TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana
AU  - Milinčić, Danijel
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6687
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
VL  - 17
DO  - 10.3390/ph17040518
SP  - 518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana and Milinčić, Danijel and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
volume = "17",
doi = "10.3390/ph17040518",
pages = "518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M., Milinčić, D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
Basel: MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić M, Milinčić D, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana, Milinčić, Danijel, Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Milutinović, Violeta
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6453
AB  - Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)
IS  - 1
VL  - 13
DO  - 10.3390/antibiotics13010041
SP  - 41
ER  - 

@article{
author = "Ušjak, Ljuboš and Stojković, Dejan and Carević, Tamara and Milutinović, Violeta and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)",
number = "1",
volume = "13",
doi = "10.3390/antibiotics13010041",
pages = "41"
}

Ušjak, L., Stojković, D., Carević, T., Milutinović, V., Soković, M., Niketić, M.,& Petrović, S.. (2024). Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics
Basel: MDPI., 13(1), 41.
https://doi.org/10.3390/antibiotics13010041

Ušjak L, Stojković D, Carević T, Milutinović V, Soković M, Niketić M, Petrović S. Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics. 2024;13(1):41.
doi:10.3390/antibiotics13010041 .

Ušjak, Ljuboš, Stojković, Dejan, Carević, Tamara, Milutinović, Violeta, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)" in Antibiotics, 13, no. 1 (2024):41,
https://doi.org/10.3390/antibiotics13010041 . .

TY  - JOUR
AU  - Batinić, Petar
AU  - Čutović, Natalija
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Carević, Tamara
AU  - Prijić, Željana
AU  - Marinković, Aleksandar
AU  - Marković, Tatjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6680
AB  - This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kučaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy.
PB  - Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"
T2  - Lekovite sirovine
T1  - Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia
IS  - 1
VL  - 43
DO  - 10.61652/leksir2343e159B
SP  - e159
ER  - 

@article{
author = "Batinić, Petar and Čutović, Natalija and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Carević, Tamara and Prijić, Željana and Marinković, Aleksandar and Marković, Tatjana",
year = "2023",
abstract = "This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kučaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy.",
publisher = "Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"",
journal = "Lekovite sirovine",
title = "Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia",
number = "1",
volume = "43",
doi = "10.61652/leksir2343e159B",
pages = "e159"
}

Batinić, P., Čutović, N., Jovanović, A., Stojković, D., Zengin, G., Carević, T., Prijić, Ž., Marinković, A.,& Marković, T.. (2023). Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia. in Lekovite sirovine
Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"., 43(1), e159.
https://doi.org/10.61652/leksir2343e159B

Batinić P, Čutović N, Jovanović A, Stojković D, Zengin G, Carević T, Prijić Ž, Marinković A, Marković T. Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia. in Lekovite sirovine. 2023;43(1):e159.
doi:10.61652/leksir2343e159B .

Batinić, Petar, Čutović, Natalija, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Carević, Tamara, Prijić, Željana, Marinković, Aleksandar, Marković, Tatjana, "Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia" in Lekovite sirovine, 43, no. 1 (2023):e159,
https://doi.org/10.61652/leksir2343e159B . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5818
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
DO  - 10.3389/fevo.2023.1148309
SP  - 1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
doi = "10.3389/fevo.2023.1148309",
pages = "1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Babotă, Mihai
AU  - Frumuzachi, Oleg
AU  - Nicolescu, Alexandru
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Rocchetti, Gabriele
AU  - Zhang, Leilei
AU  - Lucini, Luigi
AU  - Crișan, Gianina
AU  - Mocan, Andrei
AU  - Vostinaru, Oliviu
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5502
AB  - Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in vivo diuretic effect and in vitro antimicrobial properties of three herbal preparations (infusion—TCI, tincture—TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method—OpTC) obtained from the aerial parts of T. comosus Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. In vivo diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. In vitro antibacterial and antifungal activities were assessed using p-iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63–7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, E. coli (MIC—0.38 mg/ml), B. cereus (MIC—0.75 mg/ml)), Penicillium funiculosum and P. verrucosum var. cyclopium (MIC—0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of T. comosus herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme T. comosus, this study being the first one that assessed the aforementioned bioactivities for this species.
PB  - Frontiers Media S.A.
T2  - Frontiers in Pharmacology
T1  - Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania
VL  - 14
DO  - 10.3389/fphar.2023.1115117
SP  - 1115117
ER  - 

@article{
author = "Babotă, Mihai and Frumuzachi, Oleg and Nicolescu, Alexandru and Stojković, Dejan and Soković, Marina and Rocchetti, Gabriele and Zhang, Leilei and Lucini, Luigi and Crișan, Gianina and Mocan, Andrei and Vostinaru, Oliviu",
year = "2023",
abstract = "Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in vivo diuretic effect and in vitro antimicrobial properties of three herbal preparations (infusion—TCI, tincture—TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method—OpTC) obtained from the aerial parts of T. comosus Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. In vivo diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. In vitro antibacterial and antifungal activities were assessed using p-iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63–7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, E. coli (MIC—0.38 mg/ml), B. cereus (MIC—0.75 mg/ml)), Penicillium funiculosum and P. verrucosum var. cyclopium (MIC—0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of T. comosus herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme T. comosus, this study being the first one that assessed the aforementioned bioactivities for this species.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Pharmacology",
title = "Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania",
volume = "14",
doi = "10.3389/fphar.2023.1115117",
pages = "1115117"
}

Babotă, M., Frumuzachi, O., Nicolescu, A., Stojković, D., Soković, M., Rocchetti, G., Zhang, L., Lucini, L., Crișan, G., Mocan, A.,& Vostinaru, O.. (2023). Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania. in Frontiers in Pharmacology
Frontiers Media S.A.., 14, 1115117.
https://doi.org/10.3389/fphar.2023.1115117

Babotă M, Frumuzachi O, Nicolescu A, Stojković D, Soković M, Rocchetti G, Zhang L, Lucini L, Crișan G, Mocan A, Vostinaru O. Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania. in Frontiers in Pharmacology. 2023;14:1115117.
doi:10.3389/fphar.2023.1115117 .

Babotă, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Stojković, Dejan, Soković, Marina, Rocchetti, Gabriele, Zhang, Leilei, Lucini, Luigi, Crișan, Gianina, Mocan, Andrei, Vostinaru, Oliviu, "Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania" in Frontiers in Pharmacology, 14 (2023):1115117,
https://doi.org/10.3389/fphar.2023.1115117 . .

TY  - JOUR
AU  - Joseph, Blessy
AU  - John, Amrit Jose
AU  - Glamočlija, Jasmina
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Lazović, Saša
AU  - Kochupurackal, Jayachandran
AU  - Kalarikkal, Nandakumar
AU  - Thomas, Sabu
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2352492822018025
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5348
AB  - Electrospun fibers with large surface area are of particular importance in the tissue engineering field as their highly porous structure favours cell adhesion, proliferation, and migration. Herein, we report a facile approach for the development of polycaprolactone (PCL) scaffolds incorporated with zirconium dioxide (ZrO2) nanoparticles. Zirconium dioxide nanoparticles were incorporated into the fibers at five different concentrations (0.5, 1, 3, 5 and 7 wt%), based on the amount of polymer. The morphology of the scaffolds was evaluated by scanning electron microscopy which showed interconnected pore structure. The incorporation of nanoparticles was confirmed from EDX studies. The functional groups were analysed using FTIR and crystallinity was analysed by XRD. Mechanical studies demonstrated that the scaffolds containing 1 wt% Zirconium dioxide nanoparticles exhibited the highest tensile strength. The scaffolds showed antibacterial activity against Gram positive bacterium Staphylococcus lugdunensis and were not active against Gram-negative bacterium Proteus vulgaris, at all time-points. ZrO2 loaded PCL scaffolds with good mechanical and biological properties will open avenues for the potential applications of the electrospun membranes for tissue engineering.
PB  - Elsevier Ltd
T2  - Materials Today Communications
T1  - Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds
VL  - 34
DO  - 10.1016/j.mtcomm.2022.104961
SP  - 104961
ER  - 

@article{
author = "Joseph, Blessy and John, Amrit Jose and Glamočlija, Jasmina and Stojković, Dejan and Soković, Marina and Lazović, Saša and Kochupurackal, Jayachandran and Kalarikkal, Nandakumar and Thomas, Sabu",
year = "2023",
abstract = "Electrospun fibers with large surface area are of particular importance in the tissue engineering field as their highly porous structure favours cell adhesion, proliferation, and migration. Herein, we report a facile approach for the development of polycaprolactone (PCL) scaffolds incorporated with zirconium dioxide (ZrO2) nanoparticles. Zirconium dioxide nanoparticles were incorporated into the fibers at five different concentrations (0.5, 1, 3, 5 and 7 wt%), based on the amount of polymer. The morphology of the scaffolds was evaluated by scanning electron microscopy which showed interconnected pore structure. The incorporation of nanoparticles was confirmed from EDX studies. The functional groups were analysed using FTIR and crystallinity was analysed by XRD. Mechanical studies demonstrated that the scaffolds containing 1 wt% Zirconium dioxide nanoparticles exhibited the highest tensile strength. The scaffolds showed antibacterial activity against Gram positive bacterium Staphylococcus lugdunensis and were not active against Gram-negative bacterium Proteus vulgaris, at all time-points. ZrO2 loaded PCL scaffolds with good mechanical and biological properties will open avenues for the potential applications of the electrospun membranes for tissue engineering.",
publisher = "Elsevier Ltd",
journal = "Materials Today Communications",
title = "Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds",
volume = "34",
doi = "10.1016/j.mtcomm.2022.104961",
pages = "104961"
}

Joseph, B., John, A. J., Glamočlija, J., Stojković, D., Soković, M., Lazović, S., Kochupurackal, J., Kalarikkal, N.,& Thomas, S.. (2023). Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds. in Materials Today Communications
Elsevier Ltd., 34, 104961.
https://doi.org/10.1016/j.mtcomm.2022.104961

Joseph B, John AJ, Glamočlija J, Stojković D, Soković M, Lazović S, Kochupurackal J, Kalarikkal N, Thomas S. Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds. in Materials Today Communications. 2023;34:104961.
doi:10.1016/j.mtcomm.2022.104961 .

Joseph, Blessy, John, Amrit Jose, Glamočlija, Jasmina, Stojković, Dejan, Soković, Marina, Lazović, Saša, Kochupurackal, Jayachandran, Kalarikkal, Nandakumar, Thomas, Sabu, "Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds" in Materials Today Communications, 34 (2023):104961,
https://doi.org/10.1016/j.mtcomm.2022.104961 . .

