TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Fernandes, Ângela
AU  - Calhelha, Ricardo C.
AU  - Glamočlija, Jasmina
AU  - Barros, Lillian
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2023
UR  - https://www.mdpi.com/2309-608X/9/1/70
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9864295
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5461
AB  - Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).
PB  - Basel: MDPI
T2  - Journal of Fungi
T1  - A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity
IS  - 1
VL  - 9
DO  - 10.3390/jof9010070
SP  - 70
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Fernandes, Ângela and Calhelha, Ricardo C. and Glamočlija, Jasmina and Barros, Lillian and Soković, Marina and Ćirić, Ana",
year = "2023",
abstract = "Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).",
publisher = "Basel: MDPI",
journal = "Journal of Fungi",
title = "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity",
number = "1",
volume = "9",
doi = "10.3390/jof9010070",
pages = "70"
}

Kostić, M., Ivanov, M., Fernandes, Â., Calhelha, R. C., Glamočlija, J., Barros, L., Soković, M.,& Ćirić, A.. (2023). A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi
Basel: MDPI., 9(1), 70.
https://doi.org/10.3390/jof9010070

Kostić M, Ivanov M, Fernandes Â, Calhelha RC, Glamočlija J, Barros L, Soković M, Ćirić A. A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi. 2023;9(1):70.
doi:10.3390/jof9010070 .

Kostić, Marina, Ivanov, Marija, Fernandes, Ângela, Calhelha, Ricardo C., Glamočlija, Jasmina, Barros, Lillian, Soković, Marina, Ćirić, Ana, "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity" in Journal of Fungi, 9, no. 1 (2023):70,
https://doi.org/10.3390/jof9010070 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5818
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
DO  - 10.3389/fevo.2023.1148309
SP  - 1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
doi = "10.3389/fevo.2023.1148309",
pages = "1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Merlani, Maia
AU  - Nadaraia, Nanuli
AU  - Amiranashvili, Lela
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Carević, Tamara
AU  - Soković, Marina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5659
AB  - Twelve steroid based hydrazones were in silico evaluated using computer program PASS as antimicrobial agents. The experimental evaluation revealed that all compounds have low to moderate antibacterial activity against all bacteria tested, except for B.cereus with MIC at a range of 0.37–3.00 mg/mL and MBC at 0.75–6.00 mg/mL. The most potent appeared to be compound 11 with MIC/MBC of0.75/1.5 mg/mL, respectively. The evaluation of antibacterial activity against three resistant strains MRSA, E.coli and P.aeruginosa demonstrated superior activity of compounds against MRSA compared with ampicillin, which did not show bacteriostatic or bactericidal activities. All compounds exhibited good antifungal activity with MIC of 0.37–1.50 mg/mL and MFC of 1.50–3.00 mg/mL, but with different sensitivity against fungi tested. According to docking studies, 14-alpha demethylase inhibition may be responsible for antifungal activity. Two compounds were evaluated for their antibiofilm activity. Finally, drug-likeness and docking prediction were performed.
PB  - Basel: MDPI
T2  - Molecules
T1  - Antimicrobial Activity of Some Steroidal Hydrazones
IS  - 3
VL  - 28
DO  - 10.3390/molecules28031167
SP  - 1167
ER  - 

@article{
author = "Merlani, Maia and Nadaraia, Nanuli and Amiranashvili, Lela and Petrou, Anthi and Geronikaki, Athina and Ćirić, Ana and Glamočlija, Jasmina and Carević, Tamara and Soković, Marina",
year = "2023",
abstract = "Twelve steroid based hydrazones were in silico evaluated using computer program PASS as antimicrobial agents. The experimental evaluation revealed that all compounds have low to moderate antibacterial activity against all bacteria tested, except for B.cereus with MIC at a range of 0.37–3.00 mg/mL and MBC at 0.75–6.00 mg/mL. The most potent appeared to be compound 11 with MIC/MBC of0.75/1.5 mg/mL, respectively. The evaluation of antibacterial activity against three resistant strains MRSA, E.coli and P.aeruginosa demonstrated superior activity of compounds against MRSA compared with ampicillin, which did not show bacteriostatic or bactericidal activities. All compounds exhibited good antifungal activity with MIC of 0.37–1.50 mg/mL and MFC of 1.50–3.00 mg/mL, but with different sensitivity against fungi tested. According to docking studies, 14-alpha demethylase inhibition may be responsible for antifungal activity. Two compounds were evaluated for their antibiofilm activity. Finally, drug-likeness and docking prediction were performed.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Antimicrobial Activity of Some Steroidal Hydrazones",
number = "3",
volume = "28",
doi = "10.3390/molecules28031167",
pages = "1167"
}

Merlani, M., Nadaraia, N., Amiranashvili, L., Petrou, A., Geronikaki, A., Ćirić, A., Glamočlija, J., Carević, T.,& Soković, M.. (2023). Antimicrobial Activity of Some Steroidal Hydrazones. in Molecules
Basel: MDPI., 28(3), 1167.
https://doi.org/10.3390/molecules28031167

Merlani M, Nadaraia N, Amiranashvili L, Petrou A, Geronikaki A, Ćirić A, Glamočlija J, Carević T, Soković M. Antimicrobial Activity of Some Steroidal Hydrazones. in Molecules. 2023;28(3):1167.
doi:10.3390/molecules28031167 .

Merlani, Maia, Nadaraia, Nanuli, Amiranashvili, Lela, Petrou, Anthi, Geronikaki, Athina, Ćirić, Ana, Glamočlija, Jasmina, Carević, Tamara, Soković, Marina, "Antimicrobial Activity of Some Steroidal Hydrazones" in Molecules, 28, no. 3 (2023):1167,
https://doi.org/10.3390/molecules28031167 . .

TY  - JOUR
AU  - Petrou, Anthi
AU  - Kartsev, Victor
AU  - Geronikaki, Athina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Soković, Marina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5796
AB  - Nine new functionally substituted derivatives of 2-aminothiazole 
were evaluated for antimicrobial activity using microdilution 
method against the panel of eight bacterial and eight fungal strains. 
Evaluation of antibacterial activity revealed that compounds are 
potent antibacterial agents, more active than ampicillin and strep tomycin except of some compounds against B. cereus and En. 
cloacae. The best compound appeared to be compound 8. The 
most sensitive bacteria appeared to be En. cloacae, while L. mono cytogenes was the most resistant. Compounds also exhibited good 
antifungal activity much better than two reference drugs, ketoco nazole and bifonazole. Compound 1 exhibited the best antifungal 
activity. The most sensitive fungus was T. viride, while A. fumigatus 
was the most resistant. Bacteria as well as fungi in general showed 
different sensitivity towards compounds tested. Molecular docking 
studies revealed that MurB inhibition is probably involved in the 
mechanism of antibacterial activity, while CYP51 of C. albicans is 
responsible for the mechanism of antifungal activity. Finally, it 
should be mentioned that all compounds displayed very good 
druglikeness scores
PB  - Taylor & Francis Group
T2  - SAR and QSAR in Environmental Research
T1  - Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation
IS  - 5
VL  - 34
DO  - 10.1080/1062936X.2023.2214869
SP  - 359
EP  - 414
ER  - 

@article{
author = "Petrou, Anthi and Kartsev, Victor and Geronikaki, Athina and Glamočlija, Jasmina and Ćirić, Ana and Soković, Marina",
year = "2023",
abstract = "Nine new functionally substituted derivatives of 2-aminothiazole 
were evaluated for antimicrobial activity using microdilution 
method against the panel of eight bacterial and eight fungal strains. 
Evaluation of antibacterial activity revealed that compounds are 
potent antibacterial agents, more active than ampicillin and strep tomycin except of some compounds against B. cereus and En. 
cloacae. The best compound appeared to be compound 8. The 
most sensitive bacteria appeared to be En. cloacae, while L. mono cytogenes was the most resistant. Compounds also exhibited good 
antifungal activity much better than two reference drugs, ketoco nazole and bifonazole. Compound 1 exhibited the best antifungal 
activity. The most sensitive fungus was T. viride, while A. fumigatus 
was the most resistant. Bacteria as well as fungi in general showed 
different sensitivity towards compounds tested. Molecular docking 
studies revealed that MurB inhibition is probably involved in the 
mechanism of antibacterial activity, while CYP51 of C. albicans is 
responsible for the mechanism of antifungal activity. Finally, it 
should be mentioned that all compounds displayed very good 
druglikeness scores",
publisher = "Taylor & Francis Group",
journal = "SAR and QSAR in Environmental Research",
title = "Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation",
number = "5",
volume = "34",
doi = "10.1080/1062936X.2023.2214869",
pages = "359-414"
}

Petrou, A., Kartsev, V., Geronikaki, A., Glamočlija, J., Ćirić, A.,& Soković, M.. (2023). Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation. in SAR and QSAR in Environmental Research
Taylor & Francis Group., 34(5), 359-414.
https://doi.org/10.1080/1062936X.2023.2214869

Petrou A, Kartsev V, Geronikaki A, Glamočlija J, Ćirić A, Soković M. Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation. in SAR and QSAR in Environmental Research. 2023;34(5):359-414.
doi:10.1080/1062936X.2023.2214869 .

Petrou, Anthi, Kartsev, Victor, Geronikaki, Athina, Glamočlija, Jasmina, Ćirić, Ana, Soković, Marina, "Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation" in SAR and QSAR in Environmental Research, 34, no. 5 (2023):359-414,
https://doi.org/10.1080/1062936X.2023.2214869 . .

TY  - CONF
AU  - Rajaković, Mladen
AU  - Gašić, Uroš
AU  - Bukvički, Danka
AU  - Pešić, Mirjana
AU  - Ćirić, Ana
AU  - Milinčić, Danijel
AU  - Stojković, Dejan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6437
AB  - According to recent research, a variety of inedible portions of fruits and vegetables, as well as food waste, are excellent sources of phytochemicals that may be isolated and reintroduced as natural food additives into the food chain [1]. Besides the food industry, natural product from non-edible parts of the fruits of Cucurbitaceae family can be used in other industries such as cosmetic and pharmaceutical and the fact that the plants in this family are also used medicinally makes them excellent research subjects for the study of plants with high therapeutic potential [2]. The focus of our study was the solid/liquid extraction of lyophilized fruit peels of Cucumis metuliferus E. Mey. (Cucurbitaceae) under different extraction conditions such as extraction time (min), ethanol/water ratio (%) and power of ultrasonic bath (%). It was aimed as well to determine the phytochemical composition of 25 different ethanolic and hydro-ethanolic extracts by performing UHPLC-QToF-MS analysis. The Figure 1 shows the fruit of Horned Melon (C. metuliferus) and the exocarp as part of fruit used for extraction. Under different extraction conditions percentage yields of the dry extracts varied between 26.37% - 43.98%, obtaining the optimal extraction conditions as follows: EtOH (%): 50; Amplitude (%): 40; Time (min): 30. Furthermore, chemical profiling of the extracts revealed the presence of the following compounds: p-hydroxybenzoic acid, dihydroxybenzoic acid, galloyl pentoside, dihydroxybenzoyl pentoside, hydroxybenzoyl hexoside, vanilloyl hexoside and hydroxybenzoyl rhamnosyl hexoside. This is the first study that highlights the optimization of extraction conditions of the C. metuliferus peels, as well as chemical identification of bioactive compounds present in the extracts. Our future observations will be oriented towards exploring bioactive properties of the obtained extracts and further incorporations in food matrices.
PB  - Belgrade: Serbian Chemical Society
C3  - Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia
T1  - Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels
SP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6437
ER  - 

