCl-and CaCl2-induced activity. SMEO produced in vivo antidiarreal activity on castor oil-induced diarrhea in young rats and showed the potential to cause a decrese water content in the feces of adult Wistar rats.This study indicates that effects of SM on the intestinum could be mediated through combination of Kv channel activation and Ca channel blockade, but additional mechanisms might be involved. The results of this study corroborate the traditional use of SM as antispasmodic, antidiarrheal, and antisecretory agents.",
journal = "Records of Natural Products",
title = "Essential Oil of Satureja montana L. from Herzegovina: Assessment of Composition, Antispasmodic, and Antidiarrheal Effects",
number = "3",
volume = "17",
doi = "10.25135/rnp.358.2207.2522",
pages = "536-548"
}

Kulić, M., Drakul, D., Sokolović, D., Kordić-Bojinović, J., Milovanović, S.,& Blagojević, D.. (2023). Essential Oil of Satureja montana L. from Herzegovina: Assessment of Composition, Antispasmodic, and Antidiarrheal Effects. in Records of Natural Products, 17(3), 536-548.
https://doi.org/10.25135/rnp.358.2207.2522

Kulić M, Drakul D, Sokolović D, Kordić-Bojinović J, Milovanović S, Blagojević D. Essential Oil of Satureja montana L. from Herzegovina: Assessment of Composition, Antispasmodic, and Antidiarrheal Effects. in Records of Natural Products. 2023;17(3):536-548.
doi:10.25135/rnp.358.2207.2522 .

Kulić, Milan, Drakul, Dragana, Sokolović, Dragana, Kordić-Bojinović, Jelena, Milovanović, Slobodan, Blagojević, Duško, "Essential Oil of Satureja montana L. from Herzegovina: Assessment of Composition, Antispasmodic, and Antidiarrheal Effects" in Records of Natural Products, 17, no. 3 (2023):536-548,
https://doi.org/10.25135/rnp.358.2207.2522 . .

TY  - JOUR
AU  - Milosavljević, Filip
AU  - Brusini, Irene
AU  - Atanasov, Andrea
AU  - Manojlović, Marina
AU  - Vučić, Marija
AU  - Oreščanin-Dušić, Zorana
AU  - Brkljačić, Jelena
AU  - Miljević, Čedo
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Wang, Chunliang
AU  - Damberg, Peter
AU  - Pešić, Vesna
AU  - Tyndale, Rachel F.
AU  - Ingelman-Sundberg, Magnus
AU  - Jukić, Marin M.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5758
AB  - Aims: CYP2C19 transgenic mouse expresses the human CYP2C19 gene in the liver and developing brain, and it exhibits altered neurodevelopment associated with impairments in emotionality and locomotion. Because the validation of new animal models is essential for the understanding of the aetiology and pathophysiology of movement disorders, the objective was to characterise motoric phenotype in CYP2C19 transgenic mice and to investigate its validity as a new animal model of ataxia.

Methods: The rotarod, paw-print and beam-walking tests were utilised to characterise the motoric phenotype. The volumes of 20 brain regions in CYP2C19 transgenic and wild-type mice were quantified by 9.4T gadolinium-enhanced post-mortem structural neuroimaging. Antioxidative enzymatic activity was quantified biochemically. Dopaminergic alterations were characterised by chromatographic quantification of concentrations of dopamine and its metabolites and by subsequent immunohistochemical analyses. The beam-walking test was repeated after the treatment with dopamine receptor antagonists ecopipam and raclopride.

Results: CYP2C19 transgenic mice exhibit abnormal, unilateral ataxia-like gait, clasping reflex and 5.6-fold more paw-slips in the beam-walking test; the motoric phenotype was more pronounced in youth. Transgenic mice exhibited a profound reduction of 12% in cerebellar volume and a moderate reduction of 4% in hippocampal volume; both regions exhibited an increased antioxidative enzyme activity. CYP2C19 mice were hyperdopaminergic; however, the motoric impairment was not ameliorated by dopamine receptor antagonists, and there was no alteration in the number of midbrain dopaminergic neurons in CYP2C19 mice.

Conclusions: Humanised CYP2C19 transgenic mice exhibit altered gait and functional motoric impairments; this phenotype is likely caused by an aberrant cerebellar development.
PB  - Hoboken: Wiley
T2  - Neuropathology and Applied Neurobiology
T1  - The humanised CYP2C19 transgenic mouse exhibits cerebellar atrophy and movement impairment reminiscent of ataxia
IS  - 1
VL  - 49
DO  - 10.1111/nan.12867
SP  - e12867
ER  - 

@article{
author = "Milosavljević, Filip and Brusini, Irene and Atanasov, Andrea and Manojlović, Marina and Vučić, Marija and Oreščanin-Dušić, Zorana and Brkljačić, Jelena and Miljević, Čedo and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Wang, Chunliang and Damberg, Peter and Pešić, Vesna and Tyndale, Rachel F. and Ingelman-Sundberg, Magnus and Jukić, Marin M.",
year = "2023",
abstract = "Aims: CYP2C19 transgenic mouse expresses the human CYP2C19 gene in the liver and developing brain, and it exhibits altered neurodevelopment associated with impairments in emotionality and locomotion. Because the validation of new animal models is essential for the understanding of the aetiology and pathophysiology of movement disorders, the objective was to characterise motoric phenotype in CYP2C19 transgenic mice and to investigate its validity as a new animal model of ataxia.

Methods: The rotarod, paw-print and beam-walking tests were utilised to characterise the motoric phenotype. The volumes of 20 brain regions in CYP2C19 transgenic and wild-type mice were quantified by 9.4T gadolinium-enhanced post-mortem structural neuroimaging. Antioxidative enzymatic activity was quantified biochemically. Dopaminergic alterations were characterised by chromatographic quantification of concentrations of dopamine and its metabolites and by subsequent immunohistochemical analyses. The beam-walking test was repeated after the treatment with dopamine receptor antagonists ecopipam and raclopride.

Results: CYP2C19 transgenic mice exhibit abnormal, unilateral ataxia-like gait, clasping reflex and 5.6-fold more paw-slips in the beam-walking test; the motoric phenotype was more pronounced in youth. Transgenic mice exhibited a profound reduction of 12% in cerebellar volume and a moderate reduction of 4% in hippocampal volume; both regions exhibited an increased antioxidative enzyme activity. CYP2C19 mice were hyperdopaminergic; however, the motoric impairment was not ameliorated by dopamine receptor antagonists, and there was no alteration in the number of midbrain dopaminergic neurons in CYP2C19 mice.

Conclusions: Humanised CYP2C19 transgenic mice exhibit altered gait and functional motoric impairments; this phenotype is likely caused by an aberrant cerebellar development.",
publisher = "Hoboken: Wiley",
journal = "Neuropathology and Applied Neurobiology",
title = "The humanised CYP2C19 transgenic mouse exhibits cerebellar atrophy and movement impairment reminiscent of ataxia",
number = "1",
volume = "49",
doi = "10.1111/nan.12867",
pages = "e12867"
}

Milosavljević, F., Brusini, I., Atanasov, A., Manojlović, M., Vučić, M., Oreščanin-Dušić, Z., Brkljačić, J., Miljević, Č., Nikolić-Kokić, A., Blagojević, D., Wang, C., Damberg, P., Pešić, V., Tyndale, R. F., Ingelman-Sundberg, M.,& Jukić, M. M.. (2023). The humanised CYP2C19 transgenic mouse exhibits cerebellar atrophy and movement impairment reminiscent of ataxia. in Neuropathology and Applied Neurobiology
Hoboken: Wiley., 49(1), e12867.
https://doi.org/10.1111/nan.12867

Milosavljević F, Brusini I, Atanasov A, Manojlović M, Vučić M, Oreščanin-Dušić Z, Brkljačić J, Miljević Č, Nikolić-Kokić A, Blagojević D, Wang C, Damberg P, Pešić V, Tyndale RF, Ingelman-Sundberg M, Jukić MM. The humanised CYP2C19 transgenic mouse exhibits cerebellar atrophy and movement impairment reminiscent of ataxia. in Neuropathology and Applied Neurobiology. 2023;49(1):e12867.
doi:10.1111/nan.12867 .

Milosavljević, Filip, Brusini, Irene, Atanasov, Andrea, Manojlović, Marina, Vučić, Marija, Oreščanin-Dušić, Zorana, Brkljačić, Jelena, Miljević, Čedo, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Wang, Chunliang, Damberg, Peter, Pešić, Vesna, Tyndale, Rachel F., Ingelman-Sundberg, Magnus, Jukić, Marin M., "The humanised CYP2C19 transgenic mouse exhibits cerebellar atrophy and movement impairment reminiscent of ataxia" in Neuropathology and Applied Neurobiology, 49, no. 1 (2023):e12867,
https://doi.org/10.1111/nan.12867 . .

TY  - CONF
AU  - Grahovac, Tanja
AU  - Tatalović, Nikola
AU  - Vidonja Uzelac, Teodora
AU  - Slavić, Marija
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Oreščanin-Dušić, Zorana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5848
AB  - Женке пацова имају хормонски контролисан еструсни циклус који изазива регуларне цикличне ткивне и ћелијске промене. Стога, експерименти који се изводе на женкама треба да укључују проверу фазе циклуса и рад на јединкама које су у истој фази. Уобичајено је да се узимају женке у еструсу, али како еструсна фаза траје 15-24 h, женке третиране у еструсу после 6 h ће бити и даље у еструсу у већини случајева, али већ после 24 h су у метаеструсу. Наши претходни резултати показују да ефекти на женкама пацова зависе од фазе циклуса када је апликација фармаколошки активним агенсом урађена. Како је изоловани утерус један од фармаколошких ex vivo модела избора испитивања деловања фармаколошки активних супстанци, укључујући и редокс активне, промене у утерусу директно подложне цикличности могу утицати на ефективност учинка, те и давати варијабилне резултате активности. Стога смо у овом раду мерили активност антиоксидативних ензима (SOD, CAT, GSH-Px и GR) у утерусу у различитим фазама еструсног циклуса. Утеруси у еструсу имају нижу SOD2 и вишу GSH-Px активност у поређењу са другим фазама. Активност GR у еструсу и проеструсу су биле више у поређењу са метаеструсом и диеструсом. Ове промене су у вези и са хормоналним и са редокс статусом утеруса током циклуса. Наши резултати показују да физиолошки, а посебно редокс одговор на екстерне стимулусе може бити другачији у зависности од фазе еструсног циклуса.
AB  - Ženke pacova imaju hormonski kontrolisan estrusni ciklus koji izaziva regularne ciklične tkivne i ćelijske promene. Stoga, eksperimenti koji se izvode na ženkama treba da uključuje proveru faze ciklusa i rad na jedinkama koje su u istoj fazi. Uobičajeno je da se uzimaju ženke u estrusu, ali kako estrusna faza traje 15-24 h, ženke tretirane u estrusu posle 6 h će biti i dalje u estrusu u većini slučajeva, ali već posle 24 h su u metaestrusu. Naši prethodni rezultati pokazuju da efekti na ženkama pacova zavise od faze ciklusa kada je aplikacija farmakološki aktivnim agensom urađena. Kako je izolovani uterus jedan od farmakoloških ex vivo modela izbora ispitivanja delovanja farmakološki aktivnih supstanci, uključujući i redoks aktivne, promene u uterusu direktno podložne cikličnosti mogu uticati na efektivnost učinka, te i davati varijabilne rezultate aktivnosti. Stoga smo u ovom radu merili aktivnost antioksidativnih enzima (SOD, CAT, GSH-Px и GR) u uterusu u različitim fazama estrusnog ciklusa. Uterusi u estrusu imaju nižu SOD2 i višu GSH-Px aktivnost u poređenju sa drugim fazama. Aktivnost GR u estrusu i proestrusu su bile više u poređenju sa metaestrusom i diestrusom. Ove promene su u vezi i sa hormonalnim i sa redoks statusom uterusa tokom ciklusa. Naši rezultati pokazuju da fiziološki, a posebno redoks odgovor na eksterne stimuluse može biti drugačiji u zavisnosti od faze estrusnog ciklusa.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Активност антиоксидативних ензима у утерусу је зависна од еструсног циклуса
T1  - Aktivnost antioksidativnih enzima u uterusu je zavisna od estrusnog ciklusa
SP  - 390
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5848
ER  - 

