TY  - JOUR
AU  - Marlani, Maia
AU  - Nadaraia, Nanuli
AU  - Barbakadze, Nana
AU  - Amiranashvili, Lela
AU  - Kakhabrishvili, M
AU  - Petrou, Anthi
AU  - Carević, Tamara
AU  - Glamočlija, Jasmina
AU  - Geronikaki, Athina
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6556
AB  - Most of pharmaceutical agents display several or even many biological activities. It is obvious that testing even one compound for thousands of biological activities is a practically not reasonable task. Therefore, computer-aided prediction is the method of choice for the selection of the most promising bioassays for particular compounds. Using PASS Online software, we determined the probable antimicrobial activity of the 31 steroid derivatives. Experimental testing of the antimicrobial activity of the tested compounds by microdilution method confirmed the computational predictions. Furthermore, P. aeruginosa and C. albicans biofilm formation was investigated. Compound 11 showed a biofilm reduction by 42.26% at the MIC of the tested compound. The percentages are lower than ketoconazole, but very close to its activity.
PB  - Abingdon: Taylor and Francis
T2  - SAR and QSAR in Environmental Research
T1  - Steroidal hydrazones as antimicrobial agents: biological evaluation and molecular docking studies
IS  - 2
VL  - 35
DO  - 10.1080/1062936X.2024.2309183
SP  - 137
EP  - 155
ER  - 

@article{
author = "Marlani, Maia and Nadaraia, Nanuli and Barbakadze, Nana and Amiranashvili, Lela and Kakhabrishvili, M and Petrou, Anthi and Carević, Tamara and Glamočlija, Jasmina and Geronikaki, Athina",
year = "2024",
abstract = "Most of pharmaceutical agents display several or even many biological activities. It is obvious that testing even one compound for thousands of biological activities is a practically not reasonable task. Therefore, computer-aided prediction is the method of choice for the selection of the most promising bioassays for particular compounds. Using PASS Online software, we determined the probable antimicrobial activity of the 31 steroid derivatives. Experimental testing of the antimicrobial activity of the tested compounds by microdilution method confirmed the computational predictions. Furthermore, P. aeruginosa and C. albicans biofilm formation was investigated. Compound 11 showed a biofilm reduction by 42.26% at the MIC of the tested compound. The percentages are lower than ketoconazole, but very close to its activity.",
publisher = "Abingdon: Taylor and Francis",
journal = "SAR and QSAR in Environmental Research",
title = "Steroidal hydrazones as antimicrobial agents: biological evaluation and molecular docking studies",
number = "2",
volume = "35",
doi = "10.1080/1062936X.2024.2309183",
pages = "137-155"
}

Marlani, M., Nadaraia, N., Barbakadze, N., Amiranashvili, L., Kakhabrishvili, M., Petrou, A., Carević, T., Glamočlija, J.,& Geronikaki, A.. (2024). Steroidal hydrazones as antimicrobial agents: biological evaluation and molecular docking studies. in SAR and QSAR in Environmental Research
Abingdon: Taylor and Francis., 35(2), 137-155.
https://doi.org/10.1080/1062936X.2024.2309183

Marlani M, Nadaraia N, Barbakadze N, Amiranashvili L, Kakhabrishvili M, Petrou A, Carević T, Glamočlija J, Geronikaki A. Steroidal hydrazones as antimicrobial agents: biological evaluation and molecular docking studies. in SAR and QSAR in Environmental Research. 2024;35(2):137-155.
doi:10.1080/1062936X.2024.2309183 .

Marlani, Maia, Nadaraia, Nanuli, Barbakadze, Nana, Amiranashvili, Lela, Kakhabrishvili, M, Petrou, Anthi, Carević, Tamara, Glamočlija, Jasmina, Geronikaki, Athina, "Steroidal hydrazones as antimicrobial agents: biological evaluation and molecular docking studies" in SAR and QSAR in Environmental Research, 35, no. 2 (2024):137-155,
https://doi.org/10.1080/1062936X.2024.2309183 . .

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Fernandes, Ângela
AU  - Calhelha, Ricardo C.
AU  - Glamočlija, Jasmina
AU  - Barros, Lillian
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2023
UR  - https://www.mdpi.com/2309-608X/9/1/70
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9864295
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5461
AB  - Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).
PB  - Basel: MDPI
T2  - Journal of Fungi
T1  - A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity
IS  - 1
VL  - 9
DO  - 10.3390/jof9010070
SP  - 70
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Fernandes, Ângela and Calhelha, Ricardo C. and Glamočlija, Jasmina and Barros, Lillian and Soković, Marina and Ćirić, Ana",
year = "2023",
abstract = "Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).",
publisher = "Basel: MDPI",
journal = "Journal of Fungi",
title = "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity",
number = "1",
volume = "9",
doi = "10.3390/jof9010070",
pages = "70"
}

Kostić, M., Ivanov, M., Fernandes, Â., Calhelha, R. C., Glamočlija, J., Barros, L., Soković, M.,& Ćirić, A.. (2023). A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi
Basel: MDPI., 9(1), 70.
https://doi.org/10.3390/jof9010070

Kostić M, Ivanov M, Fernandes Â, Calhelha RC, Glamočlija J, Barros L, Soković M, Ćirić A. A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi. 2023;9(1):70.
doi:10.3390/jof9010070 .

Kostić, Marina, Ivanov, Marija, Fernandes, Ângela, Calhelha, Ricardo C., Glamočlija, Jasmina, Barros, Lillian, Soković, Marina, Ćirić, Ana, "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity" in Journal of Fungi, 9, no. 1 (2023):70,
https://doi.org/10.3390/jof9010070 . .

TY  - JOUR
AU  - Merlani, Maia
AU  - Nadaraia, Nanuli
AU  - Amiranashvili, Lela
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Carević, Tamara
AU  - Soković, Marina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5659
AB  - Twelve steroid based hydrazones were in silico evaluated using computer program PASS as antimicrobial agents. The experimental evaluation revealed that all compounds have low to moderate antibacterial activity against all bacteria tested, except for B.cereus with MIC at a range of 0.37–3.00 mg/mL and MBC at 0.75–6.00 mg/mL. The most potent appeared to be compound 11 with MIC/MBC of0.75/1.5 mg/mL, respectively. The evaluation of antibacterial activity against three resistant strains MRSA, E.coli and P.aeruginosa demonstrated superior activity of compounds against MRSA compared with ampicillin, which did not show bacteriostatic or bactericidal activities. All compounds exhibited good antifungal activity with MIC of 0.37–1.50 mg/mL and MFC of 1.50–3.00 mg/mL, but with different sensitivity against fungi tested. According to docking studies, 14-alpha demethylase inhibition may be responsible for antifungal activity. Two compounds were evaluated for their antibiofilm activity. Finally, drug-likeness and docking prediction were performed.
PB  - Basel: MDPI
T2  - Molecules
T1  - Antimicrobial Activity of Some Steroidal Hydrazones
IS  - 3
VL  - 28
DO  - 10.3390/molecules28031167
SP  - 1167
ER  - 

@article{
author = "Merlani, Maia and Nadaraia, Nanuli and Amiranashvili, Lela and Petrou, Anthi and Geronikaki, Athina and Ćirić, Ana and Glamočlija, Jasmina and Carević, Tamara and Soković, Marina",
year = "2023",
abstract = "Twelve steroid based hydrazones were in silico evaluated using computer program PASS as antimicrobial agents. The experimental evaluation revealed that all compounds have low to moderate antibacterial activity against all bacteria tested, except for B.cereus with MIC at a range of 0.37–3.00 mg/mL and MBC at 0.75–6.00 mg/mL. The most potent appeared to be compound 11 with MIC/MBC of0.75/1.5 mg/mL, respectively. The evaluation of antibacterial activity against three resistant strains MRSA, E.coli and P.aeruginosa demonstrated superior activity of compounds against MRSA compared with ampicillin, which did not show bacteriostatic or bactericidal activities. All compounds exhibited good antifungal activity with MIC of 0.37–1.50 mg/mL and MFC of 1.50–3.00 mg/mL, but with different sensitivity against fungi tested. According to docking studies, 14-alpha demethylase inhibition may be responsible for antifungal activity. Two compounds were evaluated for their antibiofilm activity. Finally, drug-likeness and docking prediction were performed.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Antimicrobial Activity of Some Steroidal Hydrazones",
number = "3",
volume = "28",
doi = "10.3390/molecules28031167",
pages = "1167"
}

Merlani, M., Nadaraia, N., Amiranashvili, L., Petrou, A., Geronikaki, A., Ćirić, A., Glamočlija, J., Carević, T.,& Soković, M.. (2023). Antimicrobial Activity of Some Steroidal Hydrazones. in Molecules
Basel: MDPI., 28(3), 1167.
https://doi.org/10.3390/molecules28031167

Merlani M, Nadaraia N, Amiranashvili L, Petrou A, Geronikaki A, Ćirić A, Glamočlija J, Carević T, Soković M. Antimicrobial Activity of Some Steroidal Hydrazones. in Molecules. 2023;28(3):1167.
doi:10.3390/molecules28031167 .

Merlani, Maia, Nadaraia, Nanuli, Amiranashvili, Lela, Petrou, Anthi, Geronikaki, Athina, Ćirić, Ana, Glamočlija, Jasmina, Carević, Tamara, Soković, Marina, "Antimicrobial Activity of Some Steroidal Hydrazones" in Molecules, 28, no. 3 (2023):1167,
https://doi.org/10.3390/molecules28031167 . .

TY  - JOUR
AU  - Scanavacca, Juliana
AU  - Bortolucci, Wanessa de Campos
AU  - Jacomassi, Ezilda
AU  - Baretta, Irineia Paulina
AU  - Faria, Maria Graciela Iecher
AU  - Fernandez, Carla Maria Mariano
AU  - Tešević, Vele
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Colauto, Nelson Barros
AU  - Linde, Giani Andrea
AU  - Gazim, Zilda Cristiani
PY  - 2023
UR  - https://blacpma.ms-editions.cl/index.php/blacpma/article/view/337/345
UR  - https://blacpma.ms-editions.cl/index.php/blacpma/article/view/337
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5233
AB  - Food spoilage is a widely neglected problem and the constant use of synthetic fungicides could develop resistant fungi. The objective of this study was to evaluate the chemical composition and antimicrobial activity of Tetradenia riparia leaf essential oil against foodborne disease microorganisms. Leaf essential oil was obtained by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antimicrobial activity was studied by broth microdilution. The major compounds identified were oxygenated sesquiterpenes (43.6%): 14-hydroxy-9-epi-(E)-cariophylene (20.8%) and τ-cadinol (18.4%); followed by oxygenated diterpenes (24.6%): 6,7-dehydroroyleanone (12.6%) and 9β, 13β-epoxy-7-abiethene (10.6%); sesquiterpenic hydrocarbons (17.1%) and oxygenated monoterpenes (7.4%): fenchone (5.6%). The essential oil had broad antibacterial and antifungal activity, mainly against A. versicolor and P. ochrochloron with fungistatic and fungicidal activities and B. cereus, L. monocytogenes, and S. aureus with bacteriostatic and bactericidal activities. T. riparia leaf essential oil is a potential alternative to control microorganisms.
PB  - MS-Editions
T2  - Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
T1  - Antimicrobial activity of Tetradenia riparia leaf essential oil
IS  - 2
VL  - 22
DO  - 10.37360/blacpma.23.22.2.19
SP  - 255
EP  - 267
ER  - 

@article{
author = "Scanavacca, Juliana and Bortolucci, Wanessa de Campos and Jacomassi, Ezilda and Baretta, Irineia Paulina and Faria, Maria Graciela Iecher and Fernandez, Carla Maria Mariano and Tešević, Vele and Glamočlija, Jasmina and Soković, Marina and Colauto, Nelson Barros and Linde, Giani Andrea and Gazim, Zilda Cristiani",
year = "2023",
abstract = "Food spoilage is a widely neglected problem and the constant use of synthetic fungicides could develop resistant fungi. The objective of this study was to evaluate the chemical composition and antimicrobial activity of Tetradenia riparia leaf essential oil against foodborne disease microorganisms. Leaf essential oil was obtained by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antimicrobial activity was studied by broth microdilution. The major compounds identified were oxygenated sesquiterpenes (43.6%): 14-hydroxy-9-epi-(E)-cariophylene (20.8%) and τ-cadinol (18.4%); followed by oxygenated diterpenes (24.6%): 6,7-dehydroroyleanone (12.6%) and 9β, 13β-epoxy-7-abiethene (10.6%); sesquiterpenic hydrocarbons (17.1%) and oxygenated monoterpenes (7.4%): fenchone (5.6%). The essential oil had broad antibacterial and antifungal activity, mainly against A. versicolor and P. ochrochloron with fungistatic and fungicidal activities and B. cereus, L. monocytogenes, and S. aureus with bacteriostatic and bactericidal activities. T. riparia leaf essential oil is a potential alternative to control microorganisms.",
publisher = "MS-Editions",
journal = "Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas",
title = "Antimicrobial activity of Tetradenia riparia leaf essential oil",
number = "2",
volume = "22",
doi = "10.37360/blacpma.23.22.2.19",
pages = "255-267"
}

Scanavacca, J., Bortolucci, W. d. C., Jacomassi, E., Baretta, I. P., Faria, M. G. I., Fernandez, C. M. M., Tešević, V., Glamočlija, J., Soković, M., Colauto, N. B., Linde, G. A.,& Gazim, Z. C.. (2023). Antimicrobial activity of Tetradenia riparia leaf essential oil. in Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
MS-Editions., 22(2), 255-267.
https://doi.org/10.37360/blacpma.23.22.2.19

Scanavacca J, Bortolucci WDC, Jacomassi E, Baretta IP, Faria MGI, Fernandez CMM, Tešević V, Glamočlija J, Soković M, Colauto NB, Linde GA, Gazim ZC. Antimicrobial activity of Tetradenia riparia leaf essential oil. in Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas. 2023;22(2):255-267.
doi:10.37360/blacpma.23.22.2.19 .

