TY  - JOUR
AU  - Aničić, Neda
AU  - Mišić, Danijela
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6552
AB  - The genus Nepeta, belonging to the Lamiaceae family, encompasses a diverse group of plants with significant
biological activities attributed mainly to their iridoid compounds. This review provides a comprehensive analysis
of recent research on iridoid biosynthesis, regulation, and evolutionary aspects within the Nepeta genus. The biological
activities of Nepeta species, including repellent, phytotoxic, antimicrobial, and cytotoxic effects, have been extensively
investigated, highlighting the potential applications of iridoids. Over the past decade, significant progress has been
made in elucidating the molecular basis of iridoid biosynthesis and regulation, thanks to advancements in transcriptomics,
genomics, and metabolomics. The presence of distinct chemotype groups within Nepeta has been revealed,
characterized by their ability to produce both iridoid aglycones (nepetalactones) and glycosylated iridoids (IAs and
IGs), exclusively produce IGs, or lack iridoids. The identification of key enzymes involved in iridoid biosynthesis, such
as geraniol synthase (GES) and iridoid synthase (ISY), has played a crucial role in understanding the pathway. Furthermore,
the evolutionary aspects of the iridoid biosynthesis loss in some of the Nepeta taxa, and the association of iridoid
presence and content with the expression levels of specific genes, have been investigated. However, several areas remain
to be explored, including the final steps of iridoid aglycones biosynthetic branch, the production of iridoid glucosides,
the role of transcription factors in fine-tuning of iridoid biosynthesis, and the intricate interplay between biosynthetic
enzymes. Continued research in these areas will deepen our understanding of iridoid metabolism in Nepeta and unlock
their full potential in various fields, including pharmaceuticals, agriculture, and natural product-based industries.
PB  - Novi Sad, Serbia: Biologia Serbica
T2  - Biologia Serbica
T1  - Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review
VL  - 46
DO  - 10.5281/zenodo.10606764
ER  - 

@article{
author = "Aničić, Neda and Mišić, Danijela",
year = "2024",
abstract = "The genus Nepeta, belonging to the Lamiaceae family, encompasses a diverse group of plants with significant
biological activities attributed mainly to their iridoid compounds. This review provides a comprehensive analysis
of recent research on iridoid biosynthesis, regulation, and evolutionary aspects within the Nepeta genus. The biological
activities of Nepeta species, including repellent, phytotoxic, antimicrobial, and cytotoxic effects, have been extensively
investigated, highlighting the potential applications of iridoids. Over the past decade, significant progress has been
made in elucidating the molecular basis of iridoid biosynthesis and regulation, thanks to advancements in transcriptomics,
genomics, and metabolomics. The presence of distinct chemotype groups within Nepeta has been revealed,
characterized by their ability to produce both iridoid aglycones (nepetalactones) and glycosylated iridoids (IAs and
IGs), exclusively produce IGs, or lack iridoids. The identification of key enzymes involved in iridoid biosynthesis, such
as geraniol synthase (GES) and iridoid synthase (ISY), has played a crucial role in understanding the pathway. Furthermore,
the evolutionary aspects of the iridoid biosynthesis loss in some of the Nepeta taxa, and the association of iridoid
presence and content with the expression levels of specific genes, have been investigated. However, several areas remain
to be explored, including the final steps of iridoid aglycones biosynthetic branch, the production of iridoid glucosides,
the role of transcription factors in fine-tuning of iridoid biosynthesis, and the intricate interplay between biosynthetic
enzymes. Continued research in these areas will deepen our understanding of iridoid metabolism in Nepeta and unlock
their full potential in various fields, including pharmaceuticals, agriculture, and natural product-based industries.",
publisher = "Novi Sad, Serbia: Biologia Serbica",
journal = "Biologia Serbica",
title = "Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review",
volume = "46",
doi = "10.5281/zenodo.10606764"
}

Aničić, N.,& Mišić, D.. (2024). Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review. in Biologia Serbica
Novi Sad, Serbia: Biologia Serbica., 46.
https://doi.org/10.5281/zenodo.10606764

Aničić N, Mišić D. Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review. in Biologia Serbica. 2024;46.
doi:10.5281/zenodo.10606764 .

Aničić, Neda, Mišić, Danijela, "Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review" in Biologia Serbica, 46 (2024),
https://doi.org/10.5281/zenodo.10606764 . .

TY  - CONF
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Lukić, Tamara
AU  - Gašić, Uroš
AU  - Petrović, Luka
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Božunović, Jelena
AU  - Filipović, Biljana
AU  - Todorović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - https://europlantbiology2023.org/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6481
AB  - Nepeta L. is the largest genus of the Lamiaceae family. The species of this genus are famous
for their biopesticidal potential and medicinal properties. Within the genus, we found
considerable variability in the qualitative and quantitative content of iridoid and phenolic
compounds. Using state-of-the-art metabolomics techniques (UHPLC/LTQ-Orbitrap MS,
GC/MS, UHPLC/DAD/(+/-)HESI-MS2), we comprehensively assessed the overall diversity of iridoids and phenolics in the selected chemodiverse Nepeta taxa at the intra-species level.
To reconstruct phylogenetic relations among the selected chemodiverse taxa from across the
Nepeta genus, genomic ITS molecular marker and two maternally inherited plastid DNA
regions (trnL-trnF and rbcL) were analyzed. A consensus phylogenetic tree was constructed
to interpret the distribution of the most abundant iridoid and phenolic compounds. It was
not possible to uncover clear regularities between phylogenetic relationship and chemotype
affiliation.
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta
SP  - 78
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6481
ER  - 

@conference{
author = "Banjanac, Tijana and Šiler, Branislav and Lukić, Tamara and Gašić, Uroš and Petrović, Luka and Matekalo, Dragana and Skorić, Marijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Milutinović, Milica and Aničić, Neda and Božunović, Jelena and Filipović, Biljana and Todorović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "Nepeta L. is the largest genus of the Lamiaceae family. The species of this genus are famous
for their biopesticidal potential and medicinal properties. Within the genus, we found
considerable variability in the qualitative and quantitative content of iridoid and phenolic
compounds. Using state-of-the-art metabolomics techniques (UHPLC/LTQ-Orbitrap MS,
GC/MS, UHPLC/DAD/(+/-)HESI-MS2), we comprehensively assessed the overall diversity of iridoids and phenolics in the selected chemodiverse Nepeta taxa at the intra-species level.
To reconstruct phylogenetic relations among the selected chemodiverse taxa from across the
Nepeta genus, genomic ITS molecular marker and two maternally inherited plastid DNA
regions (trnL-trnF and rbcL) were analyzed. A consensus phylogenetic tree was constructed
to interpret the distribution of the most abundant iridoid and phenolic compounds. It was
not possible to uncover clear regularities between phylogenetic relationship and chemotype
affiliation.",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta",
pages = "78-79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6481"
}

Banjanac, T., Šiler, B., Lukić, T., Gašić, U., Petrović, L., Matekalo, D., Skorić, M., Nestorović Živković, J., Dmitrović, S., Milutinović, M., Aničić, N., Božunović, J., Filipović, B., Todorović, M.,& Mišić, D.. (2023). Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_6481

Banjanac T, Šiler B, Lukić T, Gašić U, Petrović L, Matekalo D, Skorić M, Nestorović Živković J, Dmitrović S, Milutinović M, Aničić N, Božunović J, Filipović B, Todorović M, Mišić D. Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_6481 .

Banjanac, Tijana, Šiler, Branislav, Lukić, Tamara, Gašić, Uroš, Petrović, Luka, Matekalo, Dragana, Skorić, Marijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Milutinović, Milica, Aničić, Neda, Božunović, Jelena, Filipović, Biljana, Todorović, Miloš, Mišić, Danijela, "Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):78-79,
https://hdl.handle.net/21.15107/rcub_ibiss_6481 .

TY  - GEN
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Šiler, Branislav
AU  - Dmitrović, Slavica
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Petrović, Luka
AU  - Lukić, Tamara
AU  - Todorović, Miloš
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6560
AB  - Introduction: Recent intensive research on the genus Nepeta has resulted in increasingly accumulated
information regarding the biosynthetic pathways and chemical evolution of iridoids; however, several in-
triguing aspects remain yet to be resolved. Our objective was to deeply investigate the molecular back-
ground of the diversity of iridoid compounds within the genus Nepeta, as well as regulative mechanisms
of their biosynthesis.
Methods: Leaves of greenhouse-grown plants were analysed for the composition of iridoid aglycones
(IAs) and iridoid glucosides (IGs) adopting non-targeted (UHPLC/LTQ Orbitrap MS n
, GC/MS) and targeted
(UHPLC/DAD/(±)HESI-MS 2 ) metabolomics approaches. Following RNA-Seq, transcriptomes of phylodi-
verse Nepeta taxa were searched for the presence/absence of iridoid-related biosynthetic genes. Co-ex-
pression patterns (qPCR) of biosynthetic genes and transcription factors were determined following
plants’ exposure to various environmental stresses.
Results: The comparison of metabolite composition among phylodiverse Nepeta taxa provided a sys-
tematic understanding of qualitative and quantitative composition of iridoids in leaves of the selected
Nepeta taxa. Mining of RNA-Seq data in search for iridoid biosynthesis-related genes pointed to signifi-
cant differences between taxa producing both IAs and IGs and those producing only IGs. Comparative
metabolomics and gene co-expression analysis provided new information about mechanisms of regu-
lation of iridoid biosynthesis.
Conclusion: Integration of data from several techniques analysed by different methods resulted in iden-
tifying key genes involved in the regulation of metabolic flux through the iridoid biosynthetic pathway
and offered an explanation why some Nepeta taxa produce only IGs, while others produce both IAs and
IGs. The obtained results pointed to new gene targets for improving iridoid production through biotech-
nology-based approaches.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae)
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6560
ER  - 

@misc{
author = "Mišić, Danijela and Gašić, Uroš and Šiler, Branislav and Dmitrović, Slavica and Skorić, Marijana and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Aničić, Neda and Petrović, Luka and Lukić, Tamara and Todorović, Miloš",
year = "2023",
abstract = "Introduction: Recent intensive research on the genus Nepeta has resulted in increasingly accumulated
information regarding the biosynthetic pathways and chemical evolution of iridoids; however, several in-
triguing aspects remain yet to be resolved. Our objective was to deeply investigate the molecular back-
ground of the diversity of iridoid compounds within the genus Nepeta, as well as regulative mechanisms
of their biosynthesis.
Methods: Leaves of greenhouse-grown plants were analysed for the composition of iridoid aglycones
(IAs) and iridoid glucosides (IGs) adopting non-targeted (UHPLC/LTQ Orbitrap MS n
, GC/MS) and targeted
(UHPLC/DAD/(±)HESI-MS 2 ) metabolomics approaches. Following RNA-Seq, transcriptomes of phylodi-
verse Nepeta taxa were searched for the presence/absence of iridoid-related biosynthetic genes. Co-ex-
pression patterns (qPCR) of biosynthetic genes and transcription factors were determined following
plants’ exposure to various environmental stresses.
Results: The comparison of metabolite composition among phylodiverse Nepeta taxa provided a sys-
tematic understanding of qualitative and quantitative composition of iridoids in leaves of the selected
Nepeta taxa. Mining of RNA-Seq data in search for iridoid biosynthesis-related genes pointed to signifi-
cant differences between taxa producing both IAs and IGs and those producing only IGs. Comparative
metabolomics and gene co-expression analysis provided new information about mechanisms of regu-
lation of iridoid biosynthesis.
Conclusion: Integration of data from several techniques analysed by different methods resulted in iden-
tifying key genes involved in the regulation of metabolic flux through the iridoid biosynthetic pathway
and offered an explanation why some Nepeta taxa produce only IGs, while others produce both IAs and
IGs. The obtained results pointed to new gene targets for improving iridoid production through biotech-
nology-based approaches.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae)",
pages = "133",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6560"
}

Mišić, D., Gašić, U., Šiler, B., Dmitrović, S., Skorić, M., Matekalo, D., Nestorović Živković, J., Banjanac, T., Filipović, B., Milutinović, M., Božunović, J., Aničić, N., Petrović, L., Lukić, T.,& Todorović, M.. (2023). Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae). in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade., 133.
https://hdl.handle.net/21.15107/rcub_ibiss_6560

Mišić D, Gašić U, Šiler B, Dmitrović S, Skorić M, Matekalo D, Nestorović Živković J, Banjanac T, Filipović B, Milutinović M, Božunović J, Aničić N, Petrović L, Lukić T, Todorović M. Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae). in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;:133.
https://hdl.handle.net/21.15107/rcub_ibiss_6560 .

