TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Fernandes, Ângela
AU  - Calhelha, Ricardo C.
AU  - Glamočlija, Jasmina
AU  - Barros, Lillian
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2023
UR  - https://www.mdpi.com/2309-608X/9/1/70
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9864295
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5461
AB  - Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).
PB  - Basel: MDPI
T2  - Journal of Fungi
T1  - A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity
IS  - 1
VL  - 9
DO  - 10.3390/jof9010070
SP  - 70
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Fernandes, Ângela and Calhelha, Ricardo C. and Glamočlija, Jasmina and Barros, Lillian and Soković, Marina and Ćirić, Ana",
year = "2023",
abstract = "Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).",
publisher = "Basel: MDPI",
journal = "Journal of Fungi",
title = "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity",
number = "1",
volume = "9",
doi = "10.3390/jof9010070",
pages = "70"
}

Kostić, M., Ivanov, M., Fernandes, Â., Calhelha, R. C., Glamočlija, J., Barros, L., Soković, M.,& Ćirić, A.. (2023). A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi
Basel: MDPI., 9(1), 70.
https://doi.org/10.3390/jof9010070

Kostić M, Ivanov M, Fernandes Â, Calhelha RC, Glamočlija J, Barros L, Soković M, Ćirić A. A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi. 2023;9(1):70.
doi:10.3390/jof9010070 .

Kostić, Marina, Ivanov, Marija, Fernandes, Ângela, Calhelha, Ricardo C., Glamočlija, Jasmina, Barros, Lillian, Soković, Marina, Ćirić, Ana, "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity" in Journal of Fungi, 9, no. 1 (2023):70,
https://doi.org/10.3390/jof9010070 . .

TY  - JOUR
AU  - Cavichi, Lucas Vinicius
AU  - Liberal, Ângela
AU  - Dias, Maria Inês
AU  - Mandim, Filipa
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Kalschne, Daneysa Lahis
AU  - Fernandes, Ângela
AU  - Canan, Cristiane
AU  - Barros, Lillian
AU  - Amaral, Joana S.
PY  - 2023
UR  - https://www.mdpi.com/2304-8158/12/1/192
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36613411
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9818490
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5400
AB  - In recent years, the interest in products of natural origin has boosted the exploitation and use of plants as food and sources of bioactive compounds, especially wild plants widely used in different cultures for several purposes. Commelina erecta is a wild edible plant (WEP) traditionally used as food and medicine, about which few studies exist. Thus, this study aimed at enhancing the knowledge about its nutritional, chemical and bioactive profile, considering different plant parts and development stages, in order to increase its inclusion in the diet of South American communities. The nutritional profile was found to be similar to other WEP frequently consumed in Brazil. Thirteen phenolic compounds (HPLC-DAD-ESI/MS) were tentatively identified, with apigenin, luteolin and quercetin derivatives being the most abundant. Fructose and oxalic acid were the major sugar and organic acid, respectively, in the aerial parts of C. erecta, and four isoforms of tocopherols were also identified. Regarding the plant's antioxidant activity, the EC50 values varied between 18.4 and 1060 µg/mL in the inhibition of lipid peroxidation assay (TBARS) and between 53 and 115 µg/mL in the oxidative haemolysis inhibition (OxHLIA) assay. The hydroethanolic extract obtained from stems at the flowering stage also presented anti-inflammatory activity. In general, all the extracts evidenced promising antimicrobial activity. Altogether, these results reinforce the traditional use of this plant species as food and medicine to support the diet of needier populations and also promote food sovereignty and sustainability.
PB  - Basel: MDPI
T2  - Foods
T1  - Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil
IS  - 1
VL  - 12
DO  - 10.3390/foods12010192
SP  - 192
ER  - 

@article{
author = "Cavichi, Lucas Vinicius and Liberal, Ângela and Dias, Maria Inês and Mandim, Filipa and Pinela, José and Kostić, Marina and Soković, Marina and Kalschne, Daneysa Lahis and Fernandes, Ângela and Canan, Cristiane and Barros, Lillian and Amaral, Joana S.",
year = "2023",
abstract = "In recent years, the interest in products of natural origin has boosted the exploitation and use of plants as food and sources of bioactive compounds, especially wild plants widely used in different cultures for several purposes. Commelina erecta is a wild edible plant (WEP) traditionally used as food and medicine, about which few studies exist. Thus, this study aimed at enhancing the knowledge about its nutritional, chemical and bioactive profile, considering different plant parts and development stages, in order to increase its inclusion in the diet of South American communities. The nutritional profile was found to be similar to other WEP frequently consumed in Brazil. Thirteen phenolic compounds (HPLC-DAD-ESI/MS) were tentatively identified, with apigenin, luteolin and quercetin derivatives being the most abundant. Fructose and oxalic acid were the major sugar and organic acid, respectively, in the aerial parts of C. erecta, and four isoforms of tocopherols were also identified. Regarding the plant's antioxidant activity, the EC50 values varied between 18.4 and 1060 µg/mL in the inhibition of lipid peroxidation assay (TBARS) and between 53 and 115 µg/mL in the oxidative haemolysis inhibition (OxHLIA) assay. The hydroethanolic extract obtained from stems at the flowering stage also presented anti-inflammatory activity. In general, all the extracts evidenced promising antimicrobial activity. Altogether, these results reinforce the traditional use of this plant species as food and medicine to support the diet of needier populations and also promote food sovereignty and sustainability.",
publisher = "Basel: MDPI",
journal = "Foods",
title = "Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil",
number = "1",
volume = "12",
doi = "10.3390/foods12010192",
pages = "192"
}

Cavichi, L. V., Liberal, Â., Dias, M. I., Mandim, F., Pinela, J., Kostić, M., Soković, M., Kalschne, D. L., Fernandes, Â., Canan, C., Barros, L.,& Amaral, J. S.. (2023). Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil. in Foods
Basel: MDPI., 12(1), 192.
https://doi.org/10.3390/foods12010192

Cavichi LV, Liberal Â, Dias MI, Mandim F, Pinela J, Kostić M, Soković M, Kalschne DL, Fernandes Â, Canan C, Barros L, Amaral JS. Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil. in Foods. 2023;12(1):192.
doi:10.3390/foods12010192 .

Cavichi, Lucas Vinicius, Liberal, Ângela, Dias, Maria Inês, Mandim, Filipa, Pinela, José, Kostić, Marina, Soković, Marina, Kalschne, Daneysa Lahis, Fernandes, Ângela, Canan, Cristiane, Barros, Lillian, Amaral, Joana S., "Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil" in Foods, 12, no. 1 (2023):192,
https://doi.org/10.3390/foods12010192 . .

TY  - JOUR
AU  - De Oliveira, Izamara
AU  - Chrysargyris, Antonios
AU  - Heleno, Sandrina A.
AU  - Carocho, Marcio
AU  - Calhelha, Ricardo
AU  - Dias, Maria Ines
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Petropoulos, Spyridon
AU  - Santos-Buelga, Celestino
AU  - Tzortzakis, Nikolaos
AU  - Barros, Lillian
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6389
AB  - The present study aims to determine the combined effect of cropping system and irrigation regime on the chemical composition and bioactive properties of lemon balm aerial parts. For this purpose, lemon balm plants were grown under two farming systems (conventional farming vs organic farming) and two irrigation levels (full irrigation vs deficit irrigation) and harvested twice throughout the growing period. The collected aerial parts were subjected to three different methods of extractions, namely infusion, maceration and ultrasound-assisted extraction and the obtained extracts were evaluated in terms of chemical profile and bioactivities. Five organic acids with varied composition among the tested treatments were identified in all the tested samples for both harvests, namely, citric, malic, oxalic, shikimic and quinic acid. Regarding phenolic compounds composition, the most abundant ones were rosmarinic acid, lithospermic acid A isomer I and hydroxylsalvianolic E, especially for the maceration and infusion extraction methods. Full irrigation resulted in lower EC50 values than deficit irrigation only in the treatments of the second harvest, while variable cytotoxic and anti-inflammatory effects were recorded in both harvests. Finally, in most cases the lemon balm extracts has similar or better activity than the positive controls, while the antifungal activity of lemon balm extracts was stronger than the antibacterial effects. In conclusion, the results of the present study showed that the implemented agronomic practices, as well as the extraction protocol may significantly affect the chemical profile and bioactivities of lemon balm extracts, suggesting that both the farming system and the irrigation schedule may improve the quality of the extracts depending on the implemented extraction protocol.
PB  - Elsevier
T2  - Food Research International
T1  - Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.
VL  - 170
DO  - 10.1016/j.foodres.2023.113044
SP  - 113044
ER  - 

@article{
author = "De Oliveira, Izamara and Chrysargyris, Antonios and Heleno, Sandrina A. and Carocho, Marcio and Calhelha, Ricardo and Dias, Maria Ines and Petrović, Jovana and Soković, Marina and Petropoulos, Spyridon and Santos-Buelga, Celestino and Tzortzakis, Nikolaos and Barros, Lillian",
year = "2023",
abstract = "The present study aims to determine the combined effect of cropping system and irrigation regime on the chemical composition and bioactive properties of lemon balm aerial parts. For this purpose, lemon balm plants were grown under two farming systems (conventional farming vs organic farming) and two irrigation levels (full irrigation vs deficit irrigation) and harvested twice throughout the growing period. The collected aerial parts were subjected to three different methods of extractions, namely infusion, maceration and ultrasound-assisted extraction and the obtained extracts were evaluated in terms of chemical profile and bioactivities. Five organic acids with varied composition among the tested treatments were identified in all the tested samples for both harvests, namely, citric, malic, oxalic, shikimic and quinic acid. Regarding phenolic compounds composition, the most abundant ones were rosmarinic acid, lithospermic acid A isomer I and hydroxylsalvianolic E, especially for the maceration and infusion extraction methods. Full irrigation resulted in lower EC50 values than deficit irrigation only in the treatments of the second harvest, while variable cytotoxic and anti-inflammatory effects were recorded in both harvests. Finally, in most cases the lemon balm extracts has similar or better activity than the positive controls, while the antifungal activity of lemon balm extracts was stronger than the antibacterial effects. In conclusion, the results of the present study showed that the implemented agronomic practices, as well as the extraction protocol may significantly affect the chemical profile and bioactivities of lemon balm extracts, suggesting that both the farming system and the irrigation schedule may improve the quality of the extracts depending on the implemented extraction protocol.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.",
volume = "170",
doi = "10.1016/j.foodres.2023.113044",
pages = "113044"
}

De Oliveira, I., Chrysargyris, A., Heleno, S. A., Carocho, M., Calhelha, R., Dias, M. I., Petrović, J., Soković, M., Petropoulos, S., Santos-Buelga, C., Tzortzakis, N.,& Barros, L.. (2023). Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.. in Food Research International
Elsevier., 170, 113044.
https://doi.org/10.1016/j.foodres.2023.113044

De Oliveira I, Chrysargyris A, Heleno SA, Carocho M, Calhelha R, Dias MI, Petrović J, Soković M, Petropoulos S, Santos-Buelga C, Tzortzakis N, Barros L. Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.. in Food Research International. 2023;170:113044.
doi:10.1016/j.foodres.2023.113044 .

De Oliveira, Izamara, Chrysargyris, Antonios, Heleno, Sandrina A., Carocho, Marcio, Calhelha, Ricardo, Dias, Maria Ines, Petrović, Jovana, Soković, Marina, Petropoulos, Spyridon, Santos-Buelga, Celestino, Tzortzakis, Nikolaos, Barros, Lillian, "Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes." in Food Research International, 170 (2023):113044,
https://doi.org/10.1016/j.foodres.2023.113044 . .

