TY  - JOUR
AU  - Vieira, Dalila M.
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Barros, Lillian
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Barreiro, Maria Filomena
AU  - Ferreira, Isabel C.F.R.
AU  - Castro, Maria Cidalia R.
AU  - Rodrigues, Pedro V.
AU  - Machado, Ana Vera
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/fft2.141
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5435
AB  - Natural extracts have been used in several traditional medicine applications and culinary purposes. Their biological properties (antioxidant and antimicrobial) are due to the presence of several active aromatic compounds. Herein, different natural extracts were evaluated, namely structural and thermal characterization and biological activity, in its natural form and incorporated into a polymeric matrix, to assess their effective potential as additives for active food packaging. While rosemary presented the highest thermal stability with a degradation starting at 327°C, lemon balm extract was the less stable (180°C). Regarding the thiobarbituric acid assay, all extracts presented antioxidant activity, in oxidative hemolysis inhibition; anise, cinnamon, and clove extract did not present any action. Overall, the results demonstrated that leaves (rosemary and green tea) and the rhizome (curcumin) are the plant parts with the best performance. Therefore, extracts from aromatic plants are promising natural additives that can be incorporated into polymeric matrices to produce active food packaging film, increasing products shelf-life.
PB  - Hoboken: Wiley
T2  - Food Frontiers
T1  - Evaluation of plant extracts as an efficient source of additives for active food packaging
IS  - 3
VL  - 3
DO  - 10.1002/fft2.141
SP  - 480
EP  - 488
ER  - 

@article{
author = "Vieira, Dalila M. and Pereira, Carla and Calhelha, Ricardo C. and Barros, Lillian and Petrović, Jovana and Soković, Marina and Barreiro, Maria Filomena and Ferreira, Isabel C.F.R. and Castro, Maria Cidalia R. and Rodrigues, Pedro V. and Machado, Ana Vera",
year = "2022",
abstract = "Natural extracts have been used in several traditional medicine applications and culinary purposes. Their biological properties (antioxidant and antimicrobial) are due to the presence of several active aromatic compounds. Herein, different natural extracts were evaluated, namely structural and thermal characterization and biological activity, in its natural form and incorporated into a polymeric matrix, to assess their effective potential as additives for active food packaging. While rosemary presented the highest thermal stability with a degradation starting at 327°C, lemon balm extract was the less stable (180°C). Regarding the thiobarbituric acid assay, all extracts presented antioxidant activity, in oxidative hemolysis inhibition; anise, cinnamon, and clove extract did not present any action. Overall, the results demonstrated that leaves (rosemary and green tea) and the rhizome (curcumin) are the plant parts with the best performance. Therefore, extracts from aromatic plants are promising natural additives that can be incorporated into polymeric matrices to produce active food packaging film, increasing products shelf-life.",
publisher = "Hoboken: Wiley",
journal = "Food Frontiers",
title = "Evaluation of plant extracts as an efficient source of additives for active food packaging",
number = "3",
volume = "3",
doi = "10.1002/fft2.141",
pages = "480-488"
}

Vieira, D. M., Pereira, C., Calhelha, R. C., Barros, L., Petrović, J., Soković, M., Barreiro, M. F., Ferreira, I. C.F.R., Castro, M. C. R., Rodrigues, P. V.,& Machado, A. V.. (2022). Evaluation of plant extracts as an efficient source of additives for active food packaging. in Food Frontiers
Hoboken: Wiley., 3(3), 480-488.
https://doi.org/10.1002/fft2.141

Vieira DM, Pereira C, Calhelha RC, Barros L, Petrović J, Soković M, Barreiro MF, Ferreira IC, Castro MCR, Rodrigues PV, Machado AV. Evaluation of plant extracts as an efficient source of additives for active food packaging. in Food Frontiers. 2022;3(3):480-488.
doi:10.1002/fft2.141 .

Vieira, Dalila M., Pereira, Carla, Calhelha, Ricardo C., Barros, Lillian, Petrović, Jovana, Soković, Marina, Barreiro, Maria Filomena, Ferreira, Isabel C.F.R., Castro, Maria Cidalia R., Rodrigues, Pedro V., Machado, Ana Vera, "Evaluation of plant extracts as an efficient source of additives for active food packaging" in Food Frontiers, 3, no. 3 (2022):480-488,
https://doi.org/10.1002/fft2.141 . .

TY  - JOUR
AU  - Othman, Souha
AU  - Añibarro-Ortega, Mikel
AU  - Dias, Maria Inês
AU  - Ćirić, Ana
AU  - Mandim, Filipa
AU  - Soković, Marina
AU  - Ferreira, Isabel C F R
AU  - Pinela, José
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9587281
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5175
AB  - Quince (Cydonia oblonga Mill.) is an astringent fruit widely processed into marmalade and other sweets through processes that discard the peel as a by-product. Therefore, this study was performed to characterize the quince peel composition in nutrients and phytochemicals and evaluate its in vitro biological activity, following a "zero waste" approach. The quince peel dry powder was particularly rich in fiber (20.2 g/100 g), fructose (34 g/100 g), malic acid (7.2 g/100 g), and potassium (692 mg/100 g). Extracts prepared by dynamic hydroethanolic maceration and hot water extraction yielded 4.70 and 4.27 mg/g of phenolic compounds, respectively, with a prevalence of flavan-3-ols. The hydroethanolic extract was the most effective in inhibiting lipid peroxidation and oxidative hemolysis, and also presented better antimicrobial effects against foodborne pathogens, which agreed with the highest flavan-3-ol contents. The extracts were better than control synthetic food additives against some tested fungal and bacterial strains. On the other hand, no ability to inhibit nitric oxide production or toxicity to the tumor and non-tumor cell lines was observed. Furthermore, the solid residues remaining after extraction contained 35-37 g/100 g of fiber. Overall, quince peel can be upcycled into fiber-rich and bioactive ingredients to endow the value chain with natural food fortifiers, preservatives, and health promoters.
PB  - Elsevier Ltd.
T2  - Heliyon
T1  - Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems
IS  - 10
VL  - 8
DO  - 10.1016/j.heliyon.2022.e11042
SP  - e11042
ER  - 

@article{
author = "Othman, Souha and Añibarro-Ortega, Mikel and Dias, Maria Inês and Ćirić, Ana and Mandim, Filipa and Soković, Marina and Ferreira, Isabel C F R and Pinela, José and Barros, Lillian",
year = "2022",
abstract = "Quince (Cydonia oblonga Mill.) is an astringent fruit widely processed into marmalade and other sweets through processes that discard the peel as a by-product. Therefore, this study was performed to characterize the quince peel composition in nutrients and phytochemicals and evaluate its in vitro biological activity, following a "zero waste" approach. The quince peel dry powder was particularly rich in fiber (20.2 g/100 g), fructose (34 g/100 g), malic acid (7.2 g/100 g), and potassium (692 mg/100 g). Extracts prepared by dynamic hydroethanolic maceration and hot water extraction yielded 4.70 and 4.27 mg/g of phenolic compounds, respectively, with a prevalence of flavan-3-ols. The hydroethanolic extract was the most effective in inhibiting lipid peroxidation and oxidative hemolysis, and also presented better antimicrobial effects against foodborne pathogens, which agreed with the highest flavan-3-ol contents. The extracts were better than control synthetic food additives against some tested fungal and bacterial strains. On the other hand, no ability to inhibit nitric oxide production or toxicity to the tumor and non-tumor cell lines was observed. Furthermore, the solid residues remaining after extraction contained 35-37 g/100 g of fiber. Overall, quince peel can be upcycled into fiber-rich and bioactive ingredients to endow the value chain with natural food fortifiers, preservatives, and health promoters.",
publisher = "Elsevier Ltd.",
journal = "Heliyon",
title = "Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems",
number = "10",
volume = "8",
doi = "10.1016/j.heliyon.2022.e11042",
pages = "e11042"
}

Othman, S., Añibarro-Ortega, M., Dias, M. I., Ćirić, A., Mandim, F., Soković, M., Ferreira, I. C. F. R., Pinela, J.,& Barros, L.. (2022). Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems. in Heliyon
Elsevier Ltd.., 8(10), e11042.
https://doi.org/10.1016/j.heliyon.2022.e11042

Othman S, Añibarro-Ortega M, Dias MI, Ćirić A, Mandim F, Soković M, Ferreira ICFR, Pinela J, Barros L. Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems. in Heliyon. 2022;8(10):e11042.
doi:10.1016/j.heliyon.2022.e11042 .

Othman, Souha, Añibarro-Ortega, Mikel, Dias, Maria Inês, Ćirić, Ana, Mandim, Filipa, Soković, Marina, Ferreira, Isabel C F R, Pinela, José, Barros, Lillian, "Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems" in Heliyon, 8, no. 10 (2022):e11042,
https://doi.org/10.1016/j.heliyon.2022.e11042 . .

TY  - JOUR
AU  - Silva, Ana Rita
AU  - Ayuso, Manuel
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - García, Pablo A.
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669021011080
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4746
AB  - Cytinus hypocistis (L.) L. is a comestible holoparasite with great potential for cosmeceutical application. Although its high tannin content has been associated with its bioactive and inhibitory enzyme properties, this is the first report establishing a relationship between parasite and host (Halimium lasianthum (Lam.) Greuter) phenolic profile and bioactive properties. Thus, five extracts (aerial and root extracts of non-parasited and parasited H. lasianthum and C. hypocistis) were evaluated. The tentative identification of both species comprises 39 phenolic compounds. Hydrolysable tannins and flavonoids were the main identified groups in C. hypocistis and H. lasianthum extracts, respectively. Regarding bioactivities, C. hypocistis exhibited excellent antioxidant results both in Oxidative Haemolysis (OxHLIA) and inhibition of Thiobarbituric Acid Reactive Substances Formation (TBARS). The tested extracts presented antimicrobial inhibition, anti-inflammatory activity, and effective cytotoxicity against tumour cells. C. hypocistis exhibited the lowest cytotoxicity on a non-tumour cell line. Principal Component Analysis (PCA) was a suitable approach to analyse differences among samples, explaining up to 67% of data variability and suggesting no similarities between parasite and host phenolic composition and bioactivities. Therefore, this comparative study emphasises the significance of both species as a source of biologically active compounds.
PB  - Amsterdam: Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter
VL  - 176
DO  - 10.1016/j.indcrop.2021.114343
SP  - 114343
ER  - 

@article{
author = "Silva, Ana Rita and Ayuso, Manuel and Pereira, Carla and Dias, Maria Inês and Kostić, Marina and Calhelha, Ricardo C. and Soković, Marina and García, Pablo A. and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2022",
abstract = "Cytinus hypocistis (L.) L. is a comestible holoparasite with great potential for cosmeceutical application. Although its high tannin content has been associated with its bioactive and inhibitory enzyme properties, this is the first report establishing a relationship between parasite and host (Halimium lasianthum (Lam.) Greuter) phenolic profile and bioactive properties. Thus, five extracts (aerial and root extracts of non-parasited and parasited H. lasianthum and C. hypocistis) were evaluated. The tentative identification of both species comprises 39 phenolic compounds. Hydrolysable tannins and flavonoids were the main identified groups in C. hypocistis and H. lasianthum extracts, respectively. Regarding bioactivities, C. hypocistis exhibited excellent antioxidant results both in Oxidative Haemolysis (OxHLIA) and inhibition of Thiobarbituric Acid Reactive Substances Formation (TBARS). The tested extracts presented antimicrobial inhibition, anti-inflammatory activity, and effective cytotoxicity against tumour cells. C. hypocistis exhibited the lowest cytotoxicity on a non-tumour cell line. Principal Component Analysis (PCA) was a suitable approach to analyse differences among samples, explaining up to 67% of data variability and suggesting no similarities between parasite and host phenolic composition and bioactivities. Therefore, this comparative study emphasises the significance of both species as a source of biologically active compounds.",
publisher = "Amsterdam: Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter",
volume = "176",
doi = "10.1016/j.indcrop.2021.114343",
pages = "114343"
}

Silva, A. R., Ayuso, M., Pereira, C., Dias, M. I., Kostić, M., Calhelha, R. C., Soković, M., García, P. A., Ferreira, I. C.F.R.,& Barros, L.. (2022). Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter. in Industrial Crops and Products
Amsterdam: Elsevier B.V.., 176, 114343.
https://doi.org/10.1016/j.indcrop.2021.114343

Silva AR, Ayuso M, Pereira C, Dias MI, Kostić M, Calhelha RC, Soković M, García PA, Ferreira IC, Barros L. Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter. in Industrial Crops and Products. 2022;176:114343.
doi:10.1016/j.indcrop.2021.114343 .

Silva, Ana Rita, Ayuso, Manuel, Pereira, Carla, Dias, Maria Inês, Kostić, Marina, Calhelha, Ricardo C., Soković, Marina, García, Pablo A., Ferreira, Isabel C.F.R., Barros, Lillian, "Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter" in Industrial Crops and Products, 176 (2022):114343,
https://doi.org/10.1016/j.indcrop.2021.114343 . .