Scanavacca, Juliana, Bortolucci, Wanessa de Campos, Jacomassi, Ezilda, Baretta, Irineia Paulina, Faria, Maria Graciela Iecher, Fernandez, Carla Maria Mariano, Tešević, Vele, Glamočlija, Jasmina, Soković, Marina, Colauto, Nelson Barros, Linde, Giani Andrea, Gazim, Zilda Cristiani, "Antimicrobial activity of Tetradenia riparia leaf essential oil" in Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, 22, no. 2 (2023):255-267,
https://doi.org/10.37360/blacpma.23.22.2.19 . .

TY  - JOUR
AU  - Petrou, Anthi
AU  - Kartsev, Victor
AU  - Geronikaki, Athina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Soković, Marina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5796
AB  - Nine new functionally substituted derivatives of 2-aminothiazole 
were evaluated for antimicrobial activity using microdilution 
method against the panel of eight bacterial and eight fungal strains. 
Evaluation of antibacterial activity revealed that compounds are 
potent antibacterial agents, more active than ampicillin and strep tomycin except of some compounds against B. cereus and En. 
cloacae. The best compound appeared to be compound 8. The 
most sensitive bacteria appeared to be En. cloacae, while L. mono cytogenes was the most resistant. Compounds also exhibited good 
antifungal activity much better than two reference drugs, ketoco nazole and bifonazole. Compound 1 exhibited the best antifungal 
activity. The most sensitive fungus was T. viride, while A. fumigatus 
was the most resistant. Bacteria as well as fungi in general showed 
different sensitivity towards compounds tested. Molecular docking 
studies revealed that MurB inhibition is probably involved in the 
mechanism of antibacterial activity, while CYP51 of C. albicans is 
responsible for the mechanism of antifungal activity. Finally, it 
should be mentioned that all compounds displayed very good 
druglikeness scores
PB  - Taylor & Francis Group
T2  - SAR and QSAR in Environmental Research
T1  - Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation
IS  - 5
VL  - 34
DO  - 10.1080/1062936X.2023.2214869
SP  - 359
EP  - 414
ER  - 

@article{
author = "Petrou, Anthi and Kartsev, Victor and Geronikaki, Athina and Glamočlija, Jasmina and Ćirić, Ana and Soković, Marina",
year = "2023",
abstract = "Nine new functionally substituted derivatives of 2-aminothiazole 
were evaluated for antimicrobial activity using microdilution 
method against the panel of eight bacterial and eight fungal strains. 
Evaluation of antibacterial activity revealed that compounds are 
potent antibacterial agents, more active than ampicillin and strep tomycin except of some compounds against B. cereus and En. 
cloacae. The best compound appeared to be compound 8. The 
most sensitive bacteria appeared to be En. cloacae, while L. mono cytogenes was the most resistant. Compounds also exhibited good 
antifungal activity much better than two reference drugs, ketoco nazole and bifonazole. Compound 1 exhibited the best antifungal 
activity. The most sensitive fungus was T. viride, while A. fumigatus 
was the most resistant. Bacteria as well as fungi in general showed 
different sensitivity towards compounds tested. Molecular docking 
studies revealed that MurB inhibition is probably involved in the 
mechanism of antibacterial activity, while CYP51 of C. albicans is 
responsible for the mechanism of antifungal activity. Finally, it 
should be mentioned that all compounds displayed very good 
druglikeness scores",
publisher = "Taylor & Francis Group",
journal = "SAR and QSAR in Environmental Research",
title = "Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation",
number = "5",
volume = "34",
doi = "10.1080/1062936X.2023.2214869",
pages = "359-414"
}

Petrou, A., Kartsev, V., Geronikaki, A., Glamočlija, J., Ćirić, A.,& Soković, M.. (2023). Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation. in SAR and QSAR in Environmental Research
Taylor & Francis Group., 34(5), 359-414.
https://doi.org/10.1080/1062936X.2023.2214869

Petrou A, Kartsev V, Geronikaki A, Glamočlija J, Ćirić A, Soković M. Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation. in SAR and QSAR in Environmental Research. 2023;34(5):359-414.
doi:10.1080/1062936X.2023.2214869 .

Petrou, Anthi, Kartsev, Victor, Geronikaki, Athina, Glamočlija, Jasmina, Ćirić, Ana, Soković, Marina, "Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation" in SAR and QSAR in Environmental Research, 34, no. 5 (2023):359-414,
https://doi.org/10.1080/1062936X.2023.2214869 . .

TY  - JOUR
AU  - Radovanović, Marina
AU  - Lazarević, Nevena
AU  - Novaković, Jovana
AU  - Jeremić, Nevena
AU  - Jakovljević, Vladimir
AU  - Živković, Vladimir
AU  - Bradić, Jovana
AU  - Pecarski, Danijela
AU  - Tel-Çayan, Gülsen
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Gregori, Andrej
AU  - Petrović, Jovana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5792
AB  - The present study aimed to examine the biological activity and cardioprotective potential of Trametes versicolor heteropolysaccharides (TVH) in a rat model of metabolic syndrome (MetS). This study included 40 Wistar rats divided into 5 groups: CTRL—healthy non-treated rats; MetS—non-treated rats; and H-TV, M-TV and L-TV-rats with MetS treated with either 300, 200 or 100 mg/kg TVH per os for 4 weeks. After finishing the treatment, we conducted an oral glucose tolerance test (OGTT), hemodynamic measurements and the animals were sacrificed, hearts isolated and subjected to the Langendorff technique. Blood samples were used for the determination of oxidative stress parameters, lipid status and insulin levels. We showed that α-amylase inhibition was not the mode of TVH antidiabetic action, while TVH showed a moderate inhibition of pathogenic microorganisms’ growth (MIC 8.00 mg·mL−1; MBC/MFC 16.00 mg·mL−1). H-TV and M-TV significantly reduced the level of prooxidants (O2−, H2O2, TBARS; p < 0.05), increased antioxidants activity (SOD, CAT, GSH; p < 0.05), reduced blood pressure (p < 0.05), improved glucose homeostasis in the OGTT test (p < 0.05), and ejection fraction (p < 0.05) and cardiac contractility (p < 0.05) compared to MetS (p < 0.05). Moreover, TVH treatment normalized the lipid status and decreased insulin levels compared to MetS rats (p < 0.05). The obtained results demonstrated that the TVH may be considered a useful agent for cardioprotection in MetS conditions.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome
IS  - 6
VL  - 16
DO  - 10.3390/ph16060787
SP  - 787
ER  - 

@article{
author = "Radovanović, Marina and Lazarević, Nevena and Novaković, Jovana and Jeremić, Nevena and Jakovljević, Vladimir and Živković, Vladimir and Bradić, Jovana and Pecarski, Danijela and Tel-Çayan, Gülsen and Glamočlija, Jasmina and Soković, Marina and Gregori, Andrej and Petrović, Jovana",
year = "2023",
abstract = "The present study aimed to examine the biological activity and cardioprotective potential of Trametes versicolor heteropolysaccharides (TVH) in a rat model of metabolic syndrome (MetS). This study included 40 Wistar rats divided into 5 groups: CTRL—healthy non-treated rats; MetS—non-treated rats; and H-TV, M-TV and L-TV-rats with MetS treated with either 300, 200 or 100 mg/kg TVH per os for 4 weeks. After finishing the treatment, we conducted an oral glucose tolerance test (OGTT), hemodynamic measurements and the animals were sacrificed, hearts isolated and subjected to the Langendorff technique. Blood samples were used for the determination of oxidative stress parameters, lipid status and insulin levels. We showed that α-amylase inhibition was not the mode of TVH antidiabetic action, while TVH showed a moderate inhibition of pathogenic microorganisms’ growth (MIC 8.00 mg·mL−1; MBC/MFC 16.00 mg·mL−1). H-TV and M-TV significantly reduced the level of prooxidants (O2−, H2O2, TBARS; p < 0.05), increased antioxidants activity (SOD, CAT, GSH; p < 0.05), reduced blood pressure (p < 0.05), improved glucose homeostasis in the OGTT test (p < 0.05), and ejection fraction (p < 0.05) and cardiac contractility (p < 0.05) compared to MetS (p < 0.05). Moreover, TVH treatment normalized the lipid status and decreased insulin levels compared to MetS rats (p < 0.05). The obtained results demonstrated that the TVH may be considered a useful agent for cardioprotection in MetS conditions.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome",
number = "6",
volume = "16",
doi = "10.3390/ph16060787",
pages = "787"
}

Radovanović, M., Lazarević, N., Novaković, J., Jeremić, N., Jakovljević, V., Živković, V., Bradić, J., Pecarski, D., Tel-Çayan, G., Glamočlija, J., Soković, M., Gregori, A.,& Petrović, J.. (2023). Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome. in Pharmaceuticals
Basel: MDPI., 16(6), 787.
https://doi.org/10.3390/ph16060787

Radovanović M, Lazarević N, Novaković J, Jeremić N, Jakovljević V, Živković V, Bradić J, Pecarski D, Tel-Çayan G, Glamočlija J, Soković M, Gregori A, Petrović J. Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome. in Pharmaceuticals. 2023;16(6):787.
doi:10.3390/ph16060787 .

Radovanović, Marina, Lazarević, Nevena, Novaković, Jovana, Jeremić, Nevena, Jakovljević, Vladimir, Živković, Vladimir, Bradić, Jovana, Pecarski, Danijela, Tel-Çayan, Gülsen, Glamočlija, Jasmina, Soković, Marina, Gregori, Andrej, Petrović, Jovana, "Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome" in Pharmaceuticals, 16, no. 6 (2023):787,
https://doi.org/10.3390/ph16060787 . .

TY  - JOUR
AU  - Joseph, Blessy
AU  - John, Amrit Jose
AU  - Glamočlija, Jasmina
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Lazović, Saša
AU  - Kochupurackal, Jayachandran
AU  - Kalarikkal, Nandakumar
AU  - Thomas, Sabu
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2352492822018025
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5348
AB  - Electrospun fibers with large surface area are of particular importance in the tissue engineering field as their highly porous structure favours cell adhesion, proliferation, and migration. Herein, we report a facile approach for the development of polycaprolactone (PCL) scaffolds incorporated with zirconium dioxide (ZrO2) nanoparticles. Zirconium dioxide nanoparticles were incorporated into the fibers at five different concentrations (0.5, 1, 3, 5 and 7 wt%), based on the amount of polymer. The morphology of the scaffolds was evaluated by scanning electron microscopy which showed interconnected pore structure. The incorporation of nanoparticles was confirmed from EDX studies. The functional groups were analysed using FTIR and crystallinity was analysed by XRD. Mechanical studies demonstrated that the scaffolds containing 1 wt% Zirconium dioxide nanoparticles exhibited the highest tensile strength. The scaffolds showed antibacterial activity against Gram positive bacterium Staphylococcus lugdunensis and were not active against Gram-negative bacterium Proteus vulgaris, at all time-points. ZrO2 loaded PCL scaffolds with good mechanical and biological properties will open avenues for the potential applications of the electrospun membranes for tissue engineering.
PB  - Elsevier Ltd
T2  - Materials Today Communications
T1  - Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds
VL  - 34
DO  - 10.1016/j.mtcomm.2022.104961
SP  - 104961
ER  - 

@article{
author = "Joseph, Blessy and John, Amrit Jose and Glamočlija, Jasmina and Stojković, Dejan and Soković, Marina and Lazović, Saša and Kochupurackal, Jayachandran and Kalarikkal, Nandakumar and Thomas, Sabu",
year = "2023",
abstract = "Electrospun fibers with large surface area are of particular importance in the tissue engineering field as their highly porous structure favours cell adhesion, proliferation, and migration. Herein, we report a facile approach for the development of polycaprolactone (PCL) scaffolds incorporated with zirconium dioxide (ZrO2) nanoparticles. Zirconium dioxide nanoparticles were incorporated into the fibers at five different concentrations (0.5, 1, 3, 5 and 7 wt%), based on the amount of polymer. The morphology of the scaffolds was evaluated by scanning electron microscopy which showed interconnected pore structure. The incorporation of nanoparticles was confirmed from EDX studies. The functional groups were analysed using FTIR and crystallinity was analysed by XRD. Mechanical studies demonstrated that the scaffolds containing 1 wt% Zirconium dioxide nanoparticles exhibited the highest tensile strength. The scaffolds showed antibacterial activity against Gram positive bacterium Staphylococcus lugdunensis and were not active against Gram-negative bacterium Proteus vulgaris, at all time-points. ZrO2 loaded PCL scaffolds with good mechanical and biological properties will open avenues for the potential applications of the electrospun membranes for tissue engineering.",
publisher = "Elsevier Ltd",
journal = "Materials Today Communications",
title = "Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds",
volume = "34",
doi = "10.1016/j.mtcomm.2022.104961",
pages = "104961"
}

Joseph, B., John, A. J., Glamočlija, J., Stojković, D., Soković, M., Lazović, S., Kochupurackal, J., Kalarikkal, N.,& Thomas, S.. (2023). Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds. in Materials Today Communications
Elsevier Ltd., 34, 104961.
https://doi.org/10.1016/j.mtcomm.2022.104961

Joseph B, John AJ, Glamočlija J, Stojković D, Soković M, Lazović S, Kochupurackal J, Kalarikkal N, Thomas S. Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds. in Materials Today Communications. 2023;34:104961.
doi:10.1016/j.mtcomm.2022.104961 .