TY  - JOUR
AU  - Albuquerque, Bianca R.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Oliveira, M Beatriz P P
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0963996923001199
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5481
AB  - Chemical constituents and bioactive properties of rambutan (Nephelium lappaceum L.) peel were characterized and heat-/ultrasound-assisted extractions (HAE/UAE) of anthocyanins were optimized by response surface methodology. Five organic acids, the α-, γ-, and δ-tocopherol isoforms, and twenty-five fatty acids (36.8 % oleic acid) were identified, as well as a phenolic profile composed of ellagitannin derivatives, geraniin isomers, ellagic acid, and delphinidin-O derivatives. The extract showed antioxidant activity via lipid peroxidation (IC50 = 2.79 ± 0.03 µg/mL) and oxidative hemolysis (IC50 = 72 ± 2 µg/mL) inhibition, and displayed antibacterial and antifungal properties (MIC ≤ 1 mg/mL). On the other hand, no cytotoxicity was observed in tumor and non-tumor cell lines up to 400 µg/mL. The recovery of anthocyanins was more effective using HAE than UAE, allowing greater yields (16.2 mg/g extract) in just 3 min and using lower ethanol proportions. Overall, rambutan peel could be upcycled into bioactive ingredients and natural colorants for industrial applications.
T2  - Food Research International
T1  - Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins
VL  - 165
DO  - 10.1016/j.foodres.2023.112574
SP  - 112574
ER  - 

@article{
author = "Albuquerque, Bianca R. and Pinela, José and Dias, Maria Inês and Pereira, Carla and Petrović, Jovana and Soković, Marina and Calhelha, Ricardo C. and Oliveira, M Beatriz P P and Ferreira, Isabel C F R and Barros, Lillian",
year = "2023",
abstract = "Chemical constituents and bioactive properties of rambutan (Nephelium lappaceum L.) peel were characterized and heat-/ultrasound-assisted extractions (HAE/UAE) of anthocyanins were optimized by response surface methodology. Five organic acids, the α-, γ-, and δ-tocopherol isoforms, and twenty-five fatty acids (36.8 % oleic acid) were identified, as well as a phenolic profile composed of ellagitannin derivatives, geraniin isomers, ellagic acid, and delphinidin-O derivatives. The extract showed antioxidant activity via lipid peroxidation (IC50 = 2.79 ± 0.03 µg/mL) and oxidative hemolysis (IC50 = 72 ± 2 µg/mL) inhibition, and displayed antibacterial and antifungal properties (MIC ≤ 1 mg/mL). On the other hand, no cytotoxicity was observed in tumor and non-tumor cell lines up to 400 µg/mL. The recovery of anthocyanins was more effective using HAE than UAE, allowing greater yields (16.2 mg/g extract) in just 3 min and using lower ethanol proportions. Overall, rambutan peel could be upcycled into bioactive ingredients and natural colorants for industrial applications.",
journal = "Food Research International",
title = "Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins",
volume = "165",
doi = "10.1016/j.foodres.2023.112574",
pages = "112574"
}

Albuquerque, B. R., Pinela, J., Dias, M. I., Pereira, C., Petrović, J., Soković, M., Calhelha, R. C., Oliveira, M. B. P. P., Ferreira, I. C. F. R.,& Barros, L.. (2023). Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins. in Food Research International, 165, 112574.
https://doi.org/10.1016/j.foodres.2023.112574

Albuquerque BR, Pinela J, Dias MI, Pereira C, Petrović J, Soković M, Calhelha RC, Oliveira MBPP, Ferreira ICFR, Barros L. Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins. in Food Research International. 2023;165:112574.
doi:10.1016/j.foodres.2023.112574 .

Albuquerque, Bianca R., Pinela, José, Dias, Maria Inês, Pereira, Carla, Petrović, Jovana, Soković, Marina, Calhelha, Ricardo C., Oliveira, M Beatriz P P, Ferreira, Isabel C F R, Barros, Lillian, "Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins" in Food Research International, 165 (2023):112574,
https://doi.org/10.1016/j.foodres.2023.112574 . .

TY  - JOUR
AU  - Babota, Mihai
AU  - Frumuzachi, Oleg
AU  - Nicolescu, Alexandru
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Barros, Lillian
AU  - Añibarro-Ortega, Mikel
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Mocan, Andrei
AU  - López, Víctor
AU  - Crișan, Gianina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5475
AB  - Wild thyme aerial parts (Serpylli herba) are recognized as a valuable herbal product with antioxidant, anti-inflammatory, and antibacterial effects. Although pharmacopoeial regulations allow its collection exclusively from Thymus serpyllum, substitution with other species is frequent in current practice. This study analyzed the phenolic composition, antioxidant, and enzyme-inhibitory and antimicrobial activity of the hydroethanolic extracts obtained from five Romanian wild thyme species (Thymus alpestris, T. glabrescens, T. panonicus, T. pulcherimus and T. pulegioides). The analysis of individual phenolic constituents was performed through LC-ESI-DAD/MS2, while for the in vitro evaluation of antioxidant potential, TEAC, FRAP, DPPH, TBARS and OxHLIA assays were employed. The anti-enzymatic potential was tested in vitro against tyrosinase, α-glucosidase and acetylcholinesterase. High rosmarinic acid contents were quantified in all species (20.06 ± 0.32–80.49 ± 0.001 mg/g dry extract); phenolic acids derivatives (including salvianolic acids) were confirmed as the principal metabolites of T. alpestris and T. glabrescens, while eriodictyol-O-di-hexoside was found exclusively in T. alpestris. All species showed strong antioxidant potential and moderate anti-enzymatic effect against α-glucosidase and acetylcholinesterase, showing no anti-tyrosinase activity. This is the first detailed report on the chemical and biological profile of T. alpestris collected from Romanian spontaneous flora.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits
IS  - 2
VL  - 12
DO  - 10.3390/antiox12020390
SP  - 390
ER  - 

@article{
author = "Babota, Mihai and Frumuzachi, Oleg and Nicolescu, Alexandru and Dias, Maria Inês and Pinela, José and Barros, Lillian and Añibarro-Ortega, Mikel and Stojković, Dejan and Carević, Tamara and Mocan, Andrei and López, Víctor and Crișan, Gianina",
year = "2023",
abstract = "Wild thyme aerial parts (Serpylli herba) are recognized as a valuable herbal product with antioxidant, anti-inflammatory, and antibacterial effects. Although pharmacopoeial regulations allow its collection exclusively from Thymus serpyllum, substitution with other species is frequent in current practice. This study analyzed the phenolic composition, antioxidant, and enzyme-inhibitory and antimicrobial activity of the hydroethanolic extracts obtained from five Romanian wild thyme species (Thymus alpestris, T. glabrescens, T. panonicus, T. pulcherimus and T. pulegioides). The analysis of individual phenolic constituents was performed through LC-ESI-DAD/MS2, while for the in vitro evaluation of antioxidant potential, TEAC, FRAP, DPPH, TBARS and OxHLIA assays were employed. The anti-enzymatic potential was tested in vitro against tyrosinase, α-glucosidase and acetylcholinesterase. High rosmarinic acid contents were quantified in all species (20.06 ± 0.32–80.49 ± 0.001 mg/g dry extract); phenolic acids derivatives (including salvianolic acids) were confirmed as the principal metabolites of T. alpestris and T. glabrescens, while eriodictyol-O-di-hexoside was found exclusively in T. alpestris. All species showed strong antioxidant potential and moderate anti-enzymatic effect against α-glucosidase and acetylcholinesterase, showing no anti-tyrosinase activity. This is the first detailed report on the chemical and biological profile of T. alpestris collected from Romanian spontaneous flora.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits",
number = "2",
volume = "12",
doi = "10.3390/antiox12020390",
pages = "390"
}

Babota, M., Frumuzachi, O., Nicolescu, A., Dias, M. I., Pinela, J., Barros, L., Añibarro-Ortega, M., Stojković, D., Carević, T., Mocan, A., López, V.,& Crișan, G.. (2023). Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits. in Antioxidants
Basel: MDPI., 12(2), 390.
https://doi.org/10.3390/antiox12020390

Babota M, Frumuzachi O, Nicolescu A, Dias MI, Pinela J, Barros L, Añibarro-Ortega M, Stojković D, Carević T, Mocan A, López V, Crișan G. Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits. in Antioxidants. 2023;12(2):390.
doi:10.3390/antiox12020390 .

Babota, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Dias, Maria Inês, Pinela, José, Barros, Lillian, Añibarro-Ortega, Mikel, Stojković, Dejan, Carević, Tamara, Mocan, Andrei, López, Víctor, Crișan, Gianina, "Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits" in Antioxidants, 12, no. 2 (2023):390,
https://doi.org/10.3390/antiox12020390 . .

TY  - JOUR
AU  - da Silva, Tamires Barlati Vieira
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Mandim, Filipa
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Seixas, Flávio Augusto Vicente
AU  - Bracht, Adelar
AU  - Peralta, Rosane Marina
PY  - 2023
UR  - http://xlink.rsc.org/?DOI=D2FO02442J
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36723015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5465
AB  - A variety of the classic green tea plant, Camellia sinensis, was developed and is exclusive to Kenya. Due to high content of anthocyanin polyphenols in its leaves, the beverage obtained from this variety is purple in color and is the origin of the name purple tea. This work had two main purposes. The first one was to identify and quantify the major anthocyanin polyphenols in a hot water aqueous extract of the purple tea leaves. The second one was to test the hypothesis if this extract is capable of inhibiting triglyceride absorption considering that anthocyanin polyphenolics have been frequently associated to antilipidemic effects. Parallel experiments were always done with a similar green tea extract for comparison purposes. The antioxidant, anti-inflammatory, and cytotoxic activities of both tea varieties are similar. The purple tea extract, however, was strongly inhibitory toward the pancreatic lipase (minimal IC50 = 67.4 μg mL-1), whereas the green tea preparation was a weak inhibitor. Triglyceride digestion in mice was inhibited by the purple tea extract starting at 100 mg kg-1 dose and with a well-defined dose dependence. Green tea had no effect on triglyceride digestion at doses up to 500 mg kg-1. The latter effect is probably caused by several components in the purple tea extract including non-anthocyanin and anthocyanin polyphenols, the first ones acting solely via the inhibition of the pancreatic lipase and the latter by inhibiting both the lipase and the transport of free fatty acids from the intestinal lumen into the circulating blood. The results suggest that the regular consumption of Kenyan purple tea can be useful in the control of obesity.
T2  - Food & Function
T1  - Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice
IS  - 3
VL  - 14
DO  - 10.1039/d2fo02442j
SP  - 1761
EP  - 1772
ER  - 

@article{
author = "da Silva, Tamires Barlati Vieira and Dias, Maria Inês and Pereira, Carla and Mandim, Filipa and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian and Seixas, Flávio Augusto Vicente and Bracht, Adelar and Peralta, Rosane Marina",
year = "2023",
abstract = "A variety of the classic green tea plant, Camellia sinensis, was developed and is exclusive to Kenya. Due to high content of anthocyanin polyphenols in its leaves, the beverage obtained from this variety is purple in color and is the origin of the name purple tea. This work had two main purposes. The first one was to identify and quantify the major anthocyanin polyphenols in a hot water aqueous extract of the purple tea leaves. The second one was to test the hypothesis if this extract is capable of inhibiting triglyceride absorption considering that anthocyanin polyphenolics have been frequently associated to antilipidemic effects. Parallel experiments were always done with a similar green tea extract for comparison purposes. The antioxidant, anti-inflammatory, and cytotoxic activities of both tea varieties are similar. The purple tea extract, however, was strongly inhibitory toward the pancreatic lipase (minimal IC50 = 67.4 μg mL-1), whereas the green tea preparation was a weak inhibitor. Triglyceride digestion in mice was inhibited by the purple tea extract starting at 100 mg kg-1 dose and with a well-defined dose dependence. Green tea had no effect on triglyceride digestion at doses up to 500 mg kg-1. The latter effect is probably caused by several components in the purple tea extract including non-anthocyanin and anthocyanin polyphenols, the first ones acting solely via the inhibition of the pancreatic lipase and the latter by inhibiting both the lipase and the transport of free fatty acids from the intestinal lumen into the circulating blood. The results suggest that the regular consumption of Kenyan purple tea can be useful in the control of obesity.",
journal = "Food & Function",
title = "Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice",
number = "3",
volume = "14",
doi = "10.1039/d2fo02442j",
pages = "1761-1772"
}

da Silva, T. B. V., Dias, M. I., Pereira, C., Mandim, F., Ivanov, M., Soković, M., Ferreira, I. C. F. R., Barros, L., Seixas, F. A. V., Bracht, A.,& Peralta, R. M.. (2023). Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. in Food & Function, 14(3), 1761-1772.
https://doi.org/10.1039/d2fo02442j

da Silva TBV, Dias MI, Pereira C, Mandim F, Ivanov M, Soković M, Ferreira ICFR, Barros L, Seixas FAV, Bracht A, Peralta RM. Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. in Food & Function. 2023;14(3):1761-1772.
doi:10.1039/d2fo02442j .

da Silva, Tamires Barlati Vieira, Dias, Maria Inês, Pereira, Carla, Mandim, Filipa, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, Seixas, Flávio Augusto Vicente, Bracht, Adelar, Peralta, Rosane Marina, "Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice" in Food & Function, 14, no. 3 (2023):1761-1772,
https://doi.org/10.1039/d2fo02442j . .