TY  - JOUR
AU  - Alonso-Esteban, José Ignacio
AU  - Pinela, José
AU  - Ćirić, Ana
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
AU  - Torija-Isasa, Esperanza
AU  - Sánchez-Mata, María de Cortes
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814621027606
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4688
AB  - This study aimed to determine a complete chemical composition of eight different varieties of whole hemp seeds and eight samples of commercial dehulled hemp seeds. We also evaluated the phenolic profiles and antioxidant, cytotoxic, and antimicrobial properties of hydromethanolic seed extracts. Whole hemp seeds contain much more fibre than dehulled hemp seeds, which contain more fat and protein. Sucrose and raffinose were the most abundant soluble sugars, and citric and oxalic acids were the most abundant organic acids. In the hydromethanolic hemp seed extracts, we detected the phenolic acids ferulic acid-hexoside and syringic acid. Whole hemp seed extracts exhibited better antioxidant activity than dehulled hemp seed extracts, especially in the TBARS assay. Cytotoxic activity against NCI-H460 cells was also observed. The dehulled hemp seed extracts displayed antibacterial activity, especially against Bacillus cereus, Listeria monocytogenes, and Enterococcus faecalis, and antifungal activity to a lesser extent.
T2  - Food Chemistry
T1  - Chemical composition and biological activities of whole and dehulled hemp (Cannabis sativa L.) seeds
VL  - 374
DO  - 10.1016/j.foodchem.2021.131754
SP  - 131754
ER  - 

@article{
author = "Alonso-Esteban, José Ignacio and Pinela, José and Ćirić, Ana and Calhelha, Ricardo C. and Soković, Marina and Ferreira, Isabel C.F.R. and Barros, Lillian and Torija-Isasa, Esperanza and Sánchez-Mata, María de Cortes",
year = "2022",
abstract = "This study aimed to determine a complete chemical composition of eight different varieties of whole hemp seeds and eight samples of commercial dehulled hemp seeds. We also evaluated the phenolic profiles and antioxidant, cytotoxic, and antimicrobial properties of hydromethanolic seed extracts. Whole hemp seeds contain much more fibre than dehulled hemp seeds, which contain more fat and protein. Sucrose and raffinose were the most abundant soluble sugars, and citric and oxalic acids were the most abundant organic acids. In the hydromethanolic hemp seed extracts, we detected the phenolic acids ferulic acid-hexoside and syringic acid. Whole hemp seed extracts exhibited better antioxidant activity than dehulled hemp seed extracts, especially in the TBARS assay. Cytotoxic activity against NCI-H460 cells was also observed. The dehulled hemp seed extracts displayed antibacterial activity, especially against Bacillus cereus, Listeria monocytogenes, and Enterococcus faecalis, and antifungal activity to a lesser extent.",
journal = "Food Chemistry",
title = "Chemical composition and biological activities of whole and dehulled hemp (Cannabis sativa L.) seeds",
volume = "374",
doi = "10.1016/j.foodchem.2021.131754",
pages = "131754"
}

Alonso-Esteban, J. I., Pinela, J., Ćirić, A., Calhelha, R. C., Soković, M., Ferreira, I. C.F.R., Barros, L., Torija-Isasa, E.,& Sánchez-Mata, M. d. C.. (2022). Chemical composition and biological activities of whole and dehulled hemp (Cannabis sativa L.) seeds. in Food Chemistry, 374, 131754.
https://doi.org/10.1016/j.foodchem.2021.131754

Alonso-Esteban JI, Pinela J, Ćirić A, Calhelha RC, Soković M, Ferreira IC, Barros L, Torija-Isasa E, Sánchez-Mata MDC. Chemical composition and biological activities of whole and dehulled hemp (Cannabis sativa L.) seeds. in Food Chemistry. 2022;374:131754.
doi:10.1016/j.foodchem.2021.131754 .

Alonso-Esteban, José Ignacio, Pinela, José, Ćirić, Ana, Calhelha, Ricardo C., Soković, Marina, Ferreira, Isabel C.F.R., Barros, Lillian, Torija-Isasa, Esperanza, Sánchez-Mata, María de Cortes, "Chemical composition and biological activities of whole and dehulled hemp (Cannabis sativa L.) seeds" in Food Chemistry, 374 (2022):131754,
https://doi.org/10.1016/j.foodchem.2021.131754 . .

TY  - JOUR
AU  - Garcia-Manieri, Jéssica A.A
AU  - Correa, Vanesa Gesser
AU  - Corrêa, Rúbia Carvalho Gomes
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
AU  - Bracht, Adelar
AU  - Peralta, Rosane M.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1878818122002572
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5341
AB  - Leaves and barks of Luehea divaricata (Malvaceae) are used in folk medicine for the treatment of infectious diseases, tumors, rheumatism and diabetes. This work presents in vivo experiments (rats and mice) supported by in vitro and chemical analyses in order to address the above mentioned folk medicinal uses. The phenolic constituents of both aqueous and hydroalcoholic extracts are mainly catechin derivatives (135.4 and 215.9 mg/g, respectively). Both extracts displayed strong antioxidant activity. They also showed antiproliferative activities against tumor cells, nitric oxide synthesis inhibition (indicative of anti-inflammatory activity) and antimicrobial activity against a variety of infectious agents. Paracetamol-induced liver injury in rats, which has a high inflammatory component, was substantially attenuated in animals treated with the hydro-alcoholic extract (200 mg kg−1 day−1). This was deduced from aspartate aminotransferase and alanine aminotransferase measurements in plasma as well as from the hepatic activities of catalase and superoxide dismutase. Both extracts inhibited the pancreatic α-amylase with IC50 values of 11.50 ± 0.05 and 18.70 ± 3.87 μg/mL for the hydro-alcoholic and aqueous extract, respectively. Both extracts inhibited starch digestion in mice at the doses of 200 and 400 mg/kg. In general terms the results confirm several of the vox populi notions about the therapeutic potential of L. divaricata, the antidiabetic action, combined with the hepatoprotective effect, being the most relevant observations. Not less important is the economical perspective for the rural producers which may eventually increase their revenue by selling increasingly valued raw materials derived from L. divaricata based on its therapeutic properties.
PB  - Amsterdam: Elsevier
T2  - Biocatalysis and Agricultural Biotechnology
T1  - Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems
VL  - 45
DO  - 10.1016/j.bcab.2022.102530
SP  - 102530
ER  - 

@article{
author = "Garcia-Manieri, Jéssica A.A and Correa, Vanesa Gesser and Corrêa, Rúbia Carvalho Gomes and Dias, Maria Inês and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R. and Bracht, Adelar and Peralta, Rosane M.",
year = "2022",
abstract = "Leaves and barks of Luehea divaricata (Malvaceae) are used in folk medicine for the treatment of infectious diseases, tumors, rheumatism and diabetes. This work presents in vivo experiments (rats and mice) supported by in vitro and chemical analyses in order to address the above mentioned folk medicinal uses. The phenolic constituents of both aqueous and hydroalcoholic extracts are mainly catechin derivatives (135.4 and 215.9 mg/g, respectively). Both extracts displayed strong antioxidant activity. They also showed antiproliferative activities against tumor cells, nitric oxide synthesis inhibition (indicative of anti-inflammatory activity) and antimicrobial activity against a variety of infectious agents. Paracetamol-induced liver injury in rats, which has a high inflammatory component, was substantially attenuated in animals treated with the hydro-alcoholic extract (200 mg kg−1 day−1). This was deduced from aspartate aminotransferase and alanine aminotransferase measurements in plasma as well as from the hepatic activities of catalase and superoxide dismutase. Both extracts inhibited the pancreatic α-amylase with IC50 values of 11.50 ± 0.05 and 18.70 ± 3.87 μg/mL for the hydro-alcoholic and aqueous extract, respectively. Both extracts inhibited starch digestion in mice at the doses of 200 and 400 mg/kg. In general terms the results confirm several of the vox populi notions about the therapeutic potential of L. divaricata, the antidiabetic action, combined with the hepatoprotective effect, being the most relevant observations. Not less important is the economical perspective for the rural producers which may eventually increase their revenue by selling increasingly valued raw materials derived from L. divaricata based on its therapeutic properties.",
publisher = "Amsterdam: Elsevier",
journal = "Biocatalysis and Agricultural Biotechnology",
title = "Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems",
volume = "45",
doi = "10.1016/j.bcab.2022.102530",
pages = "102530"
}

Garcia-Manieri, J. A.A, Correa, V. G., Corrêa, R. C. G., Dias, M. I., Calhelha, R. C., Ivanov, M., Soković, M., Barros, L., Ferreira, I. C.F.R., Bracht, A.,& Peralta, R. M.. (2022). Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems. in Biocatalysis and Agricultural Biotechnology
Amsterdam: Elsevier., 45, 102530.
https://doi.org/10.1016/j.bcab.2022.102530

Garcia-Manieri JA, Correa VG, Corrêa RCG, Dias MI, Calhelha RC, Ivanov M, Soković M, Barros L, Ferreira IC, Bracht A, Peralta RM. Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems. in Biocatalysis and Agricultural Biotechnology. 2022;45:102530.
doi:10.1016/j.bcab.2022.102530 .

Garcia-Manieri, Jéssica A.A, Correa, Vanesa Gesser, Corrêa, Rúbia Carvalho Gomes, Dias, Maria Inês, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., Bracht, Adelar, Peralta, Rosane M., "Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems" in Biocatalysis and Agricultural Biotechnology, 45 (2022):102530,
https://doi.org/10.1016/j.bcab.2022.102530 . .

TY  - CONF
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Ćirić, Ana
AU  - Rocha, Filomena
AU  - Barata, Ana Maria
AU  - Soković, Marina
AU  - Carvalho, Ana Maria
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4374
AB  - Table tomato (Solanum lycopersicum L.) is highly consumed worldwide and its annual production generates 
a considerable amount of plant by-products [1]. Although tomatoes are well-known for their composition 
in nutrients and bioactive compounds [2,3], little information is available in literature regarding tomato 
leaves, probably because these by-products have no commercial value. Therefore, considering the enormous
production of this biomass and the current trends of circularity and sustainable development [3], this study 
was carried out to evaluate the polyphenolic composition and bioactive properties of tomato leaves resulting 
from the regeneration of table tomato landraces conserved ex-situ in the Portuguese Genebank (BPGV), in 
Braga, Portugal. The tomato plant material resulting from pruning performed after the flowering season was
lyophilized and ground to a fine powder, used to prepare hydroethanolic extracts [4]. These extracts were 
characterized for their composition in polyphenols (by HPLC-DAD-ESI/MSn
) and used to evaluate the 
antioxidant (by DPPH•
scavenging capacity and reduction power), antihemolytic (using sheep erythrocytes 
and AAPH as a free radical generator), and antimicrobial (tested against food-borne bacterial and fungal 
strains by the serial microdilution and p-iodonitrotetrazolium violet colorimetric methods) activities [4].
The chromatographic analysis allowed identifying phenolic acids and flavonoids, with prevalence of 
quercetin-3-O-rutinoside (rutin). The leaf extracts showed antioxidant activity, with EC50 values lower than 
those previously reported for the respective tomato fruits, thus translating a higher activity [2]. The extracts 
were also effective in some extent in protecting the erythrocytes from the oxidative hemolysis caused the 
thermal decomposition of the free-radical initiator AAPH. Despite their low activity against the tested 
microfungi, some extracts had ability to inhibit and kill some bacteria (including Salmonella typhimurium, 
Listeria monocytogenes, Bacillus cereus, and Enterobacter cloacae) more effectively than the antibiotic 
ampicillin. It was concluded that table tomato crop by-products can be used to produce rutin-rich extracts 
with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4374
ER  - 

@conference{
author = "Añibarro-Ortega, Mikel and Pinela, José and Ćirić, Ana and Rocha, Filomena and Barata, Ana Maria and Soković, Marina and Carvalho, Ana Maria and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Table tomato (Solanum lycopersicum L.) is highly consumed worldwide and its annual production generates 
a considerable amount of plant by-products [1]. Although tomatoes are well-known for their composition 
in nutrients and bioactive compounds [2,3], little information is available in literature regarding tomato 
leaves, probably because these by-products have no commercial value. Therefore, considering the enormous
production of this biomass and the current trends of circularity and sustainable development [3], this study 
was carried out to evaluate the polyphenolic composition and bioactive properties of tomato leaves resulting 
from the regeneration of table tomato landraces conserved ex-situ in the Portuguese Genebank (BPGV), in 
Braga, Portugal. The tomato plant material resulting from pruning performed after the flowering season was
lyophilized and ground to a fine powder, used to prepare hydroethanolic extracts [4]. These extracts were 
characterized for their composition in polyphenols (by HPLC-DAD-ESI/MSn
) and used to evaluate the 
antioxidant (by DPPH•
scavenging capacity and reduction power), antihemolytic (using sheep erythrocytes 
and AAPH as a free radical generator), and antimicrobial (tested against food-borne bacterial and fungal 
strains by the serial microdilution and p-iodonitrotetrazolium violet colorimetric methods) activities [4].
The chromatographic analysis allowed identifying phenolic acids and flavonoids, with prevalence of 
quercetin-3-O-rutinoside (rutin). The leaf extracts showed antioxidant activity, with EC50 values lower than 
those previously reported for the respective tomato fruits, thus translating a higher activity [2]. The extracts 
were also effective in some extent in protecting the erythrocytes from the oxidative hemolysis caused the 
thermal decomposition of the free-radical initiator AAPH. Despite their low activity against the tested 
microfungi, some extracts had ability to inhibit and kill some bacteria (including Salmonella typhimurium, 
Listeria monocytogenes, Bacillus cereus, and Enterobacter cloacae) more effectively than the antibiotic 
ampicillin. It was concluded that table tomato crop by-products can be used to produce rutin-rich extracts 
with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4374"
}

Añibarro-Ortega, M., Pinela, J., Ćirić, A., Rocha, F., Barata, A. M., Soković, M., Carvalho, A. M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_4374

Añibarro-Ortega M, Pinela J, Ćirić A, Rocha F, Barata AM, Soković M, Carvalho AM, Ferreira ICFR, Barros L. Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_4374 .