Mišić, Danijela, Gašić, Uroš, Šiler, Branislav, Dmitrović, Slavica, Skorić, Marijana, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Aničić, Neda, Petrović, Luka, Lukić, Tamara, Todorović, Miloš, "Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae)" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia (2023):133,
https://hdl.handle.net/21.15107/rcub_ibiss_6560 .

TY  - CONF
AU  - Mišić, Danijela
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Gašić, Uroš
AU  - Skorić, Marijana
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6083
AB  - Data on the distribution and diversity of iridoid glycosides (IGs) and iridoid aglycones (IAs) across the genus Nepeta (fam. Lamiaceae) are fragmentary; only rarely these two subgroups of iridoids are simultaneously analysed. Our ambition was to fulfil these knowledge gaps by adopting state-of-the-art metabolomics techniques (untargeted LC-HRMS and GC/MS) to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phyllo-diverse Nepeta taxa, and thus facilitate further reconstruction of the molecular background of this diversity. Analyzed taxa were robustly grouped into three major chemotypes: (A) producers of both IGs and IAs; (B) taxa exclusively producing IGs; and (C) iridoid-lacking taxa. Furthermore, Nepeta species belonging to the chemotype A displayed significant heterogeneity in their IAs composition, and were roughly classified into several sub-chemotypes based on the stereochemistry of the major nepetalactone. No clear regularities between chemotype affiliation and phylogenetic relatedness, reconstructed adopting DNA barcoding tools, was revealed. Transcriptomes of leaves of distinguished Nepeta chemotypes were mined for the transcripts of iridoid-related biosynthetic genes, to facilitate the assessment of the factors responsible for the presence/absence of iridoids, or specific subgroups of iridoids, in these plants. Integration of metabolomics and transcriptomics data pointed to several key genes in the biosynthetic pathway as significant determinants of the iridoid diversity within the genus: geraniol synthase (GES), iridoid synthase (ISY), NAD-dependent nepetalactol-related short-chain-dehydrogenases/reductases (NEPSs), and latex protein-like genes (MLPLs). This study provided a framework to revisit some of the iridoid-metabolism-related ecological and chemical evolution theories, which is the course of our further work.
PB  - Davis: University of California
C3  - Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
T1  - Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae)
SP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6083
ER  - 

@conference{
author = "Mišić, Danijela and Aničić, Neda and Matekalo, Dragana and Gašić, Uroš and Skorić, Marijana and Šiler, Branislav and Banjanac, Tijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Petrović, Luka and Todorović, Miloš and Lukić, Tamara",
year = "2023",
abstract = "Data on the distribution and diversity of iridoid glycosides (IGs) and iridoid aglycones (IAs) across the genus Nepeta (fam. Lamiaceae) are fragmentary; only rarely these two subgroups of iridoids are simultaneously analysed. Our ambition was to fulfil these knowledge gaps by adopting state-of-the-art metabolomics techniques (untargeted LC-HRMS and GC/MS) to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phyllo-diverse Nepeta taxa, and thus facilitate further reconstruction of the molecular background of this diversity. Analyzed taxa were robustly grouped into three major chemotypes: (A) producers of both IGs and IAs; (B) taxa exclusively producing IGs; and (C) iridoid-lacking taxa. Furthermore, Nepeta species belonging to the chemotype A displayed significant heterogeneity in their IAs composition, and were roughly classified into several sub-chemotypes based on the stereochemistry of the major nepetalactone. No clear regularities between chemotype affiliation and phylogenetic relatedness, reconstructed adopting DNA barcoding tools, was revealed. Transcriptomes of leaves of distinguished Nepeta chemotypes were mined for the transcripts of iridoid-related biosynthetic genes, to facilitate the assessment of the factors responsible for the presence/absence of iridoids, or specific subgroups of iridoids, in these plants. Integration of metabolomics and transcriptomics data pointed to several key genes in the biosynthetic pathway as significant determinants of the iridoid diversity within the genus: geraniol synthase (GES), iridoid synthase (ISY), NAD-dependent nepetalactol-related short-chain-dehydrogenases/reductases (NEPSs), and latex protein-like genes (MLPLs). This study provided a framework to revisit some of the iridoid-metabolism-related ecological and chemical evolution theories, which is the course of our further work.",
publisher = "Davis: University of California",
journal = "Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA",
title = "Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae)",
pages = "100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6083"
}

Mišić, D., Aničić, N., Matekalo, D., Gašić, U., Skorić, M., Šiler, B., Banjanac, T., Nestorović Živković, J., Dmitrović, S., Filipović, B., Milutinović, M., Božunović, J., Petrović, L., Todorović, M.,& Lukić, T.. (2023). Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae). in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
Davis: University of California., 100.
https://hdl.handle.net/21.15107/rcub_ibiss_6083

Mišić D, Aničić N, Matekalo D, Gašić U, Skorić M, Šiler B, Banjanac T, Nestorović Živković J, Dmitrović S, Filipović B, Milutinović M, Božunović J, Petrović L, Todorović M, Lukić T. Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae). in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. 2023;:100.
https://hdl.handle.net/21.15107/rcub_ibiss_6083 .

Mišić, Danijela, Aničić, Neda, Matekalo, Dragana, Gašić, Uroš, Skorić, Marijana, Šiler, Branislav, Banjanac, Tijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, "Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae)" in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA (2023):100,
https://hdl.handle.net/21.15107/rcub_ibiss_6083 .

TY  - CONF
AU  - Mišić, Danijela
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Gašić, Uroš
AU  - Skorić, Marijana
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6480
AB  - Aim: The chemical richness of iridoids in the genus Nepeta, the exclusive representative of the subfamily Nepetoidae (Lamiaceae) that produces iridoids, has complex roles in nature, facilitating interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavour additives, fragrances, as well as toys for cats, and their bioactivities are usually assigned to nepetalactones. Data on the distribution and diversity of iridoids across the genus Nepeta are quite fragmental and only rarely, the two subgroups, iridoid glycosides (IGs) and iridoid aglycones (IAs), are simultaneously analysed. Our aim was to develop and adopt non-targeted metabolomics methods to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phylodiverse Nepeta taxa, and thus facilitate reconstruction of this diversity at the molecular level.
Material and methods: Metabolomics data were acquired adopting UHPLC/Orbitrap MS, UHPLC/QToF MS, and GC/MS. Leaves of phylodiverse/chemodiverse Nepeta taxa were subjected to RNA-Seq. DNA barcoding analysis was performed using chloroplast markers (matK, rbcL, trnL-trnF) and nuclear ribosomal ITS marker.
Main results: Nepeta taxa were robustly grouped into three major chemotypes: (1) producers of both IGs and IAs; (2) taxa exclusively producing IGs; and (3) taxa lacking iridoids. Chemotype A clade was further roughly classified into several sub-chemotypes based on the stereochemistry of the major compound  nepetalactone. Phylogenetic relations between the analysed taxa were reconstructed adopting a selected set of DNA barcoding markers. Transcriptomes of leaves of the distinguished Nepeta chemotypes were searched for transcripts of iridoid-related biosynthetic genes to facilitate the assessment of factors responsible for the presence/absence of IAs and IGs in these plants.
Conclusion: Integration of metabolomics, transcriptomics and DNA barcoding data revealed no clear regularities between chemotype affiliation and phylogenetic relatedness in analysed Nepeta taxa and pointed to several key genes in the iridoid’s biosynthetic pathway as significant determinants of the their diversity within the genus.
PB  - Farrago
C3  - Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria
T1  - Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae)
SP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6480
ER  - 

@conference{
author = "Mišić, Danijela and Aničić, Neda and Matekalo, Dragana and Gašić, Uroš and Skorić, Marijana and Šiler, Branislav and Banjanac, Tijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Petrović, Luka and Todorović, Miloš and Lukić, Tamara",
year = "2023",
abstract = "Aim: The chemical richness of iridoids in the genus Nepeta, the exclusive representative of the subfamily Nepetoidae (Lamiaceae) that produces iridoids, has complex roles in nature, facilitating interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavour additives, fragrances, as well as toys for cats, and their bioactivities are usually assigned to nepetalactones. Data on the distribution and diversity of iridoids across the genus Nepeta are quite fragmental and only rarely, the two subgroups, iridoid glycosides (IGs) and iridoid aglycones (IAs), are simultaneously analysed. Our aim was to develop and adopt non-targeted metabolomics methods to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phylodiverse Nepeta taxa, and thus facilitate reconstruction of this diversity at the molecular level.
Material and methods: Metabolomics data were acquired adopting UHPLC/Orbitrap MS, UHPLC/QToF MS, and GC/MS. Leaves of phylodiverse/chemodiverse Nepeta taxa were subjected to RNA-Seq. DNA barcoding analysis was performed using chloroplast markers (matK, rbcL, trnL-trnF) and nuclear ribosomal ITS marker.
Main results: Nepeta taxa were robustly grouped into three major chemotypes: (1) producers of both IGs and IAs; (2) taxa exclusively producing IGs; and (3) taxa lacking iridoids. Chemotype A clade was further roughly classified into several sub-chemotypes based on the stereochemistry of the major compound  nepetalactone. Phylogenetic relations between the analysed taxa were reconstructed adopting a selected set of DNA barcoding markers. Transcriptomes of leaves of the distinguished Nepeta chemotypes were searched for transcripts of iridoid-related biosynthetic genes to facilitate the assessment of factors responsible for the presence/absence of IAs and IGs in these plants.
Conclusion: Integration of metabolomics, transcriptomics and DNA barcoding data revealed no clear regularities between chemotype affiliation and phylogenetic relatedness in analysed Nepeta taxa and pointed to several key genes in the iridoid’s biosynthetic pathway as significant determinants of the their diversity within the genus.",
publisher = "Farrago",
journal = "Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria",
title = "Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae)",
pages = "77",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6480"
}

Mišić, D., Aničić, N., Matekalo, D., Gašić, U., Skorić, M., Šiler, B., Banjanac, T., Nestorović Živković, J., Dmitrović, S., Filipović, B., Milutinović, M., Božunović, J., Petrović, L., Todorović, M.,& Lukić, T.. (2023). Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae). in Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria
Farrago., 77.
https://hdl.handle.net/21.15107/rcub_ibiss_6480

Mišić D, Aničić N, Matekalo D, Gašić U, Skorić M, Šiler B, Banjanac T, Nestorović Živković J, Dmitrović S, Filipović B, Milutinović M, Božunović J, Petrović L, Todorović M, Lukić T. Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae). in Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria. 2023;:77.
https://hdl.handle.net/21.15107/rcub_ibiss_6480 .

Mišić, Danijela, Aničić, Neda, Matekalo, Dragana, Gašić, Uroš, Skorić, Marijana, Šiler, Branislav, Banjanac, Tijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, "Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae)" in Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria (2023):77,
https://hdl.handle.net/21.15107/rcub_ibiss_6480 .

TY  - CONF
AU  - Šiler, Branislav
AU  - Petrović, Luka
AU  - Banjanac, Tijana
AU  - Lukić, Tamara
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Božunović, Jelena
AU  - Filipović, Biljana
AU  - Todorović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - https://europlantbiology2023.org/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6443
AB  - Numerous species belonging to the genus Nepeta are appreciated producers of various
phenolic and iridoid compounds that have tremendously vast applicative value in
pharmacology and agriculture but are also highly esteemed as chemotaxonomical markers.
Nepeta nuda is a widespread Eurasian species, found in forest edges and clearings. We have
collected leaf samples from 12 populations across the central Balkan Peninsula and
investigated their genetic and phytochemical variations. We used 3 genomic and 7 transcriptome-derived microsatellite markers and revealed relatively low variation within the
populations. Both qualitative metabolite profiling of methanol extracts (Q-TOFF LC/MS) and
essential oils (GC/MS) analysis pointed out to high inter- and intra-population variations,
especially regarding the content of iridoids. A UHPLC-QQQ-MS/MS approach targeted towards
quantification of the most abundant iridoids and phenolics was highly efficient in the
diversification of N. nuda populations.
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans
SP  - 186
EP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6443
ER  - 

@conference{
author = "Šiler, Branislav and Petrović, Luka and Banjanac, Tijana and Lukić, Tamara and Matekalo, Dragana and Skorić, Marijana and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Milutinović, Milica and Aničić, Neda and Božunović, Jelena and Filipović, Biljana and Todorović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "Numerous species belonging to the genus Nepeta are appreciated producers of various
phenolic and iridoid compounds that have tremendously vast applicative value in
pharmacology and agriculture but are also highly esteemed as chemotaxonomical markers.
Nepeta nuda is a widespread Eurasian species, found in forest edges and clearings. We have
collected leaf samples from 12 populations across the central Balkan Peninsula and
investigated their genetic and phytochemical variations. We used 3 genomic and 7 transcriptome-derived microsatellite markers and revealed relatively low variation within the
populations. Both qualitative metabolite profiling of methanol extracts (Q-TOFF LC/MS) and
essential oils (GC/MS) analysis pointed out to high inter- and intra-population variations,
especially regarding the content of iridoids. A UHPLC-QQQ-MS/MS approach targeted towards
quantification of the most abundant iridoids and phenolics was highly efficient in the
diversification of N. nuda populations.",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans",
pages = "186-187",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6443"
}

Šiler, B., Petrović, L., Banjanac, T., Lukić, T., Matekalo, D., Skorić, M., Gašić, U., Nestorović Živković, J., Dmitrović, S., Milutinović, M., Aničić, N., Božunović, J., Filipović, B., Todorović, M.,& Mišić, D.. (2023). Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 186-187.
https://hdl.handle.net/21.15107/rcub_ibiss_6443

Šiler B, Petrović L, Banjanac T, Lukić T, Matekalo D, Skorić M, Gašić U, Nestorović Živković J, Dmitrović S, Milutinović M, Aničić N, Božunović J, Filipović B, Todorović M, Mišić D. Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:186-187.
https://hdl.handle.net/21.15107/rcub_ibiss_6443 .