TY  - CONF
AU  - Rajaković, Mladen
AU  - Gašić, Uroš
AU  - Bukvički, Danka
AU  - Pešić, Mirjana
AU  - Ćirić, Ana
AU  - Milinčić, Danijel
AU  - Stojković, Dejan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6437
AB  - According to recent research, a variety of inedible portions of fruits and vegetables, as well as food waste, are excellent sources of phytochemicals that may be isolated and reintroduced as natural food additives into the food chain [1]. Besides the food industry, natural product from non-edible parts of the fruits of Cucurbitaceae family can be used in other industries such as cosmetic and pharmaceutical and the fact that the plants in this family are also used medicinally makes them excellent research subjects for the study of plants with high therapeutic potential [2]. The focus of our study was the solid/liquid extraction of lyophilized fruit peels of Cucumis metuliferus E. Mey. (Cucurbitaceae) under different extraction conditions such as extraction time (min), ethanol/water ratio (%) and power of ultrasonic bath (%). It was aimed as well to determine the phytochemical composition of 25 different ethanolic and hydro-ethanolic extracts by performing UHPLC-QToF-MS analysis. The Figure 1 shows the fruit of Horned Melon (C. metuliferus) and the exocarp as part of fruit used for extraction. Under different extraction conditions percentage yields of the dry extracts varied between 26.37% - 43.98%, obtaining the optimal extraction conditions as follows: EtOH (%): 50; Amplitude (%): 40; Time (min): 30. Furthermore, chemical profiling of the extracts revealed the presence of the following compounds: p-hydroxybenzoic acid, dihydroxybenzoic acid, galloyl pentoside, dihydroxybenzoyl pentoside, hydroxybenzoyl hexoside, vanilloyl hexoside and hydroxybenzoyl rhamnosyl hexoside. This is the first study that highlights the optimization of extraction conditions of the C. metuliferus peels, as well as chemical identification of bioactive compounds present in the extracts. Our future observations will be oriented towards exploring bioactive properties of the obtained extracts and further incorporations in food matrices.
PB  - Belgrade: Serbian Chemical Society
C3  - Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia
T1  - Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels
SP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6437
ER  - 

@conference{
author = "Rajaković, Mladen and Gašić, Uroš and Bukvički, Danka and Pešić, Mirjana and Ćirić, Ana and Milinčić, Danijel and Stojković, Dejan",
year = "2023",
abstract = "According to recent research, a variety of inedible portions of fruits and vegetables, as well as food waste, are excellent sources of phytochemicals that may be isolated and reintroduced as natural food additives into the food chain [1]. Besides the food industry, natural product from non-edible parts of the fruits of Cucurbitaceae family can be used in other industries such as cosmetic and pharmaceutical and the fact that the plants in this family are also used medicinally makes them excellent research subjects for the study of plants with high therapeutic potential [2]. The focus of our study was the solid/liquid extraction of lyophilized fruit peels of Cucumis metuliferus E. Mey. (Cucurbitaceae) under different extraction conditions such as extraction time (min), ethanol/water ratio (%) and power of ultrasonic bath (%). It was aimed as well to determine the phytochemical composition of 25 different ethanolic and hydro-ethanolic extracts by performing UHPLC-QToF-MS analysis. The Figure 1 shows the fruit of Horned Melon (C. metuliferus) and the exocarp as part of fruit used for extraction. Under different extraction conditions percentage yields of the dry extracts varied between 26.37% - 43.98%, obtaining the optimal extraction conditions as follows: EtOH (%): 50; Amplitude (%): 40; Time (min): 30. Furthermore, chemical profiling of the extracts revealed the presence of the following compounds: p-hydroxybenzoic acid, dihydroxybenzoic acid, galloyl pentoside, dihydroxybenzoyl pentoside, hydroxybenzoyl hexoside, vanilloyl hexoside and hydroxybenzoyl rhamnosyl hexoside. This is the first study that highlights the optimization of extraction conditions of the C. metuliferus peels, as well as chemical identification of bioactive compounds present in the extracts. Our future observations will be oriented towards exploring bioactive properties of the obtained extracts and further incorporations in food matrices.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia",
title = "Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels",
pages = "171",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6437"
}

Rajaković, M., Gašić, U., Bukvički, D., Pešić, M., Ćirić, A., Milinčić, D.,& Stojković, D.. (2023). Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels. in Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia
Belgrade: Serbian Chemical Society., 171.
https://hdl.handle.net/21.15107/rcub_ibiss_6437

Rajaković M, Gašić U, Bukvički D, Pešić M, Ćirić A, Milinčić D, Stojković D. Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels. in Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia. 2023;:171.
https://hdl.handle.net/21.15107/rcub_ibiss_6437 .

Rajaković, Mladen, Gašić, Uroš, Bukvički, Danka, Pešić, Mirjana, Ćirić, Ana, Milinčić, Danijel, Stojković, Dejan, "Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels" in Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia (2023):171,
https://hdl.handle.net/21.15107/rcub_ibiss_6437 .

TY  - CONF
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Petrović, Jovana
AU  - Stojković, Dejan
AU  - Gašić, Uroš
AU  - Milinčić, Danijel
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6463
AB  - Introduction: The food industry increasingly utilizes plant extracts over synthetic antioxidants for preventing lipid oxidation, fighting foodborne pathogens, and prolonging shelf life of various products. The bioactive potential varies among plant species. Traditionally, extracts from rosehip fruits (Rosa canina L.) have preventive and curative effects on diabetes, memory disorders and cancer. In addition, these extracts are often used as antimicrobial, antinociceptive and anti-inflammatory agents.
Objective: The aim of this work was to investigate phytochemical composition and biological potential of aqueous extracts from the fruits of R. canina. 
Methodology: The lyophilized aqueous extract was analyzed using Agilent 1290 Infinity ultra-high performance liquid chromatography (UHPLC) system in conjunction with quadrupole time-of-flight mass spectrometry. The extracts were analyzed for total polyphenol and flavonoid content (TPC and TFC, respectively) using spectrophotometric methods. The biological activities of aqueous extracts were evaluated under in vitro conditions. The Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assay were used to investigate antioxidant potential. Antimicrobial activity was determined using microdilution assay and crystal violet and MTT tests were used for evaluation of antibiofilm activity.
Results and conclusions: R. canina fruit's aqueous extract had 32 phenolic compounds, which were classified into eight classes. Qualitative phytochemical analyses showed that this extract contained flavan-3-ols and procyanidins, dominated by epicatechin (624.354 mg/100g) and a proanthocyanidin dimer hexoside type B (457.016 mg/100g). Furthermore, extract was high in total phenolic content (412.25 TE mg/g) but low in total flavonoid compounds (0.73 QE mg/g). The highest antioxidant capacity was measured by FRAP assay (103.29 AAE mg/g), whereas notable antimicrobial properties were determined as well, with a standout 67.68% antibiofilm activity against Candida spp. at 0.5 MIC. These findings highlight potential of R. canina as a dietary source of antioxidants and antimicrobial agents.
PB  - Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia
T1  - Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6463
ER  - 

@conference{
author = "Ćirić, Ana and Ivanov, Marija and Petrović, Jovana and Stojković, Dejan and Gašić, Uroš and Milinčić, Danijel and Pešić, Mirjana",
year = "2023",
abstract = "Introduction: The food industry increasingly utilizes plant extracts over synthetic antioxidants for preventing lipid oxidation, fighting foodborne pathogens, and prolonging shelf life of various products. The bioactive potential varies among plant species. Traditionally, extracts from rosehip fruits (Rosa canina L.) have preventive and curative effects on diabetes, memory disorders and cancer. In addition, these extracts are often used as antimicrobial, antinociceptive and anti-inflammatory agents.
Objective: The aim of this work was to investigate phytochemical composition and biological potential of aqueous extracts from the fruits of R. canina. 
Methodology: The lyophilized aqueous extract was analyzed using Agilent 1290 Infinity ultra-high performance liquid chromatography (UHPLC) system in conjunction with quadrupole time-of-flight mass spectrometry. The extracts were analyzed for total polyphenol and flavonoid content (TPC and TFC, respectively) using spectrophotometric methods. The biological activities of aqueous extracts were evaluated under in vitro conditions. The Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assay were used to investigate antioxidant potential. Antimicrobial activity was determined using microdilution assay and crystal violet and MTT tests were used for evaluation of antibiofilm activity.
Results and conclusions: R. canina fruit's aqueous extract had 32 phenolic compounds, which were classified into eight classes. Qualitative phytochemical analyses showed that this extract contained flavan-3-ols and procyanidins, dominated by epicatechin (624.354 mg/100g) and a proanthocyanidin dimer hexoside type B (457.016 mg/100g). Furthermore, extract was high in total phenolic content (412.25 TE mg/g) but low in total flavonoid compounds (0.73 QE mg/g). The highest antioxidant capacity was measured by FRAP assay (103.29 AAE mg/g), whereas notable antimicrobial properties were determined as well, with a standout 67.68% antibiofilm activity against Candida spp. at 0.5 MIC. These findings highlight potential of R. canina as a dietary source of antioxidants and antimicrobial agents.",
publisher = "Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia",
title = "Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6463"
}

Ćirić, A., Ivanov, M., Petrović, J., Stojković, D., Gašić, U., Milinčić, D.,& Pešić, M.. (2023). Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications. in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia
Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_6463

Ćirić A, Ivanov M, Petrović J, Stojković D, Gašić U, Milinčić D, Pešić M. Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications. in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia. 2023;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_6463 .

Ćirić, Ana, Ivanov, Marija, Petrović, Jovana, Stojković, Dejan, Gašić, Uroš, Milinčić, Danijel, Pešić, Mirjana, "Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications" in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia (2023):41,
https://hdl.handle.net/21.15107/rcub_ibiss_6463 .

TY  - CONF
AU  - Stojković, Dejan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5559
AB  - In the food sector, 30% of the food that is produced annually is wasted globally. 1300 million tons of food,
$1 billion in economic costs, $700 billion in environmental costs, and almost $900 billion in social costs total
this. In addition, the fruits and vegetables' peels, leaves, roots, tubers, and seeds that are thrown away
every year contribute to between 25% and 30% of the waste produced by the food sector. In Europe, one
of the industries that produce a significant volume of bio-waste is the food industry. Food waste in Europe
totals over 1.3 billion tons a year, of which 700 million tons come from agriculture. These waste materials,
which largely come from the processing of cereals, fruits, and vegetables, have a significant potential for
recycling.
Using increased conversion techniques, these leftovers can be converted into resources that could lead to
the production of bio-products that are theoretically sustainable, including energy, fertilizers, materials, and
molecules. In this regard, the valorization of residues and agri-food by-products is currently presented not
only as a requirement, but also as a chance to gain new products of high value and of great impact on the
economy of the industrial sector.
The use of kiwano peel extracts to obtain bioactive ingredients, eggplant fruit bio-residues, kiwi peels and
pineapple peels and crown leaves is studied and presented. Findings reveal that all of these bio residual
extracts from industrial bio-waste are good source of phenolic compounds with bioactive properties that
could be effectively incorporated into food matrices.
PB  - Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences
C3  - Program and Book of Abstracts: Sustainable Utilization of Bio-Resources and Waste of Medicinal and Aromatic Plants for Innovative Bioactive Products - ICSUMAP’23; 2023 Mar 27-28; Sofia, Bulgaria
T1  - Plant industrial by-products as sources of bioactive compounds with potential food and cosmeceutical applications
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5559
ER  - 