Joseph, Blessy, John, Amrit Jose, Glamočlija, Jasmina, Stojković, Dejan, Soković, Marina, Lazović, Saša, Kochupurackal, Jayachandran, Kalarikkal, Nandakumar, Thomas, Sabu, "Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds" in Materials Today Communications, 34 (2023):104961,
https://doi.org/10.1016/j.mtcomm.2022.104961 . .

TY  - JOUR
AU  - Ak, Gunes
AU  - Zengin, Gokhan
AU  - Dall’Acqua, Stefano
AU  - Ferrarese, Irene
AU  - Sut, Stefania
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Nenadić, Marija
AU  - Chiavaroli, Annalisa
AU  - Recinella, Lucia
AU  - Leone, Sheila
AU  - Orlando, Giustino
AU  - Menghini, Luigi
AU  - Ferrante, Claudio
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5069
AB  - The genus Scorzonera is one of the most important genera in the Asteraceae family, and members of the genus have great potential in traditional medicine systems. In this article we aimed to determine skin protective and antimicrobial effects of different extracts from the roots and aerial parts of three Scorzonera species (S. hieraciifolia, S. hispanica, S. tomentosa). Antimicrobial properties were evaluated against different pathogen bacterial and fungal strains, whereas protective effects were studied in isolated mouse skin specimens exposed to hydrogen peroxide. In this biological model, the levels of l-dopa and prostaglandin E2 (PGE2) were evaluated, as markers of tyrosinase activity and inflammation, respectively. All extracts were effective in blunting the increased hydrogen peroxide-induced l-dopa turnover and PGE2 level; thus, suggesting skin protection that could be partly related to the content of flavonoids, among which rutin, in the tested extracts. Additionally, all extracts were effective as antibacterial and antifungal agents; intriguing results were observed against Trichoderma viride, which was particularly sensitive to S. tomentosa root extracts. Therefore, the present study strengthens the importance of investigating herbal preparations obtained from plants belonging to the genus Scorzonera, for the management and prevention of inflammatory and infectious skin conditions.
PB  - Taylor and Francis Ltd.
T2  - Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology
T1  - A new step on the chemical profiles and pharmacological effects of three Scorzonera species ( S. hieraciifolia , S. hispanica and S. tomentosa )
IS  - 1
VL  - 157
DO  - 10.1080/11263504.2022.2089766
SP  - 119
EP  - 128
ER  - 

@article{
author = "Ak, Gunes and Zengin, Gokhan and Dall’Acqua, Stefano and Ferrarese, Irene and Sut, Stefania and Glamočlija, Jasmina and Soković, Marina and Nenadić, Marija and Chiavaroli, Annalisa and Recinella, Lucia and Leone, Sheila and Orlando, Giustino and Menghini, Luigi and Ferrante, Claudio",
year = "2023",
abstract = "The genus Scorzonera is one of the most important genera in the Asteraceae family, and members of the genus have great potential in traditional medicine systems. In this article we aimed to determine skin protective and antimicrobial effects of different extracts from the roots and aerial parts of three Scorzonera species (S. hieraciifolia, S. hispanica, S. tomentosa). Antimicrobial properties were evaluated against different pathogen bacterial and fungal strains, whereas protective effects were studied in isolated mouse skin specimens exposed to hydrogen peroxide. In this biological model, the levels of l-dopa and prostaglandin E2 (PGE2) were evaluated, as markers of tyrosinase activity and inflammation, respectively. All extracts were effective in blunting the increased hydrogen peroxide-induced l-dopa turnover and PGE2 level; thus, suggesting skin protection that could be partly related to the content of flavonoids, among which rutin, in the tested extracts. Additionally, all extracts were effective as antibacterial and antifungal agents; intriguing results were observed against Trichoderma viride, which was particularly sensitive to S. tomentosa root extracts. Therefore, the present study strengthens the importance of investigating herbal preparations obtained from plants belonging to the genus Scorzonera, for the management and prevention of inflammatory and infectious skin conditions.",
publisher = "Taylor and Francis Ltd.",
journal = "Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology",
title = "A new step on the chemical profiles and pharmacological effects of three Scorzonera species ( S. hieraciifolia , S. hispanica and S. tomentosa )",
number = "1",
volume = "157",
doi = "10.1080/11263504.2022.2089766",
pages = "119-128"
}

Ak, G., Zengin, G., Dall’Acqua, S., Ferrarese, I., Sut, S., Glamočlija, J., Soković, M., Nenadić, M., Chiavaroli, A., Recinella, L., Leone, S., Orlando, G., Menghini, L.,& Ferrante, C.. (2023). A new step on the chemical profiles and pharmacological effects of three Scorzonera species ( S. hieraciifolia , S. hispanica and S. tomentosa ). in Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology
Taylor and Francis Ltd.., 157(1), 119-128.
https://doi.org/10.1080/11263504.2022.2089766

Ak G, Zengin G, Dall’Acqua S, Ferrarese I, Sut S, Glamočlija J, Soković M, Nenadić M, Chiavaroli A, Recinella L, Leone S, Orlando G, Menghini L, Ferrante C. A new step on the chemical profiles and pharmacological effects of three Scorzonera species ( S. hieraciifolia , S. hispanica and S. tomentosa ). in Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 2023;157(1):119-128.
doi:10.1080/11263504.2022.2089766 .

Ak, Gunes, Zengin, Gokhan, Dall’Acqua, Stefano, Ferrarese, Irene, Sut, Stefania, Glamočlija, Jasmina, Soković, Marina, Nenadić, Marija, Chiavaroli, Annalisa, Recinella, Lucia, Leone, Sheila, Orlando, Giustino, Menghini, Luigi, Ferrante, Claudio, "A new step on the chemical profiles and pharmacological effects of three Scorzonera species ( S. hieraciifolia , S. hispanica and S. tomentosa )" in Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 157, no. 1 (2023):119-128,
https://doi.org/10.1080/11263504.2022.2089766 . .

TY  - JOUR
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Glamočlija, Jasmina
AU  - Soković Bajić, Svetlana
AU  - Tolinacki, Maja
AU  - Golić, Nataša
AU  - Mirkov, Ivana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6432
AB  - Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigatedbacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiotacomposition) in a healthy state and during pulmonary infection caused by the opportunistic fungusAspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DAand AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterizedby increased alpha diversity indices and unchanged beta diversity. The relative abundance ofgenera and species was increased in DA but decreased in AO rats during infection. Changes in lungmicrobiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparateresponse of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two ratstrains differentially susceptible to a subsequent inflammatory insult.
PB  - Paris: Elsevier Masson SAS
T2  - Microbes and Infection
T1  - Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats
DO  - 10.1016/j.micinf.2023.105186
SP  - 105186
ER  - 

@article{
author = "Popović, Dušanka and Kulaš, Jelena and Tucović, Dina and Popov Aleksandrov, Aleksandra and Glamočlija, Jasmina and Soković Bajić, Svetlana and Tolinacki, Maja and Golić, Nataša and Mirkov, Ivana",
year = "2023",
abstract = "Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigatedbacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiotacomposition) in a healthy state and during pulmonary infection caused by the opportunistic fungusAspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DAand AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterizedby increased alpha diversity indices and unchanged beta diversity. The relative abundance ofgenera and species was increased in DA but decreased in AO rats during infection. Changes in lungmicrobiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparateresponse of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two ratstrains differentially susceptible to a subsequent inflammatory insult.",
publisher = "Paris: Elsevier Masson SAS",
journal = "Microbes and Infection",
title = "Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats",
doi = "10.1016/j.micinf.2023.105186",
pages = "105186"
}

Popović, D., Kulaš, J., Tucović, D., Popov Aleksandrov, A., Glamočlija, J., Soković Bajić, S., Tolinacki, M., Golić, N.,& Mirkov, I.. (2023). Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. in Microbes and Infection
Paris: Elsevier Masson SAS., 105186.
https://doi.org/10.1016/j.micinf.2023.105186

Popović D, Kulaš J, Tucović D, Popov Aleksandrov A, Glamočlija J, Soković Bajić S, Tolinacki M, Golić N, Mirkov I. Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. in Microbes and Infection. 2023;:105186.
doi:10.1016/j.micinf.2023.105186 .

Popović, Dušanka, Kulaš, Jelena, Tucović, Dina, Popov Aleksandrov, Aleksandra, Glamočlija, Jasmina, Soković Bajić, Svetlana, Tolinacki, Maja, Golić, Nataša, Mirkov, Ivana, "Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats" in Microbes and Infection (2023):105186,
https://doi.org/10.1016/j.micinf.2023.105186 . .

TY  - JOUR
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Glamočlija, Jasmina
AU  - Soković Bajić, Svetlana
AU  - Tolinacki, Maja
AU  - Golić, Nataša
AU  - Mirkov, Ivana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6162
AB  - Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigated
bacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiota
composition) in a healthy state and during pulmonary infection caused by the opportunistic fungus
Aspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DA
and AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterized
by increased alpha diversity indices and unchanged beta diversity. The relative abundance of
genera and species was increased in DA but decreased in AO rats during infection. Changes in lung
microbiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparate
response of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two rat
strains differentially susceptible to a subsequent inflammatory insult.
PB  - Paris: Elsevier Masson SAS
T2  - Microbes and Infection
T1  - Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats
DO  - 10.1016/j.micinf.2023.105186
SP  - 105186
ER  - 

@article{
author = "Popović, Dušanka and Kulaš, Jelena and Tucović, Dina and Popov Aleksandrov, Aleksandra and Glamočlija, Jasmina and Soković Bajić, Svetlana and Tolinacki, Maja and Golić, Nataša and Mirkov, Ivana",
year = "2023",
abstract = "Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigated
bacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiota
composition) in a healthy state and during pulmonary infection caused by the opportunistic fungus
Aspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DA
and AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterized
by increased alpha diversity indices and unchanged beta diversity. The relative abundance of
genera and species was increased in DA but decreased in AO rats during infection. Changes in lung
microbiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparate
response of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two rat
strains differentially susceptible to a subsequent inflammatory insult.",
publisher = "Paris: Elsevier Masson SAS",
journal = "Microbes and Infection",
title = "Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats",
doi = "10.1016/j.micinf.2023.105186",
pages = "105186"
}

Popović, D., Kulaš, J., Tucović, D., Popov Aleksandrov, A., Glamočlija, J., Soković Bajić, S., Tolinacki, M., Golić, N.,& Mirkov, I.. (2023). Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. in Microbes and Infection
Paris: Elsevier Masson SAS., 105186.
https://doi.org/10.1016/j.micinf.2023.105186

Popović D, Kulaš J, Tucović D, Popov Aleksandrov A, Glamočlija J, Soković Bajić S, Tolinacki M, Golić N, Mirkov I. Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. in Microbes and Infection. 2023;:105186.
doi:10.1016/j.micinf.2023.105186 .

Popović, Dušanka, Kulaš, Jelena, Tucović, Dina, Popov Aleksandrov, Aleksandra, Glamočlija, Jasmina, Soković Bajić, Svetlana, Tolinacki, Maja, Golić, Nataša, Mirkov, Ivana, "Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats" in Microbes and Infection (2023):105186,
https://doi.org/10.1016/j.micinf.2023.105186 . .

TY  - CONF
AU  - Glamočlija, Jasmina
AU  - Stanojković, Jelena
AU  - Janošević, Dušica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6370
AB  - The oldest data on hypogeous fungi from the territory of Serbia date
back to the year of 1777, but first study on truffles and truffle-like fungi in Serbia
started in 1992. Collected material, fruit bodies of Tuber species had been
founded and identified and extensively studied during the last decade of the
twentieth century.
Despite the current interest in truffles in Serbia, there is not much
information about artificially established truffle plantations. The register
orchards of truffles in Serbia have not been created yet.
Thus, the objective of this study was the morphological and anatomical
characterization of an unknown ectomycorrhizas of host species Populus alba
from cultivated seedlings from part of a ten-year-old truffles plantation.
In autumn 2016, we were invited to visit and determine what kind of
mycorrhizae is situated in the plantation near the town of Požarevac (Eastern
Serbia). The truffle-ground (about 3,000 m2) is placed in Kličevac where a
plantation was established during the autumn 2007. According to the owner, he
bought commercially produced seedlings of Populus alba inoculated with the
black truffle.
PB  - Belgrade: Institute for Multidisciplinary Research, University of Belgrade
C3  - Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia
T1  - Characterization of Tuber macrosporum / Populus alba mycorrhizas from cultivated seedlings: case report
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6370
ER  - 

@conference{
author = "Glamočlija, Jasmina and Stanojković, Jelena and Janošević, Dušica",
year = "2023",
abstract = "The oldest data on hypogeous fungi from the territory of Serbia date
back to the year of 1777, but first study on truffles and truffle-like fungi in Serbia
started in 1992. Collected material, fruit bodies of Tuber species had been
founded and identified and extensively studied during the last decade of the
twentieth century.
Despite the current interest in truffles in Serbia, there is not much
information about artificially established truffle plantations. The register
orchards of truffles in Serbia have not been created yet.
Thus, the objective of this study was the morphological and anatomical
characterization of an unknown ectomycorrhizas of host species Populus alba
from cultivated seedlings from part of a ten-year-old truffles plantation.
In autumn 2016, we were invited to visit and determine what kind of
mycorrhizae is situated in the plantation near the town of Požarevac (Eastern
Serbia). The truffle-ground (about 3,000 m2) is placed in Kličevac where a
plantation was established during the autumn 2007. According to the owner, he
bought commercially produced seedlings of Populus alba inoculated with the
black truffle.",
publisher = "Belgrade: Institute for Multidisciplinary Research, University of Belgrade",
journal = "Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia",
title = "Characterization of Tuber macrosporum / Populus alba mycorrhizas from cultivated seedlings: case report",
pages = "37",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6370"
}

Glamočlija, J., Stanojković, J.,& Janošević, D.. (2023). Characterization of Tuber macrosporum / Populus alba mycorrhizas from cultivated seedlings: case report. in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia
Belgrade: Institute for Multidisciplinary Research, University of Belgrade., 37.
https://hdl.handle.net/21.15107/rcub_ibiss_6370

Glamočlija J, Stanojković J, Janošević D. Characterization of Tuber macrosporum / Populus alba mycorrhizas from cultivated seedlings: case report. in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia. 2023;:37.
https://hdl.handle.net/21.15107/rcub_ibiss_6370 .