@conference{
author = "Grahovac, Tanja and Tatalović, Nikola and Vidonja Uzelac, Teodora and Slavić, Marija and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Oreščanin-Dušić, Zorana",
year = "2022",
abstract = "Женке пацова имају хормонски контролисан еструсни циклус који изазива регуларне цикличне ткивне и ћелијске промене. Стога, експерименти који се изводе на женкама треба да укључују проверу фазе циклуса и рад на јединкама које су у истој фази. Уобичајено је да се узимају женке у еструсу, али како еструсна фаза траје 15-24 h, женке третиране у еструсу после 6 h ће бити и даље у еструсу у већини случајева, али већ после 24 h су у метаеструсу. Наши претходни резултати показују да ефекти на женкама пацова зависе од фазе циклуса када је апликација фармаколошки активним агенсом урађена. Како је изоловани утерус један од фармаколошких ex vivo модела избора испитивања деловања фармаколошки активних супстанци, укључујући и редокс активне, промене у утерусу директно подложне цикличности могу утицати на ефективност учинка, те и давати варијабилне резултате активности. Стога смо у овом раду мерили активност антиоксидативних ензима (SOD, CAT, GSH-Px и GR) у утерусу у различитим фазама еструсног циклуса. Утеруси у еструсу имају нижу SOD2 и вишу GSH-Px активност у поређењу са другим фазама. Активност GR у еструсу и проеструсу су биле више у поређењу са метаеструсом и диеструсом. Ове промене су у вези и са хормоналним и са редокс статусом утеруса током циклуса. Наши резултати показују да физиолошки, а посебно редокс одговор на екстерне стимулусе може бити другачији у зависности од фазе еструсног циклуса., Ženke pacova imaju hormonski kontrolisan estrusni ciklus koji izaziva regularne ciklične tkivne i ćelijske promene. Stoga, eksperimenti koji se izvode na ženkama treba da uključuje proveru faze ciklusa i rad na jedinkama koje su u istoj fazi. Uobičajeno je da se uzimaju ženke u estrusu, ali kako estrusna faza traje 15-24 h, ženke tretirane u estrusu posle 6 h će biti i dalje u estrusu u većini slučajeva, ali već posle 24 h su u metaestrusu. Naši prethodni rezultati pokazuju da efekti na ženkama pacova zavise od faze ciklusa kada je aplikacija farmakološki aktivnim agensom urađena. Kako je izolovani uterus jedan od farmakoloških ex vivo modela izbora ispitivanja delovanja farmakološki aktivnih supstanci, uključujući i redoks aktivne, promene u uterusu direktno podložne cikličnosti mogu uticati na efektivnost učinka, te i davati varijabilne rezultate aktivnosti. Stoga smo u ovom radu merili aktivnost antioksidativnih enzima (SOD, CAT, GSH-Px и GR) u uterusu u različitim fazama estrusnog ciklusa. Uterusi u estrusu imaju nižu SOD2 i višu GSH-Px aktivnost u poređenju sa drugim fazama. Aktivnost GR u estrusu i proestrusu su bile više u poređenju sa metaestrusom i diestrusom. Ove promene su u vezi i sa hormonalnim i sa redoks statusom uterusa tokom ciklusa. Naši rezultati pokazuju da fiziološki, a posebno redoks odgovor na eksterne stimuluse može biti drugačiji u zavisnosti od faze estrusnog ciklusa.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Активност антиоксидативних ензима у утерусу је зависна од еструсног циклуса, Aktivnost antioksidativnih enzima u uterusu je zavisna od estrusnog ciklusa",
pages = "390",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5848"
}

Grahovac, T., Tatalović, N., Vidonja Uzelac, T., Slavić, M., Nikolić-Kokić, A., Blagojević, D.,& Oreščanin-Dušić, Z.. (2022). Активност антиоксидативних ензима у утерусу је зависна од еструсног циклуса. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 390.
https://hdl.handle.net/21.15107/rcub_ibiss_5848

Grahovac T, Tatalović N, Vidonja Uzelac T, Slavić M, Nikolić-Kokić A, Blagojević D, Oreščanin-Dušić Z. Активност антиоксидативних ензима у утерусу је зависна од еструсног циклуса. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:390.
https://hdl.handle.net/21.15107/rcub_ibiss_5848 .

Grahovac, Tanja, Tatalović, Nikola, Vidonja Uzelac, Teodora, Slavić, Marija, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Oreščanin-Dušić, Zorana, "Активност антиоксидативних ензима у утерусу је зависна од еструсног циклуса" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):390,
https://hdl.handle.net/21.15107/rcub_ibiss_5848 .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Tatalović, Nikola
AU  - Brkljačić, Jelena
AU  - Mijović, Milica
AU  - Nestorović, Vojkan
AU  - Mijušković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić, Milan
AU  - Spasić, Snežana
AU  - Blagojević, Duško
AU  - Miljević, Čedo
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/22/13698
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5344
AB  - Sexual dysfunction, as a noticeable adverse effect of atypical antipsychotic drugs (APDs) for the treatment of schizophrenia, has not been investigated in detail. A study was undertaken to investigate whether 28-day long treatment with clozapine, ziprasidone or sertindole (using a recommended daily dose for atypical antipsychotic therapy), induced histopathological changes both in rat testicles and prostate, changed the activity of the antioxidant defence system and altered blood testosterone and prolactin. Clozapine, ziprasidone and sertindole induced histopathological changes in rat testicular tissue, which could be attributed to a disturbed testicular antioxidant defence system in addition to an altered prolactin to testosterone ratio. None of the APD treatments induced histopathological changes in prostate. Our results demonstrate that APDs have the capacity to change both redox and endocrinological balance. One or both outcomes could underline testicular degeneration and disturbed spermatogenesis.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis
IS  - 22
VL  - 23
DO  - 10.3390/ijms232213698
SP  - 13698
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Tatalović, Nikola and Brkljačić, Jelena and Mijović, Milica and Nestorović, Vojkan and Mijušković, Ana and Oreščanin-Dušić, Zorana and Vidonja Uzelac, Teodora and Nikolić, Milan and Spasić, Snežana and Blagojević, Duško and Miljević, Čedo",
year = "2022",
abstract = "Sexual dysfunction, as a noticeable adverse effect of atypical antipsychotic drugs (APDs) for the treatment of schizophrenia, has not been investigated in detail. A study was undertaken to investigate whether 28-day long treatment with clozapine, ziprasidone or sertindole (using a recommended daily dose for atypical antipsychotic therapy), induced histopathological changes both in rat testicles and prostate, changed the activity of the antioxidant defence system and altered blood testosterone and prolactin. Clozapine, ziprasidone and sertindole induced histopathological changes in rat testicular tissue, which could be attributed to a disturbed testicular antioxidant defence system in addition to an altered prolactin to testosterone ratio. None of the APD treatments induced histopathological changes in prostate. Our results demonstrate that APDs have the capacity to change both redox and endocrinological balance. One or both outcomes could underline testicular degeneration and disturbed spermatogenesis.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis",
number = "22",
volume = "23",
doi = "10.3390/ijms232213698",
pages = "13698"
}

Nikolić-Kokić, A., Tatalović, N., Brkljačić, J., Mijović, M., Nestorović, V., Mijušković, A., Oreščanin-Dušić, Z., Vidonja Uzelac, T., Nikolić, M., Spasić, S., Blagojević, D.,& Miljević, Č.. (2022). Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis. in International Journal of Molecular Sciences
Basel: MDPI., 23(22), 13698.
https://doi.org/10.3390/ijms232213698

Nikolić-Kokić A, Tatalović N, Brkljačić J, Mijović M, Nestorović V, Mijušković A, Oreščanin-Dušić Z, Vidonja Uzelac T, Nikolić M, Spasić S, Blagojević D, Miljević Č. Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis. in International Journal of Molecular Sciences. 2022;23(22):13698.
doi:10.3390/ijms232213698 .

Nikolić-Kokić, Aleksandra, Tatalović, Nikola, Brkljačić, Jelena, Mijović, Milica, Nestorović, Vojkan, Mijušković, Ana, Oreščanin-Dušić, Zorana, Vidonja Uzelac, Teodora, Nikolić, Milan, Spasić, Snežana, Blagojević, Duško, Miljević, Čedo, "Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis" in International Journal of Molecular Sciences, 23, no. 22 (2022):13698,
https://doi.org/10.3390/ijms232213698 . .

TY  - JOUR
AU  - Tasić, Danica
AU  - Opačić, Miloš
AU  - Kovačević, Sanja
AU  - Nikolić-Kokić, Aleksandra
AU  - Dimitrijević, Milena
AU  - Nikolić, Dušan
AU  - Vojnović-Milutinović, Danijela
AU  - Blagojević, Duško
AU  - Đorđević, Ana
AU  - Brkljačić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5025
AB  - The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys
are still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression
of metabolic disturbances in the kidney. We determined protein level of copper transporters,
chaperones, and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes
function in the kidneys of male Wistar rats subjected to 20% liquid fructose supplementation
and/or chronic stress. Liquid fructose supplementation increased level of copper chaperone of
superoxide dismutase and decreased metallothionein level, while rendering the level of copper
importer and copper chaperones involved in copper delivery to mitochondria and trans Golgi
network unaffected. Stress had no effect on renal copper metabolism. The activity and expression
of renal antioxidant enzymes remained unaltered in all experimental groups. In conclusion, fructose,
independently of stress, decreased renal copper level, and modulated renal copper metabolism
as to preserve vital cellular function including mitochondrial energy production and antioxidative
defense, at the expense of intracellular copper storage.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function
IS  - 16
VL  - 23
DO  - 10.3390/ijms23169023
SP  - 9023
ER  - 

@article{
author = "Tasić, Danica and Opačić, Miloš and Kovačević, Sanja and Nikolić-Kokić, Aleksandra and Dimitrijević, Milena and Nikolić, Dušan and Vojnović-Milutinović, Danijela and Blagojević, Duško and Đorđević, Ana and Brkljačić, Jelena",
year = "2022",
abstract = "The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys
are still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression
of metabolic disturbances in the kidney. We determined protein level of copper transporters,
chaperones, and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes
function in the kidneys of male Wistar rats subjected to 20% liquid fructose supplementation
and/or chronic stress. Liquid fructose supplementation increased level of copper chaperone of
superoxide dismutase and decreased metallothionein level, while rendering the level of copper
importer and copper chaperones involved in copper delivery to mitochondria and trans Golgi
network unaffected. Stress had no effect on renal copper metabolism. The activity and expression
of renal antioxidant enzymes remained unaltered in all experimental groups. In conclusion, fructose,
independently of stress, decreased renal copper level, and modulated renal copper metabolism
as to preserve vital cellular function including mitochondrial energy production and antioxidative
defense, at the expense of intracellular copper storage.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function",
number = "16",
volume = "23",
doi = "10.3390/ijms23169023",
pages = "9023"
}

Tasić, D., Opačić, M., Kovačević, S., Nikolić-Kokić, A., Dimitrijević, M., Nikolić, D., Vojnović-Milutinović, D., Blagojević, D., Đorđević, A.,& Brkljačić, J.. (2022). Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences
Basel: MDPI., 23(16), 9023.
https://doi.org/10.3390/ijms23169023

Tasić D, Opačić M, Kovačević S, Nikolić-Kokić A, Dimitrijević M, Nikolić D, Vojnović-Milutinović D, Blagojević D, Đorđević A, Brkljačić J. Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences. 2022;23(16):9023.
doi:10.3390/ijms23169023 .