Añibarro-Ortega, Mikel, Pinela, José, Ćirić, Ana, Rocha, Filomena, Barata, Ana Maria, Soković, Marina, Carvalho, Ana Maria, Ferreira, Isabel C. F. R., Barros, Lillian, "Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):73,
https://hdl.handle.net/21.15107/rcub_ibiss_4374 .

TY  - JOUR
AU  - Lima, Juliana França
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Steinmacher, Nádia Cristiane
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2073-4395/11/9/1873
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4490
AB  - Consumers have given preferences to food products that, in addition to the nutritional properties, also present bioactive characteristics with beneficial health effects. The use of Nonconventional Food Plants (NCFP) has been an asset for the food industry, not only due to its abundance but, also, because it does not compete with other vegetable matrices used for human consumption for its nutritional properties, chemical and bioactive potentiality. The present work aimed to study the granulometry and water absorption index of three seeds of unconventional food plants: niger, millet and birdseed, followed by their nutritional value, content in free sugars, fatty acids, organic acids, tocopherols and phenolic compounds by chromatographic methods and, also, the evaluation of the antioxidant, hepatotoxic and antimicrobial potential in their hydroethanolic extracts. Bakery products were developed with a partial replacement of wheat flour, using the centroid simplex method to understand the effect of applying NCFP flours in the final physical–chemical characteristics. The high granulometry associated with the high water absorption index indicates that the use of NCFP flours would have to be complemented with other flours for baking. The chemical composition of niger seed stood out the most; however, all seeds presented relatively low IC50 and MIC values for the inhibition of lipid peroxidation and antimicrobial activity, respectively. Finally, breads made with a mixture of millet and birdseed flour showed the greatest similarity to the control bread. Considering their composition in bioactive compounds, the use of these seeds is highly advisable in the context of a fortified diet, being sources of compounds of high nutritional value and with beneficial health effects for the final consumer.
PB  - Basel: MDPI
T2  - Agronomy
T1  - Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry
IS  - 9
VL  - 11
DO  - 10.3390/agronomy11091873
SP  - 1873
ER  - 

@article{
author = "Lima, Juliana França and Dias, Maria Inês and Pereira, Carla and Ivanov, Marija and Soković, Marina and Steinmacher, Nádia Cristiane and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Consumers have given preferences to food products that, in addition to the nutritional properties, also present bioactive characteristics with beneficial health effects. The use of Nonconventional Food Plants (NCFP) has been an asset for the food industry, not only due to its abundance but, also, because it does not compete with other vegetable matrices used for human consumption for its nutritional properties, chemical and bioactive potentiality. The present work aimed to study the granulometry and water absorption index of three seeds of unconventional food plants: niger, millet and birdseed, followed by their nutritional value, content in free sugars, fatty acids, organic acids, tocopherols and phenolic compounds by chromatographic methods and, also, the evaluation of the antioxidant, hepatotoxic and antimicrobial potential in their hydroethanolic extracts. Bakery products were developed with a partial replacement of wheat flour, using the centroid simplex method to understand the effect of applying NCFP flours in the final physical–chemical characteristics. The high granulometry associated with the high water absorption index indicates that the use of NCFP flours would have to be complemented with other flours for baking. The chemical composition of niger seed stood out the most; however, all seeds presented relatively low IC50 and MIC values for the inhibition of lipid peroxidation and antimicrobial activity, respectively. Finally, breads made with a mixture of millet and birdseed flour showed the greatest similarity to the control bread. Considering their composition in bioactive compounds, the use of these seeds is highly advisable in the context of a fortified diet, being sources of compounds of high nutritional value and with beneficial health effects for the final consumer.",
publisher = "Basel: MDPI",
journal = "Agronomy",
title = "Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry",
number = "9",
volume = "11",
doi = "10.3390/agronomy11091873",
pages = "1873"
}

Lima, J. F., Dias, M. I., Pereira, C., Ivanov, M., Soković, M., Steinmacher, N. C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry. in Agronomy
Basel: MDPI., 11(9), 1873.
https://doi.org/10.3390/agronomy11091873

Lima JF, Dias MI, Pereira C, Ivanov M, Soković M, Steinmacher NC, Ferreira ICFR, Barros L. Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry. in Agronomy. 2021;11(9):1873.
doi:10.3390/agronomy11091873 .

Lima, Juliana França, Dias, Maria Inês, Pereira, Carla, Ivanov, Marija, Soković, Marina, Steinmacher, Nádia Cristiane, Ferreira, Isabel C. F. R., Barros, Lillian, "Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry" in Agronomy, 11, no. 9 (2021):1873,
https://doi.org/10.3390/agronomy11091873 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Kannan, Abhilash
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C.F.R.
AU  - Sanglard, Dominique
AU  - Soković, Marina
PY  - 2021
UR  - https://doi.org/10.3390/ijms22020483
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4230
AB  - Candida albicans represents one of the most common fungal pathogens. Due to its increasing incidence and the poor efficacy of available antifungals, finding novel antifungal molecules is of great importance. Camphor and eucalyptol are bioactive terpenoid plant constituents and their antifungal properties have been explored previously. In this study, we examined their ability to inhibit the growth of different Candida species in suspension and biofilm, to block hyphal transition along with their impact on genes encoding for efflux pumps (CDR1 and CDR2), ergosterol biosynthesis (ERG11), and cytotoxicity to primary liver cells. Camphor showed excellent antifungal activity with a minimal inhibitory concentration of 0.125–0.35 mg/mL while eucalyptol was active in the range of 2–23 mg/mL. The results showed camphor’s potential to reduce fungal virulence traits, that is, biofilm establishment and hyphae formation. On the other hand, camphor and eucalyptol treatments upregulated CDR1; CDR2 was positively regulated after eucalyptol application while camphor downregulated it. Neither had an impact on ERG11 expression. The beneficial antifungal activities of camphor were achieved with an amount that was non-toxic to porcine liver cells, making it a promising antifungal compound for future development. The antifungal concentration of eucalyptol caused cytotoxic effects and increased expression of efflux pump genes, which suggests that it is an unsuitable antifungal candidate.
PB  - MDPI AG
T2  - International Journal of Molecular Sciences
T1  - Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity
IS  - 2
VL  - 22
DO  - 10.3390/ijms22020483
SP  - 1
EP  - 14
ER  - 

@article{
author = "Ivanov, Marija and Kannan, Abhilash and Stojković, Dejan and Glamočlija, Jasmina and Calhelha, Ricardo C. and Ferreira, Isabel C.F.R. and Sanglard, Dominique and Soković, Marina",
year = "2021",
abstract = "Candida albicans represents one of the most common fungal pathogens. Due to its increasing incidence and the poor efficacy of available antifungals, finding novel antifungal molecules is of great importance. Camphor and eucalyptol are bioactive terpenoid plant constituents and their antifungal properties have been explored previously. In this study, we examined their ability to inhibit the growth of different Candida species in suspension and biofilm, to block hyphal transition along with their impact on genes encoding for efflux pumps (CDR1 and CDR2), ergosterol biosynthesis (ERG11), and cytotoxicity to primary liver cells. Camphor showed excellent antifungal activity with a minimal inhibitory concentration of 0.125–0.35 mg/mL while eucalyptol was active in the range of 2–23 mg/mL. The results showed camphor’s potential to reduce fungal virulence traits, that is, biofilm establishment and hyphae formation. On the other hand, camphor and eucalyptol treatments upregulated CDR1; CDR2 was positively regulated after eucalyptol application while camphor downregulated it. Neither had an impact on ERG11 expression. The beneficial antifungal activities of camphor were achieved with an amount that was non-toxic to porcine liver cells, making it a promising antifungal compound for future development. The antifungal concentration of eucalyptol caused cytotoxic effects and increased expression of efflux pump genes, which suggests that it is an unsuitable antifungal candidate.",
publisher = "MDPI AG",
journal = "International Journal of Molecular Sciences",
title = "Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity",
number = "2",
volume = "22",
doi = "10.3390/ijms22020483",
pages = "1-14"
}

Ivanov, M., Kannan, A., Stojković, D., Glamočlija, J., Calhelha, R. C., Ferreira, I. C.F.R., Sanglard, D.,& Soković, M.. (2021). Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity. in International Journal of Molecular Sciences
MDPI AG., 22(2), 1-14.
https://doi.org/10.3390/ijms22020483

Ivanov M, Kannan A, Stojković D, Glamočlija J, Calhelha RC, Ferreira IC, Sanglard D, Soković M. Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity. in International Journal of Molecular Sciences. 2021;22(2):1-14.
doi:10.3390/ijms22020483 .

Ivanov, Marija, Kannan, Abhilash, Stojković, Dejan, Glamočlija, Jasmina, Calhelha, Ricardo C., Ferreira, Isabel C.F.R., Sanglard, Dominique, Soković, Marina, "Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity" in International Journal of Molecular Sciences, 22, no. 2 (2021):1-14,
https://doi.org/10.3390/ijms22020483 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32781352
UR  - https://radar.ibiss.bg.ac.rs/123456789/3846
AB  - Cardoon (Cynara cardunculus L.) bracts were collected at different maturation stages to investigate seasonal changes in the phenolic compounds profile and in vitro bioactivities. Among the 12 phenolic compounds tentatively identified, 3,5-O-dicaffeoylquinic acid (21.83 mg/g extract) and apigenin-7-O-glucuronide (10.6 mg/g extract) were the most abundant. Immature bracts (C1: principal growth stage (PGS) 5) had the highest phenolic compounds content, and anti-inflammatory (IC50 = 72 µg/mL) and cytotoxic (GI50 of 30-79 µg/mL) activities. Moreover, extract C1 inhibited efficiently the formation of thiobarbituric acid reactive substances (TBARS; IC50 = 26.8 µg/mL), while extract C8 (PGS 8/9) was more effective against oxidative haemolysis (IC50 38 and 75 µg/mL). The highest antibacterial and antifungal activities were attributed to samples C1 and C6 (PGS 7/8) and samples C2 (PGS 5/6) and C4 (PGS 6/7), respectively. Overall, the obtained results suggest the seasonal changes of polyphenolic composition and bioactivity of cardoon bracts of variable maturity.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.
VL  - 336
DO  - 10.1016/j.foodchem.2020.127744
SP  - 127744
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Dias, Maria Inês and Pinela, José and Kostić, Marina and Soković, Marina and Santos-Buelga, Celestino and Ferreira, Isabel C F R and Barros, Lillian",
year = "2021",
abstract = "Cardoon (Cynara cardunculus L.) bracts were collected at different maturation stages to investigate seasonal changes in the phenolic compounds profile and in vitro bioactivities. Among the 12 phenolic compounds tentatively identified, 3,5-O-dicaffeoylquinic acid (21.83 mg/g extract) and apigenin-7-O-glucuronide (10.6 mg/g extract) were the most abundant. Immature bracts (C1: principal growth stage (PGS) 5) had the highest phenolic compounds content, and anti-inflammatory (IC50 = 72 µg/mL) and cytotoxic (GI50 of 30-79 µg/mL) activities. Moreover, extract C1 inhibited efficiently the formation of thiobarbituric acid reactive substances (TBARS; IC50 = 26.8 µg/mL), while extract C8 (PGS 8/9) was more effective against oxidative haemolysis (IC50 38 and 75 µg/mL). The highest antibacterial and antifungal activities were attributed to samples C1 and C6 (PGS 7/8) and samples C2 (PGS 5/6) and C4 (PGS 6/7), respectively. Overall, the obtained results suggest the seasonal changes of polyphenolic composition and bioactivity of cardoon bracts of variable maturity.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.",
volume = "336",
doi = "10.1016/j.foodchem.2020.127744",
pages = "127744"
}

Mandim, F., Petropoulos, S. A., Dias, M. I., Pinela, J., Kostić, M., Soković, M., Santos-Buelga, C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.. in Food Chemistry
Elsevier Ltd., 336, 127744.
https://doi.org/10.1016/j.foodchem.2020.127744

Mandim F, Petropoulos SA, Dias MI, Pinela J, Kostić M, Soković M, Santos-Buelga C, Ferreira ICFR, Barros L. Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.. in Food Chemistry. 2021;336:127744.
doi:10.1016/j.foodchem.2020.127744 .

Mandim, Filipa, Petropoulos, Spyridon A., Dias, Maria Inês, Pinela, José, Kostić, Marina, Soković, Marina, Santos-Buelga, Celestino, Ferreira, Isabel C F R, Barros, Lillian, "Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts." in Food Chemistry, 336 (2021):127744,
https://doi.org/10.1016/j.foodchem.2020.127744 . .