Glamočlija, Jasmina, Stanojković, Jelena, Janošević, Dušica, "Characterization of Tuber macrosporum / Populus alba mycorrhizas from cultivated seedlings: case report" in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia (2023):37,
https://hdl.handle.net/21.15107/rcub_ibiss_6370 .

TY  - GEN
AU  - Marjanović, Žaklina
AU  - Glišić, Aleksa
AU  - Stefanović, Slobodan
AU  - Glamočlija, Jasmina
AU  - Janošević, Dušica
AU  - Saljnikov, Elmira
AU  - Leonardi, Marco
AU  - Paccioni, Giovanni
AU  - Iotti, Mirco
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6203
AB  - Truffle diversity of Balkan Peninsula does not stop to surprise the
scientific community with three new species from the melanosporum clade
described in last year's (Mereny et al 2017, Milenković at al 2015, Slavova et al
2023). Out of large set of ITS sequences, Mereny at al. (2017) described T.
cryptobrumale as connected to Pannonian region, but two other genetically quite
distinctive populations were not distinguished as species. In this contribution, we
have analyzed larger set of sequences and their habitats and detected strong
connection between specimens with specific ITS/LSU region variants to
ecologically specific habitats of Balkan peninsula. Soils and vegetation where
these specimens have been detected rather resemble descriptions of T.
melanosporum sites in Italy, while habitats of T. cryptobrumale resemble those of
T. magnatum. Additionally, we have detected numerous populations of T.
melanosporum in the coastal regions of Croatia, while this species has never been
officially detected eastern from Dinaric alps in Balkan peninsula. We hypothesize
that T. melanosporum could never overcome low temperatures of high and long
Dinaric massive and is therefore localized in the western Mediterranean regions.
This could be the reason why the melanosporum clade has been highly
diversifying in Balkan regions. Moreover, the genetic variant widely distributed
all over Europe and described as “T. brumale” has never been detected in the
Balkans. Therefore, we propose that ecologically and genetically specific taxon
that is widespread only in Balkan peninsula (and to some extent northwards)
should be recognized as a different species - Tuber balkanicum.
PB  - Belgrade: Institute for Multidisciplinary Research, University of Belgrade
T2  - Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia
T1  - Tuber balcanicum – new taxon defined by molecular, ecological and biogeographical methods
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6203
ER  - 

@misc{
author = "Marjanović, Žaklina and Glišić, Aleksa and Stefanović, Slobodan and Glamočlija, Jasmina and Janošević, Dušica and Saljnikov, Elmira and Leonardi, Marco and Paccioni, Giovanni and Iotti, Mirco",
year = "2023",
abstract = "Truffle diversity of Balkan Peninsula does not stop to surprise the
scientific community with three new species from the melanosporum clade
described in last year's (Mereny et al 2017, Milenković at al 2015, Slavova et al
2023). Out of large set of ITS sequences, Mereny at al. (2017) described T.
cryptobrumale as connected to Pannonian region, but two other genetically quite
distinctive populations were not distinguished as species. In this contribution, we
have analyzed larger set of sequences and their habitats and detected strong
connection between specimens with specific ITS/LSU region variants to
ecologically specific habitats of Balkan peninsula. Soils and vegetation where
these specimens have been detected rather resemble descriptions of T.
melanosporum sites in Italy, while habitats of T. cryptobrumale resemble those of
T. magnatum. Additionally, we have detected numerous populations of T.
melanosporum in the coastal regions of Croatia, while this species has never been
officially detected eastern from Dinaric alps in Balkan peninsula. We hypothesize
that T. melanosporum could never overcome low temperatures of high and long
Dinaric massive and is therefore localized in the western Mediterranean regions.
This could be the reason why the melanosporum clade has been highly
diversifying in Balkan regions. Moreover, the genetic variant widely distributed
all over Europe and described as “T. brumale” has never been detected in the
Balkans. Therefore, we propose that ecologically and genetically specific taxon
that is widespread only in Balkan peninsula (and to some extent northwards)
should be recognized as a different species - Tuber balkanicum.",
publisher = "Belgrade: Institute for Multidisciplinary Research, University of Belgrade",
journal = "Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia",
title = "Tuber balcanicum – new taxon defined by molecular, ecological and biogeographical methods",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6203"
}

Marjanović, Ž., Glišić, A., Stefanović, S., Glamočlija, J., Janošević, D., Saljnikov, E., Leonardi, M., Paccioni, G.,& Iotti, M.. (2023). Tuber balcanicum – new taxon defined by molecular, ecological and biogeographical methods. in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia
Belgrade: Institute for Multidisciplinary Research, University of Belgrade., 19.
https://hdl.handle.net/21.15107/rcub_ibiss_6203

Marjanović Ž, Glišić A, Stefanović S, Glamočlija J, Janošević D, Saljnikov E, Leonardi M, Paccioni G, Iotti M. Tuber balcanicum – new taxon defined by molecular, ecological and biogeographical methods. in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia. 2023;:19.
https://hdl.handle.net/21.15107/rcub_ibiss_6203 .

Marjanović, Žaklina, Glišić, Aleksa, Stefanović, Slobodan, Glamočlija, Jasmina, Janošević, Dušica, Saljnikov, Elmira, Leonardi, Marco, Paccioni, Giovanni, Iotti, Mirco, "Tuber balcanicum – new taxon defined by molecular, ecological and biogeographical methods" in Book of abstracts: Truffle Research Union of Europe; 2023 Sep 27-30, Belgrade, Serbia (2023):19,
https://hdl.handle.net/21.15107/rcub_ibiss_6203 .

TY  - CONF
AU  - Popović, Dušanka
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Malešević, Anastasija
AU  - Glamočlija, Jasmina
AU  - Soković Bajić, Svetlana
AU  - Brdarić, Emilija
AU  - Tolinački, Maja
AU  - Golić, Nataša
PY  - 2023
UR  - https://probiotics-prebiotics-newfood.com/wp-content/uploads/2023/09/Pogramme-web-07_09_2023.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6166
AB  - Objective:
The little data of the effect of pulmonary fungal infection on lung and
gut microbiota exist, thus aim of our study was to examine the effect of
Aspergillus fumigatus infection on bacterial homestasis in these organs in
Dark Agouti (DA) and Albino Oxford (AO) rats known to develop different
immune responce to the fungus.
Methods:
16S rRNA amplicon sequencing of the V3-V4 hypervariable region.
Enzyme-linked immunosorbent assay for cytokine content (IL-10, IL-6, TNF,
IL-1beta, IL-17 i IFN-gamma) in lung and gut homogenats, and plasma (IL-
6, IL-17, TNF). Myeloperoxidase, catalase, glutathione S-transferase and
content of reduced glutathione in lung homogenates.
Results:
No initial differences in lung bacterial microbiota between healthy DA
and AO rats exists, but A. fumigatus infection resulted in increase in
alpha diversity (without effect on bacterial composition) in both strains.
Changes in relative abundance at genus and species level were expressed
differently (increased in DA, decreased in AO). Dysbiosis in lung coincided
with increase of IFN-gamma, IL-17 and MPO in both strains, and increase
oxidative stress solely in DA. Gut inflammation was noted in both strains,
while bacterial dysbiosis only in DA.
Conclusions:
Pulmonary fungal infection resulted in bacterial dysbiosis in lung (in both
rat strains), and gut (solely in DA). Noted lung and gut inflammation in
these two rat strains, coexisted with different effects on bacterial
homeostasis, which could be due to different genetic background or
bacterial composition prior infection.
PB  - Roma: EMEC
C3  - 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy
T1  - The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats
SP  - 90
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6166
ER  - 

@conference{
author = "Popović, Dušanka and Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Tucović, Dina and Kulaš, Jelena and Malešević, Anastasija and Glamočlija, Jasmina and Soković Bajić, Svetlana and Brdarić, Emilija and Tolinački, Maja and Golić, Nataša",
year = "2023",
abstract = "Objective:
The little data of the effect of pulmonary fungal infection on lung and
gut microbiota exist, thus aim of our study was to examine the effect of
Aspergillus fumigatus infection on bacterial homestasis in these organs in
Dark Agouti (DA) and Albino Oxford (AO) rats known to develop different
immune responce to the fungus.
Methods:
16S rRNA amplicon sequencing of the V3-V4 hypervariable region.
Enzyme-linked immunosorbent assay for cytokine content (IL-10, IL-6, TNF,
IL-1beta, IL-17 i IFN-gamma) in lung and gut homogenats, and plasma (IL-
6, IL-17, TNF). Myeloperoxidase, catalase, glutathione S-transferase and
content of reduced glutathione in lung homogenates.
Results:
No initial differences in lung bacterial microbiota between healthy DA
and AO rats exists, but A. fumigatus infection resulted in increase in
alpha diversity (without effect on bacterial composition) in both strains.
Changes in relative abundance at genus and species level were expressed
differently (increased in DA, decreased in AO). Dysbiosis in lung coincided
with increase of IFN-gamma, IL-17 and MPO in both strains, and increase
oxidative stress solely in DA. Gut inflammation was noted in both strains,
while bacterial dysbiosis only in DA.
Conclusions:
Pulmonary fungal infection resulted in bacterial dysbiosis in lung (in both
rat strains), and gut (solely in DA). Noted lung and gut inflammation in
these two rat strains, coexisted with different effects on bacterial
homeostasis, which could be due to different genetic background or
bacterial composition prior infection.",
publisher = "Roma: EMEC",
journal = "12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy",
title = "The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats",
pages = "90-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6166"
}

Popović, D., Mirkov, I., Popov Aleksandrov, A., Tucović, D., Kulaš, J., Malešević, A., Glamočlija, J., Soković Bajić, S., Brdarić, E., Tolinački, M.,& Golić, N.. (2023). The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats. in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy
Roma: EMEC., 90-90.
https://hdl.handle.net/21.15107/rcub_ibiss_6166

Popović D, Mirkov I, Popov Aleksandrov A, Tucović D, Kulaš J, Malešević A, Glamočlija J, Soković Bajić S, Brdarić E, Tolinački M, Golić N. The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats. in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy. 2023;:90-90.
https://hdl.handle.net/21.15107/rcub_ibiss_6166 .

Popović, Dušanka, Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Tucović, Dina, Kulaš, Jelena, Malešević, Anastasija, Glamočlija, Jasmina, Soković Bajić, Svetlana, Brdarić, Emilija, Tolinački, Maja, Golić, Nataša, "The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats" in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy (2023):90-90,
https://hdl.handle.net/21.15107/rcub_ibiss_6166 .

TY  - JOUR
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Malešević, Anastasija
AU  - Glamočlija, Jasmina
AU  - Brdarić, Emilija
AU  - Soković Bajić, Svetlana
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Tolinački, Maja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6084
AB  - While the effect of gut microbiota and/or inflammation on a distant body site, including the lungs (gut–lung axis), has been well characterized, data about the influence of lung microbiota and lung inflammation on gut homeostasis (lung–gut axis) are scarce. Using a well-characterized model of pulmonary infection with the fungus Aspergillus fumigatus, we investigated alterations in the lung and gut microbiota by next-generation sequencing of the V3–V4 regions of total bacterial DNA. Pulmonary inflammation due to the fungus A. fumigatus caused bacterial dysbiosis in both lungs and gut, but with different characteristics. While increased alpha diversity and unchanged bacterial composition were noted in the lungs, dysbiosis in the gut was characterized by decreased alpha diversity indices and modified bacterial composition. The altered homeostasis in the lungs allows the immigration of new bacterial species of which 41.8% were found in the feces, indicating that some degree of bacterial migration from the gut to the lungs occurs. On the contrary, the dysbiosis occurring in the gut during pulmonary infection was a consequence of the local activity of the immune system. In addition, the alteration of gut microbiota in response to pulmonary infection depends on the bacterial composition before infection, as no changes in gut bacterial microbiota were detected in a rat strain with diverse gut bacteria. The data presented support the existence of the lung–gut axis and provide additional insight into this mechanism.
PB  - American Society for Microbiology
T2  - Microbiology Spectrum
T1  - Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition
VL  - 49
DO  - 10.1128/spectrum.01990-23
SP  - e0199023
ER  - 

@article{
author = "Popović, Dušanka and Kulaš, Jelena and Tucović, Dina and Popov Aleksandrov, Aleksandra and Malešević, Anastasija and Glamočlija, Jasmina and Brdarić, Emilija and Soković Bajić, Svetlana and Golić, Nataša and Mirkov, Ivana and Tolinački, Maja",
year = "2023",
abstract = "While the effect of gut microbiota and/or inflammation on a distant body site, including the lungs (gut–lung axis), has been well characterized, data about the influence of lung microbiota and lung inflammation on gut homeostasis (lung–gut axis) are scarce. Using a well-characterized model of pulmonary infection with the fungus Aspergillus fumigatus, we investigated alterations in the lung and gut microbiota by next-generation sequencing of the V3–V4 regions of total bacterial DNA. Pulmonary inflammation due to the fungus A. fumigatus caused bacterial dysbiosis in both lungs and gut, but with different characteristics. While increased alpha diversity and unchanged bacterial composition were noted in the lungs, dysbiosis in the gut was characterized by decreased alpha diversity indices and modified bacterial composition. The altered homeostasis in the lungs allows the immigration of new bacterial species of which 41.8% were found in the feces, indicating that some degree of bacterial migration from the gut to the lungs occurs. On the contrary, the dysbiosis occurring in the gut during pulmonary infection was a consequence of the local activity of the immune system. In addition, the alteration of gut microbiota in response to pulmonary infection depends on the bacterial composition before infection, as no changes in gut bacterial microbiota were detected in a rat strain with diverse gut bacteria. The data presented support the existence of the lung–gut axis and provide additional insight into this mechanism.",
publisher = "American Society for Microbiology",
journal = "Microbiology Spectrum",
title = "Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition",
volume = "49",
doi = "10.1128/spectrum.01990-23",
pages = "e0199023"
}

Popović, D., Kulaš, J., Tucović, D., Popov Aleksandrov, A., Malešević, A., Glamočlija, J., Brdarić, E., Soković Bajić, S., Golić, N., Mirkov, I.,& Tolinački, M.. (2023). Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition. in Microbiology Spectrum
American Society for Microbiology., 49, e0199023.
https://doi.org/10.1128/spectrum.01990-23

Popović D, Kulaš J, Tucović D, Popov Aleksandrov A, Malešević A, Glamočlija J, Brdarić E, Soković Bajić S, Golić N, Mirkov I, Tolinački M. Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition. in Microbiology Spectrum. 2023;49:e0199023.
doi:10.1128/spectrum.01990-23 .