@conference{
author = "Stojković, Dejan",
year = "2023",
abstract = "In the food sector, 30% of the food that is produced annually is wasted globally. 1300 million tons of food,
$1 billion in economic costs, $700 billion in environmental costs, and almost $900 billion in social costs total
this. In addition, the fruits and vegetables' peels, leaves, roots, tubers, and seeds that are thrown away
every year contribute to between 25% and 30% of the waste produced by the food sector. In Europe, one
of the industries that produce a significant volume of bio-waste is the food industry. Food waste in Europe
totals over 1.3 billion tons a year, of which 700 million tons come from agriculture. These waste materials,
which largely come from the processing of cereals, fruits, and vegetables, have a significant potential for
recycling.
Using increased conversion techniques, these leftovers can be converted into resources that could lead to
the production of bio-products that are theoretically sustainable, including energy, fertilizers, materials, and
molecules. In this regard, the valorization of residues and agri-food by-products is currently presented not
only as a requirement, but also as a chance to gain new products of high value and of great impact on the
economy of the industrial sector.
The use of kiwano peel extracts to obtain bioactive ingredients, eggplant fruit bio-residues, kiwi peels and
pineapple peels and crown leaves is studied and presented. Findings reveal that all of these bio residual
extracts from industrial bio-waste are good source of phenolic compounds with bioactive properties that
could be effectively incorporated into food matrices.",
publisher = "Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences",
journal = "Program and Book of Abstracts: Sustainable Utilization of Bio-Resources and Waste of Medicinal and Aromatic Plants for Innovative Bioactive Products - ICSUMAP’23; 2023 Mar 27-28; Sofia, Bulgaria",
title = "Plant industrial by-products as sources of bioactive compounds with potential food and cosmeceutical applications",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5559"
}

Stojković, D.. (2023). Plant industrial by-products as sources of bioactive compounds with potential food and cosmeceutical applications. in Program and Book of Abstracts: Sustainable Utilization of Bio-Resources and Waste of Medicinal and Aromatic Plants for Innovative Bioactive Products - ICSUMAP’23; 2023 Mar 27-28; Sofia, Bulgaria
Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences., 17.
https://hdl.handle.net/21.15107/rcub_ibiss_5559

Stojković D. Plant industrial by-products as sources of bioactive compounds with potential food and cosmeceutical applications. in Program and Book of Abstracts: Sustainable Utilization of Bio-Resources and Waste of Medicinal and Aromatic Plants for Innovative Bioactive Products - ICSUMAP’23; 2023 Mar 27-28; Sofia, Bulgaria. 2023;:17.
https://hdl.handle.net/21.15107/rcub_ibiss_5559 .

Stojković, Dejan, "Plant industrial by-products as sources of bioactive compounds with potential food and cosmeceutical applications" in Program and Book of Abstracts: Sustainable Utilization of Bio-Resources and Waste of Medicinal and Aromatic Plants for Innovative Bioactive Products - ICSUMAP’23; 2023 Mar 27-28; Sofia, Bulgaria (2023):17,
https://hdl.handle.net/21.15107/rcub_ibiss_5559 .

TY  - JOUR
AU  - Carević, Tamara
AU  - Stojković, Dejan
AU  - Ivanov, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6073
AB  - Flavonoids are secondary metabolites abundantly present in plants and, in most cases, essential contributors to plants bioactivity. They have been studied so far for a range of possible health-beneficial effects, including antioxidant, cardioprotective, and cytotoxic. Therefore, there are data on the antimicrobial potential of a significant number of flavonoids. However, less is known regarding their antivirulence traits. Trending antimicrobial research worldwide has pointed out the promising effects of antimicrobial strategies based on the antivirulence principle, so this review aims to present the newest research regarding the antivirulence effects of flavonoids. Articles on antivirulence flavonoids published from 2015 until now were selected. A range of molecules from this class has been studied up to date, with the most abundant data for quercetin and myricetin, while the most studied organism is Pseudomonas aeruginosa</i>. The antivirulence attributes studied included antibiofilm assessment, followed by data on the inhibition of virulence pigments (pyocyanin, violacein, and staphyloxanthin) and virulence enzyme production (such as sortase A and elastase). Less information is collected on the inhibition of morphological transition, motility, and molecular mechanisms underlying the antivirulence properties of flavonoids and in vivo</i> research. Flavonoids are a group of compounds with a wide range of antivirulence traits and might be further developed into essential parts of novel antimicrobial strategies.
PB  - Bentham Science Publication Ltd.
T2  - Current Pharmaceutical Design
T1  - Plant Flavonoids as Reservoirs of Therapeutics against Microbial Virulence Traits: A Comprehensive Review Update
IS  - 12
VL  - 29
DO  - 10.2174/1381612829666230413085029
SP  - 914
EP  - 927
ER  - 

@article{
author = "Carević, Tamara and Stojković, Dejan and Ivanov, Marija",
year = "2023",
abstract = "Flavonoids are secondary metabolites abundantly present in plants and, in most cases, essential contributors to plants bioactivity. They have been studied so far for a range of possible health-beneficial effects, including antioxidant, cardioprotective, and cytotoxic. Therefore, there are data on the antimicrobial potential of a significant number of flavonoids. However, less is known regarding their antivirulence traits. Trending antimicrobial research worldwide has pointed out the promising effects of antimicrobial strategies based on the antivirulence principle, so this review aims to present the newest research regarding the antivirulence effects of flavonoids. Articles on antivirulence flavonoids published from 2015 until now were selected. A range of molecules from this class has been studied up to date, with the most abundant data for quercetin and myricetin, while the most studied organism is Pseudomonas aeruginosa</i>. The antivirulence attributes studied included antibiofilm assessment, followed by data on the inhibition of virulence pigments (pyocyanin, violacein, and staphyloxanthin) and virulence enzyme production (such as sortase A and elastase). Less information is collected on the inhibition of morphological transition, motility, and molecular mechanisms underlying the antivirulence properties of flavonoids and in vivo</i> research. Flavonoids are a group of compounds with a wide range of antivirulence traits and might be further developed into essential parts of novel antimicrobial strategies.",
publisher = "Bentham Science Publication Ltd.",
journal = "Current Pharmaceutical Design",
title = "Plant Flavonoids as Reservoirs of Therapeutics against Microbial Virulence Traits: A Comprehensive Review Update",
number = "12",
volume = "29",
doi = "10.2174/1381612829666230413085029",
pages = "914-927"
}

Carević, T., Stojković, D.,& Ivanov, M.. (2023). Plant Flavonoids as Reservoirs of Therapeutics against Microbial Virulence Traits: A Comprehensive Review Update. in Current Pharmaceutical Design
Bentham Science Publication Ltd.., 29(12), 914-927.
https://doi.org/10.2174/1381612829666230413085029

Carević T, Stojković D, Ivanov M. Plant Flavonoids as Reservoirs of Therapeutics against Microbial Virulence Traits: A Comprehensive Review Update. in Current Pharmaceutical Design. 2023;29(12):914-927.
doi:10.2174/1381612829666230413085029 .

Carević, Tamara, Stojković, Dejan, Ivanov, Marija, "Plant Flavonoids as Reservoirs of Therapeutics against Microbial Virulence Traits: A Comprehensive Review Update" in Current Pharmaceutical Design, 29, no. 12 (2023):914-927,
https://doi.org/10.2174/1381612829666230413085029 . .

TY  - JOUR
AU  - Marković, Tatjana
AU  - Čutović, Natalija
AU  - Carević, Tamara
AU  - Gašić, Uroš
AU  - Stojković, Dejan
AU  - Xue, Jingqi
AU  - Jovanović, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6072
AB  - Paeonia peregrina Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of P. peregrina petals was Pančevo (South Banat).
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts
IS  - 14
VL  - 24
DO  - 10.3390/ijms241411764
SP  - 11764
ER  - 

@article{
author = "Marković, Tatjana and Čutović, Natalija and Carević, Tamara and Gašić, Uroš and Stojković, Dejan and Xue, Jingqi and Jovanović, Aleksandra",
year = "2023",
abstract = "Paeonia peregrina Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of P. peregrina petals was Pančevo (South Banat).",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts",
number = "14",
volume = "24",
doi = "10.3390/ijms241411764",
pages = "11764"
}

Marković, T., Čutović, N., Carević, T., Gašić, U., Stojković, D., Xue, J.,& Jovanović, A.. (2023). Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts. in International Journal of Molecular Sciences
Basel: MDPI., 24(14), 11764.
https://doi.org/10.3390/ijms241411764

Marković T, Čutović N, Carević T, Gašić U, Stojković D, Xue J, Jovanović A. Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts. in International Journal of Molecular Sciences. 2023;24(14):11764.
doi:10.3390/ijms241411764 .

Marković, Tatjana, Čutović, Natalija, Carević, Tamara, Gašić, Uroš, Stojković, Dejan, Xue, Jingqi, Jovanović, Aleksandra, "Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts" in International Journal of Molecular Sciences, 24, no. 14 (2023):11764,
https://doi.org/10.3390/ijms241411764 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Petrović, Jovana
AU  - Carević, Tamara
AU  - Soković, Marina
AU  - Liaras, Konstantinos
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5793
AB  - This narrative review paper provides an up-to-date overview of the potential of novel synthetic and semisynthetic compounds as antibacterials that target virulence traits in resistant strains. The review focused on research conducted in the last five years and investigated a range of compounds including azoles, indoles, thiophenes, glycopeptides, pleuromutilin derivatives, lactone derivatives, and chalcones. The emergence and spread of antibiotic-resistant bacterial strains is a growing public health concern, and new approaches are urgently needed to combat this threat. One promising approach is to target virulence factors, which are essential for bacterial survival and pathogenesis, but not for bacterial growth. By targeting virulence factors, it may be possible to reduce the severity of bacterial infections without promoting the development of resistance. We discuss the mechanisms of action of the various compounds investigated and their potential as antibacterials. The review highlights the potential of targeting virulence factors as a promising strategy to combat antibiotic resistance and suggests that further research is needed to identify new compounds and optimize their efficacy. The findings of this review suggest that novel synthetic and semisynthetic compounds that target virulence factors have great potential as antibacterials in the fight against antibiotic resistance.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review
IS  - 6
VL  - 12
DO  - 10.3390/antibiotics12060963
SP  - 963
ER  - 

@article{
author = "Stojković, Dejan and Petrović, Jovana and Carević, Tamara and Soković, Marina and Liaras, Konstantinos",
year = "2023",
abstract = "This narrative review paper provides an up-to-date overview of the potential of novel synthetic and semisynthetic compounds as antibacterials that target virulence traits in resistant strains. The review focused on research conducted in the last five years and investigated a range of compounds including azoles, indoles, thiophenes, glycopeptides, pleuromutilin derivatives, lactone derivatives, and chalcones. The emergence and spread of antibiotic-resistant bacterial strains is a growing public health concern, and new approaches are urgently needed to combat this threat. One promising approach is to target virulence factors, which are essential for bacterial survival and pathogenesis, but not for bacterial growth. By targeting virulence factors, it may be possible to reduce the severity of bacterial infections without promoting the development of resistance. We discuss the mechanisms of action of the various compounds investigated and their potential as antibacterials. The review highlights the potential of targeting virulence factors as a promising strategy to combat antibiotic resistance and suggests that further research is needed to identify new compounds and optimize their efficacy. The findings of this review suggest that novel synthetic and semisynthetic compounds that target virulence factors have great potential as antibacterials in the fight against antibiotic resistance.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review",
number = "6",
volume = "12",
doi = "10.3390/antibiotics12060963",
pages = "963"
}

Stojković, D., Petrović, J., Carević, T., Soković, M.,& Liaras, K.. (2023). Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review. in Antibiotics
Basel: MDPI., 12(6), 963.
https://doi.org/10.3390/antibiotics12060963

Stojković D, Petrović J, Carević T, Soković M, Liaras K. Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review. in Antibiotics. 2023;12(6):963.
doi:10.3390/antibiotics12060963 .