@conference{
author = "Rajaković, Mladen and Gašić, Uroš and Bukvički, Danka and Pešić, Mirjana and Ćirić, Ana and Milinčić, Danijel and Stojković, Dejan",
year = "2023",
abstract = "According to recent research, a variety of inedible portions of fruits and vegetables, as well as food waste, are excellent sources of phytochemicals that may be isolated and reintroduced as natural food additives into the food chain [1]. Besides the food industry, natural product from non-edible parts of the fruits of Cucurbitaceae family can be used in other industries such as cosmetic and pharmaceutical and the fact that the plants in this family are also used medicinally makes them excellent research subjects for the study of plants with high therapeutic potential [2]. The focus of our study was the solid/liquid extraction of lyophilized fruit peels of Cucumis metuliferus E. Mey. (Cucurbitaceae) under different extraction conditions such as extraction time (min), ethanol/water ratio (%) and power of ultrasonic bath (%). It was aimed as well to determine the phytochemical composition of 25 different ethanolic and hydro-ethanolic extracts by performing UHPLC-QToF-MS analysis. The Figure 1 shows the fruit of Horned Melon (C. metuliferus) and the exocarp as part of fruit used for extraction. Under different extraction conditions percentage yields of the dry extracts varied between 26.37% - 43.98%, obtaining the optimal extraction conditions as follows: EtOH (%): 50; Amplitude (%): 40; Time (min): 30. Furthermore, chemical profiling of the extracts revealed the presence of the following compounds: p-hydroxybenzoic acid, dihydroxybenzoic acid, galloyl pentoside, dihydroxybenzoyl pentoside, hydroxybenzoyl hexoside, vanilloyl hexoside and hydroxybenzoyl rhamnosyl hexoside. This is the first study that highlights the optimization of extraction conditions of the C. metuliferus peels, as well as chemical identification of bioactive compounds present in the extracts. Our future observations will be oriented towards exploring bioactive properties of the obtained extracts and further incorporations in food matrices.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia",
title = "Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels",
pages = "171",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6437"
}

Rajaković, M., Gašić, U., Bukvički, D., Pešić, M., Ćirić, A., Milinčić, D.,& Stojković, D.. (2023). Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels. in Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia
Belgrade: Serbian Chemical Society., 171.
https://hdl.handle.net/21.15107/rcub_ibiss_6437

Rajaković M, Gašić U, Bukvički D, Pešić M, Ćirić A, Milinčić D, Stojković D. Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels. in Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia. 2023;:171.
https://hdl.handle.net/21.15107/rcub_ibiss_6437 .

Rajaković, Mladen, Gašić, Uroš, Bukvički, Danka, Pešić, Mirjana, Ćirić, Ana, Milinčić, Danijel, Stojković, Dejan, "Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels" in Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia (2023):171,
https://hdl.handle.net/21.15107/rcub_ibiss_6437 .

TY  - CONF
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Petrović, Jovana
AU  - Stojković, Dejan
AU  - Gašić, Uroš
AU  - Milinčić, Danijel
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6463
AB  - Introduction: The food industry increasingly utilizes plant extracts over synthetic antioxidants for preventing lipid oxidation, fighting foodborne pathogens, and prolonging shelf life of various products. The bioactive potential varies among plant species. Traditionally, extracts from rosehip fruits (Rosa canina L.) have preventive and curative effects on diabetes, memory disorders and cancer. In addition, these extracts are often used as antimicrobial, antinociceptive and anti-inflammatory agents.
Objective: The aim of this work was to investigate phytochemical composition and biological potential of aqueous extracts from the fruits of R. canina. 
Methodology: The lyophilized aqueous extract was analyzed using Agilent 1290 Infinity ultra-high performance liquid chromatography (UHPLC) system in conjunction with quadrupole time-of-flight mass spectrometry. The extracts were analyzed for total polyphenol and flavonoid content (TPC and TFC, respectively) using spectrophotometric methods. The biological activities of aqueous extracts were evaluated under in vitro conditions. The Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assay were used to investigate antioxidant potential. Antimicrobial activity was determined using microdilution assay and crystal violet and MTT tests were used for evaluation of antibiofilm activity.
Results and conclusions: R. canina fruit's aqueous extract had 32 phenolic compounds, which were classified into eight classes. Qualitative phytochemical analyses showed that this extract contained flavan-3-ols and procyanidins, dominated by epicatechin (624.354 mg/100g) and a proanthocyanidin dimer hexoside type B (457.016 mg/100g). Furthermore, extract was high in total phenolic content (412.25 TE mg/g) but low in total flavonoid compounds (0.73 QE mg/g). The highest antioxidant capacity was measured by FRAP assay (103.29 AAE mg/g), whereas notable antimicrobial properties were determined as well, with a standout 67.68% antibiofilm activity against Candida spp. at 0.5 MIC. These findings highlight potential of R. canina as a dietary source of antioxidants and antimicrobial agents.
PB  - Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia
T1  - Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6463
ER  - 

@conference{
author = "Ćirić, Ana and Ivanov, Marija and Petrović, Jovana and Stojković, Dejan and Gašić, Uroš and Milinčić, Danijel and Pešić, Mirjana",
year = "2023",
abstract = "Introduction: The food industry increasingly utilizes plant extracts over synthetic antioxidants for preventing lipid oxidation, fighting foodborne pathogens, and prolonging shelf life of various products. The bioactive potential varies among plant species. Traditionally, extracts from rosehip fruits (Rosa canina L.) have preventive and curative effects on diabetes, memory disorders and cancer. In addition, these extracts are often used as antimicrobial, antinociceptive and anti-inflammatory agents.
Objective: The aim of this work was to investigate phytochemical composition and biological potential of aqueous extracts from the fruits of R. canina. 
Methodology: The lyophilized aqueous extract was analyzed using Agilent 1290 Infinity ultra-high performance liquid chromatography (UHPLC) system in conjunction with quadrupole time-of-flight mass spectrometry. The extracts were analyzed for total polyphenol and flavonoid content (TPC and TFC, respectively) using spectrophotometric methods. The biological activities of aqueous extracts were evaluated under in vitro conditions. The Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assay were used to investigate antioxidant potential. Antimicrobial activity was determined using microdilution assay and crystal violet and MTT tests were used for evaluation of antibiofilm activity.
Results and conclusions: R. canina fruit's aqueous extract had 32 phenolic compounds, which were classified into eight classes. Qualitative phytochemical analyses showed that this extract contained flavan-3-ols and procyanidins, dominated by epicatechin (624.354 mg/100g) and a proanthocyanidin dimer hexoside type B (457.016 mg/100g). Furthermore, extract was high in total phenolic content (412.25 TE mg/g) but low in total flavonoid compounds (0.73 QE mg/g). The highest antioxidant capacity was measured by FRAP assay (103.29 AAE mg/g), whereas notable antimicrobial properties were determined as well, with a standout 67.68% antibiofilm activity against Candida spp. at 0.5 MIC. These findings highlight potential of R. canina as a dietary source of antioxidants and antimicrobial agents.",
publisher = "Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia",
title = "Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6463"
}

Ćirić, A., Ivanov, M., Petrović, J., Stojković, D., Gašić, U., Milinčić, D.,& Pešić, M.. (2023). Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications. in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia
Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_6463

Ćirić A, Ivanov M, Petrović J, Stojković D, Gašić U, Milinčić D, Pešić M. Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications. in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia. 2023;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_6463 .

Ćirić, Ana, Ivanov, Marija, Petrović, Jovana, Stojković, Dejan, Gašić, Uroš, Milinčić, Danijel, Pešić, Mirjana, "Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications" in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia (2023):41,
https://hdl.handle.net/21.15107/rcub_ibiss_6463 .

TY  - JOUR
AU  - Mileski, Ksenija S.
AU  - Ćirić, Ana
AU  - Gašić, Uroš
AU  - Žarković, Lazar D.
AU  - Krivošej, Zoran Đ.
AU  - Džamić, Ana M.
PY  - 2023
UR  - https://www.acgpubs.org/doc/20221125121922364-RNP-2207-2528.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5345
AB  - The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.
T2  - Records of Natural Products
T1  - Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia
IS  - 3
VL  - 17
DO  - 10.25135/rnp.364.2207.2528
SP  - 453
EP  - 475
ER  - 

@article{
author = "Mileski, Ksenija S. and Ćirić, Ana and Gašić, Uroš and Žarković, Lazar D. and Krivošej, Zoran Đ. and Džamić, Ana M.",
year = "2023",
abstract = "The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.",
journal = "Records of Natural Products",
title = "Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia",
number = "3",
volume = "17",
doi = "10.25135/rnp.364.2207.2528",
pages = "453-475"
}

Mileski, K. S., Ćirić, A., Gašić, U., Žarković, L. D., Krivošej, Z. Đ.,& Džamić, A. M.. (2023). Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia. in Records of Natural Products, 17(3), 453-475.
https://doi.org/10.25135/rnp.364.2207.2528

Mileski KS, Ćirić A, Gašić U, Žarković LD, Krivošej ZĐ, Džamić AM. Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia. in Records of Natural Products. 2023;17(3):453-475.
doi:10.25135/rnp.364.2207.2528 .

Mileski, Ksenija S., Ćirić, Ana, Gašić, Uroš, Žarković, Lazar D., Krivošej, Zoran Đ., Džamić, Ana M., "Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia" in Records of Natural Products, 17, no. 3 (2023):453-475,
https://doi.org/10.25135/rnp.364.2207.2528 . .

TY  - CONF
AU  - Carević, Tamara
AU  - Petrović, Jovana
AU  - Ivanov, Marija
AU  - Milinčić, Danijel
AU  - Pešić, Mirjana
AU  - Ćirić, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6391
AB  - Kontinuirani rast tržišta piva i alkoholnih pića otvorio je nove mogućnosti za poboljšanje ukusa, arome i korisnosti ovih napitaka. Već hiljadu godina unazad je poznato da dodavanje različitih biljnih sastojaka u piće menja njihov ukus i svojstva. Međutim, moderna tehnologija olakšava postizanje željenih karakteristika i nudi nove mogućnosti za terapeutske efekte i senzorna poboljšanja ovog tradicionalnog pića.
U ovom radu su predstavljene hemijska analiza i bioaktivna svojstva etanolnog i vodenog ekstrakta Prunus spinosa L. (trnjine) kao potencijalnog dodatka pivu, sa ciljem povećanja funkcionalnih karakteristika i senzornih osobina ovog napitka.
Hemijske analize korišćenjem UHPLC-QToF-MS pokazale su prisustvo: derivata hidroksibenzoeve kiseline (heksozid vanilinske kiseline do 141.476 mg/100 g ekstrakta), derivata hidroksicimetne kiseline (izomer kafeoilkvinske kiseline do 306.608 mg/100 g ekstrakta, feruloilkvinske kiseline do 225.884 mg/100 g ekstrakta) i derivata flavona (apigenin 180.094 mg/100g ekstrakta).
Procena bioaktivnih svojstava nesumnjivo je pokazala da oba ekstrakta trnjine imaju umeren do dobar antimikrobni potencijal, kao i sposobnost da inhibiraju formiranje biofilma, pri čemu je etanolni ekstrakt pokazao sveukupni bolji potencijal u inhibiciji rasta mikroorganizama. Najbolji antimikrobni potencijal je pokazan kod vodenog ekstrakta prema Bacillus cereus koja je poznati patogen identifikovan u namirnicama (MIC 0.5 mg/mL, MBC 1.00 mg/mL), dok su druge testirane bakterije bile otpornije na aktivnost testiranih ekstrakata (MIC u opsegu od 1.0- 4.0 mg/mL i MBC u opsegu od 2.00-8.00 mg/mL). Rezultati antifungalne aktivnosti su pokazali da ekstrakti prilično ujednačeno deluju inhibitorno na rast testiranih mikrogljiva, uz MIK vrednosti 1.00 mg/mL i MFK 2.00 mg/mL. Detaljnija analiza antimikrobne aktivnosti pokazala je da testirani ekstrakti trnjine značajno inhibiraju formiranje biofilma Candida albicans ATCC 10231; oba testirana uzorka su inhibirala formiranje biofilma na subinhibitornim koncentracijama približno 67.00 %. Uočena aktivnost se može pripisati prisustvu različitih fenolnih jedinjenja.
Imajući u vidu poznat gorak ukus P. spinosa kao i pokazana bioaktivna svojstva njegovih ekstrakata, verujemo da bi ova biljka bila odličan funkcionalni sastojak koji će se dobro uklopiti u jedinstvena senzorna svojstva piva i sličnih napitaka. Predloženo pivo bi čak moglo da pruži dodatne zdravstvene efekte potrošačima, balansirajući između zadovoljstva i njihove želje za zdravim sastojcima.
AB  - The continuous growth of beer and beverage market has opened new opportunities to improve their flavor, aroma, and benefits. It has been known for thousands of years that adding various herbs to beer changes the taste and properties of this beverage. However, modern technology makes it easier to achieve the desired properties and provides useful qualities for therapeutic effects and sensory improvement of traditional beer. This paper presents chemical analysis and bioactive properties of ethanolic and aqueous extract of Prunus spinosa L. (blackthorn) as a potential ingredient for beer to improve functional and sensory properties of this beverage.
Chemical analyses by UHPLC-QToF-MS revealed presence of: hidroxybenzoic acid derivatives (vanillic acid hexoside up to 141.476 mg/100g extract), hidroxycinnamic acid derivatives (caffeoylquinic acid isomer up to 306.608 mg/100g extract and feruloylquinic acid up to 225.884 mg/100g extract) and flavone derivatives (apigenin 180.094 mg/100g extract).
The evaluation of bioactive properties undoubtedly showed that both blackthorn extracts have moderate to rather good antimicrobial and antibiofilm potential, with ethanolic extract being slightly better in terms of inhibiting microbial growth. The best antimicrobial potential was observed with aqueous extract towards foodborne pathogenic bacteria Bacillus cereus (MIC 0.5 mg/mL, MBC 1.00 mg/mL), whereas other tested bacteria were more resilient towards the activity of the tested extracts (MIC in range of 1.0-4.0 mg/mL and MBC in range of 2.00-8.00 mg/mL). In terms of antifungal potential, the extracts showed rather uniform inhibitory effects towards tested microfungi with MIC value 1.00 mg/mL and MFC 2.00 mg/mL. A more in depth analysis of antimicrobial activity showed that the tested blackthorn extracts have promising antibiofilm activity towards Candida albicans ATCC 10231 as well, with both samples inhibiting formation of biofilm at subinhibitory levels approximately 67.00 %. Observed activity may be attributed to the presence of various phenolic compounds. 
Considering the well-known bitter taste of P. spinosa and demonstrated bioactive properties of its extracts, we believe it would be a great functional ingredient to add to the unique sensory properties of beer and similar beverages. This beer may even be able to provide consumers with additional health benefits, balancing enjoyment with the desire for healthy ingredients.
PB  - Novi Sad: Institute of Field and Vegetable Crops
C3  - Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia
T1  - Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima
T1  - Valorization of Prunus spinosa L. extracts as functional ingredients in alcoholic beverages
SP  - 24
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6391
ER  - 