TY  - JOUR
AU  - Garcia-Oliveira, Paula
AU  - Carreira-Casais, Anxo
AU  - Pereira, Eliana
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Simal-Gandara, Jesus
AU  - Prieto, Miguel A
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9571014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5170
AB  - Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.
PB  - Basel: MDPI
T2  - Molecules
T1  - From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants
IS  - 19
VL  - 27
DO  - 10.3390/molecules27196495
ER  - 

@article{
author = "Garcia-Oliveira, Paula and Carreira-Casais, Anxo and Pereira, Eliana and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C and Stojković, Dejan and Soković, Marina and Simal-Gandara, Jesus and Prieto, Miguel A and Caleja, Cristina and Barros, Lillian",
year = "2022",
abstract = "Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants",
number = "19",
volume = "27",
doi = "10.3390/molecules27196495"
}

Garcia-Oliveira, P., Carreira-Casais, A., Pereira, E., Dias, M. I., Pereira, C., Calhelha, R. C., Stojković, D., Soković, M., Simal-Gandara, J., Prieto, M. A., Caleja, C.,& Barros, L.. (2022). From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules
Basel: MDPI., 27(19).
https://doi.org/10.3390/molecules27196495

Garcia-Oliveira P, Carreira-Casais A, Pereira E, Dias MI, Pereira C, Calhelha RC, Stojković D, Soković M, Simal-Gandara J, Prieto MA, Caleja C, Barros L. From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules. 2022;27(19).
doi:10.3390/molecules27196495 .

Garcia-Oliveira, Paula, Carreira-Casais, Anxo, Pereira, Eliana, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C, Stojković, Dejan, Soković, Marina, Simal-Gandara, Jesus, Prieto, Miguel A, Caleja, Cristina, Barros, Lillian, "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants" in Molecules, 27, no. 19 (2022),
https://doi.org/10.3390/molecules27196495 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Chaski, Christina
AU  - Pereira, Carla
AU  - Kostić, Marina
AU  - Rouphael, Youssef
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Petropoulos, Spyridon A.
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/7/645
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5090
AB  - The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070645
SP  - 645
ER  - 

@article{
author = "Fernandes, Ângela and Chaski, Christina and Pereira, Carla and Kostić, Marina and Rouphael, Youssef and Soković, Marina and Barros, Lillian and Petropoulos, Spyridon A.",
year = "2022",
abstract = "The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070645",
pages = "645"
}

Fernandes, Â., Chaski, C., Pereira, C., Kostić, M., Rouphael, Y., Soković, M., Barros, L.,& Petropoulos, S. A.. (2022). Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae
Basel: MDPI., 8(7), 645.
https://doi.org/10.3390/horticulturae8070645

Fernandes Â, Chaski C, Pereira C, Kostić M, Rouphael Y, Soković M, Barros L, Petropoulos SA. Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae. 2022;8(7):645.
doi:10.3390/horticulturae8070645 .

Fernandes, Ângela, Chaski, Christina, Pereira, Carla, Kostić, Marina, Rouphael, Youssef, Soković, Marina, Barros, Lillian, Petropoulos, Spyridon A., "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit" in Horticulturae, 8, no. 7 (2022):645,
https://doi.org/10.3390/horticulturae8070645 . .

TY  - JOUR
AU  - Carocho, Márcio
AU  - Barros, Lillian
AU  - Morales, Patricia
AU  - Petropoulos, Spyridon A.
AU  - Soković, Marina
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.1077985/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5339
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Editorial: The chemistry of food in the advent of sustainable diets
VL  - 9
DO  - 10.3389/fnut.2022.1077985
SP  - 1077985
ER  - 

@article{
author = "Carocho, Márcio and Barros, Lillian and Morales, Patricia and Petropoulos, Spyridon A. and Soković, Marina",
year = "2022",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Editorial: The chemistry of food in the advent of sustainable diets",
volume = "9",
doi = "10.3389/fnut.2022.1077985",
pages = "1077985"
}

Carocho, M., Barros, L., Morales, P., Petropoulos, S. A.,& Soković, M.. (2022). Editorial: The chemistry of food in the advent of sustainable diets. in Frontiers in Nutrition
Lausanne: Frontiers Media S.A.., 9, 1077985.
https://doi.org/10.3389/fnut.2022.1077985

Carocho M, Barros L, Morales P, Petropoulos SA, Soković M. Editorial: The chemistry of food in the advent of sustainable diets. in Frontiers in Nutrition. 2022;9:1077985.
doi:10.3389/fnut.2022.1077985 .

Carocho, Márcio, Barros, Lillian, Morales, Patricia, Petropoulos, Spyridon A., Soković, Marina, "Editorial: The chemistry of food in the advent of sustainable diets" in Frontiers in Nutrition, 9 (2022):1077985,
https://doi.org/10.3389/fnut.2022.1077985 . .

TY  - CONF
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Caleja, Cristina
AU  - Pereira, Eliana
AU  - Barros, Lillian
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5156
AB  - Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Chemical composition and biological activity of two Lactarius species from Serbia.
SP  - 102
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5156
ER  - 

@conference{
author = "Kostić, Marina and Ivanov, Marija and Ćirić, Ana and Caleja, Cristina and Pereira, Eliana and Barros, Lillian and Stojković, Dejan and Soković, Marina and Glamočlija, Jasmina",
year = "2022",
abstract = "Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Chemical composition and biological activity of two Lactarius species from Serbia.",
pages = "102-103",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5156"
}

Kostić, M., Ivanov, M., Ćirić, A., Caleja, C., Pereira, E., Barros, L., Stojković, D., Soković, M.,& Glamočlija, J.. (2022). Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156

Kostić M, Ivanov M, Ćirić A, Caleja C, Pereira E, Barros L, Stojković D, Soković M, Glamočlija J. Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

Kostić, Marina, Ivanov, Marija, Ćirić, Ana, Caleja, Cristina, Pereira, Eliana, Barros, Lillian, Stojković, Dejan, Soković, Marina, Glamočlija, Jasmina, "Chemical composition and biological activity of two Lactarius species from Serbia." in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):102-103,
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Marković, Tatjana
AU  - Sanković Babić, Snežana
AU  - Barros, Lillian
AU  - Calhelha, Ricardo
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/10412905.2022.2107103
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5101
AB  - The chemical composition and biological activity of essential oils (EOs) from leaves of Origanum minutiflorum O. Schwarz & P.H.Davis, and seeds of Coriandrum sativum L. were investigated. Chemical analyses of EOs were performed and the major components were carvacrol (81.5%) and linalool (69.6%), respectively. The antimicrobial activity was assessed against several bacteria originating from the tonsillar tissue. Activities of EOs against Staphylococcus aureus biofilm were investigated, as well as the effect of the mixture of these EOs and antibiotics against the pathogen. The antioxidant activity of both EOs was determined by TBARS assay, and examined wild oregano EO showed better activity. Also, cytotoxicity of EOs was evaluated in vitro and both EOs showed potential to inhibit further proliferation of tumor cells. This study reported for the first time the effects of EOs on chronic tonsillitis causative pathogens, supporting their role as a natural bioactive therapeutic agent with possible antimicrobial applications.
PB  - Taylor and Francis Ltd.
T2  - Journal of Essential Oil Research
T1  - An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities
IS  - 6
VL  - 34
DO  - 10.1080/10412905.2022.2107103
SP  - 533
EP  - 543
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Marković, Tatjana and Sanković Babić, Snežana and Barros, Lillian and Calhelha, Ricardo and Soković, Marina and Ćirić, Ana",
year = "2022",
abstract = "The chemical composition and biological activity of essential oils (EOs) from leaves of Origanum minutiflorum O. Schwarz & P.H.Davis, and seeds of Coriandrum sativum L. were investigated. Chemical analyses of EOs were performed and the major components were carvacrol (81.5%) and linalool (69.6%), respectively. The antimicrobial activity was assessed against several bacteria originating from the tonsillar tissue. Activities of EOs against Staphylococcus aureus biofilm were investigated, as well as the effect of the mixture of these EOs and antibiotics against the pathogen. The antioxidant activity of both EOs was determined by TBARS assay, and examined wild oregano EO showed better activity. Also, cytotoxicity of EOs was evaluated in vitro and both EOs showed potential to inhibit further proliferation of tumor cells. This study reported for the first time the effects of EOs on chronic tonsillitis causative pathogens, supporting their role as a natural bioactive therapeutic agent with possible antimicrobial applications.",
publisher = "Taylor and Francis Ltd.",
journal = "Journal of Essential Oil Research",
title = "An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities",
number = "6",
volume = "34",
doi = "10.1080/10412905.2022.2107103",
pages = "533-543"
}

Kostić, M., Ivanov, M., Marković, T., Sanković Babić, S., Barros, L., Calhelha, R., Soković, M.,& Ćirić, A.. (2022). An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities. in Journal of Essential Oil Research
Taylor and Francis Ltd.., 34(6), 533-543.
https://doi.org/10.1080/10412905.2022.2107103

Kostić M, Ivanov M, Marković T, Sanković Babić S, Barros L, Calhelha R, Soković M, Ćirić A. An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities. in Journal of Essential Oil Research. 2022;34(6):533-543.
doi:10.1080/10412905.2022.2107103 .

Kostić, Marina, Ivanov, Marija, Marković, Tatjana, Sanković Babić, Snežana, Barros, Lillian, Calhelha, Ricardo, Soković, Marina, Ćirić, Ana, "An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities" in Journal of Essential Oil Research, 34, no. 6 (2022):533-543,
https://doi.org/10.1080/10412905.2022.2107103 . .