TY  - JOUR
AU  - Ribeiro, Ana S.
AU  - Costa, Sofia M.
AU  - Ferreira, Diana P.
AU  - Calhelha, Ricardo C.
AU  - Barros, Lillian
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ferreira, Isabel C.F.R.
AU  - Fangueiro, Raul
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4095
AB  - The combination of biodegradable fibers at nanoscale with plant-based extracts is attracting increasing attention to produce wound dressing systems. In this work, nanofibers based on chitosan (CS), poly(ethylene oxide) (PEO), cellulose nanocrystals (CNC) and acacia plant-based extract were developed by electrospinning. Firstly, the polymeric formulations and electrospinning parameters were optimized, resulting in nanofibers with average diameters of 80 nm. CNC were successfully introduced into the optimized CS/PEO blend and the membranes were characterized by FESEM, ATR-FTIR, TGA, XRD, WVTR and WCA. The CNC incorporation improved the nanofibers' physical integrity, morphology, diameters, water vapor transmission rate and thermal properties. After acacia introduction into the best CS/PEO/CNC system, the antibacterial effect was relatively maintained while the antifungal activity was enhanced for some fungi, demonstrating its great effect against a wide range of microorganisms, which is crucial to prevent or treat infections. All the developed systems exhibited absence of cytotoxicity in non-tumor cells, suggesting their biocompatibility. Finally, a continuous release of the acacia extract was observed for 24 h, showing its prolonged action, which contributes to the healing process while reduces the frequency of dressing's replacement. Overall, the developed nanofibers are very promising to act as localized drug delivery systems for wound care applications.
PB  - Elsevier B.V.
T2  - Reactive and Functional Polymers
T1  - Chitosan/nanocellulose electrospun fibers with enhanced antibacterial and antifungal activity for wound dressing applications
VL  - 159
DO  - 10.1016/j.reactfunctpolym.2020.104808
SP  - 104808
ER  - 

@article{
author = "Ribeiro, Ana S. and Costa, Sofia M. and Ferreira, Diana P. and Calhelha, Ricardo C. and Barros, Lillian and Stojković, Dejan and Soković, Marina and Ferreira, Isabel C.F.R. and Fangueiro, Raul",
year = "2021",
abstract = "The combination of biodegradable fibers at nanoscale with plant-based extracts is attracting increasing attention to produce wound dressing systems. In this work, nanofibers based on chitosan (CS), poly(ethylene oxide) (PEO), cellulose nanocrystals (CNC) and acacia plant-based extract were developed by electrospinning. Firstly, the polymeric formulations and electrospinning parameters were optimized, resulting in nanofibers with average diameters of 80 nm. CNC were successfully introduced into the optimized CS/PEO blend and the membranes were characterized by FESEM, ATR-FTIR, TGA, XRD, WVTR and WCA. The CNC incorporation improved the nanofibers' physical integrity, morphology, diameters, water vapor transmission rate and thermal properties. After acacia introduction into the best CS/PEO/CNC system, the antibacterial effect was relatively maintained while the antifungal activity was enhanced for some fungi, demonstrating its great effect against a wide range of microorganisms, which is crucial to prevent or treat infections. All the developed systems exhibited absence of cytotoxicity in non-tumor cells, suggesting their biocompatibility. Finally, a continuous release of the acacia extract was observed for 24 h, showing its prolonged action, which contributes to the healing process while reduces the frequency of dressing's replacement. Overall, the developed nanofibers are very promising to act as localized drug delivery systems for wound care applications.",
publisher = "Elsevier B.V.",
journal = "Reactive and Functional Polymers",
title = "Chitosan/nanocellulose electrospun fibers with enhanced antibacterial and antifungal activity for wound dressing applications",
volume = "159",
doi = "10.1016/j.reactfunctpolym.2020.104808",
pages = "104808"
}

Ribeiro, A. S., Costa, S. M., Ferreira, D. P., Calhelha, R. C., Barros, L., Stojković, D., Soković, M., Ferreira, I. C.F.R.,& Fangueiro, R.. (2021). Chitosan/nanocellulose electrospun fibers with enhanced antibacterial and antifungal activity for wound dressing applications. in Reactive and Functional Polymers
Elsevier B.V.., 159, 104808.
https://doi.org/10.1016/j.reactfunctpolym.2020.104808

Ribeiro AS, Costa SM, Ferreira DP, Calhelha RC, Barros L, Stojković D, Soković M, Ferreira IC, Fangueiro R. Chitosan/nanocellulose electrospun fibers with enhanced antibacterial and antifungal activity for wound dressing applications. in Reactive and Functional Polymers. 2021;159:104808.
doi:10.1016/j.reactfunctpolym.2020.104808 .

Ribeiro, Ana S., Costa, Sofia M., Ferreira, Diana P., Calhelha, Ricardo C., Barros, Lillian, Stojković, Dejan, Soković, Marina, Ferreira, Isabel C.F.R., Fangueiro, Raul, "Chitosan/nanocellulose electrospun fibers with enhanced antibacterial and antifungal activity for wound dressing applications" in Reactive and Functional Polymers, 159 (2021):104808,
https://doi.org/10.1016/j.reactfunctpolym.2020.104808 . .

TY  - JOUR
AU  - García-Pérez, Pascual
AU  - Ayuso, Manuel
AU  - Lozano-Milo, Eva
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Ivanov, Marija
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
AU  - Gallego, Pedro P.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4207
AB  - Bryophyllum constitutes a subgenus within the genus Kalanchoe that contains several plant species used in traditional medicine worldwide for the treatment of several diseases. However, little is known about the phytoconstituents of Bryophyllum spp. and previous reports have pointed at their low in Planta concentrations of bioactive compounds. In this work, we take advantage of plant in vitro culture for the study of the phenolic compounds found in the aerial parts of Bryophyllum spp. and their associated bioactivities. Our results show that the induction of nutritional stress leads to an improved accumulation of phenolic compounds, mainly flavonols and anthocyanins, represented by myricetin and malvidin glycosides, respectively. This effect is mainly found for B. × houghtonii, whose hydroethanolic extracts promoted the highest antioxidant, cytotoxic and anti-inflammatory activities. In the case of cytotoxic activity, Bryophyllum extracts showed an enhanced activity against the MCF-7 human breast carcinoma cell line. Meanwhile, extracts from B. daigremontianum promoted a wide range of effectiveness against different bacterial and fungal strains. This study is committed to shed light about the phytochemical potential associated to this unexplored subgenus, with the aim of considering Bryophyllum spp. as a valuable source of bioactive compounds for their exploitation in food, cosmetic and pharmaceutical industries.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants
VL  - 162
DO  - 10.1016/j.indcrop.2021.113241
SP  - 113241
ER  - 

@article{
author = "García-Pérez, Pascual and Ayuso, Manuel and Lozano-Milo, Eva and Pereira, Carla and Dias, Maria Inês and Ivanov, Marija and Calhelha, Ricardo C. and Soković, Marina and Ferreira, Isabel C.F.R. and Barros, Lillian and Gallego, Pedro P.",
year = "2021",
abstract = "Bryophyllum constitutes a subgenus within the genus Kalanchoe that contains several plant species used in traditional medicine worldwide for the treatment of several diseases. However, little is known about the phytoconstituents of Bryophyllum spp. and previous reports have pointed at their low in Planta concentrations of bioactive compounds. In this work, we take advantage of plant in vitro culture for the study of the phenolic compounds found in the aerial parts of Bryophyllum spp. and their associated bioactivities. Our results show that the induction of nutritional stress leads to an improved accumulation of phenolic compounds, mainly flavonols and anthocyanins, represented by myricetin and malvidin glycosides, respectively. This effect is mainly found for B. × houghtonii, whose hydroethanolic extracts promoted the highest antioxidant, cytotoxic and anti-inflammatory activities. In the case of cytotoxic activity, Bryophyllum extracts showed an enhanced activity against the MCF-7 human breast carcinoma cell line. Meanwhile, extracts from B. daigremontianum promoted a wide range of effectiveness against different bacterial and fungal strains. This study is committed to shed light about the phytochemical potential associated to this unexplored subgenus, with the aim of considering Bryophyllum spp. as a valuable source of bioactive compounds for their exploitation in food, cosmetic and pharmaceutical industries.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants",
volume = "162",
doi = "10.1016/j.indcrop.2021.113241",
pages = "113241"
}

García-Pérez, P., Ayuso, M., Lozano-Milo, E., Pereira, C., Dias, M. I., Ivanov, M., Calhelha, R. C., Soković, M., Ferreira, I. C.F.R., Barros, L.,& Gallego, P. P.. (2021). Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants. in Industrial Crops and Products
Elsevier B.V.., 162, 113241.
https://doi.org/10.1016/j.indcrop.2021.113241

García-Pérez P, Ayuso M, Lozano-Milo E, Pereira C, Dias MI, Ivanov M, Calhelha RC, Soković M, Ferreira IC, Barros L, Gallego PP. Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants. in Industrial Crops and Products. 2021;162:113241.
doi:10.1016/j.indcrop.2021.113241 .

García-Pérez, Pascual, Ayuso, Manuel, Lozano-Milo, Eva, Pereira, Carla, Dias, Maria Inês, Ivanov, Marija, Calhelha, Ricardo C., Soković, Marina, Ferreira, Isabel C.F.R., Barros, Lillian, Gallego, Pedro P., "Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants" in Industrial Crops and Products, 162 (2021):113241,
https://doi.org/10.1016/j.indcrop.2021.113241 . .

TY  - JOUR
AU  - Bertéli, Míria Benetati Delgado
AU  - Barros, Lillian
AU  - Reis, Filipa S.
AU  - Ferreira, Isabel C. F. R.
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Valle, Juliana Silveira
AU  - Linde, Giani Andrea
AU  - Ruiz, Suelen Pereira
AU  - Colauto, Nelson Barros
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4301
AB  - Lentinus crinitus (L.) Fr. (Basidiomycota: Polyporales) is a wild mushroom with several biotechnological
applications; however, there are few studies on its chemical composition and antimicrobial activity.
Therefore, this study aims to evaluate the chemical composition, cytotoxicity, and antimicrobial activity of
L. crinitus basidiocarp. For that, its nutritional value (AOAC procedures) and its composition in some
hydrophilic and lipophilic compounds (chromatographic techniques) were assessed. Moreover, the
potential hepatotoxic effects were evaluated using a primary cell culture obtained from porcine liver, and
its growth inhibitory capacity was also evaluated against four human tumour cell lines (spectrophotometric
assays). The antimicrobial activity was evaluated by microdilution against eight bacteria and fungi.
The basidiocarp has a high content of carbohydrates and, therefore, a relatively high energetic value. It is
also rich in soluble sugars, β-tocopherol, phenolic acids, mainly p-hydroxybenzoic acid, and organic
acids, mainly malic acid. L. crinitus did not show cytotoxicity in non-tumour cells, but it did not inhibit the
growth of human tumour cell lines either. The basidiocarp has a wide antimicrobial activity, inhibiting the
growth of different species of bacteria and fungi. It showed minimum bactericidal and fungicidal concentration values similar to or lower than those verified by commercial antibiotics or food additives used as preservatives. The antimicrobial activity was more evident against Listeria monocytogenes, Salmonella enterica, and Penicillium ochrochloron, followed by Aspergillus ochraceus and Trichoderma viride, when compared to the controls. The results obtained in this study showed that L. crinitus basidiocarp has great potential to be used by the industry without toxicity risks.
PB  - The Royal Society of Chemistry
T2  - Food & Function
T1  - Antimicrobial activity, chemical composition and cytotoxicity of Lentinus crinitus basidiocarp
DO  - 10.1039/d1fo00656h
ER  - 

@article{
author = "Bertéli, Míria Benetati Delgado and Barros, Lillian and Reis, Filipa S. and Ferreira, Isabel C. F. R. and Glamočlija, Jasmina and Soković, Marina and Valle, Juliana Silveira and Linde, Giani Andrea and Ruiz, Suelen Pereira and Colauto, Nelson Barros",
year = "2021",
abstract = "Lentinus crinitus (L.) Fr. (Basidiomycota: Polyporales) is a wild mushroom with several biotechnological
applications; however, there are few studies on its chemical composition and antimicrobial activity.
Therefore, this study aims to evaluate the chemical composition, cytotoxicity, and antimicrobial activity of
L. crinitus basidiocarp. For that, its nutritional value (AOAC procedures) and its composition in some
hydrophilic and lipophilic compounds (chromatographic techniques) were assessed. Moreover, the
potential hepatotoxic effects were evaluated using a primary cell culture obtained from porcine liver, and
its growth inhibitory capacity was also evaluated against four human tumour cell lines (spectrophotometric
assays). The antimicrobial activity was evaluated by microdilution against eight bacteria and fungi.
The basidiocarp has a high content of carbohydrates and, therefore, a relatively high energetic value. It is
also rich in soluble sugars, β-tocopherol, phenolic acids, mainly p-hydroxybenzoic acid, and organic
acids, mainly malic acid. L. crinitus did not show cytotoxicity in non-tumour cells, but it did not inhibit the
growth of human tumour cell lines either. The basidiocarp has a wide antimicrobial activity, inhibiting the
growth of different species of bacteria and fungi. It showed minimum bactericidal and fungicidal concentration values similar to or lower than those verified by commercial antibiotics or food additives used as preservatives. The antimicrobial activity was more evident against Listeria monocytogenes, Salmonella enterica, and Penicillium ochrochloron, followed by Aspergillus ochraceus and Trichoderma viride, when compared to the controls. The results obtained in this study showed that L. crinitus basidiocarp has great potential to be used by the industry without toxicity risks.",
publisher = "The Royal Society of Chemistry",
journal = "Food & Function",
title = "Antimicrobial activity, chemical composition and cytotoxicity of Lentinus crinitus basidiocarp",
doi = "10.1039/d1fo00656h"
}

Bertéli, M. B. D., Barros, L., Reis, F. S., Ferreira, I. C. F. R., Glamočlija, J., Soković, M., Valle, J. S., Linde, G. A., Ruiz, S. P.,& Colauto, N. B.. (2021). Antimicrobial activity, chemical composition and cytotoxicity of Lentinus crinitus basidiocarp. in Food & Function
The Royal Society of Chemistry..
https://doi.org/10.1039/d1fo00656h

Bertéli MBD, Barros L, Reis FS, Ferreira ICFR, Glamočlija J, Soković M, Valle JS, Linde GA, Ruiz SP, Colauto NB. Antimicrobial activity, chemical composition and cytotoxicity of Lentinus crinitus basidiocarp. in Food & Function. 2021;.
doi:10.1039/d1fo00656h .