Popović, Dušanka, Kulaš, Jelena, Tucović, Dina, Popov Aleksandrov, Aleksandra, Malešević, Anastasija, Glamočlija, Jasmina, Brdarić, Emilija, Soković Bajić, Svetlana, Golić, Nataša, Mirkov, Ivana, Tolinački, Maja, "Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition" in Microbiology Spectrum, 49 (2023):e0199023,
https://doi.org/10.1128/spectrum.01990-23 . .

TY  - JOUR
AU  - Perić, Mirjana
AU  - Čairović, Aleksandra
AU  - Špadijer Gostović, Aleksandra
AU  - Stanimirović, Dragan
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Pavlović, Vladimir B.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6005
AB  - The purpose of this study was to evaluate the surface texture and biofilm adhe sion of veneered or CAD/CAM milled zirconia (partially stabilized with yttrium) after professional
oral hygiene procedures. The samples (4 × 4 mm, thickness 2 mm; n = 72) were separated from
zirconia blanks (3Y-TZP-LA). One group was veenered with ceramics, and the other group of samples
was CAD/CAM milled. Each group had two subgroups: polished and glazed. The samples were
subjected to simulated strokes of professional brushing using abrasive paste and ultrasonic scaling.
The parameters of surface micromorphology and receptivity to biofilm were calculated before and
after simulating the given methods of the professional maintenance of oral hygiene. Scanning electron
microscopy (SEM) was used to evaluate zirconia surface properties. Microbial (bacterial/fungal)
species (Staphylococcus aureus, Streptococcus sanguinis and Candida albicans) were used and cultured
on respective sterilized zirconia surfaces. Colony-forming unit (CFU) counts were used to quantify
the amount of biofilm formation on zirconia samples surfaces. Results: The SEM analysis showed
the greatest change in surface microtopography after the use of ultrasonic scaling on glazed zirconia
samples. Less formation of colonies on the surfaces of CAD/CAM milled zirconia restorations was
observed. Conclusion: Routine methods of oral hygiene professional maintenance can damage the
surfaces of glazed zirconia restorations.
PB  - Basel: MDPI
T2  - Applied Sciences
T1  - Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study
IS  - 14
VL  - 13
DO  - 10.3390/app13148389
SP  - 8389
ER  - 

@article{
author = "Perić, Mirjana and Čairović, Aleksandra and Špadijer Gostović, Aleksandra and Stanimirović, Dragan and Kostić, Marina and Ivanov, Marija and Ćirić, Ana and Glamočlija, Jasmina and Pavlović, Vladimir B.",
year = "2023",
abstract = "The purpose of this study was to evaluate the surface texture and biofilm adhe sion of veneered or CAD/CAM milled zirconia (partially stabilized with yttrium) after professional
oral hygiene procedures. The samples (4 × 4 mm, thickness 2 mm; n = 72) were separated from
zirconia blanks (3Y-TZP-LA). One group was veenered with ceramics, and the other group of samples
was CAD/CAM milled. Each group had two subgroups: polished and glazed. The samples were
subjected to simulated strokes of professional brushing using abrasive paste and ultrasonic scaling.
The parameters of surface micromorphology and receptivity to biofilm were calculated before and
after simulating the given methods of the professional maintenance of oral hygiene. Scanning electron
microscopy (SEM) was used to evaluate zirconia surface properties. Microbial (bacterial/fungal)
species (Staphylococcus aureus, Streptococcus sanguinis and Candida albicans) were used and cultured
on respective sterilized zirconia surfaces. Colony-forming unit (CFU) counts were used to quantify
the amount of biofilm formation on zirconia samples surfaces. Results: The SEM analysis showed
the greatest change in surface microtopography after the use of ultrasonic scaling on glazed zirconia
samples. Less formation of colonies on the surfaces of CAD/CAM milled zirconia restorations was
observed. Conclusion: Routine methods of oral hygiene professional maintenance can damage the
surfaces of glazed zirconia restorations.",
publisher = "Basel: MDPI",
journal = "Applied Sciences",
title = "Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study",
number = "14",
volume = "13",
doi = "10.3390/app13148389",
pages = "8389"
}

Perić, M., Čairović, A., Špadijer Gostović, A., Stanimirović, D., Kostić, M., Ivanov, M., Ćirić, A., Glamočlija, J.,& Pavlović, V. B.. (2023). Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study. in Applied Sciences
Basel: MDPI., 13(14), 8389.
https://doi.org/10.3390/app13148389

Perić M, Čairović A, Špadijer Gostović A, Stanimirović D, Kostić M, Ivanov M, Ćirić A, Glamočlija J, Pavlović VB. Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study. in Applied Sciences. 2023;13(14):8389.
doi:10.3390/app13148389 .

Perić, Mirjana, Čairović, Aleksandra, Špadijer Gostović, Aleksandra, Stanimirović, Dragan, Kostić, Marina, Ivanov, Marija, Ćirić, Ana, Glamočlija, Jasmina, Pavlović, Vladimir B., "Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study" in Applied Sciences, 13, no. 14 (2023):8389,
https://doi.org/10.3390/app13148389 . .

TY  - JOUR
AU  - Barbakadze, Vakhtang
AU  - Merlani, Maia
AU  - Gogilashvili, Lali
AU  - Amiranashvili, Lela
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2023
UR  - https://www.mdpi.com/2079-6382/12/2/285
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36830198
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9952037
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5491
AB  - This study reports the antimicrobial activities of the biopolymers poly[3-(3,4-dihydoxyphenyl)glyceric acid] (PDHPGA) and poly[2-methoxycarbonyl-3-(3,4-dihydroxyphenyl)oxirane] (PMDHPO), extracted from the six plants of Boraginaceae family: Symphytum asperum (SA), S. caucasicum (SC), S. gr and iflorum (SG), Anchusa italica (AI), Cynoglosum officinale (CO), and Borago officinalis (BO) collected in various parts of Georgia. The study revealed that the antibacterial activities were moderate, and biopolymers from only three plants showed activities against all tested bacteria. Biopolymers from CO stems as well as SC and AI did not show any activity except low activity against a resistant P. aeruginosa strain, which was the most resistant among all three resistant strains. On the other hand, the antifungal activity was better compared to the antibacterial activity. Biopolymers from BO stems exhibited the best activities with MIC/MFC at 0.37-1.00 mg/mL and 0.75-1.5 mg/L, respectively, followed by those from SG stems. Biopolymers from SC and AI roots showed antifungal activities against all six fungi, in contrast to the antibacterial activity, while biopolymers from CO stems and SA roots had activities against four fungi and one fungus, respectively. The sugar-based catechol-containing biopolymers from BO stems demonstrated the best activities among all tested biopolymers against T. viride, P. funiculosum, P. cyclpoium var verucosum, and C. albicans (MIC 0.37 mg/mL). In addition, biopolymers from SG stems were half as active against A. fumigatus and T. viride as ketoconazole. Biopolymers from all plant materials except for CO stems showed higher potency than ketoconazole against T. viride. For the first time, it was shown that all plant materials exhibited better activity against C. albicans, one of the most dreadful fungal species.
PB  - Basel: MDPI
T2  - Antibiotics (Basel, Switzerland)
T1  - Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family
IS  - 2
VL  - 12
DO  - 10.3390/antibiotics12020285
SP  - 285
ER  - 

@article{
author = "Barbakadze, Vakhtang and Merlani, Maia and Gogilashvili, Lali and Amiranashvili, Lela and Petrou, Anthi and Geronikaki, Athina and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2023",
abstract = "This study reports the antimicrobial activities of the biopolymers poly[3-(3,4-dihydoxyphenyl)glyceric acid] (PDHPGA) and poly[2-methoxycarbonyl-3-(3,4-dihydroxyphenyl)oxirane] (PMDHPO), extracted from the six plants of Boraginaceae family: Symphytum asperum (SA), S. caucasicum (SC), S. gr and iflorum (SG), Anchusa italica (AI), Cynoglosum officinale (CO), and Borago officinalis (BO) collected in various parts of Georgia. The study revealed that the antibacterial activities were moderate, and biopolymers from only three plants showed activities against all tested bacteria. Biopolymers from CO stems as well as SC and AI did not show any activity except low activity against a resistant P. aeruginosa strain, which was the most resistant among all three resistant strains. On the other hand, the antifungal activity was better compared to the antibacterial activity. Biopolymers from BO stems exhibited the best activities with MIC/MFC at 0.37-1.00 mg/mL and 0.75-1.5 mg/L, respectively, followed by those from SG stems. Biopolymers from SC and AI roots showed antifungal activities against all six fungi, in contrast to the antibacterial activity, while biopolymers from CO stems and SA roots had activities against four fungi and one fungus, respectively. The sugar-based catechol-containing biopolymers from BO stems demonstrated the best activities among all tested biopolymers against T. viride, P. funiculosum, P. cyclpoium var verucosum, and C. albicans (MIC 0.37 mg/mL). In addition, biopolymers from SG stems were half as active against A. fumigatus and T. viride as ketoconazole. Biopolymers from all plant materials except for CO stems showed higher potency than ketoconazole against T. viride. For the first time, it was shown that all plant materials exhibited better activity against C. albicans, one of the most dreadful fungal species.",
publisher = "Basel: MDPI",
journal = "Antibiotics (Basel, Switzerland)",
title = "Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family",
number = "2",
volume = "12",
doi = "10.3390/antibiotics12020285",
pages = "285"
}

Barbakadze, V., Merlani, M., Gogilashvili, L., Amiranashvili, L., Petrou, A., Geronikaki, A., Ćirić, A., Glamočlija, J.,& Soković, M.. (2023). Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family. in Antibiotics (Basel, Switzerland)
Basel: MDPI., 12(2), 285.
https://doi.org/10.3390/antibiotics12020285

Barbakadze V, Merlani M, Gogilashvili L, Amiranashvili L, Petrou A, Geronikaki A, Ćirić A, Glamočlija J, Soković M. Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family. in Antibiotics (Basel, Switzerland). 2023;12(2):285.
doi:10.3390/antibiotics12020285 .

Barbakadze, Vakhtang, Merlani, Maia, Gogilashvili, Lali, Amiranashvili, Lela, Petrou, Anthi, Geronikaki, Athina, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family" in Antibiotics (Basel, Switzerland), 12, no. 2 (2023):285,
https://doi.org/10.3390/antibiotics12020285 . .