@conference{
author = "Carević, Tamara and Petrović, Jovana and Ivanov, Marija and Milinčić, Danijel and Pešić, Mirjana and Ćirić, Ana",
year = "2023",
abstract = "Kontinuirani rast tržišta piva i alkoholnih pića otvorio je nove mogućnosti za poboljšanje ukusa, arome i korisnosti ovih napitaka. Već hiljadu godina unazad je poznato da dodavanje različitih biljnih sastojaka u piće menja njihov ukus i svojstva. Međutim, moderna tehnologija olakšava postizanje željenih karakteristika i nudi nove mogućnosti za terapeutske efekte i senzorna poboljšanja ovog tradicionalnog pića.
U ovom radu su predstavljene hemijska analiza i bioaktivna svojstva etanolnog i vodenog ekstrakta Prunus spinosa L. (trnjine) kao potencijalnog dodatka pivu, sa ciljem povećanja funkcionalnih karakteristika i senzornih osobina ovog napitka.
Hemijske analize korišćenjem UHPLC-QToF-MS pokazale su prisustvo: derivata hidroksibenzoeve kiseline (heksozid vanilinske kiseline do 141.476 mg/100 g ekstrakta), derivata hidroksicimetne kiseline (izomer kafeoilkvinske kiseline do 306.608 mg/100 g ekstrakta, feruloilkvinske kiseline do 225.884 mg/100 g ekstrakta) i derivata flavona (apigenin 180.094 mg/100g ekstrakta).
Procena bioaktivnih svojstava nesumnjivo je pokazala da oba ekstrakta trnjine imaju umeren do dobar antimikrobni potencijal, kao i sposobnost da inhibiraju formiranje biofilma, pri čemu je etanolni ekstrakt pokazao sveukupni bolji potencijal u inhibiciji rasta mikroorganizama. Najbolji antimikrobni potencijal je pokazan kod vodenog ekstrakta prema Bacillus cereus koja je poznati patogen identifikovan u namirnicama (MIC 0.5 mg/mL, MBC 1.00 mg/mL), dok su druge testirane bakterije bile otpornije na aktivnost testiranih ekstrakata (MIC u opsegu od 1.0- 4.0 mg/mL i MBC u opsegu od 2.00-8.00 mg/mL). Rezultati antifungalne aktivnosti su pokazali da ekstrakti prilično ujednačeno deluju inhibitorno na rast testiranih mikrogljiva, uz MIK vrednosti 1.00 mg/mL i MFK 2.00 mg/mL. Detaljnija analiza antimikrobne aktivnosti pokazala je da testirani ekstrakti trnjine značajno inhibiraju formiranje biofilma Candida albicans ATCC 10231; oba testirana uzorka su inhibirala formiranje biofilma na subinhibitornim koncentracijama približno 67.00 %. Uočena aktivnost se može pripisati prisustvu različitih fenolnih jedinjenja.
Imajući u vidu poznat gorak ukus P. spinosa kao i pokazana bioaktivna svojstva njegovih ekstrakata, verujemo da bi ova biljka bila odličan funkcionalni sastojak koji će se dobro uklopiti u jedinstvena senzorna svojstva piva i sličnih napitaka. Predloženo pivo bi čak moglo da pruži dodatne zdravstvene efekte potrošačima, balansirajući između zadovoljstva i njihove želje za zdravim sastojcima., The continuous growth of beer and beverage market has opened new opportunities to improve their flavor, aroma, and benefits. It has been known for thousands of years that adding various herbs to beer changes the taste and properties of this beverage. However, modern technology makes it easier to achieve the desired properties and provides useful qualities for therapeutic effects and sensory improvement of traditional beer. This paper presents chemical analysis and bioactive properties of ethanolic and aqueous extract of Prunus spinosa L. (blackthorn) as a potential ingredient for beer to improve functional and sensory properties of this beverage.
Chemical analyses by UHPLC-QToF-MS revealed presence of: hidroxybenzoic acid derivatives (vanillic acid hexoside up to 141.476 mg/100g extract), hidroxycinnamic acid derivatives (caffeoylquinic acid isomer up to 306.608 mg/100g extract and feruloylquinic acid up to 225.884 mg/100g extract) and flavone derivatives (apigenin 180.094 mg/100g extract).
The evaluation of bioactive properties undoubtedly showed that both blackthorn extracts have moderate to rather good antimicrobial and antibiofilm potential, with ethanolic extract being slightly better in terms of inhibiting microbial growth. The best antimicrobial potential was observed with aqueous extract towards foodborne pathogenic bacteria Bacillus cereus (MIC 0.5 mg/mL, MBC 1.00 mg/mL), whereas other tested bacteria were more resilient towards the activity of the tested extracts (MIC in range of 1.0-4.0 mg/mL and MBC in range of 2.00-8.00 mg/mL). In terms of antifungal potential, the extracts showed rather uniform inhibitory effects towards tested microfungi with MIC value 1.00 mg/mL and MFC 2.00 mg/mL. A more in depth analysis of antimicrobial activity showed that the tested blackthorn extracts have promising antibiofilm activity towards Candida albicans ATCC 10231 as well, with both samples inhibiting formation of biofilm at subinhibitory levels approximately 67.00 %. Observed activity may be attributed to the presence of various phenolic compounds. 
Considering the well-known bitter taste of P. spinosa and demonstrated bioactive properties of its extracts, we believe it would be a great functional ingredient to add to the unique sensory properties of beer and similar beverages. This beer may even be able to provide consumers with additional health benefits, balancing enjoyment with the desire for healthy ingredients.",
publisher = "Novi Sad: Institute of Field and Vegetable Crops",
journal = "Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia",
title = "Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima, Valorization of Prunus spinosa L. extracts as functional ingredients in alcoholic beverages",
pages = "24-27",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6391"
}

Carević, T., Petrović, J., Ivanov, M., Milinčić, D., Pešić, M.,& Ćirić, A.. (2023). Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima. in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia
Novi Sad: Institute of Field and Vegetable Crops., 24-27.
https://hdl.handle.net/21.15107/rcub_ibiss_6391

Carević T, Petrović J, Ivanov M, Milinčić D, Pešić M, Ćirić A. Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima. in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia. 2023;:24-27.
https://hdl.handle.net/21.15107/rcub_ibiss_6391 .

Carević, Tamara, Petrović, Jovana, Ivanov, Marija, Milinčić, Danijel, Pešić, Mirjana, Ćirić, Ana, "Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima" in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia (2023):24-27,
https://hdl.handle.net/21.15107/rcub_ibiss_6391 .

TY  - JOUR
AU  - Perić, Mirjana
AU  - Čairović, Aleksandra
AU  - Špadijer Gostović, Aleksandra
AU  - Stanimirović, Dragan
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Pavlović, Vladimir B.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6005
AB  - The purpose of this study was to evaluate the surface texture and biofilm adhe sion of veneered or CAD/CAM milled zirconia (partially stabilized with yttrium) after professional
oral hygiene procedures. The samples (4 × 4 mm, thickness 2 mm; n = 72) were separated from
zirconia blanks (3Y-TZP-LA). One group was veenered with ceramics, and the other group of samples
was CAD/CAM milled. Each group had two subgroups: polished and glazed. The samples were
subjected to simulated strokes of professional brushing using abrasive paste and ultrasonic scaling.
The parameters of surface micromorphology and receptivity to biofilm were calculated before and
after simulating the given methods of the professional maintenance of oral hygiene. Scanning electron
microscopy (SEM) was used to evaluate zirconia surface properties. Microbial (bacterial/fungal)
species (Staphylococcus aureus, Streptococcus sanguinis and Candida albicans) were used and cultured
on respective sterilized zirconia surfaces. Colony-forming unit (CFU) counts were used to quantify
the amount of biofilm formation on zirconia samples surfaces. Results: The SEM analysis showed
the greatest change in surface microtopography after the use of ultrasonic scaling on glazed zirconia
samples. Less formation of colonies on the surfaces of CAD/CAM milled zirconia restorations was
observed. Conclusion: Routine methods of oral hygiene professional maintenance can damage the
surfaces of glazed zirconia restorations.
PB  - Basel: MDPI
T2  - Applied Sciences
T1  - Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study
IS  - 14
VL  - 13
DO  - 10.3390/app13148389
SP  - 8389
ER  - 

@article{
author = "Perić, Mirjana and Čairović, Aleksandra and Špadijer Gostović, Aleksandra and Stanimirović, Dragan and Kostić, Marina and Ivanov, Marija and Ćirić, Ana and Glamočlija, Jasmina and Pavlović, Vladimir B.",
year = "2023",
abstract = "The purpose of this study was to evaluate the surface texture and biofilm adhe sion of veneered or CAD/CAM milled zirconia (partially stabilized with yttrium) after professional
oral hygiene procedures. The samples (4 × 4 mm, thickness 2 mm; n = 72) were separated from
zirconia blanks (3Y-TZP-LA). One group was veenered with ceramics, and the other group of samples
was CAD/CAM milled. Each group had two subgroups: polished and glazed. The samples were
subjected to simulated strokes of professional brushing using abrasive paste and ultrasonic scaling.
The parameters of surface micromorphology and receptivity to biofilm were calculated before and
after simulating the given methods of the professional maintenance of oral hygiene. Scanning electron
microscopy (SEM) was used to evaluate zirconia surface properties. Microbial (bacterial/fungal)
species (Staphylococcus aureus, Streptococcus sanguinis and Candida albicans) were used and cultured
on respective sterilized zirconia surfaces. Colony-forming unit (CFU) counts were used to quantify
the amount of biofilm formation on zirconia samples surfaces. Results: The SEM analysis showed
the greatest change in surface microtopography after the use of ultrasonic scaling on glazed zirconia
samples. Less formation of colonies on the surfaces of CAD/CAM milled zirconia restorations was
observed. Conclusion: Routine methods of oral hygiene professional maintenance can damage the
surfaces of glazed zirconia restorations.",
publisher = "Basel: MDPI",
journal = "Applied Sciences",
title = "Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study",
number = "14",
volume = "13",
doi = "10.3390/app13148389",
pages = "8389"
}

Perić, M., Čairović, A., Špadijer Gostović, A., Stanimirović, D., Kostić, M., Ivanov, M., Ćirić, A., Glamočlija, J.,& Pavlović, V. B.. (2023). Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study. in Applied Sciences
Basel: MDPI., 13(14), 8389.
https://doi.org/10.3390/app13148389

Perić M, Čairović A, Špadijer Gostović A, Stanimirović D, Kostić M, Ivanov M, Ćirić A, Glamočlija J, Pavlović VB. Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study. in Applied Sciences. 2023;13(14):8389.
doi:10.3390/app13148389 .