Bertéli, Míria Benetati Delgado, Barros, Lillian, Reis, Filipa S., Ferreira, Isabel C. F. R., Glamočlija, Jasmina, Soković, Marina, Valle, Juliana Silveira, Linde, Giani Andrea, Ruiz, Suelen Pereira, Colauto, Nelson Barros, "Antimicrobial activity, chemical composition and cytotoxicity of Lentinus crinitus basidiocarp" in Food & Function (2021),
https://doi.org/10.1039/d1fo00656h . .

TY  - JOUR
AU  - Carpena, Marija
AU  - Caleja, Cristina
AU  - Pereira, Eliana
AU  - Pereira, Carla
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Soria-Lopez, Anton
AU  - Fraga-Corral, Maria
AU  - Simal-Gandara, Jesus
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Prieto, Miguel A.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4359
AB  - The present work aimed to determine the nutritional composition (ash, protein, fat, car bohydrate content and energy value), phenolic compounds, pigments and organic acids content
of three typical red algae from the Northwest of Spain: Chondrus crispus, Mastocarpus stellatus, and
Gigartina pistillata; as well as their antioxidant and antimicrobial activities. Furthermore, the present
work compared two extraction techniques: conventional heat assisted extraction (HAE) and high
pressure assisted extraction (HPAE) to maximize the yield and the concentration of target compounds.
Different independent variables were considered for the response study. Time (t) and percentage
of ethanol of the solvent (S) were chosen for both techniques and temperature (T) and pressure (P)
were used for HAE and HPAE, respectively. The experiments were designed following a response
surface methodology (RSM) approach. The obtained results showed a similar nutritional composition
between algae samples: low-fat content and high content of proteins, carbohydrates and energy. All
tested algae showed good antioxidant and antimicrobial properties. Finally, HEA demonstrated to
be the most efficient extraction technique. This study confirms the potential of red algae to be part
of the human diet as a source of non-animal protein, due to its nutritional content, phenolic profile,
pigments concentration and bioactive properties, which proves that HAE is the optimum technique
for the extraction maximization.
PB  - Basel: MDPI
T2  - Chemosensors
T1  - Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction
IS  - 6
VL  - 9
DO  - 10.3390/chemosensors9060132
SP  - 132
ER  - 

@article{
author = "Carpena, Marija and Caleja, Cristina and Pereira, Eliana and Pereira, Carla and Ćirić, Ana and Soković, Marina and Soria-Lopez, Anton and Fraga-Corral, Maria and Simal-Gandara, Jesus and Ferreira, Isabel C. F. R. and Barros, Lillian and Prieto, Miguel A.",
year = "2021",
abstract = "The present work aimed to determine the nutritional composition (ash, protein, fat, car bohydrate content and energy value), phenolic compounds, pigments and organic acids content
of three typical red algae from the Northwest of Spain: Chondrus crispus, Mastocarpus stellatus, and
Gigartina pistillata; as well as their antioxidant and antimicrobial activities. Furthermore, the present
work compared two extraction techniques: conventional heat assisted extraction (HAE) and high
pressure assisted extraction (HPAE) to maximize the yield and the concentration of target compounds.
Different independent variables were considered for the response study. Time (t) and percentage
of ethanol of the solvent (S) were chosen for both techniques and temperature (T) and pressure (P)
were used for HAE and HPAE, respectively. The experiments were designed following a response
surface methodology (RSM) approach. The obtained results showed a similar nutritional composition
between algae samples: low-fat content and high content of proteins, carbohydrates and energy. All
tested algae showed good antioxidant and antimicrobial properties. Finally, HEA demonstrated to
be the most efficient extraction technique. This study confirms the potential of red algae to be part
of the human diet as a source of non-animal protein, due to its nutritional content, phenolic profile,
pigments concentration and bioactive properties, which proves that HAE is the optimum technique
for the extraction maximization.",
publisher = "Basel: MDPI",
journal = "Chemosensors",
title = "Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction",
number = "6",
volume = "9",
doi = "10.3390/chemosensors9060132",
pages = "132"
}

Carpena, M., Caleja, C., Pereira, E., Pereira, C., Ćirić, A., Soković, M., Soria-Lopez, A., Fraga-Corral, M., Simal-Gandara, J., Ferreira, I. C. F. R., Barros, L.,& Prieto, M. A.. (2021). Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction. in Chemosensors
Basel: MDPI., 9(6), 132.
https://doi.org/10.3390/chemosensors9060132

Carpena M, Caleja C, Pereira E, Pereira C, Ćirić A, Soković M, Soria-Lopez A, Fraga-Corral M, Simal-Gandara J, Ferreira ICFR, Barros L, Prieto MA. Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction. in Chemosensors. 2021;9(6):132.
doi:10.3390/chemosensors9060132 .

Carpena, Marija, Caleja, Cristina, Pereira, Eliana, Pereira, Carla, Ćirić, Ana, Soković, Marina, Soria-Lopez, Anton, Fraga-Corral, Maria, Simal-Gandara, Jesus, Ferreira, Isabel C. F. R., Barros, Lillian, Prieto, Miguel A., "Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction" in Chemosensors, 9, no. 6 (2021):132,
https://doi.org/10.3390/chemosensors9060132 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Liberal, Ângela
AU  - Pinela, José
AU  - Finimundy, Tiane C.
AU  - Bancessi, Aducabe
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Catarino, Luís
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2212429221004259
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4462
AB  - Moringa oleifera Lam. is a nutraceutical edible plant used to fight malnutrition in developing countries. This investigation describes the nutritional, chemical and bioactive assets of wild and commercially acquired M. oleifera leaves from Guinea-Bissau. Both samples presented significant differences in the contents of almost all the analysed parameters. Carbohydrates and proteins were the major constituents of the leaves, which also showed an interesting profile of tocopherols, organic acids, unsaturated fatty acids, and phenolic compounds. The infused extract of the commercial leaf sample was more effective than other preparations against lipid peroxidation and oxidative haemolysis. In turn, higher antibacterial activity was achieved with hydroethanolic and infused extracts of the wild sample, which extracts also displayed superior antifungal activity. Overall, the commercially acquired M. oleifera leaves stand out with better quality profiles than the wild collected, which aroused interest in studying the processing methods used locally to process this functional food.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - Compositional features and biological activities of wild and commercial Moringa oleifera leaves from Guinea-Bissau
VL  - 43
DO  - 10.1016/j.fbio.2021.101300
SP  - 101300
ER  - 

@article{
author = "Fernandes, Ângela and Liberal, Ângela and Pinela, José and Finimundy, Tiane C. and Bancessi, Aducabe and Ćirić, Ana and Soković, Marina and Catarino, Luís and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2021",
abstract = "Moringa oleifera Lam. is a nutraceutical edible plant used to fight malnutrition in developing countries. This investigation describes the nutritional, chemical and bioactive assets of wild and commercially acquired M. oleifera leaves from Guinea-Bissau. Both samples presented significant differences in the contents of almost all the analysed parameters. Carbohydrates and proteins were the major constituents of the leaves, which also showed an interesting profile of tocopherols, organic acids, unsaturated fatty acids, and phenolic compounds. The infused extract of the commercial leaf sample was more effective than other preparations against lipid peroxidation and oxidative haemolysis. In turn, higher antibacterial activity was achieved with hydroethanolic and infused extracts of the wild sample, which extracts also displayed superior antifungal activity. Overall, the commercially acquired M. oleifera leaves stand out with better quality profiles than the wild collected, which aroused interest in studying the processing methods used locally to process this functional food.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "Compositional features and biological activities of wild and commercial Moringa oleifera leaves from Guinea-Bissau",
volume = "43",
doi = "10.1016/j.fbio.2021.101300",
pages = "101300"
}

Fernandes, Â., Liberal, Â., Pinela, J., Finimundy, T. C., Bancessi, A., Ćirić, A., Soković, M., Catarino, L., Ferreira, I. C.F.R.,& Barros, L.. (2021). Compositional features and biological activities of wild and commercial Moringa oleifera leaves from Guinea-Bissau. in Food Bioscience
Elsevier Ltd., 43, 101300.
https://doi.org/10.1016/j.fbio.2021.101300

Fernandes Â, Liberal Â, Pinela J, Finimundy TC, Bancessi A, Ćirić A, Soković M, Catarino L, Ferreira IC, Barros L. Compositional features and biological activities of wild and commercial Moringa oleifera leaves from Guinea-Bissau. in Food Bioscience. 2021;43:101300.
doi:10.1016/j.fbio.2021.101300 .

Fernandes, Ângela, Liberal, Ângela, Pinela, José, Finimundy, Tiane C., Bancessi, Aducabe, Ćirić, Ana, Soković, Marina, Catarino, Luís, Ferreira, Isabel C.F.R., Barros, Lillian, "Compositional features and biological activities of wild and commercial Moringa oleifera leaves from Guinea-Bissau" in Food Bioscience, 43 (2021):101300,
https://doi.org/10.1016/j.fbio.2021.101300 . .

TY  - JOUR
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Ćirić, Ana
AU  - Lopes, Elsa
AU  - Molina, Adriana K.
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Ferreira, Olga
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2079-7737/10/10/951
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4488
AB  - Aloesin is an aromatic chromone with increasing applications in the cosmetic and health food industries. To optimize its extraction from the Aloe vera leaf rind, the independent variables time (10–210 min), temperature (25–95 °C) and organic solvent composition (0–100%, w/w) were combined in a central composite design coupled with response surface methodology. The solvents consisted of binary mixtures of water with ethanol, propylene glycol, or glycerol. The aloesin levels quantified in each extract were used as response for optimization. The theoretical models were fitted to the experimental data, statistically validated, and used to obtain the optimal extraction conditions. Then, a dose–response analysis of the solid/liquid ratio (S/L) was performed under the optimal conditions determined for each alcohol–water system and revealed that a linear improvement in extraction efficiency can be achieved by increasing the S/L ratio by up to 40 g/L. This analysis also allowed to experimentally validate the predictive models. Furthermore, the aloesin-rich extracts revealed antioxidant activity through thiobarbituric acid reactive substances (TBARS) formation inhibition, antimicrobial effects against bacterial and fungal strains, and no toxicity for PLP2 cells. Overall, this study provided optimal extraction conditions for the recovery of aloesin from Aloe vera rind through an eco-friendly extraction process and highlighted its bioactive potential.
PB  - Basel: MDPI
T2  - Biology
T1  - Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment
IS  - 10
VL  - 10
DO  - 10.3390/biology10100951
SP  - 951
ER  - 

@article{
author = "Añibarro-Ortega, Mikel and Pinela, José and Ćirić, Ana and Lopes, Elsa and Molina, Adriana K. and Calhelha, Ricardo C. and Soković, Marina and Ferreira, Olga and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Aloesin is an aromatic chromone with increasing applications in the cosmetic and health food industries. To optimize its extraction from the Aloe vera leaf rind, the independent variables time (10–210 min), temperature (25–95 °C) and organic solvent composition (0–100%, w/w) were combined in a central composite design coupled with response surface methodology. The solvents consisted of binary mixtures of water with ethanol, propylene glycol, or glycerol. The aloesin levels quantified in each extract were used as response for optimization. The theoretical models were fitted to the experimental data, statistically validated, and used to obtain the optimal extraction conditions. Then, a dose–response analysis of the solid/liquid ratio (S/L) was performed under the optimal conditions determined for each alcohol–water system and revealed that a linear improvement in extraction efficiency can be achieved by increasing the S/L ratio by up to 40 g/L. This analysis also allowed to experimentally validate the predictive models. Furthermore, the aloesin-rich extracts revealed antioxidant activity through thiobarbituric acid reactive substances (TBARS) formation inhibition, antimicrobial effects against bacterial and fungal strains, and no toxicity for PLP2 cells. Overall, this study provided optimal extraction conditions for the recovery of aloesin from Aloe vera rind through an eco-friendly extraction process and highlighted its bioactive potential.",
publisher = "Basel: MDPI",
journal = "Biology",
title = "Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment",
number = "10",
volume = "10",
doi = "10.3390/biology10100951",
pages = "951"
}

Añibarro-Ortega, M., Pinela, J., Ćirić, A., Lopes, E., Molina, A. K., Calhelha, R. C., Soković, M., Ferreira, O., Ferreira, I. C. F. R.,& Barros, L.. (2021). Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment. in Biology
Basel: MDPI., 10(10), 951.
https://doi.org/10.3390/biology10100951

Añibarro-Ortega M, Pinela J, Ćirić A, Lopes E, Molina AK, Calhelha RC, Soković M, Ferreira O, Ferreira ICFR, Barros L. Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment. in Biology. 2021;10(10):951.
doi:10.3390/biology10100951 .

Añibarro-Ortega, Mikel, Pinela, José, Ćirić, Ana, Lopes, Elsa, Molina, Adriana K., Calhelha, Ricardo C., Soković, Marina, Ferreira, Olga, Ferreira, Isabel C. F. R., Barros, Lillian, "Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment" in Biology, 10, no. 10 (2021):951,
https://doi.org/10.3390/biology10100951 . .