TY  - JOUR
AU  - Moreira, Bruna
AU  - Pereira, Eliana
AU  - Finimundy, Tiane C.
AU  - Pinela, José
AU  - Calhelha, Ricardo C.
AU  - Carocho, Márcio
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2022
UR  - http://xlink.rsc.org/?DOI=D2FO00657J
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5120
AB  - Pineapple is a tropical fruit consumed fresh or processed into various food products. However, the peel and crown of this fruit are not industrially exploited, thus generating tons of by-products that represent an economic and environmental concern. In order to promote the upcycling of these by-products, this work aimed to characterize the phenolic profile of its hydroethanolic extracts obtained from pineapple peel and crown leaves and to evaluate their in vitro bioactivity. The HPLC-DAD-ESI/MS analysis allowed the identification of 25 phenolic compounds, including phenolic acids and flavonoids. The antioxidant, cytotoxic, and antimicrobial activity assays highlighted the peel extract as the most promising and, therefore, it was incorporated into a traditional Portuguese pastry cake as a functional ingredient. The nutritional parameters of the developed food were not affected by the incorporation of the extract, but it promoted the antioxidant activity during its shelf-life. Overall, pineapple peel and crown appeared as promising by-products to be exploited by the food industry, which can be achieved through a circular economy approach.
PB  - Cambridge: Royal Society of Chemistry
T2  - Food & Function
T1  - Pineapple by-products as a source of bioactive compounds with potential for industrial food application.
IS  - 19
VL  - 13
DO  - 10.1039/d2fo00657j
SP  - 9959
EP  - 9972
ER  - 

@article{
author = "Moreira, Bruna and Pereira, Eliana and Finimundy, Tiane C. and Pinela, José and Calhelha, Ricardo C. and Carocho, Márcio and Stojković, Dejan and Soković, Marina and Ferreira, Isabel C. F. R. and Caleja, Cristina and Barros, Lillian",
year = "2022",
abstract = "Pineapple is a tropical fruit consumed fresh or processed into various food products. However, the peel and crown of this fruit are not industrially exploited, thus generating tons of by-products that represent an economic and environmental concern. In order to promote the upcycling of these by-products, this work aimed to characterize the phenolic profile of its hydroethanolic extracts obtained from pineapple peel and crown leaves and to evaluate their in vitro bioactivity. The HPLC-DAD-ESI/MS analysis allowed the identification of 25 phenolic compounds, including phenolic acids and flavonoids. The antioxidant, cytotoxic, and antimicrobial activity assays highlighted the peel extract as the most promising and, therefore, it was incorporated into a traditional Portuguese pastry cake as a functional ingredient. The nutritional parameters of the developed food were not affected by the incorporation of the extract, but it promoted the antioxidant activity during its shelf-life. Overall, pineapple peel and crown appeared as promising by-products to be exploited by the food industry, which can be achieved through a circular economy approach.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "Food & Function",
title = "Pineapple by-products as a source of bioactive compounds with potential for industrial food application.",
number = "19",
volume = "13",
doi = "10.1039/d2fo00657j",
pages = "9959-9972"
}

Moreira, B., Pereira, E., Finimundy, T. C., Pinela, J., Calhelha, R. C., Carocho, M., Stojković, D., Soković, M., Ferreira, I. C. F. R., Caleja, C.,& Barros, L.. (2022). Pineapple by-products as a source of bioactive compounds with potential for industrial food application.. in Food & Function
Cambridge: Royal Society of Chemistry., 13(19), 9959-9972.
https://doi.org/10.1039/d2fo00657j

Moreira B, Pereira E, Finimundy TC, Pinela J, Calhelha RC, Carocho M, Stojković D, Soković M, Ferreira ICFR, Caleja C, Barros L. Pineapple by-products as a source of bioactive compounds with potential for industrial food application.. in Food & Function. 2022;13(19):9959-9972.
doi:10.1039/d2fo00657j .

Moreira, Bruna, Pereira, Eliana, Finimundy, Tiane C., Pinela, José, Calhelha, Ricardo C., Carocho, Márcio, Stojković, Dejan, Soković, Marina, Ferreira, Isabel C. F. R., Caleja, Cristina, Barros, Lillian, "Pineapple by-products as a source of bioactive compounds with potential for industrial food application." in Food & Function, 13, no. 19 (2022):9959-9972,
https://doi.org/10.1039/d2fo00657j . .

TY  - JOUR
AU  - Besrour, Nermine
AU  - Oludemi, Taofiq
AU  - Mandim, Filipa
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Soković, Marina
AU  - Stojković, Dejan
AU  - Ferreira, Olga
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/4/677
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9031312
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4963
AB  - The cosmetic industry is constantly searching for bioactive ingredients, namely, those obtained from natural sources with environmentally friendly connotations and less toxic effects. A previous study of our research group optimized the extraction of phenolic compounds from Juglans regia by heat-assisted extraction. Due to its richness in different phenolic compounds, the present work aimed to develop a formulation containing J. regia leaf extract. The extract's antioxidant, anti-tyrosinase, antimicrobial, anti-inflammatory, wound healing, cytotoxicity, and photostability properties were evaluated. The extract was then incorporated into an O/W base cream, followed by characterization of the final formulation in terms of its antioxidant properties, phenolic composition, and stability over time and at different storage conditions. The most abundant compounds in the hydroethanolic extract were 3-O-caffeoylquinic acid (18.30 ± 0.04 mg/g), quercetin-O-pentoside (9.64 ± 0.06 mg/g), and quercetin 3-O-glucoside (6.70 ± 0.19 mg/g). Besides those, the extract presented antioxidant, anti-inflammatory, wound closure, and antibacterial effects against several skin pathogens. In addition, HaCaT cell viability was maintained up to 98% at 400 µg/mL. Within Proteus vulgaris-infected HaCaT cells, the extract also presented an over 40% bacterial mortality rate at its nontoxic concentration (200 µg/mL). After incorporating the extract, the obtained formulation presented a good physicochemical profile over time and at different storage conditions while also maintaining its antioxidant effect; as such, it can be considered stable for topical application. Future work to evaluate its performance in terms of skin permeation and detailed toxicological studies with a focus on regulatory requirements, involving skin irritation, eye irritation, genotoxicity, photo-irritation, and dermal absorption, should be conducted, as the prepared formulation demonstrated relevant properties that deserve to be further explored.
PB  - Basel: MDPI
T2  - Antioxidants (Basel, Switzerland)
T1  - Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.
IS  - 4
VL  - 11
DO  - 10.3390/antiox11040677
SP  - 677
ER  - 

@article{
author = "Besrour, Nermine and Oludemi, Taofiq and Mandim, Filipa and Pereira, Carla and Dias, Maria Inês and Soković, Marina and Stojković, Dejan and Ferreira, Olga and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2022",
abstract = "The cosmetic industry is constantly searching for bioactive ingredients, namely, those obtained from natural sources with environmentally friendly connotations and less toxic effects. A previous study of our research group optimized the extraction of phenolic compounds from Juglans regia by heat-assisted extraction. Due to its richness in different phenolic compounds, the present work aimed to develop a formulation containing J. regia leaf extract. The extract's antioxidant, anti-tyrosinase, antimicrobial, anti-inflammatory, wound healing, cytotoxicity, and photostability properties were evaluated. The extract was then incorporated into an O/W base cream, followed by characterization of the final formulation in terms of its antioxidant properties, phenolic composition, and stability over time and at different storage conditions. The most abundant compounds in the hydroethanolic extract were 3-O-caffeoylquinic acid (18.30 ± 0.04 mg/g), quercetin-O-pentoside (9.64 ± 0.06 mg/g), and quercetin 3-O-glucoside (6.70 ± 0.19 mg/g). Besides those, the extract presented antioxidant, anti-inflammatory, wound closure, and antibacterial effects against several skin pathogens. In addition, HaCaT cell viability was maintained up to 98% at 400 µg/mL. Within Proteus vulgaris-infected HaCaT cells, the extract also presented an over 40% bacterial mortality rate at its nontoxic concentration (200 µg/mL). After incorporating the extract, the obtained formulation presented a good physicochemical profile over time and at different storage conditions while also maintaining its antioxidant effect; as such, it can be considered stable for topical application. Future work to evaluate its performance in terms of skin permeation and detailed toxicological studies with a focus on regulatory requirements, involving skin irritation, eye irritation, genotoxicity, photo-irritation, and dermal absorption, should be conducted, as the prepared formulation demonstrated relevant properties that deserve to be further explored.",
publisher = "Basel: MDPI",
journal = "Antioxidants (Basel, Switzerland)",
title = "Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.",
number = "4",
volume = "11",
doi = "10.3390/antiox11040677",
pages = "677"
}

Besrour, N., Oludemi, T., Mandim, F., Pereira, C., Dias, M. I., Soković, M., Stojković, D., Ferreira, O., Ferreira, I. C. F. R.,& Barros, L.. (2022). Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.. in Antioxidants (Basel, Switzerland)
Basel: MDPI., 11(4), 677.
https://doi.org/10.3390/antiox11040677

Besrour N, Oludemi T, Mandim F, Pereira C, Dias MI, Soković M, Stojković D, Ferreira O, Ferreira ICFR, Barros L. Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.. in Antioxidants (Basel, Switzerland). 2022;11(4):677.
doi:10.3390/antiox11040677 .

Besrour, Nermine, Oludemi, Taofiq, Mandim, Filipa, Pereira, Carla, Dias, Maria Inês, Soković, Marina, Stojković, Dejan, Ferreira, Olga, Ferreira, Isabel C. F. R., Barros, Lillian, "Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development." in Antioxidants (Basel, Switzerland), 11, no. 4 (2022):677,
https://doi.org/10.3390/antiox11040677 . .

TY  - JOUR
AU  - Vieira, Dalila M.
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Barros, Lillian
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Barreiro, Maria Filomena
AU  - Ferreira, Isabel C.F.R.
AU  - Castro, Maria Cidalia R.
AU  - Rodrigues, Pedro V.
AU  - Machado, Ana Vera
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/fft2.141
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5435
AB  - Natural extracts have been used in several traditional medicine applications and culinary purposes. Their biological properties (antioxidant and antimicrobial) are due to the presence of several active aromatic compounds. Herein, different natural extracts were evaluated, namely structural and thermal characterization and biological activity, in its natural form and incorporated into a polymeric matrix, to assess their effective potential as additives for active food packaging. While rosemary presented the highest thermal stability with a degradation starting at 327°C, lemon balm extract was the less stable (180°C). Regarding the thiobarbituric acid assay, all extracts presented antioxidant activity, in oxidative hemolysis inhibition; anise, cinnamon, and clove extract did not present any action. Overall, the results demonstrated that leaves (rosemary and green tea) and the rhizome (curcumin) are the plant parts with the best performance. Therefore, extracts from aromatic plants are promising natural additives that can be incorporated into polymeric matrices to produce active food packaging film, increasing products shelf-life.
PB  - Hoboken: Wiley
T2  - Food Frontiers
T1  - Evaluation of plant extracts as an efficient source of additives for active food packaging
IS  - 3
VL  - 3
DO  - 10.1002/fft2.141
SP  - 480
EP  - 488
ER  - 

@article{
author = "Vieira, Dalila M. and Pereira, Carla and Calhelha, Ricardo C. and Barros, Lillian and Petrović, Jovana and Soković, Marina and Barreiro, Maria Filomena and Ferreira, Isabel C.F.R. and Castro, Maria Cidalia R. and Rodrigues, Pedro V. and Machado, Ana Vera",
year = "2022",
abstract = "Natural extracts have been used in several traditional medicine applications and culinary purposes. Their biological properties (antioxidant and antimicrobial) are due to the presence of several active aromatic compounds. Herein, different natural extracts were evaluated, namely structural and thermal characterization and biological activity, in its natural form and incorporated into a polymeric matrix, to assess their effective potential as additives for active food packaging. While rosemary presented the highest thermal stability with a degradation starting at 327°C, lemon balm extract was the less stable (180°C). Regarding the thiobarbituric acid assay, all extracts presented antioxidant activity, in oxidative hemolysis inhibition; anise, cinnamon, and clove extract did not present any action. Overall, the results demonstrated that leaves (rosemary and green tea) and the rhizome (curcumin) are the plant parts with the best performance. Therefore, extracts from aromatic plants are promising natural additives that can be incorporated into polymeric matrices to produce active food packaging film, increasing products shelf-life.",
publisher = "Hoboken: Wiley",
journal = "Food Frontiers",
title = "Evaluation of plant extracts as an efficient source of additives for active food packaging",
number = "3",
volume = "3",
doi = "10.1002/fft2.141",
pages = "480-488"
}

Vieira, D. M., Pereira, C., Calhelha, R. C., Barros, L., Petrović, J., Soković, M., Barreiro, M. F., Ferreira, I. C.F.R., Castro, M. C. R., Rodrigues, P. V.,& Machado, A. V.. (2022). Evaluation of plant extracts as an efficient source of additives for active food packaging. in Food Frontiers
Hoboken: Wiley., 3(3), 480-488.
https://doi.org/10.1002/fft2.141

Vieira DM, Pereira C, Calhelha RC, Barros L, Petrović J, Soković M, Barreiro MF, Ferreira IC, Castro MCR, Rodrigues PV, Machado AV. Evaluation of plant extracts as an efficient source of additives for active food packaging. in Food Frontiers. 2022;3(3):480-488.
doi:10.1002/fft2.141 .

Vieira, Dalila M., Pereira, Carla, Calhelha, Ricardo C., Barros, Lillian, Petrović, Jovana, Soković, Marina, Barreiro, Maria Filomena, Ferreira, Isabel C.F.R., Castro, Maria Cidalia R., Rodrigues, Pedro V., Machado, Ana Vera, "Evaluation of plant extracts as an efficient source of additives for active food packaging" in Food Frontiers, 3, no. 3 (2022):480-488,
https://doi.org/10.1002/fft2.141 . .