Stojković, Dejan, Petrović, Jovana, Carević, Tamara, Soković, Marina, Liaras, Konstantinos, "Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review" in Antibiotics, 12, no. 6 (2023):963,
https://doi.org/10.3390/antibiotics12060963 . .

TY  - CONF
AU  - Stojković, Dejan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5503
AB  - Mnoge aromatične i lekovite biljke sadrže veliki broj bioaktivnih komponenti, pre svega fenolnih jedinjenja, koja se, prema brojnim naučnim istraživanjima, dovode u vezu sa biološkom aktivnošću ovih biljaka. Anti-inflamatorna, antioksidativna i antibakterijska svojstva ovih jedinjenja izazvala su veliko interesovanje u prehrambenoj, farmaceutskoj i kozmetičkoj industriji, između ostalih. Proučavani su fenolni profili odabranih biljaka koje su korišćene u brojnim tradicionalnim preparatima: Allium scorodoprassum, Anthriscus cerefolium, Artemisia absinthium, Calluna vulgaris, Carpobrotus edulis, Cytisus multiflorus, Genista tridentata, Hypericum alpigenum, H. perforatum, H. rochelii, Juglans regia, Phlomis fruticosa, Salvia nemorosa, Seseli gummiferum, S. transcaucasicum, Verbascum sinuatum. Pored toga, in vitro eksperimenti su korišćeni za procenu antioksidativne, antibakterijske, antiinflamatorne i citotoksične efikasnosti odabranih biljnih ekstrakata. Antioksidativna aktivnost je ispitivana pomoću nekoliko različitih testova uključujući TBARS, OxHLIA, DPPH, ABTS, CUPRAC, FRAP, test heliranja metala i fosfomolibden. Fenolna jedinjenja, koja pripadaju fenolnim kiselinama i flavonoidima su identifikovana kao najdominantniji sastojci u ispitivanim biljnim vrstama. Štaviše, istraživani ekstrakti su pokazali značajnu antimikrobno dejstvo protiv bakterija i mikrogljiva iz hrane, patogena ljudi, životinja i biljaka. Ispitivani ekstrakti su posedovali antioksidativno dejstvo, antiinflamatorna svojstva što je potvrđeno putem nekoliko različitih testova. Nalazi ove studije poboljšavaju razumevanje fenolnih profila i takođe nude dokaz o bioaktivnim kvalitetama ispitivanih lekovitih vrsta, što ih čini potencijalno veoma atraktivnim kandidatima za proučavanje i upotrebu u različitim oblastima, uključujući hranu, kozmetiku i farmaceutske proizvode.
PB  - Belgrade: Faculty of Forestry, University of Belgrade
C3  - Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia
T1  - Od tradicije do zdravlja: hemijska karakterizacija i biološka aktivnost odabranih lekovitih biljaka
SP  - 183
EP  - 191
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5503
ER  - 

@conference{
author = "Stojković, Dejan",
year = "2023",
abstract = "Mnoge aromatične i lekovite biljke sadrže veliki broj bioaktivnih komponenti, pre svega fenolnih jedinjenja, koja se, prema brojnim naučnim istraživanjima, dovode u vezu sa biološkom aktivnošću ovih biljaka. Anti-inflamatorna, antioksidativna i antibakterijska svojstva ovih jedinjenja izazvala su veliko interesovanje u prehrambenoj, farmaceutskoj i kozmetičkoj industriji, između ostalih. Proučavani su fenolni profili odabranih biljaka koje su korišćene u brojnim tradicionalnim preparatima: Allium scorodoprassum, Anthriscus cerefolium, Artemisia absinthium, Calluna vulgaris, Carpobrotus edulis, Cytisus multiflorus, Genista tridentata, Hypericum alpigenum, H. perforatum, H. rochelii, Juglans regia, Phlomis fruticosa, Salvia nemorosa, Seseli gummiferum, S. transcaucasicum, Verbascum sinuatum. Pored toga, in vitro eksperimenti su korišćeni za procenu antioksidativne, antibakterijske, antiinflamatorne i citotoksične efikasnosti odabranih biljnih ekstrakata. Antioksidativna aktivnost je ispitivana pomoću nekoliko različitih testova uključujući TBARS, OxHLIA, DPPH, ABTS, CUPRAC, FRAP, test heliranja metala i fosfomolibden. Fenolna jedinjenja, koja pripadaju fenolnim kiselinama i flavonoidima su identifikovana kao najdominantniji sastojci u ispitivanim biljnim vrstama. Štaviše, istraživani ekstrakti su pokazali značajnu antimikrobno dejstvo protiv bakterija i mikrogljiva iz hrane, patogena ljudi, životinja i biljaka. Ispitivani ekstrakti su posedovali antioksidativno dejstvo, antiinflamatorna svojstva što je potvrđeno putem nekoliko različitih testova. Nalazi ove studije poboljšavaju razumevanje fenolnih profila i takođe nude dokaz o bioaktivnim kvalitetama ispitivanih lekovitih vrsta, što ih čini potencijalno veoma atraktivnim kandidatima za proučavanje i upotrebu u različitim oblastima, uključujući hranu, kozmetiku i farmaceutske proizvode.",
publisher = "Belgrade: Faculty of Forestry, University of Belgrade",
journal = "Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia",
title = "Od tradicije do zdravlja: hemijska karakterizacija i biološka aktivnost odabranih lekovitih biljaka",
pages = "183-191",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5503"
}

Stojković, D.. (2023). Od tradicije do zdravlja: hemijska karakterizacija i biološka aktivnost odabranih lekovitih biljaka. in Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia
Belgrade: Faculty of Forestry, University of Belgrade., 183-191.
https://hdl.handle.net/21.15107/rcub_ibiss_5503

Stojković D. Od tradicije do zdravlja: hemijska karakterizacija i biološka aktivnost odabranih lekovitih biljaka. in Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia. 2023;:183-191.
https://hdl.handle.net/21.15107/rcub_ibiss_5503 .

Stojković, Dejan, "Od tradicije do zdravlja: hemijska karakterizacija i biološka aktivnost odabranih lekovitih biljaka" in Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia (2023):183-191,
https://hdl.handle.net/21.15107/rcub_ibiss_5503 .

TY  - JOUR
AU  - Božunović, Jelena
AU  - Ivanov, Marija
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Nakarada, Đura
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Giba, Zlatko
AU  - Mišić, Danijela
AU  - Stojković, Dejan
PY  - 2023
UR  - https://www.mdpi.com/1424-8247/16/2/245
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5490
AB  - Spiked centaury (Centaurium spicatum) is a well-known medicinal plant from the Mediterranean region with various bioactivities, but there are no studies addressing the use of different solvent systems to improve its pharmacological potential. Nine extraction procedures were adapted to study the effects of solvent composition on the content of bioactive compounds in C. spicatum extracts and on corresponding bioactivities. Targeted metabolomics was performed to obtain information on the chemical composition of extracts. Ethanol-water-based extraction procedures were the most efficient in isolating polyphenols, while less polar butanol extract contained the highest amount of iridoids. Antioxidant potential analysis revealed stronger activity in extracts with higher polyphenol content. Bacillus cereus and Staphylococus aureus were designated as the most sensitive bacterial strains to the activity of extracts, while among the micromycetes tested, Penicillium funiculosum was the most susceptible strain. Butanol extract showed antivirulence potential on Candida albicans morphological transition from yeast to hyphal form, and selected extracts were effective against biofilm formation in two Candida species. All the extracts tested in this study showed no cytotoxic activity to immortalize human skin keratinocyte cell line (HaCaT), whereas extracts obtained by ethanol-water extraction stand out for their potent wound healing effects. Moreover, the influence of the extraction solvent system on various bioactivities of C. spicatum is reported herein for the first time. Overall, the results presented in this study promote the use of C. spicatum as a source of natural products with potential antioxidant, wound healing, and antimicrobial applications that are potentially safe for human use.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts
IS  - 2
VL  - 16
DO  - 10.3390/ph16020245
SP  - 245
ER  - 

@article{
author = "Božunović, Jelena and Ivanov, Marija and Petrović, Jovana and Gašić, Uroš and Nakarada, Đura and Milutinović, Milica and Aničić, Neda and Giba, Zlatko and Mišić, Danijela and Stojković, Dejan",
year = "2023",
abstract = "Spiked centaury (Centaurium spicatum) is a well-known medicinal plant from the Mediterranean region with various bioactivities, but there are no studies addressing the use of different solvent systems to improve its pharmacological potential. Nine extraction procedures were adapted to study the effects of solvent composition on the content of bioactive compounds in C. spicatum extracts and on corresponding bioactivities. Targeted metabolomics was performed to obtain information on the chemical composition of extracts. Ethanol-water-based extraction procedures were the most efficient in isolating polyphenols, while less polar butanol extract contained the highest amount of iridoids. Antioxidant potential analysis revealed stronger activity in extracts with higher polyphenol content. Bacillus cereus and Staphylococus aureus were designated as the most sensitive bacterial strains to the activity of extracts, while among the micromycetes tested, Penicillium funiculosum was the most susceptible strain. Butanol extract showed antivirulence potential on Candida albicans morphological transition from yeast to hyphal form, and selected extracts were effective against biofilm formation in two Candida species. All the extracts tested in this study showed no cytotoxic activity to immortalize human skin keratinocyte cell line (HaCaT), whereas extracts obtained by ethanol-water extraction stand out for their potent wound healing effects. Moreover, the influence of the extraction solvent system on various bioactivities of C. spicatum is reported herein for the first time. Overall, the results presented in this study promote the use of C. spicatum as a source of natural products with potential antioxidant, wound healing, and antimicrobial applications that are potentially safe for human use.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts",
number = "2",
volume = "16",
doi = "10.3390/ph16020245",
pages = "245"
}

Božunović, J., Ivanov, M., Petrović, J., Gašić, U., Nakarada, Đ., Milutinović, M., Aničić, N., Giba, Z., Mišić, D.,& Stojković, D.. (2023). Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts. in Pharmaceuticals
Basel: MDPI., 16(2), 245.
https://doi.org/10.3390/ph16020245

Božunović J, Ivanov M, Petrović J, Gašić U, Nakarada Đ, Milutinović M, Aničić N, Giba Z, Mišić D, Stojković D. Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts. in Pharmaceuticals. 2023;16(2):245.
doi:10.3390/ph16020245 .

Božunović, Jelena, Ivanov, Marija, Petrović, Jovana, Gašić, Uroš, Nakarada, Đura, Milutinović, Milica, Aničić, Neda, Giba, Zlatko, Mišić, Danijela, Stojković, Dejan, "Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts" in Pharmaceuticals, 16, no. 2 (2023):245,
https://doi.org/10.3390/ph16020245 . .