Šiler, Branislav, Petrović, Luka, Banjanac, Tijana, Lukić, Tamara, Matekalo, Dragana, Skorić, Marijana, Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Milutinović, Milica, Aničić, Neda, Božunović, Jelena, Filipović, Biljana, Todorović, Miloš, Mišić, Danijela, "Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):186-187,
https://hdl.handle.net/21.15107/rcub_ibiss_6443 .

TY  - GEN
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Dmitrović, Slavica
AU  - Petrović, Luka
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Anđelković, Boban
AU  - Dimitrijević, Milena
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6196
AB  - Nepeta L. holds a prominent position as the largest genus in the Lamiaceae family and serves as the exclusive representative of the iridoid-lacking Nepetoidae subfamily which produces iridoids. The genus showcases a rich chemodiversity, encompassing taxa both with and without iridoids. Our study investigates the genetic basis of iridoid diversity using omics-guided and functional genomics approaches. We identified functional iridoid synthases (ISY) in iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa L. (NnIS). Remarkably, N. nervosa carries a dormant iridoid biosynthetic platform, suggesting a loss of iridoid production during its evolutionary history. Moreover, we explore regulatory mechanisms through comparative iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs) under various stress conditions (e.g., dehydration, UV − B radiation, pathogens) or elicitors (MeJA). These mechanisms influence plant productivity and the presence/absence of iridoids or their specific groups (iridoid aglycones and iridoid glycosides). Our research offers valuable insights into the molecular mechanisms driving iridoid biosynthesis and the chemical evolution of iridoids within Nepeta. This study enhances our understanding of the intricate relationship between genetics and the environment, providing comprehensive insights into iridoid metabolism, chemical evolution, and ecology.
PB  - Belgrade: Faculty of Chemistry
T2  - Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
T1  - Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6196
ER  - 

@misc{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Božunović, Jelena and Nestorović Živković, Jasmina and Gašić, Uroš and Milutinović, Milica and Dmitrović, Slavica and Petrović, Luka and Filipović, Biljana and Banjanac, Tijana and Šiler, Branislav and Anđelković, Boban and Dimitrijević, Milena and Mišić, Danijela",
year = "2023",
abstract = "Nepeta L. holds a prominent position as the largest genus in the Lamiaceae family and serves as the exclusive representative of the iridoid-lacking Nepetoidae subfamily which produces iridoids. The genus showcases a rich chemodiversity, encompassing taxa both with and without iridoids. Our study investigates the genetic basis of iridoid diversity using omics-guided and functional genomics approaches. We identified functional iridoid synthases (ISY) in iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa L. (NnIS). Remarkably, N. nervosa carries a dormant iridoid biosynthetic platform, suggesting a loss of iridoid production during its evolutionary history. Moreover, we explore regulatory mechanisms through comparative iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs) under various stress conditions (e.g., dehydration, UV − B radiation, pathogens) or elicitors (MeJA). These mechanisms influence plant productivity and the presence/absence of iridoids or their specific groups (iridoid aglycones and iridoid glycosides). Our research offers valuable insights into the molecular mechanisms driving iridoid biosynthesis and the chemical evolution of iridoids within Nepeta. This study enhances our understanding of the intricate relationship between genetics and the environment, providing comprehensive insights into iridoid metabolism, chemical evolution, and ecology.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
title = "Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme",
pages = "156",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6196"
}

Aničić, N., Matekalo, D., Skorić, M., Božunović, J., Nestorović Živković, J., Gašić, U., Milutinović, M., Dmitrović, S., Petrović, L., Filipović, B., Banjanac, T., Šiler, B., Anđelković, B., Dimitrijević, M.,& Mišić, D.. (2023). Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
Belgrade: Faculty of Chemistry..
https://hdl.handle.net/21.15107/rcub_ibiss_6196

Aničić N, Matekalo D, Skorić M, Božunović J, Nestorović Živković J, Gašić U, Milutinović M, Dmitrović S, Petrović L, Filipović B, Banjanac T, Šiler B, Anđelković B, Dimitrijević M, Mišić D. Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. 2023;:null-156.
https://hdl.handle.net/21.15107/rcub_ibiss_6196 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Božunović, Jelena, Nestorović Živković, Jasmina, Gašić, Uroš, Milutinović, Milica, Dmitrović, Slavica, Petrović, Luka, Filipović, Biljana, Banjanac, Tijana, Šiler, Branislav, Anđelković, Boban, Dimitrijević, Milena, Mišić, Danijela, "Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme" in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6196 .

TY  - CONF
AU  - Matekalo, Dragana
AU  - Aničić, Neda
AU  - Gašić, Uroš
AU  - Skorić, Marijana
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6082
AB  - The genus Nepeta is characterized by a fascinating diversity of iridoid compounds. Some species within the genus are characterized by the presence of both iridoid glycosides (IGs) and iridoid aglycones (IAs), while others produce exclusively IGs, or completely lack iridoids. The absence of certain iridoids does not necessarily imply the absence of gene transcripts dedicated to the particular steps of their biosynthetic pathway. Studying the biosynthetic pathways of iridoids in chemodiverse Nepeta species, while taking into account tissue-specific profiles of metabolites and relevant genes expression, may give us further insight into the mechanisms of regulation of the biosynthesis of these compounds. In this work we performed a comparative transcriptomic analysis of glandular trichomes and abraded leaves of Nepeta rtanjensis, an IAs- and IGs –rich species, and of IGs-producing N. grandiflora. The results of this analysis provided us with very informative data that can be used for parallel study of iridoid-related biosynthetic genes (BGs) and transcription factors (TFs) of the two chemodiverse taxa. On the other hand, differentially expressed genes in the investigated tissues, enabled us to generate a list of candidate genes for yet unelucidated steps of the iridoid metabolic pathway. These results combined with state-of-the-art metabolomics data bring us closer to understanding the molecular background of iridoid diversity within the genus Nepeta, and point to the factors involved in the regulation of the two distinct branches of the iridoid biosynthetic pathway, leading to either IGs or IAs.
PB  - Davis: University of California
C3  - Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
T1  - Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6082
ER  - 

@conference{
author = "Matekalo, Dragana and Aničić, Neda and Gašić, Uroš and Skorić, Marijana and Šiler, Branislav and Banjanac, Tijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Petrović, Luka and Todorović, Miloš and Lukić, Tamara and Mišić, Danijela",
year = "2023",
abstract = "The genus Nepeta is characterized by a fascinating diversity of iridoid compounds. Some species within the genus are characterized by the presence of both iridoid glycosides (IGs) and iridoid aglycones (IAs), while others produce exclusively IGs, or completely lack iridoids. The absence of certain iridoids does not necessarily imply the absence of gene transcripts dedicated to the particular steps of their biosynthetic pathway. Studying the biosynthetic pathways of iridoids in chemodiverse Nepeta species, while taking into account tissue-specific profiles of metabolites and relevant genes expression, may give us further insight into the mechanisms of regulation of the biosynthesis of these compounds. In this work we performed a comparative transcriptomic analysis of glandular trichomes and abraded leaves of Nepeta rtanjensis, an IAs- and IGs –rich species, and of IGs-producing N. grandiflora. The results of this analysis provided us with very informative data that can be used for parallel study of iridoid-related biosynthetic genes (BGs) and transcription factors (TFs) of the two chemodiverse taxa. On the other hand, differentially expressed genes in the investigated tissues, enabled us to generate a list of candidate genes for yet unelucidated steps of the iridoid metabolic pathway. These results combined with state-of-the-art metabolomics data bring us closer to understanding the molecular background of iridoid diversity within the genus Nepeta, and point to the factors involved in the regulation of the two distinct branches of the iridoid biosynthetic pathway, leading to either IGs or IAs.",
publisher = "Davis: University of California",
journal = "Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA",
title = "Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6082"
}

Matekalo, D., Aničić, N., Gašić, U., Skorić, M., Šiler, B., Banjanac, T., Nestorović Živković, J., Dmitrović, S., Filipović, B., Milutinović, M., Božunović, J., Petrović, L., Todorović, M., Lukić, T.,& Mišić, D.. (2023). Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
Davis: University of California., 82.
https://hdl.handle.net/21.15107/rcub_ibiss_6082

Matekalo D, Aničić N, Gašić U, Skorić M, Šiler B, Banjanac T, Nestorović Živković J, Dmitrović S, Filipović B, Milutinović M, Božunović J, Petrović L, Todorović M, Lukić T, Mišić D. Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. 2023;:82.
https://hdl.handle.net/21.15107/rcub_ibiss_6082 .

Matekalo, Dragana, Aničić, Neda, Gašić, Uroš, Skorić, Marijana, Šiler, Branislav, Banjanac, Tijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, Mišić, Danijela, "Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data" in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA (2023):82,
https://hdl.handle.net/21.15107/rcub_ibiss_6082 .

TY  - CONF
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Gašić, Uroš
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6081
AB  - Within a large iridoids-lacking clade of Nepetoidae (Lamiaceae), the genus Nepeta stands out as an exclusive producer of these atypical monoterpenes with a unique stereochemistry. The genus Nepeta exhibits a high diversity of iridoids, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. Comparative metabolomics, transcriptomics, gene co-expression, and phylogenetic analysis were conducted on the trans,cis-NL-rich N. rtanjensis Diklić & Milojević, the cis,trans-NL-dominant N. sibirica L., and the iridoid-lacking N. nervosa Royle & Bentham, revealing significant structural and functional differences in their iridoid synthases (ISY). N. rtanjensis was found to have two orthologues of ISY (NrISY1 and NrISY2), while N. sibirica and N. nervosa each had only one ISY (NsISY and NnISY, respectively). Although the phylogenetic analysis grouped NrISY1, NsISY, and NnISY with non-active Family 1 isoforms, organ-specific expression highlighted NrISY2, NsISY, and NnISY as promising ISY candidates. In vitro assays confirmed their function and NrISY2 was the most efficient in converting 8-oxogeranial to cis,trans-nepetalactol. However, 3D modeling and molecular docking analyses suggested that NnISY may have a unique cyclase activity. Likely, under in vivo conditions, the NnISY lacks substrates to catalyze, as a result of the loss of upstream geraniol synthase (GES) activity, which results in the loss of iridoids in N. nervosa. Presumably, some of the downstream pathway genes (NEPSs and MLPLs), essential for the NL biosynthesis, also underwent structural and activity changes in this species. Our ongoing work is conducted towards testing these robust hypotheses and re-establishing iridoid biosynthesis in N. nervosa.
PB  - Davis: University of California
C3  - Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
T1  - Functional iridoid synthases from three Nepeta species with differing iridoid  profiles
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6081
ER  - 