TY  - JOUR
AU  - Halabura, Marisangela Isabel Wietzikoski
AU  - Avelino, Katielle Vieira
AU  - Araújo, Nelma Lopes
AU  - Kassem, Adma Soraia Serea
AU  - Seixas, Flávio Augusto Vicente
AU  - Barros, Lillian
AU  - Fernandes, Ângela
AU  - Liberal, Ângela
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Linde, Giani Andrea
AU  - Colauto, Nelson Barros
AU  - do Valle, Juliana Silveira
PY  - 2022
UR  - https://link.springer.com/10.1007/s43630-022-00344-7
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5338
AB  - The mycelial biomass of basidiomycetes is a promising source of compounds and represents an alternative for industrial and biotechnological applications. Fungi use light as information and hold photoresponse mechanisms, in which sensors respond to light wavelengths and regulate various biological processes. Therefore, this study aimed to investigate the effects of blue, green, and red lights on the growth, chemical composition, and antioxidant and antimicrobial activity of Lentinus crinitus mycelial biomass. The chemical composition of the mycelial biomass was determined by chromatographic methods, antioxidant activity was analyzed by in vitro assays, and antimicrobial activity was investigated by the microdilution assay. The highest mycelial biomass yield was observed under blue-light cultivation. Many primordia arose under blue or green light, whereas the stroma was formed under red light. The presence of light altered the primary fungal metabolism, increasing the carbohydrate, tocopherol, fatty acid, and soluble sugar contents, mostly mannitol, and reducing the protein and organic acid concentrations. Cultivation under red light increased the phenol concentration. In contrast, cultivation under blue and green lights decreased phenol concentration. Benzoic and gallic acids were the main phenolic acids in the hydroalcoholic extracts, and the latter acids increased in all cultures under light, especially red light. Mycelial biomass cultivated under red light showed the highest antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ferric reducing antioxidant power (FRAP) method showed that all light wavelengths increased the antioxidant activity of mycelial biomass, with the highest value under red light. Moreover, the beta-carotene/linoleic acid co-oxidation (BCLA) assay demonstrated that the antioxidant activity was affected by light cultivation. Mycelial biomass grown under all conditions exhibited antibacterial and antifungal activities. Thus, mycelial biomass cultivation of L. crinitus under light conditions may be a promising strategy for controlling the mycelial chemical composition and biomass yield.
PB  - London: Springer Nature
T2  - Photochemical & Photobiological Sciences
T1  - Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass
DO  - 10.1007/s43630-022-00344-7
ER  - 

@article{
author = "Halabura, Marisangela Isabel Wietzikoski and Avelino, Katielle Vieira and Araújo, Nelma Lopes and Kassem, Adma Soraia Serea and Seixas, Flávio Augusto Vicente and Barros, Lillian and Fernandes, Ângela and Liberal, Ângela and Ivanov, Marija and Soković, Marina and Linde, Giani Andrea and Colauto, Nelson Barros and do Valle, Juliana Silveira",
year = "2022",
abstract = "The mycelial biomass of basidiomycetes is a promising source of compounds and represents an alternative for industrial and biotechnological applications. Fungi use light as information and hold photoresponse mechanisms, in which sensors respond to light wavelengths and regulate various biological processes. Therefore, this study aimed to investigate the effects of blue, green, and red lights on the growth, chemical composition, and antioxidant and antimicrobial activity of Lentinus crinitus mycelial biomass. The chemical composition of the mycelial biomass was determined by chromatographic methods, antioxidant activity was analyzed by in vitro assays, and antimicrobial activity was investigated by the microdilution assay. The highest mycelial biomass yield was observed under blue-light cultivation. Many primordia arose under blue or green light, whereas the stroma was formed under red light. The presence of light altered the primary fungal metabolism, increasing the carbohydrate, tocopherol, fatty acid, and soluble sugar contents, mostly mannitol, and reducing the protein and organic acid concentrations. Cultivation under red light increased the phenol concentration. In contrast, cultivation under blue and green lights decreased phenol concentration. Benzoic and gallic acids were the main phenolic acids in the hydroalcoholic extracts, and the latter acids increased in all cultures under light, especially red light. Mycelial biomass cultivated under red light showed the highest antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ferric reducing antioxidant power (FRAP) method showed that all light wavelengths increased the antioxidant activity of mycelial biomass, with the highest value under red light. Moreover, the beta-carotene/linoleic acid co-oxidation (BCLA) assay demonstrated that the antioxidant activity was affected by light cultivation. Mycelial biomass grown under all conditions exhibited antibacterial and antifungal activities. Thus, mycelial biomass cultivation of L. crinitus under light conditions may be a promising strategy for controlling the mycelial chemical composition and biomass yield.",
publisher = "London: Springer Nature",
journal = "Photochemical & Photobiological Sciences",
title = "Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass",
doi = "10.1007/s43630-022-00344-7"
}

Halabura, M. I. W., Avelino, K. V., Araújo, N. L., Kassem, A. S. S., Seixas, F. A. V., Barros, L., Fernandes, Â., Liberal, Â., Ivanov, M., Soković, M., Linde, G. A., Colauto, N. B.,& do Valle, J. S.. (2022). Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass. in Photochemical & Photobiological Sciences
London: Springer Nature..
https://doi.org/10.1007/s43630-022-00344-7

Halabura MIW, Avelino KV, Araújo NL, Kassem ASS, Seixas FAV, Barros L, Fernandes Â, Liberal Â, Ivanov M, Soković M, Linde GA, Colauto NB, do Valle JS. Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass. in Photochemical & Photobiological Sciences. 2022;.
doi:10.1007/s43630-022-00344-7 .

Halabura, Marisangela Isabel Wietzikoski, Avelino, Katielle Vieira, Araújo, Nelma Lopes, Kassem, Adma Soraia Serea, Seixas, Flávio Augusto Vicente, Barros, Lillian, Fernandes, Ângela, Liberal, Ângela, Ivanov, Marija, Soković, Marina, Linde, Giani Andrea, Colauto, Nelson Barros, do Valle, Juliana Silveira, "Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass" in Photochemical & Photobiological Sciences (2022),
https://doi.org/10.1007/s43630-022-00344-7 . .

TY  - JOUR
AU  - Babotă, Mihai
AU  - Frumuzachi, Oleg
AU  - Mocan, Andrei
AU  - Tămaș, Mircea
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Bădărău, Alexandru Sabin
AU  - Crișan, Gianina
AU  - Barros, Lillian
AU  - Păltinean, Ramona
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/20/2773
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5180
AB  - Hypericum perforatum L., also known as St. John’s Wort, is recognized worldwide as a valuable medicinal herb; however, other Hypericum species were intensively studied for their bioactive potential. To fill the research gap that exists in the scientific literature, a comparative evaluation between H. alpigenum Kit., H. perforatum L. and H. rochelii Griseb. & Schenk was conducted in the present study. Two types of herbal preparations obtained from the aerial parts of these species were analyzed: extracts obtained through maceration and extracts obtained through magnetic-stirring-assisted extraction. LC-DAD-ESI-MSn analysis revealed the presence of phenolic acids, flavan-3-ols and flavonoid derivatives as the main constituents of the above-mentioned species. Moreover, all extracts were tested for their antioxidant, enzyme-inhibitory and antimicrobial potential. Our work emphasizes for the first time a detailed description of H. rochelii phenolic fractions, including their phytochemical and bioactive characterization. In comparison with the other two studied species, H. rochelii was found as a rich source of phenolic acids and myricetin derivatives, showing important antioxidant, anticholinesterase and antibacterial activity. The study offers new perspectives regarding the chemical and bioactive profile of the less-studied species H. alpigenum and H. rochelii.
PB  - Basel: MDPI
T2  - Plants
T1  - Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii
IS  - 20
VL  - 11
DO  - 10.3390/plants11202773
SP  - 2773
ER  - 

@article{
author = "Babotă, Mihai and Frumuzachi, Oleg and Mocan, Andrei and Tămaș, Mircea and Dias, Maria Inês and Pinela, José and Stojković, Dejan and Soković, Marina and Bădărău, Alexandru Sabin and Crișan, Gianina and Barros, Lillian and Păltinean, Ramona",
year = "2022",
abstract = "Hypericum perforatum L., also known as St. John’s Wort, is recognized worldwide as a valuable medicinal herb; however, other Hypericum species were intensively studied for their bioactive potential. To fill the research gap that exists in the scientific literature, a comparative evaluation between H. alpigenum Kit., H. perforatum L. and H. rochelii Griseb. & Schenk was conducted in the present study. Two types of herbal preparations obtained from the aerial parts of these species were analyzed: extracts obtained through maceration and extracts obtained through magnetic-stirring-assisted extraction. LC-DAD-ESI-MSn analysis revealed the presence of phenolic acids, flavan-3-ols and flavonoid derivatives as the main constituents of the above-mentioned species. Moreover, all extracts were tested for their antioxidant, enzyme-inhibitory and antimicrobial potential. Our work emphasizes for the first time a detailed description of H. rochelii phenolic fractions, including their phytochemical and bioactive characterization. In comparison with the other two studied species, H. rochelii was found as a rich source of phenolic acids and myricetin derivatives, showing important antioxidant, anticholinesterase and antibacterial activity. The study offers new perspectives regarding the chemical and bioactive profile of the less-studied species H. alpigenum and H. rochelii.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii",
number = "20",
volume = "11",
doi = "10.3390/plants11202773",
pages = "2773"
}

Babotă, M., Frumuzachi, O., Mocan, A., Tămaș, M., Dias, M. I., Pinela, J., Stojković, D., Soković, M., Bădărău, A. S., Crișan, G., Barros, L.,& Păltinean, R.. (2022). Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii. in Plants
Basel: MDPI., 11(20), 2773.
https://doi.org/10.3390/plants11202773

Babotă M, Frumuzachi O, Mocan A, Tămaș M, Dias MI, Pinela J, Stojković D, Soković M, Bădărău AS, Crișan G, Barros L, Păltinean R. Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii. in Plants. 2022;11(20):2773.
doi:10.3390/plants11202773 .

Babotă, Mihai, Frumuzachi, Oleg, Mocan, Andrei, Tămaș, Mircea, Dias, Maria Inês, Pinela, José, Stojković, Dejan, Soković, Marina, Bădărău, Alexandru Sabin, Crișan, Gianina, Barros, Lillian, Păltinean, Ramona, "Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii" in Plants, 11, no. 20 (2022):2773,
https://doi.org/10.3390/plants11202773 . .