TY  - CONF
AU  - Carević, Tamara
AU  - Glamočlija, Jasmina
AU  - Nikolić, Biljana
AU  - Ivanov, Marija
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5332
AB  - The frequency of resistance to a large number of antimicrobial agents is increasing, so there
is a need to find new ones. A major problem in the treatment of infections caused by Candida
albicans is a highly resistant biofilm, which is a virulence factor of this species. The
mechanisms of action of selected flavonoids (hesperetin, sakuranetin, and taxifolin) on
species of the genus Candida were explored. The strains that were tested were C. albicans
10/15, C. albicans 13/15, C. albicans ATCC 10231, C. albicans 475/15, C. parapsilosis
ATCC 22019, C. tropicalis ATCC 750, C. krusei H1/16, and C. glabrata 4/6/15. The
antimicrobial potential of flavonoids was investigated by the microdilution method.
Determining the ability of these compounds to disrupt virulence factors, in the model of
Candida albicans, is based on monitoring the process of disrupting the formation and
destruction of previously formed biofilm. The cytotoxic effect on selected human cell lines
(lung fibroblasts) was investigated in order to determine whether flavonoids are selectively
toxic to species of the genus Candida, or cause non-selective toxicity in eukaryotic cells. The
laboratory methods used were microdilution method, antibiofilm method (crystal violet assay,
measures biofilm biomass), Congo red binding assay (measures the influence of compounds
on exopolysaccharide, EPS, production in biofilm), MTT cytotoxicity assay. The most
pronounced antifungal potential was observed for sakuranetin with a MIC of 0.041 mg/mL
for C. parapsilosis ATCC 22019 and a MIC of 0.082 mg/mL for the other strains tested. The
antimicrobial potential of hesperetin and taxifolin is the same for all tested strains; MIC value
is 0.165 mg/mL. All substances have been shown to be effective in inhibiting biofilm
formation, but also in destroying it. The antibiofilm effect of sakuranetin applied in
concentration equal to MIC against C. glabrata 4/6/15 and C. krusei H1/16 is over 85%.
These two strains are the most sensitive to the action of sakuranetin. The ability to destroy the
previously formed biofilm was pronounced towards C. albicans 475/15 (52.80%), and
moderate towards C. albicans ATCC 10231 (12.22%). The antibiofilm potential of hesperetin
is high for C. glabrata 4/6/15 (70.4%) and C. parapsilosis ATCC 22019 (62.6%), while for
other strains the percentage of inhibition is less than 50%, and for the formation of biofilm C.
krusei H1/16 hesperetin has no effect at all. The distinct effect of this flavonoid on the
destruction of previously formed biofilm is emphasized, with significant percentages of
destruction, while the percentages of EPS inhibition are low but significant (higher or closer
to the effect of ketoconazole). The most pronounced antibiofilm effect of taxifolin is
exhibited towards C. glabrata 4/6/15 (61.6%), while the percentage of inhibition is not higher
than 50% for other strains, which is generally lower effect than for other tested substances.
The cytotoxicity test indicates the following range of sensitivity of the examined cell line to
flavonoids: sakuranetin>hesperetin>taxifolin. The tested flavonoids have excellent potential
to be part of antifungal therapies, especially hesperetin, which has shown a strong antibiofilm
effect with pronounced selectivity towards pathogen cells.
PB  - Novi Sad: Matica srpska
C3  - Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia
T1  - Mechanisms of action of selected flavonoids towards species of the genus Candida
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5332
ER  - 

@conference{
author = "Carević, Tamara and Glamočlija, Jasmina and Nikolić, Biljana and Ivanov, Marija and Soković, Marina",
year = "2022",
abstract = "The frequency of resistance to a large number of antimicrobial agents is increasing, so there
is a need to find new ones. A major problem in the treatment of infections caused by Candida
albicans is a highly resistant biofilm, which is a virulence factor of this species. The
mechanisms of action of selected flavonoids (hesperetin, sakuranetin, and taxifolin) on
species of the genus Candida were explored. The strains that were tested were C. albicans
10/15, C. albicans 13/15, C. albicans ATCC 10231, C. albicans 475/15, C. parapsilosis
ATCC 22019, C. tropicalis ATCC 750, C. krusei H1/16, and C. glabrata 4/6/15. The
antimicrobial potential of flavonoids was investigated by the microdilution method.
Determining the ability of these compounds to disrupt virulence factors, in the model of
Candida albicans, is based on monitoring the process of disrupting the formation and
destruction of previously formed biofilm. The cytotoxic effect on selected human cell lines
(lung fibroblasts) was investigated in order to determine whether flavonoids are selectively
toxic to species of the genus Candida, or cause non-selective toxicity in eukaryotic cells. The
laboratory methods used were microdilution method, antibiofilm method (crystal violet assay,
measures biofilm biomass), Congo red binding assay (measures the influence of compounds
on exopolysaccharide, EPS, production in biofilm), MTT cytotoxicity assay. The most
pronounced antifungal potential was observed for sakuranetin with a MIC of 0.041 mg/mL
for C. parapsilosis ATCC 22019 and a MIC of 0.082 mg/mL for the other strains tested. The
antimicrobial potential of hesperetin and taxifolin is the same for all tested strains; MIC value
is 0.165 mg/mL. All substances have been shown to be effective in inhibiting biofilm
formation, but also in destroying it. The antibiofilm effect of sakuranetin applied in
concentration equal to MIC against C. glabrata 4/6/15 and C. krusei H1/16 is over 85%.
These two strains are the most sensitive to the action of sakuranetin. The ability to destroy the
previously formed biofilm was pronounced towards C. albicans 475/15 (52.80%), and
moderate towards C. albicans ATCC 10231 (12.22%). The antibiofilm potential of hesperetin
is high for C. glabrata 4/6/15 (70.4%) and C. parapsilosis ATCC 22019 (62.6%), while for
other strains the percentage of inhibition is less than 50%, and for the formation of biofilm C.
krusei H1/16 hesperetin has no effect at all. The distinct effect of this flavonoid on the
destruction of previously formed biofilm is emphasized, with significant percentages of
destruction, while the percentages of EPS inhibition are low but significant (higher or closer
to the effect of ketoconazole). The most pronounced antibiofilm effect of taxifolin is
exhibited towards C. glabrata 4/6/15 (61.6%), while the percentage of inhibition is not higher
than 50% for other strains, which is generally lower effect than for other tested substances.
The cytotoxicity test indicates the following range of sensitivity of the examined cell line to
flavonoids: sakuranetin>hesperetin>taxifolin. The tested flavonoids have excellent potential
to be part of antifungal therapies, especially hesperetin, which has shown a strong antibiofilm
effect with pronounced selectivity towards pathogen cells.",
publisher = "Novi Sad: Matica srpska",
journal = "Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia",
title = "Mechanisms of action of selected flavonoids towards species of the genus Candida",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5332"
}

Carević, T., Glamočlija, J., Nikolić, B., Ivanov, M.,& Soković, M.. (2022). Mechanisms of action of selected flavonoids towards species of the genus Candida. in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia
Novi Sad: Matica srpska., 46.
https://hdl.handle.net/21.15107/rcub_ibiss_5332

Carević T, Glamočlija J, Nikolić B, Ivanov M, Soković M. Mechanisms of action of selected flavonoids towards species of the genus Candida. in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia. 2022;:46.
https://hdl.handle.net/21.15107/rcub_ibiss_5332 .

Carević, Tamara, Glamočlija, Jasmina, Nikolić, Biljana, Ivanov, Marija, Soković, Marina, "Mechanisms of action of selected flavonoids towards species of the genus Candida" in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia (2022):46,
https://hdl.handle.net/21.15107/rcub_ibiss_5332 .

TY  - CONF
AU  - Stojković, Dejan
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Petrović, Jovana
AU  - Kostić, Marina
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5158
AB  - Chicory (Cichorium intybus L.) is an important industrial crop cultivated mainly to extract the
dietary fiber inulin. This study aimed to investigate antimicrobial, antibiofilm and wound healing
potentials of the extract obtained by supercritical fluid extraction (SFE). Antibacterial activity
of extract ranged from 0.25 to 4.00 mg/mL regarding minimal inhibitory concentra-tions (MICs),
while minimal bactericidal concentrations (MBCs) were noted within the range of 0.5 to 8.00 mg/
mL. The extract has shown promising antifungal potential with MICs rang-ing 0.125- 0.75 mg/
mL and MFCs 0.25-1.0 mg/mL against Candida species. Incubation with the extract has reduced
the ability of both C. albicans and P. aeruginosa to attach to the sur-face and begin the process
of biofilm formation. Concentration equal to previously deter-mined MIC has almost completely
(>90% inhibition) reduced the biofilm biomass formed by P. aeruginosa. Even twice as lower concentrations
(0.5 MIC) has limited the biofilm forming ability and induce more than 80% inhibition
in P. aeruginosa as bacterial biofilm model or-ganism. Impact on fungal biofilm was less profound
and MIC concentration was able to re-duce the biofilm formation for less than 60%. HaCaT (human
immortalized keratinocytes cell line) cells were treated with IC20 (24 h) concentration of chicory
SFE extract. It is estimated that SFE extract has slight effect on wound closure in keratinocyte
monolayer, when com-pared to untreated control. Since, Chicory SFE fraction possessed excellent
antibacterial po-tential against E. coli and K. pneumoniae and antifungal activity against Candida
species these results are considered very important in the future inventions
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5158
ER  - 

@conference{
author = "Stojković, Dejan and Ivanov, Marija and Ćirić, Ana and Petrović, Jovana and Kostić, Marina and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Chicory (Cichorium intybus L.) is an important industrial crop cultivated mainly to extract the
dietary fiber inulin. This study aimed to investigate antimicrobial, antibiofilm and wound healing
potentials of the extract obtained by supercritical fluid extraction (SFE). Antibacterial activity
of extract ranged from 0.25 to 4.00 mg/mL regarding minimal inhibitory concentra-tions (MICs),
while minimal bactericidal concentrations (MBCs) were noted within the range of 0.5 to 8.00 mg/
mL. The extract has shown promising antifungal potential with MICs rang-ing 0.125- 0.75 mg/
mL and MFCs 0.25-1.0 mg/mL against Candida species. Incubation with the extract has reduced
the ability of both C. albicans and P. aeruginosa to attach to the sur-face and begin the process
of biofilm formation. Concentration equal to previously deter-mined MIC has almost completely
(>90% inhibition) reduced the biofilm biomass formed by P. aeruginosa. Even twice as lower concentrations
(0.5 MIC) has limited the biofilm forming ability and induce more than 80% inhibition
in P. aeruginosa as bacterial biofilm model or-ganism. Impact on fungal biofilm was less profound
and MIC concentration was able to re-duce the biofilm formation for less than 60%. HaCaT (human
immortalized keratinocytes cell line) cells were treated with IC20 (24 h) concentration of chicory
SFE extract. It is estimated that SFE extract has slight effect on wound closure in keratinocyte
monolayer, when com-pared to untreated control. Since, Chicory SFE fraction possessed excellent
antibacterial po-tential against E. coli and K. pneumoniae and antifungal activity against Candida
species these results are considered very important in the future inventions",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5158"
}

Stojković, D., Ivanov, M., Ćirić, A., Petrović, J., Kostić, M., Glamočlija, J.,& Soković, M.. (2022). Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5158

Stojković D, Ivanov M, Ćirić A, Petrović J, Kostić M, Glamočlija J, Soković M. Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5158 .

Stojković, Dejan, Ivanov, Marija, Ćirić, Ana, Petrović, Jovana, Kostić, Marina, Glamočlija, Jasmina, Soković, Marina, "Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5158 .

TY  - CONF
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Kostić, Marina
AU  - Petrović, Jovana
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5581
AB  - Cichorium intybus L. (Asteraceae), is an important medicinal plant, rich in sesquiterpene
lactones (STLs), inulin, biologically relevant elements, and vitamins which exert potent
pro-health effects on the human organism. The aim of this study was to screen two
extracts of chicory containing STLs, the optimized extract (Ci_SFEopt) and the
supercritical fluid extract (Ci_SFEpur), for their antimicrobial activities against a range
of pathogenic microorganisms. Both extracts showed antibacterial activity, with
Ci_SFEpur being more potent than Ci_SFEopt. The most sensitive species was
Pseudomonas aeruginosa (minimal inhibitory concentration, MIC 0.06 mg/mL).
Ci_SFEpur and Ci_SFEopt extracts demonstrated good antifungal activity against
Candida species (MIC 0.03-1.00 mg/mL). Thin layer chromatography (TLC)
bioautography was used to screen the extracts and provide basic information for the
isolation of bioactive compounds in the future. Ci_SFEopt was active against all tested
microorganisms (Rf values were 0.18-0.88). The Ci_SFEpur extract efficiently inhibited
(Rf 0.76) Staphylococcus aureus, P. aeruginosa and Candida krusei. The identification
of powerful antimicrobial activity of Ci_SFEpur against P. aeruginosa and Candida
species is an important step towards the development of novel antimicrobial therapeutics
and efficient utilization of this widely distributed plant.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Evaluation of antimicrobial properties of chicory extracts
SP  - 257
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5581
ER  - 

@conference{
author = "Ćirić, Ana and Ivanov, Marija and Stojković, Dejan and Glamočlija, Jasmina and Kostić, Marina and Petrović, Jovana and Soković, Marina",
year = "2022",
abstract = "Cichorium intybus L. (Asteraceae), is an important medicinal plant, rich in sesquiterpene
lactones (STLs), inulin, biologically relevant elements, and vitamins which exert potent
pro-health effects on the human organism. The aim of this study was to screen two
extracts of chicory containing STLs, the optimized extract (Ci_SFEopt) and the
supercritical fluid extract (Ci_SFEpur), for their antimicrobial activities against a range
of pathogenic microorganisms. Both extracts showed antibacterial activity, with
Ci_SFEpur being more potent than Ci_SFEopt. The most sensitive species was
Pseudomonas aeruginosa (minimal inhibitory concentration, MIC 0.06 mg/mL).
Ci_SFEpur and Ci_SFEopt extracts demonstrated good antifungal activity against
Candida species (MIC 0.03-1.00 mg/mL). Thin layer chromatography (TLC)
bioautography was used to screen the extracts and provide basic information for the
isolation of bioactive compounds in the future. Ci_SFEopt was active against all tested
microorganisms (Rf values were 0.18-0.88). The Ci_SFEpur extract efficiently inhibited
(Rf 0.76) Staphylococcus aureus, P. aeruginosa and Candida krusei. The identification
of powerful antimicrobial activity of Ci_SFEpur against P. aeruginosa and Candida
species is an important step towards the development of novel antimicrobial therapeutics
and efficient utilization of this widely distributed plant.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Evaluation of antimicrobial properties of chicory extracts",
pages = "257",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5581"
}

Ćirić, A., Ivanov, M., Stojković, D., Glamočlija, J., Kostić, M., Petrović, J.,& Soković, M.. (2022). Evaluation of antimicrobial properties of chicory extracts. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 257.
https://hdl.handle.net/21.15107/rcub_ibiss_5581

Ćirić A, Ivanov M, Stojković D, Glamočlija J, Kostić M, Petrović J, Soković M. Evaluation of antimicrobial properties of chicory extracts. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:257.
https://hdl.handle.net/21.15107/rcub_ibiss_5581 .