Perić, Mirjana, Čairović, Aleksandra, Špadijer Gostović, Aleksandra, Stanimirović, Dragan, Kostić, Marina, Ivanov, Marija, Ćirić, Ana, Glamočlija, Jasmina, Pavlović, Vladimir B., "Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study" in Applied Sciences, 13, no. 14 (2023):8389,
https://doi.org/10.3390/app13148389 . .

TY  - JOUR
AU  - Perović, Tatjana
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Kostić, Marina
AU  - Ćirić, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5498
AB  - Plant extracts and other plant products have been used as an alternative to synthetic fungicides or an additional way to reduce their use. The choice of plant extracts and their application depends on their functional characteristics, availability, cost-effectiveness and their impact on phytopathogens, and also on the environment. Therefore, the present study aims to assess the potential of Celtis australis methanolic extracts as source of compounds with antifungal activity. Methanolic extracts prepared from leaves and unripe mesocarps of C. australis collected from different localities of Montenegro (Podgorica-PG, Donja Gorica-DG, and Bar-BR), were evaluated for their phenolic compounds composition as well as antifungal and cytotoxic properties. Obtained results revealed that extracts contain various bioactive constituents including phenolic acids, flavonoids and their derivatives. The predominant phenolic acid was ferulic acid, identified in leaves samples from DG (187.97 mg/100g dw), while isoorientin was the most abundant phenolic compound found in all examined samples. Regarding antifungal potential of the tested samples, all but one (prepared from mesocarp BR) possessed higher activity, than Previcur, commercial systemic fungicide intended to control seedling. In vitro studies on HaCaT cell line showed that the extracts had no toxic effect towards the tested cell line. These results lead to the conclusion that methanolic extracts of C. australis can become an alternative to the use of synthetic fungicides in agriculture. Those extracts represent natural biodegradable fungicides and enable a more efficient control of pathogenic fungi.
PB  - John Wiley and Sons Inc
T2  - Food Science and Nutrition
T1  - Natural extracts against agricultural pathogens: A case study of Celtis australis L.
DO  - 10.1002/fsn3.3325
ER  - 

@article{
author = "Perović, Tatjana and Petrović, Jovana and Gašić, Uroš and Kostić, Marina and Ćirić, Ana",
year = "2023",
abstract = "Plant extracts and other plant products have been used as an alternative to synthetic fungicides or an additional way to reduce their use. The choice of plant extracts and their application depends on their functional characteristics, availability, cost-effectiveness and their impact on phytopathogens, and also on the environment. Therefore, the present study aims to assess the potential of Celtis australis methanolic extracts as source of compounds with antifungal activity. Methanolic extracts prepared from leaves and unripe mesocarps of C. australis collected from different localities of Montenegro (Podgorica-PG, Donja Gorica-DG, and Bar-BR), were evaluated for their phenolic compounds composition as well as antifungal and cytotoxic properties. Obtained results revealed that extracts contain various bioactive constituents including phenolic acids, flavonoids and their derivatives. The predominant phenolic acid was ferulic acid, identified in leaves samples from DG (187.97 mg/100g dw), while isoorientin was the most abundant phenolic compound found in all examined samples. Regarding antifungal potential of the tested samples, all but one (prepared from mesocarp BR) possessed higher activity, than Previcur, commercial systemic fungicide intended to control seedling. In vitro studies on HaCaT cell line showed that the extracts had no toxic effect towards the tested cell line. These results lead to the conclusion that methanolic extracts of C. australis can become an alternative to the use of synthetic fungicides in agriculture. Those extracts represent natural biodegradable fungicides and enable a more efficient control of pathogenic fungi.",
publisher = "John Wiley and Sons Inc",
journal = "Food Science and Nutrition",
title = "Natural extracts against agricultural pathogens: A case study of Celtis australis L.",
doi = "10.1002/fsn3.3325"
}

Perović, T., Petrović, J., Gašić, U., Kostić, M.,& Ćirić, A.. (2023). Natural extracts against agricultural pathogens: A case study of Celtis australis L.. in Food Science and Nutrition
John Wiley and Sons Inc..
https://doi.org/10.1002/fsn3.3325

Perović T, Petrović J, Gašić U, Kostić M, Ćirić A. Natural extracts against agricultural pathogens: A case study of Celtis australis L.. in Food Science and Nutrition. 2023;.
doi:10.1002/fsn3.3325 .

Perović, Tatjana, Petrović, Jovana, Gašić, Uroš, Kostić, Marina, Ćirić, Ana, "Natural extracts against agricultural pathogens: A case study of Celtis australis L." in Food Science and Nutrition (2023),
https://doi.org/10.1002/fsn3.3325 . .

TY  - JOUR
AU  - Barbakadze, Vakhtang
AU  - Merlani, Maia
AU  - Gogilashvili, Lali
AU  - Amiranashvili, Lela
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2023
UR  - https://www.mdpi.com/2079-6382/12/2/285
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36830198
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9952037
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5491
AB  - This study reports the antimicrobial activities of the biopolymers poly[3-(3,4-dihydoxyphenyl)glyceric acid] (PDHPGA) and poly[2-methoxycarbonyl-3-(3,4-dihydroxyphenyl)oxirane] (PMDHPO), extracted from the six plants of Boraginaceae family: Symphytum asperum (SA), S. caucasicum (SC), S. gr and iflorum (SG), Anchusa italica (AI), Cynoglosum officinale (CO), and Borago officinalis (BO) collected in various parts of Georgia. The study revealed that the antibacterial activities were moderate, and biopolymers from only three plants showed activities against all tested bacteria. Biopolymers from CO stems as well as SC and AI did not show any activity except low activity against a resistant P. aeruginosa strain, which was the most resistant among all three resistant strains. On the other hand, the antifungal activity was better compared to the antibacterial activity. Biopolymers from BO stems exhibited the best activities with MIC/MFC at 0.37-1.00 mg/mL and 0.75-1.5 mg/L, respectively, followed by those from SG stems. Biopolymers from SC and AI roots showed antifungal activities against all six fungi, in contrast to the antibacterial activity, while biopolymers from CO stems and SA roots had activities against four fungi and one fungus, respectively. The sugar-based catechol-containing biopolymers from BO stems demonstrated the best activities among all tested biopolymers against T. viride, P. funiculosum, P. cyclpoium var verucosum, and C. albicans (MIC 0.37 mg/mL). In addition, biopolymers from SG stems were half as active against A. fumigatus and T. viride as ketoconazole. Biopolymers from all plant materials except for CO stems showed higher potency than ketoconazole against T. viride. For the first time, it was shown that all plant materials exhibited better activity against C. albicans, one of the most dreadful fungal species.
PB  - Basel: MDPI
T2  - Antibiotics (Basel, Switzerland)
T1  - Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family
IS  - 2
VL  - 12
DO  - 10.3390/antibiotics12020285
SP  - 285
ER  - 

@article{
author = "Barbakadze, Vakhtang and Merlani, Maia and Gogilashvili, Lali and Amiranashvili, Lela and Petrou, Anthi and Geronikaki, Athina and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2023",
abstract = "This study reports the antimicrobial activities of the biopolymers poly[3-(3,4-dihydoxyphenyl)glyceric acid] (PDHPGA) and poly[2-methoxycarbonyl-3-(3,4-dihydroxyphenyl)oxirane] (PMDHPO), extracted from the six plants of Boraginaceae family: Symphytum asperum (SA), S. caucasicum (SC), S. gr and iflorum (SG), Anchusa italica (AI), Cynoglosum officinale (CO), and Borago officinalis (BO) collected in various parts of Georgia. The study revealed that the antibacterial activities were moderate, and biopolymers from only three plants showed activities against all tested bacteria. Biopolymers from CO stems as well as SC and AI did not show any activity except low activity against a resistant P. aeruginosa strain, which was the most resistant among all three resistant strains. On the other hand, the antifungal activity was better compared to the antibacterial activity. Biopolymers from BO stems exhibited the best activities with MIC/MFC at 0.37-1.00 mg/mL and 0.75-1.5 mg/L, respectively, followed by those from SG stems. Biopolymers from SC and AI roots showed antifungal activities against all six fungi, in contrast to the antibacterial activity, while biopolymers from CO stems and SA roots had activities against four fungi and one fungus, respectively. The sugar-based catechol-containing biopolymers from BO stems demonstrated the best activities among all tested biopolymers against T. viride, P. funiculosum, P. cyclpoium var verucosum, and C. albicans (MIC 0.37 mg/mL). In addition, biopolymers from SG stems were half as active against A. fumigatus and T. viride as ketoconazole. Biopolymers from all plant materials except for CO stems showed higher potency than ketoconazole against T. viride. For the first time, it was shown that all plant materials exhibited better activity against C. albicans, one of the most dreadful fungal species.",
publisher = "Basel: MDPI",
journal = "Antibiotics (Basel, Switzerland)",
title = "Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family",
number = "2",
volume = "12",
doi = "10.3390/antibiotics12020285",
pages = "285"
}

Barbakadze, V., Merlani, M., Gogilashvili, L., Amiranashvili, L., Petrou, A., Geronikaki, A., Ćirić, A., Glamočlija, J.,& Soković, M.. (2023). Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family. in Antibiotics (Basel, Switzerland)
Basel: MDPI., 12(2), 285.
https://doi.org/10.3390/antibiotics12020285

Barbakadze V, Merlani M, Gogilashvili L, Amiranashvili L, Petrou A, Geronikaki A, Ćirić A, Glamočlija J, Soković M. Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family. in Antibiotics (Basel, Switzerland). 2023;12(2):285.
doi:10.3390/antibiotics12020285 .

Barbakadze, Vakhtang, Merlani, Maia, Gogilashvili, Lali, Amiranashvili, Lela, Petrou, Anthi, Geronikaki, Athina, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family" in Antibiotics (Basel, Switzerland), 12, no. 2 (2023):285,
https://doi.org/10.3390/antibiotics12020285 . .

TY  - JOUR
AU  - Đorđevski, Nikoleta
AU  - Uba, Abdullahi Ibrahim
AU  - Zengin, Gokhan
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Ristanović, Elizabeta
AU  - Ćirić, Ana
AU  - Nikolić, Biljana
AU  - Stojković, Dejan
PY  - 2023
UR  - https://www.mdpi.com/1424-8247/16/1/21
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9865742
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5433
AB  - This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of Allium scorodoprasum L. (Amaryllidaceae)-the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(-)HESI-QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3-O-glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of A. scorodoprasum with contemporary in vitro scientific approaches.
PB  - Basel: MDPI
T2  - Pharmaceuticals (Basel, Switzerland)
T1  - Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts
IS  - 1
VL  - 16
DO  - 10.3390/ph16010021
SP  - 21
ER  - 

@article{
author = "Đorđevski, Nikoleta and Uba, Abdullahi Ibrahim and Zengin, Gokhan and Božunović, Jelena and Gašić, Uroš and Ristanović, Elizabeta and Ćirić, Ana and Nikolić, Biljana and Stojković, Dejan",
year = "2023",
abstract = "This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of Allium scorodoprasum L. (Amaryllidaceae)-the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(-)HESI-QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3-O-glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of A. scorodoprasum with contemporary in vitro scientific approaches.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals (Basel, Switzerland)",
title = "Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts",
number = "1",
volume = "16",
doi = "10.3390/ph16010021",
pages = "21"
}

Đorđevski, N., Uba, A. I., Zengin, G., Božunović, J., Gašić, U., Ristanović, E., Ćirić, A., Nikolić, B.,& Stojković, D.. (2023). Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts. in Pharmaceuticals (Basel, Switzerland)
Basel: MDPI., 16(1), 21.
https://doi.org/10.3390/ph16010021

Đorđevski N, Uba AI, Zengin G, Božunović J, Gašić U, Ristanović E, Ćirić A, Nikolić B, Stojković D. Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts. in Pharmaceuticals (Basel, Switzerland). 2023;16(1):21.
doi:10.3390/ph16010021 .