TY  - JOUR
AU  - Babota, Mihai
AU  - Frumuzachi, Oleg
AU  - Nicolescu, Alexandru
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Barros, Lillian
AU  - Añibarro-Ortega, Mikel
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Mocan, Andrei
AU  - López, Víctor
AU  - Crișan, Gianina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5475
AB  - Wild thyme aerial parts (Serpylli herba) are recognized as a valuable herbal product with antioxidant, anti-inflammatory, and antibacterial effects. Although pharmacopoeial regulations allow its collection exclusively from Thymus serpyllum, substitution with other species is frequent in current practice. This study analyzed the phenolic composition, antioxidant, and enzyme-inhibitory and antimicrobial activity of the hydroethanolic extracts obtained from five Romanian wild thyme species (Thymus alpestris, T. glabrescens, T. panonicus, T. pulcherimus and T. pulegioides). The analysis of individual phenolic constituents was performed through LC-ESI-DAD/MS2, while for the in vitro evaluation of antioxidant potential, TEAC, FRAP, DPPH, TBARS and OxHLIA assays were employed. The anti-enzymatic potential was tested in vitro against tyrosinase, α-glucosidase and acetylcholinesterase. High rosmarinic acid contents were quantified in all species (20.06 ± 0.32–80.49 ± 0.001 mg/g dry extract); phenolic acids derivatives (including salvianolic acids) were confirmed as the principal metabolites of T. alpestris and T. glabrescens, while eriodictyol-O-di-hexoside was found exclusively in T. alpestris. All species showed strong antioxidant potential and moderate anti-enzymatic effect against α-glucosidase and acetylcholinesterase, showing no anti-tyrosinase activity. This is the first detailed report on the chemical and biological profile of T. alpestris collected from Romanian spontaneous flora.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits
IS  - 2
VL  - 12
DO  - 10.3390/antiox12020390
SP  - 390
ER  - 

@article{
author = "Babota, Mihai and Frumuzachi, Oleg and Nicolescu, Alexandru and Dias, Maria Inês and Pinela, José and Barros, Lillian and Añibarro-Ortega, Mikel and Stojković, Dejan and Carević, Tamara and Mocan, Andrei and López, Víctor and Crișan, Gianina",
year = "2023",
abstract = "Wild thyme aerial parts (Serpylli herba) are recognized as a valuable herbal product with antioxidant, anti-inflammatory, and antibacterial effects. Although pharmacopoeial regulations allow its collection exclusively from Thymus serpyllum, substitution with other species is frequent in current practice. This study analyzed the phenolic composition, antioxidant, and enzyme-inhibitory and antimicrobial activity of the hydroethanolic extracts obtained from five Romanian wild thyme species (Thymus alpestris, T. glabrescens, T. panonicus, T. pulcherimus and T. pulegioides). The analysis of individual phenolic constituents was performed through LC-ESI-DAD/MS2, while for the in vitro evaluation of antioxidant potential, TEAC, FRAP, DPPH, TBARS and OxHLIA assays were employed. The anti-enzymatic potential was tested in vitro against tyrosinase, α-glucosidase and acetylcholinesterase. High rosmarinic acid contents were quantified in all species (20.06 ± 0.32–80.49 ± 0.001 mg/g dry extract); phenolic acids derivatives (including salvianolic acids) were confirmed as the principal metabolites of T. alpestris and T. glabrescens, while eriodictyol-O-di-hexoside was found exclusively in T. alpestris. All species showed strong antioxidant potential and moderate anti-enzymatic effect against α-glucosidase and acetylcholinesterase, showing no anti-tyrosinase activity. This is the first detailed report on the chemical and biological profile of T. alpestris collected from Romanian spontaneous flora.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits",
number = "2",
volume = "12",
doi = "10.3390/antiox12020390",
pages = "390"
}

Babota, M., Frumuzachi, O., Nicolescu, A., Dias, M. I., Pinela, J., Barros, L., Añibarro-Ortega, M., Stojković, D., Carević, T., Mocan, A., López, V.,& Crișan, G.. (2023). Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits. in Antioxidants
Basel: MDPI., 12(2), 390.
https://doi.org/10.3390/antiox12020390

Babota M, Frumuzachi O, Nicolescu A, Dias MI, Pinela J, Barros L, Añibarro-Ortega M, Stojković D, Carević T, Mocan A, López V, Crișan G. Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits. in Antioxidants. 2023;12(2):390.
doi:10.3390/antiox12020390 .

Babota, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Dias, Maria Inês, Pinela, José, Barros, Lillian, Añibarro-Ortega, Mikel, Stojković, Dejan, Carević, Tamara, Mocan, Andrei, López, Víctor, Crișan, Gianina, "Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits" in Antioxidants, 12, no. 2 (2023):390,
https://doi.org/10.3390/antiox12020390 . .

TY  - JOUR
AU  - Đorđevski, Nikoleta
AU  - Uba, Abdullahi Ibrahim
AU  - Zengin, Gokhan
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Ristanović, Elizabeta
AU  - Ćirić, Ana
AU  - Nikolić, Biljana
AU  - Stojković, Dejan
PY  - 2023
UR  - https://www.mdpi.com/1424-8247/16/1/21
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9865742
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5433
AB  - This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of Allium scorodoprasum L. (Amaryllidaceae)-the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(-)HESI-QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3-O-glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of A. scorodoprasum with contemporary in vitro scientific approaches.
PB  - Basel: MDPI
T2  - Pharmaceuticals (Basel, Switzerland)
T1  - Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts
IS  - 1
VL  - 16
DO  - 10.3390/ph16010021
SP  - 21
ER  - 

@article{
author = "Đorđevski, Nikoleta and Uba, Abdullahi Ibrahim and Zengin, Gokhan and Božunović, Jelena and Gašić, Uroš and Ristanović, Elizabeta and Ćirić, Ana and Nikolić, Biljana and Stojković, Dejan",
year = "2023",
abstract = "This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of Allium scorodoprasum L. (Amaryllidaceae)-the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(-)HESI-QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3-O-glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of A. scorodoprasum with contemporary in vitro scientific approaches.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals (Basel, Switzerland)",
title = "Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts",
number = "1",
volume = "16",
doi = "10.3390/ph16010021",
pages = "21"
}

Đorđevski, N., Uba, A. I., Zengin, G., Božunović, J., Gašić, U., Ristanović, E., Ćirić, A., Nikolić, B.,& Stojković, D.. (2023). Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts. in Pharmaceuticals (Basel, Switzerland)
Basel: MDPI., 16(1), 21.
https://doi.org/10.3390/ph16010021

Đorđevski N, Uba AI, Zengin G, Božunović J, Gašić U, Ristanović E, Ćirić A, Nikolić B, Stojković D. Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts. in Pharmaceuticals (Basel, Switzerland). 2023;16(1):21.
doi:10.3390/ph16010021 .

Đorđevski, Nikoleta, Uba, Abdullahi Ibrahim, Zengin, Gokhan, Božunović, Jelena, Gašić, Uroš, Ristanović, Elizabeta, Ćirić, Ana, Nikolić, Biljana, Stojković, Dejan, "Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts" in Pharmaceuticals (Basel, Switzerland), 16, no. 1 (2023):21,
https://doi.org/10.3390/ph16010021 . .

TY  - CONF
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Stojković, Dejan
AU  - Dmitrović, Slavica
AU  - Nestorović Živković, Jasmina
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Božunović, Jelena
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5320
AB  - Trichoderma spp. have been extensively explored and used in agriculture due to their well-known biological control mechanisms. Fungi of the genus Trichoderma produce secondary metabolites which affect plant metabolism by stimulating the production of defense-related compounds and increasing the antioxidant capacity through enhanced polyphenol content and elevated activity of antioxidant enzymes, a result of the excess production of reactive oxygen species. In this work, Nepeta sibirica plants were grown in vitro on solid medium treated with two Trichoderma strains (T. viride and T. harzianum). After three and six days of treatment, leaves were metabolically profiled for major phenolic acids (chlorogenic, caffeic, and rosmarinic acid), and iridoids content (cis,trans-nepetalactone and 1,5,9-epideoxyloganic acid), in parallel with the expression analysis of nepetalactone biosynthetic genes and regulatory genetic elements-transcription factors (TFs). Both fungal strains induced changes in phenolic acids production, while only T. harzianum induced elevated levels of iridoids. Biosynthetic genes GPPS, and IS, as well as TF MYC2, were the only genes with expression levels not affected by the treatments with micro fungi. The response of antioxidant enzymes in N. sibirica leaves was also studied. The most active antioxidant enzyme following the infection with T. viride and T. harzianum was peroxidase (POX). Catalase (CAT) and superoxide dismutase (SOD) activity were also affected by the treatments. Results indicate a possibility of using Trichoderma infection in N. sibirica to elicit the production of biologically active defense compounds.
PB  - Belgrade : Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Alterations in specialized metabolism and antioxidant capacity of Nepeta sibirica L. as induced by two Trichoderma sp.
SP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5320
ER  - 

@conference{
author = "Aničić, Neda and Matekalo, Dragana and Stojković, Dejan and Dmitrović, Slavica and Nestorović Živković, Jasmina and Skorić, Marijana and Gašić, Uroš and Milutinović, Milica and Petrović, Luka and Božunović, Jelena and Filipović, Biljana and Banjanac, Tijana and Šiler, Branislav and Todorović, Miloš and Lukić, Tamara and Mišić, Danijela",
year = "2022",
abstract = "Trichoderma spp. have been extensively explored and used in agriculture due to their well-known biological control mechanisms. Fungi of the genus Trichoderma produce secondary metabolites which affect plant metabolism by stimulating the production of defense-related compounds and increasing the antioxidant capacity through enhanced polyphenol content and elevated activity of antioxidant enzymes, a result of the excess production of reactive oxygen species. In this work, Nepeta sibirica plants were grown in vitro on solid medium treated with two Trichoderma strains (T. viride and T. harzianum). After three and six days of treatment, leaves were metabolically profiled for major phenolic acids (chlorogenic, caffeic, and rosmarinic acid), and iridoids content (cis,trans-nepetalactone and 1,5,9-epideoxyloganic acid), in parallel with the expression analysis of nepetalactone biosynthetic genes and regulatory genetic elements-transcription factors (TFs). Both fungal strains induced changes in phenolic acids production, while only T. harzianum induced elevated levels of iridoids. Biosynthetic genes GPPS, and IS, as well as TF MYC2, were the only genes with expression levels not affected by the treatments with micro fungi. The response of antioxidant enzymes in N. sibirica leaves was also studied. The most active antioxidant enzyme following the infection with T. viride and T. harzianum was peroxidase (POX). Catalase (CAT) and superoxide dismutase (SOD) activity were also affected by the treatments. Results indicate a possibility of using Trichoderma infection in N. sibirica to elicit the production of biologically active defense compounds.",
publisher = "Belgrade : Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Alterations in specialized metabolism and antioxidant capacity of Nepeta sibirica L. as induced by two Trichoderma sp.",
pages = "77",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5320"
}

Aničić, N., Matekalo, D., Stojković, D., Dmitrović, S., Nestorović Živković, J., Skorić, M., Gašić, U., Milutinović, M., Petrović, L., Božunović, J., Filipović, B., Banjanac, T., Šiler, B., Todorović, M., Lukić, T.,& Mišić, D.. (2022). Alterations in specialized metabolism and antioxidant capacity of Nepeta sibirica L. as induced by two Trichoderma sp.. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade : Serbian Plant Physiology Society., 77.
https://hdl.handle.net/21.15107/rcub_ibiss_5320

Aničić N, Matekalo D, Stojković D, Dmitrović S, Nestorović Živković J, Skorić M, Gašić U, Milutinović M, Petrović L, Božunović J, Filipović B, Banjanac T, Šiler B, Todorović M, Lukić T, Mišić D. Alterations in specialized metabolism and antioxidant capacity of Nepeta sibirica L. as induced by two Trichoderma sp.. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:77.
https://hdl.handle.net/21.15107/rcub_ibiss_5320 .