@conference{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Gašić, Uroš and Dimitrijević, Milena and Anđelković, Boban and Božunović, Jelena and Milutinović, Milica and Petrović, Luka and Mišić, Danijela",
year = "2023",
abstract = "Within a large iridoids-lacking clade of Nepetoidae (Lamiaceae), the genus Nepeta stands out as an exclusive producer of these atypical monoterpenes with a unique stereochemistry. The genus Nepeta exhibits a high diversity of iridoids, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. Comparative metabolomics, transcriptomics, gene co-expression, and phylogenetic analysis were conducted on the trans,cis-NL-rich N. rtanjensis Diklić & Milojević, the cis,trans-NL-dominant N. sibirica L., and the iridoid-lacking N. nervosa Royle & Bentham, revealing significant structural and functional differences in their iridoid synthases (ISY). N. rtanjensis was found to have two orthologues of ISY (NrISY1 and NrISY2), while N. sibirica and N. nervosa each had only one ISY (NsISY and NnISY, respectively). Although the phylogenetic analysis grouped NrISY1, NsISY, and NnISY with non-active Family 1 isoforms, organ-specific expression highlighted NrISY2, NsISY, and NnISY as promising ISY candidates. In vitro assays confirmed their function and NrISY2 was the most efficient in converting 8-oxogeranial to cis,trans-nepetalactol. However, 3D modeling and molecular docking analyses suggested that NnISY may have a unique cyclase activity. Likely, under in vivo conditions, the NnISY lacks substrates to catalyze, as a result of the loss of upstream geraniol synthase (GES) activity, which results in the loss of iridoids in N. nervosa. Presumably, some of the downstream pathway genes (NEPSs and MLPLs), essential for the NL biosynthesis, also underwent structural and activity changes in this species. Our ongoing work is conducted towards testing these robust hypotheses and re-establishing iridoid biosynthesis in N. nervosa.",
publisher = "Davis: University of California",
journal = "Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA",
title = "Functional iridoid synthases from three Nepeta species with differing iridoid  profiles",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6081"
}

Aničić, N., Matekalo, D., Skorić, M., Nestorović Živković, J., Dmitrović, S., Gašić, U., Dimitrijević, M., Anđelković, B., Božunović, J., Milutinović, M., Petrović, L.,& Mišić, D.. (2023). Functional iridoid synthases from three Nepeta species with differing iridoid  profiles. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
Davis: University of California., 80.
https://hdl.handle.net/21.15107/rcub_ibiss_6081

Aničić N, Matekalo D, Skorić M, Nestorović Živković J, Dmitrović S, Gašić U, Dimitrijević M, Anđelković B, Božunović J, Milutinović M, Petrović L, Mišić D. Functional iridoid synthases from three Nepeta species with differing iridoid  profiles. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. 2023;:80.
https://hdl.handle.net/21.15107/rcub_ibiss_6081 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Gašić, Uroš, Dimitrijević, Milena, Anđelković, Boban, Božunović, Jelena, Milutinović, Milica, Petrović, Luka, Mišić, Danijela, "Functional iridoid synthases from three Nepeta species with differing iridoid  profiles" in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA (2023):80,
https://hdl.handle.net/21.15107/rcub_ibiss_6081 .

TY  - CONF
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Šiler, Branislav
AU  - Dmitrović, Slavica
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5561
AB  - The genus Nepeta, one of the largest genera of the Lamiaceae family (subfamily Nepetoidae, tribe Nepeteae) comprises around 250 herbaceous, perennial, rarely annual species, native to temperate Europe, Asia, North Africa, and the mountainous region of tropical Africa. Phytochemical investigations have revealed monoterpenes (iridoids), diterpenes, triterpenes, as well as phenolics, including flavonoids, to be the major specialized metabolites in these remarkable plants. The chemical richness of terpenoids and phenolics in catmints plays functional roles in nature, facilitating complex interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavor additives, fragrances, and toys for cats, and their extensively studied bioactivities are mainly assigned to iridoid monoterpenoids nepetalactones. These compounds are proven repellents against insects (e.g. mosquitos, cockroaches, ticks, flies, mites, termites), and promising bioherbicides against a vast array of weeds (e.g. amaranth, ragweed, goosefoot, dandelion).
In spite of the plenitude of fundamental research that has documented the vast bioactive potential of Nepeta species, their commercial exploitation is restrained. There are several important factors that make the commercialization of Nepeta sp. most challenging, including limited bioresources, limited availability of bioactive compounds in purified forms, challenges to optimize the cultivation and standardize the quality of plant material, low number of studies focusing on the effects on non-target organisms and on the mutual relationships of individual compounds, instability and relatively low retainability of volatile nepetalactones, and others.
The challenge ahead is to provide sustainable sources of bioactive compounds of Nepeta sp. and thus establish a background for their commercialization. One of the solutions might be the creation of alternative sources of these biomolecules through synthetic biology approaches, thereby developing microbial or plant systems producing bioactive compounds of interest. Essential prerequisites to achieve this is the comprehensive and simultaneous acquiring, analyzing and interpreting the overall diversity of specialized metabolites within the genus Nepeta, reconstruction of the molecular background of the chemical diversity and elucidation of the biosynthetic pathways, as well as adopting the biotechnology tools to scale-up the production of the compounds of interest.
PB  - Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences
C3  - Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria
T1  - Catmints (Nepeta sp.): Biological activities and applicative potential
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5561
ER  - 

@conference{
author = "Mišić, Danijela and Gašić, Uroš and Šiler, Branislav and Dmitrović, Slavica and Skorić, Marijana and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Aničić, Neda and Petrović, Luka and Todorović, Miloš and Lukić, Tamara",
year = "2023",
abstract = "The genus Nepeta, one of the largest genera of the Lamiaceae family (subfamily Nepetoidae, tribe Nepeteae) comprises around 250 herbaceous, perennial, rarely annual species, native to temperate Europe, Asia, North Africa, and the mountainous region of tropical Africa. Phytochemical investigations have revealed monoterpenes (iridoids), diterpenes, triterpenes, as well as phenolics, including flavonoids, to be the major specialized metabolites in these remarkable plants. The chemical richness of terpenoids and phenolics in catmints plays functional roles in nature, facilitating complex interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavor additives, fragrances, and toys for cats, and their extensively studied bioactivities are mainly assigned to iridoid monoterpenoids nepetalactones. These compounds are proven repellents against insects (e.g. mosquitos, cockroaches, ticks, flies, mites, termites), and promising bioherbicides against a vast array of weeds (e.g. amaranth, ragweed, goosefoot, dandelion).
In spite of the plenitude of fundamental research that has documented the vast bioactive potential of Nepeta species, their commercial exploitation is restrained. There are several important factors that make the commercialization of Nepeta sp. most challenging, including limited bioresources, limited availability of bioactive compounds in purified forms, challenges to optimize the cultivation and standardize the quality of plant material, low number of studies focusing on the effects on non-target organisms and on the mutual relationships of individual compounds, instability and relatively low retainability of volatile nepetalactones, and others.
The challenge ahead is to provide sustainable sources of bioactive compounds of Nepeta sp. and thus establish a background for their commercialization. One of the solutions might be the creation of alternative sources of these biomolecules through synthetic biology approaches, thereby developing microbial or plant systems producing bioactive compounds of interest. Essential prerequisites to achieve this is the comprehensive and simultaneous acquiring, analyzing and interpreting the overall diversity of specialized metabolites within the genus Nepeta, reconstruction of the molecular background of the chemical diversity and elucidation of the biosynthetic pathways, as well as adopting the biotechnology tools to scale-up the production of the compounds of interest.",
publisher = "Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences",
journal = "Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria",
title = "Catmints (Nepeta sp.): Biological activities and applicative potential",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5561"
}

Mišić, D., Gašić, U., Šiler, B., Dmitrović, S., Skorić, M., Matekalo, D., Nestorović Živković, J., Banjanac, T., Filipović, B., Milutinović, M., Božunović, J., Aničić, N., Petrović, L., Todorović, M.,& Lukić, T.. (2023). Catmints (Nepeta sp.): Biological activities and applicative potential. in Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria
Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_5561

Mišić D, Gašić U, Šiler B, Dmitrović S, Skorić M, Matekalo D, Nestorović Živković J, Banjanac T, Filipović B, Milutinović M, Božunović J, Aničić N, Petrović L, Todorović M, Lukić T. Catmints (Nepeta sp.): Biological activities and applicative potential. in Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria. 2023;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_5561 .

Mišić, Danijela, Gašić, Uroš, Šiler, Branislav, Dmitrović, Slavica, Skorić, Marijana, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Aničić, Neda, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, "Catmints (Nepeta sp.): Biological activities and applicative potential" in Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria (2023):18,
https://hdl.handle.net/21.15107/rcub_ibiss_5561 .

TY  - JOUR
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6456
AB  - Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.
PB  - Lausanne, Switzerland: Frontiers
T2  - Frontiers in Plant Science
T1  - Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)
VL  - 14
DO  - 10.3389/fpls.2023.1211453
SP  - 1211453
ER  - 

@article{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Božunović, Jelena and Milutinović, Milica and Petrović, Luka and Dimitrijević, Milena and Anđelković, Boban and Mišić, Danijela",
year = "2023",
abstract = "Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.",
publisher = "Lausanne, Switzerland: Frontiers",
journal = "Frontiers in Plant Science",
title = "Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)",
volume = "14",
doi = "10.3389/fpls.2023.1211453",
pages = "1211453"
}

Aničić, N., Matekalo, D., Skorić, M., Gašić, U., Nestorović Živković, J., Dmitrović, S., Božunović, J., Milutinović, M., Petrović, L., Dimitrijević, M., Anđelković, B.,& Mišić, D.. (2023). Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae). in Frontiers in Plant Science
Lausanne, Switzerland: Frontiers., 14, 1211453.
https://doi.org/10.3389/fpls.2023.1211453

Aničić N, Matekalo D, Skorić M, Gašić U, Nestorović Živković J, Dmitrović S, Božunović J, Milutinović M, Petrović L, Dimitrijević M, Anđelković B, Mišić D. Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae). in Frontiers in Plant Science. 2023;14:1211453.
doi:10.3389/fpls.2023.1211453 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Božunović, Jelena, Milutinović, Milica, Petrović, Luka, Dimitrijević, Milena, Anđelković, Boban, Mišić, Danijela, "Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)" in Frontiers in Plant Science, 14 (2023):1211453,
https://doi.org/10.3389/fpls.2023.1211453 . .

TY  - JOUR
AU  - Mattoo, Autar
AU  - Cavigelli, Michel A.
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Maksimović, Vuk
AU  - Kramer, Matthew
AU  - Kaur, Bhavneet
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Roberts, Daniel
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6255
AB  - Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.
PB  - Frontiers
T2  - Frontiers in Sustainable Food Systems
T1  - Maize metabolomics in relation to cropping system and growing year
VL  - 7
DO  - 10.3389/fsufs.2023.1130089
SP  - 1130089
ER  - 

@article{
author = "Mattoo, Autar and Cavigelli, Michel A. and Mišić, Danijela and Gašić, Uroš and Maksimović, Vuk and Kramer, Matthew and Kaur, Bhavneet and Matekalo, Dragana and Nestorović Živković, Jasmina and Roberts, Daniel",
year = "2023",
abstract = "Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.",
publisher = "Frontiers",
journal = "Frontiers in Sustainable Food Systems",
title = "Maize metabolomics in relation to cropping system and growing year",
volume = "7",
doi = "10.3389/fsufs.2023.1130089",
pages = "1130089"
}

Mattoo, A., Cavigelli, M. A., Mišić, D., Gašić, U., Maksimović, V., Kramer, M., Kaur, B., Matekalo, D., Nestorović Živković, J.,& Roberts, D.. (2023). Maize metabolomics in relation to cropping system and growing year. in Frontiers in Sustainable Food Systems
Frontiers., 7, 1130089.
https://doi.org/10.3389/fsufs.2023.1130089

Mattoo A, Cavigelli MA, Mišić D, Gašić U, Maksimović V, Kramer M, Kaur B, Matekalo D, Nestorović Živković J, Roberts D. Maize metabolomics in relation to cropping system and growing year. in Frontiers in Sustainable Food Systems. 2023;7:1130089.
doi:10.3389/fsufs.2023.1130089 .

Mattoo, Autar, Cavigelli, Michel A., Mišić, Danijela, Gašić, Uroš, Maksimović, Vuk, Kramer, Matthew, Kaur, Bhavneet, Matekalo, Dragana, Nestorović Živković, Jasmina, Roberts, Daniel, "Maize metabolomics in relation to cropping system and growing year" in Frontiers in Sustainable Food Systems, 7 (2023):1130089,
https://doi.org/10.3389/fsufs.2023.1130089 . .