Ćirić, Ana, Ivanov, Marija, Stojković, Dejan, Glamočlija, Jasmina, Kostić, Marina, Petrović, Jovana, Soković, Marina, "Evaluation of antimicrobial properties of chicory extracts" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):257,
https://hdl.handle.net/21.15107/rcub_ibiss_5581 .

TY  - CONF
AU  - Kostić, Marina
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5282
AB  - Veliki broj makrogljiva predstavlja nedovoljno iskorišćen izvor jedinjenja koja mogu imati potencijalnu primenu u medicini, farmaciji i različitim granama privrede. Pored toga što imaju povoljan odnos makronutrijenata, važan su izvor visoko cenjenih polisaharida, lektina, terpenoida, peptida, itd. Ova jedinjenja su odgovorna za mnogobrojne biološke aktivnosti uključujući antineurodegenerativnu, imunomodulatornu, antimikrobnu, antihiperglikemijsku aktivnost. Danas su u upotrebi različiti dijetetski proizvodi na bazi makrogljiva koji se koriste za prevenciju i ublažavanje simptoma različitih oboljenja. Iako ovi proizvodi ne mogu u potpunosti zameniti komercijalne lekove, u kombinaciji sa njihovom primenom mogu da dovedu do poboljšanja opšteg stanja. Zbog navedenog, autohtone samonikle, ali i komercijalno gajene vrste makrogljiva predstavljaju važan i još uvek nedovoljno istražen izvor bioloških aktivnih supstanci. Štaviše, one imaju ogroman potencijal kao profilaktički agensi kod bolesti čiji je osnovni uzrok povezan sa oksidativnim stresom, bakterijskim i gljivičnim infekcijama kao i pojavom/razvojem različitih tipova tumora. Imajući u vidu da su hranljiva i lekovita svojstva jestivih i lekovitih gljiva hvaljena kako u etnomedicini tako i u naučnim/kliničkim istraživanjima, ova svojstva će biti predstavljena kako bi se procenili novi trendovi i moguća primena jestivih i lekovitih gljiva sa teritorije Srbije u medicini, farmaceutskoj i prehrambenoj industriji.
AB  - Велики број макрогљива представља недовољно искоришћен извор једињења која могу имати потенцијалну примену у медицини, фармацији и различитим гранама привреде. Поред тога што имају повољан однос макронутријената, важан су извор високо цењених полисахарида, лектина, терпеноида, пептида, итд. Ова једињења су одговорна за многобројне биолошке активности укључујући антинеуродегенеративну, имуномодулаторну, антимикробну, антихипергликемијску активност. Данас су у употреби различити дијeтетски производи на бази макрогљива који се користе за превенцију и ублажавање симптома различитих обољења. Иако ови производи не могу у потпуности заменити комерцијалне лекове, у комбинацији са њиховом применом могу да доведу до побољшања општег стања. Због наведеног, аутохтоне самоникле, али и комерцијално гајене врсте макрогљива представљају важан и још увек недовољно истражен извор биолошких активних супстанци. Штавише, оне имају огроман потенцијал као профилактички агенси код болести чији је основни узрок повезан са оксидативним стресом, бактеријским и гљивичним инфекцијама као и појавом/развојем различитих типова тумора. Имајући у виду да су хранљива и лековита својства јестивих и лековитих гљива хваљена како у етномедицини тако и у научним/клиничким истраживањима, ова својства ће бити представљена како би се проценили нови трендови и могућа примена јестивих и лековитих гљива са територије Србије у медицини, фармацеутској и прехрамбеној индустрији.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Biološki potencijal gljiva sa teritorije Srbije
T1  - Биолошки потенцијал гљива са територије Србије
SP  - 227
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5282
ER  - 

@conference{
author = "Kostić, Marina and Petrović, Jovana and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Veliki broj makrogljiva predstavlja nedovoljno iskorišćen izvor jedinjenja koja mogu imati potencijalnu primenu u medicini, farmaciji i različitim granama privrede. Pored toga što imaju povoljan odnos makronutrijenata, važan su izvor visoko cenjenih polisaharida, lektina, terpenoida, peptida, itd. Ova jedinjenja su odgovorna za mnogobrojne biološke aktivnosti uključujući antineurodegenerativnu, imunomodulatornu, antimikrobnu, antihiperglikemijsku aktivnost. Danas su u upotrebi različiti dijetetski proizvodi na bazi makrogljiva koji se koriste za prevenciju i ublažavanje simptoma različitih oboljenja. Iako ovi proizvodi ne mogu u potpunosti zameniti komercijalne lekove, u kombinaciji sa njihovom primenom mogu da dovedu do poboljšanja opšteg stanja. Zbog navedenog, autohtone samonikle, ali i komercijalno gajene vrste makrogljiva predstavljaju važan i još uvek nedovoljno istražen izvor bioloških aktivnih supstanci. Štaviše, one imaju ogroman potencijal kao profilaktički agensi kod bolesti čiji je osnovni uzrok povezan sa oksidativnim stresom, bakterijskim i gljivičnim infekcijama kao i pojavom/razvojem različitih tipova tumora. Imajući u vidu da su hranljiva i lekovita svojstva jestivih i lekovitih gljiva hvaljena kako u etnomedicini tako i u naučnim/kliničkim istraživanjima, ova svojstva će biti predstavljena kako bi se procenili novi trendovi i moguća primena jestivih i lekovitih gljiva sa teritorije Srbije u medicini, farmaceutskoj i prehrambenoj industriji., Велики број макрогљива представља недовољно искоришћен извор једињења која могу имати потенцијалну примену у медицини, фармацији и различитим гранама привреде. Поред тога што имају повољан однос макронутријената, важан су извор високо цењених полисахарида, лектина, терпеноида, пептида, итд. Ова једињења су одговорна за многобројне биолошке активности укључујући антинеуродегенеративну, имуномодулаторну, антимикробну, антихипергликемијску активност. Данас су у употреби различити дијeтетски производи на бази макрогљива који се користе за превенцију и ублажавање симптома различитих обољења. Иако ови производи не могу у потпуности заменити комерцијалне лекове, у комбинацији са њиховом применом могу да доведу до побољшања општег стања. Због наведеног, аутохтоне самоникле, али и комерцијално гајене врсте макрогљива представљају важан и још увек недовољно истражен извор биолошких активних супстанци. Штавише, оне имају огроман потенцијал као профилактички агенси код болести чији је основни узрок повезан са оксидативним стресом, бактеријским и гљивичним инфекцијама као и појавом/развојем различитих типова тумора. Имајући у виду да су хранљива и лековита својства јестивих и лековитих гљива хваљена како у етномедицини тако и у научним/клиничким истраживањима, ова својства ће бити представљена како би се проценили нови трендови и могућа примена јестивих и лековитих гљива са територије Србије у медицини, фармацеутској и прехрамбеној индустрији.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Biološki potencijal gljiva sa teritorije Srbije, Биолошки потенцијал гљива са територије Србије",
pages = "227",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5282"
}

Kostić, M., Petrović, J., Glamočlija, J.,& Soković, M.. (2022). Biološki potencijal gljiva sa teritorije Srbije. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 227.
https://hdl.handle.net/21.15107/rcub_ibiss_5282

Kostić M, Petrović J, Glamočlija J, Soković M. Biološki potencijal gljiva sa teritorije Srbije. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:227.
https://hdl.handle.net/21.15107/rcub_ibiss_5282 .

Kostić, Marina, Petrović, Jovana, Glamočlija, Jasmina, Soković, Marina, "Biološki potencijal gljiva sa teritorije Srbije" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):227,
https://hdl.handle.net/21.15107/rcub_ibiss_5282 .

TY  - CONF
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Caleja, Cristina
AU  - Pereira, Eliana
AU  - Barros, Lillian
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5156
AB  - Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Chemical composition and biological activity of two Lactarius species from Serbia.
SP  - 102
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5156
ER  - 

@conference{
author = "Kostić, Marina and Ivanov, Marija and Ćirić, Ana and Caleja, Cristina and Pereira, Eliana and Barros, Lillian and Stojković, Dejan and Soković, Marina and Glamočlija, Jasmina",
year = "2022",
abstract = "Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Chemical composition and biological activity of two Lactarius species from Serbia.",
pages = "102-103",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5156"
}

Kostić, M., Ivanov, M., Ćirić, A., Caleja, C., Pereira, E., Barros, L., Stojković, D., Soković, M.,& Glamočlija, J.. (2022). Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156

Kostić M, Ivanov M, Ćirić A, Caleja C, Pereira E, Barros L, Stojković D, Soković M, Glamočlija J. Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

Kostić, Marina, Ivanov, Marija, Ćirić, Ana, Caleja, Cristina, Pereira, Eliana, Barros, Lillian, Stojković, Dejan, Soković, Marina, Glamočlija, Jasmina, "Chemical composition and biological activity of two Lactarius species from Serbia." in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):102-103,
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

TY  - JOUR
AU  - Pogrebnoi, Serghei
AU  - Radul, Oleg
AU  - Stingaci, Eugenia
AU  - Lupascu, Lucian
AU  - Valica, Vladimir
AU  - Uncu, Livia
AU  - Smetanscaia, Anastasia
AU  - Petrou, Anthi
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Geronikaki, Athina
AU  - Macaev, Fliur Z.
PY  - 2022
UR  - https://www.mdpi.com/2079-6382/11/5/588
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4982
AB  - The control of fungal pathogens is increasingly difficult due to the limited number of effective drugs available for antifungal therapy. In addition, both humans and fungi are eukaryotic organisms; antifungal drugs may have significant toxicity due to the inhibition of related human targets. Furthermore, another problem is increased incidents of fungal resistance to azoles, such as fluconazole, ketoconazole, voriconazole, etc. Thus, the interest in developing new azoles with an extended spectrum of activity still attracts the interest of the scientific community. Herein, we report the synthesis of a series of triazolium salts, an evaluation of their antifungal activity, and docking studies. Ketoconazole and bifonazole were used as reference drugs. All compounds showed good antifungal activity with MIC/MFC in the range of 0.0003 to 0.2/0.0006-0.4 mg/mL. Compound 19 exhibited the best activity among all tested with MIC/MFC in the range of 0.009 to 0.037 mg/mL and 0.0125-0.05 mg/mL, respectively. All compounds appeared to be more potent than both reference drugs. The docking studies are in accordance with experimental results.
PB  - Basel: MDPI
T2  - Antibiotics (Basel, Switzerland)
T1  - The Synthesis of Triazolium Salts as Antifungal Agents: A Biological and In Silico Evaluation.
IS  - 5
VL  - 11
DO  - 10.3390/antibiotics11050588
SP  - 588
ER  - 

@article{
author = "Pogrebnoi, Serghei and Radul, Oleg and Stingaci, Eugenia and Lupascu, Lucian and Valica, Vladimir and Uncu, Livia and Smetanscaia, Anastasia and Petrou, Anthi and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina and Geronikaki, Athina and Macaev, Fliur Z.",
year = "2022",
abstract = "The control of fungal pathogens is increasingly difficult due to the limited number of effective drugs available for antifungal therapy. In addition, both humans and fungi are eukaryotic organisms; antifungal drugs may have significant toxicity due to the inhibition of related human targets. Furthermore, another problem is increased incidents of fungal resistance to azoles, such as fluconazole, ketoconazole, voriconazole, etc. Thus, the interest in developing new azoles with an extended spectrum of activity still attracts the interest of the scientific community. Herein, we report the synthesis of a series of triazolium salts, an evaluation of their antifungal activity, and docking studies. Ketoconazole and bifonazole were used as reference drugs. All compounds showed good antifungal activity with MIC/MFC in the range of 0.0003 to 0.2/0.0006-0.4 mg/mL. Compound 19 exhibited the best activity among all tested with MIC/MFC in the range of 0.009 to 0.037 mg/mL and 0.0125-0.05 mg/mL, respectively. All compounds appeared to be more potent than both reference drugs. The docking studies are in accordance with experimental results.",
publisher = "Basel: MDPI",
journal = "Antibiotics (Basel, Switzerland)",
title = "The Synthesis of Triazolium Salts as Antifungal Agents: A Biological and In Silico Evaluation.",
number = "5",
volume = "11",
doi = "10.3390/antibiotics11050588",
pages = "588"
}

Pogrebnoi, S., Radul, O., Stingaci, E., Lupascu, L., Valica, V., Uncu, L., Smetanscaia, A., Petrou, A., Ćirić, A., Glamočlija, J., Soković, M., Geronikaki, A.,& Macaev, F. Z.. (2022). The Synthesis of Triazolium Salts as Antifungal Agents: A Biological and In Silico Evaluation.. in Antibiotics (Basel, Switzerland)
Basel: MDPI., 11(5), 588.
https://doi.org/10.3390/antibiotics11050588

Pogrebnoi S, Radul O, Stingaci E, Lupascu L, Valica V, Uncu L, Smetanscaia A, Petrou A, Ćirić A, Glamočlija J, Soković M, Geronikaki A, Macaev FZ. The Synthesis of Triazolium Salts as Antifungal Agents: A Biological and In Silico Evaluation.. in Antibiotics (Basel, Switzerland). 2022;11(5):588.
doi:10.3390/antibiotics11050588 .