Tasić, Danica, Opačić, Miloš, Kovačević, Sanja, Nikolić-Kokić, Aleksandra, Dimitrijević, Milena, Nikolić, Dušan, Vojnović-Milutinović, Danijela, Blagojević, Duško, Đorđević, Ana, Brkljačić, Jelena, "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function" in International Journal of Molecular Sciences, 23, no. 16 (2022):9023,
https://doi.org/10.3390/ijms23169023 . .

TY  - CONF
AU  - Guševac Stojanović, Ivana
AU  - Tatalović, Nikola
AU  - Veljković, Filip
AU  - Martinović, Jelena
AU  - Zarić Kontić, Marina
AU  - Blagojević, Duško
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
PY  - 2022
UR  - https://www.ecnp.eu/congress2022/ECNPcongress/programme/programme#!abstractdetails/0000506150
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5315
AB  - Introduction: Cerebral hypoperfusion (CH), a reduced cerebral blood flow, characteristic for aging and neurodegenerative disorders, might be associated with a pro-oxidative state in the brain due to the generation of reactive oxygen species (ROS) that cause neuronal damage. These overgenerated pro-oxidants might be reduced, disabled and neutralized by the endogenous antioxidant enzymes, including superoxide dismutase (SOD) and catalase (CAT). A sex steroid hormone, progesterone, in several experimental models of neuronal injury exerts anti-oxidative properties upregulating anti-oxidative molecules and thus promoting neuroprotection. However, its efficacy in the state of CH is still unknown and it is necessary to better understand the mechanism underlying this hormone’s neuroprotective effects.
Aim: We investigated whether progesterone is able to modulate CH-induced pro-oxidative/anti-oxidative imbalance, as well as SOD and CAT activities, in the prefrontal cortex of three-month-old male Wistar rats subjected to permanent bilateral common carotid artery occlusion (2VO).
Methods: The appropriate actions were undertaken to reduce the distress of the animals in agreement with the European Community Council Directive of 86/609/EEC and 010/63/EU for animal experiments. All experimental procedures were allowed by the Ethical Committee for the Use of Laboratory Animals of VINČA Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Republic of Serbia (protocol authorization numbers 02/11 and 323-07-04253/2016-05). Following chloral hydrate anaesthesia, 8 rats randomly assigned in 2VO groups were double occluded with 5–0 silk suture, while 4 animals from sham operated group were subjected to the same surgical procedure but without actual ligation of carotid arteries. Four hours following the surgical procedure animals from sham operated group, used as controls, were subcutaneously treated with vehicle (commercial flax oil, 1 mg/kg/day, S + V), while animals in 2VO groups were subjected to either progesterone (1.7 mg/kg/day, 2VO + P) or vehicle (commercial flax oil, 1 mg/kg/day, 2VO + V). Following 7 days lasting treatments, pro-oxidative/anti-oxidative balance was assessed according to the method of Alamdari et al. [1]; while activities of anti-oxidative enzymes were determined as described in the study by Tatalović et al. [2]. One-way analysis of variance (ANOVA) test followed by Tukey’s post hoc test was employed to analyse data. The values of p < 0.05 or less were considered statistically significant.
Results: Obtained results demonstrate that compared to controls, 2VO and vehicle treatment induced a significant increase of pro-oxidants levels (p < 0.01) as well as the activities of both investigated anti-oxidative enzymes, although only the CAT activity was significantly increased (p < 0.05). Following progesterone treatment, pro-oxidants levels were lowered to the control level which caused the difference between the 2VO groups (p < 0.05). In parallel, no statistically significant change in SOD activity was observed, while CAT was upregulated (p < 0.05).
Conclusion: Presented results suggest that in the prefrontal cortex in the state of CH, progesterone, by modulating the CAT activity, downregulates overgenerated reactive species, reduces CH-induced oxidative stress and exerts anti-oxidative outcomes.
The Ministry of Education, Science and Technological Development


Disclosure statement: Financially supported by The Ministry of Education, Science and Technological Development, grants No 451-03-1/2022-14/13 and 451-03-68/2022-14/ 200007 and 13451-03-68/2022-14/ 200175




 
[1] Alamdari, D.H., Ghayour-Mobarhan, M., Tavallaie, S., Parizadeh, M.R., Moohebati, M., Ghafoori, F., et al., 2008. Prooxidant–antioxidant balance as a new risk factor in patients with angiographically defined coronary artery disease. Clinical Biochemistry 41(6),375–380. 
[2] Tatalović, N., Vidonja Uzelac, T., Oreščanin Dušić, Z., Nikolić-Kokić, A., Bresjanac, M., Blagojević, D., 2021. Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated. Antioxidants 10(11),1792.
PB  - European College of Neuropsychopharmacology (ECNP)
C3  - The 35th ECNP Congress; 2023 Oct 7-10; Barcelona, Spain
T1  - Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model
IS  - P.0477
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5315
ER  - 

@conference{
author = "Guševac Stojanović, Ivana and Tatalović, Nikola and Veljković, Filip and Martinović, Jelena and Zarić Kontić, Marina and Blagojević, Duško and Stojanović, Zoran and Drakulić, Dunja",
year = "2022",
abstract = "Introduction: Cerebral hypoperfusion (CH), a reduced cerebral blood flow, characteristic for aging and neurodegenerative disorders, might be associated with a pro-oxidative state in the brain due to the generation of reactive oxygen species (ROS) that cause neuronal damage. These overgenerated pro-oxidants might be reduced, disabled and neutralized by the endogenous antioxidant enzymes, including superoxide dismutase (SOD) and catalase (CAT). A sex steroid hormone, progesterone, in several experimental models of neuronal injury exerts anti-oxidative properties upregulating anti-oxidative molecules and thus promoting neuroprotection. However, its efficacy in the state of CH is still unknown and it is necessary to better understand the mechanism underlying this hormone’s neuroprotective effects.
Aim: We investigated whether progesterone is able to modulate CH-induced pro-oxidative/anti-oxidative imbalance, as well as SOD and CAT activities, in the prefrontal cortex of three-month-old male Wistar rats subjected to permanent bilateral common carotid artery occlusion (2VO).
Methods: The appropriate actions were undertaken to reduce the distress of the animals in agreement with the European Community Council Directive of 86/609/EEC and 010/63/EU for animal experiments. All experimental procedures were allowed by the Ethical Committee for the Use of Laboratory Animals of VINČA Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Republic of Serbia (protocol authorization numbers 02/11 and 323-07-04253/2016-05). Following chloral hydrate anaesthesia, 8 rats randomly assigned in 2VO groups were double occluded with 5–0 silk suture, while 4 animals from sham operated group were subjected to the same surgical procedure but without actual ligation of carotid arteries. Four hours following the surgical procedure animals from sham operated group, used as controls, were subcutaneously treated with vehicle (commercial flax oil, 1 mg/kg/day, S + V), while animals in 2VO groups were subjected to either progesterone (1.7 mg/kg/day, 2VO + P) or vehicle (commercial flax oil, 1 mg/kg/day, 2VO + V). Following 7 days lasting treatments, pro-oxidative/anti-oxidative balance was assessed according to the method of Alamdari et al. [1]; while activities of anti-oxidative enzymes were determined as described in the study by Tatalović et al. [2]. One-way analysis of variance (ANOVA) test followed by Tukey’s post hoc test was employed to analyse data. The values of p < 0.05 or less were considered statistically significant.
Results: Obtained results demonstrate that compared to controls, 2VO and vehicle treatment induced a significant increase of pro-oxidants levels (p < 0.01) as well as the activities of both investigated anti-oxidative enzymes, although only the CAT activity was significantly increased (p < 0.05). Following progesterone treatment, pro-oxidants levels were lowered to the control level which caused the difference between the 2VO groups (p < 0.05). In parallel, no statistically significant change in SOD activity was observed, while CAT was upregulated (p < 0.05).
Conclusion: Presented results suggest that in the prefrontal cortex in the state of CH, progesterone, by modulating the CAT activity, downregulates overgenerated reactive species, reduces CH-induced oxidative stress and exerts anti-oxidative outcomes.
The Ministry of Education, Science and Technological Development


Disclosure statement: Financially supported by The Ministry of Education, Science and Technological Development, grants No 451-03-1/2022-14/13 and 451-03-68/2022-14/ 200007 and 13451-03-68/2022-14/ 200175




 
[1] Alamdari, D.H., Ghayour-Mobarhan, M., Tavallaie, S., Parizadeh, M.R., Moohebati, M., Ghafoori, F., et al., 2008. Prooxidant–antioxidant balance as a new risk factor in patients with angiographically defined coronary artery disease. Clinical Biochemistry 41(6),375–380. 
[2] Tatalović, N., Vidonja Uzelac, T., Oreščanin Dušić, Z., Nikolić-Kokić, A., Bresjanac, M., Blagojević, D., 2021. Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated. Antioxidants 10(11),1792.",
publisher = "European College of Neuropsychopharmacology (ECNP)",
journal = "The 35th ECNP Congress; 2023 Oct 7-10; Barcelona, Spain",
title = "Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model",
number = "P.0477",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5315"
}

Guševac Stojanović, I., Tatalović, N., Veljković, F., Martinović, J., Zarić Kontić, M., Blagojević, D., Stojanović, Z.,& Drakulić, D.. (2022). Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model. in The 35th ECNP Congress; 2023 Oct 7-10; Barcelona, Spain
European College of Neuropsychopharmacology (ECNP).(P.0477).
https://hdl.handle.net/21.15107/rcub_ibiss_5315

Guševac Stojanović I, Tatalović N, Veljković F, Martinović J, Zarić Kontić M, Blagojević D, Stojanović Z, Drakulić D. Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model. in The 35th ECNP Congress; 2023 Oct 7-10; Barcelona, Spain. 2022;(P.0477).
https://hdl.handle.net/21.15107/rcub_ibiss_5315 .