TY  - JOUR
AU  - Alimpić-Aradski, Ana
AU  - Olađe Pavlović, Mariana
AU  - Janošević, Dušica
AU  - Todorović, Slađana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Pljevljakušić, Dejan
AU  - Šavikin, Katarina
AU  - Marin, Petar D.
AU  - Giweli, Abdulhmid
AU  - Duletić-Laušević, Sonja
PY  - 2023
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/5152
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6445
AB  - Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.
PB  - Hoboken: John Wiley and Sons
T2  - Phytochemical Analysis
T1  - Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)
IS  - 6
VL  - 34
DO  - 10.1002/pca.3257
SP  - 661
EP  - 679
ER  - 

@article{
author = "Alimpić-Aradski, Ana and Olađe Pavlović, Mariana and Janošević, Dušica and Todorović, Slađana and Gašić, Uroš and Mišić, Danijela and Pljevljakušić, Dejan and Šavikin, Katarina and Marin, Petar D. and Giweli, Abdulhmid and Duletić-Laušević, Sonja",
year = "2023",
abstract = "Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Phytochemical Analysis",
title = "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)",
number = "6",
volume = "34",
doi = "10.1002/pca.3257",
pages = "661-679"
}

Alimpić-Aradski, A., Olađe Pavlović, M., Janošević, D., Todorović, S., Gašić, U., Mišić, D., Pljevljakušić, D., Šavikin, K., Marin, P. D., Giweli, A.,& Duletić-Laušević, S.. (2023). Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis
Hoboken: John Wiley and Sons., 34(6), 661-679.
https://doi.org/10.1002/pca.3257

Alimpić-Aradski A, Olađe Pavlović M, Janošević D, Todorović S, Gašić U, Mišić D, Pljevljakušić D, Šavikin K, Marin PD, Giweli A, Duletić-Laušević S. Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis. 2023;34(6):661-679.
doi:10.1002/pca.3257 .

Alimpić-Aradski, Ana, Olađe Pavlović, Mariana, Janošević, Dušica, Todorović, Slađana, Gašić, Uroš, Mišić, Danijela, Pljevljakušić, Dejan, Šavikin, Katarina, Marin, Petar D., Giweli, Abdulhmid, Duletić-Laušević, Sonja, "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)" in Phytochemical Analysis, 34, no. 6 (2023):661-679,
https://doi.org/10.1002/pca.3257 . .

TY  - JOUR
AU  - Hadi, Najmeh
AU  - Nikolić, Miloš
AU  - Sefidkon, Fatemeh
AU  - Shojaeiyan, Abdolali
AU  - Šiler, Branislav
AU  - Mišić, Danijela
AU  - Yahyazadeh, Mahdi
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6440
AB  - Pathogenic microorganisms are more or less successfully treated by synthetic chemical compounds, whose residues often cause serious health problems. Plant specialized metabolites with antimicrobial properties have for a long time been the focus of both medicine and pharmacology. This study was conducted to evaluate the in vitro antimicrobial activity of methanol extracts of selected endemic and native Iranian Nepeta species against some of the most important pathogenic bacteria and fungi. The results indicated that N. kotschyi leaf extract was the most efficient against the tested bacteria, with Pseudomonas aeruginosa being the most sensitive and fungal species were more susceptible to the extracts than bacterial strains. Nepeta spp. extracts showed a strong antifungal activity against micromycetes, except for quite resistant Aspergillus niger. Antibacterial MIC values (mg.mL-1) ranged from 0.01 (N. kotschyi) to 0.20 (N. crassifolia), while antifungal MIC values ranged from 0.02 (N. crassifolia, N. kotschyi, N. menthoides, and N. cataria) to 0.13 (N. crassifolia and N. menthoides). When compared to positive controls, in most cases the extracts performed much better. The recorded antimicrobial activity candidates the selected 4 endemic and native Iranian Nepeta spp. as prospective and promising antimicrobial agents to be used in both pharmacology and biotechnology.
PB  - CMB Association Publishers
T2  - Cellular and Molecular Biology
T1  - Native and endemic Iranian Nepeta spp.: powerful antimicrobial agents
IS  - 69
VL  - 10
DO  - 10.14715/cmb/2023.69.10.18
SP  - 129
EP  - 135
ER  - 

@article{
author = "Hadi, Najmeh and Nikolić, Miloš and Sefidkon, Fatemeh and Shojaeiyan, Abdolali and Šiler, Branislav and Mišić, Danijela and Yahyazadeh, Mahdi",
year = "2023",
abstract = "Pathogenic microorganisms are more or less successfully treated by synthetic chemical compounds, whose residues often cause serious health problems. Plant specialized metabolites with antimicrobial properties have for a long time been the focus of both medicine and pharmacology. This study was conducted to evaluate the in vitro antimicrobial activity of methanol extracts of selected endemic and native Iranian Nepeta species against some of the most important pathogenic bacteria and fungi. The results indicated that N. kotschyi leaf extract was the most efficient against the tested bacteria, with Pseudomonas aeruginosa being the most sensitive and fungal species were more susceptible to the extracts than bacterial strains. Nepeta spp. extracts showed a strong antifungal activity against micromycetes, except for quite resistant Aspergillus niger. Antibacterial MIC values (mg.mL-1) ranged from 0.01 (N. kotschyi) to 0.20 (N. crassifolia), while antifungal MIC values ranged from 0.02 (N. crassifolia, N. kotschyi, N. menthoides, and N. cataria) to 0.13 (N. crassifolia and N. menthoides). When compared to positive controls, in most cases the extracts performed much better. The recorded antimicrobial activity candidates the selected 4 endemic and native Iranian Nepeta spp. as prospective and promising antimicrobial agents to be used in both pharmacology and biotechnology.",
publisher = "CMB Association Publishers",
journal = "Cellular and Molecular Biology",
title = "Native and endemic Iranian Nepeta spp.: powerful antimicrobial agents",
number = "69",
volume = "10",
doi = "10.14715/cmb/2023.69.10.18",
pages = "129-135"
}

Hadi, N., Nikolić, M., Sefidkon, F., Shojaeiyan, A., Šiler, B., Mišić, D.,& Yahyazadeh, M.. (2023). Native and endemic Iranian Nepeta spp.: powerful antimicrobial agents. in Cellular and Molecular Biology
CMB Association Publishers., 10(69), 129-135.
https://doi.org/10.14715/cmb/2023.69.10.18

Hadi N, Nikolić M, Sefidkon F, Shojaeiyan A, Šiler B, Mišić D, Yahyazadeh M. Native and endemic Iranian Nepeta spp.: powerful antimicrobial agents. in Cellular and Molecular Biology. 2023;10(69):129-135.
doi:10.14715/cmb/2023.69.10.18 .

Hadi, Najmeh, Nikolić, Miloš, Sefidkon, Fatemeh, Shojaeiyan, Abdolali, Šiler, Branislav, Mišić, Danijela, Yahyazadeh, Mahdi, "Native and endemic Iranian Nepeta spp.: powerful antimicrobial agents" in Cellular and Molecular Biology, 10, no. 69 (2023):129-135,
https://doi.org/10.14715/cmb/2023.69.10.18 . .

TY  - CONF
AU  - Lukić, Tamara
AU  - Šiler, Branislav
AU  - Mišić, Danijela
AU  - Nestorović Živković, Jasmina
AU  - Jelić, Mihailo
AU  - Brkušanin, Miloš
AU  - Filipović, Biljana
AU  - Todorović, Miloš
AU  - Banjanac, Tijana
PY  - 2023
UR  - https://europlantbiology2023.org/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6444
AB  - The species of the genus Centaurium Hill are characterized by a pronounced phenotypic
plasticity, while interspecific hybridization readily occurs. Previous studies reported hybridization events between C. erythraea and C. tenuiflorum in the Mediterranean region,
but the status of a potentially hybrid taxon (termed "Centaurium ×tenuiflorum") remains
unclear. Therefore, molecular markers (EST-SSR, ITS, and chloroplast DNA markers) were
combined and their usefulness in estimating overall genetic variability and in identifying
phylogenetic relations between Centaurium taxa was scrutinized. Both EST-SSR and ITS
markers proved to be well suited to distinguish the parental taxa C. erythraea and C.
tenuiflorum, and a putative hybridogenic taxon "C. ×tenuiflorum", the last one being more
related to C. erythraea. Conversely, maternally inherited chloroplast markers (trnL-F, rbcL
and psbA-trnH) nominated C. tenuiflorum as a donor of plastid.
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Molecular markers in the detection of interspecies  hybridization – a model study on the genus Centaurium Hill
SP  - 79
EP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6444
ER  - 

@conference{
author = "Lukić, Tamara and Šiler, Branislav and Mišić, Danijela and Nestorović Živković, Jasmina and Jelić, Mihailo and Brkušanin, Miloš and Filipović, Biljana and Todorović, Miloš and Banjanac, Tijana",
year = "2023",
abstract = "The species of the genus Centaurium Hill are characterized by a pronounced phenotypic
plasticity, while interspecific hybridization readily occurs. Previous studies reported hybridization events between C. erythraea and C. tenuiflorum in the Mediterranean region,
but the status of a potentially hybrid taxon (termed "Centaurium ×tenuiflorum") remains
unclear. Therefore, molecular markers (EST-SSR, ITS, and chloroplast DNA markers) were
combined and their usefulness in estimating overall genetic variability and in identifying
phylogenetic relations between Centaurium taxa was scrutinized. Both EST-SSR and ITS
markers proved to be well suited to distinguish the parental taxa C. erythraea and C.
tenuiflorum, and a putative hybridogenic taxon "C. ×tenuiflorum", the last one being more
related to C. erythraea. Conversely, maternally inherited chloroplast markers (trnL-F, rbcL
and psbA-trnH) nominated C. tenuiflorum as a donor of plastid.",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Molecular markers in the detection of interspecies  hybridization – a model study on the genus Centaurium Hill",
pages = "79-80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6444"
}

Lukić, T., Šiler, B., Mišić, D., Nestorović Živković, J., Jelić, M., Brkušanin, M., Filipović, B., Todorović, M.,& Banjanac, T.. (2023). Molecular markers in the detection of interspecies  hybridization – a model study on the genus Centaurium Hill. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 79-80.
https://hdl.handle.net/21.15107/rcub_ibiss_6444

Lukić T, Šiler B, Mišić D, Nestorović Živković J, Jelić M, Brkušanin M, Filipović B, Todorović M, Banjanac T. Molecular markers in the detection of interspecies  hybridization – a model study on the genus Centaurium Hill. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:79-80.
https://hdl.handle.net/21.15107/rcub_ibiss_6444 .

Lukić, Tamara, Šiler, Branislav, Mišić, Danijela, Nestorović Živković, Jasmina, Jelić, Mihailo, Brkušanin, Miloš, Filipović, Biljana, Todorović, Miloš, Banjanac, Tijana, "Molecular markers in the detection of interspecies  hybridization – a model study on the genus Centaurium Hill" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):79-80,
https://hdl.handle.net/21.15107/rcub_ibiss_6444 .

TY  - JOUR
AU  - Rajčić, Marija
AU  - Ćosić, Marija
AU  - Tosti, Tomislav
AU  - Mišić, Danijela
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Vujičić, Milorad
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6258
AB  - The same population of the polytrichaceous moss Polytrichum formosum was 
studied over four different periods of the year, analysing its carbohydrate and 
polyphenolic content and dynamics related to environmental seasonal changes. A 
total of 18 different types of sugars (including mono-, di-, tri- and tetra-saccha rides) and four sugar alcohols were determined. Chlorogenic acid was the most 
represented among the 10 detected phenolic compounds. As inferred by the sugar 
content, sucrose, fructose and glucose were the most dominant sugars, but it is 
worth mentioning the abundance of trehalose and turanose at least during one of 
the observed seasons. The presence of four trisaccharides and one tetrasaccharide 
within P. formosum should be highlighted, as well as the first reports of turanose, 
isomaltotriose, panose and rhamnose within this species. The quantitative changes 
over the year clearly demonstrate carbohydrate dynamics in relation to seasonal 
climatic variation. Sugars are shown to be significant constitutive molecules within 
P. formosum, but also physiologically active compounds, i.e. signalling and energy 
storage and supplier molecules. We assume that phenolics have moss-supportive 
effects during oxidative stress and biotic interaction.
PB  - Belgrade: Institute of Botany and Botanical Garden "Jevremovac"
T2  - Botanica Serbica
T1  - An insight into seasonal changes of carbohydrates and phenolic compounds within the moss Polytrichum formosum (Polytrichaceae)
IS  - 1
VL  - 47
DO  - 10.2298/BOTSERB2301125R
SP  - 125
EP  - 133
ER  - 

@article{
author = "Rajčić, Marija and Ćosić, Marija and Tosti, Tomislav and Mišić, Danijela and Sabovljević, Aneta and Sabovljević, Marko and Vujičić, Milorad",
year = "2023",
abstract = "The same population of the polytrichaceous moss Polytrichum formosum was 
studied over four different periods of the year, analysing its carbohydrate and 
polyphenolic content and dynamics related to environmental seasonal changes. A 
total of 18 different types of sugars (including mono-, di-, tri- and tetra-saccha rides) and four sugar alcohols were determined. Chlorogenic acid was the most 
represented among the 10 detected phenolic compounds. As inferred by the sugar 
content, sucrose, fructose and glucose were the most dominant sugars, but it is 
worth mentioning the abundance of trehalose and turanose at least during one of 
the observed seasons. The presence of four trisaccharides and one tetrasaccharide 
within P. formosum should be highlighted, as well as the first reports of turanose, 
isomaltotriose, panose and rhamnose within this species. The quantitative changes 
over the year clearly demonstrate carbohydrate dynamics in relation to seasonal 
climatic variation. Sugars are shown to be significant constitutive molecules within 
P. formosum, but also physiologically active compounds, i.e. signalling and energy 
storage and supplier molecules. We assume that phenolics have moss-supportive 
effects during oxidative stress and biotic interaction.",
publisher = "Belgrade: Institute of Botany and Botanical Garden "Jevremovac"",
journal = "Botanica Serbica",
title = "An insight into seasonal changes of carbohydrates and phenolic compounds within the moss Polytrichum formosum (Polytrichaceae)",
number = "1",
volume = "47",
doi = "10.2298/BOTSERB2301125R",
pages = "125-133"
}

Rajčić, M., Ćosić, M., Tosti, T., Mišić, D., Sabovljević, A., Sabovljević, M.,& Vujičić, M.. (2023). An insight into seasonal changes of carbohydrates and phenolic compounds within the moss Polytrichum formosum (Polytrichaceae). in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden "Jevremovac"., 47(1), 125-133.
https://doi.org/10.2298/BOTSERB2301125R

Rajčić M, Ćosić M, Tosti T, Mišić D, Sabovljević A, Sabovljević M, Vujičić M. An insight into seasonal changes of carbohydrates and phenolic compounds within the moss Polytrichum formosum (Polytrichaceae). in Botanica Serbica. 2023;47(1):125-133.
doi:10.2298/BOTSERB2301125R .