TY  - CONF
AU  - Molina, Adriana K.
AU  - Vega, Erika N.
AU  - Pereira, Karla
AU  - Dias, Maria Inês
AU  - Heleno, Sandrina A.
AU  - Rodriges, Paula
AU  - Calhelha, Ricardo C.
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Barreira, Joao C.M.
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4930
AB  - Lonicera caerulea L., Morus nigra L., and Rubus fruticosus L. fruits are widely known for their nutritional
and bioactive properties. Their richness in anthocyanins, which are the main responsible compounds for
the reported beneficial properties, justify their exploitation not only as functional foods but also as sources
of natural colorants, in alternative to some artificial compounds with reported adverse effects to human
health [1,2]. In this context, the fruits were characterized in terms of anthocyanin and non-anthocyanin
compounds, by HPLC-DAD/ESI-MS, and two solid colouring formulations were prepared through the
spray-drying technique with maltodextrin and mixtures of Arabic gum and maltodextrin in different
proportions, according to the characteristics of each fruit juice and the efficiency of the process. The
stability of the prepared colorants was assessed over three months of storage at room and refrigerated
temperature. For that purpose, the microbial load, the cytotoxicity, and the bioactive properties
(antioxidant and antimicrobial) were evaluated, along with their anthocyanin concentration and colouring
capacity.
Different phenolic compounds were detected in the three fruits, among which, some anthocyanins as
cyanidin-3- O -glucoside and cyanidin- O -hexose, as the most abundant ones. All the formulations revealed
great colouring, antioxidant, and antimicrobial properties, with a slight variation of anthocyanin
concentration along the three months of storage at room and refrigerated temperature, which validate their
application for colouring purposes. None of these formulations revealed cytotoxic properties, being, then,
considered safe for food application. Through the present study, it was possible to obtain stable
anthocyanin based colorants with potential application in several industrial fields.
PB  - Bragança: Instituto Politécnico de Bragança
C3  - 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online
T1  - Bioactive food colourants from Lonicera caerulea L., Morus nigra L., and Rubus fruticosus fruits
SP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4930
ER  - 

@conference{
author = "Molina, Adriana K. and Vega, Erika N. and Pereira, Karla and Dias, Maria Inês and Heleno, Sandrina A. and Rodriges, Paula and Calhelha, Ricardo C. and Kostić, Marina and Soković, Marina and Barreira, Joao C.M. and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2021",
abstract = "Lonicera caerulea L., Morus nigra L., and Rubus fruticosus L. fruits are widely known for their nutritional
and bioactive properties. Their richness in anthocyanins, which are the main responsible compounds for
the reported beneficial properties, justify their exploitation not only as functional foods but also as sources
of natural colorants, in alternative to some artificial compounds with reported adverse effects to human
health [1,2]. In this context, the fruits were characterized in terms of anthocyanin and non-anthocyanin
compounds, by HPLC-DAD/ESI-MS, and two solid colouring formulations were prepared through the
spray-drying technique with maltodextrin and mixtures of Arabic gum and maltodextrin in different
proportions, according to the characteristics of each fruit juice and the efficiency of the process. The
stability of the prepared colorants was assessed over three months of storage at room and refrigerated
temperature. For that purpose, the microbial load, the cytotoxicity, and the bioactive properties
(antioxidant and antimicrobial) were evaluated, along with their anthocyanin concentration and colouring
capacity.
Different phenolic compounds were detected in the three fruits, among which, some anthocyanins as
cyanidin-3- O -glucoside and cyanidin- O -hexose, as the most abundant ones. All the formulations revealed
great colouring, antioxidant, and antimicrobial properties, with a slight variation of anthocyanin
concentration along the three months of storage at room and refrigerated temperature, which validate their
application for colouring purposes. None of these formulations revealed cytotoxic properties, being, then,
considered safe for food application. Through the present study, it was possible to obtain stable
anthocyanin based colorants with potential application in several industrial fields.",
publisher = "Bragança: Instituto Politécnico de Bragança",
journal = "1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online",
title = "Bioactive food colourants from Lonicera caerulea L., Morus nigra L., and Rubus fruticosus fruits",
pages = "171",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4930"
}

Molina, A. K., Vega, E. N., Pereira, K., Dias, M. I., Heleno, S. A., Rodriges, P., Calhelha, R. C., Kostić, M., Soković, M., Barreira, J. C.M., Ferreira, I. C.F.R.,& Barros, L.. (2021). Bioactive food colourants from Lonicera caerulea L., Morus nigra L., and Rubus fruticosus fruits. in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online
Bragança: Instituto Politécnico de Bragança., 171.
https://hdl.handle.net/21.15107/rcub_ibiss_4930

Molina AK, Vega EN, Pereira K, Dias MI, Heleno SA, Rodriges P, Calhelha RC, Kostić M, Soković M, Barreira JC, Ferreira IC, Barros L. Bioactive food colourants from Lonicera caerulea L., Morus nigra L., and Rubus fruticosus fruits. in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online. 2021;:171.
https://hdl.handle.net/21.15107/rcub_ibiss_4930 .

Molina, Adriana K., Vega, Erika N., Pereira, Karla, Dias, Maria Inês, Heleno, Sandrina A., Rodriges, Paula, Calhelha, Ricardo C., Kostić, Marina, Soković, Marina, Barreira, Joao C.M., Ferreira, Isabel C.F.R., Barros, Lillian, "Bioactive food colourants from Lonicera caerulea L., Morus nigra L., and Rubus fruticosus fruits" in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online (2021):171,
https://hdl.handle.net/21.15107/rcub_ibiss_4930 .

TY  - JOUR
AU  - Sampaio, Shirley L.
AU  - Lonchamp, Julien
AU  - Dias, Maria Inês
AU  - Liddle, Catriona
AU  - Petropoulos, Spyridon A.
AU  - Glamočlija, Jasmina
AU  - Alexopoulos, Alexios
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814620323888
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4041
AB  - Aqueous extracts from seven coloured potato varieties (three red-fleshed, three-purple fleshed, and one marble-fleshed) were studied for their anthocyanin content, in vitro biological activities, colouring properties and their potential application in the food industry. Acylated glycosides or pelargonidin and petunidin aglycones were identified as the main anthocyanin forms in the red and purple varieties, respectively. The total anthocyanin content among varieties ranged from 478.3 to 886.2 mg/100 g extract. All the extracts presented in vitro antioxidant, antibacterial and antifungal activities, whereas no toxic effects were detected. Finally, two selected extracts were tested as colourants in a soft drink formulation and presented suitable sensory profiles as well as high colour stability during a 30-day shelf-life when compared with the commercial colourant E163. Therefore, the tested extracts could be used as natural food colourants and considered for substituting the existing synthetic colouring agents.
PB  - Elsevier BV
T2  - Food Chemistry
T1  - Anthocyanin-rich extracts from purple and red potatoes as natural colourants: Bioactive properties, application in a soft drink formulation and sensory analysis.
VL  - 342
DO  - 10.1016/j.foodchem.2020.128526
SP  - 128526
ER  - 

@article{
author = "Sampaio, Shirley L. and Lonchamp, Julien and Dias, Maria Inês and Liddle, Catriona and Petropoulos, Spyridon A. and Glamočlija, Jasmina and Alexopoulos, Alexios and Santos-Buelga, Celestino and Ferreira, Isabel C F R and Barros, Lillian",
year = "2021",
abstract = "Aqueous extracts from seven coloured potato varieties (three red-fleshed, three-purple fleshed, and one marble-fleshed) were studied for their anthocyanin content, in vitro biological activities, colouring properties and their potential application in the food industry. Acylated glycosides or pelargonidin and petunidin aglycones were identified as the main anthocyanin forms in the red and purple varieties, respectively. The total anthocyanin content among varieties ranged from 478.3 to 886.2 mg/100 g extract. All the extracts presented in vitro antioxidant, antibacterial and antifungal activities, whereas no toxic effects were detected. Finally, two selected extracts were tested as colourants in a soft drink formulation and presented suitable sensory profiles as well as high colour stability during a 30-day shelf-life when compared with the commercial colourant E163. Therefore, the tested extracts could be used as natural food colourants and considered for substituting the existing synthetic colouring agents.",
publisher = "Elsevier BV",
journal = "Food Chemistry",
title = "Anthocyanin-rich extracts from purple and red potatoes as natural colourants: Bioactive properties, application in a soft drink formulation and sensory analysis.",
volume = "342",
doi = "10.1016/j.foodchem.2020.128526",
pages = "128526"
}

Sampaio, S. L., Lonchamp, J., Dias, M. I., Liddle, C., Petropoulos, S. A., Glamočlija, J., Alexopoulos, A., Santos-Buelga, C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Anthocyanin-rich extracts from purple and red potatoes as natural colourants: Bioactive properties, application in a soft drink formulation and sensory analysis.. in Food Chemistry
Elsevier BV., 342, 128526.
https://doi.org/10.1016/j.foodchem.2020.128526

Sampaio SL, Lonchamp J, Dias MI, Liddle C, Petropoulos SA, Glamočlija J, Alexopoulos A, Santos-Buelga C, Ferreira ICFR, Barros L. Anthocyanin-rich extracts from purple and red potatoes as natural colourants: Bioactive properties, application in a soft drink formulation and sensory analysis.. in Food Chemistry. 2021;342:128526.
doi:10.1016/j.foodchem.2020.128526 .

Sampaio, Shirley L., Lonchamp, Julien, Dias, Maria Inês, Liddle, Catriona, Petropoulos, Spyridon A., Glamočlija, Jasmina, Alexopoulos, Alexios, Santos-Buelga, Celestino, Ferreira, Isabel C F R, Barros, Lillian, "Anthocyanin-rich extracts from purple and red potatoes as natural colourants: Bioactive properties, application in a soft drink formulation and sensory analysis." in Food Chemistry, 342 (2021):128526,
https://doi.org/10.1016/j.foodchem.2020.128526 . .

TY  - CONF
AU  - Pires Júnior, Eleomar O.
AU  - Caleja, Cristina
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo
AU  - Garcia, Carolina C.
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4380
AB  - Edible flowers have been described as presenting several health benefits, associated with powerful
bioactive compounds present in their composition [1]. These matrices have also been explored and applied
in several fields, such as in cosmetic, pharmaceutical, but specially in gastronomy, due to the with
increasing demand of the common consumer for safer and healthier food products [2]. The plants from the
genus Impatiens are popularly known for the attractive colours of the petals, and preliminary studies have
demonstrated a high potential bioactivity of these plants [3]. Thus, the present investigation aimed to
characterize the nutritional value (ash, protein, fat, and carbohydrate content, and energy value, by AOAC
methodology) and the phenolic compounds profile (by High-Performance Liquid Chromatography
coupled with a diode array detector and mass spectrometry by electrospray ionization -
HPLC-DAD-ESI/MS) of Impatiens balsamina L. pink petals. Moreover, the antioxidant, antimicrobial,
cytotoxic, and anti-inflammatory evaluation (by the oxidative hemolysis inhibition assay - OxHLIA,
microdilution method with ATCC strains, the sulforhodamine B method in four human tumour cell lines,
and analysis in macrophage cells of rats (RAW 264.7), to inhibit the production of NO, respectively) in
the hydroethanolic extracts was also accessed. Finally, the enriched-coloured extract was applied as a
colorant in a cake filling called "bombocas", and its colorant capacity was compared with an artificial
additive (E163). In the pink petals, proteins stood out as the main macronutrient, and only fructose and
glucose were found in sugars profile. As for the phenolic composition, eighteen compounds were
tentatively identified, five non-anthocyanin compounds (caffeic and coumaric acids, and
eryodictiol-O-hexoside) and ten anthocyanin compounds (mainly acylated O-glycosylated malvidin,
pelargonidin, and peonidin derivatives). In addition, the hydroethanolic extracts demonstrated
anti-inflammatory and cytotoxicity for all cell lines studied, presenting also a remarkable antifungal
activity. Finally, the coloured extract applied in the formulations conferred a more natural colour to the
"bombocas, as also functional properties such as antioxidant activity. Further studies will be performed in
order to optimize the extractions conditions of colorant compounds, requiring also stabilization strategies
for further evaluation of the colorant extract efficacy.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - Chemical and bioactive characterization of Impatiens balsamina L. pink flowers and their application in a portuguese pastry product
SP  - 192
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4380
ER  - 

@conference{
author = "Pires Júnior, Eleomar O. and Caleja, Cristina and Pinela, José and Dias, Maria Inês and Calhelha, Ricardo and Garcia, Carolina C. and Ćirić, Ana and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Edible flowers have been described as presenting several health benefits, associated with powerful
bioactive compounds present in their composition [1]. These matrices have also been explored and applied
in several fields, such as in cosmetic, pharmaceutical, but specially in gastronomy, due to the with
increasing demand of the common consumer for safer and healthier food products [2]. The plants from the
genus Impatiens are popularly known for the attractive colours of the petals, and preliminary studies have
demonstrated a high potential bioactivity of these plants [3]. Thus, the present investigation aimed to
characterize the nutritional value (ash, protein, fat, and carbohydrate content, and energy value, by AOAC
methodology) and the phenolic compounds profile (by High-Performance Liquid Chromatography
coupled with a diode array detector and mass spectrometry by electrospray ionization -
HPLC-DAD-ESI/MS) of Impatiens balsamina L. pink petals. Moreover, the antioxidant, antimicrobial,
cytotoxic, and anti-inflammatory evaluation (by the oxidative hemolysis inhibition assay - OxHLIA,
microdilution method with ATCC strains, the sulforhodamine B method in four human tumour cell lines,
and analysis in macrophage cells of rats (RAW 264.7), to inhibit the production of NO, respectively) in
the hydroethanolic extracts was also accessed. Finally, the enriched-coloured extract was applied as a
colorant in a cake filling called "bombocas", and its colorant capacity was compared with an artificial
additive (E163). In the pink petals, proteins stood out as the main macronutrient, and only fructose and
glucose were found in sugars profile. As for the phenolic composition, eighteen compounds were
tentatively identified, five non-anthocyanin compounds (caffeic and coumaric acids, and
eryodictiol-O-hexoside) and ten anthocyanin compounds (mainly acylated O-glycosylated malvidin,
pelargonidin, and peonidin derivatives). In addition, the hydroethanolic extracts demonstrated
anti-inflammatory and cytotoxicity for all cell lines studied, presenting also a remarkable antifungal
activity. Finally, the coloured extract applied in the formulations conferred a more natural colour to the
"bombocas, as also functional properties such as antioxidant activity. Further studies will be performed in
order to optimize the extractions conditions of colorant compounds, requiring also stabilization strategies
for further evaluation of the colorant extract efficacy.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "Chemical and bioactive characterization of Impatiens balsamina L. pink flowers and their application in a portuguese pastry product",
pages = "192",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4380"
}

Pires Júnior, E. O., Caleja, C., Pinela, J., Dias, M. I., Calhelha, R., Garcia, C. C., Ćirić, A., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Chemical and bioactive characterization of Impatiens balsamina L. pink flowers and their application in a portuguese pastry product. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 192.
https://hdl.handle.net/21.15107/rcub_ibiss_4380

Pires Júnior EO, Caleja C, Pinela J, Dias MI, Calhelha R, Garcia CC, Ćirić A, Soković M, Ferreira ICFR, Barros L. Chemical and bioactive characterization of Impatiens balsamina L. pink flowers and their application in a portuguese pastry product. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:192.
https://hdl.handle.net/21.15107/rcub_ibiss_4380 .