Đorđevski, Nikoleta, Uba, Abdullahi Ibrahim, Zengin, Gokhan, Božunović, Jelena, Gašić, Uroš, Ristanović, Elizabeta, Ćirić, Ana, Nikolić, Biljana, Stojković, Dejan, "Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts" in Pharmaceuticals (Basel, Switzerland), 16, no. 1 (2023):21,
https://doi.org/10.3390/ph16010021 . .

TY  - CONF
AU  - Stojković, Dejan
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Petrović, Jovana
AU  - Kostić, Marina
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5158
AB  - Chicory (Cichorium intybus L.) is an important industrial crop cultivated mainly to extract the
dietary fiber inulin. This study aimed to investigate antimicrobial, antibiofilm and wound healing
potentials of the extract obtained by supercritical fluid extraction (SFE). Antibacterial activity
of extract ranged from 0.25 to 4.00 mg/mL regarding minimal inhibitory concentra-tions (MICs),
while minimal bactericidal concentrations (MBCs) were noted within the range of 0.5 to 8.00 mg/
mL. The extract has shown promising antifungal potential with MICs rang-ing 0.125- 0.75 mg/
mL and MFCs 0.25-1.0 mg/mL against Candida species. Incubation with the extract has reduced
the ability of both C. albicans and P. aeruginosa to attach to the sur-face and begin the process
of biofilm formation. Concentration equal to previously deter-mined MIC has almost completely
(>90% inhibition) reduced the biofilm biomass formed by P. aeruginosa. Even twice as lower concentrations
(0.5 MIC) has limited the biofilm forming ability and induce more than 80% inhibition
in P. aeruginosa as bacterial biofilm model or-ganism. Impact on fungal biofilm was less profound
and MIC concentration was able to re-duce the biofilm formation for less than 60%. HaCaT (human
immortalized keratinocytes cell line) cells were treated with IC20 (24 h) concentration of chicory
SFE extract. It is estimated that SFE extract has slight effect on wound closure in keratinocyte
monolayer, when com-pared to untreated control. Since, Chicory SFE fraction possessed excellent
antibacterial po-tential against E. coli and K. pneumoniae and antifungal activity against Candida
species these results are considered very important in the future inventions
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5158
ER  - 

@conference{
author = "Stojković, Dejan and Ivanov, Marija and Ćirić, Ana and Petrović, Jovana and Kostić, Marina and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Chicory (Cichorium intybus L.) is an important industrial crop cultivated mainly to extract the
dietary fiber inulin. This study aimed to investigate antimicrobial, antibiofilm and wound healing
potentials of the extract obtained by supercritical fluid extraction (SFE). Antibacterial activity
of extract ranged from 0.25 to 4.00 mg/mL regarding minimal inhibitory concentra-tions (MICs),
while minimal bactericidal concentrations (MBCs) were noted within the range of 0.5 to 8.00 mg/
mL. The extract has shown promising antifungal potential with MICs rang-ing 0.125- 0.75 mg/
mL and MFCs 0.25-1.0 mg/mL against Candida species. Incubation with the extract has reduced
the ability of both C. albicans and P. aeruginosa to attach to the sur-face and begin the process
of biofilm formation. Concentration equal to previously deter-mined MIC has almost completely
(>90% inhibition) reduced the biofilm biomass formed by P. aeruginosa. Even twice as lower concentrations
(0.5 MIC) has limited the biofilm forming ability and induce more than 80% inhibition
in P. aeruginosa as bacterial biofilm model or-ganism. Impact on fungal biofilm was less profound
and MIC concentration was able to re-duce the biofilm formation for less than 60%. HaCaT (human
immortalized keratinocytes cell line) cells were treated with IC20 (24 h) concentration of chicory
SFE extract. It is estimated that SFE extract has slight effect on wound closure in keratinocyte
monolayer, when com-pared to untreated control. Since, Chicory SFE fraction possessed excellent
antibacterial po-tential against E. coli and K. pneumoniae and antifungal activity against Candida
species these results are considered very important in the future inventions",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5158"
}

Stojković, D., Ivanov, M., Ćirić, A., Petrović, J., Kostić, M., Glamočlija, J.,& Soković, M.. (2022). Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5158

Stojković D, Ivanov M, Ćirić A, Petrović J, Kostić M, Glamočlija J, Soković M. Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5158 .

Stojković, Dejan, Ivanov, Marija, Ćirić, Ana, Petrović, Jovana, Kostić, Marina, Glamočlija, Jasmina, Soković, Marina, "Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5158 .

TY  - CONF
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Kostić, Marina
AU  - Petrović, Jovana
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5581
AB  - Cichorium intybus L. (Asteraceae), is an important medicinal plant, rich in sesquiterpene
lactones (STLs), inulin, biologically relevant elements, and vitamins which exert potent
pro-health effects on the human organism. The aim of this study was to screen two
extracts of chicory containing STLs, the optimized extract (Ci_SFEopt) and the
supercritical fluid extract (Ci_SFEpur), for their antimicrobial activities against a range
of pathogenic microorganisms. Both extracts showed antibacterial activity, with
Ci_SFEpur being more potent than Ci_SFEopt. The most sensitive species was
Pseudomonas aeruginosa (minimal inhibitory concentration, MIC 0.06 mg/mL).
Ci_SFEpur and Ci_SFEopt extracts demonstrated good antifungal activity against
Candida species (MIC 0.03-1.00 mg/mL). Thin layer chromatography (TLC)
bioautography was used to screen the extracts and provide basic information for the
isolation of bioactive compounds in the future. Ci_SFEopt was active against all tested
microorganisms (Rf values were 0.18-0.88). The Ci_SFEpur extract efficiently inhibited
(Rf 0.76) Staphylococcus aureus, P. aeruginosa and Candida krusei. The identification
of powerful antimicrobial activity of Ci_SFEpur against P. aeruginosa and Candida
species is an important step towards the development of novel antimicrobial therapeutics
and efficient utilization of this widely distributed plant.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Evaluation of antimicrobial properties of chicory extracts
SP  - 257
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5581
ER  - 

@conference{
author = "Ćirić, Ana and Ivanov, Marija and Stojković, Dejan and Glamočlija, Jasmina and Kostić, Marina and Petrović, Jovana and Soković, Marina",
year = "2022",
abstract = "Cichorium intybus L. (Asteraceae), is an important medicinal plant, rich in sesquiterpene
lactones (STLs), inulin, biologically relevant elements, and vitamins which exert potent
pro-health effects on the human organism. The aim of this study was to screen two
extracts of chicory containing STLs, the optimized extract (Ci_SFEopt) and the
supercritical fluid extract (Ci_SFEpur), for their antimicrobial activities against a range
of pathogenic microorganisms. Both extracts showed antibacterial activity, with
Ci_SFEpur being more potent than Ci_SFEopt. The most sensitive species was
Pseudomonas aeruginosa (minimal inhibitory concentration, MIC 0.06 mg/mL).
Ci_SFEpur and Ci_SFEopt extracts demonstrated good antifungal activity against
Candida species (MIC 0.03-1.00 mg/mL). Thin layer chromatography (TLC)
bioautography was used to screen the extracts and provide basic information for the
isolation of bioactive compounds in the future. Ci_SFEopt was active against all tested
microorganisms (Rf values were 0.18-0.88). The Ci_SFEpur extract efficiently inhibited
(Rf 0.76) Staphylococcus aureus, P. aeruginosa and Candida krusei. The identification
of powerful antimicrobial activity of Ci_SFEpur against P. aeruginosa and Candida
species is an important step towards the development of novel antimicrobial therapeutics
and efficient utilization of this widely distributed plant.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Evaluation of antimicrobial properties of chicory extracts",
pages = "257",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5581"
}

Ćirić, A., Ivanov, M., Stojković, D., Glamočlija, J., Kostić, M., Petrović, J.,& Soković, M.. (2022). Evaluation of antimicrobial properties of chicory extracts. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 257.
https://hdl.handle.net/21.15107/rcub_ibiss_5581

Ćirić A, Ivanov M, Stojković D, Glamočlija J, Kostić M, Petrović J, Soković M. Evaluation of antimicrobial properties of chicory extracts. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:257.
https://hdl.handle.net/21.15107/rcub_ibiss_5581 .

Ćirić, Ana, Ivanov, Marija, Stojković, Dejan, Glamočlija, Jasmina, Kostić, Marina, Petrović, Jovana, Soković, Marina, "Evaluation of antimicrobial properties of chicory extracts" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):257,
https://hdl.handle.net/21.15107/rcub_ibiss_5581 .

TY  - CONF
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Caleja, Cristina
AU  - Pereira, Eliana
AU  - Barros, Lillian
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5156
AB  - Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Chemical composition and biological activity of two Lactarius species from Serbia.
SP  - 102
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5156
ER  - 

@conference{
author = "Kostić, Marina and Ivanov, Marija and Ćirić, Ana and Caleja, Cristina and Pereira, Eliana and Barros, Lillian and Stojković, Dejan and Soković, Marina and Glamočlija, Jasmina",
year = "2022",
abstract = "Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Chemical composition and biological activity of two Lactarius species from Serbia.",
pages = "102-103",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5156"
}

Kostić, M., Ivanov, M., Ćirić, A., Caleja, C., Pereira, E., Barros, L., Stojković, D., Soković, M.,& Glamočlija, J.. (2022). Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156

Kostić M, Ivanov M, Ćirić A, Caleja C, Pereira E, Barros L, Stojković D, Soković M, Glamočlija J. Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

Kostić, Marina, Ivanov, Marija, Ćirić, Ana, Caleja, Cristina, Pereira, Eliana, Barros, Lillian, Stojković, Dejan, Soković, Marina, Glamočlija, Jasmina, "Chemical composition and biological activity of two Lactarius species from Serbia." in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):102-103,
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Sanković Babić, Snežana
AU  - Tepavčević, Zvezdana
AU  - Radanović, Oliver
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2022
UR  - https://www.mdpi.com/2079-6382/11/12/1747
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5353
AB  - Chronic tonsillitis (CT) is a global health issue which can impair patient’s quality of life and has an important socioeconomic impact due to the nonrational use of antibiotics, increased antimicrobial resistance and frequent need for surgical treatment. In order to isolate and identify the causing agents of CT, a total of 79 postoperative palatine and adenoid tissue samples were obtained from the ENT Clinic, KBC Zvezdara, Belgrade, Serbia. Culture identification was performed by MALDI-TOF MS and the Staphylococcus aureus isolates were tested for biofilm forming capability and antibiotic susceptibility. Additionally, a histological examination of palatine and adenoid tissue was performed in order to detect the presence of CT-causing bacteria. The slight majority of participants were females with median age of 28 years for adult patients (group I) and 6 years for children (group II). Analysis of the incidence of bacteria isolated from tissue samples in both groups showed the highest prevalence of S. aureus, Streptococcus oralis and Streptococcus parasanquinis. In addition to interfollicular hyperplasia, colonies of species S. aureus were detected in histological material. The presence of biofilm might be the reason for the recurrence of infection. Therefore, searching for a new treatment of CT is of great importance.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Analysis of Tonsil Tissues from Patients Diagnosed with Chronic Tonsillitis—Microbiological Profile, Biofilm-Forming Capacity and Histology
IS  - 12
VL  - 11
DO  - 10.3390/antibiotics11121747
SP  - 1747
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Sanković Babić, Snežana and Tepavčević, Zvezdana and Radanović, Oliver and Soković, Marina and Ćirić, Ana",
year = "2022",
abstract = "Chronic tonsillitis (CT) is a global health issue which can impair patient’s quality of life and has an important socioeconomic impact due to the nonrational use of antibiotics, increased antimicrobial resistance and frequent need for surgical treatment. In order to isolate and identify the causing agents of CT, a total of 79 postoperative palatine and adenoid tissue samples were obtained from the ENT Clinic, KBC Zvezdara, Belgrade, Serbia. Culture identification was performed by MALDI-TOF MS and the Staphylococcus aureus isolates were tested for biofilm forming capability and antibiotic susceptibility. Additionally, a histological examination of palatine and adenoid tissue was performed in order to detect the presence of CT-causing bacteria. The slight majority of participants were females with median age of 28 years for adult patients (group I) and 6 years for children (group II). Analysis of the incidence of bacteria isolated from tissue samples in both groups showed the highest prevalence of S. aureus, Streptococcus oralis and Streptococcus parasanquinis. In addition to interfollicular hyperplasia, colonies of species S. aureus were detected in histological material. The presence of biofilm might be the reason for the recurrence of infection. Therefore, searching for a new treatment of CT is of great importance.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Analysis of Tonsil Tissues from Patients Diagnosed with Chronic Tonsillitis—Microbiological Profile, Biofilm-Forming Capacity and Histology",
number = "12",
volume = "11",
doi = "10.3390/antibiotics11121747",
pages = "1747"
}