Aničić, Neda, Matekalo, Dragana, Stojković, Dejan, Dmitrović, Slavica, Nestorović Živković, Jasmina, Skorić, Marijana, Gašić, Uroš, Milutinović, Milica, Petrović, Luka, Božunović, Jelena, Filipović, Biljana, Banjanac, Tijana, Šiler, Branislav, Todorović, Miloš, Lukić, Tamara, Mišić, Danijela, "Alterations in specialized metabolism and antioxidant capacity of Nepeta sibirica L. as induced by two Trichoderma sp." in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):77,
https://hdl.handle.net/21.15107/rcub_ibiss_5320 .

TY  - JOUR
AU  - Garcia-Oliveira, Paula
AU  - Carreira-Casais, Anxo
AU  - Pereira, Eliana
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Simal-Gandara, Jesus
AU  - Prieto, Miguel A
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9571014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5170
AB  - Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.
PB  - Basel: MDPI
T2  - Molecules
T1  - From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants
IS  - 19
VL  - 27
DO  - 10.3390/molecules27196495
ER  - 

@article{
author = "Garcia-Oliveira, Paula and Carreira-Casais, Anxo and Pereira, Eliana and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C and Stojković, Dejan and Soković, Marina and Simal-Gandara, Jesus and Prieto, Miguel A and Caleja, Cristina and Barros, Lillian",
year = "2022",
abstract = "Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants",
number = "19",
volume = "27",
doi = "10.3390/molecules27196495"
}

Garcia-Oliveira, P., Carreira-Casais, A., Pereira, E., Dias, M. I., Pereira, C., Calhelha, R. C., Stojković, D., Soković, M., Simal-Gandara, J., Prieto, M. A., Caleja, C.,& Barros, L.. (2022). From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules
Basel: MDPI., 27(19).
https://doi.org/10.3390/molecules27196495

Garcia-Oliveira P, Carreira-Casais A, Pereira E, Dias MI, Pereira C, Calhelha RC, Stojković D, Soković M, Simal-Gandara J, Prieto MA, Caleja C, Barros L. From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules. 2022;27(19).
doi:10.3390/molecules27196495 .

Garcia-Oliveira, Paula, Carreira-Casais, Anxo, Pereira, Eliana, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C, Stojković, Dejan, Soković, Marina, Simal-Gandara, Jesus, Prieto, Miguel A, Caleja, Cristina, Barros, Lillian, "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants" in Molecules, 27, no. 19 (2022),
https://doi.org/10.3390/molecules27196495 . .

TY  - THES
AU  - Stojković, Dejan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4974
AB  - Upotreba biljaka u lečenju ljudi datira još od antičkih vremena. Danas, savremena nauka izučava lekovita svojstva biljaka, potvrđuje njihov etnobotanički značaj i opisuje nove upotrebe biljnih vrsta kao medicinskih sredstava. Cilj ove doktorske disertacije je analiza biološkog potencijala i hemijskog profila metanolnih ekstrakata dobijenih iz nadzemnih delova biljaka Anthriscus cerefolium, Ononis spinosa i Phlomis fruticosa. Biološki potencijal metanolnih ekstrakata je određen testiranjem antibakterijske, antifungalne, antibiofilm, antioksidativne, enzim-inhibitorne, antiproliferativne i anti-inflamatorne aktivnosti. Predstavljen je i uvid u mehanizme antibakterijskog, antifungalnog i antiproliferativnog delovanja ekstrakata. Dobijeni rezultati su ukazali na značajnu antimikrobnu aktivnost ekstrakata protiv patogenih bakterija i gljiva. Takođe, ekstrakti su posedovali antibiofilm aktivnost protiv biofilma bakterija i patogenih kvasnica, kao i aktivnost na neke od faktora virulencije bakterija i kvasnica. Dalje, uočen je značajan antioksidativni potencijal svih ekstrakata, kao i njihova sposobnost inhibicije medicinski važnih enzima. Testirani ekstrakti nisu pokazali toksičnost prema ne-tumorskim humanim primarnim ćelijama, ali su pokazali antiproliferativno dejstvo na tumorske ćelijske linije. Takođe, uočene su i promene morfologije ćelija glioblastoma tretiranih ekstraktom A. cerefoilum. Ekstrakti su pokazali anti-inflamatornu aktivnost na in vitro modelu bakterijski indukovane inflamacije kod humanih keratinocita kože. Takođe, u okviru ove doktorske disertacije rasvetljen je hemijski sastav polifenola u testiranim ekstraktima i utvrđeno je da dominiraju derivati fenolnih kiselina i flavonoida.
AB  - The use of plants in ethnomedicine dates back to ancient times. Today, modern science explores the healing properties of plants, confirms their ethnobotanical significance and describes new uses of plant species as medicinal agents. The aim of this dissertation is to analyze the biological potential and chemical profile of methanolic extracts obtained from the herbs Anthriscus cerefolium, Ononis spinosa and Phlomis fruticosa. The biological potential of methanolic extracts was determined by testing antibacterial, antifungal, antibiofilm, antioxidant, enzyme-inhibitory, antiproliferative and anti-inflammatory activities. An insight into the mechanisms of antibacterial, antifungal and antiproliferative actions was also presented. The chemical composition of methanol extracts has been studied both qualitatively and quantitatively. The obtained results indicated significant antimicrobial activity of the extracts against pathogenic bacteria and fungi. Also, the extracts possessed antibiofilm activity against bacterial and yeasts biofilms, as well as the activity on some of the virulence factors of bacteria and yeasts. Furthermore, significant antioxidant potential of all extracts was observed, as well as their ability to inhibit medically important enzymes. The extracts did not show toxicity to human primary cells, but showed antiproliferative effect on tested tumor cell lines. Also, the changes in the morphology of glioblastoma cells treated with the methanol extract of A. cerefoilum were observed. The extracts showed anti-inflammatory activity in in vitro model of bacterial-induced inflammation of human skin keratinocytes. Furthermore, the chemical composition of polyphenols in the tested extracts was clarified and was determined that derivatives of phenolic acids and flavonoids dominate.
PB  - Belgrade: Faculty of Biology, University of Belgrade
T2  - Faculty of Biology, University of Belgrade
T1  - Biološki potencijal i hemijski profil metanolnih ekstrakata biljaka Anthriscus cerefolium (L.) Hoffm. (Apiaceae), Ononis spinosa L. (Fabaceae) i Phlomis fruticosa L. (Lamiaceae)
T1  - Biological potential and chemical profile of methanolic extracts of herbs Anthriscus cerefolium (L.) Hoffm. (Apiaceae), Ononis spinosa L. (Fabaceae) and Phlomis fruticosa L. (Lamiaceae)
SP  - 1
EP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4974
ER  - 

@phdthesis{
author = "Stojković, Dejan",
year = "2022",
abstract = "Upotreba biljaka u lečenju ljudi datira još od antičkih vremena. Danas, savremena nauka izučava lekovita svojstva biljaka, potvrđuje njihov etnobotanički značaj i opisuje nove upotrebe biljnih vrsta kao medicinskih sredstava. Cilj ove doktorske disertacije je analiza biološkog potencijala i hemijskog profila metanolnih ekstrakata dobijenih iz nadzemnih delova biljaka Anthriscus cerefolium, Ononis spinosa i Phlomis fruticosa. Biološki potencijal metanolnih ekstrakata je određen testiranjem antibakterijske, antifungalne, antibiofilm, antioksidativne, enzim-inhibitorne, antiproliferativne i anti-inflamatorne aktivnosti. Predstavljen je i uvid u mehanizme antibakterijskog, antifungalnog i antiproliferativnog delovanja ekstrakata. Dobijeni rezultati su ukazali na značajnu antimikrobnu aktivnost ekstrakata protiv patogenih bakterija i gljiva. Takođe, ekstrakti su posedovali antibiofilm aktivnost protiv biofilma bakterija i patogenih kvasnica, kao i aktivnost na neke od faktora virulencije bakterija i kvasnica. Dalje, uočen je značajan antioksidativni potencijal svih ekstrakata, kao i njihova sposobnost inhibicije medicinski važnih enzima. Testirani ekstrakti nisu pokazali toksičnost prema ne-tumorskim humanim primarnim ćelijama, ali su pokazali antiproliferativno dejstvo na tumorske ćelijske linije. Takođe, uočene su i promene morfologije ćelija glioblastoma tretiranih ekstraktom A. cerefoilum. Ekstrakti su pokazali anti-inflamatornu aktivnost na in vitro modelu bakterijski indukovane inflamacije kod humanih keratinocita kože. Takođe, u okviru ove doktorske disertacije rasvetljen je hemijski sastav polifenola u testiranim ekstraktima i utvrđeno je da dominiraju derivati fenolnih kiselina i flavonoida., The use of plants in ethnomedicine dates back to ancient times. Today, modern science explores the healing properties of plants, confirms their ethnobotanical significance and describes new uses of plant species as medicinal agents. The aim of this dissertation is to analyze the biological potential and chemical profile of methanolic extracts obtained from the herbs Anthriscus cerefolium, Ononis spinosa and Phlomis fruticosa. The biological potential of methanolic extracts was determined by testing antibacterial, antifungal, antibiofilm, antioxidant, enzyme-inhibitory, antiproliferative and anti-inflammatory activities. An insight into the mechanisms of antibacterial, antifungal and antiproliferative actions was also presented. The chemical composition of methanol extracts has been studied both qualitatively and quantitatively. The obtained results indicated significant antimicrobial activity of the extracts against pathogenic bacteria and fungi. Also, the extracts possessed antibiofilm activity against bacterial and yeasts biofilms, as well as the activity on some of the virulence factors of bacteria and yeasts. Furthermore, significant antioxidant potential of all extracts was observed, as well as their ability to inhibit medically important enzymes. The extracts did not show toxicity to human primary cells, but showed antiproliferative effect on tested tumor cell lines. Also, the changes in the morphology of glioblastoma cells treated with the methanol extract of A. cerefoilum were observed. The extracts showed anti-inflammatory activity in in vitro model of bacterial-induced inflammation of human skin keratinocytes. Furthermore, the chemical composition of polyphenols in the tested extracts was clarified and was determined that derivatives of phenolic acids and flavonoids dominate.",
publisher = "Belgrade: Faculty of Biology, University of Belgrade",
journal = "Faculty of Biology, University of Belgrade",
title = "Biološki potencijal i hemijski profil metanolnih ekstrakata biljaka Anthriscus cerefolium (L.) Hoffm. (Apiaceae), Ononis spinosa L. (Fabaceae) i Phlomis fruticosa L. (Lamiaceae), Biological potential and chemical profile of methanolic extracts of herbs Anthriscus cerefolium (L.) Hoffm. (Apiaceae), Ononis spinosa L. (Fabaceae) and Phlomis fruticosa L. (Lamiaceae)",
pages = "1-120",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4974"
}

Stojković, D.. (2022). Biološki potencijal i hemijski profil metanolnih ekstrakata biljaka Anthriscus cerefolium (L.) Hoffm. (Apiaceae), Ononis spinosa L. (Fabaceae) i Phlomis fruticosa L. (Lamiaceae). in Faculty of Biology, University of Belgrade
Belgrade: Faculty of Biology, University of Belgrade., 1-120.
https://hdl.handle.net/21.15107/rcub_ibiss_4974

Stojković D. Biološki potencijal i hemijski profil metanolnih ekstrakata biljaka Anthriscus cerefolium (L.) Hoffm. (Apiaceae), Ononis spinosa L. (Fabaceae) i Phlomis fruticosa L. (Lamiaceae). in Faculty of Biology, University of Belgrade. 2022;:1-120.
https://hdl.handle.net/21.15107/rcub_ibiss_4974 .