Pires Júnior, Eleomar O., Caleja, Cristina, Pinela, José, Dias, Maria Inês, Calhelha, Ricardo, Garcia, Carolina C., Ćirić, Ana, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "Chemical and bioactive characterization of Impatiens balsamina L. pink flowers and their application in a portuguese pastry product" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):192,
https://hdl.handle.net/21.15107/rcub_ibiss_4380 .

TY  - JOUR
AU  - Liberal, Ângela
AU  - Ângela, Fernandes
AU  - Polyzos, Nikolaos
AU  - Petropoulos, Spyridon
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4056
AB  - Petroselinum  crispum  Mill.,  Fuss.,  is  a  culinary  vegetable  used  as  an  aromatic  herb  that  garnishes  and  flavours  a  great  variety  of  dishes.  In  the  present  study,  the  chemical  profiles  and  bioactivities  of  leaf  samples  from  25  cultivars  (three  types:  plain-  and  curly-leafed  and  turnip-rooted)  from  this  species  were  assessed.  Seven  phenolic  compounds  were  identified  in  all  the  varieties, including apigenin and kaempherol derivates. Apigenin-O-pentoside-O-hexoside was the major  compound  in  all  the  tested  parsley  types  (20,  22  and  13  mg/g  of  extract,  respectively)  and  responsible  for  its  excellent  antioxidant  activity,  also  investigated  in  this  study.  Antimicrobial  activities  were  also  explored,  and  the  results  revealed  a  good  bioactivity  against  specific  tested  pathogens, such as bacteria and fungi. In conclusion, the leaves of all the types of P. crispum are a good source of natural bioactive compounds that confer health benefits, and thus, they should be part of a balanced and diversified diet.
PB  - Basel: MDPI
T2  - Molecules (Basel, Switzerland)
T1  - Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars
IS  - 23
VL  - 25
DO  - 10.3390/molecules25235606
SP  - 5606
ER  - 

@article{
author = "Liberal, Ângela and Ângela, Fernandes and Polyzos, Nikolaos and Petropoulos, Spyridon and Dias, Maria Inês and Pinela, José and Petrović, Jovana and Soković, Marina and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2020",
abstract = "Petroselinum  crispum  Mill.,  Fuss.,  is  a  culinary  vegetable  used  as  an  aromatic  herb  that  garnishes  and  flavours  a  great  variety  of  dishes.  In  the  present  study,  the  chemical  profiles  and  bioactivities  of  leaf  samples  from  25  cultivars  (three  types:  plain-  and  curly-leafed  and  turnip-rooted)  from  this  species  were  assessed.  Seven  phenolic  compounds  were  identified  in  all  the  varieties, including apigenin and kaempherol derivates. Apigenin-O-pentoside-O-hexoside was the major  compound  in  all  the  tested  parsley  types  (20,  22  and  13  mg/g  of  extract,  respectively)  and  responsible  for  its  excellent  antioxidant  activity,  also  investigated  in  this  study.  Antimicrobial  activities  were  also  explored,  and  the  results  revealed  a  good  bioactivity  against  specific  tested  pathogens, such as bacteria and fungi. In conclusion, the leaves of all the types of P. crispum are a good source of natural bioactive compounds that confer health benefits, and thus, they should be part of a balanced and diversified diet.",
publisher = "Basel: MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars",
number = "23",
volume = "25",
doi = "10.3390/molecules25235606",
pages = "5606"
}

Liberal, Â., Ângela, F., Polyzos, N., Petropoulos, S., Dias, M. I., Pinela, J., Petrović, J., Soković, M., Ferreira, I. C.F.R.,& Barros, L.. (2020). Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars. in Molecules (Basel, Switzerland)
Basel: MDPI., 25(23), 5606.
https://doi.org/10.3390/molecules25235606

Liberal Â, Ângela F, Polyzos N, Petropoulos S, Dias MI, Pinela J, Petrović J, Soković M, Ferreira IC, Barros L. Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars. in Molecules (Basel, Switzerland). 2020;25(23):5606.
doi:10.3390/molecules25235606 .

Liberal, Ângela, Ângela, Fernandes, Polyzos, Nikolaos, Petropoulos, Spyridon, Dias, Maria Inês, Pinela, José, Petrović, Jovana, Soković, Marina, Ferreira, Isabel C.F.R., Barros, Lillian, "Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars" in Molecules (Basel, Switzerland), 25, no. 23 (2020):5606,
https://doi.org/10.3390/molecules25235606 . .

TY  - JOUR
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Ćirić, Ana
AU  - Martins, Valter
AU  - Rocha, Filomena
AU  - Soković, Marina
AU  - Barata, Ana Maria
AU  - Carvalho, Ana Maria
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0960308520305174
UR  - https://radar.ibiss.bg.ac.rs/123456789/3906
AB  - Little attention has been paid to the phenolic composition and bioactive properties of primary tomato plant by-products (Solanum lycopersicum L.) as compared to fruits. In this study, axillary green shoots resulting from pruning and aerial biomass at the end of the cultivation cycle were characterized for their composition in chlorophylls and phenolic compounds, as well as for antioxidant and antimicrobial activities. The HPLC-DAD-ESI/MSn analysis allowed identifying phenolic acids and flavonoids, with prevalence of quercetin-3-O-rutinoside (rutin). The extracts had in vitro antioxidant and antihemolytic activity, especially those produced from pruning materials. Despite their low activity against the tested microfungi, some extracts had ability to inhibit and kill some bacteria more effectively than the antibiotic ampicillin. It was interesting to conclude that table tomato crop remains, currently with no commercial value, can be used to produce extracts with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.
PB  - Elsevier BV
T2  - Food and Bioproducts Processing
T1  - Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities
VL  - 124
DO  - 10.1016/j.fbp.2020.09.006
SP  - 307
EP  - 319
ER  - 

@article{
author = "Añibarro-Ortega, Mikel and Pinela, José and Ćirić, Ana and Martins, Valter and Rocha, Filomena and Soković, Marina and Barata, Ana Maria and Carvalho, Ana Maria and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "Little attention has been paid to the phenolic composition and bioactive properties of primary tomato plant by-products (Solanum lycopersicum L.) as compared to fruits. In this study, axillary green shoots resulting from pruning and aerial biomass at the end of the cultivation cycle were characterized for their composition in chlorophylls and phenolic compounds, as well as for antioxidant and antimicrobial activities. The HPLC-DAD-ESI/MSn analysis allowed identifying phenolic acids and flavonoids, with prevalence of quercetin-3-O-rutinoside (rutin). The extracts had in vitro antioxidant and antihemolytic activity, especially those produced from pruning materials. Despite their low activity against the tested microfungi, some extracts had ability to inhibit and kill some bacteria more effectively than the antibiotic ampicillin. It was interesting to conclude that table tomato crop remains, currently with no commercial value, can be used to produce extracts with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.",
publisher = "Elsevier BV",
journal = "Food and Bioproducts Processing",
title = "Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities",
volume = "124",
doi = "10.1016/j.fbp.2020.09.006",
pages = "307-319"
}

Añibarro-Ortega, M., Pinela, J., Ćirić, A., Martins, V., Rocha, F., Soković, M., Barata, A. M., Carvalho, A. M., Barros, L.,& Ferreira, I. C.F.R.. (2020). Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities. in Food and Bioproducts Processing
Elsevier BV., 124, 307-319.
https://doi.org/10.1016/j.fbp.2020.09.006

Añibarro-Ortega M, Pinela J, Ćirić A, Martins V, Rocha F, Soković M, Barata AM, Carvalho AM, Barros L, Ferreira IC. Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities. in Food and Bioproducts Processing. 2020;124:307-319.
doi:10.1016/j.fbp.2020.09.006 .

Añibarro-Ortega, Mikel, Pinela, José, Ćirić, Ana, Martins, Valter, Rocha, Filomena, Soković, Marina, Barata, Ana Maria, Carvalho, Ana Maria, Barros, Lillian, Ferreira, Isabel C.F.R., "Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities" in Food and Bioproducts Processing, 124 (2020):307-319,
https://doi.org/10.1016/j.fbp.2020.09.006 . .

TY  - JOUR
AU  - Pereira, Eliana
AU  - Cadavez, Vasco
AU  - Barros, Lillian
AU  - Encina-Zelada, Christian
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Gonzales-Barron, Ursula
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3832
AB  - The ingestion of bioactive compounds has revealed health benefits, namely in the prevention and/or treatment of several diseases. This work aims to characterize the phenolic profile of three colour varieties of Chenopodium quinoa Willd. grains (black, red and white), and also evaluate their cytotoxic and antimicrobial activity. All varieties revealed the presence of phenolic compounds, namely, quercetin and kaempferol derivatives. In this study, quinoa grains did not reveal any anti-proliferative capacity in tumour cell lines, and, as expected, they were devoid of any toxicity. All of the analysed extracts possessed antibacterial and antifungal activities (inhibitory and bactericidal/fungicidal) against the microbial strains considered, exhibiting promising values of minimum bactericidal concentrations (mean MBC 0.153–0.916 mg/mL) and minimum fungicidal concentrations (mean MFC 0.211–0.884 mg/mL). Quinoa varieties represent a good source of bioactive compounds, interfering beneficially in the organism, specifically as antimicrobial agents. Thus, these extracts could be used in the development of bioactive ingredients.
PB  - Elsevier Ltd
T2  - Food Research International
T1  - Chenopodium quinoa Willd. (quinoa) grains: A good source of phenolic compounds
VL  - 137
DO  - 10.1016/j.foodres.2020.109574
SP  - 109574
ER  - 

@article{
author = "Pereira, Eliana and Cadavez, Vasco and Barros, Lillian and Encina-Zelada, Christian and Stojković, Dejan and Soković, Marina and Calhelha, Ricardo C. and Gonzales-Barron, Ursula and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "The ingestion of bioactive compounds has revealed health benefits, namely in the prevention and/or treatment of several diseases. This work aims to characterize the phenolic profile of three colour varieties of Chenopodium quinoa Willd. grains (black, red and white), and also evaluate their cytotoxic and antimicrobial activity. All varieties revealed the presence of phenolic compounds, namely, quercetin and kaempferol derivatives. In this study, quinoa grains did not reveal any anti-proliferative capacity in tumour cell lines, and, as expected, they were devoid of any toxicity. All of the analysed extracts possessed antibacterial and antifungal activities (inhibitory and bactericidal/fungicidal) against the microbial strains considered, exhibiting promising values of minimum bactericidal concentrations (mean MBC 0.153–0.916 mg/mL) and minimum fungicidal concentrations (mean MFC 0.211–0.884 mg/mL). Quinoa varieties represent a good source of bioactive compounds, interfering beneficially in the organism, specifically as antimicrobial agents. Thus, these extracts could be used in the development of bioactive ingredients.",
publisher = "Elsevier Ltd",
journal = "Food Research International",
title = "Chenopodium quinoa Willd. (quinoa) grains: A good source of phenolic compounds",
volume = "137",
doi = "10.1016/j.foodres.2020.109574",
pages = "109574"
}

Pereira, E., Cadavez, V., Barros, L., Encina-Zelada, C., Stojković, D., Soković, M., Calhelha, R. C., Gonzales-Barron, U.,& Ferreira, I. C.F.R.. (2020). Chenopodium quinoa Willd. (quinoa) grains: A good source of phenolic compounds. in Food Research International
Elsevier Ltd., 137, 109574.
https://doi.org/10.1016/j.foodres.2020.109574

Pereira E, Cadavez V, Barros L, Encina-Zelada C, Stojković D, Soković M, Calhelha RC, Gonzales-Barron U, Ferreira IC. Chenopodium quinoa Willd. (quinoa) grains: A good source of phenolic compounds. in Food Research International. 2020;137:109574.
doi:10.1016/j.foodres.2020.109574 .