Guševac Stojanović, Ivana, Tatalović, Nikola, Veljković, Filip, Martinović, Jelena, Zarić Kontić, Marina, Blagojević, Duško, Stojanović, Zoran, Drakulić, Dunja, "Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model" in The 35th ECNP Congress; 2023 Oct 7-10; Barcelona, Spain, no. P.0477 (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5315 .

TY  - CONF
AU  - Tatalović, Nikola
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Grahovac, Tanja
AU  - Blagojević, Duško
AU  - Miljević, Čedo
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5186
AB  - Atypical antipsychotic drugs (APDs) are being used to treat
acute psychotic episodes in schizophrenia and schizophrenia-related
diseases as well as a variety of nonpsychotic disorders.
Despite their effectiveness, we showed that APDs clozapine
(CLO), ziprasidone (ZIP) and sertindole (SER) increased oxidative
stress and reduced antioxidative defence capacity in rat kidneys
and heart. Given the scarcity of data about CLO, ZIP and
SER actions on the brain redox homeostasis, the goal of current
study was to investigate their impact on antioxidant enzymes:
total superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GPx) and glutathione reductase (GR) in brain of 3 month old male Wistar rats treated daily via intubation with
water (control group), CLO (45 mg/kg/day), ZIP (20 mg/kg/day)
or SER (2.5 mg/kg/day) for 6 weeks. There were no significant
changes in investigated parameters in CLO and ZIP groups.
However, in SER group the total SOD activity was decreased
while CAT and GPx activities were increased compared to control
group (One-way ANOVA with Tukey’s HSD test, P < 0.05).
Since both CAT and GPx catalyze decomposition of H2O2, their
increased activity in SER group suggests an elevated peroxide
pressure. This conclusion can be strengthened since the activity
of SOD can be decreased by elevated concentration of H2O2.
Our results suggest that SER could cause a redox imbalance in
brain with possible negative effects on mitochondrial respiration
and neural tissue metabolism. *The authors marked with an
asterisk equally contributed to the work.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Chronic treatment with sertindole, but not clozapine and ziprasidone, affects redox homeostasis in brain of male rats
DO  - 10.1002/2211-5463.13440
SP  - 299
ER  - 

@conference{
author = "Tatalović, Nikola and Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Vidonja Uzelac, Teodora and Grahovac, Tanja and Blagojević, Duško and Miljević, Čedo",
year = "2022",
abstract = "Atypical antipsychotic drugs (APDs) are being used to treat
acute psychotic episodes in schizophrenia and schizophrenia-related
diseases as well as a variety of nonpsychotic disorders.
Despite their effectiveness, we showed that APDs clozapine
(CLO), ziprasidone (ZIP) and sertindole (SER) increased oxidative
stress and reduced antioxidative defence capacity in rat kidneys
and heart. Given the scarcity of data about CLO, ZIP and
SER actions on the brain redox homeostasis, the goal of current
study was to investigate their impact on antioxidant enzymes:
total superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GPx) and glutathione reductase (GR) in brain of 3 month old male Wistar rats treated daily via intubation with
water (control group), CLO (45 mg/kg/day), ZIP (20 mg/kg/day)
or SER (2.5 mg/kg/day) for 6 weeks. There were no significant
changes in investigated parameters in CLO and ZIP groups.
However, in SER group the total SOD activity was decreased
while CAT and GPx activities were increased compared to control
group (One-way ANOVA with Tukey’s HSD test, P < 0.05).
Since both CAT and GPx catalyze decomposition of H2O2, their
increased activity in SER group suggests an elevated peroxide
pressure. This conclusion can be strengthened since the activity
of SOD can be decreased by elevated concentration of H2O2.
Our results suggest that SER could cause a redox imbalance in
brain with possible negative effects on mitochondrial respiration
and neural tissue metabolism. *The authors marked with an
asterisk equally contributed to the work.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Chronic treatment with sertindole, but not clozapine and ziprasidone, affects redox homeostasis in brain of male rats",
doi = "10.1002/2211-5463.13440",
pages = "299"
}

Tatalović, N., Nikolić-Kokić, A., Oreščanin Dušić, Z., Vidonja Uzelac, T., Grahovac, T., Blagojević, D.,& Miljević, Č.. (2022). Chronic treatment with sertindole, but not clozapine and ziprasidone, affects redox homeostasis in brain of male rats. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 299.
https://doi.org/10.1002/2211-5463.13440

Tatalović N, Nikolić-Kokić A, Oreščanin Dušić Z, Vidonja Uzelac T, Grahovac T, Blagojević D, Miljević Č. Chronic treatment with sertindole, but not clozapine and ziprasidone, affects redox homeostasis in brain of male rats. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:299.
doi:10.1002/2211-5463.13440 .

Tatalović, Nikola, Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Vidonja Uzelac, Teodora, Grahovac, Tanja, Blagojević, Duško, Miljević, Čedo, "Chronic treatment with sertindole, but not clozapine and ziprasidone, affects redox homeostasis in brain of male rats" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):299,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - CONF
AU  - Grahovac, Tanja
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Blagojević, Duško
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5185
AB  - Redox-mediated mechanisms play an important role in regulating
smooth muscle contractility and depend on subtle redox homeostasis
maintaining. Redox homeostasis in cells is obtained by
the activity of antioxidant enzymes. Among them, glutathione
reductase constantly reduces oxidized glutathione, providing a
favorable cellular redox milieu. The aim of this study was to
examine whether, to what extent and in what way changes in
smooth muscle contractility occurred in conditions when glutathione
reductase activity was changed. The experiments were
performed on isolated ileum of male Wistar rats, electrically stimulated
and treated with cumulative doses of carmustine, a glutathione
reductase inhibitor. The activity of antioxidant enzymes(catalase, glutathione reductase and glutathione peroxidase, as
well as the amount of total SH groups and glutathione) was
determined in treated ileum. The addition of carmustine inhibited
glutathione reductase activity and decreased amplitude and frequency
of electrically stimulated ileum. The results showed that
glutathione reductase activity and maintenance of highly reductive
cellular environment were essential to contractility processes.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Cellular redox homeostasis mediates contractility of isolated ileum of male Wistar rats
DO  - 10.1002/2211-5463.13440
SP  - 220
EP  - 221
ER  - 

@conference{
author = "Grahovac, Tanja and Vidonja Uzelac, Teodora and Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Blagojević, Duško",
year = "2022",
abstract = "Redox-mediated mechanisms play an important role in regulating
smooth muscle contractility and depend on subtle redox homeostasis
maintaining. Redox homeostasis in cells is obtained by
the activity of antioxidant enzymes. Among them, glutathione
reductase constantly reduces oxidized glutathione, providing a
favorable cellular redox milieu. The aim of this study was to
examine whether, to what extent and in what way changes in
smooth muscle contractility occurred in conditions when glutathione
reductase activity was changed. The experiments were
performed on isolated ileum of male Wistar rats, electrically stimulated
and treated with cumulative doses of carmustine, a glutathione
reductase inhibitor. The activity of antioxidant enzymes(catalase, glutathione reductase and glutathione peroxidase, as
well as the amount of total SH groups and glutathione) was
determined in treated ileum. The addition of carmustine inhibited
glutathione reductase activity and decreased amplitude and frequency
of electrically stimulated ileum. The results showed that
glutathione reductase activity and maintenance of highly reductive
cellular environment were essential to contractility processes.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Cellular redox homeostasis mediates contractility of isolated ileum of male Wistar rats",
doi = "10.1002/2211-5463.13440",
pages = "220-221"
}

Grahovac, T., Vidonja Uzelac, T., Nikolić-Kokić, A., Oreščanin Dušić, Z.,& Blagojević, D.. (2022). Cellular redox homeostasis mediates contractility of isolated ileum of male Wistar rats. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 220-221.
https://doi.org/10.1002/2211-5463.13440

Grahovac T, Vidonja Uzelac T, Nikolić-Kokić A, Oreščanin Dušić Z, Blagojević D. Cellular redox homeostasis mediates contractility of isolated ileum of male Wistar rats. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:220-221.
doi:10.1002/2211-5463.13440 .

Grahovac, Tanja, Vidonja Uzelac, Teodora, Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Blagojević, Duško, "Cellular redox homeostasis mediates contractility of isolated ileum of male Wistar rats" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):220-221,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - JOUR
AU  - Platanić Arizanović, Lena
AU  - Nikolić-Kokić, Aleksandra
AU  - Brkljačić, Jelena
AU  - Tatalović, Nikola
AU  - Miler, Marko
AU  - Oreščanin Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić, Milan
AU  - Milošević, Verica
AU  - Blagojević, Duško
AU  - Spasić, Snežana
AU  - Miljević, Čedo
PY  - 2021
UR  - https://www.tandfonline.com/doi/abs/10.1080/15287394.2020.1844827
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4037
AB  - Chronic use of atypical antipsychotics may produce hepatic damage. Atypical antipsychotics, including clozapine, sertindole, and ziprasidone, are extensively metabolized by the liver and this process generates toxic-free radical metabolic intermediates which may contribute to liver damage. The aim of this study was to investigate whether clozapine, sertindole, or ziprasidone affected hepatic antioxidant defense enzymes which consequently led to disturbed redox homeostasis. The expression and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferases (GST) were measured in rat livers at doses corresponding to human antipsychotic therapy. Clozapine increased activity of SOD types 1 and 2, GR and GST, but reduced CAT activity. Sertindole elevated activities of both SODs. In ziprasidone-treated rats only decreased CAT activity was found. All three antipsychotics produced mild-to-moderate hepatic histopathological changes categorized as regenerative alterations. No apparent signs of immune cell infiltration, microvesicular or macrovesicular fatty change, or hepatocytes in mitosis were observed. In conclusion, a 4-week long daily treatment with clozapine, sertindole, or ziprasidone altered hepatic antioxidant enzyme activities and induced histopathological changes in liver. The most severe alterations were noted in clozapine-treated rats. Data indicate that redox disturbances may contribute to liver dysfunction after long-term atypical antipsychotic drug treatment.
PB  - Bellwether Publishing, Ltd.
T2  - Journal of Toxicology and Environmental Health, Part A
T1  - Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction
IS  - 4
VL  - 84
DO  - 10.1080/15287394.2020.1844827
SP  - 173
EP  - 182
ER  - 