Rajčić, Marija, Ćosić, Marija, Tosti, Tomislav, Mišić, Danijela, Sabovljević, Aneta, Sabovljević, Marko, Vujičić, Milorad, "An insight into seasonal changes of carbohydrates and phenolic compounds within the moss Polytrichum formosum (Polytrichaceae)" in Botanica Serbica, 47, no. 1 (2023):125-133,
https://doi.org/10.2298/BOTSERB2301125R . .

TY  - CONF
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Mišić, Danijela
AU  - Nakarada, Đura
AU  - Mojović, Miloš
AU  - Jovanović, Olivera
AU  - Škoro, Nikola
AU  - Puač, Nevena
PY  - 2023
UR  - https://europlantbiology2023.org/wp-content/uploads/2023/07/PBE2023-Abstract-Book.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6224
AB  - Non-thermal plasma (NTP) technology offers a promising future in plant metabolic engineering, being energy efficient and Eco-friendly alternative to the conventional treatments [1]. Plasma environment is enriched with reactive oxygen and nitrogen species (RONS) that participate in various signaling pathways in plants by regulating their metabolic and developmental processes. In the present study calli of different carrot (Daucus carota L.) varieties was treated by using plasma needle device designed for biomedical applications [2]. Metabolite profiling revealed that plasma treatment could induce severe qualitative and quantitative changes of the major phenolic compounds detected in carrot calli. Current
metabolic alteration was followed by the significant shift in the antioxidant capacity of the treated calli. Obtained results outline the potential application of plasma treatment as a novel elicitor for the production of bio-active compounds in plant in vitro culture systems
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment
SP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6224
ER  - 

@conference{
author = "Živković, Suzana and Milutinović, Milica and Mišić, Danijela and Nakarada, Đura and Mojović, Miloš and Jovanović, Olivera and Škoro, Nikola and Puač, Nevena",
year = "2023",
abstract = "Non-thermal plasma (NTP) technology offers a promising future in plant metabolic engineering, being energy efficient and Eco-friendly alternative to the conventional treatments [1]. Plasma environment is enriched with reactive oxygen and nitrogen species (RONS) that participate in various signaling pathways in plants by regulating their metabolic and developmental processes. In the present study calli of different carrot (Daucus carota L.) varieties was treated by using plasma needle device designed for biomedical applications [2]. Metabolite profiling revealed that plasma treatment could induce severe qualitative and quantitative changes of the major phenolic compounds detected in carrot calli. Current
metabolic alteration was followed by the significant shift in the antioxidant capacity of the treated calli. Obtained results outline the potential application of plasma treatment as a novel elicitor for the production of bio-active compounds in plant in vitro culture systems",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment",
pages = "154",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6224"
}

Živković, S., Milutinović, M., Mišić, D., Nakarada, Đ., Mojović, M., Jovanović, O., Škoro, N.,& Puač, N.. (2023). Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 154.
https://hdl.handle.net/21.15107/rcub_ibiss_6224

Živković S, Milutinović M, Mišić D, Nakarada Đ, Mojović M, Jovanović O, Škoro N, Puač N. Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:154.
https://hdl.handle.net/21.15107/rcub_ibiss_6224 .

Živković, Suzana, Milutinović, Milica, Mišić, Danijela, Nakarada, Đura, Mojović, Miloš, Jovanović, Olivera, Škoro, Nikola, Puač, Nevena, "Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):154,
https://hdl.handle.net/21.15107/rcub_ibiss_6224 .

TY  - JOUR
AU  - Gašić, Uroš
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Dmitrović, Slavica
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Živković, Suzana
AU  - Matekalo, Dragana
AU  - Filipović, Biljana
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2023.1155297/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5494
AB  - The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species ( D. lanata , D. ferruginea , and D. grandiflora ) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis ( Plantaginaceae ) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora . The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p -coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p -coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea . However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis .
T2  - Frontiers in Plant Science
T1  - Variation in the chemical profiles of three foxglove species in the central Balkans
VL  - 14
DO  - 10.3389/fpls.2023.1155297
ER  - 

@article{
author = "Gašić, Uroš and Banjanac, Tijana and Šiler, Branislav and Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Dmitrović, Slavica and Skorić, Marijana and Nestorović Živković, Jasmina and Petrović, Luka and Todorović, Miloš and Živković, Suzana and Matekalo, Dragana and Filipović, Biljana and Lukić, Tamara and Mišić, Danijela",
year = "2023",
abstract = "The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species ( D. lanata , D. ferruginea , and D. grandiflora ) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis ( Plantaginaceae ) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora . The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p -coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p -coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea . However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis .",
journal = "Frontiers in Plant Science",
title = "Variation in the chemical profiles of three foxglove species in the central Balkans",
volume = "14",
doi = "10.3389/fpls.2023.1155297"
}

Gašić, U., Banjanac, T., Šiler, B., Božunović, J., Milutinović, M., Aničić, N., Dmitrović, S., Skorić, M., Nestorović Živković, J., Petrović, L., Todorović, M., Živković, S., Matekalo, D., Filipović, B., Lukić, T.,& Mišić, D.. (2023). Variation in the chemical profiles of three foxglove species in the central Balkans. in Frontiers in Plant Science, 14.
https://doi.org/10.3389/fpls.2023.1155297

Gašić U, Banjanac T, Šiler B, Božunović J, Milutinović M, Aničić N, Dmitrović S, Skorić M, Nestorović Živković J, Petrović L, Todorović M, Živković S, Matekalo D, Filipović B, Lukić T, Mišić D. Variation in the chemical profiles of three foxglove species in the central Balkans. in Frontiers in Plant Science. 2023;14.
doi:10.3389/fpls.2023.1155297 .

Gašić, Uroš, Banjanac, Tijana, Šiler, Branislav, Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Dmitrović, Slavica, Skorić, Marijana, Nestorović Živković, Jasmina, Petrović, Luka, Todorović, Miloš, Živković, Suzana, Matekalo, Dragana, Filipović, Biljana, Lukić, Tamara, Mišić, Danijela, "Variation in the chemical profiles of three foxglove species in the central Balkans" in Frontiers in Plant Science, 14 (2023),
https://doi.org/10.3389/fpls.2023.1155297 . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Todorović, Miloš
AU  - Gašić, Uroš
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ivković, Đurđa
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Aničić, Neda
AU  - Banjanac, Tijana
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5505
AB  - The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening
IS  - 2
VL  - 12
DO  - 10.3390/antiox12020346
SP  - 346
ER  - 

@article{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Todorović, Miloš and Gašić, Uroš and Živković, Suzana and Skorić, Marijana and Ivković, Đurđa and Savić, Jelena and Devrnja, Nina and Aničić, Neda and Banjanac, Tijana and Mojović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening",
number = "2",
volume = "12",
doi = "10.3390/antiox12020346",
pages = "346"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Todorović, M., Gašić, U., Živković, S., Skorić, M., Ivković, Đ., Savić, J., Devrnja, N., Aničić, N., Banjanac, T., Mojović, M.,& Mišić, D.. (2023). Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants
Basel: MDPI., 12(2), 346.
https://doi.org/10.3390/antiox12020346

Milutinović M, Nakarada Đ, Božunović J, Todorović M, Gašić U, Živković S, Skorić M, Ivković Đ, Savić J, Devrnja N, Aničić N, Banjanac T, Mojović M, Mišić D. Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants. 2023;12(2):346.
doi:10.3390/antiox12020346 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Todorović, Miloš, Gašić, Uroš, Živković, Suzana, Skorić, Marijana, Ivković, Đurđa, Savić, Jelena, Devrnja, Nina, Aničić, Neda, Banjanac, Tijana, Mojović, Miloš, Mišić, Danijela, "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening" in Antioxidants, 12, no. 2 (2023):346,
https://doi.org/10.3390/antiox12020346 . .

TY  - JOUR
AU  - Ćosić, Marija
AU  - Mišić, Danijela
AU  - Jakovljević, Ksenija
AU  - Giba, Zlatko
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Vujičić, Milorad
PY  - 2023
UR  - https://www.mdpi.com/1420-3049/28/4/1794
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36838781
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9967137
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5493
AB  - The response to salt stress analysed by quantitative and qualitative analyses in three selected moss species was studied. Non-halophytic funaroid Physcomitrium patens and two halophytic mosses, funaroid Entosthodon hungaricus and pottioid Hennediella heimii were exposed to salt stress under controlled in vitro conditions. The results clearly showed various phenolics to be present and included to some extent as a non-enzymatic component of oxidative, i.e., salt stress. The common pattern of responses characteristic of phenolic compounds was not present in these moss species, but in all three species the role of phenolics to stress tolerance was documented. The phenolic p-coumaric acid detected in all three species is assumed to be a common phenolic included in the antioxidative response and salt-stress tolerance. Although the stress response in each species also included other phenolics, the mechanisms were different, and also dependent on the stress intensity and duration.
PB  - Basel: MDPI
T2  - Molecules (Basel, Switzerland)
T1  - Analysis of the Qualitative and Quantitative Content of the Phenolic Compounds of Selected Moss Species under NaCl Stress
IS  - 4
VL  - 28
DO  - 10.3390/molecules28041794
SP  - 1794
ER  - 

@article{
author = "Ćosić, Marija and Mišić, Danijela and Jakovljević, Ksenija and Giba, Zlatko and Sabovljević, Aneta and Sabovljević, Marko and Vujičić, Milorad",
year = "2023",
abstract = "The response to salt stress analysed by quantitative and qualitative analyses in three selected moss species was studied. Non-halophytic funaroid Physcomitrium patens and two halophytic mosses, funaroid Entosthodon hungaricus and pottioid Hennediella heimii were exposed to salt stress under controlled in vitro conditions. The results clearly showed various phenolics to be present and included to some extent as a non-enzymatic component of oxidative, i.e., salt stress. The common pattern of responses characteristic of phenolic compounds was not present in these moss species, but in all three species the role of phenolics to stress tolerance was documented. The phenolic p-coumaric acid detected in all three species is assumed to be a common phenolic included in the antioxidative response and salt-stress tolerance. Although the stress response in each species also included other phenolics, the mechanisms were different, and also dependent on the stress intensity and duration.",
publisher = "Basel: MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "Analysis of the Qualitative and Quantitative Content of the Phenolic Compounds of Selected Moss Species under NaCl Stress",
number = "4",
volume = "28",
doi = "10.3390/molecules28041794",
pages = "1794"
}

Ćosić, M., Mišić, D., Jakovljević, K., Giba, Z., Sabovljević, A., Sabovljević, M.,& Vujičić, M.. (2023). Analysis of the Qualitative and Quantitative Content of the Phenolic Compounds of Selected Moss Species under NaCl Stress. in Molecules (Basel, Switzerland)
Basel: MDPI., 28(4), 1794.
https://doi.org/10.3390/molecules28041794

Ćosić M, Mišić D, Jakovljević K, Giba Z, Sabovljević A, Sabovljević M, Vujičić M. Analysis of the Qualitative and Quantitative Content of the Phenolic Compounds of Selected Moss Species under NaCl Stress. in Molecules (Basel, Switzerland). 2023;28(4):1794.
doi:10.3390/molecules28041794 .