TY  - JOUR
AU  - Kissanga, Raquel
AU  - Liberal, Ângela
AU  - Diniz, Inês
AU  - Rodrigues, Ana S. B.
AU  - Baptista-Ferreira, João L.
AU  - Batista, Dora
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Fernandes, Ângela
AU  - Barros, Lillian
AU  - Catarino, Luís
PY  - 2022
UR  - https://www.mdpi.com/2304-8158/11/20/3240
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5179
AB  - The harvesting, processing, and sale of wild edible mushrooms (WEM) is a relevant economic activity in Angola and a good example of the use of non-wood forest products for food. Although there is deep traditional knowledge about the general properties of WEMs, a huge gap remains in detailed scientific knowledge. Thus, this study aimed to investigate the socio-economic importance of the species sold at local markets in Huila, Angola, from their molecular identification to the assessment of their nutritional, chemical, and bioactive profiles. From the eight WEM morphotypes studied, five were identified based on phenotypical and molecular approaches (four Russula spp., and Amanita loosei). The studied mushrooms proved to be a rich source of carbohydrates, proteins, and ashes, also presenting low amounts of fat. Chemical analyses further revealed mannitol as the main free sugar in all samples, and organic acids, namely, oxalic, quinic, malic, citric, and fumaric acids in low amounts. Additionally, the α-tocopherol isoform and monounsaturated fatty acids were predominant. Regarding phenolic acids, protocatechuic, p-hydroxybenzoic, p-coumaric, and cinnamic acids were detected in all mushroom hydroethanolic extracts, being responsible for their antioxidant, antibacterial, and antifungal activities. Our investigation contributes to the identification and knowledge of WEMs as important complementary food sources in Angola, some of which were reported for the first time, promoting their utilization as a basis of nutritional and functional ingredients, as being able to be part of a balanced diet and to be used in new bio-based formulations.
PB  - Basel: MDPI
T2  - Foods
T1  - Biochemical and Molecular Profiling of Wild Edible Mushrooms from Huila, Angola
IS  - 20
VL  - 11
DO  - 10.3390/foods11203240
SP  - 3240
ER  - 

@article{
author = "Kissanga, Raquel and Liberal, Ângela and Diniz, Inês and Rodrigues, Ana S. B. and Baptista-Ferreira, João L. and Batista, Dora and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Fernandes, Ângela and Barros, Lillian and Catarino, Luís",
year = "2022",
abstract = "The harvesting, processing, and sale of wild edible mushrooms (WEM) is a relevant economic activity in Angola and a good example of the use of non-wood forest products for food. Although there is deep traditional knowledge about the general properties of WEMs, a huge gap remains in detailed scientific knowledge. Thus, this study aimed to investigate the socio-economic importance of the species sold at local markets in Huila, Angola, from their molecular identification to the assessment of their nutritional, chemical, and bioactive profiles. From the eight WEM morphotypes studied, five were identified based on phenotypical and molecular approaches (four Russula spp., and Amanita loosei). The studied mushrooms proved to be a rich source of carbohydrates, proteins, and ashes, also presenting low amounts of fat. Chemical analyses further revealed mannitol as the main free sugar in all samples, and organic acids, namely, oxalic, quinic, malic, citric, and fumaric acids in low amounts. Additionally, the α-tocopherol isoform and monounsaturated fatty acids were predominant. Regarding phenolic acids, protocatechuic, p-hydroxybenzoic, p-coumaric, and cinnamic acids were detected in all mushroom hydroethanolic extracts, being responsible for their antioxidant, antibacterial, and antifungal activities. Our investigation contributes to the identification and knowledge of WEMs as important complementary food sources in Angola, some of which were reported for the first time, promoting their utilization as a basis of nutritional and functional ingredients, as being able to be part of a balanced diet and to be used in new bio-based formulations.",
publisher = "Basel: MDPI",
journal = "Foods",
title = "Biochemical and Molecular Profiling of Wild Edible Mushrooms from Huila, Angola",
number = "20",
volume = "11",
doi = "10.3390/foods11203240",
pages = "3240"
}

Kissanga, R., Liberal, Â., Diniz, I., Rodrigues, A. S. B., Baptista-Ferreira, J. L., Batista, D., Ivanov, M., Soković, M., Ferreira, I. C. F. R., Fernandes, Â., Barros, L.,& Catarino, L.. (2022). Biochemical and Molecular Profiling of Wild Edible Mushrooms from Huila, Angola. in Foods
Basel: MDPI., 11(20), 3240.
https://doi.org/10.3390/foods11203240

Kissanga R, Liberal Â, Diniz I, Rodrigues ASB, Baptista-Ferreira JL, Batista D, Ivanov M, Soković M, Ferreira ICFR, Fernandes Â, Barros L, Catarino L. Biochemical and Molecular Profiling of Wild Edible Mushrooms from Huila, Angola. in Foods. 2022;11(20):3240.
doi:10.3390/foods11203240 .

Kissanga, Raquel, Liberal, Ângela, Diniz, Inês, Rodrigues, Ana S. B., Baptista-Ferreira, João L., Batista, Dora, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Fernandes, Ângela, Barros, Lillian, Catarino, Luís, "Biochemical and Molecular Profiling of Wild Edible Mushrooms from Huila, Angola" in Foods, 11, no. 20 (2022):3240,
https://doi.org/10.3390/foods11203240 . .

TY  - JOUR
AU  - Othman, Souha
AU  - Añibarro-Ortega, Mikel
AU  - Dias, Maria Inês
AU  - Ćirić, Ana
AU  - Mandim, Filipa
AU  - Soković, Marina
AU  - Ferreira, Isabel C F R
AU  - Pinela, José
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9587281
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5175
AB  - Quince (Cydonia oblonga Mill.) is an astringent fruit widely processed into marmalade and other sweets through processes that discard the peel as a by-product. Therefore, this study was performed to characterize the quince peel composition in nutrients and phytochemicals and evaluate its in vitro biological activity, following a "zero waste" approach. The quince peel dry powder was particularly rich in fiber (20.2 g/100 g), fructose (34 g/100 g), malic acid (7.2 g/100 g), and potassium (692 mg/100 g). Extracts prepared by dynamic hydroethanolic maceration and hot water extraction yielded 4.70 and 4.27 mg/g of phenolic compounds, respectively, with a prevalence of flavan-3-ols. The hydroethanolic extract was the most effective in inhibiting lipid peroxidation and oxidative hemolysis, and also presented better antimicrobial effects against foodborne pathogens, which agreed with the highest flavan-3-ol contents. The extracts were better than control synthetic food additives against some tested fungal and bacterial strains. On the other hand, no ability to inhibit nitric oxide production or toxicity to the tumor and non-tumor cell lines was observed. Furthermore, the solid residues remaining after extraction contained 35-37 g/100 g of fiber. Overall, quince peel can be upcycled into fiber-rich and bioactive ingredients to endow the value chain with natural food fortifiers, preservatives, and health promoters.
PB  - Elsevier Ltd.
T2  - Heliyon
T1  - Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems
IS  - 10
VL  - 8
DO  - 10.1016/j.heliyon.2022.e11042
SP  - e11042
ER  - 

@article{
author = "Othman, Souha and Añibarro-Ortega, Mikel and Dias, Maria Inês and Ćirić, Ana and Mandim, Filipa and Soković, Marina and Ferreira, Isabel C F R and Pinela, José and Barros, Lillian",
year = "2022",
abstract = "Quince (Cydonia oblonga Mill.) is an astringent fruit widely processed into marmalade and other sweets through processes that discard the peel as a by-product. Therefore, this study was performed to characterize the quince peel composition in nutrients and phytochemicals and evaluate its in vitro biological activity, following a "zero waste" approach. The quince peel dry powder was particularly rich in fiber (20.2 g/100 g), fructose (34 g/100 g), malic acid (7.2 g/100 g), and potassium (692 mg/100 g). Extracts prepared by dynamic hydroethanolic maceration and hot water extraction yielded 4.70 and 4.27 mg/g of phenolic compounds, respectively, with a prevalence of flavan-3-ols. The hydroethanolic extract was the most effective in inhibiting lipid peroxidation and oxidative hemolysis, and also presented better antimicrobial effects against foodborne pathogens, which agreed with the highest flavan-3-ol contents. The extracts were better than control synthetic food additives against some tested fungal and bacterial strains. On the other hand, no ability to inhibit nitric oxide production or toxicity to the tumor and non-tumor cell lines was observed. Furthermore, the solid residues remaining after extraction contained 35-37 g/100 g of fiber. Overall, quince peel can be upcycled into fiber-rich and bioactive ingredients to endow the value chain with natural food fortifiers, preservatives, and health promoters.",
publisher = "Elsevier Ltd.",
journal = "Heliyon",
title = "Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems",
number = "10",
volume = "8",
doi = "10.1016/j.heliyon.2022.e11042",
pages = "e11042"
}

Othman, S., Añibarro-Ortega, M., Dias, M. I., Ćirić, A., Mandim, F., Soković, M., Ferreira, I. C. F. R., Pinela, J.,& Barros, L.. (2022). Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems. in Heliyon
Elsevier Ltd.., 8(10), e11042.
https://doi.org/10.1016/j.heliyon.2022.e11042

Othman S, Añibarro-Ortega M, Dias MI, Ćirić A, Mandim F, Soković M, Ferreira ICFR, Pinela J, Barros L. Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems. in Heliyon. 2022;8(10):e11042.
doi:10.1016/j.heliyon.2022.e11042 .

Othman, Souha, Añibarro-Ortega, Mikel, Dias, Maria Inês, Ćirić, Ana, Mandim, Filipa, Soković, Marina, Ferreira, Isabel C F R, Pinela, José, Barros, Lillian, "Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems" in Heliyon, 8, no. 10 (2022):e11042,
https://doi.org/10.1016/j.heliyon.2022.e11042 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Finimundy, Tiane C.
AU  - Polyzos, Nikolaos
AU  - Pinela, José
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/7/639
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5119
AB  - In the present study, the chemical profile and bioactive properties of the roots of turnip-rooted parsley (Petroselinum crispum spp. tuberosum) germplasm were evaluated. For this purpose, plants from seventeen parsley cultivars were grown in 6 L pots, and the obtained roots were analyzed in terms of nutritional value, chemical composition (tocopherols, sugars and organic and fatty acids) and bioactive content (antioxidant activity, phenolic compound composition and antimicrobial properties). Our results showed great variability in terms of the chemical composition and bioactive properties of root parsley germplasm. A higher fresh root yield was recorded for the common “Root parsley” common variety (164 g/pot), followed by the varieties “Osborne” (109 g/pot), “Sonata” (104 g/pot), “Kaśka” (104 g/pot) and “Halblange Berlinska” (103 g/pot), whereas the lowest yield was recorded for the “Hanacka” variety (69 g/pot). A significant variation was also observed in the nutritional value parameters: the roots of the “Sonata” genotype showed the highest fat content; “Arat”, “Osborne” and “Olomuńcka” had the highest ash content; the “Alba” cultivar contained significantly higher amounts of carbohydrates; and the “Vistula” cultivar showed the highest energetic value. The only detected isoforms of vitamin E were α- and δ-tocopherols; content varied depending on the cultivar, although α-tocopherol was the most abundant compound in most cultivars, especially in the “Arat” cultivar. Sucrose was the most abundant free sugar detected, especially in the “Sonata” cultivar (16.96 g/100 g dw), followed by apiose (2.93–5.55 g/100 g dw), glucose (1.3–3.47 g/100 g dw) and fructose (1.37–3.03 g/100 g dw). Moreover, malic acid was the most abundant organic acid in most of the tested cultivars. Twenty-one individual fatty acids were identified in all the studied cultivars, with linoleic (47.9–57.1%) and palmitic acid (20.66–20.5%) being the most abundant. Nineteen individual phenolic compounds were tentatively identified, including three phenolic acids, fourteen flavonoids and two hydrolyzable tannins, while apigenin-O-pentoside-O-hexoside was the most abundant. The antioxidant activity differed between the tested assays (TBARS and OxHLIA), and the most effective cultivars for the TBARS assay (“Root parsley (common variety)” and “Berlinski Halblange Springer”) were those with the lowest antioxidant activity for the OxHLIA assay after 120 min. Finally, in most cases, the root extracts were more efficient or similarly effective compared to the positive controls against the tested bacteria and fungi. In conclusion, our results provide information regarding the chemical characterization and the bioactivities of the roots of turnip-rooted parsley germplasm that could be further exploited in sustainable and diversified agro-ecosystems through the introduction of this species as a novel/complementary crop in the traditional farming systems of the Mediterranean basin.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070639
SP  - 639
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Finimundy, Tiane C. and Polyzos, Nikolaos and Pinela, José and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2022",
abstract = "In the present study, the chemical profile and bioactive properties of the roots of turnip-rooted parsley (Petroselinum crispum spp. tuberosum) germplasm were evaluated. For this purpose, plants from seventeen parsley cultivars were grown in 6 L pots, and the obtained roots were analyzed in terms of nutritional value, chemical composition (tocopherols, sugars and organic and fatty acids) and bioactive content (antioxidant activity, phenolic compound composition and antimicrobial properties). Our results showed great variability in terms of the chemical composition and bioactive properties of root parsley germplasm. A higher fresh root yield was recorded for the common “Root parsley” common variety (164 g/pot), followed by the varieties “Osborne” (109 g/pot), “Sonata” (104 g/pot), “Kaśka” (104 g/pot) and “Halblange Berlinska” (103 g/pot), whereas the lowest yield was recorded for the “Hanacka” variety (69 g/pot). A significant variation was also observed in the nutritional value parameters: the roots of the “Sonata” genotype showed the highest fat content; “Arat”, “Osborne” and “Olomuńcka” had the highest ash content; the “Alba” cultivar contained significantly higher amounts of carbohydrates; and the “Vistula” cultivar showed the highest energetic value. The only detected isoforms of vitamin E were α- and δ-tocopherols; content varied depending on the cultivar, although α-tocopherol was the most abundant compound in most cultivars, especially in the “Arat” cultivar. Sucrose was the most abundant free sugar detected, especially in the “Sonata” cultivar (16.96 g/100 g dw), followed by apiose (2.93–5.55 g/100 g dw), glucose (1.3–3.47 g/100 g dw) and fructose (1.37–3.03 g/100 g dw). Moreover, malic acid was the most abundant organic acid in most of the tested cultivars. Twenty-one individual fatty acids were identified in all the studied cultivars, with linoleic (47.9–57.1%) and palmitic acid (20.66–20.5%) being the most abundant. Nineteen individual phenolic compounds were tentatively identified, including three phenolic acids, fourteen flavonoids and two hydrolyzable tannins, while apigenin-O-pentoside-O-hexoside was the most abundant. The antioxidant activity differed between the tested assays (TBARS and OxHLIA), and the most effective cultivars for the TBARS assay (“Root parsley (common variety)” and “Berlinski Halblange Springer”) were those with the lowest antioxidant activity for the OxHLIA assay after 120 min. Finally, in most cases, the root extracts were more efficient or similarly effective compared to the positive controls against the tested bacteria and fungi. In conclusion, our results provide information regarding the chemical characterization and the bioactivities of the roots of turnip-rooted parsley germplasm that could be further exploited in sustainable and diversified agro-ecosystems through the introduction of this species as a novel/complementary crop in the traditional farming systems of the Mediterranean basin.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070639",
pages = "639"
}