@article{
author = "Platanić Arizanović, Lena and Nikolić-Kokić, Aleksandra and Brkljačić, Jelena and Tatalović, Nikola and Miler, Marko and Oreščanin Dušić, Zorana and Vidonja Uzelac, Teodora and Nikolić, Milan and Milošević, Verica and Blagojević, Duško and Spasić, Snežana and Miljević, Čedo",
year = "2021",
abstract = "Chronic use of atypical antipsychotics may produce hepatic damage. Atypical antipsychotics, including clozapine, sertindole, and ziprasidone, are extensively metabolized by the liver and this process generates toxic-free radical metabolic intermediates which may contribute to liver damage. The aim of this study was to investigate whether clozapine, sertindole, or ziprasidone affected hepatic antioxidant defense enzymes which consequently led to disturbed redox homeostasis. The expression and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferases (GST) were measured in rat livers at doses corresponding to human antipsychotic therapy. Clozapine increased activity of SOD types 1 and 2, GR and GST, but reduced CAT activity. Sertindole elevated activities of both SODs. In ziprasidone-treated rats only decreased CAT activity was found. All three antipsychotics produced mild-to-moderate hepatic histopathological changes categorized as regenerative alterations. No apparent signs of immune cell infiltration, microvesicular or macrovesicular fatty change, or hepatocytes in mitosis were observed. In conclusion, a 4-week long daily treatment with clozapine, sertindole, or ziprasidone altered hepatic antioxidant enzyme activities and induced histopathological changes in liver. The most severe alterations were noted in clozapine-treated rats. Data indicate that redox disturbances may contribute to liver dysfunction after long-term atypical antipsychotic drug treatment.",
publisher = "Bellwether Publishing, Ltd.",
journal = "Journal of Toxicology and Environmental Health, Part A",
title = "Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction",
number = "4",
volume = "84",
doi = "10.1080/15287394.2020.1844827",
pages = "173-182"
}

Platanić Arizanović, L., Nikolić-Kokić, A., Brkljačić, J., Tatalović, N., Miler, M., Oreščanin Dušić, Z., Vidonja Uzelac, T., Nikolić, M., Milošević, V., Blagojević, D., Spasić, S.,& Miljević, Č.. (2021). Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction. in Journal of Toxicology and Environmental Health, Part A
Bellwether Publishing, Ltd.., 84(4), 173-182.
https://doi.org/10.1080/15287394.2020.1844827

Platanić Arizanović L, Nikolić-Kokić A, Brkljačić J, Tatalović N, Miler M, Oreščanin Dušić Z, Vidonja Uzelac T, Nikolić M, Milošević V, Blagojević D, Spasić S, Miljević Č. Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction. in Journal of Toxicology and Environmental Health, Part A. 2021;84(4):173-182.
doi:10.1080/15287394.2020.1844827 .

Platanić Arizanović, Lena, Nikolić-Kokić, Aleksandra, Brkljačić, Jelena, Tatalović, Nikola, Miler, Marko, Oreščanin Dušić, Zorana, Vidonja Uzelac, Teodora, Nikolić, Milan, Milošević, Verica, Blagojević, Duško, Spasić, Snežana, Miljević, Čedo, "Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction" in Journal of Toxicology and Environmental Health, Part A, 84, no. 4 (2021):173-182,
https://doi.org/10.1080/15287394.2020.1844827 . .

TY  - JOUR
AU  - Radić, Ivan
AU  - Mirić, Mirjana
AU  - Mijović, Milica
AU  - Tatalović, Nikola
AU  - Mitić, Miloš
AU  - Nestorović, Vojkan
AU  - Adžić, Miroslav
AU  - Blagojević, Duško
AU  - Popović, Ljiljana
AU  - Janićijević Hudomal, Snežana
PY  - 2021
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6170
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4181
AB  - Pumpkin seed oil (PSO) possesses a protective potential against liver injury due to the presence of biologically active ingredients. Adult male albino rats were administrated PSO (per os, 2 mL/kg b.w./day) and a 12% ethanol solution in water, ad libitum, with an average intake of 8.14 g of ethanol/kg bw/day for 6 weeks. Congestion, hepatic central vein dila-tion, portal vein branch dilation, Kupffer cell hyperplasia, fatty liver changes, hepatocyte focal necrosis were observed after daily alcohol intake. All observed changes were reduced when PSO was ingested with ethanol. PSO intake itself induced discrete cellular edema, congestion and slight dilatation of the central and portal vain branches. Chronic ethanol intake elevated catalase (CAT) activity and glutathione reductase (GR) protein expression; concomitant PSO intake had no effect on CAT activity or GR protein expression. PSO intake decreased the activities of GR, glutathione-S-transferase (GST) and xanthine oxidase (XOD) in the liver, probably due to the ingestion of antioxidants. Intake of PSO and ethanol significantly decreased cytosolic superoxide dismutase (SOD1) and increased NF-κB protein expression compared to ethanol intake, suggesting that the protective effects of PSO were mediated by the NF-κB signaling pathway. Our results reveal a therapeutic potential of PSO in alcoholic liver disease.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption
IS  - 1
VL  - 73
DO  - 10.2298/ABS201205008R
SP  - 123
EP  - 133
ER  - 

@article{
author = "Radić, Ivan and Mirić, Mirjana and Mijović, Milica and Tatalović, Nikola and Mitić, Miloš and Nestorović, Vojkan and Adžić, Miroslav and Blagojević, Duško and Popović, Ljiljana and Janićijević Hudomal, Snežana",
year = "2021",
abstract = "Pumpkin seed oil (PSO) possesses a protective potential against liver injury due to the presence of biologically active ingredients. Adult male albino rats were administrated PSO (per os, 2 mL/kg b.w./day) and a 12% ethanol solution in water, ad libitum, with an average intake of 8.14 g of ethanol/kg bw/day for 6 weeks. Congestion, hepatic central vein dila-tion, portal vein branch dilation, Kupffer cell hyperplasia, fatty liver changes, hepatocyte focal necrosis were observed after daily alcohol intake. All observed changes were reduced when PSO was ingested with ethanol. PSO intake itself induced discrete cellular edema, congestion and slight dilatation of the central and portal vain branches. Chronic ethanol intake elevated catalase (CAT) activity and glutathione reductase (GR) protein expression; concomitant PSO intake had no effect on CAT activity or GR protein expression. PSO intake decreased the activities of GR, glutathione-S-transferase (GST) and xanthine oxidase (XOD) in the liver, probably due to the ingestion of antioxidants. Intake of PSO and ethanol significantly decreased cytosolic superoxide dismutase (SOD1) and increased NF-κB protein expression compared to ethanol intake, suggesting that the protective effects of PSO were mediated by the NF-κB signaling pathway. Our results reveal a therapeutic potential of PSO in alcoholic liver disease.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption",
number = "1",
volume = "73",
doi = "10.2298/ABS201205008R",
pages = "123-133"
}

Radić, I., Mirić, M., Mijović, M., Tatalović, N., Mitić, M., Nestorović, V., Adžić, M., Blagojević, D., Popović, L.,& Janićijević Hudomal, S.. (2021). Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption. in Archives of Biological Sciences
Serbian Biological Society., 73(1), 123-133.
https://doi.org/10.2298/ABS201205008R

Radić I, Mirić M, Mijović M, Tatalović N, Mitić M, Nestorović V, Adžić M, Blagojević D, Popović L, Janićijević Hudomal S. Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption. in Archives of Biological Sciences. 2021;73(1):123-133.
doi:10.2298/ABS201205008R .

Radić, Ivan, Mirić, Mirjana, Mijović, Milica, Tatalović, Nikola, Mitić, Miloš, Nestorović, Vojkan, Adžić, Miroslav, Blagojević, Duško, Popović, Ljiljana, Janićijević Hudomal, Snežana, "Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption" in Archives of Biological Sciences, 73, no. 1 (2021):123-133,
https://doi.org/10.2298/ABS201205008R . .

TY  - JOUR
AU  - Kovačević, Sanja
AU  - Elaković, Ivana
AU  - Vojnović-Milutinović, Danijela
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Matić, Gordana
AU  - Tappy, Luc
AU  - Đorđević, Ana
AU  - Brkljačić, Jelena
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4468
AB  - Background: Both fructose consumption and chronic stress contribute to the development of metabolic disorders.
The consequences of such combination are not fully understood.
Objective: We investigated whether fructose supplementation and chronic stress synergistically disturb hepatic lipid
and glucose metabolism. The role of energy sensing, redox, and inflammatory status during development of metabolic
disturbances was investigated.
Methods: Female Wistar rats, aged 2.5 mo, were divided into 4 experimental groups: control (C) fed a standard diet
(commercial food and drinking water); fructose (F) fed the same food and 10% fructose solution; stress (S) fed the
standard diet and subjected to chronic unpredictable stress and, stress + fructose (SF) combining conditions F and
S as above. Stress included daily stressors: cold water forced swimming, physical restraint, cold room, wet bedding,
rocking, switching, or tilting cages. After 9 wk, hepatic enzymes and transcription factors involved in gluconeogenesis,
lipogenesis, fatty acid oxidation, antioxidative defence, energy sensing, and cytokines were assessed by qPCR, Western
blotting, and spectrophotometry and analyzed by 2-factor ANOVA.
Results: Fructose increased AMP-activated protein kinase (AMPK) phosphorylation (40%; P < 0.05) and the ratio of
inhibitory phosphorylation to total acetyl-CoA carboxylase (46%; P < 0.01), and decreased sterol regulatory element
binding protein 1c nuclear translocation by 30% (P < 0.05) in F and SF compared with C rats. Increased phosPck
(phoenolpyruvate carboxykinase) (85%) and G6pase (glucose-6-phosphatase) (55%) was observed in S rats (P < 0.05).
A 40% decrease in Apob (apolipoprotein B-100) and an increase in hepatic lipids (P < 0.05), together with a double
increase in TNF-α (P < 0.001), were observed in S rats, but without liver histopathological changes. These stress effects
on lipid accumulation and TNF-α were abolished in SF rats (P < 0.05).
Conclusions: Fructose does not enhance stress effects on hepatic lipid and glucose metabolism but attenuates its
effects on hepatic lipid accumulation and inflammation, suggesting that, in female rats, AMPK activation prevails over
stress-induced effects.
PB  - Oxford University Press on behalf of the American Society of Nutrition
T2  - The Journal of Nutrition
T1  - Fructose-Rich Diet Attenuates Stress-Induced Metabolic Disturbances in the Liver of Adult Female Rats
IS  - 12
VL  - 151
DO  - 10.1093/jn/nxab294
SP  - 3661
EP  - 3670
ER  - 