Kostić, M., Ivanov, M., Sanković Babić, S., Tepavčević, Z., Radanović, O., Soković, M.,& Ćirić, A.. (2022). Analysis of Tonsil Tissues from Patients Diagnosed with Chronic Tonsillitis—Microbiological Profile, Biofilm-Forming Capacity and Histology. in Antibiotics
Basel: MDPI., 11(12), 1747.
https://doi.org/10.3390/antibiotics11121747

Kostić M, Ivanov M, Sanković Babić S, Tepavčević Z, Radanović O, Soković M, Ćirić A. Analysis of Tonsil Tissues from Patients Diagnosed with Chronic Tonsillitis—Microbiological Profile, Biofilm-Forming Capacity and Histology. in Antibiotics. 2022;11(12):1747.
doi:10.3390/antibiotics11121747 .

Kostić, Marina, Ivanov, Marija, Sanković Babić, Snežana, Tepavčević, Zvezdana, Radanović, Oliver, Soković, Marina, Ćirić, Ana, "Analysis of Tonsil Tissues from Patients Diagnosed with Chronic Tonsillitis—Microbiological Profile, Biofilm-Forming Capacity and Histology" in Antibiotics, 11, no. 12 (2022):1747,
https://doi.org/10.3390/antibiotics11121747 . .

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Marković, Tatjana
AU  - Sanković Babić, Snežana
AU  - Barros, Lillian
AU  - Calhelha, Ricardo
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/10412905.2022.2107103
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5101
AB  - The chemical composition and biological activity of essential oils (EOs) from leaves of Origanum minutiflorum O. Schwarz & P.H.Davis, and seeds of Coriandrum sativum L. were investigated. Chemical analyses of EOs were performed and the major components were carvacrol (81.5%) and linalool (69.6%), respectively. The antimicrobial activity was assessed against several bacteria originating from the tonsillar tissue. Activities of EOs against Staphylococcus aureus biofilm were investigated, as well as the effect of the mixture of these EOs and antibiotics against the pathogen. The antioxidant activity of both EOs was determined by TBARS assay, and examined wild oregano EO showed better activity. Also, cytotoxicity of EOs was evaluated in vitro and both EOs showed potential to inhibit further proliferation of tumor cells. This study reported for the first time the effects of EOs on chronic tonsillitis causative pathogens, supporting their role as a natural bioactive therapeutic agent with possible antimicrobial applications.
PB  - Taylor and Francis Ltd.
T2  - Journal of Essential Oil Research
T1  - An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities
IS  - 6
VL  - 34
DO  - 10.1080/10412905.2022.2107103
SP  - 533
EP  - 543
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Marković, Tatjana and Sanković Babić, Snežana and Barros, Lillian and Calhelha, Ricardo and Soković, Marina and Ćirić, Ana",
year = "2022",
abstract = "The chemical composition and biological activity of essential oils (EOs) from leaves of Origanum minutiflorum O. Schwarz & P.H.Davis, and seeds of Coriandrum sativum L. were investigated. Chemical analyses of EOs were performed and the major components were carvacrol (81.5%) and linalool (69.6%), respectively. The antimicrobial activity was assessed against several bacteria originating from the tonsillar tissue. Activities of EOs against Staphylococcus aureus biofilm were investigated, as well as the effect of the mixture of these EOs and antibiotics against the pathogen. The antioxidant activity of both EOs was determined by TBARS assay, and examined wild oregano EO showed better activity. Also, cytotoxicity of EOs was evaluated in vitro and both EOs showed potential to inhibit further proliferation of tumor cells. This study reported for the first time the effects of EOs on chronic tonsillitis causative pathogens, supporting their role as a natural bioactive therapeutic agent with possible antimicrobial applications.",
publisher = "Taylor and Francis Ltd.",
journal = "Journal of Essential Oil Research",
title = "An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities",
number = "6",
volume = "34",
doi = "10.1080/10412905.2022.2107103",
pages = "533-543"
}

Kostić, M., Ivanov, M., Marković, T., Sanković Babić, S., Barros, L., Calhelha, R., Soković, M.,& Ćirić, A.. (2022). An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities. in Journal of Essential Oil Research
Taylor and Francis Ltd.., 34(6), 533-543.
https://doi.org/10.1080/10412905.2022.2107103

Kostić M, Ivanov M, Marković T, Sanković Babić S, Barros L, Calhelha R, Soković M, Ćirić A. An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities. in Journal of Essential Oil Research. 2022;34(6):533-543.
doi:10.1080/10412905.2022.2107103 .

Kostić, Marina, Ivanov, Marija, Marković, Tatjana, Sanković Babić, Snežana, Barros, Lillian, Calhelha, Ricardo, Soković, Marina, Ćirić, Ana, "An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities" in Journal of Essential Oil Research, 34, no. 6 (2022):533-543,
https://doi.org/10.1080/10412905.2022.2107103 . .

TY  - CONF
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Sanković Babić, Snežana
AU  - Tapavčević, Zvezdana
AU  - Radanović, Oliver
AU  - Ćirić, Ana
AU  - Soković, Marina
PY  - 2022
UR  - https://www.femsbelgrade2022.org/abstract-book
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5155
AB  - If chronic tonsillitis (CT) is treated surgically, the long-term risks of infections and respiratory diseases are
higher, making of high importance to find an alternative for tonsillectomy. In order to isolate and identify
the causing agents of CT, a total of 79 postoperative palatine and adenoid tissue samples were obtained
from the ENT Clinic, KBC Zvezdara. A total of 82 bacterial species were isolated and identified by MALDI-TOF.
They belong to the genera Streptococcus, Staphylococcus, Micrococcus, Rothia, Enterobacter and Stenotrophomonas.
Analysis of the incidence of bacteria isolated from tissue samples showed the highest prevalence of Streptococcus
oralis (27/33%), followed by S. aureus (17/21%) and Streptococcus parasanquinis (16/20%). Histological analysis
of tissue samples was conducted in order to detect the presence of CT-causing bacteria. In addition to interfollicular
hyperplasia, colonies of species S. aureus and Stenotrophomonas maltophilia were detected in histological material.
The presented results confirm that the colonizers of tonsils tissues can be both G (+) and G (-) bacteria. The
presence of S. aureus in the tonsillar tissue even after the antibiotic therapy may be related to its ability to
form biofilm. Our study demonstrated that among 17 S. aureus isolates, 13 displays more pronounced biofilm
forming ability than the reference strain S. aureus ATCC 11632, and only 4 isolates display lower biofilm forming ability.
The presence of biofilm might be the reason for the recurrence of infection. Therefore, a searching for new
antibiofilm therapeutics in CT therapy is of great importance.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis
SP  - 853
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5155
ER  - 

@conference{
author = "Kostić, Marina and Ivanov, Marija and Sanković Babić, Snežana and Tapavčević, Zvezdana and Radanović, Oliver and Ćirić, Ana and Soković, Marina",
year = "2022",
abstract = "If chronic tonsillitis (CT) is treated surgically, the long-term risks of infections and respiratory diseases are
higher, making of high importance to find an alternative for tonsillectomy. In order to isolate and identify
the causing agents of CT, a total of 79 postoperative palatine and adenoid tissue samples were obtained
from the ENT Clinic, KBC Zvezdara. A total of 82 bacterial species were isolated and identified by MALDI-TOF.
They belong to the genera Streptococcus, Staphylococcus, Micrococcus, Rothia, Enterobacter and Stenotrophomonas.
Analysis of the incidence of bacteria isolated from tissue samples showed the highest prevalence of Streptococcus
oralis (27/33%), followed by S. aureus (17/21%) and Streptococcus parasanquinis (16/20%). Histological analysis
of tissue samples was conducted in order to detect the presence of CT-causing bacteria. In addition to interfollicular
hyperplasia, colonies of species S. aureus and Stenotrophomonas maltophilia were detected in histological material.
The presented results confirm that the colonizers of tonsils tissues can be both G (+) and G (-) bacteria. The
presence of S. aureus in the tonsillar tissue even after the antibiotic therapy may be related to its ability to
form biofilm. Our study demonstrated that among 17 S. aureus isolates, 13 displays more pronounced biofilm
forming ability than the reference strain S. aureus ATCC 11632, and only 4 isolates display lower biofilm forming ability.
The presence of biofilm might be the reason for the recurrence of infection. Therefore, a searching for new
antibiofilm therapeutics in CT therapy is of great importance.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis",
pages = "853",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5155"
}

Kostić, M., Ivanov, M., Sanković Babić, S., Tapavčević, Z., Radanović, O., Ćirić, A.,& Soković, M.. (2022). Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 853.
https://hdl.handle.net/21.15107/rcub_ibiss_5155

Kostić M, Ivanov M, Sanković Babić S, Tapavčević Z, Radanović O, Ćirić A, Soković M. Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:853.
https://hdl.handle.net/21.15107/rcub_ibiss_5155 .

Kostić, Marina, Ivanov, Marija, Sanković Babić, Snežana, Tapavčević, Zvezdana, Radanović, Oliver, Ćirić, Ana, Soković, Marina, "Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):853,
https://hdl.handle.net/21.15107/rcub_ibiss_5155 .