Petropoulos, S. A., Fernandes, Â., Finimundy, T. C., Polyzos, N., Pinela, J., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2022). The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm. in Horticulturae
Basel: MDPI., 8(7), 639.
https://doi.org/10.3390/horticulturae8070639

Petropoulos SA, Fernandes Â, Finimundy TC, Polyzos N, Pinela J, Ivanov M, Soković M, Ferreira ICFR, Barros L. The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm. in Horticulturae. 2022;8(7):639.
doi:10.3390/horticulturae8070639 .

Petropoulos, Spyridon A., Fernandes, Ângela, Finimundy, Tiane C., Polyzos, Nikolaos, Pinela, José, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm" in Horticulturae, 8, no. 7 (2022):639,
https://doi.org/10.3390/horticulturae8070639 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Santos-Buelga, Celestino
AU  - Barros, Lillian
PY  - 2022
UR  - https://www.mdpi.com/2079-7737/11/5/699
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4980
AB  - Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1-B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O-caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 µg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 µg/mL for Δt of 60 and 120 min, respectively) and higher cytotoxic (B8-B13, GI50 between 7.1 and 17 µg/mL), anti-inflammatory (B13, IC50 of 10 µg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others.
PB  - Basel: MDPI
T2  - Biology
T1  - Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.
IS  - 5
VL  - 11
DO  - 10.3390/biology11050699
SP  - 699
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Pinela, José and Dias, Maria Inês and Kostić, Marina and Soković, Marina and Ferreira, Isabel C. F. R. and Santos-Buelga, Celestino and Barros, Lillian",
year = "2022",
abstract = "Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1-B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O-caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 µg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 µg/mL for Δt of 60 and 120 min, respectively) and higher cytotoxic (B8-B13, GI50 between 7.1 and 17 µg/mL), anti-inflammatory (B13, IC50 of 10 µg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others.",
publisher = "Basel: MDPI",
journal = "Biology",
title = "Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.",
number = "5",
volume = "11",
doi = "10.3390/biology11050699",
pages = "699"
}

Mandim, F., Petropoulos, S. A., Pinela, J., Dias, M. I., Kostić, M., Soković, M., Ferreira, I. C. F. R., Santos-Buelga, C.,& Barros, L.. (2022). Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.. in Biology
Basel: MDPI., 11(5), 699.
https://doi.org/10.3390/biology11050699

Mandim F, Petropoulos SA, Pinela J, Dias MI, Kostić M, Soković M, Ferreira ICFR, Santos-Buelga C, Barros L. Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.. in Biology. 2022;11(5):699.
doi:10.3390/biology11050699 .

Mandim, Filipa, Petropoulos, Spyridon A., Pinela, José, Dias, Maria Inês, Kostić, Marina, Soković, Marina, Ferreira, Isabel C. F. R., Santos-Buelga, Celestino, Barros, Lillian, "Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages." in Biology, 11, no. 5 (2022):699,
https://doi.org/10.3390/biology11050699 . .

TY  - JOUR
AU  - Bertéli, Míria Benetati Delgado
AU  - de Souza, Mariana Martins Magalhães
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Dragunski, Douglas Cardoso
AU  - do Valle, Juliana Silveira
AU  - de Souza Ferreira, Ederlan
AU  - Pinto, Laise Cedraz
AU  - de Souza, Carolina Oliveira
AU  - Ruiz, Suelen Pereira
AU  - Linde, Giani Andrea
AU  - Colauto, Nelson Barros
PY  - 2022
UR  - https://link.springer.com/10.1007/s11274-022-03257-w
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4943
AB  - Lentinus crinitus basidiocarps are an alternative to antimicrobials, but the stipe (24% basidiocarp) is discarded even with potential antimicrobial activity. This study evaluated the antimicrobial activity of L. crinitus basidiocarp pileus and stipe extracts against foodborne pathogens and food spoilage microorganisms. Basidiocarps of L. crinitus were grown in sugarcane bagasse and rice husks and the pileus and stipe methanolic extract was analyzed by broth microdilution method for antimicrobial activity against eight bacteria and eight fungi. The minimum bactericidal concentration values for pileus and stipe ranged from 0.40 to 0.50 mg mL- 1, for streptomycin from 0.10 to 0.50 mg mL- 1, and for ampicillin from 0.40 to 1.20 mg mL- 1. The minimum fungicidal concentration values for pileus and stipe ranged from 0.06 to 0.60 mg mL- 1, for bifonazole from 0.20 to 0.25 mg mL- 1, and for ketoconazole from 0.30 to 3.50 mg mL- 1. Extracts had bacteriostatic, bactericidal, fungistatic and fungicidal activity against all microorganisms, but with greater efficiency and specificity for some microorganisms. Both pileus and stipe are promising and sustainable alternatives for use in food, agricultural, and pharmaceutical industries.
T2  - World Journal of Microbiology and Biotechnology
T1  - Basidiocarp structures of Lentinus crinitus: an antimicrobial source against foodborne pathogens and food spoilage microorganisms.
IS  - 5
VL  - 38
DO  - 10.1007/s11274-022-03257-w
SP  - 74
ER  - 

@article{
author = "Bertéli, Míria Benetati Delgado and de Souza, Mariana Martins Magalhães and Barros, Lillian and Ferreira, Isabel C. F. R. and Glamočlija, Jasmina and Soković, Marina and Dragunski, Douglas Cardoso and do Valle, Juliana Silveira and de Souza Ferreira, Ederlan and Pinto, Laise Cedraz and de Souza, Carolina Oliveira and Ruiz, Suelen Pereira and Linde, Giani Andrea and Colauto, Nelson Barros",
year = "2022",
abstract = "Lentinus crinitus basidiocarps are an alternative to antimicrobials, but the stipe (24% basidiocarp) is discarded even with potential antimicrobial activity. This study evaluated the antimicrobial activity of L. crinitus basidiocarp pileus and stipe extracts against foodborne pathogens and food spoilage microorganisms. Basidiocarps of L. crinitus were grown in sugarcane bagasse and rice husks and the pileus and stipe methanolic extract was analyzed by broth microdilution method for antimicrobial activity against eight bacteria and eight fungi. The minimum bactericidal concentration values for pileus and stipe ranged from 0.40 to 0.50 mg mL- 1, for streptomycin from 0.10 to 0.50 mg mL- 1, and for ampicillin from 0.40 to 1.20 mg mL- 1. The minimum fungicidal concentration values for pileus and stipe ranged from 0.06 to 0.60 mg mL- 1, for bifonazole from 0.20 to 0.25 mg mL- 1, and for ketoconazole from 0.30 to 3.50 mg mL- 1. Extracts had bacteriostatic, bactericidal, fungistatic and fungicidal activity against all microorganisms, but with greater efficiency and specificity for some microorganisms. Both pileus and stipe are promising and sustainable alternatives for use in food, agricultural, and pharmaceutical industries.",
journal = "World Journal of Microbiology and Biotechnology",
title = "Basidiocarp structures of Lentinus crinitus: an antimicrobial source against foodborne pathogens and food spoilage microorganisms.",
number = "5",
volume = "38",
doi = "10.1007/s11274-022-03257-w",
pages = "74"
}

Bertéli, M. B. D., de Souza, M. M. M., Barros, L., Ferreira, I. C. F. R., Glamočlija, J., Soković, M., Dragunski, D. C., do Valle, J. S., de Souza Ferreira, E., Pinto, L. C., de Souza, C. O., Ruiz, S. P., Linde, G. A.,& Colauto, N. B.. (2022). Basidiocarp structures of Lentinus crinitus: an antimicrobial source against foodborne pathogens and food spoilage microorganisms.. in World Journal of Microbiology and Biotechnology, 38(5), 74.
https://doi.org/10.1007/s11274-022-03257-w

Bertéli MBD, de Souza MMM, Barros L, Ferreira ICFR, Glamočlija J, Soković M, Dragunski DC, do Valle JS, de Souza Ferreira E, Pinto LC, de Souza CO, Ruiz SP, Linde GA, Colauto NB. Basidiocarp structures of Lentinus crinitus: an antimicrobial source against foodborne pathogens and food spoilage microorganisms.. in World Journal of Microbiology and Biotechnology. 2022;38(5):74.
doi:10.1007/s11274-022-03257-w .

Bertéli, Míria Benetati Delgado, de Souza, Mariana Martins Magalhães, Barros, Lillian, Ferreira, Isabel C. F. R., Glamočlija, Jasmina, Soković, Marina, Dragunski, Douglas Cardoso, do Valle, Juliana Silveira, de Souza Ferreira, Ederlan, Pinto, Laise Cedraz, de Souza, Carolina Oliveira, Ruiz, Suelen Pereira, Linde, Giani Andrea, Colauto, Nelson Barros, "Basidiocarp structures of Lentinus crinitus: an antimicrobial source against foodborne pathogens and food spoilage microorganisms." in World Journal of Microbiology and Biotechnology, 38, no. 5 (2022):74,
https://doi.org/10.1007/s11274-022-03257-w . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Dias, Maria Inês
AU  - Zengin, Gokhan
AU  - Barros, Lillian
AU  - Ivanov, Marija
AU  - Gašić, Uroš
AU  - Rajčević, Nemanja
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C.F.R.
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4871
AB  - Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa extract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic effect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.
PB  - Dortmund: EXCLI Journal
T2  - EXCLI Journal
T1  - Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line
VL  - 21
DO  - 10.17179/excli2021-4487
SP  - 387
EP  - 399
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Dias, Maria Inês and Zengin, Gokhan and Barros, Lillian and Ivanov, Marija and Gašić, Uroš and Rajčević, Nemanja and Stevanović, Milena and Ferreira, Isabel C.F.R. and Soković, Marina",
year = "2022",
abstract = "Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa extract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic effect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.",
publisher = "Dortmund: EXCLI Journal",
journal = "EXCLI Journal",
title = "Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line",
volume = "21",
doi = "10.17179/excli2021-4487",
pages = "387-399"
}

Stojković, D., Drakulić, D., Dias, M. I., Zengin, G., Barros, L., Ivanov, M., Gašić, U., Rajčević, N., Stevanović, M., Ferreira, I. C.F.R.,& Soković, M.. (2022). Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line. in EXCLI Journal
Dortmund: EXCLI Journal., 21, 387-399.
https://doi.org/10.17179/excli2021-4487

Stojković D, Drakulić D, Dias MI, Zengin G, Barros L, Ivanov M, Gašić U, Rajčević N, Stevanović M, Ferreira IC, Soković M. Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line. in EXCLI Journal. 2022;21:387-399.
doi:10.17179/excli2021-4487 .