@article{
author = "Kovačević, Sanja and Elaković, Ivana and Vojnović-Milutinović, Danijela and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Matić, Gordana and Tappy, Luc and Đorđević, Ana and Brkljačić, Jelena",
year = "2021",
abstract = "Background: Both fructose consumption and chronic stress contribute to the development of metabolic disorders.
The consequences of such combination are not fully understood.
Objective: We investigated whether fructose supplementation and chronic stress synergistically disturb hepatic lipid
and glucose metabolism. The role of energy sensing, redox, and inflammatory status during development of metabolic
disturbances was investigated.
Methods: Female Wistar rats, aged 2.5 mo, were divided into 4 experimental groups: control (C) fed a standard diet
(commercial food and drinking water); fructose (F) fed the same food and 10% fructose solution; stress (S) fed the
standard diet and subjected to chronic unpredictable stress and, stress + fructose (SF) combining conditions F and
S as above. Stress included daily stressors: cold water forced swimming, physical restraint, cold room, wet bedding,
rocking, switching, or tilting cages. After 9 wk, hepatic enzymes and transcription factors involved in gluconeogenesis,
lipogenesis, fatty acid oxidation, antioxidative defence, energy sensing, and cytokines were assessed by qPCR, Western
blotting, and spectrophotometry and analyzed by 2-factor ANOVA.
Results: Fructose increased AMP-activated protein kinase (AMPK) phosphorylation (40%; P < 0.05) and the ratio of
inhibitory phosphorylation to total acetyl-CoA carboxylase (46%; P < 0.01), and decreased sterol regulatory element
binding protein 1c nuclear translocation by 30% (P < 0.05) in F and SF compared with C rats. Increased phosPck
(phoenolpyruvate carboxykinase) (85%) and G6pase (glucose-6-phosphatase) (55%) was observed in S rats (P < 0.05).
A 40% decrease in Apob (apolipoprotein B-100) and an increase in hepatic lipids (P < 0.05), together with a double
increase in TNF-α (P < 0.001), were observed in S rats, but without liver histopathological changes. These stress effects
on lipid accumulation and TNF-α were abolished in SF rats (P < 0.05).
Conclusions: Fructose does not enhance stress effects on hepatic lipid and glucose metabolism but attenuates its
effects on hepatic lipid accumulation and inflammation, suggesting that, in female rats, AMPK activation prevails over
stress-induced effects.",
publisher = "Oxford University Press on behalf of the American Society of Nutrition",
journal = "The Journal of Nutrition",
title = "Fructose-Rich Diet Attenuates Stress-Induced Metabolic Disturbances in the Liver of Adult Female Rats",
number = "12",
volume = "151",
doi = "10.1093/jn/nxab294",
pages = "3661-3670"
}

Kovačević, S., Elaković, I., Vojnović-Milutinović, D., Nikolić-Kokić, A., Blagojević, D., Matić, G., Tappy, L., Đorđević, A.,& Brkljačić, J.. (2021). Fructose-Rich Diet Attenuates Stress-Induced Metabolic Disturbances in the Liver of Adult Female Rats. in The Journal of Nutrition
Oxford University Press on behalf of the American Society of Nutrition., 151(12), 3661-3670.
https://doi.org/10.1093/jn/nxab294

Kovačević S, Elaković I, Vojnović-Milutinović D, Nikolić-Kokić A, Blagojević D, Matić G, Tappy L, Đorđević A, Brkljačić J. Fructose-Rich Diet Attenuates Stress-Induced Metabolic Disturbances in the Liver of Adult Female Rats. in The Journal of Nutrition. 2021;151(12):3661-3670.
doi:10.1093/jn/nxab294 .

Kovačević, Sanja, Elaković, Ivana, Vojnović-Milutinović, Danijela, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Matić, Gordana, Tappy, Luc, Đorđević, Ana, Brkljačić, Jelena, "Fructose-Rich Diet Attenuates Stress-Induced Metabolic Disturbances in the Liver of Adult Female Rats" in The Journal of Nutrition, 151, no. 12 (2021):3661-3670,
https://doi.org/10.1093/jn/nxab294 . .

TY  - JOUR
AU  - Tatalović, Nikola
AU  - Vidonja Uzelac, Teodora
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Bresjanac, Mara
AU  - Blagojević, Duško
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4662
AB  - Ibogaine effects are mediated by cellular receptors, ATP depletion followed by ROS
production and antioxidant enzyme activity elevation in a dose and time dependent manner. Since
the role of KATP channels and β -adrenoceptors in ROS cellular circuit was established here we
explored their role in ibogaine pro-antioxidant effectiveness. Single dose of ibogaine (10 mg/L i.e.,
28.8 µmol/L) was applied to isolated rat uterus (spontaneous and Ca2+-stimulated) and contractility
and antioxidant enzymes activity were monitored during 4 h. Ibogaine increased amplitude and
frequency of spontaneous active uteri immediately after addition that was prevented by propranolol
( 1 and  2 adrenoceptors selective antagonists) and glibenclamide (KATP sensitive channels inhibitor;
only frequency) pre-treatment. In Ca2+-stimulated uteri, ibogaine decreased both amplitude and
frequency after 4 h. Pre-treatment with propranolol abolished ibogaine induced amplitude lowering,
while glibenclamide had no effect. In both types of active uterus, ibogaine induced a decrease in
SOD1 and an increase in CAT activity after 2 h. In Ca2+-stimulated uterus, there was also a decrease
of SOD2 activity after 2 h. After 4 h, SOD1 activity returned to the baseline level, but GSH-Px activity
increased. Pre-treatment with both propranolol and glibenclamide abolished observed changes of
antioxidant enzymes activity suggesting that ibogaine pro-antioxidative effectiveness is β -adrenergic
receptors and KATP channels mediated.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated
IS  - 11
VL  - 10
DO  - 10.3390/antiox10111792
SP  - 1792
ER  - 

@article{
author = "Tatalović, Nikola and Vidonja Uzelac, Teodora and Oreščanin Dušić, Zorana and Nikolić-Kokić, Aleksandra and Bresjanac, Mara and Blagojević, Duško",
year = "2021",
abstract = "Ibogaine effects are mediated by cellular receptors, ATP depletion followed by ROS
production and antioxidant enzyme activity elevation in a dose and time dependent manner. Since
the role of KATP channels and β -adrenoceptors in ROS cellular circuit was established here we
explored their role in ibogaine pro-antioxidant effectiveness. Single dose of ibogaine (10 mg/L i.e.,
28.8 µmol/L) was applied to isolated rat uterus (spontaneous and Ca2+-stimulated) and contractility
and antioxidant enzymes activity were monitored during 4 h. Ibogaine increased amplitude and
frequency of spontaneous active uteri immediately after addition that was prevented by propranolol
( 1 and  2 adrenoceptors selective antagonists) and glibenclamide (KATP sensitive channels inhibitor;
only frequency) pre-treatment. In Ca2+-stimulated uteri, ibogaine decreased both amplitude and
frequency after 4 h. Pre-treatment with propranolol abolished ibogaine induced amplitude lowering,
while glibenclamide had no effect. In both types of active uterus, ibogaine induced a decrease in
SOD1 and an increase in CAT activity after 2 h. In Ca2+-stimulated uterus, there was also a decrease
of SOD2 activity after 2 h. After 4 h, SOD1 activity returned to the baseline level, but GSH-Px activity
increased. Pre-treatment with both propranolol and glibenclamide abolished observed changes of
antioxidant enzymes activity suggesting that ibogaine pro-antioxidative effectiveness is β -adrenergic
receptors and KATP channels mediated.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated",
number = "11",
volume = "10",
doi = "10.3390/antiox10111792",
pages = "1792"
}

Tatalović, N., Vidonja Uzelac, T., Oreščanin Dušić, Z., Nikolić-Kokić, A., Bresjanac, M.,& Blagojević, D.. (2021). Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated. in Antioxidants
Basel: MDPI., 10(11), 1792.
https://doi.org/10.3390/antiox10111792

Tatalović N, Vidonja Uzelac T, Oreščanin Dušić Z, Nikolić-Kokić A, Bresjanac M, Blagojević D. Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated. in Antioxidants. 2021;10(11):1792.
doi:10.3390/antiox10111792 .

Tatalović, Nikola, Vidonja Uzelac, Teodora, Oreščanin Dušić, Zorana, Nikolić-Kokić, Aleksandra, Bresjanac, Mara, Blagojević, Duško, "Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated" in Antioxidants, 10, no. 11 (2021):1792,
https://doi.org/10.3390/antiox10111792 . .

TY  - JOUR
AU  - Popović, Željko D.
AU  - Maier, Vítězslav
AU  - Avramov, Miloš
AU  - Uzelac, Iva
AU  - Gošić-Dondo, Snežana
AU  - Blagojević, Duško
AU  - Koštál, Vladimír
PY  - 2021
UR  - https://www.frontiersin.org/articles/10.3389/fphys.2021.768593/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4689
AB  - The European corn borer Ostrinia nubilalis is a pest species, whose fifth instar larvae gradually develop cold hardiness during diapause. The physiological changes underlying diapause progression and cold hardiness development are still insufficiently understood in insects. Here, we follow a complex of changes related to energy metabolism during cold acclimation (5°C) of diapausing larvae and compare this to warm-acclimated (22°C) and non-diapause controls. Capillary electrophoresis of nucleotides and coenzymes has shown that in gradually cold-acclimated groups concentrations of ATP/ADP and, consequently, energy charge slowly decrease during diapause, while the concentration of AMP increases, especially in the first months of diapause. Also, the activity of cytochrome c oxidase (COX), as well as the concentrations of NAD + and GMP, decline in cold-acclimated groups, until the latter part of diapause, when they recover. Relative expression of NADH dehydrogenase ( nd1 ), coenzyme Q-cytochrome c reductase ( uqcr ), COX, ATP synthase ( atp ), ADP/ATP translocase ( ant ), and prohibitin 2 ( phb2 ) is supressed in cold-acclimated larvae during the first months of diapause and gradually increases toward the termination of diapause. Contrary to this, NADP + and UMP levels significantly increased in the first few months of diapause, after gradual cold acclimation, which is in connection with the biosynthesis of cryoprotective molecules, as well as regeneration of small antioxidants. Our findings evidence the existence of a cold-induced energy-saving program that facilitates long-term maintenance of larval diapause, as well as gradual development of cold hardiness. In contrast, warm acclimation induced faster depletion of ATP, ADP, UMP, NAD + , and NADP + , as well as higher activity of COX and generally higher expression of all energy-related genes in comparison to cold-acclimated larvae. Moreover, such unusually high metabolic activity, driven by high temperatures, lead to premature mortality in the warm-acclimated group after 2 months of diapause. Thus, our findings strongly support the importance of low temperature exposure in early diapause for gradual cold hardiness acquisition, successful maintenance of the resting state and return to active development. Moreover, they demonstrate potentially adverse effects of global climate changes and subsequent increase in winter temperatures on cold-adapted terrestrial organisms in temperate and subpolar regions.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Physiology
T1  - Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.)
VL  - 12
DO  - 10.3389/fphys.2021.768593
SP  - 768593
ER  - 