Pereira, Eliana, Cadavez, Vasco, Barros, Lillian, Encina-Zelada, Christian, Stojković, Dejan, Soković, Marina, Calhelha, Ricardo C., Gonzales-Barron, Ursula, Ferreira, Isabel C.F.R., "Chenopodium quinoa Willd. (quinoa) grains: A good source of phenolic compounds" in Food Research International, 137 (2020):109574,
https://doi.org/10.1016/j.foodres.2020.109574 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/14/3175
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32664565
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3829
AB  - The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.
PB  - NLM (Medline)
T2  - Molecules (Basel, Switzerland)
T1  - The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.
IS  - 14
VL  - 25
DO  - 10.3390/molecules25143175
SP  - 3175
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C F R and Barros, Lillian",
year = "2020",
abstract = "The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.",
publisher = "NLM (Medline)",
journal = "Molecules (Basel, Switzerland)",
title = "The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.",
number = "14",
volume = "25",
doi = "10.3390/molecules25143175",
pages = "3175"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.. in Molecules (Basel, Switzerland)
NLM (Medline)., 25(14), 3175.
https://doi.org/10.3390/molecules25143175

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Ivanov M, Soković M, Ferreira ICFR, Barros L. The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.. in Molecules (Basel, Switzerland). 2020;25(14):3175.
doi:10.3390/molecules25143175 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Ferreira, Isabel C F R, Barros, Lillian, "The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark." in Molecules (Basel, Switzerland), 25, no. 14 (2020):3175,
https://doi.org/10.3390/molecules25143175 . .

TY  - JOUR
AU  - Finimundy, Tiane C.
AU  - Karkanis, Anestis
AU  - Fernandes, Ângela
AU  - Petropoulos, Spyridon A.
AU  - Calhelha, Ricardo
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Rosa, Eduardo
AU  - Barros, Lillian
AU  - Ferreira, Isabel C F R
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32470801
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3694
AB  - In this study, the chemical characterization and bioactive properties of S. minor cultivated under different fertilization rates (control, half rate and full rate) were evaluated. Twenty-two phenolic compounds were identified, including five phenolic acids, seven flavonoids and ten tannins. Hydrolysable tannins were prevalent, namely Sanguiin H-10, especially in leaves without fertilization (control). Roots of full-rate fertilizer (660 Kg/ha) presented the highest flavonoid content, mainly catechin and its isomers, whereas half-rate fertilizer (330 Kg/ha), presented the highest content of total phenolic compounds, due to the higher amount of ellagitannins (lambertianin C: 84 ± 1 mg/g of dry extract). Antimicrobial activities were also promising, especially against Salmonella typhimurium (MBC = 0.44 mg/mL). Moreover, root samples revealed activity against all tested cell lines regardless of fertilization rate, whereas leaves were effective only against HeLa cell line. In conclusion, S. minor could be a source of natural bioactive compounds, while fertilization could increase phenolic compounds content.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.
VL  - 327
DO  - 10.1016/j.foodchem.2020.127043
SP  - 127043
ER  - 

@article{
author = "Finimundy, Tiane C. and Karkanis, Anestis and Fernandes, Ângela and Petropoulos, Spyridon A. and Calhelha, Ricardo and Petrović, Jovana and Soković, Marina and Rosa, Eduardo and Barros, Lillian and Ferreira, Isabel C F R",
year = "2020",
abstract = "In this study, the chemical characterization and bioactive properties of S. minor cultivated under different fertilization rates (control, half rate and full rate) were evaluated. Twenty-two phenolic compounds were identified, including five phenolic acids, seven flavonoids and ten tannins. Hydrolysable tannins were prevalent, namely Sanguiin H-10, especially in leaves without fertilization (control). Roots of full-rate fertilizer (660 Kg/ha) presented the highest flavonoid content, mainly catechin and its isomers, whereas half-rate fertilizer (330 Kg/ha), presented the highest content of total phenolic compounds, due to the higher amount of ellagitannins (lambertianin C: 84 ± 1 mg/g of dry extract). Antimicrobial activities were also promising, especially against Salmonella typhimurium (MBC = 0.44 mg/mL). Moreover, root samples revealed activity against all tested cell lines regardless of fertilization rate, whereas leaves were effective only against HeLa cell line. In conclusion, S. minor could be a source of natural bioactive compounds, while fertilization could increase phenolic compounds content.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.",
volume = "327",
doi = "10.1016/j.foodchem.2020.127043",
pages = "127043"
}

Finimundy, T. C., Karkanis, A., Fernandes, Â., Petropoulos, S. A., Calhelha, R., Petrović, J., Soković, M., Rosa, E., Barros, L.,& Ferreira, I. C. F. R.. (2020). Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.. in Food Chemistry
Elsevier Ltd., 327, 127043.
https://doi.org/10.1016/j.foodchem.2020.127043

Finimundy TC, Karkanis A, Fernandes Â, Petropoulos SA, Calhelha R, Petrović J, Soković M, Rosa E, Barros L, Ferreira ICFR. Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.. in Food Chemistry. 2020;327:127043.
doi:10.1016/j.foodchem.2020.127043 .

Finimundy, Tiane C., Karkanis, Anestis, Fernandes, Ângela, Petropoulos, Spyridon A., Calhelha, Ricardo, Petrović, Jovana, Soković, Marina, Rosa, Eduardo, Barros, Lillian, Ferreira, Isabel C F R, "Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes." in Food Chemistry, 327 (2020):127043,
https://doi.org/10.1016/j.foodchem.2020.127043 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Giannoulis, Kyriakos D.
AU  - Dias, Maria Inês
AU  - Fernandes, Ângela
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0963996920303069
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3674
AB  - Cynara cardunculus L. (cardoon) has several health benefits mainly attributed to its abundance in polyphenols. In this study, cardoon heads (capitula) were harvested in Greece during the flowering stage, and the hydroethanolic extracts were assessed in terms of phenolic compounds composition and antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities. The phenolic profile was evaluated by HPLC-DAD-ESI/MS to better understand the seasonal changes in the individual compound levels and how these changes correlate with bioactive properties. The main phenolic compounds identified were caffeoylquinic and dicaffeoylquinic acid derivatives. Immature heads (Car A: principal growth stage (PGS) 5) had the highest phenolic content (34.3 mg/g) and cytotoxic (GI50 of 69–268 µg/mL) and anti-inflammatory (IC50 of 183 µg/mL) activities. Sample Car D (PGS 6/7) revealed the highest antioxidant (IC50 of 23–227 µg/mL) and antifungal (MIC of 0.26–0.51 mg/mL) potential. Regarding the antibacterial activity, Car E (PGS 7) revealed the best results (MIC of 0.59–1.18 mg/mL). This study suggests that the maturity stage of the plant influences the phenolic composition and bioactivity.
PB  - Elsevier Ltd
T2  - Food Research International
T1  - Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis
VL  - 134
DO  - 10.1016/j.foodres.2020.109281
SP  - 109281
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Giannoulis, Kyriakos D. and Dias, Maria Inês and Fernandes, Ângela and Pinela, José and Kostić, Marina and Soković, Marina and Barros, Lillian and Santos-Buelga, Celestino and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "Cynara cardunculus L. (cardoon) has several health benefits mainly attributed to its abundance in polyphenols. In this study, cardoon heads (capitula) were harvested in Greece during the flowering stage, and the hydroethanolic extracts were assessed in terms of phenolic compounds composition and antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities. The phenolic profile was evaluated by HPLC-DAD-ESI/MS to better understand the seasonal changes in the individual compound levels and how these changes correlate with bioactive properties. The main phenolic compounds identified were caffeoylquinic and dicaffeoylquinic acid derivatives. Immature heads (Car A: principal growth stage (PGS) 5) had the highest phenolic content (34.3 mg/g) and cytotoxic (GI50 of 69–268 µg/mL) and anti-inflammatory (IC50 of 183 µg/mL) activities. Sample Car D (PGS 6/7) revealed the highest antioxidant (IC50 of 23–227 µg/mL) and antifungal (MIC of 0.26–0.51 mg/mL) potential. Regarding the antibacterial activity, Car E (PGS 7) revealed the best results (MIC of 0.59–1.18 mg/mL). This study suggests that the maturity stage of the plant influences the phenolic composition and bioactivity.",
publisher = "Elsevier Ltd",
journal = "Food Research International",
title = "Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis",
volume = "134",
doi = "10.1016/j.foodres.2020.109281",
pages = "109281"
}

Mandim, F., Petropoulos, S. A., Giannoulis, K. D., Dias, M. I., Fernandes, Â., Pinela, J., Kostić, M., Soković, M., Barros, L., Santos-Buelga, C.,& Ferreira, I. C.F.R.. (2020). Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis. in Food Research International
Elsevier Ltd., 134, 109281.
https://doi.org/10.1016/j.foodres.2020.109281

Mandim F, Petropoulos SA, Giannoulis KD, Dias MI, Fernandes Â, Pinela J, Kostić M, Soković M, Barros L, Santos-Buelga C, Ferreira IC. Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis. in Food Research International. 2020;134:109281.
doi:10.1016/j.foodres.2020.109281 .

Mandim, Filipa, Petropoulos, Spyridon A., Giannoulis, Kyriakos D., Dias, Maria Inês, Fernandes, Ângela, Pinela, José, Kostić, Marina, Soković, Marina, Barros, Lillian, Santos-Buelga, Celestino, Ferreira, Isabel C.F.R., "Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis" in Food Research International, 134 (2020):109281,
https://doi.org/10.1016/j.foodres.2020.109281 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Barracosa, Paulo
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3659
AB  - Cardoon is a multi-purpose crop for several industries. In this study, cardoon seeds were separated according to the viability and characterized for their chemical composition and bioactivities. Viable seeds contained higher levels of α-tocopherol (6.7 mg/100 g), lipids (23.11 g/100 g, manly oleic and linoleic acids), and free sugars (5.4 g/100 g) than the unviable ones. The hydroethanolic extract of viable seeds presented a higher concentration of phenolic compounds, namely 5-O-caffeoylquinic (8.0 mg/g) and 3,5-O-dicaffeoylquinic (43.9 mg/g) acids, and greater in vitro antioxidant activity. Both extracts showed antibacterial properties, but the best results were achieved with unviable seeds. The extracts had similar antifungal activity but did not reveal anti-inflammatory capacity or cytotoxicity to the tested cell lines. Therefore, while viable seeds contained antioxidant phytochemicals and an energy and carbon source for germination, unavailable seeds stood out for their potential to be used in the development of bio-based antibacterial ingredients.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability: Chemical prospection and bioactivity of cardoon seeds
VL  - 323
DO  - 10.1016/j.foodchem.2020.126838
SP  - 126838
ER  - 

@article{
author = "Mandim, Filipa and Dias, Maria Inês and Pinela, José and Barracosa, Paulo and Ivanov, Marija and Stojković, Dejan and Soković, Marina and Santos-Buelga, Celestino and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "Cardoon is a multi-purpose crop for several industries. In this study, cardoon seeds were separated according to the viability and characterized for their chemical composition and bioactivities. Viable seeds contained higher levels of α-tocopherol (6.7 mg/100 g), lipids (23.11 g/100 g, manly oleic and linoleic acids), and free sugars (5.4 g/100 g) than the unviable ones. The hydroethanolic extract of viable seeds presented a higher concentration of phenolic compounds, namely 5-O-caffeoylquinic (8.0 mg/g) and 3,5-O-dicaffeoylquinic (43.9 mg/g) acids, and greater in vitro antioxidant activity. Both extracts showed antibacterial properties, but the best results were achieved with unviable seeds. The extracts had similar antifungal activity but did not reveal anti-inflammatory capacity or cytotoxicity to the tested cell lines. Therefore, while viable seeds contained antioxidant phytochemicals and an energy and carbon source for germination, unavailable seeds stood out for their potential to be used in the development of bio-based antibacterial ingredients.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability: Chemical prospection and bioactivity of cardoon seeds",
volume = "323",
doi = "10.1016/j.foodchem.2020.126838",
pages = "126838"
}

Mandim, F., Dias, M. I., Pinela, J., Barracosa, P., Ivanov, M., Stojković, D., Soković, M., Santos-Buelga, C., Barros, L.,& Ferreira, I. C.F.R.. (2020). Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability: Chemical prospection and bioactivity of cardoon seeds. in Food Chemistry
Elsevier Ltd., 323, 126838.
https://doi.org/10.1016/j.foodchem.2020.126838

Mandim F, Dias MI, Pinela J, Barracosa P, Ivanov M, Stojković D, Soković M, Santos-Buelga C, Barros L, Ferreira IC. Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability: Chemical prospection and bioactivity of cardoon seeds. in Food Chemistry. 2020;323:126838.
doi:10.1016/j.foodchem.2020.126838 .

Mandim, Filipa, Dias, Maria Inês, Pinela, José, Barracosa, Paulo, Ivanov, Marija, Stojković, Dejan, Soković, Marina, Santos-Buelga, Celestino, Barros, Lillian, Ferreira, Isabel C.F.R., "Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability: Chemical prospection and bioactivity of cardoon seeds" in Food Chemistry, 323 (2020):126838,
https://doi.org/10.1016/j.foodchem.2020.126838 . .