Stojković, Dejan, "Biološki potencijal i hemijski profil metanolnih ekstrakata biljaka Anthriscus cerefolium (L.) Hoffm. (Apiaceae), Ononis spinosa L. (Fabaceae) i Phlomis fruticosa L. (Lamiaceae)" in Faculty of Biology, University of Belgrade (2022):1-120,
https://hdl.handle.net/21.15107/rcub_ibiss_4974 .

TY  - CONF
AU  - Stojković, Dejan
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Petrović, Jovana
AU  - Kostić, Marina
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5158
AB  - Chicory (Cichorium intybus L.) is an important industrial crop cultivated mainly to extract the
dietary fiber inulin. This study aimed to investigate antimicrobial, antibiofilm and wound healing
potentials of the extract obtained by supercritical fluid extraction (SFE). Antibacterial activity
of extract ranged from 0.25 to 4.00 mg/mL regarding minimal inhibitory concentra-tions (MICs),
while minimal bactericidal concentrations (MBCs) were noted within the range of 0.5 to 8.00 mg/
mL. The extract has shown promising antifungal potential with MICs rang-ing 0.125- 0.75 mg/
mL and MFCs 0.25-1.0 mg/mL against Candida species. Incubation with the extract has reduced
the ability of both C. albicans and P. aeruginosa to attach to the sur-face and begin the process
of biofilm formation. Concentration equal to previously deter-mined MIC has almost completely
(>90% inhibition) reduced the biofilm biomass formed by P. aeruginosa. Even twice as lower concentrations
(0.5 MIC) has limited the biofilm forming ability and induce more than 80% inhibition
in P. aeruginosa as bacterial biofilm model or-ganism. Impact on fungal biofilm was less profound
and MIC concentration was able to re-duce the biofilm formation for less than 60%. HaCaT (human
immortalized keratinocytes cell line) cells were treated with IC20 (24 h) concentration of chicory
SFE extract. It is estimated that SFE extract has slight effect on wound closure in keratinocyte
monolayer, when com-pared to untreated control. Since, Chicory SFE fraction possessed excellent
antibacterial po-tential against E. coli and K. pneumoniae and antifungal activity against Candida
species these results are considered very important in the future inventions
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5158
ER  - 

@conference{
author = "Stojković, Dejan and Ivanov, Marija and Ćirić, Ana and Petrović, Jovana and Kostić, Marina and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Chicory (Cichorium intybus L.) is an important industrial crop cultivated mainly to extract the
dietary fiber inulin. This study aimed to investigate antimicrobial, antibiofilm and wound healing
potentials of the extract obtained by supercritical fluid extraction (SFE). Antibacterial activity
of extract ranged from 0.25 to 4.00 mg/mL regarding minimal inhibitory concentra-tions (MICs),
while minimal bactericidal concentrations (MBCs) were noted within the range of 0.5 to 8.00 mg/
mL. The extract has shown promising antifungal potential with MICs rang-ing 0.125- 0.75 mg/
mL and MFCs 0.25-1.0 mg/mL against Candida species. Incubation with the extract has reduced
the ability of both C. albicans and P. aeruginosa to attach to the sur-face and begin the process
of biofilm formation. Concentration equal to previously deter-mined MIC has almost completely
(>90% inhibition) reduced the biofilm biomass formed by P. aeruginosa. Even twice as lower concentrations
(0.5 MIC) has limited the biofilm forming ability and induce more than 80% inhibition
in P. aeruginosa as bacterial biofilm model or-ganism. Impact on fungal biofilm was less profound
and MIC concentration was able to re-duce the biofilm formation for less than 60%. HaCaT (human
immortalized keratinocytes cell line) cells were treated with IC20 (24 h) concentration of chicory
SFE extract. It is estimated that SFE extract has slight effect on wound closure in keratinocyte
monolayer, when com-pared to untreated control. Since, Chicory SFE fraction possessed excellent
antibacterial po-tential against E. coli and K. pneumoniae and antifungal activity against Candida
species these results are considered very important in the future inventions",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5158"
}

Stojković, D., Ivanov, M., Ćirić, A., Petrović, J., Kostić, M., Glamočlija, J.,& Soković, M.. (2022). Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5158

Stojković D, Ivanov M, Ćirić A, Petrović J, Kostić M, Glamočlija J, Soković M. Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5158 .

Stojković, Dejan, Ivanov, Marija, Ćirić, Ana, Petrović, Jovana, Kostić, Marina, Glamočlija, Jasmina, Soković, Marina, "Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5158 .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Kostić, Marina
AU  - Stojković, Dejan
AU  - Soković, Marina
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S025462992100497X
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4748
AB  - Emerging antimicrobial resistance and side effects associated with antibiotics overuse are driving the need for the novel antimicrobial therapeutics with the natural compounds considered as an appealing alternative. Rosmarinic acid is abundantly present in medicinal plants. This study aimed to elucidate its possible role as an inhibitor of planktonic and biofilm microorganism growth, along with its antifungal mechanisms. In this study, rosmarinic acid showed promising anticandidal (MIC 0.1–0.2 mg/mL) and antibacterial (MIC 0.002->0.8 mg/mL) activity. To some extent it was able to prevent cell attachment and eradicate established biofilms. Rosmarinic acid antifungal mechanisms included reduction of mitochondrial activity, alteration in membrane integrity, and slight inhibition of protease production but not binding to ergosterol. Its antibiofilm activity was moderately linked to the reduction in exopolysaccharide production. Considering its wide antimicrobial and antibiofilm spectrum rosmarinic acid could be further explored as an antimicrobial agent along with range of medicinal plants with rosmarinic acid as the dominant compound. Nevertheless, rosmarinic acid could serve as the basis for the development of antimicrobials employing novel mechanisms of activity.
T2  - South African Journal of Botany
T1  - Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol
VL  - 146
DO  - 10.1016/j.sajb.2021.11.050
SP  - 521
EP  - 527
ER  - 

@article{
author = "Ivanov, Marija and Kostić, Marina and Stojković, Dejan and Soković, Marina",
year = "2022",
abstract = "Emerging antimicrobial resistance and side effects associated with antibiotics overuse are driving the need for the novel antimicrobial therapeutics with the natural compounds considered as an appealing alternative. Rosmarinic acid is abundantly present in medicinal plants. This study aimed to elucidate its possible role as an inhibitor of planktonic and biofilm microorganism growth, along with its antifungal mechanisms. In this study, rosmarinic acid showed promising anticandidal (MIC 0.1–0.2 mg/mL) and antibacterial (MIC 0.002->0.8 mg/mL) activity. To some extent it was able to prevent cell attachment and eradicate established biofilms. Rosmarinic acid antifungal mechanisms included reduction of mitochondrial activity, alteration in membrane integrity, and slight inhibition of protease production but not binding to ergosterol. Its antibiofilm activity was moderately linked to the reduction in exopolysaccharide production. Considering its wide antimicrobial and antibiofilm spectrum rosmarinic acid could be further explored as an antimicrobial agent along with range of medicinal plants with rosmarinic acid as the dominant compound. Nevertheless, rosmarinic acid could serve as the basis for the development of antimicrobials employing novel mechanisms of activity.",
journal = "South African Journal of Botany",
title = "Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol",
volume = "146",
doi = "10.1016/j.sajb.2021.11.050",
pages = "521-527"
}

Ivanov, M., Kostić, M., Stojković, D.,& Soković, M.. (2022). Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol. in South African Journal of Botany, 146, 521-527.
https://doi.org/10.1016/j.sajb.2021.11.050

Ivanov M, Kostić M, Stojković D, Soković M. Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol. in South African Journal of Botany. 2022;146:521-527.
doi:10.1016/j.sajb.2021.11.050 .

Ivanov, Marija, Kostić, Marina, Stojković, Dejan, Soković, Marina, "Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol" in South African Journal of Botany, 146 (2022):521-527,
https://doi.org/10.1016/j.sajb.2021.11.050 . .

TY  - CONF
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Kostić, Marina
AU  - Petrović, Jovana
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5581
AB  - Cichorium intybus L. (Asteraceae), is an important medicinal plant, rich in sesquiterpene
lactones (STLs), inulin, biologically relevant elements, and vitamins which exert potent
pro-health effects on the human organism. The aim of this study was to screen two
extracts of chicory containing STLs, the optimized extract (Ci_SFEopt) and the
supercritical fluid extract (Ci_SFEpur), for their antimicrobial activities against a range
of pathogenic microorganisms. Both extracts showed antibacterial activity, with
Ci_SFEpur being more potent than Ci_SFEopt. The most sensitive species was
Pseudomonas aeruginosa (minimal inhibitory concentration, MIC 0.06 mg/mL).
Ci_SFEpur and Ci_SFEopt extracts demonstrated good antifungal activity against
Candida species (MIC 0.03-1.00 mg/mL). Thin layer chromatography (TLC)
bioautography was used to screen the extracts and provide basic information for the
isolation of bioactive compounds in the future. Ci_SFEopt was active against all tested
microorganisms (Rf values were 0.18-0.88). The Ci_SFEpur extract efficiently inhibited
(Rf 0.76) Staphylococcus aureus, P. aeruginosa and Candida krusei. The identification
of powerful antimicrobial activity of Ci_SFEpur against P. aeruginosa and Candida
species is an important step towards the development of novel antimicrobial therapeutics
and efficient utilization of this widely distributed plant.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Evaluation of antimicrobial properties of chicory extracts
SP  - 257
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5581
ER  - 

@conference{
author = "Ćirić, Ana and Ivanov, Marija and Stojković, Dejan and Glamočlija, Jasmina and Kostić, Marina and Petrović, Jovana and Soković, Marina",
year = "2022",
abstract = "Cichorium intybus L. (Asteraceae), is an important medicinal plant, rich in sesquiterpene
lactones (STLs), inulin, biologically relevant elements, and vitamins which exert potent
pro-health effects on the human organism. The aim of this study was to screen two
extracts of chicory containing STLs, the optimized extract (Ci_SFEopt) and the
supercritical fluid extract (Ci_SFEpur), for their antimicrobial activities against a range
of pathogenic microorganisms. Both extracts showed antibacterial activity, with
Ci_SFEpur being more potent than Ci_SFEopt. The most sensitive species was
Pseudomonas aeruginosa (minimal inhibitory concentration, MIC 0.06 mg/mL).
Ci_SFEpur and Ci_SFEopt extracts demonstrated good antifungal activity against
Candida species (MIC 0.03-1.00 mg/mL). Thin layer chromatography (TLC)
bioautography was used to screen the extracts and provide basic information for the
isolation of bioactive compounds in the future. Ci_SFEopt was active against all tested
microorganisms (Rf values were 0.18-0.88). The Ci_SFEpur extract efficiently inhibited
(Rf 0.76) Staphylococcus aureus, P. aeruginosa and Candida krusei. The identification
of powerful antimicrobial activity of Ci_SFEpur against P. aeruginosa and Candida
species is an important step towards the development of novel antimicrobial therapeutics
and efficient utilization of this widely distributed plant.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Evaluation of antimicrobial properties of chicory extracts",
pages = "257",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5581"
}

Ćirić, A., Ivanov, M., Stojković, D., Glamočlija, J., Kostić, M., Petrović, J.,& Soković, M.. (2022). Evaluation of antimicrobial properties of chicory extracts. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 257.
https://hdl.handle.net/21.15107/rcub_ibiss_5581

Ćirić A, Ivanov M, Stojković D, Glamočlija J, Kostić M, Petrović J, Soković M. Evaluation of antimicrobial properties of chicory extracts. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:257.
https://hdl.handle.net/21.15107/rcub_ibiss_5581 .