Ćosić, Marija, Mišić, Danijela, Jakovljević, Ksenija, Giba, Zlatko, Sabovljević, Aneta, Sabovljević, Marko, Vujičić, Milorad, "Analysis of the Qualitative and Quantitative Content of the Phenolic Compounds of Selected Moss Species under NaCl Stress" in Molecules (Basel, Switzerland), 28, no. 4 (2023):1794,
https://doi.org/10.3390/molecules28041794 . .

TY  - JOUR
AU  - Božunović, Jelena
AU  - Ivanov, Marija
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Nakarada, Đura
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Giba, Zlatko
AU  - Mišić, Danijela
AU  - Stojković, Dejan
PY  - 2023
UR  - https://www.mdpi.com/1424-8247/16/2/245
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5490
AB  - Spiked centaury (Centaurium spicatum) is a well-known medicinal plant from the Mediterranean region with various bioactivities, but there are no studies addressing the use of different solvent systems to improve its pharmacological potential. Nine extraction procedures were adapted to study the effects of solvent composition on the content of bioactive compounds in C. spicatum extracts and on corresponding bioactivities. Targeted metabolomics was performed to obtain information on the chemical composition of extracts. Ethanol-water-based extraction procedures were the most efficient in isolating polyphenols, while less polar butanol extract contained the highest amount of iridoids. Antioxidant potential analysis revealed stronger activity in extracts with higher polyphenol content. Bacillus cereus and Staphylococus aureus were designated as the most sensitive bacterial strains to the activity of extracts, while among the micromycetes tested, Penicillium funiculosum was the most susceptible strain. Butanol extract showed antivirulence potential on Candida albicans morphological transition from yeast to hyphal form, and selected extracts were effective against biofilm formation in two Candida species. All the extracts tested in this study showed no cytotoxic activity to immortalize human skin keratinocyte cell line (HaCaT), whereas extracts obtained by ethanol-water extraction stand out for their potent wound healing effects. Moreover, the influence of the extraction solvent system on various bioactivities of C. spicatum is reported herein for the first time. Overall, the results presented in this study promote the use of C. spicatum as a source of natural products with potential antioxidant, wound healing, and antimicrobial applications that are potentially safe for human use.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts
IS  - 2
VL  - 16
DO  - 10.3390/ph16020245
SP  - 245
ER  - 

@article{
author = "Božunović, Jelena and Ivanov, Marija and Petrović, Jovana and Gašić, Uroš and Nakarada, Đura and Milutinović, Milica and Aničić, Neda and Giba, Zlatko and Mišić, Danijela and Stojković, Dejan",
year = "2023",
abstract = "Spiked centaury (Centaurium spicatum) is a well-known medicinal plant from the Mediterranean region with various bioactivities, but there are no studies addressing the use of different solvent systems to improve its pharmacological potential. Nine extraction procedures were adapted to study the effects of solvent composition on the content of bioactive compounds in C. spicatum extracts and on corresponding bioactivities. Targeted metabolomics was performed to obtain information on the chemical composition of extracts. Ethanol-water-based extraction procedures were the most efficient in isolating polyphenols, while less polar butanol extract contained the highest amount of iridoids. Antioxidant potential analysis revealed stronger activity in extracts with higher polyphenol content. Bacillus cereus and Staphylococus aureus were designated as the most sensitive bacterial strains to the activity of extracts, while among the micromycetes tested, Penicillium funiculosum was the most susceptible strain. Butanol extract showed antivirulence potential on Candida albicans morphological transition from yeast to hyphal form, and selected extracts were effective against biofilm formation in two Candida species. All the extracts tested in this study showed no cytotoxic activity to immortalize human skin keratinocyte cell line (HaCaT), whereas extracts obtained by ethanol-water extraction stand out for their potent wound healing effects. Moreover, the influence of the extraction solvent system on various bioactivities of C. spicatum is reported herein for the first time. Overall, the results presented in this study promote the use of C. spicatum as a source of natural products with potential antioxidant, wound healing, and antimicrobial applications that are potentially safe for human use.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts",
number = "2",
volume = "16",
doi = "10.3390/ph16020245",
pages = "245"
}

Božunović, J., Ivanov, M., Petrović, J., Gašić, U., Nakarada, Đ., Milutinović, M., Aničić, N., Giba, Z., Mišić, D.,& Stojković, D.. (2023). Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts. in Pharmaceuticals
Basel: MDPI., 16(2), 245.
https://doi.org/10.3390/ph16020245

Božunović J, Ivanov M, Petrović J, Gašić U, Nakarada Đ, Milutinović M, Aničić N, Giba Z, Mišić D, Stojković D. Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts. in Pharmaceuticals. 2023;16(2):245.
doi:10.3390/ph16020245 .

Božunović, Jelena, Ivanov, Marija, Petrović, Jovana, Gašić, Uroš, Nakarada, Đura, Milutinović, Milica, Aničić, Neda, Giba, Zlatko, Mišić, Danijela, Stojković, Dejan, "Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts" in Pharmaceuticals, 16, no. 2 (2023):245,
https://doi.org/10.3390/ph16020245 . .

TY  - DATA
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5448
AB  - Leaves from the flowering stems of three Digitalis species (Digitalis grandiflora, D. lanata, and D. ferruginea), originating from 28 populations from the Central Balkan Peninsula, were subjected to targeted metabolomics. Totally 16 compounds from the groups of phenolics (phenolic acids and flavonoids) and cardenolides were quantified in analyzed samples, and values are presented as mg/L of extract.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Metabolomics quantitative data of three foxglove species (genus Digitalis, fam. Plantaginaceae) from Central Balkan Peninsula
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5448
ER  - 

@misc{
author = "Gašić, Uroš and Mišić, Danijela and Šiler, Branislav and Banjanac, Tijana",
year = "2023",
abstract = "Leaves from the flowering stems of three Digitalis species (Digitalis grandiflora, D. lanata, and D. ferruginea), originating from 28 populations from the Central Balkan Peninsula, were subjected to targeted metabolomics. Totally 16 compounds from the groups of phenolics (phenolic acids and flavonoids) and cardenolides were quantified in analyzed samples, and values are presented as mg/L of extract.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Metabolomics quantitative data of three foxglove species (genus Digitalis, fam. Plantaginaceae) from Central Balkan Peninsula",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5448"
}

Gašić, U., Mišić, D., Šiler, B.,& Banjanac, T.. (2023). Metabolomics quantitative data of three foxglove species (genus Digitalis, fam. Plantaginaceae) from Central Balkan Peninsula. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5448

Gašić U, Mišić D, Šiler B, Banjanac T. Metabolomics quantitative data of three foxglove species (genus Digitalis, fam. Plantaginaceae) from Central Balkan Peninsula. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5448 .

Gašić, Uroš, Mišić, Danijela, Šiler, Branislav, Banjanac, Tijana, "Metabolomics quantitative data of three foxglove species (genus Digitalis, fam. Plantaginaceae) from Central Balkan Peninsula" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5448 .

TY  - JOUR
AU  - Katanić Stanković, Jelena S.
AU  - Đorović Jovanović, Jelena
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Nikles, Stefanie
AU  - Marković, Zoran
AU  - Bauer, Rudolf
PY  - 2023
UR  - https://www.mdpi.com/1420-3049/28/3/1274
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9921532
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5482
AB  - Rabelera holostea (L.) M. T. Sharples & E. A. Tripp (Greater Stitchwort), formerly known as Stellaria holostea L., is widespread in the warm temperate areas of Europe and Western Asia, the Caucasus region, as well as in some countries of North Africa. Nowadays it is considered as a weed, but earlier it was often used raw in salads or for the treatment of various inflammatory disorders. The goal of this study was to determine the constituents of the methanol extract of R. holostea aerial parts and its biological potential in terms of antioxidant, antimicrobial, and anti-inflammatory properties. Until now, the constituents and biological activities of this plant were not reported in detail. A comprehensive phytochemical profiling of the extract has shown that phenolic acids, such as ferulic, chlorogenic, and p-coumaric acid, flavonoids and flavonoid glucosides, such as chrysoeriol, rutin, and naringin, are the most abundant compounds. The antioxidant activity of R. holostea extract towards DPPH and ABTS radicals, but also the total antioxidant capacity and the inhibition of lipid peroxidation were moderate. The antimicrobial potential was pronounced mostly towards some fungi such as F. oxysporum (MIC 1.25 mg/mL), whereas the capacity of R. holostea to affect the growth of bacteria was much less pronounced. R. holostea extract was most inclined to anti-inflammatory activity. At a concentration of 50 µg/mL, it significantly inhibited both cyclooxygenase enzymes (COX-1 and COX-2) by 71.24% and 72.83%, respectively. Molecular docking studies indicated that chlorogenic acid and chrysoeriol are the main contributors to COX-1 and COX-2 inhibitory activity.
PB  - Basel: MDPI
T2  - Molecules (Basel, Switzerland)
T1  - UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract
IS  - 3
VL  - 28
DO  - 10.3390/molecules28031274
SP  - 1274
ER  - 

@article{
author = "Katanić Stanković, Jelena S. and Đorović Jovanović, Jelena and Mišić, Danijela and Gašić, Uroš and Nikles, Stefanie and Marković, Zoran and Bauer, Rudolf",
year = "2023",
abstract = "Rabelera holostea (L.) M. T. Sharples & E. A. Tripp (Greater Stitchwort), formerly known as Stellaria holostea L., is widespread in the warm temperate areas of Europe and Western Asia, the Caucasus region, as well as in some countries of North Africa. Nowadays it is considered as a weed, but earlier it was often used raw in salads or for the treatment of various inflammatory disorders. The goal of this study was to determine the constituents of the methanol extract of R. holostea aerial parts and its biological potential in terms of antioxidant, antimicrobial, and anti-inflammatory properties. Until now, the constituents and biological activities of this plant were not reported in detail. A comprehensive phytochemical profiling of the extract has shown that phenolic acids, such as ferulic, chlorogenic, and p-coumaric acid, flavonoids and flavonoid glucosides, such as chrysoeriol, rutin, and naringin, are the most abundant compounds. The antioxidant activity of R. holostea extract towards DPPH and ABTS radicals, but also the total antioxidant capacity and the inhibition of lipid peroxidation were moderate. The antimicrobial potential was pronounced mostly towards some fungi such as F. oxysporum (MIC 1.25 mg/mL), whereas the capacity of R. holostea to affect the growth of bacteria was much less pronounced. R. holostea extract was most inclined to anti-inflammatory activity. At a concentration of 50 µg/mL, it significantly inhibited both cyclooxygenase enzymes (COX-1 and COX-2) by 71.24% and 72.83%, respectively. Molecular docking studies indicated that chlorogenic acid and chrysoeriol are the main contributors to COX-1 and COX-2 inhibitory activity.",
publisher = "Basel: MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract",
number = "3",
volume = "28",
doi = "10.3390/molecules28031274",
pages = "1274"
}

Katanić Stanković, J. S., Đorović Jovanović, J., Mišić, D., Gašić, U., Nikles, S., Marković, Z.,& Bauer, R.. (2023). UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract. in Molecules (Basel, Switzerland)
Basel: MDPI., 28(3), 1274.
https://doi.org/10.3390/molecules28031274

Katanić Stanković JS, Đorović Jovanović J, Mišić D, Gašić U, Nikles S, Marković Z, Bauer R. UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract. in Molecules (Basel, Switzerland). 2023;28(3):1274.
doi:10.3390/molecules28031274 .

Katanić Stanković, Jelena S., Đorović Jovanović, Jelena, Mišić, Danijela, Gašić, Uroš, Nikles, Stefanie, Marković, Zoran, Bauer, Rudolf, "UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract" in Molecules (Basel, Switzerland), 28, no. 3 (2023):1274,
https://doi.org/10.3390/molecules28031274 . .