TY  - JOUR
AU  - Pogrebnoi, Serghei
AU  - Radul, Oleg
AU  - Stingaci, Eugenia
AU  - Lupascu, Lucian
AU  - Valica, Vladimir
AU  - Uncu, Livia
AU  - Smetanscaia, Anastasia
AU  - Petrou, Anthi
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Geronikaki, Athina
AU  - Macaev, Fliur Z.
PY  - 2022
UR  - https://www.mdpi.com/2079-6382/11/5/588
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4982
AB  - The control of fungal pathogens is increasingly difficult due to the limited number of effective drugs available for antifungal therapy. In addition, both humans and fungi are eukaryotic organisms; antifungal drugs may have significant toxicity due to the inhibition of related human targets. Furthermore, another problem is increased incidents of fungal resistance to azoles, such as fluconazole, ketoconazole, voriconazole, etc. Thus, the interest in developing new azoles with an extended spectrum of activity still attracts the interest of the scientific community. Herein, we report the synthesis of a series of triazolium salts, an evaluation of their antifungal activity, and docking studies. Ketoconazole and bifonazole were used as reference drugs. All compounds showed good antifungal activity with MIC/MFC in the range of 0.0003 to 0.2/0.0006-0.4 mg/mL. Compound 19 exhibited the best activity among all tested with MIC/MFC in the range of 0.009 to 0.037 mg/mL and 0.0125-0.05 mg/mL, respectively. All compounds appeared to be more potent than both reference drugs. The docking studies are in accordance with experimental results.
PB  - Basel: MDPI
T2  - Antibiotics (Basel, Switzerland)
T1  - The Synthesis of Triazolium Salts as Antifungal Agents: A Biological and In Silico Evaluation.
IS  - 5
VL  - 11
DO  - 10.3390/antibiotics11050588
SP  - 588
ER  - 

@article{
author = "Pogrebnoi, Serghei and Radul, Oleg and Stingaci, Eugenia and Lupascu, Lucian and Valica, Vladimir and Uncu, Livia and Smetanscaia, Anastasia and Petrou, Anthi and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina and Geronikaki, Athina and Macaev, Fliur Z.",
year = "2022",
abstract = "The control of fungal pathogens is increasingly difficult due to the limited number of effective drugs available for antifungal therapy. In addition, both humans and fungi are eukaryotic organisms; antifungal drugs may have significant toxicity due to the inhibition of related human targets. Furthermore, another problem is increased incidents of fungal resistance to azoles, such as fluconazole, ketoconazole, voriconazole, etc. Thus, the interest in developing new azoles with an extended spectrum of activity still attracts the interest of the scientific community. Herein, we report the synthesis of a series of triazolium salts, an evaluation of their antifungal activity, and docking studies. Ketoconazole and bifonazole were used as reference drugs. All compounds showed good antifungal activity with MIC/MFC in the range of 0.0003 to 0.2/0.0006-0.4 mg/mL. Compound 19 exhibited the best activity among all tested with MIC/MFC in the range of 0.009 to 0.037 mg/mL and 0.0125-0.05 mg/mL, respectively. All compounds appeared to be more potent than both reference drugs. The docking studies are in accordance with experimental results.",
publisher = "Basel: MDPI",
journal = "Antibiotics (Basel, Switzerland)",
title = "The Synthesis of Triazolium Salts as Antifungal Agents: A Biological and In Silico Evaluation.",
number = "5",
volume = "11",
doi = "10.3390/antibiotics11050588",
pages = "588"
}

Pogrebnoi, S., Radul, O., Stingaci, E., Lupascu, L., Valica, V., Uncu, L., Smetanscaia, A., Petrou, A., Ćirić, A., Glamočlija, J., Soković, M., Geronikaki, A.,& Macaev, F. Z.. (2022). The Synthesis of Triazolium Salts as Antifungal Agents: A Biological and In Silico Evaluation.. in Antibiotics (Basel, Switzerland)
Basel: MDPI., 11(5), 588.
https://doi.org/10.3390/antibiotics11050588

Pogrebnoi S, Radul O, Stingaci E, Lupascu L, Valica V, Uncu L, Smetanscaia A, Petrou A, Ćirić A, Glamočlija J, Soković M, Geronikaki A, Macaev FZ. The Synthesis of Triazolium Salts as Antifungal Agents: A Biological and In Silico Evaluation.. in Antibiotics (Basel, Switzerland). 2022;11(5):588.
doi:10.3390/antibiotics11050588 .

Pogrebnoi, Serghei, Radul, Oleg, Stingaci, Eugenia, Lupascu, Lucian, Valica, Vladimir, Uncu, Livia, Smetanscaia, Anastasia, Petrou, Anthi, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, Geronikaki, Athina, Macaev, Fliur Z., "The Synthesis of Triazolium Salts as Antifungal Agents: A Biological and In Silico Evaluation." in Antibiotics (Basel, Switzerland), 11, no. 5 (2022):588,
https://doi.org/10.3390/antibiotics11050588 . .

TY  - JOUR
AU  - Tsasi, Gerasimia
AU  - Danalatos, Gerasimos
AU  - Tomou, Ekaterina-Michaela
AU  - Sakadani, Eleni
AU  - Bethanis, Mihalis
AU  - Kolokouris, Aristides
AU  - Samaras, Yiannis
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Skaltsa, Helen
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/10412905.2022.2032421
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4791
AB  - Abies cephalonica Loudon consists one of the three fir species occurring in Greece. The aim of this study was to evaluate the chemical composition of the essential oil (EO) from needles of A. cephalonica from Mt. Aenos (Kefalonia Island, Greece) and to investigate its antimicrobial activity. The EO was obtained by hydrodistillation and 68 constituents corresponding to 99.4% of the EO were identified by using GC-MS analysis. The major chemical category of the volatile compounds were the monoterpene hydrocarbons (63.9%), followed by oxygenated monoterpenes (20.2%). In particular, the main components were beta-pinene (26.9%), bornyl acetate (12.5%), alpha-pinene (10.1%), camphene (9.2%), limonene (8.1%), gamma-eudesmol (8.1%) and beta-phellandrene (5.8%). To the best of our knowledge, the present study is the first research, reporting the phytochemical profile and the antimicrobial potential of the EO of A. cephalonica from this distinct geographic area.
T2  - Journal of Essential Oil Research
T1  - Chemical composition and antimicrobial activity of the essential oil of Abies cephalonica Loudon from Mount Ainos (Kefalonia, Greece)
IS  - 2
VL  - 34
DO  - 10.1080/10412905.2022.2032421
SP  - 143
EP  - 147
ER  - 

@article{
author = "Tsasi, Gerasimia and Danalatos, Gerasimos and Tomou, Ekaterina-Michaela and Sakadani, Eleni and Bethanis, Mihalis and Kolokouris, Aristides and Samaras, Yiannis and Ćirić, Ana and Soković, Marina and Skaltsa, Helen",
year = "2022",
abstract = "Abies cephalonica Loudon consists one of the three fir species occurring in Greece. The aim of this study was to evaluate the chemical composition of the essential oil (EO) from needles of A. cephalonica from Mt. Aenos (Kefalonia Island, Greece) and to investigate its antimicrobial activity. The EO was obtained by hydrodistillation and 68 constituents corresponding to 99.4% of the EO were identified by using GC-MS analysis. The major chemical category of the volatile compounds were the monoterpene hydrocarbons (63.9%), followed by oxygenated monoterpenes (20.2%). In particular, the main components were beta-pinene (26.9%), bornyl acetate (12.5%), alpha-pinene (10.1%), camphene (9.2%), limonene (8.1%), gamma-eudesmol (8.1%) and beta-phellandrene (5.8%). To the best of our knowledge, the present study is the first research, reporting the phytochemical profile and the antimicrobial potential of the EO of A. cephalonica from this distinct geographic area.",
journal = "Journal of Essential Oil Research",
title = "Chemical composition and antimicrobial activity of the essential oil of Abies cephalonica Loudon from Mount Ainos (Kefalonia, Greece)",
number = "2",
volume = "34",
doi = "10.1080/10412905.2022.2032421",
pages = "143-147"
}

Tsasi, G., Danalatos, G., Tomou, E., Sakadani, E., Bethanis, M., Kolokouris, A., Samaras, Y., Ćirić, A., Soković, M.,& Skaltsa, H.. (2022). Chemical composition and antimicrobial activity of the essential oil of Abies cephalonica Loudon from Mount Ainos (Kefalonia, Greece). in Journal of Essential Oil Research, 34(2), 143-147.
https://doi.org/10.1080/10412905.2022.2032421

Tsasi G, Danalatos G, Tomou E, Sakadani E, Bethanis M, Kolokouris A, Samaras Y, Ćirić A, Soković M, Skaltsa H. Chemical composition and antimicrobial activity of the essential oil of Abies cephalonica Loudon from Mount Ainos (Kefalonia, Greece). in Journal of Essential Oil Research. 2022;34(2):143-147.
doi:10.1080/10412905.2022.2032421 .

Tsasi, Gerasimia, Danalatos, Gerasimos, Tomou, Ekaterina-Michaela, Sakadani, Eleni, Bethanis, Mihalis, Kolokouris, Aristides, Samaras, Yiannis, Ćirić, Ana, Soković, Marina, Skaltsa, Helen, "Chemical composition and antimicrobial activity of the essential oil of Abies cephalonica Loudon from Mount Ainos (Kefalonia, Greece)" in Journal of Essential Oil Research, 34, no. 2 (2022):143-147,
https://doi.org/10.1080/10412905.2022.2032421 . .

TY  - JOUR
AU  - Othman, Souha
AU  - Añibarro-Ortega, Mikel
AU  - Dias, Maria Inês
AU  - Ćirić, Ana
AU  - Mandim, Filipa
AU  - Soković, Marina
AU  - Ferreira, Isabel C F R
AU  - Pinela, José
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9587281
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5175
AB  - Quince (Cydonia oblonga Mill.) is an astringent fruit widely processed into marmalade and other sweets through processes that discard the peel as a by-product. Therefore, this study was performed to characterize the quince peel composition in nutrients and phytochemicals and evaluate its in vitro biological activity, following a "zero waste" approach. The quince peel dry powder was particularly rich in fiber (20.2 g/100 g), fructose (34 g/100 g), malic acid (7.2 g/100 g), and potassium (692 mg/100 g). Extracts prepared by dynamic hydroethanolic maceration and hot water extraction yielded 4.70 and 4.27 mg/g of phenolic compounds, respectively, with a prevalence of flavan-3-ols. The hydroethanolic extract was the most effective in inhibiting lipid peroxidation and oxidative hemolysis, and also presented better antimicrobial effects against foodborne pathogens, which agreed with the highest flavan-3-ol contents. The extracts were better than control synthetic food additives against some tested fungal and bacterial strains. On the other hand, no ability to inhibit nitric oxide production or toxicity to the tumor and non-tumor cell lines was observed. Furthermore, the solid residues remaining after extraction contained 35-37 g/100 g of fiber. Overall, quince peel can be upcycled into fiber-rich and bioactive ingredients to endow the value chain with natural food fortifiers, preservatives, and health promoters.
PB  - Elsevier Ltd.
T2  - Heliyon
T1  - Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems
IS  - 10
VL  - 8
DO  - 10.1016/j.heliyon.2022.e11042
SP  - e11042
ER  - 

@article{
author = "Othman, Souha and Añibarro-Ortega, Mikel and Dias, Maria Inês and Ćirić, Ana and Mandim, Filipa and Soković, Marina and Ferreira, Isabel C F R and Pinela, José and Barros, Lillian",
year = "2022",
abstract = "Quince (Cydonia oblonga Mill.) is an astringent fruit widely processed into marmalade and other sweets through processes that discard the peel as a by-product. Therefore, this study was performed to characterize the quince peel composition in nutrients and phytochemicals and evaluate its in vitro biological activity, following a "zero waste" approach. The quince peel dry powder was particularly rich in fiber (20.2 g/100 g), fructose (34 g/100 g), malic acid (7.2 g/100 g), and potassium (692 mg/100 g). Extracts prepared by dynamic hydroethanolic maceration and hot water extraction yielded 4.70 and 4.27 mg/g of phenolic compounds, respectively, with a prevalence of flavan-3-ols. The hydroethanolic extract was the most effective in inhibiting lipid peroxidation and oxidative hemolysis, and also presented better antimicrobial effects against foodborne pathogens, which agreed with the highest flavan-3-ol contents. The extracts were better than control synthetic food additives against some tested fungal and bacterial strains. On the other hand, no ability to inhibit nitric oxide production or toxicity to the tumor and non-tumor cell lines was observed. Furthermore, the solid residues remaining after extraction contained 35-37 g/100 g of fiber. Overall, quince peel can be upcycled into fiber-rich and bioactive ingredients to endow the value chain with natural food fortifiers, preservatives, and health promoters.",
publisher = "Elsevier Ltd.",
journal = "Heliyon",
title = "Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems",
number = "10",
volume = "8",
doi = "10.1016/j.heliyon.2022.e11042",
pages = "e11042"
}

Othman, S., Añibarro-Ortega, M., Dias, M. I., Ćirić, A., Mandim, F., Soković, M., Ferreira, I. C. F. R., Pinela, J.,& Barros, L.. (2022). Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems. in Heliyon
Elsevier Ltd.., 8(10), e11042.
https://doi.org/10.1016/j.heliyon.2022.e11042

Othman S, Añibarro-Ortega M, Dias MI, Ćirić A, Mandim F, Soković M, Ferreira ICFR, Pinela J, Barros L. Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems. in Heliyon. 2022;8(10):e11042.
doi:10.1016/j.heliyon.2022.e11042 .