Stojković, Dejan, Drakulić, Danijela, Dias, Maria Inês, Zengin, Gokhan, Barros, Lillian, Ivanov, Marija, Gašić, Uroš, Rajčević, Nemanja, Stevanović, Milena, Ferreira, Isabel C.F.R., Soković, Marina, "Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line" in EXCLI Journal, 21 (2022):387-399,
https://doi.org/10.17179/excli2021-4487 . .

TY  - JOUR
AU  - Silva, Ana Rita
AU  - Ayuso, Manuel
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - García, Pablo A.
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669021011080
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4746
AB  - Cytinus hypocistis (L.) L. is a comestible holoparasite with great potential for cosmeceutical application. Although its high tannin content has been associated with its bioactive and inhibitory enzyme properties, this is the first report establishing a relationship between parasite and host (Halimium lasianthum (Lam.) Greuter) phenolic profile and bioactive properties. Thus, five extracts (aerial and root extracts of non-parasited and parasited H. lasianthum and C. hypocistis) were evaluated. The tentative identification of both species comprises 39 phenolic compounds. Hydrolysable tannins and flavonoids were the main identified groups in C. hypocistis and H. lasianthum extracts, respectively. Regarding bioactivities, C. hypocistis exhibited excellent antioxidant results both in Oxidative Haemolysis (OxHLIA) and inhibition of Thiobarbituric Acid Reactive Substances Formation (TBARS). The tested extracts presented antimicrobial inhibition, anti-inflammatory activity, and effective cytotoxicity against tumour cells. C. hypocistis exhibited the lowest cytotoxicity on a non-tumour cell line. Principal Component Analysis (PCA) was a suitable approach to analyse differences among samples, explaining up to 67% of data variability and suggesting no similarities between parasite and host phenolic composition and bioactivities. Therefore, this comparative study emphasises the significance of both species as a source of biologically active compounds.
PB  - Amsterdam: Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter
VL  - 176
DO  - 10.1016/j.indcrop.2021.114343
SP  - 114343
ER  - 

@article{
author = "Silva, Ana Rita and Ayuso, Manuel and Pereira, Carla and Dias, Maria Inês and Kostić, Marina and Calhelha, Ricardo C. and Soković, Marina and García, Pablo A. and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2022",
abstract = "Cytinus hypocistis (L.) L. is a comestible holoparasite with great potential for cosmeceutical application. Although its high tannin content has been associated with its bioactive and inhibitory enzyme properties, this is the first report establishing a relationship between parasite and host (Halimium lasianthum (Lam.) Greuter) phenolic profile and bioactive properties. Thus, five extracts (aerial and root extracts of non-parasited and parasited H. lasianthum and C. hypocistis) were evaluated. The tentative identification of both species comprises 39 phenolic compounds. Hydrolysable tannins and flavonoids were the main identified groups in C. hypocistis and H. lasianthum extracts, respectively. Regarding bioactivities, C. hypocistis exhibited excellent antioxidant results both in Oxidative Haemolysis (OxHLIA) and inhibition of Thiobarbituric Acid Reactive Substances Formation (TBARS). The tested extracts presented antimicrobial inhibition, anti-inflammatory activity, and effective cytotoxicity against tumour cells. C. hypocistis exhibited the lowest cytotoxicity on a non-tumour cell line. Principal Component Analysis (PCA) was a suitable approach to analyse differences among samples, explaining up to 67% of data variability and suggesting no similarities between parasite and host phenolic composition and bioactivities. Therefore, this comparative study emphasises the significance of both species as a source of biologically active compounds.",
publisher = "Amsterdam: Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter",
volume = "176",
doi = "10.1016/j.indcrop.2021.114343",
pages = "114343"
}

Silva, A. R., Ayuso, M., Pereira, C., Dias, M. I., Kostić, M., Calhelha, R. C., Soković, M., García, P. A., Ferreira, I. C.F.R.,& Barros, L.. (2022). Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter. in Industrial Crops and Products
Amsterdam: Elsevier B.V.., 176, 114343.
https://doi.org/10.1016/j.indcrop.2021.114343

Silva AR, Ayuso M, Pereira C, Dias MI, Kostić M, Calhelha RC, Soković M, García PA, Ferreira IC, Barros L. Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter. in Industrial Crops and Products. 2022;176:114343.
doi:10.1016/j.indcrop.2021.114343 .

Silva, Ana Rita, Ayuso, Manuel, Pereira, Carla, Dias, Maria Inês, Kostić, Marina, Calhelha, Ricardo C., Soković, Marina, García, Pablo A., Ferreira, Isabel C.F.R., Barros, Lillian, "Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter" in Industrial Crops and Products, 176 (2022):114343,
https://doi.org/10.1016/j.indcrop.2021.114343 . .

TY  - CONF
AU  - Stojković, Dejan
AU  - Kostić, Marina
AU  - Fernandes, Angela
AU  - Barros, Lillian
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5157
AB  - Craterellus cornucopioides (L.) Pers. (Cantharellaceae), or horn of plenty, is an edible fungi. It is also known as the black chanterelle, black trumpet, crna truba (Serbian), trompette de la mort (French), trombetta dei morti (Italian) or trumpet of the dead, djondjon (Haitian). C. cornucopioides growing wild in Serbia was chemically characterized in nutritional value and individual compounds (sugars, fatty acids, tocopherols, organic acids and phenolic compounds). Antioxidant (scavenging activity, reducing power and inhibition of lipid peroxidation), antibacterial and antifungal activities of its methanolic extract were evaluated under in vitro conditions. C. cornucopioides was showed to be rich in carbohydrates (79.32 g/100g dw), followed by proteins (8.36 g/100g dw), ash (9.43 g/100g dw) and fat (2.90 g/100g dw). The most abundant sugar compound was identified as mannitol (14.16 g/100g dw); four tocopherol isoforms (α-, β-,γ- and δ-tocopherols) were detected. Unsaturated fatty acids predominated over saturated fatty acids, with oleic and linoleic acids as the most dominant ones. Five organic acids were quantified: oxalic, citric, quinic, and fumaric and malic acids. The methanolic extract of the mushroom showed in vitro antioxidant and antimicrobial activities. Methanolic extract successively inhibited growth of pathogenic bacteria.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides
SP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5157
ER  - 

@conference{
author = "Stojković, Dejan and Kostić, Marina and Fernandes, Angela and Barros, Lillian and Glamočlija, Jasmina",
year = "2022",
abstract = "Craterellus cornucopioides (L.) Pers. (Cantharellaceae), or horn of plenty, is an edible fungi. It is also known as the black chanterelle, black trumpet, crna truba (Serbian), trompette de la mort (French), trombetta dei morti (Italian) or trumpet of the dead, djondjon (Haitian). C. cornucopioides growing wild in Serbia was chemically characterized in nutritional value and individual compounds (sugars, fatty acids, tocopherols, organic acids and phenolic compounds). Antioxidant (scavenging activity, reducing power and inhibition of lipid peroxidation), antibacterial and antifungal activities of its methanolic extract were evaluated under in vitro conditions. C. cornucopioides was showed to be rich in carbohydrates (79.32 g/100g dw), followed by proteins (8.36 g/100g dw), ash (9.43 g/100g dw) and fat (2.90 g/100g dw). The most abundant sugar compound was identified as mannitol (14.16 g/100g dw); four tocopherol isoforms (α-, β-,γ- and δ-tocopherols) were detected. Unsaturated fatty acids predominated over saturated fatty acids, with oleic and linoleic acids as the most dominant ones. Five organic acids were quantified: oxalic, citric, quinic, and fumaric and malic acids. The methanolic extract of the mushroom showed in vitro antioxidant and antimicrobial activities. Methanolic extract successively inhibited growth of pathogenic bacteria.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides",
pages = "168",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5157"
}

Stojković, D., Kostić, M., Fernandes, A., Barros, L.,& Glamočlija, J.. (2022). Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 168.
https://hdl.handle.net/21.15107/rcub_ibiss_5157

Stojković D, Kostić M, Fernandes A, Barros L, Glamočlija J. Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:168.
https://hdl.handle.net/21.15107/rcub_ibiss_5157 .

Stojković, Dejan, Kostić, Marina, Fernandes, Angela, Barros, Lillian, Glamočlija, Jasmina, "Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides" in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):168,
https://hdl.handle.net/21.15107/rcub_ibiss_5157 .

TY  - JOUR
AU  - Mocan, Andrei
AU  - Fernandes, Ângela
AU  - Calhelha, Ricardo C.
AU  - Gavrilaş, Laura
AU  - Ferreira, Isabel C. F. R.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Babotă, Mihai
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.881210/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9168797
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5006
AB  - The members of Cystus genus are perenial shrubs with a well-established use in traditional medicine. Among these, C. creticus is the most popular, herbal preparations obtained from its aerial parts being recognized as antimicrobial, antitumor and anti-inflammatory agents. The present study aimed to evaluate phytochemical profile and bioactive potential of aqueous and hydroethanolic extracts of C. creticus aerial parts harvested from two different areas of Rhodes island. LC-DAD-ESI/MSn analysis revealed the presence of myricetin and quercetin glycosides as main compounds, especially in aqueous extracts, being probably responsible for their enhanced antioxidant and antimicrobial potential. On the other side, hydroethanolic preparations exerted a strong anti-inflammatory and anti-biofilm activity. Our findings suggest that the use of solvents with intermediate polarity can assure the best recovery of bioactive compounds from C. creticus, increasing the extraction yield for other non-phenolic compounds which can enhance therapeutic potential of the extract through a synergistic action.
T2  - Frontiers in Nutrition
T1  - Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.
VL  - 9
DO  - 10.3389/fnut.2022.881210
SP  - 881210
ER  - 

@article{
author = "Mocan, Andrei and Fernandes, Ângela and Calhelha, Ricardo C. and Gavrilaş, Laura and Ferreira, Isabel C. F. R. and Ivanov, Marija and Soković, Marina and Barros, Lillian and Babotă, Mihai",
year = "2022",
abstract = "The members of Cystus genus are perenial shrubs with a well-established use in traditional medicine. Among these, C. creticus is the most popular, herbal preparations obtained from its aerial parts being recognized as antimicrobial, antitumor and anti-inflammatory agents. The present study aimed to evaluate phytochemical profile and bioactive potential of aqueous and hydroethanolic extracts of C. creticus aerial parts harvested from two different areas of Rhodes island. LC-DAD-ESI/MSn analysis revealed the presence of myricetin and quercetin glycosides as main compounds, especially in aqueous extracts, being probably responsible for their enhanced antioxidant and antimicrobial potential. On the other side, hydroethanolic preparations exerted a strong anti-inflammatory and anti-biofilm activity. Our findings suggest that the use of solvents with intermediate polarity can assure the best recovery of bioactive compounds from C. creticus, increasing the extraction yield for other non-phenolic compounds which can enhance therapeutic potential of the extract through a synergistic action.",
journal = "Frontiers in Nutrition",
title = "Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.",
volume = "9",
doi = "10.3389/fnut.2022.881210",
pages = "881210"
}

Mocan, A., Fernandes, Â., Calhelha, R. C., Gavrilaş, L., Ferreira, I. C. F. R., Ivanov, M., Soković, M., Barros, L.,& Babotă, M.. (2022). Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.. in Frontiers in Nutrition, 9, 881210.
https://doi.org/10.3389/fnut.2022.881210

Mocan A, Fernandes Â, Calhelha RC, Gavrilaş L, Ferreira ICFR, Ivanov M, Soković M, Barros L, Babotă M. Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.. in Frontiers in Nutrition. 2022;9:881210.
doi:10.3389/fnut.2022.881210 .

Mocan, Andrei, Fernandes, Ângela, Calhelha, Ricardo C., Gavrilaş, Laura, Ferreira, Isabel C. F. R., Ivanov, Marija, Soković, Marina, Barros, Lillian, Babotă, Mihai, "Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island." in Frontiers in Nutrition, 9 (2022):881210,
https://doi.org/10.3389/fnut.2022.881210 . .