TY  - CONF
AU  - Petrović, Luka
AU  - Skorić, Marijana
AU  - Aničić, Neda
AU  - Todorović, Miloš
AU  - Matekalo, Dragana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Lukić, Tamara
AU  - Filipović, Biljana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5568
AB  - Nepeta nuda L. (familija Lamiaceae) je, pored N. rtanjensis i N. cataria, jedna od tri vrste roda Nepeta koje rastu u Srbiji, naseljavajući prvenstveno sušna staništa na obodima šuma planinskih masiva. Poput većine predstavnika ovog roda, vrstu N. nuda karakteriše prisustvo različitih specijalizovanih metabolita, prvenstveno iridoida i fenolnih jedinjenja. Listovi jedinki N. nuda, prikupljeni su na različitim lokalitetima u istočnoj Srbiji: Izvor (Paraćin), Rtanj, Ozren i Barta Berilovac (Stara planina). Kvalitativni i kvantitativni hemijski sastav prikupljenog biljnog materijala analiziran je UHPLC/DAD/(±)HESI-MS2 metodom, usmerenom na dominantna bioaktivna jedinjenja iz grupa iridoida i polifenola. Rezultati metaboličkog profilisanja iridoida pokazali su da jedinke iz analiziranih populacija dominantno produkuju iridoidni glikozid - epideoksiloganinsku kiselinu, dok aglikoni iridoida nisu detektovani. Predstavnici svih ispitivanih populacija od fenolnih jedinjenja dominantno sintetišu ruzmarinsku kiselinu, dok su u tragovima detektovana jedinjenja kao što su kafeinska kiselina, izokvercitrin i astragalin. Sadržaj analiziranih jedinjenja varira između analiziranih populacija N. nuda. Populacija sa lokaliteta Balta Berilovac izdvaja se po visokom sadržaju epideoksiloganinske kiseline, što je čini interesantnom za dalja ispitivanja bioloških aktivnosti.
AB  - Nepeta nuda L. (фамилија Lamiaceae) је, поред N. rtanjensis и N. cataria, једна од три врсте рода Nepeta које расту у Србији, насељавајући првенствено сушна станишта на ободима шума планинских масива. Попут већине представника овог рода, врсту N. nuda карактерише присуство различитих специјализованих метаболита, првенствено иридоида и фенолних једињења. Листови јединки N. nuda, прикупљени су на различитим локалитетима у источној Србији: Извор (Параћин), Ртањ, Озрен и Барта Бериловац (Стара планина). Квалитативни и квантитативни хемијски састав прикупљеног биљног материјала анализиран је UHPLC/DAD/(±)HESI-MS2 методом, усмереном на доминантна биоактивна једињења из група иридоида и полифенола. Резултати метаболичког профилисања иридоида показали су да јединке из анализираних популација доминантно продукују иридоидни гликозид - епидеоксилоганинску киселину, док агликони иридоида нису детектовани. Представници свих испитиваних популација од фенолних једињења доминантно синтетишу рузмаринску киселину, док су у траговима детектована једињења као што су кафеинска киселина, изокверцитрин и астрагалин. Садржај анализираних једињења варира између анализираних популација N. nuda. Популација са локалитета Балта Бериловац издваја се по високом садржају епидеоксилоганинске киселине, што је чини интересантном за даља испитивања биолошких активности.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Хемијска карактеризација популација врсте Nepeta nuda L. из источне Србије
T1  - Hemijska karakterizacija populacija vrste Nepeta nuda L. iz istočne Srbije
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5568
ER  - 

@conference{
author = "Petrović, Luka and Skorić, Marijana and Aničić, Neda and Todorović, Miloš and Matekalo, Dragana and Milutinović, Milica and Božunović, Jelena and Nestorović Živković, Jasmina and Dmitrović, Slavica and Šiler, Branislav and Banjanac, Tijana and Lukić, Tamara and Filipović, Biljana and Gašić, Uroš and Mišić, Danijela",
year = "2022",
abstract = "Nepeta nuda L. (familija Lamiaceae) je, pored N. rtanjensis i N. cataria, jedna od tri vrste roda Nepeta koje rastu u Srbiji, naseljavajući prvenstveno sušna staništa na obodima šuma planinskih masiva. Poput većine predstavnika ovog roda, vrstu N. nuda karakteriše prisustvo različitih specijalizovanih metabolita, prvenstveno iridoida i fenolnih jedinjenja. Listovi jedinki N. nuda, prikupljeni su na različitim lokalitetima u istočnoj Srbiji: Izvor (Paraćin), Rtanj, Ozren i Barta Berilovac (Stara planina). Kvalitativni i kvantitativni hemijski sastav prikupljenog biljnog materijala analiziran je UHPLC/DAD/(±)HESI-MS2 metodom, usmerenom na dominantna bioaktivna jedinjenja iz grupa iridoida i polifenola. Rezultati metaboličkog profilisanja iridoida pokazali su da jedinke iz analiziranih populacija dominantno produkuju iridoidni glikozid - epideoksiloganinsku kiselinu, dok aglikoni iridoida nisu detektovani. Predstavnici svih ispitivanih populacija od fenolnih jedinjenja dominantno sintetišu ruzmarinsku kiselinu, dok su u tragovima detektovana jedinjenja kao što su kafeinska kiselina, izokvercitrin i astragalin. Sadržaj analiziranih jedinjenja varira između analiziranih populacija N. nuda. Populacija sa lokaliteta Balta Berilovac izdvaja se po visokom sadržaju epideoksiloganinske kiseline, što je čini interesantnom za dalja ispitivanja bioloških aktivnosti., Nepeta nuda L. (фамилија Lamiaceae) је, поред N. rtanjensis и N. cataria, једна од три врсте рода Nepeta које расту у Србији, насељавајући првенствено сушна станишта на ободима шума планинских масива. Попут већине представника овог рода, врсту N. nuda карактерише присуство различитих специјализованих метаболита, првенствено иридоида и фенолних једињења. Листови јединки N. nuda, прикупљени су на различитим локалитетима у источној Србији: Извор (Параћин), Ртањ, Озрен и Барта Бериловац (Стара планина). Квалитативни и квантитативни хемијски састав прикупљеног биљног материјала анализиран је UHPLC/DAD/(±)HESI-MS2 методом, усмереном на доминантна биоактивна једињења из група иридоида и полифенола. Резултати метаболичког профилисања иридоида показали су да јединке из анализираних популација доминантно продукују иридоидни гликозид - епидеоксилоганинску киселину, док агликони иридоида нису детектовани. Представници свих испитиваних популација од фенолних једињења доминантно синтетишу рузмаринску киселину, док су у траговима детектована једињења као што су кафеинска киселина, изокверцитрин и астрагалин. Садржај анализираних једињења варира између анализираних популација N. nuda. Популација са локалитета Балта Бериловац издваја се по високом садржају епидеоксилоганинске киселине, што је чини интересантном за даља испитивања биолошких активности.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Хемијска карактеризација популација врсте Nepeta nuda L. из источне Србије, Hemijska karakterizacija populacija vrste Nepeta nuda L. iz istočne Srbije",
pages = "61",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5568"
}

Petrović, L., Skorić, M., Aničić, N., Todorović, M., Matekalo, D., Milutinović, M., Božunović, J., Nestorović Živković, J., Dmitrović, S., Šiler, B., Banjanac, T., Lukić, T., Filipović, B., Gašić, U.,& Mišić, D.. (2022). Хемијска карактеризација популација врсте Nepeta nuda L. из источне Србије. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 61.
https://hdl.handle.net/21.15107/rcub_ibiss_5568

Petrović L, Skorić M, Aničić N, Todorović M, Matekalo D, Milutinović M, Božunović J, Nestorović Živković J, Dmitrović S, Šiler B, Banjanac T, Lukić T, Filipović B, Gašić U, Mišić D. Хемијска карактеризација популација врсте Nepeta nuda L. из источне Србије. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:61.
https://hdl.handle.net/21.15107/rcub_ibiss_5568 .

Petrović, Luka, Skorić, Marijana, Aničić, Neda, Todorović, Miloš, Matekalo, Dragana, Milutinović, Milica, Božunović, Jelena, Nestorović Živković, Jasmina, Dmitrović, Slavica, Šiler, Branislav, Banjanac, Tijana, Lukić, Tamara, Filipović, Biljana, Gašić, Uroš, Mišić, Danijela, "Хемијска карактеризација популација врсте Nepeta nuda L. из источне Србије" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):61,
https://hdl.handle.net/21.15107/rcub_ibiss_5568 .

TY  - CONF
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Aničić, Neda
AU  - Milutinović, Milica
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Šiler, Branislav
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Matekalo, Dragana
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Dragićević, Milan
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5564
AB  - The aim of this study was to determine qualitative composition of methanol-soluble metabolites in leaves
from in vitro-grown plants of phylogenetically distinct Nepeta species producing iridoids (N. sibirica L., N.
spicata Benth., N. argolica Bory & Chaub., and N. rtanjensis Diklić & Milojević) and iridoid-lacking N. nervosa
Royle ex Benth., adopting UHPLC-LTQ OrbiTrap MS technique. Totally, 52 compounds were identified
based on their monoisotopic masses, MSn fragmentation, and previously reported MS data and they were
clustered into four major groups: phenolic acid derivatives (23 compounds), flavonoids (10 compounds),
iridoids (13 compounds), and 6 compounds belonging to other classes. Both phenolic and iridoid profiles
of the tested species were found to be very diverse regarding both qualitative content of compounds and
their relative representation in extracts. Rosmarinic, 5-O-cafeoylquinic, and caffeic acids were found to be
the dominant phenolics in all analyzed species, especially in N. argolica, N. rtanjensis, and N. nervosa.
Iridoids were present in the form of glucosides and the most abundant compounds from this group were
epideoxyloganic acid, geniposide, and aucubin. Although iridoids were not detected in N. nervosa extracts,
this species was revealed as a rich source of phenolic acids and flavonoids. Conversely, N. spicata was found
to produce iridoids but negligible amounts of phenolic compounds. Extracts of two closely related endemic
species originating from the Balkan Peninsula, N. argolica and N. rtanjensis, were very similar in both
phenolic and iridoid profiles. The UHPLC-LTQ OrbiTrap MS method, in combination with chemometrics
data processing, was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic
determination and chemical differentiation of Nepeta species.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
T1  - Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5564
ER  - 

@conference{
author = "Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Aničić, Neda and Milutinović, Milica and Skorić, Marijana and Božunović, Jelena and Petrović, Luka and Šiler, Branislav and Filipović, Biljana and Banjanac, Tijana and Matekalo, Dragana and Todorović, Miloš and Lukić, Tamara and Dragićević, Milan and Mišić, Danijela",
year = "2022",
abstract = "The aim of this study was to determine qualitative composition of methanol-soluble metabolites in leaves
from in vitro-grown plants of phylogenetically distinct Nepeta species producing iridoids (N. sibirica L., N.
spicata Benth., N. argolica Bory & Chaub., and N. rtanjensis Diklić & Milojević) and iridoid-lacking N. nervosa
Royle ex Benth., adopting UHPLC-LTQ OrbiTrap MS technique. Totally, 52 compounds were identified
based on their monoisotopic masses, MSn fragmentation, and previously reported MS data and they were
clustered into four major groups: phenolic acid derivatives (23 compounds), flavonoids (10 compounds),
iridoids (13 compounds), and 6 compounds belonging to other classes. Both phenolic and iridoid profiles
of the tested species were found to be very diverse regarding both qualitative content of compounds and
their relative representation in extracts. Rosmarinic, 5-O-cafeoylquinic, and caffeic acids were found to be
the dominant phenolics in all analyzed species, especially in N. argolica, N. rtanjensis, and N. nervosa.
Iridoids were present in the form of glucosides and the most abundant compounds from this group were
epideoxyloganic acid, geniposide, and aucubin. Although iridoids were not detected in N. nervosa extracts,
this species was revealed as a rich source of phenolic acids and flavonoids. Conversely, N. spicata was found
to produce iridoids but negligible amounts of phenolic compounds. Extracts of two closely related endemic
species originating from the Balkan Peninsula, N. argolica and N. rtanjensis, were very similar in both
phenolic and iridoid profiles. The UHPLC-LTQ OrbiTrap MS method, in combination with chemometrics
data processing, was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic
determination and chemical differentiation of Nepeta species.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia",
title = "Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5564"
}

Gašić, U., Nestorović Živković, J., Dmitrović, S., Aničić, N., Milutinović, M., Skorić, M., Božunović, J., Petrović, L., Šiler, B., Filipović, B., Banjanac, T., Matekalo, D., Todorović, M., Lukić, T., Dragićević, M.,& Mišić, D.. (2022). Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
Ljubljana: Slovenian Society of Plant Biology., 35.
https://hdl.handle.net/21.15107/rcub_ibiss_5564

Gašić U, Nestorović Živković J, Dmitrović S, Aničić N, Milutinović M, Skorić M, Božunović J, Petrović L, Šiler B, Filipović B, Banjanac T, Matekalo D, Todorović M, Lukić T, Dragićević M, Mišić D. Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. 2022;:35.
https://hdl.handle.net/21.15107/rcub_ibiss_5564 .

Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Aničić, Neda, Milutinović, Milica, Skorić, Marijana, Božunović, Jelena, Petrović, Luka, Šiler, Branislav, Filipović, Biljana, Banjanac, Tijana, Matekalo, Dragana, Todorović, Miloš, Lukić, Tamara, Dragićević, Milan, Mišić, Danijela, "Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry" in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia (2022):35,
https://hdl.handle.net/21.15107/rcub_ibiss_5564 .