Ćirić, Ana, Ivanov, Marija, Stojković, Dejan, Glamočlija, Jasmina, Kostić, Marina, Petrović, Jovana, Soković, Marina, "Evaluation of antimicrobial properties of chicory extracts" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):257,
https://hdl.handle.net/21.15107/rcub_ibiss_5581 .

TY  - CONF
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Caleja, Cristina
AU  - Pereira, Eliana
AU  - Barros, Lillian
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5156
AB  - Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Chemical composition and biological activity of two Lactarius species from Serbia.
SP  - 102
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5156
ER  - 

@conference{
author = "Kostić, Marina and Ivanov, Marija and Ćirić, Ana and Caleja, Cristina and Pereira, Eliana and Barros, Lillian and Stojković, Dejan and Soković, Marina and Glamočlija, Jasmina",
year = "2022",
abstract = "Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Chemical composition and biological activity of two Lactarius species from Serbia.",
pages = "102-103",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5156"
}

Kostić, M., Ivanov, M., Ćirić, A., Caleja, C., Pereira, E., Barros, L., Stojković, D., Soković, M.,& Glamočlija, J.. (2022). Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156

Kostić M, Ivanov M, Ćirić A, Caleja C, Pereira E, Barros L, Stojković D, Soković M, Glamočlija J. Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

Kostić, Marina, Ivanov, Marija, Ćirić, Ana, Caleja, Cristina, Pereira, Eliana, Barros, Lillian, Stojković, Dejan, Soković, Marina, Glamočlija, Jasmina, "Chemical composition and biological activity of two Lactarius species from Serbia." in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):102-103,
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

TY  - JOUR
AU  - Carević, Tamara
AU  - Kostić, Marina
AU  - Nikolić, Biljana
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ivanov, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5146
AB  - Hesperetin is the aglycone of citrus flavonoid hesperidin. Due to the limited information regarding hesperetin antimicrobial potential and emerging need for novel antimicrobials, we have studied its antimicrobial activity (microdilution assay), antibiofilm activity with different assays in two models (mono- and polymicrobial biofilm), and toxicity (MTT and brine shrimp lethality assays). Hesperetin inhibited growth of all Candida isolates (minimal inhibitory concentration, MIC, 0.165 mg/mL), while it’s inhibitory potential towards Staphylococcus aureus was lower (MIC 4 mg/mL). Hesperetin (0.165 mg/mL) reduced ability of Candida to form biofilms and moderately reduced exopolysaccharide levels in biofilm matrix. Effect on the eradication of 24 h old C. albicans biofilms was promising at 1.320 mg/mL. Inhibition of staphylococcal biofilm formation required higher concentrations of hesperetin (<50% inhibition with MIC 4 mg/mL). Establishment of polymicrobial C. albicans-S. aureus biofilm was significantly inhibited with the lowest examined hesperetin concentration (1 mg/mL) in crystal violet and CFU assays. Hesperetin toxicity was examined towards MRC-5 fibroblasts (IC50 0.340 mg/mL) and in brine shrimp lethality assay (LC50 > 1 mg/mL). Hesperetin is efficient in combating growth and biofilm formation of Candida species. However, its antibacterial application should be further examined due to the cytotoxic effects provoked in the antibacterial concentrations
PB  - Basel: MDPI
T2  - Molecules
T1  - Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity
IS  - 20
VL  - 27
DO  - 10.3390/molecules27206806
SP  - 6806
ER  - 

@article{
author = "Carević, Tamara and Kostić, Marina and Nikolić, Biljana and Stojković, Dejan and Soković, Marina and Ivanov, Marija",
year = "2022",
abstract = "Hesperetin is the aglycone of citrus flavonoid hesperidin. Due to the limited information regarding hesperetin antimicrobial potential and emerging need for novel antimicrobials, we have studied its antimicrobial activity (microdilution assay), antibiofilm activity with different assays in two models (mono- and polymicrobial biofilm), and toxicity (MTT and brine shrimp lethality assays). Hesperetin inhibited growth of all Candida isolates (minimal inhibitory concentration, MIC, 0.165 mg/mL), while it’s inhibitory potential towards Staphylococcus aureus was lower (MIC 4 mg/mL). Hesperetin (0.165 mg/mL) reduced ability of Candida to form biofilms and moderately reduced exopolysaccharide levels in biofilm matrix. Effect on the eradication of 24 h old C. albicans biofilms was promising at 1.320 mg/mL. Inhibition of staphylococcal biofilm formation required higher concentrations of hesperetin (<50% inhibition with MIC 4 mg/mL). Establishment of polymicrobial C. albicans-S. aureus biofilm was significantly inhibited with the lowest examined hesperetin concentration (1 mg/mL) in crystal violet and CFU assays. Hesperetin toxicity was examined towards MRC-5 fibroblasts (IC50 0.340 mg/mL) and in brine shrimp lethality assay (LC50 > 1 mg/mL). Hesperetin is efficient in combating growth and biofilm formation of Candida species. However, its antibacterial application should be further examined due to the cytotoxic effects provoked in the antibacterial concentrations",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity",
number = "20",
volume = "27",
doi = "10.3390/molecules27206806",
pages = "6806"
}

Carević, T., Kostić, M., Nikolić, B., Stojković, D., Soković, M.,& Ivanov, M.. (2022). Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity. in Molecules
Basel: MDPI., 27(20), 6806.
https://doi.org/10.3390/molecules27206806

Carević T, Kostić M, Nikolić B, Stojković D, Soković M, Ivanov M. Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity. in Molecules. 2022;27(20):6806.
doi:10.3390/molecules27206806 .

Carević, Tamara, Kostić, Marina, Nikolić, Biljana, Stojković, Dejan, Soković, Marina, Ivanov, Marija, "Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity" in Molecules, 27, no. 20 (2022):6806,
https://doi.org/10.3390/molecules27206806 . .

TY  - JOUR
AU  - Moreira, Bruna
AU  - Pereira, Eliana
AU  - Finimundy, Tiane C.
AU  - Pinela, José
AU  - Calhelha, Ricardo C.
AU  - Carocho, Márcio
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2022
UR  - http://xlink.rsc.org/?DOI=D2FO00657J
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5120
AB  - Pineapple is a tropical fruit consumed fresh or processed into various food products. However, the peel and crown of this fruit are not industrially exploited, thus generating tons of by-products that represent an economic and environmental concern. In order to promote the upcycling of these by-products, this work aimed to characterize the phenolic profile of its hydroethanolic extracts obtained from pineapple peel and crown leaves and to evaluate their in vitro bioactivity. The HPLC-DAD-ESI/MS analysis allowed the identification of 25 phenolic compounds, including phenolic acids and flavonoids. The antioxidant, cytotoxic, and antimicrobial activity assays highlighted the peel extract as the most promising and, therefore, it was incorporated into a traditional Portuguese pastry cake as a functional ingredient. The nutritional parameters of the developed food were not affected by the incorporation of the extract, but it promoted the antioxidant activity during its shelf-life. Overall, pineapple peel and crown appeared as promising by-products to be exploited by the food industry, which can be achieved through a circular economy approach.
PB  - Cambridge: Royal Society of Chemistry
T2  - Food & Function
T1  - Pineapple by-products as a source of bioactive compounds with potential for industrial food application.
IS  - 19
VL  - 13
DO  - 10.1039/d2fo00657j
SP  - 9959
EP  - 9972
ER  - 

@article{
author = "Moreira, Bruna and Pereira, Eliana and Finimundy, Tiane C. and Pinela, José and Calhelha, Ricardo C. and Carocho, Márcio and Stojković, Dejan and Soković, Marina and Ferreira, Isabel C. F. R. and Caleja, Cristina and Barros, Lillian",
year = "2022",
abstract = "Pineapple is a tropical fruit consumed fresh or processed into various food products. However, the peel and crown of this fruit are not industrially exploited, thus generating tons of by-products that represent an economic and environmental concern. In order to promote the upcycling of these by-products, this work aimed to characterize the phenolic profile of its hydroethanolic extracts obtained from pineapple peel and crown leaves and to evaluate their in vitro bioactivity. The HPLC-DAD-ESI/MS analysis allowed the identification of 25 phenolic compounds, including phenolic acids and flavonoids. The antioxidant, cytotoxic, and antimicrobial activity assays highlighted the peel extract as the most promising and, therefore, it was incorporated into a traditional Portuguese pastry cake as a functional ingredient. The nutritional parameters of the developed food were not affected by the incorporation of the extract, but it promoted the antioxidant activity during its shelf-life. Overall, pineapple peel and crown appeared as promising by-products to be exploited by the food industry, which can be achieved through a circular economy approach.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "Food & Function",
title = "Pineapple by-products as a source of bioactive compounds with potential for industrial food application.",
number = "19",
volume = "13",
doi = "10.1039/d2fo00657j",
pages = "9959-9972"
}

Moreira, B., Pereira, E., Finimundy, T. C., Pinela, J., Calhelha, R. C., Carocho, M., Stojković, D., Soković, M., Ferreira, I. C. F. R., Caleja, C.,& Barros, L.. (2022). Pineapple by-products as a source of bioactive compounds with potential for industrial food application.. in Food & Function
Cambridge: Royal Society of Chemistry., 13(19), 9959-9972.
https://doi.org/10.1039/d2fo00657j

Moreira B, Pereira E, Finimundy TC, Pinela J, Calhelha RC, Carocho M, Stojković D, Soković M, Ferreira ICFR, Caleja C, Barros L. Pineapple by-products as a source of bioactive compounds with potential for industrial food application.. in Food & Function. 2022;13(19):9959-9972.
doi:10.1039/d2fo00657j .

Moreira, Bruna, Pereira, Eliana, Finimundy, Tiane C., Pinela, José, Calhelha, Ricardo C., Carocho, Márcio, Stojković, Dejan, Soković, Marina, Ferreira, Isabel C. F. R., Caleja, Cristina, Barros, Lillian, "Pineapple by-products as a source of bioactive compounds with potential for industrial food application." in Food & Function, 13, no. 19 (2022):9959-9972,
https://doi.org/10.1039/d2fo00657j . .