Othman, Souha, Añibarro-Ortega, Mikel, Dias, Maria Inês, Ćirić, Ana, Mandim, Filipa, Soković, Marina, Ferreira, Isabel C F R, Pinela, José, Barros, Lillian, "Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems" in Heliyon, 8, no. 10 (2022):e11042,
https://doi.org/10.1016/j.heliyon.2022.e11042 . .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Ćirić, Ana
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Anđelković, Boban
AU  - Todosijević, Marina
AU  - Todorović, Slađana
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Tešević, Vele
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Kanellis, Angelos K.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669022004757
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4983
AB  - Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites , such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus . Histochemical staining of leaves' trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus , grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) and two Gram-positive bacteria ( Bacillus cereus and Micrococcus flavus ). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent -3 β -acetoxy-13- epi -manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent -3 β -acetoxy-13- epi -manoyl oxide and its precursor ent -3 β -hydroxy-13- epi -manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”
VL  - 184
DO  - 10.1016/j.indcrop.2022.114992
SP  - 114992
ER  - 

@article{
author = "Skorić, Marijana and Ćirić, Ana and Budimir, Snežana and Janošević, Dušica and Anđelković, Boban and Todosijević, Marina and Todorović, Slađana and Soković, Marina and Glamočlija, Jasmina and Tešević, Vele and Gašić, Uroš and Mišić, Danijela and Kanellis, Angelos K.",
year = "2022",
abstract = "Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites , such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus . Histochemical staining of leaves' trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus , grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) and two Gram-positive bacteria ( Bacillus cereus and Micrococcus flavus ). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent -3 β -acetoxy-13- epi -manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent -3 β -acetoxy-13- epi -manoyl oxide and its precursor ent -3 β -hydroxy-13- epi -manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”",
volume = "184",
doi = "10.1016/j.indcrop.2022.114992",
pages = "114992"
}

Skorić, M., Ćirić, A., Budimir, S., Janošević, D., Anđelković, B., Todosijević, M., Todorović, S., Soković, M., Glamočlija, J., Tešević, V., Gašić, U., Mišić, D.,& Kanellis, A. K.. (2022). Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products
Elsevier B.V.., 184, 114992.
https://doi.org/10.1016/j.indcrop.2022.114992

Skorić M, Ćirić A, Budimir S, Janošević D, Anđelković B, Todosijević M, Todorović S, Soković M, Glamočlija J, Tešević V, Gašić U, Mišić D, Kanellis AK. Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products. 2022;184:114992.
doi:10.1016/j.indcrop.2022.114992 .

Skorić, Marijana, Ćirić, Ana, Budimir, Snežana, Janošević, Dušica, Anđelković, Boban, Todosijević, Marina, Todorović, Slađana, Soković, Marina, Glamočlija, Jasmina, Tešević, Vele, Gašić, Uroš, Mišić, Danijela, Kanellis, Angelos K., "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”" in Industrial Crops and Products, 184 (2022):114992,
https://doi.org/10.1016/j.indcrop.2022.114992 . .

TY  - JOUR
AU  - Milutinović, Violeta M.
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Ušjak, Ljuboš J.
AU  - Niketić, Marjan S.
AU  - Petrović, Silvana D.
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202200326
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5005
AB  - Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2 Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7-2.5 mg/mL MBC=3.4-5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15-0.4 mg/mL MFC=0.3-0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50 =148.1 μg/mL for SCH, and 152.3-303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50 =46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.
T2  - Chemistry & Biodiversity
T1  - Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.
IS  - 7
VL  - 19
DO  - 10.1002/cbdv.202200326
SP  - e202200326
ER  - 

@article{
author = "Milutinović, Violeta M. and Matić, Ivana Z. and Stanojković, Tatjana P. and Soković, Marina and Ćirić, Ana and Ušjak, Ljuboš J. and Niketić, Marjan S. and Petrović, Silvana D.",
year = "2022",
abstract = "Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2 Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7-2.5 mg/mL MBC=3.4-5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15-0.4 mg/mL MFC=0.3-0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50 =148.1 μg/mL for SCH, and 152.3-303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50 =46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.",
journal = "Chemistry & Biodiversity",
title = "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.",
number = "7",
volume = "19",
doi = "10.1002/cbdv.202200326",
pages = "e202200326"
}

Milutinović, V. M., Matić, I. Z., Stanojković, T. P., Soković, M., Ćirić, A., Ušjak, L. J., Niketić, M. S.,& Petrović, S. D.. (2022). Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.. in Chemistry & Biodiversity, 19(7), e202200326.
https://doi.org/10.1002/cbdv.202200326

Milutinović VM, Matić IZ, Stanojković TP, Soković M, Ćirić A, Ušjak LJ, Niketić MS, Petrović SD. Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.. in Chemistry & Biodiversity. 2022;19(7):e202200326.
doi:10.1002/cbdv.202200326 .

Milutinović, Violeta M., Matić, Ivana Z., Stanojković, Tatjana P., Soković, Marina, Ćirić, Ana, Ušjak, Ljuboš J., Niketić, Marjan S., Petrović, Silvana D., "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones." in Chemistry & Biodiversity, 19, no. 7 (2022):e202200326,
https://doi.org/10.1002/cbdv.202200326 . .

TY  - JOUR
AU  - Merlani, M.
AU  - Barbakadze, V.
AU  - Amiranashvili, L.
AU  - Gogilashvili, L.
AU  - Petrou, A.
AU  - Geronikaki, A.
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/1062936X.2022.2066173
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4969
AB  - Herein we report the evaluation of the antimicrobial activity of some previously synthesized 3-(3,4-dihydroxyphenyl)glyceric acid in benzylated and in free 3,4 hydroxy groups in catechol moiety along with some caffeic and 3-(3,4-dihydroxyphenyl)glyceric acid amides using the microdilution method. The evaluation revealed that compounds showed in general moderate to low activity with MIC in range of 0.36-4.5 mg/mL. Compounds were also studied against three resistant bacteria strains MRSA (Methicillin-resistant Staphylococcus aureus), E. coli and P. aeruginosa. Seven out of ten compounds were more potent than reference drugs ampicillin and streptomycin against MRSA, while against another two resistant strains seven compounds showed low activity and the rest were inactive. Antifungal activity of the tested compounds was much better than antibacterial, with MIC in the range of 0.019-3.0 mg/mL. Compounds #7 and 15 showed good activity against all fungi tested, being more potent than ketoconazole and in some case even better than bifonazole used as reference drugs. Docking studies revealed that the most active compound #7 binds to the haem group of the enzyme in the same way as ketoconazole.
T2  - SAR and QSAR in Environmental Research
T1  - Caffeic and 3-(3,4-dihydroxyphenyl)glyceric acid derivatives as antimicrobial agent: biological evaluation and molecular docking studies.
IS  - 4
VL  - 33
DO  - 10.1080/1062936X.2022.2066173
SP  - 307
EP  - 321
ER  - 

@article{
author = "Merlani, M. and Barbakadze, V. and Amiranashvili, L. and Gogilashvili, L. and Petrou, A. and Geronikaki, A. and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Herein we report the evaluation of the antimicrobial activity of some previously synthesized 3-(3,4-dihydroxyphenyl)glyceric acid in benzylated and in free 3,4 hydroxy groups in catechol moiety along with some caffeic and 3-(3,4-dihydroxyphenyl)glyceric acid amides using the microdilution method. The evaluation revealed that compounds showed in general moderate to low activity with MIC in range of 0.36-4.5 mg/mL. Compounds were also studied against three resistant bacteria strains MRSA (Methicillin-resistant Staphylococcus aureus), E. coli and P. aeruginosa. Seven out of ten compounds were more potent than reference drugs ampicillin and streptomycin against MRSA, while against another two resistant strains seven compounds showed low activity and the rest were inactive. Antifungal activity of the tested compounds was much better than antibacterial, with MIC in the range of 0.019-3.0 mg/mL. Compounds #7 and 15 showed good activity against all fungi tested, being more potent than ketoconazole and in some case even better than bifonazole used as reference drugs. Docking studies revealed that the most active compound #7 binds to the haem group of the enzyme in the same way as ketoconazole.",
journal = "SAR and QSAR in Environmental Research",
title = "Caffeic and 3-(3,4-dihydroxyphenyl)glyceric acid derivatives as antimicrobial agent: biological evaluation and molecular docking studies.",
number = "4",
volume = "33",
doi = "10.1080/1062936X.2022.2066173",
pages = "307-321"
}

Merlani, M., Barbakadze, V., Amiranashvili, L., Gogilashvili, L., Petrou, A., Geronikaki, A., Ćirić, A., Glamočlija, J.,& Soković, M.. (2022). Caffeic and 3-(3,4-dihydroxyphenyl)glyceric acid derivatives as antimicrobial agent: biological evaluation and molecular docking studies.. in SAR and QSAR in Environmental Research, 33(4), 307-321.
https://doi.org/10.1080/1062936X.2022.2066173

Merlani M, Barbakadze V, Amiranashvili L, Gogilashvili L, Petrou A, Geronikaki A, Ćirić A, Glamočlija J, Soković M. Caffeic and 3-(3,4-dihydroxyphenyl)glyceric acid derivatives as antimicrobial agent: biological evaluation and molecular docking studies.. in SAR and QSAR in Environmental Research. 2022;33(4):307-321.
doi:10.1080/1062936X.2022.2066173 .

Merlani, M., Barbakadze, V., Amiranashvili, L., Gogilashvili, L., Petrou, A., Geronikaki, A., Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "Caffeic and 3-(3,4-dihydroxyphenyl)glyceric acid derivatives as antimicrobial agent: biological evaluation and molecular docking studies." in SAR and QSAR in Environmental Research, 33, no. 4 (2022):307-321,
https://doi.org/10.1080/1062936X.2022.2066173 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
AU  - Mitić-Ćulafić, Dragana
AU  - Dukanović, Stefana
AU  - Krstić, Aleksandar
AU  - Pavlica, Dušan
AU  - Vlajić, Tamara
AU  - Marković, Dejan
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/0972060X.2022.2040386
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4944
AB  - The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.
PB  - Abingdon: Taylor & Francis Ltd.
T2  - Journal of Essential Oil-Bearing Plants
T1  - Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes
IS  - 1
VL  - 25
DO  - 10.1080/0972060X.2022.2040386
SP  - 111
EP  - 125
ER  - 

@article{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Ćirić, Ana and Mitić-Ćulafić, Dragana and Dukanović, Stefana and Krstić, Aleksandar and Pavlica, Dušan and Vlajić, Tamara and Marković, Dejan",
year = "2022",
abstract = "The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.",
publisher = "Abingdon: Taylor & Francis Ltd.",
journal = "Journal of Essential Oil-Bearing Plants",
title = "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes",
number = "1",
volume = "25",
doi = "10.1080/0972060X.2022.2040386",
pages = "111-125"
}

Marinković, J., Marković, T., Nikolić, B., Ćirić, A., Mitić-Ćulafić, D., Dukanović, S., Krstić, A., Pavlica, D., Vlajić, T.,& Marković, D.. (2022). Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants
Abingdon: Taylor & Francis Ltd.., 25(1), 111-125.
https://doi.org/10.1080/0972060X.2022.2040386

Marinković J, Marković T, Nikolić B, Ćirić A, Mitić-Ćulafić D, Dukanović S, Krstić A, Pavlica D, Vlajić T, Marković D. Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants. 2022;25(1):111-125.
doi:10.1080/0972060X.2022.2040386 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Ćirić, Ana, Mitić-Ćulafić, Dragana, Dukanović, Stefana, Krstić, Aleksandar, Pavlica, Dušan, Vlajić, Tamara, Marković, Dejan, "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes" in Journal of Essential Oil-Bearing Plants, 25, no. 1 (2022):111-125,
https://doi.org/10.1080/0972060X.2022.2040386 . .