@article{
author = "Popović, Željko D. and Maier, Vítězslav and Avramov, Miloš and Uzelac, Iva and Gošić-Dondo, Snežana and Blagojević, Duško and Koštál, Vladimír",
year = "2021",
abstract = "The European corn borer Ostrinia nubilalis is a pest species, whose fifth instar larvae gradually develop cold hardiness during diapause. The physiological changes underlying diapause progression and cold hardiness development are still insufficiently understood in insects. Here, we follow a complex of changes related to energy metabolism during cold acclimation (5°C) of diapausing larvae and compare this to warm-acclimated (22°C) and non-diapause controls. Capillary electrophoresis of nucleotides and coenzymes has shown that in gradually cold-acclimated groups concentrations of ATP/ADP and, consequently, energy charge slowly decrease during diapause, while the concentration of AMP increases, especially in the first months of diapause. Also, the activity of cytochrome c oxidase (COX), as well as the concentrations of NAD + and GMP, decline in cold-acclimated groups, until the latter part of diapause, when they recover. Relative expression of NADH dehydrogenase ( nd1 ), coenzyme Q-cytochrome c reductase ( uqcr ), COX, ATP synthase ( atp ), ADP/ATP translocase ( ant ), and prohibitin 2 ( phb2 ) is supressed in cold-acclimated larvae during the first months of diapause and gradually increases toward the termination of diapause. Contrary to this, NADP + and UMP levels significantly increased in the first few months of diapause, after gradual cold acclimation, which is in connection with the biosynthesis of cryoprotective molecules, as well as regeneration of small antioxidants. Our findings evidence the existence of a cold-induced energy-saving program that facilitates long-term maintenance of larval diapause, as well as gradual development of cold hardiness. In contrast, warm acclimation induced faster depletion of ATP, ADP, UMP, NAD + , and NADP + , as well as higher activity of COX and generally higher expression of all energy-related genes in comparison to cold-acclimated larvae. Moreover, such unusually high metabolic activity, driven by high temperatures, lead to premature mortality in the warm-acclimated group after 2 months of diapause. Thus, our findings strongly support the importance of low temperature exposure in early diapause for gradual cold hardiness acquisition, successful maintenance of the resting state and return to active development. Moreover, they demonstrate potentially adverse effects of global climate changes and subsequent increase in winter temperatures on cold-adapted terrestrial organisms in temperate and subpolar regions.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Physiology",
title = "Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.)",
volume = "12",
doi = "10.3389/fphys.2021.768593",
pages = "768593"
}

Popović, Ž. D., Maier, V., Avramov, M., Uzelac, I., Gošić-Dondo, S., Blagojević, D.,& Koštál, V.. (2021). Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.). in Frontiers in Physiology
Lausanne: Frontiers Media S.A.., 12, 768593.
https://doi.org/10.3389/fphys.2021.768593

Popović ŽD, Maier V, Avramov M, Uzelac I, Gošić-Dondo S, Blagojević D, Koštál V. Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.). in Frontiers in Physiology. 2021;12:768593.
doi:10.3389/fphys.2021.768593 .

Popović, Željko D., Maier, Vítězslav, Avramov, Miloš, Uzelac, Iva, Gošić-Dondo, Snežana, Blagojević, Duško, Koštál, Vladimír, "Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.)" in Frontiers in Physiology, 12 (2021):768593,
https://doi.org/10.3389/fphys.2021.768593 . .

TY  - JOUR
AU  - Tatalović, Nikola
AU  - Vidonja Uzelac, Teodora
AU  - Mijović, Milica
AU  - Koželj, Gordana
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Bresjanac, Mara
AU  - Blagojević, Duško
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4706
AB  - Ibogaine induces rapid changes in cellular energetics followed by the elevation of antioxidant activities. As shown earlier in male rats, ibogaine treatment with both 1 and 20 mg/kg b.w. per os led to significant glycogenolytic activity in the liver. In this work, female rats treated with the same doses of ibogaine per os displayed lower liver glycogenolytic activity relative to males, dilatation of the central vein and branches of the portal vein, and increased concentration of thiols 6 h after treatment. These changes were followed by increased catalase activity and lipid peroxidation, and decreased xanthine oxidase activity after 24 h. In kidneys, mild histopathological changes were found in all treated animals, accompanied by a decrease of glutathione reductase (after 6 and 24 h at both doses) and an increase of catalase (6 h) and xanthine oxidase activity (6 and 24 h). Ibogaine did not affect antioxidant enzymes activity in erythrocytes. Bioavailability of ibogaine was two to three times higher in females than males, with similar kinetic profiles. Compared to previous results in males, ibogaine showed sex specific effect at the level of antioxidant cellular system. Effects of ibogaine in rats are sex- and tissue-specific, and also dose- and time-dependent.
PB  - Basel: MDPI
T2  - Life
T1  - Ibogaine Has Sex-Specific Plasma Bioavailability, Histopathological and Redox/Antioxidant Effects in Rat Liver and Kidneys: A Study on Females
IS  - 1
VL  - 12
DO  - 10.3390/life12010016
SP  - 16
ER  - 

@article{
author = "Tatalović, Nikola and Vidonja Uzelac, Teodora and Mijović, Milica and Koželj, Gordana and Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Bresjanac, Mara and Blagojević, Duško",
year = "2021",
abstract = "Ibogaine induces rapid changes in cellular energetics followed by the elevation of antioxidant activities. As shown earlier in male rats, ibogaine treatment with both 1 and 20 mg/kg b.w. per os led to significant glycogenolytic activity in the liver. In this work, female rats treated with the same doses of ibogaine per os displayed lower liver glycogenolytic activity relative to males, dilatation of the central vein and branches of the portal vein, and increased concentration of thiols 6 h after treatment. These changes were followed by increased catalase activity and lipid peroxidation, and decreased xanthine oxidase activity after 24 h. In kidneys, mild histopathological changes were found in all treated animals, accompanied by a decrease of glutathione reductase (after 6 and 24 h at both doses) and an increase of catalase (6 h) and xanthine oxidase activity (6 and 24 h). Ibogaine did not affect antioxidant enzymes activity in erythrocytes. Bioavailability of ibogaine was two to three times higher in females than males, with similar kinetic profiles. Compared to previous results in males, ibogaine showed sex specific effect at the level of antioxidant cellular system. Effects of ibogaine in rats are sex- and tissue-specific, and also dose- and time-dependent.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Ibogaine Has Sex-Specific Plasma Bioavailability, Histopathological and Redox/Antioxidant Effects in Rat Liver and Kidneys: A Study on Females",
number = "1",
volume = "12",
doi = "10.3390/life12010016",
pages = "16"
}

Tatalović, N., Vidonja Uzelac, T., Mijović, M., Koželj, G., Nikolić-Kokić, A., Oreščanin Dušić, Z., Bresjanac, M.,& Blagojević, D.. (2021). Ibogaine Has Sex-Specific Plasma Bioavailability, Histopathological and Redox/Antioxidant Effects in Rat Liver and Kidneys: A Study on Females. in Life
Basel: MDPI., 12(1), 16.
https://doi.org/10.3390/life12010016

Tatalović N, Vidonja Uzelac T, Mijović M, Koželj G, Nikolić-Kokić A, Oreščanin Dušić Z, Bresjanac M, Blagojević D. Ibogaine Has Sex-Specific Plasma Bioavailability, Histopathological and Redox/Antioxidant Effects in Rat Liver and Kidneys: A Study on Females. in Life. 2021;12(1):16.
doi:10.3390/life12010016 .

Tatalović, Nikola, Vidonja Uzelac, Teodora, Mijović, Milica, Koželj, Gordana, Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Bresjanac, Mara, Blagojević, Duško, "Ibogaine Has Sex-Specific Plasma Bioavailability, Histopathological and Redox/Antioxidant Effects in Rat Liver and Kidneys: A Study on Females" in Life, 12, no. 1 (2021):16,
https://doi.org/10.3390/life12010016 . .

TY  - JOUR
AU  - Vukašinović, Elvira
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Čelić, Tatjana
AU  - Pihler, Ivan
AU  - Blagojević, Duško
PY  - 2021
UR  - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100417&tlng=en
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4699
AB  - The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and trans1. Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. Brazilian Arch Biol Technol. 2021;64:e21200773. cription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action.
PB  - Parana, Brazil: Technology Institute of Paraná
T2  - Brazilian Archives of Biology and Technology
T1  - Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut
VL  - 64
DO  - 10.1590/1678-4324-2021200773
SP  - e21200773
ER  - 

@article{
author = "Vukašinović, Elvira and Purać, Jelena and Kojić, Danijela and Čelić, Tatjana and Pihler, Ivan and Blagojević, Duško",
year = "2021",
abstract = "The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and trans1. Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. Brazilian Arch Biol Technol. 2021;64:e21200773. cription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action.",
publisher = "Parana, Brazil: Technology Institute of Paraná",
journal = "Brazilian Archives of Biology and Technology",
title = "Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut",
volume = "64",
doi = "10.1590/1678-4324-2021200773",
pages = "e21200773"
}

Vukašinović, E., Purać, J., Kojić, D., Čelić, T., Pihler, I.,& Blagojević, D.. (2021). Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. in Brazilian Archives of Biology and Technology
Parana, Brazil: Technology Institute of Paraná., 64, e21200773.
https://doi.org/10.1590/1678-4324-2021200773

Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. in Brazilian Archives of Biology and Technology. 2021;64:e21200773.
doi:10.1590/1678-4324-2021200773 .

Vukašinović, Elvira, Purać, Jelena, Kojić, Danijela, Čelić, Tatjana, Pihler, Ivan, Blagojević, Duško, "Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut" in Brazilian Archives of Biology and Technology, 64 (2021):e21200773,
https://doi.org/10.1590/1678-4324-2021200773 . .

TY  - CONF
AU  - Guševac Stojanović, Ivana
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Tatalović, Nikola
AU  - Blagojević, Duško
AU  - Martinović, Jelena
AU  - Grković, Ivana
AU  - Mitrović, Nataša
AU  - Zarić, Marina
AU  - Drakulić, Dunja
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5247
AB  - Numerous natural compounds, like progesterone (P4), a sex steroid hormone, are highlighted as promising agents for treatment of different disorders including prolonged disturbance of blood flow. However, its action on several oxidative stress markers (pro/antioxidant balance, products of lipid peroxidation and phosphatidylcholine to lysophosphatidylcholine intensity ratio) and one of the major components of antioxidant system, superoxide dismutase in rat prefrontal cortex (PFC) following permanent bilateral occlusion of common carotid arteries (2VO) is not completely investigated. According to the obtained results, levels of investigated oxidative stress markers and SOD activity were altered in 2VO animals treated with vehicle, while P4 treatment returned them to control values. Overall, presented data indicate that P4 might manifest antioxidative features in PFC of 2VO rats.
PB  - Belgrade: Society of Physical Chemists of Serbia
C3  - Proceeding: 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2021: the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction. Vol. 1; 2021 Sep 20-24; Belgrade, Serbia
T1  - Cerebral hypoperfusion and progesterone treatment alter parameters of oxidative stress and antioxidant defence in male rats
VL  - 1
SP  - 25
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5247
ER  - 

@conference{
author = "Guševac Stojanović, Ivana and Todorović, Ana and Pejić, Snežana and Tatalović, Nikola and Blagojević, Duško and Martinović, Jelena and Grković, Ivana and Mitrović, Nataša and Zarić, Marina and Drakulić, Dunja",
year = "2021",
abstract = "Numerous natural compounds, like progesterone (P4), a sex steroid hormone, are highlighted as promising agents for treatment of different disorders including prolonged disturbance of blood flow. However, its action on several oxidative stress markers (pro/antioxidant balance, products of lipid peroxidation and phosphatidylcholine to lysophosphatidylcholine intensity ratio) and one of the major components of antioxidant system, superoxide dismutase in rat prefrontal cortex (PFC) following permanent bilateral occlusion of common carotid arteries (2VO) is not completely investigated. According to the obtained results, levels of investigated oxidative stress markers and SOD activity were altered in 2VO animals treated with vehicle, while P4 treatment returned them to control values. Overall, presented data indicate that P4 might manifest antioxidative features in PFC of 2VO rats.",
publisher = "Belgrade: Society of Physical Chemists of Serbia",
journal = "Proceeding: 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2021: the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction. Vol. 1; 2021 Sep 20-24; Belgrade, Serbia",
title = "Cerebral hypoperfusion and progesterone treatment alter parameters of oxidative stress and antioxidant defence in male rats",
volume = "1",
pages = "25-32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5247"
}

Guševac Stojanović, I., Todorović, A., Pejić, S., Tatalović, N., Blagojević, D., Martinović, J., Grković, I., Mitrović, N., Zarić, M.,& Drakulić, D.. (2021). Cerebral hypoperfusion and progesterone treatment alter parameters of oxidative stress and antioxidant defence in male rats. in Proceeding: 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2021: the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction. Vol. 1; 2021 Sep 20-24; Belgrade, Serbia
Belgrade: Society of Physical Chemists of Serbia., 1, 25-32.
https://hdl.handle.net/21.15107/rcub_ibiss_5247

Guševac Stojanović I, Todorović A, Pejić S, Tatalović N, Blagojević D, Martinović J, Grković I, Mitrović N, Zarić M, Drakulić D. Cerebral hypoperfusion and progesterone treatment alter parameters of oxidative stress and antioxidant defence in male rats. in Proceeding: 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2021: the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction. Vol. 1; 2021 Sep 20-24; Belgrade, Serbia. 2021;1:25-32.
https://hdl.handle.net/21.15107/rcub_ibiss_5247 .