Pogrebnoi, Serghei, Radul, Oleg, Stingaci, Eugenia, Lupascu, Lucian, Valica, Vladimir, Uncu, Livia, Smetanscaia, Anastasia, Petrou, Anthi, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, Geronikaki, Athina, Macaev, Fliur Z., "The Synthesis of Triazolium Salts as Antifungal Agents: A Biological and In Silico Evaluation." in Antibiotics (Basel, Switzerland), 11, no. 5 (2022):588,
https://doi.org/10.3390/antibiotics11050588 . .

TY  - JOUR
AU  - Kartsev, Victor
AU  - Geronikaki, Athina
AU  - Lichitsky, Boris
AU  - Komogortsev, Andrey
AU  - Petrou, Anthi
AU  - Ivanov, Marija
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/jhet.4491
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4962
AB  - Herein we report the design and synthesis of thiazolo[4,5-b]pyridin-5-ones and evaluation of their antimicrobial activity. The design was based on a molecular hybridization approach. Evaluation of their antibacterial activity revealed that these compounds generally showed moderate antibacterial activity. The best activity was achieved for compound 4p with MIC/MBC in the range of 0.12-0.47 and 0.23-0.94 mg mL(-1) respectively. Three compounds (4g, 4n, and 4p) were tested against three resistant strains, namely MRSA, p.aeruginosa, and E.coli, showing higher inhibition potential than the reference drug ampicillin. These three compounds also were tested for their ability to inhibit biofilm formation, with two of them showing better activity than streptomycin in a concentration of MIC (4p) and ampicillin in both concentrations (MIC and 0.5 MIC). As far as antifungal activity is concerned, the best activity was observed for compound 4i with MIC at 0.12-0.47 mg mL(-1) and MFC at 0.23-0.94 mg m(-1). According to docking studies, the predicted inhibition of E.coli MurB enzyme is probably a putative mechanism of the antibacterial activity of these compounds, while inhibition of 14a-lanosterol demethylase is probably the mechanism of their antifungal activity.
T2  - Journal of Heterocyclic Chemistry
T1  - Synthesis, biological evaluation and molecular docking studies of thiazolo[4,5‐ b ]pyridin‐5‐ones as antimicrobial agents
IS  - 9
VL  - 59
DO  - 10.1002/jhet.4491
SP  - 1573
EP  - 1590
ER  - 

@article{
author = "Kartsev, Victor and Geronikaki, Athina and Lichitsky, Boris and Komogortsev, Andrey and Petrou, Anthi and Ivanov, Marija and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Herein we report the design and synthesis of thiazolo[4,5-b]pyridin-5-ones and evaluation of their antimicrobial activity. The design was based on a molecular hybridization approach. Evaluation of their antibacterial activity revealed that these compounds generally showed moderate antibacterial activity. The best activity was achieved for compound 4p with MIC/MBC in the range of 0.12-0.47 and 0.23-0.94 mg mL(-1) respectively. Three compounds (4g, 4n, and 4p) were tested against three resistant strains, namely MRSA, p.aeruginosa, and E.coli, showing higher inhibition potential than the reference drug ampicillin. These three compounds also were tested for their ability to inhibit biofilm formation, with two of them showing better activity than streptomycin in a concentration of MIC (4p) and ampicillin in both concentrations (MIC and 0.5 MIC). As far as antifungal activity is concerned, the best activity was observed for compound 4i with MIC at 0.12-0.47 mg mL(-1) and MFC at 0.23-0.94 mg m(-1). According to docking studies, the predicted inhibition of E.coli MurB enzyme is probably a putative mechanism of the antibacterial activity of these compounds, while inhibition of 14a-lanosterol demethylase is probably the mechanism of their antifungal activity.",
journal = "Journal of Heterocyclic Chemistry",
title = "Synthesis, biological evaluation and molecular docking studies of thiazolo[4,5‐ b ]pyridin‐5‐ones as antimicrobial agents",
number = "9",
volume = "59",
doi = "10.1002/jhet.4491",
pages = "1573-1590"
}

Kartsev, V., Geronikaki, A., Lichitsky, B., Komogortsev, A., Petrou, A., Ivanov, M., Glamočlija, J.,& Soković, M.. (2022). Synthesis, biological evaluation and molecular docking studies of thiazolo[4,5‐ b ]pyridin‐5‐ones as antimicrobial agents. in Journal of Heterocyclic Chemistry, 59(9), 1573-1590.
https://doi.org/10.1002/jhet.4491

Kartsev V, Geronikaki A, Lichitsky B, Komogortsev A, Petrou A, Ivanov M, Glamočlija J, Soković M. Synthesis, biological evaluation and molecular docking studies of thiazolo[4,5‐ b ]pyridin‐5‐ones as antimicrobial agents. in Journal of Heterocyclic Chemistry. 2022;59(9):1573-1590.
doi:10.1002/jhet.4491 .

Kartsev, Victor, Geronikaki, Athina, Lichitsky, Boris, Komogortsev, Andrey, Petrou, Anthi, Ivanov, Marija, Glamočlija, Jasmina, Soković, Marina, "Synthesis, biological evaluation and molecular docking studies of thiazolo[4,5‐ b ]pyridin‐5‐ones as antimicrobial agents" in Journal of Heterocyclic Chemistry, 59, no. 9 (2022):1573-1590,
https://doi.org/10.1002/jhet.4491 . .

TY  - JOUR
AU  - Zubenko, Alexander
AU  - Kartsev, Victor
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Ivanov, Marija
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Divaeva, Lyudmila
AU  - Morkovnik, Anatolii
AU  - Klimenko, Alexander
PY  - 2022
UR  - https://www.mdpi.com/2079-6382/11/11/1654
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9686863
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5399
AB  - In this manuscript, we describe the design, preparation, and studies of antimicrobial activity of a series of novel heteroarylated benzothiazoles. A molecular hybridization approach was used for the designing compounds. The in vitro evaluation exposed that these compounds showed moderate antibacterial activity. Compound 2j was found to be the most potent (MIC/MBC at 0.23-0.94 mg/mL and 0.47-1.88 mg/mL) On the other hand, compounds showed good antifungal activity (MIC/MFC at 0.06-0.47 and 0.11-0.94 mg/mL respectively) with 2d being the most active one. The docking studies revealed that inhibition of E. coli MurB and 14-lanosterol demethylase probably represent the mechanism of antibacterial and antifungal activities.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Experimental and In Silico Evaluation of New Heteroaryl Benzothiazole Derivatives as Antimicrobial Agents
IS  - 11
VL  - 11
DO  - 10.3390/antibiotics11111654
SP  - 1654
ER  - 

@article{
author = "Zubenko, Alexander and Kartsev, Victor and Petrou, Anthi and Geronikaki, Athina and Ivanov, Marija and Glamočlija, Jasmina and Soković, Marina and Divaeva, Lyudmila and Morkovnik, Anatolii and Klimenko, Alexander",
year = "2022",
abstract = "In this manuscript, we describe the design, preparation, and studies of antimicrobial activity of a series of novel heteroarylated benzothiazoles. A molecular hybridization approach was used for the designing compounds. The in vitro evaluation exposed that these compounds showed moderate antibacterial activity. Compound 2j was found to be the most potent (MIC/MBC at 0.23-0.94 mg/mL and 0.47-1.88 mg/mL) On the other hand, compounds showed good antifungal activity (MIC/MFC at 0.06-0.47 and 0.11-0.94 mg/mL respectively) with 2d being the most active one. The docking studies revealed that inhibition of E. coli MurB and 14-lanosterol demethylase probably represent the mechanism of antibacterial and antifungal activities.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Experimental and In Silico Evaluation of New Heteroaryl Benzothiazole Derivatives as Antimicrobial Agents",
number = "11",
volume = "11",
doi = "10.3390/antibiotics11111654",
pages = "1654"
}

Zubenko, A., Kartsev, V., Petrou, A., Geronikaki, A., Ivanov, M., Glamočlija, J., Soković, M., Divaeva, L., Morkovnik, A.,& Klimenko, A.. (2022). Experimental and In Silico Evaluation of New Heteroaryl Benzothiazole Derivatives as Antimicrobial Agents. in Antibiotics
Basel: MDPI., 11(11), 1654.
https://doi.org/10.3390/antibiotics11111654

Zubenko A, Kartsev V, Petrou A, Geronikaki A, Ivanov M, Glamočlija J, Soković M, Divaeva L, Morkovnik A, Klimenko A. Experimental and In Silico Evaluation of New Heteroaryl Benzothiazole Derivatives as Antimicrobial Agents. in Antibiotics. 2022;11(11):1654.
doi:10.3390/antibiotics11111654 .

Zubenko, Alexander, Kartsev, Victor, Petrou, Anthi, Geronikaki, Athina, Ivanov, Marija, Glamočlija, Jasmina, Soković, Marina, Divaeva, Lyudmila, Morkovnik, Anatolii, Klimenko, Alexander, "Experimental and In Silico Evaluation of New Heteroaryl Benzothiazole Derivatives as Antimicrobial Agents" in Antibiotics, 11, no. 11 (2022):1654,
https://doi.org/10.3390/antibiotics11111654 . .

TY  - JOUR
AU  - Kartsev, Victor
AU  - Geronikaki, Athina
AU  - Zubenko, Alexander
AU  - Petrou, Anthi
AU  - Ivanov, Marija
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Divaeva, Lyudmila
AU  - Morkovnik, Anatolii
AU  - Klimenko, Alexander
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9658463
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5173
AB  - Herein, we report the design, synthesis, and evaluation of the antimicrobial activity of new heteroaryl (aryl) thiazole derivatives. The design was based on a molecular hybridization approach. The in vitro evaluation revealed that these compounds demonstrated moderate antibacterial activity. The best activity was achieved for compound 3, with MIC and MBC in the range of 0.23-0.7 and 0.47-0.94 mg/mL, respectively. Three compounds (2, 3, and 4) were tested against three resistant strains, namely methicillin resistant Staphylococcus aureus, P. aeruginosa, and E. coli, which showed higher potential than the reference drug ampicillin. Antifungal activity of the compounds was better with MIC and MFC in the range of 0.06-0.47 and 0.11-0.94 mg/mL, respectively. The best activity was observed for compound 9, with MIC at 0.06-0.23 mg/mL and MFC at 0.11-0.47 mg/mL. According to docking studies, the predicted inhibition of the E. coli MurB enzyme is a putative mechanism of the antibacterial activity of the compounds, while inhibition of 14a-lanosterol demethylase is probably the mechanism of their antifungal activity.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Synthesis and Antimicrobial Activity of New Heteroaryl(aryl) Thiazole Derivatives Molecular Docking Studies
IS  - 10
VL  - 11
DO  - 10.3390/antibiotics11101337
SP  - 1337
ER  - 

@article{
author = "Kartsev, Victor and Geronikaki, Athina and Zubenko, Alexander and Petrou, Anthi and Ivanov, Marija and Glamočlija, Jasmina and Soković, Marina and Divaeva, Lyudmila and Morkovnik, Anatolii and Klimenko, Alexander",
year = "2022",
abstract = "Herein, we report the design, synthesis, and evaluation of the antimicrobial activity of new heteroaryl (aryl) thiazole derivatives. The design was based on a molecular hybridization approach. The in vitro evaluation revealed that these compounds demonstrated moderate antibacterial activity. The best activity was achieved for compound 3, with MIC and MBC in the range of 0.23-0.7 and 0.47-0.94 mg/mL, respectively. Three compounds (2, 3, and 4) were tested against three resistant strains, namely methicillin resistant Staphylococcus aureus, P. aeruginosa, and E. coli, which showed higher potential than the reference drug ampicillin. Antifungal activity of the compounds was better with MIC and MFC in the range of 0.06-0.47 and 0.11-0.94 mg/mL, respectively. The best activity was observed for compound 9, with MIC at 0.06-0.23 mg/mL and MFC at 0.11-0.47 mg/mL. According to docking studies, the predicted inhibition of the E. coli MurB enzyme is a putative mechanism of the antibacterial activity of the compounds, while inhibition of 14a-lanosterol demethylase is probably the mechanism of their antifungal activity.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Synthesis and Antimicrobial Activity of New Heteroaryl(aryl) Thiazole Derivatives Molecular Docking Studies",
number = "10",
volume = "11",
doi = "10.3390/antibiotics11101337",
pages = "1337"
}

Kartsev, V., Geronikaki, A., Zubenko, A., Petrou, A., Ivanov, M., Glamočlija, J., Soković, M., Divaeva, L., Morkovnik, A.,& Klimenko, A.. (2022). Synthesis and Antimicrobial Activity of New Heteroaryl(aryl) Thiazole Derivatives Molecular Docking Studies. in Antibiotics
Basel: MDPI., 11(10), 1337.
https://doi.org/10.3390/antibiotics11101337

Kartsev V, Geronikaki A, Zubenko A, Petrou A, Ivanov M, Glamočlija J, Soković M, Divaeva L, Morkovnik A, Klimenko A. Synthesis and Antimicrobial Activity of New Heteroaryl(aryl) Thiazole Derivatives Molecular Docking Studies. in Antibiotics. 2022;11(10):1337.
doi:10.3390/antibiotics11101337 .

Kartsev, Victor, Geronikaki, Athina, Zubenko, Alexander, Petrou, Anthi, Ivanov, Marija, Glamočlija, Jasmina, Soković, Marina, Divaeva, Lyudmila, Morkovnik, Anatolii, Klimenko, Alexander, "Synthesis and Antimicrobial Activity of New Heteroaryl(aryl) Thiazole Derivatives Molecular Docking Studies" in Antibiotics, 11, no. 10 (2022):1337,
https://doi.org/10.3390/antibiotics11101337 . .