Guševac Stojanović, Ivana, Todorović, Ana, Pejić, Snežana, Tatalović, Nikola, Blagojević, Duško, Martinović, Jelena, Grković, Ivana, Mitrović, Nataša, Zarić, Marina, Drakulić, Dunja, "Cerebral hypoperfusion and progesterone treatment alter parameters of oxidative stress and antioxidant defence in male rats" in Proceeding: 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2021: the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction. Vol. 1; 2021 Sep 20-24; Belgrade, Serbia, 1 (2021):25-32,
https://hdl.handle.net/21.15107/rcub_ibiss_5247 .

TY  - JOUR
AU  - Ilijević, Konstantin
AU  - Vujanović, Dragana
AU  - Orčić, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Zarić, Nenad
AU  - Gržetić, Ivan
AU  - Blagojević, Duško
AU  - Čelić, Tatjana V.
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32777467
UR  - https://radar.ibiss.bg.ac.rs/123456789/3861
AB  - Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
T1  - Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.
VL  - 239
DO  - 10.1016/j.cbpc.2020.108852
SP  - 108852
ER  - 

@article{
author = "Ilijević, Konstantin and Vujanović, Dragana and Orčić, Snežana and Purać, Jelena and Kojić, Danijela and Zarić, Nenad and Gržetić, Ivan and Blagojević, Duško and Čelić, Tatjana V.",
year = "2021",
abstract = "Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
title = "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.",
volume = "239",
doi = "10.1016/j.cbpc.2020.108852",
pages = "108852"
}

Ilijević, K., Vujanović, D., Orčić, S., Purać, J., Kojić, D., Zarić, N., Gržetić, I., Blagojević, D.,& Čelić, T. V.. (2021). Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Elsevier Inc.., 239, 108852.
https://doi.org/10.1016/j.cbpc.2020.108852

Ilijević K, Vujanović D, Orčić S, Purać J, Kojić D, Zarić N, Gržetić I, Blagojević D, Čelić TV. Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2021;239:108852.
doi:10.1016/j.cbpc.2020.108852 .

Ilijević, Konstantin, Vujanović, Dragana, Orčić, Snežana, Purać, Jelena, Kojić, Danijela, Zarić, Nenad, Gržetić, Ivan, Blagojević, Duško, Čelić, Tatjana V., "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph." in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 239 (2021):108852,
https://doi.org/10.1016/j.cbpc.2020.108852 . .

TY  - DATA
AU  - Radić, Ivan
AU  - Mirić, Mirjana
AU  - Mijović, Milica
AU  - Tatalović, Nikola
AU  - Mitić, Miloš
AU  - Nestorović, Vojkan
AU  - Adžić, Miroslav
AU  - Blagojević, Duško
AU  - Popović, Ljiljana
AU  - Janićijević Hudomal, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4182
AB  - Supplementary Fig.S1. Scheme of the experimental design; Supplementary TableS2. Chemical analysis of commercialcold pressedpumpkin seed oil that was used in theexperiment.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Supplementary data for the article: Radic I, Miric M, Mijovic M, Tatalovic N, Mitic M, Nestorovic V, Adzic M, Blagojevic D, Popovic L, Janicijevic-Hudomal S. Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption. Arch Biol Sci. 2021;73(1):123–33.
IS  - 1
VL  - 73
SP  - 123
EP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4182
ER  - 

@misc{
author = "Radić, Ivan and Mirić, Mirjana and Mijović, Milica and Tatalović, Nikola and Mitić, Miloš and Nestorović, Vojkan and Adžić, Miroslav and Blagojević, Duško and Popović, Ljiljana and Janićijević Hudomal, Snežana",
year = "2021",
abstract = "Supplementary Fig.S1. Scheme of the experimental design; Supplementary TableS2. Chemical analysis of commercialcold pressedpumpkin seed oil that was used in theexperiment.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Supplementary data for the article: Radic I, Miric M, Mijovic M, Tatalovic N, Mitic M, Nestorovic V, Adzic M, Blagojevic D, Popovic L, Janicijevic-Hudomal S. Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption. Arch Biol Sci. 2021;73(1):123–33.",
number = "1",
volume = "73",
pages = "123-133",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4182"
}

Radić, I., Mirić, M., Mijović, M., Tatalović, N., Mitić, M., Nestorović, V., Adžić, M., Blagojević, D., Popović, L.,& Janićijević Hudomal, S.. (2021). Supplementary data for the article: Radic I, Miric M, Mijovic M, Tatalovic N, Mitic M, Nestorovic V, Adzic M, Blagojevic D, Popovic L, Janicijevic-Hudomal S. Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption. Arch Biol Sci. 2021;73(1):123–33.. in Archives of Biological Sciences
Serbian Biological Society., 73(1), 123-133.
https://hdl.handle.net/21.15107/rcub_ibiss_4182

Radić I, Mirić M, Mijović M, Tatalović N, Mitić M, Nestorović V, Adžić M, Blagojević D, Popović L, Janićijević Hudomal S. Supplementary data for the article: Radic I, Miric M, Mijovic M, Tatalovic N, Mitic M, Nestorovic V, Adzic M, Blagojevic D, Popovic L, Janicijevic-Hudomal S. Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption. Arch Biol Sci. 2021;73(1):123–33.. in Archives of Biological Sciences. 2021;73(1):123-133.
https://hdl.handle.net/21.15107/rcub_ibiss_4182 .

Radić, Ivan, Mirić, Mirjana, Mijović, Milica, Tatalović, Nikola, Mitić, Miloš, Nestorović, Vojkan, Adžić, Miroslav, Blagojević, Duško, Popović, Ljiljana, Janićijević Hudomal, Snežana, "Supplementary data for the article: Radic I, Miric M, Mijovic M, Tatalovic N, Mitic M, Nestorovic V, Adzic M, Blagojevic D, Popovic L, Janicijevic-Hudomal S. Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption. Arch Biol Sci. 2021;73(1):123–33." in Archives of Biological Sciences, 73, no. 1 (2021):123-133,
https://hdl.handle.net/21.15107/rcub_ibiss_4182 .

TY  - JOUR
AU  - Spasić, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Miletić, Srđan
AU  - Oreščanin Dušić, Zorana
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
AU  - Stević, Zorica
PY  - 2020
UR  - https://www.eurekaselect.com/179582/article
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32077821
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3757
AB  - Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.
PB  - Bentham Science Publishers Ltd.
T2  - Current Drug Targets
T1  - Edaravone May Prevent Ferroptosis in ALS.
IS  - 8
VL  - 21
DO  - 10.2174/1389450121666200220123305
SP  - 776
EP  - 780
ER  - 

@article{
author = "Spasić, Snežana and Nikolić-Kokić, Aleksandra and Miletić, Srđan and Oreščanin Dušić, Zorana and Spasić, Mihajlo and Blagojević, Duško and Stević, Zorica",
year = "2020",
abstract = "Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.",
publisher = "Bentham Science Publishers Ltd.",
journal = "Current Drug Targets",
title = "Edaravone May Prevent Ferroptosis in ALS.",
number = "8",
volume = "21",
doi = "10.2174/1389450121666200220123305",
pages = "776-780"
}

Spasić, S., Nikolić-Kokić, A., Miletić, S., Oreščanin Dušić, Z., Spasić, M., Blagojević, D.,& Stević, Z.. (2020). Edaravone May Prevent Ferroptosis in ALS.. in Current Drug Targets
Bentham Science Publishers Ltd.., 21(8), 776-780.
https://doi.org/10.2174/1389450121666200220123305

Spasić S, Nikolić-Kokić A, Miletić S, Oreščanin Dušić Z, Spasić M, Blagojević D, Stević Z. Edaravone May Prevent Ferroptosis in ALS.. in Current Drug Targets. 2020;21(8):776-780.
doi:10.2174/1389450121666200220123305 .

Spasić, Snežana, Nikolić-Kokić, Aleksandra, Miletić, Srđan, Oreščanin Dušić, Zorana, Spasić, Mihajlo, Blagojević, Duško, Stević, Zorica, "Edaravone May Prevent Ferroptosis in ALS." in Current Drug Targets, 21, no. 8 (2020):776-780,
https://doi.org/10.2174/1389450121666200220123305 . .

TY  - JOUR
AU  - Uzelac, Iva
AU  - Avramov, Miloš
AU  - Čelić, Tatjana
AU  - Vukašinović, Elvira
AU  - Gošić-Dondo, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Blagojević, Duško
AU  - Popović, Željko D.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3706
AB  - The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes – citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.
PB  - Nature Research
T2  - Scientific Reports
T1  - Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-65926-w
SP  - 1
EP  - 11
ER  - 

@article{
author = "Uzelac, Iva and Avramov, Miloš and Čelić, Tatjana and Vukašinović, Elvira and Gošić-Dondo, Snežana and Purać, Jelena and Kojić, Danijela and Blagojević, Duško and Popović, Željko D.",
year = "2020",
abstract = "The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes – citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-65926-w",
pages = "1-11"
}

Uzelac, I., Avramov, M., Čelić, T., Vukašinović, E., Gošić-Dondo, S., Purać, J., Kojić, D., Blagojević, D.,& Popović, Ž. D.. (2020). Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.). in Scientific Reports
Nature Research., 10(1), 1-11.
https://doi.org/10.1038/s41598-020-65926-w

Uzelac I, Avramov M, Čelić T, Vukašinović E, Gošić-Dondo S, Purać J, Kojić D, Blagojević D, Popović ŽD. Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.). in Scientific Reports. 2020;10(1):1-11.
doi:10.1038/s41598-020-65926-w .

Uzelac, Iva, Avramov, Miloš, Čelić, Tatjana, Vukašinović, Elvira, Gošić-Dondo, Snežana, Purać, Jelena, Kojić, Danijela, Blagojević, Duško, Popović, Željko D., "Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)" in Scientific Reports, 10, no. 1 (2020):1-11,
https://doi.org/10.1038/s41598-020-65926-w . .